LOCUS       DADLTI010000001       100721 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLTI010000001 DADLTI010000000
VERSION     DADLTI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 100721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.001"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..258
                     /locus_tag="JN755_000001"
     CDS             <1..258
                     /locus_tag="JN755_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY7378931.1"
                     /translation="VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENL
                     FAIGSVLGGFDPIAQGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            255..1445
                     /gene="glpC"
                     /locus_tag="JN755_000002"
     CDS             255..1445
                     /gene="glpC"
                     /locus_tag="JN755_000002"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7378932.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            1638..2537
                     /locus_tag="JN755_000003"
     CDS             1638..2537
                     /locus_tag="JN755_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7378933.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            2556..2777
                     /locus_tag="JN755_000004"
     CDS             2556..2777
                     /locus_tag="JN755_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7378934.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(2818..3288)
                     /locus_tag="JN755_000005"
     CDS             complement(2818..3288)
                     /locus_tag="JN755_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7378935.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(3343..4545)
                     /locus_tag="JN755_000006"
     CDS             complement(3343..4545)
                     /locus_tag="JN755_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7378936.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(4632..5174)
                     /locus_tag="JN755_000007"
     CDS             complement(4632..5174)
                     /locus_tag="JN755_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7378937.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            5453..5878
                     /gene="nudI"
                     /locus_tag="JN755_000008"
     CDS             5453..5878
                     /gene="nudI"
                     /locus_tag="JN755_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7378938.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(5917..6519)
                     /locus_tag="JN755_000009"
     CDS             complement(5917..6519)
                     /locus_tag="JN755_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7378939.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            6827..7966
                     /gene="arnB"
                     /locus_tag="JN755_000010"
     CDS             6827..7966
                     /gene="arnB"
                     /locus_tag="JN755_000010"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7378940.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            7970..8938
                     /gene="arnC"
                     /locus_tag="JN755_000011"
     CDS             7970..8938
                     /gene="arnC"
                     /locus_tag="JN755_000011"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7378941.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLSSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            8938..10920
                     /gene="arnA"
                     /locus_tag="JN755_000012"
     CDS             8938..10920
                     /gene="arnA"
                     /locus_tag="JN755_000012"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7378942.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            10917..11807
                     /locus_tag="JN755_000013"
     CDS             10917..11807
                     /locus_tag="JN755_000013"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7378943.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            11807..13459
                     /gene="arnT"
                     /locus_tag="JN755_000014"
     CDS             11807..13459
                     /gene="arnT"
                     /locus_tag="JN755_000014"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7378944.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            13456..13791
                     /gene="arnE"
                     /locus_tag="JN755_000015"
     CDS             13456..13791
                     /gene="arnE"
                     /locus_tag="JN755_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7378945.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            13791..14177
                     /gene="arnF"
                     /locus_tag="JN755_000016"
     CDS             13791..14177
                     /gene="arnF"
                     /locus_tag="JN755_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7378946.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(14171..14443)
                     /gene="pmrD"
                     /locus_tag="JN755_000017"
     CDS             complement(14171..14443)
                     /gene="pmrD"
                     /locus_tag="JN755_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7378947.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(14548..15903)
                     /gene="menE"
                     /locus_tag="JN755_000018"
     CDS             complement(14548..15903)
                     /gene="menE"
                     /locus_tag="JN755_000018"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7378948.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(15900..16862)
                     /gene="menC"
                     /locus_tag="JN755_000019"
     CDS             complement(15900..16862)
                     /gene="menC"
                     /locus_tag="JN755_000019"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7378949.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(16862..17719)
                     /gene="menB"
                     /locus_tag="JN755_000020"
     CDS             complement(16862..17719)
                     /gene="menB"
                     /locus_tag="JN755_000020"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7378950.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(17734..18492)
                     /gene="menH"
                     /locus_tag="JN755_000021"
     CDS             complement(17734..18492)
                     /gene="menH"
                     /locus_tag="JN755_000021"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7378951.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(18489..20159)
                     /gene="menD"
                     /locus_tag="JN755_000022"
     CDS             complement(18489..20159)
                     /gene="menD"
                     /locus_tag="JN755_000022"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7378952.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HCPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(20248..21543)
                     /gene="menF"
                     /locus_tag="JN755_000023"
     CDS             complement(20248..21543)
                     /gene="menF"
                     /locus_tag="JN755_000023"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7378953.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(21622..21927)
                     /gene="elaB"
                     /locus_tag="JN755_000024"
     CDS             complement(21622..21927)
                     /gene="elaB"
                     /locus_tag="JN755_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7378954.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(21982..22443)
                     /locus_tag="JN755_000025"
     CDS             complement(21982..22443)
                     /locus_tag="JN755_000025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7378955.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            22508..23425
                     /gene="rbn"
                     /locus_tag="JN755_000026"
     CDS             22508..23425
                     /gene="rbn"
                     /locus_tag="JN755_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7378956.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            23613..24833
                     /gene="elaD"
                     /locus_tag="JN755_000027"
     CDS             23613..24833
                     /gene="elaD"
                     /locus_tag="JN755_000027"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY7378957.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            25376..25868
                     /locus_tag="JN755_000028"
                     /pseudo
     CDS             25376..25868
                     /locus_tag="JN755_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            25971..26474
                     /locus_tag="JN755_000029"
     CDS             25971..26474
                     /locus_tag="JN755_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7378958.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(26541..27998)
                     /gene="nuoN"
                     /locus_tag="JN755_000030"
     CDS             complement(26541..27998)
                     /gene="nuoN"
                     /locus_tag="JN755_000030"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7378959.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(28005..29534)
                     /gene="nuoM"
                     /locus_tag="JN755_000031"
     CDS             complement(28005..29534)
                     /gene="nuoM"
                     /locus_tag="JN755_000031"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7378960.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(29698..31539)
                     /gene="nuoL"
                     /locus_tag="JN755_000032"
     CDS             complement(29698..31539)
                     /gene="nuoL"
                     /locus_tag="JN755_000032"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7378961.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(31536..31838)
                     /gene="nuoK"
                     /locus_tag="JN755_000033"
     CDS             complement(31536..31838)
                     /gene="nuoK"
                     /locus_tag="JN755_000033"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7378962.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(31835..32389)
                     /gene="nuoJ"
                     /locus_tag="JN755_000034"
     CDS             complement(31835..32389)
                     /gene="nuoJ"
                     /locus_tag="JN755_000034"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7378963.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(32401..32943)
                     /gene="nuoI"
                     /locus_tag="JN755_000035"
     CDS             complement(32401..32943)
                     /gene="nuoI"
                     /locus_tag="JN755_000035"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7378964.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(32958..33935)
                     /gene="nuoH"
                     /locus_tag="JN755_000036"
     CDS             complement(32958..33935)
                     /gene="nuoH"
                     /locus_tag="JN755_000036"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7378965.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(33932..36658)
                     /gene="nuoG"
                     /locus_tag="JN755_000037"
     CDS             complement(33932..36658)
                     /gene="nuoG"
                     /locus_tag="JN755_000037"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7378966.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(36711..38048)
                     /gene="nuoF"
                     /locus_tag="JN755_000038"
     CDS             complement(36711..38048)
                     /gene="nuoF"
                     /locus_tag="JN755_000038"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7378967.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(38045..38545)
                     /gene="nuoE"
                     /locus_tag="JN755_000039"
     CDS             complement(38045..38545)
                     /gene="nuoE"
                     /locus_tag="JN755_000039"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7378968.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(38548..40350)
                     /gene="nuoC"
                     /locus_tag="JN755_000040"
     CDS             complement(38548..40350)
                     /gene="nuoC"
                     /locus_tag="JN755_000040"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7378969.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(40444..41106)
                     /locus_tag="JN755_000041"
     CDS             complement(40444..41106)
                     /locus_tag="JN755_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7378970.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(41122..41565)
                     /gene="nuoA"
                     /locus_tag="JN755_000042"
     CDS             complement(41122..41565)
                     /gene="nuoA"
                     /locus_tag="JN755_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7378971.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(42196..43134)
                     /gene="lrhA"
                     /locus_tag="JN755_000043"
     CDS             complement(42196..43134)
                     /gene="lrhA"
                     /locus_tag="JN755_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7378972.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            44078..45295
                     /locus_tag="JN755_000044"
     CDS             44078..45295
                     /locus_tag="JN755_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7378973.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            45379..45978
                     /gene="yfbR"
                     /locus_tag="JN755_000045"
     CDS             45379..45978
                     /gene="yfbR"
                     /locus_tag="JN755_000045"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7378974.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(46037..47869)
                     /locus_tag="JN755_000046"
     CDS             complement(46037..47869)
                     /locus_tag="JN755_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7378975.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(47956..48606)
                     /gene="hxpA"
                     /locus_tag="JN755_000047"
     CDS             complement(47956..48606)
                     /gene="hxpA"
                     /locus_tag="JN755_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7378976.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(48617..49111)
                     /locus_tag="JN755_000048"
     CDS             complement(48617..49111)
                     /locus_tag="JN755_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7378977.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(49194..49649)
                     /locus_tag="JN755_000049"
     CDS             complement(49194..49649)
                     /locus_tag="JN755_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7378978.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            49987..51189
                     /gene="ackA"
                     /locus_tag="JN755_000050"
     CDS             49987..51189
                     /gene="ackA"
                     /locus_tag="JN755_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7378979.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            51264..53408
                     /gene="pta"
                     /locus_tag="JN755_000051"
     CDS             51264..53408
                     /gene="pta"
                     /locus_tag="JN755_000051"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7378980.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            53598..55118
                     /gene="yfcC"
                     /locus_tag="JN755_000052"
     CDS             53598..55118
                     /gene="yfcC"
                     /locus_tag="JN755_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7378981.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(55151..55693)
                     /gene="yfcD"
                     /locus_tag="JN755_000053"
     CDS             complement(55151..55693)
                     /gene="yfcD"
                     /locus_tag="JN755_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7378982.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(55751..56302)
                     /gene="yfcE"
                     /locus_tag="JN755_000054"
     CDS             complement(55751..56302)
                     /gene="yfcE"
                     /locus_tag="JN755_000054"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY7378983.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(56358..57002)
                     /gene="yfcF"
                     /locus_tag="JN755_000055"
     CDS             complement(56358..57002)
                     /gene="yfcF"
                     /locus_tag="JN755_000055"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7378984.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            57138..57785
                     /gene="yfcG"
                     /locus_tag="JN755_000056"
     CDS             57138..57785
                     /gene="yfcG"
                     /locus_tag="JN755_000056"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7378985.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            57842..58204
                     /gene="folX"
                     /locus_tag="JN755_000057"
     CDS             57842..58204
                     /gene="folX"
                     /locus_tag="JN755_000057"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7378986.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            58225..59118
                     /locus_tag="JN755_000058"
     CDS             58225..59118
                     /locus_tag="JN755_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7378987.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(59166..60056)
                     /locus_tag="JN755_000059"
     CDS             complement(59166..60056)
                     /locus_tag="JN755_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7378988.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(60253..61026)
                     /gene="hisP"
                     /locus_tag="JN755_000060"
     CDS             complement(60253..61026)
                     /gene="hisP"
                     /locus_tag="JN755_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7378989.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(61034..61750)
                     /locus_tag="JN755_000061"
     CDS             complement(61034..61750)
                     /locus_tag="JN755_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7378990.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(61747..62433)
                     /locus_tag="JN755_000062"
     CDS             complement(61747..62433)
                     /locus_tag="JN755_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7378991.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(62523..63305)
                     /gene="hisJ"
                     /locus_tag="JN755_000063"
     CDS             complement(62523..63305)
                     /gene="hisJ"
                     /locus_tag="JN755_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7378992.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(63526..64308)
                     /gene="argT"
                     /locus_tag="JN755_000064"
     CDS             complement(63526..64308)
                     /gene="argT"
                     /locus_tag="JN755_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY7378993.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(64574..65143)
                     /locus_tag="JN755_000065"
     CDS             complement(64574..65143)
                     /locus_tag="JN755_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7378994.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(65238..66755)
                     /gene="purF"
                     /locus_tag="JN755_000066"
     CDS             complement(65238..66755)
                     /gene="purF"
                     /locus_tag="JN755_000066"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7378995.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(66792..67280)
                     /gene="cvpA"
                     /locus_tag="JN755_000067"
     CDS             complement(66792..67280)
                     /gene="cvpA"
                     /locus_tag="JN755_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7378996.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(67539..68201)
                     /gene="dedD"
                     /locus_tag="JN755_000068"
     CDS             complement(67539..68201)
                     /gene="dedD"
                     /locus_tag="JN755_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7378997.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(68191..69459)
                     /gene="folC"
                     /locus_tag="JN755_000069"
     CDS             complement(68191..69459)
                     /gene="folC"
                     /locus_tag="JN755_000069"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7378998.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(69529..70443)
                     /gene="accD"
                     /locus_tag="JN755_000070"
     CDS             complement(69529..70443)
                     /gene="accD"
                     /locus_tag="JN755_000070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7378999.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(70599..71258)
                     /locus_tag="JN755_000071"
     CDS             complement(70599..71258)
                     /locus_tag="JN755_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7379000.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(71341..72153)
                     /gene="truA"
                     /locus_tag="JN755_000072"
     CDS             complement(71341..72153)
                     /gene="truA"
                     /locus_tag="JN755_000072"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7379001.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(72153..73166)
                     /locus_tag="JN755_000073"
     CDS             complement(72153..73166)
                     /locus_tag="JN755_000073"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7379002.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(73232..74368)
                     /gene="pdxB"
                     /locus_tag="JN755_000074"
     CDS             complement(73232..74368)
                     /gene="pdxB"
                     /locus_tag="JN755_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7379003.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            74467..75462
                     /locus_tag="JN755_000075"
     CDS             74467..75462
                     /locus_tag="JN755_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7379004.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(75459..76637)
                     /locus_tag="JN755_000076"
     CDS             complement(75459..76637)
                     /locus_tag="JN755_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379005.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(76912..78132)
                     /gene="fabB"
                     /locus_tag="JN755_000077"
     CDS             complement(76912..78132)
                     /gene="fabB"
                     /locus_tag="JN755_000077"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7379006.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            78291..80297
                     /gene="mnmC"
                     /locus_tag="JN755_000078"
     CDS             78291..80297
                     /gene="mnmC"
                     /locus_tag="JN755_000078"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7379007.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(80418..80696)
                     /locus_tag="JN755_000079"
     CDS             complement(80418..80696)
                     /locus_tag="JN755_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7379008.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(80730..81278)
                     /locus_tag="JN755_000080"
     CDS             complement(80730..81278)
                     /locus_tag="JN755_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7379009.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(81278..82087)
                     /locus_tag="JN755_000081"
     CDS             complement(81278..82087)
                     /locus_tag="JN755_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7379010.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(82087..82911)
                     /gene="mepA"
                     /locus_tag="JN755_000082"
     CDS             complement(82087..82911)
                     /gene="mepA"
                     /locus_tag="JN755_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7379011.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(82915..84000)
                     /gene="aroC"
                     /locus_tag="JN755_000083"
     CDS             complement(82915..84000)
                     /gene="aroC"
                     /locus_tag="JN755_000083"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7379012.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(84035..84967)
                     /gene="prmB"
                     /locus_tag="JN755_000084"
     CDS             complement(84035..84967)
                     /gene="prmB"
                     /locus_tag="JN755_000084"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7379013.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            85133..85684
                     /gene="smrB"
                     /locus_tag="JN755_000085"
     CDS             85133..85684
                     /gene="smrB"
                     /locus_tag="JN755_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7379014.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(85788..86678)
                     /locus_tag="JN755_000086"
     CDS             complement(85788..86678)
                     /locus_tag="JN755_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7379015.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(86743..87189)
                     /locus_tag="JN755_000087"
     CDS             complement(86743..87189)
                     /locus_tag="JN755_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379016.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(87186..87656)
                     /locus_tag="JN755_000088"
     CDS             complement(87186..87656)
                     /locus_tag="JN755_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379017.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(87653..88201)
                     /locus_tag="JN755_000089"
     CDS             complement(87653..88201)
                     /locus_tag="JN755_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379018.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(88206..88928)
                     /locus_tag="JN755_000090"
     CDS             complement(88206..88928)
                     /locus_tag="JN755_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7379019.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(88948..91589)
                     /gene="yfcU"
                     /locus_tag="JN755_000091"
                     /pseudo
     CDS             complement(88948..91589)
                     /gene="yfcU"
                     /locus_tag="JN755_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(91671..92234)
                     /locus_tag="JN755_000092"
     CDS             complement(91671..92234)
                     /locus_tag="JN755_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7379020.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(92918..93403)
                     /gene="sixA"
                     /locus_tag="JN755_000093"
     CDS             complement(92918..93403)
                     /gene="sixA"
                     /locus_tag="JN755_000093"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7379021.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(93606..95750)
                     /gene="fadJ"
                     /locus_tag="JN755_000094"
     CDS             complement(93606..95750)
                     /gene="fadJ"
                     /locus_tag="JN755_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7379022.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(95750..97060)
                     /gene="fadI"
                     /locus_tag="JN755_000095"
     CDS             complement(95750..97060)
                     /gene="fadI"
                     /locus_tag="JN755_000095"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7379023.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(97240..97524)
                     /locus_tag="JN755_000096"
     CDS             complement(97240..97524)
                     /locus_tag="JN755_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7379024.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            97896..99236
                     /gene="fadL"
                     /locus_tag="JN755_000097"
     CDS             97896..99236
                     /gene="fadL"
                     /locus_tag="JN755_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7379025.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            99602..100660
                     /locus_tag="JN755_000098"
     CDS             99602..100660
                     /locus_tag="JN755_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379026.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        25251 a        26922 c        24785 g        23763 t
ORIGIN      
        1 gtgctacaaa ccgccacgcg gggtgaatgg tataagggcg atttttttgc gccgcaaccg
       61 tggcagcagt tcggtgtaac cactgatgag acgctacgcc cgtcgcaggc agggcaaacc
      121 attgagaacc tgtttgccat cggttcggtg ctgggcggat ttgatcccat cgcccaggga
      181 tgcggcggcg gtgtttgtgc cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc
      241 gcaggaggcc aacaatgaac gacaccagct tcgaaaactg cattaagtgc accgtctgca
      301 ccaccgcctg cccggtgagc cgggtgaatc ccggttatcc agggccaaaa caagccgggc
      361 cggatggcga gcgtctgcgt ttgaaagatg gcgcactgta tgacgaggcg ctgaaatatt
      421 gcatcaactg caaacgttgt gaagtcgcct gcccgtccga tgtgaagatt ggcgatatta
      481 tccagcgcgc gcgggcgaaa tatgacacca cgcgcccgtc gctgcgtaat tttgtgttga
      541 gtcataccga cctgatgggt agcgtttcca cgccgttcgc accaatcgtc aacaccgcta
      601 cctcgctgaa accggtgcgg cagctgcttg atgcggcgtt aaaaatcgat catcgtcgca
      661 cgctaccgaa atactccttc ggcacgttcc gtcgctggta tcgcagcata gcggctcagc
      721 aagcacaata taaagaccag gtcgctttct ttcacggctg cttcgttaac tacaaccatc
      781 cgcagttagg taaagattta attaaagtgc tcaacgcaat gggtaccggt gtacaactgc
      841 tcagcaaaga aaaatgctgc ggcgtaccgc taatcgccaa cggctttacc gataaagcac
      901 gcaaacaggc aattacgaat gtagagtcga tccgcgaagc tgtgggagta aaaggcattc
      961 cggtgattgc cacctcctca acctgtacat ttgccctgcg cgacgaatac ccggaagtgc
     1021 tgaatgtcga caacaaaggc ttgcgcgatc atatcgaact ggcaacccgc tggctgtggc
     1081 gcaagctgga cgaaggcaaa acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc
     1141 acactccgtg ccatatggaa aaaatgggct ggacgctcta caccctggag ctgttgcgta
     1201 acatcccggg gcttgagtta acggtgctgg attcccagtg ctgcggtatt gcgggtactt
     1261 acggttttaa aaaagagaac taccccacct cacaagccat cggcgcacca ctgttccgcc
     1321 agatagaaga aagcggcgca gatctggtgg tcaccgactg cgaaacctgt aaatggcaga
     1381 ttgagatgtc cacaagtctt cgctgcgaac atccgattac gctactggcc caggcgctgg
     1441 cttaaactcc cttctgatgc ccggtaagca tgtggttacc gggcattttt gcgtacacga
     1501 ttccgtgccc aatgtatgcg ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa
     1561 ggctgaaact ggcagatgtc aaaggcctgg gataaccgta atgtcgcgtc atcataaata
     1621 tcaggtgacg gacaaccatg accgaatcaa caacctcctc cccgcatgat gcggtattta
     1681 aaacctttat attcacaccc gaaaccgcac gggattttct cgaaatacat ttaccagaac
     1741 cactgcgcaa gctttgcaac ctgcaaacct tacgcctgga acccactagt tttattgaaa
     1801 aaagtttacg cgcttactac tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg
     1861 gctatatcta ctgcgtgatt gaacatcaaa gctctgcaga aaagaatatg gcttttcggc
     1921 caatgcgcta tgccactgcc gccatgcagc gtcacctaga taaaggctat gacagagttc
     1981 cgctggtggt gccattgctg ttttatcatg gcgaaacatc gccctacccg tactcactta
     2041 actggctgga tgagtctgac gatccgcaac ttgcccggca gttgtacacc gaagcttttc
     2101 cgttggtgga tatcaccatc gtacctgacg atgagatcat gcaacatcgg cgtatagctc
     2161 tgctggaact gattcaaaag catattcgcg accacgattt aatcggcatg gtcgacagga
     2221 tcaccacgct tttggttaga ggcttcacta atgacagcca gctacaaaca ctgtttaatt
     2281 atctgctgca atgcggcgat acctcccgtt tcacccgttt tattcaggag attgccgaac
     2341 gttcaccact acaaaaggag agattaatga ctattgctga acggctacgg caggaagggc
     2401 atcaaattgg ctggcaggaa ggtatgcatg aacaagccat taaaattgcc ttgcgcatgc
     2461 tggagcaggg ctttgaacgt gagattgtgc tggcggcaac ccaactcact gatgctgata
     2521 ttccgaactg ttattaaaag gagagggaga gattgatgac tattgctgaa cggctacggc
     2581 aggaaggaca tcaaattggc tggcaagaag gtaaattagt aggtttgcag caaggtaaat
     2641 tagaaggttt gcaggaaggt atgcatgaac aagccattaa aattgctttg cgcatgctgg
     2701 aacagggcat tgatcgtgac caagtgctcg cggccaccca gctaagcgaa gccgatctgg
     2761 cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc tttgatatca
     2821 ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca gtcgttgatc
     2881 cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg ctccccacgc
     2941 caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt tacccgcagc
     3001 acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc cggcgttatc
     3061 cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat caatcccttc
     3121 gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct gcactaacag
     3181 acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc gcgcagcccg
     3241 tgccacactg gccccaacac cacgctcacc gtagggaggg ttggacaaat tttgccggag
     3301 ggggaatgtg ccggtgcagt aaaccggcac agagtggcga aatcagacgg ataaagtcat
     3361 ggactcaaca acctcaatcc agccatgctc actagcgatg tcctggccgt ttaaccaacg
     3421 gcgtagcata ttcagtgcca tcattgcgca cacttcctga cggatagcca ggctgtagcg
     3481 tgtagtgctg aaacgcacac gcagagcgaa agtgccgtct ggcgtggcta gcgcaaagtt
     3541 gagatgctcg ttctcgaaac ccgaaacagc cagtgccagc ccggcaaaat ggttggcccg
     3601 ccgttctgta atccagtgcg cagtttgcgc cagggtttct tcctgtgaag gaatcacttc
     3661 acacgccagc aatggagcac ctgcgcgaga aagttgcaaa gccaataaac caccggtgaa
     3721 ttgctcgctc aacgtcaggc tgaactggcg gttttgcaat tcgcgactga tctgcgcggg
     3781 cagtccttcg gtgccttcga aaatcacgct ctgtccggca acacgtttaa cgtccagcca
     3841 cagtttttcc atcgcctgtt gctcgcttgc cggtccggtg agtttcagtt cgatgatcgg
     3901 cattgaggag cgatagccca ttgttacgcc cggcggcagt tgtagagtgt ccaggctttg
     3961 tgccagatcg ctttccgaac gaccaaaggt agttaaacgc agacaaaccg gcggctgtgg
     4021 taaagaaaaa cgctcgcgca ggcgcggcag gatttcgtgc tcgaccatca ccttaaattc
     4081 tgacggtacg ccgggagtaa agaacatcag gcaacgatta agctgcacgg caaaaccaca
     4141 ggcggtgcct accgggttat tgataaattc agcactggca ggcagctccg cttgtttacg
     4201 gctgctcggt gccattactc gtccacgttc gtggaaatag cgttccatct ctttgagcca
     4261 ggcttcatgc agcacaaggc cttcaccttt tgctgtcgca gcggcgagtg cgcttaaatc
     4321 atcgctggtc ggtcccagcc cgccgttaac gatcagcaca tcggcgtgct gactacgttc
     4381 gcgaagaatg gtgactaagt catcaaggtt atcccccacc gtattgcggc gagataatgg
     4441 cagcccctga tgaaagaaaa aatcggccag ccaggcagcg ttagtgtcaa cgatttgccc
     4501 gtataacact tcatccccgg tggatagcat ttccactttt aacatggtta tctcctgctt
     4561 ttgttatcga atcactataa cgcaaaggga gataaggcgt agtaagaaac tgaatgccgc
     4621 gccggaaggg atcagaaact ggcgttaacc ccaacataca ggccgtcagc cacagcgttg
     4681 tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc aatactgact
     4741 ggacgcataa tggtgtaacg cgcgccagca ttcgcttcct cataactttg aataccgctg
     4801 gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt gccaattttc
     4861 cactgcaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt cgggttggtg
     4921 tacacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt caaccccagc
     4981 cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct taaagccagg
     5041 cccgtcgatt cagtcccaaa accgacacca atattggtgt attctttacc cgcctggccg
     5101 ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag acctgctagc
     5161 gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc aaagctgcgg
     5221 gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag tcacttattg
     5281 taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt accgatacac
     5341 acaaaaataa taataagtgc agttatcatt gcaaaagaga ttactgtcac tttcacatat
     5401 ttctgagtta ccgtattctc atggtttctt tttatttata aggaataccc ctgtgcgaca
     5461 acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt gcaaaatggc
     5521 cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg tggagtctgg
     5581 cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag aacagctgct
     5641 tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga catatgcaga
     5701 tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt ctgccaaccg
     5761 agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg aagatctggt
     5821 gcattatgat ttaaatgttg ccacccgaaa aacgttacgt ttgaaaggtc ttctttaaca
     5881 atgatgacag ccatcactgg ctgtcaggtt gaaaatttag tggttaacga attccccatc
     5941 cagataaact ttgccttttt ccacatgcat gactaaacca tccagataat caggtttaaa
     6001 tatcgcatca cgcttatttt tagcaatata tgtcaggcaa tgattatggg tgaaaataac
     6061 catattttta tcaggcgctt tgctttgtaa gtccttaatt gcactataaa tctcattacc
     6121 gcactgaaga agtcgtttat ctaccgtcag ctttttaccc gcggaaaacc aggtcgccga
     6181 ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagggatgt cagcgctaaa
     6241 agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg ttttatctga
     6301 caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa cgacaaccgg
     6361 gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac cattgctact
     6421 ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa tgatgaaata
     6481 tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac ctgtagacaa
     6541 gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt agcagttggt
     6601 gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa atttttgcca aatgcaaagc
     6661 ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt tcttaaggtt
     6721 aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac aacactcttt
     6781 atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt cagaattttt
     6841 gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag aggttctcga
     6901 atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt
     6961 gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc atatcacgct
     7021 aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga cctgggtttc
     7081 aaccctcaat atgatttcct tgctgggtgc aacgccggta atggtggatg tcgaccgcga
     7141 tacgctgatg gtcacgcctg aagcgatcga atcagccatt acgccacgca ctaaagccat
     7201 cattccggtg cattatgccg gtgcgccagc agatattgac accattcgcg ccattggcga
     7261 acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg
     7321 gcgacatatt ggcgcaaaag gtaccgctat tttttcattt catgccatta aaaatattac
     7381 ctgtgctgaa ggtggcctga ttgtaactga taatgaaaac cttgcccgcc agctacggat
     7441 gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc
     7501 gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta acgccgcgat
     7561 tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg aaattgccca
     7621 gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc ccgcctggcc
     7681 gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag
     7741 tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt tacatttccg
     7801 cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt taccgaatac
     7861 cgaatggaat agcgaacgca tctgttcgtt gccgctgttc ccggatatga ctaccgccga
     7921 tgccgaccgc gtcatcacag cccttcagca actcgcagga caataagcca tgtttgaaat
     7981 ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc
     8041 ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct
     8101 gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag cctcacaagc
     8161 ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac attcagcgat
     8221 tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg ccgatctcca
     8281 gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt
     8341 agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat
     8401 taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt gtatgctgcg
     8461 cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca gcacctttat
     8521 cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac atcatgccga
     8581 gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga tgtacgacct
     8641 ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcagca gcattattgc
     8701 gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca
     8761 atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca
     8821 gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc
     8881 ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg
     8941 aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc tctgctggct
     9001 gccggttacg aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt
     9061 tatggttcgg tggctcatct ggcggcggaa agagacattc cggtttatgc gccggataac
     9121 gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt gattttctct
     9181 ttttattatc gccatcttat ttgcgacgag atttttcagc tcgctcccgc aggtgcattt
     9241 aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg
     9301 gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg tgccgatgct
     9361 ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc tattacgctg
     9421 catcataaat tgtgccatgc cgcgcgccag ctactggaac agacattacc cgccattaaa
     9481 cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga
     9541 acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca caacatggta
     9601 cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc
     9661 gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct
     9721 gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg
     9781 ggcgacggca ttactatgca gggctcgcaa ttagcgcaga tgctgggcct ggtgcaaggt
     9841 tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc
     9901 ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat
     9961 gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa tcatccacat
    10021 tttcactttg tcgaaggcga tatcagtatt cattccgaat ggattgagta tcacgtcaaa
    10081 aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac
    10141 ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtt
    10201 aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat
    10261 aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg
    10321 atttattcgg tgtcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag
    10381 ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccacgact ggataacctt
    10441 aatgcagcgc gaattggcag ctcccgcgct attacgcaac tcattctcaa tctggtagaa
    10501 ggttcaccaa ttaagctaat tgatggcgga aaacaaaaac gctgctttac tgatatccgg
    10561 gacggtatcg aggcgttgta ccgcattatc gaaaatgcgg gaaatcgctg cgacggcgaa
    10621 attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg
    10681 ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc
    10741 gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg
    10801 agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat gcaggaaacc
    10861 atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga
    10921 ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc
    10981 gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc
    11041 cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc
    11101 tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc
    11161 caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct aaacatcacg
    11221 aagtcggtct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc
    11281 ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag attatcggtc
    11341 aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa
    11401 aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg ttccgtccac
    11461 tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg
    11521 aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc aaccgcatcc
    11581 tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc
    11641 agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt
    11701 taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg
    11761 gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg
    11821 ttaccttatc ggcatcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg
    11881 tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc
    11941 cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac ccattgccgg
    12001 atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc
    12061 aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt
    12121 atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt
    12181 ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc tggtggcggg
    12241 aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt
    12301 tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc
    12361 cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt
    12421 tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctcccct ggggactggc
    12481 gatagcgcag cgggagcctg acttgtggca ctattttttc tgggttgagc atattcaacg
    12541 ctttgcacta gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat
    12601 tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag gctggaaaaa
    12661 ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt
    12721 ctccgtcgct aaaggcaaat tgcccaccta tattctttct tgctttgcac ctctggcaat
    12781 gctgttggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc tgcggattaa
    12841 tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg tgatctcccc
    12901 ctggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc
    12961 ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa
    13021 gacctggtct ttcgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat
    13081 tcctgacaga gtcatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc
    13141 acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg
    13201 gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct
    13261 taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa
    13321 tcaacatcgt caggagggga ttattacact cgtgctttcg gttgaccgcg atgaagatat
    13381 caacagtctc gccattccgc ccgcagatgc catcgttcgt caggagcgtc tggtgctgat
    13441 tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc
    13501 gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat
    13561 atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt gctctggctg
    13621 ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct gaattttgtc
    13681 tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt
    13741 ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga
    13801 tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg
    13861 caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg ttagcttttg
    13921 gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt
    13981 ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt
    14041 atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga
    14101 aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga
    14161 caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac tcatcggggc
    14221 tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta atacaataaa
    14281 gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt
    14341 cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt
    14401 gtttattgca gcacgatttt ttgtgccagc cattccattg cattatcctg tttgctaaga
    14461 gttttcacat caatttacag attaacagat aaactcttta tcaacggcaa tggaatggca
    14521 gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt tgcacccact cttttagcgc
    14581 ctgacgtgaa attttaatac cgccgttttt cagctccggc ggtaaagtca gccagcgcac
    14641 cggttgttga aaacgagcca gcttatcttt cacccattcg ccaagatcaa cggtttgctg
    14701 gtcatactcc accaccgcta ccggccgatg accaaactcc ttgtcggcaa cggaaacgat
    14761 aaacacctgc aaaaccgcag gatgtgcagc aataacacgc tcgacttctt ccggctgaat
    14821 accctcaccg ccactgaaga acagattgtc taaacgtccg acaatggtca gcttgccatt
    14881 atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg tcattaacca gtgaaaccag
    14941 ttgcccgtta cgccagtaac cttctgccat actggcagcc cgcagccaca cttcattatt
    15001 aacgattttc acttcccgac ccggcagcgg caaaccaacg tctgccagga cgtcggcttc
    15061 tttcgcacat accgtggagg caaactcggt cagaccatag ccgcaaaagc aacgaatccc
    15121 ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc gcgccgccaa gtaacaccgc
    15181 tttcagggaa acggaactgc ggttaaccag caaacgccag agttgtgttg gcaccagtga
    15241 agcgtgagta cagcctgcca gcatttgctc caatggctgt ttatcacgca ccgtcatccg
    15301 cgcaccagcg tataaccagc gccacataat tccctgaccg gagacgtgaa acagcggtaa
    15361 agagagcaac cagtcatcgt gatcgccaaa cggaatcagc gataacacac cttgcgcact
    15421 ggcaagatgg gcctgataag tatggacagc ggctttcggc aaaccggtag aacccgaggt
    15481 caacgtcatt gagcacagac gcgtcggctg ccacgcagcg gcatgtacgc cttcaaccag
    15541 ctgaatgtgc agcgacgtta atgccggaaa cgtgttttcc ccatccggca ccagcgcaaa
    15601 ttgcagcgtc agattgggca gcaattcttc aagcaacggt tgcggcagct gagggttcac
    15661 gggcaacacc cgcgccccgc attgcagtaa cgccagccag gcgagcagcg tttgcggcgt
    15721 attccacgcc cgcaacatca cgccgctgcc ctcaaccaca ccctgcgccg caaagcccgt
    15781 agctaactca tcgacgcgag cacaaagctc gcgccagttg agttgctcgt cattaagacg
    15841 taaggcgatg gcttctcccc gcacttgccg ccagtgacgc cacggccagt cagagaagat
    15901 cataacaact gctccagtac atcagcgtcc actaacggca gtgggctccc cggccagcga
    15961 cgaacctgct gcgcctgcat cagatccagc gtgtccagtc ccggaatggt gtctggcgtt
    16021 aaccaggcag caatccgcgc cagttgcgtt agacctaagc tcgactcaat ggaagaactg
    16081 atcaccgccg tcagccccag cgcatgcgcc gcctgtacct gctcgcgtac tttttccaga
    16141 ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc cctcttcagc cacaaaggca
    16201 aaatccggct cgcgcaggct ttcatcccag gcaatggcaa tgccggtttc acgggcaaac
    16261 gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa acgcgatgcg gtggcgataa
    16321 tccgggttaa catatttggc aaactgctga cctttcagcg gtgtccaggc gcgatttgcg
    16381 tcaagacgca aatgcagatc cggaattgcc tccagcaaca gattcaccac catgccgtcg
    16441 cgcaccgctt cgtacaatcc cactttgacc ttcgccactt tctcgcccgg catatcggca
    16501 agtttgagga tcagatcgtc cggatcgcca ttacacagcg gtgccgcacg gtagttggct
    16561 gcttgcggca acgtatctgt cagttctgcc aatgcacagc ttacgccaaa ggccacggaa
    16621 ggcatctgcg gtagctcgca atcgcctgcc agccagttat ttacccaggc aagcagcacg
    16681 ctttgcgcct cttcccagtt ttcctggctg aagcccggca gtggggagat ctccccccac
    16741 ccttcgcgct cgccctcacg caggcaaacg tacagcccgt cgcgggtttt taaccgcctg
    16801 tcgcgcagaa gcacccccgc gtccatgggg atctgccagc ggtatacctg cgcgctacgc
    16861 attacggatt ccgtttaaat ttgctgaaat caggctgacg tttctggttg aaagcgttgc
    16921 gaccttcctg accttcttcc gtcatgtaga acagcatggt ggcgttgccc gccagctcct
    16981 gcagccccgc ctgcccgtca cagtcggcgt tcagcgcagc tttcaggcag cgcagcgcca
    17041 tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt ttctttttcc agatccgcca
    17101 gcggtaccac ggtgttgaca aggcccatat ccagcgcctg ttttgcgtcg tactgacggc
    17161 acaggaacca gatttctcgc gcttttttct gcccgacgat acgagccatg taggaagcgc
    17221 cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg accgaagatg gcattatctg
    17281 ctgcgatagt caggtcgcac atcatgtgca gaacgtgacc accgccgatg gagtagccag
    17341 ccaccatcgc gacaaccggt ttcggacagg tacggatctg acgctggaag tccagcacgt
    17401 tcaggtgatg tacgccggaa tcatctttat agccgccgta atcaccacgc actttctggt
    17461 caccaccgga gcagaacgct ttatcgcctg cgccagtcag aatgatcacg ccgatattgt
    17521 cgtcataacg cgcatctgcc agtgcctgga tcatctcttt taccgtcaga ggacggaagg
    17581 cattgcgcac ctgcggacga ttaatagtga tttttgcgat accgtcggtg gatttttcat
    17641 aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc aaccggtgcg taaggcattg
    17701 cttcatcagg ataaatcata ttgtgtcctt tagtcaaaaa cgcaagatct gcgccagact
    17761 tgcgattacg ccagcgggat tttcccgatg cgcgttatgt ccggcgcgag gaatgacatg
    17821 gcagtcggca gccagttccg ccgctagggc gcggaatttg ctgtcacgtt caccacataa
    17881 ataataaaac gcaaatgtgc gggcgctaag gttagcacgt aaatcaggct ggacggcgag
    17941 agaagtcgcc tccagcatgg cggcaagggt tgcgccatta ttgttgctgc gcagcgccac
    18001 cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca ggctgttgat accagtcggc
    18061 aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc cattggcgat cggaacgctg
    18121 acgttccgca cgttgttcag cattttgcag ccccggatgc ccgccttcga taataacccc
    18181 acaaagcccc gccagcccct ggcaagccgc catcatcgcc acccgtccgc caagcgagta
    18241 ccccaccagc cagaagtcaa ggatgttgta actaaccaag gttttacgca gtaagtcggt
    18301 gacatcatca aatccatcga cgctaatcgc cgccgaacca ccatgacctg ggagatcaac
    18361 atacaaccgt gagtagtcgg caaacgcctc gcccacttct tgccattcgt ggcaatcgcc
    18421 ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt tttccgtgtt ttgcctgcgc
    18481 gtgcaggatc ataaatggct tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg
    18541 gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat
    18601 gccgtttcaa gttcctgcca gttttgcgga caatgatatt tcagctcgaa catcgcggcg
    18661 gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac gctcacgctc gctttgcggc
    18721 gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca ccacaattaa taccagcggc
    18781 gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt aaagtgcgga gagatcgccc
    18841 acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt
    18901 ccgtcgatac cgctggcccc tcggttgctg tacaccgggt aacctgccgg aagttgcgaa
    18961 agcgcatcaa tcagacgtac caccaggctg ttaccaacaa acaattgccc ctgttcaggc
    19021 agatagtcgc tgatgcgatg cgccagttgc gcttcgccaa acgcatcacg gcgggcaata
    19081 accgcctgca ttgcctgttc cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt
    19141 tctgccggat gcagctccag ccagtcggca atattgccaa ttaagcgacg tccgcgatgg
    19201 tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc agtactcttc tggttcacag
    19261 cttgcctgcc attgcaggag ccgtttcccc gtcaggctgc ttcccagttg caccacaatt
    19321 tgtgcctgct gcagctcgct ggtcgctttg gcattgccta accaaagatc ggcacacggc
    19381 agcggctgcc cggtttgtga cagcacatcg ccaatcagcg gccagccaag agtttgcgcc
    19441 cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc
    19501 ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc
    19561 agccacggtt tgtcgtcctg ccaccagtca cccagacgct gttgccagct aagcccggta
    19621 tcgtccattt cgccatacag cggttcagca aacgggcagt tgatatggac tccccccgca
    19681 tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg
    19741 gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg cgaacattcc cggctggcga
    19801 attgcctgat tcgcgccgca gtcaattagc tccggcgggc gatcggcggt taagagaatc
    19861 agtttttctc cggttaaccc ggcttcaatc agtgccggat agagatttgc caccgccgtg
    19921 ccggaggtca caatcaccgc caccggctgc ttgctgactt tcgccagccc cagcgccaga
    19981 tgccccaacc cacgctcatc gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc
    20041 gctaacgtta acggtgtaga acgcgagcct ggggcgatac agatgtgtct gacgccgtga
    20101 cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt taaatgcgct tactgacatg
    20161 agtttgtccg gtatcaatat tgcggctaag tataaggagc tacaaaaata gattattgat
    20221 atgaatcggt aatgatgcga ctcattacta ttccatttgt aataaagtac gcagccctgc
    20281 cgctttgttg tcgatttcct gccactcttg ctcggggtcg gaaccacgga caatgcccgc
    20341 gccagcatat aatcgcacga cattgccgct aatttttgct gagcgcaggg aaacgcagaa
    20401 ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg taccattctc gggtgaacgg
    20461 ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt
    20521 tggctgcaac tgatgtaaac agatcacatc atccgctttg ttgagtgaag tccagataca
    20581 gcggcgaaga tgctgcactt tacgcagacg cagtacctgc ggcggtaaaa catccagcgt
    20641 ctgggtatcg gcctgtaatc gttggcagat atcttccacc accagcatgt tctcgcgctg
    20701 gtttttatca tccgccatca gccactctcc taactgctgc gcctgcttat catcaggatg
    20761 atttgctact gttcccgcca gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa
    20821 ccgttccggt gaagagccaa gaaaagcatt ttcgccatca aaggccatgt aaaaatggta
    20881 gcaattcaga ttcagtcgac gactggcagc catcatcgcc gccgcgttga ccggacttgc
    20941 gaaatgcagg tcagttgccc gagcgagcac cactttgtcg agctcacctt cggcgatggt
    21001 tttcgttgcc agttcgatta attgcgtcca gcccgttttg tccggccagt gttgttctcg
    21061 cgtggtggtt aaatgtaacc caggcaaggg cttgatactc accagtgtgg cgataaattc
    21121 ttttgcctga atcgcatcgt gctgaaggga gctttcgctg aatagcgtca accgcagcgt
    21181 ggctttaccg ccacagcgtc gccattccag gcggggtaaa agtaaattgc cctgcgacgg
    21241 gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt tccgggtgct ggcgaagaaa
    21301 acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg cccaggacgg cagcttcttc
    21361 atcaccatta cgttgttgcc agtagaattg cgggtacgtt tgctgactgg ccagccagct
    21421 caaggcatca aaagcgtctt tgagagggaa aggaatatcg ataacccgaa tgccgggtgt
    21481 cgccggaatc tcttgcgaca aatggcgcag tagattttcc agcgccgtag taagtgattg
    21541 cacgcggacc tctccctgtt aaaagcctca cattatacgg ggtactacaa aaaaaatgca
    21601 gtaccccggt gtagggaggt tttaacggcg tgccagcaac agtcctagta ccagcccgac
    21661 ggccgcgccc acaccaattc cttgccaggg tttttcgtgg acgtagtcat cagcacgata
    21721 aacagcctgc ttcgcccgat agtaataact gtctgaagcc tggctaaccc gttttttcac
    21781 atcatccagc gctttttctg cacgcgcttt cagctcaaca tatttctgat cggcgggctc
    21841 gccagaggag cggagcacct cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat
    21901 acgtgtatca ccaaactgat tagacattct cgttctccat ttgcgtaaaa cctgtcacta
    21961 actatagaca agagttgttg attacgcctg aattacctcg cgtgccatcc caatgtgtgg
    22021 gataccatct tcttcataga cctccgtcac agggataaaa ccgaaactct ggtagaagtt
    22081 ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca ggccagtgat gcgtacatgt
    22141 ttcgagtgtt ttactcatca gctgctgccc cactttttca ccacgcaacg cttcactgac
    22201 aatcacccga cctataacga caggctcaag atcatcatca cttttcagaa tcctcgcata
    22261 cgccaccagt tcatcatttt tccaaccgag aatatggcga ttatcccccg tcaaatcatc
    22321 accatcgata tcctgataag ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg
    22381 taataaggca taaagctggg aaaccgataa ttcagagtga tgcagatctt gccattcaat
    22441 cataatgctc tccttttaga tgtatgcctt atactagccg gaatattttt tgaaacgtga
    22501 tgaactcatg gaattaattt ttttaggtac ttcagccggt gtgccaaccc gcacgcgcaa
    22561 tgtcacggca atattgctta acctgcaaca tccgacccag agcggacttt ggttgtttga
    22621 ctgcggtgaa ggcacccagc atcagctact gcataccgcc tttaaccctg gaaaactgga
    22681 caagattttt atcagtcacc ttcatggcga tcatcttttt ggtttacccg gcttgctgtg
    22741 cagtcgttct atgtcaggca ttatccaacc cttaacgatt tatggtccgc aaggtatccg
    22801 tgaatttgtg gaaaccgcgc agcggattag cggctcatgg accgattatc cgctggaaat
    22861 tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc
    22921 gctggaacac ccactggaat gttatggcta tcgtattgaa gaacatgata aaccgggtgc
    22981 attaaatgcc caggcattaa aagctgctgg cgtgccgcct ggcccactgt ttcaggaatt
    23041 aaaagcgggc aaaacaatca cgctggaaga tggaaggcag attaacggcg cagattactt
    23101 agctgctcca gtgccaggta aagcgctcgc tattttcggc gataccggcc cctgcgatgc
    23161 cgcacttgac ctggctaaag gtgtcgatgt catggtgcac gaagcgacgc tggatataac
    23221 catggaagcc aaagccaata gtcgcggcca tagctctaca cgccaggctg cgacactagc
    23281 ccgtgaggct ggagtcggca agctaatcat tacccacgtc agctcgcgct atgatgacaa
    23341 aggttgtcaa cacctgttac gtgaatgcag gtcaattttc ccggcgactg aactggcgaa
    23401 tgatttcacc gtgtttaacg tttaactctg cttattatta aaaacagggt gaaacttgcc
    23461 ctgttatagc aacccgcgct acatatatcc acccacacag gcatattgag cgatttttga
    23521 tatttattca tctccttacg cactatattc agagcaataa gttgcctctt ttcatcttga
    23581 ttcagcacaa cctttgcaac taaaaggagc ccatgatggt tacagttgtc agcaattatt
    23641 gtcaattatc tcaaacccaa ctcagtcaga catttgcaga aaaatttact gtgaccgagg
    23701 aattactgca gtctttaaaa aaaacagcgt tatccggaga tgaagaaagt attgagttac
    23761 tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa agctgaagat attctttacc
    23821 atattgttag aaacccaaca aatgagaccc tatcgattat ccgacttatt aaaaatgcct
    23881 gtttaaaatt atataatttg gcacataccg caaccaactc ccaccttaaa ccaactggtc
    23941 cggataattc agatgttctc ctgttcaaaa agctattctc cccttcgaaa ttaatgacaa
    24001 ttatcggtaa tgaaattcct cttatatctg aaaaacagtc gctttcaaag gtgcttttaa
    24061 atgatgagaa taatgaactg agtgatggta caaacttctg ggataaaaat cgtcaattaa
    24121 ccacagatga aatagcttgc tatcttcaga agatcgccgc taatgcaaaa aatactcaag
    24181 tcaattatcc tactggtctc tacgtcccct actccaccag aactcacctg gaagacgctc
    24241 tcaatgaaaa tattaagagc gatccatcat ggccgaatga agtccagtta ttccccataa
    24301 atactggcgg acactggata ttagtttcgc tacagaaaat agtaaataaa aaaaataata
    24361 aactacaaat aaaatgcgtc atattcaact cattgcgtgc actaggccat gataaagaaa
    24421 attcacttaa gcgtgtcatt aatagtttta attctgaact catgggagaa atgtcgaata
    24481 ataatataaa agttcattta aatgaaccag agataatatt tttacatgcc gatcttcagc
    24541 aatacttaag ccaaagttgc ggtgcgtttg tgtgcatggc agcccaggaa gtgattgaac
    24601 aaagggaaag caattctgac agcgccccct atacgttatt aaaaaactat gctgacagat
    24661 ttaaaaaata ttcagcagaa gagcagtacg aaattgattt tcaacatcga ctggtaaaca
    24721 gaaattgtta tttagataaa tatggcgatg cacgaatcaa tgcttcttat acacaattag
    24781 aaataaaaca ctcacaaccc aaaaatagag catccggcaa aagagtgagt taacatcatt
    24841 gttaatcaat tcgaccagtc agtaaatatg ctgactggtc gaacataatt tgctaaaaag
    24901 tcatcactga ctgatgtcag tcccaccatc tgccagttca atctacgggt atttccagcg
    24961 tatcgcggca cgcgattgct ggtctgttga tgtagtggat tgtagttgct gacttttctt
    25021 accctcagat ttctgcccca cttgtcaatt caatctgcca ataaataaca taattatatt
    25081 tttattcctc acagaaattc ctttttaaaa atagcgaacc cactgtaaaa aacacttttt
    25141 gtatacctct accaagcgta ataaaataaa cgctcataat tcattataaa taataatgaa
    25201 ataacgcctt ttattgataa tttacatatg aaaaaatttt tccttttgtt atattatttc
    25261 tgttttcact ccctctgatt atttttacca accctgggtc actgcattat caccaacgcc
    25321 ccgacacgta agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca
    25381 tccacgtaat gccggcaata ttgataatct gaataggtcc gcagagtata taaaagagtt
    25441 ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc tattacgact
    25501 ctgcgcctgg aagccagaat tacccttacc cggcaatgtc ctggctttat cccgatcagg
    25561 gagactttat tgctgtggtc ggcagaatgc aggatatcaa cgccgttcgt caggtaaaag
    25621 cggcattgtt gtcatcccgg gatttatctg tttattctat gaatgcccca gggtttattc
    25681 ccggtattga tttttctgat caccttaatt attggcaaca cgatattccc gccgtaatga
    25741 ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat accgcagaca
    25801 gattgaatta tcagaaaatg gctcaggtgg tagatggtgt tataaccttg ttatacaaca
    25861 gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc
    25921 cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga
    25981 ttggctattt tgcagaaatt gactcagaaa agattaacca actgcttgag tctactgaaa
    26041 aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg gatattgata
    26101 aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat cccgcgaaga
    26161 atgatccgct gagtcgtgcc gtgcttgatg aacacagcct cgaagatggc atagatggct
    26221 tcctgggatt gacctggaat caggaattag ccgcgacaat cgaccgactg gaatcactgg
    26281 atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg gagatttatc
    26341 cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcctcgatc atgcttgata
    26401 tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat gcgctgacgg
    26461 ttattgttgg gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt cggcggcttt
    26521 ctgacttaca aactaacagt ttacatcagc ggcattgcta accgcacaat actaatcagc
    26581 ggttgtggcc atacacccag caccagtacc aacagtgcag aaatcagcac cacgataccg
    26641 cccgcgctgt actgccagtt tgacggtgca tcgcgacccg gttgttccgg tgcgtgcagg
    26701 tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc gacaaccacg
    26761 gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttaccg
    26821 ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt catcactgcc
    26881 gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg
    26941 ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga
    27001 taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccggtttg cagcgcaatc
    27061 agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag acgtttgatg
    27121 ttggtctggc tcaacgccat caggttaccg aagatgatgg aggcaaaggc gataatcgcc
    27181 agtactacgc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag acgcatcacc
    27241 acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg
    27301 ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg
    27361 ccaacaatca tcatgccgaa acctgccagc aacagcggct cgttgagcat accgtcgccg
    27421 aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacac cagcgccata
    27481 ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat actggcttcc
    27541 agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt
    27601 tcaataccga ggaacagaga cgccagatgg tttgcattcg ccagcaggat cccgcccagc
    27661 gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc ttcaagccac
    27721 gggtagtcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg
    27781 gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg gccaacaaac
    27841 cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga
    27901 ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac gatcagcaac
    27961 ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta
    28021 gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga
    28081 atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac
    28141 agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg
    28201 aagtaagcgc gatgcaacat cgccagcgaa taaacagatg caaagaccag cccaaaggta
    28261 gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg
    28321 acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag cgacagtgct
    28381 ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata
    28441 cgttcataaa gctgaccgca gagaataaac agacccgccg ccgacaagcc gtgcgcaatc
    28501 atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagattgc aatcagcacg
    28561 aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgagcgaag
    28621 gccatccacg caccgtagaa gatgccgata acacccagcc acatagcgat tggcgcgaac
    28681 tctgccgacg cgttcgggaa cagcggcagg gagaaacgca acaaaccgta agcggcggtt
    28741 ttcagcaaga tccccgcgag gtccacggaa ccggcggtcg gagcctggga gtgcgcatcc
    28801 ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat gaagaagccc
    28861 agcatcaaca gatattccac accactggac attggcgtat tcagcagctc ttcatagttg
    28921 aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcgatcaac
    28981 atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccaccgt gatacgcgtt
    29041 ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc
    29101 atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg
    29161 cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac
    29221 gaacatagta ccgccagcac accgagcaga ccggtcagca cgaccatcag cagcgacaga
    29281 ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat gtcgaattca
    29341 gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt
    29401 tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg tggcaccttg
    29461 acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat taatattagc
    29521 cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca
    29581 acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat gcgccgcgaa
    29641 ggcagcttat ccgacctacg ttcttatatg cccaggggca atcccacaat ccttaactca
    29701 acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata
    29761 ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc gggaaagaac
    29821 cgccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag
    29881 gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca
    29941 ggtactcagc aggcggcccg gcgcactgtt ggcgatggag gtcaccagag tacgtttacc
    30001 cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc
    30061 cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc cctgcagcgg
    30121 cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct
    30181 gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa
    30241 aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg ccaccatcag
    30301 attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc
    30361 agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga gataaaccag
    30421 cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag
    30481 aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg
    30541 gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct
    30601 catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg caaaaccggc
    30661 cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg gcgtcatcag
    30721 gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg ttgcggcgtg
    30781 gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg tctgcaacgg
    30841 caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat
    30901 gttattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt
    30961 gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac ccacacgggt
    31021 cacgacgaac gctttcattg ctgctgcgcc attcttcgga tcggtgtaat agaaaccgat
    31081 cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttatc
    31141 ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga agaagcgaga
    31201 gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga aacccacacc
    31261 agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt
    31321 aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct cgccgttagc
    31381 gaagaaatca acgccgataa aggcggttac ctgcgccgcc aggcccacag agccaacgcc
    31441 gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc
    31501 aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa
    31561 tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag gccgatactc
    31621 gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctgaccc
    31681 cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca
    31741 atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag aacgaataag
    31801 attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt
    31861 tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt
    31921 ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt
    31981 aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct
    32041 gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg
    32101 ccgggccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc
    32161 ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga
    32221 taatttccag cgcaccggcg aagtaagcgc ctagtgagaa gaacaccccg gaaatcgcca
    32281 gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca
    32341 aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc
    32401 ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc
    32461 tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt atttgcccgg
    32521 accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt attcccccat
    32581 ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca
    32641 gaaaatgcag cgtgagaagt taatgcggaa aaattccgga taccagcgac cgtctttggt
    32701 ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcacaga ggttacaggc
    32761 tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg
    32821 cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag
    32881 gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt cttttaaggt
    32941 catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag
    33001 ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc
    33061 ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcagc
    33121 gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcacc
    33181 atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag accgaacttc
    33241 ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca
    33301 aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa accaaagaat
    33361 tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa
    33421 ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagggtc
    33481 tgcgcagaag cacgcatcgc acccagcagc gagtatttgt tgttacttga ccagcccgca
    33541 aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat cccgatgttc
    33601 aggtcggcaa ccacccaacc cggactgact ggcacaattg caaaggccag cagcagcgag
    33661 gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag
    33721 tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc gccccagcca
    33781 acacggttag gtccataacg gttctggaac agacccagca ggcgacgttc gccaaagctc
    33841 atgaatgccc cacaggtgac aaccaccagc aggatcccca ccgctttgag gatggtcagc
    33901 aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa
    33961 gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaag cccacctgcc
    34021 ctgccgtcag tccttcggcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa
    34081 aggagacgcg tgtacctgcg ttcacaccca acttcgcggc atccgctggg ttgagtttga
    34141 tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc
    34201 caaacaggtg gtagtacggc gcgatacgcc atttcccgtc ctgcggctgg aagcgtgccg
    34261 gtacgctggt gaagtaatcc agaccgtttt cgctggtttc aaacagacgc acgcccggat
    34321 cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc tgcggggagt
    34381 tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcggtcggc tggttgttac
    34441 cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc
    34501 tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct
    34561 gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacccgcc agttccggga
    34621 ttttcgccac aacagcgtca attacatggt cgagctgcgt ccagtccact tcacggctca
    34681 gcaaggtgct gtgcagcgag tgcagccagc gccagctttc cagcatgaca gttttgctgt
    34741 cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca ttattaatta
    34801 ccgtaccgtc gctttcagca aagctggcag cggaaagcac cagatgggcg ttttccatga
    34861 tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc gcagcattca
    34921 cgcgggtagc agaagcgtaa cgatgcagat cgttttccaa caccaccacc gcgtcggcgc
    34981 gtccggtttc cagttcggtt aacgcttctt caagcgaacc gccacccata atgcccagcc
    35041 ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg cgacctttca
    35101 gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg ttcgtcccgg
    35161 agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg attttgtttt
    35221 gcagctcagg ttcgataccg tcaaccgctg gtgcggagtt atccagcgca tgggcgatgg
    35281 caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat
    35341 ccagacgagt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt
    35401 tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgcagccatt tcgcgcgctt
    35461 tacctttcac cgcctgacgc actgccagtg cgacgcgcgc gccggtctgg gtaacgtctt
    35521 cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga gtataaatgc
    35581 cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac
    35641 cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt tccacgctgg
    35701 cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct gccgcgccct
    35761 gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt actggctgac
    35821 gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt
    35881 agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg taacgttcac
    35941 cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca aactgcatat
    36001 cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa
    36061 tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt
    36121 agacgttgtc gtgcgcgcca taaacgccca gatctgtacc gtcagcgtaa tctttgtagt
    36181 aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg aatggcccca
    36241 aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca
    36301 tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg
    36361 ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct tcttcgtcgt
    36421 caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac
    36481 gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc
    36541 ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca
    36601 gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta
    36661 gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg ttaccagcgc
    36721 tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg
    36781 attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca
    36841 ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt
    36901 gtttcgatat cgcccggctg gccttcgcca cgctccagcg cacgcagaat tttcacgctc
    36961 cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc gaaaaactct
    37021 tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc
    37081 gtacccagac ggctgcccgc tttaccgata ctttcgaatt ccatcggcag atcaaggtgc
    37141 gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa tttcagacca
    37201 tcacgcatac caccggcgta atcttcgagg atctcgcgtg cggtggtgcc gaacggcagt
    37261 tcccacagtc ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct
    37321 ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta
    37381 cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga ggttgccggg
    37441 aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat taacgctgtt
    37501 tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcgaaacct
    37561 gttcccataa tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat agcgcggcgc
    37621 agatttactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc
    37681 agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg ctccatcaac
    37741 aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag gtaacggatg
    37801 ttcatggatt cgtctttcgg catcaggctc cacttcaggc cagtcgaaaa gcccgcgccg
    37861 ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg agacagcccg
    37921 gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc cagccacact
    37981 ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg
    38041 tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag
    38101 tgtcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca
    38161 gcagcgtaaa gcggccatca aatgtcgttt gccctggttt gatgttcagc tttttctcga
    38221 gcgccgcctg aataccctga tacccgttga tatgacagac cacgctgtcg caataacgga
    38281 tcacatggcg accaaccggc tggcggaaga tctgactgta gaacgttgcc acaccttcga
    38341 cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc
    38401 agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt
    38461 agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg
    38521 tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat
    38581 actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg ccggaatttg
    38641 ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg tgctaccgtc
    38701 gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt
    38761 ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg
    38821 ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc
    38881 ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca gctcttccac
    38941 tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga tttcaaagtc
    39001 gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc
    39061 acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac
    39121 gccctgggta cgacctttca gaatggtatt ttgcagcgcc gctttctcgt aagacgccag
    39181 acgtttcggc atccagtcga ggaactcacg cagcagacga tcccagccgc gcggcaggtc
    39241 gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc
    39301 caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc
    39361 gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga acagttcgga
    39421 gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac
    39481 cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac ggtcggtata
    39541 cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac ggtggtggta
    39601 gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa
    39661 agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt cctcgttttc
    39721 ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc
    39781 ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt
    39841 acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc
    39901 gtcgaaagta atgccaaaca gatcccaggt ttcacgctca taccagttag cgttcgggaa
    39961 cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcagcatgat
    40021 gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa
    40081 cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta
    40141 aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca
    40201 cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt
    40261 gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg
    40321 cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca
    40381 gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc
    40441 aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg
    40501 ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag
    40561 cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc
    40621 aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat cgacgccctg
    40681 cacaacggaa taaatatcgt acataccacc agagttggca caggccccca ttgagataac
    40741 ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa
    40801 gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc
    40861 gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca
    40921 ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt
    40981 gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc
    41041 ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata
    41101 atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt
    41161 catacactcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata
    41221 aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc
    41281 gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac
    41341 gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga
    41401 atcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa
    41461 ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa agatagcgaa
    41521 tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa
    41581 agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg
    41641 aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct
    41701 tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca
    41761 taatatcaac accattagca tttaatgccc tttcacccca gatccttgac gactccagga
    41821 taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt
    41881 tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac
    41941 gcttttgtta ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg
    42001 tagcaggtga tttttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt
    42061 gaaaaataaa cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa
    42121 gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta
    42181 tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg
    42241 agcagacatt gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac
    42301 ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag
    42361 cggcggtaag ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg
    42421 ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc
    42481 gacataagcc agtcgccatg gaatatctgc tttattaagc gtcgccagca ccatatcgcg
    42541 aaacggactt ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta
    42601 ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgctga
    42661 aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata
    42721 ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt
    42781 taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc
    42841 ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct
    42901 ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc
    42961 gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg
    43021 agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa
    43081 tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta
    43141 tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc
    43201 catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat
    43261 atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca
    43321 gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat
    43381 gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg
    43441 tcatacgcga acacgtacaa acagcaatgg tgaagcggca tcaagagcag ggatccgtca
    43501 tttatgcgag cattttaccc caaaaccttt tatttataag ggtcattgcg aattatctga
    43561 tgcaaagtta tgttatgtta tgttatgtta tgttatgtta tgttaggcaa agtaatcttc
    43621 tattattaat aagcacatca aaaccttttt gaatattaaa taataattaa ttagcatcat
    43681 cctcattcat taattccgct taacaatagt ttcacacctt atccccattg cgacacctcg
    43741 gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag
    43801 tgaattctta aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttgc
    43861 aattaagaat tagaacaatt cactatataa cattacatgt aaagcatata cacctcatta
    43921 ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat
    43981 tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg
    44041 cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag
    44101 caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct
    44161 ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc cattcggttt
    44221 tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta
    44281 ttgcgattcc aaagggcttt actccgcgcg taaagccatc atgcagcact accaggctcg
    44341 tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat
    44401 cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga
    44461 ttacccactc tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg
    44521 cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg
    44581 tacgcgtggg attgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga
    44641 gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga
    44701 aatttatgac aaaattctct acgacgatgc tgagcatcac tcaattgcgc cgctggcacc
    44761 tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg
    44821 tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct
    44881 ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac
    44941 cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga
    45001 gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc
    45061 tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcatgacga
    45121 tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc
    45181 attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat
    45241 cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta
    45301 atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc tgatgatgtc
    45361 atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa
    45421 ctcattaacc gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt
    45481 ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa atttggcggt
    45541 aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc
    45601 accggcgatc tccctactcc ggtgaaatac ttcaatgcgc aaatcgctca ggagtacaag
    45661 gctattgaaa aaatcgctca gcagaaactg gtcgatatgg ttccggaaga actgcgggat
    45721 atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa
    45781 caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat
    45841 gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag
    45901 atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc
    45961 caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg
    46021 cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca
    46081 tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg
    46141 gacctaaaac cagggtgtta acaggtgaag acaccggtgt cataaaggcg gcggatgctg
    46201 ccatcgccac gaccatcgca aatggataag gcgacactcc catcgttttg gcagcagcca
    46261 gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaacagc ccaataaccg
    46321 ccgacaagac aaacaaacac cccagcatca tatgtggccc ataaccgccg ccaatatcca
    46381 tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca
    46441 tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat
    46501 ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa
    46561 tttcatttgt cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct
    46621 ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc
    46681 ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc
    46741 ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg cgcttgagcc
    46801 ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca
    46861 gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa
    46921 aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat
    46981 cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc
    47041 cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag
    47101 cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt ggcgatccgg
    47161 ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa
    47221 aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac
    47281 gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc
    47341 taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg
    47401 gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt aacggcatca
    47461 tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga
    47521 tagcgacaac gctggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca
    47581 aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt cccattacgg
    47641 tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa
    47701 cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg cttaatgcaa
    47761 acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga
    47821 cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt
    47881 gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa
    47941 tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat
    48001 ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc
    48061 gttaaccgca atgacatggc aacccgccgc taggccagaa agcacgccag cgggagcatc
    48121 ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta acagatacgc
    48181 atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct ctggtgcggg
    48241 aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc
    48301 ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgccccag aaagcgcggt
    48361 aataccttcg gtttccgtgg cctcgatttg ctccagacgc gtaaactcgg cggcaatatc
    48421 agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt taccgtgaat
    48481 gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc tccacgcccg
    48541 ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca
    48601 ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg ctcaaatggt
    48661 actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg
    48721 tctgggcgtt aaaaccatga gtcccagcgt caaaatgggt atagcgccct tccacattaa
    48781 ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg
    48841 tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg
    48901 catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa
    48961 actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc
    49021 gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac tgattagaca
    49081 aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact ctactacggt
    49141 gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa
    49201 tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc
    49261 tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg
    49321 cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga acgcttgccc
    49381 agccaccaca atccctgcat gggtaaactt aaggcgaaca gtgcagtggc aactgccggc
    49441 ccaagcagac cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata
    49501 aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac cggggcaagg
    49561 cgtttttcca gtggccacgt ctttgagtaa tgctgtccgc ggcgaaacaa gctaaaaaaa
    49621 ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata taaagattca
    49681 aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg tcttccatac
    49741 ccactatcag gtatccttta gcagcctgaa ggcctaaata gtacatattc attgagtcgt
    49801 caaattcata tacattatgc cattggctga aaattacgca aaatggcata gactcaagat
    49861 atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt
    49921 catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt
    49981 acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt
    50041 gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac
    50101 ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt
    50161 gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa
    50221 ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag
    50281 tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga tgcagcttct
    50341 tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc
    50401 ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca gactatgccg
    50461 gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg catccgtcgt
    50521 tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac
    50581 aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct
    50641 gctatccgca acggtaaatg cgttgacacc tctatgggtc tgaccccgct ggaaggtctg
    50701 gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc
    50761 ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt
    50821 ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac
    50881 gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg tgcctacact
    50941 gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg tgaaaatgcc
    51001 gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat
    51061 gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt
    51121 cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg
    51181 actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg
    51241 gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct accggaacca
    51301 gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc
    51361 gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga
    51421 ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg ctgaaaatga
    51481 gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcgtcg
    51541 caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt ctggtcccga
    51601 cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg ctgaatgcgg
    51661 aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa gagcgtatcg
    51721 aactgacccg caacagcttc ggtggtgcca aaaacaccaa catcaccggc gttatcgtta
    51781 acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg tccgagattt
    51841 tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg caagaatcca
    51901 gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga
    51961 tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc
    52021 gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag cacttccgtg
    52081 ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc gcttgcctgg
    52141 cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg
    52201 cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga
    52261 acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa gttccggttg
    52321 acgatcacga acgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact
    52381 ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg ccagcgttcc
    52441 gttatcagct gactgaactg gcgcgcaaag cgggcaaacg tatcgttctg ccggaaggtg
    52501 acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc gcaacttgcg
    52561 tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt gtagaactag
    52621 gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt ggtcgtctgg
    52681 tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca
    52741 acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg gtttccggtg
    52801 ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg
    52861 gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg
    52921 gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc gcgattcagt
    52981 ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca
    53041 ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc
    53101 aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg
    53161 ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct accgtgttca
    53221 tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt tctgccgacc
    53281 tgatctccat cgggccgatg ctgcagggta tgcgcaaacc ggttaacgac ctgtcccgtg
    53341 gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag tctgcacagc
    53401 agcagtaatc tcgtcatcat ccacagcttt gcgctgcgga tatctgaacc ggaaataatc
    53461 actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta tcttgcttaa
    53521 ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt tgttttgtgc
    53581 aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga agatgggcaa
    53641 tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagtctt gccacctggg
    53701 tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa
    53761 aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa gcaggcgaac
    53821 ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc ctgatgaact
    53881 tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc atcatcatgt
    53941 ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg
    54001 gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt cctgcgctgt
    54061 ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg
    54121 ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc accgtcctgg
    54181 tgacttatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg
    54241 ttgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg
    54301 tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc tcacgagtga
    54361 aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg
    54421 cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgacag
    54481 ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt ccagaaattg
    54541 ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt ttccgcctta
    54601 acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga atgatgatcg
    54661 cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggttggt aatggtgaag
    54721 caggtaatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc attagcggtc
    54781 tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggctgtattt aatttcttcg
    54841 tgacgtccgg ttctggtcag gcggcattaa ctatgccgtt actggcaccg cttggcgatc
    54901 tggtcggtgt taaccgtcag gttaccgtgc tggcgttcca gtttggtgat ggcttcagcc
    54961 acatcattta cccaacctca gcttcgttaa tggcaacgct cggtgtgtgc cgggtggact
    55021 tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt atgtccagcg
    55081 tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcaattgc
    55141 ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca ttgcgcttca
    55201 tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt
    55261 cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg aagggaccgt
    55321 gagagacgca actgaacaac gcgccccaga cacggcaatt tttatcttcg aaatagaact
    55381 gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg
    55441 cggattccag cagttgctca tcggcctgaa ctacaccgcc tgcggtcgca tctaacatgc
    55501 cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat
    55561 gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt tcccggctgg
    55621 cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac
    55681 gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt
    55741 tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga
    55801 ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa
    55861 tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc actggcagat
    55921 gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtagattt tccgggccaa
    55981 aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg
    56041 cggttatcgg gaaatgcagc atcatttgat ccacttcgct gtcgcagttg ccacgcacag
    56101 cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct
    56161 ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc agccactggg
    56221 caccgctttg gacaaacaac tccacaacac gttccgtcgc cggtaacgac ccatgaatgt
    56281 ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact
    56341 ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa acgctggaca
    56401 gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac ctcatcgcca
    56461 tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca
    56521 aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag tttctctgca
    56581 ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac aacatccgtc
    56641 ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca
    56701 cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg cgcaaatcga
    56761 tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc gatttgtaat
    56821 aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg
    56881 tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc cacccaggcg
    56941 gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc gggtttactc
    57001 attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt ttgtcactgt
    57061 cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt tcagatgatt
    57121 aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat ggtcacaaaa
    57181 ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta gacctgggga
    57241 aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa attccggcaa
    57301 ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag tctggtgcca
    57361 ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg
    57421 ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg cttgggcaaa
    57481 atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa cgttatcagg
    57541 ttgaaactca gcgtctttac catgtactaa acaagcggct ggaaaactcg ccctggctgg
    57601 gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat gcctggactc
    57661 gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag cggatccgtt
    57721 cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag cgttcggata
    57781 gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg gagaacctgc
    57841 aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta cgtttatcgg
    57901 aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga cgatccacta
    57961 ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt
    58021 aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg aaaaattaac
    58081 tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg aagtggagat
    58141 cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa gctggcagcg
    58201 ttaatcgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac gggattaatt
    58261 ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaattacggt agtgacgcgt
    58321 aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca agggcttgcc
    58381 gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga accgattgct
    58441 gataaacgct ggactcacaa gcaaaaagag cgtctctgcc aaagccactg gaatatcacg
    58501 caaaaactgg tcgatttgat taatgccagc gacacgccgc cgtcggtact catttccggc
    58561 tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg
    58621 cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg ccgggcgcaa
    58681 agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc ggacggcggt
    58741 attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat tggttccggt
    58801 cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct ctggctgctg
    58861 gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccggtacg caatgaacaa
    58921 tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt ccccacaacc
    58981 gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca acgcgcgctg
    59041 cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga agaggcgctg
    59101 gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact ccctgtgtta
    59161 caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac catcataact
    59221 aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc ttcttcgtgt
    59281 ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt
    59341 tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg ggcatcccct
    59401 gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt ccctgtaact
    59461 agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat atgtttctga
    59521 attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg caccacggta
    59581 atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg ctcggcaaat
    59641 tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata aaacagcatc
    59701 gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat tgccgcaatg
    59761 gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg ctctatcact
    59821 acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc ggaataatat
    59881 tgccgcaggt cttcatcaat aaaactgttt ggttctagtt taagcgtcgt taaatcacac
    59941 agtttgcgca gcggcgcggg aagatgaata ttaataaaat tccgcgcggt gtctggatgg
    60001 cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg
    60061 ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca cccaatgcag
    60121 caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac tgcaactttt
    60181 acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca gcgttagcca
    60241 ccggagagga aattatttca gcgatccctt aaggaactgt tgcagacgag ggctttgcgg
    60301 gttgccaaat aactgctccg gcgcgccctc ttcttctatt ttcccctgat ggaggaaaat
    60361 gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca ccatggtttt
    60421 cccctcttct gccagttgct gcataatacg taacacttcg cctaccagtt caggatcgag
    60481 cgccgaggta ggttcatcaa acagtaaaac ttctggttcc atcgccagcg cccgcgcgat
    60541 agaaacacgc tgttgctgac cgccggaaag atgcaccgga tatttcccct gcgcacgttc
    60601 gtctatcccg actttcgcca gatacttcac cgcccgctcg cacgcttcct gcttgctcag
    60661 gcccaacacc tgaatcggcg cttccatgac gttttccaac accgtcatat ggctccagag
    60721 attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc
    60781 ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc cattgaccac
    60841 gatcgatcct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg tacttttccc
    60901 cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca gtgagacccc
    60961 tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta atttattctc
    61021 ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag cgtttttccg
    61081 ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc gcggcaatgc
    61141 cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt ttcagcagat
    61201 ccggcacagt ggccgtaaac gccagcgcag tagagtgcag catcaggatc acttcgttgc
    61261 tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga tacattttaa
    61321 aggtcgagaa gccataggct ctggcggctt caatttcccc atgcggaacc gaacggattg
    61381 ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac
    61441 agttcaggcc actgcggaag aaagcgttaa ggaattcggt tcccttaaca atctcaagcg
    61501 tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg cgaaaaatat
    61561 aggtaaataa ccagattgga aactggatgt atttattact ggagacacga ccaatcgcca
    61621 gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac agagtgatcg
    61681 ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga
    61741 tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa ggaacagcag
    61801 cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga tggcgaaata
    61861 gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat cttccaggcc
    61921 gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc caatgcctgg
    61981 cagggcgtaa cgcatcatcg ccggaaacat gatcctccga aacacttgcc cgcgagtaaa
    62041 accgaacgcc gtcgccgcct ctatatgccc tttcggcact gccataaacg cgccacggaa
    62101 cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag cgaccatcgg
    62161 atcgatatca atctgcccga cgcccatcgc ctccgtcacc atgtttagcg caatctgcaa
    62221 accatagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca gcgtggtgta
    62281 cccttcgaaa atcagcccca aaagccgatt ttgcgagagc ttaccgccag cgccaattaa
    62341 accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga cgagcgcacc
    62401 ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa
    62461 ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt caccgatggc
    62521 gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc gtaagtaccg
    62581 tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt atcttcttta
    62641 cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga cggaccaccg
    62701 aatttgtaat ctttaccgac gggttgtttg aggaaacctt cgctggcagc gacctcatcc
    62761 tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc ctgcccctga
    62821 tacgagacga tttcaatgcc ttttggtgcc cagtgttcat taccgaacgt ctcctgggtg
    62881 gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca
    62941 gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt gaaggcaatt
    63001 tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat cttcttcgct
    63061 tttaaggacg ggattaacgc atccagcggg ttttcgacaa acgtacattg cgtattgatg
    63121 cgcttgcaca actccttcgc cagctcgata tcgaaaccaa ccagttcgcc ttgtgaattt
    63181 tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg cggaattgca
    63241 gcaagtgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag caccagtttt
    63301 ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt tgttacactt
    63361 atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac gtattatttt
    63421 tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt
    63481 gcaccatgat gatcattcca tcaggtacag cttcccagcg acgtatcagt caccgtagac
    63541 attaaagtcg aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc
    63601 aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc ctacaccggt
    63661 gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga aatctttacc
    63721 agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcttgtaacg cagcatccag
    63781 acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca ccacatccac
    63841 gcctttacta cgccaggtct cgttagcgta agcttcctgg gttgatccct gcagcacacc
    63901 aacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt tggcagcaat
    63961 caaacgagaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct gacgtttatc
    64021 ggtaatggaa agcgacgaaa taatagcgtc gatttttttc gctttcagtg aggggagcag
    64081 cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc acatctcgtt
    64141 accgagatcg atatcaaagc caacaaaatc acctttagca tctttcgacg agaacggcgc
    64201 ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc tggaagccgc
    64261 tgcggagaga ccgactaata aagacagagc gagaatcgac ttcttcatac ataaccctca
    64321 agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca
    64381 ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt aataagacgt
    64441 tgcattattg tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat
    64501 tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag
    64561 ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga agggttattg
    64621 caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg gattgcgggc
    64681 gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc gcctgagtca
    64741 ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc aacgggcgac
    64801 gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag ctatggacaa
    64861 tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagcgtc tggaaagaac
    64921 cggaagagat gctggcggaa atatcgcgcg catcgagcgt gacatcagct aatgcctgca
    64981 cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc tggctcatca
    65041 ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc acgccataaa
    65101 tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt tgccctgtca
    65161 gacttgcagg cagactttgc agtatttcgg cgggagttgc aagtcagggt gccagaccgg
    65221 caccctcagc gaaggcatca tccttcgtta tgcatttcga gattttccac ttcgttctga
    65281 cgttgcactg ctttggcgtc atcattacgt aacgtatcga ggaaatcgag gtagccctga
    65341 tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg ctggatatcc
    65401 ggattttcag cgcgaacggc gtcgatcaga tcgttcagat cctggaaaat caacccgtca
    65461 gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg
    65521 ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc agaagcgagg
    65581 tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca
    65641 cggacgatgg agtcgtcgac cagcaggacg tttttatcgc ggaactcggc gcggttggca
    65701 ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat aaaggtgcgg
    65761 ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat acgagcaatt
    65821 tccagcgcga tatcacacga ggtttctggg atcgggatca ccacgtcgat atccagatct
    65881 tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa
    65941 acggaaattt tgtcgataaa cgagtccggg cgggcaaagt atacatactc aaacaggcac
    66001 ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc ttcagtgatg
    66061 taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct
    66121 acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg ttttcccagt
    66181 accagcggac gaatcccgtt tggatcgcgg aaagcaacca taccgtggcc gataatcatc
    66241 gccacacagg catacgcgcc gcggattaag cggtttgtgg cagcaatggc agcgaaaata
    66301 ttgtcggctt ccagcgggta gtggcggaag ttgtccagct cgctggcgaa gatattaagc
    66361 agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta
    66421 cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc atacggggag
    66481 ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca
    66541 tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc
    66601 accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt gatgatgccg
    66661 gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga ctggttaacc
    66721 ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg ttaagccaca
    66781 tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata atcaaaaaac
    66841 catctgatga taaaactgaa ttgcgggatc agctgtgatt tgctccagtc ttcacttttc
    66901 gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa cacaccgcgc
    66961 aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt tttctccacc
    67021 aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat aaacagtacc
    67081 gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca gactgacagg
    67141 taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag cgataacgct
    67201 tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac cgcgattatg
    67261 gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc ctgttcgggg
    67321 cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg catcaataaa
    67381 aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg catcgtgcaa
    67441 tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg gttctaacac tgcccaagcc
    67501 tgataagacg cgccagcgtc gcatcaggca agactgtgtt aattcggcgt atagcccatt
    67561 accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt cagcttatct
    67621 ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg cgtggatggc
    67681 gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt cactttatcg
    67741 gcatttttca gcgcacccag ttgcacaaca taggctttac ccgttggcgc tgctttttct
    67801 tccacgaccg gcttcggttc tggcttcggc gcaggtggtg cttcaacctt tggtttaggc
    67861 ggctccaccg gtttcggctt cggtggggcg accggtgcag gttccggttc aaactcggtg
    67921 ttattggctg caatagtggc cggatcgagc gacggtgctg cggcatcacc tgcccgcacc
    67981 tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc tggcatcata
    68041 tcaggctcat cacgatcgcc cgctttcggc accagcggga tagccgcgaa ctcatcctga
    68101 taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag cgccaccagc
    68161 acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat
    68221 cacttccatg acatgtgcga ccgtgtggaa agaaccacac accagcacgg tgtcttccgc
    68281 tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt
    68341 acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca
    68401 ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat ctttatcatg
    68461 tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca tacgcccggt
    68521 gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct
    68581 cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc
    68641 actgacttcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg
    68701 gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg cccagtcatg
    68761 atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac ctttttcctg
    68821 cgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg gtttttcgct
    68881 gcggaagatg cctgctttct cgcgaccaat actttcgcga tctggaccca gccagtcggt
    68941 atgatccagc gcaatactgg ttactaccgc ggcatcggca tcgacaatat tggttgcgtc
    69001 cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct gcttgaacag
    69061 ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga
    69121 ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcccggc cctgcacacg
    69181 tacgcgctcg gtataacgca ccagatgagg cgaactgtag acgcccactt tgtaccctgc
    69241 cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg tacccgcaac
    69301 ggtaaacaca aatggcgctg gtttcaggac gccaagacgc gccgcgacct ggctcacgcg
    69361 ctcaaggccg agctcgatag ttttactgtg caggttttcc agataagaaa gccacgaagc
    69421 cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg attcgttacg
    69481 gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc aggcctcagg
    69541 ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag gattcggcgc
    69601 tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt ccggacgacg
    69661 gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga atctaggcgg
    69721 cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga ttaacgcttt
    69781 aggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt
    69841 cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc
    69901 agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga
    69961 ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc tgctcaacgg cacgcacgaa
    70021 acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga atgccgcagc
    70081 gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt tttcgccggt
    70141 ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa acttcagctc
    70201 atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta acaggctatg
    70261 caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga cctcaagatt
    70321 acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag tccacacccc
    70381 ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt caatccagct
    70441 cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg gggacgcata
    70501 atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa cagcccgaaa
    70561 ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc gcgtgccgca
    70621 gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa tagaaaccac aataatcccg
    70681 acgatcagca gcttaaggtt atcctgaacc atcgggattg taccgaagaa atagcctgcg
    70741 taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt tataagcagc gaaatgacgg
    70801 tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa
    70861 cgggcgagaa taatcgtttt gccgccatgt ttctcataaa actgatgggt tttgtcgaga
    70921 taactgcgac ggaaaatttt cgaattggga ttactgaata acttttcacc gaacaaccgc
    70981 ccgatagtgt aattgaccgc gtcacccaca atcgcggcga tcagcatcag cactaccatc
    71041 atatggacgt tgagatcgtt ggtttccagt gatgccaacg ctccggcaac aaacagcaat
    71101 gaatcacccg gtaaaaacgg cattaccacc agaccggttt cacagaacaa aattaagaac
    71161 aaaatggcat aaacccagac gccgtactcc gcgactagct ctgccagatg cacgtcaatg
    71221 tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc cttgtactcg
    71281 tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc attattctcg
    71341 ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc ggtcagggta
    71401 atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc
    71461 ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac cgacttccat
    71521 caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt tgatatcaac
    71581 caccacataa ggaccgtgac gcgtgacatt aatatgcata acgttgcgcc acggggttcg
    71641 ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca agcattgcgc
    71701 agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag
    71761 taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag ccgtggcgct
    71821 aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga tgtcaccagg
    71881 taaattcgca tttacgccca aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa
    71941 atgcaccact tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt
    72001 gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca cactgcggac
    72061 ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc ccagcgcaat
    72121 tttataaact ggcagttgtt gctggtcgga cattagtaca gatactcctg caccagtttc
    72181 tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag
    72241 agctggactt gctccggcat accgtagtca ttacgcacgc agccaacaga aagatgcggc
    72301 gtacccgaag catcccctac ctgagtcggg aattcgttct cttcagagag cacaatatct
    72361 tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag tgcttcaaag
    72421 ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc
    72481 agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg
    72541 ctaccttcgc tatccggcaa caacggcagc atattgaacg ccagttgacg accgaagaaa
    72601 tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc taacgcatcg
    72661 accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat acgcgacaaa
    72721 ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag actgtctggt
    72781 acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac ttccggcacc
    72841 accagcggta cgtcgggttc gagagcaaac agtccgctgc tgtcgatcac caggcaacct
    72901 gagttggtcg cttcttcaac ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc
    72961 agctgcgcct gcgtccagtc gaattcagcg gcatcctgca cggtgattgt tttaccacca
    73021 aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc cccaaccggg
    73081 aactgacgtt cagccagcgt ttcaagcagg gcttcgccca tagcgccagt tgcgcccagg
    73141 acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa agctccctgc
    73201 caatgttaca gcagagagca tgaagaagag attaacgtgc cggatgatga acggcgttaa
    73261 aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca tacagagatg
    73321 accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc ggtatcccgg
    73381 cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc accagccttt
    73441 tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg gccaaactca ggcgcgggca
    73501 gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa cttgctatag gcttcaaaca
    73561 cttgcgtcgt gccacgcgct ttaccctcca aggtatagcc tgcgatatgc ggcgtgccga
    73621 tatccacttt tgtcagcaat tccacattaa gttccggctc gccttcccag acatccagca
    73681 ccacgcttaa cttctggcct tcattcaggc aggtcagcag cgcggtatta tcgacgactg
    73741 cgccacggca ggcgttaatc agaatcgctc cgggcttcag gctacggatc agtttttcat
    73801 ccgccagatg tagcgttttg tacggaccat ctttaaagag tggcgtatgg aaagtcagaa
    73861 tatccgcgcg ctggactaac tcatccagcg agcggaaatc accctcatcc ccacggtcgg
    73921 cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc ttccagtcgc gcctgcaatc
    73981 gacgtccaac gttaccaacg cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat
    74041 cgcgttcggc aagcatcagc agggaggaga aaacatattc caccaccgca atcgcattac
    74101 agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca tcgacatggt
    74161 cggtccccgc agtggcagtt ccaacaaatt taatgggttt tcctgccagc aaagattcat
    74221 tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga gcgacgggga
    74281 ttggacgccc gggaaccgcg atcacctcac ccaaacggct aaataagtcg cgggcataag
    74341 gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac gagagttaac
    74401 cggacaagtg tgccataatc tcgcggccag gcatacttgc gaagatttca ggtataagga
    74461 tacgtaatga tacaacctat ttccggccct cctcttgggc agccaccagg tcagggagat
    74521 aacctaccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac ttcgctggaa
    74581 agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact gtgggcgggt
    74641 atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc
    74701 gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc tgcccgccag
    74761 cttttagcgc aattagggga gtatttacgt ctggggaata atcgtcaggc ggtcacggat
    74821 tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa
    74881 accattctca ccctgttgca ggaagggaag atggttattc cgcaaccaca gcagcgcgag
    74941 gcgaccgacc gtcctttatt accggcggag cacaatgcgc taaaacagct ggtgaccaaa
    75001 cttgcggcgg caacggggga acccagcaaa cagatctggc aatcgatgct ggaactttcc
    75061 ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt gacctggcta
    75121 caggcgcgtc agacgctaag ccagcaaaat acgccgacgc tggaatcact acagatggcg
    75181 ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat ccagcaaaaa
    75241 tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat tcttaatcag
    75301 ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct gcttaatcct
    75361 tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct gtggatactg
    75421 ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt aaccccgacg aaatgacagt
    75481 atcgtgacaa taatacccag caccgcagag atcgccccgg caagaaatac cgaagagtaa
    75541 ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga cgccgagggc gatatcctga
    75601 aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc
    75661 tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc cgcgcccgct
    75721 aatgcgaccc atgcacctgg ggcttgccag agcagcaaca agcccaccgt ttctacaagc
    75781 agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc aaacatgacg
    75841 cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat cgcccatcct
    75901 ttgctggcaa agcagagcga aacgaaagtc ccgataaccg caaaaccaac gccttgtagt
    75961 gccagcccta accctggttt ccagataagc ccgacaacgc tccacagcga tggacgttct
    76021 cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag taagggtaat
    76081 accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc
    76141 ggcgcaccaa cagcgagggc accgtaaatc gccattccgt tccatgacat cactttgcca
    76201 gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag taactggctt
    76261 tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa cggtgcggag
    76321 acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc taacatcccc
    76381 tgaagcgccg aacgttttgc gccatattga tcggccagtc gcccggcgta accacgcgtc
    76441 agcaccgtag ccagaaactg aatcccgacg gcaatgccga ccatggtatt gccatagccc
    76501 agatcatgat gaacaaacag cgggataacc ggcaacggca accctacggt catgtaggtg
    76561 agaaaaaccg caaaagcgat gcggaagagc gaaaaactgg cagaagatcg tgtttcggtt
    76621 tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag tatccccgtc
    76681 tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca
    76741 gaaggaaatc gacaaagcgg gaagtttgcc tagaactggt ggcgattgtc aatgatgtga
    76801 aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg
    76861 tcggatgcga cgctggcgcg tcttatccga cctactccgg cctactgcga attaatcttt
    76921 cagcttgcgc attaccagcg tggcgttggt gccgccgaag ccgaagctgt tagacataac
    76981 agtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc agacccgcag cctgctcgtc
    77041 cagctcttca atgttgatgc tcggggcgat aaagccgtgt tccagcatca gcagagagta
    77101 gatagcttcc tgcacacccg cagcgcccag tgagtgaccg gtcatggctt tggttgcaga
    77161 aatcgccggg ctcttatcgc cgaacacttc acggatagct gccagctctt tcacgtcgcc
    77221 aaccggagtc gaagtaccgt gagagttcag gtaatcgatt ggggtatcaa cgccatgcat
    77281 cgccatctgc atacaacgta ctgcgccttc gccagacgga gcaaccatgt ctgcaccatc
    77341 agaggttgcg ccgtagccaa cgatttcagc atagatgtaa gcaccacgcg ccagcgcgtg
    77401 ttccagctct tcaaccacta ccataccgcc gccgccagcg ataacgaaac cgtcacggtg
    77461 agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg tatttagtag acagcgcacc
    77521 catcgcgtcg aattcgcagg ccatttccca gcacagctct tcgccgccgc cagcaaacac
    77581 gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta ccgatacagt gtgcggaagt
    77641 cgcacacgcg gagctgatgg agtagttaac gccatgaatt ttaaacggcg tggcgagaca
    77701 ggcagaaacg ccggatgcca tcgctttagt gaccacatac gggccaaccg ctttcaggcc
    77761 gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa cgcggggaac cgccgccgga
    77821 acctgcaatc aggccaacgc gcgggttatt ctggtaagct tccggagaga ggcccgcatc
    77881 agcgattgcc tgctccatag aaaggaatgc ataaatggat gcgtcgctca taaagcgcac
    77941 aactttgcgg tcaatgaggc cagtggtatc cagttttatg ttgccccaga cgtggctacg
    78001 catgccggaa tccttcagct cctgagagaa agtgatccct gaacgtcctt cacgcagaga
    78061 tgccaggact tcctgctggt tattaccgat gctggaaaca atgcccaggc cagtaatcac
    78121 tgcacgtttc attcaatacc tctgtaagtc gcacatagag taagtttcga atgcacaata
    78181 gcgtacactt gtacgccgaa caagtccgat cagccattta atagagaaat ttgcgcagcc
    78241 ttacacacat cgctaagatc gagccaccgc ctgtaagacg agtaacttac gtgaaacact
    78301 actccataca acctgccaac ctcgaattta atgctgaggg tacacctgtt tcccgagatt
    78361 ttgacgatgt ctatttttcc aacgataacg ggctggaaga gacgcgttat gtttttctgg
    78421 gaggtaacca attagaggta cgctttcctg agcatccaca tcctctgttt gtggtagcag
    78481 agagcggctt cggcaccgga ttaaacttcc tgacgctatg gcaggcattt gatcagtttc
    78541 gcgaagcgca tccgcaagcg caattacaac gcttacattt cattagtttt gagaaatttc
    78601 ccctcacccg tgcggattta gccttagcgc atcaacactg gccggaactg gctccgtggg
    78661 cagaacaact tcaggcgcag tggccaatgc ccttgcccgg ttgccatcgt ttattgctcg
    78721 atgaaggccg cgtgacgctg gatttatggt ttggcgatat taacgaactg accagccaac
    78781 tggacgattc gctaaatcaa aaagtagatg cctggtttct ggacggcttt gcgccagcta
    78841 aaaacccgga tatgtggacg caaaatctgt ttaacgccat ggcaaggttg gcgcgtccag
    78901 gcggcacgct ggcgacattt acgtctgccg gttttgtccg ccgcggtttg caggaagccg
    78961 gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg ggaaatgctt tgtggggtga
    79021 tggaacagac attaccactc ccctgctcca cgccgtggtt taatcgtacg ggcagcagca
    79081 aacgggaagt agcgattatc ggcggtggta ttgccagcgc gttgttgtcg ctggcgttat
    79141 tacggcgcgg ctggcaggta gcgctttatt gcgcggatga agcccccgcg ctgggtgctt
    79201 ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa acacgatgag gcgctaaacc
    79261 gctttttctc taatgcgttt acttttgctc gtcggtttta cgaccaatta cccgttaaat
    79321 ttgatcatga ctggtgcggc gtcacgcagt taggctggga tgagaaaagc cagcataaaa
    79381 tcgcacagat gttgtcaatg gatttacccg cagaactggc tgtagccgtt gaggcaaatg
    79441 cggttgaaca aattacgggc gttgcgacaa attgcagcgg cattacttat ccgcaaggtg
    79501 gttggctgtg cccagcagaa ctgacccgta atgtgctgga actggcgcaa cagcagggtt
    79561 tgcagattta ttatcaatat cagttacaga atttatcccg taaggatgac tgttggttgt
    79621 tgaattttgc aggagatcag caagcaacac acagcgtagt ggtactggcg aacgggcatc
    79681 aaatcagccg tttcagccaa acgtcgactc tcccggtgta ttcggttgcc gggcaggtca
    79741 gccatattcc gacaacgccg gaactggcag agctgaagca ggtgctgtgc tatgacggtt
    79801 atctcacgcc acaaaatccg gcgaatcaac atcattgtat tggtgccagt tatcatcgcg
    79861 gcagcgaaga tacggcgtac agtgaggacg atcagcagca gaatcgccag cggttgattg
    79921 attgtttccc gcaggcacag tgggcaaaag aggttgatgt cagtgataaa gaggcgcgct
    79981 gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt aggcaatgtt cccgattatg
    80041 aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa agatgaggcg gtaagcgcgc
    80101 cggtttttga cgatctcttt atgtttgcgg ctttaggttc tcgcggtttg tgttctgccc
    80161 cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga accgattccg atggatgcca
    80221 gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg gaaattgttg aagggtaaag
    80281 cggttaaggc gcggtaatct gctctcgcag tgtttgccgg atgcggcgtg aacgccttat
    80341 ccaacctacg tgtgacgtgt aggcctgata agacgcgata agcgtcgcat caggcatggt
    80401 gctccagacg ccgcaactta cagctgtgac gcctgctgaa acaggttttc ccacatatcg
    80461 gtcaccagcg cctggtcacg cggcgacaat tcaccggcac caatggcttt ttccagactc
    80521 tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat caccactttc cagttctgcg
    80581 atggctaatg tcaggtggcc acgcaaatac ccactggcaa acaactcatc atcactggca
    80641 tggtcaacca tgccgtcgat taatgccaga atgcgtgatt caaactccgc gatcatcttc
    80701 tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg cttccggcca cgggaactgt
    80761 tccgccgtaa gttccggcgt gtgataataa ttttgtagtg ctttaatgaa gcgtgccggg
    80821 cgttccggaa tgccatttgt cagataatcc atcacctgcg catgtacccg ccgttgaaac
    80881 accacacgat ccggttcgaa atccccttcc agattgtcgc aactgacgtt aaacggatat
    80941 ccagccgcca cgcagaataa ccagtcgaac gcctgcggct tcacttcaac atcttcaaac
    81001 tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc agtagccgaa atcaaccagc
    81061 tcacggcgcg cttttccggc aatacaccag tgcgaaatct catgaattgc gctggcataa
    81121 aagccatgag caaagacgat tcggttatac ggtacttccg catcagcagg aagatagatc
    81181 ggttcgtcgt cgcctttaat cagacgggta ttaaattcat cggcaaagca gctattaaaa
    81241 atttcaatca actgctcgta gtggtgtgta ctgttcatta gttcatcccc aaccagtgga
    81301 ggatctcctg tccgtggcta tcgtaaagta atttggcact catcaccgcc gagacgataa
    81361 caatcatcgg gcggatcagc ttttgtcctt tgctcaacac caaccgtgaa cccatgcgcg
    81421 cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc ccaaatcact ttgccgccga
    81481 gaataaacag cagcagaccg ccaatgtttg acgttgcgtt gagtaatttg gcgtgagccg
    81541 tggctttggc gaggttgaat ccgcacagca taacgaaggc cagcgcgtaa aacgatccgg
    81601 cagccgggcc aaagaatccg tcataaaaac cgacgcagcc accggcaatt aacgcaaagg
    81661 gtaaaccgta catccggcgc tggcgatctt cttcacccag ctttggcatc agcaagaaat
    81721 agaggccgat acaaatcacc agaatgggca aaatctgccg taagacatca gcctgaacgt
    81781 actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt catggcaata ttgagtttct
    81841 gatcgcttaa actaaccact ttgcggcgaa taaagtagat agtggcggaa atagagccgc
    81901 cgcaggcttg cagtttattg gttgccagcg cgttagcggg agacattccc gcagccatca
    81961 atgccggaat ggtgagtaac ccaccgccac cggcaatcga gtcgataaat cccgccagca
    82021 tggcgacaaa aaagagaact cccagcaaca gcggagaaac cataaacagg ctattaaacg
    82081 tttccattag atcacgtgct catccagtag cgcctggcag gaaggcggca acggaggcgg
    82141 tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt tcaaaccagc tttgcagttc
    82201 tgccccgcaa ccatcgcctg atggcggtaa aggttgatct tcacactcca gactatcggc
    82261 agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg aaccagggtc gcactttgcg
    82321 caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt tgtttaatcg ccggattaac
    82381 aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg atcaagctga aaatttctgg
    82441 cttccacagc gtggagacaa cgtgtttacc gtcgcgggaa actaagtcca gtgcttgcgg
    82501 gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt tgcagaaaga tatcgacatc
    82561 cagtccggtc tggtggctgg catgaccgcc gttgaaacgc ccaccagcgg gcatccccat
    82621 atcgccaatc agcaccgtac ccatgcccag attgctcacc tggctactca gacgctggat
    82681 aaacatcacc agatccgggt gaccgaaata gcgacgctga tcggtacgca tgacctgata
    82741 atgttcggac tgtatcggca acgtgtcagc gccgacaata cagccattag aaaaactgcc
    82801 tatcgattgt gcgctacccg gcacaggttg ggttattttt tgccacggcg tcgctgccag
    82861 gctagcgcta ctggcaagca gagccagcag cgcaatcgcg gttttgttca ttttttacca
    82921 gcgtggaata tcagtcttca catcggcatt ttgcgcccgc tgccgtaaca ggtgatccat
    82981 taaaacgatc gccagcatcg cttcagcgat cggcactgca cggatcccga cacagggatc
    83041 gtgacggcct ttggtgatca tctcaacttc ttcgccaaag cggttaatag tgcgccccgg
    83101 cacggtaatg ctggaggtcg gtttcagcgc catatgagca atgatttgct gcccgctgct
    83161 gataccgccg aaaatgccgc ccgcatggtt gctctggaaa ccctctttgg tgatttcgtc
    83221 gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg tcgccaattt ccacgccttt
    83281 caccgcgttg atgctcatca gcgcatgggc gatgtcggca tccaggcggt caaagaccgg
    83341 ctcgccaagt ccggcgggga caccactggc aacaacggtg actttcgcgc cgatggagtc
    83401 gccctctttt ttcagcgcgc gcatcagttc atctaacgcg tcgattttgt cggggtccgg
    83461 gcagaagaac ggattttgtt caaccagcga ccagtctttg atttccagcg gaatgtcgcc
    83521 catctgggtc aggcagccgc ggatttcaat accaaatttc tcggcgagat attttttggc
    83581 aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg gaagaacgtc caccgccgcg
    83641 ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca gcatggcctg gacggaagac
    83701 gtctttaatt gcgctgtaat cctgagaacg ctggtcggta ttttcgatca acaagccaat
    83761 gctggtgcca gtagtaacgc cttcaaaaac accggagagt attttgacct gatccggctc
    83821 gcggcgctgg gtggtatagc gcgatgtccc agggcgacga cgatcaaggt catgttgcag
    83881 gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg acgatgcagc cgagcgccag
    83941 cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt ccaattgtgt ttccagccat
    84001 cacggctccg ttattgttgt gtttgcgtgt ttacttaatc tttataaatc gcgaaatgtt
    84061 ctcgtgcggc aagaagctgc tctttagtga gcataaacac accatcgccg ccgttatcaa
    84121 actccagcca ggtgaacgga acatcgggat attgttccat aagatgtacc atgctgttgc
    84181 ccacttcaca aatcaacacg ccatcatcag caaggtaatc tgccgcgtta ccgagaatgc
    84241 gacgcgtcag tttcaggccg tcggtgccag atgccaggcc cagttccggc tcgtgacggt
    84301 attcgtttgg caggtcggac atatcttccg cgtcgacata cggcgggtta gtgacaatca
    84361 gatcgtattg cactttcggc aaatcacgga acagatcgga acgaatcgga atgacgttgt
    84421 ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc cagcgcgtcg ggagagatat
    84481 ctaccgcgtc gacttccgct tccgggaagg cataagcaca ggcaatggcg atgcagccgc
    84541 taccggtaca catatctaaa atatgctgcg gttgcttgct gatgagtccg gcaaatttat
    84601 tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac gcgttcatcg acgtaaaatt
    84661 catgaccgca gaaccaggct ttgttggtca ggtaagccac cggaatgcgc tcgttgacgc
    84721 ggcggatcac gcgttcaaca atacggtgtt tttcgctgga ggtcagacgc gcggtgcgca
    84781 tatcttccgg gatatccagc ggcaggtaga gcgatggtaa caccagctgt acggcttcat
    84841 cccacgggtt atcggtgccg tgaccgtacc agatatttgc cgcgctgaag cggcttaccg
    84901 accagcgcaa catgtcctga atggtttgca gctcatttac tgcttcatca acgaaaattt
    84961 tatccacgta ttcctccagg gcatgatcgc aataatttcg gcggctagtt tgccatgaag
    85021 atgacgataa atcagcattc acgcgcggtg agtgaggaaa aatacgttta aaacgatcga
    85081 ttgtgctacg agtcgggtaa actgtaggaa aattagaaat agagacagat aaatgaaaaa
    85141 gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc cagttgatgg cggggactcg
    85201 caagattaag caggacacga ttgtccaccg accgcagcgt aaaaaaatca gcgaagtgcc
    85261 ggtgaaacgc ttgatccagg agcaggctga tgccagccat tatttctccg atgagtttca
    85321 gccgttatta aataccgaag gtccggtgaa atatgttcgc ccggatgtca gccattttga
    85381 ggcgaagaaa ctgcgccgcg gcgattattc accggaattg tttttggatt tacacggtct
    85441 gacgcagctg caggccaagc aggaactggg cgcgttgatt gccgcctgtc gccgcgaaca
    85501 tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat attttgaagc aacaaacacc
    85561 gctgtggctg gcacaacatc cgcatgtgat ggcatttcac caggcaccga aagagtacgg
    85621 cggtgatgcg gctttgttgg tgttgattga agtggaagag tggttgccgc cggagttgcc
    85681 ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct tatccggcct acaaatatgt
    85741 gataattcaa taaattgcaa ttcacttcgt aggcctgata agacgcatca gagcaatact
    85801 acgaacttca gttcattcaa tacatactga aaacgacacg tcgaatcaat cggttgccca
    85861 ctactggtat tcacattcac cacaatatcg tgtagtactt tatccgttac cggatgacga
    85921 gttggcgtaa ccgttaccgt tgatttatca caaccgtaag aggtaaaggt taacaattct
    85981 gataccgcgc ggcagtatgc tcctctgcca tcattatcag aaaaattact gccatcacag
    86041 agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg tcactgttga ccccgtggac
    86101 tgaattcgta acgccttatt atggctgaca taattttcac tccaggagat atcgtatgaa
    86161 gcgaccggta tcgttgggca tattgtgtta tgcactgtag gggccgtata gcttccaaat
    86221 ccgggtaaat ctgtatagga ataaaaggat gcgatccaag ggcaaccata atcatcagat
    86281 gcaacggtaa ttttaaacgt catatatgca caacgaaaaa ttttcgtatc tatgccaggg
    86341 caatttgagt tattaattgt atcaaagtca gtcagtacca aagttggagc tgaacccgac
    86401 ccagaccagg aaataacccg caacgtatcg cctttatacg caaaagaatc atatttccca
    86461 tatgcaacct cctttggcgt tatcaccgta acattgaaaa cactgctcgc tacagcaccg
    86521 gttaccttgt ccatggcata ataaactctc acactgtcaa aactcgcact cataagttta
    86581 tctccagcat aactcttaga tgcgccgaca acactcagaa cgagcagcat taggctcgcg
    86641 aaaattatgg tccatttttt catttcagaa atactcaaag gttgccgtca ctgtggcact
    86701 aaagtctccg gacgttacat ctcgcccgct ctttgcctgt aactaggtat taaaatttag
    86761 gctgttattt ccctgattca gcgggagcgt gacgcctgtt gtattgttga ttggcaccga
    86821 tgtgcctgcc gcagtttcaa taccaatccc agcgccggat gctgatgacg aggagtcaaa
    86881 ggccagaaag ccgggtagag cactatcttc tgttcctgtc aacgttactt tgacattaaa
    86941 caatgtagaa ctatgacaat ctttcaattg aaacaccaat ggcactcgct ctgactgccc
    87001 aaggttcatt aaatcgctgg cggcaatggc aggaaaatgt acttctgcca actggctacc
    87061 attaatcact ggcgtacacg atttactgat taaattgccg gtaaagtgca aattatctcc
    87121 tgctattgtg gttccactgc ccagcaacat caggctacta ataaaaaaca acgtacgctt
    87181 tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct caaagtccgc ctctggcaga
    87241 atgacaccac tcttttttac tggcacagcc gttaacaccg gtaaatcggt ttcattcacc
    87301 acaagtggcg tattgatcgt aaaactctgc cctgcctgtt tgagctgaat gcctaaacca
    87361 gtgatattgc tgcttactgc agcattatca aaatcgcttg gtgtgccact ccagctcaag
    87421 gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg taatggaata cggcacggtt
    87481 tgctcataat tgctgccatt aatgtcatca ataagcacat cctgaaaagt tatttcaatg
    87541 gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta aataaccgtg aaaagagacg
    87601 tttgagagtg ccatcgagca aaagccccac agcaaaataa gcgagatccg tttcattggt
    87661 aatccaccac aattgtggca ctggcgttaa attcagctgc cgctaacgtt gtaccactct
    87721 gtttcaccgg cacagcttcc agaacgggaa ctcccgagga aaaattaaat ggcaaccatg
    87781 agccagaggt taagtcgagt attgtgtgat cactactttt ctgaacccga ataccaaacc
    87841 caggaatacc cgtgctgata actgtttcac cgttaatcac gaccgttgtc gcctgcatct
    87901 gtagacgcag gtcatccatt gccagattgt tacaaacaag cgagtagtca atgggacgtt
    87961 tataatcaac cctattgatc ttattaatga atacattacc aaactcaact gccgcaccat
    88021 ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa attcatactt actgacttac
    88081 tcgctgcaac tgcaaagccg ctaccaacac aacatatcag taacccagtt accgcccaga
    88141 cagaacaaga tcctttcata ctggctctcc cggcctcagc ctttgcggct ctgctcttca
    88201 tcgacctagc aactgttacc gttgcaagtg aaaaagagcg gcatccgagc gccatagtcg
    88261 ttgacatagg tcagtaccgg cacgctaccc atcttcacat tcagcggtgc agaggttttc
    88321 ggcgacatca ctattggcga gaaaccggct gccggattgc catttttctg tgtgctggca
    88381 ttactgaaaa tgacgtaata cgacgtcggg ttattgacgg taaattcctg accgttgcgg
    88441 gttaaagtaa ccttatattg ataggggttt ttcatgctga cgttctccag cgccttcggt
    88501 cgccagaaca acttaatacg cgtttgtagt gctatttgca gcgtgttggc tttgtttgat
    88561 tttggcggga tctcacgcac gttgaagtag aacaggcttt cacgatcggc aggcaatttg
    88621 ttgatatccg gcattccctg cacttttacc tgcccgttca tcattgaatc gatgcgttgc
    88681 accggtggca gaacagttaa cggtgaactg atcttgttac ccttttcatc ttcgatccag
    88741 ctttgggcca gataaggcag tttcggatcg ttattgcgca acgtcacgct gattgatttg
    88801 tcgctttcat taaatatcaa acgggtacga tctggcgtta cagaagcctg ggcagacaaa
    88861 ctggcagcgc taagcagtaa cgctagcgtc gtagcaccca atttggcaga gcaaagtaaa
    88921 tcagacatgg atcattcctt aatcacgtta ttggttagct gaaacagaaa cagggggatt
    88981 ggtgggtgtc acctgttgcg tctgctcctg aatgacaggc tttatgtcat caggaacaac
    89041 cggcgcgacg ttgcctttat gctggcatgg cagtaacaag ccgttaaaca gatcggcagg
    89101 cagcgggtcc ggcaggttga tatcgcaatg cgcaacacca ctccagaaca cactcatgtg
    89161 ttcgccaggt ttcaccccag ccagataaac attgccgtca tcatcgacaa ggcccactgt
    89221 ctgctcgtta tcatttttta cttctgcgcc aaacggagga tggctgccgt cttgtaaacg
    89281 cagcaccgcc atcgcttttt gaccactgat gacggcaaat ttgcggtagc caatcgcccc
    89341 ttcagttagc gtggcttgta ccaccgactg ggttgcttcg gcgttttccg gcagtttgtt
    89401 gaggtcgata tacgcctgat tgcggtaata gttattgacg tcagaaacga cggctttacc
    89461 aaacatattg gtataaacag cagcaccgtt accttctacc ggaacatcgg caacgccatc
    89521 ggcatcaatc aacaagcgtg tcccgcccat attctgggta cggtgaagtg cgccaccgtg
    89581 ggcagtaaac gttgcgccgc cctgtaacga caagcccgca gaggtgtatt gcccttcatg
    89641 gtagttcgca ctgaggtcaa cctgcgccag cgaaccatca tggctgtaat agccgtcaac
    89701 gctggtgtgt ttgtcactgg tgccgacgtt caactgatag tgagtcgcgt catcgacacg
    89761 gctgaaataa ccgacctgac tgctgtccgt cccactgcca tagtaccgtt atagctaacg
    89821 gtactgttgt cgccccacgg catactgagc gaaatgtaca tgcctttgtc ggcctggttg
    89881 tcatactcgt aacggtagcc agtcatcgag atgctgacat tacgaatact gcccatattg
    89941 aaatagtgcg agagcatgat gttgtagttt gtctgctcct cgcgatccca gtaggtatgg
    90001 cgggtatagt tgagataaac cgaaacaccc gcatcgcgga agttctggtt ataagtggcg
    90061 gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc atccagatac
    90121 tcgctcatgg tcataaagtt ctcttccgag aagcgatatc cagcaaaagt aacgcggctg
    90181 ttgagctggt caaagtcttt ggaataactc acacggaagg agttaccgtc cagcgaacct
    90241 ttgccataag cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc aaacgcgacc
    90301 gcgccgaatg tagacaaatc gcgaccgaca ccaagcgccg cagactgata gttttcatct
    90361 cccagtgcgc cgccatataa cgaccagccg ttagcgatcc cccaggaagc ttcagcacca
    90421 gaaaaaaatt caccctcgac atggtgtccc cactcttgcg gacggcccat catgatctta
    90481 tagcgaacct gacctggacg agtgaggtat ggcatcgagg cggtgctgat gtcatattcc
    90541 tgcacctggc cgttctgttc ttcaatgcga atatgcaacg taccggagac agaatcacca
    90601 agatcctgaa tacgaaacgg tccagccgga acctgcgttt cgtaaatcac acgccccatc
    90661 tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg cgcgtagccg
    90721 cgcagattgg gaggcaacat ttgatcgtca gtactgacac tgccaccaac atagttaaaa
    90781 ccatcaaaaa tatcggatct gaggtaatcc tcgcccagcg ccagtttggc ttttaatgat
    90841 ggtaacgccc gcctggcata gtagcgactc cactcccatt ttttttgagt ttcatcgccg
    90901 ctgaattcat cgtcatcatt actgcgagta tgttgatagt tagtctgcca gtcagcacgc
    90961 atacgccacg gccctaggtt aaccccaacc gtcccgttgc cgctgatctc gttactatca
    91021 tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc gacgatcccg
    91081 gagatgccgt catcccaacg tgaaggcgga tcccagtcgg gataagtata ttcgaggtaa
    91141 gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat ttccatgcct
    91201 tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt tttcgccacg
    91261 tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata gcttttgctc
    91321 gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg ctgtttattt
    91381 agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga aaaacgcttc
    91441 agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg aatgtcgtct
    91501 tcagcccaga cactgctata actccctgac attgccagcg caatgcacca cggaagaatc
    91561 cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta ttcttgttcg
    91621 gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg ggggtatgcc ttacaggtaa
    91681 gtaatctgga aggtggtatt tgcttcaaaa ttacccagat ctggcgcatc agctgcgccg
    91741 accagccagg ctttaaagtt cagtgtctgc ttcgctttgc ctttgttggt agaagtgtct
    91801 ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag agagtcaccg
    91861 atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat ggtgtaataa
    91921 ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt ttcctgagta
    91981 tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt gccgttattt
    92041 ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc gtccggaacg
    92101 atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt gttacccgca
    92161 gcgattgttg ccgtagaggt gaacacgccc atcaccattg ccgcagcaat agctgtttta
    92221 acaaacttac tcattatttg tccttaaact ataaataatt aaaaatataa acattgcaat
    92281 acattgattc agtcaatagc caatgtttat acccagccat tgaatacggc atgcgaccca
    92341 aaaagaacag gtcgtagaaa attgtattca ttggtgtggg gccgcatagt gatgtttcca
    92401 cacccctgat ccaatttaat tttactaaag agtaaatgta gatttaatta atatattgac
    92461 agaagaaagg ttatcttaag gacatcctta aaaaatacgg aataagtatt tagaaaaaat
    92521 agaaagtata tcaataatga atatcaacag cttaaaaaac aaacaatgaa catataatgc
    92581 gattggcatt tatccacttt tgacaatata atatataaac actatagcca gaatttaatc
    92641 aagtttcaat tacatcctgc ctgattattc aactctattt tctctattat ctgtattaat
    92701 aaatgacact gagtgtcaaa cagacattat tcatttcaac atgtaacttt gggcaaaatc
    92761 aagatacgcg cagaagaata atttccgaat gaatgtagaa agtgttttat ttatagataa
    92821 ggagagtctt cgatagtagt gaggttgaca gatatccgct gcctaatgag caattatgcc
    92881 caatgaatct acctcattgg gcataatttg gaacacatca gatagctttt gccatcttca
    92941 gattacacgg gctcatctgc cagttgaatg taccgtttcc gctttcatca agcgttacgc
    93001 tggcaatggc cgaggtggta aacataggcg gcgtttcgcc cggacataac tcggcaacca
    93061 gatacccgac taacggtaag tgagagatca ccagtaccga agcaacacct tcattggtca
    93121 acgcctgcaa ataggcactg accagaccaa catcgccgca gggcgttaac tctggcagaa
    93181 cttctgcact ggaaggcagg ttcagacaat cccctacttc ttccagtgtt tgctcggctc
    93241 gcaggaacgg gctcaccaga acgcgttcga tttccacttt ttgacctttc agccagttcg
    93301 ccatcaggcg agattcgtca caaccattag tggtcagagg acgaaccgaa tcactggcgg
    93361 catcgagggc tgcgtcgccg tgacgcatga taaaaacttg catattgcac cgcttttgtt
    93421 aaccagattc gcccgttttc tttcatctga aaccagagaa agaaaacggt ggccggcatt
    93481 gtgccttatc cattcaccga atgaaacgct gttttttacc tcaatggcgt aagtatagtc
    93541 aatatgcgtt tacattttga ccaacactcc gccattcagc gcggattcat atagctttga
    93601 ccttcttatt gcaggtcagt tgcagttgtt ttccaaaaac tttccccacg cgcgcccatc
    93661 tcgaccaaac gctcgcaagg ggtaaaacgg gaaccatact gcgtggccag tcgttgcatt
    93721 attgcaacca cttcgcccgc gccgagagaa tcgatatagc ggaacggtcc accgagaaat
    93781 ggcggaaaac caatgccaaa taccgcacca atatccccgt cacgcacgct acggataacc
    93841 tgctcatcaa cacaacgtac tgcttcattc agcatcaaca tcacacaccg ttcagcaacc
    93901 tgcggtgcgg agagtcgccc ctgcccttgt gcgccaatga gcgggtaaat ggcaggatcg
    93961 acctgttttt tgcttttacg ccctttctga ccataaagat agaaaccccg accatttttt
    94021 ctgcctttgc gatcgtcgtt caaaattgaa gaaacaacat ttgcaggcgc gctaaaacgt
    94081 tctccataag cggcttccag tacaggaata attttagtcc cggtgtcgat tcctacctca
    94141 tccaaaagtt ggattgggcc taccggaaaa ccaaatttca ctagcgcggc atcaatgtgc
    94201 tctacccgtt caccttcggt cagcatgcgg atagcttcat taatgtaagg cgctaagatg
    94261 cgattgacgt aaaacccggc tttgtcacgc acgacaatgg gcgttttgcc ctgttttttc
    94321 gccagtttta ccgtagtggc gatggtttgc gccgatgtac ccgcatgagg aataatctct
    94381 accagcggca ttttttccac cggactgaag aaatgcaggc caataacttg ctcaggtcgc
    94441 gcggcgtgag cggcgatatc accaatcggt aaagatgacg tgttcgaagc aaaaatggta
    94501 tgagcggcgc aattttgctc cacttccgcc accatctgtt gttttaattc gagattttca
    94561 aacaccgctt caataatcag atcgcgatgg gcaaagccgc gatagtccgt cgttccggag
    94621 attaatgcca gctgtttgtc acgttcgctg gctttgagat gacgacggcg aactttgccc
    94681 tccagctgat cccaactgta cttcagcgca tgatttatgc cctgcgggtt gatatcttta
    94741 attctgaccg gaagccccgc tttacaagca gtgacataag caataccgcc gcccatcaag
    94801 ccaccaccta aaattcccac gctgtttaat ggcgcaggcg gtgcatcact accgggatct
    94861 ttcttcacgt ccgtactggc aaaaaagata ctacgcaaag cttgcgattg cggcgtcatc
    94921 gccagttcgc caaaggcgcg agcttcagcg tcatagccgc tgctggtgcc ctgcgctaat
    94981 cccgtttcaa caacctccag gatgcgttct gtcgccggat aattgccttg agttttgtgt
    95041 tctgttttct tgccgaccat tttgaacagc agcgcacgac ctaacggccc cgccagaata
    95101 cgctcgcgta caggtagagg gcgggaagat gggcgatcct tcttcgccag ctcaacagcg
    95161 gcttccagca aaatggagtg cggaacaacg tcatccacca gccccagctt taatgcctgt
    95221 ttcgcccgaa gttgttttcc ggtgagaatc atctctaatg ctgtactgac gcctatcaga
    95281 cgcggtaaac gctgggtgcc gcctgaaccg ggtaacaatc caagttgtac ttcaggcaaa
    95341 ccgagcaccg ttttgggatc gtcagtacaa acgcgaccgt ggcacgccag cgccagctcc
    95401 agcccaccgc ccaggcaagc gccatgaata gccgcgataa ccggaatggg caaagcatga
    95461 atctccgcca tcaactgttg gccctgccgc gccagtgctt ccgcttcttg cgccgttttg
    95521 cagttgccaa tcatgttgat gtctgctccg gcgataaaat tgtccggttt cgcggagata
    95581 aacaccacgc cgcgcaactc tttgttttca cggagttgct taataatggc gcgcacctgc
    95641 gaggcaaatt ccgccttcag ggtattcatt ttctcatccg gtacgtcgat ggtgatgata
    95701 gcaatgttgt ccagacgaac attaagagta aacgctgatg ccatttccat tattccgcct
    95761 ccagaaccat tgccgcgcca agcccaccgg cagcacaggc ggtaactaaa ccaaatccac
    95821 cgccgcgacg gcgaagttca tgcaatgtct gggtaatcat ccgcgcgccg gtcgccgcga
    95881 agggatgtcc gtaagcaatc gaaccgccaa gcacgttaaa tttgctatcg tccacttcgc
    95941 cagtggcatg tgcacgcccc agtacttcac gagcaaaacg ttcactaccc agcaactgaa
    96001 tattcgccag tgtctgagct gcaaaggctt cgtgcatatc gatcaatgtc agttcgctca
    96061 tcgtcaaacc ggcacgttcc agcgccagcg gtgttgacca ggctggaccg agcaacatgt
    96121 cctgccagac atcaatcgca gtaaatgcgt agctgcgcag ataccccagc ggcaccagcc
    96181 ctaattcttt cgcccgggat tcagtcatca ggatcaccgc tgccgcgcca tcggtcagcg
    96241 gcgtactgtt tgccgccgtt accgttccgt gtttgcgatc aaacgccggg cgcagctttg
    96301 cgtaatcggc aagcgaggaa ttaccgcgaa tattgttgtc ttcgacaagc ggttgtttat
    96361 aaggagggat aaaggcagtc atcacctctt ctttgagttt tccgtctgac catgcctgag
    96421 cggcacgctg atgcgaacgg tgcgctaatg catcttgctg ttctcgggtg atgccgtagg
    96481 ttttcgccat ttgttcggcg gtgtcgccca tccgcaagcc agtagaatat tctgctaccg
    96541 caggcggtac gggcattaag tcgcgcaaac gcaggcgaga gaagagtttc aggcgctggc
    96601 tcatggttcg agctttgttg acatcaacca gcacgtgcgc cagtttttta ctgacgccaa
    96661 ttggcaatac ggaagaggaa tctgccccac cggcaatccc cgctcggata gttcccgcca
    96721 tcaggctttc tgcgacgttt gcaaccgcct ggaaactggt agcgcaagcg cggctgacgc
    96781 tgtaagcatc ggtatgcaca ttcattccgg taccgagaac aatttcacgc gcaatgttgg
    96841 gggcttcagg catttgtacg acctgaccaa agaccagctg ttcaatcact tcagcgggga
    96901 tctcgctgcg tgccagcagt tcgcctacca ccatcttccc taaatcaacc gcgggaatgc
    96961 catgaaaagc cgtcgcctgg cgggcaaaag gcgtacgtaa accgctaaca atggcgatac
    97021 gatcgccctg gcgggtaacc agtggtaaaa cctgacccat aacactcccc tgtaaaaaaa
    97081 taaataaagt ggtctgacct gatcatagta ttaaccattt ttttacattt agccaagcgg
    97141 agaaaaagga aagtgggagc tatgacacag tgaagacaga agaaagaaaa gaaaacgccc
    97201 ctgccagcaa ctgacagggg cgtacgtacc cgagaggaat taacgcagac ccagctggaa
    97261 gatcagcatt tcagcttcgc aggcaaaagt gaaatcgata tccaggcgca caccgtcaga
    97321 ctcttcagtg aaagtcgggg agattttgca cggttcggat tccacgctac gggatttttc
    97381 agtcagcgcc gccagcgttt gttctgcttc tgcgcggttt gcaaacacgc ggctgtaaga
    97441 cgcggtgcag tcggagttgt ccataatggt gccaacatcc atacagcagc aaaccggggt
    97501 ttcatcagca ctacatttac tcatcgttga tttcctctgt atgtgcaccc aaggtgccag
    97561 ataaacgttg tggatatttt acgcttccgg aaagtgctgc tccagttgtt aattctgcaa
    97621 aatcggataa gtgaccgaaa tcacacttaa aaatgatcta aaacaaaatt cacccgaatc
    97681 cgcgagtgcg caacctccaa attttgccag ctggatcgcg tttcttagat catatttgaa
    97741 aaaagatgga aacatacttg caacattcta gctggtccga cctatactct cgccactggt
    97801 ctgatttcta agatgtacct cagaccctac acttcgcgct cctgttacag cacgtaacat
    97861 agtttgtata aaaataaatc attgaggtta tggtcatgag ccagaaaacc ctgtttacaa
    97921 agtctgctct cgcagtcgca gtggcactta tctccaccca ggcctggtcg gcaggctttc
    97981 agttaaacga attttcttcc tctggcctgg gccgggctta ttcaggggaa ggcgcaattg
    98041 ccgatgatgc aggtaacgtt agccgtaacc ccgcattgat taccatgttt gaccgcccga
    98101 cattttctgc gggtgcggtt tatattgacc cggatgtgaa tatcagcgga acgtctccgt
    98161 ctggtcgtag cctgcaagcc gataacatcg cgcctacggc atgggttccg aacatgcact
    98221 ttgttgcacc gattaacgac caatttggtt ggggcgcttc tattacctct aactatggtc
    98281 tggcaacaga gtttaatgat acttatgcag gcggctctgt cgggggtaca actgaccttg
    98341 aaaccatgaa cctgaactta agcggtgcgt atcgcttaaa taatgcatgg agctttggtc
    98401 ttggtttcaa cgccatctac gctcgcgcga aaattgaacg tttcgcaggc gatctggggc
    98461 agttggttgc tggtcagatt atgcaatctc ctgccgggca aactcctcaa gggcaagcat
    98521 tggcagctac cgccaacggt atcgacagta ataccaaaat cgctcatctg aacggtaacc
    98581 agtggggctt tggctggaac gccggaatcc tgtatgaact ggataaaaat aaccgctatg
    98641 cactgaccta ccgttctgaa gtgaaaattg acttcaaagg taactacagc agcgatctta
    98701 atcgtgcgtt taataactac ggtttgccaa ttcctaccgc gacaggtggc gcaacgcaat
    98761 cgggttatct gacgctgaac ctgcctgaaa tgtgggaagt gtcaggttat aaccgtgttg
    98821 atccacagtg ggcgattcac tatagcctgg cttacaccag ctggagtcag ttccagcagc
    98881 tgaaagcgac ctcaaccagt ggcgacacgc tgttccagaa acatgaaggc tttaaagatg
    98941 cttaccgcat cgcgttgggt accacttatt actacgatga taactggacc ttccgtaccg
    99001 gtatcgcctt tgatgacagc ccagttccgg ctcagaatcg ttctatctcc attccggacc
    99061 aggaccgttt ctggctgagt gcaggtacga cttacgcatt taataaagat gcttcagtcg
    99121 acgttggtgt ttcttatatg cacggtcaga gcgtgaaaat taacgaaggc ccataccagt
    99181 tcgagtctga aggtaaagcc tggctgttcg gtactaactt taactacgcg ttctgataac
    99241 gcgttcgcct ggataaagtc acctgctatg caggtgactt taactccccc acttcaccga
    99301 aagtagtttc ctcattgctt gcctcctgag ttttgcaaac agcctgatgg cagcttgccc
    99361 tttcaaatca gtaagtggta tccacagaca aacctgaagg gaaatgcata ttttcaagcg
    99421 ttctgctcgt ccttctcaaa gagtttactt ttctgcattt ccaggatact caacccccct
    99481 ggctattgtg cgcttataca ctcaaattaa agataggttc taaataaatg agcgtttttt
    99541 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaacat agaataaaat
    99601 catgctacca tctatttcaa tcaacaatac cagcgcagct tacccagaat ccatcaatga
    99661 aaataacaat gatgaaatta atggattagt acaagagttc aaaaaccttt ttaatggtaa
    99721 ggaaggaata agcacctgta ttaaacatct acttgagctt ataaaaaacg ccatacgagt
    99781 aaacgacgat ccttatagat ttaatattaa taattcctca gttacttata ttgatattgg
    99841 ctccaatgat acagaccata ttactattgg tatcgacaac caagaaccaa tagaattacc
    99901 tgcgaactat aaagacaaag aactcgtccg tactatcatt aatgacaaca tagttgagaa
    99961 gactcatgat atcaataaca aggaaatgat cttcagcgca ttaaaagaaa tatatgatgg
   100021 agatcctggt tttatcttcg ataagatatc acacaaactc agacatacgg taacggaatt
   100081 tgatgagagc gggaaaagcg aaccaacgga cttatttacc tggtacggta aagataaaaa
   100141 aggcgactct ctcgctattg taattaaaaa taaaaacgga aatgattact tatctctcgg
   100201 ttactacgat caggacgact accacattca aagaggaatt cgtattaatg gtgatagtct
   100261 cacccaatat tgtagtgaaa acgccaggag tgcttcagcg tggtttgaaa gcagtaaagc
   100321 tatcatggca gaatcatttg caactggttc cgatcatcag gttgtaaacg agctcaacgg
   100381 ggaaagactg agagaaccta acgaagtttt taaacgttta ggtcgagcaa taagatataa
   100441 ttttcaagtg gacgatgcaa aatttagacg cgataatgta aaagaaataa tttctacttt
   100501 attcgctaac aaagttgacg ttgaccatcc tgaaaataaa tacaaagact ttaaggatct
   100561 cgaagataaa gttgaaaaaa gacttcaaaa ttgccaggcg aaatatcaaa atgaaattaa
   100621 tcaactatct gcactaggtg ttaattttga tgatatttaa aattattttt ccagtttttt
   100681 gatttttcaa cgcctgaata tcggaaatag tcagtgacaa a
//
LOCUS       DADLTI010000002        82358 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLTI010000002 DADLTI010000000
VERSION     DADLTI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.002"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..221)
                     /locus_tag="JN755_000099"
     CDS             complement(<1..221)
                     /locus_tag="JN755_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7379027.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPT"
     gene            complement(292..2406)
                     /gene="fusA"
                     /locus_tag="JN755_000100"
     CDS             complement(292..2406)
                     /gene="fusA"
                     /locus_tag="JN755_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7379028.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2503..2973)
                     /gene="rpsG"
                     /locus_tag="JN755_000101"
     CDS             complement(2503..2973)
                     /gene="rpsG"
                     /locus_tag="JN755_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7379029.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3070..3444)
                     /gene="rpsL"
                     /locus_tag="JN755_000102"
     CDS             complement(3070..3444)
                     /gene="rpsL"
                     /locus_tag="JN755_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7379030.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3570..3857)
                     /gene="tusB"
                     /locus_tag="JN755_000103"
     CDS             complement(3570..3857)
                     /gene="tusB"
                     /locus_tag="JN755_000103"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7379031.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3865..4224)
                     /gene="tusC"
                     /locus_tag="JN755_000104"
     CDS             complement(3865..4224)
                     /gene="tusC"
                     /locus_tag="JN755_000104"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7379032.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4224..4610)
                     /gene="tusD"
                     /locus_tag="JN755_000105"
     CDS             complement(4224..4610)
                     /gene="tusD"
                     /locus_tag="JN755_000105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7379033.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4610..5332)
                     /locus_tag="JN755_000106"
     CDS             complement(4610..5332)
                     /locus_tag="JN755_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7379034.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5499..6311)
                     /gene="fkpA"
                     /locus_tag="JN755_000107"
     CDS             complement(5499..6311)
                     /gene="fkpA"
                     /locus_tag="JN755_000107"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7379035.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6532..6750
                     /locus_tag="JN755_000108"
     CDS             6532..6750
                     /locus_tag="JN755_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7379036.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6799..7389)
                     /gene="slyD"
                     /locus_tag="JN755_000109"
     CDS             complement(6799..7389)
                     /gene="slyD"
                     /locus_tag="JN755_000109"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7379037.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7484..7684)
                     /locus_tag="JN755_000110"
     CDS             complement(7484..7684)
                     /locus_tag="JN755_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7379038.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7694..9499)
                     /gene="kefB"
                     /locus_tag="JN755_000111"
     CDS             complement(7694..9499)
                     /gene="kefB"
                     /locus_tag="JN755_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7379039.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9499..10050)
                     /gene="kefG"
                     /locus_tag="JN755_000112"
     CDS             complement(9499..10050)
                     /gene="kefG"
                     /locus_tag="JN755_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7379040.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10181..12094
                     /locus_tag="JN755_000113"
     CDS             10181..12094
                     /locus_tag="JN755_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7379041.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12094..13116
                     /locus_tag="JN755_000114"
     CDS             12094..13116
                     /locus_tag="JN755_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7379042.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13110..13328
                     /locus_tag="JN755_000115"
     CDS             13110..13328
                     /locus_tag="JN755_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7379043.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13382..14251
                     /locus_tag="JN755_000116"
     CDS             13382..14251
                     /locus_tag="JN755_000116"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7379044.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14306..14710)
                     /locus_tag="JN755_000117"
     CDS             complement(14306..14710)
                     /locus_tag="JN755_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7379045.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15012..15644
                     /gene="crp"
                     /locus_tag="JN755_000118"
     CDS             15012..15644
                     /gene="crp"
                     /locus_tag="JN755_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7379046.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15695..17785
                     /locus_tag="JN755_000119"
     CDS             15695..17785
                     /locus_tag="JN755_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379047.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17852..19072)
                     /gene="argD/dapC"
                     /locus_tag="JN755_000120"
     CDS             complement(17852..19072)
                     /gene="argD/dapC"
                     /locus_tag="JN755_000120"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7379048.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19158..19721)
                     /gene="pabA"
                     /locus_tag="JN755_000121"
     CDS             complement(19158..19721)
                     /gene="pabA"
                     /locus_tag="JN755_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7379049.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19753..20355)
                     /locus_tag="JN755_000122"
     CDS             complement(19753..20355)
                     /locus_tag="JN755_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7379050.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20345..20512)
                     /locus_tag="JN755_000123"
     CDS             complement(20345..20512)
                     /locus_tag="JN755_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7379051.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20617..21189)
                     /gene="ppiA"
                     /locus_tag="JN755_000124"
                     /gene_synonym="rotA"
     CDS             complement(20617..21189)
                     /gene="ppiA"
                     /locus_tag="JN755_000124"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7379052.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21460..22641
                     /gene="tsgA"
                     /locus_tag="JN755_000125"
     CDS             21460..22641
                     /gene="tsgA"
                     /locus_tag="JN755_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7379053.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22903..25446
                     /locus_tag="JN755_000126"
     CDS             22903..25446
                     /locus_tag="JN755_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7379054.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25443..25769
                     /gene="nirD"
                     /locus_tag="JN755_000127"
     CDS             25443..25769
                     /gene="nirD"
                     /locus_tag="JN755_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7379055.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25895..26701
                     /gene="nirC"
                     /locus_tag="JN755_000128"
     CDS             25895..26701
                     /gene="nirC"
                     /locus_tag="JN755_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7379056.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26720..28093
                     /gene="cobA"
                     /locus_tag="JN755_000129"
     CDS             26720..28093
                     /gene="cobA"
                     /locus_tag="JN755_000129"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7379057.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28340..28507
                     /locus_tag="JN755_000130"
     CDS             28340..28507
                     /locus_tag="JN755_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7379058.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28802..30139
                     /gene="frlA"
                     /locus_tag="JN755_000131"
                     /pseudo
     CDS             28802..30139
                     /gene="frlA"
                     /locus_tag="JN755_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30160..31182
                     /gene="frlB"
                     /locus_tag="JN755_000132"
     CDS             30160..31182
                     /gene="frlB"
                     /locus_tag="JN755_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7379059.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31232..32062
                     /gene="frlC"
                     /locus_tag="JN755_000133"
     CDS             31232..32062
                     /gene="frlC"
                     /locus_tag="JN755_000133"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7379060.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32059..32844
                     /gene="frlD"
                     /locus_tag="JN755_000134"
     CDS             32059..32844
                     /gene="frlD"
                     /locus_tag="JN755_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7379061.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32944..33675
                     /gene="phoB"
                     /locus_tag="JN755_000135"
     CDS             32944..33675
                     /gene="phoB"
                     /locus_tag="JN755_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7379062.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33835..34920)
                     /locus_tag="JN755_000136"
     CDS             complement(33835..34920)
                     /locus_tag="JN755_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7379063.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34932..36236)
                     /locus_tag="JN755_000137"
     CDS             complement(34932..36236)
                     /locus_tag="JN755_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379064.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36248..36539)
                     /locus_tag="JN755_000138"
                     /pseudo
     CDS             complement(36248..36539)
                     /locus_tag="JN755_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
     gene            complement(36550..37428)
                     /locus_tag="JN755_000139"
     CDS             complement(36550..37428)
                     /locus_tag="JN755_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7379065.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37425..38651)
                     /locus_tag="JN755_000140"
     CDS             complement(37425..38651)
                     /locus_tag="JN755_000140"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7379066.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38652..39815)
                     /locus_tag="JN755_000141"
     CDS             complement(38652..39815)
                     /locus_tag="JN755_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7379067.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(39899..40261)
                     /locus_tag="JN755_000142"
     CDS             complement(39899..40261)
                     /locus_tag="JN755_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7379068.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40278..41183)
                     /locus_tag="JN755_000143"
     CDS             complement(40278..41183)
                     /locus_tag="JN755_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379069.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41453..42457)
                     /gene="trpS"
                     /locus_tag="JN755_000144"
     CDS             complement(41453..42457)
                     /gene="trpS"
                     /locus_tag="JN755_000144"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7379070.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42450..43208)
                     /gene="gph"
                     /locus_tag="JN755_000145"
     CDS             complement(42450..43208)
                     /gene="gph"
                     /locus_tag="JN755_000145"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7379071.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43201..43878)
                     /gene="rpe"
                     /locus_tag="JN755_000146"
     CDS             complement(43201..43878)
                     /gene="rpe"
                     /locus_tag="JN755_000146"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7379072.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43896..44732)
                     /gene="dam"
                     /locus_tag="JN755_000147"
     CDS             complement(43896..44732)
                     /gene="dam"
                     /locus_tag="JN755_000147"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7379073.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44839..46125)
                     /gene="damX"
                     /locus_tag="JN755_000148"
     CDS             complement(44839..46125)
                     /gene="damX"
                     /locus_tag="JN755_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7379074.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46217..47305)
                     /gene="aroB"
                     /locus_tag="JN755_000149"
     CDS             complement(46217..47305)
                     /gene="aroB"
                     /locus_tag="JN755_000149"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7379075.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47362..47883)
                     /gene="aroK"
                     /locus_tag="JN755_000150"
     CDS             complement(47362..47883)
                     /gene="aroK"
                     /locus_tag="JN755_000150"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7379076.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="JN755_000151"
     CDS             complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="JN755_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7379077.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49434..49838)
                     /gene="hofP"
                     /locus_tag="JN755_000152"
     CDS             complement(49434..49838)
                     /gene="hofP"
                     /locus_tag="JN755_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7379078.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49828..50268)
                     /gene="hofO"
                     /locus_tag="JN755_000153"
     CDS             complement(49828..50268)
                     /gene="hofO"
                     /locus_tag="JN755_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7379079.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50252..50791)
                     /locus_tag="JN755_000154"
     CDS             complement(50252..50791)
                     /locus_tag="JN755_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7379080.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50791..51570)
                     /gene="hofM"
                     /locus_tag="JN755_000155"
     CDS             complement(50791..51570)
                     /gene="hofM"
                     /locus_tag="JN755_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7379081.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51690..54242
                     /gene="mrcA"
                     /locus_tag="JN755_000156"
     CDS             51690..54242
                     /gene="mrcA"
                     /locus_tag="JN755_000156"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7379082.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54407..54967)
                     /gene="nudE"
                     /locus_tag="JN755_000157"
     CDS             complement(54407..54967)
                     /gene="nudE"
                     /locus_tag="JN755_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7379083.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55287..57422
                     /gene="igaA"
                     /locus_tag="JN755_000158"
     CDS             55287..57422
                     /gene="igaA"
                     /locus_tag="JN755_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7379084.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57487..58155
                     /gene="yrfG"
                     /locus_tag="JN755_000159"
     CDS             57487..58155
                     /gene="yrfG"
                     /locus_tag="JN755_000159"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7379085.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58166..58567
                     /gene="hslR"
                     /locus_tag="JN755_000160"
     CDS             58166..58567
                     /gene="hslR"
                     /locus_tag="JN755_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7379086.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58592..59470
                     /gene="hslO"
                     /locus_tag="JN755_000161"
     CDS             58592..59470
                     /gene="hslO"
                     /locus_tag="JN755_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7379087.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59605..61329)
                     /locus_tag="JN755_000162"
     CDS             complement(59605..61329)
                     /locus_tag="JN755_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7379088.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61708..63330
                     /gene="pckA"
                     /locus_tag="JN755_000163"
     CDS             61708..63330
                     /gene="pckA"
                     /locus_tag="JN755_000163"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7379089.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63406..64758)
                     /gene="envZ"
                     /locus_tag="JN755_000164"
     CDS             complement(63406..64758)
                     /gene="envZ"
                     /locus_tag="JN755_000164"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7379090.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64755..65474)
                     /gene="ompR"
                     /locus_tag="JN755_000165"
     CDS             complement(64755..65474)
                     /gene="ompR"
                     /locus_tag="JN755_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7379091.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65666..66178
                     /gene="greB"
                     /locus_tag="JN755_000166"
     CDS             65666..66178
                     /gene="greB"
                     /locus_tag="JN755_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7379092.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66275..68596
                     /locus_tag="JN755_000167"
     CDS             66275..68596
                     /locus_tag="JN755_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7379093.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69034..69261
                     /gene="feoA"
                     /locus_tag="JN755_000168"
     CDS             69034..69261
                     /gene="feoA"
                     /locus_tag="JN755_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7379094.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69278..71599
                     /gene="feoB"
                     /locus_tag="JN755_000169"
     CDS             69278..71599
                     /gene="feoB"
                     /locus_tag="JN755_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7379095.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71599..71835
                     /gene="feoC"
                     /locus_tag="JN755_000170"
     CDS             71599..71835
                     /gene="feoC"
                     /locus_tag="JN755_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7379096.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72038..72916
                     /gene="rpnA"
                     /locus_tag="JN755_000171"
     CDS             72038..72916
                     /gene="rpnA"
                     /locus_tag="JN755_000171"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7379097.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            72989..73258
                     /locus_tag="JN755_000172"
     CDS             72989..73258
                     /locus_tag="JN755_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7379098.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73252..73617
                     /locus_tag="JN755_000173"
     CDS             73252..73617
                     /locus_tag="JN755_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7379099.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73618..74406)
                     /gene="bioH"
                     /locus_tag="JN755_000174"
     CDS             complement(73618..74406)
                     /gene="bioH"
                     /locus_tag="JN755_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7379100.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74444..75127
                     /gene="gntX"
                     /locus_tag="JN755_000175"
     CDS             74444..75127
                     /gene="gntX"
                     /locus_tag="JN755_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7379101.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75186..75761
                     /gene="nfuA"
                     /locus_tag="JN755_000176"
     CDS             75186..75761
                     /gene="nfuA"
                     /locus_tag="JN755_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7379102.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76122..77426
                     /gene="gntT"
                     /locus_tag="JN755_000177"
     CDS             76122..77426
                     /gene="gntT"
                     /locus_tag="JN755_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7379103.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77471..79555)
                     /gene="malQ"
                     /locus_tag="JN755_000178"
     CDS             complement(77471..79555)
                     /gene="malQ"
                     /locus_tag="JN755_000178"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7379104.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79565..81958)
                     /gene="malP"
                     /locus_tag="JN755_000179"
     CDS             complement(79565..81958)
                     /gene="malP"
                     /locus_tag="JN755_000179"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7379105.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19755 a        22364 c        20778 g        19461 t
ORIGIN      
        1 gtcggggtgt catattcaac gtgagaagtg ttgatggtga taccacgagc tttttcttcc
       61 ggcgcgttat cgatctggtc gaatgcacga gcagcaccgc cgtaggtttt agccagtacg
      121 gtggtgattg cagcggtcag agtagtttta ccgtggtcaa cgtggccgat agtaccaacg
      181 ttaacgtgcg gttttgtacg ttcaaatttt tctttagaca cggctatatt ccttactata
      241 gtgctctccc cttcaggaga gagcacggga ctttggtatt aacccttagg cttatttacc
      301 acgggcttca attacggcct gagcaacgtt actcggcgct tcatcatact tcaggaattc
      361 catagtgtat gatgcacgac ctttggtcag agaacgcagc tgagttgcgt atccgaacat
      421 ttcagacagc ggtacttcag cgtggatctt aacgccagta acttcagatt cctgaccttt
      481 gagcatacca cgacgacggc tcaagtcacc gataacgtca ccggtgttct cttccggagt
      541 ttctacttca accttcatga tcggctcaag cagaactggt ttcgctttct taaagccttc
      601 tttaaaggcg atagaagcag ccagtttaaa cgccagttca gaggagtcaa cgtcatggta
      661 agaaccgaag tgcagacgaa tacccatgtc tactaccggg tagcctgcca gcggacctgc
      721 tttcagctgt tcctggatac ctttatcaac ggccgggatg tattcgccag ggattacacc
      781 acctttaatg tcgttgatga actcgtagcc tttcgggttt gaacccggct ccagcgggta
      841 catgtcgata acaacatgac catactgacc acgaccacca gactgtttcg cgtgtttacc
      901 ttcaacatcg gtaactttct ggcggatagt ttcacggtaa gcaacctgcg gtttacctac
      961 gttcgcttca acgttgaatt cacgcttcat acggtcaacg atgatgtcga ggtgcagttc
     1021 gcccataccc gcgatgatgg tctggttaga ttcttcgtca gtccatacac ggaaagacgg
     1081 gtcttcttta gccagacggc ccagagccag acccattttt tcctggtcag ctttggtttt
     1141 cggttcaact gcgatggaga ttaccggctc agggaattcc atacgttcca gaatgatcgg
     1201 cgcatccggg tcacacaggg tgtcaccagt ggttacgtct ttcagaccga tagcagcagc
     1261 gatgtcgccc gcgcgaactt ctttgatctc ttcacgtttg ttagcgtgca tctgaacgat
     1321 acgaccgaaa cgctcacgtg cagctttcac ggagttcagt acggtatcac cagagttaac
     1381 cacaccggag taaacacgga agaaggtcag gttaccaaca aacgggtcgg tagcgatttt
     1441 gaacgccagt gcagagaacg gctcgtcatc acttgcgtga cgttcagccg gagtgtcttt
     1501 accgtcgtcc aggataccgt tgatcgcagg tacgtcaacc ggggatggca ggtaatcaat
     1561 taccgcatcc agcatcgcct gaacaccttt gttcttgaac gcagaaccac aggttaccag
     1621 gatgatttcg ttgttcagaa cgcgctgacg cagagcacct ttgatttctg cttcagtcag
     1681 ttcttcacca cccaggtatt tttccatcag ctcttcagaa gcttcagctg cggattcgat
     1741 caggttctgg tgccattcgt tagccagttc aaccatgtct gccgggatat cttcgtattc
     1801 gaaggttacg ccctggtcag cgtcgttcca gttgatagct ttcattttca ccaggtcaac
     1861 aacaccggtg aaatgttctt cagcaccaat cgccagctgc agcggaaccg ggttcgcgcc
     1921 cagacgggtt ttgatctggt taacaacttt caggaagttc gcacccatgc ggtccatttt
     1981 gttaacgaac gcaatgcgcg gaactttata tttgtttgcc tgacgccata cggtttcaga
     2041 ctgcggctga acaccaccaa ctgcgcagta aaccattacc gcaccatcga gaacacgcat
     2101 ggaacgttct acttcgattg tgaagtcaac gtgccccggg gtgtcgatga tgttgatgcg
     2161 atgcggctca tactgcttag ccataccaga ccagaatgca gtagtcgcag cggaagtgat
     2221 ggtaatacca cgttcctgct cctgctccat ccagtccatg gttgcagcgc cgtcatgaac
     2281 ttcaccgatt ttatggttta caccggtgta gaacagaata cgttcggtag tagtggtttt
     2341 accggcgtcg atgtgcgcac tgataccgat gttacggtag cgtgcgatgg gtgttgtacg
     2401 agccatttgt ttcctcgttt atcttttagg cgttcaattt aagtagccca aagcgggctg
     2461 cttactggaa gcgcccgcct ggtgactaaa actccgaagg gattaccaac ggtagtgtgc
     2521 gaacgccttg ttggcttcgg ccatacggtg aacgtcttca cgtttcttaa ctgcagtacc
     2581 tttgttttct gcagcatcag aaagttcgtt cgccaggcgc agagccatgg atttatcacc
     2641 gcgtttacga gcagcttcaa cgatccaacg cattgccaga gcattacgac gaaccggacg
     2701 gacttcaact ggtacctgat aagtagaacc accaacgcgg cgagacttaa cttctacagt
     2761 cgggcgcacg ttttcgagag ctacttcgaa tgcttccagt tcagatttac cagagcgctg
     2821 agccagggtc tccagcgcgc tgtatacgat agattcagca gtagattttt taccatctac
     2881 catcaggata tttacaaatt tagccagcag ttctgatccg aacttcggat ccggcagaat
     2941 tttacgctga ccaatgacgc gacgacgtgg catggaaata ctccgttgtt aattcaggat
     3001 tgtccaaaac tctacgagtt tagtttgaca tttaagttaa aacgtttggc cttacttaac
     3061 ggagaaccat taagccttag gacgcttcac gccatacttg gaacgagcct gcttacggtc
     3121 tttaacgccg gagcagtcaa gcgcaccacg tacggtgtgg taacgaacac ccgggaggtc
     3181 tttaacacga ccgccacgga tcaggatcac ggagtgctcc tgcaggttgt gaccttcacc
     3241 accgatgtag gaagtcactt cgaaaccgtt agtcagacga acacggcata ctttacgcag
     3301 cgcggagttc ggttttttag gagtggtagt atatacacga gtacatacgc cacgtttttg
     3361 cgggcatgct tccagcgcag gcacgttgct tttcgcaact ttgcgagcac gtggtttgcg
     3421 taccagctgg ttaactgttg ccattaaata gctcctggtt ttagcttttg cttcgtaaac
     3481 acgtaataaa acgtcctcac acaatatgag gacgccgaat tttagggcga tgccgaaaag
     3541 gtgtcaagaa atatacaacg atcccgccat caccaggcca tctggctgga gtgcttaacc
     3601 gtaagtctga cgaaatcagt atagtcaatg ggaatgatgt cgttcaaaat ttgaacagtc
     3661 aaaccgcggg caataaggtc ttcgttcagg gcatagacct taatgggggc attacgcaga
     3721 ctttcaaggt agcggttacc gtcaactgcg gcagttacgc catcttgcaa taatagcagt
     3781 tcgtctcctt cactgagcag acgcagcagc gcagcaaaat ccgtcagcca gggtgaacga
     3841 tgtaacgtgt gcagcataaa cgcctcaaaa cctcaaaata acatcgtagt tggcgagttc
     3901 gcggcgtaag gcatctgctt cgagcggcgt ggcttcgaca acaaagggtg tctgcggatc
     3961 taacccgcgt tcgcgcagtg aagccgcaca aacccagcac tgttcaatgt cgtacagact
     4021 caacaattta aaagtggcaa tgtaatcacg cgccagcact gcatcgggct tttgtcctga
     4081 cagcagctga aaaacgccat cagcaataaa gaagacggcc agatcgtcag ttaatgcgga
     4141 agttgccagt aaagcatcta aaccttcccg accagcagct gtaccatgag gtgcagtaga
     4201 aaaaacaaac gcaattcgtt tcatcagaac tgtaccaccc tgtcacaggt cagcgaggct
     4261 tccgccagcg cgccaagtcc gcttaaggta aatcccggct gaaggtttga cgaagccagc
     4321 cccagccttc ctgcttcagt ttcatcgacg ataccgcggc gtaacgctgc cgctacgcag
     4381 atattcagcg ccacaccatg ttgcgcattc aactgttgcc agccgcgcac aaggtcaaat
     4441 tcgtcgcttg ccggtgaggt cagttggtta gcgttataga ccccttcccg atagaaaaag
     4501 acgctgctta acttatggcc ttctgctatc agcgcctgcg caaactgaaa agcactactc
     4561 gcctgttgcg taccgtatgc tggcccggtc accacgatgg caaaacgcat tacttatctt
     4621 gcccctgaaa atcaccgctc ttgaactggc ggatgtagag atagacagtg tgtttggaga
     4681 tgttcaggcg gtcagcaacc tggttgatcg catctttaat atcgaagatc cctttctcgt
     4741 agagattcag cacgatctga cgatttttgg cgttattaga aacattgcga tcggcattca
     4801 cttcttcgat ggtgaactcc agcgtttggg taaccagatc ttcaacagaa gaggcaaagt
     4861 tgacgcttga accgacatcc ggggtttctg gcggcacaaa agtgctcata atctgcgaga
     4921 agggaacatc aagattcata ttgatgcaca gcagaccaat tacacgctgt tcgcggttac
     4981 gaatcgcgat agtcagggac ttcattaata cgccgctttt ggcgcgagta aagtagcatt
     5041 tagaaacgct gctatccgct cccgtcatat cgtgcagcat acgtagcgcc aggtcagtaa
     5101 ttggcgaacc aatcttccgg cctgtatgtt caccgttagc aatgcgaatg gctgaacatt
     5161 ttagatcctg caaagagtgc aaaacgattt cacagtggga gccaataagc atcgctaacc
     5221 cgtccaccac cgcttcgtag gatttcagaa tatcaaaatc ggtctggtcg aaaggacgtt
     5281 gatccagtaa atccaactca ctggtttcgt tggttaaaag cgacctggac atgaaaaaaa
     5341 acactccttt tcaggagcct gtcgttaact tttcagggca ggctcattaa tgatgcgggt
     5401 aactaaatta atacagcgga ggttccgctt tccagcacta attatatccg gcctgtaata
     5461 aaaaaaccgc cgcctggtca ggcggcggtt cttaatgctt attttttagc agaatctgcg
     5521 gctttcgcat cagcttccgg ctttgcatca gccttcggcg ctggtttcac atccagcagc
     5581 tctacgtcaa acactagggt agaattcggt gggatccccg gaacacccgc tttgccgtaa
     5641 gccagttctg gtggaataac cagtttgatc ttaccgcctt tcttgatgtt cttcagacct
     5701 tctgtccaac ccgggataac accgtccaga cggaaagaaa gcggttcacc acgggtgtaa
     5761 gagttgtcga actctttacc gtcgatcagc gtacctttgt agttcactac aacagtatcg
     5821 ctgtctttcg gtgcttcgcc tttaccggct tctactacct gataaaccag accagttgaa
     5881 gaggttttca cacctttctc tttggcaaat ttctcgcggt actctttacc ttttgcttcg
     5941 ttatcagccg cgtctttttc catcttcgcc tgagcagaag acttcacgcg agcttcgaat
     6001 gcttgcagag tctgttcgat ctcttggtcg gagagtttgc tcttatcagc aaatgcatcc
     6061 tgaacaccag cgatcagctg atctttatcc agtttgatgc ccagtttttc ttgttcttta
     6121 agagagtttt ccatgtaacg acccagcgaa gcacccagtg cataagctga tttctgatcg
     6181 tcatttttga acgctgcttt gctgtcagca gttgtagcag gttttgcagc ttcagcagca
     6241 aaagtgattg gtgcatgcag ggcaacggcc attgtggtcg ccagtagcgt tactttaaac
     6301 agtgatttca tccatatctc caggatcggg gcatctcacc ccagggttaa ctattatcag
     6361 aagggtacta taaagcgttg tcgaacaaat ctacatacag acacgcccta ttatcatcta
     6421 ttttcagact ctttttgttt aaattagttt cgatgaccgc gaaatgagtg ctgtctcggg
     6481 cagcaaagtt aagtagaatc cgcggcgacc attcgacaaa agaggtgaat catgcaggat
     6541 ttatcattgg aagcacgcct ggcagagctg gagagccgac tggcttttca ggaaatcacc
     6601 attgaagaac tgaacgtcac ggtgaccgct catgaaatgg agatggcgaa actgcgcgat
     6661 catctgcgtc tgctgaccga gaagttaaaa gccagccagc cgtcgaacat cgcgtcgcag
     6721 gctgaagaaa cgccaccgcc acattattga ggcgtaaaaa aagcggggat tccccgcttt
     6781 tttgtcacct tttcggtatt agtggcaacc acaaccgccg ttgcctttac cgccacagca
     6841 gccttcgcca ccgtgttcat gaccgtgatc gtggccatga ccgccgcagc aaccgtcgtg
     6901 gtcgtgatcg tggtgatgat cgtgcgcgcc gtgaacgtgg ccatgagcca gttcttcttc
     6961 ggtcgcttcg cgaatcgcca caacttcaac gttgaatttc aggttctgac cggccagcat
     7021 gtggttacca tcaaccacta cgtgatcgtc ttcaaccgca gtgatttcaa ccggtaccgg
     7081 accctgctca gtttcagcca ggaaacgcat acctacctgc agttcatcaa cgcccataaa
     7141 tacgtcttta ggaacacgtt gcactaggtt ttcgtcgtac tgaccgtaag cgtcgttcgc
     7201 gccaacagcg acatcaaatt tgtcgccaac ttcatgacct tccagcgccg tttccaggcc
     7261 agagatcagg gaaccgtgac catgcaggta gtccagcggc gcactcaccg gagactcatc
     7321 aaccaacaca ccgtcttctg tacgtacctg ataggccagg ctgaccacca ggtcttttgc
     7381 tactttcatg atatctcctg agcatgggaa gaatagtggc gcagattgta gcggaattct
     7441 gcagccgtgt actcactagc ttaaaaaaac tcggcgcata tcgctagtcc ggatgaaaaa
     7501 tcccgatcac ttgctcatct ttgtgaacgt gatcgcgggc ttctttgtct gcttctcgca
     7561 tctgatgtcc gcacttaaca cattcaacaa tatcaatatt attttcgcgc cacatcgcca
     7621 ttgaatcctg cgcctgacag gccgggcatt ttgcgcccgc tataaaacgt tttcggattg
     7681 ccatccttac cctctactca aattcatccc agccgtccag ctggcgtcgt tcttgttgca
     7741 tttcacgctg gaaaatctct tccagttcgc gtcgggcttc cctggcgcga gaaatttgta
     7801 cggtatcagc atgcatcggg attaactccc gcagcattcg catatccagg cggcgaaaat
     7861 gcagttgcgc ccgctgtgcc tgatgcggat gcatgccaag cgtgaccagc gtcttgcgcc
     7921 ccagctctaa cgcactggag aatgtttcac gggaaaactg cgtcacccct gcctgtaata
     7981 actcatgcgc ttccacacgt ccgcgcactc gcgcaagaat atgcaaatgc ggaaagtgct
     8041 gctggcatat ttccaccagc ttcatggtgt cttccggctc gttacaggta atgacgatag
     8101 actcagcggc ctctgcaccc gcagaacgta aaagatcgac ctgcgtggcg tcgccgtaat
     8161 aaactttgta gccatattta cgcatcaggt taacggcgct gatatcccgc tccagcacgg
     8221 taatgcgcat tttatttgcc atcagcaaac gaccaatcac ctgaccaaaa cgcccgaagc
     8281 ccacgacaat gacctggggt ttatcatcgt tgacccacgg tttttcgtct tcttcttccg
     8341 gtccgttaaa ctggcgggat agccatttat ccaccagctt catcagcaac ggcgtggtca
     8401 tcatggaaag cgtcaccgtc accagcaaca gcgccatctg gtcgccctgg aataaacgtt
     8461 gtgaagaagc ggtagaaaag aggacaaagg caaactcacc gccctgactc aacacgccag
     8521 caaactgcat ccgctctgag ctacgcacgc catacaatcg cgccagcaga tacagcacga
     8581 gaattttcac cgccaccagc acaaccacgc ttatcactac ccacaacaga tgggtataaa
     8641 gcaccccgag gttgagcgac atgccgacag agataaagaa caaaccgagc agcaagcctt
     8701 tgaagggatc gatagccgtt tccagttcat ggcgatattc actttccgcc agcagcacgc
     8761 ccgcaataaa tgtaccgagc gccatcgaca gccccagcgc atccataaac aatgcggaac
     8821 ccaacaccag cagcagcgtc gcggcggtga acacttcccg aacgccagaa gctgcaataa
     8881 agcggaatac cggacgcagt aaatagcgcc caccaatcag catgccgaca aacgccagca
     8941 ccttcatgcc gatcttcatc cagtcgaaat gttcgtctgc cgaccccgcc aacaacggca
     9001 ctaacgccag tgctgggatc actgccagat cctgaaacag cagaaccgaa aacccgagct
     9061 ggccggattc gctgcgattc atccctttct cacgcatcaa ttgcaacgcc attgcagttg
     9121 aagacatcgc aaggccaatg ccaccgacca ccgccgcctg ccaggcgaaa tccgtcagca
     9181 tcaataatcc cgccagcaac gccgcgctta acagcacctg tgccacgcct acgccaaaaa
     9241 tcgaacgccg cagttgccaa agtttggagg gattcaactc aaggccgatg ataaacatca
     9301 ggaataccac gccgagttcc gaaaagtgga ggatctcatc gacgtcgcta ataaacccca
     9361 gcccccacgg gccaattgca atccctgcca gcaaatatcc caacacagcg ccaatcccca
     9421 gccgcgatgc caacggcacc gcagccaccg ccgcgaagag aaacagcact cctgcgagta
     9481 aaaaatcgga accttccatc agcggcctcc tggagacagc ggatttgcca accagtcacc
     9541 gtaggctctg gcgtggctcg ccagctcctt tgcgctttgc cgtctcgccc agtaaataat
     9601 gatggaactt aaccaatgca tccggcacat gcccgccgcc agttcaaagg ggcgcagcac
     9661 atcgctcatc gggtagcgat tcagcgcgtc ataacggtaa gcactttccg gctcgccggt
     9721 ggtaatcacg ttacgccagt actttcccgc cagttggttt cctcccggcc cgctggcaaa
     9781 accacgactt aatacccggt ccagccactc tttcagtagc gccgggcagc tataggtata
     9841 aagaggatgc tgaaagacaa tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat
     9901 atcaataaaa aaatcgggat agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg
     9961 cgtggccggt ttaagcagta cccggtttgc caccgagtcc tgagattccg gatgggcata
    10021 cagcagcaaa actttcgctg gctgagacat catccccctc ccggtatggt ttttgtgtat
    10081 agtcgctgtt ttgggctacc attgcgcccg gtgcggcagc tcgcccatac attacattat
    10141 cataatgata agttaacata gtctgaacat acggcgcctt atgattgttt tctcctcgtt
    10201 acaaattcgt cgcggcgtgc gcgtcctgct ggataatgcc accgccacca tcaaccccgg
    10261 gcagaaagtc ggcctggtgg gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct
    10321 gaaaaatgaa atcagcgccg acggcggcag ctacaccttt ccgggaagct ggcaactggc
    10381 gtgggtgaat caggaaacgc cagcgttacc gcaagcggcg ctggaatatg tcattgacgg
    10441 cgaccgtgaa tatcgtcaac tggaagcgca gctgcacgac gccaacgaac gtaacgacgg
    10501 gcacgccatt gcgaccattc atagcaagct ggatgctatt gacgcatgga gtattcgctc
    10561 ccgtgccgcc agcctgctgc acggcctcgg tttcagcaat gaacaactgg agcgcccggt
    10621 aagtgatttc tccggtggct ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg
    10681 ttcagacttg ctgctgctcg acgaaccgac taaccacctc gatctcgatg ccgttatctg
    10741 gctggaaaaa tggttgaaga gctatcaggg cacgctgatc ctgatctctc acgaccgcga
    10801 cttcctcgat ccgatcgttg ataaaattat tcagatcgaa caacaaagca tgttcgagta
    10861 caccggcaac tacagttcgt ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc
    10921 gatgtacgaa agccagcagg aacgcgtggc acatctgcaa agttatatcg atcgtttccg
    10981 tgccaaagcc accaaagcga agcaggccca gagccgcatt aagatgctgg aacgtatgga
    11041 gctgattgcc cccgcacacg tcgacaaccc gttccgcttt agcttccgcg cgccggaaag
    11101 cctgccaaat ccgttactga agatggaaaa ggtcagcgca ggctatggcg atcgcattat
    11161 tctcgactcg attaaattga atctggtccc cggctcgcgc attggtctgt taggccgcaa
    11221 cggcgcaggt aaatcgacat taatcaaact gttagccggt gaacttgcgc cagtcagcgg
    11281 tgaaattggt ctggcgaaag ggatcaagct cggctacttc gcccagcatc aacttgaata
    11341 cctgcgcgcc gacgaatcac ctattcaaca tctggcacgt ttagcgccgc aggagctgga
    11401 acaaaaactg cgtgactacc tcggcggctt tggtttcctg ggcgataaag taaccgaaga
    11461 aacgcgccgc ttctccggtg gggaaaaggc ccgcctggtg ctggcattaa ttgtctggca
    11521 gcgtccgaat ctgctgctgc tcgacgaacc gactaaccac cttgacctcg acatgcgtca
    11581 ggcagtcacc gaagcattaa tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg
    11641 tcatttgctg cgttccacca ctgacgatct ctacctggtt cacgatcgta aagtcgaacc
    11701 gttcgacggc gatctggaag attatcaaca gtggttgagc gacgtacaaa agcaggaaaa
    11761 ccagaccgac gaagcgccaa aagagaacgc gaacagcgcc caggcacgta aagatcagaa
    11821 gcgtcgggaa gcggagctgc gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct
    11881 ggaaaaagag atggagaagc tgaacgcgca actggcgcag gcggaagaga aactcggcga
    11941 cagcgaactg tatgaccaga gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc
    12001 cagcgccaaa tccggcctgg aagagtgcga aatggcgtgg ctggaagccc aggagcagct
    12061 tgagcagatg ctgctggaag gccaaagcaa ctgatggcgc agataacgac gaccgatgcc
    12121 aatgaattta gcagcagtgc tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta
    12181 caaactatgc tgccgcgttt gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg
    12241 ctggagttgc ccgacggcga ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg
    12301 aaacataaac cgcgtttagt ggtgtttcac gggctggaag gcagtctcaa tagcccttac
    12361 gcccacggtc tggttgaggc ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt
    12421 cgcggatgca gcggtgaacc aaaccgtatg caccgcattt accattcggg cgaaaccgaa
    12481 gacgccagtt ggtttttacg ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc
    12541 gtcggctatt cgctcggcgg taatatgctg gcctgtttgc tggcaaagga aggcaatgat
    12601 ctcccggttg atgcggcggt gattgtctct gcgccgttta tgctggaagc ctgtagctat
    12661 catatggaaa agggcttttc ccgcgtttat cagcgttact tgctgaacct gttaaaagcc
    12721 aatgccgcgc gcaagctggc agcctacccc ggaacgctgc cgattaatct cgcgcagtta
    12781 aaatcggtac gtcgcatccg tgaatttgac gatctaatca ccgccagaat tcacggctac
    12841 gccgacgcta tcgactatta tcgtcagtgt agcgccatgc cgatgctgaa ccggatcgcc
    12901 aaaccgacgc tgattattca cgccaaagac gatccgttta tggatcatca ggtgatccca
    12961 aaaccggaaa gtctcccctc gcaggtggag tatcaactga ctgaacatgg cggtcatgtt
    13021 ggctttattg gcggtacatt acttcatccg caaatgtggc tggagtcacg cattcctgac
    13081 tggttaacaa cgtatctgga ggcgaaatca tgttgattcc gtggcaagac ctctcccccg
    13141 aaacgctgga aaatttaatt gaaagctttg tgttacgtga aggcaccgat tatggtgaac
    13201 atgagcgtac acttgaacag aaagtcgccg acgtcaaacg ccagctacag tgcggagaag
    13261 cggtgctggt atggtcggaa ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc
    13321 gcgaataacc tgcaaccgtc ggcggacgag ataatagtga tagctaagtc atggagttac
    13381 tatgtctgcc aaacatccgg tcattgcggt aacaggatcc agcggcgcgg ggaccaccac
    13441 caccagcctc gcgtttcgta aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga
    13501 aggcgacagt tttcaccgtt acacccgccc ggaaatggac atggcgatcc gcaaagcgcg
    13561 cgacgccggg cggcatatca gctacttcgg ccccgaagcg aacgacttcg gcctgctgga
    13621 acaaaccttc attgaatacg gtcagagcgg caaagggaaa tctcgcaaat atctgcatac
    13681 ctacgacgaa gccgtaccct ggaatcaggt accgggaaca ttcaccccct ggcaaccttt
    13741 accagaaccc actgatgtac tgttttatga aggtttacac ggcggcgtag tcacgccaca
    13801 gcataacgtt gcgcagcatg tggacttact ggtcggcgta gtgcctatcg ttaaccttga
    13861 gtggattcaa aaactgatcc gcgacaccag cgagcgcggg cactcacgag aagcagtgat
    13921 ggactcggta gtgcgttcaa tggaagacta tatcaactac atcacaccgc agttttcccg
    13981 cacccatctt aacttccagc gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa
    14041 aggtatcccg tcgctggatg aaagctttgt ggtgatccat tttcgtaatc tggaagggat
    14101 cgatttccct tggctgctgg cgatgttgca aggctcattc atttcccaca tcaatacgtt
    14161 agtggtacct ggcggcaaaa tggggctggc aatggaatta attatgctgc tgctggtgca
    14221 acgattgatg gaaggaaaga aaatcgagta actttgctat tatgccggat aaaatactat
    14281 ccggcttcac aacgggatag ttaagtcacg cggcaaccac ttcatacgag tgagtaatat
    14341 tcaccgcttt ttccagcatc aacgccactg aacaatattt ctcggcagag agatcaaccg
    14401 cacgcgcaac cgctgcgtct ttcaggtcgc gaccggtgac gataaaatgc agattaatgt
    14461 gcgtaaacag gcgtggtgcc tcttcgcggc gttcagaggt caattttact tcacaatcga
    14521 ccacatcctg acgccctttt tgcaggatcg aaaccacatc gatggcactg caaccacccg
    14581 ccgccatcaa caccatttcc atcggacttg gtgctttatc gcctgagttg ccgtccatta
    14641 aaatctggtg accagaggcg gattcgccca gaaaagttaa cccttcgacc cacttcactc
    14701 gcgcttgcat ttttgctact ccactgcgtc aattttcctg acagagtacg cgtactaacc
    14761 aaatcgcgca acggaaggcg acctgggtca tgctgaagcg agacaccagg agacacaaag
    14821 cgaaagctat gctaaaacag tcaggatgct acagtaatac attgatgtac tgcatgtatg
    14881 caaaggacgt cacattaccg tgcagtacag ttgatagccc cttcccaggt agcgggaagc
    14941 atatttcggc aatccagaga cagcggcgtt atctggctct ggagaaagct tataacagag
    15001 gataaccgcg catggtgctt ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt
    15061 ctcattgcca cattcataag tacccatcca agagcacgct tattcaccag ggtgaaaaag
    15121 cggaaacgct gtactacatc gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg
    15181 gtaaagaaat gatcctctcc tatctgaatc agggtgattt tattggcgaa ctgggcctgt
    15241 ttgaagaggg ccaggaacgt agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg
    15301 aaatttcgta caaaaaattt cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc
    15361 tgtctgcaca gatggcgcgt cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt
    15421 tcctcgacgt gacgggccgc attgcacaga ctctgctgaa cctggcaaaa caaccagatg
    15481 ctatgactca cccggacggt atgcaaatca aaattacccg tcaggaaatc ggtcagattg
    15541 tcggctgttc tcgtgaaacc gtgggacgca ttctgaagat gctggaagat cagaacctga
    15601 tctccgcaca cggtaaaacc atcgtcgttt acggcactcg ttaatcccgt cggagtggcg
    15661 cgttacctgg tagcgcgcca ttttgtttcc cccgatgtgg cgcagactga tttatcaccc
    15721 cgatatcaac tatgcacttc gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt
    15781 aatgcttggc gaattacgat tcggtctgct cttctccctc gttcctgcct gttgcaatat
    15841 tgcgggcctt gatacgcctc ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct
    15901 gtttgccacc tgtagcttac tgacacagct gctactggta aaagatgttc ccctgccctt
    15961 tttgctgacc ggattaacgc tggtacttgg cgtcactgct gagctggggc cattgcacgc
    16021 aaaattgctt cctgcatcgc tgctcgccgc catttttacc ctcagtctgg cgggatacat
    16081 gccggtctgg gaaccattgc tcatctatgc gttgggcact ctctggtacg gattgtttaa
    16141 ctggttttgg ttctggatct ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta
    16201 ccgtgaactg gcagattatt gtgaagccaa atacagcctg cttacccagc acaccgatcc
    16261 tgaaaaagcg ctgccaccgc tgctggtgcg ccagcaaaaa gcggtcgatt taattactca
    16321 gtgctatcag caaatgcata tgctttccgc gcaaaacaat accgattaca agcggatgct
    16381 gcgtattttc caggaggcgc tggatttgca ggaacatatt tcggtcagtt tgcatcagcc
    16441 ggaagaggtg caaaaactgg tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc
    16501 gcaaaccgtc gccgctcgcc tgcgcgtgct ggctgatgac attctttacc atcgcctgcc
    16561 aacgcgtttt acgatggaaa agcaaattgg cgcactggaa aaaatcgccc gccagcatcc
    16621 ggataatccg gttgggcaat tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg
    16681 cacccaaaaa ccgctctatg cccgtgactt actggccgat aaacagcggc gaatgccgtt
    16741 gcttccggca ctgaaaagtt atctgtcact aaagtctccg gcgctacgca atgccggacg
    16801 actcagtgtg atgttaagcg ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc
    16861 gtactggatc ctgatgacgg tattgctggt gacacaaaat ggctatggcg caacccgtct
    16921 gaggattgtg aatcgctccg tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct
    16981 gcactttaaa attcccgaag gttacaccct gacgttgatg ctgattacca ccctcgccag
    17041 ctacctgata ttgcgcaaaa actacggctg ggcaacggtc ggttttacta ttaccgcagt
    17101 gtataccctg caactgttgt ggctgaacgg tgagcagtac atccttccac gtcttatcga
    17161 taccattatt ggttgtttga ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca
    17221 gagcgggtta ttgcgtaaaa acgcccatga tgctttagaa gcctatcagg aagcgattcg
    17281 cttgattctg agcgaggacc cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa
    17341 tcaggcgcat aacactctgt ataactcatt gaatcaggcg atgcaggaac cggcattcaa
    17401 cagccattat ctggcagata tgaaactgtg ggtaacgcac agccagttta ttgttgagca
    17461 tattaatgcc atgaccacgc tggcgcggga acaccgggca ttgccacctg aactggcaca
    17521 agagtattta cagtcttgtg aaatcgccat tcagcgttgt cagcagcgac tggagtatga
    17581 cgaaccgggt agttctggcg atgccaatat catggatgcg ccggagatgc agccgcacga
    17641 aggcgcggca ggtacgctgg agcagcattt acagcgggtt attggccatc tgaacaccat
    17701 gcacaccatt tcgtcgatgg catggcgtca gcgaccgcat cacgggattt ggctgagtcg
    17761 caagttgcgg gattcgaagg cgtaatacag gctaaaccat cggataaggc attcacgccg
    17821 catccgacat attttgcctg atgcgtctta tttacgcccc aaccaccttc gccaccgcgt
    17881 gggcgaaacg ttgcatcccc tcatcgatat ccgcatcttc caccaccagc gacggtgcta
    17941 aacgcatcac atccggtccg gcattcagca ccattacgcc aacctctgca cccgcataca
    18001 ggaaatcacg cgcccgacct ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca
    18061 taccgcgaat atcgctaaac acatcgtact gctgatcgat cttctgcaaa tggtcaacaa
    18121 aacgctggcg tttcgcctga atgccttcca gcacttcagg ggtattgatg atatcaaacg
    18181 ccgcgcccgc tactgcacag gccagaggat taccgccgta ggtggaaccg tgagaaccag
    18241 gatgaaacgc agaagcaatt tccgccgtgg tcagcatggc gctaatcggg aagccgccgc
    18301 ctaacgcttt cgcagaagtc agaatatccg gcgtcacacc gtagtacctg taagcaaaca
    18361 aatcgccagt ccgccccatc ccgcactgca cttcatcaaa caccaataat acctgatgtt
    18421 gatcgcacag ctcgcgcaag ccctgcaaaa actctggcgt cgctgccgtc acaccgccct
    18481 cgccctggat cggctcaacc accaccgcac aggtgtgatc atccatcacc gctttcactg
    18541 catggagatc gttaaaggga acgtggatga tgtctgccgg ttgcggccca aagccgtcgg
    18601 aatattttgg ctgcccaccc accgaaacgg taaacagcga gcgaccatga aaagcgttat
    18661 ggaaggcaat aattttggtt ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca
    18721 gtttaaaggc ggtttcgtta gcttccgtgc cggagttcat aaacaccacg cgttcggcaa
    18781 acgtcgcctc aatcagtttg cgcccaagac gcagcgccgg ttcattggtg aaaacgttac
    18841 tgatatgcca cagagtttcg ccctgggttt ttaacgcgtt caccagcgca ggatggcaat
    18901 ggcccaacgc cgtaactgca atgccacccg cgaaatcgac atactccttg ccttgctgat
    18961 cccagattcg gctgccctga ccttttaccg gaataaactc tgccggagca taaatcggca
    19021 ggatcacttc atcgaaagtc gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat
    19081 caccctgtta cgcataaata aatatgaaat tataatcaca aaatatgcat aaaaaatcac
    19141 tgaatggcaa tcggaaatca gagatgcagg aaattagcca gcagttgatg tccttgttcg
    19201 ctaagaatac tttctggatg gaactgcaca ccttccagat cccactggcg atggcgaatc
    19261 cccataatct cgcgggtttc gctccaggcc gtcacgtcaa agcacgctgg taatgagtca
    19321 ggttccacca ccagcgaatg gtagcgtgtc acggtaagtg gatttgccag cccccgaaat
    19381 acgccctcac cgctatgtgt aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc
    19441 acaactttgc cgccaaatgc ctgcgccatt gcctgatgac cgaggcagac gccaagaatc
    19501 ggcaagcgcc cggcatagtg gcgaataacg tcaagggaga tcccggcttc atctggcgta
    19561 caggggccag gtgagatgac aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc
    19621 aacgcatcgt tgcgcttaac cagcacatcc gcccccagtt cacaaaagta ctggtagagg
    19681 ttccaggtaa aagaatcgta gttatctata agcaggatca tggcggctcc ggtacgaaag
    19741 aaccgcacta ttttactcag attccccggc ttcgcttacc actttgctaa atatcatctg
    19801 cagtgcggtg agatccccca ttgccccact ctgatttgcc tgattccagg cctctttttc
    19861 gataccctgc cagctcagtt gatatccggc atgaatcgcc agttgctcga agaagatccg
    19921 ctgtgccaga ccacttccca cccggaaggg atgcagcacg tcgatttcac aatagtaatg
    19981 cgccagccgc tcgacgaact tcgctttctc caggccaacc agataacctt cttcctccag
    20041 atcctgcatc agggcattgc cctctttttc gatataagca aagtggcaga acggcgtatc
    20101 gccctgataa atatcaactt cacgcagttg ccctgcccag tcgaaaatat cctggtacag
    20161 ctggcgatgg atagtgcgca aatgcggtaa accgcgcacc agcggaccaa gctcaatggt
    20221 cgcagcacgc agcgccgtca tttcgtaagc ggcctgttcc agccgctgct gctggcggat
    20281 gttcagccgg ttacgcatga tatcaaggcc aggataaaga tacggatcgc gcccttcgcc
    20341 gaatttatcg ctcatagtgc ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag
    20401 taagagtgac taaaggcatc tcgacgcctt caagacggcg actggcctgg aaattacgat
    20461 tacgctgaag ctcccagaga cgggactttt gcttatcggt gagtttcttc acttgatgcc
    20521 tccctgaacg tgtccatttg ccacaagtat aagcggcaaa tccgggttac gccgggagag
    20581 cagaagcgcg ggcaagattg cccgcgcgag aaatcattac ggcaggactt tagcggaaag
    20641 gataactacc ggttttgacg gcacattctg gtacggacca acatcatgag tcggcacctg
    20701 ggaaatctta tcggcaacgt ccatgccttt caccacttta ccaaataccg cgtaaccgaa
    20761 atcacgctga ccatggtcaa ggaaggcgtt atcggcaacg ttgataaaga actggctggt
    20821 ggcgctgtct ttgtcagcgg tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc
    20881 gttatcggct tcatttttga ttggcgggtt tggttttttc tgctgcatct gctcggtgaa
    20941 accgccgccc tgaatcataa agccaggaat gacgcggtga aaggtagtgt tgttataaaa
    21001 accgctgttc acataatcga caaagttttg cacagacact ggcgcttttt gtttatccag
    21061 ctccagttcg atgttaccag ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc
    21121 cattgctgcg ggagaaagag cagaaagagc gaaaacagca gccatcgccg ccagggtcga
    21181 tttgaacatg agatttcctt aacaaacaga gcagaaaaaa agcaagtgaa gagattctaa
    21241 atagccttca agaacaaggc catcccttta cctaatttta cgtatctgaa atatatgtaa
    21301 cctgacgatc actttttatt ccgtaatcaa aatcacctct taaaatgcaa tttagcaacc
    21361 gattgcaata aaacatttaa acagatcaca aaatcaccta aaatcgcccg ctcgcggcat
    21421 atcccgctat gccattttat ttttgaacgc aggccaatca tgactaacag caatcgcatc
    21481 aagctcacat ggattagctt tctctcctac gcactgactg gtgcgttggt tattgtcacc
    21541 gggatggtga tgggaaatat cgccgattat ttcaatctgc ctgtttccag tatgagtaac
    21601 accttcacct tcctcaacgc cggcatttta atctctatct tcctcaacgc ctggctgatg
    21661 gaaatcgtcc cgttgaaaac gcagttacgt tttggctttc tcctgatggt gctggcggtt
    21721 gccggtttga tgttcagcca cagcctggcg ctgttctcga cggcgatgtt cattctcggg
    21781 gtggtcagcg gcattactat gtcgattggt acattcctga taacacaaat gtatgaaggg
    21841 cgtcagcgcg gttcccgcct gttatttact gactccttct tcagtatggc tgggatgatt
    21901 ttcccaatga tcgccgcgtt tctactggcg cgcagcattg agtggtactg ggtttatgcc
    21961 tgcatcgggc tggtgtatgt cgctattttt attctgacct tcagctgtga gttcccggca
    22021 ctgggcaaac gtgcaccaaa aacggatgct ccggtagaga aagaaaagtg ggggatcggc
    22081 gtactgtttc tctctgttgc ggcgctgtgc tacatcctcg gtcagttagg ttttatctcc
    22141 tgggtgcctg agtatgccaa aggcctgggc atgagcctga acgacgcggg cacgctggtg
    22201 agtaacttct ggatgtcata catggtcggc atgtgggcgt tcagctttat tcttcgcttc
    22261 tttgatttgc aacgcattct gaccgtactg gctggtctgg ctgcgattct gatgtacgtc
    22321 tttaacaccg gaacaccagc acatatggcg tggtcaattc tcgctctggg cttcttctcc
    22381 agcgcgatct ataccaccat catcaccctg ggttcacagc agaccaaagt accgtcgcca
    22441 aaactggtta actttgtcct gacctgcgga accatcggta ctatgttgac ctttgtggtt
    22501 accggcccga ttgttgaaca tagcggtccg caggcggcac tgcttacagc aaacggtctg
    22561 tacgctgtcg tctttgtgat gtgcttcctg ttaggtttcg tcagccgtca ccgtcagcat
    22621 aacaccctga cctctcatta attgctaatg ccggacggca ctatcgtcgt ccggcctttt
    22681 cctctcttcc cccgctacgt gcatctattt ctataaaccc gttcattttg tccatttttt
    22741 gcacaaacat gaaatgtcag acaattccgt gacttaagaa aatttataca aatcagcaat
    22801 atacccctta aggagtatat aaaggtgaat ttgatttaca tcaataagcg gggttgctga
    22861 atcgttaagg taggcggtaa tagaaaagaa atcgaggcaa aaatgagcaa agtcagactc
    22921 gcaattatcg gtaacggtat ggtcggccat cgctttatcg aagatcttct tgataaatct
    22981 gatgcggcca actttgatat taccgttttc tgtgaagaac cgcgcatcgc ttatgaccgc
    23041 gtacacctct cgtcttactt ctctcaccac accgccgaag agctgtcgct ggtgcgcgaa
    23101 ggcttctacg agaaacacgg catcaaagtt ctggtcggcg aacgcgctat caccatcaac
    23161 cgtcaggaga aggtgattca ctccagcgcc ggacgtaccg ttttttatga caagctgatc
    23221 atggcaaccg gttcctaccc gtggatccca ccaatcaaag gttctgatac tcaggactgc
    23281 tttgtctatc gcaccattga agacctcaac gccattgaat cctgcgcccg tcgcagcaaa
    23341 cgcggtgccg ttgttggtgg cggcctgtta ggtctggaag ccgctggcgc gctgaaaaac
    23401 ttaggtattg aaacccacgt tatcgaattt gcccctatgc tgatggcgga acagcttgat
    23461 cagatgggtg gcgagcagct gcgtcgcaaa atcgaaagta tgggcgtgcg cgttcacacc
    23521 agcaaaaaca cccttgagat tgtgcaggaa ggtgtcgaag cgcgtaaaac catgcgtttt
    23581 gccgacggca gcgaactgga agtcgacttt atcgtcttct ctaccggtat ccgtccgcgc
    23641 gataagctgg caacccagtg tggtctggac gttgctccgc gtggggggat tgtcattaat
    23701 gattcctgcc agacttccga tccggatatc tacgccatcg gtgaatgcgc aagctggaac
    23761 aaccgtgtat ttggtctggt agcaccaggc tacaaaatgg cgcaggtcgc cgttgaccat
    23821 attctgggta gcgaaaacgc ctttgaaggt gctgacctta gcgccaagct gaaactgctg
    23881 ggcgtagacg taggtggtat tggtgatgcg cacggtcgca cgcctggcgc acgtagctat
    23941 gtttacctcg acgaaagtaa agagatctac aaacgcctga ttgtcagcga agacaacaaa
    24001 accctgctcg gtgcggtact ggtgggcgat accagcgact acggcaacct gctgcaactg
    24061 gtgctgaacg ctatcgagct gccggaaaac ccggattctc tgattctgcc ggcgcactcg
    24121 ggtagcggta agccgtctat cggtgttgat aaactgccgg acagcgcgca aatctgctcc
    24181 tgcttcgacg tcactaaagg tgatctgatt gctgccatca acaaaggctg ccacacagtt
    24241 gcggcgctga aagctgaaac caaagcgggt actggctgcg gtggctgtat cccgctggtc
    24301 actcaggtac tgaacgcgga actggcgaaa cagggcatcg aagttaacaa caacctgtgc
    24361 gaacactttg cttattcgcg tcaggaactg ttccatttga tccgcgttga aggcattaaa
    24421 accttcgaag aactgctggc gaaacacggc aaaggctacg gttgtgaagt ttgtaaacca
    24481 accgtcggtt cgctgctggc ctcctgctgg aacgaataca ttctgaagcc ggaacatact
    24541 ccgctgcagg attctaacga caacttcctc gctaacatcc agaaagacgg cacctactcg
    24601 gtgatcccgc gttctccggg cggtgaaatc accccggaag ggctgatggc agtgggtcgt
    24661 atcgcgcgta aatttaatct ctacaccaaa atcaccggct cccagcgcct ggcgatgttt
    24721 ggcgcacaga aaggcgatct gccggaaatc tggcgtcagc tgattgaagc tggcttcgaa
    24781 accggtcatg cctatgcgaa agcactgcgt atggcgaaaa cctgcgtggg tagcacctgg
    24841 tgccgctacg gtgttggcga tagcgtcggc ctcggcgtgg aactggaaaa ccgctacaaa
    24901 ggcatccgta cgccgcacaa aatgaagttc ggtgtctccg gctgtacccg tgaatgttca
    24961 gaagctcagg gtaaagacgt gggtattatc gccactgaaa aaggctggaa cctgtatgtt
    25021 tgcggtaacg gcggcatgaa accgcgtcat gcggatctgc tggcagcgga tatcgatcgc
    25081 gaaacgctga tcaaatatct cgaccgcttc atgatgttct acatccgtac cgccgacaaa
    25141 ctgacgcgta ccgcgccgtg gttagaaaac ctcgaaggcg gcatcgatta cctgaaagcg
    25201 gtgatcattg acgacaaact ggggctgaac gctcatctgg aagaagagat ggcgcgcctg
    25261 cgtgaagcgg tagtgtgtga gtggactgaa acggtcaata caccgtccgc gcagactcgc
    25321 ttcaaacact tcatcaacag cgacaagcgt gacccgaacg tgcagatggt gccagagcgc
    25381 gaacagcacc gtccggcaac gccgtatgaa cgtatcccgg taactctggt ggaggacaac
    25441 gcatgagcca gtggaaagac atctgcaaaa tcgatgacat cctgcctgaa accggcgtct
    25501 gcgcgctgtt aggtgacgag caggtagcga ttttccgccc gtatcacagc gaccaggtgt
    25561 ttgcgatcag caacattgat ccgttcttcg agtccagcgt gctatcacgc gggctgattg
    25621 cggaacacca gggcgagctg tgggtcgcca gcccgctgaa aaaacagcgt tttcgcttaa
    25681 gtgatggctt gtgcatggaa gacgaacagt tttccgtcaa acattacgaa gcacgagtga
    25741 aagacggcgt ggtgcagctg cgcggttaat gttttaacgg gaggcgcaat gcctcccctt
    25801 tttgcatggt cctgtaatga ccttcggtat attgcaggac attttttaaa ctttttgttt
    25861 tattttttgt ttttattttt taaaggataa tcaaatgttt acagacacta ttaataagtg
    25921 tgcggctaat gctgcgcgca ttgcacgcct gtcggcaaat aacccgctcg gcttttgggt
    25981 cagctccgcc atggcgggcg catatgtggg tcttgggatc atcctgattt tcacgctcgg
    26041 taatttgctc gacccgtccg tacgtccgct ggtgatgggc gcgacctttg gtatcgcctt
    26101 aacgctggtg attatcgccg gttctgaact gttcaccgga cacaccatgt tcctcacctt
    26161 tggggtaaaa gcgggcagca tcagccacgg gcaaatgtgg gcaatcctgc cgcaaacctg
    26221 gctgggtaac ctggtcggtt ccgtcttcgt tgccatgctc tatagctggg gcggcggtag
    26281 cctgctgccg gtagatacca gcatcgttca ctccgtcgcg ctggctaaaa ccactgcacc
    26341 ggcaatggta ctgttcttca aaggtgcatt gtgtaactgg ctggtttgcc tggcaatctg
    26401 gatggcgctg cgcactgaag gggcggcgaa atttatcgct atctggtggt gtctgctggc
    26461 atttatcgcg tccggctacg agcactctat cgctaacatg acgctgttcg cgctctcctg
    26521 gttcggcaac cacagcgaag cctacacgct ggcgggtatt ggtcataacc tgctgtgggt
    26581 gacgctgggt aatactttat caggtgccgt attcatggga ttgggttatt ggtatgctac
    26641 gccgaaagcg aatcgtccgg ttgcggacaa atttaatcaa actgaaacgg ctgccggtta
    26701 attactaagg ggtttttacg tggatcattt gcctatattt tgccaattac gcgatcgcga
    26761 ctgtctgatt gtcggcggtg gtgatgtcgc ggaacgcaaa gcaaggttgc tgttagacgc
    26821 aggcgctcgc ttaacggtga atgcattagc gtttattcca cagttcaccg catgggcaga
    26881 tgcaggcatg ttaaccctcg tcgaagggcc atttgatgaa agccttctcg acacctgctg
    26941 gctggcgatt gcagcgacgg atgatgacac gcttaaccag cgcgtcagcg aagccgctga
    27001 agctcgtcgc atcttctgta acgtagtcga tgcgccgaaa gccgccagct ttattatgcc
    27061 gtcgattatt gaccgctcac cgctaatggt agcggtctcc tctggcggca cctctccggt
    27121 tctggcgcgc ctgttgcgcg aaaaactcga atcactgctg ccgttgcatc tgggccaggt
    27181 ggcgaaatat gccgggcaat tacgcgggcg agtgaaacaa cagttcgcca cgatgagtga
    27241 gcgtcgccgt ttctgggaga aattgttcgt taacgaccgc ctggcgcagt cgctggcaaa
    27301 caacgatcag aaagccatta ctgaaacgac cgaacagtta atcaacgaac cgctcgacca
    27361 tcgcggtgaa gtggtgctgg ttggtgcagg tccgggcgat gccgggctgc tgacgctgaa
    27421 aggactgcaa caaattcagc aggcagatgt ggtggtctac gaccgtctgg tttctgacga
    27481 tattatgaat ctggtacgcc gcgatgctga tcgcgttttc gtcggcaaac gcgcgggata
    27541 ccactgtgta ccgcaggaag agattaacca gatcctgctg cgggaagcgc aaaaaggcaa
    27601 acgcgtggtg cggctgaaag gtggcgatcc gtttattttt ggccgtggtg gcgaagagct
    27661 ggaaacactg tgcaacgcgg gtattccgtt ctcggtggtt ccgggtatta ccgcagcttc
    27721 tggttgctct gcctattcgg gtattccact cacgcatcgc gattatgccc agagcgtacg
    27781 cttaattacc ggacacttaa aaaccggtgg cgagctggac tgggaaaacc tggcggcaga
    27841 aaaacagacg ctggtgttct atatggggtt gaatcaggcc gcgactattc agcaaaagct
    27901 gattgaacac ggtatgcctg gcgaaatgcc ggtggcaatt gtcgaaaacg gaacggcagt
    27961 cacgcagcgc gtgattgacg gtacgctcac gcaactcggt gaacttgctc agcaaatgaa
    28021 cagtccatcg ctaattatta ttggtcgggt tgttggcctg cgcgataaac tgaactggtt
    28081 ctccaatcat taatttaacc cgggccagag aattctggcc ttcttaatga ctctttttat
    28141 atattcagca aataaaacat aaccccttaa taaatattat ctgctaatgg gttatattca
    28201 ccgttacagc gacttacaaa attaagtcat tcgctcactg acgccaatta aatattcctg
    28261 tatcgttctt aacgagcagt ctgcttatat tcatataatc aatgaatatt aattaatata
    28321 taatacatag ggaatgtaaa tgaacaaatt tattaaagtt gcactggtag gtgcagtact
    28381 ggctacgtta actgcatgta ctggtcatat tgaaaaccgt gataagaact gctcttacga
    28441 ctacctgctg cacccggcaa tttctatttc taaaatcatt ggcggttgcg gtcctactgc
    28501 acagtaagtc tctgacagaa atcggctaac accggtttaa ttgcccgacg ccctctggat
    28561 tttccgaggg cgtatttgtt tctatgtcct tcttgccccc gcccgtaaaa atattttcac
    28621 tttaaattca attttataac tacatccatt agccacagcg cgatcctgtg cgaaattttg
    28681 tgatcttcct ccacattaca caacatcata tgttgttata ttcatcatgc attgtcatgt
    28741 tgccttttaa atgactgcaa actctcccct acaacgtatt ggacaagaaa aaggtatcgc
    28801 tatgggaagc caggaactcc aacgcaagct cggattttgg gccgttcttg caatcgccgt
    28861 cgggacaacc gtcggctccg gtatttttgt atctgtgggt gaagtggcaa aagcagcggg
    28921 cacgccgtgg cttacggtgc tcgcgtttgt cattggcggg ttaattgtga tcccgcaaat
    28981 gtgcgtctat gcggaactat ccaccgctta tccggaaaat ggcgcagatt atgtttatct
    29041 gaaaaatgcc ggaagccgac cgctggcttt cctctccggc tgggccagct tctgggccaa
    29101 cgatgcgccg tcattgtcga ttatggcgct ggcgattgtc agcaatcttg gctttttaac
    29161 gcctatcgat ccgttgctcg gtaaatttat cgccgccgga ttaattatcg cctttatgtt
    29221 gctacacctg cgctccgttg aaggcggcgc agcgtttcag acgctaatta ccatcgccaa
    29281 aattatcccg ttcactatcg tcattggcct tgggatcttc tggtttaaag cggagaattt
    29341 tgccgccctt accaccactg cgattggcgc aacgggcagc tttatggcgc tgctggcggg
    29401 gatctctgcc accagttggt cgtataccgg catggcctct atctgttata tgaccggcga
    29461 aattaaaaac cccggaaaaa ccatgccacg agcgctgatt ggttcctgtc tgctggttct
    29521 ggtgctctac accctgctgg cgctggtgat ttccggcctg atgcccttcg acaaactcgc
    29581 caattctgaa acgccgattt ccgacgccct gacctagatc cccgcactcg gcagcaccgc
    29641 tgggatcttt gttgccatca cggcgatgat cgtcattctt ggttcgcttt ccagctgcgt
    29701 gatgtaccag ccgcggctgg aatacgcgat ggcgaaagac aacctgttct ttaaatgctt
    29761 cggccatgtg catccgaaat acaacacgcc ggatgtctcc atcatcctgc aaggggcgct
    29821 ggggatcttc ttcatcttcg tttccgatct caccagcctg ctgggttatt tcaccctggt
    29881 gatgtgtttc aaaaataccc tcaccttcgg ctccatcatc tggtgtcgta aacgcgacga
    29941 ttacaaaccg ctgtggcgta ctccggcttt cgggctgatg accaccctcg ccattgcgtc
    30001 aagcctcatt ctggtcgcct caacctttgt ctgggcaccg attcccggcc ttatctgcgc
    30061 cgtcatcgtt attgctactg gtctgcctgc ttacgccttc tgggcgaagc gtagccgcca
    30121 gctcaacgct ttgtcgtaat gttatctgaa gaaaataaaa tgttggatat tgataaaagc
    30181 accgtggact ttctggtcac cgaaaatatg gttcaggaag tggaaaaagt tctcagccat
    30241 gacgttccgc tggtgcacgc catcgtggaa gagatggtga agcgcgacat tgatcgtatt
    30301 tatttcgttg cctgcggctc gccactcaac gcggcgcaaa cggcgaaaca tctggcggat
    30361 cgcttttccg atcttcaggt ctacgccatt tccggctggg agttctgcga taacaccccg
    30421 tatcgcctcg acgatcgttg cgcggtgatt ggcgtttctg actacggtaa aacggaagag
    30481 gtaatcaaag cgctggagct gggccgggcc tgcggcgcac tcactgcggc gttcaccaaa
    30541 cgcgcggaca gcccgattac ctcggcggcg gaatttagca ttgattatca ggccgactgt
    30601 atctgggaaa ttcacctgct gctctgctac agcgtggtgc tggagatgat cacccgcctc
    30661 gcgccgaacg cggaaatcgg caagatcaaa aacgatctca agcagttgcc gaatgcgctc
    30721 ggtcatctgg tacgcacctg ggaagaaaaa ggccgccagc ttggtgaact ggccagccag
    30781 tggccgatga tttataccgt tgctgcgggt ccgctgcgtc cgctgggtta caaagaaggc
    30841 attgtaacgc tgatggaatt tacctggacg cacggctgcg tgattgagag cggagagttc
    30901 cgccatggcc cgctggagat tgtcgaaccg ggcgttccgt tcctgttcct gctcggcaat
    30961 gatgaaagtc gccacaccac cgaacgcgcc attaactttg ttaaacagcg tactgacaac
    31021 gtgatcgtca tcgattacgc cgaaatttcg caagggctgc acccgtggct ggcaccgttc
    31081 ctgatgttcg tgccaatgga gtggctctgc tactacctgt ctatttacaa agatcacaac
    31141 ccggatgaac gccgctatta cggtggtctg gtggaatatt aatccctctc ccggcccggc
    31201 aacgggccgg atttacgcaa ggagttaccc gatgaaaaca ggtatgttta cctgcggcca
    31261 ccagcggctg cctattgaac acgcatttcg tgatgcaagc gagctgggtt acgacggcat
    31321 cgaaatttgg ggcggtcgcc cgcacgcgtt cgcgccggac ttaaaagcgg gcggcatcaa
    31381 acaaatcaag gcgctggcgc agacgtatca gatgccgatt atcggttaca cgccagaaac
    31441 caacggctat ccgtataaca tgatgctggg cgatgaacat atgcgtcgtg aaagcctgga
    31501 catgatcaag ctggcgatat atatggcaaa agagatgaac gcgggttata cgctgatttc
    31561 tgcggcccac gcgggctatc tcacgccgcc taacgttatc tgggggcggc tggcagagaa
    31621 cttaagcgaa ctgtgtgagt acgcggaaaa catcggcatg gatctgatcc tcgaaccgtt
    31681 aacgccgtat gaatcgaacg tcgtgtgtaa tgctaatgat gtgcttcatg cgctggcgct
    31741 ggtgccttcg ccgcgcctgt tcagcatggt cgacatttgc gcgccgtatg tccaggcgga
    31801 accggtgatg agttatttcg acaaactggg cgataaatta cgtcatctgc atattgtcga
    31861 cagcgacggg gccagcgaca cgcattacat tcctggagaa ggcaaaatgc cgctgcggga
    31921 actgatgcgc gatattattg atcggggcta tgagggttac tgtacggtgg agctggtgac
    31981 gatgtatatg aacgagccca gactctatgc ccgccaggcg ctggaacgct ttcgcgcgct
    32041 gctgccggag gatgagagat gaaaacccta gcgacaatcg gcgataactg cgtcgatatc
    32101 tacccgcaac tgaataaagc gttttctggc ggtaatgcgg tcaatgtggc ggtgtactgc
    32161 actcgctacg gcatacagcc gggatgcatt acctgggtgg gtgacgatga ctacggcaca
    32221 aagctgaagc aggatctcgc ccgcatgggc gtcgatatca gccatgtcca tacgaaacac
    32281 ggcgttaccg cacaaactca ggtggaactg cacgacaatg atcgcgtttt tggcgactac
    32341 accgaaggcg tgatggccga ctttgccctg agtgaagagg attacgcctg gctggcgcag
    32401 tatgacattg tgcacgcggc aatctgggga catgcggaag acgcattccc acagctgcac
    32461 gctgcgggca aacttaccgc tttcgacttc tccgacaagt gggacagccc gctctggcag
    32521 acactggtgc cgcatctcga ttttgccttt gcctccgcac cgcaagaaga cgaagcgctg
    32581 cgtctgaaga tgaaagcgat tgttgcccgt ggcgcaggaa cagtgattgt cacgctgggt
    32641 gaaaacggca gcattgcctg ggatggcgcg cagttctggc gtcaggctcc tgaaccggtg
    32701 acggttatcg acaccatggg tgccggagat tcgttcattg ccggattcct ttgcggctgg
    32761 tctgcgggga tgacattacc gcaggcgatg gcgcagggaa cggcgtgcgc ggcgaaaacc
    32821 attcagtacc acggtgcctg gtaggtataa cgttggcgtg agcatcttca cgccaacgtg
    32881 ctgttacttg ccggaaaacg accctataat ccgagtaatt cattctttat ttcagggtcg
    32941 attatgtcag ctacggaccg ctactctcat caactcctct acgctaccgt ccgccagcgg
    33001 ctgctggatg atatcgcgca gggggtttac caggccgggc aacagatccc taccgaaaac
    33061 gagctttgta cacaatataa cgtcagccgc attaccattc gcaaagccat cagcgactta
    33121 gtggcagacg gcgtactgat ccgctggcag ggaaaaggca cctttgtaca aagccagaaa
    33181 gttgaaaacg ccctgcttac tgtcagtggt tttaccgatt ttggcgtctc acaaggcaag
    33241 gcgacgaaag agaaagtgat cgaacaggaa cgggtcagcg ccgcgccgtt ttgcgaaaag
    33301 ctgaacatcc ccggaaacag cgaagtgttc catctctgcc gggtgatgta tctcgataaa
    33361 gagccgctgt ttattgatag ttcatggatc ccgctgtcgc gttatcctga ctttgatgag
    33421 atttacgtcg aaggaagctc cacctatcag ttatttcagg agcgttttga cacgcgagtg
    33481 gtcagcgaca aaaagaccat cgatatcttt gccgccaccc gcccgcaggc aaaatggctg
    33541 aaatgcgaac tgggcgaacc gttgtttcgc atcagcaaaa tcgcctttga tcagaatgac
    33601 aaaccggtgc acgtctccga actcttctgc cgcgccaatc gcatcacctt aacgattgat
    33661 aataaaagac attaaccgta ggccggataa gatgcgccag catcgcatcc ggcgatgctg
    33721 gcgcactgaa ttttacatcc cgcacgttcc cctcacccta accctctccc caaaggggcg
    33781 aggggagcgt tcagtgcgga atttagcttg attgccgaac ggtgctatcc agcattaaat
    33841 actcgcaata ctctcacgca aaatccgcag caccgtctct tcaccgctgc gattcgggtt
    33901 aatgcgaatc gcacaatgtt cgagttgtgg attcgcctgg cgaaacgttc ctgaaagtcg
    33961 atagaagagc ggcgggattt catatttcga ctctgcaccg accgggtaag gcaacgcgcc
    34021 acgcttttgc gcttcttcca gcaccctggc ggcaatcagc tgatgaaact cgacaatcaa
    34081 caccttcgac tgcgcattgg caatgacggc gcttttcact tccgccaccg cgccgccgtt
    34141 aagcaaagcc agcaaccgtt cggatactcc cgcctgcacc gcatgcatca ctggcgcgaa
    34201 caccagacca cgcaagactt ccagcgcctg tgagccctgg atctggctac cgccggagta
    34261 aagcgtggcg cgaatccgac taataacatc ggcatcgcca accactgcac caacgccctc
    34321 cggcccaaac agcttgaagc aggagaatgt cgagacattc gcgccgcatt cacagccgat
    34381 tcgcgctacc ttcatcaccg catagttatc atcggttaac gctggaacac ctgccgcgcg
    34441 caacgttgcc agcacatctg ccagcacata gctgtcctgc ggctgctggc gcgtatgctg
    34501 caccagcgcg gcatcaggtt gttgctcgtc gacgacctgc ttcagtgcca acaggtcatt
    34561 gaaatcagca gtaataagcg tcagccccat ctgctcaata ataacccgtg tcgtcgggta
    34621 aacaggcgcg tcatgcacca gaagacgctg ccccggtttg agcaaagccg ccagcccggc
    34681 gcgaatcgcg ccagtccccg cgccctgcac cagcgccgcg gcctgtgcgt gaaatgcatc
    34741 agccagcacc tgctccaccc gttgggtaat acgcggttga ttcagacctg gagttaaccc
    34801 taaatcaccg ccagcaagaa actcgctgcc tggaaaatgg cggcaaatag tgtccaccag
    34861 cgcaaactgc ttttgctgcg cttcagcaag agtcaggctt tgcaaaggaa acgtcttcat
    34921 aggagccttc cttaagccgg aacgaacagg ccaagccagt aaagaacatt cagcagaata
    34981 ccggtgatca ttactgccac gaccggtgcc gccattttct gtaccggacg gcccagggat
    35041 tcgttgagga agtaaatagc aaccgcgata gagaatccgg tataacccgc catcttaatt
    35101 gctgcgaaaa tcgaaccgac cagcagcgcc atttccatca gcatattcat ggcgttacgg
    35161 atgttatccg acgcattacg caccgacggg tagcgtccca gccatttgcc gatcgaacga
    35221 agcagcaaga cttccgccga aataaccact gcgcctaata ccgctgcaac catcggattc
    35281 ggcgagagat agcccaccgc ataaacaaag gtaaagcccg caactgcata cacacccgtt
    35341 gctaaggcgg tggtggcaat caacggcaca aaccccagtc cgcgcataaa ttctgccaga
    35401 gccgcctgat taatcagcgt ttgcgattgt tccggcgtca cgcctgcgga atacgctttc
    35461 tccagtgtga agatcgaaac ttcgctacca gcaaaaatct tcatactggc aacggcggca
    35521 atcaatgctc ccacgatggc gatataaggt aagtttttga taatccgcga cgtgcgctct
    35581 tcaaacaccg aaagcccgct ggcatcgatg tcattttcat cacgatgacg caggtcgtga
    35641 gttatcgcga tccccagcag catcaccatg ccaataaaga tttcgatgga ttcagggtta
    35701 agatgtggga aatagcgcac gactaccaca cgggtcatca gaacaaccac ggcggcgacc
    35761 agactttgct tccagccaaa ctggtagaaa atcgccacca gcgggaacag tgcaaaagca
    35821 gaaaccaccg gcgagcttaa ttccccgagg ctacctaata catccaccgg cagcgcggtc
    35881 agcagctggt taactggcaa caggcaagta aggatcaaca cgccccagat agcgccaaga
    35941 ccaaacgcca tcaggctgtt tatcgccagt acgccgagga tatcggtagg aagaaagagt
    36001 aaccatgcgt tgagcagcca ggttttcagg gtaaaagaga tccccaccga ggccacaaaa
    36061 ccaatgctca gaccaaaagc gatactcccc gcctcgcgac ggttcatata gccttcaatc
    36121 agttgcggca ggatcgggtg gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga
    36181 agcgatgtca tacccgtcag gcacgccacc acgataatct gaatatacag atccatagtc
    36241 gtgcctttta tttcagatgg ttaatcaaca tcggaatcgc gtgttcgacg tgctcaacgg
    36301 aaaggccaaa cgccactttc ccttcagcga tcattttggc gatatgctcg tctttcgctt
    36361 tatgccgcgc ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc
    36421 accttcattg ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc
    36481 gcttcaatgg tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg
    36541 agtctgtcct tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc
    36601 ctgactgaat cctgactggc acaattgcgg aataaaggtg gtcaataaat agtcatagcc
    36661 ataaccacca ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg
    36721 gttcagcaac ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta
    36781 gtaactgttc ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa
    36841 aatgttgtcg aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac
    36901 tccgtgggct tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt
    36961 ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc
    37021 cagcggcgta atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc
    37081 cgtgccatcg ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct
    37141 gcgggtcacc acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac
    37201 gttgatcccg gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg
    37261 gttggtcatc tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca
    37321 aatgaacgca tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat
    37381 atgcagatgc tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct
    37441 gcaaaccgga gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat
    37501 tcacacacgg tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc
    37561 agcccttgct gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg
    37621 gtcggatcgt taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca
    37681 acaagccggg caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct
    37741 tctgcatgac cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg
    37801 gtgttaaatt cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag
    37861 ctcacaccat aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg
    37921 cccgcttcat acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc
    37981 acgacctgga agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc
    38041 ccttctttgc tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat
    38101 gtaatgaccc gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg
    38161 tcaaaagaga tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg
    38221 agattatcgc caatcgcaac cgcctgattg acccacagaa attgcagatc atcgccacgg
    38281 cgctccacct gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga
    38341 aaaggcatcc gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag
    38401 gtatcgccac cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc
    38461 atatcgcctg gcgcataacc caatgtgttg attagcccca gcttctccaa cgttggtagc
    38521 tgcaaatgcg gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcgga
    38581 cgcaccagcg tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca
    38641 aatcgcgcca tttatgcctt cagagggttg ccgaggctgt cataacgacc aactatttct
    38701 ggttcgccgc gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc
    38761 gtgcgaaaac agagcaccac tgcactgctc accggatact cgcctgccag cggcagggta
    38821 tagtcgatac tgctgtcatc cacggttttg agattggttt cagtaatctt ttgattttct
    38881 ggcgtaaaca ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag
    38941 tagctgtcgc cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca
    39001 ggctgatcgc cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca
    39061 tgagtcgcac cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg
    39121 ttcagttgct caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg
    39181 tgaagattcg gtgttggcaa aactttcccg gcagcctcat cccaaagcag gcaagggaaa
    39241 tgggttagtc cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc
    39301 agcgaatgct gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa
    39361 actttgagca gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgt
    39421 gctttgtcga gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc
    39481 tgatgacaag ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc
    39541 atgactcgcg cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat
    39601 ttttccgcca gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg
    39661 taaagccgcg ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc
    39721 acccagctgt ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc
    39781 gccgggttct ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg
    39841 aattaaatat tcattttttt aaatatttag gttgtgcgac gtggatttgt cgggagcttc
    39901 aaacctcttc gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt
    39961 cacacgggtt aacttccaga gcgaattcct tcaacaacac ctgatgcaat tgcacaacgg
    40021 cttgccagtg gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt
    40081 cttcaccacg gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt
    40141 gctcactgcg caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc
    40201 ctttgcagac gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca
    40261 tatcagatgc ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga
    40321 tgggcaagca gggcgtgttt atctacaacg gcacgcagca attgttgcat tgggtaatca
    40381 tcgactcgcg tcaggaccac ggcttcggtg gccagtaaaa atcgcggatc gtctgttagc
    40441 ggcgtcgcct ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc
    40501 gcatcgacat cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt
    40561 tccacatcac tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta
    40621 tcgagaccta ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca
    40681 ccaacatacg tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa
    40741 ctttccgccg ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc
    40801 acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct
    40861 ttcaatccgc tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc
    40921 acgttgttga tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg
    40981 ccattgcgcc cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg
    41041 gcctgcgtca ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt
    41101 cgattaccgc atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct
    41161 tttttgataa acgttcgacg cataatatat tttcaataaa atgaatattt atatcttcat
    41221 taaaatgaag ataagtggcg acgacgagat gtggaaacgg cgaggcgctt cacatttttt
    41281 cgggttgttg cgaaaagagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga
    41341 agtgatttat tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct
    41401 caccctaacc ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct
    41461 tcgccacaaa accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg
    41521 ctttttctgc gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt
    41581 gatagcgttc ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca
    41641 gatgaccata catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta
    41701 ccgcagaaag gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa
    41761 ctaccggcgg ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg
    41821 gatcttccag caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg
    41881 tcggctccag cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct
    41941 taaagatctc gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt
    42001 gctgtttctg gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag
    42061 ccatcagcac cggatagtca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt
    42121 tatctttaaa ctgcgtcatg cgactcagtt cgccgaagta ggtatagcag ttcagtgccc
    42181 agcctaactg cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat
    42241 cgataccaca agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg
    42301 catcctggcg cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat
    42361 cctgcatgtt tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg
    42421 agggctgtgc gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta
    42481 tgcggaagcc ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg
    42541 ctgagatcga tagcctcgcc gtagttatat ccgtaggtta agccaactga tgggcaacct
    42601 gccgcttttg ccgcctgaat atcattgcgt gagtcgccga caaacagcat ctgttgtggg
    42661 gcaattccca tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc
    42721 acatcatcgc cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc
    42781 ggcgcgacga acggcgtcgg tttgttggtg accaggccta gcggcaggcc tttagcctgc
    42841 aacgcgccca acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg
    42901 ccatagtagc gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg
    42961 tcatcaacgg gcggtttccc cattgtttta cgcagagtcg cacgttcctg acgcgcccag
    43021 gtcaatgcgc gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct
    43081 tcacctgcga cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagacca
    43141 ggagcactgt cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac
    43201 ttattcatga cttacctttg ccagttcact gcgcatttca tcaatgactt ttttatagtc
    43261 tggctggtcg aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc
    43321 gccaatgttg ttcaccttca cgccaccgtc cacttccaga cgaatgtcaa agccagactc
    43381 gtcgatacgg cgacgtactt cgcgcagttt atccagtgtt tgaggaatga aagactgacc
    43441 gccgaaacca gggttgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc
    43501 cagatagctc agaggtgtcg ccgggttaaa taccagaccc gctttacagc cattttcttt
    43561 aatcagttgc agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct
    43621 ggcaccagcg gcagcgaaat caggcacaat gcgatcgacg ggtttcacca tcagatgtac
    43681 gtcgataggg gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt
    43741 cagattggga acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc
    43801 cagggctttt gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc
    43861 aatcaaatac tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact
    43921 cctggtttgt acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt
    43981 atactgcgtc gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac
    44041 atcgtatcgt gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc
    44101 tccgccagat gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg
    44161 gcggtcgcag acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg
    44221 cgctccatgc tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg
    44281 aagtgataca actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta
    44341 aactcaccgc gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa
    44401 aacagtaccg cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag
    44461 taaacctcgg cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca
    44521 tcagtacgca tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg
    44581 atataacgag aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc
    44641 agacattcgc ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct
    44701 gcccacttca aaaaagcgcg atttttcttc atgccgacta actaattaca ccttctccgg
    44761 ctgtggagaa agctccgaca gcatccagcg cttcaggcag ctcccgcaac gataaaccat
    44821 tgcgggagat aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca
    44881 cgggtttttg gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt
    44941 cgaagcgtac acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata
    45001 gacaacgtag tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga
    45061 ggaactgctc agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt
    45121 acctgcggtc ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg
    45181 tactggagcc gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt
    45241 cgtgctcgcc acaggggcag ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg
    45301 ctccgttttc acggttgctt gcggtttttt cggttcaatc accgcctgct gacgaactgg
    45361 gcgcgtagtg gacggacgtt cagcggtttg cgttttcgcc gtgtcacgcg atgcattgcc
    45421 attgcgaaca ggcgcaaccg ttgcgggttc agtcggcaac gtggaattga ccgccacatt
    45481 gttcagctgt tgctgatttt gtggctgggt cagcgcattg ttcaggtcac cctgcacttc
    45541 aacacgttgt tgaccatccg ttgccaccgg ggtttgccct tgagtcggcg tagaagagat
    45601 cggtggcaga gaaacatcct gctgagtatt ttccgcagac gtggttcccg gcgctggctg
    45661 cacgccattc gcctgatcgg tcgcattgcc agcaagatca atactcttct cgccagacgc
    45721 ggtttgatcg ctggaagtgg tcgagggggc ttttagcgca gaaccgatac cgatgatcaa
    45781 cagcagtaga accagaatgc cgacgcccat catcatatac tgacgagaag cgggtttact
    45841 ggctgctttt ttgcgcttac gcggacgacg ctctacgcgc tcttcatcca cggtttcatc
    45901 ttcggattcg tcaatttctt cttcgatttc cggttcctca ttgcgctctt tttgcgcacg
    45961 agtcggacgg cgatcgtcag tgtcatcaag ttcaatatca tcaaaattga tctgcggttc
    46021 gccacgttca gtacgctcag aacgttcaga agattgacga gaacgaccag tacgacgatc
    46081 gctgggatcg ggtttcagct cgtcttctgg tttgaattca tccatttaac accccactaa
    46141 aaggttaatg cttaccacgt tgcaattaac ctgaagctaa tagaccgctt gataagcggc
    46201 ctgacctttc ttgttgttac gctgattgac aatcggcaat ggcgttaaga acaagctcgt
    46261 gcgaaacgcc gctgcgaact tcactcttac caattgccaa cggaagaatt aagcgcatct
    46321 ctcccgcaag gactttcttg tcacgcagca tatgcggtaa atacgcctgc gcggacattt
    46381 cgcgcggccc attgaccggt aacccagccc gcgtgagcag ggttataata cgctgcgttt
    46441 cggcagaact aaactgcccg agacgttccg acgtccgcgc cgccatcacc atacccgcag
    46501 cgaccgcttc accatgtaac caattgccat accccatttc agcttcaatg gcatgaccaa
    46561 aggtgtgtcc cagattcagt aaagcacgta acccggtttc gcgctcgtcg gcagcgacaa
    46621 cttctgcctt cagttcacaa caacggcgaa tacagtacgc cattgccgga ccgtccagac
    46681 gcaacaacgc atccagattc tcttccagcc agttaaaaaa cgcaccgtca agaataatgc
    46741 cgtatttgat gacttctgcc agccccgacg ctaactcacg cgggggaagc gttttcagac
    46801 agtcgagatc caccaccact gaagcaggct ggtagaacgc gccaatcatg tttttaccga
    46861 ggggatggtt gaccgcagtt ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg
    46921 tcgggacttg aatgaaacga acaccgcgct gataactcgc cgccgcgaag ccggtcagat
    46981 cgcccactac gccgccgcca agcgccacca gcgtagtatc gcgaccatgc ggcttttgta
    47041 acaacgccgt aaagacggta tcgagtacag ctaggctttt atactgctcg ccgtcaggga
    47101 ggataacgct atcgacgtta acacccgcct gttcaagtac gccgcggacc ttatcgagat
    47161 acagaggagc cagggtttcg ctggtgacca acatgacctg ctcgcccgat ttcagcggta
    47221 agaatgaagc tggttcatta aacaaaccag atgcgatggt aattgggtaa ctacgttccc
    47281 cgagagtaac gacaatcctc tccataacgc gacatccacc ttaattactg tacccgcaga
    47341 cgagtgtata taaagccaga attagttgct ttccagcatg tgaataatct ggtttgcaac
    47401 cactttagcg ctttgatcat cagtacgaat ggtcacgtcg gcaatctctt catacagcgg
    47461 attgcgttca ttggccaacg cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa
    47521 cgggcgtttt ttatcacgct gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata
    47581 aacgacaacg ccacgagcgg aaagacggtt acgcgtttca cgggatttca cagagccgcc
    47641 gccagtagcc agcacaatac cctgtttctc ggtcaactca ttgatgacct tttcttcgcg
    47701 atcgcggaag ccttcttcgc cttctaaatc gaaaacccag cccacatcag ctccggttcg
    47761 tttctcaatc tcttgatcgg aatcgtaaaa ttccatattg agtttttgag ctaactggcg
    47821 cccaatagtg ctttttccgg cacccatagg cccaaccaga aagatattgc gtttctctgc
    47881 cattttttcg gtactactaa gactattcgt taatgataaa cccgcttcgc tcagagagcg
    47941 ccgcaggaca tgaactgaaa cctcataaga tattgcgaga gtcagactga aaattatctc
    48001 aatactccag cgggtttggc aactgaataa atcacccatc cctctgcata tctggtcgct
    48061 gcaagcgcac tgccttgcta ccaccgctct ggcgataaat caccgggtaa gattagcgta
    48121 aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa
    48181 gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga
    48241 tgaaacacgt tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca
    48301 gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa
    48361 ataattgccc gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat
    48421 ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gctttttacc tcgacctgcg
    48481 tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc
    48541 ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg
    48601 gcgtgacctc catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc
    48661 cgctggaaac ctgatatgga atttcgctcc cctgtttaat gctggcaggc tgaagatgtg
    48721 aggccagcag acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa
    48781 gctcaagatc cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg
    48841 ccgtcgctac ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt
    48901 gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga
    48961 caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt
    49021 caagcgcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga
    49081 cggtcatact ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt
    49141 cggcgtattg cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct
    49201 gctccgcctc ctggcgggcg atattgtcat tctgccaggc aatggaatgc actgagagaa
    49261 tgttgccttc ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct
    49321 tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca
    49381 ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca
    49441 ccatcagcgt cactttttgc ggctttgccg cctgtacgcc ggatatcagc atcaacagta
    49501 gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt
    49561 tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag
    49621 ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt
    49681 acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg
    49741 ctaatccggc atagatcttc cggcgattta aaagggtcac gcataccggt taaaaggcac
    49801 aatgcaatac ctgccaacaa ccagcgttta accctcatgc ggcatctcca gttgtagcgt
    49861 gaacaaaaga tcatcacctt ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc
    49921 tgccagccgt gtaaatgccg acggcactgc ttcccacagc gttttcaacg ccaactcgcc
    49981 tccctgcgcg gatggatgcc aggaaaccag ttgcgcgccg gataactgaa aatccagtgg
    50041 cgaaaagggc agcgtttttt cctcgctgaa gggagtggtg tctaccaggc gatacagtgc
    50101 ggcccactgc tggtgatgac ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg
    50161 tgtcgatgat agaaaaatga gcgtaactaa catcagcaac cagaatgccc agcaaaactg
    50221 gcggaggcgg ggtgatgtgg cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc
    50281 ttgttaactg atactcaaat tgccagcgtc cctgcgcatc ctgctgcgtg gctccccgct
    50341 gattgagatg aaaagacgca tcctggcgga gtgacgtttc tagtgcgttt aacgcggtaa
    50401 tgcttgttgt cagccccttg atctccagcg ttccctgctg ccagcttatc gttgtcagcc
    50461 aggcgtgctc gggtaaaagc gccgccagtg cttccagcgc agattgccag tcgcgggtaa
    50521 attgtcgctg gcgctgccgt tgcgaacgct gttcgcgtaa ctgctgttgc tccagcaaac
    50581 gtggcttcgt tatctgtaag ctgcgggcga gttgttgttc cgcctgaagc aaaactgcgt
    50641 ctatgcgcgc ttcggcgctg cctgtcagac gcagtattag cgttatcccg acggccagca
    50701 gcagaggcgc aacgaacatc agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc
    50761 gccagggcaa aaaattaatt ggcgggttca tcagtacgcc ttcccaagcg ccagccccag
    50821 cgcgatggca aagtctccac caggcggcgg tagcggcggc tgacgaacag aaacggcctc
    50881 ccaggggtca aatccgcctt cgccacatat cgcgacgctg tcgggatcaa cggataacgc
    50941 tgccgccagc tccttcgcgc tagtcatccc caccgccagt ttgcgccccc agctatagcg
    51001 cgtcgcccac agccactgtt cgttatcacg ccaggccaga cattgctgat gagaaggtaa
    51061 aaaaggcagg aatcgctgta atgcactggc atccggggtg atcgcactaa catgaacacg
    51121 caacctttct gccagcgtaa gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt
    51181 ataagcgggg ctgagtgagt cttcgctgta atcgaagcgc agggagtccg gatccatatc
    51241 cagctcgcgg gccatcgtcc ctgacagcca ggccgtttgc tcccgctcac caagggacat
    51301 cgacgggcgc ggaaatgacc gctgtaatgt gcgactggcg ggaaacgcca acataatgtg
    51361 atgacgctgc ggcagttcgc gactccacgg taacaacgtt ttagccagct gctgcgcatc
    51421 aacaatccgc ccatctttga taatgtcgtg cgccagcggc aaccgccacc agcgttgcaa
    51481 aaagcattct tttgcgcccc gcacgatcgc aaccgctgcc acttcttgct gttgtaaatg
    51541 caaaccaatt tgccagatct taaatgtcat tgtgatgatc tccttatcac ccgtcactct
    51601 gacgggtata tcaatgcgtc tggcttgcct ttatactacc gcgcgtttgt ttataaactg
    51661 cccaaatgaa actaaatggg aaatttccag tgaagttcgt aaagtatttt ttgatccttg
    51721 cagtctgttg cattctgctg ggagcaggct cgatttatgg cctataccgc tacatcgagc
    51781 cacaactgcc ggatgtggtg acattaaaag atgttcgcct gcaaattccg atgcagattt
    51841 acagcgccga tggcgagctg attgctcaat acggtgagaa acgtcgtatt ccggttacgt
    51901 tggatcaaat cccaccggag atggtgaaag cctttatcgc gacggaagac agccgcttct
    51961 acgagcatca cggcgttgac ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct
    52021 ccggccacgc gtcacaaggg gcgagtacca ttacccagca gctggcgaga aatttcttcc
    52081 tcagtccaga acgcacgctg atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg
    52141 aacagttgct gacgaaagac gaaatcctcg agctttatct gaacaagatt taccttggtt
    52201 accgcgccta tggtgtcggt gctgcggcac aagtctattt cggtaaaacg gtcgaccaac
    52261 tgacgctgaa cgaaatggcg gtgatagccg ggttgccgaa agcgccttcc acctttaatc
    52321 cgctctactc aatggatcgt gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg
    52381 atgaagggta tatcacccag caacagttcg atcagacacg cactgaggcg attaacgcta
    52441 attatcacgc gccggagatt gctttctctg cgccgtacct gagcgaaatg gtgcgccagg
    52501 agatgtataa ccgttatggc gaaagtgcct atgaagacgg ttatcgcatt tacaccacca
    52561 tcacccgcaa agtgcagcag gccgcgcagc aggcggtacg taataacgtg ctggactacg
    52621 acatgcgcca cggctatcgc ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt
    52681 gggataacaa caagattacc gatacgctga aggcgctgcc gacctatggt ccgctgctgc
    52741 ctgccgcagt caccagcgcc aatcctcagg gagcgacggc gatgctggcg gacgggtcga
    52801 ccgtcgcatt gagtatggaa ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc
    52861 agggaccgac gccgcgtaaa gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc
    52921 gtcaggttgg cgatgcatgg tggctggcac aagtgccgga agtgaactcg gcgctggtgt
    52981 cgatcaatcc gcaaaatggt gccgttatgg cgctggtcgg tggctttgat ttcaatcaga
    53041 gcaagtttaa ccgcgccacc caggcactgc gtcaggtggg ttccaacatc aaaccgttcc
    53101 tctacaccgc ggcgatggat aaaggtctga cgctggcaag tatgttgaac gatgtgccaa
    53161 tttctcgctg ggatgcaggt gccggttctg actggcagcc gaagaactca ccaccgcagt
    53221 atgctggtcc aattcgctta cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac
    53281 gtgcaatgcg ggcgatgggc gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc
    53341 cggcgcaaaa cattgtccac accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa
    53401 tgcaggtggc gcgcggctac gcggtcatgg cgaacggcgg cttcctagtg gacccgtggt
    53461 ttatcagcaa aattgaaaac gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag
    53521 cctgcccgga atgcgatatt ccagtgattt acggtgatac gcagaaatcg aacgtgctgg
    53581 aaaataacga tgttgaagat gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa
    53641 tgccgcagct ggagcaggca aatcaggcgt tagtggcgaa gactggcgcg caggagtacg
    53701 caccgcacgt catcaacact ccgctggcat tcctgattaa gagtgctttg aacaccaata
    53761 tctttggtga accaggctgg cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc
    53821 gcgatatcgg cgggaaaacc ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt
    53881 acggtccggg cgttgtgacc tcggtctgga ttggctttga tgatcaccgt cgtaatctcg
    53941 gtcatacaac ggcttccgga gcgattaaag atcagatctc aggttacgaa ggcggtgcca
    54001 agagtgccca gcctgcatgg gacgcttata tgaaagccgt tcttgaaggt gtgccggagc
    54061 agccgctgac gccgcctccg ggtattgtga cggtgaatat cgatcgcagc accgggcagt
    54121 tagctaatgg tggcaacagc cgcgaagagt atttcatcga aggtacgcag ccgacacaac
    54181 aggcggtgca cgaggtggga acgaccatta tcgataatgg tgaggcacag gaattgttct
    54241 gattaaaaag gcgcttcggc gccttttcag ttggctgaca aagtgcattt gtttatgccg
    54301 gatgcgggtg aacgcgttat ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa
    54361 ccccataagc ctgataagcg ttgtgcatca ggcaaacttc acgcatttac actcgcccct
    54421 gccctttcaa ccattcgcgc acgaggaaca gcgcgctgac attacgcgct tcgttgaagt
    54481 cagggtcttc cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt ggtagcggct
    54541 ctggctcatc gccttccagc gattccgggt agagatcttg cgcgaccacg atattcattt
    54601 tactggaaaa gtaagacggt gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac
    54661 caaatccaac ctcttctttt agctcgcggt tagcggcttc gtagacgctt tcacccggat
    54721 caattaatcc tttcgaaaaa cctaattcgt aggattcagt tcccactgcg tattcgcgga
    54781 tcaggatcag gtgatcgtcc acaatcggca caatcatcac tgcttcccgg ttggttggac
    54841 gcattcgttc ataaacacgc cgcacgccat tgctgaactc cagatccaca ctctcgacgg
    54901 taaacagtcg ggaacgggct acagtttcaa cattcagaat ggtgggtttt tgtaatgatt
    54961 tgttcatcgt gggatctatg ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt
    55021 tcggcaatgt gaattgcatg ttatttacat ttatgtaact taataaataa tcgtcctcaa
    55081 atcaaattaa aagtcaatag gttgaaataa ctccaggaat ttgctgatat tccgccttca
    55141 gagggtttgc tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc
    55201 acgcttgccg atagccaacc gcagaatcat gtattgtgtc cggtgcgact gaccacgcct
    55261 gacagactaa gtaagatggg gaaagcatga gcaccattgt gattttttta gctgctttgc
    55321 tggcctgctc actacttgcg ggatggctga taaaagtgcg atccagacgg cgtcagctgc
    55381 cctggaccaa cgccttcgcg gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg
    55441 ccgttgaaaa ttatcttgag agcctgacgc aggtattaca ggtgcctggc ccaacgggag
    55501 ctagcgcggc accgatctct ctggcgctga atgccgaaag caacaacgtc atgatgctga
    55561 cacacgctat cacacgttac ggcatctcta ccgacgatcc gaataaatgg cgttactacc
    55621 tcgattcggt agaagtccac ctgcccccct tctgggaaca gtacatcaac gatgagaata
    55681 ccgttgaact gattcatacc gattcgctgc cgttggttat ttcactcaac ggtcatacgc
    55741 tgcaggagta catgcaggaa acgcgcggct atgccttgca acctgttccg tccacgcagg
    55801 cgtcgattcg cggggaagag agtgagcaaa tcgagctact caatattcgc aaagaaacgc
    55861 atgaagaata tgcgctgagt cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct
    55921 ccttcctgat gttctttttc tgcctgatta ccccggatgt atttgttccg tggctggcag
    55981 gcggcgcatt actgctgctg ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat
    56041 cctccctgcg agaaattcat tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg
    56101 aaaacgatca ggaacagatc aacaatattt cactcggtat tatcgacctg gtctatcccg
    56161 cacactggca gccatacatt gctcaggatc tcggtcaaca aaccgatatt gatatctatc
    56221 tcgaccgcca cgtagtgcgt caggggcgat atctttcgct gcatgatgaa gtaaaaaact
    56281 ttccgttaca gcactggctg cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt
    56341 ttatgctgtt attctggatc ccgctggata tgccgctgaa attcactctc tcatggatga
    56401 aaggcgcgca gaccattgaa gccaccagcg taaaacaact ggctgacgct ggcgtgcggg
    56461 taggcgatac attgcgtatt agcggtacgg gaatgtgtaa tattcgcact tccggaacct
    56521 ggagcgcgaa aaccaattca ccttttttac catttgactg ctcgcagatc atctggaatg
    56581 acgcccgctc attaccatta ccagaatctg aactggtcaa caaagcgacg gcattgactg
    56641 aagcagttaa tcgccagctg caccctaaac cggaagatga atctcgcgtc agtgcctcat
    56701 tacgttcagc gattcaaaaa tccggcatgg tattgcttga tgattttggc gacattgtac
    56761 tgaagacagc ggatttatgt tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg
    56821 tcaatctcgg caacagtaaa gactgggacg cgctggtaaa acgcgccaac gctgggaagc
    56881 tcgatggcgt gaatgtgtta ttacgcccgg tgagtgcgga atcgctggat aacctggtgg
    56941 caacctccac cgcgccgttt atcacgcatg aaacggcgcg agcggcacaa tcactaaaca
    57001 gtccggcccc cggcggattc ctgattgtca gcgacgaagg cagcgatttt gttgatcagc
    57061 cctggccttc ggcatcactt tacgactacc cgccgcaaga acagtggaac gctttccaga
    57121 aactggcaca aatgctgatg catacgccgt ttaacgccga aggtatcgtc acaaaaatct
    57181 tcactgacgc caatggtacg cagcatattg gccttcatcc gatcccggat cgttccggcc
    57241 tgtggcgcta tctcagcacc acattgctgc tactgacgat gctgggtagc gccatttaca
    57301 atggcgtaca ggcctggcgt cgttaccagc gtcatcgcac tcgcatgatg gagattcagg
    57361 cctattatga aagctgcctg aacccgcaac tgatcacccc ttcagaaagc cttatcgaat
    57421 aacacgtttg cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac tctggagatt
    57481 tccctcatgc atatcaacat tgcctggcag gacgtagata ccgttctgct ggatatggac
    57541 ggcacgttgc tcgacctcgc cttcgataac tatttctggc aaaagctggt gcctgaaaca
    57601 tggggcgcga aaaacggggt tacgccacag gaagcgatgg aatatatgcg ccagcaatat
    57661 cacgacgtac agcatacgct aaactggtac tgtcttgatt actggagtga gcaactgggt
    57721 ctggatatct gtgcgatgac caccgagatg ggaccgcgcg ccgtactgcg tgaagatacc
    57781 attccgtttc ttgaggcact gaaagccagc ggtaagcagc gaattttgct caccaatgct
    57841 catccgcaca acctggcggt aaaacttgag catacgggtc tggacgcaca ccttgattta
    57901 ttactttcca cccacacatt tggttatccg aaagaggatc agcggttatg gcatgcggtg
    57961 gccgaagcta cgggtctgaa agctgaaaga acgctgttta ttgatgacag cgaagcgatt
    58021 ctcgatgctg ccgcgcaatt tggtattcgt tactgcctcg gcgtgactaa tcctgattcc
    58081 gggattgccg agaaacagta tcaacgccat ccgtcactga atgactaccg ccgcctgatc
    58141 ccctcgctaa tgtgaaggag acgccatgaa agagaaacct gctgttgagg ttcgactgga
    58201 taaatggcta tgggctgccc gtttttataa aacccgcgcg ctggcccgtg aaatgattaa
    58261 aggcggtaag gtgcattaca acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa
    58321 tgccacgctc actctgcgcc agggaaatga cgaacgcacg gtgattgtaa aggcgattac
    58381 tgaacagcgt cgccccgcca gcgaggcagc cttgctgtat gaagagactg cggaaagtgt
    58441 agagaaacgc gaaaaaatgg cgatggcacg taaacttaat gccttaacca tgccgcaccc
    58501 ggaccgacgc ccggacaaaa aagagcgccg cgacctgtta cgatttaaac acggcgacag
    58561 tgaataactg tcacctgcaa gagagatgat tatgccgcaa catgaccaat tacatcgcta
    58621 tctgtttgaa aattttgccg ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca
    58681 gatccttgag aaccacgatt atccgcagcc cgttaaaaac gtgctggcag aactgctggt
    58741 tgcgaccagc ctgttaaccg ctacgctgaa gtttgatggt gatatcaccg tacagctgca
    58801 gggcgacggt ccgatgaatc tggcggttat taacggtaac aataaccagc aaatgcgcgg
    58861 tgtggcgcgc gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa
    58921 tggttacgtg gtgatcacca ttaccccgag cgaaggcgaa cgctatcagg gcgtagttgg
    58981 tctggaaggt gataccctgg cggcctgcct agaagattac tttatgcgtt ctgaacagct
    59041 gccgacgcgc ctgtttattc gcaccggcga cgtagacggc aaaccggctg caggcggtat
    59101 gttgttgcag gtaatgcctg cgcaaaatac ccagcaggac gactttgacc acctggcgac
    59161 gctaaccgaa accatcaaaa ccgaagaact gctgacctta ccggcaaacg aagtgttgtg
    59221 gcgtttgtat cacgaagaag aggtgacggt ttacgatccg caggatgtgg agttcaaatg
    59281 cacctgctcg cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga
    59341 tagcatcctg gcggaagatg gcgaaattga catgcattgt gattactgcg gtaaccacta
    59401 tctgttcaat gcgatggata ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca
    59461 agttcattaa tgagtatgtc cggcagagag ggctctgccg gattttacga cttaatggcc
    59521 tgatgtgctg cgcttaccag cgcctatgaa ataaatcgca aattattttg gtcagataca
    59581 gcgtcgtatc cgataaaagc gttgtcactt acttaagttt atttccagcg tttcaccatc
    59641 taccgtcaga tcgcgccacg ctttcggcct cgtccccagt tttccatcct tcgcagctgt
    59701 gagtaatttt tctttcgtta tttcgttcgg aagtaaactc atatcaagcg cgtcccattc
    59761 ttttctgtct gagtcaaagc catagacaat gactctgtca tcattaaaag caaacaggat
    59821 ccgctccgcc tgaccatcac tattcagatc ttgctcgacc agtacgcagg cgtctttttc
    59881 aatacaggtc atcacgttat agcgatcctg gattaaggcc gaccagaatg tcgcatccgg
    59941 tttaacagaa ccaggggcaa ttaacacatt ttcggctaat actttttccg atacctgttc
    60001 ctgaagatgt tgctctccat ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt
    60061 cataaacccg gcatcgcttt tcagcgactc aagcgccgca cgtccatagc gaccgctctg
    60121 ttcgagcatg tagatagtta cctggtctgg cgtgtttttg ccgctctgat aacgcgccat
    60181 atgactgttc acgctaatac gcatactgtc cagcaccggc gaattaagaa gcaccagtat
    60241 caccaacacc aataacgaaa ccgcaaggtt cactttgccc tgaagaacaa ggggattttg
    60301 cccattacgc cagacgatac tgacaaaata ccccagcgac cacaccagta acaccagctc
    60361 cgccagcgcg ccctgcaagc ggtcgacagt ccagccgtac tgagcgaccc gcagccataa
    60421 cccccaggtg gcgacgaaca catacagcgg agcaaccagc aaagcggttt taatcaggca
    60481 acgcaacggc cccgtccagg gaagtgacgc tttttgcgga tcgcggacaa tagccattaa
    60541 gatcaattgc aaaaaggcca gcgttaacag caacccggcg gcggagatgt ggcgagaaat
    60601 cgcgctcagg cccgtaaacg gcagggtgat gataaacatc agggttagca atgatactaa
    60661 cggcagcaac cccgtggcga ttagcgtgaa caacttttga atagagtcga ttaaacgtga
    60721 ctgtgtacgc gcgaggatca ccgccagtgc ggtaaccaga ccaaatgtaa gatacataaa
    60781 ccagtcggtt gcaaaaaaga gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc
    60841 accccacagt aaaagcacca gccacgtcag gccattggag agaaaaatca ccagtaatat
    60901 aagtacgtta tgccatactg actggtaaaa gtagctatag cggctactgc cattgcggat
    60961 acgaaggctt tgttgtatcc acggtagcaa caacattgcc atcaacagca gatagcagcc
    61021 aaaatcccaa atcgcctttt cagctctcca ggggttcatg ccatcagttt gccacttcag
    61081 ccaaccgctc atccctgtcg tggcaataaa caccagcgcc agccatcccc aaaggcgctt
    61141 ctgtttaaaa gagatcacgg agaagagtaa aacggatgaa aacgcgacgg tcgcaggcac
    61201 gccataaaca atccaactgt gattttttcc ggcaagccag gcgataagca ggtagcaaac
    61261 cagcccctga agtaatccgg tagcaatcat cccccagcgt gttgccggtg aaagttcaac
    61321 gttatccatg ttggttatcc agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag
    61381 tattttgctt ttttcagaaa ataatcaaaa aaagttagcg tggtgaatcg atactttacc
    61441 ggttgaattt gcatcaattt cattcaggaa tgcgattcca ctcacaatat tcccgccata
    61501 taaaccaaga tttaaccttt tgagaacatt ttccacacct aaaatgctat ttctgcgata
    61561 atagcaaccg tttcgtgaca ggaatcacgg agttttttgt caaatatgaa tttctccaga
    61621 tacgtaaatc tatgagcctt gtcgcggtta acacccccaa aaagacttta ctattcaggc
    61681 aatacatatt ggctaaggag cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa
    61741 ctcgaggctt atggtatcag tgacgtacat gatatcgttt acaacccaag ctacgacctg
    61801 ctgtatcagg aagagctcga tccgagcctg acaggttatg agcgcggggt gttaactaat
    61861 ctgggtgccg ttgccgtcga taccgggatc ttcaccggtc gttcaccaaa agataagtat
    61921 atcgtccgtg acgataccac tcgcgatact ttctggtggg cagacaaagg caaaggtaag
    61981 aacgacaaca aacctctctc tccggaaacc tggcagcatc tgaaaggcct ggtgaccagg
    62041 cagctttccg gcaaacgtct gttcgttgtc gacgctttct gtggtgcgaa cccggatact
    62101 cgtctttccg tccgtttcat caccgaagtg gcctggcagg cgcattttgt caaaaacatg
    62161 tttattcgcc cgagcgatga agagctggca ggtttcaaac cagactttat cgttatgaac
    62221 ggcgcgaagt gcactaaccc gcagtggaaa gaacagggtc tgaactccga aaacttcgtg
    62281 gcgtttaacc tgaccgagcg catgcagctg attggcggca cctggtacgg cggcgaaatg
    62341 aagaaaggga tgttctcgat gatgaactac ctgctgccgc tgaaaggtat cgcttctatg
    62401 cactgctccg ccaacgttgg cgagaaaggc gatgttgcgg tgttcttcgg cctttccggc
    62461 accggtaaaa ccaccctttc caccgacccg aaacgccgcc tgattggcga tgacgaacac
    62521 ggctgggacg atgacggcgt gtttaatttc gaaggcggct gctacgcaaa aactatcaag
    62581 ctgtcgaaag aagcggaacc tgaaatctac aacgctatcc gtcgtgatgc gctgctggaa
    62641 aacgtcaccg tgcgtgaaga tggcactatc gactttgatg atggttcaaa aaccgagaac
    62701 acccgcgttt cttatccaat ctatcacatc gataacatcg ttaagccggt ttccaaagcg
    62761 ggccacgcga ctaaggttat cttcctgact gctgatgctt tcggcgtgtt gccgccggtt
    62821 tctcgcctga ctgccgatca aacccagtat cacttcctct ctggcttcac cgccaaactg
    62881 gccggtactg agcgtggcat caccgaaccg acgccaacct tctccgcttg cttcggcgcg
    62941 gcgttcctgt cgctgcaccc gactcagtac gcagaagtgc tggtgaaacg tatgcaggcg
    63001 gcgggcgcgc aggcttatct ggttaacact ggctggaacg gcactggcaa acgtatctcg
    63061 attaaagata cccgcgccat tatcgacgcc atcctcaacg gttcactgga taatgcagaa
    63121 accttcactc tgccgatgtt taacctggcg atcccaaccg aactgccggg cgtagacacg
    63181 aagattctcg atccgcgtaa cacctacgct tctccggaac agtggcagga aaaagccgaa
    63241 accctggcga aactgtttat cgacaacttc gataaataca ccgacacccc tgcgggtgcc
    63301 gcgctggtag cggctggtcc gaaactgtaa tgatttgaag ctggagaata tctatccagt
    63361 atcttataga aagcaaaacg ggaggcacct tcgcctcccg tttatttacc cttcttttgt
    63421 cgtgccctgc gcccgcgtta ccggcactgg cagccaggcg cgaatggaaa gcccgccccg
    63481 ctcgctggtg ccaagctcca gcatcccgtt atggttatcc acgatacgct gcacaattgc
    63541 cagccctaat cccgtgccgc taatggtgcg cgcactgtcg ccgcggacaa acggctggaa
    63601 caggtgctta cgttgttccg gcgcaattcc cggaccgtca tcttccacct ggaaccaggc
    63661 gcgattcggc tccgttccgc tgctgacttt gatccagcca ttgccataac gggcggcgtt
    63721 gaccaccata ttcgccaccg cgcgtttgat cgacagcggg tgcattttca cttcaatgct
    63781 gccggggtaa agcgcggttt caatttcccg ctcatagcca ctttcggcag caatcacctc
    63841 accgagcact gcattaagat ccgccatttc catcggcatc tcctgcccgg tgcgcaggta
    63901 gtcgataaac tgctcaatga tggcgttgca ctcttcgata tctttattga tcgattctgc
    63961 cagatagcca tcctgctcgc tcatcatctc agtcgccagg cgaatacgcg tcagcggcgt
    64021 gcgcaagtcg tgacttaccc ccgccatcag cagcgtgcgg tcatccgcca gttgcttaac
    64081 accagccgcc atatggttaa aggcacgggt aacggaacgc acctccgaag cgccatattc
    64141 acgcagcggc ggcggaataa tccctttacc aacctgcaag gctgcgtgtt cgagatcgac
    64201 caacggtcgg ttctggatac gaataaacag ccacgccccg cctatcgcca atagcataat
    64261 cgccagcgta tagcggaaca gcggagagaa atcgccctga tgaatttcgg tcagcggcac
    64321 gcgtacccag atattgggcg acagccaggt tttcagccag acgacaggcg aacttttgtt
    64381 gacctcaacg cgcacttccg tcgggccgcc cagttgctgt gccatctgat ggcttaagaa
    64441 ttcatagtgt tgcgcccaac gcagacctgc ctcttcggca gcctcgttgg agtagagaga
    64501 gatccccagc tcacggtaga tctcccgacg gaaagcggga ggcacaacca actgcgtgcc
    64561 gtcctccagt tgcagtttat cggtcatcaa catacgcact tcgtacgcga ggactttatt
    64621 aaactgctgg aggctcggca aaatcgcgaa gttcagcacc accagataag tcgtcaccag
    64681 gctggcgaac agcaaggtga cgatgagcaa taacgtacgg gcaaatgaac ttcgtggcga
    64741 gaagcgcaat cgcctcatgc tttagagccg tccggtacaa agacgtagcc caggccccaa
    64801 acggtctgaa tgtaacgcgg atgcgctgga tcttcttcta ccatgcggcg cagacgcgaa
    64861 atctgcacgt cgatggagcg ttccattgcg gaatattcac gaccacgggc aaggttcatc
    64921 agcttatcgc gggagagcgg ctcacgcgga tggctgacca gtgccttcag taccgcaaac
    64981 tcaccgctgg tgagcggcat cggctcatct tcgcggaaca tttcgcgcgt accgaggtta
    65041 agtttgaact taccgaaagc aattaccgcc tcttcctgtg acggtgcgcc tggcagttcg
    65101 ttcgcctgac gacgcagcac cgcacgaata cgggccagca gttcacgcgg gttaaacggt
    65161 tttggaatgt agtcgtcagc gccaatctcc aggcctacga tacggtccac ttcttcccct
    65221 ttcgccgtca ccataatgat cggcatcggg ttgctctgac tacgaagacg tcggcaaatc
    65281 gacaagccat cttcaccagg taacattaaa tccagtacca taagatggaa agattcacga
    65341 gtcagcaggc gatccatctg ttctgcatta gcgacgcttc gaacctggaa gccttgttcg
    65401 gtgagataac gttccagcag cgcacgcagg cgcatgtcgt catcgaccac cagaatcttg
    65461 tagttctctt gcattgtttg tactcccaaa ggttcgcaac aatttgtaag cgtgtattct
    65521 taaaaaagct cacgttcgtc accagctaaa tctggtatga atttcagcct aaattgttac
    65581 aaagcatatt aaacagcagc ttaagtatac aatttattcg gcgaaacatt attgattctg
    65641 ttgatatgat cacgttatac ccaatgtgcg cattatcaaa cagacaaagg gaatcaacga
    65701 gatgaaaacg cccctggtta cccgggaagg gtatgaaaaa ctcaaacaag agcttaatta
    65761 tctctggcgt gaagaacgcc cggaggtcac aaaaaaggtg acctgggccg caagtctggg
    65821 cgaccgcagc gaaaatgctg actatcagta taataaaaag cgtctgcgtg aaatcgaccg
    65881 tcgcgtgcgc tatctcacta aatgcctgga aaatctcaaa attgtcgatt actcccctca
    65941 gcaggaaggc aaagtctttt ttggcgcgtg ggtggagatt gaaaacgacg atggcgtgac
    66001 tcaccgtttc cgtattgtcg gctacgatga aatttttggc cgtaaagatt acatctctat
    66061 cgattccccg atggcccgcg cattgctgaa aaaagaagtc ggcgatctgg cggtggtgaa
    66121 tacccctgcc ggggaagcga actggtatgt taatgctatc gagtacgtga aaccgtaagg
    66181 aagagtctta acctcctgcc gatggctggc atttttgcca gccagtccgt ataactatcc
    66241 cctgattttt gatccgaaaa gatgaactca aaccatgatg aatgattcgt tctgccgcat
    66301 tattgcgggt gaaattcagg cgcgcccgga acaggttgac gctgccgttc gcctgcttga
    66361 tgaagggaat accgtgccgt ttatcgcacg ttatcgtaag gaaatcaccg gcggtctgga
    66421 tgacacgcag ctgcgtaatc tggaaacgcg tctgagctat ctgcgcgagc tggaagagag
    66481 acgtcaggcg atcctcaaat ccatttccga gcaaggcaaa ctcaccgatg atctggcgaa
    66541 ggccatcaac gccaccctaa gcaaaaccga actcgaagac ctctacctgc cctacaaacc
    66601 taaacgccgc acccgcgggc aaatcgccat tgaagcaggg cttgagccgt tggctgacct
    66661 gctgtggagc gatccgtcac acacgccaga agtcgccgct gcacaatatg ttgatgccga
    66721 taaaggcgtg gcagatacca aagccgcgct ggatggcgcg cgctatatcc tgatggaacg
    66781 gtttgccgaa gatgccgcgc tgctggcgaa agtgcgtgat tatctgtgga agaacgcgca
    66841 tttggtttct acggtggtga gcggtaaaga agaggaaggg gcgaaattcc gcgactattt
    66901 cgatcatcac gaaccgttgt ccacggtgcc ttctcaccgc gcgctggcga tgttccgtgg
    66961 gcgtaacgaa ggcgtactcc agctttcgct gaatgccgat ccgcagttcg atgagccgcc
    67021 caaagagagc tattgcgagc aaatcatcat ggatcacctt ggcctgcgcc tgaacaatgc
    67081 cccggcggat agctggcgca aaagcgtagt gagctggacc tggcgcatca aggtgctgat
    67141 gcatctggaa accgaactga tgggtaccgt gcgcgaacgc gcggaagatg aagcaatcaa
    67201 cgtctttgcc cgtaacctgc acgatctgct gatggcggcc cctgccggac tgcgtgcaac
    67261 gatgggcctc gatccgggtc tgcgtaccgg ggtaaaagtg gcggtggtcg atgccactgg
    67321 caaactggtg gcgaccgata ccatttaccc gcacaccgga caagccgcaa aagcagcgat
    67381 gaccgttgct gccttgtgtg aaaaacataa cgttgaactg gtggcgatcg gcaacggtac
    67441 agcttcccgc gaaactgaac gtttctatct cgacgtgcag aagcagttcc cgaaagtgac
    67501 cgcacagaaa gtgatcgtca gcgaagctgg cgcgtcggtt tactcagctt ccgagctggc
    67561 agcgcaggag ttcccggatc tcgacgtttc gctgcgtggt gcggtatcta tcgcccgccg
    67621 tttgcaggat ccgctggcgg agctggtgaa aatcgatccg aaatctatcg gcgtaggtca
    67681 gtatcagcat gacgtcagcc agacgcaact ggcccgcaaa ctggatgcag tagtagaaga
    67741 ctgcgtaaac ggcgttggcg tcgatctcaa caccgcttct gttccgctat taacccgcgt
    67801 ggcgggcctg acgcgcatga tggcgcaaaa catcgtggcc tggcgcgatg agaacggcca
    67861 gttccagaac cgtcagcaac tgctgaaagt cagccgtctg gggccgaaag ccttcgagca
    67921 gtgcgcgggc ttcctgcgca ttaaccacgg tgataacccg ctggacgcct ctaccgttca
    67981 tccggaagcc tatccggtgg tggaacgcat tctggcagca acacagcagg cactgaaaga
    68041 tctgatgggt aacagcagcg aactgcgtaa cctgaaagcg tctgacttta ctgatgaaaa
    68101 attcggtgtg ccgacagtaa ctgacatcat caaagagctg gaaaaaccgg gtcgcgatcc
    68161 gcgtccggaa tttaaaaccg ctcagtttgc cgatggcgtc gagacaatga acgacctgca
    68221 accgggtatg atcctcgaag gcgcagtgac caacgtcacc aactttggcg cgtttgtcga
    68281 tattggcgtg catcaggacg gcctggttca catctcttca ttgtcgaaca agtttgtgga
    68341 agatccgcat accgtggtga aagcgggcga cattgtgaag gtgaaagtgc tggaagtgga
    68401 tcttcagcgt aaacgtatcg ccctgactat gcgtctggac gagcagcctg gcgaaaccaa
    68461 ctctcgtcgc ggcggcggta atgaacgccc gcaaaacaac cgcccggcag ccaaaccacg
    68521 cggtcgtgaa gcgcagcctg ccggtaatag cgcgatgatg gatgcgctgg cggcagcgat
    68581 gggtaaaaaa cgttaaacgc ccggactggc ccctaaacct gcgcgataag gcgttcacgc
    68641 cgcatctggc aacgaacgct ttgcctgaca taaaagtgcc ggagaatatc tccggcactt
    68701 ttattccaca gccaaactca caatatactc cgttaatatt tatcacttca ttaacaactg
    68761 aaaccttaat taaacattag ccagtccggg taactcacta ttcgaattat attttcgctg
    68821 cgatataacc ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgta
    68881 cacattttaa gtattgctga tagaaaccat tctcattatc attgtgttgt tgattattta
    68941 atctctcctt tgttggcaaa tcatctggtc tcatgtcgcg gtcaaacgcc ccatgaggta
    69001 gttatccagt taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg
    69061 aaaatcactg gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctcta
    69121 ggcatgttac ctggctcctc ttttgatgtg gtgcgcgtcg ctccactcgg cgaccccatt
    69181 catatcgaaa cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa
    69241 gtggaagcgg tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccattggctt
    69301 aattggtaat ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca
    69361 gcgtgtaggt aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac
    69421 cgatcatcag gtcacgctgg tggacctgcc cggcacctat tctctgacca ccatctcatc
    69481 gcagacctcg ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct
    69541 gctgattaac gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctgcaact
    69601 gctggaactc ggcattccct gcatcgtggc gctgaacatg ctcgacattg ccgagaagca
    69661 aaatattcgt attgaaattg atgctctatc ggcacgtctt ggctgcccgg tgatcccgct
    69721 ggtttcaacc cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc
    69781 taacgagaat gtggaactgg tgcattacgc acagccgcta ctcaacgaag cagattcact
    69841 ggcaaaagtg atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat
    69901 gctggaaggc gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc
    69961 caccctcgcc cgtctgcgta atgaaatgga tgatccggcg ctgcacattg ccgatgcgcg
    70021 ttaccagtgc attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag
    70081 ccgtttcacc actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt
    70141 cctctttgtg atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc
    70201 gctgtttgac gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac
    70261 gctccacttc ccggactggc tgactatctt cctcgcccag ggcatgggtg gcggtattaa
    70321 caccgtgctg ccactggtgc cgcagatcgg catgatgtac ctgttcctct ccttccttga
    70381 ggactccggc tatatggcgc gtgcagcgtt tgtgatggac cgcctgatgc aggcgctggg
    70441 cttgccgggg aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt
    70501 aatgggcgca cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc
    70561 gtttatgtcc tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg
    70621 gcagaacggt gccctggcgg ttttctcgct gtatatgctc ggtatcgtga tggcggtgct
    70681 gactggcctg atgctcaagt acaccatcat gcgcggtgaa gcaacgccgt ttgtcatgga
    70741 gctgccggta taccatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct
    70801 gaagggcttc gttctgcgtg ctggtaaagt gattatcatc gtcagcattt tcctgagcgc
    70861 tttcaacagc ttctcgttga gcgggaaaat cgtcgataac atcaacgact cggcgctggc
    70921 ttccgtcagc cgggtgataa cccctgtctt caagccgatt ggcgtacatg aagataactg
    70981 gcaggcaacg gttggcctgt ttacgggcgc aatggcaaaa gaagtggtgg tgggtacgct
    71041 caacaccctc tacaccgcag aaaatattca ggacgaagag ttcaatccgg cggaattcaa
    71101 cctcggtgaa gagctgttca gtgcggtaga tgaaacctgg cagagcctga aagacacctt
    71161 cagccttagc gtactgatga accccattga agctagcaaa ggcgacggtg aaatgggtac
    71221 cggggcgatg ggcgtgatgg atcagaaatt cggtagcgca gctgccgcat acagctacct
    71281 gattttcgtc ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc
    71341 aagccgtggc tggatgggct tctccatcct gtgggggctg aatatcgctt actcactggc
    71401 aacattgttc tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat
    71461 tctggcggta atcctgttta acatcgtagt tatcggtctg ctgcgccgcg cgcgtagccg
    71521 ggtggatatc gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac
    71581 caccggtgat tgccattaat ggcttcactt attcaggtac gtgatttgct ggcgttacgg
    71641 ggccgtatgg aagcgaccca gataagccag acattgaaca ctccgcagcc aatgattaac
    71701 gccatgctgc aacagctgga aagcatgggc aaagccgtgc ggattcagga agaacctgac
    71761 ggctgcctct ctggcagttg taaaagctgc ccggaaggca aagcctgtct gcgcgagtgg
    71821 tgggcgctgc gttaaccata ctccatcgcc tgttttgaaa agcccggtat gcgtttgcat
    71881 ccgggctttt ttgcgtgcgg ctttccataa aaatgcaatt cttgcagcgc aacgtaagtt
    71941 cctctgaaag cgtctcgcag ggatgaaaac tcgctaatgc acagatgtgg agtggcgcgt
    72001 agagtcgcgg cattcaaaca acaggtgaag gaacgccatg agtaaaaagc agagttccac
    72061 cccacatgat gcgctgttca aactcttttt acgccaaccg gagacggcac gcgattttct
    72121 tgcgtttcat ttaccggcac ccattcacgc gctctgtgat atgaaaaccc tcaagctgga
    72181 gtcgagcagc tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt
    72241 gaaaacggaa caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa
    72301 caaactgatc gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga
    72361 tgctggatac aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag
    72421 cccctatccc tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca
    72481 gctttatgcc tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat
    72541 gcagcaccga cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgatct
    72601 gatggggctg gtagagcaaa tggcctgctt attaagtagt ggatacgcta atgacagaca
    72661 aatcaaaggg ctgtttaatt acatactgca aactggcgac gctgtacgtt ttaacgattt
    72721 tatcgacggc gttgccgaac gttcaccgaa acacaaggag agtttaatga ctattgcgga
    72781 aagattgcgg caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga
    72841 atccggagtc cctcttgcag acatcatgcg atttaccggg ctgtcagaag aagagttggc
    72901 tgcggcgagt cagtagatac tctaccctgc ttaagtgcta ctttgtgcta tcttgcatgt
    72961 aacacaaagt agcacttagg gaactgatat gcagacagtg aaattacggc aacaaggagg
    73021 agccatgatc gttactatcc cacgagatct tgccatcgat cttggctggt caccgggcac
    73081 agagcttacc gtggagaaaa aaggcgatag cgttaatttg cgcgccacag agcacaaacc
    73141 gcgtgggcgt cttaccgtcg cgcagttgtt gagccaaatc gatgaaagcg aaattacgga
    73201 gctaaatcag tccacagaag gatgggctga agggaaaaaa ggtaatgaag catggtaaag
    73261 gcacggacgc cacatcgtgg tgagatctgg tattttaacc ctgatccggt tgccgggcat
    73321 gaacttcagg ggccacatta ttgcattgtg gtaacggaca aaaaactcaa caatgtttta
    73381 aaagttgcta tgtgctgccc gatttcaaca ggggcaaatg cagcacgttc cacaggggtg
    73441 acggtgaacg tcctcccctg tgatacgcaa accggtaacc tgcatggcgt tgtactttgt
    73501 caccagctaa aagccgtcga tcttattgcc cgtggcgcta aatttcatac cgttgccgat
    73561 gaaaaattga ttagtgaagt tatcagtaaa ctggtgaatt taatcgaccc acaataatta
    73621 gctctcactt accaccaaca ccctctgctt caacgccacc agcaggtgac aaaactcgac
    73681 cggatgcgaa ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg
    73741 ccaaagttta tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata
    73801 caatcgcaaa aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag
    73861 gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt
    73921 cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa
    73981 ccgctccact gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc
    74041 cggttttatc cccggccact cgtcacgagc actaaaataa ggtgacgacg ccacggtgac
    74101 cagcgcctga acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag
    74161 actccagcct aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc
    74221 agcaagtgac agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg
    74281 cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc
    74341 ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt
    74401 attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg
    74461 attatgctgg ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg
    74521 ttcacgcgcc acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca
    74581 ctcccatctt ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac
    74641 ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg
    74701 cagtgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg
    74761 caccaccggg ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca
    74821 ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca
    74881 ctgccaatgg gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct
    74941 cagtgcccgg ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca
    75001 aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat
    75061 tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac
    75121 cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca
    75181 ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc
    75241 aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat
    75301 gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg
    75361 acctgctgac cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg
    75421 actttgttac cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc
    75481 gtaaagtggc agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga
    75541 tcaacccaca gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg
    75601 gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc
    75661 tgaaagaagg gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg
    75721 atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag
    75781 attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt
    75841 ggcacagcat tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct
    75901 ctcataattt aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca
    75961 ttcacatggg taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta
    76021 tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga
    76081 aaattgacat tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg
    76141 ctatcggtgt aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg
    76201 ctctcgtcct cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta
    76261 ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt
    76321 ttggcgcaat gctggcaaac tgcggtggcg cacaacgtat cgccaccacg ctgattgcca
    76381 aatttggtaa aaagcacatc cagtgggcgg tggtgttaac cggttttacc gttggttttg
    76441 ccctgttcta tgaagtgggc ttcgtgctga tgctgccgct ggtgtttacc atcgcggctt
    76501 ccgcgaatat tccattgctg tatgttggtg taccaatggc ggctgcactg tctgtgaccc
    76561 acggcttcct gccaccgcat ccgggcccga ctgcgattgc caccattttc aatgccgata
    76621 tgggtaaaac cctgctgtac ggtactattc tggcaatccc gaccgtgatt ctcgccggtc
    76681 cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat cccggaaggt ctctacagcg
    76741 cgaaaacctt cagcgaagaa gagatgccga gctttggcgt cagcgtctgg acctctctgg
    76801 tgccggttgt actgatggcg atgcgtgcga ttgccgaaat gatcctgccg aaaggtcacg
    76861 ctttcctgcc ggtagcggag ttcctcggtg acccggtaat ggcaacgctg attgccgtgc
    76921 tgattgcgat gttcaccttt ggtctgaacc gtggtcgttc aatggatcag attaacgaca
    76981 cgctggtttc ttccatcaaa atcattgcga tgatgctgtt gatcatcggt ggtggcggtg
    77041 cgttcaagca ggtgctggta gacagcggcg tggacaaata cattgcttcc atgatgcacg
    77101 aaaccaacat ttctccgctg ctgatggcct ggtcgattgc tgccgtactg cgtatcgcgc
    77161 tgggttccgc aaccgttgcg gcaatcactg cgggtggtat cgcggcaccg ctgattgcaa
    77221 cgacgggtgt tagcccagag ctgatggtta ttgcggtggg ttccggtagt gtgattttct
    77281 ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta ctttaacctg actatcggcg
    77341 agaccatcaa atcctggtcg atgctggaaa ccattatctc ggtgtgcggt ctggtaggct
    77401 gtctgctgct gaatatggtg atttgataac actgactgcc ggatgcatcg catccgacat
    77461 caaccgcact ctacttcttc ttcgctgcag ctctgcgccg tctgtccaaa tccttcagca
    77521 acttgttcac gccatcatcg gcaaacatcg actcaagcgt tgcggaaagc ttgcgtcgcc
    77581 agtttttata ctggtaactg gtgccaggaa tattcaccgg ttcggccata tccagccagt
    77641 cttccggctg tagtcctaac agagcactgt tactgtcggc aatgtagcgc tgcaaaccac
    77701 ggttcagcgt cgtcgtcatc gacatcaacg atgccttatg cccggcacgt tttggcagac
    77761 aaccatattt atgcagtgca tccagcagcc cttgcttcgc cagttcgcga tcctgataca
    77821 gaccgcgcag taccacttca tccggataca gccccagggt tttgcccagc gttagatccc
    77881 cgctctccca gtaaccacgc agcgttggca ggtcatgtgt cgccgcaacc gccatcgact
    77941 gctccggata cgctttcggt gcacggaacg tcttctcgtg gtcgttttcg aaatagagca
    78001 ctttgtaaga gtacacaccg ctgctgcgca gcttaccgac aatctctacc ggtacggtac
    78061 cgagatcttc accaatcacc atacagcgat gacgtttact ttcgagtgcc agaatcgaga
    78121 gcagatcatc caccggatag tgaacatacg cgccctgatc tgccgtctcg ccatacggta
    78181 tccaccacaa acgcagcatc gacatcacat ggtcaattcg taatgcgccg cagttttgca
    78241 tattggcacg caacagctcg ataaacggtt cataggcacg cgcggtgatg atatgcgggt
    78301 ccattggcgg taatccccag ttctgcccca acgggccgag gatatccggc ggcgcgccaa
    78361 ccgatgcttt caggcaatat agttcacggt cacaccaggt ttccgcccca ccttccgcta
    78421 cgccaaccgc cagatcacga tacaagccaa tcggcatttc atagccctgg cttatctccc
    78481 agcaggcggc aaactggctg taagccagcc actgcaacca gagataaaaa tcgacgtcat
    78541 cacgatgttc ttcgcagaac tgacgcactt ctggtgaatc cacgttctta tacatctctg
    78601 gccatgcagg ccagccccag cgcatttcgt cctctttcac ttgctgggca tgtagcgcat
    78661 caaaggctgc ctgccagaac aggctgtcgc cctgctctgc aacaaactgg cgaaacgcgg
    78721 ccatctgctc atcatcacgt tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt
    78781 ttagggcggt aaccgtggag taatcgaccc agtcggcatc gcgcgcctgt tgcagcgtct
    78841 gttgcgtggt cggcaactgc caccaggcct gagcctcttc gctaagatgg aaatcttcaa
    78901 cggcgttaac gtcgatataa atcacattca gccaacggcg agaagacggg ctgtatgggc
    78961 tggcgctctc cggatttgcc ggatagagcg catgaatcgg gttcaggcca atgaacgacc
    79021 cgccacgttt tgccacatcc accagcatcg ctttgagatc gccaaaatcc ccaatacccc
    79081 agtttttttc cgatcgcagc gtataaagct gaacgcaggc accccacagc ttttgtttat
    79141 tcagcaaagc ctgcggttcg taacaacgtt tcggggcgac aatcacccgg caatgcgcgc
    79201 gctggtcgtc ctgggtgagt gttagcgtgt gataaccttc cggcagcttc gtcggtagat
    79261 tgaacgcttt gccccccgtt acatggcctt tgtactgcgt tccttcttcg gtggtcagct
    79321 gccagctata ttcgccgctg ccctccacca ccatcggcat ttttttgccg ctggtataaa
    79381 ccatgacatt cgggactggc gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt
    79441 caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt
    79501 aattggggct aatccccgcc gccagcgcgg cattatccag acgtttgctt tccatcgagc
    79561 ttccttagcg ttttgcctgc cagatacgag cctgataatc gcgaatagag cgatccgagc
    79621 taaacatacc gcagcgggcg gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc
    79681 ggtacagcac atccacctgc ttttgtgcct caacataggc tgcgaaatcc gccatcacca
    79741 gatacggatc gccgccctgt ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat
    79801 cgccgtcgct gtatttaccg ctttccagct ctttcaatac tgcgtccagc accttatctt
    79861 tcttccgcca tttcaccggg tcgtagcctt tggccagaat agccttcact tgttccacgg
    79921 tatgaccaaa aataaagata ttttcttcac cgactttctc ggcgatttca acgttcgcac
    79981 catccagcgt accgacagta agcgcaccat tgagcgccag tttcatattg ccggtaccgg
    80041 aagcttcttt acctgcagtc gaaatttgtt cggagatatc cgccgccggg atcagttttt
    80101 ccgccgccga aacgcaataa tccggcagga acaccacctt caacttatcg ccaaccagtg
    80161 gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa gataatattc ttcgccaggt
    80221 agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg cggtacgcga tcagcctgca
    80281 ggttttcacg aatttctttg tacaacgcca gaatatgcag cagattcagg tgctggcgtt
    80341 tgtactcatg cagacgtttg atctgaatat cgaaaatcgc ctgtggattg atctcaatac
    80401 cggtacgaac tttcacaaac tccgccagac ggactttatt cgcctgcttg atctcgcgat
    80461 attgctgacg gaatttcgca tcatcagcga atttttccag attgatcagc tgatcgagat
    80521 cgttagccca ctctttttgc agtgatttat ccaacagagc cgccagtgca gggttgcact
    80581 gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt atggaatttg ttcggccata
    80641 gctggtgata ttccgggaac agatctttca ccaccagatc cgagtgcagc gccgcaacac
    80701 cgttcaccgc gaaaccgcca accacacaca ggttcgccat atgcacttgt ttatcgtgca
    80761 ccaccgccag tttggcccac actttttcat cgcccggcca ggttttctcc accagcgttt
    80821 taaagcgagt attaatttcg ttaataatct gcatatggcg cggcagtaag cctttcacca
    80881 gtttcacatc ccagcgttcc agcgcttctg gcatcagggt atggttggtg taagcgaaag
    80941 ttttgctggt gatggcccag gcgtcatccc agctcatctg gtgctcatcg gtcagcacgc
    81001 gcagcagttc tggaatcgcg atagttggat gggtatcgtt cagctgaata acttcgtaat
    81061 ccgccagttc gtgcagttta cgccccgcca gatgatggcg acgcaaaata tccgctaccg
    81121 aacaggcaca ctggaagtat tgctgcatca ggcgcagctt tttaccggca gtatggttgt
    81181 cgtttggata gagaactttg gtcagttttt ccgcattgat gccttgctgt tcggcgcgca
    81241 ggaaatcacc gtcgttaaat ttagttagat caaacggatg cgcatgcgtc gcctgccaca
    81301 gacgcagcgg ctgcgccacg ccattacgat agccgacaac ggggagatcc cacgcttgac
    81361 cggtaatggt aaactccggc tcccagcgtc cgtctttcat cactttaccg ccaatcccta
    81421 cctgcacatc cagtgcttcg ttgtggcgga accacgggta gttactgcga tgccagtcat
    81481 ccggcgcttc aacctgtttg ccatcgacaa aagactggcg gaacaaacca tattgatagt
    81541 tcagaccgta acccgtcgca gactgaccga cagttgccat tgagtcgagg aagcacgccg
    81601 ccagacgtcc cagaccaccg ttacccagcg ccgggtcgat ctcttcttcc agcaggtcag
    81661 tcaggttgat gtcataagcc ttcaacgaat cctgtacatc ctgataccag ccgagattca
    81721 acaggttgtt gcccgtcaga cgaccaatca aaaactccat tgagatgtag ttaacatgtc
    81781 gctgattcgc caccggtttg gcgaatggct gagcacgcag catttcggcc agtgcttcac
    81841 tcactgccag ccaccactgg cgaggagtca tttcagccgc agaatttaag ccataacgct
    81901 gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag
    81961 gagttccact tttcttagat tttcaacaca acgttatcgc tagtttgcca ggctcgatgt
    82021 tgaccttcct catcctgcgg gggattaggc agggaggagt tgcggggatg agcaaggaaa
    82081 tgtgatctcg accacttaaa gctagtgcaa accacaggat tagcagcaaa tcaatgccat
    82141 acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg
    82201 gaaggtaact ctataaatta agtaaaggag tgaaacagtt tcacaagtaa aatatccagt
    82261 gtgctccatc tcattcttaa tagatttatt aagatcatct ttttagatgg cactttcatc
    82321 aggaatgaag aagaaaccct tgcttaaatg aatctgat
//
LOCUS       DADLTI010000003        78957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLTI010000003 DADLTI010000000
VERSION     DADLTI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.003"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1592)
                     /locus_tag="JN755_000180"
     CDS             complement(<1..1592)
                     /locus_tag="JN755_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7379106.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JN755_000181"
     CDS             complement(1576..1932)
                     /locus_tag="JN755_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7379107.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JN755_000182"
     CDS             complement(2055..2237)
                     /locus_tag="JN755_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379108.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JN755_000183"
     CDS             complement(2221..2661)
                     /locus_tag="JN755_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7379109.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JN755_000184"
     CDS             complement(2661..2957)
                     /locus_tag="JN755_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7379110.1"
                     /translation="MTEELITLEDVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JN755_000185"
     CDS             complement(2954..3292)
                     /locus_tag="JN755_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7379111.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JN755_000186"
     CDS             complement(3289..4500)
                     /locus_tag="JN755_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7379112.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JN755_000187"
     CDS             complement(4502..5074)
                     /locus_tag="JN755_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7379113.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JN755_000188"
     CDS             complement(5114..6271)
                     /locus_tag="JN755_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7379114.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6571..6873)
                     /locus_tag="JN755_000189"
     CDS             complement(6571..6873)
                     /locus_tag="JN755_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379115.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6937..7098)
                     /locus_tag="JN755_000190"
     CDS             complement(6937..7098)
                     /locus_tag="JN755_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7379116.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7108..7413)
                     /locus_tag="JN755_000191"
     CDS             complement(7108..7413)
                     /locus_tag="JN755_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379117.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7410..7688)
                     /locus_tag="JN755_000192"
     CDS             complement(7410..7688)
                     /locus_tag="JN755_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379118.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7968..9716)
                     /locus_tag="JN755_000193"
     CDS             complement(7968..9716)
                     /locus_tag="JN755_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7379119.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9713..10012)
                     /locus_tag="JN755_000194"
     CDS             complement(9713..10012)
                     /locus_tag="JN755_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7379120.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10018..10251)
                     /locus_tag="JN755_000195"
     CDS             complement(10018..10251)
                     /locus_tag="JN755_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379121.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10253..10473)
                     /locus_tag="JN755_000196"
                     /pseudo
     CDS             complement(10253..10473)
                     /locus_tag="JN755_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10466..10687)
                     /locus_tag="JN755_000197"
     CDS             complement(10466..10687)
                     /locus_tag="JN755_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379122.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10677..10877)
                     /locus_tag="JN755_000198"
     CDS             complement(10677..10877)
                     /locus_tag="JN755_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY7379123.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10886..11632)
                     /locus_tag="JN755_000199"
     CDS             complement(10886..11632)
                     /locus_tag="JN755_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379124.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11690..11878)
                     /locus_tag="JN755_000200"
     CDS             complement(11690..11878)
                     /locus_tag="JN755_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7379125.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12283..13512)
                     /locus_tag="JN755_000201"
     CDS             complement(12283..13512)
                     /locus_tag="JN755_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7379126.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13761..14882
                     /locus_tag="JN755_000202"
     CDS             13761..14882
                     /locus_tag="JN755_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7379127.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14931..16157)
                     /gene="pepT"
                     /locus_tag="JN755_000203"
     CDS             complement(14931..16157)
                     /gene="pepT"
                     /locus_tag="JN755_000203"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7379128.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16407..17543
                     /gene="potA"
                     /locus_tag="JN755_000204"
     CDS             16407..17543
                     /gene="potA"
                     /locus_tag="JN755_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7379129.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17527..18384
                     /gene="potB"
                     /locus_tag="JN755_000205"
     CDS             17527..18384
                     /gene="potB"
                     /locus_tag="JN755_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7379130.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18381..19175
                     /gene="potC"
                     /locus_tag="JN755_000206"
     CDS             18381..19175
                     /gene="potC"
                     /locus_tag="JN755_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7379131.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19172..20218
                     /gene="potD"
                     /locus_tag="JN755_000207"
     CDS             19172..20218
                     /gene="potD"
                     /locus_tag="JN755_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7379132.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20374..21195)
                     /gene="cobB"
                     /locus_tag="JN755_000208"
     CDS             complement(20374..21195)
                     /gene="cobB"
                     /locus_tag="JN755_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7379133.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21211..22122)
                     /gene="nagK"
                     /locus_tag="JN755_000209"
     CDS             complement(21211..22122)
                     /gene="nagK"
                     /locus_tag="JN755_000209"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7379134.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22151..23395)
                     /gene="lolE"
                     /locus_tag="JN755_000210"
     CDS             complement(22151..23395)
                     /gene="lolE"
                     /locus_tag="JN755_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7379135.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23395..24096)
                     /gene="lolD"
                     /locus_tag="JN755_000211"
     CDS             complement(23395..24096)
                     /gene="lolD"
                     /locus_tag="JN755_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7379136.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24089..25288)
                     /gene="lolC"
                     /locus_tag="JN755_000212"
     CDS             complement(24089..25288)
                     /gene="lolC"
                     /locus_tag="JN755_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7379137.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25550..26596
                     /locus_tag="JN755_000213"
     CDS             25550..26596
                     /locus_tag="JN755_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7379138.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPPFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26724..30170
                     /gene="mfd"
                     /locus_tag="JN755_000214"
     CDS             26724..30170
                     /gene="mfd"
                     /locus_tag="JN755_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7379139.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30313..31275
                     /gene="ldtC"
                     /locus_tag="JN755_000215"
                     /pseudo
     CDS             30313..31275
                     /gene="ldtC"
                     /locus_tag="JN755_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31358..31615)
                     /gene="bhsA"
                     /locus_tag="JN755_000216"
     CDS             complement(31358..31615)
                     /gene="bhsA"
                     /locus_tag="JN755_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7379140.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31856..32488
                     /gene="comR"
                     /locus_tag="JN755_000217"
     CDS             31856..32488
                     /gene="comR"
                     /locus_tag="JN755_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7379141.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32550..33089)
                     /locus_tag="JN755_000218"
     CDS             complement(32550..33089)
                     /locus_tag="JN755_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7379142.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33316..34620)
                     /locus_tag="JN755_000219"
     CDS             complement(33316..34620)
                     /locus_tag="JN755_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7379143.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35021..35563)
                     /locus_tag="JN755_000220"
     CDS             complement(35021..35563)
                     /locus_tag="JN755_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7379144.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35586..36611)
                     /gene="nagZ"
                     /locus_tag="JN755_000221"
     CDS             complement(35586..36611)
                     /gene="nagZ"
                     /locus_tag="JN755_000221"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7379145.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36622..37446)
                     /gene="thiK"
                     /locus_tag="JN755_000222"
     CDS             complement(36622..37446)
                     /gene="thiK"
                     /locus_tag="JN755_000222"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7379146.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37427..38068)
                     /gene="lpoB"
                     /locus_tag="JN755_000223"
     CDS             complement(37427..38068)
                     /gene="lpoB"
                     /locus_tag="JN755_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7379147.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38083..38460)
                     /locus_tag="JN755_000224"
     CDS             complement(38083..38460)
                     /locus_tag="JN755_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7379148.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38463..38822)
                     /gene="hinT"
                     /locus_tag="JN755_000225"
     CDS             complement(38463..38822)
                     /gene="hinT"
                     /locus_tag="JN755_000225"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7379149.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39156..41345
                     /gene="fhuE"
                     /locus_tag="JN755_000226"
                     /pseudo
     CDS             39156..41345
                     /gene="fhuE"
                     /locus_tag="JN755_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41405..42838)
                     /gene="ptsG"
                     /locus_tag="JN755_000227"
     CDS             complement(41405..42838)
                     /gene="ptsG"
                     /locus_tag="JN755_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7379150.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43133..43930)
                     /locus_tag="JN755_000228"
     CDS             complement(43133..43930)
                     /locus_tag="JN755_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7379151.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43941..44945)
                     /gene="holB"
                     /locus_tag="JN755_000229"
     CDS             complement(43941..44945)
                     /gene="holB"
                     /locus_tag="JN755_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7379152.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44942..45583)
                     /locus_tag="JN755_000230"
     CDS             complement(44942..45583)
                     /locus_tag="JN755_000230"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7379153.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45573..46595)
                     /gene="yceG"
                     /locus_tag="JN755_000231"
     CDS             complement(45573..46595)
                     /gene="yceG"
                     /locus_tag="JN755_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7379154.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46598..47407)
                     /gene="pabC"
                     /locus_tag="JN755_000232"
     CDS             complement(46598..47407)
                     /gene="pabC"
                     /locus_tag="JN755_000232"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7379155.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47527..48768)
                     /gene="fabF"
                     /locus_tag="JN755_000233"
     CDS             complement(47527..48768)
                     /gene="fabF"
                     /locus_tag="JN755_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7379156.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48855..49091)
                     /gene="acpP"
                     /locus_tag="JN755_000234"
     CDS             complement(48855..49091)
                     /gene="acpP"
                     /locus_tag="JN755_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7379157.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49302..50036)
                     /gene="fabG"
                     /locus_tag="JN755_000235"
     CDS             complement(49302..50036)
                     /gene="fabG"
                     /locus_tag="JN755_000235"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7379158.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50049..50978)
                     /gene="fabD"
                     /locus_tag="JN755_000236"
     CDS             complement(50049..50978)
                     /gene="fabD"
                     /locus_tag="JN755_000236"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7379159.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50994..51947)
                     /locus_tag="JN755_000237"
     CDS             complement(50994..51947)
                     /locus_tag="JN755_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7379160.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(52015..53085)
                     /gene="plsX"
                     /locus_tag="JN755_000238"
     CDS             complement(52015..53085)
                     /gene="plsX"
                     /locus_tag="JN755_000238"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7379161.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53166..53339)
                     /gene="rpmF"
                     /locus_tag="JN755_000239"
     CDS             complement(53166..53339)
                     /gene="rpmF"
                     /locus_tag="JN755_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7379162.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53391..53912)
                     /gene="yceD"
                     /locus_tag="JN755_000240"
     CDS             complement(53391..53912)
                     /gene="yceD"
                     /locus_tag="JN755_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7379163.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54111..54695
                     /gene="yceF"
                     /locus_tag="JN755_000241"
     CDS             54111..54695
                     /gene="yceF"
                     /locus_tag="JN755_000241"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7379164.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54807..55766)
                     /gene="rluC"
                     /locus_tag="JN755_000242"
     CDS             complement(54807..55766)
                     /gene="rluC"
                     /locus_tag="JN755_000242"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7379165.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56339..59524
                     /gene="rne"
                     /locus_tag="JN755_000243"
     CDS             56339..59524
                     /gene="rne"
                     /locus_tag="JN755_000243"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7379166.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59721..60674)
                     /gene="flgL"
                     /locus_tag="JN755_000244"
     CDS             complement(59721..60674)
                     /gene="flgL"
                     /locus_tag="JN755_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY7379167.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60686..62328)
                     /gene="flgK"
                     /locus_tag="JN755_000245"
                     /pseudo
     CDS             complement(60686..62328)
                     /gene="flgK"
                     /locus_tag="JN755_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62394..63335)
                     /gene="flgJ"
                     /locus_tag="JN755_000246"
     CDS             complement(62394..63335)
                     /gene="flgJ"
                     /locus_tag="JN755_000246"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7379168.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63335..64432)
                     /locus_tag="JN755_000247"
     CDS             complement(63335..64432)
                     /locus_tag="JN755_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7379169.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64444..65142)
                     /gene="flgH"
                     /locus_tag="JN755_000248"
     CDS             complement(64444..65142)
                     /gene="flgH"
                     /locus_tag="JN755_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7379170.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65195..65977)
                     /gene="flgG"
                     /locus_tag="JN755_000249"
     CDS             complement(65195..65977)
                     /gene="flgG"
                     /locus_tag="JN755_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7379171.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66115..66866)
                     /locus_tag="JN755_000250"
                     /pseudo
     CDS             complement(66115..66866)
                     /locus_tag="JN755_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66886..68805)
                     /gene="flgD"
                     /locus_tag="JN755_000251"
                     /pseudo
     CDS             complement(66886..68805)
                     /gene="flgD"
                     /locus_tag="JN755_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68817..69220)
                     /gene="flgC"
                     /locus_tag="JN755_000252"
                     /pseudo
     CDS             complement(68817..69220)
                     /gene="flgC"
                     /locus_tag="JN755_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69224..69397)
                     /locus_tag="JN755_000253"
     CDS             complement(69224..69397)
                     /locus_tag="JN755_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY7379172.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69552..70211
                     /gene="flgA"
                     /locus_tag="JN755_000254"
     CDS             69552..70211
                     /gene="flgA"
                     /locus_tag="JN755_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7379173.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70287..70563
                     /gene="flgM"
                     /locus_tag="JN755_000255"
                     /pseudo
     CDS             70287..70563
                     /gene="flgM"
                     /locus_tag="JN755_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70568..70984
                     /gene="flgN"
                     /locus_tag="JN755_000256"
     CDS             70568..70984
                     /gene="flgN"
                     /locus_tag="JN755_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7379174.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71024..72559)
                     /gene="murJ"
                     /locus_tag="JN755_000257"
     CDS             complement(71024..72559)
                     /gene="murJ"
                     /locus_tag="JN755_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7379175.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72726..73592)
                     /locus_tag="JN755_000258"
     CDS             complement(72726..73592)
                     /locus_tag="JN755_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7379176.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73594..74241)
                     /locus_tag="JN755_000259"
     CDS             complement(73594..74241)
                     /locus_tag="JN755_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7379177.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74252..74836)
                     /gene="rimJ"
                     /locus_tag="JN755_000260"
     CDS             complement(74252..74836)
                     /gene="rimJ"
                     /locus_tag="JN755_000260"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7379178.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75072..76280
                     /gene="mdtH"
                     /locus_tag="JN755_000261"
     CDS             75072..76280
                     /gene="mdtH"
                     /locus_tag="JN755_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7379179.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76344..76991
                     /gene="grxB"
                     /locus_tag="JN755_000262"
     CDS             76344..76991
                     /gene="grxB"
                     /locus_tag="JN755_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7379180.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77125..77685
                     /locus_tag="JN755_000263"
     CDS             77125..77685
                     /locus_tag="JN755_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7379181.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77791..78837
                     /gene="pyrC"
                     /locus_tag="JN755_000264"
     CDS             77791..78837
                     /gene="pyrC"
                     /locus_tag="JN755_000264"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7379182.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        18801 a        21202 c        19507 g        19447 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttt gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacgtc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatag tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat
     6601 caatgcatct gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac
     6661 aaaaccatga tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt
     6721 gatattcatc gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc
     6781 aaatttcatc ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg
     6841 ccggaccgtg acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc
     6901 ggattgctcc ggcttcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc
     6961 catgtctgcg taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg
     7021 ctccggtgtg cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc
     7081 tgcaatttgt gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat
     7141 ctcttccatc attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac
     7201 gggtttcatg cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc
     7261 agggtaacta agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc
     7321 aacccaccag cctggtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt
     7381 gtaaaatttt tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg
     7441 attgttgtta cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc
     7501 gccgtcgtgg tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc
     7561 gcgtggcggt ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg
     7621 cgatatttcg gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa
     7681 gtgtgcatat ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc
     7741 taccttagtg gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat
     7801 ccactatcta ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt
     7861 agatagtggg tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg
     7921 tatttttctt tactcaggta gatagggtag acagcaatta caaaaaatta taaaaacgcg
     7981 tcgcactcgt ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata
     8041 tattcatgtc cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt
     8101 agcggtttgc tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt
     8161 ctggtcgtgt acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc
     8221 agaaaattca ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga
     8281 gcttcatcac cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag
     8341 ttcgccagca atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc
     8401 ctgtcttttt cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc
     8461 acacctccgg cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc
     8521 ctgattacag ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc
     8581 gtgattttct tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata
     8641 agacgactcc cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg
     8701 cttaccgtgt tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg
     8761 ctcccgccgt ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc
     8821 atgtacagcg cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca
     8881 agccatttat gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg
     8941 tactcaatgc cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc
     9001 gttttcaggt caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt
     9061 ggttcggcaa taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg
     9121 gccctgtgct ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga
     9181 caccataccc cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca
     9241 tcaaagcgct cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta
     9301 tttttctgct ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag
     9361 cgccgccagt tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga
     9421 atatctgccg ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg
     9481 gcctggtaca gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg
     9541 gcaagctgat ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc
     9601 tgctcctgcc attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct
     9661 gtgtctttgt gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt
     9721 tacccctgaa tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc
     9781 tgctggttgg tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat
     9841 aaacaggcat cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg
     9901 ccacgccttt aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa
     9961 atttagggtg agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta
    10021 ttctccggtg tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat
    10081 tattacaggc tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg
    10141 ttcattgtcg ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg
    10201 agaaagtgca agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc
    10261 cgtccgtcgt ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat
    10321 tcagtacatc aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct
    10381 aataagttct ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc
    10441 ctgtttcagg gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa
    10501 caagaatatt tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg
    10561 cgaactgtgt ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat
    10621 caataagcgc gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat
    10681 tgtgcatagg tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg
    10741 caacggctac gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg
    10801 gtactcagtg ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata
    10861 tgatttacgg caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg
    10921 ctcctacagt gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata
    10981 aatacattac atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc
    11041 ctccgttcca gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac
    11101 aggacggcga tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc
    11161 aacgcggcat agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc
    11221 gcattgcttt acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg
    11281 cacgaaaaag acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt
    11341 ttgcccttga gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc
    11401 aacgcactga accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt
    11461 gcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc
    11521 tccatttcgg ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt
    11581 caccagggcg aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag
    11641 ctctgtgttc agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg
    11701 ccttgcagct atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc
    11761 caagtttacg acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc
    11821 taaggcctgt tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta
    11881 ttgccccctt tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga
    11941 ttgtgtcagg atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga
    12001 ataatcagga taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat
    12061 gccttttttc tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaagacgtt
    12121 aaaaatcagt tacttataaa tctgtacact ttttcacctc ttgttcgtag ttgttccgca
    12181 ttgttgctca ttgttgctca ttgttgctca ttgttgctca ttgttgcacg ttgtatctgt
    12241 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    12301 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    12361 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    12421 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    12481 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    12541 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    12601 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    12661 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    12721 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    12781 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    12841 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gttttttccc
    12901 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    12961 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    13021 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    13081 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    13141 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    13201 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    13261 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    13321 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    13381 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    13441 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    13501 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    13561 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    13621 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    13681 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    13741 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    13801 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    13861 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    13921 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    13981 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    14041 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    14101 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    14161 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    14221 gcccacatcc agatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    14281 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    14341 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    14401 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    14461 cacctcgcgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    14521 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    14581 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    14641 acgatgcact gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    14701 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    14761 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    14821 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    14881 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    14941 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    15001 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    15061 aatcccataa acgacaactg cgcgccgttg gtaccgccgc ggatcggttt cagttccggt
    15121 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    15181 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    15241 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    15301 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    15361 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    15421 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    15481 gtgcccggat gaacattgtt accgacaatt ttgatattga ccgatgcggc gttgaagttt
    15541 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    15601 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    15661 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    15721 gcaatacctg ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcaacgtc
    15781 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    15841 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    15901 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    15961 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    16021 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    16081 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    16141 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    16201 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    16261 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    16321 tttgccgtag aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc
    16381 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    16441 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    16501 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    16561 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    16621 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    16681 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    16741 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    16801 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    16861 aacagcaacg cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg
    16921 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    16981 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    17041 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    17101 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    17161 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    17221 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    17281 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    17341 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    17401 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    17461 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    17521 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    17581 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    17641 gacgacgcca gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat
    17701 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    17761 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    17821 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    17881 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    17941 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    18001 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    18061 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    18121 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    18181 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    18241 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    18301 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    18361 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    18421 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    18481 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    18541 atagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    18601 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    18661 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    18721 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    18781 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    18841 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    18901 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    18961 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    19021 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    19081 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    19141 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    19201 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    19261 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    19321 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    19381 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    19441 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    19501 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    19561 ctggggtgcg acggcgattg gtgttaacgg tgatgcagtg gatccgaaat ctgtcaccag
    19621 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    19681 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    19741 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    19801 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    19861 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    19921 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    19981 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    20041 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    20101 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    20161 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    20221 caacaagcgg acggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    20281 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    20341 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    20401 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    20461 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    20521 aaacccagca gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    20581 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    20641 cggacgcaag ggtgccggga agtggcaaca atggcattta tcttctggcg taacgtctcc
    20701 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    20761 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    20821 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    20881 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    20941 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    21001 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    21061 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    21121 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    21181 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    21241 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg
    21301 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    21361 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    21421 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    21481 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    21541 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    21601 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    21661 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    21721 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcctccca
    21781 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    21841 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    21901 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    21961 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    22021 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    22081 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    22141 tgtactgcct ttactgaccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    22201 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    22261 ccagccagtg caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact
    22321 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    22381 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    22441 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    22501 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    22561 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    22621 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    22681 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    22741 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    22801 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    22861 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    22921 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    22981 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    23041 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    23101 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    23161 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    23221 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    23281 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    23341 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    23401 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    23461 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    23521 attcaactct ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    23581 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    23641 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    23701 cgcttttaac atctccagtg cacggctgtt gatttcagcg ggctttttct tgccaatcag
    23761 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    23821 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    23881 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    23941 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    24001 cacgctgaaa ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    24061 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    24121 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    24181 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    24241 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    24301 ctccgaggat tgcaccgata atcccggcgc tggccccttg caccataaag accatcatga
    24361 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    24421 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    24481 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    24541 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactttca
    24601 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    24661 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    24721 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    24781 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    24841 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    24901 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    24961 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    25021 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    25081 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    25141 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    25201 aaagccagga gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaagc
    25261 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    25321 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    25381 tctgaaaagc ccccgtaaat caatcggcta aattgccatg ttttatcatc aatcttgccg
    25441 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    25501 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    25561 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    25621 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    25681 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    25741 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    25801 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    25861 acctgagcgc gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt
    25921 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    25981 tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca ccattcgcct tgtttgtact
    26041 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg
    26101 caatttgcct tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa
    26161 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg
    26221 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    26281 gctgttttat cagtacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat
    26341 tggctccaac accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt
    26401 aactctgctt gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct
    26461 gctggtttat ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    26521 actttcacag cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    26581 cagttactct cgttaaacca tcaccccagc cggggcgatt caatttgcta aaccatgtcg
    26641 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    26701 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    26761 cgggtgagca gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg
    26821 aaattgccga acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc
    26881 tgcgtttgca tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact
    26941 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    27001 ccacccttta ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc
    27061 ttatgcagcg cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag
    27121 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgctatg
    27181 ttgaccaggt gatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc
    27241 cgatggggag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc
    27301 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc
    27361 ccgcacacga atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    27421 ataccttcga agtgaagcgc gatcctgaac atatttacca gcaagtgagt aaaggcacat
    27481 tacctgccgg gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt
    27541 tcagttattt ccctgccaat accttgctcg tgaatactgg cgatctggaa accagtgccg
    27601 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc
    27661 cgctgttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    27721 actggccccg agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt
    27781 taggtttcca gaaactgcca gacctggccg ttcaggcgca acaaaaagcg ccgctggatg
    27841 cgctgcgtaa gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag
    27901 gtcgccgtga agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta
    27961 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    28021 gttttgtcga taaggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac
    28081 gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta
    28141 accttgcgga gctgcatatt ggtcagccgg tggtccatct ggagcacggc gtcggtcgct
    28201 atgccggaat gaccacgctg gaagctggcg gcattactgg cgagtacctg atgctcacct
    28261 atgccaacga cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg
    28321 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg
    28381 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg
    28441 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt
    28501 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac
    28561 ttaacgacat gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    28621 gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtag
    28681 cggtgttggt gcctaccacc cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt
    28741 tcgccaactg gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga
    28801 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    28861 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    28921 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga
    28981 cgcttactgc aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc
    29041 tgtcgattat cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt
    29101 atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    29161 attatctcta caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg
    29221 tgcctgaagc acggattgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg
    29281 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    29341 ccgggatcga catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc
    29401 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc acatcatcag gcatatgcat
    29461 ggctgctgac gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    29521 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    29581 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    29641 cgctgtatat ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt
    29701 cgctggaaga tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc
    29761 cagatgattt catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg
    29821 ccaaaacgga aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc
    29881 tgccagatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    29941 tggggatcag gaagctggaa ggtaatgaga aaggcgggat gatcgaattt gccgagaaga
    30001 atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc
    30061 ttgatggtcc gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg
    30121 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg
    30181 gggcgtttct gcgcccccat ctgcaacatt tacaaattct ttgcactttc ctgcactatc
    30241 cgacactgtc accatccata attcaggctt atctgtttat tacaataacc ttatatttat
    30301 tatggatttt tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc
    30361 gccgctgccg tggcgctggc gctaccggca aaagccaaca cctggccgct gccgccagcg
    30421 agcagtcgtc tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg
    30481 gaagccatcg ccaaaaaata taacgtcggc tttctcgctc tgttacaggc taaccccggc
    30541 gttgatcctt acgtaccgcg cgcgggcagc gtgttaacga tcccgttgcc aaccctactt
    30601 ccagacgcgc cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac
    30661 ccgccgggta aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac
    30721 acgctgacac cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg
    30781 ccaacggcaa acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg
    30841 ccggctggac cggataaccc aatgggccat catgcgattc gtctggcggc ctatggcggc
    30901 gtttatttgc ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc
    30961 tgtattcgtc tgcgggatga cgatatcaaa acactcttta gctaggtcac cccaggcacc
    31021 aaagtgaata tcatcaacac tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg
    31081 gttgaagtac atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt
    31141 acgctgaata gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg
    31201 caacatgtga tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc
    31261 ccacaaactc tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa
    31321 aaaatcagca gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc
    31381 catggagggt attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat
    31441 ccgctttttg cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga
    31501 tagtaccgac tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg
    31561 caaatgacat ggagcttaaa atcgccgcag cgatgagggt ttttacgttt ttcataatag
    31621 tggccttatg cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatgat
    31681 agatgtgatc tggatcacat acaagatatt ttttataaca atcattaatt aatttaaaaa
    31741 tctttaaatt tcagtcaaat agaaagcaat tatttttatg ggatctggac tggtgaacgg
    31801 aggtgattat ttttataata acctttcatt cacagtcacc aggtacaacg gcatcatggc
    31861 aactgactca acacaatgtg taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga
    31921 tgccgcgctt gataaggcca tgaaattgtt ctggcaacac ggttatgaag cgacttctct
    31981 tgcggacctc gtcgaagcga ccggagccaa agcgcccacg ctatacgcgg aatttaccaa
    32041 caaagagggg ttatttcgtg ccgttctcga ccgctatatc gatcgttttg ccgctaagca
    32101 tgaagcacag ctgttttgtg aagagaaaag cgtggagtct gcgctggctg actattttgc
    32161 tgccatcgcc aactgcttta ccagcaaaga caccccggca ggctgcttca tgatcaacaa
    32221 ctgcaccacc ctctccccag attcaggaga tatcgccaat acgttgaaat cacgccatgc
    32281 gatgcaagag cgcactttgc agcagttttt atgtcaacga caagcgcgcg gggaaatccc
    32341 gacccactgt gacgtgacac atctggcaga attccttaat tgtattattc aggggatgtc
    32401 gatcagcgca cgcgaaggtg catcgctgga aaaactgatg cagattgccg gaacgacttt
    32461 gcgtttatgg cccgaactgg tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat
    32521 gaggggccaa attgcagagt acagccttgt tatactttgt tattcaaaat cagttgcccg
    32581 ttactatcta gcgggatctg cgtacccgga tcgcggtcca tgcggatttt gccctgctga
    32641 tcgccaatct tataggtcac atcataaccg agcatttttt ctgacttgtc atacaccgtt
    32701 ttacaacgct gttgcgtagt cgtgtaagta tcgctttcct ggagagagcc ctggatctgg
    32761 ttaccggcat atccaccacc cagcgccccc acaacagtgg cgacatcttt accgcgacca
    32821 ccaccaaact gatgccctat cacgccacca gcaacagcgc cgagcaccga cccggtaatg
    32881 cgattttcat cctgcaccgg tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc
    32941 ggtgttttaa ccgtttcctt gattggggtt gcagaaacaa cctgagcgta ttgcgggccc
    33001 cgttcaaaca cgttcagact ggccactgcc gctacgccca acgcagctgc gacaccaatc
    33061 ccgatacccg ccaacattga tttattcacg ggaacacctc cttctttgca gttatgccaa
    33121 tcatgcaagc gaaggagaaa agcagctatc agacagaaga tgaaaagcgc gggaatgact
    33181 ggaaatactt aaggattcgg aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc
    33241 ttatcaggcc taccgctcgt accatttgtc cagggcggat aaggcgttta tgccacatca
    33301 gccagtgtac gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc
    33361 caccagcatc attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta
    33421 tagcgagata tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag
    33481 gttacccatc aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc
    33541 tttatactga taatttttca gcggcttacc gttcatctgc gccagaatgt tgttcattgc
    33601 gcaagtcgcc atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg
    33661 cgggcatgac gcgcagtcgc caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt
    33721 cggttccacc accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa
    33781 gtctggcgct ttgatcccgg ctgcccatac catcagatca gcctcaatat attcgccatc
    33841 tttagtgtgc aggccgcctt catcagcact ggtgaccatg gtttgcgtca gcacgcgaac
    33901 gccaagtttc gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa
    33961 aatacgttct cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta
    34021 accgtagctg tgcagttgct tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt
    34081 cgcgccgccg ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcagagta
    34141 tttcaggaac agattcagca tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag
    34201 gaaaatgcag ttctctttga cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc
    34261 catgaccagg gtgtcatagg cgattttacg ttccggaacc aacagttcac ctttctcgtc
    34321 gcgtagttct gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccaa
    34381 ctggaactgg aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc
    34441 atcaagcgag ccagtcgcca cttcgtgcag cagtggtttc cacaggtggc tgtggttacg
    34501 atcgaccagc gtaattttgg cttttttctt acgtcccagc ttatgcccca gctgtgttgc
    34561 catttccagc ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa
    34621 cgtgaccccc ttaaatttat taaccaattg ttaattaatg gttattaaca tagctcttaa
    34681 ttaacaacag gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc
    34741 attggtcata ccaaaattga tgttaatcaa gttttgttgc ttaaaagata ggcaacttta
    34801 ccgggggaga tcaaaaaata gcgtttatta tttgagcaaa ttcggaagag taatataggg
    34861 tgaagcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
    34921 ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg
    34981 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
    35041 ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
    35101 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat
    35161 cattacgcga caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac
    35221 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
    35281 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
    35341 ccgcccagta tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt
    35401 gcaacatttt gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac
    35461 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
    35521 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
    35581 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
    35641 ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
    35701 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
    35761 cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg
    35821 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc
    35881 acgccgtcaa aacccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc
    35941 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
    36001 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
    36061 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
    36121 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
    36181 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
    36241 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
    36301 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
    36361 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
    36421 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
    36481 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
    36541 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
    36601 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
    36661 cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctccttt
    36721 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
    36781 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
    36841 caccgccgcc agttccagcg cgatatcacc atctccagca tactcccagt cgatgagttt
    36901 taaccctgat gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
    36961 gcgtaaaggt cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
    37021 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
    37081 tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt
    37141 atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag
    37201 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
    37261 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
    37321 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
    37381 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
    37441 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
    37501 gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
    37561 tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta
    37621 actgttgctt agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag
    37681 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
    37741 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
    37801 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
    37861 gtgccgttcg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
    37921 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
    37981 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
    38041 tcaaggcgta gcgactcatt tttgtcatca agattcaccc ctttacaaat atagataaag
    38101 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc
    38161 gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
    38221 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga
    38281 tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc
    38341 caataaagat gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga
    38401 acgacagccc accagcagta acaacaccag agacaccagc ccaaagcatc cttttctcat
    38461 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
    38521 tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca
    38581 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc
    38641 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc
    38701 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
    38761 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
    38821 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
    38881 gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag
    38941 caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
    39001 ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt
    39061 gcatttgcaa gcaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca
    39121 tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg
    39181 ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcata gcactggcac
    39241 tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
    39301 cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta
    39361 ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc
    39421 ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
    39481 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata
    39541 actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac
    39601 tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga
    39661 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg
    39721 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
    39781 atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc
    39841 agaataacga ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tcgggcattg
    39901 tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg
    39961 atgttaatag cccaacctag ggcggtttac cgcgctggaa tactgatggc agcagcaaca
    40021 gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
    40081 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca
    40141 cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag
    40201 cggatggtat gctggttggg ccatacagta attatggacc tggctttgat catgtcggcg
    40261 gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta
    40321 gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa
    40381 acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca
    40441 actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata
    40501 ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc
    40561 tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga
    40621 aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct
    40681 acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc
    40741 tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc
    40801 gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg
    40861 gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca
    40921 gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg
    40981 gcgcaacgcg ctatcttgca gaggataacg aaggaaacgc cgttaatcct aatctgccac
    41041 gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg
    41101 gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct
    41161 tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga
    41221 aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac gataccaaca
    41281 tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat
    41341 tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta
    41401 cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac
    41461 cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca
    41521 ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg
    41581 cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt
    41641 cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat
    41701 ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga
    41761 cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac
    41821 cgtgcgagaa cgacgtaccg tcacgcattc ccagaagaat acagattggg aatgccaggc
    41881 ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag aagaactcga
    41941 tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca
    42001 ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac
    42061 cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat
    42121 aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca
    42181 tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccagacaa cgttcgatga
    42241 aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct
    42301 ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag
    42361 ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca
    42421 gcttaatacg gtagaaacgg ttaaacatgt acgctgcaat cgcaccggag ataatccctc
    42481 cgagtacgcc agtatccgcc aagtgtttag aggcgatttc ttcagcaggt aaatgcagta
    42541 ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca
    42601 gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg
    42661 gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca
    42721 gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggt agtacggata
    42781 ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa
    42841 ttgagagtgc tcctgagtat aggtgctttt tttacgttct cacgcgtggc aaggggggag
    42901 agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
    42961 cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac
    43021 attttgacaa aacatatgtc acaatatttc aaacgcggtt attcaccgcc caattttcgg
    43081 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
    43141 tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct
    43201 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
    43261 ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag
    43321 tttccaccag taatcgatcc agggggacat aacgcgcagc atcgcacagt tgctccgcat
    43381 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
    43441 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
    43501 cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata accggcttgt
    43561 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt
    43621 ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg
    43681 ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
    43741 aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta
    43801 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
    43861 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
    43921 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
    43981 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca
    44041 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
    44101 gaaagatggt ttgccagttc ggcgaccagg cccggcacat caacattggt caccagcgca
    44161 gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccacccagtg taaacgcgcc
    44221 ggagcttgtt catgattaag ggccgccagc agcgaatacc agtcgcccga tggcacgcta
    44281 tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
    44341 agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac
    44401 attgtcactt cgcgtgaaag ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa
    44461 tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg
    44521 aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
    44581 tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctag gcgtgcgtgc
    44641 tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt
    44701 tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg acatccacga
    44761 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    44821 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct
    44881 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    44941 ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca tccatcacgg
    45001 cctccagcgg ctgggtggca tcaatggtat gaatactttt atcttgtgca gccagttcca
    45061 gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct
    45121 cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta
    45181 agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    45241 taccacgtcc tccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc
    45301 aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    45361 aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    45421 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca
    45481 tgtcgcggat acccagttgc tcgagcgtct caaccaccac attacgcgcg gtagttttgc
    45541 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    45601 ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca
    45661 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    45721 agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg
    45781 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    45841 atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    45901 aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat
    45961 gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag
    46021 gcgctatcga ccgctttcac cattttcttg tgagctcgct tgagtaacgc gacatcggtg
    46081 gtattggcgg tatacatcaa ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    46141 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata
    46201 tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc
    46261 agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc
    46321 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    46381 agccattgaa aaacccgtgg gcgattgatg atcttatcag cataaagctg ttcaccgagc
    46441 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    46501 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    46561 agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    46621 gggctaaata ttcatataac gttgctgacg aaaaggtgac atcgccacag gcacgtacgg
    46681 gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcctcca
    46741 gcgaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg atacagaatt
    46801 gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acattgccct
    46861 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    46921 gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc tcaagccgat
    46981 taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    47041 acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa
    47101 tccgcgttgc tggtccgctg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga
    47161 tcacgacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa
    47221 gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga
    47281 tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat
    47341 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa ccgttaatta
    47401 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    47461 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg ggtggaaaat
    47521 gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt
    47581 acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc
    47641 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    47701 caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    47761 tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg
    47821 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    47881 actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg cgcctgcgcc
    47941 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    48001 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc
    48061 ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg
    48121 cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg
    48181 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    48241 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    48301 tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    48361 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    48421 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    48481 ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga caattccata
    48541 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    48601 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    48661 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    48721 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    48781 aggagggaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    48841 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    48901 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    48961 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    49021 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    49081 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    49141 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    49201 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    49261 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    49321 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    49381 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    49441 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    49501 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    49561 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    49621 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    49681 tttgacagac ggaaaacaga tgaaaggtta gtttcgataa tatcgttcca ctcttcatct
    49741 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    49801 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    49861 ttcaacatca gacctttgcc gttggcacct aaataatcac tgatcgcctg agagccattt
    49921 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    49981 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    50041 ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    50101 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca
    50161 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    50221 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    50281 tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact
    50341 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    50401 ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc cagcacgctc aaccgcttct
    50461 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    50521 ccttctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    50581 gccatagcgc ccgtgccttc cggtacggct tcttgcatga acttaccgcg catctcaacc
    50641 aaacgcaccg catcagcgaa atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc
    50701 aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcgatac
    50761 agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    50821 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    50881 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    50941 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    51001 cgaaccagcg cggagcccca agtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    51061 cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    51121 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatcgacat accgagtttt
    51181 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    51241 gaacggtcaa gattattcgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    51301 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcacg
    51361 cggtcggcgt ttggcagcgt cagcaattca ccataactac cgtcggcatg cagatgggtg
    51421 gaaatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    51481 ataataatag tcccacgatc tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    51541 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    51601 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccaacatg
    51661 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    51721 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    51781 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    51841 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    51901 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    51961 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    52021 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    52081 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    52141 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    52201 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    52261 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    52321 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta acgtgacatt
    52381 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    52441 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    52501 actgtcaaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    52561 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    52621 gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc gttttgccct tttgctgatg
    52681 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    52741 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    52801 tttcaccagc tccagggcca tacgcattga actcccacga ctggcgcgga tagcttgcga
    52861 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    52921 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    52981 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    53041 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    53101 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    53161 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    53221 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    53281 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    53341 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    53401 tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca
    53461 aagaccatat ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    53521 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    53581 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    53641 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    53701 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    53761 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    53821 acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    53881 tcgagagtca gggggaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    53941 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    54001 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    54061 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    54121 ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt
    54181 ttgaatgtgc agcacctgag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt
    54241 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    54301 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    54361 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata
    54421 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    54481 acgtccattt ccgtcaccta agcgaggcgg agattgataa ttacgtgcgt aaggagcatc
    54541 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    54601 tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    54661 tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgccggat gcggttacgt
    54721 cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg
    54781 cccattatca ggcttatatg cttgtattag cgcgcgttac gcagtttttg caaacaacgc
    54841 ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta
    54901 aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc
    54961 tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac
    55021 tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg
    55081 gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg
    55141 acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc
    55201 ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt
    55261 tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctac cagcaacaca
    55321 cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac
    55381 gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg
    55441 gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc
    55501 gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt
    55561 gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc
    55621 acccgcactt cacctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat
    55681 tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt
    55741 tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg
    55801 ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac
    55861 tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg
    55921 tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt
    55981 tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga
    56041 tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg
    56101 ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt
    56161 gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag
    56221 ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg
    56281 aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat
    56341 gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg
    56401 gcagcgtctg tatgacctag atatcgaaag tccagggcac gagcagaaaa aggcaaacat
    56461 ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg
    56521 cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa
    56581 ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat
    56641 tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag
    56701 tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg
    56761 ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc
    56821 ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca
    56881 atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg
    56941 cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcct tccgcgatta
    57001 tttacgtcag gatatcggcg aaatccttat cgataacccg aaagtcctcg aactggcacg
    57061 tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg
    57121 cgagatcccg ctgttcagcc actaccagat cgagtcgcag atcgagtccg ccttccagcg
    57181 tgaagtccgt ctgccatccg gtggttccat tgttatcgac agcaccgaag cgttaacagc
    57241 catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa
    57301 caccaacctc gaagccgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg
    57361 cctgattgtt atcgacttca tcgacatgac gccagtacgc caccagcgtg cggtagaaaa
    57421 ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg
    57481 ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca
    57541 tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct
    57601 ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc
    57661 cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc
    57721 gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac
    57781 cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat
    57841 gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg
    57901 taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc
    57961 aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac
    58021 accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct
    58081 gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa
    58141 accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga
    58201 gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg
    58261 taatcgtcgc caggcacagc agcaaactgc cgagacgcgt gagagccgtc agcaggctga
    58321 ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg
    58381 ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga
    58441 gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa
    58501 acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt
    58561 cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc
    58621 acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca
    58681 agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc
    58741 gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc
    58801 aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa
    58861 agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga
    58921 acaggaagaa gtacaagtgc agccgatggt gactgaggtc cctgtcgccg ccgctgtcga
    58981 acctgttgtt agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc
    59041 ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc
    59101 ggtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagtcgc
    59161 agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc
    59221 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    59281 cgtcgctgaa gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag agatcaccgt
    59341 tgagcataac cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga
    59401 ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    59461 aggtggtcat acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    59521 gtaataatta gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt
    59581 ttgaatcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag
    59641 gcaaaaaaaa tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca
    59701 tgatatgttt taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg
    59761 gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt
    59821 gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg
    59881 ctacctaagg aatccagcga ctccagtttg ttcagctgcg tgcctaattc cgtgcgaacg
    59941 gtaaggacat tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg
    60001 gcgatttctt tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta
    60061 tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta
    60121 ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca
    60181 tcgacctgtt gcttaatact ttctgttccg ccgacataat caccgttagc ctcactaaac
    60241 ggcgcagtct ctgttttata accggcaaaa atgtagcgcc cattaccgtc agtggtgttc
    60301 gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca
    60361 tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca
    60421 gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt
    60481 gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca
    60541 gcaatgggat cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac
    60601 ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg
    60661 gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt
    60721 ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc
    60781 cgtattcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca
    60841 ccacattacc ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac
    60901 tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc
    60961 tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc
    61021 tcgccatcgc gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac
    61081 ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca
    61141 gaccatcaaa tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg
    61201 caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta
    61261 ccacggaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt
    61321 gctgaaccgc gggattaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa
    61381 atccggcttt gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg
    61441 tattacgcgt ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg
    61501 tattcagtaa tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag
    61561 tcgtacgaga agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct
    61621 gaaccagtga gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac
    61681 ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    61741 gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc
    61801 aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    61861 tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga
    61921 tttcccaatc agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag
    61981 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    62041 gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcacagc
    62101 acgtaactgg ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc
    62161 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    62221 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    62281 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    62341 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    62401 agattatcaa tgttcatgct gtaggttttg ctcacctggt cgcttatcga tttcatctgc
    62461 tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt
    62521 aggacctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    62581 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    62641 ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact
    62701 tgccctttcc agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt
    62761 tcgcggcgga tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa
    62821 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    62881 aatgctctac tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc
    62941 agctgcgaaa gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    63001 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc
    63061 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata
    63121 ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    63181 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    63241 tttgccgccg gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    63301 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    63361 gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca
    63421 gcgcattgag cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    63481 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac
    63541 caaacggtgt atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc
    63601 cctgcgctac cgcgcagctg tcgagggtca cttctcgatt catcaccacc gaaccggtgc
    63661 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    63721 cggcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg
    63781 catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    63841 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    63901 ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt
    63961 taacctgaac actgctaccg cccgcggagg ctcctgcgcc gccaaccaga atattgccct
    64021 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc
    64081 cacgcaagct tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac
    64141 gtccaaacgg aggaagtgac actgtcacca ttaccacagc gacgtttttt agctgcatat
    64201 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    64261 taaacggcgt ctgggttgtc tggtcaccag tgccatccag ccccactacc aggccatagc
    64321 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    64381 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    64441 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    64501 gttaatgtag ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac
    64561 ggtattgctg ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt
    64621 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    64681 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    64741 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    64801 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    64861 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    64921 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    64981 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    65041 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    65101 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    65161 tcagtaaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    65221 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    65281 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    65341 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    65401 tgtagaggtt ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga
    65461 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    65521 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    65581 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg
    65641 ctgatgagcc atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt
    65701 tgctgttatt ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc
    65761 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    65821 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    65881 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    65941 caaggcccgt tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct
    66001 gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca
    66061 aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact
    66121 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    66181 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    66241 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    66301 ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    66361 ttcgctgccc gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg
    66421 atcgcccgga ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc
    66481 cggcacagca attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc
    66541 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    66601 cacggccagc catcctgctg caacgcaacg tccagcgggc gcgaggtgta atccatcttg
    66661 ccgggcgtca tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa
    66721 agcccttcca ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag
    66781 gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc
    66841 atcgcagtat aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac
    66901 ccgcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact gatagttacg
    66961 ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc
    67021 accgttggtc agggtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga
    67081 agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa
    67141 gttcgccagt acaatctgcc ccagcagttg ggtttgttcg ttggaatagt tgccgacaac
    67201 cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt
    67261 ggttgccaca atgttgttag cgccggtatt ttgctgcatg gagttgagga agctcagact
    67321 aaacgtggcg ggttctgcac cgttaattgc gccggttgcg atattattcg ccatcgcaca
    67381 tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    67441 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat
    67501 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    67561 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    67621 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    67681 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag
    67741 ctgtaaacct tgcatattca acaggttacg gttttcatcc agcttaaatt gtccgttacg
    67801 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    67861 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    67921 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    67981 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    68041 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    68101 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    68161 atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    68221 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    68281 cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt
    68341 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    68401 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    68461 ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat
    68521 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    68581 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    68641 gttgttttcc attggattgg tcgggtccta gtttttcagc tgcgccacca gcaaagtcag
    68701 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    68761 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    68821 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    68881 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    68941 ggcattttta cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg
    69001 tccggggcct gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    69061 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    69121 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    69181 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    69241 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    69301 ggctgttatc ggcaaactgg gtgacttcct gacgctgggc gcgcagattg agcgcctctt
    69361 gttgaaaacg taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat
    69421 tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg
    69481 tcgctattta tgccgttgat ggtcattgcg gacaggtaca actcacgttg tagaaatggc
    69541 tgggggcgaa aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc
    69601 cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg
    69661 cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat
    69721 gcgagcagcc attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt
    69781 tggcacgctg cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt
    69841 atgtggttgc cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac
    69901 tgaaacgtgg acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg
    69961 ttgatgccgt tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc
    70021 gccaggcatg gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt
    70081 ttagcgccaa cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg
    70141 tgcgcatggt atcgggacag gtagtcagcg gcgttgttga tgcagatggg aatattctta
    70201 taaacctgta agctgttaaa gattacccgt cccttgccga taaataagca acacatgata
    70261 aaagcgccct caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg
    70321 taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt aacgaacagc cgcctccacc
    70381 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    70441 atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg
    70501 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    70561 tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    70621 caaaacggta atggatcatg agcagcaaca tctctctatg gggcagatca acggcagcca
    70681 gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca
    70741 gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga
    70801 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    70861 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    70921 gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat
    70981 ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg
    71041 caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg
    71101 ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg
    71161 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    71221 gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc
    71281 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    71341 gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    71401 caattttcac tggcgtttta atgtcttggc gggaataaaa gccaggagcc aacactttca
    71461 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    71521 catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    71581 tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca
    71641 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    71701 aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt
    71761 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    71821 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    71881 ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    71941 gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca
    72001 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa
    72061 gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    72121 ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt aggtggctgg
    72181 tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    72241 gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa
    72301 cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa
    72361 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa
    72421 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    72481 ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    72541 gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg
    72601 caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt
    72661 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc
    72721 actgcctatt cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc
    72781 cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta
    72841 ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    72901 gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa
    72961 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    73021 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    73081 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    73141 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    73201 tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    73261 gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    73321 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    73381 gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga
    73441 atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt
    73501 aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    73561 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga
    73621 atcaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa
    73681 atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact
    73741 gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa
    73801 aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    73861 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc agcgtctggg caccacgcaa
    73921 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    73981 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    74041 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    74101 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    74161 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag
    74221 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    74281 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    74341 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    74401 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    74461 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    74521 agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt
    74581 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    74641 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    74701 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    74761 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    74821 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    74881 attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat
    74941 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc
    75001 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    75061 cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca
    75121 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    75181 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt
    75241 ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac
    75301 cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc
    75361 acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg
    75421 atccgccgcg ttcggcgttg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt
    75481 tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct
    75541 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat
    75601 gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    75661 ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc
    75721 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    75781 acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    75841 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaataccggt
    75901 tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc
    75961 tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg
    76021 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    76081 ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt
    76141 ttgacctggg caaatcgacg caccagccag agcttccgtg gatgatgctg ggaattattg
    76201 gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt
    76261 tgctggaacg cgacgcctga tttgatacac ccccctgact cctccatact gaaagtgaac
    76321 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    76381 tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca
    76441 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    76501 aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg
    76561 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    76621 tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    76681 tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt
    76741 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg
    76801 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    76861 ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    76921 gcgttgctga ttaccacgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    76981 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    77041 ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    77101 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    77161 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    77221 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    77281 gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag
    77341 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg
    77401 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc
    77461 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    77521 caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct
    77581 tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc
    77641 attgaagtga agccaggtga aattgtcatt ccgtttacgg attaatgacg agggcgcatt
    77701 cgcgcccttt atttttcgtg caaaggaaaa catttccgct tatcctttgt gtccggcaaa
    77761 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    77821 cgccgtccag acgactggca ccttcacctc cgcgatggcg acatgttaca aactgtcgtg
    77881 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    77941 accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg
    78001 cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg
    78061 gagcgcggat ttaatgaagg cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc
    78121 actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg
    78181 gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    78241 tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact
    78301 gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac
    78361 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    78421 atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt
    78481 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg
    78541 gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc
    78601 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    78661 cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac
    78721 acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat
    78781 gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    78841 tcgtagcttc ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca
    78901 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctgg
//
LOCUS       DADLTI010000004        65605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLTI010000004 DADLTI010000000
VERSION     DADLTI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.004"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..146
                     /locus_tag="JN755_000265"
     CDS             <1..146
                     /locus_tag="JN755_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7379183.1"
                     /translation="QHKLLQTARLWLARHNGSFDTVDCRFDVVAFTGNEVEWIKDAFN
                     DHS"
     gene            166..756
                     /gene="diaA"
                     /locus_tag="JN755_000266"
     CDS             166..756
                     /gene="diaA"
                     /locus_tag="JN755_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7379184.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            766..1341
                     /gene="yraP"
                     /locus_tag="JN755_000267"
     CDS             766..1341
                     /gene="yraP"
                     /locus_tag="JN755_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7379185.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(1455..2495)
                     /locus_tag="JN755_000268"
     CDS             complement(1455..2495)
                     /locus_tag="JN755_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7379186.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(2568..3203)
                     /locus_tag="JN755_000269"
     CDS             complement(2568..3203)
                     /locus_tag="JN755_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7379187.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            3331..3849
                     /gene="yhbO"
                     /locus_tag="JN755_000270"
     CDS             3331..3849
                     /gene="yhbO"
                     /locus_tag="JN755_000270"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7379188.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(3829..4272)
                     /locus_tag="JN755_000271"
     CDS             complement(3829..4272)
                     /locus_tag="JN755_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY7379189.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN755_000272"
     CDS             4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN755_000272"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7379190.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(4612..5115)
                     /locus_tag="JN755_000273"
     CDS             complement(4612..5115)
                     /locus_tag="JN755_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7379191.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(5109..5633)
                     /locus_tag="JN755_000274"
     CDS             complement(5109..5633)
                     /locus_tag="JN755_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7379192.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            5842..6837
                     /locus_tag="JN755_000275"
     CDS             5842..6837
                     /locus_tag="JN755_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7379193.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            6846..7724
                     /locus_tag="JN755_000276"
     CDS             6846..7724
                     /locus_tag="JN755_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7379194.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            7805..8812
                     /locus_tag="JN755_000277"
     CDS             7805..8812
                     /locus_tag="JN755_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY7379195.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(8930..10174)
                     /gene="mtr"
                     /locus_tag="JN755_000278"
     CDS             complement(8930..10174)
                     /gene="mtr"
                     /locus_tag="JN755_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7379196.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(10328..12217)
                     /locus_tag="JN755_000279"
     CDS             complement(10328..12217)
                     /locus_tag="JN755_000279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7379197.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(12210..12290)
                     /gene="yrbN"
                     /locus_tag="JN755_000280"
     CDS             complement(12210..12290)
                     /gene="yrbN"
                     /locus_tag="JN755_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7379198.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(12397..13281)
                     /gene="nlpI"
                     /locus_tag="JN755_000281"
     CDS             complement(12397..13281)
                     /gene="nlpI"
                     /locus_tag="JN755_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7379199.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(13390..15525)
                     /gene="pnp"
                     /locus_tag="JN755_000282"
     CDS             complement(13390..15525)
                     /gene="pnp"
                     /locus_tag="JN755_000282"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7379200.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(15772..16041)
                     /gene="rpsO"
                     /locus_tag="JN755_000283"
     CDS             complement(15772..16041)
                     /gene="rpsO"
                     /locus_tag="JN755_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7379201.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(16190..17134)
                     /gene="truB"
                     /locus_tag="JN755_000284"
     CDS             complement(16190..17134)
                     /gene="truB"
                     /locus_tag="JN755_000284"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7379202.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(17134..17535)
                     /gene="rbfA"
                     /locus_tag="JN755_000285"
     CDS             complement(17134..17535)
                     /gene="rbfA"
                     /locus_tag="JN755_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7379203.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(17699..20347)
                     /gene="infB"
                     /locus_tag="JN755_000286"
     CDS             complement(17699..20347)
                     /gene="infB"
                     /locus_tag="JN755_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7379204.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(20372..21859)
                     /gene="nusA"
                     /locus_tag="JN755_000287"
     CDS             complement(20372..21859)
                     /gene="nusA"
                     /locus_tag="JN755_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7379205.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(21887..22339)
                     /gene="rimP"
                     /locus_tag="JN755_000288"
     CDS             complement(21887..22339)
                     /gene="rimP"
                     /locus_tag="JN755_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7379206.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(22546..22622)
                     /locus_tag="JN755_000289"
     tRNA            complement(22546..22622)
                     /locus_tag="JN755_000289"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22586..22588),aa:Met,seq:cat)
     gene            22970..24313
                     /gene="argG"
                     /locus_tag="JN755_000290"
     CDS             22970..24313
                     /gene="argG"
                     /locus_tag="JN755_000290"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7379207.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(24321..25945)
                     /locus_tag="JN755_000291"
                     /pseudo
     CDS             complement(24321..25945)
                     /locus_tag="JN755_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(26404..26490)
                     /locus_tag="JN755_000292"
     tRNA            complement(26404..26490)
                     /locus_tag="JN755_000292"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26454..26456),aa:Leu,seq:gag)
     gene            complement(26505..26837)
                     /gene="secG"
                     /locus_tag="JN755_000293"
     CDS             complement(26505..26837)
                     /gene="secG"
                     /locus_tag="JN755_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7379208.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(27065..28402)
                     /gene="glmM"
                     /locus_tag="JN755_000294"
     CDS             complement(27065..28402)
                     /gene="glmM"
                     /locus_tag="JN755_000294"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7379209.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(28395..29243)
                     /gene="folP"
                     /locus_tag="JN755_000295"
     CDS             complement(28395..29243)
                     /gene="folP"
                     /locus_tag="JN755_000295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7379210.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(29333..31267)
                     /gene="ftsH"
                     /locus_tag="JN755_000296"
     CDS             complement(29333..31267)
                     /gene="ftsH"
                     /locus_tag="JN755_000296"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7379211.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(31367..31996)
                     /gene="rlmE"
                     /locus_tag="JN755_000297"
     CDS             complement(31367..31996)
                     /gene="rlmE"
                     /locus_tag="JN755_000297"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7379212.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVNQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            32122..32415
                     /gene="yhbY"
                     /locus_tag="JN755_000298"
     CDS             32122..32415
                     /gene="yhbY"
                     /locus_tag="JN755_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7379213.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(32571..33047)
                     /gene="greA"
                     /locus_tag="JN755_000299"
     CDS             complement(32571..33047)
                     /gene="greA"
                     /locus_tag="JN755_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7379214.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            33295..34728
                     /gene="dacB"
                     /locus_tag="JN755_000300"
     CDS             33295..34728
                     /gene="dacB"
                     /locus_tag="JN755_000300"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7379215.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKMITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(34914..36086)
                     /gene="cgtA"
                     /locus_tag="JN755_000301"
     CDS             complement(34914..36086)
                     /gene="cgtA"
                     /locus_tag="JN755_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7379216.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(36102..37067)
                     /locus_tag="JN755_000302"
     CDS             complement(36102..37067)
                     /locus_tag="JN755_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7379217.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(37194..37451)
                     /gene="rpmA"
                     /locus_tag="JN755_000303"
     CDS             complement(37194..37451)
                     /gene="rpmA"
                     /locus_tag="JN755_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7379218.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(37472..37783)
                     /gene="rplU"
                     /locus_tag="JN755_000304"
     CDS             complement(37472..37783)
                     /gene="rplU"
                     /locus_tag="JN755_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7379219.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            38042..39013
                     /gene="ispB"
                     /locus_tag="JN755_000305"
     CDS             38042..39013
                     /gene="ispB"
                     /locus_tag="JN755_000305"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7379220.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            39241..39519
                     /gene="sfsB"
                     /locus_tag="JN755_000306"
     CDS             39241..39519
                     /gene="sfsB"
                     /locus_tag="JN755_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7379221.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(39567..40826)
                     /gene="murA"
                     /locus_tag="JN755_000307"
     CDS             complement(39567..40826)
                     /gene="murA"
                     /locus_tag="JN755_000307"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7379222.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(40881..41135)
                     /gene="ibaG"
                     /locus_tag="JN755_000308"
     CDS             complement(40881..41135)
                     /gene="ibaG"
                     /locus_tag="JN755_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7379223.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(41295..41588)
                     /gene="mlaB"
                     /locus_tag="JN755_000309"
     CDS             complement(41295..41588)
                     /gene="mlaB"
                     /locus_tag="JN755_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7379224.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(41588..42223)
                     /gene="mlaC"
                     /locus_tag="JN755_000310"
     CDS             complement(41588..42223)
                     /gene="mlaC"
                     /locus_tag="JN755_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7379225.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(42242..42793)
                     /gene="mlaD"
                     /locus_tag="JN755_000311"
     CDS             complement(42242..42793)
                     /gene="mlaD"
                     /locus_tag="JN755_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7379226.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(42798..43580)
                     /gene="mlaE"
                     /locus_tag="JN755_000312"
     CDS             complement(42798..43580)
                     /gene="mlaE"
                     /locus_tag="JN755_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7379227.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(43588..44397)
                     /gene="mlaF"
                     /locus_tag="JN755_000313"
     CDS             complement(43588..44397)
                     /gene="mlaF"
                     /locus_tag="JN755_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7379228.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            44607..45572
                     /locus_tag="JN755_000314"
     CDS             44607..45572
                     /locus_tag="JN755_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7379229.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            45586..46572
                     /gene="kdsD"
                     /locus_tag="JN755_000315"
     CDS             45586..46572
                     /gene="kdsD"
                     /locus_tag="JN755_000315"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7379230.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            46593..47159
                     /gene="kdsC"
                     /locus_tag="JN755_000316"
     CDS             46593..47159
                     /gene="kdsC"
                     /locus_tag="JN755_000316"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7379231.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            47156..47731
                     /gene="lptC"
                     /locus_tag="JN755_000317"
     CDS             47156..47731
                     /gene="lptC"
                     /locus_tag="JN755_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7379232.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            47700..48257
                     /gene="lptA"
                     /locus_tag="JN755_000318"
     CDS             47700..48257
                     /gene="lptA"
                     /locus_tag="JN755_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7379233.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVILTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            48264..48989
                     /gene="lptB"
                     /locus_tag="JN755_000319"
     CDS             48264..48989
                     /gene="lptB"
                     /locus_tag="JN755_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7379234.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            49037..50470
                     /gene="rpoN"
                     /locus_tag="JN755_000320"
     CDS             49037..50470
                     /gene="rpoN"
                     /locus_tag="JN755_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7379235.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            50493..50780
                     /gene="hpf"
                     /locus_tag="JN755_000321"
     CDS             50493..50780
                     /gene="hpf"
                     /locus_tag="JN755_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7379236.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            50898..51389
                     /gene="ptsN"
                     /locus_tag="JN755_000322"
     CDS             50898..51389
                     /gene="ptsN"
                     /locus_tag="JN755_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7379237.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            51435..52289
                     /gene="rapZ"
                     /locus_tag="JN755_000323"
     CDS             51435..52289
                     /gene="rapZ"
                     /locus_tag="JN755_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7379238.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            52286..52558
                     /gene="npr"
                     /locus_tag="JN755_000324"
     CDS             52286..52558
                     /gene="npr"
                     /locus_tag="JN755_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7379239.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            52771..53403
                     /gene="yrbL"
                     /locus_tag="JN755_000325"
     CDS             52771..53403
                     /gene="yrbL"
                     /locus_tag="JN755_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7379240.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(53400..54128)
                     /gene="mtgA"
                     /locus_tag="JN755_000326"
     CDS             complement(53400..54128)
                     /gene="mtgA"
                     /locus_tag="JN755_000326"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7379241.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(54125..54778)
                     /gene="elbB"
                     /locus_tag="JN755_000327"
     CDS             complement(54125..54778)
                     /gene="elbB"
                     /locus_tag="JN755_000327"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7379242.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(55008..57344)
                     /gene="arcB"
                     /locus_tag="JN755_000328"
     CDS             complement(55008..57344)
                     /gene="arcB"
                     /locus_tag="JN755_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7379243.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(57440..58369)
                     /locus_tag="JN755_000329"
     CDS             complement(57440..58369)
                     /locus_tag="JN755_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7379244.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            59044..63504
                     /gene="gltB"
                     /locus_tag="JN755_000330"
     CDS             59044..63504
                     /gene="gltB"
                     /locus_tag="JN755_000330"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7379245.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            63517..64935
                     /locus_tag="JN755_000331"
     CDS             63517..64935
                     /locus_tag="JN755_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7379246.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            65119..65598
                     /locus_tag="JN755_000332"
     CDS             65119..65598
                     /locus_tag="JN755_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7379247.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16042 a        17825 c        16231 g        15507 t
ORIGIN      
        1 aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg
       61 atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta
      121 aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt
      181 aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat
      241 gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc
      301 tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac
      361 cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc
      421 ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg
      481 ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt
      541 gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat
      601 gacggcggcg aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg
      661 catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg
      721 atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg
      781 ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg
      841 ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg
      901 gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag
      961 aaagaaaccc gtattaatgt cacggcctat cagggcaaag tgctgctggt tgggcagtca
     1021 ccaaatgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac
     1081 gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat
     1141 acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc
     1201 aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt aactgaacgt
     1261 gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggtaaccaca
     1321 gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct
     1381 tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca
     1441 tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc agacactgcc
     1501 accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt
     1561 gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata
     1621 cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag
     1681 catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca
     1741 tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg
     1801 atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt
     1861 gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt
     1921 tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca
     1981 aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg
     2041 cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat tcccgccgcg
     2101 accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccggcagc
     2161 gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc
     2221 tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc gaggaaatag
     2281 atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc aaggatagat
     2341 ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg ttgccatttc
     2401 acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg
     2461 ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga
     2521 taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt
     2581 ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg catcgcctct
     2641 gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc tggtaacaaa
     2701 cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac
     2761 atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc
     2821 tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc
     2881 accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg
     2941 taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa
     3001 caaaatacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca
     3061 tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc agcaatggcg
     3121 ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg
     3181 cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc ttgcgctccc
     3241 cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt ggaaaattcg
     3301 cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcact
     3361 gatgaatttg aagattcaga atttacttca cccgcagacg agttccgtaa agccggacac
     3421 gaagtgatta ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc
     3481 agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgttg
     3541 ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc
     3601 cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg
     3661 atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat
     3721 gttaaaaatg cgggcgtgga attttacgat caggaagtcg tggttgataa agatcagctg
     3781 gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc
     3841 ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa
     3901 tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa
     3961 gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc
     4021 acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc
     4081 ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat
     4141 gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc agagttcccc
     4201 ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc tgatggcgat
     4261 gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat gcgatgcaga
     4321 cgatgacccc ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga
     4381 tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac
     4441 tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg
     4501 cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag
     4561 cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag attaaaagcg
     4621 attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa
     4681 acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc
     4741 cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc
     4801 atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg
     4861 tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc
     4921 ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc
     4981 caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc
     5041 atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc
     5101 tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt
     5161 cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc
     5221 tccagttcaa tggcgtccat caggtttttc acatacaccc ccagctccgt atcgccttca
     5281 atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc
     5341 agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg gctgaccacc
     5401 agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacacac atgaatactt
     5461 aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg gaattgccag
     5521 cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca
     5581 ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt
     5641 actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa
     5701 atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca
     5761 aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata
     5821 gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt
     5881 aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat
     5941 gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt
     6001 gtccatcaac atcgccgcaa acttcacatc tcgattaaca cttttgcgca tccggacggt
     6061 tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc
     6121 ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat
     6181 gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt
     6241 gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca
     6301 cgggttactc ctgtcccgct ggaagtgttt gctttcggca gcctgtgcat tatgtcggaa
     6361 ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt
     6421 tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac
     6481 gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa
     6541 gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat
     6601 accctggaac tgctgccgga gttaatggct gcgaatattg cttcggtgaa aattgaaggc
     6661 cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac
     6721 cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg
     6781 tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa
     6841 gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag acgctggaag
     6901 atttttatca gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt gaagcggtat
     6961 gcagcaagcg ccgggcaacc aaagttggcg actgtctgga gatggcaaaa tcgctcgccg
     7021 gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc tctgaactgg
     7081 gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc gatctcggcg
     7141 tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct
     7201 acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg
     7261 tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc
     7321 gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc tactccgccc
     7381 gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta
     7441 agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg
     7501 gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca tccatgcagg
     7561 gattggttga tgtagtccgc ctgtcaccgc agggcactga cactttcgcg atgctcgacg
     7621 ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac agcgactgta
     7681 acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat agctcacttt
     7741 gttaacaact ttaactattc tttaatgcag tattaaagat taatcggtaa caaagtgagc
     7801 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca tacccgaagg
     7861 ctcttcagcg cgggaagcgt tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
     7921 cggctatcat cgctactggc tggcagaaca ccacaatatg accggcattg ccagtgctgc
     7981 aacatcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
     8041 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacactcaa
     8101 tacgctctat ccggggcgaa tcgatttggg gctgggtcgt gctccgggta gtgaccaacg
     8161 gacaatgatg gcgctacgtc gtcatatgag cggcgatatt gataatttcc cccgcgatgt
     8221 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
     8281 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gtgcacaact
     8341 ggcggcgcag cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt
     8401 ccaggcgctg catctttatc gcagtaactt caaaccgtcg gctcggctgg aaaaaccata
     8461 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt
     8521 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc
     8581 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatgacg tgcagcaggc
     8641 gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt catggtttgc agtcgatcct
     8701 gcgcgaaacc gacgctgatg agattatggt taacgggcag attttcgacc accaggcgcg
     8761 cctgcattcg tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt
     8821 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtaggcctga taagcgaagc
     8881 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
     8941 ccggcagtaa attaaagctc gataaaatat gcaccagtgc gttgccgacg ccaaacacca
     9001 gaatcagcgc aatcatcggc ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt
     9061 tacgcgatgc gcgggccagc agtgccggaa caattgccgc ccagatggta gccgctaaac
     9121 cagcataacc aatggcatac aggaatccgt tcgggaacag cagaccaccc acaacaggcg
     9181 gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca
     9241 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
     9301 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
     9361 cttgtaccag cacatcaata ttaccgccct tctcggcgat gccgataaac tccggacgag
     9421 ggatgttgcc cattgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
     9481 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggcttg
     9541 gcacgttacc gtgataacca aacgacgcca agcagaacgg cagggtcatc agcaaatacg
     9601 gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca
     9661 gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca atcgccgtca
     9721 tgcggctgac ggctttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
     9781 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaatcg
     9841 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatgc
     9901 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
     9961 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    10021 aggtaaagat cagcgctgcc attgaccaga aaaaccacgc cccggacatg accactggca
    10081 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    10141 acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag tgaaaaatag
    10201 tgcgactgcg ttgttatgca ttgcactgta ccagtacatg agtacaaaag acagaaaaaa
    10261 agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    10321 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggtg
    10381 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacgacca ccttcacggc
    10441 gttcaccgct gaagttacga ccgccttcac gacgctcgcc accgaaacca cgaccaccgc
    10501 cacgaagctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    10561 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    10621 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt
    10681 cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc aacaccatca tcgcggccca
    10741 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    10801 cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    10861 ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta cgttcaccct
    10921 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    10981 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    11041 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    11101 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    11161 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    11221 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc aggctgatac
    11281 gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt
    11341 ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    11401 cgctattgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    11461 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    11521 ttttacgcat accccacaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    11581 tggactgaat acgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    11641 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    11701 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    11761 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    11821 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    11881 ggccgccgta cagggcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    11941 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    12001 tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc
    12061 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    12121 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    12181 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    12241 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    12301 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    12361 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    12421 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    12481 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    12541 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    12601 ggtgttatcg gttgcgtccg ccttgagcct ttccattaac gtgtgttcgc taatgttgcc
    12661 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    12721 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    12781 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    12841 cttgtcacga ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt
    12901 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    12961 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    13021 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    13081 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    13141 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    13201 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    13261 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    13321 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    13381 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    13441 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttccagaac
    13501 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    13561 agcgatttga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    13621 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    13681 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt
    13741 cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca gggcacggat
    13801 aacagagccg cctttaccga taacgtcttt gatcttgtcc gggttgatct tgatggtatg
    13861 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    13921 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    13981 ggtgatacct tcaattttga tatccatctg caatgcagag ataccctcgc gggaacccgc
    14041 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    14101 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgatcgg
    14161 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaggaacc
    14221 gttggattca gtgatttcag acacaacacg tacggtgtac gggaatttgt ccatatccgg
    14281 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    14341 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    14401 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cagtacccag
    14461 cgttgcggta accagcgcct gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg
    14521 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    14581 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat
    14641 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    14701 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    14761 gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    14821 cggctgccag tcccaacgtg gtttaccggc ttctttcacc agttcattga tgttctgaat
    14881 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctggt cttcgctcag
    14941 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    15001 cagtttgctc tctttcaact cgtcctgagt cgggttcagt acgtactggt cattgatgta
    15061 acctacgcga gcagcaccaa tcggaccatt gaacggaata ccagacagag acagtgctgc
    15121 ggaagcacca atcatcgcga cgatatccgg gttaacttgc gggttaacag aaaccacggt
    15181 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    15241 aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac ggaagaagct
    15301 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    15361 gtcctgacct ggtttggctt ttttctggcc aacaacggta acaaataccg cggtgtcatc
    15421 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    15481 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    15541 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    15601 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    15661 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    15721 cagcttgaaa aaaggggcca ctcaggcccc tttttctgaa actcgcaaga attagcgacg
    15781 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    15841 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    15901 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    15961 aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    16021 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    16081 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    16141 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    16201 attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttca cccataccga
    16261 taaatttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    16321 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    16381 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    16441 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcacaatcc
    16501 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    16561 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    16621 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    16681 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    16741 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    16801 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    16861 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    16921 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgccag
    16981 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    17041 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    17101 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    17161 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    17221 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    17281 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    17341 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    17401 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    17461 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    17521 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    17581 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    17641 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    17701 aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg ccagtgcgga
    17761 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    17821 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    17881 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    17941 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    18001 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    18061 tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt gcagaggcat
    18121 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    18181 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    18241 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    18301 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    18361 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    18421 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    18481 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    18541 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    18601 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    18661 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    18721 gcagcaggat agcgtccagc agttcatcga taccagtacc cgctttcgca gacacatgta
    18781 cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag agttcgttct
    18841 taacgcgatc cggatcggct tctggtttat cgatcttgtt cactgcaacg accaccggaa
    18901 cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg
    18961 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc attgaagtaa
    19021 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    19081 atgcaccaat gtgctgagta atgccgcccg cttcgccaga ggccactttc gttgaacgaa
    19141 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    19201 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    19261 tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt gcggtttcct
    19321 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    19381 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca
    19441 caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc gaagaacctt
    19501 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    19561 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    19621 ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    19681 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    19741 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg
    19801 cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    19861 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    19921 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca cgttcagctt
    19981 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctg
    20041 caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga
    20101 tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac
    20161 gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct
    20221 gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt
    20281 gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat
    20341 ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat
    20401 tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca
    20461 gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt
    20521 tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat
    20581 caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc
    20641 gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca
    20701 cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc gcgaagtctt
    20761 cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct
    20821 tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc tgcgaagcca
    20881 gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga
    20941 tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg
    21001 cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc
    21061 ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg
    21121 gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc gggtcgcgag
    21181 ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaata cggaacagtt
    21241 cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc
    21301 gaacggaata aagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca
    21361 tatcttcgcg caggatcacg gcttcagcat tgttgcccag atccagagag atgttgtcgc
    21421 ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa
    21481 ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg
    21541 tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaagttca
    21601 ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt
    21661 catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta
    21721 cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc gcgctttcca
    21781 atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag
    21841 ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa
    21901 ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa
    21961 ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta
    22021 ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg
    22081 tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg
    22141 gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat
    22201 tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt
    22261 cgatgcctac cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt
    22321 tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt
    22381 attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta
    22441 taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag
    22501 gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg
    22561 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccagactg ctccaccccg
    22621 cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt
    22681 tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg ccacattttg
    22741 tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact
    22801 tttgccttcc cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc
    22861 ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga
    22921 gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat
    22981 tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac
    23041 cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa
    23101 cctaggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg
    23161 cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc
    23221 tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc
    23281 gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa
    23341 tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg
    23401 tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga
    23461 tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa
    23521 aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcatgaagc
    23581 gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa
    23641 attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg
    23701 tcatccggtt gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc
    23761 taaccgcatc ggcggtcgtc acggcttggg catgagcgac cagattgaga accgtatcat
    23821 cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc acattgcgta
    23881 tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg
    23941 tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg cgctgatgct
    24001 gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct
    24061 gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc
    24121 agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca
    24181 attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg gttatgccaa
    24241 aactggcctg ctttcctcct ctgccacttc aggcctgccg caggtggaga atatggaaaa
    24301 caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc
    24361 tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc
    24421 cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg ttccggcttt
    24481 gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt
    24541 tccgttgtgc gatccacgcc atccccaaga acggggctat aacagataaa catcgggaca
    24601 tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg
    24661 tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat
    24721 tcaaaaaact gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc
    24781 tgatcgtcct gcggttgaaa cacgcgctgg attgcggata ggcgctacaa gccggttcat
    24841 ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt
    24901 gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcgc
    24961 gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc
    25021 agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa
    25081 tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag
    25141 gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg
    25201 gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca
    25261 caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa tacggcaccg
    25321 tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa tagttgagat
    25381 tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg aacgcttttt
    25441 tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc gaaatgacaa
    25501 tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta
    25561 ctgccaaaaa aaaagggata aaaaagcaaa agcacataga taagggatat acatccccag
    25621 cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa
    25681 agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa
    25741 cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag
    25801 gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa
    25861 gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt taaaatcttc
    25921 tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt
    25981 atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgaaccat atcagaccga
    26041 aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt aattaaataa
    26101 cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt atactgacag
    26161 gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac tggagttgac
    26221 acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg cgtaccggca
    26281 ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag atacctgaca
    26341 accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct cttttctgga
    26401 atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg
    26461 tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt cgggatatcg
    26521 ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg tgcactcaga
    26581 ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag caccagactg
    26641 atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt accagaacca
    26701 cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct
    26761 ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag gaaaactact
    26821 aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca
    26881 accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata ctaaccaaag
    26941 cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa aaaacagcaa
    27001 aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc agttatctaa
    27061 cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt
    27121 cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta cgcagcaaca
    27181 cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta actgactcat
    27241 gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca
    27301 ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc gccgccagca
    27361 cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg atcacatgac
    27421 cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt
    27481 cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt tcaagcccca
    27541 tgttgctcat caatgtaccc acagcgccac cacgcagctg gccctgacga agaccttcac
    27601 gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg tcaaccataa
    27661 tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac
    27721 gagcctggag cacgcgaacg tcggtagccc ccacttcggc attgatgttt acaccgtttg
    27781 gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt
    27841 gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg ctaagttcgt
    27901 tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg atacggctgg
    27961 ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg
    28021 cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta atcccattat
    28081 cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg aaggtacgcg
    28141 tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg
    28201 ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac
    28261 caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc caacccagct
    28321 taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac
    28381 cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt ggcttccacc
    28441 acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc
    28501 attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc
    28561 agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg
    28621 tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg
    28681 aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt
    28741 tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg agcttcctgc
    28801 atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc
    28861 gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg agcgccaact
    28921 ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca gacttcgaag
    28981 cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc aacgctaact
    29041 tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg
    29101 atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc accatccgaa
    29161 aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa
    29221 gtaccctggg caaagagttt catgatgtta tccctggtat gaattgataa gaaaaaaccc
    29281 cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga acttacttgt
    29341 cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac
    29401 gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt tcttcccagc
    29461 ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag
    29521 tctcatattt catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa
    29581 gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac
    29641 gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg aatacttcac
    29701 cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac tgagtcacca
    29761 tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta gaaacatgtt
    29821 ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa atctggcttt
    29881 ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca
    29941 gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc ggcaccaggc
    30001 gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc gcttccgtca
    30061 tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa
    30121 ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt
    30181 tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc gcgtcgatat
    30241 cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat
    30301 ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg gccgggtcga
    30361 gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct tcgaagccat
    30421 ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccagac
    30481 cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg
    30541 ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt
    30601 ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaatcgctt
    30661 tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct ttcgggatct
    30721 taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt tcagcaactt
    30781 cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg
    30841 tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg ccgccctgca
    30901 tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac caggagatga
    30961 agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg acgttcttgg
    31021 tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac
    31081 tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt
    31141 tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc
    31201 caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt
    31261 tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga
    31321 tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc
    31381 cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc ttacgaactt
    31441 tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga
    31501 acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt tctagcgcca
    31561 gttccaccag atacatggca cgggggatat ccaccgccgg tgttccgctc atgtttggtg
    31621 ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca
    31681 tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag
    31741 gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttggttgacc acatattgtg
    31801 accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt
    31861 cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc tttttctgtg
    31921 cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac
    31981 gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa
    32041 tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc
    32101 ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt
    32161 ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg
    32221 ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc
    32281 gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt
    32341 aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaaa acgtaaaatc
    32401 tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa acgaggggtt
    32461 ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgcagagcgg
    32521 cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat
    32581 tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca
    32641 tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt
    32701 ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatccaga
    32761 ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca
    32821 tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc ttcgcagaag
    32881 ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc
    32941 gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct
    33001 tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct
    33061 tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa
    33121 ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta
    33181 caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc gtttacttta
    33241 tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga
    33301 ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcgaat
    33361 gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa
    33421 gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt
    33481 acccagaaaa tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc
    33541 accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg
    33601 gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact
    33661 ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc
    33721 gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct
    33781 ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca
    33841 aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc
    33901 caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact ggatgtggtg
    33961 ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc
    34021 ccgttggctt ttggcgtgca ggatggagcc agctatgccg gtgcaattct gaaagatgag
    34081 ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa
    34141 cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct taagattatg
    34201 ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg ccatgcgcgc
    34261 ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat cctgcgccag
    34321 caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct ttcgcggcat
    34381 aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca acacgacaat
    34441 gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt gcagtaccgt
    34501 gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg ttcgttgcag
    34561 ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat ggcatttgta
    34621 caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg tattccgtta
    34681 gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc aaaaagaaac
    34741 cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta tgccggatgc
    34801 ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg caatcatctt
    34861 gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag tgattaacgc
    34921 ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc ccagtcttca
    34981 tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca catgaattcg
    35041 actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc
    35101 atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat cagataatat
    35161 ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc ttctgcctta
    35221 tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag atcctggctg
    35281 tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt gccgtcaatc
    35341 ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt caggaagcga
    35401 atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg
    35461 aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt ggtaaacgga
    35521 taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga tttacccgcg
    35581 tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag cagctcacgc
    35641 ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga cgatttgaaa
    35701 cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg accgtgttta
    35761 gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc taccggcact
    35821 ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc gttctgaccg
    35881 cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg
    35941 gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc gcctttcgga
    36001 atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc aacgaccaga
    36061 atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc tgcgcggggt
    36121 tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc aatggcggaa
    36181 tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt taacatcggt
    36241 ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt
    36301 ggggtgagcg tgatgatcgc gctcacctga gctgcctgcc agcgagccat cgcttccgcc
    36361 aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc gagctgccag
    36421 tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat tgtacataaa
    36481 gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac cttttgcgcc
    36541 acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa
    36601 tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac cagaccgctc
    36661 aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag gataaatacg
    36721 ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc ggtcggactc
    36781 aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc ggtcgccact
    36841 gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa cctcttcttc
    36901 accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa cacgattgtc
    36961 ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc ccagcaaatt
    37021 gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc gtattccctg
    37081 caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa
    37141 aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa aacttattca
    37201 gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt cactttaccg
    37261 tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat
    37321 ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc accgaaacgc
    37381 ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc gccagccttt
    37441 ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta attttcacat
    37501 cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga cgaaacttaa
    37561 cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt acgccgccat
    37621 caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc acttcagcga
    37681 actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc
    37741 ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac tccgcttccg
    37801 cgcacacctt ttcaatgatt caaagtgcgc tataaatatt cacaataggg cgcgaatatt
    37861 acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa aaaacacaac
    37921 ttgtacggta acgtttatct gtgccatttt ttcagtacaa ttaccctata ttcctaacca
    37981 taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc
    38041 gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg ttaatgcggc
    38101 aatccttgag cagcttaatt ccgacgtcca actgatcaat cagttaggct attacatcgt
    38161 cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac gagctgttgg
    38221 ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc acacggcgac
    38281 tctgctacac gacgacgttg tggatgaatc agatatgcgc aggggtaaag ctaccgccaa
    38341 cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt
    38401 ccagatgatg accagcctcg gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa
    38461 cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg acatcactga
    38521 agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca
    38581 gtgttccggg attctggctg gctgtacgcc ggaggaggag aaaggcctgc aggattatgg
    38641 gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca atgccgatgg
    38701 cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct
    38761 gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta ccgccatcga
    38821 acaaggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg cttgtggatc
    38881 tcttgaatgg acgcgtcagc gcgccgagga agaagcagac aaagccatcg cagcgttaca
    38941 ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca
    39001 acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt
    39061 ccataaaaac actaattcag cgctataaca cctgaaaaaa agggcgatga atctttatat
    39121 attctgaata ttcacactct ttacaggaac tttttagcgc aataggccat caggagtata
    39181 gtgatgcccg acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag
    39241 atggaaagta atttcattga ctggcatccc gctgacatca ttgcaggttt gcgcaagaaa
    39301 ggaacgtcaa tggcggcgga atctcgcaga aatggtttga gttcctcaac gctggcaaac
    39361 gcattatcgc gcccatggcc gaaaggagag atgattattg cgaaagccct gggaactgac
    39421 ccctgggtta tctggccatc acgctaccat gatccgcaga cccatgagtt tatcgacaga
    39481 acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc
    39541 gaacggggct gccagctctc agacgattac tcgcctttca cacgctcaat atttgcacct
    39601 aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata aatacgatca
    39661 accaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgcaga
    39721 tcggttgcca taacctgtgc accagaaagt ttttcaacac catgacaaat aacggtattg
    39781 ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg gcacatgcat aaagcggttt
    39841 tcaaagaccg tttcggtgat gaacccggtc ccttcagcca ccaggttcaa cagcgtgaac
    39901 tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc
    39961 ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat gtccgctcca
    40021 gcatcacgca gtttcgccag cacggcgtcc agagtatctg gctgcgcgtt acggcagata
    40081 attttgccac gagagatcgc cgccgccacc aggaaagtac cggtttcgat acgatccggc
    40141 agcacgcgat agacaccgcc gcctaaacgt tccacacctt cgatgacgat acgatcggtg
    40201 ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc
    40261 ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg ccagggttgc agcacacatg
    40321 atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcagacga
    40381 ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg
    40441 aggccagaaa tgtgtagatc aaccggacga gcaccgatcg tacaaccgcc aggtagtgaa
    40501 acttgcccct gaccaaagcg cgctaccagc ggccccagcg cccagataga agcacgcatg
    40561 gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca
    40621 gaaccattac gttctacttt cgcacccagc tggcttagca gtttcattga tgtatcgacg
    40681 tctttcagtt tcgggacgtt ctggatctct accggttctt ccgccagtag tgcggcaaaa
    40741 agaataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt
    40801 ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct
    40861 accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca
    40921 ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc
    40981 gggccataga ccgtctgctg ttttttaacc cgactcatac cgtcaaacaa ctcacccacg
    41041 gcaataacct gaaagtgact gccatcgccg gaaacgtgga cttcctggag ggagagagcg
    41101 ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa
    41161 accagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga
    41221 taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc
    41281 cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgcgagg
    41341 gtatacactt tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt tttcgccaga
    41401 tcgataagat ggagcagcag tgccagtccc cccgtatcca cgcgggacac acggctaaga
    41461 tcgatgcagg taatcccctt caccgcttcc tcacgcattt cccaaagcgg tagcagaacg
    41521 tcctgatcca gctctccgga taacgccagc gtgtcacccg tctgcatcca gctcagtgac
    41581 tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgcg
    41641 cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg
    41701 tgatcatact gacgccttca gcaatcatgt cgtaagcttg ccagtttccc gtctgggagt
    41761 ttttacgcca ctggaagtcc agacgcaccg gcggacggcc attcgggtca ataatggtaa
    41821 cgcgaatagg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc tgataggttt
    41881 gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg
    41941 caaagtaggc ttcacgttga gcaggggtcg cactcttgta atactggccc agcaccagcg
    42001 caccggcgta tttcacctgt acgtatggca gcagttcctg atcaacaatg gtacgcagat
    42061 aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg
    42121 ccgcctcgtc catcagctta taaggattgg tctggtctgc cgcggttgcc gcactcagag
    42181 gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa
    42241 attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagctggcg
    42301 catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat
    42361 cttccagcac catcgcagac ttggtgtcct gaattgtatc gccatccttc aggatagcag
    42421 tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttct cccagcaggc
    42481 cggaagtacg aatgctcagc gaactggtat caggaatgtg gttataacgt tgttcaattt
    42541 ccagcgttac gcgcggcaga taggttttcg ggtccagcgt aatatccgcc acccggccca
    42601 caacaacacc accaatgctg accggagagc gcgctttcag gccgccaatg ttatcgaacg
    42661 tcgcataaag cgtgtaggtc gattcagtac gtatggacgt cacgttcgcc gccttcaggc
    42721 aaacaaacag cgccgccagc agtgctgcta ataaaaagat acccacccaa atttcatttt
    42781 ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc
    42841 agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac
    42901 gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc
    42961 acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc
    43021 gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagcga tccgccccag
    43081 atccccacgg cgacgaagat aaccgtcaac agtggtaatg aaataacccc agcccagaaa
    43141 cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc
    43201 tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg aaccagcacg cccggcaaac
    43261 aacagcgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc
    43321 agactggttt ccgcgctata agtggtcaga accagataac cttgcagccc caacaccatt
    43381 ccgatgaaca cgccagaaac tacaataatc agcatcgaca ggacgccgac attatagagc
    43441 tggcgcacca gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat
    43501 aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg tccgagcgac
    43561 gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc
    43621 gccggcagga tagcggaacg gaacaggccc gtcagctatc ccgtccagaa actgacgtac
    43681 gcgcggatca ggattcgtct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc
    43741 cgccaggatc caggcgtgat ccgcaatact taacacttcc ggcacatcgt gagaaaccac
    43801 cacacaagtc acgcccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat
    43861 ggtgatggga tcttgcccaa caaaaggttc atcaaacatg atgagatccg gctccagcgc
    43921 aatcgcacgc gccagcgctg cacgccgcgc catcccaccg gaaagttcag aaggcattag
    43981 tttagccgct ccacgcagcc ccacggcctc cagcttcatc atcaccgtac tatgcaacaa
    44041 tggcgcggga agttgggtat gttcgcgcag tggataggcg acgttgtcaa atacgttcat
    44101 atcagtgaac aacgccccgg actgaaataa catgctcatc cgtttacgca ctgtatacag
    44161 gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg
    44221 tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat
    44281 gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt
    44341 gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat
    44401 tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac
    44461 atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag
    44521 ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc
    44581 gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt
    44641 tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa
    44701 cctttggcat cccgccgctg atcatcggca tgacggtggt cagtattggt acatcgttac
    44761 cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta
    44821 cagccctcgg ctcaaacatt atcaatatat tgctgatcct cggtctggct gcgctggttc
    44881 gtccttttac cgtccattcc gatgttctgc gccgtgaatt acccttaatg ttgttggtca
    44941 gcgtggtggc cggttccgta ctctatgacg gacaacttag tcgaagcgat ggtatctttc
    45001 tcctctttct ggctgtgcta tggctgctgt tcattgttaa acttgcacgt caggggactg
    45061 acagcctgac cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt
    45121 ttttatggct cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata
    45181 acgccacggt gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa
    45241 ttgctatcgg aaccagtctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg
    45301 aaaacgacat tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt
    45361 tgggtttacc cgcgctgata acgccaggag agattgatcc actggcgtac agtcgtgact
    45421 acagcgtgat gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc
    45481 aaccgggccg tggtgtaggg gtattattaa ctggcggatt tatcatatgg ctggcgatgt
    45541 tgtactggtt atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag
    45601 ttacaaccgg gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa
    45661 tgcctggcgg agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg
    45721 ttctggtgta aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc
    45781 aaaatggccg caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctagtgaa
    45841 gccgcgcatg gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac
    45901 tcaggtgaat ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg
    45961 ttaatctgca tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg
    46021 tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc
    46081 gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact
    46141 gctgaagatt ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc
    46201 gtaaacgata ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg
    46261 cgtgacgcat tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac
    46321 aatatgatga ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc
    46381 gtggatgttc gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc
    46441 cctggcattc tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg
    46501 atggttgccg atggcgacca tttactcggt gtgttacata tgcatgattt actgcgtgca
    46561 ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga
    46621 cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc agagaacatt cgtctgctga
    46681 tcctcgacgt tgatggcgta ctgtcagatg gcctgattta tatgggcaat aacggcgaag
    46741 agctgaaagc gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata
    46801 ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat
    46861 tggggatcac tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatttgc
    46921 tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt tggcgatgat ctcatcgact
    46981 gcccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcccat ccactgttga
    47041 tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt
    47101 gcgacttatt actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag
    47161 taaagccaga cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat
    47221 taatatggcc gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta
    47281 taaaagcgag catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt
    47341 gattgctcaa cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt
    47401 acttaccacg tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa
    47461 gctgaccaat gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc
    47521 agactctcaa cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga
    47581 tgttacctct gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa
    47641 aatgcgcggc aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta
    47701 tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg
    47761 ccagcattcc ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg
    47821 accagcaatc tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca
    47881 cccagggcac catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac
    47941 aaggtaaaga agtgattgac ggatacggta aaccggcaac gttctaccag atgcaggaca
    48001 acggtaaacc cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg
    48061 tcattctgac gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga
    48121 tcacttacct ggtgaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa
    48181 ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac
    48241 agaagaaggg taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct
    48301 ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg
    48361 gtctgctggg gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg
    48421 tgccgcgcga tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc
    48481 atgcacgcgc gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc
    48541 tcagcgttta cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac
    48601 aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca
    48661 gcatggggca gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg
    48721 ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg
    48781 ttatcgatat taaacgcatc attgaacacc tgcgcgacag cggcctgggc gtgctgatca
    48841 ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg
    48901 ggcatttgat cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg
    48961 tataccttgg ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga
    49021 gagtacgatt ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat
    49081 gacgccacag ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca
    49141 ggagctacag caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga
    49201 aatcgacacc cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca
    49261 aaaagagatg ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg
    49321 tacaccatcc ggcaccagcg gtgactacat tgacgacgag ctgccggtct accagggcga
    49381 aacgacgcag accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga
    49441 cactgaccgc gctattgcta cctctatcgt cgatgccgtt gatgaaaccg gttatctgac
    49501 tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt
    49561 tgaagccgtc cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct
    49621 gcgtgactgc ctgctaatcc aactctccca attcgataag accacgccgt ggctggaaga
    49681 ggccagattg atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt
    49741 aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc
    49801 gctcgatccg cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga
    49861 tgtgctggtg cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc
    49921 gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga
    49981 cagccagttt atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag
    50041 ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt
    50101 tgagcaaggt gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga
    50161 aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg
    50221 catttttgaa ctgaagtatt tcttttccag tcacgtcaat accgagggcg gcggcgaagc
    50281 ttcctccacg gcgattcgtg cactggtgaa gaaattaatc gcggcggaaa acccagcgaa
    50341 accgttgagc gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg
    50401 ccgcactgtt gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca
    50461 gctcgtttga cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg
    50521 tcgagatcac cgaggcactg cgcgaatttg ttacagctaa atttgccaaa cttgagcaat
    50581 attttgaccg aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct
    50641 cagatgcaac actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca
    50701 tgtacgctgc cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag
    50761 ataaactgaa acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc
    50821 ggcacaacgg gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca
    50881 ggttcttagg tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta
    50941 acagggaatg tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca
    51001 tcagcgagct ggcggcgaaa caacttagcc taccgcctca ggtggttttt gaagctatcc
    51061 tgacgcggga aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca
    51121 aacttgaaga agatactctg cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag
    51181 ctttcgatgc catcgacaac caaccggtgg acttactttt tgccctgctg gtgccggcag
    51241 accaaactaa aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa
    51301 ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg
    51361 ataccgaagg tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg
    51421 aggagaaaca gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg
    51481 tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt
    51541 tgttacccga tctggctcga actctggccg atcgcgagat ttctgccgcc gtcagcattg
    51601 atgttcgtaa tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg
    51661 acgctttctc accgcaacta ctgttcctgg atgccgaccg taatacctta attcgtcgtt
    51721 acagtgacac gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta
    51781 tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata
    51841 cctcagaaat gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac
    51901 gtgaacgcga actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg
    51961 atgcagatta cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc
    52021 gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac
    52081 acaattttat ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca
    52141 acaaccgtag ctacctgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt
    52201 atattgcaga gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc
    52261 atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa
    52321 gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc
    52381 tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa gccaacagcg ttattgcgct
    52441 gctgatgttg gattctgcca aaggacggca gattgaagtt gaagcgaccg gtccacagga
    52501 agaggaagca ctggccgccg ttatcgccct ctttaattct ggttttgatg aagattaatc
    52561 ttcatcactt tgacatacaa ctcccttcaa gctcccccga caataagaaa atcacgtact
    52621 gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg cttttcactt tcagaagaac
    52681 cttaagaaaa ccttaagagg cattgtttag gttttgttta agttaatcga ccatactgga
    52741 gatcgtcaga aaatatttcc aggagatggc atgattcgct tatctgaaca aagtcccctg
    52801 ggcaccggaa gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag
    52861 attgtctacc atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac
    52921 gcgcatcttg gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt
    52981 gagacggatt gcggaaccgg ttatgtctac gatgtgattg ccgattttga cggcaagcct
    53041 tccattacgc tgaccgaatt tgctgaacaa tgccgttacg aagaagacat tgcacaactg
    53101 cgccagctac tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg
    53161 ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc
    53221 tgcgacaaca ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt
    53281 ctgcgtaagc aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc
    53341 ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct
    53401 taatccagct ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa
    53461 atccacgcct gacggctacg cacgtagccg tcaggcgtgg agactttgaa acgaagtgga
    53521 ttaggtaata cagctgccag taatgcagct tccgaccggg taagtttgct cgcgggtttg
    53581 tggaaataac gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata
    53641 ttcaggtaaa ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat
    53701 ccagcttcca gcccttttcg tacccagcta cgcccatccc ataaaaagag atttttggcc
    53761 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    53821 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    53881 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    53941 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    54001 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    54061 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    54121 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    54181 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    54241 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agtacttctg
    54301 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    54361 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    54421 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    54481 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    54541 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    54601 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    54661 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    54721 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    54781 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    54841 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    54901 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    54961 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    55021 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    55081 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    55141 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    55201 tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag
    55261 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    55321 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    55381 gtcgtcaccg tactcttctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    55441 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    55501 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    55561 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    55621 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    55681 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    55741 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    55801 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    55861 ttgccctgtt cgctggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    55921 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    55981 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    56041 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    56101 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    56161 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    56221 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    56281 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    56341 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    56401 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    56461 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    56521 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    56581 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    56641 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    56701 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt cagatgaacc
    56761 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    56821 ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcat caaggaagga acgtaagaat
    56881 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    56941 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    57001 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    57061 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    57121 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    57181 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    57241 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    57301 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    57361 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    57421 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    57481 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    57541 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    57601 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    57661 caataccgtt caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    57721 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    57781 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    57841 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    57901 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    57961 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    58021 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    58081 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    58141 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    58201 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    58261 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    58321 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    58381 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctaga
    58441 tcaatcgtcc cgccctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    58501 gcataatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    58561 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    58621 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    58681 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagcc
    58741 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    58801 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    58861 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    58921 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    58981 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    59041 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    59101 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    59161 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    59221 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    59281 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    59341 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    59401 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    59461 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    59521 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    59581 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    59641 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    59701 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    59761 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    59821 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    59881 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    59941 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    60001 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    60061 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    60121 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    60181 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    60241 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    60301 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    60361 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    60421 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    60481 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    60541 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    60601 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    60661 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    60721 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    60781 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    60841 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    60901 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    60961 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    61021 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    61081 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    61141 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    61201 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    61261 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    61321 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    61381 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    61441 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    61501 aagctggtta atgagcgtcc gacaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    61561 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    61621 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    61681 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    61741 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    61801 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    61861 gaaggcggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    61921 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    61981 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    62041 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    62101 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    62161 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    62221 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    62281 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    62341 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    62401 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    62461 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    62521 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    62581 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    62641 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    62701 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    62761 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    62821 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    62881 gtgtggaacg cgggcggcgt ggaactatac ctgaccgggg atgccaacga ctatgtcggt
    62941 aaaggcatgg cgggcggctt aatcgccatt catcctccgg ttggttccgc cttccgcagc
    63001 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    63061 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    63121 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    63181 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    63241 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    63301 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    63361 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    63421 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    63481 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    63541 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    63601 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    63661 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    63721 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    63781 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    63841 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    63901 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    63961 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    64021 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    64081 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    64141 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    64201 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    64261 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    64321 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    64381 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    64441 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    64501 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    64561 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    64621 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    64681 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    64741 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    64801 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    64861 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    64921 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    64981 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    65041 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    65101 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    65161 atccacgacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    65221 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    65281 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    65341 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    65401 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    65461 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    65521 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    65581 gtatattcat gcaaataaac gaagg
//
LOCUS       DADLTI010000005        56574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLTI010000005 DADLTI010000000
VERSION     DADLTI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.005"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JN755_000333"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JN755_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7379248.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JN755_000334"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JN755_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7379249.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /locus_tag="JN755_000335"
     CDS             1339..2994
                     /locus_tag="JN755_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7379250.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="JN755_000336"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="JN755_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7379251.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="JN755_000337"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="JN755_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7379252.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="JN755_000338"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="JN755_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7379253.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="JN755_000339"
     CDS             complement(6523..7287)
                     /locus_tag="JN755_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7379254.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JN755_000340"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JN755_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7379255.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="JN755_000341"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="JN755_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="JN755_000342"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="JN755_000342"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7379256.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="JN755_000343"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="JN755_000343"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7379257.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="JN755_000344"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="JN755_000344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7379258.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="JN755_000345"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="JN755_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7379259.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="JN755_000346"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="JN755_000346"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7379260.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JN755_000347"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JN755_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7379261.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="JN755_000348"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="JN755_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7379262.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="JN755_000349"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="JN755_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7379263.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="JN755_000350"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="JN755_000350"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7379264.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="JN755_000351"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="JN755_000351"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7379265.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="JN755_000352"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="JN755_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7379266.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="JN755_000353"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="JN755_000353"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7379267.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JN755_000354"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JN755_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7379268.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JN755_000355"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JN755_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY7379269.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JN755_000356"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JN755_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7379270.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="JN755_000357"
     CDS             complement(29898..31292)
                     /locus_tag="JN755_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379271.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JN755_000358"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JN755_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7379272.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="JN755_000359"
     CDS             complement(31635..32921)
                     /locus_tag="JN755_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7379273.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="JN755_000360"
     CDS             complement(32972..33913)
                     /locus_tag="JN755_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7379274.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="JN755_000361"
     CDS             complement(33928..34698)
                     /locus_tag="JN755_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7379275.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="JN755_000362"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="JN755_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY7379276.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="JN755_000363"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="JN755_000363"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7379277.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="JN755_000364"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="JN755_000364"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY7379278.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="JN755_000365"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="JN755_000365"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7379279.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="JN755_000366"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="JN755_000366"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7379280.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="JN755_000367"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="JN755_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7379281.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JN755_000368"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JN755_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7379282.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43062..46283)
                     /gene="carB"
                     /locus_tag="JN755_000369"
     CDS             complement(43062..46283)
                     /gene="carB"
                     /locus_tag="JN755_000369"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7379283.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46301..47449)
                     /gene="carA"
                     /locus_tag="JN755_000370"
     CDS             complement(46301..47449)
                     /gene="carA"
                     /locus_tag="JN755_000370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7379284.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47905..48726)
                     /gene="dapB"
                     /locus_tag="JN755_000371"
     CDS             complement(47905..48726)
                     /gene="dapB"
                     /locus_tag="JN755_000371"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7379285.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48893..49807)
                     /gene="rihC"
                     /locus_tag="JN755_000372"
     CDS             complement(48893..49807)
                     /gene="rihC"
                     /locus_tag="JN755_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7379286.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49873..50823)
                     /gene="ispH"
                     /locus_tag="JN755_000373"
                     /gene_synonym="lytB"
     CDS             complement(49873..50823)
                     /gene="ispH"
                     /locus_tag="JN755_000373"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7379287.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50825..51274)
                     /gene="fkpB"
                     /locus_tag="JN755_000374"
     CDS             complement(50825..51274)
                     /gene="fkpB"
                     /locus_tag="JN755_000374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7379288.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51361..51855)
                     /gene="lspA"
                     /locus_tag="JN755_000375"
     CDS             complement(51361..51855)
                     /gene="lspA"
                     /locus_tag="JN755_000375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7379289.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51855..54671)
                     /gene="ileS"
                     /locus_tag="JN755_000376"
     CDS             complement(51855..54671)
                     /gene="ileS"
                     /locus_tag="JN755_000376"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7379290.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54714..55655)
                     /gene="ribF"
                     /locus_tag="JN755_000377"
     CDS             complement(54714..55655)
                     /gene="ribF"
                     /locus_tag="JN755_000377"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7379291.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55663..55881)
                     /locus_tag="JN755_000378"
     CDS             complement(55663..55881)
                     /locus_tag="JN755_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7379292.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55984..56247
                     /gene="rpsT"
                     /locus_tag="JN755_000379"
     CDS             55984..56247
                     /gene="rpsT"
                     /locus_tag="JN755_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7379293.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13541 a        15497 c        14684 g        12852 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccggcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg gatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc cccctggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc
    43021 gatcaaatta gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc
    43081 atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg
    43141 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
    43201 acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg
    43261 ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc
    43321 ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
    43381 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
    43441 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg
    43501 aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc
    43561 cctaacagcg ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag
    43621 tagtacggcg gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
    43681 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta
    43741 cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc
    43801 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
    43861 acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag
    43921 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
    43981 cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc
    44041 gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
    44101 acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca
    44161 atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt
    44221 ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata
    44281 gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc
    44341 agcggggttt gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
    44401 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
    44461 gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc
    44521 gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga
    44581 ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
    44641 tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg
    44701 cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt
    44761 acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
    44821 aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt
    44881 tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca
    44941 gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc
    45001 agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag
    45061 ccagttgcgc ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    45121 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg
    45181 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    45241 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc
    45301 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    45361 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    45421 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
    45481 gaggcgttac gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg
    45541 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    45601 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    45661 tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca
    45721 aactcttcac ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    45781 ataatgcacg ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg
    45841 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    45901 tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc
    45961 aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt
    46021 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc
    46081 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    46141 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    46201 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    46261 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    46321 actgctcaat taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc
    46381 tggcttcagg gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta
    46441 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    46501 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    46561 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    46621 gcgccagcag ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact
    46681 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    46741 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    46801 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    46861 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    46921 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    46981 ccatgccgtt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt
    47041 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    47101 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    47161 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
    47221 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    47281 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    47341 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    47401 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
    47461 tagaatattc actcacttta tttgcatatt aattcacagt gatgacgtaa atcaatgcaa
    47521 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
    47581 tcaacttaat ggcaggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
    47641 aagcgacaaa aacatcatct aacctgttct ggaaaacgct tgcgcagcaa gaagattgca
    47701 tgaaataaaa acgagcagga aaaagtggac caaatggtca aaatttacat taaaacaaca
    47761 aaaatagaag acgaaacatg taatttttgg cagttataga aaatttaaaa gcataacacc
    47821 aaaaataaaa gggctaaaaa ttaaagccct ttataattaa taaattaaat gtgttacttt
    47881 tgcatcataa taaacatttt gtggttacaa attattgaga tcaagcacat ctcgcatatc
    47941 aaaaagacca ctttccttac cactcaacca caaagccgat cttaccgcac cgttagcaaa
    48001 tgtcatgcgg ctggacgcct tatgggtgat ctccagacgc tcgccaatat cggcaaacat
    48061 cgcggtatgt tcgccaacga tgtcacctgc acgcacggtg gcaaaaccaa tggtaccagg
    48121 cacacgttca ccggtatggc cttcacgact gtagaccgcg caatctttca gatctttatc
    48181 aagggcgtgg gcgatcgcct ctcccattgc cagtgcggtg cctgacggcg catcaacttt
    48241 atgtctatga tgtgcttcaa taatttcgat atcggtgtag tcacccatca ctttggctgc
    48301 tttctccagc agcttaagca tgacgttaac gccaacgcta aaattggcgg caaagacaat
    48361 cgcaatatcg gcggcggcgt cacgaattgc ctgtttaccg gcttcgtcaa accccgtagt
    48421 gccgatcacc atccctttgc catgctgccg acaaaaagcg agatggttca gcgtaccttc
    48481 cggacgggta aaatcgataa acacatcaaa atcatctttt atcgcatcaa gactgctttg
    48541 cacggtaacg cctgttttcc ctgcaccagc cagctcaccg gcgtcgctgc ccagtaaaga
    48601 agatccttcg cgctccagcg ccgcgcccag ctgcacgcct tctaatgcca gtgccgcctg
    48661 aatcaactgg cggcccatac gccccccggc tcccgcgatg gcaacgcgga tgtttgcatc
    48721 atgcatagct attctctttt gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc
    48781 gctacgccgc cagccaaaaa cgctgataat tagaaattaa tgataaacag ggagggatat
    48841 cagtgaaagg catgagtcga tgaatgactg catgccgata acatgtgaca ggttacggca
    48901 ccagagccag cacctcagcc acccactgtt ggaagccttt tacattcaga tccaatgcca
    48961 cctgtacatt ggctggtttg cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg
    49021 taaattcgcc ttgagtttct accgcgacaa aacacggctt gagggtgaat aagtccgggc
    49081 gcaccagcca ggcgatggcg cagagatcgt gcattcgcag accgctttgc atactgccgc
    49141 tacggtagtg gctaaacagg gcgtggagca tttttccggt gcgattcaac tccggtaaag
    49201 tagcgagata gtcaggggtt aatattgcct gattggtgac atccagaccg cacatgacaa
    49261 tttcgatgcc gctacggaag acacaggcag ccgcttctgg atcggcagcg atattaaact
    49321 cggcatttgg tgtacagttg ccgcgaccag cagaaccgcc catgatcacc agacgacgaa
    49381 tatacggttt acattccggg cattgtaaaa gtaacagcgc aatattggtt aacgggccga
    49441 tggccaccag ggtaacgggc tctggtgcac gcatcagggc atcccgaatc gccagaaacg
    49501 ccggtatccc gagtggcttt cggttgtgct caacaaagtc gtagccagcc attcccgatt
    49561 cgccgtgcac agatgctgca tcacgcggtg cgcgtaccag cggcacagcg gctccctggg
    49621 cgagcggaat ctccgcattc cagaaatgca gcagttgcag ggcattgcgg gtagttttct
    49681 caaccgagac attacccgcg acggtggtca tcagttgcag gtcgagttcg ggggcaaaaa
    49741 tcgcggcggc aatggcgacg gcatcgtcaa tgccggggtc agtatcgagg aagataggta
    49801 aacgcatgtt ttcttcataa aaaatgccgg taacaagacc ggcattttcg cataacttag
    49861 gctgctaatg acttaatcga cttcacgaat atcgacacgc agctctttcg gcacttcgaa
    49921 aacaatattt tcttcacggc cttccagcgg aatggcttca ccaccaccca actgctgcaa
    49981 acgtgccacc acattctgca ccagaatatc cggagccgat gcgcccgcag tcacgccgac
    50041 gcatttaacc tctttcaccc actcttcctg gatatctttc gcatcgtcaa tcaaaaacgc
    50101 gtgtttgccc atacgctggg ccagctccgc cagacggttg gagttggagg agtttttcga
    50161 accgaccacc aacacaactt ccgcctgttc tgccagggcg cgtaccgctt cctgacggtt
    50221 agtcgtggcg tagcagatgt catctttgcg cggaccgaca attttcggga agcgtttacg
    50281 cagcgcgtcg atcacatcag acgtatcatc caccgacagc gtggtttggg tcataaagga
    50341 gagcttctct tcgtttttga ccgtcagttt ccacacatcg tctggcgatt cgaccagata
    50401 cattccccct tcggggttgc tgtactgccc catcgtccct tccacttccg ggtgcccggc
    50461 gtggccgatg agaatagatt cttcgccacg gcgactggcg cgggcaactt ccatatgcac
    50521 tttggtcacc agcggacagg tggcatcaaa caccgtcaaa tcgcggcttt tcgcttcgtt
    50581 acgaaccgcc tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt ccggcacttc
    50641 gctaatctgc tcaataaaga tagccccacg ctcgcgcaag ctatcgacca cgtagcggtt
    50701 atgcaccact tcgtgacgga catatatcgg tgcgccgtaa atggccagcg cgttttcaac
    50761 aatgctgata gcgcggtcta ccccggcaca aaaaccacgc gggttggcca acaggatctg
    50821 catgttacgc ctccagtgcc ggatcgattt ccagcacttc aatatcaaaa tgaacggtct
    50881 gcccggccag cggatggttg aaatcaacgg taatggagtc gccgttaatt tcgcggatca
    50941 caccaggcat ctcactgcca tccattgcgg taaaaagcat gattgcgcca atttctggct
    51001 cgcctgcatc cataaattca cggcgggaga agtactgaat caggtccggt gacggcacgc
    51061 caaacgccgc atctggctcc agcgagaagg tggttttgtc gcccactttc agccccagca
    51121 ggtgttgctc cagcccttca gaaagagaag catcgcccag gcggaacagc gccggtttac
    51181 cgttgttgcg agtagactcg gcggtggtgc catcgtcgag ttttagcgtg aagtgcacca
    51241 ggacggcgct attgctctgt acagattcag acatgaaggt ttgctcttat ttttgaggat
    51301 ttttgccgga tgcggcctac gatttcgtag caatctgtag gccgcatccg gcagggttta
    51361 ttattgtttt ttcgctttgg aaggtaaaaa cccttccagc acgatcagtg ccgcaccgac
    51421 acaaatggca gtatcggcaa ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc
    51481 gatcatatcg acaacgaagc cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc
    51541 aataatcagc gcgtaagcga tattgtttag cttctgcgtg gccttcgagc gatacatcat
    51601 cactgccagg agcacgctaa taccaatcgc aataccggca aagaaccaac gctgccagcc
    51661 gccgctatcg gcaaggaaac taaacgccgc gccatagtta cgcgcataat gcagattaag
    51721 cgacgggaac agcgggaccg tatcccccag agcaaagttc tggaggatca ggtatttgct
    51781 gcccagatcg ataatcagca cgactaccac cagccacagc cagcgtagcc ctgttgaaca
    51841 gatcgattga ctcatcaggc aaacttacgt ttttcaccgt caccggcgac gttgctgaca
    51901 cagcggccgc agatttctgc gtgttccgcc accttgccga catcctgggt gtagtgccag
    51961 cagcgtgggc acttctcacc ttcggcttta ctcaacgcga ctttcagccc tttgagtact
    52021 tcgctctgct gagcatcagc aggtgcgtcg ttatagtctg caacggtagc gccggaggtc
    52081 aacaggacaa atcgtaattc atcgcccagc gcggtcagtt tcgccgccag ttccggttct
    52141 gcatacaagg ttactgccgc ttccagcgag ccacccactt tcttgtcggc acgcgcttgc
    52201 tcaatgactt tgttcacttc gccacgcact ttcaacagct cgtcccagaa cgcatcgttc
    52261 attgcttcac tgtctgccag accaaacagg ccttcgtacc actcaccggt gaagacgtat
    52321 ttttcacgtt cgcccggcag atagccccac acttcatcag cggtgaagga gaggattggt
    52381 gccatccagc gcaccagcgc ttcagcgatg tgatacagcg cagtctggca gctacgacgc
    52441 gccacgctat ccgctttggc ggtgtactga cggtctttga tgatgtcgag gtagaaggaa
    52501 cccatctcaa cggagcagaa gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct
    52561 tcgtacgcct tgaggatgtc ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc
    52621 agtaccacca tctcttccgg tttcaccata tcttttgctg gatcaaaacc attcaggttt
    52681 gccagcagga agcgcgcggt gttacggata cgacgatagc tatcggcagc acgtttcagg
    52741 atctcgtcag aaacggccat ttcaccggtg tagtcggttg atgccaccca cagacgcaga
    52801 atatccgcgc ccagtttgtt catcacatcc tgcggcgaaa cggtgttgcc gatggattta
    52861 gacatcttgc gaccctgacc atccacggta aagccgtggg tcagtacctg acgatacggc
    52921 gctttgcctt tcatcgcggt ggagatcatt agggaagaca tgaaccaacc acggtgctgg
    52981 tcagaacctt ccagatacat gtccgctgcg tgaccggcaa attccggacg cacgtcaaca
    53041 acagaagagt gggtagatcc ggagtcaaac catacgtcga gagtatccgg cactttcaca
    53101 tactggtcag cttcgtcgcc gaggatctct ttcgcatcga gatcccacca cgcctggatg
    53161 ccgtcaactt caacgcgttt agccacttct tccatcagtt cgagggtacg cggatgcagc
    53221 tcttccgtgt ctttgtgcac gaacagtgac atcggtacgc cccaggtgcg ctgacgggag
    53281 atacaccagt caggacggtt agcaaccatc gactcgatac gcgcctggcc ccagtccggg
    53341 atccactgca cgcctttgat ctctttcagt gactgcgcac gcagaccttt ctgatccatg
    53401 ctgacgaacc actgcggtgt cgcgcggaag atgatcggcg ttttgtgacg ccagcagcac
    53461 ggatagctgt gctgcatttt ctcaacgtgc agcagcgcgc ctttttcctg cagcagcgca
    53521 acgacgatgt cgttcgcttt gaagacgttc acgccatcca gcgtcggata agtgcccggc
    53581 agataagtgc cgtccgggcc aaccgggtta gcggtttcca ggccgtattt ctgaccgatc
    53641 acatagtcgt ccgggccgtg gccaggcgcg gtgtgaacgg caccggtacc ggcatccagg
    53701 gtaacgtgat cgccgaggat tgccggaacg tcgaagccca taaacggatg ggtaaagcgc
    53761 agcagctcaa gctctgcacc ttttaccgtg ccgagaatgg tgtaatcggt cacgccgata
    53821 cgctgcatta cgctttcaac cagatctttc gccagaatcg cggcctgacc gtcgatctgc
    53881 accagcgcat agtcgaaatc aggtgcaata gagattgcgc ggttcgcagg caaagtccac
    53941 ggcgtagtgg tccagatcac cagcgagatt gggccgttaa cgttgcttac gccaaatttc
    54001 gctttcagcg catcctgatc gaccgcctgg aaagcgacgt caatggacgg agaagttttg
    54061 tcgtaatact caacttccgc ttctgccagt gcagaacggc agtctacgca ccagtgcacc
    54121 ggcttcgcgc ctttgtgcag gtgaccattg ccgatgattt tgcccagcgc gcggatgatg
    54181 ttggcttcag ttttgaagtc catagtcaga tacgggtgcg accagtcgcc cagcacgccc
    54241 agacggataa agtctttgcg ttgaccgtca acctgggtcg ccgcgtattc gcggcacttg
    54301 gcgcggaact cggcggcggt gaatttctca cccggcttac cgtattcttg ctctactttc
    54361 agctcgatcg gcagaccgtg gcagtcccag ccaggcacat acggcgagtc ataaccggaa
    54421 agccctttgg acttcacgat aatgtctttc agaatcttgt taaccgagtg accaatatga
    54481 atgctgccat tcgcataagg agggccatca tgcagaatga aggttttttt gcctttttta
    54541 gccgcacgga tgatgccgta cagatcatca tcagtccaac gcgccagcat tcccggttcg
    54601 cgcttggcga gatcgccacg catcgggaac cctgtttccg gcaaattcag ggttgattta
    54661 tagtcactca tcagattctc ggttccgtgt ttcggtttga ttacataaca ggcttaagcc
    54721 ggttttgtta gcccaaaaaa ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc
    54781 agttcgtcca gcgacgcaaa tcgctgctca ttgcgtattt ttttacgcag cactacttgt
    54841 atatggcgac cgtaaaggtc cattgcaaca tctaacaaat gcacttccag ctgctggcga
    54901 ataccggcaa ccgttgggcg tgttccgatg tttgccacgc cgggtaacgg cttttcaccg
    54961 aggcccagca cttctaccgc ataaacccct ttcaccgggg aaacctgacg gcgtagcggt
    55021 acattcgccg tcgggaaacc tatagtgcgc cctaattcat caccgtggac tacacgcccg
    55081 gagatggcaa acgggtgccc cagtaaactt tctgccagag ccagattgtc atcagccagc
    55141 gcctgacgca cggcggtgct gctgatacgc acaccacctt cgcaaaaagt ttgcgtactg
    55201 gtgatatcga agccgtattc catgccagct ttctgtaata acaagaaatc gccttcacga
    55261 ccagcgccaa agcggaaatc atcacctacg gcaagaaatt ttacgcgcaa atgcttcacc
    55321 aaaagatcgc tgatgaaatt ttgcgcggtt aacgccgcaa aacgcctgtc gaaacgcacg
    55381 cacagcacgt aatcaacgcc acactctgca aggtaacgca gtttttcccg cagccgggtc
    55441 agtcttgccg gggctttatc ggtagcaaac agttccagtg gttgaggttc aaaaagcatc
    55501 accatcaccg gtaagttgcg cttgcgccct tcttcctgca agccctgtaa cagcgcgcga
    55561 tgaccgcgat gcacgccgtc gaaattacca atagtcagca cacacccttc ttgcggggcc
    55621 tggctgagat tatgtatgcc gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg
    55681 tccgagtata ccttgtacag cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa
    55741 gaagtggctt ttcattgcca tggcgcaaat cacgggaaga aactgaccgc ctgctgcaat
    55801 ttttatcgcg gaaaagctgt attcacaccc cgcaagctgg tagaatcctg cgccatcact
    55861 acgtaacgag tgccggcaca ttaacggcgc ttatttgcac aaatccattg acaaaagaag
    55921 gctaaaaggg catattcctc ggcctttgaa ttgtccatat agaacacatt tgggagttgg
    55981 accttggcta atatcaaatc agctaagaag cgcgccattc agtctgaaaa ggctcgtaag
    56041 cacaacgcaa gccgtcgctc tatgatgcgt actttcatca agaaagtata cgcagctatc
    56101 gaagctggcg acaaagctgc tgcacagaaa gcatttaacg aaatgcaacc gatcgtggac
    56161 cgtcaggctg ctaaaggtct gatccacaaa aacaaagctg cacgtcataa ggctaacctg
    56221 actgcacaga tcaacaaact ggcttaatcg ccaatttgct gaagctttgt gaaaaagccc
    56281 gcgcaagcgg gtttttttat gcctgctgct tttgtgatgc gtgagagaac acactgccgg
    56341 ttgccagata gtcaaaagag acatgccgtt gtactatctg aactcttcgt ggcttaagaa
    56401 tcgtagaacc atcggctgat acattctgaa caagaatatc tactcaatct ccagactccc
    56461 ccacaaagaa tatggatatt gtgatacaca ttgaggtatt aaacttaaga ccactctatt
    56521 tttgaattaa attcctatat actttaacca ttcataaata tctcctcatt agac
//
LOCUS       DADLTI010000006        52973 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLTI010000006 DADLTI010000000
VERSION     DADLTI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.006"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..326
                     /locus_tag="JN755_000380"
     CDS             <1..326
                     /locus_tag="JN755_000380"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7379294.1"
                     /translation="GLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYN
                     LGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLK
                     HANHK"
     gene            complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN755_000381"
     CDS             complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN755_000381"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7379295.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            1109..3499
                     /locus_tag="JN755_000382"
     CDS             1109..3499
                     /locus_tag="JN755_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7379296.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(3546..5095)
                     /gene="cueO"
                     /locus_tag="JN755_000383"
                     /pseudo
     CDS             complement(3546..5095)
                     /gene="cueO"
                     /locus_tag="JN755_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            5261..5608
                     /locus_tag="JN755_000384"
     CDS             5261..5608
                     /locus_tag="JN755_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7379297.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            5714..6580
                     /gene="speE"
                     /locus_tag="JN755_000385"
     CDS             5714..6580
                     /gene="speE"
                     /locus_tag="JN755_000385"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7379298.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            6596..7390
                     /gene="speD"
                     /locus_tag="JN755_000386"
     CDS             6596..7390
                     /gene="speD"
                     /locus_tag="JN755_000386"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7379299.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(7428..7790)
                     /locus_tag="JN755_000387"
     CDS             complement(7428..7790)
                     /locus_tag="JN755_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7379300.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN755_000388"
     CDS             complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN755_000388"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7379301.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            10918..12771
                     /locus_tag="JN755_000389"
     CDS             10918..12771
                     /locus_tag="JN755_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7379302.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(12842..14272)
                     /gene="lpdA"
                     /locus_tag="JN755_000390"
     CDS             complement(12842..14272)
                     /gene="lpdA"
                     /locus_tag="JN755_000390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7379303.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(14597..16489)
                     /gene="aceF"
                     /locus_tag="JN755_000391"
     CDS             complement(14597..16489)
                     /gene="aceF"
                     /locus_tag="JN755_000391"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7379304.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(16504..19167)
                     /gene="aceE"
                     /locus_tag="JN755_000392"
     CDS             complement(16504..19167)
                     /gene="aceE"
                     /locus_tag="JN755_000392"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7379305.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRAGNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19328..20092)
                     /gene="pdhR"
                     /locus_tag="JN755_000393"
     CDS             complement(19328..20092)
                     /gene="pdhR"
                     /locus_tag="JN755_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7379306.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            20633..22006
                     /gene="aroP"
                     /locus_tag="JN755_000394"
     CDS             20633..22006
                     /gene="aroP"
                     /locus_tag="JN755_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7379307.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(22049..22903)
                     /gene="ampE"
                     /locus_tag="JN755_000395"
     CDS             complement(22049..22903)
                     /gene="ampE"
                     /locus_tag="JN755_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7379308.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(22900..23451)
                     /gene="ampD"
                     /locus_tag="JN755_000396"
     CDS             complement(22900..23451)
                     /gene="ampD"
                     /locus_tag="JN755_000396"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7379309.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            23539..24432
                     /gene="nadC"
                     /locus_tag="JN755_000397"
     CDS             23539..24432
                     /gene="nadC"
                     /locus_tag="JN755_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7379310.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            24635..25075
                     /gene="ppdD"
                     /locus_tag="JN755_000398"
     CDS             24635..25075
                     /gene="ppdD"
                     /locus_tag="JN755_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7379311.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            25085..26470
                     /gene="gspE"
                     /locus_tag="JN755_000399"
     CDS             25085..26470
                     /gene="gspE"
                     /locus_tag="JN755_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7379312.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            26460..27662
                     /gene="hofC"
                     /locus_tag="JN755_000400"
     CDS             26460..27662
                     /gene="hofC"
                     /locus_tag="JN755_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7379313.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(27697..28740)
                     /gene="guaC"
                     /locus_tag="JN755_000401"
     CDS             complement(27697..28740)
                     /gene="guaC"
                     /locus_tag="JN755_000401"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7379314.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            28965..29585
                     /gene="coaE"
                     /locus_tag="JN755_000402"
     CDS             28965..29585
                     /gene="coaE"
                     /locus_tag="JN755_000402"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7379315.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            29585..30328
                     /gene="zapD"
                     /locus_tag="JN755_000403"
     CDS             29585..30328
                     /gene="zapD"
                     /locus_tag="JN755_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7379316.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            30338..30535
                     /gene="yacG"
                     /locus_tag="JN755_000404"
     CDS             30338..30535
                     /gene="yacG"
                     /locus_tag="JN755_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7379317.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(30635..31024)
                     /gene="mutT"
                     /locus_tag="JN755_000405"
     CDS             complement(30635..31024)
                     /gene="mutT"
                     /locus_tag="JN755_000405"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7379318.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(31084..33789)
                     /gene="secA"
                     /locus_tag="JN755_000406"
     CDS             complement(31084..33789)
                     /gene="secA"
                     /locus_tag="JN755_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7379319.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(33851..34363)
                     /gene="secM"
                     /locus_tag="JN755_000407"
     CDS             complement(33851..34363)
                     /gene="secM"
                     /locus_tag="JN755_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7379320.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(34594..35511)
                     /gene="lpxC"
                     /locus_tag="JN755_000408"
     CDS             complement(34594..35511)
                     /gene="lpxC"
                     /locus_tag="JN755_000408"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7379321.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(35612..36763)
                     /gene="ftsZ"
                     /locus_tag="JN755_000409"
     CDS             complement(35612..36763)
                     /gene="ftsZ"
                     /locus_tag="JN755_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7379322.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(36824..38086)
                     /gene="ftsA"
                     /locus_tag="JN755_000410"
     CDS             complement(36824..38086)
                     /gene="ftsA"
                     /locus_tag="JN755_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7379323.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(38083..38913)
                     /gene="ftsQ"
                     /locus_tag="JN755_000411"
     CDS             complement(38083..38913)
                     /gene="ftsQ"
                     /locus_tag="JN755_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7379324.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(38915..39835)
                     /locus_tag="JN755_000412"
     CDS             complement(38915..39835)
                     /locus_tag="JN755_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7379325.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(39828..41303)
                     /gene="murC"
                     /locus_tag="JN755_000413"
     CDS             complement(39828..41303)
                     /gene="murC"
                     /locus_tag="JN755_000413"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7379326.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGFDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(41357..42424)
                     /gene="murG"
                     /locus_tag="JN755_000414"
     CDS             complement(41357..42424)
                     /gene="murG"
                     /locus_tag="JN755_000414"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7379327.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(42421..43665)
                     /gene="ftsW"
                     /locus_tag="JN755_000415"
     CDS             complement(42421..43665)
                     /gene="ftsW"
                     /locus_tag="JN755_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7379328.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(43665..44981)
                     /gene="murD"
                     /locus_tag="JN755_000416"
     CDS             complement(43665..44981)
                     /gene="murD"
                     /locus_tag="JN755_000416"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7379329.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(44984..46066)
                     /gene="mraY"
                     /locus_tag="JN755_000417"
     CDS             complement(44984..46066)
                     /gene="mraY"
                     /locus_tag="JN755_000417"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7379330.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(46060..47418)
                     /gene="murF"
                     /locus_tag="JN755_000418"
     CDS             complement(46060..47418)
                     /gene="murF"
                     /locus_tag="JN755_000418"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7379331.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(47415..48902)
                     /gene="murE"
                     /locus_tag="JN755_000419"
     CDS             complement(47415..48902)
                     /gene="murE"
                     /locus_tag="JN755_000419"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7379332.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(48889..50655)
                     /locus_tag="JN755_000420"
     CDS             complement(48889..50655)
                     /locus_tag="JN755_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7379333.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(50671..51036)
                     /gene="ftsL"
                     /locus_tag="JN755_000421"
     CDS             complement(50671..51036)
                     /gene="ftsL"
                     /locus_tag="JN755_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7379334.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(51033..51974)
                     /gene="rsmH"
                     /locus_tag="JN755_000422"
     CDS             complement(51033..51974)
                     /gene="rsmH"
                     /locus_tag="JN755_000422"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7379335.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(51976..52434)
                     /gene="mraZ"
                     /locus_tag="JN755_000423"
     CDS             complement(51976..52434)
                     /gene="mraZ"
                     /locus_tag="JN755_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7379336.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12527 a        14793 c        13098 g        12555 t
ORIGIN      
        1 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
       61 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
      121 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
      181 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
      241 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
      301 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
      361 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
      421 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
      481 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
      541 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
      601 gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat ctttgaggat
      661 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
      721 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
      781 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
      841 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
      901 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
      961 caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat
     1021 aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct ataattaatt gctactaaaa
     1081 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
     1141 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     1201 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     1261 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     1321 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     1381 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     1441 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     1501 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     1561 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     1621 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     1681 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     1741 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     1801 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     1861 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     1921 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     1981 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     2041 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     2101 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     2161 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     2221 caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     2281 tgacgaacac gcctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     2341 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     2401 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     2461 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     2521 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     2581 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     2641 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     2701 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     2761 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     2821 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     2881 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     2941 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     3001 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     3061 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     3121 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     3181 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     3241 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     3301 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     3361 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     3421 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     3481 gccggatgat gtgaaataac cataatgccc ggagaaatcc gggcatattt ccgaatacgg
     3541 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     3601 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     3661 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     3721 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     3781 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     3841 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     3901 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     3961 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     4021 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     4081 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     4141 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
     4201 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     4261 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     4321 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     4381 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     4441 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     4501 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     4561 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     4621 agaccagcca gccccatcgc cacctgtcgc cggtcttgcc atgttgatgt ggatggaacc
     4681 agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
     4741 taattccctg tgggccgccg tcgacgtcac ccggtacttc cagcccgtgc cagtgcaacg
     4801 tcgtctcttc cgccagttgg ttgtagatat caaccgttac cgctttgccg cgctgtaatt
     4861 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
     4921 aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
     4981 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc cacagtggca
     5041 aggctgaagc cacacccagt gcgacggaat atttcaagaa atcacgacgt tgcatagtta
     5101 tttccttatt cttaagcagg caaaacaaaa tcaaacgttg agcatagtcc ttccccttac
     5161 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
     5221 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
     5281 agtgttattc ggcagcctga tcgccgtttg cgcaaacagt tacgcgctca gcgagtctga
     5341 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
     5401 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
     5461 gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag acagctaccg
     5521 cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg cccgcgattc
     5581 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
     5641 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
     5701 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
     5761 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat
     5821 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
     5881 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
     5941 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
     6001 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt
     6061 ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc gctttcaact
     6121 agtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
     6181 ctccgactgc accgatccta tcggtcccgg cgaaagcctg ttcacctcag cattttatga
     6241 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
     6301 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
     6361 tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc
     6421 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
     6481 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
     6541 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
     6601 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
     6661 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
     6721 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
     6781 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
     6841 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
     6901 agaaacggtg gttgctcatc tcgataaaag tcatatttgc gtacatacct acccggaaag
     6961 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
     7021 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
     7081 cattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc actttatcga
     7141 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
     7201 ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
     7261 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
     7321 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
     7381 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
     7441 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
     7501 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
     7561 ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg tgtattcatg ccctgcccgt
     7621 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
     7681 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
     7741 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
     7801 tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat cgcagcctta
     7861 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
     7921 caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     7981 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     8041 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     8101 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     8161 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     8221 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     8281 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     8341 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     8401 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     8461 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     8521 gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     8581 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     8641 caaggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     8701 gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     8761 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     8821 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     8881 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     8941 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     9001 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     9061 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     9121 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     9181 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     9241 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     9301 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     9361 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     9421 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     9481 aagtgcttca cgagctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     9541 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     9601 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     9661 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     9721 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     9781 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     9841 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     9901 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     9961 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    10021 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    10081 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    10141 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    10201 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    10261 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    10321 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    10381 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    10441 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    10501 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    10561 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    10621 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    10681 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    10741 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    10801 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    10861 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    10921 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    10981 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    11041 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    11101 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    11161 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    11221 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    11281 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    11341 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    11401 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    11461 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    11521 aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa taccgtttta
    11581 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    11641 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    11701 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    11761 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    11821 gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    11881 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    11941 gtcaacaatt tcaacagtat caccggtgag catcttactg ataagaatat ggcatggcgg
    12001 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    12061 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    12121 agccagcgtc aggcgacagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    12181 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    12241 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca gcaaaaggac
    12301 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    12361 atgcagacta acccgcagat ccagcagcga agggaggcag cgcgccagcg tattcagtcc
    12421 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    12481 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    12541 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    12601 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    12661 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    12721 acagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    12781 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    12841 attacttctt cttcgctttc gggttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    12901 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    12961 tgtcttcagc atcacaaccc atttcgattg ccagcccgat ttcacccagc agctcgccgc
    13021 cgttggtacc aacaatcgca ccaccgatca cgcggtgaga ttctttgtcg aaaatcagct
    13081 tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc cacgggaagg
    13141 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    13201 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    13261 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    13321 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    13381 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
    13441 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg
    13501 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    13561 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    13621 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    13681 ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    13741 ggcgttctgg cacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    13801 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    13861 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    13921 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    13981 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    14041 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    14101 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    14161 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    14221 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    14281 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    14341 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    14401 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    14461 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    14521 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    14581 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    14641 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    14701 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    14761 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    14821 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    14881 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    14941 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    15001 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga
    15061 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    15121 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    15181 gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc
    15241 tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcgccaa
    15301 acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg ccagtcgccg
    15361 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    15421 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    15481 ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt
    15541 tgccttccgc tttcgcagcc ggagctgctg ccggagcttc agcttttgct gccggagccg
    15601 gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc tgcgccttca acttcgaaga
    15661 tcataatcag cgaaccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga
    15721 cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg gtaatcagtg
    15781 actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact tcaacttcgt
    15841 caccgccgat atccggaacg ttaacttctt tcacgccagc tgctggtgcg ggggtcgctg
    15901 ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga
    15961 agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca
    16021 cggtacctgc aaacggagcc ggaacttcca tagaagcctt gtcgccttct acggtgatca
    16081 gcgactgttc agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt
    16141 cgtcgctgcc gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgcta
    16201 ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg
    16261 cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga
    16321 tctctttaac gatacccgcc tgcggagatg gaacttccat agaggctttg tcgccttcta
    16381 cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga
    16441 tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac
    16501 ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc
    16561 agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac
    16621 gacataagaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc
    16681 gaagccatca gtacccagta cgcgatagtc gtcagccggt acgtaagtac ggacctgctc
    16741 agcgaacagt ttcatatagt cggtagatgc cactgccggc gcgtcgttca tcacctgagc
    16801 gatatacgga acgcgcggag tttccagcgg atgcagcatg ttccagcgtt cacaatcctg
    16861 accatcacgc gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta
    16921 atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatggaac cggagcccag
    16981 cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat
    17041 accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtagtgat
    17101 gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat
    17161 gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggga tagtcagcga
    17221 ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg tggtacgacc
    17281 ggaagtaccg ccgatcagga agccacgcgc ttgctggtct ccagccgccc agcacagatc
    17341 gccgatacgc tggaagccga acatcgagta atagatgtag aatgggatca tcggcagatt
    17401 gttcgtgctg taagaggtcg ccgctgccag ccaggaacac cctgcgccca gctcgttgat
    17461 cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct gctcgcggtc
    17521 ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc
    17581 cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt
    17641 gttcttcagc atcacgttca gagcacgaac gaaagcgata gtggtagaga gctctttgct
    17701 ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa
    17761 gttcggctga cggcttggca gataaccgtg cagtttctga cgctgagcgt gcagataggt
    17821 atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga
    17881 caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac
    17941 ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggtatg
    18001 agcaaggatt actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat
    18061 tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc
    18121 aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc
    18181 tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt
    18241 accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac
    18301 gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt
    18361 gaccgggccg tcaagacgct gcaggttaca gttgataacg aaaaccaggt tatccagttt
    18421 ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc
    18481 gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt
    18541 caggaattta gcctggtaaa tagcaccaat cggacccaga cccatagata cggtcgggaa
    18601 ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac
    18661 ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgggc
    18721 gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt
    18781 gcctgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga
    18841 cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc
    18901 gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg
    18961 ttgttcttca acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt
    19021 tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac
    19081 accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc
    19141 cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt
    19201 tttctggaac ctgtcccatt gaactctcgc cagaaagctc aataagacag gttctacgtt
    19261 tagttaccgc gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccaga
    19321 aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt
    19381 cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc
    19441 gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca
    19501 ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg
    19561 ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcgg
    19621 tgacggcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct
    19681 gcgccagctc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca tcggtactac
    19741 gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat
    19801 actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata
    19861 ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga
    19921 gacgttgaat cgcctcacgc aaggagggac gggagacgtc gaactgtttt gccagttcgc
    19981 gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt
    20041 gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct
    20101 gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag
    20161 taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca
    20221 ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt
    20281 acgtaggaaa gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc
    20341 acttaccaat tttttgattt ttaagtcaat aaaaacgaat ttaaattcac tctgcatatt
    20401 gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca
    20461 tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt
    20521 ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata caacaaacgg aattgcaaac
    20581 ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga
    20641 aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat
    20701 cgcgctgggt ggcgcgatag ggacagggct attcctgggt agcgcctccg taatacagtc
    20761 cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat
    20821 gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc
    20881 ttataaatac tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta
    20941 cgttttagtt gccatggcag agctgactgc cgtgggtaaa tacattcagt tctggtatcc
    21001 ggaaatccca acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct
    21061 gaccaacgtt aaagtgtttg gcgagatgga gttctggttt gccattatca aagttattgc
    21121 ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggcaacg gcggcccgca
    21181 ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg ccgcacggct tcaccggact
    21241 ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac
    21301 cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc aggttatcta
    21361 ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac
    21421 ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt
    21481 tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg
    21541 cgtatattgc aacagccgta tgctgtttgg tctggcacaa caggggaacg cgccaaaagc
    21601 gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt ctgcgctggt
    21661 tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg gcctgttaat
    21721 ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa
    21781 gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc
    21841 gctgggtaac tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac
    21901 cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg
    21961 ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct
    22021 cacccgtaca gggtgagggc aataatctct acaccaacgc cccgtaaatc gtcagtagcg
    22081 caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg
    22141 tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaata
    22201 cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt aacgctttct
    22261 caccatgacc gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta
    22321 catgaagcac tgcatcaatg ccagactgta aacgatgatg cggcgtctgg tatcgcgcca
    22381 gccagtattg ccatgcacgc aagaacgcat accccatcag cgtaacgggt ccccaggttc
    22441 cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc cacagcaatg
    22501 cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca
    22561 tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa gcagctgtca
    22621 gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca
    22681 gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa
    22741 aagtcacacc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca
    22801 cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca
    22861 ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac
    22921 cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc gatccggcgc
    22981 aatatcacaa tggcccgtca tgttattagc gatatcggga tagcgatcta tcagtgcccg
    23041 cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagagtat cggtgccttc
    23101 aagctcaatc ccaatagaaa aatcattgca gcgttcacgc ccctgatact gagagactcc
    23161 cgcatgccag gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat
    23221 caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg
    23281 atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag
    23341 gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccaggcggc aatcgtaatg
    23401 tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct
    23461 ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta
    23521 gctatctgga gttttaacat gccgcctcgc cgctacaacc ctgacacccg acgtgacgag
    23581 ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat
    23641 ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaattct
    23701 cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgtggcaa acgctgggtt
    23761 gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac
    23821 ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg
    23881 ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag
    23941 gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa
    24001 accttacctg gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat
    24061 caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat tgcctccggc
    24121 tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc cggatgcgcc agtagaagtc
    24181 gaagtagaga atctggaaga acttgatgag gccctgaaag ccggagccga tatcatcatg
    24241 ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg
    24301 ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc
    24361 gtggacttta tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg
    24421 cgttttcgct aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc
    24481 cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa
    24541 cctcggaatc tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc
    24601 aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact
    24661 tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc
    24721 ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta
    24781 ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct gcgacggtgg
    24841 cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa
    24901 gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc
    24961 aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga
    25021 cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc
    25081 ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag tcttgctgga
    25141 tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg agttgctgga
    25201 cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat
    25261 ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg agaagcatca
    25321 gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga
    25381 tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt
    25441 gcatccttta ccggatgttt caccggatgc cggagtcgca ttaacagcca gattaaaagt
    25501 gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga
    25561 actggcagga aacaccgtct catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa
    25621 ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca cgctgggaat
    25681 gcagccgtta caactggcgg actttgctca tgccttgcaa caaccacagg gactggtgct
    25741 ggtaactggc cctacaggca gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct
    25801 gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc ccatagccgg
    25861 actaaaccag acgcaaatcc atccgcgtgc cgggctcacc tttcagggtg ttttgcgtgc
    25921 gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg gcgaaacggc
    25981 agaaattgcc attaaagccg tgcaaaccgg tcacctggtg ttgtccacgc tacacaccaa
    26041 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gttgcccgct ggatgctatc
    26101 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    26161 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    26221 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    26281 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    26341 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    26401 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    26461 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    26521 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    26581 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    26641 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    26701 tggctctgct ggcggaacag catcccagta aacaatggca agcgttgctg caatcgctgg
    26761 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    26821 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    26881 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    26941 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    27001 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    27061 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    27121 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    27181 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    27241 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    27301 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    27361 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    27421 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    27481 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    27541 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    27601 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    27661 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    27721 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    27781 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    27841 cgggcctcgc agcggcagct tgacggtttt accttctgct gcgcggtatt ccgcaacgcc
    27901 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    27961 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    28021 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    28081 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    28141 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    28201 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    28261 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    28321 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    28381 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    28441 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    28501 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    28561 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    28621 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    28681 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    28741 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    28801 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    28861 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    28921 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    28981 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    29041 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    29101 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    29161 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    29221 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    29281 cctatgtact gtgggttgtg ccattgctga tagaaaactc actgtataaa aaagcgaatc
    29341 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    29401 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    29461 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    29521 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    29581 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    29641 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    29701 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    29761 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    29821 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggg
    29881 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    29941 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    30001 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    30061 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    30121 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    30181 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    30241 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    30301 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    30361 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    30421 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    30481 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    30541 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    30601 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    30661 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    30721 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    30781 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    30841 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    30901 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    30961 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    31021 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    31081 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    31141 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    31201 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    31261 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    31321 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    31381 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    31441 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    31501 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    31561 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    31621 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    31681 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    31741 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    31801 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    31861 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    31921 cacccacggg tgttcaatgg cttcgcctag cttcataccc agtttacgca tcatgccgga
    31981 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    32041 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    32101 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    32161 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    32221 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    32281 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    32341 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    32401 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    32461 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    32521 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    32581 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    32641 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    32701 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    32761 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    32821 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    32881 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    32941 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    33001 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    33061 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    33121 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    33181 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    33241 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    33301 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    33361 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    33421 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    33481 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    33541 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    33601 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    33661 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    33721 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    33781 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    33841 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    33901 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    33961 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    34021 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    34081 aagaaagatg acggattacc gtgcgaatag catgttgatg ccagtaatca acggaatagt
    34141 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    34201 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    34261 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    34321 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    34381 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    34441 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    34501 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    34561 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    34621 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    34681 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    34741 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    34801 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    34861 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    34921 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    34981 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    35041 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    35101 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    35161 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    35221 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    35281 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    35341 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    35401 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    35461 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    35521 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    35581 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    35641 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    35701 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    35761 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    35821 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    35881 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    35941 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    36001 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    36061 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    36121 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    36181 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    36241 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    36301 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    36361 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    36421 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    36481 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    36541 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    36601 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    36661 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    36721 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    36781 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    36841 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    36901 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    36961 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    37021 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    37081 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    37141 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    37201 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    37261 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    37321 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    37381 cagcataagg aattacctta gtgtggcgta atgccccacc ggtataaacg gcgatatcca
    37441 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    37501 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    37561 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    37621 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    37681 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    37741 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    37801 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    37861 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    37921 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    37981 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    38041 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    38101 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    38161 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    38221 ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat cgccccggcc
    38281 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    38341 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    38401 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    38461 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    38521 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    38581 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    38641 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    38701 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    38761 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    38821 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    38881 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    38941 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    39001 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    39061 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    39121 cctgcaaatt ggcctcttgt gacgctttca gacctgcggg gcagaaatac tgtgtctcat
    39181 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    39241 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    39301 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    39361 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    39421 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    39481 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    39541 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    39601 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    39661 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    39721 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    39781 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    39841 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    39901 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    39961 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    40021 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    40081 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    40141 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    40201 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    40261 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    40321 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    40381 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    40441 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    40501 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    40561 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    40621 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    40681 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    40741 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    40801 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    40861 gggtcgaacc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    40921 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    40981 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    41041 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    41101 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    41161 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    41221 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    41281 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    41341 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    41401 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    41461 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    41521 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    41581 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    41641 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    41701 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    41761 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    41821 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    41881 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    41941 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    42001 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    42061 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    42121 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccagccgt
    42181 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    42241 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    42301 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    42361 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    42421 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    42481 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    42541 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    42601 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    42661 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    42721 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    42781 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    42841 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    42901 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    42961 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    43021 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    43081 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    43141 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    43201 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    43261 tgccccttta acagcgctac ctactaccag cacgatcatc agcaggatga tagatccgag
    43321 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    43381 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    43441 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    43501 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    43561 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca agatactgaa
    43621 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    43681 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    43741 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    43801 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    43861 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg cgccagtggg
    43921 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    43981 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    44041 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    44101 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccgcc
    44161 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    44221 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    44281 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    44341 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    44401 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    44461 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    44521 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    44581 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    44641 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    44701 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    44761 ccgggactgg cgacaatcag atctgccgcc atcaaccatt catcattcag actgcccgtg
    44821 tggcgttcta cggcttcggg taatttatcc aggctaggcg gtgtcatacg cgtatccata
    44881 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    44941 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    45001 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    45061 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    45121 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    45181 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    45241 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    45301 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    45361 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    45421 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    45481 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    45541 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    45601 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    45661 agaaatattt ccaacgagcg atcaaccctt tggtatcttt acgcaccact ttgcgataat
    45721 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    45781 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    45841 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    45901 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    45961 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    46021 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    46081 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    46141 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    46201 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    46261 gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagt cttcgctttc
    46321 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    46381 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    46441 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    46501 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    46561 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    46621 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    46681 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    46741 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    46801 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    46861 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tcctggcaat
    46921 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    46981 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    47041 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    47101 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    47161 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    47221 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    47281 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    47341 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    47401 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    47461 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    47521 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    47581 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    47641 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    47701 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    47761 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    47821 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    47881 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    47941 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    48001 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    48061 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    48121 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    48181 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    48241 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    48301 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    48361 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    48421 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    48481 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    48541 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    48601 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    48661 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    48721 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    48781 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    48841 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    48901 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    48961 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    49021 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    49081 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    49141 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    49201 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    49261 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    49321 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    49381 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    49441 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    49501 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta acgccagctt
    49561 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    49621 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    49681 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    49741 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    49801 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    49861 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcattgt tcagttcgcg
    49921 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    49981 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    50041 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    50101 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    50161 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    50221 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    50281 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    50341 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    50401 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    50461 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    50521 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    50581 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    50641 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    50701 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    50761 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    50821 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    50881 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    50941 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    51001 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    51061 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    51121 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    51181 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    51241 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    51301 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    51361 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    51421 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    51481 gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc gatatcggct
    51541 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    51601 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    51661 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    51721 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    51781 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    51841 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    51901 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    51961 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    52021 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    52081 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    52141 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    52201 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac gcgataattt
    52261 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    52321 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    52381 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    52441 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    52501 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    52561 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    52621 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    52681 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    52741 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    52801 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    52861 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    52921 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DADLTI010000007        50387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLTI010000007 DADLTI010000000
VERSION     DADLTI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.007"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..373
                     /locus_tag="JN755_000424"
     CDS             <1..373
                     /locus_tag="JN755_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379337.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JN755_000425"
     CDS             484..1407
                     /locus_tag="JN755_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7379338.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JN755_000426"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JN755_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7379339.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JN755_000427"
     CDS             2451..2744
                     /locus_tag="JN755_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379340.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JN755_000428"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JN755_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7379341.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JN755_000429"
     CDS             complement(4186..4638)
                     /locus_tag="JN755_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7379342.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JN755_000430"
     CDS             complement(4691..6025)
                     /locus_tag="JN755_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7379343.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JN755_000431"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JN755_000431"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY7379344.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JN755_000432"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JN755_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7379345.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JN755_000433"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JN755_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7379346.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JN755_000434"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JN755_000434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7379347.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JN755_000435"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JN755_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7379348.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JN755_000436"
     CDS             complement(13037..13327)
                     /locus_tag="JN755_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7379349.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JN755_000437"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JN755_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7379350.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JN755_000438"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JN755_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7379351.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JN755_000439"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JN755_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7379352.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JN755_000440"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JN755_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7379353.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLVTLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JN755_000441"
     CDS             complement(17349..17846)
                     /locus_tag="JN755_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7379354.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JN755_000442"
     CDS             complement(17843..18205)
                     /locus_tag="JN755_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7379355.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JN755_000443"
     CDS             complement(18195..18542)
                     /locus_tag="JN755_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7379356.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JN755_000444"
     CDS             18651..19064
                     /locus_tag="JN755_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379357.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20577)
                     /locus_tag="JN755_000445"
     CDS             complement(19111..20577)
                     /locus_tag="JN755_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7379358.1"
                     /translation="MTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCT
                     PARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGT
                     GECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNR
                     AVDFLQQPVRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPE
                     HHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDH
                     GEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEK
                     PEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDEL
                     YDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPR
                     WMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20588..22303)
                     /locus_tag="JN755_000446"
                     /pseudo
     CDS             complement(20588..22303)
                     /locus_tag="JN755_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22470..23363
                     /locus_tag="JN755_000447"
     CDS             22470..23363
                     /locus_tag="JN755_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7379359.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23360..24174)
                     /locus_tag="JN755_000448"
                     /pseudo
     CDS             complement(23360..24174)
                     /locus_tag="JN755_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24174..25790)
                     /locus_tag="JN755_000449"
                     /pseudo
     CDS             complement(24174..25790)
                     /locus_tag="JN755_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26085..26759
                     /locus_tag="JN755_000450"
     CDS             26085..26759
                     /locus_tag="JN755_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7379360.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26798..28459)
                     /locus_tag="JN755_000451"
     CDS             complement(26798..28459)
                     /locus_tag="JN755_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7379361.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JN755_000452"
     CDS             complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JN755_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7379362.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JN755_000453"
     CDS             complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JN755_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7379363.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            29913..30245
                     /locus_tag="JN755_000454"
     CDS             29913..30245
                     /locus_tag="JN755_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7379364.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30247..31497)
                     /locus_tag="JN755_000455"
     CDS             complement(30247..31497)
                     /locus_tag="JN755_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7379365.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            31562..32580
                     /locus_tag="JN755_000456"
                     /pseudo
     CDS             31562..32580
                     /locus_tag="JN755_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32541..33878)
                     /locus_tag="JN755_000457"
     CDS             complement(32541..33878)
                     /locus_tag="JN755_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379366.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33953..35101)
                     /gene="dgoD"
                     /locus_tag="JN755_000458"
                     /pseudo
     CDS             complement(33953..35101)
                     /gene="dgoD"
                     /locus_tag="JN755_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35098..35715)
                     /locus_tag="JN755_000459"
     CDS             complement(35098..35715)
                     /locus_tag="JN755_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7379367.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35699..36577)
                     /locus_tag="JN755_000460"
     CDS             complement(35699..36577)
                     /locus_tag="JN755_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7379368.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36574..37263)
                     /gene="dgoR"
                     /locus_tag="JN755_000461"
     CDS             complement(36574..37263)
                     /gene="dgoR"
                     /locus_tag="JN755_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7379369.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37541..38197
                     /locus_tag="JN755_000462"
     CDS             37541..38197
                     /locus_tag="JN755_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379370.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38243..39055)
                     /gene="yidA"
                     /locus_tag="JN755_000463"
     CDS             complement(38243..39055)
                     /gene="yidA"
                     /locus_tag="JN755_000463"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY7379371.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39169..39576)
                     /locus_tag="JN755_000464"
     CDS             complement(39169..39576)
                     /locus_tag="JN755_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7379372.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39807..42221)
                     /gene="gyrB"
                     /locus_tag="JN755_000465"
     CDS             complement(39807..42221)
                     /gene="gyrB"
                     /locus_tag="JN755_000465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7379373.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42250..43323)
                     /gene="recF"
                     /locus_tag="JN755_000466"
     CDS             complement(42250..43323)
                     /gene="recF"
                     /locus_tag="JN755_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7379374.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43323..44423)
                     /gene="dnaN"
                     /locus_tag="JN755_000467"
     CDS             complement(43323..44423)
                     /gene="dnaN"
                     /locus_tag="JN755_000467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7379375.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44428..45831)
                     /gene="dnaA"
                     /locus_tag="JN755_000468"
     CDS             complement(44428..45831)
                     /gene="dnaA"
                     /locus_tag="JN755_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7379376.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46128..46208
                     /locus_tag="JN755_000469"
     CDS             46128..46208
                     /locus_tag="JN755_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379377.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46438..46578
                     /gene="rpmH"
                     /locus_tag="JN755_000470"
     CDS             46438..46578
                     /gene="rpmH"
                     /locus_tag="JN755_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7379378.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46595..46954
                     /gene="rnpA"
                     /locus_tag="JN755_000471"
     CDS             46595..46954
                     /gene="rnpA"
                     /locus_tag="JN755_000471"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7379379.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46918..47175
                     /gene="yidD"
                     /locus_tag="JN755_000472"
     CDS             46918..47175
                     /gene="yidD"
                     /locus_tag="JN755_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7379380.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47178..48824
                     /gene="yidC"
                     /locus_tag="JN755_000473"
     CDS             47178..48824
                     /gene="yidC"
                     /locus_tag="JN755_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7379381.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48930..50294
                     /gene="mnmE"
                     /locus_tag="JN755_000474"
     CDS             48930..50294
                     /gene="mnmE"
                     /locus_tag="JN755_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7379382.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12434 a        13548 c        12707 g        11698 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaacggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgccactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcttg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggtaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgtcagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg
    19681 aatacgggtg atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat
    19741 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19801 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19861 atggtgataa agcccgtcat caccgactgg cgatggcatc gcctgcgccc ataagcggtg
    19921 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19981 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    20041 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20101 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20161 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20221 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20281 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20341 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20401 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20461 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20521 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20581 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20641 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20701 tcgctaacca gcgcgtaccg tagccgccga attcagccag cccgacatag acgccaatca
    20761 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20821 caaacgcatc tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca
    20881 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20941 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    21001 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21061 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21121 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21181 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21241 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21301 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21361 tgtcggcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21421 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21481 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21541 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21601 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21661 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21721 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21781 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21841 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21901 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21961 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    22021 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22081 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22141 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22201 agccttgttg cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca
    22261 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    22321 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca
    22381 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    22441 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa
    22501 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    22561 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
    22621 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    22681 gtgggaaaat tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat
    22741 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact
    22801 ggatggaatt tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt
    22861 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
    22921 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    22981 gtcttatgtc tgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    23041 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    23101 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    23161 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
    23221 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    23281 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
    23341 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
    23401 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
    23461 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
    23521 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
    23581 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
    23641 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
    23701 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt
    23761 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
    23821 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gccgtgggat
    23881 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca taacaaaatc
    23941 aacgtcgctg aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
    24001 ttctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc
    24061 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
    24121 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
    24181 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
    24241 tcacgcaatt gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
    24301 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
    24361 agtgcaatac gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
    24421 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt
    24481 tagcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
    24541 cccggcgtgc acatgttgaa ctgcagaatc agggggcgga aaaccacgaa gtacagcagg
    24601 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
    24661 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
    24721 ccaaagagat acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc
    24781 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg
    24841 gttaaggttg cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg
    24901 aagtacatcg ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca
    24961 aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga
    25021 tgctgcgccc agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt
    25081 ccgtagatga agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc
    25141 cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt
    25201 acttttggcc agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata
    25261 atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac
    25321 agacggttat gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta
    25381 tcgagggttt taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg
    25441 aaatcgacgc cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag
    25501 gtcaggaaac tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca
    25561 atgggtaaac cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct
    25621 tcaataatgt gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca
    25681 aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc
    25741 agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc
    25801 tcttttctca attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg
    25861 ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc
    25921 ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg
    25981 tacacagcat cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt
    26041 gtgggcgcat tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca
    26101 ttgcggagcg gttaagaatt cgacttaatt ccgcggattt cacgctaaac ggccttcttc
    26161 ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca
    26221 ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg
    26281 tgcgcaaaga tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac
    26341 ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    26401 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    26461 gttttgtgga agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc
    26521 gtaacctttc actgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    26581 ggattttgat tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg
    26641 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    26701 gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg
    26761 tggagtacca tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca
    26821 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    26881 aagagagcgc cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag
    26941 gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    27001 tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa
    27061 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac
    27121 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    27181 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    27241 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa
    27301 tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt
    27361 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    27421 ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga
    27481 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    27541 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    27601 tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta
    27661 aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    27721 aaggtacttt tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga
    27781 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    27841 tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct
    27901 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    27961 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    28021 ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg
    28081 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    28141 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    28201 gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca
    28261 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    28321 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    28381 gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    28441 ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt
    28501 atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg
    28561 ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc
    28621 cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc
    28681 tgatagcgat acgctgcgct gcgatgggtt ctggctcatt acgaattaaa tcaatatgca
    28741 gtaaaccgtt tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca
    28801 ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct
    28861 ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac
    28921 ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt
    28981 acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac
    29041 cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta
    29101 agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg
    29161 gagatgtcag gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt
    29221 ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag
    29281 ccccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga
    29341 tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca
    29401 gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag
    29461 caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac
    29521 tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt
    29581 aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta
    29641 agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga
    29701 ctacgatgta aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt
    29761 gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc
    29821 cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag
    29881 gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca
    29941 tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg
    30001 ggaaagaagg aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa
    30061 ccaactgggg ctctaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca
    30121 cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa
    30181 aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata
    30241 actgatttag cgatcggttt cggtgatcca cagctggcca ccttccatcc acgccagcca
    30301 ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt
    30361 taaatactca acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa
    30421 gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg
    30481 ggttaacttg cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt
    30541 atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg
    30601 cgggacgttg actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac
    30661 gacgccacgc ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc
    30721 cgatttccag ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc
    30781 gccctttggc gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc
    30841 ttcataagca cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac
    30901 cagcacacac cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg
    30961 gccaaacggt gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat
    31021 tacatggtcg ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg
    31081 gctgccaccg cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt
    31141 gctcaccttg ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt
    31201 ttcatcagga ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt
    31261 caccacgccg acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg
    31321 gatattcaca cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa
    31381 taccagccac tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg
    31441 agcaaaggtt atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact
    31501 tcacaaggac ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa
    31561 gatggaacat ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg
    31621 aaagttagcc ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga
    31681 aggtttcagc aaaccttgca gattttcagc gtcaaaactg tcgacgtcgc cgcatcgctc
    31741 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg
    31801 aaatcgctga tccccgccag cgttgaggtt tatcacgata gcctgtgccg aaaaatctgg
    31861 cgtgaggatg ataaatggca tgtcattttt cgtgcagacg gctgggagca acatattacc
    31921 gcccgctatc tggtcggtgc cgatggcgca aactcgatgg tgcggcgaca tctctacccg
    31981 gatcatcaaa tccgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccggtg
    32041 ccgttctact cctgcatctt tgataatgcg ataactgact gttactcatg gagtatcagc
    32101 aaagatggtt attttatctt tggcggtgcc tatccaatga aagacggtca gacgcgtttc
    32161 acgacgctga aagagaaaat gagcgccttt cagttccagt ttggcaaagc ggtgaaaagt
    32221 gagaaatgca cggtgttgtt tccctcacgc tggcaggatt ttgtctgtgg taaggacaac
    32281 gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    32341 tatgcgctgg atagcgcaga gattctgcgt gcggtgttcc tgaaacagcc ggagaagagc
    32401 aacgccgctt actggcgcgc cacacgcaaa ctgcgtttaa aactcttcgg caagatagta
    32461 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg
    32521 catattccac agttgaaaga ttatccaacg cgcttcacat cacccaccag caggatgtaa
    32581 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggcgc gaaaccgtaa
    32641 ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag
    32701 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    32761 caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc
    32821 atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata
    32881 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    32941 cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct
    33001 gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca ggttaagaaa
    33061 aaccacagtg cagaagccac cgcaaattgc ccaagataaa cgccgatcag tttacgatgg
    33121 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttctt caccggcgca
    33181 tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatacct
    33241 ttggtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    33301 ccaccagtga caatgaacac ccagtgccag ctcaacatct cctgaatcca gatcagcagc
    33361 ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg
    33421 cgttcatgtt ccggaaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    33481 tcgaaaatac cggttatcgc acgcagacca attaatgaca ttaatcccgt ggcaaagccc
    33541 tggaataaag tcgccactga ccaaccaaat atcgcaataa aataagtcac gcgagaacct
    33601 acgcgatcta aaaaccaacc gccggggatc tggcatagcg tataaagcca ggcgaaggcc
    33661 gaaaatacat agcccatttc cgctttcgta atgccgaact cttcctgaat atgggcggaa
    33721 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    33781 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    33841 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    33901 atatttttgt attgctgttt tagtgcccga tgaggggctt acgtggcagg aattaccact
    33961 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    34021 tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaacggtt
    34081 taaagaagcc gccaaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    34141 cgcctttgtt gtaatgaatg cccatacttt gttactgaag taccgcgtta taggaaacaa
    34201 agtcgatatg caggcaagcc gccagtgcaa tcggtccgaa cggacagtgc ggcgcaaggg
    34261 tcacatcata ggcttctgcc attccagcaa ttttgtagca ttcggtaata ccgcctgcat
    34321 gggagagatc cggttgcaga atcgaaatac cgcctgcctc cagcacgcgt ttaaaatcga
    34381 aacgtgagaa catgcgctca cccgccgcca gtggaatatg cgtttgtgcc gccaatttcg
    34441 ggtagtattc ggcttgttcc gccagcaccg gctcttcaat aaacagcggg cgatacggct
    34501 ccagttcttt aatcagcact ttcgccattg gcgcgctgac gcgaccgtgg aaatcaagac
    34561 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    34621 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    34681 cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    34741 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagactg
    34801 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc
    34861 tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcgt
    34921 tgatgcgcga aggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca
    34981 ccgtacgggc gcggccttca atcacgggct cgccccaacc gaccacgcct tcatcggttt
    35041 caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca
    35101 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac
    35161 ggactgcccg gcgcgataaa gatcgctgcc taagcccgcc ccaacacaac ctgcgtctat
    35221 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    35281 taataccact tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    35341 cagcgcctgc gcgcccgctt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg
    35401 gcaaacggtc atgccgtagc ccaccgcgcg gcggatcact tcactatgga tattgggcgt
    35461 aacgatgagc tgacagccca tcctggcgag cgcatcgacc tgttcaggtt tcagtaccgt
    35521 acctgcgcca atcaacgcct tgtcgccata cgcatcaacg atggcgggaa tgctctgctc
    35581 ccattgtggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    35641 aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    35701 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca
    35761 ccgccgccac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    35821 ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct
    35881 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcggaga agtgttccca
    35941 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggcgca ttaaggccgc
    36001 gctcaaggcc agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    36061 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    36121 tgatttgctg gctatcggcc tgcacccatt tgcaatgcgt accgggcata acataaagag
    36181 aggaaggagc cagagctcgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    36241 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccaacagacg
    36301 ttaattgttc gccaatagac gaaaaacggg caggaacaga taaatacgga gcaactttcc
    36361 agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattttct tcacgccagt
    36421 cggtcgtgac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    36481 cttctgattg cctgctctcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    36541 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    36601 cgagctggca atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat
    36661 cgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    36721 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa
    36781 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    36841 cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag
    36901 atcgctggag gtcgcgcgct ctgctgccca acgggcaatc gccggttcca ccagatttcg
    36961 cacttcgctc atggcactga taagccgtgg gtcgtagtcg ttttccagca cccattgcag
    37021 tacgtcagtg tcgaggtaat tccactggtt acgcggtgcc acaaacgccc cgcgataacg
    37081 tttcatttca atcagccgct tcgccatcag cgaacggaac acctcacgga tgatgttgcg
    37141 cgaggttgca aactcttcac agagctccgc ctcagccgga agtggcgagc caggtacgta
    37201 tttgccgtga acgatctgtt tacccagcgt aatgacaatg cgatcggttt tattgagagt
    37261 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    37321 cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ccgtgcgtgt aatgtagtac
    37381 aaacttatat tgttgtgcta caatttagat caaaaaaaga acaatgtata aaaaaatgac
    37441 atgcgtcggg cagaaatctg aaaagggata tcaggcgcta aacaggaggg aaagaagagt
    37501 atgctttcaa cggcttagct actcgtttaa aagattaatc atgaagttga attttaaggg
    37561 attttttaag gctgccggtt tattcccact ggcgctgatg ctttcaggct gtatctcgta
    37621 tgctctggtt tcccataccg caaagggtag ttcaggaaag tatcaatcgc agtcagacac
    37681 catcactggg ctatcgcagg caaaagatag taatggaaca aaaggctatg tttttgtagg
    37741 ggaatcgctg gattacctta tcactgatgg tgccgatgac atcgttaaga tgctcaatga
    37801 tccagcactt aaccggcaca atattcaggt tgccgatgac gcaagatttg ttttaaatgc
    37861 ggggaaaaag aaatttaccg gcacaatatc gctttactac tactggaata acgaagaaga
    37921 aaaggcactg gcaacgcatt atggttttgc ctgtggtgtt caacactgta ccaggtcact
    37981 ggaaaaccta aaaggcacaa tccatgagaa aaataaaaac atggattact caaaggtgat
    38041 ggcgttctac catccattta aagtgcgatt ttatgaatac tattcaccca gaggcattcc
    38101 ggatggtgtt tccgcagcat tactgccagt gactgttacg ctggacatca ttactgcacc
    38161 gctgcaattt ctggttgtat atgcagtaaa ccaataatca gtaagcgggc aaacgcgttt
    38221 atgctgtttg cccgcccaca gattaattca gcacatactt ctcaatagca aacgccacgc
    38281 catcttcaag gttggatttt gtgacaaagt tcgccatttc tttcaccgaa ggaatagcgt
    38341 tatccatcgc cacaccgacg cctgcatatt caatcattgc gatgtcgttt tcctgatcgc
    38401 caatcgccat gatttcttcc ggtttaatac ctaacacgtc ggccagtgat ttcacacccg
    38461 tacctttgtt aacgcgttta tcgaggattt cgaggaagta cggcgcactt ttcagcacgg
    38521 tatatttctc tttcacttcc tgcggaatac gcgcgatagc ctggtcgagg atggcgggtt
    38581 catcaatcat catcactttc aggaactggg tattggggtc cattttctcc gcttcgcaga
    38641 acaccagcgg aattgtggca acgaaggatt catgcaccgt gtagtagctg atatcgcggt
    38701 tagcggtgta cagcgtggtg cggtccaggg cgtggaaatg agaaccgact tcgcgagaga
    38761 gtttttccag gaaacgatag tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat
    38821 cagcggcctt ctgtaccagc gcgccgttat aagtaatgca gtagtcgccc ggctgttcca
    38881 tatgcagctc tttcaggtag ttgtgcacac ctgcatacgg acgacccgtc gttagcacga
    38941 cattcacgcc acgggcgcga gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt
    39001 gatcgggcag cagaagggtg ccatccatat cgatagcaat gagtttgata gccatgagtt
    39061 cccaaggtag attagttcct gacccatgct aacgcgattc cgctcaaaaa tcagtacaac
    39121 acccgaggga aaaggggatg caacgcgggt tgcatgctcc ccatttgctt agcggaagag
    39181 tttccctttc agcagttcca tgcctgcgga aagcagatcg ttattggctt gtgctgacac
    39241 ttcaccttgc ggtgagagcg catcaataat cttcggcaat tgctctgcca gtaaactgga
    39301 agccgtactg gtatccacgc caagtttttg cccgagatcg gacaccgcat ttgtgccgag
    39361 cgccgattcc agttgctcgc cgctaactga ttgatttctc tgttgattac tcaaccaagt
    39421 tgagagaatg gcccctaagc cgccactttg cagtttttcc agcagcacct gaatgccacc
    39481 ctgctcctca acccaactta aaatcgcctg atatttcccc gcatcgcctt tcagaaaggc
    39541 accgacaact tcatcaaaaa gccccatgat aatcacctgt aaagcgttac gtgttgaccc
    39601 aaaaagtata gatctgcggg atgataattg cggattgcag aaataaaaag ggcggagatg
    39661 atctccgccc ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt atccggccta
    39721 caaaatcatg caaaatcaat atattgcagg agtctcgtag gactaataag cgtagcgcat
    39781 caggcacgct cgcatggtta acgccattaa atatcaatat tcgccgcttt cagggcgttc
    39841 tcttcaataa acgcacgacg cggttctacg gcgtcgccca tcagcgtggt gaacaactgg
    39901 tcagcggcaa tcgcatcctt aacggtaacg cgcagcatac gacggctttc cgggtccatg
    39961 gtggtttccc acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag
    40021 aggccacgac gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc
    40081 tgacgacgct cgccacgttc gataaacgca tcttcttcca gcaagccacg cagtttctca
    40141 cccagcgtgc agatacgacg atattcgcca ccggtgataa actcgtgatc cagcggatag
    40201 tcagtatcca caccgtgggt acgcacgcga acaatcggct cgaacaggtt ttgttcggca
    40261 ttggtgtgga catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg
    40321 accagcgcgt tcacccagcg ggtaacggtc tgctcatcag aaaggtcagc ttccgtcaac
    40381 gtcggctgat agataagctc tttcagcatt gctttcggat aacgacgctc catgcggttg
    40441 atcattttct gcgtcgcgtt gtactcagat accagtttct ctaacgcttc gccagccaat
    40501 gccggtgcac tggcgttggt gtgcagcgtt gcgccgtcca gcgcgataga gatctggtac
    40561 tgatccatcg cttcgtcgtc tttaatgtac tgttcctgct tgcctttctt cactttgtac
    40621 agcggcggct gagcgatgta gacgtgaccg cgttcaacga tttccggcat ctgacgatag
    40681 aagaaggtca acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg
    40741 atgatgctgt gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacagcca
    40801 agcgcggtga taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc
    40861 tcgacgttaa ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc
    40921 tgcttcgcag agccgcccgc ggagtcccct tccaccaggt acagttcgga aagcgccgga
    40981 tcgcgttcct ggcagtctgc cagtttgccc ggcaggccag ctaagtcgag cgcaccttta
    41041 cggcgggtca tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc atcgataatt
    41101 ttgccaacca cgattttcgc atcagttggg ttttccagca ggtattccgc cagcagttcg
    41161 ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc tttggtctgg
    41221 gaggagaatt tcgggtccgg cacttttacg gaaacgaccg caatcaggcc ttcacgcgca
    41281 tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc cttctttgtc catgtaggca
    41341 ttcagggtac gggtcatcgc cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc
    41401 ggaatgttgt tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcagcgcc
    41461 acttcaacgc caataccgtc tttttcggtg gagaagtaga agatatttgg gtggatcggc
    41521 gttttgttct tgttcagata ttcaacgaac gctttgatgc cgccttcata gtggaagtgg
    41581 tcttctttgc cgtcacgctt gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac
    41641 aactcacgca gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaggtt
    41701 tcgaggctgg gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc
    41761 agcggagcct gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctga
    41821 ataaccagct ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga
    41881 ccgccggaca ctttatagga gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg
    41941 atcacttccg ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg
    42001 tcatcctgta cagagacaga gttatcggcg tgaatggtga cgataatttc tttacagtga
    42061 cccgcgagcg cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg
    42121 gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct
    42181 ttcaggactt tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt
    42241 atacttgggt taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc
    42301 cgacatgtct ataacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc
    42361 ttttaagcgg ctggcaagca acccgcgacg ctcatcatca agctcagagg caaaatcatc
    42421 tatcaggtag agacaccgcc gcccgctttc acgagtgagg aactctcctt gcgccagacg
    42481 taaggcgcac atcaacagct taagctgccc acgcgataag gtatcttcca ccggcgcacc
    42541 gtcggcgcga atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg ttagctggcg
    42601 atcgcgttca aaattacgtt ccagcacctc agcgtactct gtctctttct cccagccgcg
    42661 ctggaaagag aaagtcagag aaaactcagg gagaaattgc ttacaggtat catccatatc
    42721 ggccgcgata ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat
    42781 cagttcttta tcccacgggc gtagctgttc gtaacgagtc acctggcgca gcgccgcatt
    42841 gcgctgcttg agcaatcgct tgagattgct ccaggcggtg aaaaatctag gttcgttgtg
    42901 aaagcatccc cagtcgagga atgctcttct gtatttgggg ccgccgttga gtaaagtaaa
    42961 cccttctggc gttatcaact gcatcggcat caggtgcgcc agttccgcga ctttatgccc
    43021 gtccgtaccg tcaatacgga ctttgctgtc gccctgtttg tctttggtta agccaatcgc
    43081 cgtctcgcgc tcttcgccct gtaatcgccc gtggagaaca aatgcctcct gctcatggcg
    43141 aatgacgcga ccaatctgca aactgcgaaa cgcccgacca tggccgagcg tatagatggc
    43201 ttccagcacg ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga
    43261 taaagcgaga tccgcggttt caatattgcg gaaatcgcgg atcaacaagc gggtgaggga
    43321 cattacagtc tcattggcat gacaacatag gcagccgact gtgatgcggc atcttcaatc
    43381 tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagcgcgttc
    43441 agaacatcca gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta
    43501 acgtcgagga tctcttccgc ttcttcctgt tccgggttgt tggcagtaat tttcagctgg
    43561 ttttcgctga catacaggcg cacaccgcgg aatttctcgt tagagagaat cgccgcgcga
    43621 gcaaacgcct gcttgagcag atcgcagcct gcttccagat gtttgtccgg gttcttcggc
    43681 agaacgcgac ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa aataaagtcg
    43741 ccaacgtggg cacgaatgtt attgctgcca atctgcacac gcagcagatt gtcgccgccg
    43801 tcgagcatac gcatcagttc aatcacgcct ttacgcggta cgatcaccga atggcttggc
    43861 aaagattgac caattggcat tgaacagacc gccagacggt ggccgtcggt tgccacggtg
    43921 cgcagttctt caccttcggt ttcaaacagc ataccgttta agtaatagcg aacatcctga
    43981 tgcgccatcg aaaactgggt tgcttcaatc agacgcttca tcgtcgcctg cggcagggta
    44041 aattcgactt cgctctgcca gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac
    44101 agcgaaaaac ggctacgccc ggagcgcacc agcatccgtt caccttccag ctgcacggca
    44161 atttccgcgc cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc
    44221 gtcgctcctg gctcgtgtgg ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga
    44281 tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc
    44341 ggtagcgtag gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc
    44401 tcacgttcta cggtaaattt cataggttta cgatgacaat gttctgatta aatttgaaaa
    44461 atcttctttg atatcgtggc tctcttcacg caactgctcg atcttacggc aggcatgaag
    44521 caccgtcgtg tggtcacgcc caccaaacgc atcgccaatc tctggcagac tgtggttagt
    44581 cagctctttc gctagcgcca tcgccatctg gcgcggacgc gccaccgagc gggatcgacg
    44641 cttggaaagg agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt
    44701 gtcgatggtg accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa
    44761 gtcgatggtg atcgcccgtc cggtaaagtt ggcgttagca ataacgcggt tcagcgcccc
    44821 ttccagctca cgtacgttag atcgtagacg cttggcgata aagaacgcca cttcacccgg
    44881 caaacgaatg tcattttcgt cggccttttt catcaggatc gccacacggg tttccagctc
    44941 tggcggttcg atcgccacag tcagtcccca accgaagcgg gatttcaaac gatcctcaac
    45001 gccgttgatc tctttcggat agcgatccga ggtgagaatg atctgttgat taccttccag
    45061 cagggcgttg aaggtgtgga aaaactcttc ctgagatcgt tctttattag caaaaaactg
    45121 aatatcgtcg atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc
    45181 gttgttttgc agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac
    45241 tttggcattc ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt
    45301 acccagaccc gtgccgccat aaaggaacaa cgggttatag gcaccgccag ggttatccgc
    45361 cacctggcga gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa
    45421 cgtgtgtttg acgtttacgt tagaacgata ggtcggttct gccggggccg ggacgttatc
    45481 ccaacctgag cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg
    45541 ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac
    45601 ttcaaaacgc agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt
    45661 attaaggtac ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggacag
    45721 cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg
    45781 taactcatcc tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc
    45841 cactcgaaca aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc
    45901 gaaaagaccc tgcttgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa
    45961 gatcctgcaa aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt
    46021 ttctcacaga ttttccctat ttatccacag gactttccag aactcgctaa gtgtaaacga
    46081 tcctgccgcg aggcgggcac gatttacgcc gcatattgga aaatttaatg accatagaca
    46141 aaaattggct taatcgatct aataaagatc caggacgatc cttgcgcttt acccatcagc
    46201 ccgtataatc ctccacccgg cgcgccatgc tggtttccac tggtgtgagg tcgtacattt
    46261 tccctgcgaa aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggtgtg
    46321 tacaattatt acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt
    46381 tttcggcgga tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg
    46441 aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt
    46501 atggctacta aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt
    46561 ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga
    46621 gttacgcttg ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg
    46681 cacgccgcaa attaccattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct
    46741 tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac
    46801 gcgtgaaagc ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc
    46861 gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg
    46921 gcgccgccac tgtctcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc
    46981 ctgattagtc cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga
    47041 attgaggcat tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta
    47101 ttaaaatgcc accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat
    47161 accagagaac actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg
    47221 tgtctttcat gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac
    47281 agaccacgca gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca
    47341 gtggccaggg gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc
    47401 gtggtggtga tgttgagcaa gctctgctgc ctgcttaccc gaaagagctg aactctaccc
    47461 agccgttcca gctgttggaa acttcaccgc agtttattta tcaggcacag agcggtctga
    47521 ccggtcgtga tggcccggat aacccggcta acggcccgcg tccgctgtat aacgttgaaa
    47581 aagacgctta tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg
    47641 acgcggcagg caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca
    47701 acgtcaacta caacgtgcag aacgctggcg agaaaccgct ggaaatctcc tcgtttggtc
    47761 agttgaagca atccatcact ctgccaccgc atctcgatac cggaagcagc aacttcgcac
    47821 tgcacacctt ccgtggcgcg gcgtactcca cgcctgacga gaagtatgag aaatacaagt
    47881 tcgataccat tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcga
    47941 tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct
    48001 ataccgctaa tctgggtaac ggcatcgtcg ctatcggcta taaatctcag ccggtactgg
    48061 ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg
    48121 acaaaatggc agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt
    48181 tcatctctca gccgctgttc aaactgctga aatggatcca tagctttgtg ggtaactggg
    48241 gcttctccat tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag
    48301 cgcagtacac ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg
    48361 agcgtctggg cgatgacaaa cagcgtatca gtcaggaaat gatggcgctg tacaaagctg
    48421 agaaggttaa cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg
    48481 cgttgtacta catgctgatg ggttccgttg aactgcgtca ggcaccgttt gcactgtgga
    48541 tccacgacct gtcggcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga
    48601 tgttcttcat tcagaagatg tcgccgacca ctgtgaccga cccgatgcag cagaagatca
    48661 tgacctttat gccggtcatc ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtac
    48721 tgtactatat cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc
    48781 tggaaaaacg tggcctgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc
    48841 taaaataagg gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccgcg
    48901 catccgccac acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg
    48961 ccacacctcc gggacgtggc ggcgttggca tcctgcgcat ctccggcttc aaagcccgtg
    49021 aagttgccga aaccgtgctg ggtaaactgc ctaagccgcg ctacgccgat tatcttccgt
    49081 ttaaagacgc cgacggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga
    49141 actcgttcac cggcgaagat gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg
    49201 acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg gattgctcgc cctggtgagt
    49261 tttccgaacg cgcgtttctt aacgataaac ttgacttagc ccaggccgag gcgattgccg
    49321 atcttatcga cgccagttcg gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg
    49381 cattctccgc acgggttaat catctggtag aagccctcac ccacttgcgc atttacgtcg
    49441 aagcggcaat tgatttccct gatgaagaga tcgatttcct ctccgacggc aaaattgaag
    49501 cccagcttaa tgacgtgatg gccgatctcg atgcagtgcg tgctgaagca cgtcagggta
    49561 gtttgttgcg cgaagggatg aaagtagtga ttgccggacg tcctaatgcc ggtaaatcga
    49621 gcctgttaaa cgcgctggcg ggccgtgaag cggcaatcgt aaccgatatc gccggaacca
    49681 cgcgtgacgt gctgcgtgag catatccaca ttgacggaat gccgctgcat atcatcgaca
    49741 ctgccgggct acgtgaggcc agtgacgaag tggaacgtat tggtatcgag cgcgcgtggc
    49801 aggaaattga acaggccgac cgcgtgctgt ttatggtcga tggcaccacg acagacgccg
    49861 ttgatccggc agagatctgg ccggaattta tcgcccgtct gcccgcgaaa ctgccgatca
    49921 ccgtggtacg caataaagcc gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg
    49981 gtcacgcgtt aattcgtctc tcggcaagga ctggtgaagg cgtggacgtg ctgcgtaacc
    50041 atctcaaaca gagcatgggc tttgacacca atatggaagg cggcttcctg gcgcgccgtc
    50101 gccacttaca ggcgctggaa caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt
    50161 tgggagcctg ggcaggtgaa ctgctggcgg aagagttgcg cctggcacag cagaacttaa
    50221 gcgaaatcac cggggaattt acttcagacg acctgctggg gcggattttc tccagcttct
    50281 gtattggtaa gtaaccgcgc ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt
    50341 cattgttagc actcctgtta tgcctcaacc cttttttaaa cattaaa
//
LOCUS       DADLTI010000008        49515 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLTI010000008 DADLTI010000000
VERSION     DADLTI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.008"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JN755_000475"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JN755_000475"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7379383.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN755_000476"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN755_000476"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7379384.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN755_000477"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN755_000477"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7379385.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JN755_000478"
     CDS             complement(3025..3480)
                     /locus_tag="JN755_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7379386.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN755_000479"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN755_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7379387.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN755_000480"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN755_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7379388.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JN755_000481"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JN755_000481"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7379389.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7618..10152
                     /gene="mrcB"
                     /locus_tag="JN755_000482"
     CDS             7618..10152
                     /gene="mrcB"
                     /locus_tag="JN755_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7379390.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10372..12615
                     /gene="fhuA"
                     /locus_tag="JN755_000483"
     CDS             10372..12615
                     /gene="fhuA"
                     /locus_tag="JN755_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7379391.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12666..13463
                     /gene="fhuC"
                     /locus_tag="JN755_000484"
     CDS             12666..13463
                     /gene="fhuC"
                     /locus_tag="JN755_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7379392.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13463..14353
                     /gene="fhuD"
                     /locus_tag="JN755_000485"
     CDS             13463..14353
                     /gene="fhuD"
                     /locus_tag="JN755_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7379393.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14350..16332
                     /gene="fhuB"
                     /locus_tag="JN755_000486"
     CDS             14350..16332
                     /gene="fhuB"
                     /locus_tag="JN755_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7379394.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16367..17647)
                     /gene="hemL"
                     /locus_tag="JN755_000487"
     CDS             complement(16367..17647)
                     /gene="hemL"
                     /locus_tag="JN755_000487"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7379395.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17872..19293
                     /gene="clcA"
                     /locus_tag="JN755_000488"
     CDS             17872..19293
                     /gene="clcA"
                     /locus_tag="JN755_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7379396.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19317..19382
                     /locus_tag="JN755_000489"
     CDS             19317..19382
                     /locus_tag="JN755_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379397.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19375..19719
                     /gene="erpA"
                     /locus_tag="JN755_000490"
     CDS             19375..19719
                     /gene="erpA"
                     /locus_tag="JN755_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7379398.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19766..20389)
                     /locus_tag="JN755_000491"
     CDS             complement(19766..20389)
                     /locus_tag="JN755_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7379399.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20427..21227)
                     /gene="btuF"
                     /locus_tag="JN755_000492"
     CDS             complement(20427..21227)
                     /gene="btuF"
                     /locus_tag="JN755_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7379400.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21220..21918)
                     /gene="mtnN"
                     /locus_tag="JN755_000493"
     CDS             complement(21220..21918)
                     /gene="mtnN"
                     /locus_tag="JN755_000493"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7379401.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22002..23519
                     /gene="dgt"
                     /locus_tag="JN755_000494"
     CDS             22002..23519
                     /gene="dgt"
                     /locus_tag="JN755_000494"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7379402.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23649..25073
                     /gene="degP"
                     /locus_tag="JN755_000495"
     CDS             23649..25073
                     /gene="degP"
                     /locus_tag="JN755_000495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7379403.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25228..26384
                     /locus_tag="JN755_000496"
                     /pseudo
     CDS             25228..26384
                     /locus_tag="JN755_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26473..26859)
                     /locus_tag="JN755_000497"
     CDS             complement(26473..26859)
                     /locus_tag="JN755_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7379404.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27173..27997)
                     /gene="dapD"
                     /locus_tag="JN755_000498"
     CDS             complement(27173..27997)
                     /gene="dapD"
                     /locus_tag="JN755_000498"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7379405.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28028..30700)
                     /gene="glnD"
                     /locus_tag="JN755_000499"
     CDS             complement(28028..30700)
                     /gene="glnD"
                     /locus_tag="JN755_000499"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7379406.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30762..31556)
                     /gene="map"
                     /locus_tag="JN755_000500"
     CDS             complement(30762..31556)
                     /gene="map"
                     /locus_tag="JN755_000500"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7379407.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31924..32649
                     /gene="rpsB"
                     /locus_tag="JN755_000501"
     CDS             31924..32649
                     /gene="rpsB"
                     /locus_tag="JN755_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7379408.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32907..33758
                     /gene="tsf"
                     /locus_tag="JN755_000502"
     CDS             32907..33758
                     /gene="tsf"
                     /locus_tag="JN755_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7379409.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33905..34630
                     /gene="pyrH"
                     /locus_tag="JN755_000503"
     CDS             33905..34630
                     /gene="pyrH"
                     /locus_tag="JN755_000503"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7379410.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34922..35479
                     /gene="frr"
                     /locus_tag="JN755_000504"
     CDS             34922..35479
                     /gene="frr"
                     /locus_tag="JN755_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7379411.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35571..36767
                     /gene="ispC"
                     /locus_tag="JN755_000505"
     CDS             35571..36767
                     /gene="ispC"
                     /locus_tag="JN755_000505"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7379412.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36956..37714
                     /gene="ispU"
                     /locus_tag="JN755_000506"
                     /gene_synonym="uppS"
     CDS             36956..37714
                     /gene="ispU"
                     /locus_tag="JN755_000506"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7379413.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37727..38584
                     /gene="cdsA"
                     /locus_tag="JN755_000507"
     CDS             37727..38584
                     /gene="cdsA"
                     /locus_tag="JN755_000507"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7379414.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38596..39948
                     /gene="rseP"
                     /locus_tag="JN755_000508"
     CDS             38596..39948
                     /gene="rseP"
                     /locus_tag="JN755_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7379415.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39978..42410
                     /gene="bamA"
                     /locus_tag="JN755_000509"
     CDS             39978..42410
                     /gene="bamA"
                     /locus_tag="JN755_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7379416.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGEQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42532..43017
                     /gene="skp"
                     /locus_tag="JN755_000510"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42532..43017
                     /gene="skp"
                     /locus_tag="JN755_000510"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7379417.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43021..44046
                     /gene="lpxD"
                     /locus_tag="JN755_000511"
     CDS             43021..44046
                     /gene="lpxD"
                     /locus_tag="JN755_000511"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7379418.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44151..44606
                     /gene="fabZ"
                     /locus_tag="JN755_000512"
     CDS             44151..44606
                     /gene="fabZ"
                     /locus_tag="JN755_000512"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7379419.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44610..45398
                     /gene="lpxA"
                     /locus_tag="JN755_000513"
     CDS             44610..45398
                     /gene="lpxA"
                     /locus_tag="JN755_000513"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7379420.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45398..46546
                     /gene="lpxB"
                     /locus_tag="JN755_000514"
     CDS             45398..46546
                     /gene="lpxB"
                     /locus_tag="JN755_000514"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7379421.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46543..47139
                     /gene="rnhB"
                     /locus_tag="JN755_000515"
     CDS             46543..47139
                     /gene="rnhB"
                     /locus_tag="JN755_000515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7379422.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47176..>49515
                     /gene="dnaE"
                     /locus_tag="JN755_000516"
     CDS             47176..>49515
                     /gene="dnaE"
                     /locus_tag="JN755_000516"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7379423.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPA"
BASE COUNT        11594 a        12214 c        13762 g        11945 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc accctaaccc
     7501 tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc ttcttctgtc
     7561 ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg
     7621 gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa
     7681 aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga tgactatgag
     7741 gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa gcctcgtggc
     7801 aaacgcggct ggctatggct actgctaaaa ctggctatcg tttttgccgt gctgatcgcc
     7861 atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa
     7921 ctgcccgcgg cagtttatgg ccgaatggtc aaccttgaac cagatatgac catcagcaag
     7981 aacgaaatgg tgaagctgct ggaggcaaca cagtatcgtc aggtgtcgaa aatgacccgt
     8041 cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc gtttgatttc
     8101 ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggtga tcatctggcg
     8161 gccatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga tccgcgtctg
     8221 atcaccatga tctcctcgcc aaacggtgag cagcgtttgt ttgtgccgcg cagtggtttc
     8281 cctgatttgc tggtggatac tttgctggcg acagaagacc gtcattttta cgagcatgat
     8341 ggaatcagtc tctactccat tgggcgtgcg gtgctggcaa acctgaccgc aggtcgcacg
     8401 gtgcagggtg cgagtacgct gacgcaacag ctggtgaaaa acctgttcct ctccagcgag
     8461 cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga cgcgcgttac
     8521 agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca gagcggcgac
     8581 aacgaaatcc gcggcttccc gctggcaagc ttgtattact ttggtcgccc ggtagaagag
     8641 ctaagcctcg accagcaggc gctgttagtc ggtatggtga aaggggcgtc catctacaac
     8701 ccgtggcgta acccgaaact ggcgctggag cgacgtaatc tggtgctgcg tctgctgcaa
     8761 cagcaacaga ttattgatca agaactctat gacatgttga gtgcccgtcc gctggggggt
     8821 cagccgcgtg gtggggtgat ctctcctcag ccagccttta tgcagttggt gcgtcaggag
     8881 ttgcaggcaa aactgggcga taaggtaaaa gatctctccg gcgtgaagat cttcactacc
     8941 tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat tccggcactg
     9001 aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga ccgctttagt
     9061 ggtgaagttc gtgcgatggt cggaggttct gagccgcagt ttgcgggcta caaccgtgcg
     9121 atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct gacggcttta
     9181 agccagccga aaatctatcg cctgaacacg tggattgccg atgcgccaat cgcgctgcgt
     9241 cagccgaatg gccaggtctg gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc
     9301 agagtgatgc tggtggatgc gttgacacgt tcgatgaacg tgccgacggt aaatctgggg
     9361 atggcgctgg ggctgcctgc ggttacggag acctggatca aactgggcgt accgaaagat
     9421 cagttgcatc cggttccggc aatgctgctg ggggcgttga acttaacgcc aatcgaagtg
     9481 gcgcaggcat tccagaccat cgccagcggt ggtaaccgtg caccgctttc tgcactgcgt
     9541 tcggtaatcg cggaagatgg taaggtgctg tatcagagct tcccgcaggc agaacgcgct
     9601 gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt acaacgtggt
     9661 acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa aacagggact
     9721 accaacaata acgtagatac ctggtttgca ggcattgacg gcagcacggt gaccatcacc
     9781 tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg ggcaatgtcg
     9841 atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt tccgccagaa
     9901 gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg tggcatgcgt
     9961 gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga gatgcagcag
    10021 cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc gcaacagcaa
    10081 cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa ggatatgttt
    10141 ggtagtaatt aacatctaag cgtgaaatac cggatggcga gttgccatcc ggtaaaataa
    10201 catcccatct aagatattaa ccctttcttt tcatctggtt gtttattaac ccttcaggaa
    10261 cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc ttatctttat
    10321 aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc aatggcgcgt
    10381 tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt agccacagcg
    10441 gttagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga cactatcacc
    10501 gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg
    10561 cggcagtcag ctaccggcac taaaaccgat acgccgattc aaaaagtgcc acagtctatt
    10621 tctgttgtga ccgccgaaga gatggcgctg catcagccga agtcggtaaa agaagcgctt
    10681 agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta tgaccacctg
    10741 atcattcgcg gttttgcggc agaaggccaa agccaaaata actatctgaa tggcctgaag
    10801 ttgcagggca acttctataa cgatgcggtc attgacccgt atatgctgga acgcgctgaa
    10861 attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg tctgttgaat
    10921 atggtcagca agcgtccgac caccgaaccg ctgaaagaag ttcagtttaa agccggtact
    10981 gacagcctgt tccagactgg ttttgacttt agcgatgcgt tggatgatga cggtgtttac
    11041 tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg gtcagaagag
    11101 cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac caatttcacc
    11161 ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt gccgaaagag
    11221 ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa tgaaggggcg
    11281 aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga tcacgaattt
    11341 aacgacacct ttactgtgcg tcagaacctg cgctttggtg aaaacaaaac ctcgcaaaac
    11401 agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa acagtgtgcg
    11461 gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt tgatgatgag
    11521 aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac tggcgatatc
    11581 gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat caacgcctgg
    11641 tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa tcccgatttc
    11701 gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa taaacagaaa
    11761 caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt caccctaggc
    11821 ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgctgggac gaccgataaa
    11881 cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga taatggtgta
    11941 acaccttact tcagctatag cgaatcgttt gaaccttctt cgcaagttgg gaaggatggt
    12001 aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata tgtaccggaa
    12061 gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa caacctgatg
    12121 gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc acgtggcgta
    12181 gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc ttatacttac
    12241 accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc acaggtgcca
    12301 aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct ttcaggtttg
    12361 acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc taactccttt
    12421 aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc gcgagtcggc
    12481 atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga atacgtcgcc
    12541 agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca
    12601 acctttcgtt tctaatttct cttttggggc acggatttcc gtgcccattt cacaagttgg
    12661 ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt aatatctcct
    12721 ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct gccgggaaag
    12781 tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa atgcttggtc
    12841 gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc ccagccgctg gaaagctgga
    12901 gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct ccggcagaag
    12961 ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc gcgctggggc
    13021 gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat ctcgctggtt ggcttaaaac
    13081 cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcagcgg gcgtggatcg
    13141 ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgact tcggcgctgg
    13201 atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc
    13261 tgacggtcat tgccgtgttg cacgatatca atatggcggc acgctactgt gattatctgg
    13321 tcgccctgcg cagtggtgaa atgattgctc agggaacgcc tgcggaaatt atgcgcggcg
    13381 aaaccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg gcgggtgctg
    13441 cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt
    13501 aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg cggcggctat
    13561 tgaccccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc tggcgctcgg
    13621 catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca gcgaaccacc
    13681 attgccggac tcagtgattg acgtcggttt gcgtacagaa cctaaccttg aactgctgac
    13741 cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcat cagaaatgct
    13801 ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac ggcaaacatc cgttggcgat
    13861 ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac ctgcaaagcg cagcggaaac
    13921 gcatttagcg caatacgaag actttatccg cagcatgaaa ccccgctttg tgaagcgtgg
    13981 tgcacgcccg ttactgctga cgacgcttat cgatccgcgc catatgctgg tcttcggtcc
    14041 aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct ggcaagggga
    14101 aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcagcgt ataaagacgt
    14161 tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa tggcaacccc
    14221 gctgtggcag gccatgccgt ttgtccgtac agggcgcttt cagcgcgtac ccgcagtctg
    14281 gttttatggt gcaacgcttt cggcaatgca ctttgtgcgt gttctggata acgccatcgg
    14341 aggtaaagcg tgagtaaacg aattgcgctt ttcccggcgt tattgctggc gctgttagtg
    14401 attgtcgcta cggcgctcac ctggatgaac ttctcacagg cgctgccgcg cagtcagtgg
    14461 gcgcaggccg cctggtcgcc ggatattgac gtcatcgagc agatgatttt tcactacagc
    14521 ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcgg gcctggggct ggtgggcgtg
    14581 ctgtttcagc aagtactgcg taacccgctg gcggaaccga cgacgttggg cgttgcaaca
    14641 ggcgcgcaac tggggattac cgtcaccacg ctctgggcga tccctagcgc gatggcgagc
    14701 cagtttgctg cgctggcagg ggcttgcgtt gttggcttaa ttgtctttgg cgtcgcgtgg
    14761 gggaaacggc tgtcgccggt aacgctgatt ctcgcggggt tggtagtgag cctttattgc
    14821 ggcgcaatca atcagttact ggttatcttc catcatgacc agttgcaaag catgtttctg
    14881 tggagcactg gaacgctgac gcaaaccgac tggggcggtg ttgagcgttt atggccgcag
    14941 ttgctgggcg gtgtgatgct gacgttgctg ctacttcgtc cgttaaccct gatggggctt
    15001 gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc ttgcgcgtct ggcggcgctg
    15061 tcgctggcga ttgtcatcag tgcgctgctg gtgaacgctg tggggattat cggctttatc
    15121 gggttgttcg cgccgctgct ggcaaaaatg ctgggggcgc ggcgtctgct gccacgactg
    15181 atgctggcgt cgttgattgg tgcgctgatc ctctggcttt ccgatcaaat catcctctgg
    15241 ctgactcgcg tgtggatgga agtgtccacc ggttcggtca ctgcgttgat cggtgcgccg
    15301 ctgctactgt ggctgctgcc gcgtttacgc agcattagcg cgccggatat gaaggttaac
    15361 gatcgtgtcg cgactgaacg ccaacatgtg ctggcgtttg ccctcgcggg cggcgtgctg
    15421 ttgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg
    15481 gcgagcgggg cgttgctcga ggatttaatg ccctggcgct ggccgcgaat tatggcggcg
    15541 ctgtttgcgg gcgtcatgct ggcggtagcg ggctgtatta ttcagcgact gaccggcaac
    15601 ccgatggcaa gcccggaagt gctggggatt agctccggcg cggcgtttgg tgtggtgtta
    15661 atgctgtttc tggtaccggg taatgccttt ggctggttgt tacccgcagg cagtctcggg
    15721 gcggcggtga tgctgttgat cattatgatc gccgccggac gcggtggatt ttccccacac
    15781 cgcatgttac tggcggggat ggcgttaagt accgcgttca ccatgctttt gatgatgttg
    15841 caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct ggatttccgg ttcgacctac
    15901 aacgcgaccg atgctcaggt ctggcgcacc ggaattgtga tggtgatttt gctggcgatt
    15961 actccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac cgcgcgggcg
    16021 gtagggatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg
    16081 gcgaccgcga cgatgactat tggaccgttg agttttgttg gtttaatggc accgcatatt
    16141 gcgcggatga tgggctttcg acggacgatg ccacatatcg taatttcggc gctggtgggt
    16201 ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tgctgtttcc attccagatc
    16261 ccggcggggc tgctgtcgac ctttatcggt gcgccatatt ttatctattt gctgagaaag
    16321 cagagccgtt aatagaatgc ctcgcccggt gggcgaggca aaattatcac aacttcgcga
    16381 acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc gccacagaca
    16441 taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcatccagc atcatatgga
    16501 agaaacgctt aaaacgctcg acgtcacagg ccatcacatc ctgatagcac gtcacggact
    16561 cggcgtcggt aaagaagata ccgaacatac caccgacgtg gttaacgacc agcggaattc
    16621 ccgcttcttc tgccgcttcc agcagacctt ctgccagacg tgttgtcagc tcatccagcg
    16681 tttcgtgaat gcccggctgc gcgacttcat tcagacaggc gaaacccgct gccatcgcaa
    16741 tcgggttacc ggaaagcgta cccgcctgat agaccggacc tgtaggggcc agcgcatcca
    16801 ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg atttttccga
    16861 ggcaggttaa atctggcact acgccgaaat aatcctgtgc gccagctagc gctacgcgga
    16921 aaccggtcat cacttcatcg atgatcagca acgcgccaaa ttcgtcgcac agcgcacgca
    16981 gacctggcag gaactctggc agcggcggaa cacagttcat attgcctgcc accggctcga
    17041 cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca gaggccagat
    17101 cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacgccc ggcgagtttg
    17161 gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt
    17221 ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg gccaagcgga
    17281 tggcgctcat cgtcgcctcg gtgccggagt tcaccatgcg caccatatct atggtcggga
    17341 ccagttcggt catcagttgc gccattttca cttccatttc ggtcggtgca ccaaagctta
    17401 aaccacgctc ggcggcttca atcacggcat tgcggatcgc cggatggtta tggcccagca
    17461 ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca tcgtacagat
    17521 aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa
    17581 caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga ttttcagact
    17641 tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct atgttattca
    17701 taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt
    17761 agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt tcatggaacg
    17821 tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg gatgaaaact
    17881 gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca actgattcgc
    17941 caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc agtcgtcggc
    18001 acgcttatcg ggctggctgc ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa
    18061 cgtatggggg cgctggtaca tactgctgat aattatccgc tactgttaac cgtcgctttt
    18121 ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata cgcgcccgaa
    18181 gcagggggat cggggatccc ggaaattgaa ggggcgctgg aagatcaacg tcctgttcgc
    18241 tggtggcgtg tattgccggt gaaattcttt ggcgggctgg ggacactcgg cggaggcatg
    18301 gtgctagggc gcgaaggacc aaccgtgcag atcggcggca atatcggtcg catggtgctt
    18361 gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac cggtgctgct
    18421 gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat tatcgaagag
    18481 atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat tggcgtcatt
    18541 atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga cgtcggtaaa
    18601 ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat tatttttggc
    18661 attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct gcaccgtgtg
    18721 cacggcggca atattaccaa atgggtactg atgggggggg cgattggcgg tctgtgtgga
    18781 ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct
    18841 accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc
    18901 accttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat gctggcgctg
    18961 ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agctgtttcc gcaatatcac
    19021 ctggaggcgg ggacgtttgc tattgccgga atgggggcat tactggcggc atctattcgc
    19081 gcgccgttaa cggggatcat tctggttctg gagatgaccg ataactacca gctcattttg
    19141 ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg cgggaaacct
    19201 ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca actggcgcga
    19261 agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat tagataatgg
    19321 cgattattat tgggttagaa tttgcccgat tgccgatgtc gtttggagca aaatatgagt
    19381 gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt taaaagcctg
    19441 atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc
    19501 agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga tatgaccatc
    19561 gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt
    19621 tccgttgact acaccgaagg actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg
    19681 aaaagcacct gcggttgcgg ttcttccttt agcatctaat ctgttgttca tgtgccggat
    19741 aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca aacgtcggta
    19801 gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc atgcccatca
    19861 tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg acaataccgc
    19921 cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt tcgcgggcca
    19981 gtacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa accgcgatta
    20041 acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac accgccagac
    20101 caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg cgcaccagca
    20161 cgatggtcag catgctggtg accatggcaa cgaccagatc ggtgggatct ttcacccaaa
    20221 ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gtcgcctact gcggtaacca
    20281 cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccgg
    20341 agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc
    20401 cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt acagagctgt
    20461 tgtgctgcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata
    20521 acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg
    20581 cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct aacttgcggc
    20641 cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttc
    20701 tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt
    20761 tttttaggtt tatcagcata ttgcgctttc aattgcacgt actgatccag cagggattgc
    20821 gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg taacgcattg
    20881 gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc
    20941 tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc
    21001 gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct
    21061 tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca
    21121 agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc
    21181 gcgagaaaag acagggcgac cagcgccctg aacagtgatt tagccatgtg ccagtttctg
    21241 caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga actcatcgaa
    21301 gctaagatga gactgttgat cggccacgtc ggagatggcg cgtacgacaa caaacgggac
    21361 gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg
    21421 tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct
    21481 aacaatcagg ccacgtacag cgttaagatt cagttcggca atgcaggcct cagcggcagc
    21541 gatcagttta tcgtcagctt taaagcctgt cggacagcct ggtaactgac cgtattcata
    21601 accaaatgcc gtgacatccg cgtcgtgata acgcgcttcg tccgagacaa cgatatcgcc
    21661 cactttcagc gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt
    21721 gcagtgttcc aacagcagag tggcacccag cgccgcagcg actttaccga tgcccgattt
    21781 cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac cgccgagact
    21841 gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc
    21901 aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc atagtttacc
    21961 atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc
    22021 gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg
    22081 agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc
    22141 gtctgcaaca aaagacccag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc
    22201 ttacccactc gatggaagtc cagcaggtgg gacgctacat cgccaaagaa attttaagcc
    22261 gtctgaaaga gcttaaattg ctggaagcat acggcctgga tgaactgacc ggcccctttg
    22321 aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg tttggtcatt
    22381 ttggcgaagc agcgataaat gactggtttc gccaacgttt gcacccggaa gatgccgaaa
    22441 gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg gacggggaag
    22501 aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt atgtcatttt gaggggaatg
    22561 cacaaggcat tcgtctggtg catacattga tgcggatgaa tctcacttgg gcacaggttg
    22621 gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc
    22681 actatttaat gaaaaagccg ggttattatc tttctgaaga agcctatatt gcccggttgc
    22741 gtaaagaact taatttggcg ctttacagtc gttttccatt aacgtggatt atggaagctg
    22801 ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa agaatattta
    22861 ccgttgagca gctttatcat catttgcacg aagcgtgggg ccagcatgag aaaggttcgc
    22921 tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca
    22981 gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaacaaa ctggtaccct
    23041 acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg tttaatcatg
    23101 cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa aatgtcgctg
    23161 taaaacatgt atttagccat ccggatgtcg agcagcttga attgcagggc tatcgggtca
    23221 ttagcggatt attagagatt tatcgtcctt tattaagcct gtcgttatca gactttactg
    23281 aactggtaga aaaagaacgg gtgaaacgtt tccctattga gtcgcgctta ttccacaaac
    23341 tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg tcagattctc
    23401 ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat tatatcagcg
    23461 gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta gaacaataac
    23521 caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt agttcggaac
    23581 ttcgggctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt taatcgagac
    23641 tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt ttaggtttgg
    23701 cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca gcccagcaga
    23761 tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc attaacgtag
    23821 aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg
    23881 atgattctcc gttctgccag gaaggttctc cgttccagag ctctccgttc tgccaggggg
    23941 gccagggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt tccggcgtca
    24001 tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg
    24061 tgattaaagt ccagctgagc gatggtcgta agttcgacgc gaagatggtt ggcaaagatc
    24121 cgcgctctga tatcgcgctg atccaaatcc agaacccgaa aaaactgacc gcaattaaga
    24181 tggcggattc tgatgcactg cgcgtgggtg attacaccgt agcgattggt aacccgtttg
    24241 gtctgggcga gacggtaact tccgggattg tctctgcgct ggggcgtagc ggcctgaatg
    24301 ctgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccgtggt aactccggtg
    24361 gtgcgctggt taacctgaac ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg
    24421 acggcggcaa catcggtatc ggttttgcta tcccgagtaa catggtgaaa aacctgacct
    24481 cgcagatggt ggaatacggg caggtgaaac gcggtgagct gggtattatg gggactgagc
    24541 tgaattccga actggcgaaa gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc
    24601 aggttctgcc taattcctcg gctgcaaaag cgggcattaa agcgggtgat gtgatcacct
    24661 cactgaacgg taagccgatc agcagctttg ccgcactgcg tgctcaggtg ggtactatgc
    24721 cggtgggcag caaactgacc ctgggcttac tgcgcgacgg taagcaggtt aacgtgaacc
    24781 tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc aacggcattg
    24841 aaggcgctga gatgagcaac aaaggtaaag atcagggcgt ggtagtgaac aacgtgaaga
    24901 cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt ggtgcgaacc
    24961 agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc
    25021 tggcactgaa cattcagcgc ggcgacagca ccatctacct gttaatgcag taatctccct
    25081 caaccccttc ctgaatacgg gaaggggttc tccttacaat ctgtgaactt cgccacaact
    25141 ccatacatct tcatcatcct ttgggcattt gcacaatgcc gtacgttacg tacttcctta
    25201 tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac caaaatggcg
    25261 caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt aatggatgcc
    25321 cgcgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca
    25381 ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtagc acgtcatctg
    25441 cacggtgtgc ggcaggggat taatctaccg ttacggctgg aaggtgaaat tgtcggcgta
    25501 attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt ctgcatgacg
    25561 gctgaaatga tgctggaaca gtcgcggttg atgcacttgt tggcgcagga tagccgtttg
    25621 cgggaagaac tggtgatgaa cctgattcag gcagaggaga atactcccgc acttctgaat
    25681 gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt gttgaggtcg
    25741 acagcggtca gcttggcgtg gacagcgcaa tggcggagtt acaacaactg caaaacgcgc
    25801 tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa atggtggtgt
    25861 tgaaaccggc gttgaactct tttgggcgct gggatgcaga agatcatcgt aagcgagttg
    25921 aacaactgat tacccgcatg aaagagtacg gccagctgcg ttttcgcgtt tcactgggca
    25981 actattttac cggtcctagc agtattgccc gatcctatcg tacggcgaaa acgacgatgg
    26041 tggtgggtaa acagcggatg ccagaaagtc gctgctattt ttatcaggat ctgatgttac
    26101 ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg cgaccgctgg
    26161 cgcggctgaa agcgatggac aataacggct tgctgcgacg aacgctggcg gcgtggtttc
    26221 gccacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt tattcatcgt aataccctgg
    26281 agtatcggct taatcgtata tcggaactga ccgggcttga tttgggcaat tttgatgaca
    26341 ggttgctgct gtatgtggcg ttacaactgg atgaagagcg gtagattatg cgttaaggtt
    26401 gaggcggcgc tggctcattc ccctcagccc gaaggggaga ggggaatgcc cgagttaacc
    26461 gtcaattcgg gattatttac gcgttagttt ttccagatcg gcttcaatct cgctgatctt
    26521 attggttacg actgactcca ggtgacgtaa gtcgtcgagg atcttacgct taagatcaac
    26581 ttcgctgcgg tcacgctgac agatttgatc aagctcatca atgatatacc gtagattcgg
    26641 gctgatttcc tggacttctt tataaccctg acccacacca tcagcgacga ccgttttacg
    26701 ctgacgcgga tatttaaact taacgctctt ggcgaaaaac tcgcctttgt ctttctggaa
    26761 atagattttc agaatatcgt tgttggcttc ctgccggagg ctgtaacgat caatttcttc
    26821 aggattggta atacccagac ttttcagatt gtcgtacata gcgttacctc aaaatgagtc
    26881 agtaaattgt gcttatttta gcatttggct tggcccgccc ggctcgactg tgatcgcaga
    26941 aagccttttt cttccagact tctatcatgg cgtaaaaatc aaaaaattac ctgctttatt
    27001 gtggcgatat tccatgggat ggattagcac atttatcttc gttttgcgaa ttacatgaca
    27061 gtccgaaaca agatgaaaaa gtatttaaaa aagggggagg aagaatgagt aggacgaatt
    27121 attctgagcc agaaagcata cgggcagcat catgctgccc gtatatgtac tcttaatcga
    27181 tggtacgcag cagttcgtta atgccgactt tgccgcgagt tttcgcgtca actttcttaa
    27241 cgataaccgc acagtagagg ctgtatttgc catcttttga cggcagatta cctgaaacaa
    27301 ccacagaccc cgccggaacg cgaccgtagt ggatttcgcc ggtttcacgg tcgtaaatac
    27361 gggtgctctg accaatgtat acgcccatag agatgacgga accttcttcg acaatcaccc
    27421 cttcaaccac ttcagagcgt gcgccaatga agcagttatc ttcaataatg gtcgggttag
    27481 cctgcagcgg ctccagcacg ccgccgatgc ccacgccacc ggaaaggtgg acgtttttac
    27541 caatctgcgc acaagaaccg acggtcgccc aggtatcaac catggtgcct tcatcaacat
    27601 atgcgccgat gttaacgtaa gacggcatca gcacggtgtt acgggcaata aacgcaccct
    27661 gacgtaccgc cgctggtggc acaacgcgga agccttcttt ctggaaacgt gcttcgtcgt
    27721 agtcggcgaa tttcatcggc actttgtcga agtagcggct ttctgcccct tcgatcacct
    27781 gattatcatt aatacggaaa gagagcagca ccgctttttt caaccactga tgcgtcaccc
    27841 actgaccgtc aattttttcc gctacacgca gtgcgccgga atccagcagg gcgatcacct
    27901 gatttaccgc ttcgcgggta acggtgtctg cattggctgg cgtgatctcg gcacggcgtt
    27961 caaaagcggt ttcaataatg ttctgtaact gctgcattgt taaactcttt tcatatcagt
    28021 aaacacatca ccctttatcg tttggattga gggcctctgt caaccgctga tgcacttcct
    28081 gctgcaactc gttattaagc gcacgccggt cagcggtggc aattatgaat aaatcttcta
    28141 ctcgctcgcc aatggttgta attcgggcac catgaagcga aattcccaga tcggcaaaaa
    28201 ttttcccgac tcgcgccagc agtccaggtt ggtcgagggc gatcagttcg aggaacgatt
    28261 tgcggtcggt atgggtcggc aaaaacgtta cttcggtttc aacagtaaaa tggcgtaatt
    28321 tggcgggttg gcgacggggc tgcggtggct gccagctact ttgcgtcagc acctgctcca
    28381 gaccaaaccg aataacctca tggcgatctg cggacagcgg gctgccatcg ggttccagca
    28441 cgataaaggt atccatcgcc ataccgtcgc gagtggtgaa aatttgtgcg tcgtgaacac
    28501 ttaaattgcg gcggtctaat tcggcacaga cggcggcaaa cagatagggg cggtccgggc
    28561 tccagataaa aatctcggtg cctccacgcg tagcctgcgg gctaagcaat accagcggtt
    28621 tgcttaaatc atgctgtaat aaatggcggg catgccaggc cagttgattt gggctatggc
    28681 ggacaaaata gttagcacga cagcgtgacc aaatttggtg cagcgcctct tcgtcgatgt
    28741 tatccatgcg cagtagtgcc agtgcctgga gttggtgatg gcgaacccgt tcgcgcatat
    28801 ccggcgtgtt ttgcattccg cgtcgtagct gcttttcggt ggcaaaatag agttcgcgca
    28861 acagactttg cttccagcta ttccacagcg tttcgttggt ggcgcaaatg tcagccactg
    28921 tcaggcatac cagatagcgc agacgatttt ccgtttgaac ttcttcggca aactgcttga
    28981 tgacttcagg gtcctgaata tcgcggcgtt gtgctgtcac cgacatcagc aagtgctggc
    29041 gaaccagcca ggcaaccagc tgtgtttcgc gtgagttcag cccgtggagt tctgcaaaat
    29101 gcactacatc ctgagcaccg agaatggagt ggtcgccgcc gcgtcctttg gcgatatcgt
    29161 gaaacagcgc ggcgatgaaa atcagctcag ttgacggcag gcgcggccag acgtccacac
    29221 acaacggatg gcgctggcgc gtttcttcac tggcaaaact ctccagtttc agcatcacgc
    29281 ggatagtatg ttcatccacc gtgtaggcgt ggaacagatc aaactgcatc tgcccgacga
    29341 tatgcgacca ttgcggcata tacgcgccga gcacgctatg gcgatgcatt ggcaatagcc
    29401 cgcgccgcac cgctccgggg tgacgcagaa tgctcaaaaa cagttttcgt gcttgcggaa
    29461 tattacacag cggttgttgc agatggcgac gggcatggcg taactggcgc agcgtggtgg
    29521 agtaaatacc ggtgatcgca ctgttgcgca ccatggtgta gaacatacgc aagatggctt
    29581 ccggctggcg cataaatagt gtttcatcac gcaggtcgat tagcgtaccg cgtagctgaa
    29641 actcatcgtc gagtggacgt ggtttttcgt cggcgggaag ggcgaggatg gcttcatcga
    29701 acagttgcag cagcatctgg ttgagttcac tgacgcggcg tgtaacgcgg aagtaatcct
    29761 tcatcatccg ctcgaccggt tcgttgcctt caccactgta attcagacgc tgagcgacgc
    29821 taagttggcg atcgaataac agacgattat cgtaacggct gacgaccaga tgcagggcaa
    29881 agcgaatacg ccacagtata tgcagacatt cgtttaattc cgcccgctcc gctgaggtta
    29941 agaagccaaa cccgaccatt tcatccagcg atgttgcgcc aaaatgacgg cgggccaccc
    30001 attgcagagt gtgaatatcg cgcaagccgc cagggctact tttgatgtct ggttcaaggt
    30061 tgtagctggt gccatggtaa cgctgatggc gctggttctg ttcttcaact ttcgctgcgt
    30121 agaacttgtc ggaaggccag aatccttcgc taaaaatatg tttttgcagt tcgaggaaga
    30181 gcgcaacatc gccaattaat aagcgggatt cgattaaatt ggtggcgacg gttaaatccg
    30241 ataacccttc caacatgcac tcttcaagcg tgcgcacact atgaccgact tccagcttta
    30301 catcccagag cagcgttaac agctcgccca ctttttgcgc ctgatcgtcc gggagctttt
    30361 tacggcttaa aatcagtaaa tcgatgtcag aaagtggatg cagctcgcca cgaccgtagc
    30421 caccgacggc aaccaatgcc aggtcggcaa tctggctgaa tcccgcttca atccataatc
    30481 gttgcaggag ctggtctatg aactcggtac gtgcctcaat caactgttct gcagagatcc
    30541 cattgtcaaa ggcatcaccc agccaacgct ggaaagtatc gatatgggct tttatcccac
    30601 agacggttaa ttcatcacgg ggccaggcgc atggattttg cggttgaccg ggcagggtgg
    30661 agagagcggt gtttgcgtac tgttctggaa gggtattcat tgtgcgccac ccataagatt
    30721 aaattatcgc attaaaaaag ccggcattcg ccggcttcat cttattcgtc gtgcgagatt
    30781 atcgccggga tggtgtcatc cttgcgtagc gtcaaaattt cgcagccgtt atcagtcacc
    30841 acaatagtat gctcatattg tgcagacaag ctgcgatctt tggtttttac cgtccagcca
    30901 tctttcatgg tgcggatctc ttttttaccc gcgttgacca ttggctcaat ggtgaacgtc
    30961 atccccggtt tcagcacgac gttggtttca cgggagtcat agtgcagcac ctgcggttct
    31021 tcatggaagc cgcgaccaat accgtgtccg caatattcac gaacgacgga gaagccttct
    31081 gcttcgacaa atttctgaat cgccgcaccg atttcgcgca gattaatgcc tggttttacc
    31141 atgcgtagcg ccaggtacag gctttcttgc gtgatgcggc acaggcgttc gcccatgatg
    31201 gtcggcttac cgacgataaa cattttcgag gtatcgccgt ggaaaccatc tttgattacg
    31261 gtgacatcaa tgttaacgat atcgccatct ttcagcagct tagcatcgtc cgggataccg
    31321 tggcacacca cttcattaat agagatgcaa acggatttcg gatagccgtg atagccgagg
    31381 caggcagaaa ccgcgtgttg ttcattaaca atgtaatcat tacagatgcg atccagctcg
    31441 ccggtgctga cgcccggttt aacatacggt tcgatcatct ccagcacttc ggcagccagt
    31501 cggccagcga cgcgcatttt ttcgatatct tctggggtct tgattgagat agccattaat
    31561 tctgtccatc agcgtcggtg ataccgacaa tatatatgta agtgccgtca atggtatcac
    31621 acccgggcaa attgagaatc attctgaatt tcgccaaacg tgccactgaa ggttttctat
    31681 aatagaaaat tcgacgtctg atgctgtaca cagcgccaac aattattggt gtccacgacg
    31741 tatttgtggt ataaagcgcg ccggacttcc gatccatttc gtatacacag actggacgga
    31801 agcgacaatc tcactttgtg taacaacaca cacgtatcgg cacatattcc ggggtgccct
    31861 ttggggtcgg taatatggga tacgtggagg cataacccca acttttatat agaggtttta
    31921 atcatggcaa ctgtttccat gcgcgacatg ctcaaggctg gtgttcactt cggtcaccag
    31981 acccgttact ggaacccgaa aatgaagccg ttcatcttcg gtgcgcgtaa caaagttcac
    32041 atcatcaacc ttgagaaaac tgtaccgatg ttcaacgaag ctctggctga actgaacaag
    32101 attgtttctc gcaaaggtaa aatccttttc gttggtacta aacgcgctgc aagcgaagcg
    32161 gtgaaagacg ctgctctgag ctgcgaccag ttcttcgtga accatcgctg gctgggcggt
    32221 atgctgacta actggaaaac cgttcgtcag tccatcaaac gtctgaaaga cctggaaact
    32281 cagtctcagg acggtacttt cgacaagctg accaagaaag aagcgctgat gcgcactcgt
    32341 gagctggaga aactggaaaa cagcctgggc ggtatcaaag acatgggcgg tctgccggac
    32401 gctctgtttg taatcgatgc tgaccacgaa cacattgcta tcaaagaagc aaacaacttg
    32461 ggtattccgg tatttgctat cgttgatacc aactctgatc cggacggtgt tgacttcgtt
    32521 atcccgggta acgacgacgc aatccgtgct gtgaccctgt acctgggcgc tgttgctgca
    32581 accgtacgtg aaggccgttc tcaggatctg gcttcccagg cggaagaaag cttcgtagaa
    32641 gctgagtaat aaggcttgat aactccccca aaatagttcg agttgcagaa aggcggcaag
    32701 ctcgagaatt cccgggagct tacatcagta agtgaccggg atgagcgagc gaagacaacg
    32761 catctgcggc gcgaaatatg aagggggaga gcccttatag accaggtagt acacgtttgg
    32821 ttagggggcc tgcatatggc cccctttttc acttttatat ctgtgcggtt taatgccggg
    32881 cagatcacat ctccgaggat tttagaatgg ctgaaattac cgcatccctg gtaaaagagc
    32941 tgcgtgagcg tactggcgca ggcatgatgg attgcaaaaa agcactgact gaagctaacg
    33001 gcgacatcga gctggcaatc gaaaacatgc gtaagtccgg tgctattaaa gcagcgaaaa
    33061 aagcaggcaa cgttgctgct gacggcgtga tcaaaaccaa aatcgacggc aactacggca
    33121 tcattctgga agttaactgc cagactgact tcgttgcaaa agacgctggt ttccaggcgt
    33181 tcgcagacaa agttctggac gcagctgttg ctggcaaaat cactgacgtt gaagttctga
    33241 aagcacagtt cgaagaagaa cgtgttgcgc tggtagcgaa aattggtgaa aacatcaaca
    33301 ttcgccgcgt tgctgcgctg gaaggcgacg ttctgggttc ttatcagcac ggtgcgcgta
    33361 tcggcgttct ggttgctgct aaaggcgctg acgaagagct ggttaaacac atcgctatgc
    33421 acgttgctgc aagcaagcca gaattcatca aaccagaaga cgtatccgct gaagtggtag
    33481 aaaaagaata ccaggtacag ctggatatcg cgatgcagtc tggtaagccg aaagaaatcg
    33541 cagagaaaat ggttgaaggc cgcatgaaga aattcaccgg cgaagtttct ctgaccggtc
    33601 agccgttcgt tatggaacca agcaaaactg ttggtcagct gctgaaagag cataacgctg
    33661 aagtgactgg cttcatccgt ttcgaagtgg gtgaaggcat cgagaaagtt gagactgact
    33721 ttgcagcaga agttgctgcg atgtccaagc agtcttaatt atcaaaaagg agccgcctga
    33781 gggcggcttc tttttgtgcc catcttgtaa attcagctaa cccttgtggg gctgcgctga
    33841 aaagcgacgt acaatgtcgc tagtattaat tcatttcaat cgttgacagt ctcaggaaag
    33901 aaacatggct accaatgcaa aacccgtcta taaacgcatt ctgcttaagt tgagtggcga
    33961 agctctgcag ggcactgaag gcttcggtat tgatgcaagc atactggatc gtatggctca
    34021 ggaaatcaaa gaactggttg aactgggtat tcaggttggt gtggtgattg gtgggggtaa
    34081 cctgttccgt ggcgctggtc tggcgaaagc gggtatgaac cgcgttgtgg gcgaccacat
    34141 ggggatgctg gcgaccgtaa tgaacggctt ggcaatgcgt gatgcactgc accgcgccta
    34201 tgtgaacgct cgtctgatgt ccgctattcc attgaatggc gtgtgcgaca gctacagctg
    34261 ggcagaagct atcagcctgt tgcgcaacaa ccgtgtggtg atcctctccg ccggtacagg
    34321 taacccgttc tttaccaccg actcagcagc ttgcctgcgt ggtatcgaaa ttgaagccga
    34381 tgtggtgctg aaagcaacca aagttgacgg cgtgtttacc gctgatccgg cgaaagatcc
    34441 aaccgcaacc atgtacgagc aattgactta cagcgaagtg ctggaaaaag agctgaaagt
    34501 catggacctg gcggccttca cgctggctcg tgaccataaa ttaccgattc gtgttttcaa
    34561 tatgaacaaa ccgggtgcgc tgcgccgtgt ggtaatgggt gaaaaagaag ggactttaat
    34621 cacggaataa ttcccgtgat ggataaataa gggtaagatt ccgcgtaagt atcgcggggg
    34681 cgtaagtctg gttataaggc gttattgttg caggcagttt ggtcacggcc agcgcgcagc
    34741 aaccggagcg tacaaaagta cgtgaggatg gcgagcactg ctcggggcca aaatggcaaa
    34801 taaaatagcc taataatcca gacgattacc cgtaatatgt ttaatcaggg ctatacttag
    34861 cacacttcca ctgtgtgtga ctgtctggtc tgactgagac aagttttcaa ggattcgtaa
    34921 cgtgattagc gatatcagaa aagatgctga agtacgcatg gacaaatgcg tagaagcgtt
    34981 caaaacccaa atcagcaaaa tacgcacggg tcgtgcttct cccagcctgc tggatggcat
    35041 tgtcgtggaa tattacggca cgccgacgcc gctgcgtcag ctggcaagcg taacggtaga
    35101 agattcccgt actctgaaaa tcaacgtgtt tgatcgttca atgtctccgg ccgttgaaaa
    35161 agcgattatg gcctccgatc tcggcctgaa cccgaactct gcgggtagcg acatccgtgt
    35221 tccgctgccg ccgctgacgg aagaacgtcg taaagatctg accaaaatcg ttcgtggtga
    35281 agcagaacaa gcgcgtgttg cagtacgtaa tgtgcgtcgt gacgcgaacg acaaagtgaa
    35341 agcactgttg aaagataaag agatcagcga agacgacgat cgccgttctc aggacgatgt
    35401 acagaaactg actgatgctg caatcaagaa aattgaagcg gcgctggcag acaaagaagc
    35461 agaactgatg cagttctgat ttcttgaacg acaaaaacgc cgctcagtag atccttgcgg
    35521 atcggctggc ggcgttttgc tttttattct gtctcaactc tggatgtttc atgaagcaac
    35581 tcaccattct gggctcgacc ggctcgattg gttgcagcac gctggacgtg gtgcgccata
    35641 atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa aaatgtcact cgcatggtag
    35701 aacagtgcct ggaattctct ccccgctatg ccgtaatgga cgatgaagcg agtgcgaaac
    35761 ttcttaaaac gatgctacag caacagggga gccgcaccga agtcttaagt gggcaacaag
    35821 ccgcttgcga tatggcagcg cttgaggagg ttgatcaggt gatggcagcc attgttggtg
    35881 ctgctgggct gttacctacg cttgctgcga tccgcgcggg taaaaccatt ttgctggcca
    35941 ataaagaatc actggttacc tgcggacgtc tgtttatgga cgccgtaaag cagagcaaag
    36001 cgcaattgtt accggtcgat agcgaacata acgctatttt tcagagttta ccgcaaccta
    36061 tccagcataa tctgggatac gctgaccttg agcaaaatgg cgtggtgtcc attttactta
    36121 ccgggtctgg tggccctttc cgtgagacgc cattgcgcga tttggcaaca atgacgcccg
    36181 atcaagcttg ccgtcatccg aactggtcga tgggacgtaa aatttctgtc gattcggcta
    36241 ccatgatgaa caaaggtctg gaatacattg aagcgcgttg gctgtttaac gccagcgcca
    36301 gccagatgga agtgctgatt cacccgcagt cagtgattca ctcaatggtg cgctatcagg
    36361 acggcagtgt tctggcgcag ctgggggaac cggatatgcg tacgccaatt gcccacacca
    36421 tggcatggcc gaatcgcgtg aactctggcg tgaagccgct cgatttttgc aaactaagtg
    36481 cgttgacatt tgccgcaccg gattatgagc gttatccatg cctgaaactg gcgatggagg
    36541 cgttcgaaca aggccaggca gcgacgacag cattgaatgc cgcaaacgaa atcaccgttg
    36601 ctgcttttct tgcgcaacaa atccgcttta cggatatcgc tgcgttgaat ttatctgtac
    36661 tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga tgtgttatct gttgatgcga
    36721 acgcgagtga agtcgccaga aaagaggtga tgcgtctcgc aagctgagga taatccggct
    36781 acagagagtc gcgctatttg ttagcgtagg gcttcaatga tatagtctgc gccatctgat
    36841 cgtaagtagt tggctttata aggtcaggta tgccgtggtt ttacacggct tttttttgta
    36901 taggcttcag tattcctgag taccgtaaac cctgtcaggg aataaaaaac gcgtgatgtt
    36961 gtctgctact caaccactta gcgaaaaatt gccagcgcat ggctgccgtc atgttgcgat
    37021 cattatggac ggcaatggtc gctgggcaaa aaagcaaggg aagattcgtg cctttgggca
    37081 taaagccggg gcaaaatccg tccgccgggc tgtctctttt gcggccaaca acggtattga
    37141 agcgttaacg ctgtatgcct ttagtagtga aaactggaac cgaccagcgc aggaagtcag
    37201 tgcgttaatg gaactgtttg tgtgggcgct cgatagcgaa gtaaaaagtc tgcaccgaca
    37261 taacgtgcgt ctgcgtatta ttggcgatac cagtcgtttt aactcgcgtt tgcaagaacg
    37321 tattcgtaaa tctgaagcgc taacagccgg aaataccggt ctgacgctga atattgcggc
    37381 gaactacggt ggacgttggg atatagtcca gggagtcagg caactggctg aaaaggtgca
    37441 gcaaggaaac ctgcaaccag atcagataga tgaagagatg ttaaaccagc atgtctgtat
    37501 gcatgaactg gcccctgtag atttagtaat taggactggg ggagagcatc gcattagtaa
    37561 ctttttgctt tggcaaattg cctatgccga actttacttt acagatgttc tctggcccga
    37621 tttcgatgaa caagactttg aaggggcgtt aaatgccttt gctaatcgag agcgtcgttt
    37681 cggcggcacc gagcccggtg atgaaacagc ctgatggggg tcgcttttgc tgaagtatcg
    37741 cctgatatct gcttttgtgt taatacccgt cgtcatcgcg gcgttgtttc tgttgccgcc
    37801 ggtggggttc gccattgtaa cgctggtggt ctgcatgctg gcagcgtggg aatggggaca
    37861 gcttagcggt tttaccactc gttcgcagcg agtatggttg gcggtgttat gcgggttatt
    37921 gttggcgctg atgctttttc tgttgccgga atatcaccga aatattcatc aaccgctggt
    37981 tgaaatctca ctttgggctt cgttgggttg gtggattgtc gcgctattgc tggtgctgtt
    38041 ttacccaggt tccgcagcaa tctggcgtaa ctctaaaaca ttgcgcctta tttttggcgt
    38101 gctaaccatt gttcccttct tctggggcat gctggcgtta cgggcctggc actatgacga
    38161 gaatcattac agtggcgcaa tatggctgct ctatgtcatg atcctggtat ggtgcgctga
    38221 ctccggcgca tatatgtttg gcaaattgtt tggtaaacat aagctggcac cgaaggtttc
    38281 tccgggtaaa acctggcaag gctttatagg tggactcgct actgcagcgg taatctcatg
    38341 gggttatggc atgtgggcga atctcgacgt cgctcccgtc accttactca tttgctctat
    38401 tgtcgcagcg ttagcctcag tgctcggcga tctgaccgag agtatgttta agcgtgaagc
    38461 aggaattaag gacagcggtc atttaattcc aggacacggt ggtattttag atcgtattga
    38521 tagcctgacg gctgcggtac cggtctttgc ttgcttgttg ttactggtat tcaggacgct
    38581 ttaacggaag gtaatatgct gagttttctc tgggatttgg cttcgttcat cgttgcactg
    38641 ggtgtactta tcaccgtgca tgaatttggt catttctggg ttgcccggcg ttgtggtgtt
    38701 cgcgttgagc gtttctcaat agggtttggt aaggcgctct ggcggcgaac tgataagctc
    38761 ggcaccgaat atgttatcgc cctgatcccg ttgggcggtt atgtcaaaat gctggatgag
    38821 cgcgcagaac cggtcgttcc ggaactccgc caccatgcct tcaataataa atctgtcggc
    38881 caacgagtgg cgattattgc cgcaggtccg gttgcaaact tcatttttgc tatctttgcc
    38941 tactggctgg tttttattat tggtgtgcct ggcgtacgtc cggtggttgg cgaaatagca
    39001 gccaattcga tagctgcgga agcacaaatt gcaccaggta cggaactaaa agccgtagat
    39061 ggtatcgaaa cgcctgattg ggatgccgtg cgtttgcagt tggtcgataa aattggcgat
    39121 gaaagcacca ccattacagt agcgccattt ggcagcgacc aacggcggga tgtaaagctc
    39181 gatttacgtc actgggcgtt tgagcctgat aaagaagatc cggtatcttc gctggggatt
    39241 cgtcctcgtg ggccgcaaat tgaacctgta ctggaaaatg tgcagccaaa ctcggcggca
    39301 agcaaggcag gtttgcaagc aggcgacagg atcgttaaag tcgatggtca gcccttaacg
    39361 cagtgggtga cctttgtgat gcttgtccgg gataacccgg gtaaatcctt agcgttagaa
    39421 atcgaaaggc aggggagtcc cttgtctttg acattaatcc cggagagtaa accgggtaat
    39481 ggtaaagcga ttggttttgt cggtattgag ccgaaagtca ttcctttgcc agatgagtat
    39541 aaagttgtac gccagtatgg gccgttcaac gccatcgtcg aagccacgga caaaacgtgg
    39601 cagctgatga agctgacggt cagtatgctg ggaaaattga tcaccggtga tgtgaaactg
    39661 aacaacctca gtgggccgat ttctatcgcc aagggggctg ggatgacagc ggaactcggg
    39721 gtagtttatt acctgccgtt tcttgcgctt attagcgtga acttagggat aattaacctg
    39781 tttccgttgc ccgtacttga cggggggcat ctgctgttcc ttgcgatcga aaagatcaag
    39841 ggcggaccgg tatccgagcg ggttcaagac ttttgttatc gcattggctc gattctgctg
    39901 gtgctgttaa tggggcttgc acttttcaat gatttctctc ggttatgaga gttagttagg
    39961 aagaacgcat aataacgatg gcgatgaaaa agttgctcat agcgtcgctg ctgtttagca
    40021 gcgccaccgt atacggtgct gaagggttcg tagtgaaaga tattcatttc gaaggccttc
    40081 agcgtgtcgc cgttggtgcg gccctcctca gtatgccggt gcgtacaggc gacacggtta
    40141 atgatgaaga tatcagtaat accattcgcg ctctgtttgc taccggcaac tttgaggatg
    40201 ttcgcgtcct tcgtgatggt gatacccttc tggttcaggt aaaagaacgt ccgaccattg
    40261 ccagcattac tttctccggt aacaaatcgg tgaaagatga catgctaaag caaaacctcg
    40321 aggcttctgg tgtgcgtgtg ggcgaatccc tcgatcgcac caccattgcc gatatcgaga
    40381 aaggtctgga agacttctac tacagcgtcg gtaaatatag cgccagcgtt aaagctgtcg
    40441 tgaccccgct gccgcgcaac cgtgttgacc taaaactggt gttccgggaa ggtgtgtcag
    40501 ctgaaatcca gcaaattaac attgttggta accatgcttt caccaccgac gaactgatct
    40561 ctcatttcca actgcgtgac gaagtgccgt ggtggaatgt ggtaggcgat cgtaaatacc
    40621 agaaacagaa actggcgggc gaccttgaaa ccctgcgcag ctactatctg gaccgcggtt
    40681 atgcccgttt caacatcgac tctacccagg tcagtctgac gccagataaa aaaggtattt
    40741 acgtcacggt gaacatcacc gaaggcgagc agtacaagct ttctggcgtt gaagtgagcg
    40801 gcaaccttgc cgggcactcc gctgaaattg agcagctgac taagatcgag ccgggcgagc
    40861 tgtataacgg cactaaagtg accaaaatgg aagatgacat taaaaagctt ctcggtcgct
    40921 atggttatgc ctatccgcgc gtacagtcga tgcctgaaat taacgatgcc gacaaaaccg
    40981 ttaaattacg cgtgaacgtt gatgcgggta accgtttcta cgtgcgtaag atccgttttg
    41041 aaggtaacga tacctcgaaa gatgccgtcc tgcgtcgcga aatgcgtcag atggaaggtg
    41101 catggttggg gagcgatctg gtcgatcagg gtaaggagcg tctgaatcgt ctgggcttct
    41161 ttgaaactgt cgatactgat acccaacgtg ttccgggtag cccggaccag gttgatgtag
    41221 tctacaaggt gaaagaacgt aacactggta gcttcaactt cgggattggt tacggtactg
    41281 aaagtggcgt gagcttccag gctggcgtgc aacaggataa ctggttaggt acaggttatg
    41341 ctgttggtat caacgggacc aaaaacgatt accagaccta tgctgaactg tcggtaacca
    41401 acccgtactt caccgtagat ggcgtaagcc tcggtggtcg tctcttctat aatgacttcc
    41461 aggcagatga cgccgacctg tccgactata ccaacaagag ttatggtaca gacgtgacgt
    41521 tgggcttccc gattaacgaa tataactcgc tgcgtgcagg tctgggttat gtacataact
    41581 ccctgtccaa catgcagcct caggttgcga tgtggcgtta tctgtactct atgggtgaac
    41641 atccgagcac ttctgatcag gataacagct tcaaaacgga cgacttcacg ttcaactatg
    41701 gttggaccta taacaagctt gaccgtggtt acttcccgac agatggttca cgtgtcaacc
    41761 tgaccggtaa agtgaccatt cctggatcgg ataacgaata ctacaaagtg acgttagaca
    41821 cggcgactta tgtgccgatc gatgacgatc acaaatgggt tgttctgggt cgtacccgct
    41881 ggggttatgg tgatggttta ggcggcaaag agatgccgtt ctacgagaac ttctatgccg
    41941 gtggttccag caccgtgcgt ggcttccagt ccaataccat tggtccgaaa gcagtttact
    42001 tcccgcatca ggccagtaat tatgatccgg actatgatta cgaatgtgcg actcaggacg
    42061 gcgcgaaaga cctgtgtaaa tcggatgatg ctgtaggcgg taacgccatg gcggttgcca
    42121 gcctcgagtt catcaccccg acgccgttta ttagcgataa gtatgctaac tcggttcgta
    42181 cttcattctt ctgggatatg ggtaccgttt gggatacaaa ctgggattcc agccaatatt
    42241 ctggatatcc ggactatagt gatccaagca atatccgtat gtctgcgggt atcgcattac
    42301 aatggatgtc cccattgggg ccgttggtgt tctcctacgc ccagccgttc aaaaagtacg
    42361 atggagacaa ggcagaacag ttccagttta acatcggtaa aacctggtaa gtgttctcca
    42421 caaaggaatg tagtggtagt gtagcgatga ctttaggcga tcaatataag atcgccgggc
    42481 cacgcaaaga actgcaccct ccggtgcaaa tgggatggta aggagtttat tgtgaaaaag
    42541 tggttattag ctgcaggtct tggtttagca ctggcaactt ctgctcaggc ggctgacaaa
    42601 attgcaatcg tcaatatggg cagcctgttc cagcaggtag cgcagaaaac cggtgtttct
    42661 aacacgctgg aaaatgagtt caaaggccgt gccagcgaac tgcagcgtat ggaaaccgat
    42721 ctgcaggcta aaatgaaaaa actgcagtcc atgaaagcgg gcagcgatcg caccaaactg
    42781 gaaaaagacg tgatggctca gcgccagact tttgctcaga aagcgcaggc ttttgagcag
    42841 gatcgcgcac gtcgttccaa cgaagaacgc ggcaaactgg ttactcgtat ccagactgct
    42901 gtgaaatccg ttgccaacag ccaggatatc gatctggttg ttgatgcaaa cgctgttgct
    42961 tacaacagca gcgatgtaaa agacatcact gccgacgtac tgaaacaggt taaataagta
    43021 atgccttcaa ttcgactggc tgatttagcg cagcagttgg atgcagaact acacggtgat
    43081 ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg tcacattacg
    43141 ttcatggtta acccaaaata ccgtgagcat ttaggcttgt gccaagcgtc cgcggttgtc
    43201 atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc tggtagtgaa gaatccctac
    43261 ctgacttacg cgcgcatggc gcaaatttta gataccacgc cgcagcccgc gcagaacatt
    43321 gcacccagtg cggtgatcga cgcgacggcg aagctgggta acaacgtatc gattggcgct
    43381 aacgcggtga ttgagtccgg cgttgaactg ggcgataacg tgattatcgg tgccggttgc
    43441 ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa cgtaaccatt
    43501 taccatgaga tccagatcgg tcagaattgc ctgatccagt ccggaacagt ggtaggcgca
    43561 gacggctttg gttatgccaa cgatcgtggt aactgggtga agatcccaca gattggtcgc
    43621 gtaattattg gcgatcgagt ggagatcggt gcctgcacaa ccatcgatcg cggcgcgctg
    43681 gatgacactg ttattggcaa tggcgtgatc attgataacc agtgccagat tgcacataac
    43741 gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg cagcctgaaa
    43801 attggtcgtt actgcatgat cggcggagcc agcgtaatca acgggcatat ggaaatatgc
    43861 gacaaagtga cggttacggg catgggtatg gtgatgcgtc ccatcactga accaggcgtc
    43921 tattcctcag gcattccgct gcaacccaac aaagtctggc gcaaaaccgc tgcactggtg
    43981 atgaacattg atgacatgag caagcgtctg aaatcgcttg agcgcaaggt taatcaacaa
    44041 gactaacgtt ccatcttttg ttcgccaaac tttacggcct gtctcattct tacgattgcg
    44101 gcaggccgtg ttattattgt cgtttcttat attttgacag gaagagtatc ttgactacta
    44161 acactcatac tctgcagatt gaagagattt tagaacttct gccgcaccgt ttcccgttct
    44221 tactggtgga tcgcgtgctg gattttgaag aaggtcgttt tctgcgcgca gtaaaaaatg
    44281 tctctgtcaa tgagccattc ttccagggcc atttccctgg aaaaccgatt ttcccgggtg
    44341 tgctgattct ggaagcaatg gcacaggcaa caggtattct ggcgtttaaa agcgtaggaa
    44401 aactggaacc gggtgagctg tactacttcg ctggtattga cgaagcgcgc ttcaagcgcc
    44461 cggtcgtgcc tggcgatcaa atgatcatgg aagtcacttt cgaaaaaacg cgccgcggcc
    44521 tgacccgttt taaaggggtt gctctggtcg atggtaaagt agtttgcgaa gcaacgatga
    44581 tgtgtgctcg tagccgggag gcctgatacg tgattgataa atccgccttt gtgcatccaa
    44641 ccgccattgt ggaagagggc gcgtcaattg gcgcgaacgc acacattggt cctttttgta
    44701 tcgttggacc ccatgtcgaa attggtgagg gtaccgtact gaaatctcac gttgtcgtga
    44761 atggtcatac taaaattggc cgcgataatg agatttatca gtttgcctcc atcggcgaag
    44821 ttaaccagga tttgaaatat gctggcgaac cgacccgtgt ggaaatcggc gatcgtaacc
    44881 gcattcgcga aagcgtcacc attcatcgtg gcacagtcca gggcggtgga ttgacgaagg
    44941 tgggcagcga caacttactg atgatcaacg cgcacattgc gcacgattgt acggtaggta
    45001 accgctgtat tctcgccaac aacgcaacgc tggcgggtca cgtatcggtt gacgacttcg
    45061 cgatcatcgg cggcatgacc gcagtccatc agttctgcat cattggtgcg catgtgatgg
    45121 ttggcggctg ctccggtgtg gcgcaggacg tccctcctta tgtcattgcg cagggtaacc
    45181 acgcaacgcc gttcggtgtc aatatcgaag ggctgaagcg ccgcggattc agccgtgagg
    45241 cgattaccgc tatccgcaat gcgtataagc taatttatcg tagcggtaaa acgctcgatg
    45301 aagtgaaacc ggaaattgct gaactggcgg aaacatatcc ggaagtgaaa gcctttaccg
    45361 atttctttgc acgctcaacg cgcggtctga ttcgttaatg actgaccagc gtccattaac
    45421 gattgccctg gtcgccggag aaatctccgg cgatatcctg ggggccggtt taatccgcgc
    45481 tctgaaagaa cgtgtgccca acgcccgctt tgttggtgtt gccgggccac gaatgcaggc
    45541 tgaaggctgc gaagcctggt acgaaatgga agaactggcg gtgatgggca ttgttgaagt
    45601 gctcggtcgt ctgcgtcgct tactgcatat tcgtgccgat ctgacaaagc gttttggcga
    45661 actgaagcca gatgtttttg ttggtattga tgcgcccgac ttcaatatta ctcttgaagg
    45721 taacctcaaa aagcagggta tcaaaaccat tcattacgtc agtccatccg tctgggcgtg
    45781 gcgacagaaa cgcgttttca aaataggcag agccaccgat ctggtgctcg catttctgcc
    45841 tttcgaaaaa gcgttttatg acaaatacaa tgtaccgtgc cgctttatcg gtcataccat
    45901 ggctgatgcc atgccgttag atccagataa aaatggtgcc cgtgatgtgc tggggatccc
    45961 ttacgatgcc cactgtctgg cattgttgcc gggcagccgt ggtgcagaag tcgaaatgct
    46021 tagtgccgat ttcttgaaaa ctgcccagct tttgcgccag acatatccgg atctcgaaat
    46081 cgtggtgccg ctggtgaatg ccaaacgccg cgagcagttt gaacgcatca aagctgaagt
    46141 cgcgccagac ctgtcggttc atttgctgga tggaatgggc cgtgaggcga tggtcgccag
    46201 cgatgcggca ctactggcgt cggggacggc agccctggag tgtatgctgg cgaaatgccc
    46261 gatggtggtg ggatatcgca tgaagccttt taccttctgg ttggcgaagc ggctggtgaa
    46321 aactgattat gtctcgctgc caaatctgct ggcgggcaga gagttagtca aagagttatt
    46381 gcaggaagag tgtgagccgc aaaaactggc tgcggcgctg ttaccgctgt tggcgaacgg
    46441 gaaaaccagc cacgcgatgc acgatacctt ccgtgaactg catcagcaga tccgctgcaa
    46501 tgccgatgag caggcggcac aagccgttct ggagttagca caatgatcga atttgtttat
    46561 ccgcacacgc agctggttgc gggtgtggat gaagtcggac gcgggccgtt agttggcgcg
    46621 gtcgtcaccg ctgcggtgat ccttgacccg gcgcgcccga ttgccgggct gaatgattcc
    46681 aaaaagctga gcgaaaaacg ccgtctggcg ctctatgaag agatcaaaga gaaagcgttg
    46741 agctggagtc tgggccgcgc ggaaccccac gaaatcgacg agctgaacat tcttcatgcg
    46801 accatgctgg cgatgcagcg tgccgtcgct gggctgcata ttgcgccgga atatgtgttg
    46861 attgatggta accgctgccc gaaattaccg atgcctgcga tggctgtggt gaaaggggat
    46921 agccgcgtac cagaaatcag tgccgcgtct atcctagcga aagtgacgcg tgacgccgaa
    46981 atggcggcgc tggatattgt tttcccgcaa tatggttttg cccaacataa agggtatcca
    47041 accgcttttc atctggaaaa actggctgaa tatggcgcga ctgaacatca tcgtcgcagc
    47101 tttgggcctg tcaaacgcgc actgggactt gcgtcctgat tcttgtgtcg agattaagta
    47161 aaccggaatc tgaagatgtc tgaaccacgt ttcgtacacc tgcgggtgca cagcgactac
    47221 tcgatgatcg atggcttggc caaaaccgca ccgctggtaa aaaaggcggc ggcgttgggt
    47281 atgccagcac tggcgatcac cgatttcacc aacctttgcg gtctggtgaa gttctacgga
    47341 gcgggacatg gcgcagggat taagcctatc gtcggggcag attttaatgt ccagtgcgac
    47401 ctgctgggtg atgagttaac ccacctgacg gtactggcgg cgaacaatac cggctatcag
    47461 aatctggcgt tgctgatctc aaaagcgtat cagcgcgggt acggtgccgc cgggccgatc
    47521 atcgatcgcg actggcttat cgaattaaac gaagggttga tccttctttc cggcgggcgc
    47581 atgggcgacg tcggacgcag tcttttgcgt ggtaacagcg cgctggtaga tgagtgtgtc
    47641 gcgttttatg aagaacactt cccggatcgc tattttctag agctgatccg caccggcagg
    47701 ccggatgaag aaagctatct gcacgcggcg gtggaactgg cggaagcgcg cggtttgccc
    47761 gtcgtggcga ctaacgatgt gcgctttatc gacagcagcg actttgacgc acacgaaatc
    47821 cgcgtcgcga tccacgacgg ctttaccctc gacgatccta aacgcccgcg taactattcg
    47881 ccgcagcaat atatgcgtag cgaagaggag atgtgtgagc tgtttgccga catcccagaa
    47941 gcccttgcca acaccgttga gatcgccaaa cgctgtaacg taactgtgcg tcttggtgaa
    48001 tacttcctgc cgcagttccc gaccggggac atgagcaccg aagattatct ggtcaagcgt
    48061 gcaaaagaag gcctggaaga gcgtctggca tttttattcc ctgacgagga agaacgtctt
    48121 aaacgccgcc cggaatatga cgaacgtctg gagactgaac ttcaggttat caaccagatg
    48181 ggtttcccgg gctacttcct catcgttatg gaatttatcc agtggtcgaa agataacggc
    48241 gtaccggtag gaccaggccg tggctccggt gcgggttcac tggtggccta cgcgctgaaa
    48301 atcaccgacc tcgatccgct ggaatttgac ctgctgttcg aacgtttcct taacccggaa
    48361 cgtgtctcca tgcctgactt cgacgttgac ttctgtatgg agaaacgcga tcaggttatc
    48421 gaacacgtgg cggacatgta cggtcgtgat gcggtatcgc agattatcac cttcggtacg
    48481 atggcggcga aagcggtgat ccgcgacgta ggccgcgtgc tggggcatcc gtacggcttt
    48541 gtcgaccgta tctcgaaact gatcccgccc gatccgggga tgacgctggc gaaagcgttt
    48601 gaagccgagc cgcagctgcc ggaaatctac gaagcggatg aagaagttaa ggcgctgatc
    48661 gacatggcgc gcaaactgga aggggtcacc cgtaacgccg gtaagcacgc cggtggggtg
    48721 gttatcgcgc cgaccaaaat taccgatttt gcgccgcttt attgcgatga agagggcaaa
    48781 catccggtca cccagtttga taaaagcgac gttgaatacg ccggactggt gaagttcgac
    48841 ttccttggtt tgcgtacgct caccatcatc aactgggcgc tggagatgat caacaagcgg
    48901 cgggcgaaga atggcgagcc accgctggat atcgccgcga tcccgctgga tgacaagaaa
    48961 agcttcgaca tgctgcaacg ctcggaaacc acggcggtat tccagcttga atcgcgcggc
    49021 atgaaggacc tgatcaagcg tctgcaacct gactgcttcg aagatatgat cgcactggtg
    49081 gcactgttcc gccctggtcc gttgcagtca gggatggtgg ataactttat cgaccgtaaa
    49141 catggtcgtg aagagatctc ctatccggac gtgcagtggc agcatgaaag cctgaaatcg
    49201 gtactggagc caacctacgg cattatcttg tatcaggaac aggtcatgca gattgcccag
    49261 gtgctttctg gttataccct cggtggcgcg gatatgctgc gccgtgcgat gggtaagaaa
    49321 aagccggaag agatggctaa gcagcgttct gtatttgctg aaggtgcaga aaagaacgga
    49381 atcaacgccg aactggcgat gaaaatcttc gacctggtgg agaaattcgc gggttacgga
    49441 tttaacaaat cgcactctgc ggcctatgct ttggtgtcat atcaaacgtt atggctgaaa
    49501 gcgcactatc cggcg
//
LOCUS       DADLTI010000009        48666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLTI010000009 DADLTI010000000
VERSION     DADLTI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.009"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN755_000517"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN755_000517"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7379424.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN755_000518"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN755_000518"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7379425.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN755_000519"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN755_000519"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7379426.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN755_000520"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN755_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7379427.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN755_000521"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN755_000521"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7379428.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN755_000522"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN755_000522"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7379429.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JN755_000523"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JN755_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7379430.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN755_000524"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN755_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7379431.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN755_000525"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN755_000525"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7379432.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN755_000526"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN755_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7379433.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JN755_000527"
     CDS             complement(14847..15542)
                     /locus_tag="JN755_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7379434.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JN755_000528"
     CDS             complement(15665..16702)
                     /locus_tag="JN755_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7379435.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="JN755_000529"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="JN755_000529"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7379436.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JN755_000530"
     CDS             complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JN755_000530"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7379437.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDPRSVDDLTAGY"
     gene            19424..19864
                     /locus_tag="JN755_000531"
     CDS             19424..19864
                     /locus_tag="JN755_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7379438.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JN755_000532"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JN755_000532"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7379439.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JN755_000533"
     CDS             complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JN755_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7379440.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JN755_000534"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JN755_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7379441.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JN755_000535"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JN755_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7379442.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JN755_000536"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JN755_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7379443.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JN755_000537"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JN755_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7379444.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JN755_000538"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JN755_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7379445.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /locus_tag="JN755_000539"
     CDS             complement(26602..27879)
                     /locus_tag="JN755_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7379446.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JN755_000540"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JN755_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7379447.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JN755_000541"
     CDS             complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JN755_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7379448.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="JN755_000542"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="JN755_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7379449.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /locus_tag="JN755_000543"
     CDS             complement(30965..32068)
                     /locus_tag="JN755_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7379450.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JN755_000544"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JN755_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7379451.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JN755_000545"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JN755_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7379452.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JN755_000546"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JN755_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7379453.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JN755_000547"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JN755_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7379454.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="JN755_000548"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="JN755_000548"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7379455.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="JN755_000549"
     CDS             37392..37661
                     /locus_tag="JN755_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7379456.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="JN755_000550"
     CDS             complement(37664..38023)
                     /locus_tag="JN755_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7379457.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="JN755_000551"
     CDS             38164..38790
                     /locus_tag="JN755_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7379458.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="JN755_000552"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="JN755_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7379459.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JN755_000553"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JN755_000553"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7379460.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="JN755_000554"
     CDS             41630..42295
                     /locus_tag="JN755_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7379461.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="JN755_000555"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="JN755_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7379462.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="JN755_000556"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="JN755_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="JN755_000557"
     CDS             44278..45327
                     /locus_tag="JN755_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7379463.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="JN755_000558"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="JN755_000558"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7379464.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="JN755_000559"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="JN755_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7379465.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="JN755_000560"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="JN755_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7379466.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
BASE COUNT        11482 a        13475 c        12388 g        11321 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaatt tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacgc
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaattgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc caggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgatt
//
LOCUS       DADLTI010000010        43413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLTI010000010 DADLTI010000000
VERSION     DADLTI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.010"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..413
                     /locus_tag="JN755_000561"
     CDS             <1..413
                     /locus_tag="JN755_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7379467.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JN755_000562"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JN755_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JN755_000563"
     CDS             3028..4098
                     /locus_tag="JN755_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379468.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="JN755_000564"
     CDS             4110..4652
                     /locus_tag="JN755_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379469.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JN755_000565"
     CDS             4660..5175
                     /locus_tag="JN755_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7379470.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JN755_000566"
     CDS             5141..5878
                     /locus_tag="JN755_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7379471.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JN755_000567"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JN755_000567"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7379472.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JN755_000568"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JN755_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7379473.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JN755_000569"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JN755_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7379474.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JN755_000570"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JN755_000570"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7379475.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JN755_000571"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JN755_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JN755_000572"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JN755_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7379476.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JN755_000573"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JN755_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7379477.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JN755_000574"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JN755_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7379478.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQSLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JN755_000575"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JN755_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7379479.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JN755_000576"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JN755_000576"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7379480.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JN755_000577"
     CDS             complement(17760..19520)
                     /locus_tag="JN755_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7379481.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JN755_000578"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JN755_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7379482.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JN755_000579"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JN755_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7379483.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JN755_000580"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JN755_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7379484.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JN755_000581"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JN755_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7379485.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JN755_000582"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JN755_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7379486.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JN755_000583"
     CDS             complement(25127..25573)
                     /locus_tag="JN755_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7379487.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JN755_000584"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JN755_000584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7379488.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JN755_000585"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JN755_000585"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7379489.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JN755_000586"
     CDS             complement(28336..28998)
                     /locus_tag="JN755_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7379490.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JN755_000587"
     CDS             29171..29584
                     /locus_tag="JN755_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7379491.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JN755_000588"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JN755_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7379492.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JN755_000589"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JN755_000589"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7379493.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JN755_000590"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JN755_000590"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7379494.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JN755_000591"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JN755_000591"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7379495.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JN755_000592"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JN755_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7379496.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JN755_000593"
     tRNA            complement(32850..32937)
                     /locus_tag="JN755_000593"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JN755_000594"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JN755_000594"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7379497.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36205
                     /locus_tag="JN755_000595"
                     /pseudo
     CDS             34478..36205
                     /locus_tag="JN755_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004151.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 large subunit"
     gene            36224..36931
                     /gene="hyaC"
                     /locus_tag="JN755_000596"
     CDS             36224..36931
                     /gene="hyaC"
                     /locus_tag="JN755_000596"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7379498.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36928..37515
                     /locus_tag="JN755_000597"
     CDS             36928..37515
                     /locus_tag="JN755_000597"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7379499.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37512..37910
                     /gene="hyaE"
                     /locus_tag="JN755_000598"
     CDS             37512..37910
                     /gene="hyaE"
                     /locus_tag="JN755_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7379500.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37907..38764
                     /locus_tag="JN755_000599"
     CDS             37907..38764
                     /locus_tag="JN755_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7379501.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38898..40442
                     /gene="appC"
                     /locus_tag="JN755_000600"
     CDS             38898..40442
                     /gene="appC"
                     /locus_tag="JN755_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7379502.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40454..41590
                     /gene="appB"
                     /locus_tag="JN755_000601"
     CDS             40454..41590
                     /gene="appB"
                     /locus_tag="JN755_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7379503.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41602..41694
                     /gene="cbdX"
                     /locus_tag="JN755_000602"
     CDS             41602..41694
                     /gene="cbdX"
                     /locus_tag="JN755_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7379504.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41774..43072
                     /locus_tag="JN755_000603"
     CDS             41774..43072
                     /locus_tag="JN755_000603"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7379505.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43187..>43413)
                     /locus_tag="JN755_000604"
     CDS             complement(43187..>43413)
                     /locus_tag="JN755_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379506.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10798 a        10572 c        11558 g        10485 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatcca gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagtct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccacaga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccaaggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcgttccc
    35401 ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 ggaacctgca acctggccga cagagtgccg tggcgtcggc tttaccgaag cgccgcgcgg
    35941 agcgttaggc cactgggccg ccattcgcga tggcaagatt gatctctacc agtgcgtggt
    36001 gccgaccacc tggaacgcca gcccgcgcga tcccaaaggg cagattggcg cttatgaagc
    36061 ggcgctgatg aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc tgcgtactct
    36121 gcacagcttt gatccgtgcc tcgcctgttc aactcatgtg ctgggcgacg acggtagcga
    36181 gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac agaaaagcga
    36241 caacattgtc agccactatg tctttgaagc accagttcgt atctggcact ggctgacggt
    36301 gttatgcatg gcggtgttga tggtcaccgg ttactttatc ggcaagccgc taccttccgt
    36361 cagtggcgag gcgacgtatc tgttctatat gggttacatc aggttaattc acttcagcgc
    36421 cgggatgatt tttaccgtgg tcttgctgat gcggatctac tgggcttttg ttggcaatcg
    36481 ttactcccgc gagctgttta tcgtgccggt atggcgtaaa agctggtggc agggcgtgtg
    36541 gtatgaaatc cgctggtatc tgtttctggc aaaacgtccg agtgccgata taggccataa
    36601 ccccatcgcc caggcggcga tgttcggcta tttcctgatg tcggtcttta tgatcatcac
    36661 cggttttgcg ctgtacagcg aacacagcca gtacgctatt tttgcgccgt tccgttatgt
    36721 ggtggaattt ttctactgga cgggtggcaa ctcaatggac attcacagct ggcatcggct
    36781 ggggatgtgg ctgattgggg cgtttgtgat cggtcatgtc tacatggcgc tgcgtgaaga
    36841 catcatgtcc gacgacacgg tgatctccac catggtcaac ggctaccgta gccacaaatt
    36901 tggcaaaata agtaacaagg agcgttcatg agcgagcaac gcgtggtggt catggggctg
    36961 ggcaacctgc tgtgggccga tgaaggcttc ggcgtgcggg tggcggaacg gctgtatgcc
    37021 cattaccact ggcccgagta tgtggagatt gtcgatggcg gtactcaggg actgaacttg
    37081 ctggggtatg tcgaaagcgc cagccatctg ttgattctcg atgccattga ctacgggctg
    37141 gaacctggaa ggctgcgaac ctatgccgga gaacgcattc cggcctatct cagcgcgaag
    37201 aaaatgagcc tgcatcagaa cagtttctcc gaagtgctgg cgctggcaga tatccgcgga
    37261 catctgccag cacatattgc cctcgtcggg ctgcaacccg cgatgctcga cgactacggc
    37321 ggcagcctga gcgaactggc acgggagcaa ctgcccgctg cggaacaatc ggcgctggcg
    37381 cagttggctg cgtggggaag tgtgccgcaa ccggctaatg aatcgcgttg tctcaattat
    37441 gactgtctgt cgatggaaaa ttacgaaggc gttcgcttgc gccagtaccg gatgacacag
    37501 gaggagcagg gatgagcaac gacacgccat ttgatgcgtt gtggcaacga atgctggcgc
    37561 gcggctggac gccggtcagt gaatcccgtc ttgacgactg gcttacgcaa gcgccagacg
    37621 gcgtggtgtt attaagcagt gacccgaaac gcacgccaga ggtcagcgat aatccggtaa
    37681 tgattggcga attactgcac gagtttcccg actatacatg gcaggtggcg attgctgacc
    37741 ttgagcagag cgaagccatc ggcgatcgct ttggcgcctt tcgctttcct gccactttag
    37801 tgtttaccgg cggaaactat cgcggcgtgc tgaatggtat tcacccgtgg gcggaactga
    37861 taaacctgat gcgcgggctt gtcgaaccgc agcaggagcg tgcctcatga gcgaaacttt
    37921 tttccatctg ctggggccag gaacgcaacc gaacgatgac agtttcagca tgaatccact
    37981 gccgatcacc tgtcaggtga atgatgaacc gagtatggcg gccttggagc aatgtgctca
    38041 cagcccgcag gtgattgcgc tgttaaacga gttacaacat caactaagcg aacgccagcc
    38101 gccgttgggc gaggtgctgg cagtcgatct gttaaatctc aacgccgacg atcgtcactt
    38161 tatcaatacg cttctcgggg aaggggaagt gtcagtgcgc attcagcagg ctgacgacag
    38221 taaaagtgaa atacaggagg cgatcttctg cggattatgg cgggtgcgca gacgtcgcgg
    38281 cgaaaagttg ctggaggaca aactggaggc tggctgcgcg ccgctggcgt tgtggcaggc
    38341 ggcaacgcaa aatctcttgc cgacagattc gctgttaccg ccgcccattg atggcctgat
    38401 gaatggccta ccgttggcgc atgagttact ggcacatgta cgtaaccccg acgcgcagcc
    38461 gcacagcatt aatctgacgc aattacccat cagcgaggct gatcggcttt ttctctcacg
    38521 tctctgtggg ccgggaaata ttcagattcg taccattggc tatggcgaga gctatatcaa
    38581 ctccacgggg ttacgccatg tctggcattt acgctgtacg gacaccttaa aaggcccgtt
    38641 actggaaagt tatgaaatct gcccaatacc ggaagtggtg ctggcagcgc cagaagattt
    38701 ggtcgactct gcgcagcggc ttagcgaggt atgtcagtgg ctggcggaag ctgcaccgac
    38761 gtaaaaagac ggtaagtatc gctttcagtc ttatgaatat cgcaatcggc gaatacctct
    38821 ggtcgtagag tttcaggata aagagggaga tctaacatta tcgggttatt tttctctctt
    38881 cgcctacagg agtgcgcatg tgggatgtca ttgatttatc gcgctggcag tttgctctga
    38941 ccgcgctgta tcacttttta tttgtacccc ttaccctggg gctgattttt ttgctggcta
    39001 tcatggaaac catttacgtg gtcaccggca aaacaatcta ccgcgatatg acgcgcttct
    39061 ggggtaagct cttcggtatc aattttgctc ttggcgtggc taccggcctg accatggagt
    39121 ttcagtttgg tactaactgg tcattctatt ccaactatgt gggcgatatt tttggcgcac
    39181 cactggcgat ggaagcatta atggccttct tcctcgaatc cacctttgtc gggctgttct
    39241 tcttcggctg gcaacggctg aataaatacc agcacctgct ggtgacgtgg ctggtggcgt
    39301 tcggttcaaa tctctctgca ttgtggatat tgaatgccaa cggttggatg caatacccga
    39361 ccggtgcgca ttttgatatc gacaccctgc gtatggagat gaccagcttc agcgagctgg
    39421 tctttaaccc ggtcagccag gtgaaatttg tgcacaccgt catggcgggc tacgtgaccg
    39481 gggccatgtt tattatggcg atcagcgcct ggtatttact gcgcggacgg gagcgcgatg
    39541 tcgcattacg ctcgtttgcc atcggttccg tcttcggtac tctggcgatt atcggtaccc
    39601 tgcaactcgg agacagttct gcgtatgaag tcgcgcaagt acaaccggta aaactggcgg
    39661 cgatggaagg ggagtggcaa acggaacctg ctcctgcacc gttccatgtg gttgcctggc
    39721 cggaacagga tcaagagcgt aacgcctttg ccatcaaaat tcccgcgctg ctagggatcc
    39781 tcgccactca ctcattagat aaacccgtgc cgggtctgaa gaatttgatg gctgaaacct
    39841 acccacgctt gcaacgcgga cgtatggcct ggctgttaat gcaggaaata tctcaaggca
    39901 atcgtgagcc gcatgtgttg caggcattcc gggagctgga aggcgacctt ggttacggca
    39961 tgttgctctc ccgctatgcg ccggatatga atcatgtcac agccgcacag taccaggcgg
    40021 cgatgcgtgg cgcgatacct caggttgcgc cggtattctg gagtttccgc atcatggtgg
    40081 gctgtggttc cctgctgcta ctggtgatgc tgattgcgct tgtccagacg ctgcgtggca
    40141 aaatcgacca gcatcgctgg gtgctgaaaa tggcgctctg gagtttgccg ttgccgtgga
    40201 ttgcgattga agccgggtgg tttatgaccg agtttggtcg tcagccgtgg gcgatacagg
    40261 acatcttacc gacatactcc gcgcactccg ctttaaccac aggacaactg gctttctcac
    40321 tgatcatgat cgtaggtctt tacaccctgt tcttaatcgc cgaagtctac ctgatgcaga
    40381 aatatgcccg tctggggccg agcgcgatgc agagtgaaca accgacgcag caacaggggt
    40441 aaaggagaaa atcatgtttg attatgaaac attgcgcttc atctggtggc tgctgattgg
    40501 cgtgatcctg gtggtcttta tgatctccga cggatttgac atggggatcg gctgtttgct
    40561 gccgctggtg gcgcgtaatg atgatgaacg ccggatagtg attaatagcg ttggtgcgca
    40621 ctgggaaggc aatcaggtct ggttgatcct cgcaggtggg gcattatttg ccgcctggcc
    40681 cagagtgtat gcagcggcgt tttccggctt ttatgtggcg atgatcctgg tgctgtgctc
    40741 gctgttcttc cgtccgctgg cctttgatta tcgcggaaaa attgccgatg cacgctggcg
    40801 taaaatgtgg gatgccggtc tggtcatcgg cagtctggtg ccgccggtag tcttcggtat
    40861 cgccttcggc aacttgttgc tcggcgtgcc gtttgccttc acaccgcaat tacgcgtgga
    40921 gtatctcggc agcttctggc aactgctgac gccattccct ttattgtgtg gtttgctcag
    40981 ccttgggatg gtgattttgc aaggtggcgt ctggttacaa ctgaaaactg ttggtgtgat
    41041 tcatctgcgt tcacagctgg cgactaaacg cgctgcactg ttggtgatgc tgtgcttttt
    41101 gctggcaggt tactggctgt gggtcggtat tgatggcttt gtactgctcg cccaggatgc
    41161 taacggtcct tccaatccgt taatgaaact ggtggcagtg ctacctggtg cctggatgaa
    41221 taatttcgtc gaatcgcccg ttttgtggat cttcccgctg ctggggttct tctgcccatt
    41281 gctgacggtg atggcgattt atcgtggtcg cccgggttgg ggatttttga tggcatcatt
    41341 gatgcaattt ggcgtgattt tcacggcagg catcacgctg ttcccctttg tcatgccgtc
    41401 aagtgtgagt ccaatctcca gcctgacgtt gtgggacagc acttccagtc agctgacgct
    41461 gagcattatg ttggtaatcg tgctgatatt tttgcccatt gtgttgctct acactctctg
    41521 gagctactac aaaatgtggg gacgcatgac aacagaaact ctccgccgta acgaaaacga
    41581 gttgtactaa ggagcaggaa catgtggtat ttactttggt tcgtcggcat tttgttgatg
    41641 tgttcgctct ccacccttgt gttggtatgg ctggacctgc gtctgaaaag ttaacgaacg
    41701 taggcctgat gcggcgcatt agcatcgcat caggcaatca ataatgttag atatgaaaag
    41761 cggaaacata tcgatgaaag cgatcttaat cccattttta tctcttctga ttccgttaac
    41821 cccgcaatct gcattcgctc agagtgagcc ggagctgaag ctggaaagta tggtgattgt
    41881 cagtcgtcat ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc
    41941 agacgcatgg ccaacttggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct
    42001 aatcgcctat ctcggacatt accaacgcca gcgtctggta gccgacggat tgctggcgaa
    42061 aaagggctgc ccgcagtctg ctcaggtcgc gattattgct gatgtcgacg agcgtacccg
    42121 taaaacaggc gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac
    42181 ccaggcagat acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca
    42241 actggataac gcgaacgtga ctgacgcgat cctttgcagg gcaggagggt caattgctga
    42301 ctttaccggg catcggcaaa cggcgtttcg cgaactggaa cgggtgctta atttcccgca
    42361 atcaaacttg tgccttaacc gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt
    42421 accatcggaa ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc
    42481 atcaatgctg acggagatat ttctcctgca acaggcacaa ggaatgccgg agccggggtg
    42541 gggaaggatc accgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt
    42601 ttatttacta caacgcacgc cggaggttgc ccgcagtcgc gccaccccgt tattggattt
    42661 gatcatggca gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc
    42721 cacttcagtg ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact
    42781 ggagctcaac tggacgcttc caggtcagcc ggataacacg ccgccaggtg gtgaactggt
    42841 gtttgaacgc tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt
    42901 ccagacttta cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga
    42961 ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc
    43021 aggttttacg caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa
    43081 aaagagcatt cagttacctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg
    43141 cgtagaccgg aaaaggcgtt cacgccgcat ccggtcactt tcagttttac tctttctcgg
    43201 agtaactata accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca
    43261 caccattgag gattgcgcct ttaatattaa cgccagcctg ttccagacgc tgcattgaca
    43321 agctcacctc tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg gtgccgacag
    43381 agcgtcccac gaccgcagca tcgctcaccg cca
//
LOCUS       DADLTI010000011        41274 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLTI010000011 DADLTI010000000
VERSION     DADLTI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.011"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..153)
                     /locus_tag="JN755_000605"
     CDS             complement(<1..153)
                     /locus_tag="JN755_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease"
                     /protein_id="HAY7379507.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSI"
     gene            complement(508..2679)
                     /gene="glcB"
                     /locus_tag="JN755_000606"
     CDS             complement(508..2679)
                     /gene="glcB"
                     /locus_tag="JN755_000606"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7379508.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(2701..3105)
                     /locus_tag="JN755_000607"
     CDS             complement(2701..3105)
                     /locus_tag="JN755_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7379509.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(3110..4333)
                     /gene="glcF"
                     /locus_tag="JN755_000608"
     CDS             complement(3110..4333)
                     /gene="glcF"
                     /locus_tag="JN755_000608"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7379510.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(4344..5396)
                     /gene="glcE"
                     /locus_tag="JN755_000609"
     CDS             complement(4344..5396)
                     /gene="glcE"
                     /locus_tag="JN755_000609"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7379511.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(5396..6895)
                     /gene="glcD"
                     /locus_tag="JN755_000610"
     CDS             complement(5396..6895)
                     /gene="glcD"
                     /locus_tag="JN755_000610"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7379512.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            7141..7911
                     /gene="glcC"
                     /locus_tag="JN755_000611"
     CDS             7141..7911
                     /gene="glcC"
                     /locus_tag="JN755_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7379513.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(8131..9177)
                     /locus_tag="JN755_000612"
     CDS             complement(8131..9177)
                     /locus_tag="JN755_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7379514.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            9581..11269
                     /locus_tag="JN755_000613"
                     /pseudo
     CDS             9581..11269
                     /locus_tag="JN755_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            11284..12180
                     /locus_tag="JN755_000614"
     CDS             11284..12180
                     /locus_tag="JN755_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7379515.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            12213..12461
                     /locus_tag="JN755_000615"
     CDS             12213..12461
                     /locus_tag="JN755_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7379516.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            12461..13633
                     /locus_tag="JN755_000616"
     CDS             12461..13633
                     /locus_tag="JN755_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7379517.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(13669..14748)
                     /gene="lptG"
                     /locus_tag="JN755_000617"
     CDS             complement(13669..14748)
                     /gene="lptG"
                     /locus_tag="JN755_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7379518.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(14745..15815)
                     /gene="lptF"
                     /locus_tag="JN755_000618"
     CDS             complement(14745..15815)
                     /gene="lptF"
                     /locus_tag="JN755_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7379519.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(15846..16406)
                     /locus_tag="JN755_000619"
     CDS             complement(15846..16406)
                     /locus_tag="JN755_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7379520.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(16418..17242)
                     /locus_tag="JN755_000620"
     CDS             complement(16418..17242)
                     /locus_tag="JN755_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379521.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(17242..18591)
                     /locus_tag="JN755_000621"
     CDS             complement(17242..18591)
                     /locus_tag="JN755_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7379522.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(18637..19395)
                     /locus_tag="JN755_000622"
     CDS             complement(18637..19395)
                     /locus_tag="JN755_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379523.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(19427..20140)
                     /locus_tag="JN755_000623"
     CDS             complement(19427..20140)
                     /locus_tag="JN755_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7379524.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QLADEIMQASLRAIHAALS"
     gene            20314..21006
                     /locus_tag="JN755_000624"
     CDS             20314..21006
                     /locus_tag="JN755_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7379525.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(21055..22554)
                     /gene="pitB"
                     /locus_tag="JN755_000625"
     CDS             complement(21055..22554)
                     /gene="pitB"
                     /locus_tag="JN755_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7379526.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(22846..24705)
                     /gene="gss"
                     /locus_tag="JN755_000626"
     CDS             complement(22846..24705)
                     /gene="gss"
                     /locus_tag="JN755_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7379527.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            24910..25776
                     /gene="yghU"
                     /locus_tag="JN755_000627"
     CDS             24910..25776
                     /gene="yghU"
                     /locus_tag="JN755_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7379528.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(25899..26147)
                     /gene="hybG"
                     /locus_tag="JN755_000628"
     CDS             complement(25899..26147)
                     /gene="hybG"
                     /locus_tag="JN755_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7379529.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(26281..26622)
                     /gene="hypA"
                     /locus_tag="JN755_000629"
     CDS             complement(26281..26622)
                     /gene="hypA"
                     /locus_tag="JN755_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7379530.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(26615..27103)
                     /gene="hybE"
                     /locus_tag="JN755_000630"
     CDS             complement(26615..27103)
                     /gene="hybE"
                     /locus_tag="JN755_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7379531.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(27096..27590)
                     /locus_tag="JN755_000631"
     CDS             complement(27096..27590)
                     /locus_tag="JN755_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7379532.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(27590..29293)
                     /gene="hybC"
                     /locus_tag="JN755_000632"
     CDS             complement(27590..29293)
                     /gene="hybC"
                     /locus_tag="JN755_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7379533.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(29290..30468)
                     /gene="hybB"
                     /locus_tag="JN755_000633"
     CDS             complement(29290..30468)
                     /gene="hybB"
                     /locus_tag="JN755_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7379534.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(30458..31444)
                     /gene="hybA"
                     /locus_tag="JN755_000634"
     CDS             complement(30458..31444)
                     /gene="hybA"
                     /locus_tag="JN755_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7379535.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(31447..32565)
                     /gene="hybO"
                     /locus_tag="JN755_000635"
     CDS             complement(31447..32565)
                     /gene="hybO"
                     /locus_tag="JN755_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7379536.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCVALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(32754..33041)
                     /locus_tag="JN755_000636"
     CDS             complement(32754..33041)
                     /locus_tag="JN755_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7379537.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(33160..34086)
                     /locus_tag="JN755_000637"
     CDS             complement(33160..34086)
                     /locus_tag="JN755_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7379538.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            34204..35244
                     /gene="gpr"
                     /locus_tag="JN755_000638"
     CDS             34204..35244
                     /gene="gpr"
                     /locus_tag="JN755_000638"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7379539.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(35284..35778)
                     /locus_tag="JN755_000639"
     CDS             complement(35284..35778)
                     /locus_tag="JN755_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7379540.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            35969..36854
                     /gene="yghA"
                     /locus_tag="JN755_000640"
                     /pseudo
     CDS             35969..36854
                     /gene="yghA"
                     /locus_tag="JN755_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(37126..37551)
                     /gene="exbD"
                     /locus_tag="JN755_000641"
     CDS             complement(37126..37551)
                     /gene="exbD"
                     /locus_tag="JN755_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7379541.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(37558..38292)
                     /locus_tag="JN755_000642"
     CDS             complement(37558..38292)
                     /locus_tag="JN755_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7379542.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            38544..39731
                     /gene="metC"
                     /locus_tag="JN755_000643"
     CDS             38544..39731
                     /gene="metC"
                     /locus_tag="JN755_000643"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7379543.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            39871..40530
                     /locus_tag="JN755_000644"
     CDS             39871..40530
                     /locus_tag="JN755_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7379544.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(40570..>41274)
                     /locus_tag="JN755_000645"
     CDS             complement(40570..>41274)
                     /locus_tag="JN755_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7379545.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10070 a        11079 c        10254 g         9871 t
ORIGIN      
        1 gatagataat ataagggtta ttgctccagc gacatgtcct ttcagacgta ataccgcgag
       61 tgcaacgaag aagaatatta tcgggatcag ggcgaccaga gcggatagcc ccagtcctcc
      121 catcggcata tacatttggg tccaggtaac catattgttc agtctcttat tatctttata
      181 tgcttgatat acttaaggtt gtaataagca aaagaggact gaactgtaaa atataggcgt
      241 tatactttac agcaacagta cgccgctaac gcaattgcta cctctggcat aacaagtata
      301 tcgggtaagg gtttctgttc cgcacacgca ggcgcagagt atcgttaaga tgtccatatt
      361 gttgttttag gcccgctagt aatgcgctac gggtatttaa tattgttaaa ccctgataat
      421 cgctccggtt atttccggga taaatgtact accgcagtta ctatcatagc cccgacaata
      481 aaacttgccg gggctttttt gacgctatta atgactttct ttttcgcgta aacgccaggc
      541 gtgtaataac ggttcggtat aaccgtttgg ctgtttcacg ccgaggaaga ttaaatcgct
      601 ggcagcttta aaagcacacg agttagcgaa attccccgcc atcggacgat aagccggatc
      661 gccagcattt tgctgatcaa ccactttcgc catattctcc agcgacgcct gcacctgttc
      721 tttggtcaga ataccgtgac gtaaccagtt ggcgatatgc tggctggaga tacgcagcgt
      781 tgcgcggtct tccatcagcg ccacgttgtg aatatccggc acttttgaac aaccaatccc
      841 ctgctccacc caacgcacca cgtaccccag aatcccctgc acgttgttat ccagctcttg
      901 ttggatctct tgcgccgacc agttagcgtt ttcagccacc ggaatagtca gcagatcgtc
      961 cagcagcggt tcaaattcag cattgaactc ggtctgggca atgttggctt gcacgctctg
     1021 tacgttggtt tggtggtagt gcagcgcatg gagcgtagca gcggttggtg acggaaccca
     1081 ggctgtgttt gccccggcac gcagttggtc gcccttctgg ctgtacatgt ctgccatcag
     1141 gtccggcatt gcccacatgc ctttaccgat ttgcgcttta ccgcgcagcc cacagaacag
     1201 accggaaagc acgttattac gttcgtaggc tttgatccac ggcgtcgatt tcatctggtt
     1261 tttacgcagc atcgggccag cttccatcac cgaatgcatt tcatcgccgg tacggtcgag
     1321 gaaaccggta ttgatgaacg ccacgcggtt gcgcgcctga gcgatacagc tccgcaagtt
     1381 cagcgaggtc cggcgttctt catccataat gcccattttc agggtattcg gtactatacc
     1441 gagcattgtc tcaatgcggg taaacagttt gttggcgaac gccacttcct gcggaccgtg
     1501 cattttcggt ttcacaatat agacgctgcc agtgcgcgag tttttctgca cttttaaatc
     1561 atagagggca atcgcgccag tcatgacgcc atcaagaata ccttccggga tttcattgcc
     1621 ttcgctgtcc caaatcacag gaatggtcat caaatgaccc acgttgcgga taaacagcaa
     1681 cgagcgtccg tgcagagaaa tttcagagcc atcggcggcg gtgtactgac gatcgtcatt
     1741 cagtttacgt acgatttgcc gaccgttttt ctccattttc tcttgcagag tcccctgcat
     1801 caggcccagc agattgcggt acagcaggat tttatcttcc gcatcaaccg ccgcgaccga
     1861 atcttcgcag tcgagaatgg tactgatagc agcttcgacg ataacatcgt tgatgtgcgc
     1921 cggatcgtct ttgccaatcc gcccgttggc atcaatttgc agttcaatat gcaggccgtt
     1981 atttttcagc aaaatgcagg tcggcgcagc ggcatcgcca cggtaaccga caaactgtgc
     2041 tggagtacgt aacgtggttt ctttaccatt tttcaactgg atgcgtaatt gtttatcgac
     2101 caccttaaac gccaccacat cctgatagct gccgttttcc agcggtagtg attcatcgag
     2161 gaaacgtcga acccaggcga taacctgctc accgcgctgc ggatcgtagc cactgaccat
     2221 cgccccttcc tgcgggatga tgtcgctgcc gtataacgca tcgtacagtg agccccagcg
     2281 agcgttcgcc gcgttcagcg cgtagcgggc gttcattgcc ggaaccacca gctgtggccc
     2341 cgcctgactg gtgatttcgc tgtcaatgcc cgtggtttcc accgtcacgc gctccggttg
     2401 cggcaccagg tagcccagtt cacgaaggaa agatttatag gccgctttat cttttaccgg
     2461 ccccggattg ctgcgatgcc actcatcaag cgccgcctga atgcgatcgc gttctgccag
     2521 caactgacga ttttctggtg ccagatcgtg aacgatctca tcaacattgt gccagaatgc
     2581 agcagcgtcc agttccacgc ccggcaaaac ttctccatcc acaaaacgtt taaaattggc
     2641 gtcaatgcgt aaacggccct gggttatggt ttgactcatt gtttctctcc tcgttttcgc
     2701 ttatttcgcc aacaccgctg ctgccgcttt cgcgacctgc gcatcctgtg ctccggttaa
     2761 accagaaacg cccacggcac caataatttg cccatcaaca acaaccggta cgccgccttc
     2821 cagcgacgtc agtaacggcg cagtcacgaa cgcggtacgt ccgttgttca ccatctcttc
     2881 atagccctta gtttcacgac gccccagcgc ggcggtacgc gctttctcct gggagatgta
     2941 agccgcaatc ggcgcgcaat cgtccatgcg acttaacgcc agcagatgac cgccgtcatc
     3001 ggcaacagca atggaaacag accagttatt tttctgcgcc tcttcctgac ctgcggcaat
     3061 aattgcactc gccatttgct ggctaagaat gactttagtt ttcatttttt tattcctttt
     3121 caagggcttg ttctacaatt tcaatccagt gacgcacaga ggtacgaccg gcgctcgcca
     3181 gatgcgtctg gcaaccaatg ttggcggtga cgatcatttc cggtttgccg ctttccagcg
     3241 cattcatttt gttatcccgc agctggcgtg ccagatcggg atgcgttaac gcatatgttc
     3301 ccgctgaacc gcagcacaga tggctgtcgg gaacgtccgt taaggtaaat ccaagacgaa
     3361 gtaacacgtt ttccacttcg ccgttcagct tttgcgcatg ttgtagggta cacgggcagt
     3421 ggaaggccag ttttttatcg ccgcgaattg ccagtttttc cagcggttcc tcgcgcagaa
     3481 gttcgactaa atcgaccgcc agttcactga cctgacgcgc tttatcggca tataacgcat
     3541 cgtttttcag catctgccca tactctttga cgaacgcgcc gcaaccgctg gcggtttgca
     3601 aaattgcctc ggcacctgct tcaatcgcgg gccaccaggc atcaatatta ttgcgcgccc
     3661 gtgccaaccc tttctcctgc gcattaagat gatagtccac cgcgccacaa cagcctgctt
     3721 cgttagctga catgacgctg atccccagac gatccagcac tcgcgcagtt gccgcgttgg
     3781 tgttgggcga aagcgtaggc tgggcgcagc cttccagcat taaaacccga cgcttatggc
     3841 gcagcggcgg acgcggttta gctttcaccg tttcagcagg cagttttgct ctgacctgtt
     3901 ccggtaaaaa cggtcgcagc accagcccta cctgcgtcag cgcacggaag accgccggac
     3961 gcggcactac ctggcgcaat ccttcgcgca gcattcgctc cggcagtggg cgtttcactt
     4021 tctgctcgac aatatcacgc ccgatatcca gcaaattgtg atagcgcaca ccagaaggac
     4081 aggtggtttc acaattgcgg caagtgaggc agcgatcgag atgctcctgt gttttaagcg
     4141 tgacttcgtt gccttccagc acctgtttaa tcagatagat gcgcccgcgc ggcccgtcca
     4201 gttcatcgcc cagaagctga taggttgggc aggttgcggt acaaaatccg cagtgaacac
     4261 aggcgcgcag gatgctgtcg gcttccagcg cgcgcgcgtt ctgccgcatc tcttcggtta
     4321 atcgggtttg catagcctgc tcctcaaagt tccgcgtaca tgcgaccggg gttaaacacg
     4381 ccgcaagggt cgagctgctg tttaagctgc tggtgatagc agaataaagg agccgatagc
     4441 ggggcaaagc caccatctcc ggcactaaag cgggtcgcat gaccgccagc gttgcgggcg
     4501 atgcgatgga tttgattgtc atcggctgtc gatttcagcc agcgtaacgc cccgccccag
     4561 tcgatcagtt gctcgccggg taaatccatc atcggcgcat cgctgggtaa tgaaatgcgc
     4621 cataaggtac ctggtaacga gaagaacggc agttgttgtt cacgcaattg ctgccagaac
     4681 tgacccgcaa cctcttcgcc acccagcagt tcacgcgctg cttttaccga tccttcgccg
     4741 ccctcaaggc ggatccacaa cgcattgtcg aagtaacata agccactaat gggtaatggc
     4801 tggagttgcc actcggcgat ttcactcatg gcttcttgca ggctgatttc ccgacgcagg
     4861 ctcagagagg cacgcggtcg cggtaacact ttcattgaga tttcagtgag cacgccaaga
     4921 caaccgtagc ttccggccat taaccgtgag agatcgtatc cggcaacgtt tttcatcact
     4981 tcgccaccaa aacgcagatg ttttccagtg ccggtaatga tgcgcgtgcc gaggacaaaa
     5041 tcgcggaccg aaccgctcca cgggcgacgc ggccccgcca gcccgcaggc gaccatcccg
     5101 ccccaggtgg cttcttcacc ataatgcggc ggctcacagg gaagcatttg cccggcgctc
     5161 tccagcgccg cttcaatcgc caccagcggc gttccggcac gagcggttat caccagctcg
     5221 gtcgggtcgt aattaacaat gccgcgatga caacgcacat ccagcgtttg cccggtgact
     5281 gggcgaccta aaaaggcttt gctattgctg ccctgaatca ccagcggcgt tttatcgcta
     5341 attgcctgat tcacctgctc cagcagcgcc tgactgtaat cacactcgcg tagcatcaaa
     5401 agcgctccag ttcaggaaaa ggtaaatgac cgtgatgcac atgcatggca ccaaattcag
     5461 cacagcggtg tagcgtggga atgtttttcc cagggttcag caaaccatcg gggtcaaacg
     5521 ccgccttgac cgcatggaag gtcgtgattt catcgctgtt gaactgggcg cacatttgat
     5581 tgattttttc tcgcccgatg ccatgttccc cactgatgct gccgccaact tcaacgcaga
     5641 gttcgaggat cttcccgccc agctcttccg cgcgggcaaa ttcaccaagt tcgttggcat
     5701 cgaaaaggat taacgggtgc atgttgccat ctccggcatg aaagacgttg gcaacacgta
     5761 aatcatattg ctgcgataaa cgggcaatgc cttccagtac gccaggcagg gcgcgacgcg
     5821 ggatggtgcc atccatgcag tagtaatccg gggagatacg tcctaccgcc gggaacgcat
     5881 ttttgcgacc ggcccagaaa cgtacgcgct ctgcttcgtc ctgtgccaaa cggacgtcag
     5941 tcgcgcccgc tttcaacaag atgtagttaa cccgctcgca gtcttcctgt acgtcagact
     6001 ccacgccgtc cagttcgcat aacaaaatcg cttcggcgtc gacgggataa ccggcatgaa
     6061 taaaatcttc cgccgcgcgg atcgacaggt tatccatcat ctccagcccg ccggggataa
     6121 tgccattggc gatgatgtca ccaaccgcaa gtccggcttt ttctaccgag tcaaagctgg
     6181 ctaacagaac ccgcgccacg ggcggcttcg gcagcagttt taccgtcact tcggtggtca
     6241 cgccgagcat accttccgat ccggtgaaca gcgccagcag gtcaaaacca ggtgaatcca
     6301 gcgcgtccga tcccagcgtc agcgcctcgc cgtccagcgt ttgcacttca attttcagca
     6361 ggttatgtac ggtcagacca tatttcaggc agtggacgcc accggcattt tccgccacat
     6421 tgccgccaat tgaacaggcg atttgtgagg aaggatccgg cgcgtaatag agattatgcg
     6481 gcgcaacggc ctgggagatc gccaggttac atacgcccgg ctgcacgcgc gcgcggcgac
     6541 caacggggtt aatgtcgagg ctctctttaa agcgcgccat caccaacaac acaccttttt
     6601 ccagcggcag cgcgccacca gaaagcccgg tgcctgcacc acgggtcacc accggtacac
     6661 gcaggcgatg gcagacagcc agaatcgctg tcacctgttc catttgctta ggcagaacaa
     6721 ccagtaatgg acgcgtgcga tacgcgctca acccgtcaca ctcgtaagga atgatctcct
     6781 catcggtatg caggatctca agtccaggga catgctcacg cagtgccatc agtaccgatg
     6841 tgcggtcgac atcgggtaaa gcgccatcaa gacgctcttc gtacaagatg ctcatgagta
     6901 ggcttcgctt tgttgtgtgg cagctgattt ttgcgcgctg cttctgtgaa cagttattaa
     6961 gcgggctttt cgttttcgtc tatctcttta gctaccggtc agaccatttt ttttccagct
     7021 ctgtgacctt gtcttggtta acttaatgtt aaattgatgt aacataatca cttgcgtgat
     7081 gtgcgtgttt tgcgagttaa gaacagaaaa attggtccta cctgtgcacg aggtccggga
     7141 atgaaagatg aacgtcgccc tatttgcgaa gtggttgcag agagtatcga acggttaatt
     7201 atcgacggcg tactgaaggt cggtcagccg cttccctcgg aacgtcgact gtgtgaaaag
     7261 ctcggcttct cacgctccgc actgcgtgaa gggctgaccg tgctgcgcgg gcgcgggatt
     7321 attgaaacgg cgcagggtcg cgattctcat gtcgcacggc ttaatcgggt gcaggacacc
     7381 agcccgctga tccatctgtt cagtacgcag ccgcgaacgc tgtacgatct gctcgacgtt
     7441 cgcgcattac tggagggcga atcggcaagg ctggcggcaa cgctgggaac gcaggctgat
     7501 tttgttgtga taacccgctg ttatgaaaaa atgctcgccg ccagtgagaa ccacaaagag
     7561 atttcgctga tcgaacatgc gcagttggat cacgctttcc atctcgccat ttgtcaggct
     7621 tctcacaatc aggtgctggt gtttacgctg caatcattga ccgatctgat gtttaattca
     7681 gtgtttgcca gcgtaaataa tctctaccat cgaccacagc aaaaaaagca gatcgatcgc
     7741 cagcatgcgc ggatctacaa cgcggtgttg cagcggctgc cgcacgtcgc tcagcgcgca
     7801 gcgcgcgatc atgtacggac cgtgaaaaag aatctccacg atatcgagct ggaaggccac
     7861 catttgattc gctcggcggt gccgctggag atgaacaagg tgggaatgtg agcagagttt
     7921 taccctatcc ccaaggcgag gggaccgccc ctgcacttaa caggatctgg tgttaaatct
     7981 gttcatctat accctctccc gaatgaccgc ccctgcgcta aacaggatct ggtattaaat
     8041 ttgcttttca tacaatcggt aacgcttgta cggctccgcc ccaatgcgtt ccagcatgtt
     8101 attcatgcct gtattggttt cgaggatcca ttacatctcc agcgcatcga tcttccggcg
     8161 ggcaaacgga tcgcgtaagg cttcaatcaa taacagcgca atcaccgggc cgatgcggct
     8221 gaactgatac tcgtcgcgca cgcccatcag cggtactcgc gcagttcgca caccgctgac
     8281 tttcaaacgc cacagcaatt ttgcccagcc gaagggaaag agcgatccgt tcagatcggc
     8341 aatcgcctcg ttgatgttag gcaagccgac aataaacgcg cagggtgcag aatcaatctc
     8401 agcgatgtag atcatatcgt ccggcaccag atatttaagt tggtcgccca tggtcgcgaa
     8461 ttcatgttcg gtaaacggca caaatcccca gttgtgctgc cagccagagt tgaaaatctc
     8521 acgcaggatc tgcatctctt cggcaaaccg ctgacgattg atgcagcgaa tggtcacctt
     8581 tttgcgcacc tgatccatca gtttttttag cgccggagag aaagtgagat cggttcgctg
     8641 catccaccac gccagtaaat caatgccttt gtgataaccc agttgttcaa tatgcgcggc
     8701 ataccacggt ttgccgtgtg gcatcatcgc acagggtggt gtgtcaaaac cttcaatcag
     8761 taatccgctt tcctgattga tattcaggct gaaaggcccg ctgatcttac ttgcaccttg
     8821 tgacttcaac cacgcttccg ctgcgccaaa caacgcggca aaatcctgcg gatcatcaat
     8881 ggcgtcaatc atgccgaaat gaccggtatc ttcaccgtaa cgctcacggt gcaaggtatc
     8941 tatttgcgcg gtaatacgcc caactatctg ccccgctttt tttgccaccc acgcctgcca
     9001 gatgatatgg tccgtccccg gatttttcgc agacaaatgc tcgttgcgtt caatgaataa
     9061 agggggtatc cagtttggat cgtcgggata aagtgatgac ggaaaagcga taaatgcctt
     9121 aaggtcattt ttattaatta ctttttcaat tttaatcatc agcagatcgc attccatatc
     9181 tctgtggata acaaataatt aatggtgaaa aacaggttgc aacagcaacg agcggcatct
     9241 taataatcaa ctcaatgaat acaaacgacg aataaatagt attaattaat gattatgata
     9301 aggtcagagg ctacacagaa acgcctcaat gccttttgtc gatttaaggc gaccggacaa
     9361 ggcttcttct taatatattc ataaattaaa tatcattcat aataaaaata aaaaactcaa
     9421 actcattatt taaatcaatg ttcattttca caccgatgaa aatactatct catttatctt
     9481 ttatgcctat ataatccacc gcattattgt cattttattt ttgatatcca attttttcgt
     9541 ggtgtatatg tctaataaaa tctttacgca ttccctacct atgcgctatg ccgattttcc
     9601 aacgctggtt gatgctttgg actacgccgc tctgagtaga gccggaatga atttttatga
     9661 cagacgttgc caacttgaag atcaactgga atatcagaca ttaaaaacgc gtgccgaagc
     9721 tggtgcgaag cggttgttat cgctgaacct gaaaaaaggc gaacgcgtgg cgctgattgc
     9781 cgaaacgagt agcgggtttg tagaggcttt ttttgcctgt cagtatgctg gcttagtcgc
     9841 cttcccgttg gcgattccaa tgggcgtcgg tcagcgggat tcctagagcg ccaaactcta
     9901 gggtttactg gcaagttgcc agcccgcagc cattatcact ggtgatgagt agttgccact
     9961 ggtcaatgcc gcgacgcata acaaccccga attacatgtt ttaagccacg cttggtttaa
    10021 ggcattaccg gaagccgatg ttgcgctcca gcgtccagtt ccgaacgata tcgcctacct
    10081 ccagtacacc tccggcagca cccgttttcc ccgtggcgtc attatcaccc atcgcgaagt
    10141 gatggctaat ctacgtgcta taagccacga tggcattaaa ttacgcccta gcgaccgctg
    10201 cgtctcctgg cagcctttct accatgatat gggactggtc ggctttctcc tgccccccgt
    10261 cgccacgcaa ctttcagtag attatttgcg cactcaggat tttgccatgc gtcctttgca
    10321 atggcttaaa ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc cgtttggcta
    10381 tgaattgtgc caacgccgcg tgaatgaaaa agatctcgct gaactggatc tttcctactg
    10441 gcgcgtcgct ggtattggtg ctgagccgat ctccgcagaa caactccatc aattcgctga
    10501 atgtttccgt caggttaact ttgacaataa aactttcatg ccgtgctacg gactggcaga
    10561 aaatgcgctg gctgtcagct tctctgatga agcctccggg gttgtggtta acgaagtgga
    10621 tcgcgacatc ctcgaatatc agggtaaagc cgtcgcgccg ggtgcagaga cacgcgccgt
    10681 atcgactttc gtcaactgcg gcaaagcgtt gccggaacat ggtattgaaa tccgcaatga
    10741 agcaggtatg ccggtcgcgg aacgtgtggt aggccatatt tgcatctccg gtcccagtct
    10801 gatgagcggt tactttggcg accaggtttc gcaagacgag attgccgcga cgggctggtt
    10861 agacaccggc gacctcggtt atctgctgga cggttatctg tatgtcaccg gacgcattaa
    10921 agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat atatagcgga
    10981 acaggaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgccc aggaaaaaat
    11041 cattttgcag atccagtgtc ggatcagcga cgaagaacgt cgcgggcagc ttatccacgc
    11101 gctggcggca cggatccaaa gcgaatttgg cgtgaccgcg gctatcgatc tgttgccgcc
    11161 ccacagtatt ccccgaacgt cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta
    11221 tcagaaggct tatgctgcca gtcttaatgt gcaggaatcc ctggcatgaa tcaaactgtc
    11281 gcggtgacgg gcgctaccgg gtttatcggt aaatatatta tcgataacct gctcgcccgc
    11341 ggctttcacg ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga taatctcacc
    11401 tgggtgcgcg gttcgctgga agatactcat tcacttagcg agctggttgc cggagccagc
    11461 gtagtggtcc attgcgccgg gcaagtgcgc gggcacaaag aagagatttt cacccgctgt
    11521 aacgttgacg gcagcctgcg cctgatgcaa gcagcaaaag agagcggctt ttgccaacgt
    11581 tttctgttta tctcttcgct ggcggcgcgc catcccgagc tctcctggta tgcaaaatcc
    11641 aaatacgtcg ccgagcaacg actggcagca atggcggacg aaattacgct gggcgttttt
    11701 cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg
    11761 ctacgcggcc tgctgccacg acttggtgcg ccagatacac aactctcttt tcttcacgtc
    11821 accgattttg cgcaagcagt gggtcagtgg ttaagcgccg aaactgtaca gacgcaaacc
    11881 tatgaattat gcgatggcgt cgccggtggc tatgactggc aacgcgtacg acagcttgcc
    11941 gccaaagccc gttgtggttc cgtgcgaatg gttgacattc ctctgccggt actcacaggc
    12001 cttgcggata tcagtaccgc gttgagtcgc ctggcaggta aagaacctat gctgacccgc
    12061 tcgaaaattc gtgaattaac ccacgccgac tggtcggcaa gtaataaccg tatttctgaa
    12121 gatatcaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg gctattttga
    12181 ttgtcttgtt ttaaaggtgt gttaacgttt ctatggtaaa tcgtgaaata gtaatggatt
    12241 acatcctttc ctgtttacag gatttggtcg aaaacggtgt ggaaattaag cccgacagcg
    12301 atctcgtcaa cgatcttggc ctggagtcga tcaaagtgat ggatctctta atgatgctgg
    12361 aagacagatt cgatatttcc attcctatta atattctgct gaatgtcaaa ccccctgcgc
    12421 aattaatgga aactctactt ccatggctgg agaataaata atggggctat acgataaata
    12481 tgcgcgcctt gctggcgagc gtctgcaatt tagtgacaac gggttaacac cgtttggcac
    12541 ctgtattgat gaagtgtatt ccgccaccga aggccggatc ggcaataaaa aagtgattct
    12601 ggccgggacc aataattatc tcggtttaac ctttaatcat gatgccatca gcgaaggtca
    12661 ggccgcgtta gcggcccaag gcaccggcac caccggttct cgtatggcaa acggcagtta
    12721 tgcgccgcac ctggcgttag agaaagagat tgccgagttt ttcaatcgcc ctaccgctat
    12781 cgttttttca accggttata ccgctaattt aggcgtcatc agcgcacttg ccgaccataa
    12841 cgccgtggtg ttactggatg ccgacagcca cgccagtatt tatgacgcct gttctctcgg
    12901 tggcgcggaa attattcgtt tccgccataa cgatgctaaa gatctggaac ggcgcatggt
    12961 tcgcctcggt gaacgggcga aagaggcgat tatcatcgtt gagggcatct acagcatgct
    13021 cggtgacgtt gcgccgctgg cggaaattgt tgatatcaaa cgtcgtctcg gtggttattt
    13081 gattgtcgat gaagcgcatt cgtttgtcgt gttaggcgca acggggcgcg gactggctga
    13141 agctgtcggc gttgaagatg atgtggatat tatcgttggt actttcagta aaagtctggc
    13201 ctcgattggc ggttttgctg tcggttcgga agcgatggaa gtgctgcgct acggcagccg
    13261 cccgtatatt tttaccgcct ctccttctcc gtcctgcatt gcgaccgtgc gctcgtcgtt
    13321 aagaactatt gccacacaac cagagctgcg gcaaaagctg atggataacg ccaaccatct
    13381 gtatgacggt ctgcaaaaac tgggttatga gttaagttca catattagcc cggtagtgcc
    13441 agtaataatc ggttcgaaag aggaaggttt acgcatctgg cgcgagctta tctcgccggg
    13501 cgtgtatgtg aatcttatcc tgccgcctgc cgcgcctgca ggcatcacgc tgctgcgctg
    13561 tagcgtaaat gctgcccaca gtcgtgagca aatcgatgcc atcattcagg catttgctac
    13621 tctgaagcag taatgtgagc ccgccgtggt gtttgcgcca cggcgggttt aatgttgtct
    13681 gtcataccaa tacaccaggc tcaacgccac caacacaaac gctaccggta aaccgagcgc
    13741 ggtaacaggt gcgcttaacg caaatagcaa tcccagatta accatgattt gatagctgat
    13801 ccaggtgagt aagcccacga tgacgcctac agcgagacgg ctacccatcc caggcgaacg
    13861 cggggcacta aaggtaaacg gcacagccag caaaatcatc gccagggtca ggatgggttg
    13921 ccccagtttt tcccacagag caaggcggta ttcgctgctg ggttgcccgg tttcctgcaa
    13981 ataatggatg taatgattaa gctgacggac agaaaaagta ttccccggca ttgataactc
    14041 ttcaagattc atactggtga aggccgattg ccaggcaaga ttatctaatg tttccagcgt
    14101 ttctttgcca ttcagccatt ttttatgatt cacaccatgc aggacccacg ttttgtcgtc
    14161 ttcaatggtg gcactgcgtg cataaatgta ggattcaagc gagagatcgt cgcgataatg
    14221 aaatatctcc acgcccaccg gctggccctg ctcgttcagc gatttcactg tcacaaattc
    14281 attaccacgc ctggcccaca gcatattgcc ggtaatgtca tcgtcctccc ccaacgcggt
    14341 ggcggtcgat ttgatttgca acgcctgttg ctgcaatggc gacgccaccc actcatctat
    14401 cgcgcctaac gaaacagtcc acaatatccc ggcaaccagc gccaccagtg caatacggaa
    14461 gatagaaaac cccgtgctgc gaatggcggt aagctcactg tttttcgata actgccccag
    14521 tccaacaata ccacccaata acgcgataaa cggcgaaagt tcgaccagtg tgcgcggtaa
    14581 ggtcattagc accaccagca ccgcctgagt ccagcgataa ccgcccgggc tgacaccatc
    14641 cagttcgtta atcaggttga aggtggtaaa aagcggcagc aatagccctg cagcggcggc
    14701 aaaaccgaga aagagatgac ggattaaata gcgactgaaa acattcatcg ggaaaatttc
    14761 tgcagcaaag agaagtcacg tgcgaccagt atcagcaggc ccattagcat gagtccgggc
    14821 actaaccaca aaccaggaat gaggggaatc gcgccgttag ccaccagtgt acggcagacg
    14881 tcgccgccat aaaaaatggc aataaacagc aacgtcagtg gcaataacgt tgaaaatcgc
    14941 ccttgccacg gttttacccg acttaatgaa atggccagca gcgccatcaa caatgcggtc
    15001 aggccacggc tttgtcgcca ttgcagttcg gcatgatcgg caggaaaggc ggagcgcgcc
    15061 agctccgtga ccgatttcgc tttgcgtttg acgttcgggc tttgatccag cggattcaga
    15121 tgtaattgca gattacggta gatctgttcg ttgtcgtcac gcccctgatg atcgagaaga
    15181 taggcagtgc cgttgtgcaa cataacggtc ggtttctcgg gtgatgggtc aaccacatca
    15241 accgaacggg cgcggaaaat acgggttcga ttggcagtag aagtataaat cagcgcgtca
    15301 gtcaggcgat tattatcctg atcaaccgtc tgcgaaagga tcattcgtcc gttatcgtta
    15361 gtgttaaatt tctttgcccg caactggcga acatccagct ccgactgcga ctgctgctcc
    15421 agttgataaa tctgcgcata agcccacgga cgaccataca tcgacagaag ggtgacaaaa
    15481 atacttagcg gtatcgccag ataaagaacg gctttgtaca agcgtcccgg actgcccccc
    15541 gccgcagaga tagcggtaat ttccgagtcg gtatacatct gccctagcgt cacgccaact
    15601 gacacataca gaccaacagg taacaacatc tccagtgcaa tcagtacttt gtagaaaacg
    15661 atatccagca caacatcaag agctaatgtc ccatttgccg cttcggtcag ataacgctgt
    15721 gcggagtagc tggcgaaaat aaagatcaga aaaccgacga taatcagcac cagacggcgc
    15781 gtaccacgca tgatgtagtg ttcaaccagc ttcatctcac taacgccctc aatcctttga
    15841 gttttttatt cattgctcag tttctgcgcc aacgcaatga catgatctac ctcttccggc
    15901 gtcaggctgc cgtcctttgc ccactgaatt tgcccggctt tattcagcac cagaatggtt
    15961 gaactctgct ccggcaatcg ccaggcaacg cgtccctgtc cattgccgtc aataacgaat
    16021 tgcgcccacg gatagcggcg tttatttttt tcgattttgc caacaacaaa atagccagta
    16081 ccaaagatgg catcatcggt gttcacaatg gtggtgggct gaaaacgatc ctgcggaaag
    16141 tttgccgctt caacagcttt aattaacagt gagttctttt tcttcgccga cttacgcccg
    16201 gcgatatact gaataatgcg gacttttccc gctaattgtg tgctgttcca cttttggtag
    16261 tgaatttcgt cgtcgctatt cagaagcagc tcgccgcctt cctggatata aacaggggta
    16321 accgttttgc cagtgacgaa attgtgtgcc tgcacacccg gggctaagag catgataatc
    16381 aacgcaataa tcaaacgaga tgacatcatt gctccggcta tagcggaaaa cgcgaaatca
    16441 gcagctctaa gcgctgacaa atatcgctat ccagcgccaa cggtgcataa tacgactggc
    16501 ggttttccat atcatccgcc accatgtcgt cgtgtttatc tcccatcagg ccattaatgg
    16561 cggcgaggaa acgggaaatc tgtaacgcct ctttattttc aggatcaagc gatcttaagg
    16621 tgtcgtaata agaaacctgc tggctgatga tttttcgcac atcatgaaat tccctgccgg
    16681 ttagtcgcgg tttggcaata atctctttca gggtatgcat ttgcttttgc cgataggcaa
    16741 taaaactgct tacgctttct aactggctat ggcgtaatga atagttcacc aacgaccaga
    16801 ccggagagct taaataaatc cgtaacaggt tgccgtaata cgacacaata tttttcttac
    16861 cgttacgaaa gccgtcctga aaacgcccca gaaaaacggt cgttttgccg aataaaaatc
    16921 cggcctgatg cttctttaaa tacagccgtt gcgcaaaacg caggtgcttg tagcgcgctc
    16981 gcatgtattt aaattgtttg cgctcatcag cagtcagctc atcgcctttg gcttgtttgc
    17041 gaactaaacg cagtaattcc tgacgatcaa cgccattgag ccagaattgc aggcataacg
    17101 cgtaattatc atgaatgatt tcggtggtac aacttagtgc aatagtcggc ggcaaactaa
    17161 cctgtgcatc aacgacatca tcttcttcaa tcgcaacaaa tagacgctcg ctcgcctcaa
    17221 gcgggaaata ttttctcatc atcaggattt ctctccggct gcgttataaa gcaaggaatg
    17281 gcgctgaaaa aatgccttta aggttggaat taacgagagc gccacgtcca gacattgccc
    17341 accgaagtaa gcaaacgctg ctcccagcac accaaacaga tgacagaaca tgaacagcaa
    17401 tacgatgtag ccgattgagg cgatggtttg cgccacgaga aaagcgcgct gttttcccgc
    17461 catcaatagc aatgactcct gcgggaagcc cagcatcgaa atcacaattg cgcccagcat
    17521 cacctgaatc aggtcatacg cttcgagata cttaacgcca aacaccagcg aaatgagcgg
    17581 cttgccgacg atcaacaccg cgagcgccac cagaataccg attccgcccg ccagtaaacc
    17641 ggacttcaca cctaacaacc acggtctggt ggtgcgcgga tctaaacgca tcacctctgg
    17701 gtaaaaactt ttacccagca aacccgctgg cgttccggcg gcgtcgaaga atgtcatggc
    17761 gattttaaat aacccagcgg cagcgggtcc taacacgatc cccaccaaca ctgtgctgca
    17821 cgagttacgc gccgaccaga tggagtgggc aatgttggtt gaccagacaa aactccacgc
    17881 gcctttaatg tgtcgggcag actcaaacag attcaatttg aaggcgttat ggatatttct
    17941 acggcgtaat tcgcgcgcgg caaaccacca gtacatggtg ccgccaacca gattcgacac
    18001 gtaccaggca ataacaaaac ccgcaaaacc aaagtcaaaa taccaggctg cgacgctccc
    18061 cgctgcgcgc agaaaaggtt tcgtcgcctg ctgtacagca atcaaatcaa agcgatctac
    18121 cgcacgcaga atgccggtcg gcgtggagga agccattgaa ggaatgagcg tgcaatacag
    18181 cgccgccagc caaaagcttt ggtcatccag acctaatgaa tgggaaagga atggcagtaa
    18241 ggcaatgccg ccgacaatcg ccaccgcgcc gctgacgata tccagcgaga aggaaaatga
    18301 gacgacattg cgaaattgct gcggattatt gttggtcaag gctggcgttc cgtactgaac
    18361 caccagctgc catgtctgaa acttaataaa atcgctgatc gacttggcgt acgattgcac
    18421 aatcaccagt acaccaaaca tggcgggcgt catcccttta ccggcacacg agagcgccaa
    18481 cagccccagc aaggcgctct cgacattact ggagcctaac caggcgctat tgcgaataat
    18541 ggtgcgaaac gcgccatctg caaaccaatg tttgatgtta aaaccagcca attcagcctg
    18601 accttatcgt tgatagtaaa aaatatcccg ccagccttaa gttaaacttc ggcggtcaga
    18661 aacgatggca accagagaaa ccgccttctg tacctgttcc agcacttcgc tgtagggcgc
    18721 tctggaatca atctcaagaa tttttgtgcc gttatagcca atcgtcgaca tgacgccgat
    18781 tttgtcctgc agctcggcat actcatggtc aggcttgcgg gagatggcag tatcaatatc
    18841 aatccccaaa cgaatgatta attcaggacg gtattgcgcc attttttggt ataaactccg
    18901 ttcgcgctgc gccagaaaca tgctgatttt cccggtcgca cgttcgacgc cgatccccgg
    18961 tccgtcataa taaaagcccg aaatttcagc ctgcgggaag cgatcgctga ccaccagaac
    19021 gccactttgc gccagtcgct gaaccttgcg tagattcgcc attcttcgca gcgagaagca
    19081 gtacataatc accgccgccc atagcgccgg agatttggtt ttcatgcttt gggttttcga
    19141 ggatttggcc gccagtcgcc gttccagcca gacgccaacc aacggcaatc gtttgatttt
    19201 gtcgccgtct tcgccggaga gcagccccag atagcggcgc tcggtttgcc agtgttgttg
    19261 cagcgatttc accaggtcgg tggtcagcgt ggatttaccg gtgccatcac acccgaccac
    19321 cgcaatcagc cccggaatgt agttgggctg cggcgcggtt gtactattaa cagtttgtgt
    19381 ttgtagtgca tccattcgtt aaatcatccc ctatccattt tggtgatcag gaaagcgcgg
    19441 cgtgaattgc gcgtagtgac gcttgcataa tttcatcggc tagttgccga ccatccagtt
    19501 caaggatctt ggcgccattg aatgtcagtt gcggcgtaac ggcgattttt tcctgtaacg
    19561 ctgccagttg gtggtcaggt ttacgcgcaa acgcggtttg ttcatcaatg ccaagacgaa
    19621 tcaacaatac gggtaaataa gacgccatcc attggtacag cttcaactcg cgctgcctta
    19681 acatttttat ccaaccgtta ccgcccgtgg tttttgccaa ttgcgggcca tcaaagcgaa
    19741 aacccggcac ttcaacctgc gggtagcggt cggtgatgag cagatagcct tgctggcttt
    19801 tacacaacat tttgcgaaac ttgtacgccc gccagcagga aagcagatag ataaccagcg
    19861 cagtaatatt gccaggcggt gttgagggct tttcgtgtac atgtgccgct ttacttcgca
    19921 gataacaccc acaaggtgcg ccaataacag ggagctgtga aatccattcg ccaattcgcc
    19981 cggacgattg cccgagataa atgtgttctg ttggcattct tgctgccagt tcatttacca
    20041 ggcttgccgt gagggtcgat ttacctgaac catcacatcc tacaatggca ataacacgca
    20101 ctggcgtgga attaattatt gacatattac gttgattcac gaaaaacccg gcagtaaata
    20161 atgtattgaa tattacgtgg tccgtgctca gaatatccgt tcaaccttat tgtgacaatg
    20221 gttaattgtg acattgacct gaattacgct ttcataaaaa catattaacc aaataaatat
    20281 ttttaatgga tatttaaatt aaaggatata ttcatgcagt caataacacc tccattaatt
    20341 gccgttattg gtagcgatgg ttcaggcaag tcaacggtgt gtgaacatct tattaccgtt
    20401 gtcgaaaaat atggtgctgc cgaaagagtt catttaggaa aacaggccgg aaatgtcggt
    20461 cgtgcagtga caaaattacc tttgatggga aaatccttac ataaaacaat tgaacgaaat
    20521 caggtgaaaa cagcaaaaaa attgcctgga ccagttccgg cgctggtaat tacagcgttt
    20581 gtcgcccgtc gcttactgcg ctttcgtcat atgcttgcct gtcgtcgtcg cgggttaatt
    20641 gttctaaccg accgttatcc tcaggaccaa attcctggcg cttacgatgg tacggtgttc
    20701 ccacctaacg ttgaaggtgg tcgttttgtc tcatggctgg caagccagga acgtaaagcg
    20761 tttcactgga tggcgagcca taagcctgat ctggtcatca aactcaatgt tgaccttgaa
    20821 gttgcctgtg cacgtaaacc cgaccataaa cgggaatcgc tggcgaggaa gattgccata
    20881 acgccacagt taacctttgg tggtgcacaa ctggttgata tcgatgccaa tcagcctctg
    20941 gaacaggtgt tggttgatgc agaaaaagcg attacggatt ttatgaccgc gcgtggttat
    21001 cactagtcaa aaatggaaat gcccgatcgc caggaccggg cattttcagg aaggttaaat
    21061 caactgcaat gctatccagt acagcccacc agaaagaaaa attgccgccg gtaaagtaaa
    21121 cacccacgcc atcaggatgc tggttaccgt tttacgctgt aacccaccgc cgtccaccac
    21181 catcgtccct gctaccgcag acgagaggac gtgtgttgtg gagacaggca tcccaatata
    21241 actggcaagg ccgatagaca ctgccgccgt catttgtgcc gccatgcctt gcgcatacgt
    21301 catgccgcgc ttaccaatct tctcaccgat ggtcatcgct acacgacgcc agccaatcat
    21361 ggtgccaatg ccgagcgcca gtgctaccgc catgatgatc cacaccggag cgtactcaat
    21421 ggtgcttaac atatcgctgc gcagtttctt cagcaggttc tggtcttctt tactgacgcc
    21481 tggcagtttc gccagcttcg cggaggtatc agagatgcac agcataatgc ggcgcagctg
    21541 gctgcgctga ctcacgctta acggctcgta actttccata ttgcctggca gcatcgtttt
    21601 aacgcgcgca atcgcatcaa aggtatttgc cggatgacag tgaaactctg ttacttgcgt
    21661 gccatcagtc gatgctgcag gcaatggagg ttccatggca atcaacttct gcggcagttc
    21721 aggatgctgt tgcaggtagt gttcgaagtt ggtaacggca tcgcgggtac gggtaatttc
    21781 atagccggac gcattcatat tgacgacgaa gccagcaggg gcaatcccca ccagtaccag
    21841 cattaccagg ccgatccctt tctgcccgtc gttcgcgccg tgcgaaaacg ccacgcctgc
    21901 agcggaaaca atcagcgcaa tacgcgtcca gaatggcggt ttacgtttgc cctttttctt
    21961 tttgcgatct tccggaatgc ggtgaatacg gtcacgcttt ttcgtcccac tccagaaacg
    22021 gcgcagcagg aaaatcagac cacccgcaat gaccaggccg acgatagggg aaacaatcag
    22081 cgaggagaaa attttggtta cttcacgcag gtttaacgca tccatcaccg atgagccggt
    22141 taacagcgcg ttggttaaac cgatgccgat aatcgcacca atcaaggtgt gcgaactgga
    22201 ggccggtaaa ccaaagaacc acgttcccag gttccagata atcgccgcca gcagcatgga
    22261 aaagaccatc gccagaccgt gagttgaccc catattcagc aacaaatcgg ttggtaacat
    22321 atggacaatg gcataggcaa cgctaagtcc gcccaataac acgccaaaaa agttaaaaaa
    22381 tgccgccatc accacagcaa gttgtggctg catggcacga gtataaataa cggttgccac
    22441 cgcattcgcc gtgtcatgaa aaccattgat tgcttcgtag aacaacacaa atgccagagc
    22501 aagtaataac aaaagccccg tatatatatc aaggccaaca aataaattta gcatatatta
    22561 gattacgcca ttttgaattt acgaacggac gcattatcag tgactttaac ggcatgggca
    22621 aagtgaaata tcatttttta acgataaaaa gagtgttatt tcttgtgtat tagacaataa
    22681 gcattcattc aaaaccttta gaaataatca aaacacaggt attccactgg ttaatatatt
    22741 cacatatgaa atgaataaat atttgaatat ataaatattg aatattttga ttaatccccg
    22801 tactaattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
    22861 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
    22921 cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt taccgtccac
    22981 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
    23041 tttgtccagc acctcttcat gatggccgac caggtcgata ttgctaccac agcgaccggc
    23101 gatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
    23161 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
    23221 ggggatcacc gtccacagcg gctcaaagac cagcacttcc gggcgcagca atacgtcgat
    23281 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
    23341 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
    23401 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
    23461 cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
    23521 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
    23581 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
    23641 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
    23701 ggagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
    23761 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
    23821 tttcggaatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
    23881 aagatacatc agatgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
    23941 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gcacataggc
    24001 gttttgtagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
    24061 acgcgctcca ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
    24121 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttaag
    24181 ggtatagcag ccgttttcga ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg
    24241 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
    24301 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
    24361 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
    24421 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
    24481 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
    24541 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
    24601 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccgcctgggg cgtagcccaa
    24661 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
    24721 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
    24781 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
    24841 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
    24901 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
    24961 gctggcggcg cgttcgccaa tatcaatcgc ccgttttctg gtccgacgca tgaaaaaacg
    25021 ctgcccgttg gcaaacatcc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
    25081 gtaacgatta tgcttgagga gctactggcg ctgggcgtta ctggtgcaga gtacgacgcc
    25141 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
    25201 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
    25261 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatctg
    25321 gcaaaacgga ctgaaacgtt gaactggctg ttctggttac agggtgccgc gccgttcctc
    25381 ggcggtggtt ttggtcactt ttaccattac gcgccggtga aaattgagta cgccatcaac
    25441 cgctttacca tggaagccaa gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat
    25501 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
    25561 aacgtggtgc tcggcggcgt gtatgatgcc gcagagtttc tggatgcggg aagttataaa
    25621 catgtgcaac gctgggcgaa agaagtgggc gaacgtccgg cagtgaaacg tgggcgtatt
    25681 gttaaccgca ccaacgggcc gctgaacgag cagttgcatg agcgccatga cgccagtgat
    25741 tttgagacaa atacagaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
    25801 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
    25861 acgctgccgc gtcttatcag gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa
    25921 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
    25981 gcaaatccga cgtgtaccag cacccactgg cccagcagat cggcagggtt accttcgcaa
    26041 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
    26101 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt taactccggt
    26161 tatttcgatt gccggacaaa cacgaaaaag aacctttagc gggtgttccc tctcacccta
    26221 accctctccc cggaggggcg aggggaccgt ccgtgcgctt tttaggcgtt gtcctcagtc
    26281 ttattcaact tcaatacttt tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc
    26341 gccgtgacag agcggacact gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc
    26401 ccagcaccag gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt
    26461 tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc aggagagcgc
    26521 gccaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg cctgccgttg
    26581 gataatttca acggcgctct ggcaaagcga caactcatgc attttcgccc ctcctgcggc
    26641 caaacagcag ggcgcgacgc cctgcgtgtg gtacatccgg attggtgact ggcaacgaaa
    26701 ggatcattcg tgcgcagtca tccgtcaggc gttggccttc ttcaatcgac atgctgtgcg
    26761 aaagcggcga catcagcgaa caggagaggt attgcgaaac accgtccagt tcgccaacgg
    26821 taaaggtcat ggtgccatac ggcagttgca gaccaatttt ttcactcact ttgcgcagcg
    26881 gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg gtgatcacac
    26941 accccgtcca ctgaccttcg aacagcgtaa aatcagaaac atacaccggc attgaaggat
    27001 gcagaaaaga gagatcgtgc atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt
    27061 gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc ctcccgtggg
    27121 atggcttcca cgcctgattc gcgcagcgca gccagaacca gctcaagcgc aggttcaatc
    27181 attgcttcaa ccgtcggcgt taagccgatg tgtggctcca gcgattccgg gatcacgccg
    27241 accagggtca gctttttcgg aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc
    27301 aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc ttcatcccgc
    27361 aggatcatca tcgttcccgg cgtgttcttt ttcgacacaa tggcatccgc aataatcaga
    27421 tgatcgcgat ttgccatgtc gctaagcagc tccattcccg ccgtgccgcc atcgaggatc
    27481 tcaacataat ccggcagaat gtatcgttgc tctaacgctt cgacaatccg cacgccgatg
    27541 gcttcatcgg tcagcaaaat attgccgacc cctaagacta aaatacgcat tacagaacct
    27601 tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag gccatgcacg
    27661 ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg gcaaccggtg
    27721 tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc ggaccagagt
    27781 tccaggttga tggaacaacc gcctggtagt tgctgatgat accgtcttta ataaccatcc
    27841 agtgagagag cataccgcgc ggcgcttcga ggaagccaac acctttgaac tcacccgttg
    27901 ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg gtgatcagtg
    27961 cactgtattg gttttgcagg atatcctgca attcacagca gtgaacggta cgaccaataa
    28021 tacggcccag cgtggagtgc agctgtgcca cttccagcgt gttgccagtc agtttctgat
    28081 aaatcgcaac gatttcattc agtttgttct gggtagattc gcgacctgcc gccagtttca
    28141 ccagcatatt agccagcgga cccacttcta ccgttttgcc gtagaaagtc ggtgatttca
    28201 cccaggaata tttcccgtcg tcagaccaac catcataagc cggaatggtg gtaccttccc
    28261 acggcgcctg tggcgcttcg tctttatacc aggagtgctt cgcgctttcc tgaatgcctt
    28321 tgatcaggta ttcatcggaa tgagaagtga tcggacgata cgaggacaga tccgcattct
    28381 caatgtagcc gcccgggaac aggaagctgc cgtttttgct gtcggtcggg aattccggca
    28441 cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag aacgcggcga
    28501 taaccgcggt atcaacctta taaacctgct caacaaagtc gctcagtttg tcgatgaaag
    28561 acttgatgta catcaggcgc tcaaggttca acacgcccaa cccgtcgagg ttgattgggt
    28621 tcgcgacacc actcactgcc aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca
    28681 cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct accgcaatca
    28741 ggttcacttc cggcggcagt ttcatcgccg ggtgacccca gtagccattg gcgaaaatac
    28801 ccaactgacc gctggcaacc agatctttga tcttgttctg aactttagtg aactcttccg
    28861 gactgttcag atgccaggtc gaaacgcctt tcagcatttc ggaggctttg gttgggtcag
    28921 cttgcagtgc agaagtgatg tccacccagt ccagcgccga aagctgatag aaatgaacga
    28981 tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc gcgttaaccg
    29041 gaacgtcgat attcagcgca ctttctgccg cacgaacgga agacagcgcg tgagtggtag
    29101 tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg cggtttttca
    29161 cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa acgacgccat
    29221 tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa
    29281 ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt ttaaaggagg
    29341 aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc
    29401 aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa
    29461 tgccaccaac gaataggtca gacgccaggt tgcacaacct aacagtgcgc tcagtgctga
    29521 caggaacagc atgcgggaat cattacgcag cttcgccaca cgcagaacga ccagcgggaa
    29581 gagcatcagc aggacttcaa tccagaacat cacggagtag aagtcaccgg caaacgctaa
    29641 cgacagcttg tcgcgataga taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa
    29701 cacactgatg gtgttggtca gcttgacgaa caggctcttt tcatccggac cgttgccacg
    29761 cagacccgcc tgcaccagtg aaccttcaaa gatgacaatc gagaagccca tgatgaacgc
    29821 cgtcagcagc gagaacagcg gcaacatttc atagctctgc cagagcggat gcaccttgta
    29881 gcccgccgag atcatcagcg accccattga agactggtgc atggtcggca gcagcgcacc
    29941 gagcgcgatg atgaagaaca tcaccttgtt cagacgttgt agcgacacct tccagcccag
    30001 acgttcaaac agtgccggag caaactccag agccatcacg ccgatgtaga tggtcataca
    30061 gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt agaagtacgg
    30121 caggttccag tagcgaccca cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa
    30181 cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt taaagacgta
    30241 taccgcccac gccagcgccc agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa
    30301 cgcgatccac acgccccacg ggaagccgcc gttcaggtca gagacagagc ccagaccgaa
    30361 caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa aaatcatgac
    30421 cggtttactg atgattttgc cacccagcgg ttgtggatca tgactcatga tcgtctcctc
    30481 cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac ggttaagccc gccagcacag
    30541 ccagtggtag catcatgcct ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg
    30601 tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc gtcagtacca
    30661 gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat ttcggcaccg
    30721 tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat tcgctgccag
    30781 gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca
    30841 ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatcg agacgttcca
    30901 cacctttctg gttgcacagc tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt
    30961 acttcggcac gttgtacgga caggcgacca tgcagtaacg gcaaccggtg cacacgtctt
    31021 tgtcgtaatg gacaatgccg gttttcggat cttttttcag tgcagagacc gggcacacag
    31081 agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg tagccgttct
    31141 cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg ttattggtat
    31201 acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga
    31261 agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt
    31321 acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca tgactgacag
    31381 acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa ttacgtctgt
    31441 tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac ccagttcacg
    31501 caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca aaccaatagc
    31561 gcctgcagag acgttgccgc cctctttagc gttaacatcc ggtttctgtg aacgcggagt
    31621 ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac ctttttcgtt
    31681 acagccatag cacgggtgac caatcgccac cggccacaca ccgccaacat cgcagaattg
    31741 cagcgttgag cagttgccgt aagtttctgg ccctttacag ccgaggtggt acaggcacca
    31801 gccttcgcgg tggccttcat caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg
    31861 gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac ggtttttgtc
    31921 atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg
    31981 cgggttcggc gggcagcccg gaatattgat aacggttttg cctggcagaa cttcttgcaa
    32041 gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg cggagcagga
    32101 accaatagcg ataatggctg ctgcgccttc cgccgctttg cggatgtgat ccacaatcgg
    32161 ctcaccggca accatgcaat aaataccgtt atcttttaat gggatggaac catccaccac
    32221 taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt cgacctgatg
    32281 accgaaggcg gcggaaagca cttcgtgata ttccagagag atagtctcca gcacgaggtt
    32341 ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc actcctgcgc
    32401 gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca tctctgcagc
    32461 ggctttgcta cttaacccca tggtggcggc taatgctaca caaagcttca tgaaatcacg
    32521 acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata gttattccgt
    32581 tgcgaggacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc
    32641 atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg cgctaaaacg
    32701 aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt tgatcaattt
    32761 tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag
    32821 aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc cgcttcccag
    32881 gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc taatacaaaa
    32941 ccgcgtttat aatcggcaca gaattttgtc acattaaccg cactgtcggc atgcgttgct
    33001 tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcaccc cattcgttat
    33061 atatcgccat atatagcgat attttaggca aagaacgact tttgcgcacg ctattttttc
    33121 atacttgttt aagacgtaat cccgtaagag ataaacctat caggagagat atttatcgaa
    33181 ccatttcagt gtcctttgcc aggcaagatc ggcggcagat ttgtcataac ggggcgtgga
    33241 atcattatgg aatccgtgat taacccccgg atagatataa gcctcataaa ccttattatt
    33301 gtctttcaac gccgcctcgt aaggaggcca gccctcgttg attcgggtgt ccagttccgc
    33361 gaagtggagt agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg cctgacgacc
    33421 ataaaacggc accgcgcagg ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc
    33481 accgccatag caaaatccgg taatacccac tttacctgtc gcttgcggat agcgttgcat
    33541 aaactcaatt gcggcaaaga aatcattcat cagtttggtt ggatcaacct gctgttgcag
    33601 ctcacgacct ttatcatcat ttcccggata acctccaacg gaacttaatc cgtcaggtgc
    33661 cagggcgata taccccgcct tcgccactcg ccgtgccaca tcttcgatat acggattcag
    33721 tccacgattc tcatgcacca cccccacggc tggcgttttg ccgctcattt ttgcgggttt
    33781 caccagataa ccccgtacct cgccgtgacc atttggcgaa ggatacgtga tgtactcagc
    33841 aacaatctcc gggtcggtaa actctacctg agtcgccagc gcataatttg gcttgagcaa
    33901 atcaaacaac gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa
    33961 ctcacgtttc gaaattttcc cgtgaacgta atagtcgtag taatccaaca actcttgtgg
    34021 gaaatctttg gcggttaaac gcggcatcgt tgcattcctc aactggtgtt tttttaagca
    34081 aagcataagc gcgtgttttt gcccagtttt tcgtcactct gtgagccaga ctacgggata
    34141 cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag
    34201 gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga
    34261 aaaagtggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt
    34321 aacgcgctgg aatcacagcg tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac
    34381 tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc
    34441 ctgctgcgcg aggattttgc tgcttatcgc gatgaactga ttatctctac caaggctggc
    34501 tacgatatgt ggcccggccc gtacggctct ggcggttcac gtaaatacct gctcgccagc
    34561 ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta ctctcatcgc
    34621 gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc
    34681 ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca aaaaatggtc
    34741 gagttgctgc acgagtggaa aattccgctt ttaattcatc aaccttcgta caatttactg
    34801 aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg cgtgggctgt
    34861 attgccttta ctcctctggc tcagggattg ctgaccggaa aatatctcaa cggcattccg
    34921 caagattcac ggatgcatcg tgaagggaat aaaattcgtg gtctgacgcc gaaaatgctt
    34981 accgaagcca acctcaacag cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa
    35041 tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg atcgcgtgac gtcggtattg
    35101 attggtgcca gccgcgcgga gcaactagag gagaacgtgc aggcgctgaa taatctgaca
    35161 tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggcga actgaatctg
    35221 tggcaggcgt cgtccgataa atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc
    35281 agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg cagaaagcac
    35341 aaacgtcaga tggataatga cgtaccacat cagtttgtta tccgggacat ttttcgcatc
    35401 cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg ctactttgtt
    35461 tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt tctcggagat
    35521 atccagctgt gagacgaaat tctcataacc ggaaaacatc accatcacca gcaaaccgcc
    35581 aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt ccgccatcga
    35641 gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca gggcaactaa
    35701 cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat acattgcatt
    35761 ttcaaaaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca gtatatcgca
    35821 attgagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg tcgcataatt
    35881 ctgaggttta gggatcttcc tggttccctt atcaacgcat cgagagcgtc tacccttaaa
    35941 ggacgttgaa cctgaaggga gaaaaacgat gtctcattta aaatgacccg accacgcagt
    36001 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    36061 caccgatacc ggattgcggc gagaaaacct atgttggtag cgctcgcctg aaagatcgta
    36121 aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc
    36181 gagaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    36241 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    36301 atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata
    36361 ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg
    36421 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    36481 cgatccccct gctaccgaaa ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc
    36541 agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc
    36601 gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    36661 ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    36721 gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt
    36781 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    36841 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    36901 ctcttacctg atgtggcgaa aacgccttat ccgcctacaa acgcatgaag attcaacatg
    36961 ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc acctaatgcc
    37021 ggatgcgtcg tgaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat
    37081 agattttgta ggcctgatga gcgtagcgta tcaggcaatt ctactttact tcgctttggc
    37141 ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac
    37201 cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt
    37261 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    37321 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    37381 ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc
    37441 cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat
    37501 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    37561 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    37621 caggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa
    37681 gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg ctgccacgag
    37741 gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt
    37801 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    37861 accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    37921 acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    37981 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    38041 gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgtgc
    38101 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct
    38161 cttactgaag aagattgccc aggtgactac ggaggccaaa ataagcccaa tcatcacgca
    38221 cttaacgaca atatcggcgt gctgatacat accccagacg gaaaggtccg tctgcattaa
    38281 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    38341 aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    38401 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    38461 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    38521 cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg
    38581 caggacgcag caaaaaatac actcttggcg cggtaaatag cgtgattcag cgcgcttctt
    38641 cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg
    38701 agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg
    38761 aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt
    38821 cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    38881 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    38941 atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc
    39001 tggaatcgcc gggttccatc actatggaag tccacgacgt tccggcgatt gttgccgccg
    39061 tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc
    39121 tgtttaaggc gctggatttt ggcatcgatg tttctattca ggccgccacc aaatatctgg
    39181 ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc
    39241 tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    39301 gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag
    39361 tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct gccctgcctg
    39421 gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct
    39481 ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat aacttcagtt
    39541 tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca aatcaaccag
    39601 aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc ttgattcgcc
    39661 tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcac
    39721 ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt
    39781 aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat aggcagataa
    39841 aagcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    39901 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    39961 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    40021 gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga ttttctgcct
    40081 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg
    40141 cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    40201 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    40261 tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac
    40321 cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt
    40381 tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    40441 ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg
    40501 gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc tgctgtaccg
    40561 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    40621 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat
    40681 cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg
    40741 gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa
    40801 cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    40861 tttattctca attcgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac
    40921 cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    40981 gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc
    41041 cgccgcgaat aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga
    41101 cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc
    41161 gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    41221 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcg
//
LOCUS       DADLTI010000012        41031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLTI010000012 DADLTI010000000
VERSION     DADLTI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.012"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..221)
                     /locus_tag="JN755_000646"
     CDS             complement(<1..221)
                     /locus_tag="JN755_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HAY7379546.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIG"
     gene            complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JN755_000647"
                     /pseudo
     CDS             complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JN755_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1532..1777
                     /gene="zapB"
                     /locus_tag="JN755_000648"
     CDS             1532..1777
                     /gene="zapB"
                     /locus_tag="JN755_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7379547.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JN755_000649"
     CDS             complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JN755_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7379548.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JN755_000650"
     CDS             complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JN755_000650"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7379549.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JN755_000651"
     CDS             complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JN755_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7379550.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JN755_000652"
     CDS             complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JN755_000652"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7379551.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JN755_000653"
     CDS             complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JN755_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7379552.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JN755_000654"
     CDS             complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JN755_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7379553.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JN755_000655"
     CDS             complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JN755_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7379554.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10030..10242
                     /gene="rpmE"
                     /locus_tag="JN755_000656"
     CDS             10030..10242
                     /gene="rpmE"
                     /locus_tag="JN755_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7379555.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10303..10911)
                     /locus_tag="JN755_000657"
     CDS             complement(10303..10911)
                     /locus_tag="JN755_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7379556.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JN755_000658"
     CDS             complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JN755_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7379557.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11689..12849
                     /gene="metB"
                     /locus_tag="JN755_000659"
     CDS             11689..12849
                     /gene="metB"
                     /locus_tag="JN755_000659"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7379558.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12852..15284
                     /locus_tag="JN755_000660"
     CDS             12852..15284
                     /locus_tag="JN755_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7379559.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15633..16523
                     /gene="metF"
                     /locus_tag="JN755_000661"
     CDS             15633..16523
                     /gene="metF"
                     /locus_tag="JN755_000661"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7379560.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16852..19032
                     /gene="katG"
                     /locus_tag="JN755_000662"
     CDS             16852..19032
                     /gene="katG"
                     /locus_tag="JN755_000662"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7379561.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19093..20031
                     /gene="yijE"
                     /locus_tag="JN755_000663"
     CDS             19093..20031
                     /gene="yijE"
                     /locus_tag="JN755_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7379562.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20058..20675)
                     /locus_tag="JN755_000664"
     CDS             complement(20058..20675)
                     /locus_tag="JN755_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7379563.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JN755_000665"
                     /pseudo
     CDS             complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JN755_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JN755_000666"
     CDS             complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JN755_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7379564.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JN755_000667"
     CDS             complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JN755_000667"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7379565.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25537..26616
                     /locus_tag="JN755_000668"
     CDS             25537..26616
                     /locus_tag="JN755_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7379566.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26631..26951
                     /locus_tag="JN755_000669"
     CDS             26631..26951
                     /locus_tag="JN755_000669"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7379567.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27002..29299
                     /locus_tag="JN755_000670"
     CDS             27002..29299
                     /locus_tag="JN755_000670"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7379568.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29265..30143
                     /locus_tag="JN755_000671"
     CDS             29265..30143
                     /locus_tag="JN755_000671"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7379569.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30145..30486
                     /locus_tag="JN755_000672"
     CDS             30145..30486
                     /locus_tag="JN755_000672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7379570.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30473..31324)
                     /locus_tag="JN755_000673"
     CDS             complement(30473..31324)
                     /locus_tag="JN755_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7379571.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JN755_000674"
     CDS             complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JN755_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7379572.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JN755_000675"
     CDS             complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JN755_000675"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7379573.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JN755_000676"
     CDS             complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JN755_000676"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7379574.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37697..38701
                     /gene="argC"
                     /locus_tag="JN755_000677"
     CDS             37697..38701
                     /gene="argC"
                     /locus_tag="JN755_000677"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7379575.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38709..39485
                     /gene="argB"
                     /locus_tag="JN755_000678"
     CDS             38709..39485
                     /gene="argB"
                     /locus_tag="JN755_000678"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7379576.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39546..40919
                     /gene="argH"
                     /locus_tag="JN755_000679"
     CDS             39546..40919
                     /gene="argH"
                     /locus_tag="JN755_000679"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7379577.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9342 a        10532 c        11258 g         9899 t
ORIGIN      
        1 ataccgatag ctgcaatttg atcggaactg atatcggctt tcgccagcac ttctaccagc
       61 gtggagcttt gggtggccca gatttccatt gggtcgtgtt ctacccaacc tggttttggg
      121 tagatttgct caaattcgcg ctgcgacacg ctaatgatat tggcatcgtg atccattacg
      181 accgcgcggg agctggtggt gccctggtcg agcgcaacga tatatttttt tcagtcatgt
      241 ttaattgtcc cgtagtcata ttacagcgaa gctttttgtt ctgaaggagt tgtggtttcc
      301 ttttcttcca caacacagat atcgcaaggc aaatggcgac caatcagttt gcggtaggca
      361 aatgcaccta caatcgcgcc aacgataggg ccgaaaagcg gcaccaggaa gtaaggaatg
      421 tctctgccgc cggtaaaggc gacattgccc cagcccgcca gctaggcaaa gactttcgga
      481 ccgaagtcac gcgctgggtt catggcaaaa cctgtcaatg ggcccataga tgcgccaatg
      541 accgcaatca gtagaccaat cagcaaggga gccaaagggc cgcgtggtac accgttgcca
      601 tcgtccgtta acgccaggat aagccccatc agaatagcgg taatcaccat ctcaactgcg
      661 aaagcctgca caaaattgat atgaggatta gggtaagtag agaaagtgcc agccagatca
      721 acactttcaa cgctgccgcg aacaatgtga tgagtctgct cgaagtcgaa aaataaattg
      781 tagtaaagcc cgtaaactaa agccgcagcg cagaaagcgc cggcaacttg tgaaacgata
      841 aaaggaataa ctttgcgctt gtcgaaacag gcaaacagcc acaatgcaat ggtaacagcg
      901 ggattaagat gcgcgccgga aacccctgcg gtcaggtaga tggccattgc cacccccagt
      961 ccccaaatga cactgatttc ccactgacca aaagacgcac cagcgacttt tagtgctgca
     1021 acgcaaccca caccgaaaaa aatcaacaac ccggtaccga ggaattcagc aatgcactgg
     1081 cctttcaagg ttgatgtttg actcataatc ggatcctgaa gagttaatgt ttgttgtatg
     1141 tgtgaaagtc acggacctcc acgatgcttg taggcatgct gttaacttat cgttaacgag
     1201 caaaaacgag aaatatcgaa cttaaaatgt gtgtgcttcg tcataaaatg agcgttatcg
     1261 cgccatttat tccatctgat tgtacatgat gcatgtttgt tagatcattc gcgcaacaaa
     1321 tttattaaca ttttcgattg atgtggatta tgtcactagg agaaggatga aaagtgtggc
     1381 aaaccgtaat ctgcaaggcg tgccgctgga cagcgatggc gcggcttcat acaatcggag
     1441 ctaactaaag tgcgctcgta tttattaagg cgtcaccggt aatcgggacg aggatttttt
     1501 atccatcaac gccttgcaat tcaggagagg tatgacaatg tcattagaag tgtttgagaa
     1561 actggaagca aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga
     1621 agagctgaaa gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg
     1681 cgaagagctg gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg
     1741 tctgcaggcc ctgctgggtc gcatggaaga ggtctgatta cgcttcatct ttcgcgtaat
     1801 ttctttactg tgtaggccaa caggtaacgc agaaaaaagg caccttgcgg tgcctttctt
     1861 atcattcaat atccagcgga tcttctgaaa gaataatccc ggtattgtcg gcataaagat
     1921 ggtcgccgga gaagaaggtg acaccgccaa aattgacgcg gacatcgctt tcgccaatgc
     1981 cttcgccagc ggcaccaacc ggaattgccg ccatcgcctg gatgccgata tccaactctt
     2041 ccaggtcatc tacctgacgc accgcgccgt aaatgaccag accttcccat tcattttgta
     2101 ctgccagacg cgccagttca gcatcgacca gtgcgcgacg aacagaacca ccgccatcga
     2161 cgacaagaac acgaccacgg ccattctgtt cgagcagatc gtacagcaac ccgttgtcct
     2221 cgaaacattt taccgtgatt atttgtccgc caaacgacgc ccgtccgcca aagttggaga
     2281 acagcggttc cacgacgtta acatcttctt gatagatgtc acaaagctcg gaagtatcgt
     2341 atttcatagg cttaacattc agttgctgcg agaattttca gtatatcgcg ctatgtgggc
     2401 tgttggcaaa gtcatcaatt gttaattgat atttgtcagt tatgctgccc actggcttag
     2461 gaatatccct aaaacaaaca gcaggttagt cagtaacgct cccttgacag tacgttccag
     2521 cattggtcgc atcgccaccg ggtccatttc ccgcatcaca taacgggctt gcttcaccag
     2581 taatggtgcc gccagcagga acagccagcc ccacaggcta tgcagcgaaa agagattaaa
     2641 cagcgccaga cacaccagcg agcccatcag caggcaggca tgataacgac gcgcgttcac
     2701 ttcacctaag cgcaccacca gcgtgttttt gccattttcg cggtcgctat tgatatcacg
     2761 caggttatta atattcagta ctgccgttgc cagcaggccg catgcggtcg ccggaaggat
     2821 cagtgccgga atcaatgtat gagcctgtaa ataccagctc cccatgacac tcaaccagcc
     2881 aaagaaaacc agtacggaaa tatcacccag accgatataa ccataaggac gattgcccac
     2941 ggtgtaggtg atagcggcaa tgatcgacaa cccgccaaga atcaggaaac cgacaaaatc
     3001 ggccagcgta tggcatgcca ctgcaaccag tgccagcccg gagagacaga tgagcacgac
     3061 ggtaataatg agcgcccgtt tcatcttttg ctgggtaatg accccttttt gcatgccgcg
     3121 tagcggccca atgcggtcag gtttatcgct gccttttacc gcatcgccgt aatcattggc
     3181 gaggttagaa aggatctgta atagcccggc ggtaattagt gccagcaggg cgaccagcgg
     3241 atcgaagtga ccttgccacc atgccagcgc cgtcccgacg ataattgcag caaaggcgag
     3301 ggggagggtt ttaggtcgta aactttccag ccacgcctga gttcggctaa tttgttgttc
     3361 agtcataata cgcgccaata aaaatggggc ctttcagccc catcaaacaa tgatgaaaat
     3421 gattgaacgc gattatagga taaaacggct cagatcttca tctgccacca acgcatccag
     3481 atgtttgctc acataatctg cgtcaatagt gatattttga ccgcttaaat cgctggcgtc
     3541 gtaggaaatc tcttccatta aacgctccag aacagtgtgt aaacgacgag caccgatgtt
     3601 ttcggtagat tcgttcacct gccatgccgc ttccgcgatg cgtttaatac cggagtcggt
     3661 aaactcgata tttacgcctt cagtcgccat cagtgctttg tactgcacgg tgatagaggc
     3721 attcggctcg gtcagaatac gctcgaagtc gctggtggtc agtgcctgca gttcaacgcg
     3781 gattggcagc cgaccttgca gttccgggat caggtcagac ggtttcgcaa tctggaacgc
     3841 gccagaagcg ataaacagaa tgtggtcagt tttgaccatc ccgtgtttgg tggaaacggt
     3901 gcaaccttct accagcggca gcaggtcacg ctgaacgcct tcacgagaaa catccggacc
     3961 ggaagactcg ccgcgcttac agattttgtc gatttcgtcg ataaacacga tcccgtgctg
     4021 ctcaacagcg tcgatagcgt cttgcttcag ctcttccggg ttcaccagtt tcgccgcttc
     4081 ttcttcaatc agcagcttca tggcgtcttt gattttcagc ttacgcgctt tttgcttctg
     4141 gccgcccagg ttctggaaca tggactgcag ctggctggtc atctcttcca tgcccggagg
     4201 agccataatt tcaacgccca tcggtgctgc ggcaagatcg atctcgattt ctttgtcatc
     4261 aagctggcct tcacgcagtt ttttgcggaa tgcctgacga gcagcggaag gttcctgctg
     4321 ctgttcagtc tgtccccagt tgtttttagc aggtgggatc agcacgtcga gaatacgttc
     4381 ttctgccagt tcttcagcgc gataacggtt tttctcgata gcctggacgc gtaccatttt
     4441 cacggcggca tcggtcagat cgcgaataat agaatcaact tccttaccga cgtagcccac
     4501 ttcggtaaat ttggtcgctt caactttgat gaacggcgca ttcgccagct tagccagacg
     4561 acgggcgatt tcagttttac cgacaccggt cgggccgatc atcaggatat ttttcggggt
     4621 cacttcatgg cgcagctctt cgttgagctg catgcgacgc cagcggttac gcagagcaat
     4681 cgccacagaa cgcttggcgt tgtcctggcc gatgatgtgc ttatccagtt cgctgacgat
     4741 ttcgcgtggg gtcatttcag acatgggaga tccttacgct ttgtagctta attcttcgat
     4801 ggtgtggaaa tggttggtat agatgcaaat gtcgcctgca atatccaacg ccttttcagc
     4861 aatttcacgg gcgctaagtt cagtgttttc taacagcgcg cgcgccgcag cctgggcgta
     4921 agggccgccg gagccgatag caataagatc gttttctggc tgcaccacgt caccgttacc
     4981 ggtgatgata agcgatgcag tttcatccgc gactgccagc agtgcttcaa gtttgcgcag
     5041 catgcgatcg gtacgccagt cttttgccag ctcaacggcg gctttaacca gatggccctg
     5101 atgcatttcc agtttacgtt caaacagttc gaacagcgta aaagcatccg cagtaccgcc
     5161 cgcaaagccc gcgatgactt tgtcgttgta cagacggcgg acctttttca cgttgccttt
     5221 cattacggta ttgcccaacg tggcctgacc atcaccagcg atgaccacat ggccgttacg
     5281 gcgtacgctt actatagttg tcacgagctg accccttggt tacgaataca gagtacaaac
     5341 cccgtacaaa agtacggggc ataatgcaat tatagatggg ggggattttg agggtttcaa
     5401 cccccggcgg cgagccgaat gcagtttgta tgacccgcca tcttcaaccg attgagggtg
     5461 ctgtctgcgt tctctttgcc tttcaccggg ccaatgacca cacgattcca gccattgttg
     5521 gtggtgattt tcgagtcaaa tccttcgaac gccagctgag cacgtaccgt ctctgcctgt
     5581 tccgcgcctc tgaacgaacc gcactgcacc atccagcggc gttcgtcttt tttctccgcc
     5641 gtcggttttg gcgcgtcagc ggcacgagca accggcgcag cttgctgcgg cttcgactgc
     5701 gcggtcgtgt gcgcaggggt ttgcagcaga tcctggtacg gctgctgagt agaagctggt
     5761 ttagactggc gcggttgcgc ctgtaccggc gcggcttgcg acgtacgcgt ctgctgctgc
     5821 cagctttgtt cagtcgtacg cgactgctga gccaggcgtt gctgctccgc taattgctgc
     5881 gcctgacgct ggcgttgtag cgtttgctga cgctgttccg gcgtctgttc attccacgga
     5941 acctcaacca gctgcgttgg ctgctggcgc atatcagcct gcatttgttc aagaagctga
     6001 cgttgttctg gtgtcagttg ctccggcgtt ttcacttcac caccggcaga aggttctgtg
     6061 ggcgcacgca cacccggctg gcgactttcc agctctttaa tgtagcgcca gcgttcttct
     6121 ggttttggtg gtagtccgtt tccggtcact ttctggcttt gcagcgtctc ggactcttct
     6181 ttcttgtgat gcgtaatgaa gtacagacca ccgataaagg tcacaagaac ggcggcagca
     6241 atagcgacca tagcgggaga aaccgcaggc agatttcgtt gctttttccg tgaggtgctc
     6301 ttttttcgcc gcgaaggtgc cggttggctg cggcgtacat aatctcgttg tgccactatc
     6361 gtttcgctgt atttattcgt tcgtcagccc gccatgttac ttaagcggcg ggcctttgac
     6421 cagtcacggc agtcttaaag gtttacttta aggtaacgcg cgtgttgatc cccggatgat
     6481 aagttcgcag tccattaaac gagagccact gccaacgtgt tgcccctgca tttgatcaag
     6541 caataacagc atagcttccc gaccgatttc gtaacgcggc tgcgcgatgg ttgtcagcgg
     6601 cggatcacaa aattgcgtca ggtcgatgtt atcaaaaccg attatggaaa ggtcttccgg
     6661 gactttcagc ccctggcgtt ttgcctgaga aagtgcgccg agcgccatca catcgctatg
     6721 gcagaagact gcagtaggcg gttgtggaag atcaagcagc tgttgcattg ctttgcttcc
     6781 ggcttcgaag gtgaagtcgc cacgggcgat gtattgtgga tcaaccataa ttccgcagcg
     6841 acgcagcgcc tgaacatagc cttgcaggcg gtagtgacac agtggcatct cttcgggacc
     6901 ggctatacag ccaatccgtt tatgcccttg ctcatataaa taatttactg catcaaatgc
     6961 ggcggtcaga ttgtcgatat gaacggtagg cagctccagt tccggtgcaa attcgttcgc
     7021 catcaccatc ggcggcagat tacgctgttc ctcaatgctg gcatcaaacg gcagccttga
     7081 acccagcagc aacatgccat caatttgctt ggtgatgatc aaatcgataa aggttttttc
     7141 ctgctgattt tgatgcgcac agtcgccaat cagcaccaga tatccgtgat ttgccgccgt
     7201 aacttcgata ccgcgaataa tttcgctaaa gaagggatcg cagatatccg ggacaatcac
     7261 cagaatggtg cgggattcat tacgcttgac gttgcgcccc ataggctgcg gtaaataacc
     7321 cacttcccgg gccgcttttt caacccgatt acgggtggcc tgggagactt tatcgggatt
     7381 cattaatgct cgggagacag tcgctgtaga gacttttgcc ttgagggcaa cgtctttcat
     7441 ggtcgcggca gtttcctgct tcttcgcttt cacactcact cctcgcctgg cacgtcaggc
     7501 gtactacatc catgtttact tcacatcggc aacattttta gcagatagcg cgtgaaaacg
     7561 gttacagaat tttcatgaaa agtgtgatga atattgaatt tttcgatccg cctcgcatcg
     7621 taagcggttt aaccctcaat cggatcaaca tccagtaccc atttcacctt gcgggaatcc
     7681 ggtattgtat tgatgagcgc cagcgtacca ctaatgatgt gttgcaagcg cacgcgggaa
     7741 gggtgctgca acaatatctg ccagcgccag cggccgccac gtttaggtgc cagtgccgga
     7801 accggaccga gaacccacag tttgtcgtct gccagcggac tggagaggat cagattacgc
     7861 agttgttgca ggaacagcgg cgcatgctga ttgttatgat cttccgcacg cacaatcaca
     7921 tggctggtcc atggcggcag ttgcatcatc cgtcgctcag ccagcgcctg ttcggcaaag
     7981 gcgtcgtagc ctttatagag caacgtttgc aacagaggat gttccggatg gtgcgtttgc
     8041 agcaccactt cgccttgttt acccgcacgc ccggcacgac cagcgacctg ggtgtaaagc
     8101 tgagcgaaac gctctgccga gcgaaaatcg gcagaaaaca gcgcgccgtc cacatccagt
     8161 aacgccacca gcgtcacatc cggaaagtgg tgacctttcg ccagcatttg tgtgccgatc
     8221 aaaatcctcg caccgccgcg atgtacttcc gccagttgct gttccagcgc ccctttgcgg
     8281 ctggtggtat cgcggtcgat acgagaaatg ggcacatcag ggaacagcgg cgcaagcgtc
     8341 tgttcaagct gttcggtgcc cagccccacg ggtaccaggt gcgtggaacc gcaggaaggg
     8401 cactggcgcg gcaccggacg ctgactgtca cagtggtgac agcgcaaatg ctgttgcgcc
     8461 tgatgcagcg tgtagtagtg atcgcaacgt gggcattcgg caatccagcc gcagtcgtgg
     8521 cacagtagcg caggcgcaaa gccacggcgg ttaagaaaga ggataacctg attgttggcc
     8581 tgtaaatgct ggcgcatacg ggtgattaac gccggagcca gacctgcctg caccttctga
     8641 ccttttaaat ccagcacgtg ttgaattgcc ggacgcgcat tccccgcccg gcgcgtcagg
     8701 cgcagcaggc ggtatttttt ctgctggacg ttgcacaacg tttccagcgc gggcgttgcg
     8761 gagccaagaa taatcgggat ttgctcgcta tgcgcacgat acaccgccag gtcgcgggca
     8821 tgatagcgcc agccttcctg ctgcttgtag gaactgtcgt gctcttcgtc aatgacaatc
     8881 acgccgagat ttttaaacgg cgtaaacagc gcggagcggg tgccgatcac aatcgccgcc
     8941 tcgccatttt tcgctttcag ccacgccgaa agacgctcgc tgtcgttcag accggaatgc
     9001 agaacttcca cgggcgcatt aaaccgttca cgaaaacggg cgatagtttg cggtgtcagg
     9061 ccgatttccg gcaccatcac cagcgcctgt ttgccctgag cgagcacgtt ttccagtaca
     9121 ctgagataaa cctccgtttt accggaaccg gtaacgcccg ccagcagcca ggcagaaaaa
     9181 gtatctgccg cgctatgaat tgcgccaacg gcggtggcct gttcggtatt caatcgcaac
     9241 cgctcaccag aaacggcata gttcgttcgc cagtcgctaa actctggtgt ttcacttgct
     9301 aaatcacaca gacctttttt gcgcagcgcc tgcaacgcgg catcattaaa ttcgagcgtg
     9361 gcaacctggt cgcgccagat tttgccttgc cgtaacgctg ccagcgcctg ttgttgcttg
     9421 ggtgagcgtt tcaggctgtt cagatccacc gcctgacctt gttcagtggc aaaccagtac
     9481 cacatcggcg cgttcgccgc aggccgcccc tggcgtagta aaatcggcaa ggcatgaaac
     9541 agcacatcgc caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg
     9601 gagtgagtaa acaccggctc accatccagc acttcgacta ccgcttttag ctcagtgagc
     9661 ggcagttcgc tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca
     9721 aacggcacgc gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca
     9781 aaggtacgag gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga
     9841 cttgaaattc ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata
     9901 cgctggttaa tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata
     9961 cttttaacat cgcgtggtgt ctggcgttag ggctggaaga gcgacgcggc cttaaaccga
    10021 ggttttccca tgaaaaaaga tattcacccg aaatacgaag aaattactgc tagctgctct
    10081 tgcggtaacg taatgaaaat ccgctccacc gttggtcatg acctgaacct cgacgtgtgc
    10141 agcaagtgcc acccgttctt cactggcaaa cagcgtgatg ttgctaccgg tggccgtgtt
    10201 gaccgcttca acaagcgttt caacatcccg ggcagcaaat aagaccggat ttccgaaaaa
    10261 agcgccgcac ggcgcttttt ttgtgcctga attttggtcg tatcaccacg aaaacagcgg
    10321 ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg gtgaaactac
    10381 cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct ggaccagcgg
    10441 gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc ccagcgcgtt
    10501 ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc atgagaagct
    10561 gaaatcatac ggtttaccga gataacgttt tgccgtttgc gtcagttttt gctgttgttc
    10621 aacactcagt ccgccttcaa cgcggcgaac aacgtagttg cccttttcac catgggcaat
    10681 ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt agggcttttt
    10741 gttacgcatc accagcatac cggtgtggct gtaatcggaa tgggttgcca gttggatcgc
    10801 tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg gctgccaggc
    10861 aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca tagttaaacg
    10921 tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc cggatgcggc
    10981 gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa tatgacgtag
    11041 gcctgataag cgtagtgcat caggcgattc cactccgcgc cgctcttttc cgctttagta
    11101 ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg cttccgggat
    11161 ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc cggtaaaggc
    11221 atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt tgttcacctg
    11281 acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg aaaccgtaat
    11341 ctttttgact tgttcactct tcttgccgtg ttcagcgtat gggctgatat attcgccgct
    11401 ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag accccacaat
    11461 ttatgtgggt actgaccgta aacccgcata gtttaccgta caggcgttac cgtgacatcg
    11521 tgtaatgcac ctgtcggcgt gataatgcat ataattttaa cggctatttg ggatttgctc
    11581 aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattaa cacaatgttt
    11641 actctggtgc ctgacatttc accgacaaag cccaggaaac ttcatcacat gacgcgtaaa
    11701 caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg ttgcgttgtc
    11761 cctcccatcc atctttccag cacctataac tttaccggat ttaatgaacc gcgcgcgcat
    11821 gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc agcgtgcgct ggcagaactg
    11881 gaaggcggtg ctggcgcagt acttactaat accggcatgt ccgcgattca cctggtaacg
    11941 accgtctttt tgaaacctgg cgatctgctg gttgcgccgc acgactgcta cggcggtagc
    12001 tatcgcctgt tcgacagtct ggcgaaacgc ggttgctatc gcgtgttgtt tgttgatcaa
    12061 ggcgatgaac aggcattacg ggcagcgctg gcagaaaaac ccaaactggt actggtagaa
    12121 agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca tctggcaagg
    12181 gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt acaaaatccg
    12241 ctggcattgg gtgccgatct ggtgttgcat tcatgtacga aatatctgaa cggtcactct
    12301 gacgtggtgg ctggcgtggt gattgctaaa gacccggacg ttgtcactga acttgcctgg
    12361 tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct gctacgcggg
    12421 ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca acgcgcaggc gattgtgaaa
    12481 tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc ggaaaatcag
    12541 gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg caatgttgag ttttgaactg
    12601 gatggcgatg agcagacgct gtgtcgtttc ctgggcgggc tgtcgttgtt tacgctggcg
    12661 gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac acatgcaggc
    12721 atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg tatctccacc
    12781 ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg ggctgcaaac
    12841 aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag ctgcataaat
    12901 ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg
    12961 agttctctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc actaaccagt
    13021 tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag gttcaacaaa
    13081 cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa gaagccgata
    13141 gcctcattag cgcttttgtc agcgatcttg agcgcctggc ggcgttgctc gacagtggta
    13201 ttaacgatgc ggtgtatgcg gaagtggtgg gccacggtga agtgtggtcg gcgcgcctga
    13261 tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc cgtgagtttt
    13321 tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg gctttcttac ccgttgctgc
    13381 aacagctact ggtacaacat ccgagcaaac gtctggtggt gaccgggttt atcagccgta
    13441 acaacgccgg tgaaacggtg ttgctggggc gtaacggttc cgactattcc gcgacacaaa
    13501 tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca
    13561 gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt ctggatgagg
    13621 ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc ccgtacttta cagccagttt
    13681 ctggcagcga aatcgacctg cagctgcgct gtagctacac gccggatcaa ggttccacgc
    13741 gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc cacgatgatg
    13801 tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg cataaagaga
    13861 tcgaccagat cctgaaacgt gcccaggtgc gtccactggc ggttggcgtg cataacgatc
    13921 gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg aaaatcctcg
    13981 acgaagcggg attacctgga gaactgcgcc tgcgtcaggg gctggcgctg gtggcgatgg
    14041 tcggggcggg tgtcacccgt aacccgctgc attgccaccg cttctggcag caactgaaag
    14101 gccagccggt cgagtttacg tggcagtccg atgacggcat cagtctggtg gcagtactgc
    14161 gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc cgcgcagaaa
    14221 aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg ctctcgctgg ctggaactgt
    14281 tcgcccgcga gcagagcacg ctttcggcgc gtactggctt tgaatttgtg ctggcaggcg
    14341 tggtggacag ccgccgcagt ctgttgagtt atgacgggct ggacgccagc cgcgcgttag
    14401 ccttcttcaa cgatgaagcg gtcgagcagg atgaagagtc gcttttcctg tggatgcgcg
    14461 cccatccgta tgatgattta gtggtgttgg acgttaccgc cagccagcag cttgctgatc
    14521 agtatctcga tttcgccagc cacggtttcc acgtcatcag tgccaacaaa ctggcgggag
    14581 caagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact
    14641 ggctatacaa cgccaccgtt ggtgcgggct tgccgatcaa ccataccgtg cgcgatctga
    14701 tcgacagcgg cgataccatt ttgtcgatca gtgggatctt ctccggcacg ctctcctggc
    14761 tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc
    14821 agggcttaac cgagcctgat ccgcgtgacg acctctccgg caaagacgta atgcgcaagc
    14881 tggtgattct ggcgcgtgaa gcgggttaca acattgaacc ggaccaggta cgtgtggaat
    14941 cgctggtgcc tgctcattgc gaaggtggca gcatcgacca tttctttgaa aatggcgatg
    15001 aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg ctggtgctgc
    15061 gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag gcggtgcgtg
    15121 aagaccatcc gctggcatca ctgctgccat gcgataacgt ctttgccatc gaaagccgct
    15181 ggtaccgcga taatccgctg gtgatccgtg ggcctggcgc tgggcgcgac gtcaccgccg
    15241 gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag aaatttaata
    15301 atgcccggta ctcatgtttt cgggtctatg gtttctaatg aaatatattg aattatcata
    15361 gaattaggcc ggatgaagcg tttacgacga atccggcaag aagcaataag tacatggtta
    15421 gtttatattt gcagtccggt ttgctttgca tatcggattt tctttttctt accatcctga
    15481 agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc
    15541 ttcggctttt ccttcatctt tacatctgga cgtctaaatg gatagatgtg cacaacacaa
    15601 catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
    15661 atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt
    15721 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
    15781 gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta
    15841 cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta
    15901 cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac tggaataacg
    15961 gtatttgtca tatcgtggca ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa
    16021 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atatccgtgg
    16081 cggcgtatcc ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga
    16141 aacgcaaagt ggatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa
    16201 gctacctgcg ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa atcatcccgg
    16261 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
    16321 gtattccggc gtggatggcg caaatgtttg acgggctgga tgacgatgcc gaaacccgca
    16381 aactggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
    16441 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc
    16501 tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca
    16561 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag
    16621 ccttgttttc tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat
    16681 ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat ctacatctct
    16741 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
    16801 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
    16861 tcagacgata tccataacac cacagccact ggcaaatgcc cgttccatca gggcggtcac
    16921 gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt
    16981 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
    17041 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
    17101 gaatctcagc cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
    17161 gcttggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt
    17221 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
    17281 cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt
    17341 atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
    17401 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
    17461 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
    17521 ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca
    17581 gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt
    17641 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
    17701 gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
    17761 ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca gacgccgacc
    17821 cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca gacccgcagt
    17881 ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccgttc
    17941 gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct gcgttttgat
    18001 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
    18061 tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc
    18121 gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
    18181 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
    18241 gttagtgagc tggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa
    18301 cgcggtggtg tcaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac
    18361 gccgcagccg ttcgtgctct gcttgttctg gagaaaatcc agaaagagtc tggtaaagcc
    18421 tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc
    18481 gcaggtttga gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag
    18541 actgacattg agatgtttga gctgctggag ccaattgctg acggtttccg taactatcgc
    18601 gctcgtctgg acgtttccac caccgagtca ctgctgatcg acaaagcaca gcaactgacg
    18661 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg tgccaacttc
    18721 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
    18781 gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa agagctgttc
    18841 gaaggacgtg accgcgaaac cggcgaagtg aaatacacag ccagccgtgc ggatctggta
    18901 tttggttcta actctgtcct gcgtgcagta gcggaagttt acgccagcag cgatgcccac
    18961 gagaagtttg ttaaagactt cgtagcggca tgggtgaaag tgatgaacct cgaccgtttc
    19021 gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct gaagtttctt
    19081 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
    19141 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
    19201 tggattttca tgaagcaagt caccagttac atcggtgcct tcgactttac cgccttacgc
    19261 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
    19321 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggt
    19381 ctggcgcagt gggcgttggt cagcggaggt gcggggaagg tggcgatcct gagctatacc
    19441 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
    19501 caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
    19561 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt tagttggggg
    19621 gcgagcgcga ttgttgctaa acgcctatac gcccgtcatc cgcgcgtgga tttattatcg
    19681 ttaacatcct ggcagatgct gtatgcggcg ctggtgatga gtgtggtcgc tttactggtg
    19741 ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt
    19801 ctggcgacgg cgctagcgtg gagcttatgg ctgtttgtgc tgaaaaactt gcctgccagt
    19861 attgccagct taagcacact ggccgttccc gtttgtggcg tactcttttc ctggtggctg
    19921 ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt gctggcactg
    19981 gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg aattttttct
    20041 tcatgtgggg cgatctctta tttaacaaaa taacgataat gccccaccat ccgccagcta
    20101 aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg
    20161 tcgcgggcga agccatctga cgccacccca atatgcgcca gcccgttatc taatcgccag
    20221 gagacaatat cgcccgcttg atagtcaccg gcatttttac tgatggggcg tgttttgttg
    20281 tggcggctaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
    20341 cgctttaact tccatttttg cgggtactca gcaaaattct tcgccatatc ttcgtgaacc
    20401 agtttctgca aatcgacctt ctggctgcgc aatgcgcgga tcaccacatc ggaacataca
    20461 ccgcgttctt gcggaacatc accgccagga taggtaagct gcatatacgc cggatcgtaa
    20521 aatagcgtgc tgccaatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc
    20581 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc
    20641 gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt
    20701 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
    20761 aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggagt
    20821 gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag attgcgtagg
    20881 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    20941 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcag ctaccagcag
    21001 tgcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    21061 tgcacaagat gcttttgcaa caatgcgcat tttcgccggg acatcttctt taatatccag
    21121 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
    21181 ctccaccggc gcgttttcca gtaccagctg cgtcagcgta ccaaatgcca ctttttcacc
    21241 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg cgtgagccgc
    21301 cgctttcaaa accaacaccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
    21361 gggttactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc agcagggtgt
    21421 tgtagcacag ttcagccagt gccagcgccg cctgggtgca cttgccgccc gccatggtgg
    21481 tcgcgccgct acgagagcaa gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
    21541 ccgctgctaa cagacgtgca ggtgcgcccg cgacgatttt ggtgtcgaca atgaccatat
    21601 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
    21661 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
    21721 ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg ataccgagaa
    21781 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
    21841 attcaccgcc aaacggcgta atttctacta ccagtccagc atctttaaag cttttctcga
    21901 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
    21961 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
    22021 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
    22081 ttccagtcgt gttcgaactt ctctatagct gactctactg caggggtgtt gagcatttgt
    22141 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
    22201 tgacacggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
    22261 agcgtttgca gctcctgaac cgtacgaatg ccgtccccgc cctgggcatc tacgcggtta
    22321 acatacggag caacgtattt tgcccctgcc agtgcggcta ataacccttc tgcggcgcta
    22381 tatacagcgg tgccaagtgt agtaataccc tcttttttca gtattttaat tgctgccaga
    22441 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
    22501 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
    22561 tcaccaatcg ctttttgcag acgcggcagc acttcccaga tggactcctt gctggcagcg
    22621 ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag acgttcgact
    22681 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
    22741 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
    22801 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
    22861 cagcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg ccccagcgca
    22921 ccggcgcttg cagacgcgcc acgctgatgg tggattgctc aatgtgttct gatttgctgt
    22981 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccataagt
    23041 cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta tcggtcatgc
    23101 ctttgagcac ttcttctttg ctgcgccagt cactttccag cgtgatgcac tcggcggtga
    23161 ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc acttccagcg
    23221 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
    23281 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
    23341 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
    23401 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
    23461 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
    23521 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
    23581 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
    23641 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
    23701 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
    23761 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
    23821 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
    23881 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
    23941 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
    24001 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
    24061 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
    24121 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
    24181 aacgagctac ggcttctcct ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
    24241 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
    24301 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccgcttttc agtaacaatc
    24361 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
    24421 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
    24481 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tatctgctgc
    24541 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
    24601 ttaatccggc gctgactcct gccagtaaat gttcgtgcag ggaagtatcg ccagccaggg
    24661 atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
    24721 tgtttttcag taccagcgtc aggccgtttt ccagtgcgga ttgctcggcg tcaacgtctt
    24781 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
    24841 cgcctgcact accgctgcac acggtgcgga cgcggataat ttgcggattc agattggtca
    24901 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
    24961 gggggaattc atcgcgcagc cattggctta aacgctggtg cccttcctgt tcgtcggccc
    25021 cggaaatcag tagctggcag ttatcaccca ccagcgtatc ggtgccaatc agcgccagag
    25081 cgcttttggc gttgccttta cggtcagtac gcaggttatg ccactcaatt tgagatgaaa
    25141 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
    25201 cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt tctgtttaca
    25261 ggataagagc gcacggtaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
    25321 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    25381 attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    25441 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    25501 tatggacatg ggcttttgca agcccgggag cagcttatga atgagttggt gcagatcctg
    25561 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    25621 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    25681 gtagccgatc cgaatttgaa aaaactgttt gatattggcg ttgcaggttt gacgctgatg
    25741 gtgcctttcc tcgccgctta cattggttac tccattgcag agcgttctgc gctggctccg
    25801 tgcgctatcg gtgcctgggt tggtaacagc tttggtgcgg gcttctttgg tgcgctgatc
    25861 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    25921 ctgcgctcgg tgatgcccat cttcattatt cctatcatcg gcacactgat taccgcaggc
    25981 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    26041 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg gctgatgctg
    26101 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    26161 gctcagggtg tttataccgt ggtggccatt gctgccgtgg gtatttgtat cccgccgctg
    26221 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    26281 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc
    26341 gctgccgatc cgctgcgtgt cattccttcc attatggtcg gttcagtttg tggtgcagta
    26401 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    26461 gtggttgaag gtaagttggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    26521 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    26581 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    26641 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggta gcagaggcgc
    26701 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag gggtcaattg
    26761 gtctggaaaa tgaactgact gcggaagacg tggcgagcgc tgatatggtg attctgacca
    26821 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    26881 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    26941 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttcccac aggagttcct
    27001 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    27061 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    27121 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    27181 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    27241 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    27301 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    27361 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    27421 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    27481 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    27541 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    27601 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    27661 ccagcgtcgc gaagagctgc tgactattgc ggagatctcc cgccataacg cgcaacataa
    27721 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    27781 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    27841 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    27901 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc gttatttcgc
    27961 cggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    28021 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    28081 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    28141 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtgccagc
    28201 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    28261 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    28321 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    28381 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    28441 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    28501 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    28561 cggcgtacag ggaattggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    28621 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    28681 tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg agaaatacgg
    28741 taacgatatc gacgaggtgg ataacattag cgccgaactg ttgcgccact actgcaaaga
    28801 agtggaaaaa taccagaacc cgcgcggcgg ttacttcacg ccgggatcgt ataccgtttc
    28861 tgctcacgtt ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    28921 acaactggca gacggcggct tgtcacctat gctgggtcag gacgcacaag ggccaacggc
    28981 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    29041 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    29101 cttacgggtg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    29161 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    29221 tggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    29281 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    29341 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    29401 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    29461 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    29521 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    29581 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    29641 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    29701 gtgtctcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    29761 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    29821 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    29881 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc cgggagaata
    29941 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    30001 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    30061 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    30121 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    30181 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    30241 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    30301 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    30361 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    30421 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    30481 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagt
    30541 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    30601 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    30661 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    30721 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    30781 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    30841 ggatttggct gccgcgcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    30901 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    30961 ctatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    31021 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    31081 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    31141 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    31201 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    31261 tctggcgcag ggacagcggg ccgttgatca ggcgggagag caaatagctg acgtcgtgat
    31321 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    31381 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt atcgcatccg
    31441 gcaaatagcg ccagacgcta tacaaaggtc atctactgat tacccacctg atcgccatac
    31501 ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    31561 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    31621 cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg atcgacatcc
    31681 tgtgagaaat cacggggatg agtcgcttgc catttttccg acgtccacag caggaacgga
    31741 atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc cctgagtttt gtgcggcggc
    31801 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    31861 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    31921 taatcgttat atgattccag ctcttctgcg cttaatcctg gaggaacatg atcggtattg
    31981 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    32041 agatgaacga tgatcagttt cttcggcgca gggtcattca gcacatcctg gaacggcttc
    32101 agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    32161 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    32221 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    32281 gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    32341 atggtgtacg gacgagacgt aactacgtta ttaaacacgg tcagattcgg atcggtttta
    32401 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtccg
    32461 cgctgggtcg actcgccaat caccagcact aaagtgcgcg gttcgttacc cgattcatct
    32521 ttgaaattag ccagtggcgg caaggcatta ttttcattca gtaactttgt tagcgagttt
    32581 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    32641 cgcgaggcta ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    32701 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaacata acgccacggc
    32761 tttggaatat agaccgggcg caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc
    32821 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    32881 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    32941 tagtagcaca gcgccgccag agaggccgtc catagcacca cgccgatcac tgcggcaata
    33001 attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgagct gaataacagc
    33061 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    33121 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct agaaaggggt
    33181 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    33241 tgtaacgaca attccttaag gatatctgaa ggtatattca gaatttgaat aaaatgcaga
    33301 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    33361 aagcacgagg gtttgcagaa gaggaagatt agccggtatt acgcatacct gccgcaatcc
    33421 cggcaatagt gaccattaac gcttgttcga cgcgaggatc cggttcctgg ccttcttttt
    33481 ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg tcggtgtaaa
    33541 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    33601 tggcaatcgc cagcaccact ttgatgtctt cttcttgcag gttgcgtaac tctttaccta
    33661 acggccacag tgctttgtct accagacgtt gatcatagta ttccgccaac cacaggtctg
    33721 ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    33781 acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gccgtacctg
    33841 cacccagcca ggcggggagc atcaggcggt tttgcgtcca ggcaaaaatc cacggtatgg
    33901 cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc cggacgtgaa cccaacggca
    33961 gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt
    34021 cacgtacgta gccacggtag agatcgcagg agatgactga cagttcatcc ataatgcgac
    34081 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    34141 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    34201 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    34261 catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    34321 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    34381 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    34441 tgccctgaat caggccgcga taccagtcga tattgagcag ctgggtcatg acatcgttgg
    34501 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca aacccgatac
    34561 ccgcttcttt cagcagcagg tggacagcca gtacgtcgga cggcgttttc gccatcgaga
    34621 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    34681 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    34741 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    34801 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    34861 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    34921 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    34981 cccacagttc ttcgttttgt gtcagcagcc cttctggttt tggcagttct tcgcctttca
    35041 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    35101 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    35161 caaccatcga cagttcagaa accagaacct gaatatcttt caggaacaga tcggtggctt
    35221 tccagcggct gagcagcagg acgtggcggg tgatatcggc agtgacgttc gggttgccgt
    35281 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    35341 agtcgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    35401 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    35461 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggtgcagg cgacgcatca
    35521 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    35581 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    35641 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    35701 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    35761 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    35821 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    35881 ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata gtttctacgc
    35941 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    36001 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    36061 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    36121 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    36181 ttcagcaaac gaataaatag caggaattta cgccattaaa ttcacgacgc tttaaataag
    36241 cgtaacttat ggaaatgtta aaaaatcgcc atacataaca ccaaaggtgt aggtcggata
    36301 agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg agtttggcaa cggtgccgga
    36361 tgcggcgcga gcgccttatc cggcctacgt tttaatgcca gcaaaaatgg tgaattacct
    36421 ggataatcaa ctcacgggtg ggtttaataa accgcgtctc cagatattca tcaggctgat
    36481 gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg gcataacgtt tgaataaacg
    36541 gcgcttcggt acagtaattc accacttcgg tttttgctcc gagcaatttc tcaaccactt
    36601 caaccagttg atgattcggt gggcattcat agccagggat cggcggatgc agctcgtcga
    36661 ccgtcagacg acccggccag cgttcgctca ccggagccaa tgcatcgttg agcaaaccat
    36721 taagttcatt gagtgtcatg ccaggctgcg gacgaatatc catatgcaac tcacagcaag
    36781 cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc gaggttgagc gtagggtatg
    36841 gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat
    36901 gcccgatggc gtcgtgcatt agttcgatag cgttaactcc gcgtgctgga tcgctggagt
    36961 gccccgactg gccctgaata cggatggcgt tagagatatg acctttatgt gcgcgtaccg
    37021 gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg
    37081 caaaataacg cgctccggcc atactggttt cttcatcagc agtcgccaga atgtagagcg
    37141 gttttttcag tttcgtgacg tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc
    37201 ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg
    37261 gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca
    37321 agccgccagc cccctgtccg taactggcca gcatattaaa tttgttgcga gttcctggaa
    37381 caggctgcac ttccacattg aagcccaaat ctttaaacca gtccgccagc agagtgatta
    37441 aatctgcatt gctttgatcg agtgcctctt ccgtagcgct tattgaaggt gtggcaatca
    37501 gagcgcggta aatctcgata aatggcggta atttgttttt cattgttgac acacctctgg
    37561 tcatgatagt atcaatattc atgcagtatt tatgaataaa aatacactaa cgttgagcgt
    37621 aataaaaccc accagccgta aggtgaatgt tttacgttta acctggcaac cagacataag
    37681 aaggtgaata gccccgatgt tgaatacgct gattgtgggt gccagcggct acgctggcgc
    37741 agagctagtg acctatgtaa atcgccatcc gcatatgaac ataaccgctt tgactgtttc
    37801 agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg catccgcagc taaaaggcat
    37861 cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag tttagcccag gggtggacgt
    37921 agtgtttctc gccaccgccc atgaagttag ccacgattta gcgccgcagt ttcttgaagc
    37981 gggctgcgtg gtgttcgacc tttccggcgc gtttcgtgtt aacgacgcca ccttctatga
    38041 aaaatattac ggctttaccc atcaataccc ggaactgctg gaacaggcag cctacggtct
    38101 ggcggagtgg tgcggtaata aattaaaaga agcgaatttg attgcggtgc caggctgtta
    38161 tccgacggcg gcacagctgg cgctgaaacc gttgattgat gccgatcttc ttgacctcaa
    38221 tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat
    38281 ttcaaatagt ttttgtgaag ttagcctgca accgtatggc gtctttactc atcgccatca
    38341 accagagatc gccacacacc tcggtgctga cgttatcttc accccacatc tgggcaattt
    38401 cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa tcgggtgtga cccaggcgca
    38461 agtcgcgcaa gcgttacagc aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa
    38521 aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt tgcgatatcg ggtttgccgt
    38581 tcagggcgag catctgatta ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc
    38641 acaagcggta cagtgcgcca atattcgttt cggctatgcg gaaacgcagt ctcttattta
    38701 agggtgcaat gatgaatcca ttaattatca aactgggcgg cgtactgctg gatagtgaag
    38761 aggcgctgga acgtctgttt agcgcattgg tgaattatcg tgagtcacat cagcgtccgc
    38821 tggtgattgt gcacggcggc ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc
    38881 cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga tcagatagac attatcaccg
    38941 gagcactggc gggaacggca aataaaaccc tgttggcatg ggcgaagaaa catcagattg
    39001 cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa agtgacccag cttgatgaag
    39061 agttaggtca tgttggactg gcgcagccag gttcgcctaa gcttatcaac tccttgctgg
    39121 agaacggtta tctgccggtg gtcagctcca ttggcgtaac agacgaaggg caactgatga
    39181 acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac gctgggcgcg gatctgattt
    39241 tgctctccga cgtcagcggc attctcgacg gcaaagggca acgcattgcc gaaatgaccg
    39301 ccgcgaaagc agaacaactg attgagcagg gcattattac tgacggcatg atagtgaaag
    39361 tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc ggtagatatc gcttcttggc
    39421 gttatgcgga gcagcttccg gcactgttta acggtatgcc gatgggtacg cggattttag
    39481 cttaagtttt gttagccgga ggagcagctt tccggcattg aatttcaaaa taaggaaaca
    39541 gagttatggc actttggggc gggcgtttta cccaggcagc agatcaacgg ttcaaacaat
    39601 tcaacgactc actgcgcttt gattaccgtc tggcggagca ggatattgtt ggctctgtgg
    39661 cctggtccaa agccttggta acggtcggcg tgttaaccgc agaagagcag gcgcaactgg
    39721 aagaggcgct gaacgtgttg ctggaagatg ttcgcgccag gccacaacaa atccttgaaa
    39781 gcgacgccga agatatccat agctgggtgg aaggcaaact gatcgacaaa gtgggccagt
    39841 taggcaaaaa gctgcatacc gggcgtagcc gtaatgatca ggtagcgact gacctgaaac
    39901 tgtggtgcaa agataccgtt agcgagttac tgacggctaa ccggcagctg caatcggcgc
    39961 tggtggaaac cgcacaaaac aatcaggacg cggtaatgcc aggttacact cacctgcaac
    40021 gcgcccagcc ggtgacgttc gcgcactggt gcctggccta tgttgagatg ctggcgcgtg
    40081 atgaaagccg tttgcaggat gcgcttaagc gtctggatgt cagcccatta ggctgtggcg
    40141 cgctggcggg aacggcctat gaaatcgacc gtgaacagtt agcaggctgg ctgggctttg
    40201 cttcggcgac ccgtaacagt ctggacagtg tttctgaccg tgaccatgtg ttggaactgc
    40261 tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt tgctgaagat ctgattttct
    40321 ttaacaccgg cgaagcgggg tttgtggagc tttcggaccg cgtgacttcc ggttcatcat
    40381 taatgccgca gaagaaaaac ccggatgcgc tggagctgat tcgcggtaaa tgcggccggg
    40441 tgcagggtgc gttaaccggc atgatgatga cgctgaaagg tttgccgctg gcttacaaca
    40501 aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct cgatacctgg ctggactgcc
    40561 tgcatatggc ggcgctggta ctggacggca ttcaggtgaa acgtccgcgt tgtcaggaag
    40621 cggcgcagca ggggtatgcg aacgccactg aactggcgga ttacctggtg gcgaaaggcg
    40681 tacctttccg cgaggcgcac catattgtgg gtgaagcggt ggtggaagcc attcgtcagg
    40741 gcaaaccgct ggaagatctg ccactcagtg agttgcagaa attcagtcag gtgattggcg
    40801 aagatgtcta tccgattctg tcgctacaat cgtgcctcga aaagcgtgcg gcaaaaggcg
    40861 gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc gcaggctcgg ttagagtaat
    40921 ttgtttatat tgctcccctg aaaatgggga gtttaccacg cctacaaagt gcgacacttc
    40981 gtaggcggat aaggcgttta cgccgcatca ggcagaaatt tgtcagcatt c
//
LOCUS       DADLTI010000013        40824 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLTI010000013 DADLTI010000000
VERSION     DADLTI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.013"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(209..1000)
                     /gene="cpoB"
                     /locus_tag="JN755_000680"
     CDS             complement(209..1000)
                     /gene="cpoB"
                     /locus_tag="JN755_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7379578.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1010..1531)
                     /gene="pal"
                     /locus_tag="JN755_000681"
     CDS             complement(1010..1531)
                     /gene="pal"
                     /locus_tag="JN755_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7379579.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1566..2858)
                     /gene="tolB"
                     /locus_tag="JN755_000682"
     CDS             complement(1566..2858)
                     /gene="tolB"
                     /locus_tag="JN755_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7379580.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2991..4247)
                     /gene="tolA"
                     /locus_tag="JN755_000683"
     CDS             complement(2991..4247)
                     /gene="tolA"
                     /locus_tag="JN755_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7379581.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4312..4740)
                     /gene="tolR"
                     /locus_tag="JN755_000684"
     CDS             complement(4312..4740)
                     /gene="tolR"
                     /locus_tag="JN755_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7379582.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4744..5436)
                     /gene="tolQ"
                     /locus_tag="JN755_000685"
     CDS             complement(4744..5436)
                     /gene="tolQ"
                     /locus_tag="JN755_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7379583.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5433..5837)
                     /gene="ybgC"
                     /locus_tag="JN755_000686"
     CDS             complement(5433..5837)
                     /gene="ybgC"
                     /locus_tag="JN755_000686"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7379584.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5987..6280)
                     /gene="ybgE"
                     /locus_tag="JN755_000687"
     CDS             complement(5987..6280)
                     /gene="ybgE"
                     /locus_tag="JN755_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7379585.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6280..6393)
                     /gene="cydX"
                     /locus_tag="JN755_000688"
     CDS             complement(6280..6393)
                     /gene="cydX"
                     /locus_tag="JN755_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7379586.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6408..7547)
                     /gene="cydB"
                     /locus_tag="JN755_000689"
     CDS             complement(6408..7547)
                     /gene="cydB"
                     /locus_tag="JN755_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7379587.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7563..9131)
                     /gene="cydA"
                     /locus_tag="JN755_000690"
     CDS             complement(7563..9131)
                     /gene="cydA"
                     /locus_tag="JN755_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7379588.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9980..12613)
                     /gene="mngB"
                     /locus_tag="JN755_000691"
     CDS             complement(9980..12613)
                     /gene="mngB"
                     /locus_tag="JN755_000691"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7379589.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12631..14607)
                     /gene="mngA"
                     /locus_tag="JN755_000692"
     CDS             complement(12631..14607)
                     /gene="mngA"
                     /locus_tag="JN755_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY7379590.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAIDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            14717..15439
                     /gene="mngR"
                     /locus_tag="JN755_000693"
     CDS             14717..15439
                     /gene="mngR"
                     /locus_tag="JN755_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7379591.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15543..16412)
                     /gene="sucD"
                     /locus_tag="JN755_000694"
     CDS             complement(15543..16412)
                     /gene="sucD"
                     /locus_tag="JN755_000694"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7379592.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16412..17578)
                     /gene="sucC"
                     /locus_tag="JN755_000695"
     CDS             complement(16412..17578)
                     /gene="sucC"
                     /locus_tag="JN755_000695"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7379593.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17672..18889)
                     /gene="odhB"
                     /locus_tag="JN755_000696"
     CDS             complement(17672..18889)
                     /gene="odhB"
                     /locus_tag="JN755_000696"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7379594.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18904..21705)
                     /gene="sucA"
                     /locus_tag="JN755_000697"
     CDS             complement(18904..21705)
                     /gene="sucA"
                     /locus_tag="JN755_000697"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7379595.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22006..22722)
                     /locus_tag="JN755_000698"
     CDS             complement(22006..22722)
                     /locus_tag="JN755_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7379596.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22738..24504)
                     /gene="sdhA"
                     /locus_tag="JN755_000699"
     CDS             complement(22738..24504)
                     /gene="sdhA"
                     /locus_tag="JN755_000699"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7379597.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24504..24851)
                     /gene="sdhD"
                     /locus_tag="JN755_000700"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24504..24851)
                     /gene="sdhD"
                     /locus_tag="JN755_000700"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7379598.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24845..25249)
                     /gene="sdhC"
                     /locus_tag="JN755_000701"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24845..25249)
                     /gene="sdhC"
                     /locus_tag="JN755_000701"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7379599.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25630..25737
                     /locus_tag="JN755_000702"
     CDS             25630..25737
                     /locus_tag="JN755_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379600.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25943..27226
                     /gene="gltA"
                     /locus_tag="JN755_000703"
     CDS             25943..27226
                     /gene="gltA"
                     /locus_tag="JN755_000703"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7379601.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27617..28183
                     /locus_tag="JN755_000704"
     CDS             27617..28183
                     /locus_tag="JN755_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7379602.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28234..30693
                     /locus_tag="JN755_000705"
     CDS             28234..30693
                     /locus_tag="JN755_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7379603.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30708..31439
                     /locus_tag="JN755_000706"
     CDS             30708..31439
                     /locus_tag="JN755_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7379604.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31436..32498
                     /locus_tag="JN755_000707"
                     /pseudo
     CDS             31436..32498
                     /locus_tag="JN755_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32605..33696
                     /locus_tag="JN755_000708"
     CDS             32605..33696
                     /locus_tag="JN755_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7379605.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33693..34484)
                     /gene="nei"
                     /locus_tag="JN755_000709"
     CDS             complement(33693..34484)
                     /gene="nei"
                     /locus_tag="JN755_000709"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7379606.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34520..35254)
                     /gene="pxpA"
                     /locus_tag="JN755_000710"
     CDS             complement(34520..35254)
                     /gene="pxpA"
                     /locus_tag="JN755_000710"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7379607.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35244..36176)
                     /gene="pxpC"
                     /locus_tag="JN755_000711"
     CDS             complement(35244..36176)
                     /gene="pxpC"
                     /locus_tag="JN755_000711"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7379608.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36170..36826)
                     /gene="pxpB"
                     /locus_tag="JN755_000712"
     CDS             complement(36170..36826)
                     /gene="pxpB"
                     /locus_tag="JN755_000712"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7379609.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36849..37592)
                     /gene="ybgI"
                     /locus_tag="JN755_000713"
     CDS             complement(36849..37592)
                     /gene="ybgI"
                     /locus_tag="JN755_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7379610.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37863..39344
                     /gene="dtpD"
                     /locus_tag="JN755_000714"
     CDS             37863..39344
                     /gene="dtpD"
                     /locus_tag="JN755_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7379611.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39494..>40824)
                     /gene="phrB"
                     /locus_tag="JN755_000715"
     CDS             complement(39494..>40824)
                     /gene="phrB"
                     /locus_tag="JN755_000715"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7379612.1"
                     /translation="VLALYIATPRQWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARVQTLAAYETARKGK"
BASE COUNT         9867 a        11046 c         9888 g        10023 t
ORIGIN      
        1 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccactttt tcgttgcttt
       61 cggtttgttt gatatcccgt ggcaacggcg gcatatatta ctgatttcag acttgagcgc
      121 aacaaaaatt tcgatgcaga tcactcaact gcttatgatt cgcacgacac gaccagaaat
      181 aatgcgactt ctggtcgtgt tttatgcatt acatcgcgtt cagacgtttt tgtgcctgtt
      241 tagcgccatc agtaccaggg tatttactga taacctgctg gtatacggct ttcgcttttg
      301 cggtgtcacc tttgtcctgc atgatgacgc cgactttaaa catcgcatca gcagcctttg
      361 gtgacttcgg atagtttttc actaccgaag caaaatagta cgccgcatca tcttttttac
      421 ccttgttgta gtttaactga cccagccaat aattggcgtt tggcaggtaa gttgaatcag
      481 ggtaattttt gatgaaattc tgaaatgcca ccattgcgtc atcctggcgg gatttatcct
      541 gcaccagcgc aatagctgca ttgtaatccg tgtttgcatc gccgcttttc accggcgcgc
      601 cagcattcgc agtaccagca tcagctgtcg gcgtcgttga tgccgccgca ccgctttgat
      661 cgctgctggt tgattgcgcc gctgcaccac cgctgctgag gctgtcgatc tgcaacagga
      721 tctgcttttg ccgctccacg acctgattca gttgatactg attttcctga atctgaccac
      781 gcagggaatc aatatcggat tgattatcag aaagttgttg ctggagttgg gttaaaagct
      841 ggctgtgagc attagaaata cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc
      901 cgacactact gattggtgcc tgagcaaaag cggcccaggg ggccgctata ccaaccagta
      961 acgacagact caatagttga tgtctgaagt tactgctcat gcaattctct tagtaaacca
     1021 gtaccgcacg acggtttttg gagtatgctg cttcgtcatg acccagtact gcaggttttt
     1081 ctttaccgta agaaacgatg gagatctggt ctgcagaaac gcctttaccc tgcaggtaca
     1141 tcttaacggc gttcgcacga cgttcaccca gggagatgtt gtattccgga gtaccacgtt
     1201 cgtccgcgtg accttctacg gtgactttgt aagacgggtt gctacgcagg aagtttgcat
     1261 gtgcatccag catttgagcg aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa
     1321 cgatgttgtt ctgctgcagc tgttgcattt gcagacgagc ctgctcttcg gaagacatgt
     1381 tgccgttgcc gccgttcgca tccataccag tgccagcacc cagcatgcct tcgctgccgt
     1441 cattgctggc gttcttgttg gaagaacatg ccgcaattgc cataacaggc agagcaatca
     1501 tcagcccttt cagcactttg ttcagttgca tttcaatgat tcctttacta ttcaattaat
     1561 tattatcaca gatacggcga ccaggcaggg aatttgacct gtccatcagt tgccggaaga
     1621 cgcgctttga aacgcccatc tgtagaaacc aaattcagca cggatcccat cccctgagaa
     1681 gagctgtaga ttaccatagt gccgttaggt gccagacttg gcgtttcatc caggaacgtg
     1741 gacgacagaa cttgtacgcc tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca
     1801 ccattggagc tgaccattac cataaattta ccgtcgctgc tgacatccgc atcctggttc
     1861 tgcgaacctt cccaggtaat acgttgtggc gcaccgccgt tgatattcac tttataaacc
     1921 tgcggacgac cggcctggtc agaagtaaat gccaggttct ggctatccgg gaaccaggtc
     1981 ggttcggtat tgttactgcg accatcagtc acctggcgga tctgaccaga agccaaatcc
     2041 attacgtaca ggttcagact accggttttc gacaaggcga atgccagttt gctgccgtct
     2101 ggcgagaatg caggtgcacc gttgtgacgc gggaatgaag ccacctgacg tacagcgcca
     2161 tttgccagcg tctgaataac cagcgcggaa cgaccgcttt cgaaggtcac ataagccagt
     2221 ttagaaccgt ctggtgacca cgccggagac atcagcggct gcggtgaacg gtgaacgaca
     2281 aactggttgt aaccgtcata gtcagatacg cgcagttcat acgggaactg accgccgttg
     2341 gtctgaacaa cgtaggcaat acgggtacgg aacgcacctt taatgccggt cagtttttca
     2401 aacacttcat cactggcggt atgaccagca taacgcagcc actgcttgtt cactttgtac
     2461 gagttctgag caagtacagt acccggtgcg ccgccagtgt caacaagttg ataagcaaca
     2521 ttgtaagaac catccggatt cggagtgacc tgaccgacaa ctacagcgtc aatgcccagt
     2581 gcggaccatg cagctggttg tacttcctgc gcactacccg gctgctgtgg cagacgagcg
     2641 cgatctaacg gattaaattt accgctgtta cgcaagtcag cagcaacgat gccgccaata
     2701 tcttcaggtg ccgcaccagg ccccgcccac tggaaaggaa caacaccaat aggacgaccg
     2761 gaatctacac cgctgtcgat cacaatgcgg acttcagcat gcagaactga tgcccacagt
     2821 atgagaaaac caaatgctac tcgtaatgcc tgcttcatca tatctccctt atctggaccg
     2881 atggcccacg ataatttagc agaatgttaa caaactaaaa tacacaaaac taccagaacc
     2941 ccgtggcaac cggtgcctga tgttgaccgt ccgaacagtc aacatcgcga ttacggtttg
     3001 aagtccaatg gcgcgttttt gaacacttca tatactgcct ggcttggtgg tttcgggatc
     3061 ttcgcaagtt tagctgctgc caacgcagcc tgacaaagtg cgggatcgcc accttcaggt
     3121 ttgatatcca gtaacatacc atcgggtgcc agttttatgc gcagcgtaca ggttttgcct
     3181 gcataggacg atgcgtcata gaacttactt tcgatagcag atttaatctg cccggcatag
     3241 ttattgatat cggcccctga tgcgccattg tttttagtat taccactccc ggcaggcgaa
     3301 gcattgttcc ctttcgcccc tccccccgtt ttcggtgcat tcttaccaga gcttagctca
     3361 ccgaaaatat catctgcctc tgcggcagct tttgctgcag cggctttttc agctgctgct
     3421 tttgccgctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct
     3481 gctgccttat cagcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct
     3541 gccgctttct tatcagctgc agccttatca gctgcagcct tttcagcagc cgctttcttc
     3601 tcagcttctg ccttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct
     3661 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctac ctcggctttt
     3721 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3781 gccgctttct tcgcagcttc ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc
     3841 acttcagctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
     3901 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
     3961 tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa
     4021 cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa
     4081 tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc
     4141 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
     4201 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
     4261 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
     4321 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
     4381 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
     4441 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc
     4501 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
     4561 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
     4621 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa
     4681 cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
     4741 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
     4801 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgagacggtt
     4861 ataagccatt accgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc
     4921 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
     4981 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
     5041 cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg
     5101 catagcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
     5161 gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg
     5221 tttcccctga ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
     5281 aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg gttcgctgga taataatggc
     5341 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
     5401 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
     5461 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
     5521 tcattcagca aagtattctc ggcgttgaca atatgttgcg tgaaaaccaa agaggtgcca
     5581 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcataa
     5641 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga
     5701 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
     5761 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga
     5821 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
     5881 tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa
     5941 tttatgggcc tagagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga
     6001 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa
     6061 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca
     6121 gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc
     6181 aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct
     6241 tgtccattac cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc
     6301 tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag
     6361 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
     6421 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
     6481 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
     6541 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
     6601 ggcatcacaa acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg
     6661 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
     6721 ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
     6781 agccatgcgc cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg
     6841 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
     6901 gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc
     6961 agttcgcccc cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
     7021 ctcaccacgc ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag
     7081 tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct
     7141 acaccaatta ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
     7201 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
     7261 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
     7321 ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag
     7381 tgtggtgcaa tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
     7441 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
     7501 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
     7561 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
     7621 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
     7681 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
     7741 gaattcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
     7801 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
     7861 cgcagaagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg
     7921 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc
     7981 gctacacgcg ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc
     8041 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg
     8101 aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
     8161 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata
     8221 accggggtat ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca
     8281 aatttgttcg tctcctcttc ctgatcagga atgccgaaca aagtaaaggc agcaggcgca
     8341 ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt
     8401 tcgtagccgg attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg
     8461 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
     8521 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg
     8581 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
     8641 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaatcaga
     8701 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
     8761 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
     8821 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
     8881 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
     8941 gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
     9001 ccggagagga cgtagaccgt ttccataatg gccaggagga acgccatacc gagcgtcagt
     9061 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
     9121 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
     9181 aaagggtcac acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga
     9241 atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat
     9301 cccacgtata tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt
     9361 tgcatttttc gacggtgatc aatttatagc taaattgccg cctttcagcc aatttgatcg
     9421 agaacaattt atctcttttt gatgcccatt tccaagactt atacattgat aaatatcaat
     9481 ttctacaaat tatgttaatc atgtttcaac aaggtaactt tagtgacatt tatgtttaaa
     9541 atgtgtgaat tataagactg aaattagttt cggttggcaa ttaaagataa aaagagacga
     9601 agcaaaaaca gcacggttga ccttaattct ttttttggtc actggctaat acgacgcaga
     9661 gtttcaggat tattaataaa ggcataaatc tcctccccgc cgatattaac atttaattaa
     9721 cttataatta tattataatt aactcacccg gggttattat tttgttaaaa ataaagccac
     9781 atatacgtag cttgagagtg aagataaacg caaaattacc tgatgcgcta cgcttgccag
     9841 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
     9901 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
     9961 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
    10021 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10081 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10141 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10201 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10261 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10321 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10381 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10441 ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt ccccagtaag cccacgccgc
    10501 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtagcc
    10561 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag ttgagcatat
    10621 tccataccgg aaccggcgct tctttccagc cttcttgctg ccagttgttc attgcactgt
    10681 cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta tcggtgttaa
    10741 aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    10801 ccacatcaat gcgcctgttg ttatgactaa gagtgacaac caactcgaca cctactctgc
    10861 cagtggattg tcttgcgctg cgttgttgca gattacgcgg caccgccatt tcatagcgga
    10921 taacagccct gctctgccag gcctcatgaa taatctcgca ttgcggtttc gcattcgccg
    10981 aagtaattac ccactcttct tttgcgggtg aatagtcata ttcatcacca tcatcagagc
    11041 tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct accagttgta
    11101 gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc ccttcagcgt
    11161 cactttttgc cgcaatcacg ttgccaggct gattcgcttc gatataaagc gtgcgatagc
    11221 ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga tcgtaattac
    11281 cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca tggcgaataa
    11341 aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg cgcagccgca
    11401 cagtggtgtt gatcacttct tcccgcggcc agggcatcag gttaaacagg acgagtttgt
    11461 cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat atagaaacgc agcagattat
    11521 ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc gcgatgaact ttgtcactac
    11581 agcagcagcc gatgctgtcg tgggcatgat tttttaagat ctctttccat attttctcca
    11641 gcaagccgtg gtggtattca aaacccaacg tccaggccag cgttgccagc ggttccagca
    11701 gattaacaat cttattttca atacgtgcgt gggcaacttt gatatccatg cgcgtggaac
    11761 cgatggtgcg atgcacgcgc atatatttgc catcaataaa ttcccctttc agggttgcca
    11821 gattatctcg ctgcgcttcg atcttctcaa atacctcttc aaagcggctc atcacaaact
    11881 ttcgttgagg ataaatttcg cgcagtttct ccatcacttc gaagatattt tgctgcaatg
    11941 gcatctgatc atgcccattc ggcagcaaaa tctctttggt tacagacgct ttttccagca
    12001 cgtcaaaata actgtcgagg cgtttgcgga atccgttttc gtccgcaggt aagtacttac
    12061 cgatggcata acccaacggc atcacctgcg ccgtcacttc gctaccgtca ctgctttgcc
    12121 acaaaaactc ggttttatcc gtaccgtggc gctcagaaca tccacgccag aacatggtgc
    12181 aggtaatgcc aaatccattg tagatatgcg gcagttgccc ggacatgcca aaggaatcgg
    12241 gtaaataacc tattttcatc ggctcgccaa acgcgaggca gtcacgcatt ccgtacatca
    12301 gattacggac gatggattcc gcagaaacaa tcgtggtatc ggtctgggta taccaggggc
    12361 cgataatcaa cttgccggct tctaccagtt tcttcacgcg gtctttgttt tccggtttca
    12421 ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag tacgtaatat ttgtattcgt
    12481 tgtcctgttc cagtcggcac aggatctctt ccatattatt gaccagcaga atgcgtgact
    12541 cttcggtggt gaaataccac tcccgatccc agtgcatatg cggggtgatg tgaacgcgag
    12601 ataccgcttt catcgtcgtt tcctttttgt ttattgcatt acgccatcag tcagatagtt
    12661 gccatgctta accgcgtgac gccgccagat caggagaatt gcagtcgaga ttgcagcccc
    12721 caccaatgcc gcaccaaacc agccaattgc cgccataaca ccgcccgcgc cattatcatg
    12781 aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa aggccgatat tcatcgcacc
    12841 gacaatagcg cccgttacca tagagcccag cacaaacgaa ccaataaccc gcagcggatc
    12901 ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc agccctaaca gccaggtgga
    12961 tttcccggtc tcaatttcaa actctttaaa caggcgcggt gcgagcatcg tggaagcggt
    13021 tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg gcatacgggc cgtaaacgcc
    13081 gttcgccatt gcgcccaggc agaatgcata agcggcttta ttcactggcc ctccaaggtc
    13141 aaaggaacac ataaaaccga gaatggcacc cagcaacagc gcgttacttc ctgacagacc
    13201 gttcagccag gcggtaagcg agttattgat ccaggcgaca ggttccccca ccacaaacag
    13261 catcagactg cccgctccca atgtaccgag caccaggtag agataaaaag tcaggaagcc
    13321 attgaattta ctgctaagac gcaagtgatt tttcatccag cgtatcaagt aaccggctat
    13381 caatccgcca acgaccgcgc cgagaaaccc ggagccgatc atgttggcgg caagtccagc
    13441 cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13501 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13561 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13621 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13681 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtacggtacg
    13741 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13801 cggctcggca acaggcacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13861 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13921 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13981 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    14041 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14101 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14161 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14221 attgggggga atcgccagca acaccactaa atcaatcgct tccgggccat ccacgctttc
    14281 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14341 tttgccatgc ggcacagcca acccttcacc taaggcggtc gggccaaggc tttcacggcg
    14401 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14461 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14521 gcggtgggtc agagtcgtca ggttcatcat gatcctcact tgatcgtttt tatagtcgcg
    14581 ccagtgtgcc cgataaaaca ataccattgc gtaatcaatt tcacttgatt gaaattattt
    14641 gcattgtatt tgtattattt acaggatgaa tttatcgggc ataatggcta aattcgtatt
    14701 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14761 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14821 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14881 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14941 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    15001 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15061 acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15121 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15181 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15241 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15301 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15361 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15421 gcgaaaatca tcgcgataag cacaaaaaag gccatcatac gatggccaac catgtcgaaa
    15481 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
    15541 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
    15601 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
    15661 atcgcacccg cgtggcccat acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc
    15721 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
    15781 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
    15841 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
    15901 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
    15961 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccgat tttgcattca
    16021 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
    16081 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
    16141 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
    16201 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
    16261 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
    16321 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
    16381 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
    16441 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
    16501 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
    16561 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
    16621 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
    16681 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
    16741 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
    16801 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
    16861 gatcttcctg cgactggtca cgcatttcgc gcagatcagg ctggcggaac agggcgttgc
    16921 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
    16981 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
    17041 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
    17101 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
    17161 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
    17221 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
    17281 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
    17341 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
    17401 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
    17461 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
    17521 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
    17581 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
    17641 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggct
    17701 cttccagcaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
    17761 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
    17821 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
    17881 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
    17941 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
    18001 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
    18061 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
    18121 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
    18181 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
    18241 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
    18301 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
    18361 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
    18421 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
    18481 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
    18541 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
    18601 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
    18661 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
    18721 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
    18781 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
    18841 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
    18901 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
    18961 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
    19021 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
    19081 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
    19141 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
    19201 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
    19261 cttcacgccc ttcggatcaa gttcgtcgat ttcaccgatg gctggcagga aggtgccgtt
    19321 cgccagttct tcgaggctgg aaaccgccag cggatgacgc agcagggatt tcggcgacat
    19381 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
    19441 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
    19501 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
    19561 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
    19621 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
    19681 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
    19741 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    19801 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    19861 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    19921 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    19981 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    20041 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    20101 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    20161 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcgccac
    20221 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    20281 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    20341 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    20401 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    20461 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    20521 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    20581 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    20641 gtggatggtg attggcagca ctttgttgct gctcggctca tcaagtctgt ccagacgggc
    20701 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    20761 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    20821 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    20881 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    20941 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    21001 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    21061 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    21121 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    21181 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    21241 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    21301 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    21361 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    21421 gagctgcagg actttaacct gcttcacatt ggtgtcaggg tcggagatcg ttgaagagta
    21481 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    21541 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    21601 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    21661 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    21721 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    21781 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    21841 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    21901 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    21961 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    22021 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    22081 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    22141 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    22201 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    22261 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    22321 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    22381 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    22441 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    22501 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    22561 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    22621 catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    22681 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    22741 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    22801 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    22861 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    22921 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    22981 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    23041 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    23101 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    23161 gttccaccgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgcgcag
    23221 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    23281 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    23341 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    23401 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    23461 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    23521 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    23581 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    23641 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    23701 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    23761 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    23821 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    23881 aatacgccct gccccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    23941 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    24001 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    24061 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    24121 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    24181 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    24241 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    24301 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    24361 agaaacggta tgggaacggg tcgggaagac tttagagagc agcgcacagg tctggccgct
    24421 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    24481 aaattctctg actggcaatt tcatcacacc ccccacacca caacgaatcc ataaatcacg
    24541 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    24601 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    24661 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    24721 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    24781 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    24841 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    24901 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    24961 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    25021 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    25081 ggctggtacc cagaagccac agcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    25141 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    25201 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    25261 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    25321 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ccataactgc gcgtcatgca
    25381 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    25441 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    25501 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    25561 aatttttaat catctaattt gacaatcatt caacaaagtt gttacaaaca ttaccaggaa
    25621 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    25681 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    25741 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    25801 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    25861 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    25921 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc tcaacgggga
    25981 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    26041 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    26101 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    26161 gatcgatcag ctggcgaccg attctaacta cctagaagtt tgttacatcc tgctgaatgg
    26221 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    26281 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    26341 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    26401 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    26461 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    26521 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    26581 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    26641 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt ttgcctgtat
    26701 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    26761 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    26821 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    26881 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    26941 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    27001 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    27061 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    27121 cgcccactgg agcgaaatgc acagttacgg tatgaagatt gcccgtccgc gtcagctgta
    27181 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    27241 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    27301 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaaatatatc
    27361 ggtataaatt ataaacaaat cgatgatttt taatcatcta tcttcctctc agttacatga
    27421 ataaaccacc aaatatagcg cacccctata atttccaata ctttattcat caaacattta
    27481 attcatacca tcaattaatt acttttacat ttaataaaat taataatatt tttaagaaaa
    27541 actaaaaaca ttcgacaatc aaataagata ttcttgcacc gatatacttt tttcttttct
    27601 gttaaaggac tcagttatgt ttaaaggaca aaaaacattg gccgcactgg ccgtatctct
    27661 gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc actttaaagg
    27721 tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag aggttgaact
    27781 cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg ttgctgtcga
    27841 cctgctctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg gcaagatttc
    27901 taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc cgattctcaa
    27961 caacaccagc acgggtgaag ccacctgcgt cggcgtacgt ttaatgaata aagatcaaag
    28021 caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct ccagcgaaca
    28081 aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg taacgccagg
    28141 cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac tttggcggcg
    28201 ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat ttatcgactc
    28261 tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcgctcga attcaatctg
    28321 aatgttctcg ataaatcaat gcgcgaccgc attgatattt cattattaaa ggaaaaagga
    28381 gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca aatcagtaac
    28441 gagcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat caatgattta
    28501 ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cattaccatt gataaatcag
    28561 tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa
    28621 ctgaatatta gtattccgca agcctggctg gcgtggtact cagaaaactg gaccccaccc
    28681 tccacatgga aagaaggtgt cgccggtgtc ctgatggatt acaacttgtt tgccagcagc
    28741 taccgcccac aggatggcag cagcagcact aatcttaacg cctatggaac agccggaatc
    28801 aacacggggg catggcgctt acgcagtgat tatcagttga atcatactga tagcgatgat
    28861 aaccatgaac agtcaggcga aatatcacgt acctatcttt ttcgcccgtt accgcaatta
    28921 ggttcaaaat taaccctcgg cgaaacggat tttagttcca atattttcga cggtttttct
    28981 tataccggcg cggcactggc aagtgacgat cgaatgttgc catgggaact acgcggctac
    29041 gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag tcaatcaggc
    29101 cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga ccttaatcag
    29161 tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt gaacaatttc
    29221 caggtttcag cagcatcgac gcccttcctg actcgtcagg gacaggttcg ctataaactg
    29281 gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac cttttttagc
    29341 aatgaagttt cctgggggat gctgtcaaac acctcgctgt acagcggcct gctgctttct
    29401 ggtgatgact accattctgc cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg
    29461 ctgtcgtttg atgtcacgtg ggccagtagc cattttgata ctcagcagga cgagcggggc
    29521 ttaagctacc gttttaatta cagcaaacaa gtggatgcca ctaacagcac gatttccctc
    29581 gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct ggatcacaaa
    29641 tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttatctgt gggccaaccg
    29701 attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg gtggaatgca
    29761 gacgcctcca cgaccgccaa catcacggca ggttttaatg ttgatattgg tgactggaga
    29821 gatatctcta tttctacgtc attcaataca acccattacg aagataaaga tcgcgacaac
    29881 cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcaggttgg ctatgacatg
    29941 caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct ggatgaacgt
    30001 aatagctggg gcatgtctgc cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg
    30061 agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg tacctatgcc
    30121 gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc aacccaatat
    30181 ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt cagcaccgat
    30241 ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt tggcattgcg
    30301 gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat gaatgactta
    30361 cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga aggcgcgata
    30421 ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc
    30481 agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg cattagcgtg
    30541 gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt
    30601 accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca tatggaagac
    30661 accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg acatttatga
    30721 aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca ctacaacccg
    30781 atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtctgcgg gtggataatc
    30841 gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa
    30901 gcgatgatct tctggtcgcc ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc
    30961 aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc gaaacgctat
    31021 ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac catgccgttc
    31081 tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca ttacgcaaga
    31141 aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca gcagggcaac caactgacac
    31201 tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat gaaaaaggcg
    31261 ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac acaaacaccg
    31321 gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc gcgttacgca
    31381 tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg aagaaatgag
    31441 tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgccgttga agtctgcgct
    31501 tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag aagcgattca
    31561 accttttgct gtaccaggca atgccaaact cggcgataag atctgggaat ctgacgatat
    31621 taaaattccc gtctattgtg acaacaatac caacggcaac tttgaaagcg aacatgtcta
    31681 cgcatgggta aacccctacc cgggggtgca ggatcgttat tatcaactcg gagtaactta
    31741 caatggcgtt gattatgacg ccaaccaggg aaaaagccgc atcgacacta accagtgtat
    31801 cgacagtaag aacattgaca tttataccct tgagcagatc atcgcgatgg gatggcagaa
    31861 caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat
    31921 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
    31981 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa
    32041 cctgaaatat catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
    32101 ttccatttca ccggaaacac aggtgattga ttttggtaaa tttaatttgc tggatattcg
    32161 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
    32221 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
    32281 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
    32341 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
    32401 aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctggggccct
    32461 tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag
    32521 ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta
    32581 ttttttccta gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg
    32641 gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct
    32701 attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg
    32761 atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg
    32821 caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg
    32881 gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc
    32941 gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct
    33001 tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc
    33061 ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg cgggtgccgc tgttctggtc
    33121 acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg
    33181 ccagtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga
    33241 tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc
    33301 cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg
    33361 gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc
    33421 ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc aggtatggcg
    33481 tggggactga cccggtttat gcatattgat tttatgactg cctacctcgc caccagtccc
    33541 ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt
    33601 atggcgatgc aaaccctgcg cctgttcagt attttgctga cagggccagc cattgcgcgg
    33661 tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa
    33721 aacgggcgcg aggacaacgt ggttttctca atgatgccgc cacagcgttc gcacagttcg
    33781 ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc
    33841 tgcccccgcg tagcgtaaga aaaccgagga atctccagta acgcgtgtgc gagtgcatcc
    33901 agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg
    33961 atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct
    34021 gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt cctttaccac ctccggcgtc
    34081 agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt
    34141 tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt
    34201 tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc
    34261 cagacgccgt aaagctgatt atggctgtag agcgttaaac cgttgggaaa atgagttaac
    34321 aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa
    34381 gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc
    34441 tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt
    34501 cattctttat taagcggggt tatgctgcga caacaatccc ctttttggca aatgtagctc
    34561 gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg
    34621 ccgcgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcca
    34681 gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctggctga cttcgcggca
    34741 ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc
    34801 catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga accaatgccg
    34861 gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct
    34921 ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa
    34981 tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttcc ggcggcagct
    35041 gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga
    35101 caccattttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa
    35161 atccacaagc aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc
    35221 cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttca
    35281 aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa
    35341 tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag
    35401 gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg
    35461 ttatgcggca cctgcaccac gcccggtaac aaaccgtgag ataacagttc gcgatcggtg
    35521 atgcgtttta aaatttgccc ctgtaaacga tagcccattc ggttactttg cgagctaagc
    35581 tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc
    35641 cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata
    35701 aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa acggccttcc
    35761 agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca
    35821 ataccacccg cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa
    35881 cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcgttatc atccaaccgc
    35941 gcttcgcaac ctgcacccgt cagggcaaac cacccatcag tttcgaactc aactgttaac
    36001 tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca
    36061 atgcgcagcg caggcatatc cagtgcgcca cagtggctga tacccgactg gcgaaaaccg
    36121 tgacgaccgc catcctgcac agtggtgtac atgcccgcac gaataatctt cagcatattc
    36181 cctccttctg cggtacaaat cgcacgctgt ctcccggacg taataagatg ggttcgtcac
    36241 gcgccggatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg
    36301 ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca
    36361 agcgcggttc agcgcgccgt ggcgtgtgtt gttgttccgg caaactcccg agatacggga
    36421 agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa
    36481 cctgtttttc gctcaacccg caatacgccg cgaccaccgc caaatccggt ccgcctgcac
    36541 caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct
    36601 cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa
    36661 tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac
    36721 gctgcgccag tcgccagatc cgcttctggc tagccagcgt caccggcggt tccagttcca
    36781 gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg
    36841 attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca
    36901 ttcagccact cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg acctgcagca
    36961 taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta
    37021 ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg
    37081 caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt
    37141 cccagacgcg cttcaatcca ggaggctagt tccattcccg gcacaggcat ggtcagctct
    37201 ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc
    37261 tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg
    37321 atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac
    37381 tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag acgcactgcc
    37441 tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg
    37501 ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg
    37561 ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta
    37621 caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg acatattaag
    37681 aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat
    37741 atttatgcaa tcagacagca tattgcataa ataatcagtt ctgtatcaaa gttttaactt
    37801 taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta
    37861 ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct
    37921 gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac
    37981 taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg
    38041 tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga
    38101 tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc
    38161 cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta
    38221 acgtaagctg tctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt
    38281 tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt
    38341 acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtt ggcatgatcg
    38401 cgggtctggt cattttctta tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca
    38461 aaaaagtact gcgtgcgaca aactttctcc tgccgaactg gggatggctg ctggttctgc
    38521 tggtggcaac gcctgcactg attaccgtgc tgttctggaa agagtggtcg gtatacgcct
    38581 taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc aaagcagaaa
    38641 accagaaaca gcggaaggag ctggggctga ttgtgacgct caccttattc agtatgttgt
    38701 tctgggcctt cgcacaacag ggcggtagct cgattagcct ttatatcgac cgcttcgtta
    38761 accgcgatat gtttggttat accgttccga ccgcgatgtt ccagtcgatt aatgccttcg
    38821 cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc
    38881 gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct
    38941 gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga
    39001 tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt
    39061 cgcaaattac gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc
    39121 tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg
    39181 cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg
    39241 atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc
    39301 aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg
    39361 cctgatgcga cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat ttattgaatt
    39421 tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat ccggcatctg ggctcgattg
    39481 cctgacgcgt ctgttatttc cccttccgcg ccgtctcata cgctgccaac gtttgtactc
    39541 tcgcctcttt gtgctcgact atcggttgcg gataatccag cttcacacct gctttctgcg
    39601 cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc
    39661 gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatagga ttgaaaatac
    39721 gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca ccgttattag
    39781 ctgccaaatc accatcaatc agctgcgaca taaaatatcg ctcgccttcg cgccagtcga
    39841 tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta tgcatccagc
    39901 cagtgctgtt aagctgacgc atagcggcat caataatcgg gtatcccgtt ttgcctttct
    39961 gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc caggcaataa
    40021 atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc
    40081 agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg
    40141 ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccaag ctggcggaca
    40201 aacggctggt gccttccact gccggaaaat ctcgttgttg ctcatactct ccagcaccgt
    40261 tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag
    40321 tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggcttt atcgatccgc
    40381 tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc
    40441 aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc atcaccgccc
    40501 ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt
    40561 caacgtccac atcccgcgct cgctcattca cctcatactg atagttataa aaaagatgag
    40621 taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca
    40681 cttcacggaa caataaagga ataccttttt ccgccagcgc gatttgtagc ccattcagtt
    40741 gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtctcccac tggcgtggtg
    40801 tagcgatata cagcgccagc acgc
//
LOCUS       DADLTI010000014        38753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLTI010000014 DADLTI010000000
VERSION     DADLTI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.014"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JN755_000716"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JN755_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7379613.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..579
                     /gene="hisL"
                     /locus_tag="JN755_000717"
     CDS             529..579
                     /gene="hisL"
                     /locus_tag="JN755_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7379614.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            725..1624
                     /gene="hisG"
                     /locus_tag="JN755_000718"
     CDS             725..1624
                     /gene="hisG"
                     /locus_tag="JN755_000718"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7379615.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1630..2934
                     /gene="hisD"
                     /locus_tag="JN755_000719"
     CDS             1630..2934
                     /gene="hisD"
                     /locus_tag="JN755_000719"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7379616.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2931..4001
                     /gene="hisC"
                     /locus_tag="JN755_000720"
     CDS             2931..4001
                     /gene="hisC"
                     /locus_tag="JN755_000720"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7379617.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4001..5068
                     /gene="hisB"
                     /locus_tag="JN755_000721"
     CDS             4001..5068
                     /gene="hisB"
                     /locus_tag="JN755_000721"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7379618.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5068..5658
                     /gene="hisH"
                     /locus_tag="JN755_000722"
     CDS             5068..5658
                     /gene="hisH"
                     /locus_tag="JN755_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7379619.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5658..6395
                     /gene="hisA"
                     /locus_tag="JN755_000723"
     CDS             5658..6395
                     /gene="hisA"
                     /locus_tag="JN755_000723"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7379620.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6377..7153
                     /gene="hisF"
                     /locus_tag="JN755_000724"
     CDS             6377..7153
                     /gene="hisF"
                     /locus_tag="JN755_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7379621.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7147..7758
                     /locus_tag="JN755_000725"
     CDS             7147..7758
                     /locus_tag="JN755_000725"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7379622.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JN755_000726"
     CDS             complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JN755_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7379623.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JN755_000727"
     CDS             complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JN755_000727"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7379624.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JN755_000728"
     CDS             complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JN755_000728"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7379625.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11998..12357)
                     /locus_tag="JN755_000729"
     CDS             complement(11998..12357)
                     /locus_tag="JN755_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379626.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12354..12906)
                     /locus_tag="JN755_000730"
                     /pseudo
     CDS             complement(12354..12906)
                     /locus_tag="JN755_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13007..13606)
                     /locus_tag="JN755_000731"
     CDS             complement(13007..13606)
                     /locus_tag="JN755_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379627.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="JN755_000732"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="JN755_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="JN755_000733"
     CDS             complement(14779..15153)
                     /locus_tag="JN755_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7379628.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JN755_000734"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JN755_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7379629.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17194)
                     /locus_tag="JN755_000735"
     CDS             complement(16325..17194)
                     /locus_tag="JN755_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7379630.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="JN755_000736"
     CDS             complement(17223..18113)
                     /locus_tag="JN755_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7379631.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGV
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JN755_000737"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JN755_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7379632.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JN755_000738"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JN755_000738"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7379633.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JN755_000739"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JN755_000739"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7379634.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JN755_000740"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JN755_000740"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7379635.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JN755_000741"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JN755_000741"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7379636.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JN755_000742"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JN755_000742"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7379637.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JN755_000743"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JN755_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7379638.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JN755_000744"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JN755_000744"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7379639.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JN755_000745"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JN755_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7379640.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JN755_000746"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JN755_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7379641.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JN755_000747"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JN755_000747"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7379642.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JN755_000748"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JN755_000748"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7379643.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JN755_000749"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JN755_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7379644.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JN755_000750"
     CDS             complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JN755_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7379645.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35472..35951)
                     /locus_tag="JN755_000751"
     CDS             complement(35472..35951)
                     /locus_tag="JN755_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7379646.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JN755_000752"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JN755_000752"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7379647.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JN755_000753"
     CDS             complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JN755_000753"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7379648.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JN755_000754"
     CDS             complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JN755_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7379649.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38633..>38753)
                     /locus_tag="JN755_000755"
     CDS             complement(38633..>38753)
                     /locus_tag="JN755_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY7379650.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10349 a         9688 c         8721 g         9995 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
      601 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
      661 ttccgggggc ttttttattg cgcggttgtt aacggttcag acaggtttaa agaggaataa
      721 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
      781 tgactcacgc gaattactgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
      841 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg atgacattcc
      901 cggtctggta atggacggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga
      961 agagctgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct
     1021 ggatttcggc ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggtcc
     1081 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     1141 cgaccagaaa ggtatctctt ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc
     1201 gcgtgccggg ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     1261 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
     1321 tggcgaaatg gaagaatcta aacagcaact gatcgacaag ctactgaccc gtattcaggg
     1381 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctaga
     1441 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
     1501 tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc ctgttctggg aaacgatgga
     1561 aaaactgaaa gcactgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
     1621 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     1681 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg caccgttaac
     1741 gatattctcg ataacgtgaa aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag
     1801 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc
     1861 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagag tattgaaacc
     1921 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     1981 cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg
     2041 ctcttctcaa cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg
     2101 gtgctgtgct caccgccgcc gattgccgat gaaattcttt atgcggcgca gctgtgcgat
     2161 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     2221 gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagca
     2281 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     2341 gaagtgctgg tgattgctga cagcggcgct acgccggatt ttgtggcttc tgatttgctt
     2401 tctcaggctg aacacggccc tgactcacag gtaattttac tgacccccga tgctgatatg
     2461 gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag aactgccgcg tgccgaaacc
     2521 acccgccagg cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgtg
     2581 gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
     2641 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
     2701 gcaggcgact acgcctccgg caccaaccac gtgctgccga cttacggtta caccgccacc
     2761 tgttccagcc tcgggctggc agatttccag aaacgcatga ccgtacagga actgtcgaaa
     2821 gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc
     2881 gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg
     2941 tgactattac tgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     3001 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg
     3061 agtttaagct tactcagcaa acgctcaacc gctacccgga atgccagcca aaagcggtga
     3121 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg
     3181 cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa gacgccatcc
     3241 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat
     3301 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     3361 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa ccccaccggg caactgatca
     3421 atccgcagga ttttcgcacc ctgctggagc taacccgcgg taaggcgatt gtggttgccg
     3481 atgaagctta tatcgagttt tgcccgcagg catcgttagc tggctggctg gcggaatatc
     3541 cgcacctggc gattttgcgt accctttcga aagccttcgc gttggcgggg cttcgttgcg
     3601 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc cccccctacc
     3661 cgctctcgac gccggttgcc gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg
     3721 ccatgcgcga acgggtggcg caaattatta cagaacgcga atacctgatt gccgcactga
     3781 aagagatccc ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct
     3841 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     3901 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     3961 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     4021 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     4081 tgacaaactc gcctttgaac cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg
     4141 ctacaagctg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaagc
     4201 ggattttgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     4261 tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa
     4321 agtaaaactg gtggagggtt atctggctga gcaagcgatg gatcgtgcca acagttatgt
     4381 aattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     4441 ctacgaccgc gaaaccctga actggccaat gattggcgag caactcacta aacgagaccg
     4501 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgtccagg tgtggctgga
     4561 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcaca tgctggatca
     4621 gatcgccacc cacggcggtt tccgcatgga aatcaatgtc aaaggcgacc tctatatcga
     4681 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct
     4741 cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     4801 ccgctgcgca ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
     4861 ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac ttcttccgtt cgctctctta
     4921 caccatgggc gtgacactac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     4981 aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac
     5041 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
     5101 aacctgaact ctgtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
     5161 gacccggaca ttgtgttgct ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa
     5221 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     5281 ccggtgctgg gtatctgctt agggatgcaa ctcctgggac gacgcagcga agagagcaat
     5341 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
     5401 ccactgccac atatgggctg gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag
     5461 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     5521 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
     5581 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     5641 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     5701 gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct
     5761 tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct ggtggatctg accggggcaa
     5821 aagatccggc taagcgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc
     5881 cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg
     5941 gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc accggacgta gtgaaaggct
     6001 ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact ggatgtccgt attgacgagc
     6061 aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa ctcgggcatt tcactggaac
     6121 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc
     6181 gcgacggcac gctggcggga tctaacgtct ctttatatga agaagtgtgt gccagatatc
     6241 cgcaagtggc gtttcagtcc tccggcggca ttggcgacat taacgatgtt gctgccctac
     6301 gtggcactgg cgtacgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg
     6361 tgaaggaggc catcgcatgc tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg
     6421 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccact
     6481 ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg ttctacgata tcaccgcttc
     6541 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     6601 tccgttttgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt
     6661 tggcgcggat aaaatttcca tcaactcccc tgcgctggca gacccaacat taattactcg
     6721 cctggccgat cgctttggcg tgcagtgtat tgtggttggt attgatacct ggtacgacgc
     6781 cgaaaccggt aaatatcatg taaatcaata taccggcgat gaaagccgca cccgcgtcac
     6841 tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt
     6901 cctcaacatg atgaatcagg acggcgtgtg taacggttac gacctcaagc agctgaaaaa
     6961 agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca ccatggaaca
     7021 cttcctggaa gccttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
     7081 caaacaaata atcaatattg gcgaattaaa agcgtacctg gcaacacagg gcgtggagat
     7141 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggtctg
     7201 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctacatgaac
     7261 ccggaagcct tagacaaaac catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa
     7321 cagcgactgt ggaccaaagg cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc
     7381 ccggactgcg acaacgacac attactggtg ctggcgaatc ccatcgggcc aacctgccac
     7441 aaaggcacca gtagctgctt cggcaacacc gctcaccagt ggctgttcct gtatcaactg
     7501 gaacaactgc tcgccgaacg taaatatgcc gacccggaaa cctcctacac cgccaaactg
     7561 tatgccagcg gcactaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
     7621 ctggcagcaa cggtacatga tcgctttgag ctgaccaacg aggcatctga cttgatgtat
     7681 cacctgctgg tgctgttgca ggatcaggat ctggatttaa cgacagtaat tgagaacctg
     7741 cataaacggc atcagtgagt tacgggctga gcaggtgtga tgttgttgtc acacccggag
     7801 ccattgcccg atgcgcaaac ttatcgtgcc tacacattat ctcaacacac gtaggccgga
     7861 taagatgcgc cagcatcgca tccggcaaaa aaaacgggca tggtgtcacc accctgcccg
     7921 tttctcttaa atgcacaata atattacttc gcgttgtaat tacgcagagc gttacgcccc
     7981 aacacaatcc ccgcgccaac catgccgccc agaagcactg ccagaatcaa ggtgattgcc
     8041 tttttcggac tatcgcgacg aataggtaac gtcggtttca tcacatagcg gtaagcatga
     8101 atatcaagat catcaaactt taatttttca atatccaaca ggttttgacg tgtctgatag
     8161 tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga ctccagcgct
     8221 tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt cagtctgctg aacctgtggc
     8281 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
     8341 tctttctgct cctgcgcgac cacttcctgg gttcttaaag agtcctgcaa gtttttccgt
     8401 cccagagcaa tgttgtcctt gagatccttt tctagctctt gattcacttt atcatcaact
     8461 tgctgaatgt attgggccaa cttcatttgt gcgccctctg cagtttgccc aacataagaa
     8521 acagtcaatg gtaattgttg gttcttaaca gaaggttcga tggtaagttt ttctggctct
     8581 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
     8641 gtctcctgca aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
     8701 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
     8761 tccttcgcta ctgccaaata accaatagcc agggcaatag ccacaatgac ggaaatgata
     8821 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
     8881 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgaa
     8941 aatttccaag tccgcatagt ttagcgataa tccccgtgaa tgctatagga taagtgataa
     9001 aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
     9061 aagatgttag tcgctgccaa agagatcgcg ggtgtagacc ttatctgcca catccttaag
     9121 ctcttctgcc atacggttgg agataatgac gtcggcttgt tgtttgaagg tggcgagatc
     9181 acgttccagg cgagagttga agaatgagtc ttctttcatc accggctcgt agatgatcac
     9241 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg tacggaagtt
     9301 atctgacccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
     9361 aatggcatcg gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgagatcag
     9421 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     9481 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     9541 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     9601 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     9661 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     9721 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9781 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9841 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9901 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9961 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    10021 gtagtcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    10081 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    10141 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    10201 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    10261 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
    10321 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
    10381 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
    10441 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
    10501 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
    10561 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
    10621 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
    10681 cgcctgctgg tagtcatcgg caatttgctt gaagtacggt gccagcagca ggttagcgat
    10741 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10801 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    10861 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10921 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10981 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    11041 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    11101 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    11161 atcttttttg gtgaagatat ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt
    11221 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    11281 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    11341 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    11401 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    11461 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    11521 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    11581 ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggtttca gggaatcaat
    11641 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    11701 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    11761 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11821 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11881 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11941 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    12001 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    12061 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    12121 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    12181 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    12241 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    12301 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    12361 ttcagaataa aaatatttga attcaaaatg caacgatcgc cccgaaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagttcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 accccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag ttcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt aaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg tttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatagctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caatcatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gctgcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaaatt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgaca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgtgtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattac gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg tgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 tgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    35281 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    35341 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    35401 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    35461 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    35521 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    35581 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    35641 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    35701 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35761 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35821 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35881 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35941 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    36001 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    36061 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    36121 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    36181 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    36241 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    36301 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    36361 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    36421 acaggttggt cggcatgaca gagcggtaat cgcgtccata ctggcggttg taagattcgc
    36481 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    36541 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    36601 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    36661 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36721 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36781 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36841 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36901 aaatgcgttg tttacttatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36961 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    37021 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    37081 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    37141 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    37201 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    37261 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    37321 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    37381 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    37441 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    37501 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    37561 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    37621 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    37681 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37741 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    37801 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37861 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37921 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37981 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    38041 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    38101 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    38161 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    38221 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    38281 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    38341 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    38401 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    38461 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    38521 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    38581 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    38641 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    38701 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gca
//
LOCUS       DADLTI010000015        37643 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLTI010000015 DADLTI010000000
VERSION     DADLTI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.015"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..174)
                     /locus_tag="JN755_000756"
     CDS             complement(<1..174)
                     /locus_tag="JN755_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7379651.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JN755_000757"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JN755_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7379652.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JN755_000758"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JN755_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7379653.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JN755_000759"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JN755_000759"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7379654.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JN755_000760"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JN755_000760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7379655.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JN755_000761"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JN755_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7379656.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JN755_000762"
     CDS             complement(5178..5420)
                     /locus_tag="JN755_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7379657.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JN755_000763"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JN755_000763"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7379658.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JN755_000764"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JN755_000764"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7379659.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JN755_000765"
     CDS             complement(8337..9311)
                     /locus_tag="JN755_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7379660.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JN755_000766"
                     /pseudo
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JN755_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
     gene            11708..12751
                     /locus_tag="JN755_000767"
     CDS             11708..12751
                     /locus_tag="JN755_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7379661.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JN755_000768"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JN755_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7379662.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JN755_000769"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JN755_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7379663.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JN755_000770"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JN755_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7379664.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JN755_000771"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JN755_000771"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7379665.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JN755_000772"
     CDS             16537..20337
                     /locus_tag="JN755_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7379666.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JN755_000773"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JN755_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7379667.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JN755_000774"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JN755_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7379668.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JN755_000775"
     CDS             23178..23381
                     /locus_tag="JN755_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7379669.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JN755_000776"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JN755_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7379670.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JN755_000777"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JN755_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7379671.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JN755_000778"
     CDS             26386..26658
                     /locus_tag="JN755_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379672.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JN755_000779"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JN755_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7379673.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JN755_000780"
     CDS             complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JN755_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7379674.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28247..29185
                     /gene="mdh"
                     /locus_tag="JN755_000781"
     CDS             28247..29185
                     /gene="mdh"
                     /locus_tag="JN755_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7379675.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JN755_000782"
     CDS             complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JN755_000782"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7379676.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JN755_000783"
     CDS             complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JN755_000783"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7379677.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31925..32323)
                     /locus_tag="JN755_000784"
     CDS             complement(31925..32323)
                     /locus_tag="JN755_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7379678.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32517..33644
                     /locus_tag="JN755_000785"
     CDS             32517..33644
                     /locus_tag="JN755_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7379679.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33863..34291
                     /gene="rplM"
                     /locus_tag="JN755_000786"
     CDS             33863..34291
                     /gene="rplM"
                     /locus_tag="JN755_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7379680.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34307..34699
                     /gene="rpsI"
                     /locus_tag="JN755_000787"
     CDS             34307..34699
                     /gene="rpsI"
                     /locus_tag="JN755_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7379681.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35094..35732
                     /gene="sspA"
                     /locus_tag="JN755_000788"
     CDS             35094..35732
                     /gene="sspA"
                     /locus_tag="JN755_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7379682.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35738..36235
                     /gene="sspB"
                     /locus_tag="JN755_000789"
     CDS             35738..36235
                     /gene="sspB"
                     /locus_tag="JN755_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7379683.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JN755_000790"
     CDS             complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JN755_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7379684.1"
                     /translation="MGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGVLDPAKSSGYL
                     IVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYI
                     ILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGI
                     LETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQA
                     EQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEF
                     FRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSG
                     LGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATT
                     LARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9115 a         9416 c         9902 g         9210 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgtggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaaa ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac tagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagcgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga tgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accttggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctagcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcactgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg atatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttgacggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttagtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccctt
    26461 ggtctggcta aagataaggt acgcgatctt gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 aaacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gtatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agcatacact aattattgaa
    27061 ctaattaata ccacgctaac tgtttcagtt gatactaagt gttaaaaatg tgaattttaa
    27121 taacaaaaaa gtataaaaaa cacggttatt tattattaat tatagtattt tcaattagat
    27181 aatgaatttt gcataatgtg ctttttttac cgtatgtatt cattttgtga atgatgtgtc
    27241 gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa agaaggggaa tgagagggat
    27301 aagcaacatt ttccccgccg ccagaagcga cggggcagag attaaagctc ctggtcgaac
    27361 agctctaaaa tcgcttcgta caggtctttg acggtgaaac cgttagcagg ggtggtaaag
    27421 atggtgtcat cgccagcgat ggtgcccaga ataccttctg ctttgcccag tgagtccagc
    27481 aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg
    27541 tagtcgatat ccagcaccag attcttcaat ggactggagg tggttggtac acccagttca
    27601 gctggcaggc agtaaaccat ttccattttg gcattgcgtg tacgtacagc accaaacttg
    27661 gtcaacatcc gcgagacttt agactgatta atattgtcaa agccttgctc ctgcaacgcg
    27721 gcgacgattt cgccctggga gctaaatttc tcttctttaa gtaatgcttt aaatgcttta
    27781 actagttctt cttgcttagc cgagcttcgc ataagtcacc cgatatggtg gttgatacaa
    27841 cattattgtg catacagatg aatttttatg caaacagtca gccctgaaga aggctgaaat
    27901 aatgttatga aagagcggga ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac
    27961 ggcatgcaaa ttctgtttaa aagtaaatta attgttatca aattgatgtt gttttggctg
    28021 aacggtaggg tatattgtca ccacctgttg gaatgttgcg ctaatgcata agcgactgtt
    28081 aattacgtaa gttaggttcc tgattacggc aattaaatgc ataaacgcta aacttgcgtg
    28141 actacacatt cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc
    28201 agcggagcaa catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt
    28261 cctcggcgct gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc
    28321 ttcaggttca gaactctctc tgtatgatat cgctccagtg actcccggtg tggccgtcga
    28381 tctgagccat atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc
    28441 ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat
    28501 ggatcgttcc gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt
    28561 tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt
    28621 tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg
    28681 cgttaccacg ctggatatca ttcgttccaa cacctttgtt gcggaactga aaggcaaaca
    28741 gccaggcgaa gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct
    28801 gctgtcacag gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg
    28861 tatccagaac gcgggtactg aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct
    28921 gtctatgggc caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga
    28981 acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt cagtacgccc gtttcttctc
    29041 tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag
    29101 cgcatttgaa cagaacgcgc tggaaggtat gctggatacg ctcaagaaag atatcgccct
    29161 gggcgaagag ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
    29221 tccggttttt gttttgagca ctcgacttaa ttggttgccg gatattcctg aatggtgacc
    29281 tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga accagggcga
    29341 atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt atccaccgaa
    29401 ataatcagat cgttgacctg aatacccgca ttcgccgctg ggccgtcagg tgacacttca
    29461 ttaaccacga tcccttgcag ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc
    29521 tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat cagcttatcc
    29581 ataattttgg ttgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
    29641 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttctcccag cgagttcacc
    29701 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc
    29761 ccggtcgggt tcagaccgat tcgacccgtg gcactaataa tcccttgggt aatggtctgc
    29821 ccgaggttgt acgggttacc gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt
    29881 gcattaattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac cgccagatcg
    29941 gttagagagt cagatcccac cagcaatgct tcaaatacac gtccatcctg taaggcgacg
    30001 atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc gcgttgatcc
    30061 atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
    30121 ccacggttgt aaacgttaac caccgctggc gcggcgcggc gaaccgccag attatagctg
    30181 gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc
    30241 gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac ggaacgtaag
    30301 agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc ataccacgag
    30361 ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc atcagcagat
    30421 agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt gccgccagca
    30481 ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg atcacatcgt
    30541 ctttttgcaa gccagcctga gcagctgggc ttcctttgac aacttcatct atcttaatac
    30601 ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttccagc gctggcgtga
    30661 tcatttcagc gctggccgac gaagaggtgt tggtatcaag cgtcacttct acttccagtg
    30721 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30781 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30841 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30901 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30961 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    31021 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    31081 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    31141 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    31201 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    31261 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    31321 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    31381 gtttttcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    31441 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    31501 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31561 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31621 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31681 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31741 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31801 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31861 agattaaatg atagtcgtgg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31921 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31981 gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    32041 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    32101 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    32161 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    32221 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    32281 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    32341 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    32401 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    32461 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    32521 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32581 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32641 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32701 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32761 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32821 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32881 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32941 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    33001 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    33061 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    33121 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    33181 atctgtggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    33241 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    33301 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    33361 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    33421 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    33481 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33541 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33601 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33661 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33721 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33781 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33841 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33901 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33961 tcgtcgcctg cgtggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    34021 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    34081 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    34141 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    34201 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    34261 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    34321 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    34381 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    34441 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    34501 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34561 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34621 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34681 gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc
    34741 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34801 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34861 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34921 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34981 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    35041 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    35101 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    35161 atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa
    35221 aggacaatcc gcctcaggat ctgattgacc ttaacccgaa tcagagcgtt ccgaccctgg
    35281 tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg gatgagcgtt
    35341 tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca
    35401 tgcatcgcat cgaaaaagac tggtacacgc tgataaacac tatcatcaac ggttcagctt
    35461 ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt gcgccggtct
    35521 tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc taccttgctc
    35581 cgctgctgtg gcgtctgccg caactgggca tcgagttcag cggcccgggt gcgaaagagc
    35641 tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct ttaactgaag
    35701 cagaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac agctaacacc
    35761 acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc agctcacgcc
    35821 gcacttggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga
    35881 cgggcaaatc gtactcaaca ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga
    35941 tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg tgccgctggc
    36001 tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc
    36061 tgcctacgat gaagatacca gcatcatgaa tgatgaagag gcatcggcag acaacgaaac
    36121 cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc atcctgacga
    36181 tgaacctccg cagccaccac gcggtggtcg accggcatta cgcgttgtga agtaatacaa
    36241 aacaggccca ggcggcctgt tttgtctttt taatgactta ataaaatagc gtgattgtgg
    36301 caatcatatt caccacgaaa ccgactgcca tggggatcgc tgtccgcttc accaccgcaa
    36361 aaggcgaaac gcctgcaatt cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg
    36421 cgcgcgctag cgtcgtggca aaatgcatcg gcataatcat tacaaccgct ggtacatgta
    36481 gtccggctgc gatattcgga ataagactgg caaatgacat aaacggcgca ttgccagagc
    36541 ccatcacaat ggcacaaatg gcaatgacta gcgccataat aatcatcacg ccaataccgc
    36601 ccagaccaga atgctccgca cccctgataa ccgcatcaac agtgccaatc gtcgttaagc
    36661 ctttcgcaaa tatttcaccc gcgaccacca gcgttaccac gttgacaaac tgcgtaccca
    36721 tgccgtcaaa aaacgcctgc acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga
    36781 actcaacaaa catcgtcacc gtcaaactta gtaacatcac caccaccaga tgcagttctg
    36841 acttcatcag cccgacgtgg gcgaggaaca gcgagccgag catcaggatt aacggcatca
    36901 caggtaaaat ggcgtaatag agcggcggga cattatcgag agttttttgc tctgcctgtt
    36961 cgtgattgat atttttatct tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg
    37021 ccaccgagat aatgacgcaa gaggcgaccg gaagctggta gtggaagaag tatgtggcaa
    37081 ttttcattcc cgcgacctgg gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa
    37141 tggacatcgt ggttgcgata accgccaccg cagagaggcg acttactccc agactcacca
    37201 gcgtcggaaa taaggtgacc atcagcaaca tacccagacc ggaggcgctg gtaataaact
    37261 gcgccatgat ttggccgatg acgtaagttg ccgacagaat aatatacggc gagcgaatga
    37321 gttttaacgg gcggcttagc aagctcacca tcgcgcgact ggctcctatg cgctccatgt
    37381 agcgggcata accgcccacc gccataatcg acagccccaa tccggcaatg cggttggaca
    37441 gcatgcgcag gatttcatta taaatgtcga cgatcaagta gccgctgctt ttggcgggat
    37501 cgagtacacc accgaaccct aaccagactc cgcacatcat caggaagaca cctgcggcag
    37561 ccagcaccac ctgaggtttg tagtttttca ggatcaaata gcccatcgta attaatacga
    37621 tgacagatat aattatgccg aac
//
LOCUS       DADLTI010000016        37345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLTI010000016 DADLTI010000000
VERSION     DADLTI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.016"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JN755_000791"
     CDS             complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JN755_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7379685.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(308..1021)
                     /locus_tag="JN755_000792"
     CDS             complement(308..1021)
                     /locus_tag="JN755_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7379686.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JN755_000793"
     CDS             complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JN755_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7379687.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2464..2994
                     /gene="rppH"
                     /locus_tag="JN755_000794"
     CDS             2464..2994
                     /gene="rppH"
                     /locus_tag="JN755_000794"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7379688.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3007..5253
                     /gene="ptsP"
                     /locus_tag="JN755_000795"
     CDS             3007..5253
                     /gene="ptsP"
                     /locus_tag="JN755_000795"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7379689.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5404..6279
                     /gene="lgt"
                     /locus_tag="JN755_000796"
     CDS             5404..6279
                     /gene="lgt"
                     /locus_tag="JN755_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7379690.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6286..7080
                     /gene="thyA"
                     /locus_tag="JN755_000797"
     CDS             6286..7080
                     /gene="thyA"
                     /locus_tag="JN755_000797"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7379691.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7264..7734
                     /locus_tag="JN755_000798"
                     /pseudo
     CDS             7264..7734
                     /locus_tag="JN755_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7725..8288
                     /locus_tag="JN755_000799"
     CDS             7725..8288
                     /locus_tag="JN755_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7379692.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8285..8692
                     /locus_tag="JN755_000800"
     CDS             8285..8692
                     /locus_tag="JN755_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7379693.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8677..9000
                     /locus_tag="JN755_000801"
     CDS             8677..9000
                     /locus_tag="JN755_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7379694.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9013..12381
                     /gene="recC"
                     /locus_tag="JN755_000802"
     CDS             9013..12381
                     /gene="recC"
                     /locus_tag="JN755_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7379695.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12557..15445
                     /gene="ptrA"
                     /locus_tag="JN755_000803"
     CDS             12557..15445
                     /gene="ptrA"
                     /locus_tag="JN755_000803"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7379696.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15438..18980
                     /gene="recB"
                     /locus_tag="JN755_000804"
     CDS             15438..18980
                     /gene="recB"
                     /locus_tag="JN755_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7379697.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18980..20806
                     /gene="recD"
                     /locus_tag="JN755_000805"
     CDS             18980..20806
                     /gene="recD"
                     /locus_tag="JN755_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7379698.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JN755_000806"
     CDS             complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JN755_000806"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7379699.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22431..23684
                     /locus_tag="JN755_000807"
     CDS             22431..23684
                     /locus_tag="JN755_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7379700.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23759..23835)
                     /locus_tag="JN755_000808"
     tRNA            complement(23759..23835)
                     /locus_tag="JN755_000808"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23799..23801),aa:Met,seq:cat)
     gene            24044..25141
                     /gene="mltA"
                     /locus_tag="JN755_000809"
     CDS             24044..25141
                     /gene="mltA"
                     /locus_tag="JN755_000809"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7379701.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25380..26186
                     /gene="tcdA"
                     /locus_tag="JN755_000810"
     CDS             25380..26186
                     /gene="tcdA"
                     /locus_tag="JN755_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7379702.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="JN755_000811"
     CDS             complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="JN755_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7379703.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="JN755_000812"
     CDS             complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="JN755_000812"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7379704.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28077..28304
                     /locus_tag="JN755_000813"
     CDS             28077..28304
                     /locus_tag="JN755_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7379705.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28655..29572
                     /locus_tag="JN755_000814"
     CDS             28655..29572
                     /locus_tag="JN755_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7379706.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29591..29986
                     /locus_tag="JN755_000815"
     CDS             29591..29986
                     /locus_tag="JN755_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7379707.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            29979..31079
                     /gene="rlmM"
                     /locus_tag="JN755_000816"
     CDS             29979..31079
                     /gene="rlmM"
                     /locus_tag="JN755_000816"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7379708.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="JN755_000817"
     CDS             complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="JN755_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7379709.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="JN755_000818"
     CDS             complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="JN755_000818"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7379710.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="JN755_000819"
                     /pseudo
     CDS             complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="JN755_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="JN755_000820"
                     /pseudo
     CDS             complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="JN755_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(35672..36971)
                     /gene="fucP"
                     /locus_tag="JN755_000821"
                     /pseudo
     CDS             complement(35672..36971)
                     /gene="fucP"
                     /locus_tag="JN755_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
BASE COUNT         8956 a         9263 c        10072 g         9054 t
ORIGIN      
        1 ttaccttgct gatcgtgata actcaccagc ccggtatcat catcaatttc aggttttcca
       61 tcggtcaaaa tcatacggcc atctttggtc gccatgacgt aatcactgga acacccggaa
      121 acagcaaacg ccagtccgac tgcagaaatt attactgccc attttttcat ccggttatcc
      181 tcatttggcc tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc
      241 aggaaaatct taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta
      301 cggagtatca gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag
      361 agaagaacat cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa
      421 tcagggtaaa gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc
      481 gttcgacaaa atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca
      541 cggctgccat cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga
      601 ggctgaagat aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc
      661 gtgttttcag cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc
      721 agataaggaa caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga
      781 atattgtaaa aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca
      841 gacgcataac catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg
      901 gaagctttgc caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca
      961 gcagcgtcag cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca
     1021 tctaaaaagc cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca
     1081 aagcgatggc tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt
     1141 caaataaaag ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt
     1201 aagcgctttc gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt
     1261 gatccgctca acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag
     1321 ctgtcggtct tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc
     1381 cagcgggatg ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg
     1441 cacatggctg gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa
     1501 tgttgtttcc agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag
     1561 agcagcaaaa tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa
     1621 cacgccaatc cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc
     1681 cgccagttct cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc
     1741 agtttcggga ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa
     1801 aacaaacagc aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca
     1861 attctctgat ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata
     1921 ggttttccaa atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa
     1981 gctgggataa ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg
     2041 aaactccagt ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg
     2101 gcattggttg atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact
     2161 ctttttcatt actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc
     2221 tatatcgggc ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca
     2281 actctatcca cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact
     2341 ctgcacataa ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt
     2401 ttaccgtctt cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt
     2461 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
     2521 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
     2581 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
     2641 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
     2701 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
     2761 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
     2821 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
     2881 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
     2941 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
     3001 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
     3061 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
     3121 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
     3181 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
     3241 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
     3301 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
     3361 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
     3421 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     3481 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     3541 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     3601 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     3661 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     3721 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     3781 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     3841 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta
     3901 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     3961 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     4021 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     4081 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     4141 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     4201 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     4261 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     4321 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc
     4381 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     4441 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     4501 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     4561 tgcctgggtt ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     4621 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     4681 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     4741 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     4801 ccgtcaatgg tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc
     4861 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac
     4921 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     4981 gaaatacatg gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg
     5041 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     5101 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     5161 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     5221 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     5281 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     5341 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     5401 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     5461 cccgtggcgc ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg
     5521 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     5581 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     5641 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     5701 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     5761 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     5821 ggtgccgggc gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac
     5881 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     5941 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     6001 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     6061 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     6121 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     6181 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     6241 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     6301 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     6361 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     6421 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     6481 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     6541 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     6601 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     6661 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     6721 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     6781 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     6841 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     6901 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     6961 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     7021 gactttgaga ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa
     7081 ttacgaaaca tactgccaga gccgacgcca gtgtgcgtcg gtttttttac cctccgttaa
     7141 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     7201 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     7261 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     7321 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     7381 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     7441 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     7501 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     7561 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     7621 gcatgggccg ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     7681 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     7741 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     7801 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     7861 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     7921 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     7981 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     8041 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     8101 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     8161 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     8221 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     8281 acctgtgaac cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt
     8341 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     8401 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     8461 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     8521 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     8581 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     8641 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     8701 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact
     8761 gcgttatcgg gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa
     8821 cagctctggc ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acctgccaac
     8881 tggcaggtca accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta
     8941 gtttcacccg ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag
     9001 tcaggagccg ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg
     9061 atggagttta ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg
     9121 gtgcaaagta ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt
     9181 gcggcaaaca ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg
     9241 ttaccggaaa tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg
     9301 actctgctgc cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact
     9361 gacgatagcg acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac
     9421 cagtatctgg tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa
     9481 gggctgggag aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc
     9541 catgaactcg gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg
     9601 ctggagtccg cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt
     9661 tccgcgttac cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc
     9721 catctcctgt ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat
     9781 ctggcgaaac tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta
     9841 tttcgcgata gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc
     9901 aacccgctgc tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac
     9961 ctggagagca gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat
    10021 aacattcagt ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa
    10081 gagttttccc gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc
    10141 cacgtttgcc atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg
    10201 ctggaggaag ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac
    10261 agctacagtc cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct
    10321 tacgccattt ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc
    10381 ctgttatcac tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg
    10441 ccggtgctgg cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg
    10501 gtcaacgaat ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc
    10561 cccgccaccg gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg
    10621 atggagagcg cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta
    10681 attgcagaac tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc
    10741 gggctggcac aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac
    10801 gccttcttcc tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg
    10861 caggcgatta tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta
    10921 ttgcgtgatg aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc
    10981 ggaccggtta atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc
    11041 ctgctgggaa tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg
    11101 atgagccaga aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc
    11161 ctggaagcgt taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt
    11221 caggataaca gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg
    11281 caaagtcatt atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta
    11341 aaagcgcatc ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca
    11401 ggcgaacgac aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca
    11461 cattctgaat ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg
    11521 ctacaacgat tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac
    11581 ttccgtactg aagacagcga aatcccagac accgagccat ttattctgga aggacttagc
    11641 cgttatcaaa tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc
    11701 ttgttccgcc gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc
    11761 tgggaaacac agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag
    11821 ccggggcaga gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg
    11881 ccgcaggtgc agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag
    11941 ggaatgcaac tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt
    12001 cgcctttttc tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct
    12061 ttgcattacc tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg
    12121 gtgttacctg aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc
    12181 atgctggatg acgattccac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa
    12241 ggcaacatga tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa
    12301 ttaacaccag agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg
    12361 tttcgcttta atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta
    12421 tgatgcgcac cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa
    12481 gattaaattc tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg
    12541 attatgaggt ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc
    12601 cctctgggca ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg
    12661 taaaagtgat aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt
    12721 cttgctggtt tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg
    12781 gtcgctggaa gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct
    12841 gatggggtcg aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg
    12901 cggtagtcac aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa
    12961 cgacgccttg cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga
    13021 caagaaatac gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac
    13081 gcgtgacggg atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg
    13141 ttcaaagttt tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca
    13201 gcaggcgctg aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat
    13261 ttacagtaat aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt
    13321 gccgaacaaa gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa
    13381 gggcattatc attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg
    13441 catcgataac aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat
    13501 tggcaatcgc agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg
    13561 cattagcgcc aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc
    13621 gtctttaact gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta
    13681 tctcaatctg ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt
    13741 gctggatatc gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct
    13801 ggcagatacc atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga
    13861 tcggtacgat gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg
    13921 tatctggtat atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc
    13981 gtatcaggtc gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga
    14041 cattgcgctc tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa
    14101 gtcagagaag aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt
    14161 gtatgcgcca agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg
    14221 taatccgaaa gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct
    14281 cgcagggctg gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc
    14341 caccaacgct aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca
    14401 gctgttccag gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga
    14461 gcaggcgaag tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga
    14521 gcaggcgatt atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg
    14581 gcgtaaaatt ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa
    14641 atcaggggct cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac
    14701 gctggcacgc gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa
    14761 agatgtagtg gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga
    14821 ctccgcactg gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta
    14881 tagctctctg ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga
    14941 acaattgggc tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg
    15001 cttccttttg caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt
    15061 tttcccaacc gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca
    15121 gcaggcggta attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa
    15181 gttaagtaaa gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc
    15241 ccagataaaa ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga
    15301 gccgcaaggc atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata
    15361 tgtgcatcct gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc
    15421 cctgatgagt gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct
    15481 tacagggcga gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg
    15541 cgctctattt gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga
    15601 tcgttgaaga actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc
    15661 gtatccgtag caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc
    15721 cactgtacga acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt
    15781 tgttagccga acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc
    15841 gcatgctcaa cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag
    15901 atgagtctct gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc
    15961 tgccgcgtga aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc
    16021 gcgatattaa tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg
    16081 atgaaacgct ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc
    16141 agtggcgcga cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac
    16201 gcaagtttaa ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag
    16261 aagagacaaa cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag
    16321 aagatcgcac gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc
    16381 aactgcttgc agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga
    16441 tccgcgaaac agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt
    16501 taagtcggct cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc
    16561 gtacgcgatt cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc
    16621 gaatttttcg ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc
    16681 cgaagcaggc catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta
    16741 gtgaagttca cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga
    16801 acagtgtgaa caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt
    16861 ttattccagt gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa
    16921 cgcagcctgc gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc
    16981 aaagtaccat ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc
    17041 ggggcgaagc gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca
    17101 gcgtgctggt gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg
    17161 aaatcccttc cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg
    17221 aaatgctttg gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg
    17281 cgctggcaac gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg
    17341 aacatgcgtg ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac
    17401 gtggcgttat gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc
    17461 tggcaacggc aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc
    17521 aagaagccgg aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata
    17581 tcctcgagcc agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc
    17641 tggtgcagat tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc
    17701 catttattac caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg
    17761 aggcagtttt ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc
    17821 tggcggaaga tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc
    17881 tcggcgttgc accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc
    17941 aaagtgcgct cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca
    18001 cctgtattga agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata
    18061 accagccctg gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac
    18121 gattgcccgg cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg
    18181 gtatcgctca ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg
    18241 ttgaagaacc gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct
    18301 tgcacagttt gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg
    18361 aaaaactgga actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca
    18421 cggctgtcct ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc
    18481 gcgataaaca ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc
    18541 agcttgatgc actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt
    18601 tcatgcaggt acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc
    18661 gttattacct gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc
    18721 aacaggctat ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata
    18781 ccctggcact gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact
    18841 ttggcggcgt tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga
    18901 tttacacaac ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta
    18961 tgaccctgga ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa
    19021 acagctacgc ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc
    19081 tgtcaccctc gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc
    19141 gctttcacga ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga
    19201 aatcggtgag ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg
    19261 agatgaaccc acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg
    19321 taacgagcgg acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga
    19381 agctctactg gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca
    19441 aaaagttgca gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg
    19501 caccggtaaa acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg
    19561 cgaacgctgc cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga
    19621 atctctcggc aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc
    19681 ggaagatgcc agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg
    19741 tcatcatgcc ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat
    19801 cgatctgcct atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt
    19861 tctcggcgat cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg
    19921 cgcttatgcc aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg
    19981 gactcacgtt ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct
    20041 gctgcaaaaa agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat
    20101 caaccgtggt gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga
    20161 aaaacgactt ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg
    20221 ttacggacgt tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc
    20281 gttcaatgag taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact
    20341 gaatgagcga attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc
    20401 tcgttggtac gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt
    20461 taatggcgat atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat
    20521 gccggacggc aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg
    20581 ggcgatgacg gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc
    20641 gagccaacgc acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg
    20701 tcgtcgtctg tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac
    20761 tgagcggcgc agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga
    20821 tgcgacgcac gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc
    20881 gccatcaaca ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt
    20941 aaatcaatat ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc
    21001 agcacaaaca atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga
    21061 acctcgcccc gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca
    21121 atcttctctt ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga
    21181 ataatggtga atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata
    21241 ccttgctgct ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg
    21301 cgacgaatct gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac
    21361 aactcttgca acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg
    21421 ctgcggcagg ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct
    21481 ttctcttcct gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata
    21541 tcaccgtcgt cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc
    21601 agtttgatgg ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg
    21661 actgaaacag cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc
    21721 gcgtcttcat caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg
    21781 cccagcggct gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc
    21841 ggcgtgttat tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca
    21901 gcctgcttca ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc
    21961 ggttcgtggt gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc
    22021 accagacgga tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc
    22081 tcatgctcaa tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg
    22141 atatagggaa ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg
    22201 gcacacccct ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg
    22261 tgcaagtgta aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa
    22321 gaatgtttac cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa
    22381 ttagtcgttt acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat
    22441 ccaacactgc aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga
    22501 gcgtaagtca ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg
    22561 cgtccagcta cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg
    22621 cgttgagtaa ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac
    22681 tcaaggggat ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg
    22741 ggcaatttga cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc
    22801 agctgtttgc ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc
    22861 cggccaatgc acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag
    22921 gcgacctcga aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc
    22981 gcgatcgtcc gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg
    23041 tggggaaata caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct
    23101 ctctgatcga gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca
    23161 ttccgttgca agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta
    23221 tccatgccga cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa
    23281 ccaaaggcgc aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact
    23341 tgattggtgg cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg
    23401 tgcagtcgct gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg
    23461 gaaaaatcaa caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg
    23521 caccagatat tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc
    23581 gtaaactgaa aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta
    23641 aagcgtattt tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat
    23701 ggcgcttttt ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg
    23761 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    23821 ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt
    23881 ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagctta
    23941 ttttttgtgc gaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg
    24001 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
    24061 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac
    24121 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
    24181 gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat
    24241 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
    24301 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
    24361 gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
    24421 tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa
    24481 acgtggtcgt ctgccgtctc gtgcggagat ctacgccggg gcattgggcg ataaatacat
    24541 cctcgcttac agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta
    24601 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
    24661 tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat
    24721 gtcgatgcag gcgattcgtc attggggcaa aacacacagt gaagccgagg ttcgcgagct
    24781 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
    24841 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
    24901 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
    24961 tcagtacgaa ttgcgtctga tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca
    25021 cttcgatatc tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca
    25081 ctatggacgg gtttgggtgc tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg
    25141 atgtgatatt ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcacatcc
    25201 tgattattga ctaagtgcgc gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc
    25261 tacaaatcat gcaaatccaa aatattgcag gagttatgta ggcctgataa gcgtagcgca
    25321 tcaggcattt atgcgtttag ggtgagggat aacctcgccc tttttaattc tgaggtgcta
    25381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
    25441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
    25501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
    25561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
    25621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
    25681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
    25741 gttattcgta cgtgattgat gccattgata gtgtacggcc caaagcggcg ctgattgctt
    25801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
    25861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggatccgctg gcggcgaagt
    25921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
    25981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
    26041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
    26101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
    26161 tggcgaaagc ggcgcgtcag ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc
    26221 cgacccggtt ctgtgcttaa acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat
    26281 ttaacccctg gctgcgtgag gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg
    26341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
    26401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt
    26461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
    26521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
    26581 atttatcttc ccattgcgac aacggtgcga aggtattgcg taacgtttct gcggttacgg
    26641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
    26701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
    26761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgttg
    26821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
    26881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
    26941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
    27001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga cgccagcgac
    27061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtggtgaaac cgtcaaaact
    27121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
    27181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
    27241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
    27301 aaccatcacc accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
    27361 accgccagtc acgttcgaca tctgacccaa cgccagaatc cgactacggg gagtaatcag
    27421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
    27481 ggctctagtt tgttgggcga ccatcagcca ggggacgagg ttagcgtggt gttctgccac
    27541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
    27601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
    27661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
    27721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
    27781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
    27841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
    27901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
    27961 tatctagata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
    28021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
    28081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
    28141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
    28201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
    28261 tcaaccgtac tgacgtgaaa gagatggtcg aactggaaca gtaattcgcg atcgcaaggt
    28321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
    28381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
    28441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaatacg cattccgata
    28501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
    28561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat
    28621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
    28681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt tttactcgcg cagcagaaga
    28741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
    28801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
    28861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
    28921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
    28981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
    29041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
    29101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
    29161 ttcgccgcta ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca
    29221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
    29281 aaatcatatc aacgttcagc aagggccaat ttttagtcat agcgccatgg tgctgcaagc
    29341 ggctattcac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
    29401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaatg ctttttatct
    29461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
    29521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
    29581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
    29641 tgtggcactg ggcgcttttg gtgcgcatgt gttaagtaaa accatggggg ccgttgagat
    29701 gggctggatc caaaccggtc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
    29761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
    29821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
    29881 gtgggcgttt gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt
    29941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
    30001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
    30061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc
    30121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
    30181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
    30241 ccattgtcgg catgttacag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
    30301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taagttcact gttccgctac
    30361 gcgctgcctt gcgcgatgct ggggtgctgg cgaactatga aacgccgaag cgtccggttg
    30421 tgcatgtatt ctttattgca ccaggatgct gctataccgg ttactcatac agcaacaata
    30481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
    30541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
    30601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
    30661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
    30721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
    30781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
    30841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
    30901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
    30961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
    31021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
    31081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
    31141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat acccgatgcg
    31201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
    31261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
    31321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
    31381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
    31441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
    31501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
    31561 ggctatcacc atcctttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
    31621 cgcataatgg cttttcagcc tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg
    31681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
    31741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
    31801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
    31861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
    31921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
    31981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
    32041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
    32101 accctctacc gccgccatca tcaccagcgg cggggcataa ctgtccagct caaataacgg
    32161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
    32221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
    32281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
    32341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
    32401 gtgcacgggc ttcttccggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
    32461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
    32521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcatagg
    32581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc
    32641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc
    32701 cagcgttctg acacgacaat aaatcacact gcattttcac accatccgcg ccaggtgcga
    32761 tcagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc
    32821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccatgcct
    32881 ggctatctag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctagg
    32941 cgctgcgaac cattaaaatt tcccatgtac cggaggagag cacgggttca ttttgttcgg
    33001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
    33061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
    33121 ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga
    33181 aatcgcgttg ctggatatcc agcatctggc tggttccggc tatcgtgata tcagtagtga
    33241 attcgccggt taaacggtgg ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt
    33301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
    33361 aggctccgac gccagaaata gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt
    33421 ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
    33481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
    33541 attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcag
    33601 tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt
    33661 gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg
    33721 ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat
    33781 aacattgttt atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg
    33841 gcatcacatc agggtgtaat gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg
    33901 cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg acgatacact
    33961 ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc aagggtgata
    34021 aagtctgcgc caacgtggcc gatggtcaga actccgtggt tggcacccca gttcgccatt
    34081 accgaataca catccgtaaa cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt
    34141 ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg
    34201 cttcagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc
    34261 atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag
    34321 tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga
    34381 gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa
    34441 ccgtccagcg cagcagaacc tgagttgatc aaatggatga tgccgtgttc tgccagtccg
    34501 tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg
    34561 gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac gccgttaaga
    34621 ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc aaatgaactg
    34681 ttgaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg acgttgcccc
    34741 tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc aatatcggcc
    34801 agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc
    34861 agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca
    34921 tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc gtcgtaaatc
    34981 ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc
    35041 caggattcaa agaagttgtg atcaacaata gaaccggcga tacccatcga aacgccgccc
    35101 agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc
    35161 agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga
    35221 ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg
    35281 taaacagcgc cggggcgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga
    35341 tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct
    35401 acattctggc tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag
    35461 gtatcggaaa tgacacactc gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc
    35521 agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc
    35581 atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt
    35641 acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga gaacggaaac
    35701 gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt
    35761 tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg ccaataatgg
    35821 tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg
    35881 agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc
    35941 ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg
    36001 cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa
    36061 taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca gtcatacctg
    36121 gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt gcgccgacat
    36181 agcagaattg cgctaatacc gcccagcgcc agacgagaaa gcgatgcgga gaacgatcct
    36241 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    36301 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatact
    36361 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    36421 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
    36481 aacgagttaa atgtttgcgc aagatttaag cggaagtgac tactgctttc cggccctaat
    36541 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    36601 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    36661 cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc
    36721 aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    36781 ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
    36841 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    36901 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    36961 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    37021 aaaagtgcaa aaccagcgag ttcgggcttc atatcggtga atatttgcct gtctgaaatg
    37081 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    37141 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    37201 ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt ttgtgccgaa
    37261 aacgggaatt tctggcgagg ggaaatggaa aaatgaccgt tttgataaat atttttaaga
    37321 gcggtcattt ttaacggggt tataa
//
LOCUS       DADLTI010000017        36408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLTI010000017 DADLTI010000000
VERSION     DADLTI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.017"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(24..278)
                     /locus_tag="JN755_000822"
     CDS             complement(24..278)
                     /locus_tag="JN755_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7379711.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JN755_000823"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JN755_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7379712.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JN755_000824"
     CDS             1228..2694
                     /locus_tag="JN755_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7379713.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JN755_000825"
     CDS             2812..3798
                     /locus_tag="JN755_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7379714.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JN755_000826"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JN755_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7379715.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JN755_000827"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JN755_000827"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7379716.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JN755_000828"
     CDS             5709..7265
                     /locus_tag="JN755_000828"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7379717.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSRTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JN755_000829"
     CDS             7347..9161
                     /locus_tag="JN755_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7379718.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JN755_000830"
     CDS             9162..10256
                     /locus_tag="JN755_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7379719.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JN755_000831"
     CDS             10256..11281
                     /locus_tag="JN755_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7379720.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JN755_000832"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JN755_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7379721.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JN755_000833"
     CDS             complement(12876..13220)
                     /locus_tag="JN755_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379722.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN755_000834"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN755_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7379723.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN755_000835"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN755_000835"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7379724.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JN755_000836"
     CDS             15616..17376
                     /locus_tag="JN755_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7379725.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JN755_000837"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JN755_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7379726.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN755_000838"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN755_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7379727.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JN755_000839"
     CDS             19113..19340
                     /locus_tag="JN755_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7379728.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JN755_000840"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JN755_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7379729.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JN755_000841"
     tRNA            21195..21271
                     /locus_tag="JN755_000841"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN755_000842"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN755_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7379730.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JN755_000843"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JN755_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7379731.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JN755_000844"
     CDS             complement(25143..26195)
                     /locus_tag="JN755_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7379732.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN755_000845"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN755_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7379733.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JN755_000846"
     CDS             complement(26746..28689)
                     /locus_tag="JN755_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7379734.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN755_000847"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN755_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7379735.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN755_000848"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN755_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7379736.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN755_000849"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN755_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7379737.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN755_000850"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN755_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7379738.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN755_000851"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN755_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7379739.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN755_000852"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN755_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7379740.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN755_000853"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN755_000853"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7379741.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN755_000854"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN755_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7379742.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN755_000855"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN755_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7379743.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..>36408)
                     /gene="napA"
                     /locus_tag="JN755_000856"
     CDS             complement(34052..>36408)
                     /gene="napA"
                     /locus_tag="JN755_000856"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7379744.1"
                     /translation="PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLP
                     KIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMF
                     GSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIE
                     QADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQ
                     SDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA
                     SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHT
                     RGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNE
                     KHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEER
                     MPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAP
                     GEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELA
                     EDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQW
                     RYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWH
                     TGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNR
                     PPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
BASE COUNT         8869 a         9858 c         8989 g         8692 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtagaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatgct gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcgcttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcggt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atagcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagtgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacaaacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcg
//
LOCUS       DADLTI010000018        36241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLTI010000018 DADLTI010000000
VERSION     DADLTI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.018"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..249)
                     /locus_tag="JN755_000857"
     CDS             complement(<1..249)
                     /locus_tag="JN755_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAY7379745.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDT"
     gene            complement(262..921)
                     /gene="eutL"
                     /locus_tag="JN755_000858"
     CDS             complement(262..921)
                     /gene="eutL"
                     /locus_tag="JN755_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7379746.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(931..1818)
                     /locus_tag="JN755_000859"
     CDS             complement(931..1818)
                     /locus_tag="JN755_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7379747.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(1839..3200)
                     /locus_tag="JN755_000860"
     CDS             complement(1839..3200)
                     /locus_tag="JN755_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7379748.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            3389..4373
                     /locus_tag="JN755_000861"
                     /pseudo
     CDS             3389..4373
                     /locus_tag="JN755_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4857..5597
                     /locus_tag="JN755_000862"
     CDS             4857..5597
                     /locus_tag="JN755_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7379749.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQN
                     KAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSK
                     EEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCR
                     YRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLR
                     IPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            6648..7400
                     /locus_tag="JN755_000863"
     CDS             6648..7400
                     /locus_tag="JN755_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379750.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            8160..8555
                     /locus_tag="JN755_000864"
     CDS             8160..8555
                     /locus_tag="JN755_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7379751.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            8573..8836
                     /locus_tag="JN755_000865"
     CDS             8573..8836
                     /locus_tag="JN755_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379752.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            8859..9401
                     /locus_tag="JN755_000866"
     CDS             8859..9401
                     /locus_tag="JN755_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379753.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            9866..10072
                     /locus_tag="JN755_000867"
     CDS             9866..10072
                     /locus_tag="JN755_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379754.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            10531..10869
                     /locus_tag="JN755_000868"
     CDS             10531..10869
                     /locus_tag="JN755_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7379755.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            10850..11128
                     /locus_tag="JN755_000869"
     CDS             10850..11128
                     /locus_tag="JN755_000869"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379756.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            11121..12581
                     /locus_tag="JN755_000870"
     CDS             11121..12581
                     /locus_tag="JN755_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7379757.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            12603..13424
                     /gene="murI"
                     /locus_tag="JN755_000871"
     CDS             12603..13424
                     /gene="murI"
                     /locus_tag="JN755_000871"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7379758.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(13543..14234)
                     /locus_tag="JN755_000872"
     CDS             complement(join(13543..13988,13988..14234))
                     /locus_tag="JN755_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7379759.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(14758..16131)
                     /gene="eutA"
                     /locus_tag="JN755_000873"
     CDS             complement(14758..16131)
                     /gene="eutA"
                     /locus_tag="JN755_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7379760.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(16106..16585)
                     /gene="eutP"
                     /locus_tag="JN755_000874"
     CDS             complement(16106..16585)
                     /gene="eutP"
                     /locus_tag="JN755_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7379761.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(16598..16933)
                     /gene="eutS"
                     /locus_tag="JN755_000875"
     CDS             complement(16598..16933)
                     /gene="eutS"
                     /locus_tag="JN755_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7379762.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(17226..19505)
                     /gene="maeB"
                     /locus_tag="JN755_000876"
     CDS             complement(17226..19505)
                     /gene="maeB"
                     /locus_tag="JN755_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7379763.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            19794..20744
                     /gene="tal"
                     /locus_tag="JN755_000877"
     CDS             19794..20744
                     /gene="tal"
                     /locus_tag="JN755_000877"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7379764.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            20764..22767
                     /gene="tkt"
                     /locus_tag="JN755_000878"
     CDS             20764..22767
                     /gene="tkt"
                     /locus_tag="JN755_000878"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7379765.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(22881..23912)
                     /locus_tag="JN755_000879"
     CDS             complement(22881..23912)
                     /locus_tag="JN755_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7379766.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(24038..24613)
                     /gene="nudK"
                     /locus_tag="JN755_000880"
     CDS             complement(24038..24613)
                     /gene="nudK"
                     /locus_tag="JN755_000880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7379767.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(24681..26660)
                     /gene="aegA"
                     /locus_tag="JN755_000881"
     CDS             complement(24681..26660)
                     /gene="aegA"
                     /locus_tag="JN755_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7379768.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            26866..28589
                     /gene="narQ"
                     /locus_tag="JN755_000882"
                     /pseudo
     CDS             26866..28589
                     /gene="narQ"
                     /locus_tag="JN755_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            28791..31904
                     /gene="acrD"
                     /locus_tag="JN755_000883"
     CDS             28791..31904
                     /gene="acrD"
                     /locus_tag="JN755_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7379769.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(32003..32062)
                     /gene="ypfM"
                     /locus_tag="JN755_000884"
     CDS             complement(32003..32062)
                     /gene="ypfM"
                     /locus_tag="JN755_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7379770.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            32443..32799
                     /locus_tag="JN755_000885"
     CDS             32443..32799
                     /locus_tag="JN755_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7379771.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            32803..33930
                     /gene="dapE"
                     /locus_tag="JN755_000886"
     CDS             32803..33930
                     /gene="dapE"
                     /locus_tag="JN755_000886"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7379772.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            33958..34158
                     /locus_tag="JN755_000887"
     CDS             33958..34158
                     /locus_tag="JN755_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7379773.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(34268..34966)
                     /gene="ypfH"
                     /locus_tag="JN755_000888"
     CDS             complement(34268..34966)
                     /gene="ypfH"
                     /locus_tag="JN755_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7379774.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(35040..>36241)
                     /locus_tag="JN755_000889"
     CDS             complement(35040..>36241)
                     /locus_tag="JN755_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7379775.1"
                     /translation="HQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQ
                     PIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLS
                     GAHYRTSPLDLRRMMDALGQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRP
                     RGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYL
                     SVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRL
                     RRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSE
                     LALPALRGCLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRI
                     TQWQFFH"
BASE COUNT         9232 a         9166 c         8981 g         8862 t
ORIGIN      
        1 ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc
       61 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
      121 cgttaagcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
      181 catggcatcc gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
      241 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
      301 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
      361 tgccgagtag ttggtttcag acggtggcgg gacgtaggtc accagctgta cgtcggcaga
      421 tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
      481 cggatcgcca agcgtgatcc cggcggttga tgaaagataa gaacctgtac gcgaaactac
      541 atgcgccagg aaggcggtgt tttctgcgtc attggcccac tggaaagctg cgccattttc
      601 aatatgtgca accatcgcat ccagaccggc acgcacttcc gccgggtttg gcccaccaag
      661 cataatcagc acttcaccgg cagtcggtga cgggccgtgt gccgctcccg catacagcga
      721 gcgaccatat accacttcga ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt
      781 tacgtcgtca gaatcggcag aaatcagccc gaggctacga atatgcggcg gcaattttag
      841 ttcacgcgca aattcagcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg
      901 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
      961 ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt
     1021 gccgccctgg tgaatgttag agatacaggt gcgatcggcc tcaacggtgg tcgccatacg
     1081 cggcgagtaa acggcgtagc aggagaggct ttctgactgc cctaaccccg gacgttcccc
     1141 caccagcagg atcaccactt tcgcgccgag gatctcaccg atctgatctt caatcttcac
     1201 gcgaccataa cgaacaaaga atggcgtgcc gactttcagc ccggcctgtt tcaggcccgc
     1261 catcagcggc ggcaggatct cttcgtagtt gacggtgatg gcatcagttg acaggccatc
     1321 agaaataacg acctgtacgt ccggattagc aacacactgt gctttcagcg cttcaacagc
     1381 ttctgcacac aggcggcggc ccatatccgg gcgagtcagg tagagatttt tgtcgctaat
     1441 ttcagagcgt acttccagca agccctgcgc tttcacccac tcttccggca cttctttcag
     1501 tacggtatct ttcgaacgag agtgatcggc caagaaacgc agcagcgcct gagtacgtgg
     1561 acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcggcgca gttctgttaa
     1621 tacgtctgcg cgatgcgggt tttcaacacc aatccacgct tttgcttcag cggaacctaa
     1681 atccagcgca cagctttccg aggtcaccgg tgccgcacag ttggtggtgg cgcactttgc
     1741 ttctgacggc gcgggggccg cttgtcccat tgacgccatc acgctgcgta caatttcttc
     1801 aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg
     1861 cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca
     1921 aactctggtg acgggcgcag gttgagtaac tgacgcacag tggcggtatc gtggaatgcg
     1981 gtggtctggt agttgagcat gatgtcatca cccagcggca tccccatgat gtagttgcag
     2041 cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcaga gtcagcgtgg
     2101 ttggtgtagc agccgtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct
     2161 tctaagccag cacggataat ctggcggtcg ttgtagagat actccggccc aataaagccg
     2221 accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgtgcttcc
     2281 atcgtcacct ggtcagcgcc gaagttagcg ccagcggata gcgcagagcc ttgtccggtt
     2341 tcgaagtaga ggcagttttc cccggcgata cgattgaact ctgcgcccac tgcgcgcgct
     2401 tcgtcgagca tcgccagttc cacgccaaac tctttcagcc ctttttcgct gccacagata
     2461 ctctggaaaa tcagtccgcc aggagcgccg cgacgaatcg cttcgatctg ggtggtgacg
     2521 tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc ataaatggta
     2581 tccagcacgc ggcttaagtt ttccacgtcg tcagttaccg ggttaacgcc gatcaccgca
     2641 tcgcccaccc cgaaggaaag cccttcatag atttgcgcag cgatactttg cacgtcgtca
     2701 cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg gaataccgat ggtggtattg
     2761 gcctttttga ttaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc
     2821 ttcgctatcg ccgcgaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc
     2881 acgctggttt catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc
     2941 tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc gcgtcacgca gtcatcttca
     3001 taggcaatca ccggattatt gcggatgtcc gctacggtca tttccgacaa cacctgcttt
     3061 gccgccacgc gctcctgtga gcttgccgct gcaacgcccg ccagcacatc ccccgaacgc
     3121 agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac attgccgaac
     3181 aatgtggtct ttagtttcat aagtcgttcc ctcaggaaga agtgatgagt aacatttctc
     3241 acagcctgca aacaattcta tttcatcgtt agcccttgtc atgtaaggtg atttccttaa
     3301 cacgcccgtt acttatcctg ccgaacgcaa ccgaactcgc cggatacatg agcggggtta
     3361 tcgagtcgtt agatcgaggt atccggtttt gagcaaaaca ccactcacag caaaggccat
     3421 agacgccgca caaccaaagg acaagcaata caaactcacc gcttcactga cgcccggcct
     3481 gtttttgctg gtgcatccca acggcagcaa atactggcga ttccggtact ggatagacaa
     3541 gaaagagcgg ttacaggcta ttggggtata tccgctgatt agcctcaagc aagcgttcaa
     3601 tcgttgatat aaccgcccgt gacgtgctgg cattactcca gaagaaagaa cgtaccgcac
     3661 cggagcaggc ccgcaagcta cgccagcgca tcggtgagat cttcaagttt gccgttatca
     3721 ccgaactggt aacgaggaat ccggttgcag atcttgatac ggcactgaag gcccgccgac
     3781 ctggtcacaa cgcgtggata ccgattagtg aaattccggc cttctacaaa gcccttgaga
     3841 gggccgggag cgtccagatt caggcggcaa tacgtttgct tatattaacg gcattaagga
     3901 ccgcagaact gcgtttgtgc cgttgggaat ggataaacct ggaggacgca acaatcacat
     3961 tgcccgctga agtcatgaag gcccgccgcc cgcatgtggt cccgctatca cggcaggcgg
     4021 tcgagctatt gcaggaccag ttcacacgca gcggatacag tgttttcgtg ttcccgggcc
     4081 gattcatgga taagccatta tcagccagcg cgatactcaa ggcgcttgag cgtatcggat
     4141 acaagtcaat agccacaggc cacggctggc gcacaacgtt cagcaccgca cttaacgaaa
     4201 gcggcaggta taaccctgat tggattgaga tccaactagc ccacgtcccg aagggaatta
     4261 ggggagttta caaccaagcg gcctatctca aaatgcgccg tcaaatgctt caggactacg
     4321 ccgacgcgat tgattcgata ctggctgatg gtatcaatcc gcttgaactg taatgaatga
     4381 ttaatcacgt tttccgacga ctccaccgga gtaggccgga taaacactgg acaccttcaa
     4441 aataactatt cactttttcc ggattcgtcc ggcttttttt gtctttgccg atgggatcaa
     4501 ttattccaat tattccagta tcgttggaat aaatgaataa atatcaatga cttaaagccg
     4561 tgtttgcaac agggtggatc ggggatcgaa aataggatca acacaaaaac accctggagg
     4621 ctacagcaca ctagggggac acaaaaacaa cacaggaaaa aaataccatg taccaatcag
     4681 caaccgccta cgtggcgaaa ccaggcgaaa cactggtcaa acacaaaatt gcaacactcc
     4741 aagcacttca aacactccaa gcccttccgg aacaggttca ggccgccttt cgtgatggca
     4801 tgaacatcat ccgcttcgat gggtatcgct gggaatacaa gccagcaact ggattcgtgc
     4861 gcatagatca aatcttgtcg ccagccaatg aagagccagc gctagccaat gcagaaccag
     4921 cgcaggagca ggagcaggag caggagcagg agcaggagca ggaacaggaa caggaacagg
     4981 aacagaataa agcggcggct attgccgagt tcaaggagct gcaaagacaa caaatcacag
     5041 cgcagcaaga agaatgccgc cacccgctgg caatgcccaa gccagtagcg aaagccaaca
     5101 cagacaagcc gacttcatgg tttaacgaag aagcggaaga agatttgcgt tttgatagta
     5161 aagaggagca gttgcctgaa gagcgggaac aggtggaaac ggaagaccag caaaacgatt
     5221 atcgcaacct tccagccgtc aataacgcta tggcttacct cttcaaaagc gggctgtttc
     5281 acaagctgaa aggcgatgag ttaaagctat atacgtggat ggtatccaag tgcaggtatc
     5341 gcggtaacga caaatatgag atttggtatt cccgcaccaa aatggcaaaa gcgttaggta
     5401 agagcgttga cacgatagat cgccacctgg aagcgttgag aaagctggga cttatccagg
     5461 aaatgcgcca ggcactgagg ggcaaatgtg gaaaaaccta cagactgcga atcccgtcaa
     5521 aaggcacagc agaagaggcc tcaatgacgg cacagcagca cgaaagagaa gcaaaagtca
     5581 gctacctggt acactaggca gcgggtcagc aaaggggggc aaatgcccct tttttcatgt
     5641 cagataatca gatcattacc tcaataaact aataatcaca tcgcggttac aaccagtttg
     5701 atagtgatag ctcaaaccca gacgtggcgc tggctgcaag caaaataagc cgcaaaaatg
     5761 cggtcctatt tgccagaacc ttttattatt caattggtta gtgcaagaaa aagcgtgatt
     5821 actgttcaag aagcatccag aaaggtcatg attttgccat gattttgacg ctgaaaaaaa
     5881 ataggtgggc atttttgccc actcactagc cgcaaatttg cccactcact agccgcattt
     5941 ttgcggccca aagtaataaa tacagagaaa taataaatac agatcagcgc caagattttt
     6001 cgtcttttcg gcggaatcat gagactagtg cttacgtcct gataacctcc agagcagacc
     6061 ttaacttctg tgcagcagga ctacgctcat tgccgctcgg ctaggtcaaa tcctcacgat
     6121 aaagctgttc ggattgtgac cataatgtta aaggactctt taaatcattc cttgacgaat
     6181 taactagctg gcttaagcgg aatagcatgc aggataaaaa caaaaccagc ttagcagtgg
     6241 ggagagcttc gcttcgctcg tctcctgggg ccaatgctca atgaaacgac gtaaggcacg
     6301 gagcgctcac cataggtaga cacataaaca tctgagagcg caccttctat cgcgctacgc
     6361 gctcaataaa aaccaacgac accgccacac tgcaagacca atggttttta tcgcgtatac
     6421 agaccagaca ggaaacagca gcaaagcagc gtatagccag gtgcagacag cacactcaca
     6481 cgacgcacag caggcttaag tgatcactca ggaaacttta tccccgccgc cgatcaccgc
     6541 gaactatcat gaccgcgcca aattcgaaag gaatggccca tagaacgcga tgaacacaca
     6601 taagcgctaa cttgcttacc tgaaaactta acgtcgccac agagcgattg agagcgctta
     6661 aaatcgatgc gatttcatta aatttttctc actcgcctga atggtggcga tcaggactga
     6721 tcgcaacatt gccccgcctc gatattgagc gcataataga tacatacatt aagcagaaaa
     6781 gagataagtt tattgtgaaa atcagggggt tacacatcat gcgcatatca gaacaacaac
     6841 ggcagatcct cgactatctg gcaaaaaacg aatcaatcac cgcacgacaa gccgcagatc
     6901 tcgtttatgg tggcaatgtt acccgcgtcc aggttgacgc cgcccgccgc tcactcatga
     6961 cgatggttag taatgggcta atcaggaaac aaggccgcgc tttcgtagct gaaagacata
     7021 tcaatcacgc ttatagccag gacgttgaag aattgcgccg ccagatcctt tgggtattca
     7081 gcaaacaggg tcaggagtat ttcctttctg aacacccaaa tttcgccgcc aaagagttaa
     7141 cagtaagcgg attgtcgtgc gcactggatt atcttgtcgg tgaaggttgc gttacttaca
     7201 agcagctatg gcacgccctg aatacgcttg tagaagatgg tttactcatt gccacgcgtg
     7261 agaacatcga atactggata atgccgcgtt caggtcgccg caatcatgca cggtatattt
     7321 caggcaacag atacagaacg ccggacttta atccgacaca ggaagatcgg gaccgtgccg
     7381 aaatgatgtg cagggaatag gagaaaagaa ttatggataa gttattttcg atgcagtgaa
     7441 cacaagcggg cgcaatgccc gtttttattg ccttattcca cgcctgacga atcaatttga
     7501 tcacacctga gcaaatgcat agccagaagt taaggccgtt atattgcgat tgggagcgtt
     7561 ttaaacggta tcaatttaat taattctgat gtggtgattt gcaggcaaaa aaaacccgcc
     7621 ggagaagcgg gaaatagaga catatcgttg tagttattaa cagatcactt tgaggtcgag
     7681 ggcggctaac cctcgctaat attatcccgc cattaccttg cgggtgcaaa taatctttta
     7741 ttgctttgat gatttaataa atagcacttt ttgctaacaa aaaccgctgt caatatatgg
     7801 ccaaaaatgt caacagaaaa aataaggttt ataatattaa gtatcacatg ggtaatgggt
     7861 tcagggaatc ggaatccacc gcctcacgga aattcaaaat ttgcttcttc tttcctccgt
     7921 ggggcggctt ctttttcgta tcgaggtatt tatgaaagca ataacattag aacagttcgc
     7981 agcaatggcg aatgtatcga ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg
     8041 agtccctttg ccatttaccc cacgcggtac tcgccaatta ttctgggagc acgaatgcaa
     8101 agaatggttg tacagaacag aacgataata atcacgcatc acattttgag gtaaacatca
     8161 tgaccaatat cgaaagatta gaacgctgtg ccgatattat gcggcgcaga tggatttatg
     8221 accctgaagc gggtttattg tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga
     8281 ccaataaagg gcatttggtt gtaacggtac atgaaggtaa attcaaagtg acactcacct
     8341 atcagaaagc ctgctatgta tacacatacg gcgcttatga cgagacatta tatgaagtgc
     8401 accacgttaa tttggacaaa caggataacc gcatcaagaa tattcgcctg atgccgaaag
     8461 aaaagcaccg ccggatacat aaaaacgtcc gcaaacttat tcggctgggc cacacacaat
     8521 ggcttcaacc gcaattaatt gaacaatcac tataaacaca gtagtaaata ccatgcattc
     8581 attagacagt tattttcagc gcacgacagc ccctaaatca gccgcccagg aacgccgtga
     8641 ggaattccat gagaaagtga tgcgttctgc tgattatatt gcggacaagt tcgtggaaac
     8701 ggttcgtcca cttgttgatg aagtagctga taaacttcag tctgaaatgc cggaagatat
     8761 ggaaggcact gcaaagcggc gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat
     8821 aagcgctttt aaataatcga aaaaaggggg gaaagataat ggacgcattt gaggtactgg
     8881 caaataaagg cgctgaactg gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg
     8941 cactgctaaa taaacagatt aaagcgatcc gtatggccca gtttaaatta atcagtaatg
     9001 aagttatcga aacaattaaa ccgcagatcc cacaaatcgt tgctgacgct attaaagccg
     9061 ccggactgga gaaacgcatt gccgggctaa aaacgaacgc agagaaaatg gagatgcaca
     9121 aacagagcgg tgctccgaat cccgatgagt acttctcacc ggatgttgag tttatggcgc
     9181 aggttgagga acgtctgggt agctgtctca ccgaggaaca gcgtcgctat tttgatggag
     9241 tggatagttc ggcaggtatt gacctgaact cgtatttcgg tcgtgaaatt gagcattttg
     9301 atgccgcccc gtctatgccg gagccagaac cagaaatggc caccgatcct gaagctgagc
     9361 aacggaaacg cgtattcaat gcgataggga ttcggtactg atcggataaa agaaagccgg
     9421 gaattacccc ggctgactta acaatgtgac caatgaacaa atcggacaag acaaacagga
     9481 taagcagtct cgaaacaatg ttcgggaact atattagcac aagtccggag aaaatcatca
     9541 tgttggcact acgcatcatg cagggcatcg ctaagaccct cgcagaacat gtcttagacc
     9601 tgaaacattc gccactgagc aaacaggcca tgaagcgcta aacgcttcgt ctgtgggctg
     9661 aatattcgct tggaactatc aataaaatca tcgacatgaa atcggggccg agcaaccaga
     9721 gcgcggagga aatggagttc atcaagcgtt taattctcat tcgcagagac attcatagcc
     9781 agttacacag cgtgggaatc gatattaatg atgggacggg cgattaattt tcggacaagt
     9841 actatgcgcg cgcgaggagc tgaacatgaa gcaagatatt gcagacaggc tggagatact
     9901 cgaaggccag cgggcagaag cgaaacaact acgtaagcag gccagaagag cgcacaggaa
     9961 caacgaagca gagttactaa ccaaatacat cagtttcacc aattactgca tctacgagtg
    10021 ttacaaggaa gatgcagagg actggctgga ttcattgcca gaacagtact gagcagaata
    10081 acaccacgtc atgttgttct gaggtgagca tatcacctct gttcaggtgg ccaaatttag
    10141 tgtgccactt cagcatcacc gttgaaatca caataacgat ttttttgtaa atatgctctc
    10201 acatatgtag gaatagacca ggttggcatt gaaacacatc taccatgacc ataataaagt
    10261 aattttttga tatgtttatg gttttatatt aattaaaata tatctaaaat tgatagtttt
    10321 aaaattgcaa aatccagatt gtgtttgtaa tgtttaatct gattgtattg agtggcgttt
    10381 attatgaaaa ataaatcagg taattcttgt tatagagaag cagtcgatat tcatagctca
    10441 cttgagcttt catttatatt gcataaatat catttgcgtg tagatgagaa gaaatctcga
    10501 aatgtattca gcgccatacg tgatgagctc atccttgatg gggattcacg acaaaactta
    10561 gctactttct gtcaaacatg gcttgatgat gaaatccgtg agttgcttaa tttagctatt
    10621 gataaaaaca tgatttataa agatgaatat ccacaaatgg cggaacctga aggtcgctgt
    10681 gtgcgtatgc tttccgattc gtggagtttc cccgattcgc gacacacctt aggttgctca
    10741 accattggtt catctgaagc tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt
    10801 aaaaaacgcg aagtacaagg taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg
    10861 ctggcataaa ttctgccgtt actgtgatgt gaaattgtgt gaaattccac ttgatgatca
    10921 tcacctgata atgaatgcta aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt
    10981 cgttacgact ttaggtatta cttatttttg taaagcagat tgcgttagtg tttttaagat
    11041 aagtgttaaa gttaatatta gtttttatat ttacatcgta attatatgtt tctattttaa
    11101 aaatattcga gagtataaaa atgtctaatg ctaatatcgc acccaaaagt aagaaaatga
    11161 cagtggggac actcgctatc atgaatataa ctgctgtagt aagtctacga tttatacctt
    11221 ctgaagcaga atacggctta ggtgccattt tttactatac ctttgctgca attatgtttt
    11281 tagtacctgt tgcctttgtt gcggctgagc tagctacaac atatccacaa aaaggggggg
    11341 catttcgatg gataggagaa gcatttgggg ctcgctgggg ttttgtagga atgcttatga
    11401 cctggctaca agtcatcccc tactttccaa cagtactgac atttggtgca gtctctgttg
    11461 catttattga tgttgatatg ggggtggcag aatcaattgc cagtaataaa tggtatattt
    11521 tatttttcgt actatttgtt tactggggag ctatttgtgt tgcattacgt ggtgttggta
    11581 ttttctcgca ggtatctaag tggtgcggta ttatagggac aattattcca gctattatcc
    11641 ttgttattct tggtttctcc tatctgtggt tcagcggtaa accagcggtg atcccattag
    11701 gttggggtga tcttttccct aatatagcta acttccagaa tattgttctt gccgccagca
    11761 tatttcttgc ttatgcaggg atggaaatga atgcggtcca tgttaacgat ttagataatc
    11821 caactaaaaa atacccaatt gcaattacca tcgcttctat aggtactatt gcaatattcc
    11881 tgctcagtac tttaggggtc gcctttatta ttcctacaga taaaattagt ctaactcaaa
    11941 gtttgttggt tgcctacgat atgctgttcg aatgggctgg agtgccatgg cttggctctg
    12001 tgatggcatt tatgctagca attggcgtac tgggtggggt ggttacttgg attgcagggc
    12061 ctaatacagg tgtattagcg atcgcgaaag ctggttattt acctaagttt ttccaaaaaa
    12121 ccaatcgtca tggaatgggg caccatctaa tgtttgtaca aggtataatt gtctcagttc
    12181 tttctgttac ttttgtgata atgccttcag ttcaagcagc ttttcagatt ttatcacagt
    12241 taacagttat gctctattta gttatgtata tgctcatgtt cgctagtgct atctatctac
    12301 gacattctca acctgataca aaacgcccat atcgagcccc tgctttattt ttctgggcaa
    12361 cacttggatt cttagggtct tttctggctt ttgtattaag ttttattccg cccgagcaaa
    12421 tcccaactgg tagtccaacc agctatgttc tttatttgtt agtattagtt gccctatttt
    12481 tattaattcc aatattaatt tacgccttcc gaaaatctga ctggaaagat ccaaactctg
    12541 atttcgaacc attttcttgg gaaaaaagaa ctagtaatta aattttccat aagctacaaa
    12601 agatgaggtt aaatatgttt agtgctgaat ctccaattgg tgtaatagac tcaggtgtag
    12661 gtggattgac atcggtaaag gaaataatta atttgcttcc tggtgaagat attatctatt
    12721 gtggagataa tatgaatgca ccttatggca accgttctgc cgatgatatc atcagtttga
    12781 ctaaaaaaat gctgactttt ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta
    12841 atactatatc ctcaactttg gaatccgaaa aatacagcgg atatgctaag agctttccgt
    12901 ttcctatctt atccatcatt gaacctgcag tagaagatgt gattcgccag caatataaaa
    12961 atgttggtat tatcgctacc gagtttacta ttaaaacggg gtgtcacaaa gagctaataa
    13021 aaaaattgaa ttcaacaata aatgtatttg gagaaccaag caaaaatctt gcaatgttaa
    13081 ttgaggaagg taatcttaat gctcctgcaa tcttgaatga catcaaaaaa catgttaacc
    13141 atttaatttc cctccatcca gtaaatgaaa ttattctcgg ctgtactcac tatccaattg
    13201 ttcagaattt ttttgaagca gttgcaccgg atataaaatt tattaatcct gcacatgatc
    13261 aggctatctc tattaaaaat cacttgggac agctaaattt attaaactcc tcaaatatag
    13321 gaactttgca aataaatact agtggaagta tggagatcta caaaacggta ctaggggaat
    13381 tgagcattac gaaacctcat acattcagta tcaggcagtt ttaaatttat ccggtgcacc
    13441 gtaaaatccc gtccttctga actgcacccc aaaagttgga caccaactga gtaaggtgca
    13501 ttttatggac aaacccagtt acatactggg tagtgcatcc aattagtaga acatgtgttt
    13561 ttcgataaac gctccgatca ctttttcgtg gatctcaact gagcgtgaga agcagattgt
    13621 tttacgaccc aaccgcttaa tgcgggtgcg tagcgtcaga ttattacgct caatgcgttg
    13681 ggtgaatatt ttgccggtca gatgcttatt cttcggcacc tcccggccat agctgcccca
    13741 gtcatcgctg gtcagcatgc cgatgttgaa gggtggcagt gccagtagct cccggcacgt
    13801 ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg cctgttttgg tgttgtacgc
    13861 gtacccgagc caatgttgcc gggctttact gccaacgtag ctccattgct catcaagctc
    13921 gcagataaac gccacatcag catgggcaac aggcgaagaa gttattcgct ttggcgcgag
    13981 ttttttaaag tccggatgac ggtgttacta ccaattttca gtgtcctggc ggtatcgcga
    14041 accccggcac cattaaaggc catttcagtt atcagctctt tcatacccgg cttacgtgct
    14101 tgataagtat aagtgaactg aaacacacgg tggcagtcac ggcagcgaag tctgtcacgg
    14161 cctttagggt tctgaccatg gcggtaaacc tgagctgact gacaacgggg acaatgaata
    14221 ttaacgctgg tcatgagata acctcaaaag cccgtattat acatcagatt caactaatta
    14281 gaggcatcac ccatccagca ttataaaaca tactaacaat aactaaatta taataatcac
    14341 aacttacaat caaataataa atgcataaga ttaacaatga ttttttcggg ttatatttat
    14401 ctaaaaaagc agaatattat gtaacttatt attttctaag cgaaaattta ttgtgcagtc
    14461 agactagcaa acttgcgtac cacccgaaga acatgcgaag atttaagtat tctttctttt
    14521 ttggttttta tttttcattt tctcttttct tctctttccg gttctcttct gttctggttt
    14581 tggttttttc ctttttattt ttattctttc ttcagggtta tgctaaaaca ccaaaacatt
    14641 tttcaccaca aaagcatcca atcatcttgc cagaccaatc accttaaccg gatacactgc
    14701 cggatataat cccactaaca cccagccacc aagcaccata cctgataact accgtattca
    14761 ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt
    14821 accaatgtcg atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag
    14881 ttgttgtagc tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac
    14941 caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac
    15001 cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacacgtc
    15061 agttttggga tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt
    15121 ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctccagcca
    15181 gattgtgctg ccagagagcg aaagggtatg tgcacccgcg ccaatcaccg tggcgcatac
    15241 ggtttgcgcc ggaaactgca cattcatctc acgcaggcgc ggatggtcat gcagcgccgt
    15301 tgccagaagt gggccaatat cggcaaaaca gaatgggtcg gcgggctggt ggcgataaca
    15361 ttcgcccaca cctccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc
    15421 ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg tcaatcactt cgacaatcag
    15481 cgccgccatc cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcagt
    15541 gcctgcaccg aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg
    15601 cccctggctg tcggtttcca gcaggcgacc accgacgttg agacaggcag tgccgctgat
    15661 ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg ccgatatcga tattcagtac
    15721 ccgacacagc cgttgttcag aaagggtttg cgccccggct ccgtgaccgg cgatcacgga
    15781 ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc agcgactgtt tttccttcat
    15841 cactttttcc atcagctgtg cgaccaggct ttcggtaaac tggccttccg gcagctgggc
    15901 aatgatggtt tcgcggatgc gctggctttc ggttttgtcg gctggcacgc tggcgggcac
    15961 ggacgccgtt accgggatcg attcatcaca ttcggtgatg gtaaaaccca gtagatccga
    16021 cacttcacgg gcttccgggg taataatgct ggcgcgcaga accacggaca ttgcctgttc
    16081 gccgcgcgcg tgtgcttcac gaatatcatt cgctgtgatg agttttttca cctgcttcct
    16141 cctgtttggt gagagatgcc agataatcca ccagttgctg cacgctttgc gggtcgtggc
    16201 tgtttagctc gaaaatcggc tcttcgaaac ccgtctcaag caataacttt cgcgttgcgg
    16261 cgacgtcagc atctggcatg tccgttttac tgatgacggc gatttgccgt ttgctgacgc
    16321 caatatccag caacccggca ggcaaccgac tttccgggtc atttgcgcca tggacataga
    16381 tcagcatgtc tacatcctgt agcgtggtaa ttaaggcgtg ataccagcgg ggatggctaa
    16441 aatattcacc cggcgtatca atatcgccct tatcattaaa ttccacggcc tgtgtttttc
    16501 tggcgagggt ataattcccc tgcaacgcat taaataacgt tgtttttcct gccccgacgc
    16561 tacccacaaa cgcaattcgt ttcatgaatg cctcgtatta acttttggtc atttcacaaa
    16621 gcgtataatt taataagcga cctaaaccgc tgacggtttg tgataacgct tcttctactg
    16681 cgcccaccga gccataaatc accaatgcac cgctgaagcg gtcgagaaaa ccgatatgca
    16741 catcagcggc tttcagcgct aaatcaccgg cgatcatcgc cgtttcgccg ggagttagcg
    16801 tcataatgcc aattgcgccc gcatccggaa cgccgatttt cttcgccagt tcctcaccgg
    16861 gatgcgcgat gagatgcgcc agtgtgacct gtttgccggg cacaaattcc tgaatgatgc
    16921 gttctttatc catgagtcgc cgcctgtgaa aaactttcgt gtatcgtgac aatttttcgt
    16981 cagagtaaat aacaaaattc ggcaagcgcg tttaaaaggt gaggtagatc actaagaatg
    17041 gagagatatt tactgttatg aataaacttt catatgcgac gcatgaatat aaataaaata
    17101 atatcgagat attttatata ctgatttttt tattattcgc aaactcagta ataagctttg
    17161 ctaatatatt tcaggaaaac cgcttaccct gaatattcag ggtaagcgtg agagttaaga
    17221 atgaattaca gcggttgggt ttgcgcttca accacggcca gcgccaccat attgacgata
    17281 cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt tcgccacgcc catcagcacc
    17341 ggaccgacag tcacaccttc cgagctggaa acgcgcagta agttgtaact aatgcgggca
    17401 gcttccatgt tcggcatcac cagaatattg gcagaacctt tcaaagggct gtccggcata
    17461 cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt gcatttcacc atcaatcatc
    17521 agatctggcg cacgatccct gaccagttcc agcgcctgac gcattttgct cgacgacggg
    17581 cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa cgcgcggctc aataccaaaa
    17641 cgacggacag tttctgccgc catcaaggtg atctccgcca gctcttctgc gtctggttcg
    17701 tcattgacgt aggtatcggc aacaaaggtg ttaccactcg gcagcagcag cgcgttcatt
    17761 gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat ttttcaccac gctaaaatgt
    17821 tcatgataat cgcccaccgt accgcagatc attgcatcgg cttccccacg ctgaaccatg
    17881 atcgcgccga tcactgtcgg gttactgatc agcgcacgct gcgcctgttc ctgagtgaca
    17941 ccgcgacgct tcatgatctg gaagtattcg gtccagtact ctttaaagcg cggatcggat
    18001 tcgttattga cgatttcaaa atcaacgccc gctttgatct gcaagcccag tttctgaatg
    18061 cgcatttcga tcacgttcgg acgaccgata aggatcggtt tcgccagtcc cagcgttacc
    18121 agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt ccggcagaac tacgcgcttc
    18181 ggcgctttgc gagcctggga gaaaatcggc ttcataaaca ggttggtttt gtaaacgaac
    18241 tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg gacgcgtcgc cacgcccgac
    18301 tccatcgccg cttttgcgac cgcaggagcg atcttaacga tcaagcgcgg atcaaacggt
    18361 tttggaatga tgtattccgg accaaagctc agatcctgaa cgccatacgc tgaagccacc
    18421 acttcactct gttctgcatg ggccagttct gcaatcgcgc gtaccgccgc cagtttcatc
    18481 tcttcgttga tggcggttgc gccaacgtca agcgcgccac ggaagatgaa cgggaagcaa
    18541 agaacgttgt tcacctggtt cgggtagtcg gaacggccgg tacagatgat ggcatccgga
    18601 cgcacttctt tcgccagcgg cggcagaatt tccggttccg ggttcgccag cgccaggatc
    18661 attggtgccc gagccatttt cttcaccatt tcctgggtca gcactttcgg gccggaacag
    18721 cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg tacgtttgcc gtcatccacc
    18781 accgcatatg cggctttggt ttccgccata tttggctcac gcccctgata gataacgcct
    18841 tttgaatcgc aaaccacgat gttatgtttt tgcagcccca gcgctaccag caggttcata
    18901 caggcgattg ctgcggcacc cgcgccggaa accaccatcc gcacgtcgga gatgtttttc
    18961 tccaccacgc gcaagccgtt gaggatggcg gcagtgctga tgattgccgt gccgtgctga
    19021 tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt tctgttcaat atagaaacat
    19081 tctggcgctt taatgtcttc gaggttgatg ccgccgaagg tcggttcgag cgcggcgacg
    19141 acttcaataa atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac atcaatccca
    19201 gcgaatttct taaatagaac gcccttgcct tccatcaccg gtttgcctgc cagcgcgcca
    19261 atgttgccta accccagcac cgccgtaccg ttagagatca ccgcaaccag gttaccgcgc
    19321 gcggtatatt tgtaggcttt tagcgggtct ttttcgattt caagacaagg tgcggcaacg
    19381 cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc
    19441 tggattttcc ctggaactgg aaattcatgg aaatcaagtg cactttgttt taactggtca
    19501 tccatttggt tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct
    19561 gcccacaacg tggcgcacat tattaccctg ccggagtcta cagactttga gcaagtccaa
    19621 actctcacca ttaatataat gttttggtaa caatcctata acactgatat tacctgctta
    19681 atccagcaat accatgcctg tctgctatgc ttttttgatg cgtttagcga aatttctcag
    19741 aagtgtgaat taacgcactc atctaacact ttacttttca aggagtattt cctatgaacg
    19801 agttagacgg catcaaacag ttcaccactg tcgtggcaga cagcggcgat attgagtcca
    19861 ttcgccatta tcatcccctg gatgccacca ccaatccttc gctgttactc aaggctgccg
    19921 gattatcaca atatgagcat ttaatagacg atgctatcgc ctggggtaaa aaaaatggca
    19981 agacccagga acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg
    20041 aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt tgatgcacgc ctctcttttg
    20101 ataaagaaaa gagtattgag aaggcgcgcc atctggtgga cttgtatcag caacaaggcg
    20161 ttgagaaatc acgcattttg atcaaactgg cttcgacctg ggaaggaatt cgcgcggccg
    20221 aagagctgga aaaagaaggt attaactgca acctgacgct gctgttttct tttgcccagg
    20281 cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc gtttgtaggg cgtatttatg
    20341 actggtatca ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta
    20401 aatcggtgcg caatatctac gactactata agcaacacca ctatgaaacc attgtgatgg
    20461 gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat cgactgacta
    20521 tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc tccagtggta cgtaaattaa
    20581 tcccgccttc tcagacgttc ccacgcccag cacccatgag cgaagcggag ttccgttggg
    20641 agcacaatca ggatgcgatg gcggtagaaa aactgtctga aggcattcgt ctgttcgctg
    20701 ttgatcaacg caaactggaa gatcttcttg ccgccaaact ataaaccagc cacggagtgt
    20761 tatatgtccc gaaaagacct tgccaatgcg attcgcgcac tcagtatgga tgcggtacaa
    20821 aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc cgaagtgctg
    20881 tggaacgatt ttcttaaaca taatcctacc gacccaacct ggtatgatcg cgaccgcttt
    20941 attctttcca acggtcacgc gtcgatgctg ctctacagtt tgctacatct gaccggttac
    21001 gacctgccgc tggaagaact gaagaacttc cgtcagttgc attcgaaaac cccaggccac
    21061 ccggagattg gctatacgcc aggcgttgaa accaccaccg gcccgcttgg acaaggtttg
    21121 gcgaacgccg tcgggctggc gatagcagag cgtacactgg cggcgcagtt taaccagcca
    21181 gaccatgaga tcgtcgatca cttcacctat gtgtttatgg gcgacggctg cctgatggaa
    21241 ggtatttccc acgaagtctg ttcgctggca ggcacgctgg gactaggcaa gctgattggt
    21301 ttttacgatc acaacggtat ttccatcgac ggtgaaacag aaggctggtt taccgacgat
    21361 acggcaaaac gttttgaagc ctatcactgg catgtgatcc atgaaatcga cggtcacgat
    21421 ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg tgaaagataa gccgtcgctg
    21481 attatctgcc gtacggtgat tggctttggt tcgccgaata aagcaggtaa ggaagaggcg
    21541 cacggcgcac cactggggga agaagaagtg gcgctggcac ggcaaaaact gggctggcac
    21601 catccgccat ttgagatccc taaagagatt tatcacgcct gggatgcccg tgaaaaaggc
    21661 gaaaaagcgc agcagagctg gaatgagaag tttgccgcct ataaaaaggc tcatccgcaa
    21721 ctggcagaag agtttacccg acggatgagc ggtggtttac cgaaggactg ggagaaaacg
    21781 actcagaaat atatcaatga gttacaggca aatccggcga aaatcgctac ccgtaaggct
    21841 tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg agttgctcgg cggttcggcg
    21901 gatctggctc ccagcaacct gaccatctgg aaaggttctg tttcgctgaa ggaagatccg
    21961 gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat cgccaacggc
    22021 atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt tgttgaatac
    22081 gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc ggcagattat ggtttatacc
    22141 cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga gcaactggcc
    22201 agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcaggt ggaagcggcg
    22261 gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat cctctcaagg
    22321 cagaatctgg cccaggtgga acgtacgccg gatcaggtta aagagattgc tcgtggcggt
    22381 tatgtgctga aagacagcgg cggtaagcca gatattattc tgattgccac cggttcagag
    22441 atggaaatta ccctgcaagc ggcagagaaa ttagcaggag aaggtcgcaa tgtacgcgta
    22501 gtttccctgc cctcgaccga tattttcgac gcccaggatg aggaatatcg ggagtcggtg
    22561 ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga ttactggtac
    22621 aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg gttacgggga atctgctccg
    22681 gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc aaaagcgcat
    22741 aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgtatg ttcccctcac
    22801 cctaaccctc tccccagaag ggcgagggga cggtccgtgc gctttttagg gccgcatctg
    22861 cgattttgtt taacgggtga tcacaacgtg ggcctggcgt ctggcccatg ccagttatcg
    22921 caggtaggtt ctgcggcgta acgcaccagg cgaaaactct gaccgtcaaa gcgccagcgc
    22981 gcctgaatgc cacaatcgct taatccgcgc cctttcgcta aggtcaccag ttcacgcgat
    23041 ttctcatcaa atgttgcgtt catcagttcc agttcattcg tctcctgacc gttgttgaac
    23101 ggcaaacgca accgaaccgg gcgcgaggct agcggctttt tacgcgacac aatccatgcc
    23161 aaatcaatgg tgttataggc ccctgcctca cagctaatca tcatcagtgc tttatcatcg
    23221 gtcagcgcag tgacattcac ctcacgacgt aacggatcaa gcgagcagcg cagaccattc
    23281 atccgccagt ttccataatc cagcaaatcg ttacgttctt cgagtgagag tggcgtcggc
    23341 gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg gcggtacgct gagaggcggt
    23401 tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc gcttttgctg agcatcaata
    23461 aacaacaacg ccgcttttaa gccgctcaga gaaatggtct gattgccgtc gcgtaaggtg
    23521 atcgccttcc cttcctgaat tatctgcaaa aacgcggtga tggttgccgt atcatcggtt
    23581 actaataacc atggtgaaat ccgccacttg tcaccactta atgccaacgg ctcaccatct
    23641 aatagcagcc gtggcgctat ctccccttca gacgcatccg gcgacttcaa cccaccgcgc
    23701 tcaatacgta aaacggcatc ggtatgcgcc ccagcgctgc ggctcagggt catcaccagt
    23761 ccattatgat cgcccgtatt gcgcgccaca caaaaatttt gattattaca ggtgacctgc
    23821 cagtcggaaa acgcccgttg cgctggtgcc gcccacacca aagacgtcgg caacaaagcg
    23881 aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta cgaccccaga agttcaaaca
    23941 aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat ttgtcggatt
    24001 gagccaataa taaacaacga aatattattg ctatttttca gtccattaaa tgtgacattt
    24061 gcaaatagtt aagcaataac accgtcttac cgtcacgtat ctcgccggtt ttgatcatct
    24121 ccagcgcctg gctgaacggc agctcgagca cttcaatatc ttcatcttca acaccgccac
    24181 cggcgttagc gcgctgattg tcactgtatt cggcgataaa aaagtggatt agctcagtca
    24241 caccgccagg cgacatatac agttcaaata atttgcgcac ttcgcccact tcatagcccg
    24301 tctcttcaat cgcttcttta cgaatgcaca cttccggttc gtcgttatcc agcagcccgg
    24361 cgcaggtttc aatcagctgc ccgctttcat tgccattaac ccaggtagcg acacggaact
    24421 gacgaatcag aaccacgctc tttttcttcg cgttgtacag gaggatcgtc gcaccattac
    24481 cgcgatcgta aacttcacgt ttatggcgga taacttcacc atctttgcgc gtgagatcgt
    24541 aagtaatgtt gtgcagggtg aaatagttat cggagagaat tttgtcttta atgagggtga
    24601 tttgttgcgt cataccgact ccacagcgcg aaatgaacaa ttatattacg ctgtgaagtc
    24661 gggtttgtct gcgcaggcta tcagtgagat ttgactgatt ttacccccag ccagtcaata
    24721 atcccctgtg ccgcatgacg tccttctgcc attgcggtaa ccaccagatc cgcaccacgc
    24781 acggcgtcgc caccggcgaa gattttcgga ttggtggtct ggtaacggta ctggctttcc
    24841 acatccgcga tgatgcggcc ccatttgtct accgttacac cgtgcgactc cagccacggc
    24901 atcccgtgtg gattgaagcc aaacgccata atcaccgcat cggctggcat gacaaattca
    24961 ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat ccggctcgcc aagacgcgtg
    25021 cgcaggaaac gaattccgca aacgtgacct tgctcattca gctcaagcgc caccggctgg
    25081 acgttaaatt cgaagttggc cccctcttcg cgggcgttct tcacctcttt cttcgagcct
    25141 ggcatgttag cttcatcacg acgataagcg caggtgacgt tactcgcgcc gtggcgcagc
    25201 gcagtacgca cacagtccat cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg
    25261 gcggtattga taaacggctc ttccggtagc tcttcgagcc ccatcacctg tttagtgttg
    25321 gcaatgatga acggcagcgc gtcataaacg cccggcgtat cttcattggg taaacccgct
    25381 ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc caaaagcgaa
    25441 tccaaagaga catctttacc cacttcacaa ttgagttcga agtgaatccc catcgcgctg
    25501 aagatttccc gacggcgtgc cagcagggat ttatccagtt tgaaagaagg aatgccgaaa
    25561 gtgagcaagc caccgatttc tggatggcga tcgtacaccg tcaccgccac gccattgcgg
    25621 gtcagaacat ccgcacaggc cagccctgcc ggacctgccc cgataatcgc cacccgcttg
    25681 tccactttgg tgacatgact taagtcagga cgccaacctt tcgccaacgc ctgatctgaa
    25741 atatagcgtt caatgttgcc gatagttacc gcgccgtgct catcgcgaat agtacaggca
    25801 ccttcacaca aacggtcttg cggacaaacg cgtccggtaa tttccggcag ggtgttggtc
    25861 tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt tcaccagttc aatccactgc
    25921 ggaatatggt tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc gaacttaagg
    25981 cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg gcagataaat ttcatcaaaa
    26041 ccggttttgc gcgcttcaat cgccagttta tccggctcgc cacgcgcggg cgttgcctgc
    26101 atttgttcga ctttactcat taccggcatt tcttgtgcag cggtactggc atgccacggt
    26161 tgatgctcct gacgtgcggt gcgcaagcgg cgggatttcg ccatgccgga gagtgcgacg
    26221 tcagtgacca gttgcagcgc gtccgccggg caattctcga cacaggcagg accgttttcg
    26281 cgccctgcac aaaggtcgca tttatgcgcc gtggctttta cttttcctgc cgcgacgggt
    26341 gtcaggacga tttgcatcgt accaaacgga caggccacca cgcaggattt acagccaata
    26401 cacttttgct gattgacctg aatgctgtca tcaacgtggc tgattgcgcc attagggcag
    26461 ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca ctgcactacg ttgctgctga
    26521 tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg ttgctcatca
    26581 ttgtgagcca tgacacaggc gatttcacaa gcatgacaac ccagacattg ctgactgttg
    26641 gccataataa aacgattcat aacgaccttc ttttttggtt gtaaaaacct tattctttat
    26701 atgagtgttg ttattacccg acttacaggg gaaccggcaa tgttcatgtg cccagaataa
    26761 gtaactattt cgctataaac tgtggcagat caaataatcc catcactgac taaattgcgt
    26821 ttcagcgaac tggaacatta atgatttttt gtggagaaga cgcgtgtgat tgttaaacgc
    26881 cccgtctcgg ccagtctggc ccgggccttt ttttacattg tgctgctgtc gattctttcc
    26941 acgggtatcg ctctgctaac tctggcgagc agtttgcgcg acgctgaggc tatcaatatt
    27001 gccggatcgc tgcgtatgca gagttaccgc ctgggctacg acttgcaaag tggcagtcca
    27061 caactcaatg cacatcgcca gttgtttcag caggcactgc attcaccggt attaaccaac
    27121 ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct atgcgcatct gaatgccaac
    27181 tggctggaga tgaataatcg gctcagcaag ggcgatttgc cgtggtatca ggccaatatt
    27241 aataattatg ttaatcagat agacctgttc gtgctggctt tacagcacta cgctgaacgc
    27301 aaaatgctgc tggtggtggc gatttccctg gctggcggta tcggtatttt cacgctggtc
    27361 ttttttactt tgcgccgcat acgccatcag gtggttgccc cgctgaatca gctggttacc
    27421 gccagtcagc gtattgaaca cgggcagttc gactcgccgc cgctggatac cagtctgccg
    27481 aatgagcttg gtctgcttgc aaaaaccttt aaccaaatgt cgagcgagct gcataaattg
    27541 tacctttcgc tggaggcgtc agtagaagaa aagacccgcg atctccacga ggccaagcgt
    27601 cgtctggagg tgttgtatca gtgcagtgtt cccaggcgct gaacaccagc cagattgatg
    27661 tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcagct gaatatctgg
    27721 agctgaacgt tggggataac tggcggatta gcgaagggaa gccaaaccct gagcagccaa
    27781 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
    27841 gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg ggacgcggtt
    27901 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcga
    27961 ccatcgcccg cgaattgcac gactcgctgg ctcaggtgct ttcttactta cgtatccagt
    28021 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
    28081 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actactttcc
    28141 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
    28201 aaaatcaaac cagcgctaaa ctgaccctcg actgccgtct gccaaccctg gcactggatg
    28261 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
    28321 acgccaacgc cagcgaaatc gccatcagtt gcgtcaccgc gccggacggc aatcacacgg
    28381 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
    28441 gactgaatat catgcgcgaa cgcgcggaac ggctgggcgg gacgctgact ttttcacaac
    28501 cttccggcgg cggcacgtta gtgagtatta gctttcgctc cgcagagggt gaggggaagg
    28561 ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc ctctcccttt
    28621 aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc agcaatctca
    28681 tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg ccgctacagt
    28741 gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta atggcgaatt
    28801 tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt ctgacaggta
    28861 ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca ccgaatgtgc
    28921 gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg
    28981 ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct cagagcagtg
    29041 gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg gacgaagccg
    29101 tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa
    29161 atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt gccttcgtct
    29221 ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt aatattcagg
    29281 acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg caatattcca
    29341 tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct aaagatgtca
    29401 ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt
    29461 ccgtcgataa gcaggcgctc aacgccgcca ttaacgccca gtcactgctg caaacaccag
    29521 aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta aggctgggcg
    29581 atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc ttcaatggta
    29641 agccagcctc cgggttgggg gtaaaactgg cctccggcgc aaacgaaatg gcgacagcgg
    29701 agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga ctggaataca
    29761 aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc
    29821 tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa aacttccgcg
    29881 ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc tccgtacttt
    29941 acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc
    30001 tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg agtgaggaag
    30061 gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg gcactggtcg
    30121 ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc ggcaccaccg
    30181 gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg tcagtactgg
    30241 tagcgatgat cctcactccg gctctgtgtg ccacgctact taagccactg aaaaaaggtg
    30301 agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac cgcaacgccg
    30361 aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg attgtgattt
    30421 atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg tcgttcttac
    30481 cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt tcaacccaac
    30541 aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcatccat gaaaaagaca
    30601 acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg caaaacgtgg
    30661 cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc ggcacctcgt
    30721 ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct cgcgttatcg
    30781 ccagcagccc accagcaatt agcggtcttg gtagttctgc agattttgat atggagttgc
    30841 aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag ttgctggcgc
    30901 tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat gacagtccgc
    30961 agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat atcgacgata
    31021 ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt atggatcgcg
    31081 gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca
    31141 tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct gctttcgcga
    31201 cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacagttat tctgcggttg
    31261 agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat attatggaat
    31321 cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg tcgtatcagg
    31381 agcggctttc cggcacgcag gctccggcgc tgtacgccat ttccttgctg gtggtattcc
    31441 tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg ctggtcgtgc
    31501 cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa aacgacgttt
    31561 acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc atcctgatcg
    31621 tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg acgctccacg
    31681 cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc ttcggcgtat
    31741 tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg
    31801 taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg ctgttctttg
    31861 tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga
    31921 catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc gggttcttgc
    31981 tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag tctttccagt
    32041 tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt ctacggaaaa
    32101 aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc gggggacaaa
    32161 ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca gcaacatttc
    32221 gagatattca tacggcatct aatacttatt taattctggt taaaatacag acagataaca
    32281 agatgaatat tcttaatgtt tacgttaaaa atgtttaata ttatttaata gttgttaatt
    32341 tgaatacttc gataatgtta tattttctga taatcatttg caggcaaaat gttttcaccc
    32401 ttaaatgagt atttattctc ataaatcgaa aaaggattca ttatggttac actttacggc
    32461 atcaaaaatt gtgacaccat taaaaaggct cgccgttggc tggaagccaa taacatcgac
    32521 tatcgttttc atgattaccg cgtcgatggg ctggacagcg aattattgaa cggttttatc
    32581 aacgaattag gctgggaagc gttactcaac acccgtggta caacctggcg taaactggac
    32641 gaaactaccc gcaataaaat caccgatgcg gcctctgcgg cggcattaat gactgaaatg
    32701 cctgcaatta tcaaacgtcc attgctctac gcgcctggta agcctatgct gctgggtttc
    32761 agtgattcca gttatcagca atttttccat gaggtgtagt ctatgtcgtg cccggttatt
    32821 gagctgacac aacagcttat tcgccgccct tccctgagtc ctgatgacgc aggatgtcag
    32881 gctttgttga ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat ggattttgcc
    32941 gatacgcaga atttttgggc atggcgtggg caaggtgaaa cgttagcctt tgccgggcat
    33001 accgacgtgg tgccgcctgg cgacgccgat cgttggatca atccgccgtt tgaacccacc
    33061 attcgtgacg gcatgttatt cgggcgcggt gcggcagata tgaaaggctc gctggcggcg
    33121 atggtggtag cagcagaacg ttttgtcgca caacatccca accatacggg gcgactggca
    33181 tttctgatca cttctgatga agaagccagt gcccacaacg gtacggtaaa agtcgtcgag
    33241 gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc gtcgagtatc
    33301 gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg caacctcacc
    33361 attcatggcg ttcaggggca tgttgcctac ccacatctgg ctgacaatcc ggtacatcgc
    33421 gcagcacctt tccttaatga attagtggct attgagtggg atcagggcaa tgaattcttc
    33481 ccggcgacca gtatgcagat tgccaatatt caggcgggaa cgggcagtaa caatgttatt
    33541 ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca ccgaactgac tgatgagatg
    33601 atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac tgcgctatac ggtggattgg
    33661 tggctttccg ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc ggtcgttaac
    33721 gcggttgagc actataatga aattaaaccg cagctactga ccacaggcgg aacgtccgac
    33781 gggcgcttta ttgctcgcat gggggcgcag gtggtggaac tcgggccggt caatgccact
    33841 attcataaaa ttaatgaatg tgtgaacgct gccgacctgc agctacttgc ccgtatgtat
    33901 caacgtatca tagaacagct cgtcgcgtga tgagtggttc tgcaagagga aataagcatg
    33961 gactggctgg ctaaatattg gtggattctg gtgattgtct ttttggtcgg cgtcctgctg
    34021 aacgtgatta aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa caagccggag
    34081 cttcccccgc atcgtgattt caacgataag tgggacgatg acgacgactg gccgaaaaag
    34141 gatcaaccga agaagtaagc tgagtgctca accgtaggcc ggataaggcg ttaacgccgc
    34201 atccggcaat ggtgaacgat gcctgatgcg acgctcacgc gtcttatcag gccaggtaat
    34261 ctgtctttta catcatttca atcacatcat catcgccagg cttaccgcca cttagcgctt
    34321 ggtcgaaata atgtttcgga atggtataac gcaagtgatc cagggcaaat tgcatactgc
    34381 gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca ccaccggcac
    34441 tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc gataaccgga tcttcaccac
    34501 catgaatcaa gtgaattgtc gtggctgtag acgcggtctc cggcaggctc gcatagcgtc
    34561 cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc tccagcgcca
    34621 taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact ccgctctgtt
    34681 tctgccagta gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca cgcgcctggc
    34741 gattatcttc cgtaataccc tgcactgaaa accactgacg ccccgccgga ttaccgctcg
    34801 gctccgcgcc accgacactc accaccagcg catcaggaaa cagcggtgca aaccagctgc
    34861 ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt aacaactgtt
    34921 gtgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg aactccttaa
    34981 ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa ttgaaggagt
    35041 cagtgaaaaa attgccattg cgttatgcga tcgcgcagac gctcagtgcg aacctcattt
    35101 agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat cttgcggcct
    35161 gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa acacccacgc
    35221 agcgccggta atgccagttc actggtttgt agcagacgca ataagcaacc taacgatgtt
    35281 aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc gtcagaaagg
    35341 atggcatcgt ttagtggatc aacaggaagc atttcgccat tccactgcgc gagagcttgc
    35401 gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc atcactcatc
    35461 ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cccgatgatt acccatccgc
    35521 accagcacaa aaccgcagcg ttgccagaaa cgccataact ccccagtgta accaaaactc
    35581 accgaaagat agtcgaggtc atgcgtatat tgcaaagctc cagcaataag ttgccgccct
    35641 gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct tccgcgcaat
    35701 gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag attaccccgc
    35761 gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc gccctcatcc
    35821 accagccaca acgccccggc aatctcgttt tcgccagccg cctgtaaaaa atgttgccct
    35881 agtgcatcca tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc accagacaat
    35941 aactgataaa cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg ttcaaatgcg
    36001 gaaatgacga tattgccttg tggtgtatgg gtgaagtttt catcgttaaa aaccagtgcc
    36061 tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg ttgcagttca
    36121 aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg tccggtacct
    36181 tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac cagttgatgc
    36241 a
//
LOCUS       DADLTI010000019        35842 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLTI010000019 DADLTI010000000
VERSION     DADLTI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.019"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(47..4664)
                     /locus_tag="JN755_000890"
                     /pseudo
     CDS             complement(47..4664)
                     /locus_tag="JN755_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4757..5404)
                     /gene="rnt"
                     /locus_tag="JN755_000891"
     CDS             complement(4757..5404)
                     /gene="rnt"
                     /locus_tag="JN755_000891"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7379776.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5507..5914)
                     /gene="gloA"
                     /locus_tag="JN755_000892"
     CDS             complement(5507..5914)
                     /gene="gloA"
                     /locus_tag="JN755_000892"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7379777.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5995..7092)
                     /gene="nemA"
                     /locus_tag="JN755_000893"
     CDS             complement(5995..7092)
                     /gene="nemA"
                     /locus_tag="JN755_000893"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7379778.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7129..7728)
                     /locus_tag="JN755_000894"
     CDS             complement(7129..7728)
                     /locus_tag="JN755_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7379779.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7831..8070
                     /locus_tag="JN755_000895"
     CDS             7831..8070
                     /locus_tag="JN755_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7379780.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8119..9015
                     /locus_tag="JN755_000896"
     CDS             8119..9015
                     /locus_tag="JN755_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7379781.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9096..9617
                     /gene="sodC"
                     /locus_tag="JN755_000897"
     CDS             9096..9617
                     /gene="sodC"
                     /locus_tag="JN755_000897"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7379782.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9618..11600)
                     /locus_tag="JN755_000898"
                     /pseudo
     CDS             complement(9618..11600)
                     /locus_tag="JN755_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11593..12384)
                     /locus_tag="JN755_000899"
                     /pseudo
     CDS             complement(11593..12384)
                     /locus_tag="JN755_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(12387..12623)
                     /locus_tag="JN755_000900"
     CDS             complement(12387..12623)
                     /locus_tag="JN755_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7379783.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12824..13258
                     /gene="slyA"
                     /locus_tag="JN755_000901"
     CDS             12824..13258
                     /gene="slyA"
                     /locus_tag="JN755_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7379784.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13305..13772)
                     /gene="slyB"
                     /locus_tag="JN755_000902"
     CDS             complement(13305..13772)
                     /gene="slyB"
                     /locus_tag="JN755_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7379785.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14046..15155
                     /gene="anmK"
                     /locus_tag="JN755_000903"
     CDS             14046..15155
                     /gene="anmK"
                     /locus_tag="JN755_000903"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7379786.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15253..15582
                     /gene="mliC"
                     /locus_tag="JN755_000904"
     CDS             15253..15582
                     /gene="mliC"
                     /locus_tag="JN755_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7379787.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15641..16297
                     /gene="pdxH"
                     /locus_tag="JN755_000905"
     CDS             15641..16297
                     /gene="pdxH"
                     /locus_tag="JN755_000905"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7379788.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16426..17700
                     /gene="tyrS"
                     /locus_tag="JN755_000906"
     CDS             16426..17700
                     /gene="tyrS"
                     /locus_tag="JN755_000906"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7379789.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17762..18628
                     /gene="pdxY"
                     /locus_tag="JN755_000907"
     CDS             17762..18628
                     /gene="pdxY"
                     /locus_tag="JN755_000907"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7379790.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(18672..19277)
                     /gene="gstA"
                     /locus_tag="JN755_000908"
     CDS             complement(18672..19277)
                     /gene="gstA"
                     /locus_tag="JN755_000908"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7379791.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19383..20885)
                     /gene="dtpA"
                     /locus_tag="JN755_000909"
     CDS             complement(19383..20885)
                     /gene="dtpA"
                     /locus_tag="JN755_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7379792.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21496..22131)
                     /gene="nth"
                     /locus_tag="JN755_000910"
     CDS             complement(21496..22131)
                     /gene="nth"
                     /locus_tag="JN755_000910"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7379793.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22131..22826)
                     /locus_tag="JN755_000911"
     CDS             complement(22131..22826)
                     /locus_tag="JN755_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7379794.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(22830..23450)
                     /gene="rsxG"
                     /locus_tag="JN755_000912"
     CDS             complement(22830..23450)
                     /gene="rsxG"
                     /locus_tag="JN755_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7379795.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23454..24512)
                     /gene="rsxD"
                     /locus_tag="JN755_000913"
     CDS             complement(23454..24512)
                     /gene="rsxD"
                     /locus_tag="JN755_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7379796.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24513..26351)
                     /gene="rsxC"
                     /locus_tag="JN755_000914"
     CDS             complement(24513..26351)
                     /gene="rsxC"
                     /locus_tag="JN755_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7379797.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(26344..26922)
                     /gene="rsxB"
                     /locus_tag="JN755_000915"
     CDS             complement(26344..26922)
                     /gene="rsxB"
                     /locus_tag="JN755_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7379798.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(26922..27503)
                     /gene="rsxA"
                     /locus_tag="JN755_000916"
     CDS             complement(26922..27503)
                     /gene="rsxA"
                     /locus_tag="JN755_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7379799.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(27580..28020)
                     /locus_tag="JN755_000917"
     CDS             complement(27580..28020)
                     /locus_tag="JN755_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7379800.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(28106..28321)
                     /gene="ydgT"
                     /locus_tag="JN755_000918"
     CDS             complement(28106..28321)
                     /gene="ydgT"
                     /locus_tag="JN755_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7379801.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(28594..28719)
                     /gene="blr"
                     /locus_tag="JN755_000919"
     CDS             complement(28594..28719)
                     /gene="blr"
                     /locus_tag="JN755_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7379802.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            28962..30002
                     /locus_tag="JN755_000920"
     CDS             28962..30002
                     /locus_tag="JN755_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7379803.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30038..31039)
                     /gene="add"
                     /locus_tag="JN755_000921"
     CDS             complement(30038..31039)
                     /gene="add"
                     /locus_tag="JN755_000921"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7379804.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31142..32314)
                     /gene="malY"
                     /locus_tag="JN755_000922"
     CDS             complement(31142..32314)
                     /gene="malY"
                     /locus_tag="JN755_000922"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7379805.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(32324..33916)
                     /locus_tag="JN755_000923"
     CDS             complement(32324..33916)
                     /locus_tag="JN755_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7379806.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34091..35119
                     /locus_tag="JN755_000924"
     CDS             34091..35119
                     /locus_tag="JN755_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7379807.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            35231..>35842
                     /gene="hdhA"
                     /locus_tag="JN755_000925"
     CDS             35231..>35842
                     /gene="hdhA"
                     /locus_tag="JN755_000925"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7379808.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPE"
BASE COUNT         8995 a         9190 c         9305 g         8352 t
ORIGIN      
        1 tgaacgtagc agggaaccac gtctttcagg gcgtggagga tgtcaactat ccccaatcca
       61 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
      121 ggaccggaag atcattcact tctgttagcg taaagcgcag ccttggttcg cgacgcagtg
      181 cggtggtcag cgcctggaaa atctctgggg cgagtaattc ctccttttcc tgccagacca
      241 gcattttttt gccgccttgc ggcagataga gtaacaattt gccgccagaa acgaccacca
      301 gcgcacccgc ccgacgcgtt ggtaccagcg ctgccggatg tgcaggccag ggcagaagat
      361 ttccccacac attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgtgttt
      421 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
      481 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
      541 gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
      601 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat
      661 cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag
      721 atacccgcgg cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc
      781 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
      841 tatcgctggt aatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg
      901 aatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
      961 ctcctccgtc agccagaaca gcgattatcg cttgctgcag cgccgaacga ttcgcctgat
     1021 ccgcttcggc gggagtgaac gattctgcgg catatttctg tagatgcagt gccaccaggc
     1081 cgtcatcttc acccagcttt tttttgtccc gaccagataa ccgctccggt tgccagtaat
     1141 tcatcgagca tttctggcga atagtcgcgt acgcgggcag gcaggatctg gctttcccag
     1201 agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca cccgcatcac gccatctacg
     1261 ccctcataaa cgcctggcga tgttgaggca aagagcgccg ggctaccatc ggtggcgggt
     1321 aatacgccct gacgttccag cagcaatcgc gcataggtcg tggctgcaac gggacgcgtc
     1381 gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac gaaatacttc atcgctcacc
     1441 cagatgtctt gttgcagatt catcaccaga ccctgttcac gcagttgctg aagctgctct
     1501 tcgacaatgg caataccgag actaaactca tgagccagtt gttcagccgt gaccagagca
     1561 tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt aactgactct atgtaaataa
     1621 atctctggca atgactctgg tagtcgtacg ccgagggcat cacgcagcct ggcggcatca
     1681 tccatacagg caagacgctc ctgtccgcta atcatcgcag ggaagattcg ttttactgca
     1741 agaagatttt ccagatacga cgcaacctct tcactgctgc ctgtatgccg ttgcgccagg
     1801 tcttcaacgg tcattggccc cagttcgcgc agcaggtcga acaatccttc ttcacctttc
     1861 gctcttctgc caggagccag tcgttgcaac tcttcttcca cctggcgaat gacctgcggg
     1921 tcgagtaatt cccccggatc gacctgtccg agtagattgc gcagtaactc gctgtccagc
     1981 gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc tctggtacat aaattccgcg
     2041 acatagccga acaataaact tgtggcgaaa ggcgagggcg tagtggtcgt tatatcggat
     2101 atttgaattt cgccaccgtt caggcgacgc atcaaacgtt ccagtgcggg aagatcataa
     2161 acatcttgca gacattcgcg tagggtttcg agaatgaccg gaaaatccgg atatccctga
     2221 gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc aaagcggggt gcgatggccc
     2281 ggcgtgcggc ccggcattaa taatgcccgc gcggcgcatt cgcgaaaacg ggcggcgaaa
     2341 agtgccgagc tgcctaccgc ctcgcggaca atttgcagca acttttctgg ttcaaacaaa
     2401 aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa tgcgcgcaac aatgccgtca
     2461 tcactggcga ccacagacgc gtcagcgccc catagggcat gtattcgccc ggcaatcgcc
     2521 agcgcccagg gttcatgcac ccgtcttcca tagggagagt gcaaaataat acgccagtcg
     2581 ccaatttcat cacggcaccg ttcgaggacc agatggcgac tccccggaac gatgcccgtc
     2641 gcgttgcgtt gctcgtcaat caacccctga atattggcgt tcgtattttc ttctgccagc
     2701 cacgggggaa tagtcccgga aaagaacgcg ccatccgcca gtaaatgaag aaaatcgccg
     2761 atcatctcgc ctaattcagc cggacgtccg ttaccttcac cacgccagaa ggggagccgg
     2821 gcagaacgac ccggagcagg agtcacaatc acctgatcgc gggtgatttg ctggatccgc
     2881 catgaggtag cgccgagagt gataatgtcg ttcacccgcg actcatatac catctcctca
     2941 tccagttcac ccacccgccg cgaaccggcc ttttcttcac cttcgggtaa taacacgcta
     3001 tacatgccac gatccggaat ggtgccgccg ctggtaaccg ccaacaattg agcgccaggt
     3061 cgggcggtca atatcccggt ctccctgttc cagaccagtt tggggcgaaa agcagaaaaa
     3121 tcgccagagg gatagcgccc ggaaagcata tccagcgtgg cgtcaaaaac acgtcttggc
     3181 agatctttcc acggtgcggc acggcgtacg cgggagtacc attcgtctac ctgtaatgca
     3241 tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt cgagaggatt atgcggtggt
     3301 gtcaggtttt ccagcctgcc tgcgaacata cactctacaa tgactgcgga atcgactaaa
     3361 tcacgccggg tacgggggaa aaacagccct ttagatacac cgccaacctg atgtccggcg
     3421 cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg ttgccacctg aatcaccaga
     3481 tccaccgcgc ccatatcaat ccccagttca agactggagg ttgcgaccac gcagcgtaat
     3541 tcacccgatt tcagcgcctg ttcggtgatt gctcgttgtt ctttagagac ggagccgtgg
     3601 tgtgagcggg caataaaaac gtcgctactt tgtacacggt tagaggttgc gccggaggtc
     3661 gactcgaaat gggccgcatc aacggcgata gacggggaac gctgtaagcg tgcggcgtaa
     3721 agctcattta atcgtgccgt cagtttttcc gccagcccgc gtgaattagt aaagacaatg
     3781 gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt caatatatgg ccagatggag
     3841 ccttcccggc cggcatggct gtcttcgccg gtgccgctgg cgaccgatga gacatcatcc
     3901 atattggcga ccggtacgac aattcgtatc tgcggatggc gcattgcggg cgggttgact
     3961 accgtaaccg ggcgatcgcc accaagaaat gctgccacat cgctggctga gcgcacagtg
     4021 gcagaaaggc caattcgctg tgctgaggtg tggagcagcg catcgagccg ctccagactt
     4081 aacgccagat gcgcaccacg tttactgccc gctaccgcgt ggacttcatc aataattacc
     4141 gtttcgacgc cgtgtagcgt ttcgcgcgcg cgggaggtca gcatcagata gagtgattcg
     4201 ggtgtggtga tcagaatatc cggcggatta cgggtgagtt tgctgcgttc ctgtgcaggc
     4261 gtatcgccag tacggatccc tacgcgaaga ttgacttccg tttcgccgcg ccgccgccgt
     4321 tcatcggcaa tacccttcaa caggatctgc aagttgcgct gaacgtcggt gcccagggct
     4381 tttatcggtg aaatatagag gatgcgtgag gttttacgct tatgcgcctc gcgggtatct
     4441 tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa atgctgccag cgttttcccg
     4501 gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg ccgccacatg ccaggtttgc
     4561 gactggacag cggtcggctg tttaaaggcg cgaagaaacc agtcgcgggt cgccggtgaa
     4621 aacacgtccg gcaggagcga tgaagggtct ggattatctg ccattaacgc tattccccgt
     4681 gaatatcatg cttgatgcgt ggaatagcct cactatagat gcctgaatca ttgcatgtca
     4741 tcaggcatcg actcgattac acctcttcgg cggcaggtag cggccagcct cccagacgtt
     4801 tccagcggtt gacgatttca caaaacagca cagcagtgcg ttcggtgtcg tacagcgcgg
     4861 agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg gcaagccttt gacaatacgg
     4921 tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa agtggcgaaa gggtggaacg
     4981 ggttacgttt cagcgaggcg cgttctgcgg cggccatcat aaagctgtga tcaaaattgg
     5041 cgttgtgcgc caccataatg gcgcggttac agccgctcgc tttaatacct ttacgtacaa
     5101 ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc gccgcgatcg ggatcgttcg
     5161 ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt tgcgccgaca aatggttcga
     5221 cgtggaaatg taatgtggtg tccggcatca gccagccttg ttcatccatt ttcagggtga
     5281 tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa tccggctgtt tcaacatcga
     5341 tcacaacagg ataaaaacca cgaaaacggt cgcacagacc ggtaagttga gcgttatcgg
     5401 acatctgatt ctcttaatac ggataaattg cagcgcgcat tatgacaaat attacttgtc
     5461 gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc aggagattag ttgcccagac
     5521 cgcgaccggc gtctttctct tcgattaact caattttgta accgtccgga tcttccacaa
     5581 acgcgataac cgtagtaccg ccttttaccg gacccgcctc acgggtcacg ttacccccgt
     5641 tttgacggat tttttcgcac gcttcagcgg cgttatctac gctaagcgcg atgtgaccat
     5701 aagcagtgcc gagttcgtat ttatccacgc cccagttgta ggtcagttca atcaccgctt
     5761 cttcggtttc cgggccgtag ccaacaaacg ccagtgagta tttgtattcc gggttttcgc
     5821 tggtacgcag cagtttcatg cccagcactt tggtataaaa atcgatggag cgttgcaaat
     5881 cgccaacgcg cagcatggta tgaagaagac gcatcttttt atcctcaaaa tggctaacag
     5941 attcaaaatg ttgttttagt atagcggcgg ctttacgccg ctcgcaatgt tggattacaa
     6001 cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg
     6061 gttaagctca gctttgagct gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg
     6121 accaaatgcc accgcatcaa ttaacccttt gccgatcagc gtttcagctt tttctactgt
     6181 gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc
     6241 atcagtatac ggttcacccc ccgcccaatc tggttctgac atatgcagat aagcaatgcc
     6301 gcgtttaccc agttgttcaa tcagatacag tgcatcggct tcttcattcg ggccgttatc
     6361 tgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca atgcgatcgg caccccattc
     6421 ttcaatcccg gcatcgacca cttccagtac caaacgtgcg cgattttcca cgctgccgcc
     6481 gtactgatcg gtacgatggt ttgaagaagg ggaaaggaac tgatgcagca aataaccgtg
     6541 agcagagtgg agctctacca gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg
     6601 gaaatcattg acgatacccg gaatctcttc cagttcaagc gcacgcggca tggatgtttc
     6661 aacgcggatc gcccgaccat tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc
     6721 tgaaggcgct accggtgcct gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt
     6781 gtgccacagc tgcaaggcca tatgaccatt ttcagcatga acgccagcgg tgattttttt
     6841 ccatgcggca atttgctccg gactatggat gccaggcgca cctgcatatc cttttgcctg
     6901 ggcagaaatt tgcgtggctt cactaataat caaaccggca ctggcacgtt ggcgatagta
     6961 ttccgccatc aacggggtag gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg
     7021 tgccataaaa atacggtttg ccgccgtgat cgcgcccact ttcagtgggg aatacagttt
     7081 ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa aatgcctgct aaacggcagg
     7141 cgtcgcaata atgtttttta catgggccag cgcgttttcc agtggctcga aactgcgcga
     7201 aattttggcc tgcagattcg cgccaagcca tagtgcgtaa agcacttgtg cctgttgtag
     7261 cggttcgcca caaaaggtta aacaatggtt ctcacggcca ttttccagag cctgcgagag
     7321 cagggcgatc acgccgcgag cacctttatc catcgcgctg cgcatatctt ctgacagatc
     7381 gcacacttcg gcagagagtt ttactgtcag gcaaccactg atggttccat gttggcaaaa
     7441 ctggttcagt gtttgctggt aataagccag tatgcggtcg cggtagttac cttcgccgga
     7501 ttgcagcaac tcagccagtc gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac
     7561 gccaaacgct tctttagagc gaaagtagtg atagaaggac cctttcggca cttcagcggt
     7621 ttttagtaat tcgcttagcc ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc
     7681 cgtcgccaag agatgttcgc gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt
     7741 agtagaccag tcggtctagt gcaagcaaag ttgctacttt ggacattaac agcttcaata
     7801 atggtaataa tcttatctga ggagggcgag gtggcggagc aattagagtt ctttcccgtc
     7861 cagagcccgt gccggggaat ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt
     7921 cgtagtcggg atgagcgttt taactggaat aaaatgagcg atggcgaaaa acaagaggtg
     7981 ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg caaataagcc agcgtcatca
     8041 gacgaaccgg agcaaccatc actcttttga acctaatctt gcgtatactc ctctcatatc
     8101 tgataagagg aagcgattat ggttcagcgt attactattg cgccggaagg cccggagttt
     8161 tcccgttttg tgatgggcta ctggcgattg atggactgga atatgtccgc ccgccagctg
     8221 gtcagtttta ttgaagagca tctggatctc ggcgtgacca ccgtggacca tgctgatatt
     8281 tatggtggct atcagtgcga agcggcgttt ggcgaggcac tgaaactggc acctcacctg
     8341 cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac
     8401 gtcattggtc attacatcac tgaccgcaat cacatcatta agagcgccga acagtcgcta
     8461 attaatctcg cgaccgatca tctggatctg ctgttaatcc accgaccaga cccgttaatg
     8521 gatgccgatg aagtggcgga cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat
     8581 tttggcgtat cgaactttac gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt
     8641 acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc agccgttact gctggatggc
     8701 acgctcgacc aactacaaca actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt
     8761 ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg atgaactggc tgtggtggcg
     8821 gaggagttaa acgcgggctc gattgaacag gtggtttacg cctgggtatt acgtttaccg
     8881 tcgcagccgc tgccaattat cggctcaggt aaaattgagc gcgtacgggc agctgtcgaa
     8941 gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta tccgtaaagc tgcactgggg
     9001 tacgacgtac cgtaagcact tttaggaata gccgccgttc aaaaatgtgt cactggttta
     9061 cacttattca ggaatgcaca atgaacggag gtcctatgaa acgttttagt ctggccattc
     9121 tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg
     9181 tcacgtcgca aggggttggg cagtcaattg gtagcgtcac cattactgaa accgataaag
     9241 gtctggagtt ttcacccgat ctgaaagcat taccccccgg tgaacatggc ttccatattc
     9301 atgccaaagg aagctgccag ccagccacca aagatggcaa agccagcgcc gcggaatccg
     9361 caggcgggca tcttgatcca caaaataccg gtaaacatga agggccagaa ggtaccgggc
     9421 atttaggcga tctgcctgca ctggtcgtca ataatgacgg caaagctacc gatgccgtca
     9481 tcgcgcctcg tctgaaatca ctggatgaaa tcaaagacaa ggcgctgatg gtccacgttg
     9541 gcggcgataa tatgtccgat caacctaaac cgctgggcgg tggcggtgaa cgctatgcct
     9601 gtggtgtaat taagtaatta cgaggccagc gtaccttgcg gtggtgcttg ctcaagttgc
     9661 gaaagcgagc agtacagccg ccagacaatt gccgccagct cacgggcggc aggttgatga
     9721 tgacgggcaa ggctgtcgca aatccgctgt aattcttcaa gtgtggccgc cagtgatttt
     9781 tgctgaacgc cacgctcact catcactccc cgcaacagtg aaatacagtt atcccgtact
     9841 cgcgataacg gatcggaacg cgattcccag tcgcgcaatt gccagacaac atgagaacag
     9901 ttcagcagca ctacacccca gcgtaataac caacggcgag ccagcgcatc ctggctgtta
     9961 ctcaactgac tgacgtgatg ataagtgagc gattcaaatt cgctttcact cagtgttgga
    10021 tggcggctta gctgatcgac aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta
    10081 cgagcatccg atcctggacg cagaatggcg aacgctagcc aagccagcgc aacgccaacg
    10141 atttttgcca gattatcgtt aagaaaatca gcaaaatcat acaccggggg attagtgaca
    10201 gcgataaacg aacccataaa aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc
    10261 tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga gaaacagtaa aaattgccac
    10321 agatcgctaa tctggaccat caggccaaat ttgaccacaa agctgaatag cgaaagtaac
    10381 accagcgtgc gcatcagcag tgacaacgac ttaaacggtg ctgcgacggt ggagtagagt
    10441 acgcagctaa ttgctgccag cgttaatgca ttggcaccgg catcccattg cgatgcaata
    10501 ctccatgcgc caatcatcat caacgtacaa aatgtacgca gcccgctcca catagcttcg
    10561 gcgttgtcgg tatgacgagc cagagcggat gtccgtgcga gtctggcatg atcatctaca
    10621 ccgctttgca gacgatgtaa ttcctgacta ctttgcagat aaaggcggca aaaataacgt
    10681 agtcgctgcc agaaggcgac gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg
    10741 ataatacgtg cgacggtgta agcatctgtt tgcgaactgg cgagcgccgt cagtaattgt
    10801 tcgagaattt ctcgcgtggc acctggcggt gagggccagt tgagcaacat acgtcgcagg
    10861 ctggagatga cactggtcat acgtaattgc tggtggagta gcgcattaag gcgcgcgttt
    10921 tgctggcgaa aacgatagtg gctccagaaa gcctggatac gcagcaaatt catggtcagt
    10981 atctgcccaa tcacctcctc atgtgctgca cgaatggcat cgtttgtttc aggctgccag
    11041 agtaaactgg catgttccag taatcgggcg tgcatgtttt tcaatgcagt taaaagggca
    11101 gtagcatcgg aactgctagg caggatcatc atcatcatgc cgccgcacaa aataccgact
    11161 atcacctcgc aaacgcgcgc ctgagcgata tcccacagct ggctggcctc agtaatatta
    11221 accatcggaa aggcgatgat ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg
    11281 ttattcgtga agtgcgcaca ggcctaggta caaaagccaa gccacgccga catgctcaat
    11341 agaaaaaacc acggctcatt gagcgtatgt ccggcaagaa gcagtgccgc aatggctccc
    11401 agtaaactgc cagcgatgcg tccgaggctt ttgctgatga caccgccaac ggtgggaaag
    11461 ctaaccactg cagccgaggt catcgcccaa tagggttcat ccagattcaa ataataggca
    11521 accgtcagcg ccagacacat ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc
    11581 gtggccctga accagcgcaa agcggacggg aacgcgttgg gcgagacgaa cccaggggac
    11641 gttaggtttc acatccggaa taaggcttga gtcactttca acgctctgat cataaatcgc
    11701 ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta tcactataga gggtaatttg
    11761 tgctggtgca ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta
    11821 aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat
    11881 gcgggtagtg aggttagtca cccatccact caccggggcg cgaatttccg tttgcgccag
    11941 ttgccattgt gtctgtttca gcgtcgcctg tgcggcatta acactggcct gcatcgcttt
    12001 aacattgagg ttagcggtat cgagttcttc ggcaaaaata aaattttgtg agagatgacg
    12061 acgacgattc gcttcgttat tggcttttgc gaggtcagat tgcgctttcg ccagctgagc
    12121 ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagttcccc
    12181 cgcgttaacc agttggttgt cttttatatt cagctcaaca atacgtcccg acacctgtgg
    12241 ggtgatagaa acctgttctg cgtgtatttt cccgtcgcgt gtccacggag attgcatgta
    12301 ataattccac agccaccatc cggcaagaac cgcaactatc gctacaatga ttgttgagaa
    12361 atacttaatt gttttcattg acatagttac cacgcaatca gtattgccag agcaaggcaa
    12421 acgcaaatcg caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag
    12481 atccagccac gaagcagacg gtgtacgaca agccagatga cgaatccaaa cgcgaatgct
    12541 ttgaaaaacg gaggaaaata gacggacgca ccgaacacca gatcttgtaa gggcaatcct
    12601 gttgcattga gcataaactt cacaaagtag attcctttac gaccgaatat attgcgttag
    12661 attaataaat attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga
    12721 cggtaaccaa atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa
    12781 aagccgcata atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg
    12841 ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc
    12901 gctggagtta acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga
    12961 ccagtcgcaa attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac
    13021 tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg
    13081 ggctaaacgt attaaactga cggaaaaggc agagccactg atcagcgaaa tggaagctgt
    13141 tattaacaaa acccgcgcgg aaatattaca tggcatctcc gcagaggaac tggagcaact
    13201 gattacgctc atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa
    13261 tgaagggggc gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggagaaacg
    13321 gtcacctgac tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg
    13381 ccttgtttct gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca
    13441 ccctgcgttt tgttcattgc actctgtacg ccctgaccag ctacgccacc tgcaacagcg
    13501 cctgctgcag tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca
    13561 ccaagaacag caccgccaat tgcaccgata acgttggaat catcaccgcc ctgaatctgt
    13621 accggacgta cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa
    13681 gcggtataaa catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct
    13741 accattgaaa caaccaatac gcgtttaatc actgaaaaat ctcctgttca ccatgaaacg
    13801 ccacgcaagc atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt
    13861 ctaccaaata tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc
    13921 atgatgatta ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg
    13981 catggtattc acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat
    14041 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
    14101 atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca gctggcaagc ttgagctggc
    14161 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac
    14221 tttcgcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg
    14281 ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc
    14341 aaaccgtctg gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca
    14401 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
    14461 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc
    14521 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
    14581 ctgggcagcc ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
    14641 tctggcgtca ggcaggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
    14701 ttattctccc actgctgcaa aatatgctca gcgacccgta tttctcgcaa cctgcaccga
    14761 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc
    14821 cgggtgttga tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt
    14881 ctgaacaagt tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc
    14941 gtaatccgct actcatggcg cgtctggcgg tattactgct aggcacagaa gtcaccacca
    15001 ccgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct
    15061 ggcggacgct ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
    15121 cggtactggg ggctattttc cccgccaact cgtgatatca gaattaactg aattttttct
    15181 tatcgtgtct gccgttacac ttggaagatt aaggagggta tttgccctcc ggaccaggaa
    15241 agtcttcggg atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc
    15301 ggttgtagcg cctttaatca actggttgag cgaatgcaaa ccgatacgct ggaataccag
    15361 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
    15421 tacgataatc aactactgca tctcaaacag ggcctttcag cctctggcgc gcgttactcc
    15481 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
    15541 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
    15601 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
    15661 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
    15721 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
    15781 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
    15841 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
    15901 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
    15961 ccttgaacgc caggttatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
    16021 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
    16081 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
    16141 tcaacagggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
    16201 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
    16261 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
    16321 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
    16381 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
    16441 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
    16501 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
    16561 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
    16621 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
    16681 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
    16741 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
    16801 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
    16861 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
    16921 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
    16981 ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
    17041 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
    17101 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
    17161 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
    17221 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
    17281 ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
    17341 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
    17401 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
    17461 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
    17521 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
    17581 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
    17641 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
    17701 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
    17761 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
    17821 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
    17881 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
    17941 cgaaattgtg caaggcattg ccattgccgc cattgataaa ttacacacct gtgatgccgt
    18001 attaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca
    18061 ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga
    18121 aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtacggcacg gtttgcctgc
    18181 cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa
    18241 taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt
    18301 gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt
    18361 caccgccgat gaagcctggc atatcagccg tccactggtg gattttggta tgcgccagcc
    18421 ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac
    18481 gctgcaggag gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa
    18541 agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga
    18601 acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg
    18661 gccgatggct attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca
    18721 gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg
    18781 tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag
    18841 atccagtgct catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg
    18901 cgaactgtcg gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta
    18961 tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt
    19021 accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg
    19081 ccttccgtca gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta
    19141 acggaaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc
    19201 gtaaaatcct ttccgctctc acgcagggta atatgggaag cgagagagca ggcgcccggt
    19261 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
    19321 gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa
    19381 ttttacgcta cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc
    19441 agtttaggcg cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc
    19501 aagaatacgc gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca
    19561 gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag
    19621 ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc
    19681 agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg
    19741 acagacacga tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca
    19801 ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc
    19861 ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc
    19921 tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca
    19981 aagaagttca gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc
    20041 atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct
    20101 ttaccgaaga taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc
    20161 tgattgtgca gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc
    20221 aacaggttac ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag
    20281 cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac
    20341 gcaacactcc agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag
    20401 ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc
    20461 tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca
    20521 atagccgcca tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca
    20581 taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg
    20641 tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa
    20701 agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt
    20761 ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac
    20821 gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg
    20881 gacactttta cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc
    20941 taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc
    21001 ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc
    21061 aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt
    21121 ttcactgcat tgagaggggg atgatggctt tacggagata tgttctgcta tttagcgttt
    21181 ttcctagtga gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata
    21241 tttgttgcgt gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc
    21301 cacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
    21361 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
    21421 attgacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
    21481 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
    21541 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
    21601 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
    21661 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
    21721 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
    21781 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc
    21841 cagtaatata cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt
    21901 tttgatatag gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
    21961 caccgggtag agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag
    22021 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
    22081 attgttctca cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc
    22141 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt
    22201 ctttcatcaa tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt
    22261 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
    22321 gcccagctac ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg
    22381 cgtagtgaac ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc
    22441 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
    22501 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
    22561 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
    22621 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
    22681 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
    22741 aatagcggac aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga
    22801 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
    22861 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
    22921 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
    22981 tgcgcatcat ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg
    23041 cgcaattcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
    23101 agtaccgtgc cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
    23161 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
    23221 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca
    23281 ttgttatagc gttcggctgg cagcacctga ccaaataacg ccttttgttg cagactggcc
    23341 tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc
    23401 gctgcaaata gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
    23461 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
    23521 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
    23581 actaataagc ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta
    23641 actgggtcag tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt
    23701 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
    23761 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg
    23821 ccaccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
    23881 tagatcggat attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat
    23941 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca
    24001 ccaccactgg cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg
    24061 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
    24121 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
    24181 tgtttagcga tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg
    24241 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
    24301 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc
    24361 gacgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
    24421 cctggcacgg ctgcgagcaa caccaatagc ataatgcgcg atgtctggcg ctggttatgg
    24481 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg
    24541 gcggcttttt tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg
    24601 acctgttctt ctggttccgc attagcctgt tgctgttcca gcttgcgcgc tttggagcgg
    24661 gcgatagctg cttcaacggc agttttacgt ggttcagcaa cggttgctgc gtcgttagtt
    24721 tgctccagtt ctgcctgttt cgctctggct tgcgctttac gggcttcccg tgctgcaata
    24781 attgcactgt tatccgggcg ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc
    24841 tgtttctctt tcacccgcgc cagagcggca gcaatcgcat ctttatcttt ggctgcaggt
    24901 tgaacggctg cgctcttatg tcgttcaagg cgagccgctt tttcgcgctc cagacgagcc
    24961 tggcgcgctt cgaaacgcgc tttggcttct gcggcgcgct tttcttcctg acgaatagcc
    25021 gcaatttcag ctttttcctg acggaaatat tgcaccaagg gaatattgct cgggcaaacc
    25081 cacgcgcaag ccccacattc aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt
    25141 tgctgacctt tgctgaacca gtacaactgt tgcggcaaaa gatccacagg gcaggcgtca
    25201 gcacaggcgc tacaccggat gcagctttgt tcttcctgtg gttcgccaag ttcattggca
    25261 gagggagcca acagacagtt ggtaatcttt acgaccggga catccagcca tggcaaggta
    25321 aagcccatta gcgggccacc cataatcacc atttgatcgg cagaggggca gaatccggca
    25381 tcattcaata aatgacgcac tggcgttccc agccgtgccc agacgttgcc cgggcgagcg
    25441 attgcttcgc cagtcagggt tacaacacgc tcggtaatcg gctcgccatc aataacggca
    25501 cgtttcactg cataagcagt gccgacgttt tgcattaata cgccgatatc ggatgaacgc
    25561 ccgccatgtg gaacctgctt cccggtcaga atgtaggtta attgtttagc accgccagaa
    25621 ggatatttgg ttggaatcac ccgcagagaa atatcgttag agtccgccag caccgcgcgc
    25681 agcatggaaa tcgcctgcgg tttgttatct tcaatgccga taagaatttc gcgtggctgc
    25741 agaatatgcg caagaatgcg aataccctct acgacctgag ccgcgcaatc ctgcatcaaa
    25801 cggtcatcgg cggtaatgta cggctcgcac tcagcagcgt tgataatcaa cgtttcaatc
    25861 ttatctccac caccctgcaa tttaacgcct gtcgggaatc cggcaccgcc cagcccggca
    25921 acaccaaact gatgtatgcg ctcgattaac tcttcgcgac tgcgagagcg ataatcgacc
    25981 cagccgtcgc gcgggatcca gcagtcttca ccatcggcat caataatcac gcttaattca
    26041 gctaaagctg aaggatgagc cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg
    26101 ggcgcgtgaa caggcagcat tttgccgcga ccacgggtaa gcggctggcc gcgcaatact
    26161 ttatcgccga cgctaacgca caactcacct tcagcgccaa tatgctgttt cagtggaata
    26221 acaaaacgct gcgccagtgg tacctggcgc aggggggtac cgttggactg ggttttcatc
    26281 tccggtggat ggatgccgcc gttgaaatcc cagattttat tttttctgaa tgcagagaat
    26341 aacttaagca tggtgttcca cgggaatgat acgcacggga atggtgttca gatcccattt
    26401 ccaggagtca ggtgtttctg cgaccggttg caacgagatg cagtgcgtcg ggcacggatc
    26461 aacacataaa ttgcagcccg tacagagatc actcattacc gtatgcatgg cacgggtagc
    26521 gccaacgatg gcgtctaccg gacaagcctg aatacattta gtgcagccaa tacagttatt
    26581 ttcatcaata accgccacca tccgcgcagg cgttagctct tgcgcttcgc catccagcgg
    26641 ctgcggctcg acattaagca actcggcaat ttttagcatc acagcttcgc cacctggggc
    26701 gcaacggttg attttttcac cgttacagct gatggcttcc gcgtaggggc gacagccggg
    26761 ataaccgcac tgaccacact ggctctgcgg taagatttcg tcaattttct caacgaccgg
    26821 atcgtcttcc accgcaaaac ggcgggaggc ataacccaga atggcgccaa acgccaggcc
    26881 cagcaggctc acggcggcaa cggcaatcca gatagcattc attacaactt caccaaacca
    26941 ctaaagccca taaaggccag agacataaga cctgcggtaa ttaacgcaat ggcattaccg
    27001 cgaaaaggtg ccgggacatc agccacagca aggcgttcgc ggatggtggc gaagagcacc
    27061 atcaccagcg agaaaccgac agcggcggaa aaaccgtaca gcgccgactg caagaaattg
    27121 tgcccgagat tgatattcag caacgccacg ccgagcactg cacagttggt ggtgataagc
    27181 ggcaaaaaaa tccccagcag acggtaaagc accgggctgg ttttgcgcac caccatctcg
    27241 gtgaactgca cgaccacagc aatcaccaga ataaatgcca gggtgcgtag gtaaatcaga
    27301 ttaagtggga tcaaaatcca cgtatcgata agccaggcgc aaatagacgt cagcgtcatc
    27361 acaaacgttg ttgccagccc catgcccatc gcggtttcca gttttttgga aacccccata
    27421 aacggacaga gaccgagaaa cttgaccagt acaaagttat tgaccagtac agttccgaca
    27481 aacagtaaca ggtagtcagt catttttcgg cctgaaataa aaaagccgcc tattatccgt
    27541 taatccagag caggcgacaa caggttaact gtaaggttat tacggattca caaacgtcgc
    27601 ttttacccgc gacgagcgtt tgaaataggg gacgatcaat gcggttgcca acaaagtaaa
    27661 cagcaactgg cgaacggcta tgccaacttc cacgggtgaa aaagcgaatg cttttactgc
    27721 cagcaatacg gaaataagca gccagataat atagtgttta ggaacacaac gccgacgttt
    27781 aaagaaagcg atggtcaacc acagtgtgta ataccacatg gcgatagcgg taataaaaga
    27841 aacgccccat aagatttgtg tcgtcagtgc ttgtccgccg agcgtttgaa atgtttgcgg
    27901 agaagataac gcagcggtat acagcaacaa cgccagcgtc gtactcaaca gagcgactaa
    27961 aagccaggcc aacgggccaa gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat
    28021 aggttctcca gaatagcgac gcaaacaggc aaatctaatt ttaggggcgt tattataagg
    28081 cttttataac cgatcgctac tttctttatt ggacatagtg ccagacggac ttcggtacct
    28141 ggccgaggtc aaacaaacgc ccgccagaaa ccagctctgc gcgacggtga tcggcagcac
    28201 gatacatatt gatcagttcc tgatcgtccg tcagggtgta attaagatgg tcgtagagtt
    28261 tttccagact ttcgagtgaa ctgattttgc gaaattttaa taagtagtcc tgaacagtca
    28321 taaaaggtcc ataaaataat taataccaaa ggggtaggta ttattagtga tgataaaatt
    28381 tcaccaaaag tgaacgcttg tgttattaca ataataagaa tgcgctgatg gcaaatattc
    28441 agggatgaaa aactgctctg aaaagagtaa cgcgctttac tatccctgac aatcaccaac
    28501 aacatcgaac aagataataa attcctggtt taatatccga caagtgaaaa catgcacccg
    28561 gacgggcagc atgtcgctcc acaagtgcag agcttacttg tgttgtaccg aagcactctg
    28621 ttcaggtggc tgatagttgt caatgtgact cgccacgcca agaagaatga ctgaaacgac
    28681 aagaacgatc caacctgtta attcaataag acgattcatt acagcccaca tctctcttga
    28741 ttgatccatt aacttcaggg ggtaaatgtt acttagcaat aatagctcca gcaagatttt
    28801 tactgaggtt tttgcgatat ttagcttttg tcgttggaaa attcgctatt tttttgactt
    28861 aagttaaaca acatccctta ttgctggcag gttattaaac tgttgagcgt gggtaaagga
    28921 tagtgtcaaa tagccatcat acttcaatga gaggcaatga catgagcgac aacatccgtg
    28981 ttgggttgat tgggtatggt tatgcgagca aaaccttcca tgcgcccctg attgcgggca
    29041 cgcccgggct ggaactggcg gtaatctcca gcagtgatga aacaaaagta aaagccgact
    29101 ggccaacggt tacggttgtc tctgagccga agcatctgtt taacgatccc aacatagacc
    29161 tgattgtcat tcctacaccc aacgataccc atttcccgtt agccaaagcg gcgcttgagg
    29221 cgggtaaaca tgtggtcgtt gataaaccct ttaccgtgac actgtcacaa gcgcgagagc
    29281 tggatgcgct ggcaaaaagc ctggggcgtg tgctgtctgt attccataac cgtcgttggg
    29341 atagcgattt cttgacgcta aaaggtttgc tcgcggaagg cgtgctgggt gaagttgctt
    29401 actttgagtc tcattttgac cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg
    29461 gcggcccagg cagcggtatc tggtacgatt tagcaccgca tcttcttgat caggccatta
    29521 cgctatttgg tttaccggtc agcatgacgg tagatttggc acagttacgg cccggagcgc
    29581 agtcgaccga ttatttccac gccatcttgt cctatccaca gcggcgagtc attttacacg
    29641 gtaccatgct ggcagccgct gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt
    29701 atgtgaaata tggcctcgat ccacaggaag aacgtctgaa aaatggcgag cgtctgccgc
    29761 aggaagactg gggctacgat atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac
    29821 gtgtcgaaga aacgctgttg acggtgcctg ggaattatcc ggcttactat gcggcgattc
    29881 gtgatgcgtt aaatggcgat ggtgaaaatc cggttccggc aagccaggca atccaggtaa
    29941 tggagttgat tgagctgggc atcgaatccg ccaaacatcg cgcgacgttg tgccttgcgt
    30001 gaaaaatgga tggtgcaatt tgcaccatcc atttttgtta cttcgcggcg actttttctc
    30061 gcagtgcgcg tttttcctct gcgctgagga aagccatttc cagaccatta atctgtgcct
    30121 ggcggatttg ctcacgggat aacccagcag ctggcgcggc aacggtatat tcgtgaatga
    30181 tatccactcc ctgtacgccg ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa
    30241 ggaacgtttt cagcggatgt gcagccagct ctgctacggt gctggtctga atattggagg
    30301 tcagacagga ttcaatacca atttgttgct cggcgagaaa atccatcagc gcccgatctt
    30361 caatggcttt tacgccatgt ccaatacgct ccgcacccag ttcacgaatc gcctgccaga
    30421 tgctttccgg cccggcagct tcgcctgcat ggacggtaat atgccagccc gcatcacgcg
    30481 cgcggttgaa gtgagaaagg aacagacttc ccgggaatcc aagttcatca ccagctaaat
    30541 caagtgcggt aatctggtca cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg
    30601 cttcgccgaa ggttcggctc ataatgccga taagcttcgc ctgcacacca aaggtgcggc
    30661 aaccttcacg tacgccatcg atcaccgctt cgacaacacc cgctacaggc agcttatgtg
    30721 ccattgccat gtagcctggt gaaaaacgca gctcgacata gcgcaggccg ttacgggctg
    30781 catcttcaat gttttcaaat gccacgcggc ggcaggcatc aagagaggcg agaactttaa
    30841 cgccccagtc aagtttggtc agaaagctca ccagatcggg ttcgttggca atgacctgaa
    30901 cgtggggaat cagtgtttca agggattgtg caggaagcga gatattatac tggcggccaa
    30961 gttcaagaat ggtctgggga cgaatgttgc catcaaggtg gcgatggata tcagttaatg
    31021 gcagggtggt atcaatcatg gtcgcactct ttttctggat ataaaagtgc gcaccattat
    31081 aaaaacaaag cagggtaaaa atccacgatt gcgcaacgat ttttacgttg cgcaattggg
    31141 gttagcgaac agcgcggatg gcgtttatta atccagccac acctttttcc agtttcgaac
    31201 gtgggcagcc ggcattgaga cggacaaaac cacgaccttc ttcaccatag gtataccccg
    31261 gcatgatcgc gactttttct tgctcgataa gcgctttttg caacgcgttg tcgtcaatat
    31321 tcaacggacg tagatcaagc caggccagat aagtggattg cgggatctgc cagttgagtt
    31381 caggaaacgc ggcgttcatt ttatctgaga tatacgtcag gttatctttc agatagacgc
    31441 gtaaggcatc cagccacggt gcaccttgct gataggcggc gatatgggca gttaacgcca
    31501 gtaccgaagg ggaagaaagc ccatcacgtc ctttcagtgc cgataaatag gcatcacggc
    31561 tgctgctatt ttctataatc ccgtaagcac cggtcagcgc gggaatattg aaacttttcg
    31621 atcccgacgt cagcaacgcc cagtctcccc gcgccacatt actccaggga atatgcggct
    31681 gctcgcccca gaccatatcc atatggattt catcggaaat aacccgcaca tcatgacgct
    31741 cgcacaggtc agccatgatc tccagctcat cgcacgtcca cactttcccg gtaggattct
    31801 gtgggttaca caggagcata attttacatt ctggtttcgc caaaacggct tccagcttgc
    31861 ccatatcgca aaaccagccg tcagcctgct tctctaaagc aacgggcatt actgtgcgct
    31921 ggttaccttc aatggccttg taaaatgcgt cataggcggg cgtgtggatc accacgcctt
    31981 cacctgtttc agaccactga cgaatcagtt ctgaaaccat atagatgaca gaagggccat
    32041 acaccaccgt ctgcgtatca attgcggtgt aatgctgggt ggaaaaccag tgggcaatag
    32101 ccgcgagaaa ctcatcgttt ttccagcggc tgtagccaaa tacgccgtgc atcaggcgct
    32161 gattcagcgc ctcgataatg cagggggcag tggcaaaatc catgtctgaa atcgtgaacg
    32221 gtaacaggtc agcagtgccg aaacggtcag cgacataatc ccactgtgta caccatgtgc
    32281 catgacgatc cacgaccttt gaaaaatcga acatatctta tccttatgcc tggacagtat
    32341 gcatcagacc ggccatttca tcttttactg actgaacttg tggcccgata acaacctgca
    32401 ggttatgttg attaagttgt actacgccaa ttgcccgatt gtccttcagt gcttgcacat
    32461 taacaagcga catatcttcc acagacaaac gcagacgggt aatgcagtta tcgaggctga
    32521 caatattgtc ggcaccgcct aatgcttcga ggattgcagg aacgttgtaa cctgatttac
    32581 ccggcgcacc ggcaacggct ttttcgattg agctggcaac ttcgctatcg cgccccgggg
    32641 ttttcagatt gaagcgggtg atagcgaaac ggaagatgac gtagtaaacg acaaaccaga
    32701 ttgccgccac cactggcacc atgtaccact tggttgacag accatgcaaa ataccgaaca
    32761 ccacgaagtc gatgatattg ccgtcggtat taccgatggt gacgccgagc acagacatga
    32821 cggtgaagcc gaggccggtt aacagcgcgt ggatgacata cagaactggc gctacgaaca
    32881 ggaacaggaa ttccagcggt tcggtagtgc cgccaacgac gcaggcgatc aggccagaaa
    32941 tcagcagacc tttaatttta tggcgatttt ccgggcgcgc gcagtgatac atagctaacg
    33001 ctgcacctgg cagaccgccg agaaacgcag gcattttacc ttgcgaaagg aaacgcgtga
    33061 cgctttcaga aaaaccgtga gtggtcgggc aactcaattg cgcctggaag atggtcagtg
    33121 cgccgctgac ggtttgaccg cagacctcct gcgtgccgcc tgcgtcggta aagcgaatta
    33181 atgccaccag aatgtgatgc agaccaaacg gtaacagcag acgttcaccg gtaccaaaca
    33241 gcatcggtcc gaaatcaccc gcgctgttta tcatatggcc caagccgcta atacccatgg
    33301 cgaaaatcgg ccagactaat ggaatcacca ggccgacaag gcccatcacc agcgaggaga
    33361 taattggtac gaagcgcgta ccgccgaaga atgccagcgc atccggcagg cggatattat
    33421 ggaaacgctc atgcagcatc cagacgataa taccggcgat caccgcaccg aggatcccgg
    33481 tatcgatcga ctggatccca agaatgctct ggatgttatt ggctttcaga acggcggcat
    33541 ctgtggttgg cagaatgcct ttattggtca accagaagtt taccgcgagg ttcattaccg
    33601 cataaccgac gaagccagcg aatgccgcta cgcctttatt ttcgcgtgcc aggcccagcg
    33661 ggatggcgat acagaacatg acaggcagga aactaaaagc aaacgagcca atcttactca
    33721 tccaggtaaa gatagcctgc aacacggggt tgcccaggac cgggatcagg gttatgacat
    33781 catggctgct aagagaacta ccaatgccga gcataatgcc gcagaacgac aataatgcca
    33841 cgggtaacat gaaggttttg cctaactgct ggaagaactc ccacagcgtg actttcggtg
    33901 ctgttttcgc cgtcataaaa cgactcctcg tagagaacaa tctggattca gtaaattgcg
    33961 cgagaagata aaacgtttta tcaaatttta gtgagtcata aatcacatta cgcaacgata
    34021 atagcgggta taagataaat aaaaggtaaa acgttttatc tgtcacataa tcagggagta
    34081 ggtcatctgc atggctaccg ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg
    34141 cgtgtcggta agtaccgttt cgctggtgct tagtggcaaa gggcgaatct ctaccgccac
    34201 aggagaacgc gttaacgccg ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc
    34261 ggcgctgcgc ggcgggcaaa gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc
    34321 gttttacgcc gaattgacgg ccggattgac ggaagctctg gaagcgcagg gacggatggt
    34381 ttttttgctt cacggcggta aagacggcga gcagctggca cagcggtttt cactgttact
    34441 gaatcagggt gtcgatggtg tggtaattgc cggggctgca ggaagcagcg atgacctgcg
    34501 gcggatggca gaagaaaaag ctatcccggt gattttcgct tcccgtgcca gttatcttga
    34561 tgatgttgat acggttcgcc cggacaacat gcaggctgca cagttgttga cggagcatct
    34621 cattcgcaat gggcatcagc ggatcgcctg gctgggaggg caaagttcct cattaacccg
    34681 tgcagaacgg gtggggggct attgtgcaac tctactaaaa tttggcctgc cgtttcacag
    34741 cgattgggtg ttggagtgca cttccagcca gaagcaagcc gcggaagcta tcacggcgct
    34801 tttacgtcat aacccgacca tcagtgccgt ggtttgctat aacgaaacta ttgcgatggg
    34861 ggcatggttt ggtttgctga aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta
    34921 ctttgagcaa caggtttctc tggcggcatt taccgatgcg acaccaacca cacttgatga
    34981 tatacctgtt acctgggcca gcacgccagc gcgggaactt ggtatcacac ttgcggatcg
    35041 catgatgcaa aaaatcaccc atgaagagac gcattcacgc aatcttatta ttcccgcccg
    35101 gctcattgcg gcgaaataat cctctcttta tctgctatac ttggtagtgt cctttcctca
    35161 aggttaatga taaaaaatgt ggcacacagg cggtgccttt tatttttact gaagttcaca
    35221 ggaggtttat gtgtttaatt ctgacaacct gagactcgac ggaaaatgcg ccatcatcac
    35281 aggtgcgggt gcaggtattg gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc
    35341 tgtggtggtc agtgatatta acgccgacgc agctaaccat gttgtagacg aaattcaaca
    35401 actgggtggt caggcatttt cctggcgttg tgatattact tccgaacagg aactctctgc
    35461 actggcagac tttgccgtca gtaaactggg taaagttgat atcctggtta acaacgtcgg
    35521 tggcggtggt cctaaaccgt ttgatatgcc aatggcagat tttcgccgtg cttatgaact
    35581 gaatgtgttt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg
    35641 cggtggcgtt attctgacca tcacttctat ggcggcagaa aataaaaata taaacatgac
    35701 ttcctatgca tcatctaaag ctgcggccag tcatctggtc agaaatatgg catttgacct
    35761 gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg gcaatattaa ccgatgccct
    35821 gaaatccgtt attacaccag aa
//
LOCUS       DADLTI010000020        35573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLTI010000020 DADLTI010000000
VERSION     DADLTI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.020"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1046)
                     /locus_tag="JN755_000926"
     CDS             complement(<1..1046)
                     /locus_tag="JN755_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbD family protein"
                     /protein_id="HAY7379809.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAA"
     gene            1471..2166
                     /gene="aqpZ"
                     /locus_tag="JN755_000927"
     CDS             1471..2166
                     /gene="aqpZ"
                     /locus_tag="JN755_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7379810.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            2623..3522
                     /gene="lysO"
                     /locus_tag="JN755_000928"
     CDS             2623..3522
                     /gene="lysO"
                     /locus_tag="JN755_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7379811.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            3666..5318
                     /gene="hcp"
                     /locus_tag="JN755_000929"
                     /gene_synonym="priS"
     CDS             3666..5318
                     /gene="hcp"
                     /locus_tag="JN755_000929"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7379812.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            5330..6298
                     /gene="hcr"
                     /locus_tag="JN755_000930"
     CDS             5330..6298
                     /gene="hcr"
                     /locus_tag="JN755_000930"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7379813.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            6431..8149
                     /gene="poxB"
                     /locus_tag="JN755_000931"
     CDS             6431..8149
                     /gene="poxB"
                     /locus_tag="JN755_000931"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7379814.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            8186..9187
                     /gene="ltaE"
                     /locus_tag="JN755_000932"
     CDS             8186..9187
                     /gene="ltaE"
                     /locus_tag="JN755_000932"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7379815.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            9198..10628
                     /locus_tag="JN755_000933"
     CDS             9198..10628
                     /locus_tag="JN755_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7379816.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            10727..11740
                     /locus_tag="JN755_000934"
     CDS             10727..11740
                     /locus_tag="JN755_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7379817.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(11737..12567)
                     /locus_tag="JN755_000935"
     CDS             complement(11737..12567)
                     /locus_tag="JN755_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY7379818.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(12564..12887)
                     /locus_tag="JN755_000936"
     CDS             complement(12564..12887)
                     /locus_tag="JN755_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7379819.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(13098..14867)
                     /locus_tag="JN755_000937"
     CDS             complement(13098..14867)
                     /locus_tag="JN755_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7379820.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            15516..16031
                     /locus_tag="JN755_000938"
     CDS             15516..16031
                     /locus_tag="JN755_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7379821.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            16249..16977
                     /gene="artP"
                     /locus_tag="JN755_000939"
     CDS             16249..16977
                     /gene="artP"
                     /locus_tag="JN755_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7379822.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            16995..17726
                     /gene="artJ"
                     /locus_tag="JN755_000940"
     CDS             16995..17726
                     /gene="artJ"
                     /locus_tag="JN755_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7379823.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            17733..18449
                     /gene="artQ"
                     /locus_tag="JN755_000941"
     CDS             17733..18449
                     /gene="artQ"
                     /locus_tag="JN755_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7379824.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            18449..19117
                     /gene="artM"
                     /locus_tag="JN755_000942"
     CDS             18449..19117
                     /gene="artM"
                     /locus_tag="JN755_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7379825.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            19409..20140
                     /gene="artJ"
                     /locus_tag="JN755_000943"
     CDS             19409..20140
                     /gene="artJ"
                     /locus_tag="JN755_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7379826.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20315..21442)
                     /gene="rlmC"
                     /locus_tag="JN755_000944"
     CDS             complement(20315..21442)
                     /gene="rlmC"
                     /locus_tag="JN755_000944"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7379827.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(21483..21971)
                     /locus_tag="JN755_000945"
     CDS             complement(21483..21971)
                     /locus_tag="JN755_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7379828.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(22031..22876)
                     /gene="potI"
                     /locus_tag="JN755_000946"
     CDS             complement(22031..22876)
                     /gene="potI"
                     /locus_tag="JN755_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7379829.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(22873..23826)
                     /gene="potH"
                     /locus_tag="JN755_000947"
     CDS             complement(22873..23826)
                     /gene="potH"
                     /locus_tag="JN755_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7379830.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(23893..24969)
                     /gene="potG"
                     /locus_tag="JN755_000948"
     CDS             complement(23893..24969)
                     /gene="potG"
                     /locus_tag="JN755_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7379831.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            complement(25064..26176)
                     /gene="potF"
                     /locus_tag="JN755_000949"
     CDS             complement(25064..26176)
                     /gene="potF"
                     /locus_tag="JN755_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7379832.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYIDDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(26528..27004)
                     /locus_tag="JN755_000950"
     CDS             complement(26528..27004)
                     /locus_tag="JN755_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7379833.1"
                     /translation="MTSLVVPGLDTLRQWLDVLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(27092..27994)
                     /gene="rimK"
                     /locus_tag="JN755_000951"
     CDS             complement(27092..27994)
                     /gene="rimK"
                     /locus_tag="JN755_000951"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7379834.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(28055..28777)
                     /gene="nfsA"
                     /locus_tag="JN755_000952"
     CDS             complement(28055..28777)
                     /gene="nfsA"
                     /locus_tag="JN755_000952"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7379835.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(28761..29048)
                     /locus_tag="JN755_000953"
     CDS             complement(28761..29048)
                     /locus_tag="JN755_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7379836.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            29208..29465
                     /gene="grxA"
                     /locus_tag="JN755_000954"
     CDS             29208..29465
                     /gene="grxA"
                     /locus_tag="JN755_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7379837.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(29495..29872)
                     /locus_tag="JN755_000955"
     CDS             complement(29495..29872)
                     /locus_tag="JN755_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379838.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            30142..31827
                     /locus_tag="JN755_000956"
     CDS             30142..31827
                     /locus_tag="JN755_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7379839.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            complement(32002..32538)
                     /locus_tag="JN755_000957"
     CDS             complement(32002..32538)
                     /locus_tag="JN755_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7379840.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            32622..33830
                     /locus_tag="JN755_000958"
     CDS             32622..33830
                     /locus_tag="JN755_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379841.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            33830..34645
                     /gene="ybjI"
                     /locus_tag="JN755_000959"
     CDS             33830..34645
                     /gene="ybjI"
                     /locus_tag="JN755_000959"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7379842.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            34731..35016
                     /locus_tag="JN755_000960"
                     /pseudo
     CDS             34731..35016
                     /locus_tag="JN755_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35057..>35573)
                     /gene="mdfA"
                     /locus_tag="JN755_000961"
     CDS             complement(35057..>35573)
                     /gene="mdfA"
                     /locus_tag="JN755_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7379843.1"
                     /translation="AQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRT
                     VRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFA
                     SDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFL
                     KDKQMGNSHEG"
BASE COUNT         8765 a         9097 c         8832 g         8879 t
ORIGIN      
        1 caggcggcaa cacgagaaga ttcacgcacc aggcggcaaa catgttccac tggagttaac
       61 gaaagcagct cgccagagtt ggtggtagca atacgctgta aaggcaaaag attcaacaga
      121 tgccaggcaa cggaaagcat aatcggatgc aggcgggttt ctggatcttc aattagcaat
      181 aacggacgcg cgtctttatc cagtcgcaat gtgccttttg cctgcaataa agtagcaaac
      241 aggccgagca aaataacccg atacgagcgc cctccaggtc ggtcaatcat ccggttgatg
      301 atgtccagat agcgccagct tcgttgctca ttgctggccc gccgccgcat taaacgatat
      361 cgcgcctgtc cagccccctg ctcagagaaa taatgctcaa gcaactgcac cattgcggaa
      421 agtccctgac gaatctgtcc atcagagaga ttttgcggat gtgaggacaa ctcacgggcg
      481 aggaaatcga gctggcgcgc ggtgacttcc acattgggga catttggcac cgtgccgtta
      541 cgaatacggc gcataaaacg ggcatcacgc aagcgcagca ccggcattaa acgcaccaga
      601 tggcgtgcct gatcgttaat atcctcaaca tcaatcggat gtccgtcttt atcgagaaaa
      661 ctgcgcagtg tcatcacgct gccgtcttcc gcactctccc cttccagacg ataaaaaata
      721 cggtgatagc catcggtgca tggcgtccag cacgcttcca gcggccgata acggcgaacc
      781 cgatgtcggc ctggcagcga ttcgcggaag gtcaaaataa tatgcagatg atgttctcgc
      841 ccgttgatat ctcccggcgg gaaccagaaa tcgtcgcgct caaaatggta gagatctgat
      901 tctggcgata gcagcagggt taaggcgtcc agcaagctgg atttacccca cgcgttctcc
      961 ccaatcagga cgttgttttg ttcgagcatc aacgacaaac ggttgatacc gcgaaaaccc
     1021 acaatttcaa cgcgctcaag aatcatatcc cctccagaaa tatgtgactt atcctttacg
     1081 ctggcagtat aacgggaaag gccttgctaa tacgtgatga catcacattt aagcatggca
     1141 attgtctgaa aaatgagcga ataatcgcgt tatactccct cttgctgata agcccgcaaa
     1201 aacaacaaaa tcaaataatt agcatgataa attatttttt attacgccta aaggattaaa
     1261 tcttttgctt gcactacact gtcaatcaga caaatgacta tatggggcaa cccgctgttg
     1321 ccctaaatca aatttactgg atttatttat ctggtgaatg actcgccttc gtttttaaaa
     1381 tagccgcgca ttatttccgt cgtcattttt tacgacgacc ctataaaacg accctatttt
     1441 tcacagggtc aatttttaat tgaggtggat atgttcagaa aattagcagc tgaatgtttt
     1501 ggtactttct ggcttgtttt tggtggctgt ggtagtgctg tactggccgc aggcttcccg
     1561 gaattaggca ttggttttgc cggcgtggcg ctggcatttg gtttgaccgt tctgacgatg
     1621 gccttcgctg ttggtcatat ttctggtggt cattttaacc cggcggtcac tattggttta
     1681 tgggctggcg gacgttttcc ggcaaaagaa gtcgttggct acgtgattgc ccaggttgtc
     1741 ggcggtattg ttgcagcggc gctgctgtat ttaattgtca gtggtaaaac gggttttgac
     1801 gcggcagcca gcggttttgc ttctaacggt tatggcgagc attcaccagg cggttattcc
     1861 atgctttccg cgctggtagt tgaactggta ttgagtgcgg gcttcctgtt agtgatccac
     1921 ggtgcaaccg acaaattcgc tccggcaggt tttgcgccga tcgctattgg tctggcctta
     1981 accctgattc acttaatcag tattccggtg actaacactt ctgttaaccc ggcgcgcagc
     2041 accgcggttg ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg
     2101 gtgccaattg tcggcggcat tatcggtggt ctgatttacc ggactctgct ggaaaagcgt
     2161 gattaattag cattggcatt ttatctgccc tggttgtttt atcttcgaca ttacaaaccg
     2221 cacgggtatt ggtggcgtca aaatcgctga agcgttcccc gaaggccggg gcagcccaca
     2281 tggatgtggg ctgagggcgc gtcttacagg acgttacctc gcgcccgacc tggttgccga
     2341 aggggataag ctgaaggtac cgcgcagcgg cgattttgtt cgccagagcc cgggggtgca
     2401 gggggcggcg gcgactggct gccccctgcg cgctccctcc gccagtggct ttatatggca
     2461 ttgcacatga aaggagcgca acaatgctaa acactcagat aggattaaaa atccccctct
     2521 gatgcataac atttcctttt aattgcaact ttactcgtcc ttccgctttc agtagtgtgt
     2581 catcgcgcat ttcgacttct tctttgcaag gactggattt tcatgttttc tgggctgtta
     2641 atcattctgg ttcccctgat tgtgggttac ctcattccgc ttcgccaaca agctgcgtta
     2701 aaagttatta atcagctatt aagctggatg gtttacctta ttctcttttt tatgggtatc
     2761 agtctggcgt ttctcgataa cctcgccagt aacctgttgg cgattctgca ttattctgcc
     2821 gtcagtatta ccgttatttt actgtgtaat attgccgccc tgatgtggct ggagcgaggc
     2881 ctgccgtggc gcaaccacca tcagcaagaa aaactcccgt cgcgtattgc gatggcgctg
     2941 gagtcgctaa aactgtgcgg cgtagtagtg attggttttg ccattggtct gagtggactg
     3001 gctttcttac aacacgcgac cgaagccagt gaatacacgt taattttgct acttttcctc
     3061 gttggtattc agttgcgcaa taatggcatg accttaaagc agattgtcct taatcgccgg
     3121 ggaatgattg tcgccgtggt ggtggttgtc agttcattaa ttggtggttt aattaacgcc
     3181 tttattcttg atctccccat caataccgcg ctggcaatgg cctccggttt cggctggtat
     3241 tctctttccg gtattttatt gaccgaatct tttggtccgg taatcgggag cgcggcgttt
     3301 tttaatgatc tggcccgtga actgattgct attatgttga tccctgggct gattcgccgc
     3361 agccgctcta ctgcactggg cttatgcggt gccacatcaa tggatttcac cctgcccgtt
     3421 cttcaacgta ctggcgggct ggatatggtc ccggcggcaa ttgttcacgg ttttattctt
     3481 agcctgttag tgccgatcct catcgccttt ttctctgcgt aatacctctc tggcggtaga
     3541 tccctgccgc caaaattgcg ctaaatcaat ctcccttaaa gttgcatgaa aaatcccttt
     3601 tatccccgcg ttaagcgtct taaccttaaa catgtatatt aaatataact ttaaaaggtg
     3661 tgatcatgtt ttgtgtgcaa tgtgaacaaa ctatccgtac tccggcagga aacggctgct
     3721 catacgcgca ggggatgtgt ggtaaaacgg cggaaacttc tgaccttcag gatttactca
     3781 tcgcggcgct gcaagggctt tctgcctggg cggtaaaagc gcgtgaatac ggcatcatca
     3841 accacgatgt tgacagcttc gcgccacgtg cctttttctc aaccctgacc aacgttaact
     3901 tcgattctcc gcgtattgtc ggctacgctc gtgaagcgat tgccttgcgc gaggcgctga
     3961 aagcacaatg cctggctgta gatgccaacg cgcgcgtcga taacccgatg gctgacctgc
     4021 aactggtgag cgatgatctc ggcgagctgc aacgtcaggc agcagaattt acccctaaca
     4081 aagataaagc ggcgattggc gaaaacattc tcggcctgcg tctgctgtgc ctgtatggcc
     4141 tgaaaggtgc ggcggcctat atggaacacg cgcatgttct cggtcaatac gacaacgata
     4201 tttatgccca gtaccataaa atcatggcgt ggctggggac ctggcctgcc gatatgaacg
     4261 cgcttcttga gtgttcaatg gaaatcggcc agatgaactt caaagtgatg agcattctgg
     4321 atgcaggcga aaccggtaaa tacggtcacc cgacgccaac ccaggtcaac gtcaaagcga
     4381 cggcgggtaa atgcattctg atttccggtc acgatctcaa agatctctac aacctgctgg
     4441 aacagaccga aggcacgggc gttaatgtct acacccacgg cgaaatgctg cctgcgcatg
     4501 gctacccgga gctgcgtaaa ttcaagcatc tggtcggtaa ctacggcagc ggctggcaga
     4561 atcagcaagt ggagttcgct cgtttccctg gccccatcgt gatgacctcg aactgcatca
     4621 tcgacccaac cgtaggcgct tatgacgatc gtatctggac ccgcagcatc gttggctggc
     4681 ctggcgtgcg tcatctggat ggcgaagatt tctctgcggt catcgcccag gcgcaacaaa
     4741 tggcgggctt cccgtacagc gaaattcctc acctgatcac cgtgggcttt ggtcgccaga
     4801 cgctgcttgg tgcagccgat acgctgattg atctggttag ccgtgaaaaa ctgcgtcata
     4861 tcttcctgct tggtggctgt gacggcgcac gcggcgagcg ccactacttc accgatttcg
     4921 ccaccagcgt gccggatgac tgcctgatcc tgaccctcgc ctgtggtaaa tatcgcttta
     4981 acaaacttga gtttggcgat atcgaaggtc tgccgcgtct ggtagatgca ggtcaatgta
     5041 acgatgctta ctcagcaatt attctggctg tcactctggc agagaaactg ggctgtggtg
     5101 tgaacgatct gccgctgtcg ctggtgctct cctggtttga acagaaagcg attgtcattc
     5161 tgctgacgct gctttctctg ggcgtgaaaa atatcgtcac cggcccgact gcacctagtt
     5221 tcctgacacc agacctgctg gcggtgctga acgagaaatt cggcctgcgt tctatcacca
     5281 ctgttgaaga agacatgaag caactgttga gcgcgtaagg agtttgtcga tgacgatgcc
     5341 aacgaatcaa tgcccgtggc ggatgcaggt tcatcacatt acgcaagaaa cgccggatgt
     5401 gtggacgatt tccctgattt gccacgatca ctacccatat cgcgccgggc aatatgcact
     5461 ggtcagcgtg cgaaactcag cggaaacgct gcgtgcttac accatttcct ccacgccagg
     5521 cgtgagtgaa tatatcaccc tgaccgtgcg gcggattgat gacggtgtcg gctcccagtg
     5581 gctgacgcgc gatgtaaaac gcggtgatta tctctggctt tcggacgcga tgggggaatt
     5641 tacctgcgac gataaagcag aagataaatt cctgttgctg gcggcaggct gcggcgtcac
     5701 gccgattatg tcgatgcgtc gctggcttgc gaagaaccgt ccacaggccg atgtgcgggt
     5761 gatctacaac gtgcgtacgc cgcaggatgt tattttcgcc gatgagtggc gtaactatcc
     5821 ggtaacgctg gtggcagaaa ataacgttac cgaaggcttt atcgctggtc gtctcactcg
     5881 cgaactgctg gcagatgtac ctgacttagc ttcacgtacc gtgatgacct gcggcccggc
     5941 tccgtatatg gattgggtag agcaggaagt gaaagcgctc ggcgtgacgc gtttctttaa
     6001 agagaaattc ttcaccccag tagcagaagc ggcgaccagc ggtctgaaat tcaccaaact
     6061 gcaaccggca cgagaatttt acgccccggt tggcaccacg ctactggagg cgctggaaag
     6121 caataacgtt ccggttgtcg ctgcctgccg tgcgggtgtt tgcggctgct gtaagacaaa
     6181 agtggtttcc ggtgaatata cggtgagcag tacaatgacg ctgaccgacg ccgaaatcgc
     6241 tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc tcgcataatc
     6301 gccttatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc ctcctttctc
     6361 tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca cattcaggag
     6421 atggagaacc atgaaacaaa cggttgcagc ttatatcgcc aaaacactcg aatcggcagg
     6481 cgtgaaacgc atctggggag tcacgggcga ctctctgaac ggtcttagcg acagtcttaa
     6541 tcgcatgggc accatcgagt ggatgtccac ccgccatgaa gaagtggcgg cgtttgccgc
     6601 tggcgctgaa gcacaacttt caggagaact ggcggtctgt gccggatcgt gcggcccagg
     6661 caacctgcac ttgatcaacg gcctattcga ttgccaccgc aataacgttc cggtactggc
     6721 gattgccgct catattccct ccagcgaaat tggcagcggc tatttccagg aaacccaccc
     6781 acaagagcta ttccgcgaat gtagtcacta ttgcgagctg gtttccagcc cggagcagat
     6841 cccacaagtg ctggcaattg ctatgcgcaa agcggtgctt aaccgtggcg tttccgttgt
     6901 tgtgttaccg ggcgacgtgg cgttaaaacc tgcgccagaa ggggcaacca cgcactggta
     6961 tcatgcgcca cagccagtcg tgacgccgga agaagaagag ttacgcaaac tggcgcaact
     7021 gctgcgttat tccagcaata tcgccctgat gtgtggcagc ggctgcgcgg gggcgcataa
     7081 agagttagtt gagtttgccg ggaaaattaa agcacctatt gttcatgccc tacgcggtaa
     7141 agagcatgtc gaatacgata atccgtatga tgttggaatg accgggttaa tcggattctc
     7201 gtcaggtttc cataccatga tgaacgccga cacgttagtg ctactcggta ctcagttccc
     7261 ctaccgtgcg ttctacccga ccgatgccaa aattattcag attgatatta atccagccag
     7321 catcggcgca catagcaagg tagatatggc gctggtcggc gatatcaaat caaccctgcg
     7381 cgcattgctg ccactggtgg aagaaaaaac cgatcgcaag tttctggata aagcgctgga
     7441 agattaccgc gatgcccgca aagggctgga cgatttagct aaaccgagcg agaaagccat
     7501 tcacccgcaa tatctggcgc agcaaattag tcattttgcc gccgatgacg ccatctttac
     7561 ctgtgacgtc ggcacgccaa cggtatgggc ggcacgttat ctgaaaatga acggcaagcg
     7621 tcgcctgtta ggttcgttta accacggttc gatggctaac gccatgccgc aggcgttggg
     7681 tgcgcaggca accgagccgg aacgtcaggt ggtcgccatg tgcggcgatg gcggttttag
     7741 catgttgatg ggcgatttcc tctcagtagt gcagatgaaa ctgccggtga aaattatcgt
     7801 ctttaataac agcgtgctgg gctttgtggc gatggagatg aaggccggag gttacctgac
     7861 agacggtact gagctgcacg acaccaactt tgcccgcatt gccgaagcgt gcggcattac
     7921 gggtatccgt gtagaaaaag cctctgaagt tgatgaagcc ctgcaacgcg ccttctctat
     7981 cgacggtccg gtgttggtgg atgtggtggt cgccaaagaa gaattagcca ttccaccgca
     8041 gatcaaactc gaacaggcca aaggttttag cctgtatatg ttgcgcgcaa ttatcagcgg
     8101 acgcggcgat gaagtgatcg aactggcgaa aacgaactgg ctaaggtaaa aagggtggca
     8161 tttcccgtca taataaggac atgccatgat tgatttacgc agtgataccg ttacccgacc
     8221 gagccgcgcc atgctcgaag cgatgatggc cgccccggtt ggggacgacg tttacggaga
     8281 cgaccctacc gttaatgctc tgcaggacta tgcagcagag ctttccggta aagaagccgc
     8341 catttttctg cctaccggca ctcaggccaa cctggtcgct ctgctcagtc actgcgaacg
     8401 tggcgaagag tatattgtcg gtcaggccgc gcataactat ctgtttgaag ccggtggcgc
     8461 agcggtgctg ggcagtattc agccgcaacc catcgacgcg gctgccgacg gcacgctacc
     8521 gctggataaa gtggcgatga aaatcaaacc cgacgatatc catttcgccc gcaccaaatt
     8581 actcagtctg gaaaacaccc acaacggcaa agtgctgccg cgtgaatacc tgaaagaagc
     8641 atgggaattt acccgcgagc gcaatctggc gctgcatgtt gacggtgcgc acatctttaa
     8701 tgccgtggtg gcttacggct gcgaactgaa agagatcacg caatattgtg attcgttcac
     8761 catttgcctg tcgaaaggtc ttgggacgcc agtcggttca ttactcgtcg gtaatcgtga
     8821 ttacattaaa cgtgccatcc gctggcggaa aatggccggt ggcgggatgc gtcagtccgg
     8881 gattctggct gccgccggga tgtatgccct gaaaaataac gtcgcacggt tgcaggaaga
     8941 tcacgacaac gccgcctgga tggcggagca actgcgtgaa gcaggcgcgg atgtgatgcg
     9001 tcaggacact aatatgctgt ttgttcgcgt cggggaagaa aatgctgccg cgttaggcga
     9061 atacatgaaa gcgagaaacg tgctgattaa cgcctcgccg attgtccgcc tggtgacgca
     9121 tcttgacgtc tcgcgcgaac aactggcgga agtcgccgcc cactggcgcg cattcctggc
     9181 gcgttaagga gagagacgtg ccgcaacgca ttttagttct cggtgccagt ggctacattg
     9241 gtcagcatct ggtgcgcaca ctcagccagc aagggcatca gatcctggcg gcggcacgtc
     9301 atgtcgacag gcttgcaaag ctgcaactgg caaacgtcag ttgccataaa gtcgatctca
     9361 gctggccgga taaccttcct gccctgttgc aggatatcga taccgtctat tttctggtgc
     9421 acagcatggg cgaaggcggc gattttatcg ctcaggagcg ccaggtggct ctcaacgtcc
     9481 gcgatgcgct acgtgaagta ccagttaagc aattaatctt tctcagttcg ttgcaggccc
     9541 cgccacatga gcagtcggat catctgcgag ctcgtcaggc tacggcggac attcttcgtg
     9601 aagcgaatgt accagtgacc gaacttcggg ccggaattat cgttggcgca ggttcagcgg
     9661 cgttcgaagt catgcgcgat atggtctaca acctgccagt gttaacgccg ccacgctggg
     9721 tacgttcacg caccacgccc atcgcgctgg aaaacttgct gcactatctg gtggcgctgt
     9781 tagaccatcc agccaacaaa caccgcatct tcgaagccgc cggaccagag gtgctcagtt
     9841 atcagcaaca gtttgaacat tttatggcgg tgagcggtaa gcgccgctgg ttgatcccca
     9901 tccccctccc cacccgctgg atttcggtgt ggtttctcaa tgtgattact tccgtaccgc
     9961 ccaccaccgc cagggcgttg attcaggggc tgaaacacga tctgctggca gatgataccg
    10021 cgctacgtgc actcatccca caacggctga tcgctttcga tgacgcggta cgtagcacgt
    10081 tgaaagagga agaaaaactg gtcaactcca gcgactgggg ctacgacgct caggcctttg
    10141 cccgctggcg accggagtac ggttatttcg ccaaacaggc ggggtttacc gttaaaacgt
    10201 ccgccagcct tgcggcttta tggctggtgg tgaaccaaat cggcggtaaa gagcgttatt
    10261 tctttggcaa tattttgtgg cagacacggg cgttgatgga ccgcgcgatc ggtcataatc
    10321 tggcgaaagg ccgcccggag cgcgaatatt tacagaccgg cgatgcggtg gatagctgga
    10381 aagtgattgt cgttgaaccg aaaaagcaac ttacgttgtt atttggcatg aaagcgccgg
    10441 ggctgggacg actgtgtttt accctggaag ataaaggtga ctatcgtact atcgatgtcc
    10501 gcgctttctg gcatccgcac ggtatgccgg ggctgtttta ctggttattg atgatccccg
    10561 cgcatctgtt tatttttcgc ggaatggcaa aacaaatcgc cagactggca gaacaaagca
    10621 cagattaatc gacaaaacct gcgttattct ttcatgtttc ccattgcatc atgggggaaa
    10681 tcacggaaga atgcgcacgt cattttctca acaacagtgg attgatatga aggtactggt
    10741 taccggcgcc accagcggct taggtcgaaa cgcggtagag tttttatgcc agaaaggcat
    10801 cagcgtgcgt gcgagcggtc gcaacgaagc aatgggtaaa ttgctggaaa aaatgggcgc
    10861 agagtttgtt ctgacggatc tgaccgagct ggtttcatca caagctaaag tgatgctcgc
    10921 gggcattgat acgctgtggc actgctccag ctttacctca ccctggggga cacaacaggc
    10981 tttcgatctg gctaacgttc gcgccactcg ccgcctgggt gaatgggctg tcgcctgggg
    11041 tgtgcgtaac tttattcata tctcttcccc ctccctgtac ttcgattatc accaccatcg
    11101 cgatattaaa gaagattttc gccctcaccg cttcgccaac gagtttgccc gcagcaaagc
    11161 agccagcgaa gaagtgatca atatgctttc gcaggcgaat ccacaaacgc gctttactat
    11221 tctgcgccca caaagtctgt tcggaccgca cgataaagtc tttattcccc gtctggcgca
    11281 tatgatgcac cactacggca gtattctgtt accgcatggc ggcagtgcgc tggtcgatat
    11341 gacctactat gaaaatgccg tgcacgcaat gtggctggca agccaggaag cctgcgataa
    11401 gctaccttcc gggcgtgtgt acaacatcac caacggcgag catcgcacac tgcgcagcat
    11461 cgtgcagaag ctgatcgacg agttgaatat tgactgtcgt attcgttccg tcccttaccc
    11521 gatgctggat atgatcgccc acagcatgga gcgtttaggc cgcaagtcag caaaagagcc
    11581 gccgctgacc cactacggcg tctccaagtt gaattttgac tttacgctgg atattacgcg
    11641 tgcgcaggaa gagttaggtt atcagccggt catcaccctg gatgaaggca tcgagaaaac
    11701 tgccgcctgg ctacgcgacc acggaaaact gccgcgctaa tcctgcccgt atttctccag
    11761 caatgcttcg gcaatcgcct gagtttctgc atccgcttcg ccgttatata acgtcgggcg
    11821 gaaatgcatc tggaatgcca taatcacgcg ccgctgctcg cgcggtgtca tatcaggttt
    11881 tacgtcataa ccgtagcgcg ccaaaagctc cagcaatgac gcggtatcta ccggtgtgtg
    11941 cggcgcgcgc ccggcaaggt aaaagttcac ccgctgcgca tccggccagg caccaatccc
    12001 ctgctgcgcc agttgctgcc agggaaataa tggccccgga tcgtctttgc gctgcggtgc
    12061 gatatccgca tgtgccacta cgttttccgg cttgatgtga taacgggcaa taatatcttt
    12121 cgccagcgga ataagcgcct gaatctgtgc ctgttcaaac ggggcaaaat atttcactcc
    12181 ggatgatttt tgccagccac ggttttccag ctcaatgcca atagaggtgt cgttaaggcg
    12241 cgttgccccg cgccaggcgc taatccccgc atgccaggcc agttcttgtt ccggcaccag
    12301 ttgccagatg cgcggtttac cgttgtatcg cggtggcacc gcagggatca gataatgcga
    12361 gctgacctgc ttatcagtca gtgtcgccag cgagctatca aaatcatctg cggtgtagtg
    12421 gatcaccagc actttaatgc gcggatacgc cgcctgcgcc tggtgtcggg tatcaagctg
    12481 atatccctct ttctcgacaa tgcctttttc gcctgcacac cctgccagta acagagcggc
    12541 agcgaccagc cagaagactc ttctcatcga cgcgttttca ccgccgtacc gctgacgcta
    12601 accatcagca tactgccgtt ttgcccgacc gtttcgtagt cgatatcaat accaactacg
    12661 gcatcggccc ccagcgcccg cgcctgggag cctaattcct caaaggcgat ctcccgtgct
    12721 ttacgcagtt ctttttcata cgcaccggaa cgtccgccaa cgatatcgcg gataccggca
    12781 aagaaatcac ggaaaatatt ggcacctaaa atcgcttcgc cggtcaccac accgcaatat
    12841 tcaacgatgg tctggccttc cagagttggg gttgtagaaa attgcatgat cgatctcctt
    12901 gtttgttaag ccttcgatct ctaaagcatt atcggactga tatgccatgt tgggaagaag
    12961 gttttgatca gtttaggaaa tcatctcatt gtgtcaaaaa cacccactta agtctttacg
    13021 attatccgtt gcataacccg atattggttt cattataggg taccgcttac ctgtcttatt
    13081 atgttgcacc aacaacatta atgagcggaa agaggcaact tatattgtaa atgactccta
    13141 aagtcttccc catcttgtga ctgaggtcta taccgctgga ttgctttttg gtcagccgcc
    13201 gcaaaacaag aaagtaataa gatctcaatg cttttgtcat taatataagc gaagatcatt
    13261 cttgcaaact cgtatataaa cgcccgatcc tgagcgctta atagtttaat acaatattta
    13321 tttttaagag agaaaataat ttcaaggaaa gcatccgcag caaccttcac gttattcccg
    13381 gcgttcatat tatatatatc ccttgcacag atagagtaca gagatagtaa acagccatac
    13441 aaataatcac ttattaattc ttttgtccat tttacaggca accagggttt tatcttgaca
    13501 ccatgcatac tacattcatg aaaaagacat ttgctgagat taactcttat catatcaaca
    13561 ttgaaaaaat tgcagttaat aaattttaaa gaatgagtat ttacgttatt aattttaaca
    13621 gatctaaacg aaatattttt gaatgcactg tttattacat tgctatttgt tattttaaca
    13681 tcacctaaac tgccgttttc aaaacaacat tttttcaata tgctttcatt cacaacagtg
    13741 tgtgataaat agagattact tatgttttgc tttattaatg ccacgtcatt aaaatgacta
    13801 tatttaatga aacaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
    13861 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
    13921 aagtgaatca tgcattgcgt tccctttgtc atatccacac ttacgctaca aatatcgtgg
    13981 taatgcgaag agtgcatttc aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat
    14041 cgtgttaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
    14101 agcactattg catgctctac atctaacgca gtttcaaatg actcttcttc tgtaaaatca
    14161 ttatttaaaa cctcacctga cgtattatca ctgtcataaa aagtgctcat caatgcgata
    14221 ttgtttgtta cagcgtcttt atcgatattc tcactaatag catatcgcct gctataatca
    14281 taaaaatgaa taaatgcgtc ctttataaag gatatatatt gttctttgtt aaagtcaata
    14341 cgactgtata acttttcaaa aatcacattc gcaatcttac taccaatggt atacctgtat
    14401 aataatgggt gcgattcaat cgtaaaatac ttatctaatt gttcttgtaa tttattctca
    14461 tagtccagtg ggaaaagaaa ctgttgtctt tcaagttttc cattcgatac attacgtatt
    14521 ccaatagaga ttctttcatt acccgctttt atttcaactt cgttatcttc tgatttaccc
    14581 agatattttt taacatcaat ctcgaaagca ttgctaaaaa atgtattaaa ttggtaattc
    14641 gctgatggaa tattttccgc gagcaacaca tcatttttta tgagatcact gtcagcattt
    14701 attgctaata tcagatgcaa gtagatggta tcccatgtag acaactcacc cttacgactg
    14761 gaatttaata tcgcgaaaaa ttccctcagt gaatccagga aattatcact ttgatttagt
    14821 tgtgtgcctg gaaatgatga tgcttgatca gatatattgt ttatcatatt aaaaatcatc
    14881 caagaaaaac gataatccac cgaactcctc gcgctttata attataaaag cacaacacct
    14941 tttgtattca tattaattaa cgaaatactg aagcaattta atttattctt cactcaaatt
    15001 cattagctta aattctttac ccacaattgt agcataaata atattatcga gagtcaaagc
    15061 tgggactatc aaatttcgct cattttcaaa aatacaacgt aattttggca atcatcttaa
    15121 tcgcaggatc tcttatcgca tgtgaaaaat tttgtaatga ttagacaata accgcattca
    15181 tttatcacgc gttataaatg tgaaactggc ttcttgcaga tgctcttaac ctctacagga
    15241 ggatttatac caccatttca gaactatcat atttacctta aatgatcatt tcagccatat
    15301 aacacgctca acctcacaaa tggtttacac tgtctattgt ttgggtaatt taagtaagtc
    15361 agctacgagc aaccttgagt gggtagccag caatggactc aaacccaatc aggtgttcag
    15421 attgtttcag aattgtatta aaaacaatgt attaaataat ctgatagcgt ttggtcgtag
    15481 tgtgacgcag acagcaagct aataaggaaa tgattatgcg ctaccgttca ctatctctac
    15541 ttattccatg tgcactgctg ctgagtgcct gtaccaccgt cacgccagct tataaagata
    15601 acggcccccg caccggttcc tgtgtccagg gagggcctga cagtgtggct caacaattct
    15661 acgactaccg cattcaacat cgcagcaatg acattaccgc cctgcgcccc tatttgagtg
    15721 acaaactggc aacccaactt agcgatgcca gccgcgataa tagtcatcgt gaattgttaa
    15781 gcagcgatcc gttctccagt cgaaccacct tgccagacag cgcccacgtt gccagtgcat
    15841 cgactatccc taatcgtgat gcccgtaaca ttccattgcg ggtggatctc aaacagggtg
    15901 accagggctg gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg
    15961 atgtgcgcta cttaggcggt agcgttcatg ccacggcggg tacgctccgt cagtccatcg
    16021 agaatcgcta acgccttgat caaccaataa accacgtaaa ttatccggca tttcgtgagg
    16081 aatgccgaca tttatgctca ccgaccaccg cccccgttat tttgtgctat gtttattgaa
    16141 taatgcgctt tgcttttaac ttttaaagca taaatattgc ataattattc tgtcaaaggt
    16201 actatcttcg acctcaatag ctatcagact gccagtatac gagtgtcaat gagtattcaa
    16261 ttaaacggca ttaattgctt ctacggcgcg catcaggcgc tgttcgatat cacgctggat
    16321 tgcccacagg gcgaaacgct ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg
    16381 ctgcgtgtac tcaatctgct tgagatgccg cgctccggta cgctcaacat tgcaggcaac
    16441 catttcgatt tcaccaaaac accctctgac aaagcgattc gcgatttgcg tcgtaacgtt
    16501 ggcatggtgt ttcagcaata caacctgtgg ccgcatctga ccgtgcagca aaacctgatt
    16561 gaagcgccct gccgtgtact ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa
    16621 ctgctggaac gtctgcgtct caaaccttat agcgatcgtt acccgctgca tctttctggt
    16681 ggtcagcagc agcgtgttgc tattgcccgt gcgttgatga tggaaccgca ggtactgctg
    16741 ttcgatgaac cgaccgccgc actggacccg gaaattacgg cacaaatcgt cagcatcatt
    16801 cgtgagctgg cagaaacgaa tattacccag gtgatcgtca cccacgaagt tgaagtggcg
    16861 cgtaaaaccg ccagccgagt ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac
    16921 gcgagctgct ttaccgagcc gcaaaccgaa gcatttaaaa actatctctc tcactaagat
    16981 tcgggataac aacaatgaaa aaagttctga ttgccgcgtt aattgcaggt tttagtcttt
    17041 ccgccacagc tgccgaaacc attcgttttg ctaccgaagc ctcctatcct ccgtttgaat
    17101 cgattgatgc aaacaaccag atcgttggtt ttgacgtcga cctggcacag gcactgtgta
    17161 aagagattga tgcaacctgt actttctcta accaggcgtt tgacagcctg atcccaagcc
    17221 tgaaattccg tcgcgtagaa gcagtgatgg cgggcatgga tatcactccg gagcgtgaaa
    17281 agcaggtact gtttactacc ccgtactatg acaactctgc cctgtttgtg ggtcagcaag
    17341 gcaaatacac cagtgttgat cagctgaaag gcaaaaaagt cggcgttcag aacgggacga
    17401 cacaccagaa gttcattatg gataagcacc cggaaatcac caccgttccg tatgacagct
    17461 accagaacgc aaaactggat ctgcaaaacg ggcgtatcga cagcgtattt ggtgacaccg
    17521 cagtggtcac tgagtggctg aaagataacc caaaactggc ggcagtgggc gataaagtga
    17581 ccgataaaga ttatttcggt actggcctcg gcatcgcggt acgtcagggc aacactgaac
    17641 tgcagcagaa actcaacact gcgctggaaa aagtgaagaa agatggcact tacgaaacca
    17701 tctacaacaa atggttccag aagtaattcc tgatgaatga attttttcct ttagcaagcg
    17761 ccgccgggat gaccgtcggc cttgccgttt gtgcattgat tgtcgggctg gcgctggcga
    17821 tgttctttgc ggtatgggag tcagcgaaat ggcgtcctgt cgcgtgggca ggttcagcgc
    17881 tggtaaccat tctgcgtggc ctgccagaaa ttctggtagt gctgtttatc tattttggct
    17941 cctcgcagtt actgctgacg ctttcggatg gcttcactat caatcttggg ttcgtgcaga
    18001 tcccggtgca gatggacatt gagaacttcg acgtgagtcc gttcctttgt ggtgtcatcg
    18061 ctctgtcact gctgtatgcc gcctatgcct cgcaaacgct gcgcggcgcg ttgaaagctg
    18121 taccggtggg ccagtgggaa tctggtcagg cgctggggct gtcgaaatcg gctatctttt
    18181 tccgtctggt gatgccgcag atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc
    18241 tggtgctgct gaaagatacc gcgctggtca gtttgattag tgtgaatgat ttaatgttac
    18301 aaaccaaaag cattgctact cgtacccagg aaccatttac ctggtacatt gtggcggcgg
    18361 cgatttacct ggtgatcacc ctgctcagtc agtacattct caaacgcatt gacctgcgcg
    18421 cgacacgttt tgagcggagg cccagctaat gtttgagtat ttacccgagc tgatgaaagg
    18481 gctgcacacc agcctgacgc taaccgttgc ctcgctgatt gtggcactga ttctggcatt
    18541 gatttttacc atcatcctga cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta
    18601 tatcacgctg tttaccggta cgccgctgct ggtgcagatc ttcctgattt attacgggcc
    18661 gggccagttt ccgacgttgc aggagtatcc ggcactgtgg catttgttgt cagaaccgtg
    18721 gttatgtgcg ctgattgcgt tatcgctgaa cagtgcggcg tataccacgc agctgtttta
    18781 cggtgcaatt cgtgcgatcc cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag
    18841 caaaaaagat acgctggcga tcctgctgcc gtatgccttt aaacgctcgc tctcttctta
    18901 ttccaacgaa gtggtgctgg tgttcaaaag tacctctctg gcttacacca ttacgctgat
    18961 ggaagtgatg ggatacagcc agttgttgta cggacgcacc tacgatgtaa tggtgttcgg
    19021 tgcggcaggg attatttacc tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat
    19081 cgagcgcaaa gcactggcat tcgaacggcg gaattaatgt agctgcgtac attcgccggg
    19141 gggatttgta ggtctgataa gacgcgccag cgtcgcatca gacatcagca cggtgcctgt
    19201 gccggatgcg gcgtggacgc cttatccggc ctacatagtg cataaattct tataatgaag
    19261 acggacaact aactaagtta tccgtctttt tttatttcat ttaaattatt taatcatgtt
    19321 tattgcatat aaattcactt ggtggcattg ttatcccatg ccgcagacac ggccaaaaat
    19381 cataagattg acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc
    19441 ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta
    19501 tccacccttt gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc
    19561 aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt actaatcacg cgttcgacag
    19621 cctgatcccg tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac
    19681 cccggagcgt agcaagcagg tttcgtttac cacaccctac tatgaaaact cagccgtcgt
    19741 gattgccaaa aaagatacct acaaaacgtt tgccgacctg aaaggcaaac gcattgggat
    19801 ggaaaacggt acgacgcacc agaaatatat tcaggatcag cacccggaag tgaaaactgt
    19861 ctcctatgac agttatcaga atgcctttat cgatctgaaa aatggtcgta ttgatggggt
    19921 atttggtgac accgcggtgg taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc
    19981 gactgaaaaa gtgaccgatc cgcaatactt cggcaccggc ctgggtatcg ctgtacgtcc
    20041 ggataacaaa gctctgctgg aaaaactgaa taacgcgctg gcagcaatta aagctgacgg
    20101 tacatatcag aaaatcaatg accagtggtt tccgcagtaa ttgagactga tgacaaaagt
    20161 aagattgcct gatgcgctgc gcttatccgg cccacatcaa ctctgcaatg tattgattta
    20221 gcatgatttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcacc
    20281 tgatcagcaa tctgaagccg cacctgtggc ttttttattg cttcaccagc agcgtcaaca
    20341 cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcggtaac
    20401 ctggcagttc gcggatatct ttcgccatgg tttgggcgtt acagctggag tagataataa
    20461 aacgcggtgc catcgttgaa agataatcac acagcggttt accaatgccg cggcgcggcg
    20521 ggttaaccag caccagctcc ggcacctccc cctgagcagt ggcaaactga gtggagtcca
    20581 gcgcctgaaa ttgtaaacga gttaagccca gttcagcggc tgactgcttt gcacaggcaa
    20641 tggcctctgg cgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac
    20701 caaagccccc cacaccgcag aacagatccc acatatgttt aaccggcagc tgtcgtaccc
    20761 agtcgcgcgc ggtggcgtac aactggctgg cgaccgccgg attagtctgg aagaaacttt
    20821 gcggacggat ccacagcggt acgtcattaa aacgctccgc cagtgcctgt tgttcggtca
    20881 ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa
    20941 taactttcag ctgcggtagt tgttcgtgta accacggcag cgccttacgc agttgcgcca
    21001 gcttggtatc agaacgcagt acaaagcgca gcatcatgcc gccatcactc tggctttcag
    21061 tcagcagaat gtatttcagt tcgccacgct tacgcgccac gttgtagggc gttaaccccg
    21121 ctcgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaatgaggct ggataaagcg
    21181 ggcagtcaca aaggtcttcc ggcgtgccat cgcgatgcag cataccgagc agtgtttttt
    21241 caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc gggcctgaca
    21301 ccggcgcgca ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggttt
    21361 tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg
    21421 cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca
    21481 cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg
    21541 agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg
    21601 ggaatgctga acagctccgg ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg
    21661 gcggtgattt gtgtcagcag atatagccag cgcgcccagc gacgcccttt caccagtgaa
    21721 aacgcacacc agatctcaat gaaaaccagc accagactgc ttaaaaagac cagcgttaaa
    21781 ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg
    21841 aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc
    21901 agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt
    21961 tcgtcttcca tgaaaacaat gccgcgacat gcgcagcatt atgtagccag gttggcaaat
    22021 tttagtgtct tcagccacgt cttgcacgtt ggatgtcgcg tatccgctgt ttttctgcgc
    22081 gcgccatcag ataccaggcg ataaatccga caattccgac cgcgccgagg atcaatgttg
    22141 ccagggcgtt gatttccgga ttcaccccca tccgcacgct ggaaaagacc agcatcggta
    22201 acgtggtggc tcccggcccg gaaacaaagc tggcgatcac cagatcatca agcgacaaag
    22261 taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga
    22321 aaaacacttt cagcggcgtc gcaccgagat ccatcgcggc ttcttctatc gagcgatcca
    22381 gttcccgcag acgcgacgaa ataacgaccg ccacataagc cgtacagaaa gtgacatgcg
    22441 ccagccagat ggtaagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga
    22501 cgaataataa caacagcgac aagcccgtga tgacatctgg cattaccagc ggcgcggtga
    22561 tcataaaggc aaagccattt gatccgcgaa acctgccaaa gcgcaccagc accaccgccg
    22621 caatagtccc gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc aggcttgaac
    22681 caaccgcact catcatcgcg tcatcgcgca ataactcacc ataccagcgc gttgaccagc
    22741 cggcccacac cgtcaccagc ttcgagctgt taaacgaata gataaccagc atcagcattg
    22801 gcgcgtagag aaaggtaaag cccagcagca aaatcacaat ccgccagggc gaacgaacta
    22861 ccggtaaatt attcatccgt gttctcccac gcttttttgc tggtgtttgt ggaaccacat
    22921 tatcggcaca attagcagca gcaacatgat gatcgctacc gccgaggcca ccggccagtc
    22981 gcggttattg aaaaactctt gccatagcac gcgcccgatc atgatgctgt ccgggccacc
    23041 gagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc
    23101 aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc
    23161 accgagatcc agcgctgctt ccaccagcga ataatcaata cgaatcaacg cggtataaat
    23221 cggcagcacc ataaacggca cgtaggcgta aacaatgcca atataaactg ccagattggt
    23281 atgaagaatg gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac
    23341 accgttgttt tttaatattc ccatccaggc ataaacgcgg atcagaaacg aggtccacga
    23401 cggcaggatc accagtagta ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc
    23461 ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag
    23521 cgactggaga taagcatcga aatagagcgg atcgtcggtc agttgcagaa aattaccgag
    23581 attaagagtg atggacagtt gcccgtcagc ccactccatc agttcggtat aaggtggaat
    23641 agcgcgcgcc atctccgcca ggcttatttt aaagacaatc agaaatggca gcagaaacag
    23701 caagatcaac cagatatatg gcaacgcaat gaccagtttg cgcccatgct tcatttgcag
    23761 ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt
    23821 attcatcttt gctccttaaa ccgtcagcac cacactgctg tccacttccc agcacaaacg
    23881 cacttcgtcg ccctaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc
    23941 gctaatcatc tgcccacttt tcagacgaac gtgatacacc gaaagatcgc cgagataggc
    24001 aatgtgtatc acctccccca ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag
    24061 catgattttt tccgggcgca gcgccacatg taccggcacg ttatcgacca ccgaggcatc
    24121 cgcgtcgact ttcagtggat gcaccagccc cggcgaatca agcaccaggc catcttcctg
    24181 acgctctttg agtacgcctt caaagacgtt taccgagcca ataaattcgg cgctatagcg
    24241 ggtagtcgga tgctcgtaga tctcttccgg ttcgccaatc tggacaaatt tcccacgatt
    24301 cataatggcg atgcgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac
    24361 acaagtcaca ccaacgcgct ccagaatatc caccacttca agctgcatcc ggtcacgcag
    24421 ctttttatcc agcgcgccca tcggctcgtc gagcagtaat agtttcgggc gcttggcaag
    24481 gcttcgggcc agggccacac gctgtcgctg accaccagaa agctgatgcg gtttacgttt
    24541 ggcgaactcc tgcatgtgca ccaggccgag catctcattg acccgactgg aaatttccgc
    24601 tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg
    24661 aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg gaacctgtga
    24721 caaatcgacg ccatcaagca ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat
    24781 acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt
    24841 gtagatggtc aggctgacat catccaccgc atgttgacca tcgtaggatt tggtgaggtt
    24901 gcggatttct aatagcggcg tcagcacctt acgggttttc gcctgcgggc gagggatagc
    24961 gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt
    25021 gccggcgggt gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt ccgctcttca
    25081 ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact ttcagagtga
    25141 acagcttcgc acgaacatcc gcaggcggat aaatacccgg gttctcacgg acttccgcac
    25201 tcaccagcgg cgtggctgct ttattggcgt tgacatagaa cacatggtcg gaaatatgcg
    25261 ctactacatc cgggcgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg
    25321 catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga
    25381 acgagacatt cactccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac
    25441 ctgcccagcc gatagcgacg caaatatcgc cgtttgccag gtcgtcaatg tattgagatg
    25501 aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggttgcc ggtccggtgt
    25561 aatcatccgc tttagtgctg ttgggatctt tgccgagata attcaacacg gtagcaaaaa
    25621 cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag
    25681 gtttgaggat caagtcccag ctatcgacgg gcgcgttttc gcccagcaca gctttaactt
    25741 tatcaacgtt atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt
    25801 cgggatcgtg tttggcgacc agcttcagca gctccgggtc gagattcttc cactccggca
    25861 atttgctttt gtccagcggc tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg
    25921 cagatggaac caccaagtca aagccggtgc tacccgccat taatttgcct tccagtactt
    25981 cgttagagtc gaaaacatcg tagacgactt taataccggt ttctttttca aaattggcca
    26041 ccgtgtcagg ggcgatataa tcagaccagt tataaatgtg gagtgttttt tgttcagccg
    26101 cgagcgtgcc gacagagacg gccatcagag cacccgcaac cagacccgat agccattttt
    26161 tatttaaggc ggtcacatct ctttccttct gaaagttcgt taacaaacga acaaaagttg
    26221 tcacactgaa agatatgcaa taaacgtgca tattttttta tcggcgcggt gtaagaaaag
    26281 agaactctct cccggccagc actgctcgct atatagaagc atcgcaagaa taactttagc
    26341 cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt tatgcgtatt
    26401 tgctctatgt gataaagcca aatcgacaca agagaagtga aataatcttt aagaaaaggt
    26461 taaaaacagc agataatttt gcagcgcgca gtcactgcgg cagcgactgc gtgctcgaaa
    26521 ggaattatca gtggagaaaa tagttttcgg taacgttgtt ttgcccttca tcgtcagtcg
    26581 gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc tcttcgctct
    26641 gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat aaagactggc
    26701 aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc acggtcagcg
    26761 aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa ggtcggactt
    26821 ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca tcaaagacac
    26881 cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttattacat tcaaaaaaac
    26941 tcatccccag gacatcgagc cattgacgca gcgtatccag accaggaacg accagcgatg
    27001 tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc aaaaagcgca
    27061 ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg caatattctg
    27121 tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatatcgata ccggtggttt
    27181 tttctattcc ttccagcccc ggcgacgcat tcacttccat caccaacggc ccgcgattag
    27241 cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct tttatcgcga
    27301 tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga tgcaaattgg
    27361 aacgaaaata gccctctttc gcccgccgtt caatcgcagc gaccacttca tcaccaacaa
    27421 ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc agaatatgcg
    27481 cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctgacgagtc tccgccagca
    27541 cgacgccaat tccctgcgtg ccttcaacca acttgaccac cagcggcgca ccaccgacca
    27601 tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca ggcaggtcga
    27661 tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg gcaatggcga
    27721 ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc gccgtcccat
    27781 aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt aacttgcggc
    27841 ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa agcggatcaa
    27901 gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta cacgaataga
    27961 gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc agacctggtc
    28021 aaaagcccgc gtatcataca tcgacgtggc ggttttagcg cgtcgcccaa ccctgtttgt
    28081 gcaaataatc cagaataaat gggcggcttt ctttaatgat tgttcggcgg atatgatcgc
    28141 tccaggtatc ccagcgatta ttgctgccac gggtgaggta atattccgcc agttgctcgt
    28201 catactgcgc cagtgcgtct ttatccagcg gttgatagct gttttcatgc accaaaatgg
    28261 aggccggtaa acgcggcttg agatccggat tatccgcagg ccagccaagg cacagcccaa
    28321 acagcggcag aacatgctgc ggtaatttca gcagtttcgt caccgcttca atattattgc
    28381 gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcggtt aatgcattct
    28441 gcgccatcat tgccgtatca acgacaccga gcaacagttg ttccgccagg ccgagctgag
    28501 catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag aactccgccg
    28561 cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcttcacgt aatgctttgt
    28621 cggtaatgcg aataatgcta ctacactgca aaaaactgga actggacgtc gcacgggcgc
    28681 tgttaataat cgcctcacac tgcgcttcgg aaatgggttc atcagtgaaa tggcgaatgg
    28741 agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct ttctgaacgt
    28801 gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttaccac ctgaagaata
    28861 accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag aatctgcact
    28921 tctggccgag aaaatggctc aggcagcgac agggagtcgc ttaccgacag cagcgccacc
    28981 gccagtagca tcattccgat aaattccagt atcaacacgc ctttaggcaa tttaccgatc
    29041 gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc actgtattgt
    29101 gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt tcagcgaata
    29161 cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg caaaccgtta
    29221 tttttggtcg ttcgggttgc ccttactgtg tgcgtgcaaa agatctggct gagaaattga
    29281 gcaatgaacg cgatgatttt cagtatcagt atgtagatat tcgtgcggaa gggatcacta
    29341 aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag atttttgtcg
    29401 atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagaa aatctggacg
    29461 cctgatcgtc tgacaagccc tcgcgttgag ggctttactg attttttctg tgctgtggtt
    29521 taaacaaact actgataaat aagaaacaca gtgcccccag cgcacaccag aacaccgcgc
    29581 ttagtaacca tgccagctct tgccagaatg agcgcgtcgg tgaaaaaaac agccgcataa
    29641 tgagcattga acagggtgcc gccagcattg cgccaaatag aggtttcagg acttctctac
    29701 gctgtgaaaa aaaactggca actgctccag gaagaatgaa aaacagcaag ccgatttcag
    29761 gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaagg cacaccacaa
    29821 tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc actcgttcct
    29881 cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta agataaagcc
    29941 gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga tacctaatgt
    30001 gattacacta gtaaaatata ttgttacttt actatcgttt aggtgcgctg aatgaatctg
    30061 caccctgaat tctggtaaaa cacattatcg taaattacca tttctttcaa cagcttacta
    30121 gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa tgggaattac
    30181 attctgttat tatttgtggt cctcgcgctt gggctatgtc tcggaaagtt acgacttggt
    30241 tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt aggccaacaa
    30301 catttcagca ttaacaccga tgcgcttaat cttggcttta tgctgtttat tttctgcgtc
    30361 ggggtcgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa aaattaccta
    30421 atgttagcac tggtgatggt tggcagtgcg ctggtgatcg ccttagggtt aggtaagctg
    30481 tttggctggg atattggcct gacggccggt atgttagcag gctctatgac gtcgacaccg
    30541 gttctggtcg gtgctggcga tacactgcat cattccggca tggaaagcag gcagctctca
    30601 ctggcactgg ataatctgag cctcgggtat gctttaacct atttaatcgg tctggtgagt
    30661 ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca gaccagcgcc
    30721 cagcaaatcg cccgcgaacg tggcctggac actgatgcca accgtaaggt ttatttaccg
    30781 gtgatccgcg cctatcgcgt cggcccggag ctggtggcct ggaccgacgg caaaaatctg
    30841 cgtgaactgg gtatttatcg acaaaccggc tgctacattg aacgtattcg acgtaacggg
    30901 attctggcaa atccagacgg tgatgccgtg ctacaaatgg gcgatgaaat agcgttggta
    30961 ggctatcccg acgcccatgc ccgactcgat cccagcttcc gtaacggtaa agaagttttc
    31021 gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa aaaccataac
    31081 gctgtaggta aacgtctcgc acaactgaag ttgaccgatc acggttgctt ccttaaccgc
    31141 gtcattcgta gccagattga gatgccgata gatgacaacg tcgtgcttaa caaaggtgac
    31201 gttttacaag tcagcggtga tgcccgtcgc gtaaaaacca tcgccgatcg catcggcttt
    31261 atctcgattc acagccaggt cactgacctg ctggcatttt gtgccttctt tgttattggg
    31321 ctgatgatcg ggatgatcac cttccagttc agcacattca gtttcggcat ggggaacgct
    31381 gccgggttgt tattcgccgg aattatgctg ggctttatgc gtgctaacca cccgaccttc
    31441 ggttacattc cgcaaggtgc attaagcatg gtgaaagagt tcggcttgat ggtgtttatg
    31501 gcaggcgttg gtctgagcgc cggtagcggt attaataacg gcctgggcgc gattggcggt
    31561 cagatgttga ttgccggatt aattgtcagt cttgtgcccg tggttatctg tttcttgttc
    31621 ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg gcgcaatgat gggcgcacgc
    31681 acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaacat ccctgcgctg
    31741 ggctatgcgg gcacctacgc aatcaccaac gtcctgctga cgctggcagg gacaatcatc
    31801 gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga aatttttttg
    31861 cacaaccgca gaacttttcc gcagggcatc agtcttaatt agtgccactg cttttctttg
    31921 atgtccccat tttgtggagc ccatcaaccc cgccatttcg gttcaaggtt gatgggtttt
    31981 ttgttatcta aaatttatct actaccctgc aaccctctca accatcctca aaatctcctc
    32041 gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc atcccctcaa taaacgcatc
    32101 aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc gtttgctgac tgcgctgcat
    32161 ccagttttgc attaccgttt ttaatagcgg ttttcggcta gccagcgcgt agagctggta
    32221 catcagctcc atattatccg gcgttgcaac ctgtgagctg tagatcatat cggtgatagc
    32281 ctggcatgcg ccttgagcat cactaacatc gctaaaaaat gcctgatatt gccgggacat
    32341 aatctcagta aaactgctga acgcttccag caacaactca tcaattcctg aaaaatagta
    32401 ggtcatcgat cccaacggta ccccggcaag ggtagcaatt ttgcggtgcg taacagcatg
    32461 tattccgtaa agtttcaccg cctccagcgt ggcctggata attttttctc gccgttgcgg
    32521 atcgttagcg cgacgcatgt catttcctct gtctgcaatt gtgtacaaat gtacacaacc
    32581 ttgctaatgt tgtcttccct cttcttattt tgacgtgatc tatgaccgta aattcttcac
    32641 gtaatgcatt gaaacgccga accagggcgc tgtttatgtt cttctttttg ccaggcctgt
    32701 taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga tattctctct gtctcgatcg
    32761 ctgaaatggg cggtgttctc tttggtctgt cgatcggttc gatgagcggt attctctgct
    32821 cggcgtggtt agtgaaacgc tttgggacac gtaatgtcat cctggtcacg atgtcctgcg
    32881 cattgatcgg gatgatgata ttaagtctgg cactctggct gacatcgccc ctgctctttg
    32941 ccgttggtct cggcgtcttt ggggcaagtt ttggttctgc ggaagtggcg ataaacgttg
    33001 aaggtgccgc cgttgagcga gaaatgaata aaacggtttt gccgatgatg cacggttttt
    33061 atagcctggg cacgctggca ggcgctggtg tcgggatggc actgacggcc tttggcgttc
    33121 cggcaacggt gcacatttta ttggcggcgc tggtaggtat cgcgcctatt tatatcgcca
    33181 ttcaggcaat ccctgacggt acgggcaaaa atgctgccga tggcacccag catggcgaaa
    33241 aaggcattcc tttttatcgc gatatccagt tgctgttgat tggtgttgta gtgctggcga
    33301 tggcctttgc cgaaggttct gccaacgact ggttaccctt attaatggtt gatggtcacg
    33361 gttttagccc cacttccggc tcactgattt atgccggttt taccctgggg atgaccgttg
    33421 gacgcttcac cggcggttgg ttcatcgacc gttacagtcg cgttgccgtg gttcgggcca
    33481 gtgcactaat gggggcgttg ggtattgggc tgattatttt tgtcgatagc gcctgggtcg
    33541 caggagtgtc tgttgtactt tggggactgg gtgcctcgct gggcttcccg ctgaccattt
    33601 ctgccgccag cgataccggc cccgatgcac caacccgcgt cagtgtggta gcaacgaccg
    33661 gttatctggc tttcctcgtc gggccgccgc tgctgggcta tctcggcgaa cattatggat
    33721 tacgtagtgc aatgctggtt gtactggcgc tggttattct cgcggctatt gtcgcgaaag
    33781 ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga gaatagttga tgagtattaa
    33841 attaattgcg gtagacatgg atggtacttt cttaagcgat caaaaaacct ataaccgtga
    33901 gcggtttatg gctcagtatc agcaaatgaa agcacaaggc attcgctttg tggtcgccag
    33961 cgggaatcaa tattatcagt tgatctcttt cttccctgaa attgcgaatg aaattgcctt
    34021 tgtggctgaa aacggcggct gggtagtgag tgaaggcaaa gatgttttta atggcgagct
    34081 gtcgaaggat gcgtttgcta ctgtcgtgga acatttgctg acgcgcccgg aagtggaaat
    34141 tattgcctgc ggaaaaaata gcgcctatac actcaaaaaa tatgacgatg ccatgaaaac
    34201 ggtggcggaa atgtattatc accgtctgga atacgtcgat aactttgaca acttagaaga
    34261 tattttcttt aagtttggcc tgaatctttc cgatgaactg attccacaag tacaaaaagc
    34321 attacatgag gccatcggcg atattatggt gccagtccac accggcaacg gcagcatcga
    34381 tctgattatc cccggcgtac ataaagccaa tggccttcgc caactgcaga aattatgggg
    34441 aatagacgac agcgaagtgg tggtctttgg cgatggcggt aacgatattg agatgctgcg
    34501 tcaggcaggc tttagttttg caatgaaaaa tgccggcagc gcggtcgtcg cagcggcaaa
    34561 ataccgggca ggctccaata accgtgaagg cgtactggat gtgatcgata aagttcttaa
    34621 acacgaagcg ccatttgacc aataaaaacg gcagcacgcc cggcaacaca atgcctgaat
    34681 gaaggctgca taattcgcgg aaattaaccc gctgcattat ttcaggcgaa atgattatga
    34741 aaaattgtct actgttgggc gcgcttttaa tgggctttac tggtgtggcg atggcgcaaa
    34801 gtgtcaccgt cgatgtgccg agtggataca aagtggttgt ggttcctgat tcagtcagcg
    34861 ttccacaagc gtgtcagcgt cgcgactgtg ccgcaaaccg tctatgtggc tcctgtgcca
    34921 gctccggctt accgcccgca tccttatgtt cgccatcttg ccagcgtagg tgaagggatg
    34981 gttatcgaac accagattga cgaccatcac cattaattcg agaatgcctg atcgcacaaa
    35041 tcaatcaggc atttttttac ccttcgtgag aatttcccat ctgtttatct tttaaaaaga
    35101 taaccatcag cgacagccac aaaattccgt tgacaaggtt gaagagatta aacagtccgt
    35161 tgcccccgtt cagccaggca tgtttgctga tttcaatacc gacggtaaag atcagcattt
    35221 gcagcattcc catcgccgca gaaaccgtac ctttactcat atcgctggca aacagggtta
    35281 atcgcaccag tcccgcattc gccagaccaa taccgaaagc ataaatactt aacccggcgg
    35341 tcatccataa atacgcgtgc gatgagataa ccgttgccgc agcagcgacc aacagaccaa
    35401 tcataatcgg ccagccgccc ataataatca gcgaacgtac ggtgcggcgc gaggtcagac
    35461 gcgctaacag caagttaccc gcaattaacg ccccgaaaat aggcacttgc agcaagccat
    35521 attcatagct gctcaactgc tccccggtaa tgatgataat cggcgactgg gcg
//
LOCUS       DADLTI010000021        35306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLTI010000021 DADLTI010000000
VERSION     DADLTI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.021"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1165)
                     /gene="setB"
                     /locus_tag="JN755_000962"
     CDS             complement(<1..1165)
                     /gene="setB"
                     /locus_tag="JN755_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7379844.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVM"
     gene            1533..2663
                     /locus_tag="JN755_000963"
     CDS             1533..2663
                     /locus_tag="JN755_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7379845.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2663..3601
                     /gene="fruK"
                     /locus_tag="JN755_000964"
     CDS             2663..3601
                     /gene="fruK"
                     /locus_tag="JN755_000964"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7379846.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3618..5309
                     /gene="fruA"
                     /locus_tag="JN755_000965"
     CDS             3618..5309
                     /gene="fruA"
                     /locus_tag="JN755_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7379847.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5733..6674
                     /locus_tag="JN755_000966"
     CDS             5733..6674
                     /locus_tag="JN755_000966"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7379848.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6662..7600
                     /locus_tag="JN755_000967"
     CDS             6662..7600
                     /locus_tag="JN755_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7379849.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7694..8945
                     /locus_tag="JN755_000968"
                     /pseudo
     CDS             7694..8945
                     /locus_tag="JN755_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(9016..9714)
                     /gene="yeiL"
                     /locus_tag="JN755_000969"
     CDS             complement(9016..9714)
                     /gene="yeiL"
                     /locus_tag="JN755_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7379850.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9845..10786
                     /gene="rihB"
                     /locus_tag="JN755_000970"
     CDS             9845..10786
                     /gene="rihB"
                     /locus_tag="JN755_000970"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7379851.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10886..12136
                     /locus_tag="JN755_000971"
     CDS             10886..12136
                     /locus_tag="JN755_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7379852.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12192..13280)
                     /locus_tag="JN755_000972"
     CDS             complement(12192..13280)
                     /locus_tag="JN755_000972"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7379853.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13283..14140)
                     /gene="nfo"
                     /locus_tag="JN755_000973"
     CDS             complement(13283..14140)
                     /gene="nfo"
                     /locus_tag="JN755_000973"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7379854.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14214..15263)
                     /locus_tag="JN755_000974"
     CDS             complement(14214..15263)
                     /locus_tag="JN755_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7379855.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15362..16243
                     /locus_tag="JN755_000975"
     CDS             15362..16243
                     /locus_tag="JN755_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7379856.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16449..17918
                     /gene="lysP"
                     /locus_tag="JN755_000976"
     CDS             16449..17918
                     /gene="lysP"
                     /locus_tag="JN755_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7379857.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18210..20201
                     /gene="cirA"
                     /locus_tag="JN755_000977"
                     /pseudo
     CDS             18210..20201
                     /gene="cirA"
                     /locus_tag="JN755_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20233..21069)
                     /locus_tag="JN755_000978"
     CDS             complement(20233..21069)
                     /locus_tag="JN755_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY7379858.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21327..21995
                     /gene="folE"
                     /locus_tag="JN755_000979"
     CDS             21327..21995
                     /gene="folE"
                     /locus_tag="JN755_000979"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7379859.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22012..23169
                     /locus_tag="JN755_000980"
     CDS             22012..23169
                     /locus_tag="JN755_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7379860.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23311..24279
                     /gene="galS"
                     /locus_tag="JN755_000981"
     CDS             23311..24279
                     /gene="galS"
                     /locus_tag="JN755_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7379861.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24630..25628
                     /gene="mglB"
                     /locus_tag="JN755_000982"
     CDS             24630..25628
                     /gene="mglB"
                     /locus_tag="JN755_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY7379862.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSRIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25689..27209
                     /gene="mglA"
                     /locus_tag="JN755_000983"
     CDS             25689..27209
                     /gene="mglA"
                     /locus_tag="JN755_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7379863.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27225..28235
                     /gene="mglC"
                     /locus_tag="JN755_000984"
     CDS             27225..28235
                     /gene="mglC"
                     /locus_tag="JN755_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7379864.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28306..29541)
                     /gene="preA"
                     /locus_tag="JN755_000985"
     CDS             complement(28306..29541)
                     /gene="preA"
                     /locus_tag="JN755_000985"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7379865.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29535..30775)
                     /locus_tag="JN755_000986"
                     /pseudo
     CDS             complement(29535..30775)
                     /locus_tag="JN755_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30969..31208)
                     /locus_tag="JN755_000987"
     CDS             complement(30969..31208)
                     /locus_tag="JN755_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7379866.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31211..31930)
                     /gene="sanA"
                     /locus_tag="JN755_000988"
     CDS             complement(31211..31930)
                     /gene="sanA"
                     /locus_tag="JN755_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7379867.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32080..32964)
                     /gene="cdd"
                     /locus_tag="JN755_000989"
     CDS             complement(32080..32964)
                     /gene="cdd"
                     /locus_tag="JN755_000989"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7379868.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33094..33789)
                     /locus_tag="JN755_000990"
     CDS             complement(33094..33789)
                     /locus_tag="JN755_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7379869.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33786..34184)
                     /locus_tag="JN755_000991"
     CDS             complement(33786..34184)
                     /locus_tag="JN755_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7379870.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34423..>35306
                     /gene="dusC"
                     /locus_tag="JN755_000992"
     CDS             34423..>35306
                     /gene="dusC"
                     /locus_tag="JN755_000992"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY7379871.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICSELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8671 a         8629 c         9321 g         8685 t
ORIGIN      
        1 tcattaccca aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat
       61 aattccagat ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg
      121 acgtgttggt atagagcgtg gtggctgaac ctgcctgacc gggcatcaaa tcctgaaaat
      181 agagcatccc gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca
      241 acagaatggc aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg
      301 cagcaacgcg cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca
      361 tcgtcgggat ttccagcccg gcggcggtcc ccatcatcac accggccagt ttctcgggaa
      421 gatgcagttc gttgataata aatagcggca tgttgatgat gtacaggctg ttcgagcccc
      481 acatcaatgt acaaatgaca aacagcagta gcgtatcgcg acggttacgg cgcggcgctt
      541 caaccgtgcc ggtcgccagc ggaagctctt tttgcatcga cggtaaaaac agccacacca
      601 tcacgccgca aacaataaac gctaccgctg cgctcagata cattaccgta aagctgaaac
      661 ccatcgctaa cgcataagcc agcggtgggc caatgaccca tgccagtgaa acctgagcgc
      721 gtaaaaaaga gctgaacatc accgcctcac gtccggtttt gtcggcatgt tcacgggcaa
      781 gggcaaacat ttgcgggtta gcggtcgagc caaagctgct aagaaagacg ccaacgaata
      841 gcaaaacaaa gtagttgcga ttccaggcaa aaagggtgca ggccagcacg cctaacaggc
      901 agcaaaagac aatcagcgat ttgcgatcgc cgcgcttatc agagcgccca gcgagaaact
      961 gacttaccag aatcccaatg acagcgctgc cggtaaagaa gaatcccacc atcgccggac
     1021 gggcatgtat ttcatcggta aggaaaatac tgagtgtcgg ggtttgcaga gcacccgcaa
     1081 taccggtgag aaaggcaacg attaaaaacg ccgtcgaggt caggtcaaac gatttcgcgc
     1141 tggagactgc gggggagtta tgcatgctgg tcgatcactg gaagaggaag acgcggagtt
     1201 tacgctgctt gtcagaaaat aaacagacag cgaaatcaaa agcgccacga ataatcaaaa
     1261 tgccatttca ctgttgctga ttggctgctg acgctgctga aatgaaattg ctgataacgg
     1321 attttcccat cagcaattag gaaaaatgac aaaaaattgt gcagcacatc aaacttttgc
     1381 tcataacttt acggctttcc ttgcgtgctg aaacagaagt attatgcttt cttgaaacgt
     1441 ttcagcgcga tcttgtcttt aaccctaagc caggttggcg ctttttttct catagaggct
     1501 gaatcgtttc aattcagcaa gagaggagaa ctatgttcca gttatccgta caggacatcc
     1561 atccgggcga aaaggccgga gacaaagaag aggcgattcg ccaggtcgct gcggcgctgg
     1621 tgcaggccgg taatgtagca gaaggctacg tcaatggcat gctggcgcgc gaacagcaaa
     1681 cctcaacgtt cctcggcaat tgtattgcta ttccacacgg cactaccgac acccgcgatc
     1741 aggtgctgaa aaccggcgtt caggtatttc agttcccgga aggcgtcacc tggggtgacg
     1801 gtcaggtagc gtacgtggca atcggtattg ctgccagctc ggataaacat ctgggcctgc
     1861 tacgccagct gacccacgta ctgagcgatg attccgttgc tgaacaactg aagtcagcaa
     1921 caacagcaga agaacttcgc gcattactga tgggcgaaaa gcagagtgag cagctgaagc
     1981 tcgacaacga aatgctgacg ctggatatcg tcgccagcga tctgctgact cttcaggcgc
     2041 tgaacgctgc gcgtctgaaa gaggcgaggg cagttgacgc cactttcgtc accaaagcca
     2101 tcaatgaaca accgctgaat ctcggacagg gtatctggct gagcgatagc gccgaaggca
     2161 atctgcgtag tgcgattgcg gtaagccgtg cggcaaatgc ttttgatgtg gacggcgaaa
     2221 cggcagccat gctggtgagt gtggcgatga atgacgatca gcccatcgcg gttcttaagc
     2281 gtctcgctga tttgttgctc gacaataaag ctgaccgctt gctgaaagcg gatgcggcaa
     2341 cgttgctggc gctgctgacc agcgatgatg cgccgaccga cgacgtgtta agcgcggagt
     2401 ttgtggtgcg caatgaacac ggcctgcatg ctcgtccagg taccatgctg gtcaatacca
     2461 ttaaacaatt taacagtgat attaccgtga caaaccttga tggcaccggc aaaccggcaa
     2521 acggacgtag tctgatgaaa gttgtggcac ttggcgttaa gaaaggtcat cgcctacgct
     2581 ttaccgccca gggtgcagat gctgaacagg cgctgaaagc cattggcgac gctatcgctg
     2641 ctggtcttgg ggagggcgca taatgagcag acgtgttgct acgatcaccc ttaatccggc
     2701 ttatgacctt gttggtttct gcccggaaat tgaacgcggc gaagtgaacc tggtgaaaac
     2761 caccggtctg catgcggcgg gtaaaggcat caacgtggcc aaagtattaa aagacctggg
     2821 aattgatgtc accgttggcg gcttcctggg taaagacaat caggatggtt ttcagcaact
     2881 gttcagcgag ctgggcattg ccaaccgttt ccaggttgta caggggcgca cccgaattaa
     2941 cgttaagctg acggaaaaag acggcgaagt gaccgacttc aacttctcgg gttttgaagt
     3001 cacccccgcc gactgggaac gctttgtgac tgattctctg agctggctcg gtcagttcga
     3061 tatggtctgt gtcagcggaa gcttaccgtc aggcgtcagc ccggaagcgt tcaccgactg
     3121 gatgactcgc ctgcgtagtc agtgtccttg cattatcttt gatagtagcc gtgaagcgtt
     3181 agtagcaggt ttgaaagcgg caccgtggct ggtgaaacct aaccgccgcg agttggaaat
     3241 ctgggcaggc cgtaaactgc ctgaaatgaa agatgtgatt gaagctgcac atgcgctacg
     3301 tgaacaaggt attgcgcatg ttgttatttc actgggtgcc gaaggcgcgc tttgggttaa
     3361 tgcctccggc gaatggatcg ctaaaccacc gtcagtcgat gtcgtaagca ccgttggcgc
     3421 aggggattct atggttggtg gcctgattta tggcttgctg atgcgtgaat ccagtgaaca
     3481 cacactgcgt ctggcgacag ctgttgcagc cctggcggta agtcaaagca atgtgggtat
     3541 taccgatcgt ccgcagttgg ccgcaatgat ggcgcgcgtc gacttacaac cttttaactg
     3601 acagcaggag aggcataatg aaaacgctgc tgattattga cgctaatctc ggtcaggcac
     3661 gcgcctatat ggcgaagacc ctgctgggcg cggcggcgcg aaaagcaaaa ctggaaatca
     3721 tcgacaatcc gaacgacgca gaaatggcga ttgttctcgg tgattccatc ccgaatgaca
     3781 gcgcgctgaa cggtaaaaat gtctggctgg gtgatatttc ccgggcagtt gcgcaccctg
     3841 agctgttcct gagtgaagcc aaaggccatg cgaaacctta cactgcgccg gtcactgcga
     3901 cagcaccggt tgccgccagc ggtccgaaac gcgtagttgc ggtgactgct tgcccgactg
     3961 gcgtagcaca cacctttatg gcggctgaag ccattgaaac cgaagcgaaa aaacgtggct
     4021 ggtgggtgaa agttgaaacc cgtggttctg ttggcgcggg taatgcaatc actcccgagg
     4081 aagtagccgc agcggatctg gtgattgtgg cggcagatat cgaagtggat ctggcgaaat
     4141 ttgctggtaa accgatgtat cgtacctcta ccggtctggc gctgaagaaa acctcgcagg
     4201 aactggataa agcggttgct gaagcaacgc cgtatgaacc ggcgggcaaa gctcaaacgg
     4261 cgaccactga aggtaagaaa gagagtgcag gcgcttatcg tcacttgcta acgggcgtct
     4321 cttacatgct gccgatggtc gttgctggtg gtctgtgtat cgcgctttct tttgcttttg
     4381 gtatcgaagc gtttaaagag ccgggtacgt tggcagcggc gctgatgcag attggtggtg
     4441 gttcagcctt tgcgctgatg gtgccggtac tggcaggtta tattgccttc tccattgccg
     4501 atcgtccggg cctcacgccg ggtctgattg gcggtatgct ggcggtcagc accggttctg
     4561 gcttcattgg cggtattatt gcgggcttcc tggctggtta cattgcgaag ttaatcagta
     4621 cgcaactgaa actgccgcag agtatggagg cgctgaaacc gatcctgatc attccgctaa
     4681 tttccagtct ggtggtcggt ctggcgatga tctacctgat cggtaaacca gttgctggca
     4741 ttctcgaagg gctgactcac tggctgcaga ccatggggac tgcgaatgcg gttctgctgg
     4801 gggcgatcct cggtggcatg atgtgtactg acatgggcgg tccggtaaac aaagcagcgt
     4861 acgcattcgg tgtgggtctg ctgagtactc aaacctatgg cccgatggcg gcgattatgg
     4921 cggcaggtat ggtgccaccg ctggcaatgg gcctggcaac aatggtggcg cgtcgcaaat
     4981 tcgacaaagc gcagcaggaa ggtggcaaag ccgctctggt attggggctg tgcttcattt
     5041 cggaaggtgc aattccgttt gctgctcgtg atccgatgcg tgtgctgccg tgctgtatcg
     5101 tgggtggggc gctgactggc gcaatctcaa tggcgattgg tgcgaaactg atggcaccgc
     5161 acggtggtct gtttgttctg ctgatccctg gcgctattac gccggtattg ggttacctgg
     5221 tagcaattat tgccggtacg ctggtggcgg gtttggccta tgccttcctg aaacgtccgg
     5281 aagtggacgc agtagcgaaa gcagcgtaat aaaaggtgtg ttacagggca gaaatttcct
     5341 gccctgtaac atttgatgag cgcaaagaga atgaccagct tgtgaatgta attttatgaa
     5401 tttaaatggt tttatctttc agtagaaact tcaaacgttc atcaaacact aaggttattt
     5461 cctcaagggc agattatttt ctgccctttt ttagtgttcc tgttctgtgt atcccgtcac
     5521 aaattcctcg cttttgtaat tcaggcattt aatcgcattt tgtgagcctg ctcaaatctg
     5581 cccattttgc ctctctggcg gtaatttact ttacgtaaaa catgcatact atgagcacat
     5641 gtttaaatat gaacacatgt ttaaaccgtc tttgcagtat ctatattctt gtggattgac
     5701 agggttggta attttttacc agaggacgtg ctatgcgcga aaaggattat gtcgtaatta
     5761 taggttcggc gaatattgat gtcgccggat attcacatga atcattaaat tatgtggatt
     5821 caaatccagg taaaataaaa tttacgcctg gtggagtagg gcgcaatatt gcacaaaacc
     5881 tggcgttgct gggtaacaaa gcctggctac tgagcgccgt aggcagtgat ttttatggtc
     5941 aatcgctgct aacgcaaacc aatcaatctg gtgtttatgt cgataaatgc ctgattgtgc
     6001 cgggagaaaa tacgtcgagt tatttatcat tactcgataa taccggtgaa atgctggttg
     6061 ctataaatga catgaatatt agcaacgcta ttacagctga atatctcgca cagcaccgtg
     6121 aatttattca gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa gaggcactgg
     6181 catggattct ggataatgcc gccaacgtac ccgtatttgt cgatccggtt tcagcgtcga
     6241 aatgtgtcaa agtgcgcgac cgtctaaatc agatccacac tctgaagcca aaccgccttg
     6301 aagcggaaac cctgagtggg attgcgctgt cagggcgtga cgatgtggca aaagttgctg
     6361 cctggttcca tcaacatggc ctgaaccgac tggtattgag catgggcggc gacggcgttt
     6421 attacagcga tatcagcggt gaaaatggct ggtctgcgcc gatcaaaacc aatgttatta
     6481 atgttaccgg agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg gtagacggaa
     6541 tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg ctctcctgtg
     6601 aatacaccaa taaccccgat ttatcgattg ccaacgttat atcgttagtg gagaacgcag
     6661 aatgtctgaa ttaaaaattt cccctgaatt attacaaatt tctccggaag tgcaggacgc
     6721 tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcaacc attatttctc acgggatgcc
     6781 gttcccacaa aatgcccaga ccgcaattga agtagaagaa actattcgta aacagggcgc
     6841 agtacctgcc actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaaa
     6901 aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc
     6961 ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg gcttcaacga tgattattgc
     7021 ggcgcttgcc ggaattaaag tatttgccac cgggggaatt ggtggtgtgc atcgcggggc
     7081 ggaacatacc ttcgatattt ctgccgattt gcaagaactg gcaaatacta atgtcaccgt
     7141 tgtttgtgcc ggggcgaaat ctattctcga tttaggatta accactgagt atttagaaac
     7201 cttcggtgtg ccgttaattg gctatcagac taaagcgctg cctgcgtttt tctgccgtac
     7261 cagctcgttt gacgtcagca ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc
     7321 ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta gcgaacccga tcccggaaca
     7381 gtttgcgatg ccggaggaat ctatcaatgc agccatagat caagccgtcg ccgaagccga
     7441 agagcagggc gttattggta aagaaagtac accgttcctg ctggcgcgcg ttgctgagct
     7501 gaccggcggt gacagcctga aatccaatat ccagctggtg ttcaacaacg ccattctggc
     7561 gagtgaaatt gccaaagaat accagcgtct cgcgggttaa tttctgacgg gcagaaagcc
     7621 gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga cgccgggttt actggcatga
     7681 agggaaaatc attatggata taatgagaag tgttgtgggg atggtggtgt tactggcaat
     7741 agcatttctg ttgtcagtga ataaaaagag catcagtttg cgcacggttg gagccgcact
     7801 gctgctgcaa atcgctattg gtggcatcat gctctacttc ccaccgggaa aatgggcagt
     7861 agaacaggcg gcattaggcg ttcataaagt gatgtcttac agtgatgccg gtagcgcctt
     7921 catttttggt tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg cgggttttat
     7981 cttcgccttt cgcgtacttc cggcgattat tttcgttact gcgctcatca gtctgctgta
     8041 ctacattggc gtgatggggc tgctgattcg catccttggc agcattttcc agaaatgccc
     8101 tcaacatcag caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg
     8161 agatcccggc gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg
     8221 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
     8281 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt
     8341 ttgcacgtat tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt
     8401 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
     8461 tgaaaatcgc cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
     8521 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
     8581 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
     8641 ccaaccttgc tggtagcctg attgggcaga aactggcgat taacgaattc atcgcttacc
     8701 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta
     8761 tatcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
     8821 tttcggctat ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
     8881 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
     8941 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
     9001 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
     9061 cgccagtcct gacaactgct ttctgttttt aattagatat cctttcttgc ttttaattaa
     9121 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
     9181 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcttgtg agagtaatat
     9241 aaatgctgcc aggcgattca ttaacggaaa tgattgattc tgagttaaag aaacaatatt
     9301 acgataattt ttatgactta aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa
     9361 taacagcgga cggtaatgtt tcatagggag cgcaaggcac caacactctt caatagcctg
     9421 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcgccaataa aacagggagc
     9481 agcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
     9541 gcctcgcgtc agataaaaca gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg
     9601 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa
     9661 cgcagattca ctcatcaatt gctgcttaag gtcattattg tggatttctt tcacgatgtt
     9721 tatccgctat gagatttctc atatttagca tacgtacgct gttgttaaga ttcaatcatc
     9781 agcccggtcg aattttcttt gctgtagaaa aaaataacgc aactggaaac agaggaaata
     9841 aacaatggaa aagagaaaaa ttattctgga ttgtgagccg ggtcatgatg atgctattgc
     9901 aatgatgatg gcagcgaaac atccggcaat agatttatta ggcatcacta ttgtagcggg
     9961 taatcagacg cttgataaaa cattaattaa tggcctgaat gtttgccaga aactggagat
    10021 taatgttccg gtttatgcgg ggatgccgca acccattatg cgtaaacaaa tcgttgccga
    10081 taatattcac ggtgaaaccg gactggatgg cccggtattc gagccgctga cccgccaggc
    10141 agaaagtacc catgcggtga aatatatcat cgataccctg atggcaagcg atggcgatat
    10201 cactctggtg ccggttggtc cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc
    10261 aatcctgccc aaaatccgtg aaattgtgct aatgggcggc gcttacggta caggcaactt
    10321 caccccatct gccgagttca atatctttgc cgacccggaa gccgcccgcg tggtgttcac
    10381 ctccggcgtt ccattagtga tgatgggcct cgatcttacc aaccagaccg tttgcacccc
    10441 ggatgtaatt gctcggatgg aaagggtagg cggccctgcc ggagagctgt tcagcgacat
    10501 catgaacttc actctcaaaa cgcagttcga atactacggc ctggctggcg gcccggtgca
    10561 tgacgccacc tgcattggtt atctgatcaa ccctgatggc attaaaaccc aggatatgta
    10621 cgtcgaagtg gacgtcaaca gcggcccttg ctacgggcgt acggtctgcg acgaactggg
    10681 cgttcttggc aagcccgcta ataccaaagt cggcatcact attgataccg actggttctg
    10741 gggcttagtc gaagagtgcg tgcgcggcta catcaaaacc cattaagtgt tgataacaag
    10801 ccgggacagt atcgcaagat gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc
    10861 aaaatgacat gaagagataa tcactatgga tgtcatgaga agtgttctgg gaatggtggt
    10921 actgctggca attgcgtttt tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt
    10981 tggcgcggcg ttagtgttac aggtcgtgat tggcgggatt atgctttggt taccgccagg
    11041 gcgttgggtc gcagaaaaag tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc
    11101 aggtagcgca tttatcttcg gttctctggt cggaccgaaa atggatacct tatttgatgg
    11161 cgcaggattt atctttggtt tcagggtatt accggcaatt atcttcgtca ctgcactggt
    11221 gagtattctc tactacatcg gtgtgatggg gatcttaatt cgcattctcg gcggtatctt
    11281 ccagaaagct ttaaatatca gcaagatcga gtcattcgtc gcggtcacca ccattttcct
    11341 cgtgctaaac gaaattccgg cgatcgtcaa accctttatc gatcgtctga atcgcaatga
    11401 attatttaca gcgatttgta gtggcatggc ctcgattgct ggttcgacaa tgattggtta
    11461 cgccgcactg ggcgtacctg tggaatattt gctggcggca tcgttaatgg cgatccctgg
    11521 cgggatcttg tttgcccgcc tgttaagccc ggctacggaa tcttcgcagg tttcttttaa
    11581 taacctctct ttcaccgaaa caccgccaaa aagcattatt gaagccgatg cgacaggggc
    11641 aatgaccggg ctgaaaatcg ccgccggtgt agcgacagtt gttatggcat ttgtcgccat
    11701 cattgcgtta attaacggta ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc
    11761 ctcgctggag tccattttag gttacctgct ggccccactt gcgtgggtaa tgggtgttga
    11821 ctggagtgat gcaaatcttg ccgggagttt gattggacag aaactggcaa taaatgaatt
    11881 tgtcgcttat ctcaatttct caccctatct gcaaacggct ggcactctgg atgctaaaac
    11941 cgtggcgatt atttccttcg cgttgtgcgg tttcgctaac tttggttcta tcggggtggt
    12001 ggtgggggcg ttttctgcgg ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt
    12061 acgcgcgctg gcggcggcga cactttctaa tctgatgagt gccaccattg ccgggttctt
    12121 tattggttta gcgtagtgtg caatttatga gccggattag atgcggtcaa gcgtcgaatc
    12181 cggcaacatt gttacaccat tggcactaat gacagcgcgt tatcggcaga cagggtaggg
    12241 ttgtttaagc tgccgctggc gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg
    12301 tcacggaaat tgtttccctc cagaaagcta tataccagac ccgccataaa accatcgtca
    12361 gcaccaaaac tgtctaccgt cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc
    12421 ttttcgctgc aataaactga ctcatcgggc aaataaacaa acagttgctg aacaccttgc
    12481 tgatgcaatg cattcactgc ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat
    12541 aaaatttcca gctccggtaa agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg
    12601 attttgcccg ctttgaattc tgaaacggta tcgacaaaca ccgggatctc atcagcgagg
    12661 gtaaagaccc attccagcgc ctcggctgtc aggttgcaat caaccagtac cacgcccgca
    12721 tgacgaagta aatcgcgcga cccgttcagt agttgcggtg tcaactgttc cagcagatgg
    12781 gtatcgttaa tcgccagcac ggtttgatcg tctcgattgg caattgccag atacgtcgat
    12841 gtgctttgac catgcaaacg aacgcagccg gagacattca cgccggcacg gcgcgtttct
    12901 tccaggagca tttcgccata aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg
    12961 cctaacagcg ccagattgtg ggcgatattg cgtccaacgc cgcctgccga acaatgaatt
    13021 gtaccgggat gagaagccgc ttgcgggtaa tggatatccg ccatcccgcg aatatccata
    13081 ttgattgtcc ccaccactac gcagtattcc tgctcggtga gaatgtaacc tttgccttta
    13141 atccggcctt tgcgcattaa atccataata tgcgccgcaa cgcgcgaacg actgatttgc
    13201 agcatgtccg caatttcgtt ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc
    13261 tccttttccc ggttattcat cttcaggcta ccgctttttc agtttgttgc gctttcagcc
    13321 aggcgatctc ttctgcccag atatccgggt tgatggtttc aaggatcagc ggaatgccgt
    13381 cgaaacggtc gttctgcata atccagcgga acgcatcatg accgatattg ccttcaccga
    13441 ggctatgatg gcggtcaacg cggctgccaa aggtactttt cgcatcgtta aggtgcatcc
    13501 cgcgcagata cttaaagccg acaatacggg caaaatcagc gaacgttttt tcgcattcgg
    13561 ctggagtacg caaatcatac ccggcagcga aagcatggca ggtatcaatg cagacgccga
    13621 cgcgggattt atcttccacg ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta
    13681 agttactgcc ctgaccggcg gtgttttcaa tcaccgctgt tacaccttga gttttatcca
    13741 gcgcaatgtt gatggattcg gcaatacgcg caaggcaatc ctcttctgaa atctgcatca
    13801 gatggctgcc agggtggaag ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt
    13861 catctataaa ggcatcgcgc gatttttcca gagcttcagt gaccggatgt ccgaggttga
    13921 tcagataact gtcgtgggga agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg
    13981 ctttgaattc atcgatggtt tgcgtcgtga gcggtgcggc acgccactga cgttggtttt
    14041 tggtgaacaa ggcaaacgcg gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca
    14101 gaccgccagc agcactaacg tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc
    14161 cgctggagga aaacggtaat gatagcgggt taacaggagc aagaagtagt ggtttatgcg
    14221 atgacgcttt gaatcacata gttaatcgca ccaccaccaa caatcagcca ggcaaacagt
    14281 accagtgcca tcagcagagg tttcgcccca gcttttttca gcgcgctgac gtgagtggtc
    14341 agacccagcg ccgccatcgc cattgccagc aggaaggtat ccagcgttac cagcatgttc
    14401 accacgctct gcggtaacag gtggaacgag ttaaagatgg caactacgat gaacaagatg
    14461 gcaaaccacg gaatagtgat tttgcttttc tcgccgctgt tcgccccaga cagctgttta
    14521 acacgcgccg ccagcaggat gaggaacgga gccagcatca tcacgcgcag cattttggaa
    14581 ataactgctg cgttttctgc atccgggctg atggcatgac ctgccgccac cacctgcgcc
    14641 acttcgtgca cagtagaacc aatgtagata ccgaaagttt ccggactaaa ccattgagac
    14701 atcagcggat atatcgccgg gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt
    14761 gcaacagcca cggttacttt actggcttcc gctttcacta ccggctcagt cgccagtacc
    14821 gcggcagcac cacagatact gctaccggca ccgatcaacc aactggtgtg cttatccaga
    14881 ccaaacactt tctgccccag gaagcaagcc agcaggaagg tactggacag cgtcagcacg
    14941 tcaatgatga tcccactgat accgacatcg gcaatttgcg agaacgtcag acggaagcca
    15001 taaagaatga tacccagacg taataaatat tgcttggcaa acagcacacc accgtcacag
    15061 cttttccaga tgtgcggata gatggtgttg cctaaaacca tccccaacaa gattgcgagg
    15121 gtgagggcac taaacccggc acccgcaacc gcgggaatgg aaccacccca cagggcgacc
    15181 ccggtgataa ctgcactcag ggctaacccc ggaataaaat gccacagtgt acgatgttgt
    15241 ttctgtaagg tgatattcgt cataaccctc tcctttaacc gggcataagg ttacgactaa
    15301 ctggtttaaa aataaaattg attatatatt tataattaat ctttataagt ggtaagcgac
    15361 tatgcacatc accctccggc agttggaagt ttttgcagaa gtattgaaaa gtggatcaac
    15421 cacccaggcg tcggtgatgc tggcgttgtc gcaatcagca gtgagcgcag ccttgactga
    15481 cctggaaggg cagcttggcg ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa
    15541 tgaacacggg cggctgctct atccgcgagc gttggcactg cttgaacagg cggttgaaat
    15601 cgaacaactg tttcgcgaag acaacggcgc gattcgtatc tatgccagta gtaccatcgg
    15661 taactacatt ctgcctgcag ttatcgcccg ttatcgccat gattatccgc agttgccgat
    15721 tgaacttagc gttgggaaca gccaggacgt gatgcaagcg gtgctggatt tccgcgttga
    15781 tattggcttt attgaaggac cgtgccacag cactgaaatc atttctgaac cgtggctgga
    15841 agacgagctg gtggttttcg ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt
    15901 agaacagctg gccgctgcgc cgtggatcct gcgtgaacgc ggttccggca cgcgggagat
    15961 tgtcgattat ctgttgctgt cacatttacc gaagtttgag atggcgatgg aattaggtaa
    16021 ctccgaggca atcaaacatg cggtgcgtca tgggttggga attagttgcc tgtcgcgacg
    16081 tgtgattgaa gatcaattgc aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg
    16141 cctgatgcgt acgttgtggc gtatacatca tcggcaaaaa cacctttcca acgcgctacg
    16201 gcgctttctg gactattgcg atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca
    16261 tgctggtgct gtgtcgattt cgtgacgcag cgccttcagc atgcattcgc cagaaaagag
    16321 attggctgct ttacttataa tccctgggcg atcatgaagg tgtcttataa ccgtgtattt
    16381 ctgccggaag gattgccaat cgtctgctac aatcgcgcca tcatttttaa gatggatagc
    16441 atttttgtat gggttccgaa actaaaacta cagaagcgcc gggcttacgc cgtgaattaa
    16501 aggcgcgtca cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg
    16561 cctctggcgc aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgctga
    16621 ttggcctgat ggtttacttc ctgatgacca gtctcggtga attggctgca tatatgccgg
    16681 tttccggttc gtttgccact tacggtcaga actatgttga agaaggcttt ggcttcgcgc
    16741 tgggctggaa ctactggtac aactgggcgg tgactattgc cgttgacctg gttgcagcac
    16801 agctggtcat gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc
    16861 tcggcgttat cttcctgttg aactacatct ctgttcgcgg ctttggtgaa gcggaatact
    16921 ggttctcact gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga
    16981 ttatcggtat cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag
    17041 cgccgtttgc tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt
    17101 tccagggaac cgagctgatc ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca
    17161 ttccacgcgc ggtacgtcag gtattctggc gaatcctgtt gttctatgtg ttagcgatcc
    17221 tgattatcag cctgattatt ccgtataccg atccgagcct gctgcgtaac gatgttaaag
    17281 acatcagcgt cagtccgttc accctggtgt tccagcacgc gggtctgctc tctgcggcgg
    17341 cggtgatgaa cgcagttatt ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg
    17401 cgtctactcg tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta
    17461 aattgtcgcg tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt
    17521 tgtgcttcct gacctccatg tttggcaacc agacggtata cctgtggctg ctgaacacct
    17581 ccgggatgac gggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc
    17641 gcggttacgt attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc
    17701 cactggggcc gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg
    17761 aagcgttcct gaaagatacc attgactggg gcggcgtagc ggcaacgtat attggtatcc
    17821 cgctgttcct gattatttgg ttcggctaca agctgattaa aggaactcac ttcgtacgct
    17881 acagcgaaat gaagttcccg cagaacgata agaaataagt tttctccctt ccttgctaag
    17941 ccctctcaac cgagagggct ttttcaattc catttccctg acaaatcatg cggatataaa
    18001 atttaacatt tggattgata attgttatcg tttgcattat cgttacgacg caatcaaaaa
    18061 aggctgacaa atcagaggct gttccggctt tctgggatga tcacctgcat acaaataagt
    18121 ccaccgcgat gctgccgtac gcaaggggac gtaaagaaga tgtgagcgat acccatttta
    18181 ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtcg
    18241 ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg
    18301 aaacgatggt tgtcactgca tcttccgtgg aacaaaatct taaagatgca cccgccagta
    18361 tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc
    18421 tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta
    18481 ttcgtggtct ggacagcagc tacaccctga ttcttgtcga cggtaaacgc gttaactccc
    18541 gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg
    18601 aacgtattga agtggttcgt ggcccgatgt cgtcgctata cggctccgat gcgctcggcg
    18661 gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg
    18721 ataccaccat tcaggaacat cgcgatcgcg gtgataccta taacggtcag ttctttacca
    18781 gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg
    18841 aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaacgccg cgtattgaag
    18901 gattctccag ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt
    18961 ttactgccgg atacggtttc gaccgtcagg atcgtgattc cgactagctg gacaaaaacc
    19021 gcctggaacg ccagaactac tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg
    19081 aactgaaatc ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc agcccgataa
    19141 cttccgaaag caatacggtc gacggcaaat acacgttgcc gctgacggcg attaatcagt
    19201 ttctcacggt tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg aacctgaccg
    19261 ggggaaccag ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa gatgaatggc
    19321 ggatcttcga gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg
    19381 gtgaacactg gagtccgcgt gcctacctgg tttataacgc caccgacacc gtaacggtga
    19441 aagggggctg ggcgacggca tttaaagcac cttctctgtt gcaacttagc cctgactgga
    19501 cgagcaattc ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa
    19561 ccagcgaaag ttgggagctg gggctttact acatgggtga agaaggctgg ctggaagggg
    19621 gtgaatccag cgttatcgtt ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta
    19681 cgtctgacgt caacgctgca ccgggctacc aaaactttgt tggttttgag acgggcgcta
    19741 acggacggcg cataccggta tttagctact acagcgttaa caaagctcgt attcagggcg
    19801 tggaaaccga actgaaaatt ccgttcaacg atgaatggaa actgtcgatc aactacacct
    19861 acaacgatgg tcgtgatgtc agcaacggcg aaaacaaacc gctatccgat ctgccgttcc
    19921 atactgctaa cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg
    19981 tttctggtca ctataccggg cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg
    20041 gttataccat ctggaatacc ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg
    20101 caggcgtgct gaaccttggc gacaaggatc tcagtcgtga cgactacagc tataacgaag
    20161 acggacgtcg ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa
    20221 ccgggcggac tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa
    20281 agaggcgatg aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca
    20341 cgctttctgg cgcgcggctt ctgccaatac cgcaggttgc aactggtcgg caagaaactg
    20401 atcattatcg ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat
    20461 cagcgcgcaa ctatcccatt ccagccatgc atttttgtcc tcacctaaat aggtgctaaa
    20521 cgctttgatt ccccacggga cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct
    20581 ggtgtattta cccggatttt tcagcgccat aatcagcgca ccgtgaccac ccattgagtg
    20641 accgctaatg gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc
    20701 atcgcgcaga taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag
    20761 ataaaagcct gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg
    20821 cgggctggtg tctggcatca ccagtacaat ccccagttcc gccgctaccc gctgggcacc
    20881 cgccttggtg gtgaagttct cgtcattgca ggttaatccg gaaagccagt acagcactgg
    20941 tggcggggtg tgatcacgag ggggtgggag aaagatactg aacgtcatcg ggcagtttaa
    21001 ggtactggag tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag
    21061 catttccatg cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc
    21121 aatgtgctgt gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga
    21181 tgaaaggcag cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct
    21241 cacgcacaat aatcaagctg taaatcagct taataacttt gcccccacgc agggcggagg
    21301 cgtcacacct gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc
    21361 atgaagcgtt agttgcgaga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg
    21421 ataacgaaac gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga
    21481 atctcgacct ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg
    21541 tcgatgaaat tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa
    21601 acaaaatgaa ggtcgatgaa atggtcaccg tgcgcgatat cactctgacc agtacctgtg
    21661 aacaccattt tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg
    21721 tgatcggtct gtcaaaaatt aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc
    21781 aggaacgtct gacgcagcaa attcttattg cgctacaaac gctgctgggc accaataacg
    21841 tggctgtctc gatcgacgcg gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa
    21901 ccagtgccac gacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc
    21961 acgagtttct gcgcgctgtg cgccatcaca actgattaaa aggcaggaac catggagcgc
    22021 aacgtcacgc tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc
    22081 agcgcctttg ggctaccaaa ggcggcttat ctcaatcccg cctggtacga tgctattacg
    22141 ccgcaggatg catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg
    22201 ctttttgcgc tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg
    22261 atccagtcgc ggttaacgct gttagtcttg ttgggcttta ttcacggttt attgttctgg
    22321 gacggcgata ttctgctggc ttacgggctg gtgggcttaa tctgctggtg gctggtgcgc
    22381 gatgcgccat cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt
    22441 ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc ctggacgccg
    22501 gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc
    22561 agtaatcgtg ccgatggtgt tggcaatagt ttactggcac tgggcgcaca gtatggctgg
    22621 caactggcag ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg
    22681 cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc
    22741 attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc
    22801 ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg
    22861 ctgttttatg gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc
    22921 gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt
    22981 taccacctcg gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg
    23041 gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg
    23101 gtggaatggc tctggcgtca gttaactttg cgtgctgccg gactggcaat atctaaaaca
    23161 tcaagataac gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg
    23221 tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca
    23281 tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag
    23341 gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc
    23401 gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat
    23461 gcccaggcgc tggcaactca ggttagcgac accattggcg tggtggtgat ggacgtttct
    23521 gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa
    23581 tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg
    23641 ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag tgacgatgaa
    23701 ctggcacaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg
    23761 tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg
    23821 ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg cattgaagat
    23881 gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg
    23941 gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc gatggttgaa
    24001 ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct
    24061 ggcgcactga cagcattaaa agataatggc attgcgattc cgttacatct ctcaatcatc
    24121 ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac cgtgcgttat
    24181 cccattgctt caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat
    24241 attgatcctc gtgccgccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa
    24301 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
    24361 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaatg
    24421 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
    24481 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
    24541 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
    24601 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
    24661 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
    24721 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg
    24781 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
    24841 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
    24901 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
    24961 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
    25021 ttggcactga ctccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
    25081 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcagttcgta ctgctgaaag
    25141 gtgaaccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gaattgaacg
    25201 ataaaggcat caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg
    25261 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
    25321 tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt
    25381 ccagaattcc ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg
    25441 gtgcactggc gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    25501 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    25561 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacttggct gaattcagca
    25621 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    25681 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    25741 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    25801 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    25861 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    25921 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    25981 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    26041 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    26101 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    26161 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    26221 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    26281 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    26341 aagttaccgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    26401 tggacaagat catcggcatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    26461 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    26521 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    26581 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    26641 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    26701 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    26761 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    26821 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    26881 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    26941 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    27001 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    27061 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    27121 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    27181 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    27241 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    27301 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    27361 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    27421 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    27481 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    27541 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    27601 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    27661 atggcatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    27721 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    27781 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    27841 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    27901 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    27961 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    28021 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    28081 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    28141 tcggcgtaaa cccatactgg cagtacatta tcaaaggggc gattattatc ttcgccgtag
    28201 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgcggatat tgccggatgc
    28261 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    28321 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    28381 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt
    28441 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    28501 acagccaaca catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    28561 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc
    28621 ctgatcggcg agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta
    28681 ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc
    28741 atcttcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    28801 ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    28861 ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    28921 ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc
    28981 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    29041 ggaaccccgt ttcactgccc gacaatattt ttttaccagc tccgggcttt gcccgacatc
    29101 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    29161 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    29221 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt
    29281 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    29341 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    29401 tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    29461 gcctaccggc gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat
    29521 cgaaagatct ttcgttaaca tgagcaagct ccctctaata tagtgatgaa tcgcctcggc
    29581 ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa caatatcgcc
    29641 agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg tgttacgctg
    29701 ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt
    29761 atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga ctttattgcc
    29821 ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggc
    29881 aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc aagttaccgc
    29941 ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat
    30001 aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtacac gttgcaggaa
    30061 gtcgacggca atctcaacgg tcgctatgct caaacagcgg tagcccggaa ccgcttgata
    30121 accccacggt gaccagtacc gcgcggtttt ccgctttcag ttgctccagg gtgagtgttt
    30181 taccgatttc gttattgcac ttaatggtca cgcccatttt ttcaatacgg gcgatctctg
    30241 catccagcac tgattgcggt aaccggaatt gcggaatacc gttacgcaac cagccaccgg
    30301 ggtgcgcttc tttctcataa atcgtgacgt cataaccctg gtttgtcagt gtcacactgg
    30361 cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt ttagtaccgg
    30421 gctgataaat ttccatcccg gtttgttgtt caaaatcagt aacaaaacgc tgtaagcggc
    30481 cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat aatttctccg
    30541 tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt gtctcggcag
    30601 cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg gtctgtgccg
    30661 ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct tctttaatcg
    30721 ccagtaacgg cgtaaaagcc ggagtgagtt catccagata attttgttgc ggcatgtgat
    30781 cttccttaat gctatgagta attatggcga acgctaaata aatattgcgt gctgatatta
    30841 aggctatgcg cgtggggaaa tttaaactgt gaaaggattc acaaatagct gcggttcaga
    30901 atgttatatc cgcagcttat tgagtgaagt aataattatt ctcaataatt aacagaaaag
    30961 agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
    31021 ccaatatatg ccaccatata agcgaagaag agtccgggat tttttgctcg ccagacatac
    31081 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
    31141 gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt
    31201 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtaacgg
    31261 cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc
    31321 ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
    31381 caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag
    31441 cctgaatccc catatgcagc gcaataaata atgctcgctc acagtggaaa cgttgggtga
    31501 taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac
    31561 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
    31621 tgcgcatggt catcggctcg ttataacttt gcaatgcgtt atcgccgctc agtaatagat
    31681 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
    31741 gattaataac gccggtacga taatattttg ctgtgccgag caccacgccg acctggcggt
    31801 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
    31861 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
    31921 gctttaacat gcaggagact cgagctaagg aaaatttcca tcaggctact ttacccgctg
    31981 gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc
    32041 aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcacgcggtc
    32101 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
    32161 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
    32221 attcaacaga attaacgccc cctgcaacgg cggcagagtc gggttgaatg cggcgttttc
    32281 agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggtga
    32341 cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc
    32401 accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc
    32461 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcaa
    32521 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
    32581 aggcgtatag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
    32641 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt gcgccaataa attccatatt
    32701 ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
    32761 caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc
    32821 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
    32881 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
    32941 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
    33001 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acacgtgaaa
    33061 cttacatcaa aaatgcgcga cgtatcgcaa attttagccc attactgcca gaataatcgg
    33121 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact
    33181 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
    33241 agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
    33301 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
    33361 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
    33421 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
    33481 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
    33541 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
    33601 gtttagcact tcgctacctt taaagtaact gtcgtaagag atgccggtca gcattaaaaa
    33661 aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc
    33721 cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt
    33781 cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgt
    33841 gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga aaccactacc
    33901 gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
    33961 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca tttcgccgga
    34021 agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg
    34081 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
    34141 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
    34201 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
    34261 acgcttattt tttcgctacc cggtattacg caatgagcag cgaactcagg catacatcaa
    34321 gcatagagag ggattgcagt ttgcagcaat ccctctttgc agacctttaa tccgctgacc
    34381 ataggcgcta acatagcgcc tcattttttt gcgggtgatg atatacgtgt gttactggca
    34441 ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac
    34501 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt
    34561 catcgcattt gctctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc
    34621 gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag
    34681 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt taacggtagc
    34741 ggcggcgggg cgacgttact caaagatcct gaactcatct accagggggc aaaagcgatg
    34801 cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag tgcgtctggg ctgggacagc
    34861 ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggcgctac ggagctggtg
    34921 gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
    34981 ggcgagattc gccagcggct gaatattccg gtgattgcca acggtgaaat ctgggactgg
    35041 cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
    35101 gcgctcaata ttcccaacct gagccgggtg gtaaaatata acgaaccgcg aatgccgtgg
    35161 ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg
    35221 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
    35281 gcaacggaat tatttcagca tgttcg
//
LOCUS       DADLTI010000022        34804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLTI010000022 DADLTI010000000
VERSION     DADLTI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.022"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(18..93)
                     /locus_tag="JN755_000993"
     tRNA            complement(18..93)
                     /locus_tag="JN755_000993"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JN755_000994"
     tRNA            complement(152..228)
                     /locus_tag="JN755_000994"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JN755_000995"
     CDS             complement(331..1716)
                     /locus_tag="JN755_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7379872.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN755_000996"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN755_000996"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7379873.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN755_000997"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN755_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7379874.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JN755_000998"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JN755_000998"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7379875.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JN755_000999"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JN755_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7379876.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /locus_tag="JN755_001000"
     CDS             complement(6327..7457)
                     /locus_tag="JN755_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY7379877.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JN755_001001"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JN755_001001"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7379878.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JN755_001002"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JN755_001002"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7379879.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JN755_001003"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JN755_001003"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7379880.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JN755_001004"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JN755_001004"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7379881.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JN755_001005"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JN755_001005"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7379882.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JN755_001006"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JN755_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7379883.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JN755_001007"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JN755_001007"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7379884.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JN755_001008"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JN755_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7379885.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JN755_001009"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JN755_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7379886.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JN755_001010"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JN755_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7379887.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JN755_001011"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JN755_001011"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7379888.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JN755_001012"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JN755_001012"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7379889.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JN755_001013"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JN755_001013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7379890.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="JN755_001014"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="JN755_001014"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7379891.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JN755_001015"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JN755_001015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7379892.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="JN755_001016"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="JN755_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7379893.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JN755_001017"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JN755_001017"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7379894.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JN755_001018"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JN755_001018"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7379895.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JN755_001019"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JN755_001019"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7379896.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JN755_001020"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JN755_001020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7379897.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="JN755_001021"
     CDS             complement(29521..31167)
                     /locus_tag="JN755_001021"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7379898.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="JN755_001022"
     CDS             complement(31170..31220)
                     /locus_tag="JN755_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379899.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="JN755_001023"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="JN755_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7379900.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33276
                     /locus_tag="JN755_001024"
     CDS             31756..33276
                     /locus_tag="JN755_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY7379901.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="JN755_001025"
     CDS             complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="JN755_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7379902.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33758..34597
                     /gene="hdfR"
                     /locus_tag="JN755_001026"
     CDS             33758..34597
                     /gene="hdfR"
                     /locus_tag="JN755_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7379903.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34692..34767)
                     /locus_tag="JN755_001027"
     tRNA            complement(34692..34767)
                     /locus_tag="JN755_001027"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34732..34734),aa:Trp,seq:cca)
BASE COUNT         8486 a         9521 c         8794 g         8003 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt atgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccaa ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcacgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcataa
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccaa
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacaa taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagtcaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttcagccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttaccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc tcgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttac
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc atggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacaatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttctccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtggtg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggag cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggctattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgctggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc gatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggagtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcgca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catagcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgt tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ctattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca accggctctc ggggcccttt
    32881 ctcgaccgct tcgatctctc actggagatc ccattaccac cccccggcat tttgagtaaa
    32941 acggtagtgc cgggagaaag cagcgccacc gttaaacaac gcgtaatggc cgccagagag
    33001 cgccaattta agcggcagaa taaactgaat gcctggctgg atagtccgga aatacgccaa
    33061 ttctgcaaac ttgagagcga agatgcgatg tggttggaag aaacgctgat ccatctgggg
    33121 ttatcgattc gtgcctggca gcggttactg aaagttgcac gaaccattgc tgatattgat
    33181 cagtctgaca ttatcacacg tcagcatttg caggaggcag ttagctatcg tgcgattgac
    33241 cgtttgctca tccatctgca aaaactactg acataaaaaa agggcatttc gcccttttta
    33301 ttaatcgtca gaatcggtgt agtcttcagc accttcaacc tgcggtttac cgccggaaag
    33361 ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac ttggaccaca cgcgttctgc
    33421 ttctgtcact ggctcacgtt cgccacggca tactgctacg aagagtttct cttcctcggt
    33481 aaccggctcg cgtttgccaa gatccaactc attgaaggca taaccatgac gctcaagcag
    33541 ttgtgcctct ttgatggtga aatcaccatg acgagagaat ccacgtggat aatgtttatt
    33601 gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt
    33661 gaccgagcta gttatggcgc ggagtattag ttacgcttga cagagtgtaa aacaaaacat
    33721 ttaaatcata acgacaaata attttgcgga gagcactgtg gatacggaat tgttaaaaac
    33781 tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac
    33841 ccagtcagca gtgagctttc gaatcagaca actggaaaat caactgggtg tgaacctttt
    33901 cacccgccac agaaacaata tccgtttaac cgctgccggt gaaaaactac tgccttatgc
    33961 agaaacgctc atgagcacct ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca
    34021 taacgagttt tctatcggtg ccagcgcctc gttgtgggaa tgtatgctta atcagtggtt
    34081 gggacgcttg tatcaaaatc aggatgccca tacaggctta cagttcgaag cgcgaattgc
    34141 ccaacggcag tctctggtaa aacagctgca tgaacgccag cttgatcttc ttattaccac
    34201 tgaagcgccc aaaatggacg aatttagtag tcagttgctg gggtatttca ctttagcgct
    34261 ttataccagt gccccttcta aactaaaggg agatcttaac tatctgcgac tggagtgggg
    34321 gccagatttt caacagcatg aggcaggttt gatcggtgct gacgaagttc ccattctgac
    34381 aaccagttct gctgaactgg cacagcaaca gattgcgatg cttaatggtt gcacctggct
    34441 acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc gttgtcgata gcacaacact
    34501 ttcacggccg ctttatgcca tatggctgca aaatagcgat aaaaatgcgt tgattcgcga
    34561 tcttttgaaa attaacgtgc tggatgaagt gtattaatct gaatggctgg caaggatgcc
    34621 ggtagaagga tttacttcgg agaggattat ttcagataaa aaaatcctta gctttcgcta
    34681 aggatgattt ctggcagggg cggagagact cgaactccca acacccggtt ttggagaccg
    34741 gtgctctacc aattgaacta cgcccctaat tagggtggcg gaacggacgg gactcgaacc
    34801 cgcg
//
LOCUS       DADLTI010000023        34550 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLTI010000023 DADLTI010000000
VERSION     DADLTI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.023"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..445
                     /locus_tag="JN755_001028"
                     /pseudo
     CDS             1..445
                     /locus_tag="JN755_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            515..1291
                     /gene="yaaA"
                     /locus_tag="JN755_001029"
     CDS             515..1291
                     /gene="yaaA"
                     /locus_tag="JN755_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7379904.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(1444..1740)
                     /locus_tag="JN755_001030"
     CDS             complement(1444..1740)
                     /locus_tag="JN755_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7379905.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1954..3240)
                     /gene="thrC"
                     /locus_tag="JN755_001031"
     CDS             complement(1954..3240)
                     /gene="thrC"
                     /locus_tag="JN755_001031"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7379906.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(3241..4173)
                     /gene="thrB"
                     /locus_tag="JN755_001032"
     CDS             complement(3241..4173)
                     /gene="thrB"
                     /locus_tag="JN755_001032"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7379907.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4175..6637)
                     /gene="thrA"
                     /locus_tag="JN755_001033"
     CDS             complement(4175..6637)
                     /gene="thrA"
                     /locus_tag="JN755_001033"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7379908.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(6718..6783)
                     /gene="thrL"
                     /locus_tag="JN755_001034"
     CDS             complement(6718..6783)
                     /gene="thrL"
                     /locus_tag="JN755_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7379909.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6997..7683)
                     /locus_tag="JN755_001035"
     CDS             complement(6997..7683)
                     /locus_tag="JN755_001035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7379910.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8083..8223)
                     /locus_tag="JN755_001036"
     CDS             complement(8083..8223)
                     /locus_tag="JN755_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379911.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8319..9035
                     /gene="arcA"
                     /locus_tag="JN755_001037"
     CDS             8319..9035
                     /gene="arcA"
                     /locus_tag="JN755_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7379912.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9094..10446)
                     /gene="creD"
                     /locus_tag="JN755_001038"
     CDS             complement(9094..10446)
                     /gene="creD"
                     /locus_tag="JN755_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7379913.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10504..11928)
                     /gene="creC"
                     /locus_tag="JN755_001039"
     CDS             complement(10504..11928)
                     /gene="creC"
                     /locus_tag="JN755_001039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7379914.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(11928..12617)
                     /gene="creB"
                     /locus_tag="JN755_001040"
     CDS             complement(11928..12617)
                     /gene="creB"
                     /locus_tag="JN755_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7379915.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12630..13103)
                     /gene="creA"
                     /locus_tag="JN755_001041"
     CDS             complement(12630..13103)
                     /gene="creA"
                     /locus_tag="JN755_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7379916.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13314..14183
                     /gene="robA"
                     /locus_tag="JN755_001042"
     CDS             13314..14183
                     /gene="robA"
                     /locus_tag="JN755_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7379917.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14180..14827)
                     /gene="gpmB"
                     /locus_tag="JN755_001043"
     CDS             complement(14180..14827)
                     /gene="gpmB"
                     /locus_tag="JN755_001043"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7379918.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14879..15400
                     /gene="yjjX"
                     /locus_tag="JN755_001044"
     CDS             14879..15400
                     /gene="yjjX"
                     /locus_tag="JN755_001044"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7379919.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15485..15811)
                     /gene="trpR"
                     /locus_tag="JN755_001045"
     CDS             complement(15485..15811)
                     /gene="trpR"
                     /locus_tag="JN755_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7379920.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15901..17838)
                     /gene="sltY"
                     /locus_tag="JN755_001046"
     CDS             complement(15901..17838)
                     /gene="sltY"
                     /locus_tag="JN755_001046"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7379921.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18049..19716
                     /gene="ettA"
                     /locus_tag="JN755_001047"
     CDS             18049..19716
                     /gene="ettA"
                     /locus_tag="JN755_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7379922.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20024..21256)
                     /gene="nadR"
                     /locus_tag="JN755_001048"
     CDS             complement(20024..21256)
                     /gene="nadR"
                     /locus_tag="JN755_001048"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7379923.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(21277..22659)
                     /gene="radA"
                     /locus_tag="JN755_001049"
     CDS             complement(21277..22659)
                     /gene="radA"
                     /locus_tag="JN755_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7379924.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22708..23676)
                     /gene="serB"
                     /locus_tag="JN755_001050"
     CDS             complement(22708..23676)
                     /gene="serB"
                     /locus_tag="JN755_001050"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7379925.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23782..24426
                     /locus_tag="JN755_001051"
     CDS             23782..24426
                     /locus_tag="JN755_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7379926.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24454..25470
                     /gene="lplA"
                     /locus_tag="JN755_001052"
                     /pseudo
     CDS             24454..25470
                     /gene="lplA"
                     /locus_tag="JN755_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            25502..25765
                     /locus_tag="JN755_001053"
     CDS             25502..25765
                     /locus_tag="JN755_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7379927.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(25926..26645)
                     /gene="deoD"
                     /locus_tag="JN755_001054"
     CDS             complement(25926..26645)
                     /gene="deoD"
                     /locus_tag="JN755_001054"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7379928.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26702..27925)
                     /gene="deoB"
                     /locus_tag="JN755_001055"
     CDS             complement(26702..27925)
                     /gene="deoB"
                     /locus_tag="JN755_001055"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7379929.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(27977..29299)
                     /gene="deoA"
                     /locus_tag="JN755_001056"
     CDS             complement(27977..29299)
                     /gene="deoA"
                     /locus_tag="JN755_001056"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7379930.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(29426..30205)
                     /gene="deoC"
                     /locus_tag="JN755_001057"
     CDS             complement(29426..30205)
                     /gene="deoC"
                     /locus_tag="JN755_001057"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7379931.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30463..32004
                     /locus_tag="JN755_001058"
     CDS             30463..32004
                     /locus_tag="JN755_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7379932.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            31976..32839
                     /gene="yjjW"
                     /locus_tag="JN755_001059"
     CDS             31976..32839
                     /gene="yjjW"
                     /locus_tag="JN755_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7379933.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33053..33835)
                     /locus_tag="JN755_001060"
     CDS             complement(33053..33835)
                     /locus_tag="JN755_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7379934.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(33832..>34550)
                     /locus_tag="JN755_001061"
     CDS             complement(33832..>34550)
                     /locus_tag="JN755_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7379935.1"
                     /translation="QMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVIRAS
                     SAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQWFK
                     RMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIALGSR
                     IPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVANDTLV
                     AEVSDAPQANDPTFNNEDLA"
BASE COUNT         8210 a         9526 c         8702 g         8112 t
ORIGIN      
        1 gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc
       61 tgaatttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat
      121 ttatgccgaa aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt
      181 gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg
      241 gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg ccattttact
      301 gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt
      361 gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctccggacgc
      421 gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc
      481 taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa
      541 aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga
      601 caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct
      661 gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc
      721 agatttcacg ccggcgaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac
      781 cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg
      841 aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct
      901 ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga
      961 tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa
     1021 tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat
     1081 caagccggtg ttcctcgatg agaagaacgg caaatttaag atcatcagct tctacgctaa
     1141 gaaagcacgc ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca
     1201 actgactggt tttaatagcg aaggttactt ctttgatgaa gattcctcca gcaatggcga
     1261 actggtgttt aaacgctacg agcagcgtta agtttgtagt caatgccgga tgcggcgtga
     1321 acgccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt aacgtaggcc
     1381 tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt
     1441 catttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt
     1501 atggtggcgc ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata
     1561 atgttgtttc caccagccgt ggtcgcgcca gtgacctcca tcccagtaat agccacgatt
     1621 atcacgatcg cctatctgta attttactga cgggactaac gtaatttccg cagcctgtgc
     1681 tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
     1741 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
     1801 cgacggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gataatttct
     1861 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
     1921 caaacccggc ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg
     1981 caacgcagaa aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc
     2041 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
     2101 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
     2161 cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg
     2221 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
     2281 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
     2341 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
     2401 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgaatga
     2461 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
     2521 ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
     2581 cagcaaacgg ctgatgttaa tcgagttagc cgagtttaac cccagcgcca ctttcagttc
     2641 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
     2701 aaccgtttcg atattgccgc ccaacgtaca gaacagtttt tcttgcagtg gactgatttt
     2761 gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac
     2821 tgcagctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat catccgcaat
     2881 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
     2941 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
     3001 aaacgccgcg cgcacgcgct cttccaggat ttcctgtggg atttcatcac caataaacgc
     3061 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
     3121 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
     3181 ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt agagtttcat
     3241 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
     3301 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
     3361 gtcacataga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
     3421 ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc
     3481 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
     3541 aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
     3601 taaaatagcc cgggcttctg ccgtcgagac tttaattccc ggatacgcca gcacccacag
     3661 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
     3721 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc
     3781 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cgcagtgttc
     3841 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
     3901 cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
     3961 acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa
     4021 gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
     4081 caatgcacca tcaacgggtg ccaccgccgc cccgagcaca tcaaacccga cgctcatatt
     4141 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
     4201 tacgtagcag atcggcaaag acaccggcag cggtaacgtc attgcccgca ccatatccgc
     4261 gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat
     4321 ttttcacttt gaacagcgga tcattcccat ccacttcggc aatcttcacg cggcagacac
     4381 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
     4441 cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac
     4501 cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
     4561 cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat
     4621 catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagcgtggtc gcctcggaga
     4681 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
     4741 agaacttcac caattcatca cctgcattga gcagattttg caggttctca ataaccggta
     4801 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac
     4861 gcaactgatg atagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt
     4921 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
     4981 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
     5041 taaacggctc tttggcttgt gcaagttctt cctgccagtt ttccagatta aggccatgca
     5101 cactagtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat
     5161 tcttcagcca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
     5221 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
     5281 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
     5341 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
     5401 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
     5461 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
     5521 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
     5581 atgattgcgt aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg
     5641 ccgccatgcc aaccatccct ttcatccccg ggccggaaac gctgaacatt gccatgttat
     5701 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
     5761 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
     5821 tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
     5881 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
     5941 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
     6001 ccgccgcgga gtagtcggaa ccgttacgtc caagcaccac cagttcgcct ttttcattac
     6061 cggcggtgaa acctgccatc aacaccatgt gatcagccgg aatgcggctt gccgcaatac
     6121 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
     6181 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
     6241 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
     6301 gccccaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc tgatcgacga
     6361 aagttttcaa ttgcgccagc gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt
     6421 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
     6481 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca
     6541 cctgcccctg cctggcattg ctttccagaa tatcggcaac acgcagaaaa cgttctgcat
     6601 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
     6661 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
     6721 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
     6781 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
     6841 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
     6901 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
     6961 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
     7021 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
     7081 ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgccat
     7141 gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
     7201 ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc ccagattgag
     7261 cgaaggataa tccgccacca tcggcacacc agtaagcacg tcagccaacg ctaactcttc
     7321 gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
     7381 tttttcctct aacagcggca ccagttctac tggcgtggcg tagtaatgat atttcgcccg
     7441 actacgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg
     7501 gaaaacttta atattatcaa taatatcact agatccatgt gcgacccagc gggtggctgg
     7561 ctccaggtgt gcctgactat cgacaatccg cagttcgcta aaacccatcg ttttcattgc
     7621 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accaggataa tcgttatacg
     7681 catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcactgt caacttatta
     7741 cgcggtgcga atttacaaat tcttaacgta agccgcagaa aaagcccttt acttagctta
     7801 aaaaaggcta aactattttc tgactgcact aacggtttaa ttgttaaaaa atgctacata
     7861 tccttctgtt tacttaggat aattttataa aaaataaatc tcgacaattg gattcaccac
     7921 gtttattagt tgtatgatgc aactagttgg attattaaaa taatgtgacg aaagctagca
     7981 tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg atatatgtca
     8041 acgaagcgta gttttattgg gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat
     8101 ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac ccacgaccaa
     8161 gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac gtactttagt
     8221 catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg acttttgtac
     8281 ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg cacattctta
     8341 tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa gcggaaggct
     8401 atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct gaatatgaca
     8461 tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg
     8521 aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac aacgaagtcg
     8581 ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg ttcaacccgc
     8641 gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac catgaatctg ggtactgtca
     8701 gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg gacatcaaca
     8761 gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc gagttccgcg
     8821 ccatgcttca cttctgtgaa aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga
     8881 aaatgaccgg ccgtgagctg aaaccgcacg accgtactgt agacgtgacg atccgccgta
     8941 ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc attcacggtg
     9001 aaggttatcg cttctgcggt gatctggaag attaatcggc tttaccaccg tcaaaaaaac
     9061 ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta attcatcgtc
     9121 cgttgtaact tctttactgg ctttcatttt cggcagtgaa aacgcatacc agtcgatatt
     9181 acgagtcaca aacatcatgc cggccagcgc caccaccagc acactggttc ccaacaacag
     9241 cgcgctatcg gcagagttga gcagtcccca catcactcca tccagcaaca acagcgcgag
     9301 ggtaaacaac atgctattgc gccagccttt caataccgcc tgcaaataaa taccgttcat
     9361 taacgcccca atcagactgg cgattatcca tgccacggta aaaccgatat gttcagaaag
     9421 cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat attgcattgg
     9481 gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat
     9541 cagcagaatg gcgtacttag tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt
     9601 cactgcgacg ctaaacgcgg ggaagttttc ccagccggta tcattgcctg aagcaaaacg
     9661 ctcaccgaga ttattagcaa accagctgct ttgccattgc gcctgaaaac ctgactcgct
     9721 agcttcccgt ttggctggta gaaaatcacc taaaaaactg ggatgcggcc agttgctggt
     9781 taaggtcatt tcgctattac gcccggcagg caccacagaa agatcgccgg taccgcttaa
     9841 attcagtgcc atattcagct gcaggttctg cttccgccag tccccttcgg gtaaagggat
     9901 atgcacgccc tgcccgcctt gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc
     9961 gttaacttca ggcgctttca ccacaccaat accacgcgca tccccgacgc taatcacaat
    10021 aaagggcttg cctaagatga tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa
    10081 atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc ctatcttgcg
    10141 ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa aatggataaa
    10201 acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct cggtcacagg
    10261 aatagcgata agcggcccaa cgagtttttg cggcccgctg gtactttggc gaatcgcatc
    10321 ttccacatcg ctacggtaat cggcacgttc gacaatcact tgccgaatca gcattatcgg
    10381 aatcaacaac agcaatactg caccaaacaa ggtggtcatt ttccagaaca ggggggattt
    10441 caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg gaagaatttg
    10501 aagctatgtg aagttacggt gaagtcgtag cgaggccagc acgccacctt cctgcacgtt
    10561 gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg ccaaccccag
    10621 accgctgctt ttttgcccat ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga
    10681 cagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagcgtga cgtgttcctg
    10741 atccacttcg gcgcttagcg ttatgcaacc gctctcggga gtaaaatcga tggtgttatc
    10801 cagtaaattc cccagcgcct gatccagtaa cgccggttca gcagcaacat taacctcggt
    10861 gggcataaca tgcaaagtga tgtttttttc tgccaactgc acggtgcgcg cttcgctgac
    10921 gcggcgaaat aatgccgcca catcaacagc agtcagaacg acttcctgac gattctccag
    10981 tcttgcctgg cgtagtaacg tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat
    11041 gttgtcagta aaacgagcca ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc
    11101 gccacgaatc gccgccagtg ggctttttag ctcatgggtt aacgcataaa catactgctc
    11161 aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg cgagtttacg
    11221 cagctcgcta ctaccgagat cgggaagagg aacgggctta ttgtcagtga cggaatcagc
    11281 atagcgagtg agccgggcaa tggagcggtt gatccaccaa accatgcctg cgccaatcac
    11341 cagtgcaatc cccaacaaaa tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat
    11401 gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc
    11461 gcccataatc ggcgcggcaa catacatcac cgaactttcg ggatcggcag gattttgcaa
    11521 cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc agcgcgaata
    11581 atcctgtcca acggctttat ttgccgaatc gaacaatact ttgccatgcg catcggtcat
    11641 atagacatga tattcattgc gcactttgtt aatgccacca atattggcgc gaaacgggcg
    11701 atgttgtagc tgattaaacg cctgcgccag ttgcccatgc gttgggtccc cagagagcaa
    11761 atcgggacgc gccagctccg caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc
    11821 tcttcgcacg cccggtttaa cttctttgac aaaaatagcc agtacgaacc aggccgccac
    11881 cgccaccagt aaaaaatagc ccagcagcaa ccgcatgccg atacgcatta caagcccctc
    11941 aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt gatggcgcgc
    12001 agcttggcac gcagcgtttt aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca
    12061 tcttcccaca cgctatccat tagttgctgg cgggaccaga cgcggcctag cgacttgagt
    12121 aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg
    12181 atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg agacggcgtc
    12241 gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa
    12301 aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg atcgacctct
    12361 tcacttcggg ccgtcaggaa cagtacaggt aacgccggat ggagcgccag taactggcgg
    12421 cacaattcaa agccactgat atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc
    12481 tgctggcgag ctttatccag caccggcaaa cctcgctcaa agacctcgac ggcaaaacct
    12541 tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc cactaaccag
    12601 accgtttccc gttgcataaa tcgcctctgt tattgccgcc acggcatgac aggaaccgcg
    12661 ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag ataagcgagc
    12721 gcgttgcgtt tggcgtcata aaagcgcacg acctgtaacg acttaaagac cagggacgtg
    12781 cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc cgacagttca
    12841 atcggcccga cttgctgaca agaaatggcc gcatcggagg tatcttctgc caggcccaat
    12901 ccccctttaa taccaccggt tttcgcccgg ctcacataac aggtgacatt tttcacatcg
    12961 ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt aaatacggtg
    13021 tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag gcttaaagac
    13081 aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg agcaattagt
    13141 tcgtcacggt aaataacata ttgattaaga acaaaatctc aattctttta tttccgtcag
    13201 gccctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta
    13261 ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat tttatggatc
    13321 aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat cagcccctgt
    13381 cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag agaatgttta
    13441 aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg
    13501 cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg
    13561 actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact cctgcacttt
    13621 accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctacgt ctgggtaaat
    13681 tcacaatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt ggtgttaccc
    13741 agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg cgttatcagt
    13801 tctggcacga ttttctcggc aacgcgccga ccattccgcc ggtgctctac ggcctgaatg
    13861 aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt attctatacc accgcgttag
    13921 cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg cagggcggcg
    13981 aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg
    14041 tatacggaac gtgcatgcca atgctcaatc tgacgcgccg taaaggtcag gatattgagc
    14101 gatactaccc ggcagaagac gccaaagcgg gagatcgccc aattaatctg cgctgtgaac
    14161 tgctgattcc tatccgccgt taacgctgca gctcatctaa tgcaggggcg tccagatgcg
    14221 agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt tcctgataat
    14281 ccacgcgcga aatagaacag ttacgcagac gtaagcggcg ctctgcccat gctggtaatc
    14341 cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc agcggtcggc
    14401 ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg ctgagctcct
    14461 gcattgactc gccttcagga atacgcccgt caacggtgcc attgaccagc tgccgacgcc
    14521 agttctcttc ttcttcggtc agagaatcga tatgtctttt ttccagcaca cccatgttta
    14581 attcacgcag gcgagaatca aagatgatgt cacagccgca ggcctgggcg atgatttccg
    14641 ccgtacgccg ggtgcgtcct aaatcgctac tgataatatg cgtaatgcca agctctttgg
    14701 cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg ctgtcagact
    14761 ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact aggtatacct
    14821 gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa tgctgattat
    14881 gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc tgcaggcatt
    14941 tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg agagcggtgt
    15001 accggaacaa ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa
    15061 tgcccgccgt ttacttccag aggctgattt ttgggtggcg attgaagctg gcattgatgg
    15121 cgacagcact ttcagttggg tggtgattga aaacgccagc cagcgcggcg aggcgcgttc
    15181 ggcaactttg ccgttacctg cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg
    15241 tcccgtgatg tcgcgttata ccggtattga tgagattggc cgtaaggaag gggcgatcgg
    15301 cgtgtttacc gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc
    15361 gctgagtccg tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc
    15421 cgcatccgac attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac
    15481 aaaatcaatc gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg
    15541 ctttcaggct gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta
    15601 actcacgctg actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca
    15661 acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt
    15721 ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca
    15781 tcgctgctga atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc
    15841 aaaatgactc ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga
    15901 tcagtaacga cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata
    15961 gcggtagtaa gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg
    16021 aatactctcg acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg
    16081 cacccgtcca ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg
    16141 ctgataaaca tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa
    16201 ttgcccaggg ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc
    16261 aggcataatc tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc
    16321 gctctcctga cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta
    16381 acgtttgaaa agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt
    16441 cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc
    16501 cagttgagcc tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc
    16561 ggtattatcg agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt
    16621 cagggtgctg tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat
    16681 gcgttgtgct gcaaccatcg ggtagaaacc acgctgttgc atgagttgat gcaaaatctc
    16741 tttcgcttca gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc
    16801 atctttctct tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc
    16861 gccggtgcca agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat
    16921 aatggcgtca tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca
    16981 ggcgacgata tcgcgcagct cctgaatctc atcttcatta agctgctgcg cctgggcaag
    17041 cgacgggatc atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac
    17101 cgccgccatt tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt
    17161 attagggttg ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat
    17221 ctgccctgcc agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg
    17281 ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc
    17341 acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc
    17401 ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc
    17461 gggcttttcc gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt
    17521 gacgaaacga gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt
    17581 agtgaccgtc accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta
    17641 gggataaagc ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg
    17701 attatcccag gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc
    17761 tcgcgccacg ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac
    17821 ttgtttggct ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcata
    17881 ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt
    17941 ttacttttac tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt
    18001 cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc
    18061 gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac
    18121 atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt
    18181 aagtccaccc tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt
    18241 ccgcagccag acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac
    18301 accgtgcgtg agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg
    18361 gatgaagtgt atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa
    18421 caaggccgtc tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg
    18481 gagcgtgcgg cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc
    18541 ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg
    18601 ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc
    18661 ctgcacgact tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac
    18721 gttgcaggct ggatcctcga actggaccgc ggtgaaggta ttccgtggga aggtaactac
    18781 tcctcctggc tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg
    18841 cgtcgtaagt cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag
    18901 tcgaaaggta aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa
    18961 cgtaacgaaa ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg
    19021 gaagtcagca acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc
    19081 tcgatcccga aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc
    19141 ctgttccgta tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa
    19201 acggtgaaac tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt
    19261 tgggaagaag tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc
    19321 cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa
    19381 ctttccggtg gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac
    19441 atgctgctgc tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa
    19501 aacgccctgc tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc
    19561 gaccgtatcg ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa
    19621 ggtaacttta ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag
    19681 ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc
    19741 gaacgcctta tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg
    19801 ataagacgcg gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac
    19861 accttttccg gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag
    19921 gttttttcaa accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa
    19981 aaaaactcat tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca
    20041 tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa
    20101 cccgcacgaa ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt
    20161 tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct
    20221 ccagcaggat caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct
    20281 cacgcccttc gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg
    20341 ccactttatt ggcgtatttc actgcaaaat caatgtattg tgcgtggccc aacgcgattt
    20401 tatcgtagtc ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat
    20461 cgcggccata ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg
    20521 tggatttacc gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca
    20581 cttcggtagg aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga
    20641 tactcataaa ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat
    20701 actgtggcgc atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag
    20761 ccataaactt tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc
    20821 cctcttcgtt gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc
    20881 agcgcagacg atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc
    20941 ggtcgcgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc
    21001 gctggataag gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa
    21061 tggttttctt ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt
    21121 gcgcgctggg gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac
    21181 cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt
    21241 aatcaaatga cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag
    21301 cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac
    21361 gttagccgcc ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc
    21421 ctggccactg ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag
    21481 atcctgtggc agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc
    21541 ggcgctggtt tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc
    21601 catttgcaga ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc
    21661 cagccccact gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat
    21721 ctccaccagc aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac
    21781 ttcatctccg cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc
    21841 ggtcatcgcg aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa
    21901 ggtgcgaaaa cgagagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc
    21961 cagcactttc gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc
    22021 cacaccgcgc gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac
    22081 gctaccaggc gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat
    22141 cagcttcggt tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa
    22201 catattgaga ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag
    22261 cgactcttcg ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg
    22321 cagcaacagc gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg
    22381 caccacgccg ccgcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag
    22441 ctcttcaagg ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata
    22501 gccgctgaga cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat
    22561 ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca
    22621 ttcattacaa acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt
    22681 gccgcccgca ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca
    22741 gaataccccc atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg
    22801 cttggcatgg taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc
    22861 atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag
    22921 agttttcgct ttgtactgtg cgtctacgat gtcgccgatc acattgccgg taaatttacc
    22981 gtccatgatc tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta
    23041 ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag
    23101 cttgagcacc agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag
    23161 aatattggcg tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc
    23221 gagttcgccg cgcatcgccc gttcggttac ctccgctacc atctcgcccg ttccggccag
    23281 cttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag
    23341 caaacccggc gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc
    23401 gtgggccagg cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc
    23461 ttccacgcac caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt
    23521 cagacgctgt ttatccagcc cacgaccata cagcagccag ccgctacgag ctgcgtggta
    23581 atccagtggc atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac
    23641 atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga
    23701 agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct
    23761 caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca
    23821 gtgattgtcc tgttctgtct tgccttgtta gtggcactga tgcagggagc gtcatggttt
    23881 agtcaaaacc accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg
    23941 cgtcaggtga cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc
    24001 attcaggcga tcctcgatca gttaacggat gaaagccgta tcctcgacgc gggtgtgtat
    24061 gacgaacaag gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg
    24121 gcgctcgacg gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg
    24181 ggtaaaaacg gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc
    24241 gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg
    24301 gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa
    24361 tcgcccttcc tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa
    24421 gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct
    24481 gactcttacg acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc
    24541 gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg
    24601 cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg
    24661 cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt
    24721 atggctggca agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg
    24781 aacgcgctcg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc
    24841 gaaggcgacc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac
    24901 cacggcacct tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat
    24961 aaaaagaaac tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc
    25021 gagctgctgc cggggctcac ccatgagcag gtttgcgagg ccataaccga ggcctttttc
    25081 gcccattatg gcgagcacgt ggaagcggaa atcatctccc cggacaaaac gccagacttg
    25141 ccaaacttcg ccgaaacctt tgcccgtcag agtagctgag agtggaactt cggtcaggct
    25201 ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga actgcatttc
    25261 gacgttgaaa aaggccatat cacccgcgcc caggtattta ccgacagcct caaccccgcg
    25321 ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa
    25381 caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct acgggagttg
    25441 tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaatatcca aggaaggaaa
    25501 tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca gccagcgtat
    25561 ttcctggcta gccgccgtgc gtatcaggca taaattatcg cttgctgaag taagtaaaat
    25621 gttaggtatc tcgattaatt ctctaaaaca gatcgaaaag acggaacggc ttagctcaaa
    25681 catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga tatgcccttc
    25741 ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa tgtattttat
    25801 tttctatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg atgaatagat
    25861 actaaaaagc cggagcagtc ccccactccg gcttaacacg acaatcgcct ttcagcgaaa
    25921 cacaattact ctttatcgcc cagcagaacg gattccagtg cgattttgat catgtcgttg
    25981 aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg gtcagacacg
    26041 gtgcagatgg tcagggcttt cgcgccaaac tctgcagcca cgccgtagat accagccgct
    26101 tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc gccgtccgga
    26161 gagtagaaca ggtcagcgga gaacaggtta cccacgcgag catcaacgcc cagtgcttta
    26221 gctgcatcta ctgcgttacg caccatgtca aagtcagcga tagcggcaaa gtcatggtct
    26281 ttaaaacgga tgcggttaac tttggaatcg gtgcaggccc ccataccgat aacgacgtcg
    26341 cgcagtttta cgtgcggcag aactgcgcca caggaaccaa cgcggataat tttcttcacg
    26401 ccgaaatcgg tgatcagttc tttggtgtag atggagcagg acgggatacc cataccgtga
    26461 cccattacgg aaattttgcg gcctttgtaa gtaccagtga agcccagcat accgcgaacg
    26521 ttgttcactt cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg
    26581 tcgcctggca tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta
    26641 gccattgttt tatccttttt gtgacataac aaaggcggag tcaacgctcc gcccaaatcc
    26701 atcagaacat ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct
    26761 ggccgatatc cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccat
    26821 ataccagtac cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcgcaac
    26881 cgtggtcagc ggtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca
    26941 gacggcggtc gaacagttcc agacccgcag cataaccggc gacgtcgcga cggtggcccc
    27001 aggaagagtc gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca
    27061 tctctttgat ggtggcgtca aacagcgcgt ccaagccagt cgctttcact tttttggtga
    27121 taccgcagtt ggcgtagatg tctgcaattt taccgacaga aaccacctgg ccgtgttttt
    27181 catcaaccag tttctgcagc acggtcggtg ctggcggctc aacagccagg tcgtgacggt
    27241 taccggtacg ctggaagtta ccagctttgt caccgataaa cggacgagcg ataacacgac
    27301 cgatattgta gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt
    27361 tatccagacc gaaagtttct tcatggcagg caatctggaa cacggagtca gcggaggtat
    27421 agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag ttgatccaga atgaccgtac
    27481 cggaagagtg gcagttaccg aggtaacccg gcagattagc gcgttcgacc agtttatcca
    27541 gcagctcttg cgggaagctg ttttcgtgat cggagaaata tccccactca aacagaaccg
    27601 ggacaccggc gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg
    27661 cccatgcgta cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg
    27721 tagaaccttc gtgtgctttc gccagcccca gacggatcag atttggcaga ttgagcggcc
    27781 ctttacgacc attatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt
    27841 cagccccgac gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca
    27901 gcaccataat aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga
    27961 tcagatcagt ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg
    28021 cgttatcggc aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt
    28081 cgtctttcgc gaggataacc gccagcggac gctgaccgtc tacctggtcg cccagacgcg
    28141 ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga tgcctgacga cgaccgccgc
    28201 ccattgcaac cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg
    28261 tatcagcata gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa
    28321 cgaagtcggt cgggcctttt tgtgccgcta ccatacgacc aaagacttct gccgctttac
    28381 cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc
    28441 cggagatcag catctccacg cacagcacca tcgtgacatc aaacagacgc gggttacgat
    28501 actcacccgt caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg
    28561 ccagtacctg attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca
    28621 cgccaacaat cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc
    28681 tacccacttt cacgtccatc accagcgcgt ccagaccttc cgccagtttc ttcgccagga
    28741 tcgaggcagt gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga
    28801 aacgtttatc agccggagcc agtgaactgg tctgaccgat aatcgccacg ccgacgtctt
    28861 taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca
    28921 gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc ggaatatagc
    28981 cgccgcaggc tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg acgccgccgg
    29041 tcgagtgttt atcaacaatc gggccatcca gatgcaggct tttccagtcg agaacggttc
    29101 ctgaatctcg catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga
    29161 aaatggtcat cgcgagggcg gcaatctgcc cttcggaaat agtgttgtcg cgaataccgt
    29221 tgataaagaa acgaatttct tcatcgctca gcgcatgacc atcacgtttt ttacgaataa
    29281 tttcttgtgc gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg
    29341 gatgcgtcta cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca
    29401 gcgcatcagg cgtaaagcat cttacttagt agctgctggc gctcttaccg tcgccgtggc
    29461 ccagcgcttt cagcaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat
    29521 cagcccagtc agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg
    29581 cagtacgcac gccgcccgcc ggtttgaaac caacggtttt ttctacgccc atatcacgga
    29641 tcacttccat catgatgcga gcgctttccg gcgtcgcgtt cacagccact ttaccggtag
    29701 aggttttgat gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg
    29761 cttcgtcttt cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc
    29821 aagcctcttt acaggctttc accaggtcaa aaccaacctg ctcgtcaccc gccatcagcg
    29881 cgcggtacgg gaataccacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt
    29941 ctgccagcgc gatttcgatg tcgtcgttac cgtgtgggaa gttggttacc gtagcgatac
    30001 ggatttccgg ggtgccctgc tctttcagag ttttgcgagc aatcgggata aaacgaggat
    30061 agatacagat agcggcggta ttgccgaccg gagttttggc ctgatgacac agagcaatta
    30121 ctttctcgtc ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca
    30181 ggctgcttgc tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc
    30241 ttgcgagttt gttagtattc taacatctac tccgcaacac acttcgatac acatcacaat
    30301 taaggaaatc tacttacaag tttgcatcac tgtaatgcaa tctggttcaa ataattcact
    30361 ttcaaatgaa tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact
    30421 tcttacaatg gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa
    30481 aatgcactgc aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag
    30541 aagcgccatt ttctggcact ggaagcagaa aacaatctgc cttacccaca gcttcctgcc
    30601 gaagcccgcc gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg tcatgcgccg
    30661 tacaaaccgc gctatgtctt acccgattac gctcgttttc tggcgaacgg ttccgaatgg
    30721 ctggagctgg aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac
    30781 caccacgtac cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg
    30841 caaccgtatg ttagaattct aacacaaaac gagatcgatg ttcgaataaa acgtttctgg
    30901 cgttacctcg acagaaccct gccagatgcc tttatgcacg ccaatatcgg cccgtctgat
    30961 tcgcccatta cccgcgcgat cttacgtgca gacgcgcagg tttcaccgaa cctgaccttt
    31021 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    31081 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    31141 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    31201 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    31261 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatacgcg gtgtgaattc
    31321 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    31381 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    31441 tgtgagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    31501 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    31561 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    31621 ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca cccatctgca aactgtcgcc
    31681 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    31741 aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    31801 gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    31861 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    31921 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    31981 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    32041 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    32101 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    32161 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtc tgcgagcaat gtgatacctg
    32221 cctgaagatg tgtccgcaac atgccacacc aatggcgcaa tccatgagcg tggacgaagt
    32281 gcttagccat gtgcgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    32341 agtcacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    32401 actgcgccat ctcacctgtc tggtggacag taacggtatg ttgagcgaaa ccggctggga
    32461 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcatggg gtagcgaatg
    32521 tcatcaacaa ttaaccggac gcgataatca acagattaag cgcagcatct atttgctggc
    32581 agagcgcggc aagctggcgg aactgcgttt gctgatgatc ccaggccagg tggattattt
    32641 gcaacacatc gaagaactgg cggcgtttat caagggactt ggagatgttc cggttcgcct
    32701 gaacgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    32761 ggaagacgtt gagccgttgg ctgatgcatt aaaggtgcgc ggggtgagcc ggttgatatt
    32821 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    32881 ctgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg
    32941 aacgccttat ccggcctacg gttcggcaca aacctgtagg cctgataaga cgcgttaagc
    33001 gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggca aactacccac
    33061 gaacgttaaa caccgcatac gtgttattaa gcaacacttc ggcaatctca tccgcgggtt
    33121 ccgggcgtaa ctcgcaaagc acggcaaaca cacgggcagc ctgttccggg cggttaggct
    33181 gcccctgaaa accgttgagc ggcatatccg gcgcatcggt ttccagcaac aacgatgcca
    33241 gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg atagtaccgc
    33301 ctacgccgat tttgtagccc agctgtacaa accgttcagc ctgttgctgg ctgccagaaa
    33361 atccgtggac aacaccagca cgcggcaaat cgtggcgttt aagatgcatc gccagtttgt
    33421 cgtgcgtgcg ccgtgaatgc aggatcaccg gcagatcgta gcgtttcgcc agtttcagtt
    33481 gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag agatccagac
    33541 cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc tgtagctgct
    33601 caagagacac atcgctatgt ttttccaaca taccaggatg caagcccaat gcggcataca
    33661 gcggttgata attttccgct aacgcctgca cgcgggcaaa attttccgcc tctgtcgccg
    33721 gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc tcttcatcgc
    33781 caataaaagg cgggaaatca aaatggcagt gagtgtcgat aaaacggcaa atcaagccag
    33841 atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagcg tcgctcactt cggcaaccag
    33901 cgtatcgttg gcgactggcg caggcggaat gacaatcgat tccggcgtca ccactggcac
    33961 cagatggcgg gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg cgaggaaata
    34021 acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc gactgccaag
    34081 cgcgatacta tgtaatggct tcggcggata aatttcggat atccgcagct tgcccggtgg
    34141 tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca ccagattgac
    34201 cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc actgcggcgt
    34261 gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc cctgccttgc
    34321 cgcctcttta accggaatcg catcactgat cccgccatcc aggtagttaa tgccttccag
    34381 cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca catccagcca
    34441 gttttgtttg gttggtaaaa agtaattcgg cgcgtagtca tcctgacgac aggcgcacat
    34501 ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca
//
LOCUS       DADLTI010000024        34522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLTI010000024 DADLTI010000000
VERSION     DADLTI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.024"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(52..258)
                     /locus_tag="JN755_001062"
                     /pseudo
     CDS             complement(52..258)
                     /locus_tag="JN755_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(259..345)
                     /locus_tag="JN755_001063"
                     /pseudo
     CDS             complement(259..345)
                     /locus_tag="JN755_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(439..1914)
                     /locus_tag="JN755_001064"
     CDS             complement(439..1914)
                     /locus_tag="JN755_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7379936.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2061..3345)
                     /locus_tag="JN755_001065"
                     /pseudo
     CDS             complement(2061..3345)
                     /locus_tag="JN755_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3458..5392)
                     /gene="yeaG"
                     /locus_tag="JN755_001066"
     CDS             complement(3458..5392)
                     /gene="yeaG"
                     /locus_tag="JN755_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7379937.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5828..6574
                     /locus_tag="JN755_001067"
     CDS             5828..6574
                     /locus_tag="JN755_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7379938.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6664..7425
                     /locus_tag="JN755_001068"
     CDS             6664..7425
                     /locus_tag="JN755_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7379939.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7476..8360)
                     /locus_tag="JN755_001069"
     CDS             complement(7476..8360)
                     /locus_tag="JN755_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7379940.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8444..9439)
                     /gene="gapA"
                     /locus_tag="JN755_001070"
     CDS             complement(8444..9439)
                     /gene="gapA"
                     /locus_tag="JN755_001070"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7379941.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9781..10194
                     /gene="msrB"
                     /locus_tag="JN755_001071"
     CDS             9781..10194
                     /gene="msrB"
                     /locus_tag="JN755_001071"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7379942.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10236..10508
                     /locus_tag="JN755_001072"
     CDS             10236..10508
                     /locus_tag="JN755_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7379943.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10878..11954
                     /locus_tag="JN755_001073"
     CDS             10878..11954
                     /locus_tag="JN755_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7379944.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11981..13360
                     /locus_tag="JN755_001074"
     CDS             11981..13360
                     /locus_tag="JN755_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379945.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13377..14422
                     /locus_tag="JN755_001075"
                     /pseudo
     CDS             13377..14422
                     /locus_tag="JN755_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14443..15279
                     /locus_tag="JN755_001076"
     CDS             14443..15279
                     /locus_tag="JN755_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7379946.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15284..16232
                     /locus_tag="JN755_001077"
                     /pseudo
     CDS             15284..16232
                     /locus_tag="JN755_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16242..17222
                     /gene="ydjG"
                     /locus_tag="JN755_001078"
     CDS             16242..17222
                     /gene="ydjG"
                     /locus_tag="JN755_001078"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7379947.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17359..18117
                     /locus_tag="JN755_001079"
     CDS             17359..18117
                     /locus_tag="JN755_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7379948.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18235..19593
                     /locus_tag="JN755_001080"
     CDS             18235..19593
                     /locus_tag="JN755_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7379949.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19686..20327)
                     /gene="pncA"
                     /locus_tag="JN755_001081"
     CDS             complement(19686..20327)
                     /gene="pncA"
                     /locus_tag="JN755_001081"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7379950.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20338..21354)
                     /gene="ansA"
                     /locus_tag="JN755_001082"
     CDS             complement(20338..21354)
                     /gene="ansA"
                     /locus_tag="JN755_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7379951.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21521..23377)
                     /gene="sppA"
                     /locus_tag="JN755_001083"
     CDS             complement(21521..23377)
                     /gene="sppA"
                     /locus_tag="JN755_001083"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7379952.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23538..24089
                     /locus_tag="JN755_001084"
     CDS             23538..24089
                     /locus_tag="JN755_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7379953.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24206..25249
                     /gene="selD"
                     /locus_tag="JN755_001085"
     CDS             24206..25249
                     /gene="selD"
                     /locus_tag="JN755_001085"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7379954.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25254..27215
                     /locus_tag="JN755_001086"
     CDS             25254..27215
                     /locus_tag="JN755_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7379955.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27342..28382
                     /locus_tag="JN755_001087"
     CDS             27342..28382
                     /locus_tag="JN755_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7379956.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28499..29842)
                     /gene="gdhA"
                     /locus_tag="JN755_001088"
     CDS             complement(28499..29842)
                     /gene="gdhA"
                     /locus_tag="JN755_001088"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7379957.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30078..30350
                     /locus_tag="JN755_001089"
     CDS             30078..30350
                     /locus_tag="JN755_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7379958.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30316..30723)
                     /gene="nudG"
                     /locus_tag="JN755_001090"
     CDS             complement(30316..30723)
                     /gene="nudG"
                     /locus_tag="JN755_001090"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7379959.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30810..31466
                     /locus_tag="JN755_001091"
     CDS             30810..31466
                     /locus_tag="JN755_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7379960.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ
                     "
     gene            complement(31475..32782)
                     /locus_tag="JN755_001092"
     CDS             complement(31475..32782)
                     /locus_tag="JN755_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7379961.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32849..33502)
                     /locus_tag="JN755_001093"
     CDS             complement(32849..33502)
                     /locus_tag="JN755_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7379962.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33502..34146)
                     /locus_tag="JN755_001094"
                     /pseudo
     CDS             complement(33502..34146)
                     /locus_tag="JN755_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(34103..34522)
                     /locus_tag="JN755_001095"
                     /pseudo
     CDS             complement(34103..34522)
                     /locus_tag="JN755_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062660.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT         8784 a         8106 c         8798 g         8834 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattga cagtacacct cagcgagagc
       61 gcgcatcaca tgcgcggcaa tactctgatt ctgatactta tcataaagaa aggaggagtc
      121 tgctttctgc acgatatagc gcagataggc actcattgct ctgtcgctgg cgacaagttc
      181 aaaaataagg aaggaagatg tcagcacaat gacacttgcg gcgataaaat gccggagcat
      241 tctatggtgc aatttcatga taaatggcac tccttcggtt agtgaaacga gaaaaccaga
      301 taaagttcga ttgcggcctg gcacaggggc gctaattata ctcatctctg cgccgctggg
      361 gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa aagccacgca aaaagcatgg
      421 ctctgaatat ttttgcactt aaatattatg aataatcact ttgttgcccc cctcgctttt
      481 cgcttcgcgt aaggctttat ctgcttccgt catgacaagc gagatattac gcgtgtcacc
      541 cgttgaaaaa aacgtgccaa tactgatggt catttgttca ggaacatcat atctgttact
      601 gaagccggtc aggagttcga catttttacg catacgttcc gcaatgattt ttgcctgggc
      661 actattgagt tcggtaaata gcaagccaaa cacctcaccc tccagtcgcg ctaaaatatc
      721 atcggggcgg atgctttgct ggataatatc gacaattgaa accagcactt tatcacccac
      781 acgatgtccc cattgggcat tgatgcgttt gaactgatta atatcaacca gcatgacaca
      841 gaagtcctta acaacaggtc gcgacaataa tgactctacg ctgttgaaaa agtagcgccg
      901 attataaata ttggtcagca catcatgaac tgccagtttg ctggagagtt gcagttcctg
      961 aaaaatgtta taaatcagaa aagacacaac aaacagttta ctggagactt caatcccgcg
     1021 actgatgtac cagatggtgt aattatactc atccagagtc agtagaagta agttgcagca
     1081 tagcgatgcc agacataata atgctattaa tggccatagt tcgctggcta atcgtgtacg
     1141 catgataata aagaacagta aaaatgccca cagacaaacc aatatttttg tatagttgat
     1201 tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg acatacaaag aatagtcagc
     1261 gttataactg ctcagattgt gtgccagaag gggaaaaact aaaaaaggaa tcagcgccag
     1321 caacagaatg ccagtttttt tcttattgtt gtcaaggatg ttgtctttac cataacaaaa
     1381 gagcgccaac gaggttaaac aaataaaact gagttggcgg aaaatataga aaatagaaac
     1441 atcattcgac tttgtctgga tcagtgtact gtcgctgata ggtttatgga taacaacaat
     1501 agtttcaact aaatagataa ggccacttaa aagtgcaaga cttaaaatga gtgttggtag
     1561 aatttgtttg ttagtgaaat atttcattaa catgaaaaga aaaatgattg cgttaaggaa
     1621 aaacaaagcc acaacgacca tcagataatt catttgtggg atgagtttta cattcgtctt
     1681 ttcgttatat aatgtggtga tgcaaaataa aattaacgtc agcaataaac tgaaaataaa
     1741 aaagctttga gcttttaatt tttgtgtgcc catagaaacc ataaaaagga gagcaatgtg
     1801 agtgctggat tttattgatg gcagagatat atagttttga ccaagatcaa ttttcgttaa
     1861 cgataaaaaa tatttaaaga ataaccccag ggaaatacgt gttgactgaa tcatttttcc
     1921 gaatgattgt tttctggaac taaattgtgt aaaggttatg ctgtcatatt taaagcgatt
     1981 gtaagctaat gtatgtaata aatgagataa tttacagtaa gctaaaaatc agccaggcga
     2041 taatgacccg gctgatagtt ttagtcttta gctgttgcat tttgtttatg aaacagttca
     2101 cggaacaccg gataaatatc atcctggtcg cggatgtgct gcatcgcaaa gttgtcgaaa
     2161 gtagattgca gatgctcata ttctcgcaca caatgtctga tgtgcacgac gggtaatttc
     2221 gatatagctg taataacgaa caacaggtaa taattttttc gccaggattt catggcaaag
     2281 cggagagtca tcggcccagt tatcgccgtc cgatgcttgt gcagcgtaaa tattccactg
     2341 tgccgggtta taacgctctt tcactacctc atccatcagt ttcagggcgc tggaaacaat
     2401 ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt tcgcctgggt
     2461 atgatggcgg atgtatacga cttccacgtt cttatacgtt ctgctgagga acagatacag
     2521 cagaatataa aaacgcttag ccatatcttt agtggattga tccattgaac cggaaacgtc
     2581 catcaggcaa aacatcactg cctggctgga gggatcgggc cgcttctcgt agttcttgta
     2641 acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt ttggcacgta attctgcaat
     2701 ttctttgcgc agacgttcct cttccagcag ctgcgcaggt tcactgttgc tgatgatggc
     2761 caaattctct tccagtgcat gaagttcccg ccgcttgcct gccgtcatgg ctgtgcgtcg
     2821 cgccagtgag ttctgcaagg aacgcacaac gctgatattg gctggaacgc cgttagcggt
     2881 ataacccgcc cgatgcgttt tatattcggt cagctggcgt tgttggtttt gtttcagatt
     2941 cggtaaggcc aaatcttcaa agagcagatc aagatactca tctttcgaaa tctgaaagac
     3001 aaattcatcc tgaccttcac catcctggct ggcctggccc tgaccactgc cggaacctcc
     3061 gccgccaccc tggggacgtt caattcggtc gttctgaacg aaatggtcat tgcccggatg
     3121 cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt ggttcgctaa tatcttccgt
     3181 gggaatggat acggattcgc tgctgtcgac gtcagtcacc gaacgcttat taatggcctc
     3241 ggagatcgac tgtttaattt gcgctttata acggcgtaaa aaacgctggc gattcaccat
     3301 gcttttgttt ttgccgttca gacgccggtc aataaaccag gtcatatgcc ccccgtacta
     3361 catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc aggcccacgg ttcgctatcg
     3421 ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta agacgattta cgtacgcgca
     3481 aataccattc gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc atcatacggt
     3541 cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta aacgagataa
     3601 ccggcaacag ctcctcggta ttggagaaca ttttcttctc gataaccgtg cgcagttttt
     3661 catagctggt ccagttcgga ttgcgtccgc tgttattcgc tctggcgcgc agtacgaagt
     3721 taacaatctc gttgcggaaa tcttttggat tactgatccc cgccggtttc tcgattttct
     3781 ccagctcggc gttaagagac tcgcggtcaa acagctgccc ggtatccgga tcgcgatact
     3841 cctgatcctg aatccagaaa tccgcgtagg taacataacg gtcgaaaatg ttttgcccat
     3901 attcggaata ggattcaagg taggccgtct ggatctcttt gccgataaac tcggcatatt
     3961 tcgggatcag ataacctttc aggaactcca gatagcgttc tgcctgctct tgcgggaact
     4021 gctcgcgctc gatctgctgt tccaggacgt agaacagatg gaccgggttt gccgccactt
     4081 ctacatgatc gaagttgaac acgcggggga ggatcttaaa cgcaaaacgc gtcgacagac
     4141 cgttcatccc ttcatcgaca ccggcgtagt cacgatattc ctgatacgac ttggctttcg
     4201 gatcggtgtc tttcagactt tcgccatcat aaacccgcat ctttgaataa atgctggagt
     4261 tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga cagtgtttcg agcgtgccag
     4321 gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa tttctcgtag attttgatct
     4381 cttcggaaat gcgcaagcaa tacggcacct tcacgatgta aacacggtcg aggaaggctt
     4441 cgttgttttt gttattacgg aaagtgaccc attcggactc gttcgagtgg gcgagaataa
     4501 tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt gtagttatct tcctgagtgg
     4561 cggttaacaa gggatgcagc acttttatcg gtgctttaaa catctcaacg aattccatga
     4621 tcccctgatt ggcgcggcac agcgtaccgg aatagccgta ggcgtccggg tcattctgcg
     4681 cgtagtgttc gagtttacga atatcgactt tcccaaccag cgcggagatg tcctggttgt
     4741 tctcatcacc gggttccgtt ttggcgatag caatttgttg cagaattgac ggccagacct
     4801 tcactacccg gaacttagtg atatcgccac caaattcatg caggcgtttt gccgcccatg
     4861 gcgacatgat ggtgccgaga taacggcgag gaatgccata ctctttttcc agaatctgag
     4921 catcttcctg cggattgaaa agacagaacg gatgatcgtt gaccgggcta cgctcaccgt
     4981 tggcgctcaa tacataaatc ggtacgagct gcattaatga tttcagtcgc tcagcaagcg
     5041 atgatttacc cccacccaca ggccccagca gatacaggat ttgtttcttc tcttccagcc
     5101 cctgagccgc gtgtttcaga taagagacaa tctgttcaat cgcgtcttcc atgccgtaaa
     5161 actcttcaaa tgccggataa cgtgcaatga cccggttaga aaagagtcga gaaagtctgg
     5221 gttcctgggc tgtatcgacc atgacaggct caccgatagc catcaatagc cgctcagccg
     5281 cgttggcata agcactgcga tcttgccgac aagtggtaag aaactcctgc agtgtgaact
     5341 cttcgtcctt ggcagcttca tatcgctggc gatagtgatc gaatatattc atggtatgcc
     5401 gtcctttcgt tttttagcac aggttaagag ccgttcatat gaatagtaga ggctcccgga
     5461 agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc attttatcga
     5521 cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg gcaaatggaa
     5581 aacttgcctg cacggctttc ttaatttcaa tggcatatca acaactcatc cataaatttt
     5641 gcataattaa tgtaaagacc aggctcgcca gtaacgctaa atccatttgg ctgtaagcgc
     5701 ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa aaatgaaaac gtaaaaaggt
     5761 tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt ttaaggaatg
     5821 attaattgtg accaaactca aacttctggc acttggagtg cttatcgcaa cgtctgcagg
     5881 cgtagcgcac gctgaaggta aattttccct gggcgcaggc gtaggtgtcg ttgagcaccc
     5941 atataaagat tacgataccg atgtttaccc agtaccggta atcaactatg aaggcgataa
     6001 cttctggttc cgtggcttag gtggtggtta ctacctgtgg aatgacgcaa cggataaact
     6061 ttcaattacc gcttactggt cgccgcttta cttcaaagcg aaagacagtg gcgatcacca
     6121 aatgcgtcac ctggatgacc gtaagagcac catgatggct ggtctgtctt atgctcactt
     6181 tacccagtac ggttacctgc gtaccaccct ggctggcgat accctggata acagcaacgg
     6241 cattgtctgg gatatggctt ggttgtatcg ttacaccaac ggtggcctga ccgtgactcc
     6301 gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa tactattatg gcgtatcgcg
     6361 caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca aatgacagct ggagccctta
     6421 ccttgagctg agcgccagct acaacttcct cggcgactgg agtgtttacg gtaccgcgcg
     6481 ctacacccgt ctgtctgatg aagttactga cagcccgatg gtggataaat cctggactgg
     6541 cctgatttct accgggatca cctacaaatt ctgataatgc atctttccag ggcattttgt
     6601 gcataaaaat agggcgtcac gccctgtttt gcattacggt ggtgcaatac ctgggaggaa
     6661 acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct cactgccagc catagggcag
     6721 ggaacatggt atatgggcga agatgccagt cagcgcaaaa cagaagttgc tgcactacgc
     6781 gcaggcattg aactcggttt aaccctcatt gataccgccg aaatgtatgc cgatggcggt
     6841 gccgaaaagg tggttgggga agcattaacc ggtctgcgag agaaggtctt tctcgtctct
     6901 aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga agccagttta
     6961 cgccgtctca atactgatta tctcgatctc tacttattac actggtctgg cagtttcgct
     7021 tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc agggaaaaat ccgtcgctgg
     7081 ggcgtttcta accttggtta tgccgatatg caggaactct ggcagctgcc ggggggaaat
     7141 cagtgtgcca ctaatcaggt gctttaccat ctcggttcac gagggattga gtacgatcta
     7201 ctcccctggt gccagcaaca gcagatgccg gtgatggctt acagtccatt agtccaggcc
     7261 gggcggttgc gcaatggact gttaaaaaac gcggtagtca acgaaattgc acatgtccaa
     7321 caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg aattagctat gctggataag
     7381 gcatatccgg caccaaaagg aaaaactgcg ctggatatgg tgtgatgttg atacgtgccg
     7441 gatgcatcgc acccggcaca caacattaac gcaaattaac gtttggcaac gcgaatggat
     7501 tgcgccagat gtgcaggttt ctctttggtg actttttgcg tttctgaagc gtaagccgtt
     7561 tctacacaaa caaaggtctt gtagccatcg tccggcatgt cgcccatgct aactgaaagc
     7621 gccggtcccg ggttccagcc gacaacgttc agatgatgct ggtggcctac ggcgataata
     7681 cgattcagcg cttcatcatt aatcacgctg caatcttgcg gattcagata cacgcgatcg
     7741 gtacggtcag ggaaggtctg aataccgtcg gtcagtacat cttctttcgc gtcattcact
     7801 ttatcaatga agcgatcgcc cagcccactg acgcttacct tagcgatatc acccacgtta
     7861 aagtaggtat gcagggcaga ggtggtttca aattcgccat gtgattcaag atcgatttca
     7921 caggttttac ccacgcggaa atgcgctaac agcgtaaagt cgtgcggcca gaattttttc
     7981 gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc catcagcatc ttcatgatgt
     8041 gatttcagcg tccacggcag gttgcgggca aaaccgtgcg caggcagacc ttgttgtgcc
     8101 gccggaccaa accacggcca gcaaaccggt acgctaccgc gaatagcgac gccatttttg
     8161 aacggtgtgt tgttgctcaa ccacagaact tcttcttcac ccgcaggttt ccacgagaga
     8221 aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg gatgatcgac cacaatgagg
     8281 tccagttcat ccagtttacg acgggagagg acaggggaga tttgttcgat gaccggaagg
     8341 gcaaaaattt tcttaatcat gacgcagtcc tttaacttca ttttatcagg taaaaaaaag
     8401 agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc aacttatttg gagatgtgag
     8461 cgatcaggtc cagaactttg ttggagtaac cggtttcgtt gtcgtaccaa gataccagtt
     8521 tcacgaagtt gtcgttcaga gcgataccag ctttagcatc gaacacggaa gtgcaaactt
     8581 cgccgttgaa atcggtagat actacgtcat cttcggtgta gcccagaacg cctttcattt
     8641 cgccttcagc agcagcttta acggcagctt tgatctgctc gtaagttgca gctttttcca
     8701 gacgaacggt caggtcaact acggatacgt tcggggtcgg aacgcggaac gccataccag
     8761 tcagtttgcc attcagttct ggcagtactt tacctacagc tttagcagca ccggtagagg
     8821 acgggatgat gttctgggaa gcgccgcggc cgccgcgcca gtctttgtga gacgggccat
     8881 caacggtttt ctgagtagcg gtagtagcgt gaacggtggt catcagacct tcgatgatgc
     8941 cgaagttatc gttgataact ttagccagcg gagccaggca gttggtggtg caggaagcgt
     9001 tggaaacgat gtcctggcca gcatatttgt cgaagttagc gcctttaacg aacatcggag
     9061 tgttgtcttt agacggacca gtcataacca ctttcttcgc accagcggtg atgtgtttac
     9121 gagcagtttc gtcagtcagg aacagaccag ttgcttcagc gacaacgtca acacccactt
     9181 cgtcccattt caggttagcc ggatcacgtt cagcggtaac acggattttt ttaccgttaa
     9241 cgatcagatg accgtctttc acttcaacgg taccgtcgaa acggccgtga gtggagtcat
     9301 atttcagcat gtatgccatg taatcagcgt ctaacaggtc gttgattgca acgatctcga
     9361 tgtcagaacg tttctgagca gcacggaaaa caatgcgacc gatacggcca aaaccgttga
     9421 tacctacttt gatagtcata tattccacca gctatttgtt agtgaataaa aggttgcctg
     9481 taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt gtcaatcatt gcgacagatc
     9541 aatcctgtgc ctaagcatta cgcgactgac tcgcctcact cttcctttgg gcttgagacc
     9601 acatggggtc ggcgccccga attttaaagg gcaattacga taaaaatgtg attttcatca
     9661 cgatttaggt gcagccattt cgtcgagatc aagtttagga caaaattttg ccagttggtg
     9721 ttaatgtttt gttagaatca gtcacgtaat gtgagcacga ttaaagtgag atgtgagtaa
     9781 atggcgaata aaccttcggc agaagaactg aaaaaaaatt tgtccgagat gcagttttac
     9841 gtgacgcaga atcatgggac agaaccgcca tttacgggtc gtttactgca taacaagcgt
     9901 gacggcgtat atcactgttt gatctgcgat gccccgctgt ttcattccga aaccaagtat
     9961 gattccggct gtggctggcc cagtttctac gaaccggtga gtgaagaatc cattcgttat
    10021 atcaaagact tgtcacatgg aatgcagcgc atagaaattc gttgcggtaa ctgtgatgcc
    10081 catctggggc atgtctttcc cgacgggccg caaccaacag gcgaacgtta ttgtgttaac
    10141 tctgcttctt tacgcttcac tgatggcgaa aacggcgaag aaatcaacgg ttgaaaaaac
    10201 gattcagcaa cttattccac aggagcggaa aaattatgaa tcttgatgac attatcaaca
    10261 gcatgacgcc tgaggtatac cagcgtttgt cgaccgccgt tgaactgggg aaatggcctg
    10321 atggcgttgc gttgaccgag gaacaaaaag agaattgcct gcaactggtg atgctgtggc
    10381 aagcccgcca taataccgaa gcacagcata tgacaattga cacaaatggt cagatggtca
    10441 tgaagagcaa acagcagtta aaagaagatt ttggtatctc agcaaagcct attgccatgt
    10501 ttaagtaagt ggttcatccc ctttttgccg cggctatgaa ggggatagcc aacaatgacc
    10561 ccacgactcg tctcaattat ctcgacccga cctggctaag tcccagtccg atctcttgca
    10621 gtcgcgttca cgcctgaatg acttctcctg aacttaagtc tcagacctat ttggccggta
    10681 atccctctcg aatttgctct ttttgcgtcc cgccttccac caacctgtaa cagacccagc
    10741 cattttctgc gtaattgtga tctaagttgt aaaatgcagg tcattatctg tgttgtgcag
    10801 atttaatgtt gacctcaaaa atcatgtggg taagtatgat ggccatctaa gcaaaacaaa
    10861 acacagagga tgacgcaatg aaagcactgg ctcggtttgg caaggccttt ggcggctaca
    10921 agatgattca tgtcccacaa cccatttgtg gcccggaaga cgtagtgatt gaaattaaag
    10981 ccgcggcaat ctgcggcgca gacatgaagc actacaatgt cgatagcggt tctgatgagt
    11041 ttaactctat ccgcggccat gagttcgcag gttgtattgc gcaggttggt gaaaaagtca
    11101 aagactggaa agtggggcaa cgcgtcgtgt cggataacag cggtcacgtt tgcggcgttt
    11161 gtccggcctg tgaacagggt gattttctgt gttgtacaga aaaagtaaac cttggtctgg
    11221 ataacaatac ctggggcggt ggtttttcca aatattgtct ggttcctggt gaaattctca
    11281 aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt tgattatgag gacgcagccg
    11341 tacttgaccc tatctgtaat gcctataaat ccatcgcgca gcaatcgaaa ttccttcctg
    11401 gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct gttttccgta caaatggcgc
    11461 ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca agaagatgtg gcggtccgct
    11521 tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa tggttctacc gaagatgtgg
    11581 tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg actggtgatt gaatgctccg
    11641 gtgccaatat cgcactgaaa caagccatcg aaatgctccg cccgaatggg gaagtggtac
    11701 gcgttggaat gggcttcaaa cctcttgatt tctcgattaa tgacattacc gcctggaata
    11761 aaagcatcat tgggcatatg gcctatgact ccacctcatg gcgtaacgct atccggctat
    11821 tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca tcgtatcggc ctgtcgcaat
    11881 ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat caaagtgatc atgacttacg
    11941 actttgatga ataactactc tcctgaagga acgttatcgc atggaacaga taacaaaacc
    12001 gcattgtggt gcacggctgg atcgcttacc ggattgccgc tggcattcgt caatgtttgc
    12061 catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc gttggtggct tgatcctcgc
    12121 gcagctgaaa gcgttgggct ggacagataa ttccaccaca gccacattct cagcaatcac
    12181 gaccgccgga atgtttctcg gtgcgctggt gggcggcatc attggcgaca aaaccggtcg
    12241 cagaaatgcg ttcatcctct atgaggccat tcatattgcc tcgatggttg tcggtgcttt
    12301 ctcaccgaat atggattttc ttattgcctg ccgttttgtg atggacgttg ggctgggagc
    12361 tttactggtt acactgtttg ctggtttcac cgaatatatg cccggtagaa atcgcggaac
    12421 gtggtccagt cgggtttcct ttattggcaa ctggtcatat tcgctctgct cactgattgc
    12481 gatgggactc acgccgctga ttagtgcaga gtggaactgg cgggtacaac tgcttattcc
    12541 tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc tactttcctg aatccccgcg
    12601 ctggctggaa tcgcgcggac ggtatcagga agccgagaaa gtcatgcgga gtatagaaga
    12661 aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg gttattgctg atgacggtaa
    12721 agcgccacaa gcggtgccgt attcagcctt actgacagga gtattactga aacgcgtgat
    12781 attaggctct tgtgtgctga ttaccatgaa cgttgtgcag tacacactaa ttaactggtt
    12841 gccaacaata ttcatgaccc aggggattaa tttaaaagac tcgattgttt taaataccat
    12901 gagtatgttt ggcgcgccat ttggtatttt tattgccatg ctggtgatgg ataaaattcc
    12961 gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg gtgctcggat atatctattc
    13021 actgcaaacc agtatgttgc tcataacgct gattggtttc ttcctgatta ctttcgtcta
    13081 tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa atctggccga cagaggccaa
    13141 actccgtggc tccggtctgg cgaatgcggt agggcgaatc agtggtattg ccgcacctta
    13201 tgccgttgca gtgctgctca gtagttatgg tgtaacggga gtctttattc ttctgggggc
    13261 tgtttcaatt attgtcgcaa ttgctatcgc caccattgga attgaaacca aaggtgtctc
    13321 cgttgaaagt ttaagtattg atgcagtagc caataaataa tacgggtgga ttcaaaatga
    13381 aaaattcaaa agcaatattg caggtgccgg gcacaatgaa aattatttca gcagaaatac
    13441 cagtgcctaa agaagatgaa gttttgatta aagtagaata tgtcggtatt tgtggttcag
    13501 atgtacatgg ttttgaatca ggcccgttta ttccgcctaa agacccaaat caagaaattg
    13561 gcctgggtca tgaatgcgcc gggacggttg tggctgtggg aagccgcgtg cgcaaattta
    13621 aaccggggga tcgggtaaat atcgaacctg gcgttccttg cggtcactgt cgttactgtc
    13681 tggaaggcaa atataacatc tgcccggacg ttgattttat ggcgacacaa cccaactacc
    13741 gcggcgcatt aacgcactat ctgtgtcatc cggagagctt tacttacaaa ctgcccgaca
    13801 atatggacac gatggaaggg gcgctggtgg agcctgccgc agtcgggatg catgccgcga
    13861 tgctggcaga tgttaaaccg ggtaagaaga taattattct gggagcaggt tgtattggtt
    13921 tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga aattgccgtc gttgatgtac
    13981 tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac agtggttatt aatggcgcaa
    14041 aagaggacac tattgcacgc tgtcagcaat ttaccgaaga catgggcgca gatattgttt
    14101 tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc tttaatctgg taatgcgcgg
    14161 cggtaaaatt atgattgttg gtactgtacc cggcgattcg gcaatcaatt tcctcaaaat
    14221 caatcgcgaa gtcactatcc agacggtatt ccgctatgcc aatcgttatc cggtcacgat
    14281 tgaagctatt tcttcagggc gattcgatgt gaaatcgatg gtgacgcata tttacgatta
    14341 tcgggatgta caacaggcat ttgaagagtc agttaacaac aagcgcgaca ttattaaagg
    14401 cgttattaaa attagcgatt aattaatacg ggagaacaga acatgctcgc agatatcaga
    14461 tattgggaaa acgatgccac aaataaacat tatgcaattg cccattttaa cgtatggaat
    14521 gcagaaatgt tgatgggcgt taaagacgca gccgaagaag cgaaatcccc ggttattatt
    14581 tcttttggta caggttttgt cggtaacacc tcatttgaag atttctctca catgatggta
    14641 tcaatggcac aaaaagcaac ggtgccggta ataactcatt gggatcatgg tcggagtatg
    14701 gagattattc ataacgcctg gactcatggt atgaattcat taatgcgtga tgcttctgca
    14761 tttgatttcg aagaaaatat tcgtttaacc aaagaggctg ttgatttctt tcatccgctg
    14821 ggtattccgg tagaggcgga attagggcat gtcggtaatg aaaccgttta tgaggaagcg
    14881 ctggcgggct atcactatac cgatcctgac caggcggctg aatttgttga acgtacgggc
    14941 tgtgactcat tggctgtcgc catcggcaac cagcatgggg tttatacgtc agagccgcaa
    15001 ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt ctgttccgct ggttttgcat
    15061 ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa tttcgttggg tattgcgaaa
    15121 atcaacattc atacggagct ctgtcaggcc gcaatggtcg cagtgaaaga aaaccaggat
    15181 cagcctttcc tgcatttaga acgtgaagta cgtaaagctg taaaagaacg ggcattggaa
    15241 aaaattgaac tgttcggttc tgatggcaaa gcggaataat gatatggata atctcgacgt
    15301 tatttgtata ggtgccgcta ttgttgatat tccattacaa ccggtcagta aaaatatctt
    15361 tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc accggggggc gatgcaataa
    15421 atgaagcaac aattatttct cgtctggggc atcgtaccgc attaatgagc cgcattggta
    15481 aagatgcctc agggcaattt attctcggcc attgtcgcaa agagaatatt gatattcaaa
    15541 gcctgaaaca ggacgtcagc atagatacat ctattaacgt tggactggtg acggaggatg
    15601 gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg gaaattaaat attgacgatg
    15661 ttgattttgc gcggtttcct caggcgaaat tattatcact ggccagtatt ttcaacagtc
    15721 ctctattgga tggtaaagcg ctaacagaaa tttttacgca agcgaaagcc cggcagatga
    15781 tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac gctggatgat atttgcgaag
    15841 cattgagtta tgtcgattat ctgtttccaa attttgccga ggcaaaatta ctcacaggga
    15901 aagagacact ggatgaaatt gctgactgct ttcttgcgtg cggcgtaaaa acggtggtga
    15961 ttaaaacggg taaagacggc tgctttatca agcgtggtga catgacgatg aaggtgccgg
    16021 cggtcgcagg aataaccgcc atcgacacca ttggcgcggg cgataacttt gcttcaggtt
    16081 ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg cgcacgcttt gccaatgcaa
    16141 cggcggctat ctcggttcta agcgtcggtg ccaccaccgg cgtaaaaaac agaaagctgg
    16201 tcgaacaatt gctggaagaa tacgaaggat aatgaaggca aatgaaaaag atacctttag
    16261 gcacaacgga tataacgctt tcgcgaatgg ggttggggac atgggccatt ggcggcggtc
    16321 ctgcatggaa tggcgatctc gatcggcaaa tatgtattga tacgattctt gaagcccatc
    16381 gttgtggcat taatctgatt gatactgcgc taggatataa ctttggcaat agtgaagtta
    16441 tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt tgtagtagaa accaaatgcg
    16501 gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt tggcgatcgg cagttgtata
    16561 aaaacctttc cccggaatct atccgcgaag aggtagaagc cagcttgcaa cgtctgggta
    16621 ttgattacat cgatatctat atgacgcact ggcagtcggt gccgccattt tttacgccga
    16681 tagctgaaac tgtcgcagtg cttaatgagt taaaggctga agggaaaatt cgcgctatag
    16741 gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct gcaatatggt gaactggata
    16801 ttattcaggc gaaatacagt attctcgacc gggcaatgga aaacgaactg ctgccgctat
    16861 gtcgtgataa tggcattgtg gttcaggttt attccccgct agagcaggga ttgttgaccg
    16921 gcaccatcac tcgtgattac gttccgggcg gcgctcgggc aaataaagtc tggttccagc
    16981 gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg gcagccactt tgtgctcgtt
    17041 atcagtgcac aattcccact ctggcactgg cgtggatatt aaaacagagt gatttaatct
    17101 ccattcttag tggggctact gcaccggaac aggtacgcga aaatgtcgcg gcactgaata
    17161 tcaacttatc ggatgcagac gcaacattga tgagggaaat ggcagaggcc ctggagcgtt
    17221 aaatactcaa cacgattcac ctctgtttct gtgaaaactg tgatgaaaat ctgtgattca
    17281 aataggttat tttgaaagta aacatcggtt atgcgataat cgcgctaatg tgatgtaaaa
    17341 agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc aggcaattaa gcaaatggtt
    17401 gccaacgata aaaaggtgac agtctcaaat ttgagtggga tttttcaggt aaccgaagaa
    17461 accattcgcc gcgatcttga gaagctggaa gatgaaggct ttttgaccag aacctatggt
    17521 ggtgctgttt taaatacagc ggtgttgacg gagaatatcc atttttataa gcgcgcttca
    17581 tcgttttatg aagagaagca gcttattgca cgcaaggcac taccctttat cgacaataaa
    17641 accaccatgg cagccgattc cagtagtacc gtaatggaat tgctcaaatt gttacaggac
    17701 cgtagtgacc tgacgttgct aaccaactcg gcggaagcga ttcatgttct ggctcagtca
    17761 gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa atacactttc cctgcaagga
    17821 agaattacta aagagatcat cagccgctac catgtcgata tcatggtaat gagttgtaaa
    17881 ggtcttgata ttaacagcgg cgcgctggac tctaatgaag cggaagctga aatcaaaaag
    17941 acaatgatcc gtcaggcgac agaagttgcg ttattggttg atcactctaa gtttgatcgt
    18001 aaagcttttg tccagttagc tgactttagt catattaatt atataataac tgataaatca
    18061 ccgggtgcag agtggatagc attttgcaaa gacaataata tccagctggt gtggtaattt
    18121 aagcacacgt tgtcaatatt atttgttcct gcgcctggaa ataaaatgat gataaagatg
    18181 cggcaagtga atttgccgca attgctacct tttatagcaa atccgaggca cccaatggaa
    18241 caatatgatc aaattggcgc aagactggac cgcttgcctt tggcccggtt tcattatcgt
    18301 atatttggta taataagctt tagtctgtta ttaacggggt ttttgagtta ctcaggtaat
    18361 gtcgtcttag caaagctggt aagcaatgga tggtcaaata atttcctcaa tgccgccttt
    18421 acctcggcat taatgtttgg ttatttcatc ggctcactta ctggtgggtt tattggtgac
    18481 tactttgggc ggcgcagggc gtttcgcata aatcttctca tcgtcggtat tgctgcaaca
    18541 ggggccgctt ttgtccctga tatgtactgg ctcattttct tgcgcttcct gatgggaaca
    18601 ggaatggggg cgctgattat ggttggctat gcctcattta cggagtttat ccccgcgacg
    18661 gtgcgtggaa aatggtccgc gcggctctca tttgttggta actggtcgcc catgctgtct
    18721 gcggcgatag gcgtggtggt tatcgctttt tttagttggc gaataatgtt tcttctgggt
    18781 ggtattggca tactgttagc ctggtttctc tcaggtaaat actttatcga gtcgccacga
    18841 tggctggcag ggaaagggca aatcgccggt gcagaaagcc aacttcgtga agtagagcag
    18901 caaattgaaa gggagaagag tattcgttta ccccagctta ctttgaacca gagcaacagc
    18961 aaggttaagg taatcaaggg taccttcagg ctcctgttta aaggggaaat gttacgacgt
    19021 acattagttg cgataactgt tttaattgca atgaacattt cgctttatac cattaccgta
    19081 tggataccga ccatatttgt taactccggc attgatgtcg ataaatcaat attaatgacc
    19141 gctgttatta tgattggtgc tccggtagga atatttattg cggcattaat tattgatcat
    19201 tttcctcgtc gattatttgg ctccgcctta cttattatta ttgccgtgtt aggctatatc
    19261 tattcaagtc agactacaga gtgggcgatt ttaatctatg gtctggtgat gatcttcttt
    19321 ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc cggagctttg gccaacgcat
    19381 ttacgcctgc gcggttcggg tttcgttaat gccgtcggac ggatcgtcgc agttttcacg
    19441 ccctatggcg ttgcggcatt attaacacat tatgggtcga tcacggtgtt tatggtgctt
    19501 ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt ttggcatcga aacgcggaag
    19561 gtgtcgctgg aagagatttc tgaggtgaat taaatctttc gttgatggca tcaattgcca
    19621 gatgtaccac tgacgtgttt tatttggcct acaatgccag tacagaattg taggccagcg
    19681 taaaattacc cctgcgtctc ttcccagtct gccagcgtat atagcgttgc cccagctgct
    19741 gacatctcca taaacgcgtg tgcactgtcc tggggctgga tattcacgcc acgacagcca
    19801 tcggtaatca cgtttacctt ataacctaac tgtaacgcgt ccagcacggt aaacttcacg
    19861 caatagtcag tagccaggcc cataacgatc aattcatcga tttcatgatc gcgcaaccag
    19921 tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa aggcactgta actgtcaact
    19981 aaaggatttt cgcctttatg gaacaccgct gcgatctctt tttgattcag taacggatgt
    20041 aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc agaaagtttg ccgcaggccg
    20101 tcgagttggc ctggcgtata aggctctacg ccgtgctggc tggcaaagct gccgtgattc
    20161 gccggatgcc agtcctggct ggcgataacc gcttctccgc gcgactggca ccagtcaata
    20221 aggcggttag caacctccac cgtactgtca ccttccggta cggcgagcgc gccaccagca
    20281 cagaaatcat tttgtaaatc gaccagtaac agggcgcgat ggggcattgc agtctcctta
    20341 atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg gccttgcgaa tggtttcagt
    20401 atccagttcc tggctcagca ggtaatgcag tttggttagc gtggcttcta cagtcatatc
    20461 tgcaccgcca ataacgccgg catgggcgag gacgttaccg gtggcataac cactcatatt
    20521 cactttaccg gacatacatt gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc
    20581 ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta cccacgccat aggagcgcag
    20641 aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg acgtcagcag aaatccctgg
    20701 ataaatcgtc actacgccaa ttggttgtgg cgtgattgga tgaacgatta attccccttc
    20761 accgtgcggg gcgggtggcg tattcaaacg acgaatatgg ataccagctt ccagtaacgg
    20821 aggaaggttt ggagaggcaa acgcatcaaa accatcggca tgggctttgg tagtgcggtt
    20881 gccgcgatac aatcggttat tgaaaaagag cgttacttcg ttgatcggat aattcgccgc
    20941 aacgtacaac gcattcagca gattaatttg tccgtcagag cgtaactcag ccagcgggat
    21001 ttgtgaccct gtcacaatga ccggtttacc gagattctcg agcatgaacg acagcgcaga
    21061 ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca aaaccatcat agtcgtcata
    21121 gtgcgcttta atatcttcag caatatgctg ccagtcttcc ggcgtcatat cagaagaatc
    21181 catcaacggc gtatattcat gaatggtgaa atctggcatc tccaggcgat ggaattccgg
    21241 catcagcgcc agttggcgtt gtagatgacc tgacaccgga atataaccct gctcggaacg
    21301 ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt
    21361 gatatgaatg atatcgaaag agcaaaagta tatacgtgag gcaggaggag cgcacctgat
    21421 actatgcgga taaggaaata tggggcgaga cagcgggaag aggattcgtc gaggtaaaaa
    21481 ataatgtggc cgacgatctg tcggccactc agtacaagac ttaacgcacg ttggcgcagg
    21541 tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc ggccagcttg tcgctttcac
    21601 ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg
    21661 gcaacattgc ccggacagaa ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt
    21721 catcaacgta gtattccaga tgccactgtt tcacttttgc gagctctgct gctttggcaa
    21781 ccgcatcatc gaaatccccg agactatcga ccagcccgtt agcttttgca tcctgaccgg
    21841 tccagacgtg gccctgggcg attttatcaa tttgctccgg cgtcgaatga cgcgcatcag
    21901 caaccagcgt gataaagcgt ttatagccat tctcaatgct taattgcatc atctgctgcg
    21961 cttccggcgg cagtgccctg gtgatagaaa catccgccag cggtgaagtt gagacaccat
    22021 cagtatgaac accaatcgaa tccagactat tttctacggt ggtgatcacg ccgaagatac
    22081 cgatagaacc ggtcagggtg ctggggttag ccacaatgta attagctggc gtggaaatcc
    22141 agtaaccacc agatgccgcc atgccgccca tcgatacaac cacagactta cccgctgccc
    22201 gggctgctgc cagttcagcg cgaatcactt cagacgcggt aacgctgccg cctgggctat
    22261 taacacgcag gacaatcgct ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg
    22321 cagtggtatc accgccaaca ttcccctgag tttcctcgcc atccataatt gcgccattag
    22381 cgaagacgac accgatgctg tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat
    22441 aactgatggc gcgataattt ttatcagtct tactccagcc gaactctttg gtcagagctt
    22501 tttcgatttc ggcactcgat gccagtgcat cgaccagctt gttttccagt gcatatttcg
    22561 cggtatcgcc accggttttg gttaaaccct caagcaaccc ttgcgcgcca gggaatacct
    22621 gctgagcagg gatctgccgg ttagcggcaa cagtattcag atagttttgc cacagctcac
    22681 caatccagcg gctgtcagct tcgcgggctg ccggtgacat atcatcacga ataaacggtt
    22741 caacggcaga tttatacgta cccacgcgga acacatgggt ggaaactttc agcttatcca
    22801 gcaacgattt gtagtacaga ccgttggtag caaaaccgtg cagatcaacc acgccttgcg
    22861 gtgacagcca aattttattg gcgaaactgg cgagataata ttgcccctgg ctgtagttct
    22921 cgccaacggc ataaaccggt ttcccgctgt cacgaaactc tttcagggct ttgccgatgt
    22981 actgcataga cggctggtcg ccgcctgcga agtttttcag atccatcacg ataccggtga
    23041 tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat atcaaacagt gagttttcct
    23101 gcagacgatc ggaactggca ccaagcagct ggcggcttaa tttactaaac cgctgagaac
    23161 tgtctggttt atcgacgatc acaccagaaa tgtccagcag cagtgcgcca cgactggccg
    23221 tttctttcga atcaccacca ctgacctgca tccagatccc cacaccaacc agtacgagga
    23281 aaataaagaa caggttaagt accatttcac ggacgaaatt cagcagacgc cacgtccatt
    23341 taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac
    23401 acctgtcaca ggcgcaatag atgtggttat cgtaatgacc caaccggcac ttgtcagcag
    23461 gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt aattttgtga ataaatatca
    23521 cgacaggagt taatcaaatg gatgcactcg aactattgat caatcgccgt agcgcctccc
    23581 gcttggctga acccgcgcca acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc
    23641 gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt tgtgattgaa ggggaagggt
    23701 gcgagcgttt cagcgccgta ctggaacagg gggcgattgc tgccggtagt gatgataaag
    23761 ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct catcatcacg gtggtggcga
    23821 aatgcaaaga aaatcataaa gtcccgcgat gggaacagga aatgtctgcc ggatgcgcgg
    23881 tcatggcgat gcaaatggca gccgttgccc agggatttgg cggcatctgg cgcagtggcg
    23941 cattaactga aagtccggta gtgcgtgaag cattcggttg ccgtgagcag gataaaattg
    24001 tcggttttct ctacctcggc acgccgcagt tgaaagcatc tacgtcgatt aacgtcccgg
    24061 acccgacgcc gtttgtaact tatttctgat aattgtcgcg aaactgtctg gattgtgagc
    24121 aaaggcgcag gaattcagac actctcactt atcacttcac ggaatgaggg ttaccatacc
    24181 cggattgcat tgacaggaga tgtccatgag cgagaactcg attcgtttga cccaatacag
    24241 ccacggagct ggttgcggct gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag
    24301 tgagcaggcg aagtttgttg atccgaattt gcttgtgggt aatgaaaccc gcgacgatgc
    24361 ggcggtgtac gatctgggca atggcaccag cgttatcagt accaccgact tctttatgcc
    24421 gatcgttgat aatcctttcg attttggccg cattgcggcg actaacgcca tcagcgatat
    24481 cttcgcgatg gggggcaaac cgattatggc gattgcgatc ctcggctggc cgattaacaa
    24541 actttccccg gaaattgccc gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc
    24601 gggtattgcg ctggctggcg gtcactccat cgatgcgccg gagccgattt ttggtctggc
    24661 ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac agtaccgcac aagccggatg
    24721 caaactgttc ctgacgaaac cgctggggat cggcgttctt accacggctg agaaaaaatc
    24781 actgttgaaa ccagaacatc agggactggc gacggaagtg atgtgccgga tgaacatcgc
    24841 aggcgcgtct tttgccaaca tcgaaggcgt aaaagcgatg accgacgtta cgggctttgg
    24901 tctgctgggc cacttgagcg aaatgtgtca gggggctggt gtgcaggcac gcgtcgacta
    24961 tgaagcgatc ccgaaactcc ccggtgttga agagtacatt aagttgggcg cagtacctgg
    25021 cggcactgaa cgtaactttg ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt
    25081 gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga
    25141 agcagaaaat gaggttaaaa ctacagccgc cgagtttggc attgaactga cggcgattgg
    25201 cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag atccgttaat tcaatgcggt
    25261 tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc
    25321 accggaaagg cgatggcttt atcgagtgcg gtaatggtca ggtggtgact tggtgtatcg
    25381 gtcacctgct tgagcaggcg cagccagacg cctacgacag ccgctatgcg cgctggaatc
    25441 ttgcggattt gccgattgtc ccggaaaagt ggcaattaca gccacgaccc tccgtgacca
    25501 aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag cgaaatcgtt cacgccgggg
    25561 acccggatcg tgaagggcaa ttgctggtgg atgaagtgct ggactatctg caactggcac
    25621 cggaaaagcg ccagcaggtg cagcgttgct tgataaacga cctgaacccg caggcggttg
    25681 agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt gccattgtgc gtttctgcgc
    25741 tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat gacccgtgcg tataccattc
    25801 tcggtcgcaa tgccggttat cagggcgtac tttccgtggg acgcgtgcag acacccgtgc
    25861 tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg
    25921 aagtcaaagc acatatcgtg acacctgccg atgagcggtt taccgctatc tggcaaccga
    25981 gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt gttacatcgt ccactggcgg
    26041 agcatgtggt taaccgcatt agtggtcaac cggctattgt caccagctat aacgataaac
    26101 gggaatcaga atccgcgccg ctgccgtttt cgctttcggc gttgcagatt gaagcggcaa
    26161 aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg ccagaagctg tacgaaacgc
    26221 acaagctaat cacttatccg cgttccgatt gtcgctattt gccagaagaa cattttgccg
    26281 gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc ggatctgttg ccgcagccag
    26341 tggtagatcc agatatacgc aaccgctgtt gggatgacaa aaaggtcgat gcgcaccacg
    26401 ccatcatccc gaccgcacgg agttctgcga tcaacctgac ggagaacgaa gcgaaggtct
    26461 ataacctgat tgcccgtcaa tacctgatgc agttctgccc ggatgcggtg ttccgcaagt
    26521 gtgttatcga actggacatt gccaaaggca aatttgtcgc taaagcccgt tttcttgctg
    26581 aagcaggctg gcgcacgctg ttaggcagca aagagcgcga tgaagaaaac gacggcacgc
    26641 cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc
    26701 ggcaaaccca gccgccgcgc cattttaccg atgcaacact gctttcggcg atgaccggga
    26761 tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct tcgtgcgacc gatggtctgg
    26821 ggacagaagc aacccgtgcc gggattattg aactgctgtt caagcgtggt tttctgacca
    26881 aaaaagggcg ctatatccac tccaccgacg ccggaaaagc gttattccat tcgctgccag
    26941 aaatggcgac gcgaccggac atgaccgcgc actgggaatc ggtgctgacg caaatcagcg
    27001 aaaagcagtg tcgctatcag gactttatgc agccgctggt ggggacgcta tatcagctta
    27061 ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg cattgtggct ccgggcagtg
    27121 gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg tagtgcgaaa aaaagtccgc
    27181 cagcagatga agccggaagc ggggcgatag cgtaagcgag cgaatctttc gtgctattcg
    27241 agtcatattc tgaaatatcc agcggatcaa gaaaattcgt tggatatttt tttgcatgga
    27301 taaaattatc gcctctaaag tatgtaataa cagggaatgt gatgaaaaaa gttttattac
    27361 aaaaccatcc tgggagcgag aagtattctt ttaatggctg gggaatattt aatagtaatt
    27421 ttgaacgggt gattaaagaa aataaggcca tgctgctttg taagtggggg ttttatttaa
    27481 catgtgttgt cgctgtaatg tttgtattcg cagcgataac atccaacggt ttgaatgaaa
    27541 gaggcctgat taccgcggga tgctcttttc tttatctatt aattatgatg gggcttattg
    27601 ttcgggccgg ttttaaagca aaaaaagaac aactacatta ttatcaggct aaaggtattg
    27661 agccgctcag tatcgagaag ttacaggcgc tacaattgat cgcaccttat cgattctatc
    27721 ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa gcctgaacct ggcaaagata
    27781 ccttccaata tcatgtgctt ccttttgact cgatcgatat catcagtaaa agacgcgagt
    27841 ctttagagaa tcaattgggt atcgaagata gcgaaagtta ttgtgcctta atggagcatt
    27901 ttctttctgg cgaccatgga gccaatacct ttaaagcaaa catggaggaa gccccagagc
    27961 aggttatcgc cgtgttgaat aaatttgctg tttttccctc agactatatc tctgattgcg
    28021 ctaataatcg ctccggtaaa tcctcggcga agctaatatg ggcggcggaa ttatcatgga
    28081 tgatctcgat atcaagcaca gcttttcaaa acgggacaat tgaagaagaa ctggcctggc
    28141 attatataat gcttgcttct cgaaaggcgc acgagttgtt cgaaagcgaa gaagattatc
    28201 aaaaaaatag tctaatggga tttctttact ggcatatctg ctgctatcgc agaaagttaa
    28261 cggatgctga acttgaagcg tgttatcgtt acgacaagca gttttgggag cactacagta
    28321 agaaatgccg ttggcccata agaaatgttc cctggggagc atcatccgtt aaatactcat
    28381 aaacgcctga aattttgccg ggggcgctgc gcttgcccgg cctacattag agcgtaactg
    28441 caatgaattg tgcccatctg taggcctgat aagcgtagcg ccatcaggca tttacaactt
    28501 aaatcacacc ctgcgccagc atcgcatcgg caaccttcac aaaaccggca atgttcgcgc
    28561 cctgcacgta gttggtttgc tcaccttcac caccatgctc aacacaggca tggtggatat
    28621 ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc tttccagccc aggcgcgcag
    28681 cgttttgtgc catttccagg cccgacgttg ctacaccgcc agcattagcc gctttacccg
    28741 gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc ttcgatggtg gtcggcatat
    28801 ttgccccttc agcgacggct ttaacgccat tggcgataag ctgatgcgcg gtgtcaacat
    28861 ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac cggtacagac cacggctgtt
    28921 ggccttcgag atagaccaga ccaaattctt tggcgtaatc tgccactcga ccatcgcggc
    28981 tggctttgat ttcgataaga cgtgccagtt tctctttcgt gaatccgctt tcatcaacta
    29041 cagtgccgct ggagtctgac gcagtgatca cacgagcacc aaattccatc gctttttcga
    29101 tagcgtactg ggcgacgttg ccggagccag aaacggaaac gcgcatccct tcaaaaccca
    29161 taccgtggcg ttttaacatt gcttctgtga aataaaccag accgtagccg gtagcttccg
    29221 ggcgaataag actaccgcca aatgaaaggc ccttaccggt gaagacgcag gcggtattgt
    29281 tggagagctt tttcatcatc cccgccataa agccgacttc acgaccacca acgccgatat
    29341 cacctgccgg aacgtcggta tccgcgccca ggtggcgata cagttcagtc atcagcgcct
    29401 ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg atcgaaatcg ctgccgcctt
    29461 taccaccgcc catcggcagg gtagtcaggg catttttgaa ggtttgttca aagccgagga
    29521 atttgagaat ggaaaggtta actgacggat ggaagcgcat accgcctttg tacgggccga
    29581 tggcagagct gaactgcaca cgccatgcac ggttgacctg tatctggttg cgatcatcaa
    29641 cccataccac gcgaaactgg atcacgcgct ccggttcaac cagacgctcc agtaatgaca
    29701 tctggcgata ttttggattt tgttcaagaa aaggccagag tgtggtcatt acttcacgaa
    29761 cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg gacatggttg aggaatgact
    29821 ccagagaata tgtctgatcc atagatataa aaaccttata tattaatacg attgtgctta
    29881 tgttgtttat gtcatgtgct tttgcagttt tcgactatac catcaagaat gtgacggtaa
    29941 gcaagaaaaa aatgacccag gaaagcaaat taataacgag agtaatctca taaccaaatg
    30001 cgataactca gacgctaaag ttttgccacc cggctaccgt tatcagtagc agagacaata
    30061 acgttaagga gttccctgtg agtcgagcat tgttcgccgt agtattagca tttccattaa
    30121 ttgcactggc taacccgcat tatcgtccgg acgtggaggt aaacgtaccg ccagaagtgt
    30181 tcagttcagg cgggcagtca gcgcagccct gtactcagtg ctgcgtttat caggatcaaa
    30241 attattctga aggggcggta attaaagccg aaggaatact gctgcaatgc cagcgtgatg
    30301 acaaaaccct tagcactaat ccgctgctct ggcggcgcgt aaagccataa acgcctctaa
    30361 taatggaatg tcagcagggg ccagcggata ttgcagcgcc tcttcaggtg agcaccagac
    30421 cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt gccaggcatg
    30481 aagatggata atccggcccg aaacttctcg ctgatggctg gcaacatact cacccacagt
    30541 tgcttcgatg tccaattctt cgtttaactc acgcaccagc gcctgctgct ggctttcatc
    30601 cagctcgact ttacctccgg caaactccca taatcccgcc tgatcgctct gggcggggcg
    30661 ttgcgcgagt aaaattttgc catcacgttc aatgatggcg gcaacaactt caatcatttt
    30721 cataaattac acacgggcag ataagcgaaa aatgccataa tatccgcact gttgctcaat
    30781 cagaaaggct ttatatttaa ggagtgcctg tgctagaccg ccatcttcat ccccggatta
    30841 aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc gggcattacg ccagatggtt
    30901 taacattagt gggatttgcc atcggcgtgc tggctctgcc gtttctggcg ctgggctggt
    30961 atctggcggc gctagtcgtc attttgttga acaggctgct tgatggtctg gacggtgcgc
    31021 tggcgcggcg cagagggctt accgacgcgg gcggctttct tgatatttct ctcgattttc
    31081 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
    31141 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
    31201 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
    31261 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
    31321 ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc gtggtttgcg tggtttgcgt
    31381 ggatcttcgg tgcgttgtgc tggatgacga cctttacgcg ggtgtggagt gggtatctga
    31441 cgctgaagtc gctccagcgt cagtaattgc aatgttattt gctactgccc gggccgcgtt
    31501 caccggttgc aaccggattt tttggatcgc tgctccattc gtaccagccg ccgtcataca
    31561 cggaaacatt cttccaaccc atggcgcgtg cgtacataaa ggtttcggac gcgcgccagc
    31621 cggtgccgca gtagaatgaa acttgctgat ctggtttgat attccatgct ttccacatag
    31681 cggtaatatc atcggcgcta cgcatggtgc catccgggtt atggaaatct tccatatgcg
    31741 tcgagtcgct accagcgtgt ccccaacgtg ctccggctat ttcacctttt ggtttaatat
    31801 agctgtaacc gctggtcgta ccgataaatt ctggccacga acgaatgctc accagcgatg
    31861 catcctggcg atgcagcagt ccacgggcct gttccatatc gagcatcaac tgcggttgtg
    31921 ccgggatctt cacgccgaaa tctggttccg ctttcacttt cggtggcgtt cctcgctcaa
    31981 caggcagtcc cgcgtcggac caggtttgcc agccgccatc cagcagacgc acatctttca
    32041 cgcctgcgta gagcataatt tgcgccacac gcgctccagc gtatacgtca cgcccgtaca
    32101 gaatgaccgt ggtgtcatgg cgaatgccgt gttttgccag catcgctttt agttgttcat
    32161 cagaaacttt gttccacagc ggttcgcttt ccacttcgtt ggtatcgatg tagtcagcgc
    32221 caggaatatg gctgataagg taaagcttag gagcgcccca ggccgcttca atgactttcc
    32281 agccaccgac aggtttcgcc gtaacctctt taccttgttg caggtcatgc agccattgcg
    32341 gataaaccag ctgctcaaag tgcggcagtt tttgtagacg ggaaggttcg cttagcgcgt
    32401 cactcagaat ggagatatgc gtaaaacctg ctttttgcag tcgcgttttg acggcgtcga
    32461 catctttgcc attaccgtac agcgccaccg gggcatcggc tttcaggtta tgttgcttga
    32521 tccacgcgtt gagctgttcg gtgctcattt tgtcgagcca gctagcagag aggtttaagg
    32581 cggcaggttc atgaccagaa gggccattta aggtttgtgg ccagccgtta taaaacgcgc
    32641 tggggcgagt atctatcgct ttgccatttt gttgctgaag ttggtcaagt gtaagaggct
    32701 tcgccagttc agcggcccac gaagaagcgc aagccagccc taaagccatt gccagcgcgg
    32761 tcatttgaga aacacgtttc atcgaaaatc ccgacattat agttttgagt gcgccattct
    32821 gaactgacgc attgagaaaa actttgcgtt atcgcagttt gttgtaattt tctgaccact
    32881 gcgccatatc cagaacagaa ctatcagcag gaacatcctg gagatcgtgc gttacctgaa
    32941 cgacggggat cgccagggcg cgaacttcgc tgaacaccca ctggcgaaaa ttatcgcgca
    33001 gagccacatc aagacggctg aatggctcat ccaggagtaa cgcttttggc tgggcgagaa
    33061 gggcgcgtag tagagcaacg cgcgctcgct gaccgccaga caaagtggca ggatcctgat
    33121 ggaaagctcc gtcaaggcct gaacgctcaa gtgcatcatt cacggcatta cgtcgggcat
    33181 tccccttaag tgtcgccggt agcgccagca gtaaattttg cccgacgctg aactggtcaa
    33241 ataacagtgc atcctgaaaa agaatgccaa tctgacgctg tgcggtgggt agcatgtcaa
    33301 tccgttgctc attgagccat agctcacctg tacaagaaaa ctgtccggcc agtgcaccaa
    33361 tcatccatga aaacagagtg gattttccac agccagatgg ccccattaac gtgacgatgt
    33421 cacctttatc aaccgtaaag ttaacgtttg tcagcaagcg gctttctggt aaacgtagcg
    33481 aaacattttt cacgcagagc attagcggat tccttgtctg acataaccta cccattttgc
    33541 gactaacgcg gtcagggcaa aaataataag cggtaacaac agttgccata aagcctgggc
    33601 ggcgagaata ccgttgctgc cgccgctgct taatgccacc gcctcagtgg tgagcgtcgg
    33661 aaaacgcccc gcgcccagcc acagcgtcgg catatactgc gcaatactga ctgaaaatcc
    33721 caccgcgaag gcaatcagca cagggcgcaa catgagtggg catttcacgt aaaagaagat
    33781 tttggcccgc gaccagccca gtgtttgcgc aatcaaaatt aaccgtgaat caatgcgctg
    33841 ccaggcgggt tgcaggataa acagcatcca cggcattacc cacagcagat gcccccagac
    33901 caccgccgtc cagcttccat ccaggttcag ccatagcgcc agcgtgtact agcccgccac
    33961 cagtggcaga gcgggcaata gaatgggcaa ccatagccac aactggcgac gctgtgcccc
    34021 ccattccagc cacagcagta acaggagcaa agcgatgaat gttgctacca gccccattgt
    34081 cagactgttg atgagtgctt cactattgat cgtcgactga tccgcgagga tcgccagcag
    34141 aaccacacac agcacaccgg ttaagggtaa aaaactcgcc agcgtagtgc ctggtaataa
    34201 aggtgtggca ggcttgcgaa tgccatctac gcggggaata gtgcgccgcc agctgcgcca
    34261 tagcagatag ctcagcaaaa cgtaggcggc gagtaacagc atcagcagca gactggcgag
    34321 tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc taattactgc
    34381 cagcgtcggt ggattacctg gcccaagaat aatcgccaca tccacgaccg aaagcgacca
    34441 ggcaacaatc gccagcatcg ccattgccag cgaaggcgcg acggagggca acagcagcca
    34501 gttcaggcat tgccagcggc tg
//
LOCUS       DADLTI010000025        33380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLTI010000025 DADLTI010000000
VERSION     DADLTI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.025"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="JN755_001096"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="JN755_001096"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7379963.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /locus_tag="JN755_001097"
     CDS             complement(2999..3928)
                     /locus_tag="JN755_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7379964.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="JN755_001098"
     CDS             complement(4145..4381)
                     /locus_tag="JN755_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7379965.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JN755_001099"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JN755_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7379966.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JN755_001100"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JN755_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7379967.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JN755_001101"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JN755_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7379968.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JN755_001102"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JN755_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7379969.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JN755_001103"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JN755_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7379970.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB"
                     /locus_tag="JN755_001104"
     CDS             complement(10727..11353)
                     /gene="ipgB"
                     /locus_tag="JN755_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7379971.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JN755_001105"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JN755_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7379972.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JN755_001106"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JN755_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7379973.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="JN755_001107"
     CDS             13569..15185
                     /locus_tag="JN755_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7379974.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="JN755_001108"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="JN755_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7379975.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="JN755_001109"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="JN755_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7379976.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="JN755_001110"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="JN755_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7379977.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="JN755_001111"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="JN755_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7379978.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="JN755_001112"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="JN755_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7379979.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /locus_tag="JN755_001113"
     CDS             17710..18435
                     /locus_tag="JN755_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7379980.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="JN755_001114"
     CDS             18432..18959
                     /locus_tag="JN755_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7379981.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /locus_tag="JN755_001115"
     CDS             18901..19596
                     /locus_tag="JN755_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7379982.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /locus_tag="JN755_001116"
     CDS             19589..19996
                     /locus_tag="JN755_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7379983.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="JN755_001117"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="JN755_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7379984.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="JN755_001118"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="JN755_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7379985.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="JN755_001119"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="JN755_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7379986.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="JN755_001120"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="JN755_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7379987.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="JN755_001121"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="JN755_001121"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7379988.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spaK"
                     /locus_tag="JN755_001122"
     CDS             26029..26430
                     /gene="spaK"
                     /locus_tag="JN755_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7379989.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /locus_tag="JN755_001123"
     CDS             26434..27726
                     /locus_tag="JN755_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7379990.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spaM"
                     /locus_tag="JN755_001124"
     CDS             27819..28175
                     /gene="spaM"
                     /locus_tag="JN755_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7379991.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="JN755_001125"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="JN755_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7379992.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spaO"
                     /locus_tag="JN755_001126"
     CDS             29016..29897
                     /gene="spaO"
                     /locus_tag="JN755_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7379993.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spaP"
                     /locus_tag="JN755_001127"
     CDS             29902..30552
                     /gene="spaP"
                     /locus_tag="JN755_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7379994.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="JN755_001128"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="JN755_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7379995.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spaR"
                     /locus_tag="JN755_001129"
     CDS             30827..31597
                     /gene="spaR"
                     /locus_tag="JN755_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7379996.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spaS"
                     /locus_tag="JN755_001130"
     CDS             31604..32632
                     /gene="spaS"
                     /locus_tag="JN755_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7379997.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="JN755_001131"
     CDS             32645..32887
                     /locus_tag="JN755_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7379998.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10425 a         4842 c         6673 g        11440 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa
//
LOCUS       DADLTI010000026        33271 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLTI010000026 DADLTI010000000
VERSION     DADLTI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.026"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JN755_001132"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JN755_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7379999.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JN755_001133"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JN755_001133"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7380000.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JN755_001134"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JN755_001134"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7380001.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JN755_001135"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JN755_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7380002.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JN755_001136"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JN755_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7380003.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /locus_tag="JN755_001137"
                     /pseudo
     CDS             complement(3421..4089)
                     /locus_tag="JN755_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JN755_001138"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JN755_001138"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7380004.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JN755_001139"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JN755_001139"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7380005.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JN755_001140"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JN755_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7380006.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JN755_001141"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JN755_001141"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7380007.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JN755_001142"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JN755_001142"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7380008.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JN755_001143"
     CDS             8207..9070
                     /locus_tag="JN755_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7380009.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JN755_001144"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JN755_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7380010.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JN755_001145"
     CDS             10406..11023
                     /locus_tag="JN755_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7380011.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVITGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JN755_001146"
     CDS             complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JN755_001146"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7380012.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12559..12816)
                     /locus_tag="JN755_001147"
     CDS             complement(12559..12816)
                     /locus_tag="JN755_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380013.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12956..13579
                     /gene="gmk"
                     /locus_tag="JN755_001148"
     CDS             12956..13579
                     /gene="gmk"
                     /locus_tag="JN755_001148"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7380014.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13634..13909
                     /gene="rpoZ"
                     /locus_tag="JN755_001149"
     CDS             13634..13909
                     /gene="rpoZ"
                     /locus_tag="JN755_001149"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7380015.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13928..16036
                     /gene="spoT"
                     /locus_tag="JN755_001150"
     CDS             13928..16036
                     /gene="spoT"
                     /locus_tag="JN755_001150"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7380016.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16043..16732
                     /gene="trmH"
                     /locus_tag="JN755_001151"
     CDS             16043..16732
                     /gene="trmH"
                     /locus_tag="JN755_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7380017.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16738..18819
                     /gene="recG"
                     /locus_tag="JN755_001152"
     CDS             16738..18819
                     /gene="recG"
                     /locus_tag="JN755_001152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7380018.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JN755_001153"
     CDS             complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JN755_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7380019.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20338..21729
                     /gene="xanP"
                     /locus_tag="JN755_001154"
     CDS             20338..21729
                     /gene="xanP"
                     /locus_tag="JN755_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7380020.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSAVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21850..23559
                     /locus_tag="JN755_001155"
     CDS             21850..23559
                     /locus_tag="JN755_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7380021.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JN755_001156"
     CDS             complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JN755_001156"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7380022.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25940..27323)
                     /locus_tag="JN755_001157"
                     /pseudo
     CDS             complement(25940..27323)
                     /locus_tag="JN755_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27616..27710
                     /locus_tag="JN755_001158"
     tRNA            27616..27710
                     /locus_tag="JN755_001158"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27650..27652,aa:Sec,seq:tca)
     gene            27982..29194
                     /locus_tag="JN755_001159"
                     /pseudo
     CDS             27982..29194
                     /locus_tag="JN755_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            29606..30649
                     /locus_tag="JN755_001160"
     CDS             29606..30649
                     /locus_tag="JN755_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7380023.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31164..31625
                     /locus_tag="JN755_001161"
     CDS             31164..31625
                     /locus_tag="JN755_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380024.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(31622..32477)
                     /locus_tag="JN755_001162"
                     /pseudo
     CDS             complement(31622..32477)
                     /locus_tag="JN755_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32474..32773)
                     /locus_tag="JN755_001163"
     CDS             complement(32474..32773)
                     /locus_tag="JN755_001163"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7380025.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
BASE COUNT         8323 a         8753 c         8212 g         7983 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaatcacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt
    12601 tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag
    12661 cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg
    12721 ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga
    12781 cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc
    12841 aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt
    12901 gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc
    12961 tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca
    13021 ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg
    13081 ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt
    13141 taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta
    13201 tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga
    13261 tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt
    13321 tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag
    13381 cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga
    13441 atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat
    13501 tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag
    13561 caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg
    13621 gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    13681 ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga
    13741 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    13801 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    13861 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg
    13921 gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga
    13981 ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac
    14041 acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga
    14101 gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac
    14161 tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga
    14221 gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa
    14281 ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc
    14341 cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat
    14401 cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat
    14461 taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat
    14521 caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc
    14581 ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa
    14641 gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat
    14701 ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg
    14761 gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc
    14821 aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt
    14881 tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca
    14941 ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg
    15001 gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag
    15061 cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt
    15121 cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg
    15181 tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac
    15241 cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct
    15301 gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa
    15361 gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg
    15421 taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc
    15481 aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc
    15541 gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct
    15601 gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc
    15661 tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc
    15721 ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga
    15781 taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg
    15841 cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa
    15901 tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg
    15961 tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac
    16021 ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct
    16081 cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt
    16141 ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc
    16201 tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt
    16261 gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat
    16321 tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc
    16381 gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc
    16441 ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc
    16501 ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg
    16561 tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc
    16621 ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg
    16681 cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg
    16741 aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt
    16801 agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc
    16861 ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat
    16921 gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg
    16981 acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg
    17041 gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc
    17101 ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg
    17161 ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc
    17221 acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa
    17281 ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg
    17341 caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa
    17401 cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc
    17461 ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc
    17521 ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag
    17581 catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt
    17641 aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca
    17701 ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt
    17761 gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg
    17821 tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc
    17881 ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt
    17941 tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg
    18001 catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc
    18061 gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc
    18121 gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact
    18181 gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg
    18241 caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc
    18301 ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa
    18361 ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac
    18421 gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg
    18481 cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg
    18541 ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg
    18601 attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg
    18661 ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt
    18721 cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa
    18781 cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg
    18841 ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    18901 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    18961 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    19021 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    19081 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    19141 catcggcagt gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag
    19201 gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    19261 gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    19321 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    19381 gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc
    19441 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    19501 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    19561 cgttgcacag gccatcgcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    19621 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    19681 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    19741 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    19801 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    19861 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    19921 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    19981 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    20041 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    20101 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    20161 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    20221 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    20281 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg
    20341 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    20401 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    20461 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    20521 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    20581 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    20641 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    20701 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    20761 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    20821 tctgccgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt
    20881 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    20941 gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    21001 gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    21061 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    21121 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    21181 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    21241 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    21301 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    21361 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    21421 ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc
    21481 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    21541 gagccgctga accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc
    21601 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    21661 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    21721 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    21781 ttcccacctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    21841 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    21901 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    21961 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    22021 gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc
    22081 ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat
    22141 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    22201 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    22261 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    22321 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    22381 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    22441 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    22501 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    22561 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa
    22621 gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    22681 cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac
    22741 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    22801 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    22861 acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc
    22921 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    22981 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    23041 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    23101 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    23161 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    23221 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt
    23281 gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg
    23341 aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag
    23401 cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa
    23461 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt
    23521 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    23581 actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt
    23641 gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta
    23701 ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    23761 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag
    23821 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    23881 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    23941 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    24001 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    24061 ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa
    24121 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    24181 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    24241 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    24301 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    24361 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    24421 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    24481 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    24541 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    24601 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    24661 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    24721 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    24781 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    24841 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    24901 cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    24961 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    25021 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    25081 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg
    25141 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    25201 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    25261 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    25321 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    25381 ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac
    25441 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    25501 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    25561 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    25621 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    25681 gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa
    25741 taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac
    25801 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    25861 cgaatacctg aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc
    25921 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    25981 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    26041 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    26101 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    26161 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    26221 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    26281 cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    26341 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    26401 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    26461 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    26521 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    26581 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    26641 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    26701 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga
    26761 ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    26821 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    26881 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    26941 tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt
    27001 ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc
    27061 agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc
    27121 aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt
    27181 ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt
    27241 acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta
    27301 acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga
    27361 taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt
    27421 atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat
    27481 atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa
    27541 aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg
    27601 tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt
    27661 tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct
    27721 tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg
    27781 tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg
    27841 tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac
    27901 ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg
    27961 gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc
    31741 ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca
    31801 cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct
    31861 tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga
    31921 cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg
    31981 ctgtcattcg cggtgacatc gaccagccaa tgacggcgcg tgaccacagg tcgatgacta
    32041 ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct
    32101 ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca
    32161 tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga
    32221 cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga
    32281 gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc
    32341 cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata ccagccgctg
    32401 cgggcaaccc gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttcg
    32461 ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatgg
    32521 acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca gcggccagtt
    32581 cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag gcataaagct
    32641 gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt tcagcgagtt
    32701 tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg ctgattgata
    32761 ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct gtgtccacta
    32821 ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat atatcatctg
    32881 taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt gaatattata
    32941 actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac
    33001 caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca
    33061 aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca
    33121 tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt agttactcaa
    33181 ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca aagattgaaa
    33241 aattatccaa cctatggttt ttattaccca a
//
LOCUS       DADLTI010000027        33128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLTI010000027 DADLTI010000000
VERSION     DADLTI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.027"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..223
                     /locus_tag="JN755_001164"
     CDS             <1..223
                     /locus_tag="JN755_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7380026.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            397..1166
                     /locus_tag="JN755_001165"
                     /pseudo
     CDS             397..1166
                     /locus_tag="JN755_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1199..2719)
                     /locus_tag="JN755_001166"
     CDS             complement(1199..2719)
                     /locus_tag="JN755_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7380027.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2810..3529
                     /gene="tonB"
                     /locus_tag="JN755_001167"
     CDS             2810..3529
                     /gene="tonB"
                     /locus_tag="JN755_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7380028.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="JN755_001168"
     CDS             complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="JN755_001168"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7380029.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4072..4611)
                     /locus_tag="JN755_001169"
     CDS             complement(4072..4611)
                     /locus_tag="JN755_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7380030.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4641..5384)
                     /locus_tag="JN755_001170"
     CDS             complement(4641..5384)
                     /locus_tag="JN755_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7380031.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5412..5501)
                     /locus_tag="JN755_001171"
                     /pseudo
     CDS             complement(5412..5501)
                     /locus_tag="JN755_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5741..6379
                     /gene="ompW"
                     /locus_tag="JN755_001172"
     CDS             5741..6379
                     /gene="ompW"
                     /locus_tag="JN755_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7380032.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6439..6945)
                     /locus_tag="JN755_001173"
     CDS             complement(6439..6945)
                     /locus_tag="JN755_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7380033.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6991..7491)
                     /locus_tag="JN755_001174"
     CDS             complement(6991..7491)
                     /locus_tag="JN755_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7380034.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7577..7756)
                     /locus_tag="JN755_001175"
     CDS             complement(7577..7756)
                     /locus_tag="JN755_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7380035.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="JN755_001176"
     CDS             complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="JN755_001176"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7380036.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="JN755_001177"
     CDS             complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="JN755_001177"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7380037.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10148..11506)
                     /gene="trpCF"
                     /locus_tag="JN755_001178"
     CDS             complement(10148..11506)
                     /gene="trpCF"
                     /locus_tag="JN755_001178"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7380038.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="JN755_001179"
     CDS             complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="JN755_001179"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7380039.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="JN755_001180"
     CDS             complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="JN755_001180"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7380040.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            14940..15821
                     /gene="yciV"
                     /locus_tag="JN755_001181"
     CDS             14940..15821
                     /gene="yciV"
                     /locus_tag="JN755_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7380041.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15818..16438
                     /locus_tag="JN755_001182"
     CDS             15818..16438
                     /locus_tag="JN755_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7380042.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16539..17411
                     /gene="rluB"
                     /locus_tag="JN755_001183"
     CDS             16539..17411
                     /gene="rluB"
                     /locus_tag="JN755_001183"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7380043.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="JN755_001184"
     CDS             complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="JN755_001184"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7380044.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18038..18796)
                     /locus_tag="JN755_001185"
     CDS             complement(18038..18796)
                     /locus_tag="JN755_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7380045.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19016..20065
                     /gene="sohB"
                     /locus_tag="JN755_001186"
     CDS             19016..20065
                     /gene="sohB"
                     /locus_tag="JN755_001186"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7380046.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20101..20352)
                     /locus_tag="JN755_001187"
     CDS             complement(20101..20352)
                     /locus_tag="JN755_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7380047.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20732..23329
                     /gene="topA"
                     /locus_tag="JN755_001188"
     CDS             20732..23329
                     /gene="topA"
                     /locus_tag="JN755_001188"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7380048.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23539..24513
                     /gene="cysB"
                     /locus_tag="JN755_001189"
     CDS             23539..24513
                     /gene="cysB"
                     /locus_tag="JN755_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7380049.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24808..24972
                     /locus_tag="JN755_001190"
     CDS             24808..24972
                     /locus_tag="JN755_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7380050.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24975..25142
                     /locus_tag="JN755_001191"
     CDS             24975..25142
                     /locus_tag="JN755_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380051.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25256..25351
                     /locus_tag="JN755_001192"
     CDS             25256..25351
                     /locus_tag="JN755_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380052.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25515..28190
                     /gene="acnA"
                     /locus_tag="JN755_001193"
     CDS             25515..28190
                     /gene="acnA"
                     /locus_tag="JN755_001193"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7380053.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="JN755_001194"
     CDS             complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="JN755_001194"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7380054.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29014..29778
                     /gene="pgpB"
                     /locus_tag="JN755_001195"
     CDS             29014..29778
                     /gene="pgpB"
                     /locus_tag="JN755_001195"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7380055.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29927..30235
                     /gene="lapA"
                     /locus_tag="JN755_001196"
     CDS             29927..30235
                     /gene="lapA"
                     /locus_tag="JN755_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7380056.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30242..31411
                     /gene="lapB"
                     /locus_tag="JN755_001197"
     CDS             30242..31411
                     /gene="lapB"
                     /locus_tag="JN755_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7380057.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31604..32341
                     /gene="pyrF"
                     /locus_tag="JN755_001198"
     CDS             31604..32341
                     /gene="pyrF"
                     /locus_tag="JN755_001198"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7380058.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32341..32667
                     /gene="yciH"
                     /locus_tag="JN755_001199"
     CDS             32341..32667
                     /gene="yciH"
                     /locus_tag="JN755_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7380059.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="JN755_001200"
     CDS             complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="JN755_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7380060.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         8399 a         8004 c         8420 g         8305 t
ORIGIN      
        1 tccacagttt tcacctgaag cattaaataa tctgaaaggt cgagtggaaa cattcacaca
       61 agatttaaag gttatcaaac cgtcagtaaa tattcagcct cagacaatca atatcacaac
      121 tcagacggta cttgatggaa aggttattga tgaaagaacc acatctcata ttaatcgtat
      181 gcaggaagat accttgatta gctcagctta cccagaggaa taaacaatgt tagaactact
      241 aatatttatt gccacacatg gcaatctatt aatctttatc tagtatgacg attcaactat
      301 gaatagtcga atgctacgtg atttcttctg agcagatacc aacatcaaaa attagatttc
      361 acgtagctca cgcttaacag aataggaata acaacaatgt ttgacacaaa gcaaattaga
      421 tactcaggta aagatggtat ctattttcac cttagagata atgtagaatc ttttcttgcc
      481 ttaacagcta ctgaacagat ggactatgac tcaccgatga tggtgacaac caggctatgc
      541 aatcggggca aactatcact gataatgttc agagagcacc taagaccatc tctattagcg
      601 gtgttgtagt ggttgattcg acgggttcag ttctattaac cagacagggg caacttgtag
      661 agacctttat ttctacggtt gaacaatggc gagaccagaa gcaaatcatc actattgttt
      721 gtaaagatgg aatatctatc actgatagta tcattactca gtttaaagcg agtaaagagc
      781 ataccattgc taatggtctt cgtattcaga tgaccttcca aggtgtagaa tttcgttcaa
      841 tcgtagggca aactgatatt tcagcagcta ctggcaagac cgctaccacg aatgatggtg
      901 gtgctaccag taaaaagaac actggcaaca ctacaacgag tcttggaagc cctatgctga
      961 cctgtaaaga gctttttagc tattcggcaa gtgagttatc agatgaagcg ctaaaagccc
     1021 gtgtaacgtg ctctaagagc gttagcgtta aaaatggtga gagcaccttc acagcctcag
     1081 ctaatgagcg agcttcaaaa gtgcttaagt cgggtaatgc tttacggaag tactctgtga
     1141 atccgaataa gaagggcact tattgagaca taaaaagggg cttcatagcc cctgaaaatt
     1201 acagttgata agcatcaata tccagtagct ctttatcagc aatttcaaag attggtgacc
     1261 catcatttag cggtagcggg tctgtaggta tggtgatttc agtgtcgttc tgtagcgtta
     1321 tagttactga acccttaccc aatgtaagcc cttttagatg tttggacaaa attaattgac
     1381 actcgacacg acccgcttta gttgtcaggt caatatcttt aagctggggt agttccttat
     1441 cagtgagtga agccttacgc ttcatagtat caatcgtggt ctgtagttct gtgcgcttct
     1501 ggttaagttc cttaagctga atttttagtt catcaatatc atccatctca agaagtaggg
     1561 cggtagcttt cttgattctg gctgtgacaa tttcaacctc atgctgtaac gctgcaatct
     1621 tcctctctga ttctccatca ttccccacgt caatcatccc cagaaaatta tcaatcatcc
     1681 atcggtcaaa cggtttacgc ttcaacggtg gaacatcaca gcgaccgtct aagtggttat
     1741 tgcacctgta gaccccagca tacttattct ttgttccggt aggatggaca ctagccccac
     1801 actgacacct aaacagacct ttgaagatgt taatcgttag tgggttgtca cttgcaggtg
     1861 tacggccttt acgattctta tcaagaatct cttgagcttt attgaacgtt gcagcatcta
     1921 tagcggcttc gtagtagttc ggtatagcgg ggcggtcatt gcgctgtgat gctggtagtg
     1981 tacctatcgt tgtctctgac cttaagatag tacgcacaga atgaacgcta aatccattag
     2041 catctttacg tcgtgagaat gaactaatgt tattagcctg taggtgacgt gcaatgttat
     2101 gcagactttc accatctagc agcagcttga aaatcaagcg tacagtttca gcacgttcat
     2161 tgagtttaaa agtctcaggg tcaatccatc cgggaacatt ccttgttaaa cgttgttcac
     2221 cagcaagggc aagctttgca cgttgttccc atttctgact aacacggaaa gacttctgtt
     2281 tactctcctg atgcgctatg tagcttttaa cccgctgaat gattgagatt cctaaatcat
     2341 cggatagagc atcccttgtg tagattagcc catcctgtag agtgtgaatc tcgataccat
     2401 gagcaataat gcgattcatc aaggcttctg tttcaagatg ttcaaggcgg cctatgcggt
     2461 cgatagcttc gactagtaaa attgtccctt ctggaaactc ccctgattca atgcgggaga
     2521 gcatatcccc aaattgccct gataagtgtt tacctttgta accggataga ccttcatcat
     2581 tgaaagatgc cactacctga gcatcgctat tcttccttaa ccactgtttg aatagctcat
     2641 tttgtcgtct ggttgaatca gcaccttcag caccttgtgt ggctgatgag aatctcaagt
     2701 atgttattac ggttctcacg gtgataaact cccatgcaat tgattatcat ttaaagatga
     2761 acttataaac gttatggcta atgctacagt gccgaatagc tacaatacaa tgccccttga
     2821 tttacctcgc cgcttcccct ggccgacgtt actttcggtc tgcattcatg gtgctgttgt
     2881 ggcgggtctg ctctatacct cggtacatca ggttattgaa ctacctgcgc ctgcgcaacc
     2941 aatttctgtc acgatggttg cgcctgctga tctcgaaccg ccacaagccg ttcagccgcc
     3001 accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac cgccaaaaga
     3061 agcaccggtg gtcattgaaa agccgaagcc gaaacctaag ccaaaaccga agccggtgaa
     3121 aaaggtacag gagcagccaa aacgcgatgt caaacccgta gagtcgcgtc cggcatcacc
     3181 gtttgaaaat acggcacccg cgcgccccac atcaagtacg gcaacggctg caaccagcaa
     3241 gccggttacc agtgtggctt caggaccacg cgcattaagc cgtaatcagc cgcagtatcc
     3301 ggcacgagca caggcattgc gcattgaagg gcaggttaaa gttaaatttg acgtcacgcc
     3361 ggatggtcgc gtggataacg tacaaatcct ctcagccaag cctgcgaaca tgtttgagcg
     3421 tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg ggtaagccag gcagtgggat
     3481 tgtggtgaat atcctgttta aaattaacgg caccaccgaa attcagtaag cagaaagtca
     3541 aaagcctccg accggaggct tttgactatt actcaacagg taaggcgcga ggttttcctt
     3601 caggatcaac cgcgacatac ttaaataatg cttctgtcgc tttatagcgt tgcccgattg
     3661 gttcagacgc tacttttttc acccacactt caatattaat gctgaccgat gtcgtccctt
     3721 tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc cgtaagaaag
     3781 tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttt gccagaatag
     3841 cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca ttggcattgg
     3901 tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg ttatgtgttg
     3961 tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact atgattttcc
     4021 tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg gttaggattt
     4081 atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga agattaaggt
     4141 aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca gccagaatgc
     4201 gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaggcca gatttagctt
     4261 cgaccatacc ggttgcggca gcgtgagttc tttacccagc atccgctgaa ttagcggctt
     4321 tttcatcacc cattggctga ctaacagggc taccgcaaac agagcataaa tgactgtaac
     4381 cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga agacgaccac
     4441 cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc agctatagat
     4501 aagcacaatc gccgtggcga cgatcagcgc tgcagtagcc gcatagatgt cataaatctt
     4561 gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca ttttacgatt
     4621 ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa cagatagatg
     4681 agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc aatttccggg
     4741 gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag caaccagctc
     4801 agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact gctacgcatc
     4861 gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc cagtaaaatg
     4921 gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa aagggtagtc
     4981 agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat agctcgcagc
     5041 gcacagactc tctgacccgc agacaccagc tggataatta atattacgcc tccggcgaga
     5101 atggcgttac cgattaattc tgaaaaagtg gacgccgctg aagcctgcag caaaatttgt
     5161 tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc gctaacgggc
     5221 acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg ccctaacacc
     5281 actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg attacggaag
     5341 aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc tccttgagtt
     5401 ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt ggcagcatca
     5461 ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc ttcaggcgtg
     5521 ggcaaaccgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata tttaaaaatt
     5581 gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc agatcaataa
     5641 atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc caatgtaggt
     5701 atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt taacagtggc
     5761 ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag caggcgaatt
     5821 ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggta gtacgttagg
     5881 aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt ttacttatat
     5941 ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc ataaaatcgg
     6001 cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac tgatggcgca
     6061 gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg gtattaacta
     6121 caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc tttccgatct
     6181 cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc tgattaaccg
     6241 tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg ccaattataa
     6301 gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc
     6361 agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg ggtcaataag
     6421 gacatggtat aaagggtatt atttcttcgc ttctacgcaa tcagtttcag aacgaattaa
     6481 aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt gcttttcctc
     6541 atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt ttgctgctgc
     6601 taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc taatagagcc
     6661 tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg cagccatttt
     6721 actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa tagtttccag
     6781 ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc gtagttcagg
     6841 ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct tttccattgc
     6901 gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa tgtcctcttt
     6961 ataaaatgac taaaagttaa aattcatatt tcaggcttta ttttcggctt tcttatttac
     7021 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtttc
     7081 tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg ctaatgtccc
     7141 gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg
     7201 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
     7261 tgccacacat ttcatgcgct taattttcag atttgattct gattccacaa cttgatcaat
     7321 tcgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
     7381 atttgatgtt gctcttgcga gttttgccag tgcccggggt aattgttttt ctgcgctgta
     7441 ggtatctgaa agcagttgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
     7501 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
     7561 aataattaac ggtgaatcag gatttgtctg atttattacc accgctttgt tgaccgcctt
     7621 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
     7681 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
     7741 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
     7801 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
     7861 agaagataga gggaaaatat attttgagga acattctgga tatattaaca attacctgag
     7921 gaataaatga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     7981 tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc
     8041 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     8101 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     8161 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     8221 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     8281 ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag cgttgtactc
     8341 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     8401 gcccgctcgt gacagcaaat atgtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     8461 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc gcaacgcggc
     8521 ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg aatcgacgcc
     8581 gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     8641 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     8701 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg
     8761 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     8821 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     8881 aggaatgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     8941 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     9001 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     9061 agcgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     9121 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     9181 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     9241 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     9301 taaatgccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc gataccgtga
     9361 ccaccaggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     9421 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     9481 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acggacaatg
     9541 gtcggataag gatgcgggcc agccgcggtg cccagcatat agtgcgcggt ttcataactg
     9601 ccggaccagt cgcgtagcgc ctcattacag gcatctttca gcgtggcgga accgctatgt
     9661 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttca
     9721 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     9781 gacgccacgc catgctgacc ggccccggtt tcggcgatga tttcggtttt acccatccgc
     9841 ttcgccagca acgcctgccc cagcacctgg ttggttttat gtgcgccgcc gtgcagcaaa
     9901 tcttcacgct tgagatacag cgtggtgttc gtcccggctg taatgttctg gcatttggtc
     9961 agtgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgggc ttgaaattca
    10021 ggatcttttt gcgcgctgac aaaagcttct tccagctggc gcagagcagg catcaggatt
    10081 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
    10141 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
    10201 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
    10261 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
    10321 catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
    10381 atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
    10441 agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc tgatcttcat
    10501 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
    10561 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
    10621 cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
    10681 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
    10741 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
    10801 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
    10861 cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
    10921 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccta
    10981 atgcaatggc gcgctccagt tcctcttcat tactgacttc ggtcagcacc cccatctcca
    11041 gactgtgagc gacggctgca agctggcgat attggtcgtc atccagtact gaaagcatta
    11101 ataagcaggc atcggcctgg taatagcgcg ccagatagat ctggtaaggg tcgataatga
    11161 agtctttaca taaaatcggc tgcggggcga tttggctgac gatgaggagg aaatcaaagc
    11221 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
    11281 tggcggcaat gcgtgccgga tcgaaatcat cacggatcac gccttttgac ggcgacgctt
    11341 ttttacactc cagaataaac gccgtgcgtg caccttgtag cgcatcataa aaatgtcgtg
    11401 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
    11461 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
    11521 ccgccagcgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
    11581 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
    11641 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac
    11701 ggttttcttc aggtgttccg cctgccagtt gctcctggtg gtagggagtc aggccaaaat
    11761 cttcagcggt caattgatag ctcttaattt tgccgtcatg cagctcggca acgattgtcg
    11821 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
    11881 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
    11941 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
    12001 ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatat ttcggcgcaa
    12061 agaggaaaca tacgcctaac tcgtccagcg cctggcgcga tttattggcg ttcatatcaa
    12121 gattaatacc gaatgccgcc agcagatccg acgagccgga tttactggag acgctgcggt
    12181 taccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
    12241 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
    12301 ggcgcgggaa cggcgctgcg ttttccagta gcgcggttgc tgccccggcg atctcgttcg
    12361 ggtgctcacc acgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
    12421 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
    12481 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
    12541 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
    12601 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
    12661 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
    12721 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
    12781 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
    12841 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
    12901 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
    12961 tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
    13021 ccacgttatg accattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
    13081 tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
    13141 agcgcgcagt acagcgcggg ctttgttacg ggtttcgtcg gcttccgact gcggaacaga
    13201 atcaaggact acaccagcac ccgcttgcac ggtggcgata ccgttttcca ccagcgccga
    13261 gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc
    13321 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg
    13381 cgcaccgctt aacgtcccca tattcataca ggcgcgataa gcgtgcaggg cgtcaagatc
    13441 gtgacgcagt tcgccgacta cgcgagagac gaggtgcatc acataggaat aacggtcaac
    13501 tttggtgaga tcggcgacgt agcggctgcc aggggtgcaa atgcgtgcca gatcattacg
    13561 ggcgagatca accagcatca gatgttcaga cagctcttta tggtcagtac gcatttccag
    13621 ttcaatacgg ctgtcgagat ctctgtccag tgaaccatcg gcgcgacgac cgcgtgggcg
    13681 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcatcatact tgagcgagct
    13741 ttccggcgac gcgccaaata gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
    13801 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
    13861 acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg
    13921 cactacgcca ccgaactctt catcgctctg attacattca caacgcatat gcggcacgga
    13981 aaccactggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt tcaggcgagc
    14041 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tacgggtgct
    14101 ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga aatcagggca
    14161 gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt cataagagaa
    14221 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca gattctgcaa
    14281 taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcgg gcgtcttcat ccagcagtgg
    14341 actgacaggg gggaagcgca gcacacggca gtttggtgat tgttcacttt ccacacccgc
    14401 aggcagggcg ttatccagta gtgccagcag ggcttcgcca ttgccggaaa gtgcctgaat
    14461 tgtgacggtg tcacctaatg ctgtaatgtg cagcgcattg tctatcagca gcaggctttt
    14521 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgattccc
    14581 acacaactgg tgaaaaagcg cagtcgggtt gtcgcgataa gcgccttcgc aggttagcag
    14641 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc
    14701 gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc
    14761 aggaagtggc caccaacctt tcagtacgaa aattgctttc attgtcgata ccatttttac
    14821 gtgaacttgc gtactagtca actggttcga tgattaattg tcaacagctc atttcagaat
    14881 atttgccaga cccgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct
    14941 tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca gcttccgatg
    15001 gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg
    15061 cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa atttcacgtt
    15121 cgggactggc gctgaatctt attcccggcg tggaaatttc cacggtctgg gaaaatcatg
    15181 aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt gagttcctcg
    15241 cgcagcagac agaacggcgc aatcagcggg cgcagcaaat tgccgaaaga cttgaaaaag
    15301 cgcaaatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc gcagtgacgc
    15361 gcggtcattt tgcgcgtttc cttgtggagt gcggcaaagc cagttcaatg gcagatgtct
    15421 ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg tgtacaatag
    15481 aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct catcctgggc
    15541 ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc gaacaccacg
    15601 gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgcacc cagctggcca
    15661 cacttgcgcg tcagcatcat ttatgggcat cacaaggatc tgattttcat cagccatgcc
    15721 cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc gtctggcagc
    15781 tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt tttatattca
    15841 tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg gagatcgtgc gtaaaggcgg
    15901 ggtgattgtc tatccaactg attccggcta tgcgctcggc tgtaaaattg aagataaaaa
    15961 cgccatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact ttaccctgat
    16021 gtgtcgcgat ctttctgaac tctcgaccta ttcattcgtg gataacgttg cgtttcgttt
    16081 gatgaaaaac aacacgccgg gcaactatac cttcatcctg aaggggacga aggaagtgcc
    16141 acgccgtctg ttgcaggaga aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat
    16201 tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc taatgctgcc
    16261 aggcagcgaa tttaccgaat ccgatcccga agaaattaaa gaccgtctgg aaaaacaggt
    16321 agatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta ttgatcttac
    16381 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataagt
    16441 gcgtaactct gtatactacg cggccttaaa atggcgctat acggcgccat tgattctcct
    16501 gttcgacgcc tgtgaaggcg acaccaaaag gaagctctat gagcgaaaaa ttacagaaag
    16561 tgctggcccg cgctggccac ggctcccgcc gtgaaatcga atctattatt gaagccggtc
    16621 gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt acccctgggc
    16681 tgaaaattcg tatcgatggt catctgattt cggtacgtga gtccgctgaa caaatttgtc
    16741 gcgttctggc ctattacaaa ccggaaggtg agttgtgtac ccgtaacgat ccggaagggc
    16801 gtccgacggt gtttgaccgt ctgccaaagc tgcgtggcgc acgctggatt gccgtaggtc
    16861 gtctggacgt taacacctgt ggtctgctgc tgttcaccac cgatggtgaa ctggcaaacc
    16921 gtttaatgca tccaagccgt gaagttgaac gtgaatacgc cgtgcgtgta tttggtcagg
    16981 ttgacgacgc gaaactgcgt gatttgagtc gtggcgtgca actggaagat ggtccggcag
    17041 cttttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac aacgtgactc
    17101 tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggt gtgcaggtga
    17161 gccgcctgat ccgtgttcgt tacggtgata tcccactgcc aaaaggtctg ccacgcggtg
    17221 gctggacgga gctggacctc gcccagacta actatctgcg cgaactggtg gagcttccgc
    17281 cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag gctaatcaga
    17341 ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcgg tcgtcgttct ggcgggcgta
    17401 ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatca
    17461 atccctattt gcgccttaac accggcatca aacgcatgtc tgatggggcg cagctcactt
    17521 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
    17581 tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac
    17641 gccaccatat acgtcagttc atcaagcaaa accatatcca gtgaggaatc agcaagcatc
    17701 cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta
    17761 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg
    17821 cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga
    17881 ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
    17941 aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct
    18001 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
    18061 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
    18121 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
    18181 aggcgcttgc gcgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
    18241 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
    18301 catatgcacc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
    18361 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
    18421 taacgttgac ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc attgggcaaa
    18481 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacgacaa
    18541 tgcgctgtgc cagttgttgg caattttcgg acgtgcaggt cagcaaatcg agaataaacc
    18601 actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
    18661 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
    18721 gaccaatccc atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
    18781 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt
    18841 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
    18901 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
    18961 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
    19021 attgttgtct gaatatggtt tgtttttggc taaaatcgtt accgttgtgc tagcgattgc
    19081 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
    19141 acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct
    19201 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga
    19261 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaacctcg
    19321 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
    19381 agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
    19441 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
    19501 tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat
    19561 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
    19621 ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
    19681 actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa ataccgaaga
    19741 agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt
    19801 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
    19861 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
    19921 tagcctgatg gaaggccgtg aagtggttaa tgtacgctat atgcagcgta aacgactcat
    19981 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac gctggtggca
    20041 gcggggtcaa aagccattga tgtaaaagac aaacgcgagg ctaagacctc gcgttttgct
    20101 ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
    20161 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
    20221 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
    20281 atgctcacga atgattttgt tagcctcttt caacagcgtt tcgcggtcga tgggttgtgt
    20341 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
    20401 agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc
    20461 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
    20521 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
    20581 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaagggggt gatatccgca
    20641 gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
    20701 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
    20761 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag
    20821 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
    20881 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
    20941 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga
    21001 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
    21061 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
    21121 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
    21181 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
    21241 tatggaccgc gtggtggggt atatggtttc gccgctgctg tggaaaaaga tcgctcgtgg
    21301 tctgtctgcc ggtcgtgtgc agtcggtggc ggtccgcctg gtggtagagc gtgagcgtga
    21361 aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga ctacgccatc
    21421 tggtgaagcg ttggcgttgc aggtgactca tcagaatgac aaaccgttcc gtccggtcaa
    21481 caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
    21541 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
    21601 acaagctgcc agcacccgtc ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg
    21661 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
    21721 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
    21781 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc
    21841 ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact
    21901 gtaccagcta atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc
    21961 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
    22021 ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt
    22081 accggcagtc gataaaggcg atgctctgac gctcgttgaa ctaacaccag cccagcactt
    22141 taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgtgg
    22201 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt
    22261 gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
    22321 tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct
    22381 cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
    22441 tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa
    22501 ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac
    22561 agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg
    22621 caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
    22681 tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
    22741 cagctatctc attgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
    22801 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
    22861 gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc
    22921 ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc
    22981 accgaaagaa gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt
    23041 cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg
    23101 tgaaacgcgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa
    23161 actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg
    23221 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
    23281 ctggtcagca ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct
    23341 atcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattttt
    23401 tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag cacctttttt
    23461 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
    23521 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
    23581 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
    23641 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
    23701 cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg
    23761 tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg
    23821 ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc
    23881 tttattgagc gttatccccg cgtttctttg catatgcacc agggcccgcc gacacaaatt
    23941 gctgatgccg tctctaaagg caatgccgat ttcgctatcg ccacagaagc gctgcatctg
    24001 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
    24061 gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg
    24121 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca
    24181 gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa aacttacgtc
    24241 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
    24301 gaccttgtgc gcgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
    24361 cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
    24421 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga aattgaggtc
    24481 atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc ggcacctttt
    24541 atgtagcgaa ggtgccggaa tatattctct tttgttactt atttctatac cgtgcacaat
    24601 atttttatcc ctcatattta tagggtaaat tacctgtaaa gtagtgtcta attacccgcc
    24661 tcgcaaattc cagttttcaa atatctattt caggtcaaaa gattgaatta ttcactggag
    24721 acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat
    24781 gattaattac accaatcatt caacgtcttg ctgataagtg atggcgatta tatgaggtta
    24841 gcaatgcctt ctggaaatca ggaaccccgc cgtgatcctg aattaaaacg taaagcctgg
    24901 ctgacggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct gatttggaaa
    24961 gtgtggggat aactatggtc ggtcaggagc aactggagtc gtcaccatta tgccagcata
    25021 gtgacaatga accagaaacg aaacgggaat gttccgtcgt tattccagac gactggcaac
    25081 taacatcgca gcagcaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat
    25141 aattatcatc attcttatta cccattttta atgaattaaa gggcttttaa tacaccgcag
    25201 caataacagc ttgagttatc tcaacacaaa ataataaccg ttaagggtgt agcctatgat
    25261 caacacaaat atgaaatatt ggtcctggat gggcgcgttt tctctgtcga tgctcttctg
    25321 ggccgaactc ctctggatca ttactcactg atccttgacc ccgctgcggc ggggttgtca
    25381 tttgctttgc cacaaggttt ctcctctttt atcaatttgg gttgttatca aatcgttacg
    25441 cgatgtttgt gttatcttta atatttaccc tgaagagaat cagggcttcg caaccctgtc
    25501 attaaggagg agctatgtcg tcaaccctac gagaagccag taaggacacg ttgcaggcca
    25561 aagataaaac ttaccactac tacagcctgc cgcttgctgc taaatcactg ggcgatatca
    25621 cccgtctacc caagtcactc aaagttttgc tcgaaaacct gctgcgctgg caggatggta
    25681 actcagttac cgaagaggat atccacgcac tggcgggatg gctgaaaaat gcccatgctg
    25741 accgtgaaat tgcctaccgc ccggcaaggg tgctgatgca ggactttacc ggcgtacctg
    25801 ccgttgttga tctggcggca atgcgcgaag cggttaaacg cctcggtggc gatactgcaa
    25861 aggttaaccc gctctcaccg gtcgacctgg tcattgacca ctcggtgacc gtcgatcgtt
    25921 ttggtgatga tgaggcattt gaagaaaacg tacgcctgga aatggagcgc aaccacgaac
    25981 gttatgtgtt cctgaaatgg ggaaagcaag cgttcagtcg gtttagcgtc gtgccgccag
    26041 gcacaggcat ttgccatcag gttaacctcg aatatctcgg caaagcagtg tggagcgaat
    26101 tgcaggacgg tgaatggatt gcttatccgg atacactcgt tggtactgac tcgcacacca
    26161 ctatgatcaa cggccttggc gtgctggggt ggggcgtggg tgggatcgaa gcagaagccg
    26221 ccatgttagg ccagccggtt tccatgctta tcccggacgt agtgggcttt aaacttaccg
    26281 gaaaattacg tgaaggtatt accgccacag acctggttct cactgttacc caaatgctgc
    26341 gcaaacatgg cgtggtgggg aaattcgtcg aattttatgg tgatggtctg gattcactac
    26401 cgttggcgga tcgcgccacc attgccaata tgtcgccaga atatggtgcc acctgtggct
    26461 tcttcccaat cgatgcagta accctcgatt acatgcgttt aagcgggcgc agcgaagatc
    26521 aggtcgagtt ggtcgaaaaa tatgccaaag cgcagggcat gtggcgtaac ccgggcgatg
    26581 aaccgatttt taccagtatg ttagaactgg atatgaatga cgttgaagcg agcctggcag
    26641 ggccaaaacg cccacaggat cgcgttgcac tgcccgatgt accaaaagca tttgccgcca
    26701 gtaacgaact ggaagtgaat gccacgcata aagatcgcct gccagtcgat tatgttatga
    26761 acggacatca gtatcagtta cctgatggcg ctgtggtcat tgctgcgata acctcgtgca
    26821 ccaacacctc taacccaagt gtgctgatgg ccgcaggctt gctggcgaaa aaagccgtaa
    26881 ctcttggcct caagcggcaa ccgtgggtca aagcgtcgct ggcaccgggt tcgaaagtcg
    26941 tttctgatta tctggcaaaa gcgaaactga caccgtatct cgacgaactg gggtttaacc
    27001 ttgtgggata cggttgtacc acctgtattg gtaactctgg gccgctgccc gatcctatcg
    27061 aaacggcaat caaaaaaggc gatttaaccg tcggtgcggt gctgtccggc aaccgtaact
    27121 ttgaaggccg tatccatccg ctggttaaaa ctaactggct ggcctcgccg ccgctggtgg
    27181 ttgcctatgc gctggcggga aatatgaata tcaacctggc ttctgagcct atcggccatg
    27241 atcgcaaagg cgatccggtt tatctgaaag atatctggcc atcggcacaa gaaattgccc
    27301 gtgcggtaga acaagtctcc acagaaatgt tccgcaaaga gtacgcagaa gtttttgaag
    27361 gcacagcaga gtggaaggaa attaacgtca cacgatccga tacctacggt tggcaggagg
    27421 actcaaccta tattcgctta tcgcctttct ttgatgaaat gcaggcaaca ccagcaccag
    27481 tggaagatat tcacggtgcg cggatcctcg caatgctggg ggattcagtc accactgacc
    27541 atatctctcc ggcgggcagt attaagcccg acagcccagc gggtcgatat ctacaaggtc
    27601 ggggtgttga gcgaaaagac tttaactcct acggttcgcg gcgtggtaac catgaagtga
    27661 tgatgcgcgg caccttcgcc aatattcgca tccgtaatga aatggtgcct ggcgttgaag
    27721 gggggatgac gcggcattta cctgacagcg acgtagtctc tatttatgat gctgcgatgc
    27781 gctataagca ggagcaaacg ccgctggcgg tgattgccgg gaaagagtat ggatcaggct
    27841 caagccgtga ctgggcggca aaaggacctc gtctgcttgg aattcgtgtg gtgattgccg
    27901 aatcgtttga acgaattcac cgttcgaatt taattggcat gggcatcctg ccgctggaat
    27961 ttccgcaagg cgtgacgcgt aaaacgttag ggctaaccgg ggaagagaag attgatattg
    28021 gcgatctgca aaatctacaa cctggcgcga cggttccggt gacgcttacg cgcgcggatg
    28081 gtagccagga agtcgtaccc tgccgttgtc gtatcgacac cgcgacggag ttgacctact
    28141 accagaacga cggcattttg cattatgtca tccgtaatat gttgaagtaa caagtatttg
    28201 cttgccggtt attttgcttc cggcaagcaa atgaattaca caatgcaaga gggttatttg
    28261 ttcagcaaat ggcccatttt ctcggctttg gtatcgagat aatgttcgtt attggggtta
    28321 cgacctacaa tcaatggtac gcgttcaaca atattaatcc ctgcttcggt cagaatttcg
    28381 acttttttcg ggttattggt taacaagcgg acttcattga cgccaaggag tttgaacata
    28441 tcagcgcaaa gagtgaagtc gcgctcatca gcggcgaagc ctaactggtg gttagcctct
    28501 acggtatcgt aaccttgatc ctgcagtgcg taagcgcgga ttttattcag cagaccaatg
    28561 ttacgacctt cctgacggtg atacagcaaa ataccacggc cttcctcggc aatttgcgtc
    28621 aatgccgctt cgagctggaa gccacaatcg cagcgcaagc tgaacagggc gtcaccggtc
    28681 agacattcgg aatggacgcg cgcaagtacc ggggtatgcc cggaaatatc gccatagact
    28741 agcgcgacat gatcgtgtcc ggttgccagt tcttcaaatc ccaccatcag gaaatcgccc
    28801 catggggttg gcagtttggc ttctgccaca cgtttaagct gcatgaaatt ctccagataa
    28861 tgctggttct gtattggctt attttgccat aacgagaagg ggttcaccta atcacgacgc
    28921 gtcgatcgtt cacggaatgg cacaaatctg tcaatttttc ttggaactgg cattttcagt
    28981 tatgattgtg ggacttatca aaaaggagag gccatgcgtt cgattgcccg acgtaccgca
    29041 gtgggagctg cactattgtt tgtcatgcca gtagccgtat ggatttctgg ctggcgttgg
    29101 caacctggag aacaaagttg gcgactaaaa gcggcttttt gggttactga aactgtcacc
    29161 cagccctggg gcgtcattac acatttgatt ttgttcggct ggtttctctg gtgtctgcgt
    29221 tttcgcatta aggctgcctt tgtattattt tccattctgg cggcggcaat ccttgttgga
    29281 caaggcgtta aatcctggat caaagacaaa gtacaggaac cacgaccttt tgttatctgg
    29341 ttggaaaaaa cacatcatat tccggttgat gagttctaca ctttaaagcg agcagaacgc
    29401 ggaaatctag taaaagaaca gttggctgaa gagaaaaata ttccacaata tttgcgttca
    29461 cactggcaga aagagacggg gtttgccttt ccttccggtc acacgatgtt tgctgccagt
    29521 tgggcactgc tggccgttgg tttgctgtgg ccgcgtcggc gaacgttaac cattgctatc
    29581 ttgttggtct gggcaacggg agtcatggga agccgcctgc tactcgggat gcattggcca
    29641 cgcgatctgg tggtagctac gttgatttcg tggacgctgg tggcggtggc aacgtggctt
    29701 gcgcaacgaa tttgtgggcc attaacacca cctgcggaag aaaatcgcga aattgcacaa
    29761 cgagaacaag aaagttaacg ctggttgatt ttccgaattt aggccttaaa tcatcaacaa
    29821 tgcgcgtgga tgccatttcg cagacggcgt gaaaatggta ctttaaaggg ctattgcggt
    29881 aagttgacca taatttattc gctctaacca cataacggga agtaatgtga aatatttact
    29941 cattttctta ctggtgttag cgatcttcgt gatttcggtc acgttgggtg cgcagaacga
    30001 tcaacaggtg acgtttaatt atctgttagc gcaaggggag taccgtattt ccacattgct
    30061 ggcggtattg tttgctgcgg ggtttgctat cggttggttg atttgtggcc tgttctggct
    30121 gcgagttcgt gtttccctgg cgcgcgctga acgtaaaata aagcgactgg aaaaccagct
    30181 ttcacccgcg actgacgtgg ctgtagtgcc gcactcgtca gcggcgaagg aataactttc
    30241 tatgctggag ttgttgtttc tgcttttgcc tgtagccgct gcctatggct ggtatatggg
    30301 ccgcagaagt gcgcaacaaa acaagcaaga tgaagccaac cgcttgtcgc gtgattacgt
    30361 agcgggggtt aacttcctgc ttagtaatca acaggataaa gcggtagacc tgtttctcga
    30421 tatgcttaaa gaggatacag gcaccgttga agcccacctt acgctcggaa acctgttccg
    30481 ttcgcgtggc gaagttgatc gcgctattcg catccatcag accctaatgg aaagcgcctc
    30541 gctgacctat gaacagcgtc tgttggcgat tcaacaactg gggcgtgatt acatggccgc
    30601 cgggttatac gaccgcgcgg aagacatgtt caatcagctg accgatgaaa ctgacttccg
    30661 cattggcgcg ctgcaacagt tgctacaaat ctaccaggct accagtgagt ggcagaaagc
    30721 aattgatgtt gccgaacgcc tggtgaagct gggtaaagat aaacagcgcg tcgaaattgc
    30781 ccatttctac tgtgagttag ccctgcagca tatggccagc gacgatctcg atcgtgccat
    30841 gaccttgcta aaaaaagggg cggcggcaga taaaaacagc gcccgcgtat ccatcatgat
    30901 gggacgcgtg tttatggcga aaggagaata cgccaaagcc gtcgaaagtt tgcaacgcgt
    30961 catatcccag gacagagaac tggtcagcga aacgctggaa atgttgcaaa cctgctatca
    31021 gcagttgggt aaaactgccg aatgggcaga attcctgcaa cgcgcggtgg aagagaacac
    31081 cggtgccgat gctgaattga tgcttgctga tatcatcgaa gcgcgcgacg gtagtgaggc
    31141 tgcacaggtc tatattacac gccagcttca gcgtcatccg accatgcgtg tattccataa
    31201 gctaatggat taccacttaa atgaagcgga agaagggcgc gccaaagaga gtctgatggt
    31261 gctgcgtgac atggttggcg agaaggtgcg tagtaagcct cgttatagct gccagaaatg
    31321 tggttttacc gcatacaccc tctactggca ttgtccgtct tgtcgtgcct ggtcaactat
    31381 taaaccgatt cgcggtcttg atggcctgta atttttaaaa aaatccgact ttagttacaa
    31441 catactagtt attgatgtcc cattgtgctc cggcaacgac ggagcagaaa aagcctgcta
    31501 ggggaacaat cgcaactgtt aattttttat ttccacgggt agaatgctcg ccgtttacct
    31561 gtttcgcgcc acttccggtg cccatcatca agaaggtctg gtcatgacgt taactgcttc
    31621 atcttcttcc cgcgctgtta cgaattctcc tgtggttgtt gcccttgatt atcataattg
    31681 tgatgacgcg ctgtcctttg tcgacaagat cgacccacgc gattgtcgtc tgaaggtcgg
    31741 taaagagatg tttacattgt ttgggccaca gtttgtgcgc gaacttcaac agcgtggttt
    31801 tgatatcttt cttgacctga aattccacga tatccccaac actgcagcgc acgctgtcgc
    31861 tgctgcagct gacttaggcg tgtggatggt gaatgctcat gcctccggtg gggcgcgtat
    31921 gatgaccgca gcgcgtgagg cactggttcc gtttggcaaa gatgcaccgc ttttgattgc
    31981 tgtgacagtg ttgaccagca tggaagccag cgacctggcc gatcttggcg tgacactgtc
    32041 acctgcagat tatgcagaac gtctggcagc actgacgcaa aaatgtggcc ttgatggtgt
    32101 ggtgtgttct gctcaggaag ctgtgcgctt taaacaggta ttcggtcagg agttcaaact
    32161 ggttacaccg ggcatccgtc cgcaggggag tgaagctggt gaccagcgcc gcattatgac
    32221 gccagaacag gcgttggcgg ctggtgttga ttatatggtg attggtcgcc cggtaacgca
    32281 atcggtagat ccagcgcaga cactgaaagc gatcaacgcc tctttacagc ggagtgcatg
    32341 atgagtgatt ccaacagccg tctggtctac tcaacggaaa ccgggcgtat tgatgagccc
    32401 aaagcggccc cagtacgccc taaaggcgat ggagcggtgc gtattcagcg tcagaccagt
    32461 ggacgtaaag gtaagggcgt ttgcctgatt accggtatcg atctcgatga tgccgaactg
    32521 acaaaacttg cagcggaact gaagaaaaaa tgcggctgcg gcggagcagt taaagatgga
    32581 gttattgaaa tccagggcga taagcgtgat ttattaaaat cactacttga agcgaaaggg
    32641 atgaaggtaa aactcgcagg cggttaacat aaaaagccac ggatatatcc gtggctttcg
    32701 aatattttac tgtgtgtatt attcagttat ttcgtactga acagaatcaa gcgtattata
    32761 attgcgagtc gataatcgac atttatttac gattatttac cgacctggtg accaataaca
    32821 ccaccgacag ctgcaccacc taatgtaccc aacgtactgc catcggtcag tactgcaccg
    32881 cctaatgccc ctgcacccgc gccgattgcg gtgttgcggt cccgtttaga ccagttagaa
    32941 caggcactca gagacattgc caaagtaatt gccagaacag ccgcggtcat ttttttgctc
    33001 gttacaaaca taatactctc tcctgaattt atgattcacg gaagtaagct ctctataact
    33061 ataatagcta agaataagcc tggtgaaatt attccgtgaa atctgctcgc gcgcagggtt
    33121 atatcacg
//
LOCUS       DADLTI010000028        32717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLTI010000028 DADLTI010000000
VERSION     DADLTI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.028"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..869)
                     /locus_tag="JN755_001201"
     CDS             complement(<1..869)
                     /locus_tag="JN755_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7380061.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLP"
     gene            complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JN755_001202"
     CDS             complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JN755_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7380062.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JN755_001203"
     CDS             complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JN755_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7380063.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JN755_001204"
     CDS             complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JN755_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7380064.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JN755_001205"
     CDS             complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JN755_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7380065.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JN755_001206"
     CDS             complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JN755_001206"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7380066.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7484..8719
                     /gene="moeA"
                     /locus_tag="JN755_001207"
     CDS             7484..8719
                     /gene="moeA"
                     /locus_tag="JN755_001207"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7380067.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8719..9468
                     /gene="moeB"
                     /locus_tag="JN755_001208"
     CDS             8719..9468
                     /gene="moeB"
                     /locus_tag="JN755_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7380068.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JN755_001209"
     CDS             complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JN755_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7380069.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10440..11339
                     /locus_tag="JN755_001210"
     CDS             10440..11339
                     /locus_tag="JN755_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7380070.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11345..13777
                     /locus_tag="JN755_001211"
     CDS             11345..13777
                     /locus_tag="JN755_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7380071.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13923..14738
                     /gene="ybiV"
                     /locus_tag="JN755_001212"
     CDS             13923..14738
                     /gene="ybiV"
                     /locus_tag="JN755_001212"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7380072.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14890..16155
                     /locus_tag="JN755_001213"
     CDS             14890..16155
                     /locus_tag="JN755_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7380073.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16396..17988)
                     /locus_tag="JN755_001214"
     CDS             complement(16396..17988)
                     /locus_tag="JN755_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7380074.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18207..19127
                     /gene="ldtB"
                     /locus_tag="JN755_001215"
     CDS             18207..19127
                     /gene="ldtB"
                     /locus_tag="JN755_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7380075.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19185..20303)
                     /locus_tag="JN755_001216"
     CDS             complement(19185..20303)
                     /locus_tag="JN755_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7380076.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20300..20767)
                     /locus_tag="JN755_001217"
     CDS             complement(20300..20767)
                     /locus_tag="JN755_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7380077.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20953..21081
                     /gene="mntS"
                     /locus_tag="JN755_001218"
     CDS             20953..21081
                     /gene="mntS"
                     /locus_tag="JN755_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7380078.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21353..22936
                     /locus_tag="JN755_001219"
     CDS             21353..22936
                     /locus_tag="JN755_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7380079.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JN755_001220"
     CDS             complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JN755_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7380080.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23552..23617)
                     /locus_tag="JN755_001221"
     CDS             complement(23552..23617)
                     /locus_tag="JN755_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380081.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23852..24739
                     /gene="rhtA"
                     /locus_tag="JN755_001222"
     CDS             23852..24739
                     /gene="rhtA"
                     /locus_tag="JN755_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7380082.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25038..25541
                     /gene="dps"
                     /locus_tag="JN755_001223"
                     /gene_synonym="pexB"
     CDS             25038..25541
                     /gene="dps"
                     /locus_tag="JN755_001223"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7380083.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25945..26691
                     /gene="glnH"
                     /locus_tag="JN755_001224"
     CDS             25945..26691
                     /gene="glnH"
                     /locus_tag="JN755_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7380084.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26830..27489
                     /gene="glnP"
                     /locus_tag="JN755_001225"
     CDS             26830..27489
                     /gene="glnP"
                     /locus_tag="JN755_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7380085.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27486..28208
                     /gene="glnQ"
                     /locus_tag="JN755_001226"
     CDS             27486..28208
                     /gene="glnQ"
                     /locus_tag="JN755_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7380086.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28630..30693
                     /gene="ybiO"
                     /locus_tag="JN755_001227"
     CDS             28630..30693
                     /gene="ybiO"
                     /locus_tag="JN755_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7380087.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JN755_001228"
     CDS             complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JN755_001228"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7380088.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31892..32152
                     /locus_tag="JN755_001229"
     CDS             31892..32152
                     /locus_tag="JN755_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7380089.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32417..>32717
                     /locus_tag="JN755_001230"
     CDS             32417..>32717
                     /locus_tag="JN755_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7380090.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         8021 a         8736 c         8098 g         7862 t
ORIGIN      
        1 ggtagataaa aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag
       61 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
      121 gaatcaataa tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt
      181 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
      241 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
      301 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
      361 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
      421 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
      481 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
      541 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      601 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      661 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      721 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      781 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      841 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      901 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      961 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
     1021 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
     1081 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
     1141 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
     1201 tctggtgtcg gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc
     1261 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
     1321 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
     1381 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa
     1441 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1501 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1561 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata
     1621 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1681 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1741 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1801 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1861 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1921 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1981 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     2041 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta
     2101 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa
     2161 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     2221 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     2281 ttaaaacgtc gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc
     2341 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc
     2401 agcccagttc aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg
     2461 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     2521 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2581 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2641 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2701 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2761 gcccggcaat caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     2821 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2881 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg
     2941 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     3001 cgggatccac ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     3061 ttccgccgga tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     3121 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag
     3181 tgcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     3241 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     3301 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     3361 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     3421 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3481 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3541 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3601 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3661 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3721 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3781 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3841 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3901 accaatctcc ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg
     3961 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     4021 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     4081 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     4141 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     4201 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     4261 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     4321 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     4381 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     4441 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4501 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4561 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4621 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4681 cgcaccgtga accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4741 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4801 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4861 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4921 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4981 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     5041 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     5101 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg
     5161 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     5221 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     5281 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     5341 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc
     5401 catccaccac cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata
     5461 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     5521 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5581 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5641 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5701 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5761 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5821 agagttgatg cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5881 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5941 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     6001 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     6061 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc
     6121 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     6181 cgcctacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     6241 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     6301 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     6361 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     6421 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcggggagt
     6481 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6541 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa
     6601 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6661 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct
     6721 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6781 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa
     6841 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6901 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6961 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     7021 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     7081 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     7141 tcgccggctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     7201 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     7261 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     7321 aatatcagaa tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc
     7381 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg
     7441 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7501 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac
     7561 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7621 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     7681 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata
     7741 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7801 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7861 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7921 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7981 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     8041 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     8101 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     8161 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     8221 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     8281 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt
     8341 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct
     8401 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     8461 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8521 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8581 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8641 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8701 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8761 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8821 ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8881 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8941 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     9001 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     9061 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     9121 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     9181 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     9241 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     9301 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     9361 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     9421 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9481 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9541 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9601 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9661 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9721 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta
     9781 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9841 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9901 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9961 cttgtgccgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
    10021 taatcgctgc cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag
    10081 aacgcagctt aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
    10141 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
    10201 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
    10261 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    10321 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    10381 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
    10441 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10501 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta
    10561 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10621 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10681 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg
    10741 gtgaagtgaa aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg
    10801 atcgcagcgg cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg
    10861 cgacggcgct gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc
    10921 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10981 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    11041 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    11101 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg
    11161 acgagctgca cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat
    11221 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc
    11281 tcgattttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    11341 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    11401 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    11461 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    11521 cctggcgaat cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag
    11581 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11641 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt
    11701 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat
    11761 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    11821 gacctccggc gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga
    11881 tggtctgcgt gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga
    11941 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    12001 cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg
    12061 tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca
    12121 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    12181 taacggtcac tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg
    12241 cgacgttgaa ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg
    12301 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc
    12361 gggaagtccg ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc
    12421 aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc
    12481 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    12541 ctgcgtacag gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt
    12601 gatcccggaa tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat
    12661 tggttgtata gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12721 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12781 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga
    12841 agtgatggcc gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    12901 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12961 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    13021 ttctggcctg caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    13081 ggtgtttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    13141 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    13201 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    13261 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    13321 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga
    13381 cgggcgtaaa gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca
    13441 tctcggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    13501 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13561 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13621 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13681 tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca
    13741 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt
    13801 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13861 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13921 ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa
    13981 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    14041 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    14101 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    14161 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    14221 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    14281 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    14341 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    14401 tagtgatcga caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt
    14461 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    14521 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14581 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14641 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14701 aggctgtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    14761 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14821 cttatttata acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc
    14881 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14941 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat
    15001 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    15061 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    15121 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    15181 caagcgctag gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag
    15241 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    15301 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    15361 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    15421 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc
    15481 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15541 cagcacgttt tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg
    15601 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15661 cggacattcc agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac
    15721 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15781 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15841 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15901 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15961 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    16021 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    16081 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    16141 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    16201 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    16261 atccggccta caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag
    16321 cgtagcgcat caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg
    16381 gtgacggata atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct
    16441 aaagtcgatc acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    16501 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    16561 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    16621 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    16681 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    16741 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    16801 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    16861 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    16921 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    16981 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    17041 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    17101 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    17161 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    17221 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    17281 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    17341 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    17401 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    17461 aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc
    17521 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    17581 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc
    17641 cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg
    17701 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc
    17761 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    17821 acccagcgtc ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc
    17881 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    17941 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    18001 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    18061 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    18121 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    18181 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    18241 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    18301 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    18361 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    18421 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    18481 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    18541 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    18601 aagatacgcc tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga
    18661 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg
    18721 ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg
    18781 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    18841 gtctacgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    18901 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    18961 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta
    19021 ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    19081 atgaagcgat caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta
    19141 aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc
    19201 ataactaaca aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga
    19261 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    19321 gatcctggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga
    19381 ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg
    19441 actgccgtta accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc
    19501 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    19561 aacgtccagt ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca
    19621 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc
    19681 cccagacaat tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat
    19741 tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg
    19801 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    19861 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    19921 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    19981 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    20041 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    20101 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    20161 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    20221 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    20281 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    20341 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    20401 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    20461 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    20521 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    20581 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    20641 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    20701 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    20761 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    20821 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
    20881 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    20941 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
    21001 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    21061 gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    21121 ggcataacgc gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc
    21181 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    21241 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    21301 attgaccctt gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt
    21361 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    21421 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    21481 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    21541 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    21601 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    21661 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    21721 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    21781 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    21841 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    21901 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    21961 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat
    22021 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    22081 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    22141 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    22201 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    22261 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    22321 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    22381 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    22441 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    22501 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    22561 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    22621 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    22681 tcagcaaaat ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt
    22741 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    22801 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    22861 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    22921 cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag
    22981 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    23041 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    23101 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    23161 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    23221 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    23281 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    23341 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    23401 tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag
    23461 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    23521 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat
    23581 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    23641 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    23701 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    23761 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    23821 tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    23881 ctggttacca atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt
    23941 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    24001 aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    24061 acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct
    24121 ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    24181 ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    24241 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    24301 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    24361 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    24421 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    24481 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    24541 cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc
    24601 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    24661 cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa
    24721 agaattagac attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt
    24781 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
    24841 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
    24901 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
    24961 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
    25021 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
    25081 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
    25141 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
    25201 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
    25261 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
    25321 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
    25381 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
    25441 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
    25501 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
    25561 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
    25621 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
    25681 gtatccacat catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta
    25741 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
    25801 atcttcaggg tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
    25861 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
    25921 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
    25981 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
    26041 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
    26101 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
    26161 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
    26221 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
    26281 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
    26341 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
    26401 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
    26461 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
    26521 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
    26581 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
    26641 ggaacttaca acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
    26701 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
    26761 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
    26821 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
    26881 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
    26941 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
    27001 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
    27061 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
    27121 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
    27181 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
    27241 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
    27301 agcattaaag acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
    27361 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
    27421 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
    27481 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
    27541 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
    27601 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
    27661 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
    27721 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
    27781 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
    27841 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
    27901 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
    27961 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
    28021 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
    28081 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
    28141 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
    28201 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
    28261 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
    28321 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
    28381 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
    28441 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
    28501 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
    28561 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
    28621 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
    28681 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
    28741 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
    28801 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
    28861 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
    28921 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
    28981 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
    29041 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
    29101 ctgaatgcag gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
    29161 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
    29221 cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta
    29281 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
    29341 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
    29401 tacctgatct tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
    29461 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
    29521 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
    29581 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
    29641 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
    29701 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
    29761 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
    29821 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
    29881 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
    29941 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
    30001 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
    30061 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
    30121 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
    30181 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
    30241 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
    30301 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
    30361 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
    30421 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
    30481 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
    30541 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
    30601 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
    30661 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
    30721 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
    30781 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
    30841 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
    30901 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
    30961 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
    31021 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
    31081 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
    31141 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
    31201 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
    31261 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
    31321 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
    31381 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
    31441 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
    31501 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
    31561 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
    31621 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
    31681 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
    31741 cagcattatg catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa
    31801 gcatatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
    31861 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
    31921 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
    31981 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
    32041 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
    32101 ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca
    32161 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
    32221 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
    32281 gtcaagtgat aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa
    32341 cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga
    32401 aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct
    32461 cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga
    32521 agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc
    32581 gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat
    32641 gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct
    32701 caaaaacgtc cccggcg
//
LOCUS       DADLTI010000029        32328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLTI010000029 DADLTI010000000
VERSION     DADLTI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.029"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JN755_001231"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JN755_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7380091.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JN755_001232"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JN755_001232"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7380092.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JN755_001233"
     CDS             complement(3736..4092)
                     /locus_tag="JN755_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7380093.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JN755_001234"
     CDS             complement(4096..4512)
                     /locus_tag="JN755_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7380094.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JN755_001235"
     CDS             complement(4623..5336)
                     /locus_tag="JN755_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7380095.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JN755_001236"
     CDS             5699..6241
                     /locus_tag="JN755_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380096.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JN755_001237"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JN755_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7380097.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JN755_001238"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JN755_001238"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7380098.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JN755_001239"
     CDS             complement(9042..10457)
                     /locus_tag="JN755_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7380099.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JN755_001240"
     CDS             10540..11523
                     /locus_tag="JN755_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7380100.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JN755_001241"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JN755_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7380101.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JN755_001242"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JN755_001242"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7380102.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="JN755_001243"
     CDS             complement(13465..14172)
                     /locus_tag="JN755_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7380103.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14196..14450)
                     /locus_tag="JN755_001244"
     CDS             complement(14196..14450)
                     /locus_tag="JN755_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380104.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="JN755_001245"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="JN755_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7380105.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="JN755_001246"
     CDS             complement(15325..15534)
                     /locus_tag="JN755_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7380106.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JN755_001247"
     CDS             complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JN755_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7380107.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JN755_001248"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JN755_001248"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7380108.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /locus_tag="JN755_001249"
     CDS             complement(17766..18134)
                     /locus_tag="JN755_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7380109.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="JN755_001250"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="JN755_001250"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7380110.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JN755_001251"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JN755_001251"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7380111.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JN755_001252"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JN755_001252"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7380112.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="JN755_001253"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="JN755_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JN755_001254"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JN755_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7380113.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JN755_001255"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JN755_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7380114.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JN755_001256"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JN755_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7380115.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="JN755_001257"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="JN755_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7380116.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="JN755_001258"
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="JN755_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY7380117.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31164..32054
                     /gene="malG"
                     /locus_tag="JN755_001259"
     CDS             31164..32054
                     /gene="malG"
                     /locus_tag="JN755_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7380118.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8228 a         8293 c         7804 g         8003 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaacttatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa cctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aacaaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attcttttgc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcaataa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta atgcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgctcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gccaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctggct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgtt actgcgtgac gtaaacagct acaccctggc cgtggggatg cagcaatacc
    31921 tcaacccgca aaactacctg tggggtgact ttgccgccgc tgccgtgatg tctgcattac
    31981 cgatcaccat cgtcttcttg ctggctcaac gctggctggt caacggcctg acggcaggtg
    32041 gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc gctgcaccct caacttacgt
    32101 tatcccaact tgtgactatt attcggcgct ccacggagcg ccttttttct ttcgtctgca
    32161 atctgaatcg ttcgccggtt aatattccca tcatagaact tattattttt acgttatttg
    32221 ttttcccact tacgataatt ctctttcgtg ctctgagtca cggcaatagt attgttttca
    32281 tcaatttttg ataattatca caattaagat cacagaaaag acattacg
//
LOCUS       DADLTI010000030        32306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLTI010000030 DADLTI010000000
VERSION     DADLTI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.030"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            63..695
                     /locus_tag="JN755_001260"
     CDS             63..695
                     /locus_tag="JN755_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7380119.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(783..2081)
                     /locus_tag="JN755_001261"
     CDS             complement(783..2081)
                     /locus_tag="JN755_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380120.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2564..3976
                     /gene="uxaC"
                     /locus_tag="JN755_001262"
     CDS             2564..3976
                     /gene="uxaC"
                     /locus_tag="JN755_001262"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7380121.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3991..5478
                     /locus_tag="JN755_001263"
     CDS             3991..5478
                     /locus_tag="JN755_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7380122.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5561..6112
                     /locus_tag="JN755_001264"
     CDS             5561..6112
                     /locus_tag="JN755_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7380123.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JN755_001265"
     CDS             complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JN755_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7380124.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JN755_001266"
     CDS             complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JN755_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7380125.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9193..9393)
                     /locus_tag="JN755_001267"
     CDS             complement(9193..9393)
                     /locus_tag="JN755_001267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7380126.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9424..10191)
                     /locus_tag="JN755_001268"
     CDS             complement(9424..10191)
                     /locus_tag="JN755_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7380127.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10270..10962)
                     /locus_tag="JN755_001269"
     CDS             complement(10270..10962)
                     /locus_tag="JN755_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7380128.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11039..11542)
                     /locus_tag="JN755_001270"
     CDS             complement(11039..11542)
                     /locus_tag="JN755_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7380129.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11627..12763
                     /gene="rlmG"
                     /locus_tag="JN755_001271"
     CDS             11627..12763
                     /gene="rlmG"
                     /locus_tag="JN755_001271"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7380130.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13048..13362
                     /locus_tag="JN755_001272"
     CDS             13048..13362
                     /locus_tag="JN755_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY7380131.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13359..13775
                     /gene="higA"
                     /locus_tag="JN755_001273"
     CDS             13359..13775
                     /gene="higA"
                     /locus_tag="JN755_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7380132.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13820..15838)
                     /locus_tag="JN755_001274"
     CDS             complement(13820..15838)
                     /locus_tag="JN755_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7380133.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JN755_001275"
                     /pseudo
     CDS             complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JN755_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JN755_001276"
                     /pseudo
     CDS             complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JN755_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(19631..21064)
                     /locus_tag="JN755_001277"
     CDS             complement(19631..21064)
                     /locus_tag="JN755_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7380134.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21127..21576)
                     /locus_tag="JN755_001278"
     CDS             complement(21127..21576)
                     /locus_tag="JN755_001278"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7380135.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JN755_001279"
     CDS             complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JN755_001279"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7380136.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JN755_001280"
     CDS             complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JN755_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7380137.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26044..26375
                     /locus_tag="JN755_001281"
                     /pseudo
     CDS             26044..26375
                     /locus_tag="JN755_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JN755_001282"
     CDS             complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JN755_001282"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7380138.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            28213..29733
                     /locus_tag="JN755_001283"
     CDS             28213..29733
                     /locus_tag="JN755_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7380139.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29887..30510)
                     /locus_tag="JN755_001284"
     CDS             complement(29887..30510)
                     /locus_tag="JN755_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7380140.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30787..31533
                     /gene="nfeF"
                     /locus_tag="JN755_001285"
                     /pseudo
     CDS             30787..31533
                     /gene="nfeF"
                     /locus_tag="JN755_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31587..31662)
                     /locus_tag="JN755_001286"
     tRNA            complement(31587..31662)
                     /locus_tag="JN755_001286"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31627..31629),aa:Ile,seq:cat)
     gene            31787..32293
                     /gene="mug"
                     /locus_tag="JN755_001287"
     CDS             31787..32293
                     /gene="mug"
                     /locus_tag="JN755_001287"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7380141.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7815 a         8429 c         8500 g         7562 t
ORIGIN      
        1 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
       61 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
      121 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
      181 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
      241 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
      301 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
      361 cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg
      421 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
      481 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
      541 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
      601 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
      661 taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt
      721 ttctatataa acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa
      781 cgttaatgtt gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg
      841 ttctgcaata cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg
      901 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
      961 cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg
     1021 aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     1081 atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca
     1141 atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     1201 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     1261 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     1321 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc
     1381 agtgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     1441 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     1501 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     1561 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc
     1621 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     1681 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     1741 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     1801 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     1861 gtacccagca catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa
     1921 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     1981 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     2041 agtgcgatca tataccaacg taacccttta attttactca tgtaaaacct catcgttttg
     2101 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     2161 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     2221 ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc
     2281 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag
     2341 agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac
     2401 tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg
     2461 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     2521 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     2581 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     2641 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     2701 ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg
     2761 taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga
     2821 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt
     2881 cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     2941 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     3001 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     3061 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     3121 ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     3181 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     3241 ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt
     3301 tgctgaagcg aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac
     3361 cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga
     3421 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     3481 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     3541 ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     3601 cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     3661 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     3721 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt
     3781 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     3841 ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     3901 caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     3961 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     4021 aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag
     4081 actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca
     4141 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     4201 gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     4261 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc
     4321 tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg
     4381 ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac
     4441 gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc
     4501 gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc
     4561 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
     4621 ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
     4681 atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc
     4741 gagccaggaa aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
     4801 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
     4861 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
     4921 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag
     4981 tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc
     5041 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc
     5101 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt
     5161 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
     5221 ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc
     5281 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
     5341 atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
     5401 gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa
     5461 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
     5521 gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc
     5581 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
     5641 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
     5701 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
     5761 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
     5821 cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac
     5881 ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg
     5941 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
     6001 ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat
     6061 tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa
     6121 ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac
     6181 agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata
     6241 aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc
     6301 agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa
     6361 gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc
     6421 gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc
     6481 agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata
     6541 ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc
     6601 aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg
     6661 ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag
     6721 ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg
     6781 atcggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg
     6841 accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac
     6901 accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc
     6961 attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta
     7021 ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca
     7081 gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc
     7141 tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc
     7201 agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc
     7261 catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga
     7321 gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt
     7381 gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt
     7441 gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag
     7501 agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact
     7561 ggctattaag gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg
     7621 cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg
     7681 ccaatcgccg ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt
     7741 tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg
     7801 cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc
     7861 ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa
     7921 tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg
     7981 ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc
     8041 cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa
     8101 caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag
     8161 caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa
     8221 cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct
     8281 caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg
     8341 cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg
     8401 caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc
     8461 ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca
     8521 gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag
     8581 cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa
     8641 gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg
     8701 tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa
     8761 cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc
     8821 atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac
     8881 agcaaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata
     8941 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
     9001 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
     9061 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
     9121 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
     9181 ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc
     9241 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
     9301 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
     9361 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac
     9421 tttctagcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
     9481 tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
     9541 catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac
     9601 gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat
     9661 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
     9721 ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt
     9781 cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa
     9841 cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga
     9901 cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga
     9961 aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat
    10021 cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg
    10081 ctcaaggctg cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc
    10141 gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg
    10201 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    10261 agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga
    10321 tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg
    10381 agcgagtttc tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt
    10441 aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa
    10501 ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga
    10561 atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc
    10621 gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact
    10681 caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg
    10741 ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac
    10801 ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat
    10861 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    10921 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    10981 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    11041 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    11101 ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct
    11161 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    11221 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag
    11281 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    11341 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    11401 agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    11461 ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct
    11521 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    11581 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    11641 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    11701 acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc
    11761 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    11821 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    11881 cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    11941 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    12001 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    12061 tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac
    12121 gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga
    12181 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc
    12241 gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc
    12301 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    12361 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    12421 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt
    12481 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    12541 ccctccgttt caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca
    12601 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga
    12661 ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa
    12721 atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc
    12781 cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata
    12841 agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa
    12901 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat
    12961 tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg
    13021 ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat
    13081 gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct
    13141 aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc
    13201 aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca
    13261 atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac
    13321 gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct
    13381 tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg
    13441 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    13501 ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg
    13561 cggaatttaa tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta
    13621 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    13681 cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    13741 gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc
    13801 cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc
    13861 gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc
    13921 tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat
    13981 tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa
    14041 tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat
    14101 ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca
    14161 tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct
    14221 gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg
    14281 attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga
    14341 tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct
    14401 tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg
    14461 tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc
    14521 ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg
    14581 ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta
    14641 cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga
    14701 ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg
    14761 cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct
    14821 ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca
    14881 gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga
    14941 gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga
    15001 cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga
    15061 tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg
    15121 cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt
    15181 cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac
    15241 gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac
    15301 gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt
    15361 cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca
    15421 gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca
    15481 atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca
    15541 gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct
    15601 ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa
    15661 tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca
    15721 gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc
    15781 ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa
    15841 aattgtccag tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga
    15901 aaagggaaaa ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta
    15961 acgccggatg cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg
    16021 ataaggcgtt cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt
    16081 tatacaacat atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc
    16141 ctgtcagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc
    16201 tgaagaacgt atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt
    16261 tcagaccaaa ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa
    16321 aagccggatt ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat
    16381 cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg
    16441 gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg
    16501 ccagtggttt atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac
    16561 aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg
    16621 gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg
    16681 cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt
    16741 tttcggcgaa tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca
    16801 actgttcttt gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg
    16861 cagcgacctg tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt
    16921 agttgttcag cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct
    16981 cgccgctctc ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc
    17041 cgttatgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct
    17101 ctgccaacca ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa
    17161 ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa
    17221 ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag
    17281 gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat
    17341 gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc
    17401 agcgtccggt caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc
    17461 agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat
    17521 ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat
    17581 aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag
    17641 cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac
    17701 tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt
    17761 ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca
    17821 ggccatcacg ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt
    17881 tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca
    17941 ccagatccag cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg
    18001 cgaagcgcag agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc
    18061 cgggaatact gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca
    18121 ggcggtcgaa attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg
    18181 gaaagccgcc catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat
    18241 cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg
    18301 caccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac
    18361 ttatcagcag agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt
    18421 ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca
    18481 tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac
    18541 cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg
    18601 gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc
    18661 atcatcccgc tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag
    18721 gcgcgagcgg caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg
    18781 gtaatttcca gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg
    18841 ttcatccatt gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag
    18901 ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt
    18961 gccgcttaag ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta
    19021 gcccaaatcg ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg
    19081 cagactgagc atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat
    19141 cccaccttcg aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc
    19201 ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag
    19261 gccaccttca atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat
    19321 atatccacca agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat
    19381 tgcgggtgtt tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa
    19441 ggcgtagcaa aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt
    19501 gttaatccat gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa
    19561 aggcagaaac tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt
    19621 attagtctgc ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg
    19681 ttaatccctt tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac
    19741 cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga
    19801 caaaccctac ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg
    19861 agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca
    19921 gcatgatgaa taacggcgga agcatggacg ctgcggcggt catattaata atggtattca
    19981 tcagatcctg cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca
    20041 gaaactggat ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct
    20101 taccaaagat gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca
    20161 tcagcaggga accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg
    20221 cttccggcag accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa
    20281 atttcaactc tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta
    20341 cgccgataaa aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga
    20401 cgtcgttaac gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg
    20461 taacgccgag gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg
    20521 caggctgtac gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca
    20581 gcagcatcag cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg
    20641 aaacccaggt ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca
    20701 taatatattc gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg
    20761 aggtgaaaaa gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc
    20821 cgcccagcga actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa
    20881 caaattctgc gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca
    20941 tcggtgccga ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa
    21001 cggcggcaaa agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag
    21061 acataacaca tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg
    21121 tagttgttat ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt
    21181 gaccgcgtta ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc
    21241 ttcgtggacc tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg
    21301 ataatattcc actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg
    21361 caggtagtaa tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga
    21421 gtcattttcc acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat
    21481 gttgtcgata ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat
    21541 ctggcggaac tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc
    21601 gtggaaaaga accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg
    21661 ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag
    21721 tccagcacct cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc
    21781 agggtgatgt catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc
    21841 tgggtatagt gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg
    21901 tcgtggcggt tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg
    21961 aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc
    22021 tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat
    22081 tcgccgttaa tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa
    22141 cgctcgccgg aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta
    22201 cagcgcatcc cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact
    22261 tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga
    22321 ttcggctgcc acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag
    22381 ttgatctttg gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg
    22441 ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga
    22501 tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg
    22561 ttttccttca gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg
    22621 cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc
    22681 gtgatctgca agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc
    22741 gcgagcgttt caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta
    22801 aaccacagtt tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc
    22861 gcgataacct gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa
    22921 ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac
    22981 cagacattgc cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa
    23041 tgaccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt
    23101 ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca
    23161 ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca
    23221 gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg
    23281 tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc
    23341 gacggatggt ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc
    23401 cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg
    23461 acgtcggttt ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga
    23521 tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga
    23581 tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg
    23641 ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg
    23701 atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc
    23761 agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg
    23821 acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg
    23881 ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag
    23941 gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca
    24001 aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca
    24061 tcgcggaaga tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc
    24121 cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca
    24181 aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact
    24241 tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg
    24301 ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca
    24361 tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc
    24421 cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct
    24481 ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac
    24541 aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag
    24601 tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata
    24661 ggacctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct
    24721 gggggggtta gtaaaatttt tactaaaagt agcagataaa tgcatttact ttaactggat
    24781 cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga
    24841 tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg
    24901 cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg
    24961 aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa
    25021 cgctgataag cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta
    25081 cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca
    25141 gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct
    25201 cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg
    25261 ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat
    25321 agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc
    25381 tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc
    25441 gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta
    25501 atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa
    25561 tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt
    25621 agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg
    25681 tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat
    25741 tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga
    25801 ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc
    25861 attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga
    25921 cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag
    25981 cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa
    26041 gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt
    26101 gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg
    26161 aaaaaacact gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg
    26221 ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg
    26281 aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg
    26341 agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc
    26401 gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac
    26461 gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc
    26521 ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc
    26581 tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac
    26641 cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca
    26701 acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg
    26761 tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg
    26821 ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca
    26881 cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc
    26941 aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg
    27001 cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta
    27061 cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt
    27121 tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc
    27181 ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc
    27241 caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg
    27301 actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga
    27361 agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc
    27421 gtagcggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg
    27481 cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt
    27541 cgataaactc ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta
    27601 cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga
    27661 catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca
    27721 gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg
    27781 aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt
    27841 attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg
    27901 ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt
    27961 tgtcgggtat ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct
    28021 gatttttggg caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc
    28081 aaagaattag atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa
    28141 gataaccgca gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca
    28201 caggacgcga acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac
    28261 gataccactc tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact
    28321 tttgtgcagg tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg
    28381 cgtcacccgg atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg
    28441 gagccctgga gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg
    28501 gccaatgcgg taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc
    28561 cgggcgacgg atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga
    28621 cgtaccagta agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg
    28681 ccttcattac cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg
    28741 gcggccatgc tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg
    28801 gtattgttgg caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc
    28861 cgtcaggcac tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc
    28921 gatgagctgg ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta
    28981 attaacgatg tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa
    29041 ggcaccgatg aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg
    29101 gcgaccatga accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc
    29161 gataaacttt ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg
    29221 gtgatcaaga caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg
    29281 ctgattaacg atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg
    29341 gcgcgtgccg gcgaacaggg caaaggtttt gcagtggtgg cgggggaagt gcgtcattta
    29401 gccagccgca gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat
    29461 aaggtgcaat ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg
    29521 gcacaggtga aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag
    29581 gccgatgggc tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa
    29641 aatgccgagc tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc
    29701 cgactggaag acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag
    29761 cacaatgcct gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt
    29821 aggccggata aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg
    29881 cggcgattaa tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    29941 ttttatttgt gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    30001 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc
    30061 tttgatgcgt tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc
    30121 ttgttcggtc agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga
    30181 ctgctcctgc agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc
    30241 ctgggttaga ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag
    30301 aatatccaga atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc
    30361 gccgcgaccg tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca
    30421 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    30481 tttacaacac ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat
    30541 ctaatttata tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag
    30601 cacctcacat ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac
    30661 tgaatgcctt cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat
    30721 taacaatcat tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg
    30781 ataacaatga ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt
    30841 gaactgactg tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc
    30901 ggcgaggcgt tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt
    30961 cctcaacctg acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg
    31021 gaaggaccac gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa
    31081 ctggcgatcg atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta
    31141 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    31201 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    31261 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    31321 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    31381 cgcgtctggc gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa
    31441 ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg
    31501 gtacgtgcag cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac
    31561 gatttctcgt cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa
    31621 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
    31681 gtaactccgt gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat
    31741 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
    31801 tttggctcca gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg
    31861 gactggtttt ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg
    31921 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
    31981 cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct
    32041 gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc
    32101 gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa
    32161 acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag
    32221 tcgcgtttca ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt
    32281 gcgtgggcga taaaaaacgc caccga
//
LOCUS       DADLTI010000031        31095 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLTI010000031 DADLTI010000000
VERSION     DADLTI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.031"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..2643
                     /locus_tag="JN755_001288"
     CDS             <1..2643
                     /locus_tag="JN755_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7380142.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVA
                     DGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDA
                     LNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQL
                     YTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANG
                     GAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNT
                     DATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSST
                     VDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQII
                     NVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVN
                     GNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDA
                     PQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQV
                     TNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVA
                     LGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADG
                     SIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNK
                     LSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRW
                     VYKLQGSTNSQGEYSAALGAGIQW"
     gene            3011..4666
                     /gene="lldP"
                     /locus_tag="JN755_001289"
     CDS             3011..4666
                     /gene="lldP"
                     /locus_tag="JN755_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7380143.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4666..5442
                     /gene="lldR"
                     /locus_tag="JN755_001290"
     CDS             4666..5442
                     /gene="lldR"
                     /locus_tag="JN755_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7380144.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            5439..6629
                     /gene="lldD"
                     /locus_tag="JN755_001291"
     CDS             5439..6629
                     /gene="lldD"
                     /locus_tag="JN755_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7380145.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            6825..7298
                     /gene="trmL"
                     /locus_tag="JN755_001292"
     CDS             6825..7298
                     /gene="trmL"
                     /locus_tag="JN755_001292"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7380146.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7351..8172)
                     /gene="cysE"
                     /locus_tag="JN755_001293"
     CDS             complement(7351..8172)
                     /gene="cysE"
                     /locus_tag="JN755_001293"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7380147.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8252..9271)
                     /gene="gpsA"
                     /locus_tag="JN755_001294"
     CDS             complement(8252..9271)
                     /gene="gpsA"
                     /locus_tag="JN755_001294"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7380148.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9271..9738)
                     /gene="secB"
                     /locus_tag="JN755_001295"
     CDS             complement(9271..9738)
                     /gene="secB"
                     /locus_tag="JN755_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7380149.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9801..10052)
                     /gene="grxC"
                     /locus_tag="JN755_001296"
     CDS             complement(9801..10052)
                     /gene="grxC"
                     /locus_tag="JN755_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7380150.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10194..10625)
                     /locus_tag="JN755_001297"
     CDS             complement(10194..10625)
                     /locus_tag="JN755_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7380151.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10870..12414
                     /gene="gpmM"
                     /locus_tag="JN755_001298"
                     /gene_synonym="pgmI"
     CDS             10870..12414
                     /gene="gpmM"
                     /locus_tag="JN755_001298"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7380152.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12424..13707
                     /gene="envC"
                     /locus_tag="JN755_001299"
     CDS             12424..13707
                     /gene="envC"
                     /locus_tag="JN755_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7380153.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13711..14670
                     /locus_tag="JN755_001300"
     CDS             13711..14670
                     /locus_tag="JN755_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7380154.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(14657..15691)
                     /gene="waaH"
                     /locus_tag="JN755_001301"
     CDS             complement(14657..15691)
                     /gene="waaH"
                     /locus_tag="JN755_001301"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7380155.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15930..16955)
                     /gene="tdh"
                     /locus_tag="JN755_001302"
     CDS             complement(15930..16955)
                     /gene="tdh"
                     /locus_tag="JN755_001302"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7380156.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16965..18161)
                     /gene="kbl"
                     /locus_tag="JN755_001303"
     CDS             complement(16965..18161)
                     /gene="kbl"
                     /locus_tag="JN755_001303"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7380157.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18436..19293)
                     /gene="yibB"
                     /locus_tag="JN755_001304"
     CDS             complement(18436..19293)
                     /gene="yibB"
                     /locus_tag="JN755_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7380158.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            19597..20529
                     /gene="rfaD"
                     /locus_tag="JN755_001305"
     CDS             19597..20529
                     /gene="rfaD"
                     /locus_tag="JN755_001305"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7380159.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            20539..21585
                     /gene="rfaF"
                     /locus_tag="JN755_001306"
     CDS             20539..21585
                     /gene="rfaF"
                     /locus_tag="JN755_001306"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7380160.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            21589..22581
                     /gene="rfaC"
                     /locus_tag="JN755_001307"
     CDS             21589..22581
                     /gene="rfaC"
                     /locus_tag="JN755_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7380161.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            22578..23786
                     /gene="rfaL"
                     /locus_tag="JN755_001308"
     CDS             22578..23786
                     /gene="rfaL"
                     /locus_tag="JN755_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7380162.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(23822..24964)
                     /locus_tag="JN755_001309"
     CDS             complement(23822..24964)
                     /locus_tag="JN755_001309"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7380163.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(24973..25986)
                     /locus_tag="JN755_001310"
     CDS             complement(24973..25986)
                     /locus_tag="JN755_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY7380164.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(26011..26718)
                     /gene="rfaY"
                     /locus_tag="JN755_001311"
     CDS             complement(26011..26718)
                     /gene="rfaY"
                     /locus_tag="JN755_001311"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7380165.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(26744..27751)
                     /gene="waaO"
                     /locus_tag="JN755_001312"
                     /gene_synonym="rfaI"
     CDS             complement(26744..27751)
                     /gene="waaO"
                     /locus_tag="JN755_001312"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7380166.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(27794..28591)
                     /gene="rfaP"
                     /locus_tag="JN755_001313"
     CDS             complement(27794..28591)
                     /gene="rfaP"
                     /locus_tag="JN755_001313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7380167.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(28584..29708)
                     /locus_tag="JN755_001314"
     CDS             complement(28584..29708)
                     /locus_tag="JN755_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7380168.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(29705..30763)
                     /gene="rfaQ"
                     /locus_tag="JN755_001315"
     CDS             complement(29705..30763)
                     /gene="rfaQ"
                     /locus_tag="JN755_001315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7380169.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8179 a         7624 c         7291 g         8001 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggcgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgctctgct ttgggatgcc
      241 accgcaggta aattcagtgc caaacacggt actaatggtg acgcaagcgt gatcactgat
      301 gtcgcagatg gtgaaatttc agactccagt tctgacgcag taaacggctc acaactccac
      361 ggcgtgagca gttatgttgt tgatgcgctg gggggtggtg ccgaagtcaa tgcagacggc
      421 accatcactg cgccgacgta caccattgct aatgctgatt acgataatgt cggtgatgcc
      481 ctgaatgcta tcgataccac tcctgacgac gctctgctct gggatgcgga cgccggtgaa
      541 aatggtgcat ttagcgccgc tcacggaaaa gataaaactg ccagtgtaat cactaacgtc
      601 gctaacggtg caatctctgc tgccagcagc gacgcgatta acggctcaca actctatacc
      661 accaataagt acatcgctga tgcgctgggt ggtgacgcag aagtcaacgc tgacggcacc
      721 atcaccgcac cgacttacac cattgcgaac gccgagtaca acaacgtcgg tgacgccctg
      781 gatgcgcttg atgataacgc cctgctgtgg gataagactg ccaatggcgg tgctggagcc
      841 tacaatgcca gccatgacgg taaagccagc atcatcacta atgtcgctaa tggcagtatt
      901 agtgaggaca gtaccgatgc agtgaacggt tctcagttga atgcgacgaa tatgatgatt
      961 gagcagaaca cccaaattat caatcagctc gctggtaaca ccgacgcaac ctatatcgaa
     1021 gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acgatttagc ctttaacgat
     1081 gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc gtctggtgcc
     1141 agcagcgtag ccattggtca gaacagcagc agcaccgttg ataccggtat tgcgctgggt
     1201 agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag cgtaacggaa
     1261 aatggcttgg tgattggtta tgacaccact gacggcgaac tgctaggtgc attgtcaatt
     1321 ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttcaga agcccatgac
     1381 gccgttacgg ttcgccagtt gcagaacgct attggagcgg tcgccactac gccaaccaag
     1441 tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga ctcgctggca
     1501 atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt gaacactctg
     1561 gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc aaaccatgca
     1621 aacagtatcg caatgggtaa cggttctcag accacccgtg gtgcacagac tggctacacc
     1681 gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgtcgg tagtgaagac
     1741 ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg ataccgatgc ggttaacgtg
     1801 ggtcagttga aagtcactga tgagcgcgta gcgcaaaata cccagagcat tactaacctg
     1861 aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg tattggcgac
     1921 attgtcacca ccggtagcac caagtacttc aagaccaaca ccgatggcgt agatgccaac
     1981 gcccagggta aagatagcgt tgctattggt tctggttcca ttgctgccgc tgacaacagc
     2041 gtcgcactgg gtaccggttc cgttgcagag gaagaaaata caatctctgt aggttcttct
     2101 actaaccaac gccgtatcac caacgtagct gccagtgtta atgccaccga tgcggttaac
     2161 gtgtcgcagc tgaaatcttc tgaagcaggc ggagtgcgct acgacaccaa agctgatggt
     2221 tctatcgact atagcaatat caccctcggt ggtggcaacg gcagtacgac tcgtatcagc
     2281 aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt gaagcaaagc
     2341 gcgcaagaaa cgaagcaata caccgatcag cggatggttg agatggataa caaactgtct
     2401 aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac cggtctgccg
     2461 caggcttata caccgggtgc cagcatggct tctattggtg gcggtactta caacggtgaa
     2521 tcggcagttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt ctacaaatta
     2581 caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg tattcagtgg
     2641 taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga tgcgttacgc
     2701 gcatcaggca agataaggcg ctccgggtca acaatctaag ggcaattctc tgatgaggat
     2761 tgcccttttc tttaccagac atctcccccc acaagaattg gccctaccaa ttcttcgctt
     2821 atctgacctc tggttcacaa ttccccaatt aaaactcaca tcaatattga taatacataa
     2881 catttagtta accattcatt gtcattatcc ctacacaaca caattggcag tgccactttt
     2941 acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg tggactctag
     3001 gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata tctggctttc
     3061 cagtctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta agctcaaact
     3121 gaaaggatac gtcgccgcct catggacggt agcaatcgcc cttgccgtgg ctttgctgtt
     3181 ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct tctatggttt
     3241 gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct cggtgaaaac
     3301 cgggcagttt gacatcattc gctcgtctat tctttcgata acccctgacc agcgtctgca
     3361 aatgctgatc gtcggtttct gtttcggcgc tttccttgaa ggagccgcag gctttggcgc
     3421 accggtagca attaccgccg cattgctggt cggcctgggt tttaaaccgc tgtacgccgc
     3481 cggactgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg gcattccaat
     3541 cctggttgcc ggacaagtaa caggcatcga cagctttgag attggccaga tggtggggcg
     3601 tcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta tggacggctg
     3661 gcgcggtatc aaagagacgt ggcctgcggt cgtggttgca ggcggctcgt ttgccatcgc
     3721 tcagtacctt agctctaact tcattgggcc ggagctgccg gacattatct cttcgctggt
     3781 atcactgctc tgcctgacgc tgttcctcaa acgctggcaa ccagttcgcg tattccgctt
     3841 cggtgacttg ggggcgtcac aggttgatat aacgctggcc cacaccggtt acaccgcggg
     3901 tcaggtgtta cgtgcctgga caccgttcct gttcctgacc gctaccgtaa cactgtggag
     3961 catcccgccg tttaaagccc tgttcgcatc gggtggtgcg ctgtatgagt gggtgatcaa
     4021 cattccggtg ccgtacctca ataaactggt tgcccgtatg ccaccagtgg tcagcgaggc
     4081 tacagcctat gccgccgtgt ttaagtttga ctggttctct gccaccggca ccgccattct
     4141 gtttgctgca ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac
     4201 cttcggcagc acgctgaaag aactagctct acccatctac tccatcggca tggtgctggc
     4261 gttcgccttt atctcgaact attccggact gtcatcaaca ctggcactgg cactggcgca
     4321 caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg tattcctgac
     4381 cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg cagcacaaca
     4441 aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg tcaccggtaa
     4501 gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga
     4561 gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt
     4621 gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat tgttttaccc
     4681 agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg
     4741 gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg cgtatcacgt
     4801 aattcactac gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc
     4861 ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa catcgtccag
     4921 ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct ggaagcccgc
     4981 tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa
     5041 gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat cgcctcacaa
     5101 gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa
     5161 accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg tcagcggatg
     5221 tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt cattgacgcc
     5281 atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct tagttttgtt
     5341 cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat tacccgcctg
     5401 cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc gcagccagcg
     5461 attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat atggatggtg
     5521 gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa gtggcactgc
     5581 gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg tttaatgaga
     5641 aattgtcgat gccggtagca ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg
     5701 gcgaagttca ggcagccaaa gcggcggacg cgcatggcat tccgtttact ctctcgacgg
     5761 tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc
     5821 tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg
     5881 gttgttcgac gctggttttc accgtggata tgccgacacc gggcgcacgc taccgtgatg
     5941 cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg ctacttgcag gcggtgacgc
     6001 atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc acatgattta ggtaatatct
     6061 cagcttatct cggcaaaccg accggactgg aagattacat cggctggctg gggaataact
     6121 tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg gatggcccga
     6181 tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt tttggtgctg
     6241 atggaattgt ggtttctaac cacggtggcc gccagctgga cggtgtactc tcttccgctc
     6301 gtgcgttgcc cgctattgca gatgcggtga aaggtgatat cgccattctg gcggatagcg
     6361 gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac accgtactgc
     6421 tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc
     6481 taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg aaatcgatca
     6541 gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct acggcactgg
     6601 ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctcaccct aaccctctcc
     6661 ctcagggaga ggggaccgtt cggcgctgta tgtactccct cactctgaaa cgacaccgca
     6721 ctcttttttt ctccctcgcc cctccgggga gggccggggt gaggggaaaa ggccgcacgg
     6781 ttcaccaatt ttctgctatc ctgcccccgc actaaggggg cagtatgcta aacatcgtac
     6841 tttacgaacc agaaattccg ccaaatactg gcaacatcat ccgtctttgc gctaataccg
     6901 gctttcgtct acatatcatc gaaccgatgg gatttgcctg ggacgacaag cgcctgcgcc
     6961 gcgcggggct ggactatcac gagtttaccg ccgttacgcg tcatcatgac tatcgcgcgt
     7021 tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt acgcctgctc
     7081 acagcgccgt aagctatcag gatggcgatt atctgatgtt cggcccggaa acacgcggcc
     7141 tgccagcgag cattcttgat gccctgcccg ctgaacaaaa aattcgcatt ccgatggtgc
     7201 cggacagccg cagcatgaat ctgtccaatg cggtgtcggt agtggtgtat gaagcctggc
     7261 ggcagctggg atatccggga gcgttgttga gagattagtt actgtatgcc ggatagatga
     7321 ttacatcgca tccggcacga tcacaggaca ttagatccca tccccatact caaatgtatg
     7381 gttaataccg ttgaaatgct ggtccatatc cattgatggc ttatcgctgt ctggtttacc
     7441 gacaatacga gccggaacgc cagcggcggt tgtatgcggc ggcaccggtt gcagcaccac
     7501 ggaacctgcg ccaatcttcg cgccgcgacc aacttcaata ttgccgagga ttttcgcgcc
     7561 cgcgccaatc atcacacctt cacgaatttt cgggtgacgg tcaccaccag atttacccgt
     7621 accgccaagc gtcacagatt gcagaatcga tacgtcgttt tcaattaccg ccgtttcacc
     7681 cacaacgatg cccgtggcgt ggtcgagcat aatgccacgg ccaatttttg ctgccgggtg
     7741 aatatcgacc tggaacgtca cagaaacctg gttttgcaga aagattgcca atgcgcgacg
     7801 cccctgattc cacaaccagt gaccgatgcg ataggcctgc aaggcatgaa aacccttcag
     7861 gtataacaac ggggttgagt atttatcgac tgccgggtcg cgggtacgca ccgcctgaat
     7921 atcacaggcc gcagaggcga tcatttccgg gtcagcggcg taggcttctt caaccacttc
     7981 acggatagca atagcaggca taattggcga tgacagcttg ttcgccagca tgtagctcag
     8041 tgcactgcca aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg
     8101 ctcacagtcc gccagcgttc tggcttcggc tttaatattg ttccagacaa tttccagttc
     8161 ttcacacgac attgcttact ccacacgatg agataatgac cggcccgttc tgcgcgggcc
     8221 gggtcatagc ggtaacaaag gttccctggg gttagtggct gctgcgctcg tccttgcgtg
     8281 cacgacctaa taatgtcaat gctgcctcgc gcgcgttttt tccgcaatat aatacttgat
     8341 aaatttcctc ggttattggc atttcaacgc cgaagcgatg cgccagttcg cggacttctt
     8401 tcgtattgcg gtagccttcc accacctgac caatcttctc ctgcgcgctt tgtacatcca
     8461 tgccctgacc gagcatcatg ccaaaacggc ggttacgcga ctggttgtcg gtacaggtaa
     8521 gcaccagatc gcccagcccc gccatgccca taaaggtggc aggatcggca cccagcgccg
     8581 caccaagacg cgacatttca gccagcccac gggtgatcag cgccgtacgc gcattcgcac
     8641 caaaaccgat accgtcagac atccccgcgc caatggcaat gacgtttttc accgcgccgc
     8701 caagctgcac gccaatgaaa tccgggttgc tgtaaacgcg gaaacttttg ccgcagtgca
     8761 gcagctgctg gagatcatcg gcaaaggtct gatcggtcga ggccagcgaa atagctgtcg
     8821 gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga gataaccgcc agcggaattt
     8881 gatcgcctaa ggcctcacgc gccacgtcct gtaacagacg tccggtttcc gcttccagcc
     8941 ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag cggtttaatc tggcgcagca
     9001 cttcaccaaa gacatggctg ggtacgacga cgagaatatt acgactggct gccagcgcag
     9061 tggcgagatc gctttcaaga tggagcgtat cgggaaaagg cacatcgggg agaaacgcgg
     9121 cgttacagcg gtcgcgttca agcgttgcga tatgttcagg gtcatggccc cagaggacaa
     9181 cctcgtggcc atttcttgcc agggtaatgg caagagcggt gccgtacgag ccggcaccga
     9241 tcacagtcat tgaagcatta cgttggttca tcaggcatcc tgatgttctt cagtaccttc
     9301 gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg gcgcaaggtt
     9361 cagttgcggg aatgtaccgc gggataccat gctggtgatg cactcacgag catacgggaa
     9421 cagaatgttc gggcagtatg cgcccaggca atgtgccatc tgggtgcctt cgatacccgc
     9481 gatggagaaa ataccgccct gctgaacttc acacaggaac gcggtttctt cgcccaagga
     9541 ggccgttacg gtaacacgca gtaccacttc gtatacgtca tctgccagtt gggaagatgc
     9601 cgtatccaga tcaagtttaa cttctggttg ccaatctttc tggaaaacgt gcggcgcgtt
     9661 cggcgcttcg aaagagatat ccttggtata aatacgttgg atctggaaag tcatttcagt
     9721 gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat actgcgcaat
     9781 gtaatgccaa cgttcacacg ttatttcagc aggggatcca gtccaccacg tgcatccaat
     9841 gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg gggaaccgtg
     9901 gtgcgaccgc tgcgtttgat catctcttca cgcttggcgg cattgccatc gatcggcaac
     9961 tcctggaaac tcacgccctt gctgctcagc agtgcttttg cacgatggca atacgggcag
    10021 gtttctttgg tatagatttc aacattggcc ataatttagc tcctgttttt ttaccctgaa
    10081 atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca gaagcttata acaataagtc
    10141 gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt aaattatttg
    10201 ccgcgcacca gaggcaagtt ttcgccagcc cagccagcaa cgccctcttt cagcacgaat
    10261 acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct cctggcactg catgccagaa
    10321 ccgtctacca cgataaccga tttgtctttg tgcttctcaa gctcaccaac attgttggct
    10381 ttgatttcgc tcggcaacag attgatagaa cctgcgatat ggcctttacg gaagtcatca
    10441 cgctgacgta aatccacaac cacagcgtct tctttgttga tcagacgcgt agcttcacca
    10501 cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag aaccgccacc
    10561 agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg cataatttct
    10621 tgcatggggg gtaacaactc ccaacgtagt gattaaaaaa accaggaaag gagtatacct
    10681 gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg caattttgcg gggcacgacg
    10741 aaaaaaaagc gcaaatctgg catcggcttg ccgcgagcgg tcgtatattt tgatcttcag
    10801 aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct tgagtatgag
    10861 gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga tggctatggc
    10921 tatcgtgaag aacagcagga taacgccatt tttagtgcta aaaccccggt aatggatgca
    10981 ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt cggtctgcct
    11041 gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg ccgcatcgtg
    11101 tatcaggacc tgactcgtct ggacgttgaa atcaaagatc gcgctttctt tgctaatcca
    11161 gtgctggctg gcgcggtaca taaagcgaaa aacgcaggta aagcagtaca cattatgggt
    11221 ctgctctccg caggcggcgt acacagccac gaagatcaca tcatggcgat ggtagaactg
    11281 gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg tcgcgacacc
    11341 ccgccgcgca gtgctgaatc ctcgctgaaa aaatttgaag agaaatttgc tgcgctgggt
    11401 aaaggccgcg tagcgtccat cattggtcgt tactacgcga tggaccgcga taaccgttgg
    11461 gatcgcgttg aaaaagccta tgacctgctg actctggcgc agggtgagtt ccaggccgat
    11521 actgccgttg ctggtttgca ggcagcttat gctcgcgacg aaaacgacga gttcgtgaaa
    11581 gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg cgatgcgctg
    11641 attttcatga acttccgtgc tgaccgcgcg cgtgaaatca ctcgtgcatt cgtgaacgct
    11701 gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat gctgaccgaa
    11761 tacgccgctg acatcaaaac tgcggttgct tacccacccg cttccctggt taacaccttc
    11821 ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta tttccgaaac cgaaaaatat
    11881 gcccacgtta ctttcttctt caacggtggc gtagaagagt cgttcaaagg cgaagatcgc
    11941 attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat gagctccgca
    12001 gagctgaccg aaaaactggt tgcggccatc aagagcggca aatacgacac cattatctgt
    12061 aactatccga acggcgacat ggtaggtcac accggggtga tggaagcggc ggttaaagcg
    12121 gttgaagcac tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc cgtgggtgga
    12181 caactgctga tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc ggcaacgggt
    12241 caggcacaca cggcacacac caacctgcca gttccgctga tttacgttgg tgataagaac
    12301 gtgaaagcgg ttgcaggcgg caaactttct gacatcgcgc cgaccatgtt gtcgctgatg
    12361 ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga ataatccctc
    12421 cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg caggtttgca
    12481 atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg cgccttttcc
    12541 gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc cgcaaaagag
    12601 cacgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt gaaaaagcag
    12661 gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac gctcaatcaa
    12721 ctcaataaac agattgatga gatgaacgcg tcgattgcca aactggagca gcaaaaagcc
    12781 gcccaggagc gcagcctcgc cgcacaactg gatgccgcat tccgtcaggg cgagcatacc
    12841 ggtattcagc taattctcag cggtgaagaa agccagcgtg gacagcgttt acaggcttat
    12901 ttcggctatc tcaaccaggc gcgacaagaa accattgccc agttgaagca aacgcgtgaa
    12961 gaagtcacca tgcagcgtgc tgaactggaa gagaaacaaa gcgagcaaca aacgctgtta
    13021 tatgaacagc gcgcccaaca ggcgaaactg actcaggttc tgaacgagcg taaaaagacg
    13081 ctggcggggc tggaatcttc catccagcaa ggtcagcagc agctaagtga actacgtgcc
    13141 aacgaatccc gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa agcgcgtgct
    13201 gaacgagaag cgcgtgaggc ccaggcggtt cgcgagcgcc agaaagaagc gacgcgcaaa
    13261 ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga tgtcccgaac tggtggcctg
    13321 ggtgcgccgc gtggtcaggc attctggccg gttcgcgggc cgacgctgca tcgctatggc
    13381 gagcagctac agggtgaatt acgctggaaa gggatggtta tcggtgcctc tgaaggtact
    13441 gaagttaaag caattgccga cggtcgagtg attctggctg actggctgca aggttacggt
    13501 ctggtggtgg tggttgagca cggtaaaggc gacatgagtc tttacggcta taatcagagc
    13561 gcactggtga gcgttggttc gcaggttcgc gcgggccagc caattgcact ggtgggcagc
    13621 agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg tcaggcggtc
    13681 aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa cgttcttgca
    13741 tttgccgctc tgttggcgct ctcctccccc gtacttgctg gcaaacttgc catcgtcatt
    13801 gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc ttccgctatc
    13861 tccgtcgctg tattacccga ttcaccgcac gccagagaga tggcgaccaa agcgcataac
    13921 agcggtcacg aagtgttgat tcatctcccg atggcaccgt tgagtaaaca gccgctggag
    13981 aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat tcgtagtgcg
    14041 gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa gatgacctct
    14101 aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct ttacttcctc
    14161 gacagcgtaa ccatcggtaa tacccaggcg atgcgcgccg cgcaaggtac tggcgtgaag
    14221 gtgatcaaac ggaaggtgtt cctcgacgat tcgcagaacg aagcggacat ccgtgtgcaa
    14281 tttaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat tgggcatcct
    14341 cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc agacattacg
    14401 ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacttctac accgccgaaa
    14461 aacgctgtgc ctgatgcacc gcgtaatccg ttccgtggcg tgaagctgtg caaaccgaag
    14521 aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga aagcatcagc
    14581 cagagcacgc tgatcgttta cttccagcat cagtggcaag gctggggcaa acagcacgaa
    14641 gcggcgaagt ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga tggtgcgacc
    14701 gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctgg taaccgacct
    14761 tcacgctgcg cacattggta atcaggcgct tatacatacc ggaagtaaat atctcggcaa
    14821 tcatccgttg gcgggtaaga atatccggct ctttgcgcac cgcatggcaa acacgcaatg
    14881 cttcgtaagt tatttgctga tgaaattccg gataaatcgt aattttgtcg gcataatttc
    14941 gatttaattt ctccagcagg cgggtaatct taatatagtg acgttgataa ttaaggtttt
    15001 tattcccttg tctatgcaac cgactcactg acgtattatg cagataatat ttatataatg
    15061 attgctcggt atatcgcgca cgcagcgcgt taaacatgaa ttctgttgtc cagacaatat
    15121 cctgatgatg taatccggca ataaatttaa tgttattttt aacaataaca tcacggcgat
    15181 aaacccccat ccagacaacg tgagtccaac ggcgcgaaga aagccccatc cgcagccagt
    15241 ccgggccggt taatacaccg gttgagcgaa ggcgatcgct ggggatggat tgccaggttt
    15301 ctcccgtttc acgaaaagac cagtcagcgt tgcactgcgc cacgtcgagg tcgtcctcta
    15361 acgccatggt catcagcgtt tcgtacatgg tgggatagac ctcatcgtca gcatcgacaa
    15421 aagcgacata tttgcccgtc gccacctcga tcccacgatt acgcgctacc gatgcgccag
    15481 cattcgcctg atgcaacaaa cgaacgtgcg gatagttttc tgcgtaatgc tttgctattt
    15541 caacagaatt atccgttgaa ccatcgttaa taataatgat ttccagagca gtccaggttt
    15601 gcgtaattaa agattccata caagtgcgga aatcatcgcc cgcattatat aacggaataa
    15661 taacactaag tttattggtg ctgttcatca taaattaccg acgacctctg aagttatcct
    15721 gacgttttac aggctttgta ttaagaaact attaagcctg tgagatatta tttttacagg
    15781 tgagagagtc acttcagggt agagagataa tgagaacagc tatataaaag atcagcgcaa
    15841 ttacgaactc tttattacac tgacaggatt ttacataatt aacagattat cctcagataa
    15901 aagggctgga ataccagccc ttgttcgtgt taatcccagc tcagaataac tttcccggac
    15961 tggcccgaac gcatagcgtc aaagcccttc tggaaatcat cgatagagaa acgatgggta
    16021 atgatcggcg agagatcgag gccagactga atcagcgccg ccatcttgta ccaagtttca
    16081 aacatctcac gaccgtaaat gcctttaatg aacaagcctt taaagatcac tttggtccag
    16141 tcgatagaca tatcagacgg tggaataccc agcatcgcaa tacgaccgcc gtggttcatg
    16201 gtatcgagca tggtacgaaa cgctggcggc gcgccggaca tttccagacc gacatcaaag
    16261 ccttcggtca tgcccagttc agccatcaca tcattaaggt tttctttcgc gacgttaacc
    16321 gcacgggtga tacccatttt gcgcgccagc tcaaggcggt attcgttaac atcggtgatc
    16381 accacattgc gtgcaccaac gtgtttcgcc accgccgcag ccataatacc tatcggacct
    16441 gcaccagaaa ccagcacatc ttcgcccacc agatcgaacg acagcgcggt atgcacggcg
    16501 ttaccgaagg gatcaaaaat ggaagccagg tcgtcggaga tattgtcggg gattttgaaa
    16561 gcgttgaacg ccggaatcac cagatattcg gcgaagcagc ccggacggtt aacaccgaca
    16621 ccaatggtgt tgcggcacag atgggtacga ccgccacgac agttgcgaca gtgaccgcag
    16681 gtgatatggc cttcgccaga aacgcgatcg ccgattttga agcctttcac ttcctgacca
    16741 atacctacca cttcaccgac atattcatgg cccacgacca tcggcaccgg aatggttttt
    16801 tgcgaccact catcccagtt atagatgtga acgtcagtcc cgcagatggc tgttttacgg
    16861 attttaatca gcagatcgtt atgcccgagt tccggtacag ggacgtcggt catccagatg
    16921 ccctcttccg ctttcagttt ggataacgct ttcatctcac atcctcaggc gataacgccc
    16981 agttgtttac caatacgcgt gaatgcttct actgcacgcg taatttgctc aggggtatgc
    17041 gccgcagaca tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg atagaagaaa
    17101 ccggtgacgt aaatgccctc tttttgcagc tctcgggcaa atttctgcgc cactaccgca
    17161 tcaccaagca tgaccggaat aatggcgtga tcggctcccg ccagggtaaa gcccgccgcc
    17221 gacatttgct cacggaactg acgcgcgttc gcccacagac ggtcacgcaa ctcgctaccc
    17281 gcttcaacca tctccagcac tttgatggaa gcggcgacaa tcgctggtgc cagcgagttg
    17341 gagaacaggt acggacgaga acgctggcgc agccactcaa ccacttcttt gcgcgccgcg
    17401 gtataaccac cagaagcccc gcccagcgct ttaccgagcg tgccggtgat gatgtcgaca
    17461 cgccccatca catcgcagta ttcatgggaa ccacgaccat tttcaccgac aaaaccgacc
    17521 gcgtgggagt cgtctaccat caccagggca tcgtatttat ctgccagatc gcaaacgccc
    17581 ttcagattgg caatcacgcc gtccatcgag aacacaccat cggtggcaat cagcacatga
    17641 cgcgcaccgg cttcacgcgc ttctttcaga cgagcttcaa gctcctgcat atcgttgttg
    17701 gcatagcgat agcgtttagc tttacacaga cgcacaccat caataataga cgcgtggttc
    17761 agtgcgtcgg agataatggc gtcttccgcg cccagcagcg tttcaaacag gccaccgtta
    17821 gcatcaaagc aggaagagta gagaatcgca tcttccatcc ccaggaatgc cgccagtttt
    17881 tgttcaagct ctttatggct gtcctgagtg ccgcaaataa aacgcaccga agccatgccg
    17941 aaaccgtgag aatccattcc cgcctttgct gccgcaatca gatccggatg attcgccagc
    18001 ccgagatagt tgttggcaca aaagttaatg acgtggcttc catcagccac ggtgatatct
    18061 gcttgctgcg cagacgtaat aatgcgctct tctttaaaca acccttccgc ccgtgcggtt
    18121 tccagatcgt tggttaactg ctgataaaat tctccacgca ttgcgattct ccagacaggg
    18181 caaattccag cacatattac ccaaacttat aggtcgcgac gagatgatgc gttaacactt
    18241 ctgcaaaatt caggataaat aacgtgtatc ttgatgtctc tcaacaccta cagatagtag
    18301 aaaaactgta cagttttggc tgagcataat gatgagtgat tttactgcat tgatataaaa
    18361 agaaaaaatg atgccaaagc atcatttttc ctatctcagt tggcatcaaa acgctaccca
    18421 ctaatggagg gaaacctatt ttcttgaaag gaaaattctc agcgcctgca tttttgcccc
    18481 taaagtgtta ctcctgaagc agcgaaacaa atcgaaccat tttcctctcc ccagatagtt
    18541 taggttgaag agatcggaac gtttgtaata gcacatgaca aaaatgccct ggtcatcgtc
    18601 cacaatgtta ttgtttagtg ttattttttg actttccaga actaatttat agaattcttt
    18661 ccatttatgc tgtgaaccaa caattgcgcc cccaataata tatacatgat tgccaatcat
    18721 gaaatcaaaa gcctgttgtt gagatgtaac agtaagccct ttttttatag tgaaaagatg
    18781 catcttattt tcatcaaatg ggaaatccca aattttcaat cctcgagtta catttggctt
    18841 acggcaatag ccaaaatcta tccacgcaac aagaggcgtt ttcactaaac ccatgttgat
    18901 agctttattt acaaaatagg acttaaggtt acataccaaa acatactctg gtgaccagta
    18961 ctctgggttt tttaattgtc gaggctcaag tctatttgta aacgactcat ctttttgaat
    19021 tttttcgata cgacttctga tatatctaaa tttctttttt atatcgataa cgataaccgt
    19081 tgttggtttt ccgttgcgaa tcgcttcaat ccttggtttc aaatcgggag aagtgaaaat
    19141 aatcatctca ttttcaagtg cggccagacg ctcaaagtaa ctaaaataaa catcaactga
    19201 acgagctagt ttttcacgga accctttatt agtagtccaa tcccctctac caatgtcaaa
    19261 atatgcagtg ataattgtgg tggatgattt cattactaat aagaccatta atataaaaaa
    19321 agaattatat cataaatatt aacagaatgt atactgtata ttatccattc aaatgattcc
    19381 gttatttatc atacatcgga atattgatac taaagcacta ttcacatgca aaaccaacat
    19441 ccgccatgaa ggactagcta aaacccaaac tagtttgttg caattagcat ccttgcacct
    19501 ctatgtaaag ggctgaaggg attcggatgt gatggtatga ttacagacat tcgtgtctga
    19561 gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac cggcggcgcg
    19621 ggctttatcg gcagcaacat cgttaaggcc ctgaatgata aaggcatcac cgatattctg
    19681 gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct ggatatcgcg
    19741 gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga gttcggtgat
    19801 gtcgaagcga ttttccacga aggcgcatgc tcttccacca ccgagtggga tggcaagtat
    19861 atgatggata acaactatca atactccaaa gagctgctgc actactgtct ggagcgcgaa
    19921 atcccgttcc tgtatgcctc ttccgcagcc acctacggcg gacgcacctc cgactttatt
    19981 gaatcccgcg agtacgaaaa accgttgaat gtctacggtt actcaaaatt cctgtttgat
    20041 gaatatgttc gtcaaatcct gccagaagcg aactcgcaga ttgttggctt ccgctatttc
    20101 aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc tttccatctc
    20161 aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga gaacttcaaa
    20221 cgtgacttcg tctatgtggg cgacgtggca gatgtaaacc tgtggttcct ggaaaatggc
    20281 gtttccggta tcttcaacct cggcaccggt cgtgcagaat ccttccaggc agtagcagat
    20341 gctacgctgg cttatcacaa gaaaggccag atcgaataca ttccgttccc ggataagctg
    20401 aaaggccgct accaggcgtt cactcaggca gatctgacaa atctgcgcgc ggcgggttac
    20461 gacaaaccgt tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg gctgaatcgc
    20521 gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt tggcgacatg
    20581 atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc gataatcgat
    20641 gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt taacgaagct
    20701 atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa actgggtcat
    20761 agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac caaactcctt caaatctgca
    20821 ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga gatgcgctac
    20881 ggtttactca acgatgtacg cgtgctcgat aaagaagcct ggccgctaat ggtggaacgc
    20941 tatgtcgcgc tggcctatga caaaggcatt atgcgtaccg cacaagatct gccgcagcca
    21001 ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg taatcaattt
    21061 tcgctttcat cagaacgtcc gatgattggc ttttgcccgg gtgcggagtt tggtccggca
    21121 aaacgctggc cacactacca ctatgcggaa ctggcaaagc agctgattga tgaaggttat
    21181 caggtggttc tgtttggctc tgcgaaagat catgaagcgg gcaatgagat tcttgccgct
    21241 ttgaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca gcttgatcaa
    21301 gcggttatcc tgattgcagc ctgtaaagcc attgtcacta acgattctgg cctgatgcac
    21361 gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccaagtag cccggacttc
    21421 acaccgccgc tatcccataa agcgcgcgtg atccgtctga ttaccggcta tcacaaagtg
    21481 cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac tccccagcgc
    21541 gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat gcgggttttg
    21601 atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact cactgatgcc
    21661 cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt cgcacagatt
    21721 ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg ctggcgtaaa
    21781 gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc gctacaagca
    21841 gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc actggtgaca
    21901 cgtctggcgc atggcgtaaa gcatggattg gactggcaaa ccgctcgcga acctttagcc
    21961 agcctgtttt acaattgtaa gcatcatatt gcaaaacagc agcacgccgt agaacgcacc
    22021 cgcgaactgt ttgccaaaag tttgggctat agcaaaccgc aaacccaggg cgattatgct
    22081 atcgcacagc attttctgac gaacctgcct acagatgctg gcgaatatgc cgtatttctt
    22141 catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga attgattggt
    22201 ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca tgaggaagaa
    22261 cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa gatgagtctg
    22321 gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga tacggggtta
    22381 agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc aaccgatccg
    22441 ggattaattg gtgggtatgg gaagaatcaa gtagagtgtc gttctacaag tatgtctctt
    22501 gcagatttgc cagctcaaac cgtttttcaa aatttaaatc ttgaaataat aaccaataag
    22561 ttgacatcgg agataagatg acctcaacat tatttttctc tctcgagaaa aaaaactgga
    22621 tagcgtactg gaacagagct ctcgtattct tattcattac cacctatttt ttgggtggga
    22681 ttacaaggta taaacatctt attgttattc ttatgacaat aacgacaatc gtctatctct
    22741 gcaaacggcc aaaacactat ctctcactat ttaaaacatt tctttttggt agtgttgcca
    22801 tattaactat tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt gctagcatga
    22861 aggaaatttt caaagctatt attgagaata ctttactatg cacaatagca ataccggtca
    22921 tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca tttattagtg
    22981 cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat caacaaggga
    23041 taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc tttttattcc
    23101 ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct tttgtggttc
    23161 taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc tggctttccg
    23221 tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta ctattagtag
    23281 gagtaatggt ttccatcatt gcattgtcgg ttattttcac acataaggag atgaccgcaa
    23341 agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat ggtactcaag
    23401 gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac ggtaacgttg
    23461 catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc tttagacaat
    23521 caatagggcc acataatttt gcgctattca tctggtttgg cactggttta ttagggctgg
    23581 taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat ggcgtcaaga
    23641 ataaatatcg ctcaccatat aatgcatatt atataatctt actatctttt ataggttatt
    23701 ttgttatccg tggaaacgta gaacaaattg aaccaaattt attaggcgtt tacgccggct
    23761 tattattagc gatgaaaaac aagtaaaaaa taaaaaaggc tgcataatgc ggccttttta
    23821 tttattcaaa ccaattatga ataacctctt cgaatctttg agttacgcca tcccaactgt
    23881 agtgatcaaa cacaaagtcc tgaccttgct ttgcaactgc tgttaattca ggattcgcta
    23941 acgtttttaa aatatcgctg cttatggagt ctgcagtcat aggttctttt aaatggaatc
    24001 ccgtagtatt ttctttgaca aattccgtca tccccccacg cgtactcact aaaacaggct
    24061 taccagcacc catagcttca attgccacca tgcaaaatgg ttcctggaac tgggaaggaa
    24121 tgacgactaa atctgccagt gggtaataac aatgcatctt ttcaggcggt atgcttccta
    24181 acataatgca acgatccttc aaacgttttg caatttccct gacattgcgt tgatatgcac
    24241 ctttgtcact tttactcatt tctgtataat caccaatcac aactaatttt aaatttttgt
    24301 gagcagcggc caatttttcg aatgattgca tcaacagcaa tatgcctttg tcagggacga
    24361 tacgcccagc ataaaaaata attttctctt caggggagat attaagttcg cttctagtga
    24421 tcggctgaaa attagattga taggtttcta aatcaattcc atttggtaca atctcaatat
    24481 ctgcatttgc taaataagat tggtaatact ttttcaagta taaactaggg actatcattt
    24541 taacattttg ctcaagcaat tttggttcaa atgcattatg catatgtata accactcttg
    24601 cctgaggagc tcgcttacga atttgagtgt atagcttcat gctattatga actacaataa
    24661 cactttcttt cgttatagga aaatcatgtg caatattcaa tattctctgt gaatatggaa
    24721 gaggatcaag tcttgtccat ttttgaaata accgcttata tatgcgactg aatccaaccc
    24781 tatgaatact acaattatca ttaacaaagg tgtaatttga gtatcctgga tttttgatac
    24841 aaacaatacg atttggaaaa ttggtacgct gagcaacctg atacatccac gtttcaacag
    24901 cagccgctcc gcgaggagga attgaaaaaa taggagtaac cgtaaatatt attttatcaa
    24961 ccatctcgta tattaacctt tcataacatt atatttaatt gctgtaaaaa caccatcgag
    25021 aaattttttc tggtaaagca agtgtttata tttttctttg tgctcatgtt ttttaacagc
    25081 ttttttataa ggtatatttc tccatggtga tatattataa atattacgaa aataatctgc
    25141 agaggcgtag ttcgcccaat catgccatgg cttagttata ccagtataat gtatgaagac
    25201 agtgtcatca ttaataaacc gggtgtaata ttcactattt ttttcttcaa attctgactt
    25261 tatagtataa atacaattat attttctcgg taatatttta gcatgatgta gaaacattaa
    25321 gtttaaaata tcttgatccg ggtactttaa tttttttata attgactcat ctgataatag
    25381 atcaaagaat ttttccgtta gtctttgttt taaccattcc cgtaaattaa tatacattac
    25441 accagagtta aaatagctac cgttaaaatc ctcattacac aaacgctcag cgcttttact
    25501 ttgcatagca tctacatcaa ttaccactgc cccatattca tctttaaact ctaatgctat
    25561 taactcgttc aatgaaccct tacagacgat atcagcatca agatataata atgaatccaa
    25621 tcgcgcagaa aaataatcaa atgaaagcaa acgataataa atagatactg gccagatatt
    25681 tgattgaggt agcgccttca atggctcaga atcaatatga tataatttaa ttactgtttg
    25741 atacttcgtt gctaattgtg ataataattc aatatactct ttgtctaagt aatcagcgat
    25801 tatgtgaaag gtaaatttgt tttgtttatt cttttcaaga attgaagcaa ttgaaatcgc
    25861 agccccaacc tggtagttct catcaatacc ccatgagaca ttaaacgttt cccgttcatc
    25921 aagtttaacg gggcgcttgt ccagttcaat tatatcttta aattgagtaa gatgtttaaa
    25981 atccaattta attacccttc atcataatca ttatttgctc ttcaatccta gtttgacttt
    26041 tacatccctg attacttttt taattttctt tttgaatata agatatgtat agccaaagtc
    26101 tttcaattca ttttttatat tatagtgccg ttcaagatca atgcgatctt ttgcttttaa
    26161 aacagcggtt gtttttttac ctgaaaggtc aattagtctt aaaccttttt cggatactat
    26221 aaaatttcct ttatgaggat cgccagacac cattccatgc tgatgcaact cttttatcga
    26281 ttctgataat tgatccttca gatcttcaga aatttccaaa tactcattta acccgacacc
    26341 ttcaatatac tcaatgagca taatataata atgagcgaaa tttaatgttt tacgttcagc
    26401 tagcaaaaaa tagtcattaa ttgattggat gccttcacca cgtacccgat cggtttgata
    26461 aattaaattt tcataataat ctttttttat gcaagatttt aagaaacgtt cggtcatttt
    26521 atgctttggc gcataaactt taagtacaag aggtccacga gcagtgtcaa taagaatgac
    26581 tttagtatca tcaatagaac gaaaaacttt taatgtttta accctgcaag ttaaaaagtc
    26641 atttagaact tgctcataaa agggatcatt atcttttatg aaaactttca acccatttat
    26701 cgttttatta tatatcataa tgaattattt taaccttaaa tctttagaag catttttctt
    26761 tataatactt taaataatta aaaatgcctg ccatataatg cttttgttta aatttatgtt
    26821 ttgcacaata ccgatactga ttgctattta caggtttaag taaatcttct ttactccacg
    26881 gagaagctgc tttggcgatc aaaaaactac gtgaaaccgg ataatcagcc cactcatgcc
    26941 agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact ggattatcaa
    27001 ctttgtcttt taattcatag ttaatactat atcgggtatt atatttctcc gaaataaact
    27061 tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg attttactta
    27121 cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt agattccact
    27181 catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaac tgtggtgtag
    27241 ttaataccga ggctcgattt tgccaccatt caatatctct ttcagctaca actgcggcaa
    27301 tttcattagt agaaaattga taatctaaga gttctttaat actacccttg caagcaatat
    27361 ctgcatcaag atatagtatt ttttcatgtt tatgatagaa ataatctgca attataaatc
    27421 gaaaatatgt tgcgtaagtc cagtttttcg tgcttggtaa tgacttcagc ttatcacaat
    27481 tgataacata aatattaatt cttgaattat attgttttgc taaatcagaa aaatataatt
    27541 tgtctttatc actgatataa tctgtgaaaa catgaaattc acaagagatt tctctattgt
    27601 ttaatagaat agatgcgatg gctacaccac atccaaaaag aaaatttctg tcaataccaa
    27661 atgctatatc aagagtattt ttagatctta tattttgata atgaaaatta tactcaaaaa
    27721 ggatgatgtc actatcattg agttgagaca tatttattct gtatccaaaa ttttggcaaa
    27781 aataagttac gggttacaac gattttcgaa tcgttctttc cctgattttt gtggcttttg
    27841 cttctgcttg cgacagcagt ccttgttcct gcttgagaat gtctttaagc ggggcggcaa
    27901 aatacacctt cataaagcgc cagatatccc gctgagtcag gccaatattc attgaagaaa
    27961 aataaagccc tataagatct ttatcgcgcc aacgacctgg aacgcgcgtg cgaagctgcg
    28021 cccggtgtag gtcaattacc gaaattttta actcctcttc cttaccggaa aaaggcaagt
    28081 gcagcaggaa atgacagata taacagtcac gatggttaat gcccgcagca tgcatatcgc
    28141 gcaccatcgt cgcgacacgc ttaataagca tacgctttac gcgaacatct ggtgggttgg
    28201 tcgcccagtc agcacagtaa tcttccagac tgatggttgg tgtcagatct tcggtaataa
    28261 taaacgaggt tctggtcagc ggattcatgc ctttttcgcc gaatgccacc ccatacatag
    28321 tatcaacgcc gacatcccgc agtcgatgaa tcgcattcca ttcgcggtct gcgcctaata
    28381 ctggcatccg caatgagagt aaatttttga ttatctcttt cagggtcgtg ccgcgatgcc
    28441 atttgagaaa atagcttttt cccgccattt caaagcgcag agtacggcga gtttccagtt
    28501 cacgaaatac ctcaccctgc aaggttttaa cttcctcaaa aggatctttg ccgcgccata
    28561 acgtggcaaa cggctcttta agttcaacca tccagaccac ccgttatgat atccgccgct
    28621 ttctctggca gactgtataa atcttgtgta tcagcataat gtcgcgcatt ttccgcccaa
    28681 gcctggcgca atgaagattg cgttaacgct ttgcgtaaaa tctcattcaa tgtttcctgg
    28741 cggaatggct cagcaatagc ctcgccgcaa ttagcgtcga caatataatg cgcatagcca
    28801 caaacggcag ttgttagtac cggtaatcct gcagtaatcg cttccagcag cacaattccc
    28861 gccgcttcct ggtaggcagg atgcagtaat aaatccgccg ccgccattaa ttccgagaca
    28921 tcgttgcgcc ccgagaagaa gtgaacatta ctgcgcacgc cgcgtttttc tgccagtgcc
    28981 tcaaattttc gcggtttatc ctgcccaaca acatatagca atgtgttgtg gcgcagcgaa
    29041 tccggtaacg aagcaagtgc ttcaatggaa cgatcgacac ctttacgcgt gaagtctgaa
    29101 ccgacctgca acaataaata ttgttgttcg gttattccat tcttcttacg gaagatttca
    29161 cggctattgg ctggctgctg gctatattta cgatcaggat aaatccccgg tggcagaata
    29221 tgaaaacgct ccgcttcagt ctgataatgt ttctggaaat cggcgatttg cttatctgtc
    29281 agcatcagca gctgtgtcgg cttgccctgt tcgaaggttg cccgctcaaa ggcggcataa
    29341 tggcgataac gtgacgtcag gcgatagaaa aagccttttt cctgcgctac tttctcggca
    29401 taacaaacat cagcggcata ataaacgtcc agccccggca ttttgttgaa tccaacgact
    29461 ttatcgacgg gatgttcgcg taaatgtttt tgcacccagg caaaatactc cgcattgcgc
    29521 ccgtgattgg tatgcgattt aaccggcact ttgatcagtt caaatacatc agggcattcg
    29581 ccttcccacg actgggtata aacccgaaca tgatgacctc gggcggcgac tgtctgagca
    29641 atacgcataa aatcgcgctg caaaccgcca aagggaaaat atttatataa acaaaaagca
    29701 acgatcataa cgacgtacct gtcgtggagg agggcagtaa tttatcgaca gcagcaatga
    29761 catctgccgc cggaataacc gaaagataca tctcatttcg gtcacgctga tcgcgcgttg
    29821 gcatttcccg gtaatctccc gcccagaatt gaatcatgtt atttgaccag ggacgccaga
    29881 aaatatgatc tgttgcgcca aacagcgata tcagcggcgt attaactgca gcggcaatat
    29941 gcgccggtgc ggaatcaacg ccaataaaca gctgcgcatg atcgattaac gcaccaagtt
    30001 ccgggaaggt cacctttcca gccagcgccg ttactggtgg cgtctggcat ccctgcgcaa
    30061 tttcattgac gcaggccaga tcgtctttat ctgggccgga cgtcagaacg acttcataac
    30121 cacgagcatg taaggcatca atcacagcgg aaaacttggc gttgtcccag catttgaaga
    30181 tttgccgcgc cgtaggttgg ataaccacat aattttgtcc aacaccagcg tgatcaagtt
    30241 cgcgacgcat acgtttccag cttgcaggcg ggtaactcat ggttgtctgc ttcaccaacg
    30301 aatcaagtcc caatggtgtc agcacggata agttactttc caccacattt ccaccctgca
    30361 acggcaccaa atgggtgaaa cttttacgcc aaaaagcaga ctgccgatga tgataatcct
    30421 gggaaatttt cacacgagca tttaataagc gaaccagtat agcaaccatc cattgatcgg
    30481 taagattgac gataaggtca tacttattgg cacgtaatac cttgatgaga tgaaaaaagt
    30541 tggcaatttt ttctgaggct tttgcttttt tattttttat gccgtagagc gcgttaatct
    30601 ctggattttc agacaggatc gggatggtgt cctgataaag cagcacatcg atttttgcgt
    30661 cagggtaatt ttttttcaga gaactaatga cgggagtagt taataacata tccccatgaa
    30721 aacgcatctt aatgagcaaa attcttcgaa atggcttatc cacaagtaac tctttttgtt
    30781 aagattatcc gattaaggtg aagcagaaaa agcacgctac cgccccaggc tcaacagcta
    30841 cctgaatact gataccgcac tgatattgac gcgctagtgt atcacttctt tcgcggcaat
    30901 ccgacccgaa tgatttttaa gcacattgtt gattttcaat aaagaaatca atcaaataaa
    30961 atcattagca accatattct ttcactgttg ctctgcgatt ccttcctgca cgtacagcag
    31021 aaatcaatga atcgcataaa aatacaaaaa aagtatagat gttcagcaag aattggcggg
    31081 taaattgcta tttaa
//
LOCUS       DADLTI010000032        30204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLTI010000032 DADLTI010000000
VERSION     DADLTI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.032"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..210
                     /locus_tag="JN755_001316"
     CDS             <1..210
                     /locus_tag="JN755_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7380170.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="JN755_001317"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="JN755_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7380171.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="JN755_001318"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="JN755_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7380172.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="JN755_001319"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="JN755_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7380173.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="JN755_001320"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="JN755_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7380174.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="JN755_001321"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="JN755_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7380175.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="JN755_001322"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="JN755_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7380176.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="JN755_001323"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="JN755_001323"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7380177.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="JN755_001324"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="JN755_001324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7380178.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="JN755_001325"
     CDS             complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="JN755_001325"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7380179.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14027..14872)
                     /gene="thiG"
                     /locus_tag="JN755_001326"
     CDS             complement(14027..14872)
                     /gene="thiG"
                     /locus_tag="JN755_001326"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7380180.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="JN755_001327"
     CDS             complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="JN755_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7380181.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="JN755_001328"
     CDS             complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="JN755_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7380182.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="JN755_001329"
     CDS             complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="JN755_001329"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7380183.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="JN755_001330"
     CDS             complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="JN755_001330"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7380184.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18342..18442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="JN755_001331"
     CDS             complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="JN755_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7380185.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19066..19839
                     /gene="nudC"
                     /locus_tag="JN755_001332"
     CDS             19066..19839
                     /gene="nudC"
                     /locus_tag="JN755_001332"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7380186.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19879..20943
                     /gene="hemE"
                     /locus_tag="JN755_001333"
     CDS             19879..20943
                     /gene="hemE"
                     /locus_tag="JN755_001333"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7380187.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21100..21771
                     /gene="nfi"
                     /locus_tag="JN755_001334"
     CDS             21100..21771
                     /gene="nfi"
                     /locus_tag="JN755_001334"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7380188.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21814..22404
                     /locus_tag="JN755_001335"
     CDS             21814..22404
                     /locus_tag="JN755_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7380189.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22591..22863
                     /gene="hupA"
                     /locus_tag="JN755_001336"
     CDS             22591..22863
                     /gene="hupA"
                     /locus_tag="JN755_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7380190.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22876..23571
                     /locus_tag="JN755_001337"
     CDS             22876..23571
                     /locus_tag="JN755_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7380191.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23573..23998)
                     /gene="zraP"
                     /locus_tag="JN755_001338"
     CDS             complement(23573..23998)
                     /gene="zraP"
                     /locus_tag="JN755_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7380192.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24236..25612
                     /gene="zraS"
                     /locus_tag="JN755_001339"
     CDS             24236..25612
                     /gene="zraS"
                     /locus_tag="JN755_001339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7380193.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25609..26934
                     /gene="zraR"
                     /locus_tag="JN755_001340"
     CDS             25609..26934
                     /gene="zraR"
                     /locus_tag="JN755_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7380194.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26931..28220)
                     /gene="purD"
                     /locus_tag="JN755_001341"
     CDS             complement(26931..28220)
                     /gene="purD"
                     /locus_tag="JN755_001341"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7380195.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28232..29821)
                     /gene="purH"
                     /locus_tag="JN755_001342"
     CDS             complement(28232..29821)
                     /gene="purH"
                     /locus_tag="JN755_001342"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7380196.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7067 a         7874 c         8314 g         6949 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gcggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tgcgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacttg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagaccccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacacc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5341 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 attctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga aggaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga actggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct gggtccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgacagacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgaaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ctgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaactaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
     9961 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg catcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcaaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac
    11581 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cttggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc
    11881 gaaagaaccg gcaatcttgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtaa agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 tgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg
    12721 gcaaattctt cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc
    12781 cctggacaac attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct
    12841 tgcgtttttc atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat
    12901 agtctttgcg aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc
    12961 tgagcggtta acgcggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt
    13021 tccaactcgg ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg
    13081 ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt
    13141 gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt
    13201 tcatccataa tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag
    13261 acagagtaac ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata
    13321 tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca
    13381 atccccgcac gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg
    13441 cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc
    13501 ccatccagac caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc
    13561 atttgtagtg aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc
    13621 cccactttcg cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt
    13681 atagcggcgc tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta
    13741 ctcatggaaa atccgcagta cgtgcagttg ttagcgcaaa gattggaaag ataaagcggg
    13801 acgtagaaac taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ctgttgggcc
    13861 agttgttcca gatagccact ggcggcaggc gataacagcg ccatcatgtc gtcgcgggtg
    13921 agttgcgagg catttagcgc ccgctctacg tcagcagccg ttttgccgtt gatacgcagg
    13981 cggatgtcgt cccagtccag ttgtcgccag cgattgctga aggttttcat gccgatgcct
    14041 ccagaaatcc ggtcagcggg ctggtggcat gagcaaaata actgcggctg cccggtccgg
    14101 actgacgtgc cagcaggcct gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg
    14161 gatcgtccgc gacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccagcg
    14221 cctgcgcggc atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct
    14281 gctggataat aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg
    14341 cgccgagcgg catcaccgct gcacagccga cttcttccag acgtttacac aataccggat
    14401 cggccccgca gtaaggcagc acgacaaatc cctgttgtac cagcgtttcg gcggctttca
    14461 gggtttcgat gggatcgggc aacagccagc gggcgtcagg gtgaatctcc aattttagcc
    14521 agtttgtgcc taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt
    14581 tcgccccgga agtattcggc agcagggcca cacccgccgc gataagcggt tcgaggatag
    14641 cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg
    14701 aagcgcggat cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca
    14761 gatgtgaatc aaacgttttg tccacaatac gtaacatctc aaccccctgc aataacctga
    14821 aaaagcagga tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg
    14881 atttgctgat taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt
    14941 tcgtgaacag tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt
    15001 gcttcctccg cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct
    15061 cgacttaccg tcgaacagtc ggagttctcc cgcaggcgtc tctataccgc ttaataactt
    15121 aatggcttcc agtgcctgca aagtgcccat caccccgacc accgggccaa ccacgcccgc
    15181 cgtgcggcag ttgcgttctg gctgctggtt atctggccac aggcagcggt aacacccctg
    15241 ctcccagggc ggtgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat
    15301 aagcggcgtg ttgagtgcca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc
    15361 ggtacagtcg agcaccacat cggcttgtgc aaccgcatct ttcaacgcct cacccgttaa
    15421 ccgttgttgt aatgctgtca gttgaatgtc gggattcaac tgtgtcagtc gctgttggct
    15481 gacctgcgat ttcgggctgt tgatatctgc agtggtaaag aggatttgtc gttgcagatt
    15541 gcttaaatgc acatcgtcgt catctgccag taccagcgtc ccgacgccag cccctgccag
    15601 gtatagcgca gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag
    15661 cagtttttgc tgcccgtcaa gagcgatatc gtcgagcaga atttggcggc tataacgcat
    15721 aaagtcacgg tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac
    15781 gccagtctgc ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta
    15841 tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc cacggtggga taatccgcca
    15901 ggcgctcaac atgccgtgcc aactgttcca gcccctgcgg tgcagaaggc atctgtttgg
    15961 tttgcgtcgg gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt
    16021 cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat
    16081 tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga
    16141 tcgcgagccg ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa
    16201 ttgccgccac gacatcggct tccacctctt catcgcgccg atctttgatg cgtagctgga
    16261 gagtacgtac gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt
    16321 acagtcctaa acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt
    16381 atgcaggtag atttctccgc ctctggcacg gaagttctcc gacatatccg ccattcccac
    16441 ttcaatggtt tgcgcggcgg cgtaatcgcg tacttcctgg ctgattttca tcgagcagaa
    16501 ttttggccca cacatggagc aaaaatgggc gactttgccg gactcttggg gcagggtttc
    16561 atcgtgataa gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg
    16621 aaattcgaag cgggctttcg acatggcgtt atcgcgaatt tgcgcgcccg gatgcccttt
    16681 tgccaggtca gcagcgtggg cggcaatctt ataagtgata agcccctgct taacatcttc
    16741 tttattgggc agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa
    16801 ccagccaatc atcgccgcac caatccccga cgtgaagtgg tcatagcccg gtgcaatatc
    16861 ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt
    16921 catattgcgg tggatcatct gcatcggcac atggcctggg ccttcaatca tcacctgtac
    16981 gtcatattcc caggcgattt tggtcagttc gcccagcgta tgcaattcgg caaactgcgc
    17041 ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac
    17101 gtcataagcg gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg
    17161 atgatgggag aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag
    17221 gcgtttcgcg gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc
    17281 cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc
    17341 ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg
    17401 actgttacgc aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat
    17461 caccgtatcc gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga
    17521 agaggtgacc gccgagttgc cgatattggc gttaactttt accaggaagt tacggccaat
    17581 aatcatcggc tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc
    17641 aacttcatca cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat
    17701 tcccggatgc tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg
    17761 gatggcgatg aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt
    17821 gacacggcgt cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc
    17881 gagaccatca tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc
    17941 ggtatcggcg cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt
    18001 aatggcgatc tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt
    18061 ttcttcgtac tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc
    18121 acgcatcggc acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg
    18181 aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg
    18241 ggtcagtttt gttgcagaca tagctcattc caaaaagtta aggacgtggc ttgtcagacg
    18301 acggatgaag caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc
    18361 ccttcgcagg tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt
    18421 taaggcactc cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt
    18481 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    18541 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    18601 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc
    18661 ataatctgtt gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    18721 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    18781 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    18841 tctttgccag gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    18901 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    18961 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19021 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19081 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19141 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19201 atcggcgaat ggcaggggga acctgtttgg ttaatacaac agcagcggcg tcacgatatg
    19261 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgtggc
    19321 gtgcaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    19381 tatccgagca aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg
    19441 caaatcgccc cctgcattat tgttgccatc cgtcgcgatg attcgatcct cctcgcccag
    19501 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    19561 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    19621 ttgcgttacg tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg
    19681 gcggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    19741 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    19801 gaagatactg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    19861 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    19921 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    19981 gaatataaag ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag
    20041 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc
    20101 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20161 gaaggtccgc gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt
    20221 ccggacccgg aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcacgaa
    20281 ctgaaaggcg aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcaacctac
    20341 atggtggaag gcggcagcag caaagccttc accgtgatca aaaaaatgat gtatgccgat
    20401 ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    20461 gcgcagatta aagccggtgc tcaggcagtg atgattttcg acacctgggg cggcgtgctt
    20521 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    20581 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    20641 tggctggaag ccatggctga aaccggttgc gatgcgctgg gcctcgactg gacaacggac
    20701 atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg
    20761 tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt
    20821 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    20881 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc
    20941 taagagcctg atccatcagg ctattttatt tgccattttg aggccgggca gtgctcaaaa
    21001 tcctcacgta ctacgtgtac gctccggttt ttccgcgctg tccggcctca aactgccttc
    21061 aacaattacg cctgttgaac caggctctaa ggagcgatta tggatctcgc gtcattacgc
    21121 gctcaacaaa tcgaactggc ttcttctgtg atctgcgagg atcgactcga taaagatcca
    21181 ccggatctga tcgccggagc cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg
    21241 gcgatggtgc tgctgaaata tccctcgctt gagctggtgg agtataaagt tgcccgcatc
    21301 gccaccacca tgccttacat tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg
    21361 gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc
    21421 tcgcatcctc gccgtcttgg cgtcgccagc cattttggct tattggtgga tgtgccgacc
    21481 attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac cgctctccag caaaccgggc
    21541 gcgctggccc cactgatgga taaaggcgag cagctggcct gggtctggcg cagcaaagcg
    21601 cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag cgcgctggcg
    21661 tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg ggcggacgcg
    21721 gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta attcaggtaa
    21781 actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat tcatctgcgt
    21841 ctggagcgcc tggaaagctg gcagcatgtc actttcatgg cttgcttatg cgaacgcatg
    21901 taccccaatt acgccatgtt ctgccagcaa accggttttg gtgatgggca aatttaccgt
    21961 cgtattctcg atctcatctg ggaaacgctg accgttaaag acgcaaaagt aaatttcgac
    22021 agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga tctgtacggc
    22081 gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt
    22141 ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac cgttgcgatg
    22201 ctggaaatga ctcaggctgg tcgcgaaatg agcgatgaag agcttaaaga aaacccagct
    22261 gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga gtgcgaagaa
    22321 cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgagtcggg tgagagcaat
    22381 attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg atttgtcgta
    22441 cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa aaaaagtggc
    22501 tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact cgatgcttag
    22561 caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat tgatgtaatt
    22621 gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc cactctggct
    22681 gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt cggtaccttc
    22741 aaagtgaacc atcgcgctga gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa
    22801 attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga cgcagttaag
    22861 taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct tttgtctttc
    22921 tggcgtcgat cattgatgct ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct
    22981 tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt acgcatctgg
    23041 cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc
    23101 gataccacga cgcgagagta tcgctggcag ggcgataacc tcacgctcat caatatcaat
    23161 gtttacagca aaccgccggt gaacattcgc gcgagttttg acgatcgcgg cgatctgagc
    23221 tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca aatcgattta
    23281 taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca ggggagagtc
    23341 gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa
    23401 accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc
    23461 tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca attactgtta
    23521 gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg atttaccagt
    23581 ggcccatacc catatgaccg ccaccaccgc agccgccgta gcccatgccc attccggctc
    23641 cgcgcggaat acccgcttca gccatcgcga tatctcgttt cacccgtaac tcatctaacg
    23701 actgacgcaa attctccatc tctttggcga ccgcgttaat tttgctgcta tccggtgggt
    23761 tcgcggctaa cagggcattg tattcataac gcttcgtcac cagttgctgt tgcagtgcgc
    23821 tgctttgagc gtaaaagtca ttatggattt tctgccacgc tgtctgttgt tcgctggtca
    23881 aaggcgcggc attttgctgc cacataccgt gtccgccgtg agcaaatgca gatgtcgatc
    23941 ccatcgccat tgctgaaagc gccatcatta ccagggcaat tttcgtgttc cgtttcatgg
    24001 ttaatcctcc agtggttgtc ttacttcggg tattgcatct tccgtgccaa cgatgaaacg
    24061 ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta
    24121 gcgagtcatt tttactcatt gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg
    24181 ataaaagatg gcatgatttc tgctgtcaga aagggatgag caggcaaaga agaagatgcg
    24241 ttttatgcaa cgttctaaag actccttagc taaatggtta agcgcgatcc tccccgtggt
    24301 cattgttggg ctggtagggt tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac
    24361 tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc
    24421 cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg cagcagcagg ccttactgga
    24481 agaaatggcc gggcagcctg gagtacgttg gtttgcggtc acggatgaac aaggaacaat
    24541 cgtgatgcat agcaactccg gcatggtggg aaaacagctt tattcccctc aggaaatgca
    24601 gcagttacat ccgggagatg aagaagcgtg gcggcggatc gatagcgcag acggcgagcc
    24661 tgttctggaa atttatcgcc agtttcaacc gatgtttgct gctggaatgc accggatgcg
    24721 ccatatgcag cagtatgccg cgacaccaca agcaattttc atcgctttcg acgccagtaa
    24781 tattgtgagt gccgaagatc gtgagcagag aaacaccctg attattctct tcgccctggc
    24841 gacggtcttg ctggcaagcg tattgtcatt cttctggtat cgccgctatc tgcgctcgcg
    24901 ccagcttcta caagatgaaa tgaagcgcaa agagaagctg gtggcgctgg ggcatcttgc
    24961 ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg attaaaggac tggcgaaata
    25021 ctttgccgag cgcgcgcctg cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa
    25081 agaggccgac cgtttaaacc gcgtggtaag cgagttgctg gaactggtta agccaacgca
    25141 tctggctttg caggcggtgg atctcaacac gctgattaac cactcattac agctggtaag
    25201 ccaggatgca aacagccggg agatccagtt acgctttacc gccaacgaca cattaccgga
    25261 aattcaggcc gatccggaca ggctgactca ggtcctgttg aatctctatc tcaatgctat
    25321 tcaggcgatt ggtcagcatg gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt
    25381 gaaaatcagc gttaccgaca gcggtaaggg aattgcggca gatcagcttg aagccatctt
    25441 cactccgtac ttcaccacca aagccgaagg caccggattg gggctggcgg tcgtgcataa
    25501 tattgttgaa caacacggtg gtacaattca ggtcgcaagc caggagggaa aaggctcaac
    25561 gttcaccctc tggcttccgg tcaatattac gcgtaaggac ccacaaggat gacgcacgat
    25621 aatatcgata ttctggtggt ggatgatgac attagccact gcactatttt gcaggcttta
    25681 ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag
    25741 gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc
    25801 atcgccacgc tgaaagagat caaagcgtta aacccggcaa ttccggtgct gattatgact
    25861 gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa ctggggcgct ggattatctc
    25921 atcaagccgc tggatttcga taacctacag gcgacgctgg aaaaggcgct cgcgcatacg
    25981 cacagtattg atgctgaaac acctgcggtg actgccagcc agttcggtat ggtcggtaaa
    26041 agcccggcga tgcaacacct gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg
    26101 gtgctgatcc acggcgattc cggcaccggt aaagagctgg tcgccagggc gattcacgcc
    26161 agtagcgcac gtagcgaaaa accgctggta acgctcaact gtgcggcact caacgaatcc
    26221 ttgctggaat ctgaattgtt cggtcacgaa aaaggggcgt ttactggagc cgataaacgc
    26281 cgggaagggc gctttgttga ggcggacggc ggcacgctgt ttctcgatga aattggcgat
    26341 atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt
    26401 gtcggtagca accagactat ctcggttgat gtccggctga ttgcggcgac tcatcgcgat
    26461 cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc tctactatcg cctgaatgtg
    26521 gtggcgattg aagtaccatc gctgcggcaa cggcgggaag atatccctct gctggctggc
    26581 cattttcttc agcgctttgc cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag
    26641 gcgatggatt tgctgatcca ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg
    26701 gtggaacggg cggtggtgct gctgaccggg gaatatattt ccgaacgcga gctgccgctg
    26761 gcgattgcca gtacgccgat cccgctgggg caaagtcagg atattcagcc gctggtggaa
    26821 gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc
    26881 gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc
    26941 tcgcgttcga tggcgcgcca accgatatct ttccggcaga agcaatcatc ccagtggata
    27001 tcggtcatta aggcgtaggc gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg
    27061 acgcacagta cgcgcccgcc gctggttacc acctgctcgt catccgccag ttttgtgccc
    27121 gcgtggaaca ctttgccgtc agccacttct tccagcggca ggccgtggat cacatcgccg
    27181 gtgcggtaat cgcccggata tccacccgca gccatcacca cgctgagaga agcacgttca
    27241 tcccactctg acgttttctc gtccagtttg ccttcacagg ccgccaggca gagctcaacc
    27301 agatcggact tcatgcgcag cataatcggc tgggtttccg gatcgccaaa gcggcagtta
    27361 aattcaataa ccttcggatt gccctgtttg tcgatcatca ggcccgcata gagaaaaccg
    27421 gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg gccagatgat acgttccatg
    27481 gtgcgctgat gaacttcatc ggttactacc ggcgcggggg agtaagcgcc catcccgccg
    27541 gtgtttggtc cgttatcttt atcgcctacg cgtttgtgat cctggctggt agccatcggc
    27601 agcacatgct cgccgtccac catcacgata aagctcgctt cttcgccatc gaggaactct
    27661 tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca
    27721 gccgcttccg cttcttccag cgtcatcgcc acgataacgc ctttcccggc agccagaccg
    27781 tccgctttaa tgacgattgg cgcgcctttc tcacgcagat acaccagcgc aggttctacc
    27841 tcggtgaagt tctggtattc cgccgtaggg atcttatggc gggccaggaa atctttggta
    27901 aacgcttttg agccttccag ttgggccgca cctgcggttg ggccgaagat tttcagcccg
    27961 gcggcgcgga aggtatcgac cacgcctttc accagcggcg cttccgggcc gacgatggtc
    28021 agatcaatct tttcgttttg tgcgaaatcc aacagcgccg ggatatcggt cacgccgata
    28081 gcaacgtttt gcagcgcggg ttccagcgca gtgcctgcat tacccggagc aacaaaaaca
    28141 gtctcaacca gcggcgactg ggccgctttc caggccagcg cgtgctcgcg cccgccgtta
    28201 ccaatcacta atactttcat ctattgctcc attaatggcg gaagtggcgc atgtcggtga
    28261 agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac
    28321 cgccaggctg gattacgcag gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga
    28381 acgggaagaa cgcgtcagaa gccatcgagg aacctttcac ttccaggcct tcatcggcag
    28441 ctttaatacc ggcgattttc gcggagtaca cgcggctcat ctggcccgcg ccaataccga
    28501 tggtcatatt gtttttggca tagacgatag cattggattt cacgaatttc gccactttcc
    28561 agcagaacag cgcatcacgc agttcctgtt cggtcggctg acgttgggtg acgacgcgca
    28621 gttcttccgc accgaccatc cccaggtcac gatcctgaac cagcagaccg ccgttcacgc
    28681 gtttgaaatc aagacccgga acacgctcgc cccactgacc gcaggtcaga acgcgtacgt
    28741 tctgcttggc ggcggtgatt ttcagggctt cttcgctggc ggaaggcgca ataatcactt
    28801 cgacaaactg acgagaaatg atggcctgtg cggtttccgc atccagctcg cggttaaagg
    28861 caatgatgcc gccgaatgcg gaggttgggt cggttttgta cgcgcgatcg taagcatcaa
    28921 gaatggaatt gccgatagct acaccgcaag ggttggcgtg cttcacaatc acacatgccg
    28981 gctcggcgaa ctctttcacg cactccagcg ccgcatcggt atcggcgatg ttgttataag
    29041 agagggcttt accctgaacc tgggttgcgg tagcaacgga ggcttctttc acattctctt
    29101 ctatatagaa ggcagcctgc tggtggctgt tctcgccgta acgcatatcc tgcttcttaa
    29161 tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt
    29221 acgccggaac catgctgccg aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt
    29281 cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat
    29341 ccatctcttt aataatggcg tcgtagtcgc tgctcttcac cacgattgcg acatctttat
    29401 ggttcttagc ggcggagcgc accatggtcg ggccgccgat atcgatgttc tcaaccgcat
    29461 cttccagcga gcagccttca cgagcaacag tctgggcgaa cggatacagg ttaacaacca
    29521 ccatatcgat aggctggatc tgatgttctt ccataatggc atcgtcctgg ccgcgacggc
    29581 ccagaatgcc accatgtact ttcggatgca gggtcttcac gcgtccatcc atcatctccg
    29641 ggaaaccggt gtaatcggaa acttcggtta ccggcagacc tttttctgct aacagacggg
    29701 cagtgccccc tgtagacagc agctccacac cgcgtgcgga aagtgcctgg gcgaattcga
    29761 cgataccggc tttgtcagaa acactgagca gagcgcggcg gactggacga cgttgttgca
    29821 tggtaaatcc cttgggtttg actattacag agagcgttag ctgaattttt cgcgaaaaac
    29881 tcagctaacg cccctaacgg ggcatcctta tttttcgccc gcattgtaac gaaaacgttt
    29941 gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac aattttgaca gtggttaccg
    30001 tgggaaaaat acagaaatta cattgatgat tgtggataac tctgtgcgta aaaaggtata
    30061 aagcggggtt ttgctgggga atgcagcagt cagtcatttt tctgcaattt ttctattgcg
    30121 gcctgcggag aactccctat aatgcgcctt catcgacacg gcggatgtga atcacttcac
    30181 acaaacagcc ggttcggttg aaga
//
LOCUS       DADLTI010000033        29694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLTI010000033 DADLTI010000000
VERSION     DADLTI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.033"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..1280
                     /locus_tag="JN755_001343"
                     /pseudo
     CDS             1..1280
                     /locus_tag="JN755_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            1302..2711
                     /locus_tag="JN755_001344"
     CDS             1302..2711
                     /locus_tag="JN755_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380197.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2689..3468
                     /locus_tag="JN755_001345"
     CDS             2689..3468
                     /locus_tag="JN755_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY7380198.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3432..4325
                     /locus_tag="JN755_001346"
     CDS             3432..4325
                     /locus_tag="JN755_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7380199.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(4473..5048)
                     /locus_tag="JN755_001347"
     CDS             complement(4473..5048)
                     /locus_tag="JN755_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7380200.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5065..5325)
                     /locus_tag="JN755_001348"
     CDS             complement(5065..5325)
                     /locus_tag="JN755_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7380201.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(5316..6587)
                     /locus_tag="JN755_001349"
     CDS             complement(5316..6587)
                     /locus_tag="JN755_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7380202.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6665..7030)
                     /gene="queD"
                     /locus_tag="JN755_001350"
     CDS             complement(6665..7030)
                     /gene="queD"
                     /locus_tag="JN755_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7380203.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            7346..9145
                     /gene="cysJ"
                     /locus_tag="JN755_001351"
     CDS             7346..9145
                     /gene="cysJ"
                     /locus_tag="JN755_001351"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7380204.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9145..10857
                     /gene="cysI"
                     /locus_tag="JN755_001352"
     CDS             9145..10857
                     /gene="cysI"
                     /locus_tag="JN755_001352"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7380205.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10931..11665
                     /gene="cysH"
                     /locus_tag="JN755_001353"
     CDS             10931..11665
                     /gene="cysH"
                     /locus_tag="JN755_001353"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7380206.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            11929..12081
                     /locus_tag="JN755_001354"
     CDS             11929..12081
                     /locus_tag="JN755_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7380207.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            12275..13426
                     /locus_tag="JN755_001355"
     CDS             12275..13426
                     /locus_tag="JN755_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7380208.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAIDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(13512..14549)
                     /gene="iap"
                     /locus_tag="JN755_001356"
     CDS             complement(13512..14549)
                     /gene="iap"
                     /locus_tag="JN755_001356"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7380209.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14801..15709
                     /gene="cysD"
                     /locus_tag="JN755_001357"
     CDS             14801..15709
                     /gene="cysD"
                     /locus_tag="JN755_001357"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7380210.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15711..17138
                     /gene="cysN"
                     /locus_tag="JN755_001358"
     CDS             15711..17138
                     /gene="cysN"
                     /locus_tag="JN755_001358"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7380211.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            17138..17743
                     /gene="cysC"
                     /locus_tag="JN755_001359"
     CDS             17138..17743
                     /gene="cysC"
                     /locus_tag="JN755_001359"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7380212.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17793..18116
                     /locus_tag="JN755_001360"
     CDS             17793..18116
                     /locus_tag="JN755_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7380213.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            18310..18621
                     /gene="ftsB"
                     /locus_tag="JN755_001361"
     CDS             18310..18621
                     /gene="ftsB"
                     /locus_tag="JN755_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7380214.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18640..19350
                     /gene="ispD"
                     /locus_tag="JN755_001362"
     CDS             18640..19350
                     /gene="ispD"
                     /locus_tag="JN755_001362"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7380215.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            19350..19829
                     /gene="ispF"
                     /locus_tag="JN755_001363"
     CDS             19350..19829
                     /gene="ispF"
                     /locus_tag="JN755_001363"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7380216.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19826..20875
                     /gene="truD"
                     /locus_tag="JN755_001364"
     CDS             19826..20875
                     /gene="truD"
                     /locus_tag="JN755_001364"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7380217.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20856..21617
                     /gene="surE"
                     /locus_tag="JN755_001365"
     CDS             20856..21617
                     /gene="surE"
                     /locus_tag="JN755_001365"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7380218.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21611..22237
                     /gene="pcm"
                     /locus_tag="JN755_001366"
     CDS             21611..22237
                     /gene="pcm"
                     /locus_tag="JN755_001366"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7380219.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            22377..23516
                     /gene="nlpD"
                     /locus_tag="JN755_001367"
     CDS             22377..23516
                     /gene="nlpD"
                     /locus_tag="JN755_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7380220.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23579..24571
                     /gene="rpoS"
                     /locus_tag="JN755_001368"
     CDS             23579..24571
                     /gene="rpoS"
                     /locus_tag="JN755_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7380221.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(24691..25098)
                     /locus_tag="JN755_001369"
     CDS             complement(24691..25098)
                     /locus_tag="JN755_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7380222.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            25245..25838
                     /locus_tag="JN755_001370"
     CDS             25245..25838
                     /locus_tag="JN755_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7380223.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25838..26257
                     /locus_tag="JN755_001371"
                     /pseudo
     CDS             25838..26257
                     /locus_tag="JN755_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(26536..27099)
                     /locus_tag="JN755_001372"
     CDS             complement(26536..27099)
                     /locus_tag="JN755_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY7380224.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27252..27503
                     /locus_tag="JN755_001373"
     CDS             27252..27503
                     /locus_tag="JN755_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7380225.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27505..29601
                     /locus_tag="JN755_001374"
     CDS             27505..29601
                     /locus_tag="JN755_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7380226.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7272 a         7343 c         8038 g         7041 t
ORIGIN      
        1 ccacagagta agtatcccgt gtgctgaatt ttatgaatgc gcacaaaatt aacggtgtgc
       61 cgcgtaccgg tgcttccgcc accgaaggtg ggctggaaac tgttgtagaa aactcggtgg
      121 tgctcgacgg ctccgccatg aatcaaatca ttaatattga tattgagaat atgcaggcga
      181 cggcgcaatg tggtgttccg ctggaagtgc tggaaaacgc gttgcgtgaa aaaggttaca
      241 ccacggggca ttctccgcag tcaaagccgc tggcgcagat gggcggcctg gtagcaaccc
      301 gcagtatcgg gcagttctcc acactctacg gcgcaatcga agatatggtc gttggtctgg
      361 aggcagtgtt ggcagatggc accgtcacac gcattaaaaa cgtgccacgc cgcgcggctg
      421 gcccggacat tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag
      481 taacagtgaa aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg
      541 aagacatgaa aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt
      601 cgatcgctcg tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg
      661 gaaaatgggt gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg
      721 aagggattgc ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga
      781 ttgaaacctg gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc
      841 agatcctcaa aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca
      901 tccacgaaat ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg
      961 acatcaccat gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact
     1021 tcgtctatga ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca
     1081 atccgctcaa caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc
     1141 accatggcat tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg
     1201 cgttgctgga agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta
     1261 tctatccgat tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt
     1321 tcggcttctc aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca
     1381 cttcaccggt gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctgcgc
     1441 tcactttcgg cgcacacctg accgacggtt atgttctcgg cgtcattggt tacgccatta
     1501 ttcaacttac gcccgccatg caactgacgc cgtttatggc gggaatgagc ggcggctcgg
     1561 cgctccttgg tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc
     1621 ggcaaaaaat cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt
     1681 ttgccaccac gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag
     1741 gcgattactc agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta
     1801 ttttgctggg cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg
     1861 gacatcactt tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc
     1921 tgcccgcgtt gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac
     1981 tgtgccaggg gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg
     2041 ttttactggg cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt
     2101 cgcgttactg gcggcgcacg gcgtttaaca gcatcttctt tgtctgcctc gtaatcccat
     2161 ggtttgtgat ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc
     2221 tgactgccag cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc
     2281 tgacgcacct gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa
     2341 cgctggtagt aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc
     2401 tcttcagcac caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt
     2461 ttcctactga cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg
     2521 cggcggtaag tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct
     2581 tattgctcct ggcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag
     2641 aaactaaagc cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg
     2701 ttagcgttta aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg
     2761 gctcagggta atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa
     2821 caggccgctg ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc
     2881 gcgttgagca tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg
     2941 tttgaggaag ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc
     3001 gctcgccata ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc
     3061 caaagcagcg aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg
     3121 ccctgcttca ccctggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag
     3181 cgtactgaac atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt
     3241 cagtgcggtg aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa
     3301 gcagaaatta tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg
     3361 gcaagagcag agcaacgatg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa
     3421 gctcaaaagc tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg
     3481 tgacaataaa tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg
     3541 gttgtacgct ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg
     3601 atgcgatggt ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa
     3661 cgtttggcga tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc
     3721 aggtgacttc gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt
     3781 taacggcgac gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg
     3841 gagcagcgaa aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg
     3901 cgcttcccga ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag
     3961 ctgaggccag acgggtgctg gtggtggggc agggcggaga ggcagataac caggaaatcg
     4021 ttatgctggc agaaaagctg ggaaccgaag tgggctacag tcgggcgcga gtgatgaacg
     4081 gtggcgttga tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt
     4141 gcattgttgt tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt
     4201 ttatggtggc gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattgacg
     4261 tggttgatga ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc
     4321 aataacgtgc tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat
     4381 aagacgcgtt aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat
     4441 caaaacaaat tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag
     4501 actccggtat tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct
     4561 tcttcatcgc ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag
     4621 cccagcactt tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca
     4681 acttgcttat caatgttgtg aaacgaaatc gaatcaacaa taaacaggcg atgaatacag
     4741 aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc
     4801 gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc
     4861 aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg
     4921 atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt
     4981 tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag
     5041 aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg
     5101 cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc
     5161 caggcagccg cgatagtcaa tacgtaagcc accttcctgt gtgagagaaa aaagacctac
     5221 cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat
     5281 gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata
     5341 acatcgcccg ccagatgcca caggccatga cgacgtaaat gacgccagaa taactggcgc
     5401 agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac
     5461 gcaggccacg cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt
     5521 tgtagaacat cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt
     5581 tgcggctcgc ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc
     5641 agcgccatat ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg
     5701 cccaccagca gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct
     5761 ggcaccagat gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc
     5821 ggatgcgctt taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa
     5881 gagagcgggc aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg
     5941 ccgggtaagt catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc
     6001 agatgaaaac gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac
     6061 gccatcgctt cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg
     6121 gcaccttctg ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca
     6181 cagactctgc cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca
     6241 ccttcttttt cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag
     6301 gattcaccgc cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa
     6361 aggctttcgt gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt
     6421 tccgcgagtg catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca
     6481 gcgcgttcga gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg
     6541 cctgcaatac cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga
     6601 tatccagaac acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact
     6661 cttatcattc gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac
     6721 ttaacagcgg cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg
     6781 ttgggttttc cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg
     6841 gcttaaacgc cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt
     6901 ccccggtaat ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc
     6961 cttccgggac atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg
     7021 tggacatcat aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt
     7081 ttttcgcatt atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg
     7141 ctaaattcat ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct
     7201 aaaacaggtt agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt
     7261 caccccgtta accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc
     7321 tttgcttact ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc
     7381 gttgaacccg gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca
     7441 gcttgcctgg gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc
     7501 agtgacgcca gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac
     7561 cggcaatgcg cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa
     7621 cgttaagctg gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct
     7681 catcgtagtg acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca
     7741 taagttcctg ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag
     7801 cctcggcgat agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct
     7861 ggcggaactg ggtggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc
     7921 tgctgccagc gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc
     7981 ggcaccttcg caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag
     8041 caaagacgcg ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc
     8101 tgaaaaagac gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc
     8161 gggtgacgcg ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga
     8221 actgctgtgg ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa
     8281 cgaagcgcta cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta
     8341 cgccacgctt acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca
     8401 gcattacgcc gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga
     8461 tgccgaagcg ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc
     8521 ctcgcaggcg gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt
     8581 ggaaggccgc gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga
     8641 gggcgaagtc cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga
     8701 aaccccggtg attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca
     8761 gcaacgcgcc gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt
     8821 tacggaagac ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac
     8881 gcggatcgat ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact
     8941 gcgcgaacag ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg
     9001 cggcgacgct aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga
     9061 atttggtggc atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg
     9121 ccgttatcag cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa
     9181 ggaaaactga cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt
     9241 gcggaagatt taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc
     9301 cgcttccacg gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag
     9361 aagctggagc cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc
     9421 accaaacagt ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt
     9481 cgcctgacca accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg
     9541 gtgcaccaga tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac
     9601 cgtaacgtac tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag
     9661 tgggcgaaga agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg
     9721 ctcgatcagg aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacctacctg
     9781 ccgcgtaagt tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc
     9841 aacgacatga acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg
     9901 gtgggcggtg ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt
     9961 gagtttggct atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact
    10021 cagcgtgact ggggtaaccg aaccgatcgt aaaaatgcca aaacaaaata cacactggag
    10081 cgcgtggggg ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa
    10141 ccgatccgcc cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt
    10201 gatgataact ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg
    10261 cgtccgctga aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt
    10321 acggcgaacc agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag
    10381 aaaattgcca aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg
    10441 gcctgcgtgt cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg
    10501 tcttttatcg acaacatcga taatttaatg gcgaaacatg gtgtcagcga tgagcatatc
    10561 gtgatgcgtg taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc
    10621 ctggtgggta aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca
    10681 cgtatcccac ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat
    10741 gaactgatag ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg
    10801 gtgcgtgcgg gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca
    10861 tcagcccggt cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag
    10921 tgaggaatct atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg
    10981 cattctggcg ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt
    11041 tgcctgggcg ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca
    11101 ggcggcggtg agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac
    11161 cgacaccggc tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact
    11221 caagctcaac ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta
    11281 cggcaaactg tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt
    11341 cgaaccgatg aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg
    11401 gcgtgaacaa tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt
    11461 taaagtgctg ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca
    11521 tggcctgaaa tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac
    11581 aacccgtaaa tgggaacccg gcatggtgga agaagaaacg cgtttctttg gcttaaaaag
    11641 ggaatgtgga ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa
    11701 tgatgggggc gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg
    11761 gaaaattaac aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg
    11821 cgaggttcca gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc
    11881 gaacgtagca attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa
    11941 tatgcccttg tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta
    12001 ggtgactcgt tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc
    12061 gcttacgaat cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga
    12121 tggtttggtc tcctgcaccc ttttttaaaa tttatatttt ttatgtgaat atatagtctc
    12181 tatttattat tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta
    12241 ttggctgata tatatcacag atagttttta aaatatgcgt aaatatcctt taagtttact
    12301 gaaggataaa aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa
    12361 agagggtagc gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg
    12421 ctatttaatg gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat
    12481 ttctgacaag gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg
    12541 aaaatttgcc cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg
    12601 tgccagaaaa aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca
    12661 ttatttaagt gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag
    12721 gcgagtgata gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg
    12781 tatggatgaa ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct
    12841 tgaaatcaag ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg
    12901 cgttgaactt ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg
    12961 gacagggtcg tcaacgcgat tttttccacg cgtcgcacag gcaatagata ttgaagatta
    13021 ctggcaaaag gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga
    13081 aaccgcgcct ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt
    13141 acagcaaaag gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga
    13201 agatgtgacc ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc
    13261 tgcgggtaac gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat
    13321 gtacgatcga ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc
    13381 agcggagata aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg
    13441 atgcgatgat gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat
    13501 tttcaaaccc atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata
    13561 acgtcacggc agcgacgttc tattcttcct ggaagtgcct tatcaatatg ttgttgatta
    13621 tccagtctta cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc
    13681 tgataaccat ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg
    13741 cccgctttgt cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta
    13801 ttcaaacccg gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg
    13861 tcgcgcgtta atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta
    13921 tcgccaacaa ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca
    13981 ctcatccgct tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc
    14041 acaaatcgaa taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg
    14101 acacctaaac ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca
    14161 tctgcgtcag catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc
    14221 tgcggcgctt tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc
    14281 ttgcgattat cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga
    14341 taacccattt gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt
    14401 ccggtcatgc gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg
    14461 ccaggtttag gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca
    14521 gcggtacggt ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt
    14581 taccgccata gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc
    14641 gaaatccggt gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag
    14701 gaactactca ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca
    14761 tctgatggca agaaaatagc ggtattgcaa aggaacggtt atggatcaaa aacgacttac
    14821 tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt
    14881 ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc
    14941 gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg
    15001 gaaattccgc gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct
    15061 gctggtgcat aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag
    15121 cgcgaaacat accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg
    15181 ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg
    15241 aatctactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct
    15301 gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc
    15361 taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc
    15421 gctgtatctc gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga
    15481 tgacaaccgt atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg
    15541 tacgctgggc tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga
    15601 aatcatcgaa gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg
    15661 cgaccaggcg ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg
    15721 tacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac
    15781 ataaaagcct gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga
    15841 ttggtcgtct gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata
    15901 acgacagtaa gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg
    15961 gcctgcaagc tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta
    16021 ccgagaagcg taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata
    16081 tggcgactgg cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg
    16141 tgctcgatca aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg
    16201 tcgtggcgat caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc
    16261 gtgaagatta tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc
    16321 cgctctccgc actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca
    16381 gcggtctgac actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc
    16441 agccaatgcg cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg
    16501 ccggaatgct ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg
    16561 gtgtggaatc aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg
    16621 ccggagaagc gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc
    16681 tgctggcggc agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga
    16741 tggcggaaca gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga
    16801 cgcgtgctcg tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg
    16861 aagttgaaaa cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac
    16921 tggtgttaga tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc
    16981 tgagcaatgt gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg
    17041 cgtcatctga attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc
    17101 cgcactgggg cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt
    17161 cgtctggcat agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg
    17221 tgtagtgctg tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct
    17281 ggaggaggcg ttacataaac tcggcgtcag tacgtatctg ctggatggcg acaatgttcg
    17341 ccacggatta tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg
    17401 cgtcggtgaa gtggcgaatt tgatggttgc agccggactg gtggtgctga ccgcatttat
    17461 ctcgccacac cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat
    17521 tgaagtgttt gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata
    17581 taagaaagcg cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaaga
    17641 gcctgaatcg gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca
    17701 attattagat ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttca
    17761 atgcccggtt cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa
    17821 caaataacga cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg
    17881 tcggttttat ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca
    17941 cgctgttctt ctttatctac acctggcctt tctttctggc actgatgcct gtcgcggtag
    18001 tggtggggat tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca
    18061 ccctggtgac agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg
    18121 tactttcttg tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg
    18181 cacgccctgg gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt
    18241 atccccaaca gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca
    18301 gggggcagga tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat
    18361 tcgctgtggt tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg
    18421 gcactgcaag ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt
    18481 gacgatctca atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg
    18541 accaggccgg gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct
    18601 gcggggcaaa acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat
    18661 gtttgcgccg tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag
    18721 caatatctct caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg
    18781 catccccggg tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa
    18841 cttcctctgg cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat
    18901 tccgtgctgg caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc
    18961 gctcgtcctt gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc
    19021 cgcacgggag ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc
    19081 aaaaatgcca ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa
    19141 tttttccctc gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc
    19201 attaccgacg aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc
    19261 cgcgcggata acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc
    19321 acccgaacca tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg
    19381 cctttggcgg tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat
    19441 tgctggcgca ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg
    19501 cggcggcgct gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg
    19561 ccgacagccg cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc
    19621 ttggcaacgt cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac
    19681 aaatgcgcgt gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag
    19741 ccactactac ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg
    19801 tggcgctact cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg
    19861 taaaccgcaa ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga
    19921 tttgggcttt gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg
    19981 ctgcaatacc cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga
    20041 agtcagcttc gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg
    20101 cgtgccgggc aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct
    20161 ggagtatgcg cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac
    20221 cctggttctg cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg
    20281 cgcaaaaggt gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt
    20341 gcagggcgcg ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag
    20401 tttttggttg tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa
    20461 aaaagcagac gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag
    20521 ctggtttgta gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga
    20581 gctgatgata accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct
    20641 ggcattcgaa caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga
    20701 aaaagttgaa gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg
    20761 gtgggatgac gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag
    20821 cgttgtcagg gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg
    20881 acggggtaca tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg
    20941 ttcaggtggt cgcccccgat cgtaaccgca gcagcgcttc aaattctctg acactggaat
    21001 cctccctgcg cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga
    21061 ccgattgtgt ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt
    21121 ccggaattaa cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg
    21181 ccgcgatgga aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata
    21241 aacattacga cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc
    21301 cgctgcgcac cggacgtatt cttaatatta acgttccgga tttacccttg gatcaaatca
    21361 aaggtattcg cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc
    21421 aagatccgcg cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg
    21481 gtccggggac cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg
    21541 tggatttaac tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag
    21601 ttggcacgca atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg
    21661 tattcaggat gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga
    21721 tgaagcgttt gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac
    21781 aatttcgcag ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc
    21841 gcgggtgctg gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt
    21901 ccagcatgtt tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct
    21961 gaaaaatctt gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca
    22021 ggcacgtgcg ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc
    22081 gctaatgacg cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca
    22141 gtatttgaaa cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt
    22201 gcgctttgtc cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt
    22261 ttcctggtta ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg
    22321 gtgccttgca cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga
    22381 gcgcgggaag cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat
    22441 ggctggcagg ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca
    22501 atgcgcctgc aaatactaat tctggtatgt tgattacgcc gccgccgaaa atagggacga
    22561 cgtctacagc gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc
    22621 aacaaccgca aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg
    22681 gacgcatcgt ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta
    22741 cctataccgt gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt
    22801 tccgtgacct tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga
    22861 ccttgcaggt gggtaatgct tccggtacgc caatcactgg cggaaatgct attacccagg
    22921 ccgacgcagc agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg
    22981 cgtcgcaacc gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt
    23041 tgccgaacaa caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa
    23101 gcacaaccga gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct
    23161 ggccgactga gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga
    23221 ttgatatcgc aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt
    23281 atgctggtaa cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt
    23341 acctgagtgc ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg
    23401 cggggcaaaa aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg
    23461 aaattcgtta caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg
    23521 gcggaaccag gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat
    23581 gagtcagaat acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg
    23641 agttgaggtt tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc
    23701 cgaagaggaa ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta
    23761 ccttggtgag attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg
    23821 tcgcgcactg cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct
    23881 ggtggtaaaa attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga
    23941 agagggcaac ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg
    24001 cttctcaaca tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca
    24061 aacccgtact attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac
    24121 cgcacgtgag ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca
    24181 actggataag ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc
    24241 ggtagacacc ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga
    24301 aaaagagaac ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa
    24361 atggctgttc gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct
    24421 ggggtacgaa gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg
    24481 tgttcgccag attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg
    24541 gctgaatatc gaagcgctgt tccgcgagta agtaagcatc tgtcagaaag gccagtctca
    24601 agcgaggctg gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac
    24661 acgtcagcgt cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg
    24721 cataaacagc tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc
    24781 accaataggt atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg
    24841 tcgccgcttg tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc
    24901 caacatttct gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc
    24961 aataccaggt ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg
    25021 taactcgtgc tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatgaaacgc
    25081 tttatttcgt aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt
    25141 ttagtgacga agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta
    25201 tgttaataat aatgttcgta tacgaacaat taagagaata agcaatgaaa ctgatcgtcg
    25261 ggatgacagg ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga
    25321 tgccgaatgt cgagactcat ctggtgatgt cgaagtgggc gaaaaccatc attgaactag
    25381 aaacgcctta cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg
    25441 atcaggcggc gatcatctca tccggttctt ttcgtactga cggcatgatc gttattccgt
    25501 gcagtatgaa aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg
    25561 cggcggacgt cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc
    25621 ttagcaccat ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc
    25681 cgccgatgcc tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg
    25741 tagcccgcgt gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat
    25801 tgccgcaggc ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag
    25861 ctttttacag gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc
    25921 tgagccggat ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc
    25981 gctgtggttt gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg
    26041 ttcctggcag aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca
    26101 gattgatgag tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa
    26161 tccagcctgg gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc
    26221 gctgtttcgc ctgaatgatg gcgatggcgg tttctatctc gtatgactag gtaatttatt
    26281 caagataacc gggaagagat taaaaaagac gggaatagat tggaatcaac aagttaggaa
    26341 ctcaaggttt tcaagataaa gcgagtccaa aaatgttaaa cggttcgata tcaataaaac
    26401 atttaagata actcacaggt tcttacaata gcctttaaag accgttcaaa aacgattcag
    26461 gtgtttatta tagcgctgat gggtgcaagc gccaaaacca atgtaatttt agcacagcta
    26521 ggcacttgca ctgggttatg cgctggctgc tttctgaggt agatttaagt gatcgccctt
    26581 agcttcggac aacgcgtaaa tctctctaac cttttcgtct ggcaaattac caaggcttaa
    26641 cgctatggct ctacgaactc caactagagc gtgcaactct tggatgtgct catccagaag
    26701 aatgagtaat tgctccgctc cttttctccc aagaacatca gctacttgtt gtttttcttg
    26761 tggtttaaga aatgacaaga acattagaat ttgagcatca acattatccg cattctcttt
    26821 tttgggttct ttggaatctt ttagttcaac atcaccctct cccgtcatga gccattccaa
    26881 ggtaacattt tcgctttctg ctatcttttt taactgatgc gggcgtggta tcggtgcgta
    26941 atcccgcctg ttgtaataat tttttaaagt gccttcattg atcccccatg cccgagcagc
    27001 cgcatttctt gaagggtatc gttctactaa cctaaagatt ctttcgataa tcggttcttt
    27061 tccatcggca ggaaaagaac cttggggtgc ttttctcatt cttggttcct ttctatctaa
    27121 ttcattgata aataatgaaa ttaaaaaata aaccataaaa gcaccaaaga accctttata
    27181 gggaatcttt ggtatcttaa tctttatcca tacggatatc cgcgcgggtt attttgtagg
    27241 ttaaaggaaa gatgagacaa agagaagata gtgaccgcga ttggccttcg ctgaagattc
    27301 agctggcgct caaagataac gggtggtctt ccttccggga gctttcactc catgctggtt
    27361 atgctcagga ttcactgaaa gcggtagttc gcacaccaaa caaaaaatac gaacgcgtaa
    27421 tttctgacgc tataggtgta ccgccagaaa tcatctggcc cagccggtat cgtaacgatg
    27481 gatttatcag gaaggcgtcg tgatatgcag tcgtacgtta ccgtaaatga cctgctcggc
    27541 gtaccaggaa tgccagccac cacaaaaggc atccgacagg cgttacagcg tttttctcgt
    27601 gatcttggtg acgttagtcg ccgtcgggaa ggcacaaaag ccattgagta ccacattgat
    27661 tgtctgcctg aaataacacg aaaggcactg cgtgaacgct atgtggaaca gctggtggct
    27721 acagagaaca atgtttctga agtgaaagct gttaccagaa aaacacgcaa tcctgacgct
    27781 gtccaggcaa tcgaagcata tcgcggttct ccacaactga tggaagaacg cctgaatgcg
    27841 ctgaccgaaa accagcgctg ggtatctgaa gcaagagctg cgctggtggt ggaagtgctg
    27901 aagctggaaa gcgccggtaa cctcgggcga ctgaaagcca ttaactttct tgttgaaaaa
    27961 gcccgtaaag gtgagctgcc ggagcgcctg caacaggccg cagttaacgc caatgcaaaa
    28021 cgtggcgcta atcgtactat cagccgcgat ccactttacc agtgggttct gaaatacaac
    28081 cagtcgcaga acgccgccga acggctgctg ttactggcac cgggaaaacg cgacgaaata
    28141 aaaccggaag aaatcagctg gctgcctgaa tttctggcgc agtatcgcca ggtgaacggc
    28201 agacccatgt cagaagccta cgaggatttt gtcgcagaat ggcagcgtcg ccatgcggac
    28261 gaaccgtaca tgctggaggt gatgccttcc tatgatgttg tccggtatgc catgaaaaaa
    28321 ttgccggaag tggtgaaaca aaaaggacgc gttaccggca gtgaataccg ccagcttgag
    28381 ggattcaccc gccgcgactg gacagctatg ccggtgaatt atgtctggat tggtgacggt
    28441 cacggtatga agctgaaatg tgcgcatccg atccacgggc gtccattctc accggaagtc
    28501 acgtttgtga tcgatggtgg aacacggttt gttgttggct ggagtcttga ccttgccgaa
    28561 aacgtgttcg ccgttgccgg tgcgatacag cacggcattc gcaaccacgg aaaaccgttc
    28621 ctgtattact cggataacgg ttccggtgaa acggctgaca tgctggacaa agaggtcgtg
    28681 ggtattctgc cacgcctggg gattaaacac cctaccggta ttgccggtaa tccacaggga
    28741 cgcggcatta ttgaacgcct gaaccggaca ctgccgatgc gtatagccag gaggtatcgc
    28801 acctattttg gcaagggtgc tgaccgggaa tcgttgcgtg tacttaaccg tgatctgcgt
    28861 tcagcgttta acgccctgca acaggacaag cctctgaacg accgccagaa agcggcaatg
    28921 cgcgagctgc catcgtgggc ggagcttatt gaggctattc gtgaaggcgt ggaatggtac
    28981 aacaaccgcc cgcattctga gctgccgatg aaaccgaacg gacggcatta cagcccgaca
    29041 gagttcagaa aaaaacgcct ggcagaagaa gacaccgaaa ttgagtggct gagtgatctt
    29101 gagctgcgcg acatgttccg cccaatggtg gaacgaccgg taagacgctg cgaaattcag
    29161 tggctgaaca atatttacta cgcgccggag ttgcgtgacg aacacggtcg caaagtgctt
    29221 atcagttatg acattcatga cgccgagcga attacggtac gtcgcaagga cggcagcttt
    29281 atctgtgagg cgatatggaa cggcaataaa cgcgcagcct ttgctgtcag tgctgaatat
    29341 cacaaacaac agcagcgtat taaaggaatg cgcaaacgcg ctgaggaaaa aattcgtgat
    29401 gctgaagatg aaggtattca gattctggaa cacaaacagg ctgaaccctg gctgagtaat
    29461 gtttatcgcc cggttggtaa tgtggtggca gttcagcaac cggaatatga agaagagcat
    29521 gacgaagaat ttgaacgcga tttccggctg gggatgcaaa aactttttgc catgcaggaa
    29581 gaagatgatc cgctggcctg aataaaaaaa cagtccgcgg gaacggactg ttaaataaag
    29641 gaaacaaagc tgatttgaaa taaccgagga aatactatga ctgatattga tgat
//
LOCUS       DADLTI010000034        29141 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLTI010000034 DADLTI010000000
VERSION     DADLTI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.034"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..885
                     /locus_tag="JN755_001375"
     CDS             <1..885
                     /locus_tag="JN755_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7380227.1"
                     /translation="FAVLIFILLITRYAKGFMSNVAVLLGIVFGFLLSWMMNEVNLSG
                     LHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFLALGEIVGRKLSSHD
                     IIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVP
                     KMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTP
                     TLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLKVR
                     TVRMWLLMRKLKKNEHGE"
     gene            887..1012
                     /locus_tag="JN755_001376"
     CDS             887..1012
                     /locus_tag="JN755_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380228.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1009..1080)
                     /locus_tag="JN755_001377"
     CDS             complement(1009..1080)
                     /locus_tag="JN755_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380229.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1135..1683
                     /gene="idi"
                     /locus_tag="JN755_001378"
     CDS             1135..1683
                     /gene="idi"
                     /locus_tag="JN755_001378"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7380230.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(1726..3243)
                     /gene="lysS"
                     /locus_tag="JN755_001379"
     CDS             complement(1726..3243)
                     /gene="lysS"
                     /locus_tag="JN755_001379"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7380231.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(3253..4351)
                     /gene="prfB"
                     /locus_tag="JN755_001380"
     CDS             complement(join(3253..4275,4277..4351))
                     /gene="prfB"
                     /locus_tag="JN755_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7380232.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(4442..6175)
                     /gene="recJ"
                     /locus_tag="JN755_001381"
     CDS             complement(4442..6175)
                     /gene="recJ"
                     /locus_tag="JN755_001381"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7380233.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6181..6891)
                     /gene="dsbC"
                     /locus_tag="JN755_001382"
     CDS             complement(6181..6891)
                     /gene="dsbC"
                     /locus_tag="JN755_001382"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7380234.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(6916..7812)
                     /gene="xerD"
                     /locus_tag="JN755_001383"
     CDS             complement(6916..7812)
                     /gene="xerD"
                     /locus_tag="JN755_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7380235.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7924..8445
                     /gene="fldB"
                     /locus_tag="JN755_001384"
     CDS             7924..8445
                     /gene="fldB"
                     /locus_tag="JN755_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7380236.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(8485..8892)
                     /locus_tag="JN755_001385"
     CDS             complement(8485..8892)
                     /locus_tag="JN755_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380237.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(8873..9139)
                     /gene="sdhE"
                     /locus_tag="JN755_001386"
     CDS             complement(8873..9139)
                     /gene="sdhE"
                     /locus_tag="JN755_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7380238.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            9382..10362
                     /gene="ygfZ"
                     /locus_tag="JN755_001387"
     CDS             9382..10362
                     /gene="ygfZ"
                     /locus_tag="JN755_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7380239.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(10558..11217)
                     /locus_tag="JN755_001388"
     CDS             complement(10558..11217)
                     /locus_tag="JN755_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7380240.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(11381..11692)
                     /locus_tag="JN755_001389"
     CDS             complement(11381..11692)
                     /locus_tag="JN755_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7380241.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            11737..13169
                     /locus_tag="JN755_001390"
                     /pseudo
     CDS             11737..13169
                     /locus_tag="JN755_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            complement(13226..13969)
                     /locus_tag="JN755_001391"
     CDS             complement(13226..13969)
                     /locus_tag="JN755_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7380242.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(14236..17109)
                     /gene="gcvP"
                     /locus_tag="JN755_001392"
     CDS             complement(14236..17109)
                     /gene="gcvP"
                     /locus_tag="JN755_001392"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7380243.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(17228..17617)
                     /gene="gcvH"
                     /locus_tag="JN755_001393"
     CDS             complement(17228..17617)
                     /gene="gcvH"
                     /locus_tag="JN755_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7380244.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(17641..18735)
                     /gene="gcvT"
                     /locus_tag="JN755_001394"
     CDS             complement(17641..18735)
                     /gene="gcvT"
                     /locus_tag="JN755_001394"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7380245.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19026..19109
                     /locus_tag="JN755_001395"
     CDS             19026..19109
                     /locus_tag="JN755_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380246.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19182..20384)
                     /gene="ubiI"
                     /locus_tag="JN755_001396"
                     /gene_synonym="visC"
     CDS             complement(19182..20384)
                     /gene="ubiI"
                     /locus_tag="JN755_001396"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7380247.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALILAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(20407..21585)
                     /gene="ubiH"
                     /locus_tag="JN755_001397"
                     /gene_synonym="visB"
     CDS             complement(20407..21585)
                     /gene="ubiH"
                     /locus_tag="JN755_001397"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7380248.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(21582..22907)
                     /gene="pepP"
                     /locus_tag="JN755_001398"
     CDS             complement(21582..22907)
                     /gene="pepP"
                     /locus_tag="JN755_001398"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7380249.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(22933..23511)
                     /gene="ygfB"
                     /locus_tag="JN755_001399"
     CDS             complement(22933..23511)
                     /gene="ygfB"
                     /locus_tag="JN755_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7380250.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23679..24008
                     /gene="zapA"
                     /locus_tag="JN755_001400"
     CDS             23679..24008
                     /gene="zapA"
                     /locus_tag="JN755_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7380251.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24050..24233
                     /gene="ssrS"
                     /locus_tag="JN755_001401"
     ncRNA           24050..24233
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN755_001401"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            24254..24856
                     /locus_tag="JN755_001402"
     CDS             24254..24856
                     /locus_tag="JN755_001402"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7380252.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24953..25012)
                     /locus_tag="JN755_001403"
     CDS             complement(24953..25012)
                     /locus_tag="JN755_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7380253.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(25345..25404)
                     /gene="ibsC"
                     /locus_tag="JN755_001404"
     CDS             complement(25345..25404)
                     /gene="ibsC"
                     /locus_tag="JN755_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7380254.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(25633..26865)
                     /gene="serA"
                     /locus_tag="JN755_001405"
     CDS             complement(25633..26865)
                     /gene="serA"
                     /locus_tag="JN755_001405"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HAY7380255.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEIAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27121..27780)
                     /gene="rpiA"
                     /locus_tag="JN755_001406"
     CDS             complement(27121..27780)
                     /gene="rpiA"
                     /locus_tag="JN755_001406"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7380256.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28163..29056
                     /locus_tag="JN755_001407"
     CDS             28163..29056
                     /locus_tag="JN755_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7380257.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         6935 a         7873 c         7319 g         7014 t
ORIGIN      
        1 tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt tatgtccaac
       61 gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat gaatgaagtc
      121 aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat gtcgtttggt
      181 atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat catcgtgttt
      241 atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa actctcttca
      301 cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg cggcacgttt
      361 aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt gatgcgcgtt
      421 catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg catggtgcca
      481 aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc tggtctagtg
      541 atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa ctacaccacc
      601 aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac tccgacgctc
      661 tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgctgctaca tagcggcatt
      721 atgctcgcaa cccttagcgc cgttgtgctg aacgtcttct ttaatggcta tcagcatcat
      781 gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac agtacgtatg
      841 tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga attttttaat
      901 gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca tttctgactg
      961 tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt aaatggtttt
     1021 tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctctctt ggttaatcat
     1081 ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga aattatgcaa
     1141 acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat
     1201 gccgcacaca cggcagacac cctcttacat ctcgcgttct ccagttggct gtttaatgcc
     1261 aaggggcaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc tggcgtgtgg
     1321 actaactcgg tttgtgggca cccacaactg ggagaaagca gcgaagacgc ggtgatccgc
     1381 cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt
     1441 cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc
     1501 gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta tcaatggtgt
     1561 gatttagctg atgtattacg cggtattgat gccacgccgt gggcgttcag tccgtggatg
     1621 gtgatgcagg cgacaaatcg cgaagccaga atacgattat ctgcatttac ccagcttaaa
     1681 taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt tcaccggacg
     1741 catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca gcattaccat
     1801 acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca gtgcggtaac
     1861 gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg caacctgatc
     1921 caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt taccgatttc
     1981 acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt cgttacgacg
     2041 cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa tcagatgtgc
     2101 ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc tcttctcaac
     2161 gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca gatccgccat
     2221 gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt cgaacggttt
     2281 accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa tatcctgtgc
     2341 cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg cgtaagccat
     2401 gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt cgttacggaa
     2461 gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct tgaggtacag
     2521 ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg tgataaacgg
     2581 acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt ccataaagcc
     2641 acggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt taaaggtgtt
     2701 acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg cttcctgatc
     2761 ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca gacgcagctc
     2821 ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt tcgcgccgag
     2881 gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg gcagatcgtc
     2941 acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga aagacgcttt
     3001 acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt tcagcgcttc
     3061 cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg tatgatcgcg
     3121 acggaaatcg ttcgggaagg caattccctg ctcacgcagg ttcgccagct tctcacgacg
     3181 cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt gttgttcaga
     3241 catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat ccaggctgcc
     3301 gtccagcacg gcctgcgtgt tgcgagtttc taccccggtg cgcagatctt taatgcggga
     3361 gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt tgttatcttc
     3421 catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct tcgctttcat
     3481 ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc actgggtcac
     3541 gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa cgtgctgacc
     3601 gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga tttcgatatc
     3661 aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt ggcgacgacc
     3721 gccggagtca aacgggcttt tacgcaccag gcggtgaacg cccgtttctg tacgcagcca
     3781 gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg ccacttcacc
     3841 ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc gcagatacat
     3901 acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg cctgaatatc
     3961 gaggtaacag tcggcgctgt catattcgcc agagaacata cggcggaact caagctgcgc
     4021 cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt cttcgtcgtc
     4081 agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt tcatttggtc
     4141 gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct gtgcgcgttc
     4201 gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca gacgctcttt
     4261 cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg aggtcctgaa
     4321 tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg agctagtcaa
     4381 aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag agaagatgac
     4441 gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg cgaaactcgt
     4501 tgatatcgag cttataagcc agttgcactt cgcggacgcc gttatccggc cagagagcgg
     4561 tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt tcgaccatca
     4621 ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga ccgtcaaaca
     4681 gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc gccacttcca
     4741 tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc aatagcgaag
     4801 gggccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg aatttatcct
     4861 cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttgagcatc atgccggggt
     4921 agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg gagcgaccgg
     4981 aacctttcag cgtaccgtca cctgctggcg caaaggcgat aaccggacgg tgaaaacgct
     5041 ctttgatacg cgaagccaga ataccgacaa cgccctgatg ccattcgggg tgatacattg
     5101 ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg gtcagggctt
     5161 caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg agttcatttg
     5221 ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacacca acggacatat
     5281 catccagtcg cccggcagca ttgagacgcg gtcccagcgc aaaacctaaa tcgctggcgg
     5341 ctagtttttg tgcatcgcga tttgccactt ccagcagcgc tttaatccct ggacggcact
     5401 ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta gcgtccagcg
     5461 gcacgacgtc cgctactgtc cccagcgcta ccagatccag cagttctgcc aggttaggaa
     5521 ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc agcgccagca
     5581 tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta cagtcgcgca
     5641 agttagggtt aatgatcgct tccgctgcgg gtaatgtgtc gcccggcaaa tggtgatcgg
     5701 taacaataac cgggatgccc aacgagcgag cgtgctcaac tcccgcatgg gaggaaatac
     5761 cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg accacttccg
     5821 ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata ttgctgcaac
     5881 caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg gcgtcgaaat
     5941 caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc tcaacggcct
     6001 tttcgacacc gcttagttgc tgccagggca acataccttt aacactgcgt tccagttctt
     6061 gcgcactgcg tactccccgg ctggcgtata aacggcgcag caagggaggc aattcagcgg
     6121 gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt ttcacgcgaa
     6181 ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatgt ctttcggcgg
     6241 ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac cgctaacgcc
     6301 aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg ctggtgcgac
     6361 gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac accagatagc
     6421 tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa gataacgcac
     6481 ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc agtaaccaca
     6541 ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat aaacgatcat
     6601 ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat tgaccggagc
     6661 cgtgccacta acgtcataca ttggcccctg aatgatatgt ttaccatcat cggtgatgta
     6721 caacacaccg ctgttagtca gaactgtctt catgccagct acgggcgcgg gctgaatatc
     6781 gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat cagcctgagc
     6841 aaagcctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca taaatcttcc
     6901 cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc gcagacgctc
     6961 ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca gtagcatctg
     7021 caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt gacgcaacac
     7081 atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt taatgcggtg
     7141 ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca cgtcaattga
     7201 cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc agtaaaccgc
     7261 ctcttcccct aacggcacca ggcgctcttt gttgccttta ccaatgaccc gcactacgcc
     7321 ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac gcagcccggt
     7381 agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat caattaatgg
     7441 cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac gctggggcaa
     7501 tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc gataaagata
     7561 ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt tatacccgcc
     7621 ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg ccagcgtcaa
     7681 cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag cgttcaacgt
     7741 attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga tgcgtgccag
     7801 atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc ttattgtgcc
     7861 gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg agaagcttac
     7921 gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat ggcggcagaa
     7981 aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa ggacgactcc
     8041 ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg ggattttggt
     8101 gaaatccaag aagactggga agccgtctgg gatcagctcg acgacctgaa ccttgaaggt
     8161 aaaattattg cgctgtatgg gcttggcgat caactgggat acggcgagtg gttcctcgat
     8221 gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt cggctactgg
     8281 ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg gcaactgttc
     8341 gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg tattcagagc
     8401 tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca ctgacggctt
     8461 agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca aaatccgccg
     8521 taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat gttgccgttt
     8581 accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg gtgctttgac
     8641 gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc gaatttcccc
     8701 ctggcgagca ttaatacacc gctggctgcg aacgcaatca aataccacca gcgaaagtaa
     8761 caccatccat aacggggtgt aactgagcgg ccagggcatg agtaaaataa cagcggcaac
     8821 cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca aatcagattg
     8881 ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccattatt tccagttctg
     8941 catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg tcacattcca
     9001 gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca tgttcgaaaa
     9061 acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa tgaatgcggg
     9121 ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc ggctattgta
     9181 acgtgttttt cgacttcttt tacgggaata tcagtaaaca ccatcgcgat cgcgaaatta
     9241 attcacaaca attcaagggc ttcatttttt tggcagtcgc ctcgcagaag gcacagatcg
     9301 cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt aatacgccgt
     9361 taagcaactc aggactctat tatggctttt acaccttttc ctccacgtca gcctacggct
     9421 tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc caccattact
     9481 ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag ccagatggca
     9541 gaagatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg gagcaattta
     9601 cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt gcgtgaaccg
     9661 cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc gccagacgac
     9721 gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc aaatctcttt
     9781 agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac tttgctatgg
     9841 tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc taatatgctg
     9901 accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct tgcattaaac
     9961 attgaagctg gtttcccggt gattgatgcc gccaacagcg ggcagtttat cccacaggcg
    10021 accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac cggacaagag
    10081 atggtggcgc gagcaaaatt ccgtggtgcc aacaaacgcg cgctctggtt gctggcaggt
    10141 agtgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg cgagaactgg
    10201 cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt cgtggtacag
    10261 gtcgtcatga ataacgatat ggaaccggac agcatcttcc gcgtacgcga cgatgcgaat
    10321 acactgcata tcgagccgct gccgtattcg ctcgaagagt aaatctctgt atcgcatcag
    10381 gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt gatgccttta
    10441 tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgcatc cggcggttgc
    10501 gctccgatgc ctgatgcgac gctgacgcgt cttatcaggc ctacaaaggc atacccatta
    10561 cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc cgagcacgaa
    10621 gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa tcaccccgag
    10681 cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga gcttaactgc
    10741 catttcataa attaccacca gcgacagcca gcccatcgcc agataagtca ccagagataa
    10801 aattttgaat cggtgcgcga tggtcagttt gaacagaatc cccagcaatg ctaggctcca
    10861 gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca aaaacggcgt
    10921 gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca gccacatttt
    10981 tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca ggatcatact
    11041 gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat ccaccgcctg
    11101 aaccagtagc aacaccagcc caacgatacc aaacaccagc ccaatgccgt gactgacgct
    11161 gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct gaaccataac
    11221 ttactccaga gaaacgtaca cgcacatgta tacctctcca gcgtaactga gaatagttcc
    11281 agtgaacacc tgttagctaa aataaatttt gatttgagac taatctccta aaaatcatga
    11341 aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat ttaaagacat ttaaattcaa
    11401 tcatataaaa ttgtgtctga ccgggataga tgtcagcaat gacctttttc agttcaggca
    11461 gggtcatatt ttcctgctct gcatgttttt ccgtcagtgt atccagcgtt acggttgagg
    11521 ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg acacgaagca
    11581 catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt ttacgcccag
    11641 ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc atggtactat
    11701 ttcctgtaag aattgactga tctggagcct atgatagtga aaaaactcac cttaccgaaa
    11761 gatttcttat ggggtggcgc cgttgccgct catcaggtcg aaggcggctg gaacaaaggc
    11821 ggcaaaggac cgagcatttg tgacgttctg accggtggcg cacacggcgt gccgcgcgaa
    11881 atcaccaaag aagtcttgcc aggaaaatac tatccaaacc atgaagccgt tgatttttat
    11941 ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg ttttcggact
    12001 tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa cgaagaaggg
    12061 ctgaagtttt acgactctct gttcgatgaa ctgctgaaat acaacatcga accggtgata
    12121 accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag ctggaccaac
    12181 cgtaaattcg ttgatttctt tgtccgtttc gcggaagtgg tattcgaacg ctacaagcat
    12241 aaagtcaaat actggatgac cttcaacgaa ataaacaacc agcgtaactg gcgtgcgccg
    12301 ctgtttggtt actgctgctc cggcgtggtg tataccgagc atgacaaccc ggaagagacg
    12361 atgtaccagg tgctgcatca ccagtttgtc gccagcgccc tggcagtgaa agccgcgcgt
    12421 cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct ctatccctac
    12481 tcatgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg ctacgtcttt
    12541 accgatgtgc agctatgcgg ctattacccg tcctatgtat tgaacgagtg ggaacgtcgc
    12601 ggatttaata tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg cacctgcgat
    12661 tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg caccggcgat
    12721 gcgatctctg gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc tgactggggc
    12781 tgcagattga tccagtaggt ctgcgctatg ccctttgcga actgtatgag cgttatcaga
    12841 ggccgctgtt tattgtcgaa aacggttttg gcgcttacga caaagtggaa gaagatggca
    12901 gcatcaacga cgactaccgc attgactacc tgcgcgccca tattgaagag atgaaaaaag
    12961 cagtgactta cgatggtgtg gatctgatgg gctacacacc gtggggctgc atcgactgcg
    13021 tgtcattcac taccgggcag tacagcaaac gctacggctt tatctatgtg aataaacatg
    13081 acgacggtac tggcgatatg tcgcgttcac gtaagaagag ctttaactgg tacaaagagg
    13141 tgattgccag caacggcgag aagctttaag tcgatgaagt accggatgca atacttgttg
    13201 catccggtca gacaacttcc ctgttttatt tcccgcccgc caaatcgata aaacttcccg
    13261 tgacgtaaga ggctttatca cttagtagcc agacaatggc ctgcgcgacc tcttctgcct
    13321 gtccaccacg ctgcatgggg atgttcgact taacgcgatc gacgcgtcca ggctcgccgc
    13381 cgctggcgtg catttcggta taaataaacc ctggccgcac gcagttaacg cggatcccct
    13441 gcgcggcgac ttccagcgat cgtccggtgg ttaacgtatc aatcgcccct ttcgatgccg
    13501 cgtaatcaac atattcccct ggcgaaccca accgtgaggc caccgaagag acattgacga
    13561 tagcgccgcc actgccacca tttttaagcg ccatgcgttt taccgcctcg cggcagcaga
    13621 gaaaatatcc cgtcacgttg gtggaaagta ctcggttgat tcgctctgcg gtaaggtttt
    13681 caacggtgca ctgggtaaac aagatcccgg cgttattgac cagcgctgct agcggttcat
    13741 cgtgctgatc gattgctgta aacatcgcaa cgacctggtt ttcgtcgctg atatccgcct
    13801 ggagcacgaa tgctttgcca ccggcttgca ttattaagtt catcacttcc tgcgccgcgt
    13861 ggaggttttg ctgataatta accgccaccg tatacccttc ttgcgccaac agtaatgcag
    13921 ttgcccgccc gatgccgcga ctgccaccgg tcacaagtgc tatagccatt tgtttttccg
    13981 agaaaaatta attcgttgac gtatctttga gattgctaac aaactggctc aatcttcaat
    14041 gttggcacgc ttttccccct cgccctttca gggagatggt cggggtgagg gtaaatattc
    14101 gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggactg
    14161 tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc tttagaaaat
    14221 actcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga acaggttacg
    14281 gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg ccacgcctgc
    14341 cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca gttcgctctg
    14401 aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacaccgg ctttcacctg
    14461 gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt ccactttgct
    14521 ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca tcgtcggcgc
    14581 gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc cgatttcttc
    14641 tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc gaccggtata
    14701 cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga ggattgccac
    14761 ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc agctgattgg
    14821 caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg ttaacatgcc
    14881 ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt tcacaccgat
    14941 cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat gcaggttgag
    15001 gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg cgttcatgtt
    15061 cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca cttcacggat
    15121 cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg agaggttatc
    15181 gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt ttttatcaca
    15241 cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag ttccgtgcgc
    15301 agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat gataatgacg
    15361 aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca tacaaacggc
    15421 gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct gctgataacc
    15481 ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccgtcc aagtgattgg
    15541 gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa cccagcggga tcatcgcctg
    15601 attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt cggtttcgct
    15661 gtgatagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca tcgcaggctg
    15721 gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca gcccgtggtt
    15781 atcgcccagc agcacactga aaagctgcat tacgttttca cgcgtggtgg tttcatcaag
    15841 ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg cttcggcacg
    15901 cgtcagtacg cccgctttgt cggccacttc cacacacaag gtgtcgaaat agtgcgcatg
    15961 gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca gacggtgaat
    16021 gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca ggctggcgat
    16081 gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac gacgaatatg
    16141 ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat ctttcgatac
    16201 accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa agaatgccgc
    16261 gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa aaacaatatc
    16321 cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg cggcaacgct
    16381 gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt gaatttcacc
    16441 ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga gcactttttg
    16501 cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga ccacatccag
    16561 tgtttgcgga tgcacattgg aagccacgaa gaagcggttg gcatttttca gtttgctgac
    16621 gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa gagaagcaga
    16681 ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca gtgcttcaag
    16741 gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc ccggattttc
    16801 cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca tgccgatgta
    16861 agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg cggcgtattc
    16921 ggtcgccggt gcgccaacct gcggtggtgt cgcaagctga atatctttcg gcacaatctg
    16981 gccggtcagc gcgtttaacg attgtgcacc aacggcattc agcatttctt gctgttgcgc
    17041 ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct ggcttaacgt
    17101 ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct ctccttacga
    17161 agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca gaagaggaat
    17221 aaagccgtta ctcatcttct aacaatgctt cgtatgcggt cgcatccagc agtgattcca
    17281 gttcgctttc atcgctagct ttgattttaa agatccagcc gcctgcatac ggttcgctgt
    17341 tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca ccgcttactg
    17401 gcgcataaat gtctgacgcc gcttttaccg attcggcaac cgcgcagtca tcgcccgcgc
    17461 taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac agctcctgag
    17521 catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc cattcgtgtt
    17581 ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca aaaaagtaaa
    17641 tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa ccggcatttc
    17701 acggttgcga atttgcacaa tcgccgtttc gccaatacct tccggcacgc gcgccagcgc
    17761 aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt catgctggtt
    17821 gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt tttcggtcat
    17881 caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg cttcacgacc
    17941 gataaagtca cgatctgtcg gttcccaggc gatagtccag cccatgttgg cggctaaagg
    18001 agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca gacgcagcgt
    18061 gtcacgcgcc cccagaccgc agggcttaac gcccgcttcc accagcgcac gccagaaatc
    18121 ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat aaccagtggt
    18181 ggcaataaac agatcgccca cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg
    18241 acgctgagca tcattaaaca gtgtggcagc ttttgcctga gcattcggcc cttgcacggc
    18301 aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag cgtgttgggt
    18361 aatccaggag aggtcttttt cgcgggtggc ggagttaaca atgaggcgga agaaatcttc
    18421 agtaaagtag tagacgatga gatcatctat cacaccgcca gaggcattca acatccccga
    18481 gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca gataacgcag
    18541 aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgcgaca catcaaacat
    18601 tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat gcagcggcat
    18661 catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg
    18721 agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt ttcatggtga
    18781 aattatcacc acaaaaccca gcatcggagc cactcccggt ccccaacgca atcgttctct
    18841 tttgcctgaa cttaccaccg aaacagactg ttaaccataa ggtaaaattg atcatcacat
    18901 tagcttatgg ttaaaaaatg caaaaatcgc gacagaataa aaaaccaaaa aatacaccaa
    18961 tttctataca aagatgatgt gatgagaaag tcaatttgaa taagacaata ttaagagcta
    19021 aaaaaatgtc aaaaaacact aaatcaaaaa atcatggcat tagaaaatat aatgcgaaaa
    19081 cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa ccgggagatg
    19141 aaagtgtgat gggtatcaaa taaacaacag aggagaaatt tttaacgcag ccattcagac
    19201 aaatcgttta atcccattgc ctggcggata agttgcggct taacgccagg aagcgtgtcg
    19261 gccagtttca aaccaatatc acgcagcagt tttttcgccg gattagcgcc ggaaaagaga
    19321 tcgcgaaaac cctgcatacc agccagtatc agcgccgcgc tgtgcttgcg gctacgttca
    19381 tagcggcgca ggtaaatgta ctgcccgatg tctttaccct gacgatgcaa ccgtttcagt
    19441 tcagcaatca gctctgcggc gtccataaat ccgagattaa cgccctgccc cgccagtggg
    19501 tgaatagtat gcgcggcgtc gcccaccagc gccagacggt gtgcggcaaa ctggcgcgca
    19561 taacgccccg tcagcgggaa cacctgacgc gcgctctcaa ccttgcataa gccaaggcga
    19621 ttatcaaaag cgatatttaa cgcgcgatta aattcgtctt cacttgcctg ctgcatccgc
    19681 tgcgcttcct ctggcgacag tgaccagaca atcgagcaaa gatgcggatc gctaagcggt
    19741 aaaaaggcca gaatgccttc gccatggaaa acctgccgcg ccaccgcatc atgcggttct
    19801 tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat cccagaaagt cagcggaata
    19861 tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc caatcaccag acgcgccgtt
    19921 aacatgctgc catctttcag cgtcaggaag gtttcatttt ctccccaggc gacctgctgt
    19981 aattctgtgg gggccaacag agtaatatct gacgactgct gcgctttgtt ccacagcgcg
    20041 tagtgaatca ctgagttttc aacgatgtgc ccaagatggc tatagcccat gctttgatcg
    20101 tcaaacgaga tgtgaccaaa actgtctttg tcccacactt ccatgccgtg atagcaactg
    20161 gccctacgag cgagaatgtc ctgccagacg ccaagacggg tgagtaattt ttcgctggcg
    20221 gcattgatag ccgaaacgcg cagttgtggt agtgcatcta ccgccagagg ttccggtacg
    20281 cgctgctcca gtacggcaac gcgtaagccg ctcccctgta agccgcaggc aaccgccagc
    20341 cccaccatgc cgccgccaac aatggctaca tcaacacttt gcattgttta ttccttaaaa
    20401 ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc agcacatcgc gtgccggggt
    20461 gaataattcc atcgtcatca gtccgatgtt gcgcccgaca accagcggtg cccaacggtt
    20521 ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt gcttcgcgat cgctctgtcg
    20581 acgctgctga tagcggcaca atatgccgta atcaccgatg tcttctccgc gctcatgcgc
    20641 ctgagtcagg gtttccgcaa ggctcatgac atctcgcata ccgaggttaa acccttgccc
    20701 ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc acagtgcgat gggtaatcgg
    20761 tctggcggcg cgggttaatg ccagcggata agcactgcgt ttaccggcgt gggtaatttg
    20821 ccccagtcgc cagccaaagg cagactggag ttcacggcaa aacttctcgt cgctccacga
    20881 cagcacctct tcgcgccgtt ccagtggatg acaccagacc agcgaacagc gtccgtcaga
    20941 catcggcaac atcgccagcg ggccatgttg cgtgaagcgt tcaaaagcgc ggccttcgtg
    21001 cgcaacagaa gtagcaacgt tggcaatcac tgccagttgt tcgtaaggct cctgctgcca
    21061 atcaacgccg cacgcggtgg ctaacgctga atgggtgcca tcagcggcta tcagcacgcg
    21121 gcccgtcagc atctcgccgc tctccagcgt cacttcaacg tgactctgag tacgggcaac
    21181 gttagctacg cgatcggggc aatgcagcat tacgccaggt gcttttcgca gcaatgcaaa
    21241 cagccgttgc ccaacattat gcaattcgac aacctgcccc agcgccgcca gttggtaatc
    21301 ttctgcggcg agggtgacga atccagcatg accacgatcg ctgacatgca cggtagtgat
    21361 ggcggttgcg caatcagcca gagattgcca gacgccgatg cgcgccagtt gctgacaggt
    21421 acccgccgcc agcgctatcg ctcgtccatc aaagcccgga tgagcatgtg actctggcgc
    21481 agtcgcttca atcaaatgta ccggcagcgc cccgtgactt aaccgggaaa tagccagcgc
    21541 cagcgtcgcg cccgccatgc cgccaccgac gatgattacg ctcattgctt tctcgcagca
    21601 gccatcaacg cttcgatttc ttccggcttt ttcaccacgc tggcggtgag gttttcgttg
    21661 ccggtttcgg taatcacaat gtcgtcttca atacgaatgc cgataccgcg atattgttct
    21721 ggcacatccg catccggcgc gatatacaga ccaggctcta cggtcagtac catccccggt
    21781 tccagaatgc gcgagcgatc ctgaccataa acgccaacgt catggacatc cagtcctaac
    21841 cagtggctaa ggccatgcat aaagaaagga cgatgggcgt tctgagcgat cagttcatca
    21901 acattacctt tcaggatgcc gagttttacc aggccgctaa ccatgatgcg caccacttca
    21961 ccagtgactt ccagaatgga agttcccgga cgatacaggc gcaggctggt ttcgagagac
    22021 tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga atttgccgtt gaccgggaag
    22081 gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac ccgcgtcaat caacaccagg
    22141 tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca gaatgcagcc gttttcaccg
    22201 ctgcctacaa tggtgttata ggaaggatag cgcgcaccgt ggcggttaaa ttcgtggtga
    22261 atttcgcctt ccagatggta ctcgaacatt cccggacggc atttttccat cgcccgtgta
    22321 tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg caatctcttc tggcgatttg
    22381 aacaggcgca tttcatgaac aacaggacgc cagtcgatca tcgttgccgg tgcggtgaga
    22441 ttttgccgcg aacctttacg cagtttttcc agcgcactgt tcacgatttc atcagcatat
    22501 gcatattcgc cctgggcatg gtaaaccaca tccaggccgt taagtagttg ataaagttgc
    22561 tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca gtttctctgg cgcggcatcc
    22621 tggcctaagc gacggccaaa ccagatctcc gccgtcaggt cgcgaacgcg gttaaacaga
    22681 acgctgtggt tatgagtgtc atcgctttta atcagcacca gcaccgcttc cggttcgtta
    22741 aagccggtga agtaccagaa gtcactgttc tgacgatagg ggtattcgct gtcggcgcta
    22801 cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc cgggttgcat ttgctccacc
    22861 agggcctgac ggcgacgctg aaactcttgc cgggatatct cactcataac actctcctta
    22921 cgttttttgt ttttagtgta gagtcggttt ttgtacttct ggcgcggtcg gttgcggatg
    22981 agtaaaggtg tcgtggcata acagcgcggc aacacgaacg tattcgatga tctcttcaag
    23041 cgacatttca agctcttcct gatcttcgtc ttcgtcgtag cccagctgcg cgatgttacg
    23101 cagatcgtcg atggcttcac cggtttcgcc ggtcactttg tccagcttcg gttgcgtaac
    23161 gccaagacca agcaggaagt gattgaccca acctgccagt gcatcagccc gatcgaaaac
    23221 gctgacatca tcgccatcag gcagataaag ctgaaaaagg aagccgtcat cctgcagggc
    23281 atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc agctcatgac cgaaagccat
    23341 gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag ctgtcatcgt taccgccaca
    23401 tatcatccca ctgattaaac catgcatctc ggcaggggtc agacccgtcc cttgttggtt
    23461 cagatactgg ttcatttcgt tgtaaccagg catttcgttc tgtatagaca taagcattcg
    23521 tcgtcaaagg gaggaatatt catgatatgc taccactttg ggccctggtg gaccagaaaa
    23581 gggcttgtct cttctcatca gggtagctat agcgtcgccc cttcgcagac catgggtcta
    23641 aagacgaagg cagcgcagtc aatcagcagg aaggtggcat gtctgcacaa cccgtcgata
    23701 tccaaatttt tggccgttca ctgcgtgtga actgcccgcc tgaccaaagg gatgcgttga
    23761 atcaggcagc ggacgatctg aaccaacggt tgcaagatct gaaagaacgc actagagtca
    23821 caaatactga acagttggtc ttcattgccg cattgaatat cagctatgag ttagcgcaag
    23881 aaaaagcaaa gactcgtgac tacgcggcaa gtatggaaca gcgtattcgg atgctgcagc
    23941 agaccataga acaagcgtta cttgaacaag gtcgcatcac cgaaaaaact aaccaaaact
    24001 ttgaatgaca cttttcggtt tactgtggta gagtaaccgt gaagacaaaa tttctctgag
    24061 atgttcgcaa gcgggccagt cccctgagcc gatatttcat accacaagaa tgtggcgctc
    24121 cgcggttggt gagcatgctc ggttcgtccg agaagcctta aaactgcgac gacacattca
    24181 ccttgaacca agggttcaag ggttacagcc tgcggcggca tctcggagat tcccttctta
    24241 tctggcacca gccatgacgc aactaccaga gctcccactg acattatccc gacaagaaat
    24301 ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg gaacaacagc tggaaatggg
    24361 tcaacaagcc gctacccgga tgatgactta tcccccggtg gtgatggcgc atacggtcgc
    24421 tgtattcctc tcttttgatg gtgaactcga cacccagccg ctaatagaac aactctggcg
    24481 cgccggtaag cgcgtatatc ttccagtttt gcatcccttt agtgccggta atttgctgtt
    24541 cctgaattac catccgcaaa gcgaactggt gatgaacagg ttgaagatcc atgagccaaa
    24601 actggatgtg cgtgacgtgc tacccctttc ccgattagac gtgctgatca caccgctggt
    24661 cgcctttgat gagtacggtc agcgcctggg aatgggcggt ggtttttatg atcggacctt
    24721 acaaaactgg cagcactata aaacgcaacc ggtgggttat gcgcatgatt gtcagttggt
    24781 ggaaaaactc cccgttgaag agtgggatat ccctcttcct gcggtggtta caccgtcgaa
    24841 agtctgggag tggtaagggc gatacacctg catcgccctg attgacatcg ttgattcttt
    24901 aacctaattt agtgagtaag ggtaagggag gattgctcct cccctgtgac tgttaataag
    24961 ctgggaaact tatgagtaac agtacaatca gtatgatggc aagtcgcatc ataacccttc
    25021 tccttcaagc cctcgcttcg gtgagggctt taccgttaca gccccatgct gcccagctat
    25081 cgtaagctct ctttaaataa ccacaacgca ttgatctacc tttaaatctt ttcttcgagc
    25141 gtgctcgaaa aaccccatgt aaaacagaga taatttttgg gaaaaaggat ctattacggc
    25201 agtaaacgtg ggcgatgaag tggctatcaa aaagttcggt tcagggcgat gcaccccgca
    25261 tcgccctgat tgacatcgtt gattctttga cctaatttag tgagtaaggg taagggagga
    25321 tgactcctcc cttgagactg actgttaata agctccgaaa cttatcagta acagtacaat
    25381 cagtatgatg acaagtcgca tcataaccct tctcctttaa gccctcgctt cggtgagggc
    25441 tttaccgtta cagccccatg ctgtcccgcc atcgtaaatc cccattaaat aaacacaacg
    25501 cattgatctg actttgattt attttctgga gcagactcgc aaagtagaat gcgcaacgcg
    25561 gcaacggtgt ggagaaggga taaaaaaacg ggcaagtcag tgacctgccc gttaattttc
    25621 agagaagggg aattagtaca gcagacgggc gcgaatggta cccggaatag ctttcattgc
    25681 ctgcagcgct ttttcggcaa cgtcttcgtc agcttcaata tcaataacta cataacccat
    25741 ctgggcggaa gtttgtagat attgcgcggc gatgttgacg ccctgctcgg cgaagatttt
    25801 gttcagcgca gttagcacgc ccggacggtt ttcgtggatg tgcatcagac gacgaccacc
    25861 atgcagtggc agcgagactt ccgggaagtt caccgcagag agcgttgagc cattgtcaga
    25921 atacttgatc aatttacccg caatttccaa gccgatattc tcctgcgctt cctgagtcga
    25981 accgccaatg tgtggcgtca gaaggacgtt gtcgaattca cacagtggag aggtaaatgg
    26041 atcgctattg gtcgccggtt ccgtcgggaa tacgtcgatt gccgcccccg ccagatgttt
    26101 gctcgccagc gcatcacaca gcgccggaat atccaccaca gtaccgcgcg aggcattaat
    26161 cagcagcgag ccgggcttca ttagcgaaat ctctttcgcg cccatcatat ttttggtgga
    26221 cggattctct ggtacatgca gactcaccac atcgctcata ttcagcaggt cagaaagatg
    26281 ctgtacctga gtggcgttgc ccagcggcag tttgttttca atatcataaa agtaaacata
    26341 cattcccagc gattcagcca gaatgcccaa ttgcgtacca atatgaccgt agccgatgat
    26401 acccagcttt ttgccgcgcg cttcaaaaga acccgccgcc agtttgttcc acacgccacg
    26461 gtgcgcttta gcattggctt ccggcacgcc gcgcaatagc agcagcagtt cgccaatcac
    26521 cagctccgca acagagcgcg tatttgagaa cggtgcgtta aataccggga tcccgcgctt
    26581 tgccgccgca tccagatcaa cctggtttgt tccgatacag aaacagccaa tagcgaccag
    26641 tttttctgcg gcgttgatca cgtcttcagt cagatgggta cgggatcgca ggccgatgaa
    26701 gtgggcatcg cggatggatt cttttaattg ttcatcatcc agcgcgcctt tgtgaaattc
    26761 gatgttggtg taaccagctg cacgaaggct ttccagcgcc ttttggtgca cgccttctac
    26821 cagcagaaac ttaatcttgt ctttctccag cgataccttt gccatttacc caatcctgtc
    26881 ttttgaaatg ttgtgtgcgg atttgcatcc gcctttcaac atatcaaaaa ataatattgc
    26941 ggcaatatga acgtttgcgt cgcgatgttg aggaaatatc acgcaaaggt aattcttgga
    27001 tgaatatgct ggtttagagg atttatttag agcaatcgac aattgcctgg taaaagcgtg
    27061 acacatgtca ccaaatttaa tgaagagaat ttttttaacg ggggaggttc ccccgtcaga
    27121 tcatttcaca atggttttga caccgtcagg tgtgccaatc agcgcaacgt ccgcgccacg
    27181 gttagcaaac aagccaacag tcaccacgcc aggaatcgca tttatggcgt tttccatcgc
    27241 tatcgggtca aggatttcca tgccgtggac gtcgaggatc acgttgccat tatcggtcac
    27301 cacgccctga cggtattccg gacgaccgcc cagtttcacc agctggcgcg ccactgcact
    27361 acgtgccatc gggataactt ctactggcag cgggaattta cccagaatat caacctgctt
    27421 ggaggcgtcg gcaatacaga taaatttttc cgcaaccgaa gcaatgattt tttcacgggt
    27481 cagcgccgcg ccgccgcctt tgatcatttg catgtggccg ttgatttcat ctgcgccatc
    27541 aacgtagatg ccaaggctgt cgacttcgtt gagatcaaaa acgtgaatgc cgaggctttt
    27601 cagtttttca gtggaagcat ctgaactgga aacggcccct tcaatctggc ctttcatcgt
    27661 gccgagcgcg tcaataaagt gtgcggcggt ggaacctgta cctacaccaa caatggtacc
    27721 gggctgaaca tactgaagtg ccgcccatcc tactgctttt ttcaattcat cctgcgtcat
    27781 gatcgtttcg cctgtggtat gaaatttcac acgcattata tacaaaaaaa gcgattcaga
    27841 ccccgttggc aagccgggtg gttaactcat ccataaaata tcgcgcaatg gcgggcatcc
    27901 cctttcgccc cgcaaataaa gcatacaacg gtctgggtat gccgctccac ggtgcgaaca
    27961 gaggcaataa cgccacgcca ttcaacataa ccagggcgcg aagtcgctaa gcgaaatgca
    28021 atagcgcaaa tccctgatct tttaagctgc gcgtttaacc attgatacaa atgttacccg
    28081 ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag gaaaaagcat
    28141 ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac aggcactgga
    28201 tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca ttacacaatc
    28261 agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc tgttggtgcg
    28321 taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc tgcgccaggt
    28381 ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc cgctgctgct
    28441 ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac tggctcctgt
    28501 gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc gtactcagga
    28561 acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg cgctgccgag
    28621 ttgtcttgtc gataaactcg gtgcgctcga ctatctgttc gtcagctcaa aaccgtttgc
    28681 cgaaaaatat tttcctaacg gcgtaacgcg ttcggcatta ttgaaagcgc cagtggtcgc
    28741 gtttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg atcttcctcc
    28801 tggcagcgta ccctgccata tcgttaattc ttcagaagcg ttcgtacaac ttgctcgcca
    28861 gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagagctgg ccagcggtga
    28921 actgattgac ttaacgcctg ggctatttca acgacggatg ctctactggc accgctttgc
    28981 tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgtta ctcgattatg gtcacaaagt
    29041 ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc gttgtcctca
    29101 cttctttttg tattccttga atcacatcac aaaatagaca a
//
LOCUS       DADLTI010000035        28750 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLTI010000035 DADLTI010000000
VERSION     DADLTI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.035"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            353..1711
                     /locus_tag="JN755_001408"
     CDS             353..1711
                     /locus_tag="JN755_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAY7380258.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="JN755_001409"
     CDS             complement(1950..3335)
                     /locus_tag="JN755_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7380259.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDMDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JN755_001410"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JN755_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7380260.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="JN755_001411"
     CDS             complement(3729..4109)
                     /locus_tag="JN755_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY7380261.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="JN755_001412"
     CDS             complement(4464..4898)
                     /locus_tag="JN755_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380262.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="JN755_001413"
     CDS             complement(4886..5287)
                     /locus_tag="JN755_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380263.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="JN755_001414"
     CDS             complement(5547..6116)
                     /locus_tag="JN755_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7380264.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7037)
                     /locus_tag="JN755_001415"
     CDS             complement(6861..7037)
                     /locus_tag="JN755_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAY7380265.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            complement(7338..7604)
                     /locus_tag="JN755_001416"
     CDS             complement(7338..7604)
                     /locus_tag="JN755_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380266.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="JN755_001417"
     CDS             7745..7951
                     /locus_tag="JN755_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7380267.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="JN755_001418"
     CDS             8046..8648
                     /locus_tag="JN755_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380268.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..10765
                     /locus_tag="JN755_001419"
     CDS             9848..10765
                     /locus_tag="JN755_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380269.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="JN755_001420"
     CDS             10850..11722
                     /locus_tag="JN755_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY7380270.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12094..14940
                     /gene="ag43"
                     /locus_tag="JN755_001421"
     CDS             12094..14940
                     /gene="ag43"
                     /locus_tag="JN755_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAY7380271.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            15011..15169
                     /locus_tag="JN755_001422"
     CDS             15011..15169
                     /locus_tag="JN755_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY7380272.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            15324..16142
                     /locus_tag="JN755_001423"
     CDS             15324..16142
                     /locus_tag="JN755_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7380273.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            16484..16957
                     /locus_tag="JN755_001424"
     CDS             16484..16957
                     /locus_tag="JN755_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7380274.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            16973..17449
                     /locus_tag="JN755_001425"
     CDS             16973..17449
                     /locus_tag="JN755_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY7380275.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            17512..17733
                     /locus_tag="JN755_001426"
     CDS             17512..17733
                     /locus_tag="JN755_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY7380276.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17896..18270
                     /locus_tag="JN755_001427"
     CDS             17896..18270
                     /locus_tag="JN755_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY7380277.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            18317..18691
                     /locus_tag="JN755_001428"
     CDS             18317..18691
                     /locus_tag="JN755_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY7380278.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18688..19179
                     /locus_tag="JN755_001429"
     CDS             18688..19179
                     /locus_tag="JN755_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380279.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            19191..19388
                     /locus_tag="JN755_001430"
     CDS             19191..19388
                     /locus_tag="JN755_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY7380280.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            19473..20033
                     /locus_tag="JN755_001431"
                     /pseudo
     CDS             19473..20033
                     /locus_tag="JN755_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            20105..21109
                     /locus_tag="JN755_001432"
     CDS             20105..21109
                     /locus_tag="JN755_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY7380281.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            21391..21672
                     /locus_tag="JN755_001433"
                     /pseudo
     CDS             21391..21672
                     /locus_tag="JN755_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(21822..21909)
                     /locus_tag="JN755_001434"
     tRNA            complement(21822..21909)
                     /locus_tag="JN755_001434"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Ser,seq:gga)
     gene            22143..23081
                     /gene="ghrA"
                     /locus_tag="JN755_001435"
                     /pseudo
     CDS             22143..23081
                     /gene="ghrA"
                     /locus_tag="JN755_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            23136..23873
                     /locus_tag="JN755_001436"
     CDS             23136..23873
                     /locus_tag="JN755_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7380282.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            23897..24451
                     /locus_tag="JN755_001437"
     CDS             23897..24451
                     /locus_tag="JN755_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7380283.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            24553..25044
                     /locus_tag="JN755_001438"
     CDS             24553..25044
                     /locus_tag="JN755_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7380284.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(25108..25883)
                     /locus_tag="JN755_001439"
                     /pseudo
     CDS             complement(25108..25883)
                     /locus_tag="JN755_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(25910..26326)
                     /gene="csgF"
                     /locus_tag="JN755_001440"
     CDS             complement(25910..26326)
                     /gene="csgF"
                     /locus_tag="JN755_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY7380285.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            complement(26351..26740)
                     /gene="csgE"
                     /locus_tag="JN755_001441"
     CDS             complement(26351..26740)
                     /gene="csgE"
                     /locus_tag="JN755_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY7380286.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(26745..27395)
                     /gene="csgD"
                     /locus_tag="JN755_001442"
     CDS             complement(26745..27395)
                     /gene="csgD"
                     /locus_tag="JN755_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7380287.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            28149..28604
                     /gene="csgB"
                     /locus_tag="JN755_001443"
     CDS             28149..28604
                     /gene="csgB"
                     /locus_tag="JN755_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY7380288.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            28645..>28750
                     /locus_tag="JN755_001444"
     CDS             28645..>28750
                     /locus_tag="JN755_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY7380289.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         7121 a         7257 c         7338 g         7034 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccatgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgagctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt
    11701 atggaactgg attttcttct gaacagtctg ccagggaaat gcctgataac attacgttaa
    11761 ccaactgaaa acatgacaca aacccctcat actcctcctg tttctcttgc gtgaccgcct
    11821 tgtcgttaat agaataaaac tatcgataaa acagatatcg atcgttgcta tcgatcgttt
    11881 atagtggtta atggtctgcg aaaaggcagc tgaatctctt catcatgcag aacggaattg
    11941 cacacaacgg actgatactt ctctggcgga tgagagaggg gaagcattta tgtccgggaa
    12001 aaaccacttt atctgaccat gctgttttgt acctgccggt atccactttt gtgggtaccg
    12061 gcttttttat tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac
    12121 aggctggtat ggaatcacat tacgggcacc ctggtggtgg cttccgaact ggcccgctca
    12181 cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac atcagtcccg
    12241 gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg aacactgaca
    12301 aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag tagcggtctg
    12361 gagtatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa tggcggtatc
    12421 gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg aggaagcgtt
    12481 tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc agtgaatacc
    12541 actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg taccgtcatt
    12601 aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac ggttgtgaat
    12661 accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt tgttcgcgga
    12721 aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc tgaaggaaca
    12781 gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca tgcgctggat
    12841 accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc tggcactgtt
    12901 gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt caccaccgtt
    12961 aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt caccctgaaa
    13021 cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa ccgtctgggc
    13081 aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg cggtcgcctg
    13141 gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg aaccctggca
    13201 gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc cctgattgca
    13261 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
    13321 tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt taatgccgga
    13381 ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
    13441 agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa tggcgatgtg
    13501 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
    13561 gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt ccataaactg
    13621 accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg ccttgatggc
    13681 agcaatacct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
    13741 gcgtttacaa atgtcggaaa cagtaacctc ggggtggcaa cctccggaca gggtatccgg
    13801 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
    13861 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
    13921 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgct gacacaggca
    13981 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
    14041 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
    14101 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
    14161 gacctgctga gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgccgca
    14221 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
    14281 gatgccggca gcctgggcgg atacctgaat ctgacacaca cgtcctccgg cctgtgggct
    14341 gacattgtgg cacagggaac ccgccacagc atgaaagcgt catcggacaa taacgacttc
    14401 cgtgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
    14461 gacaacctga tgctggagcc acaactgcac tatacctggc agggacttac cctggatgac
    14521 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
    14581 ggtttccgtc tgggcagcca caacgatatg agctttggtg aaggcacctc atcccgtgac
    14641 actctgcgcg acagtacaaa acaccgtgtg agtgaactgc cggtgaactg gtgggtacag
    14701 ccttctgtta tccgcacttt cagctcccgg ggtgacatga gcatggggac agccgccgcc
    14761 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
    14821 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
    14881 gtcagcggca gcagcgctga aggttataac ggtcaggcca cgctgaatgt gactttttga
    14941 taatcaggtc actctccttc aggccccggt catcatgacc ggggcttttt actttcaggg
    15001 agcaacagat atgccgggtt gtacatcacg tttattgccc gaaggccctt tctcacgcga
    15061 acaggctgtt gccgtcaaaa cagcttaccg caatgtgttt actgaagatg accagggaac
    15121 atattcccgg ctggttatcc gtaatgctga aggtcagttg cgctggtgat gccggaacac
    15181 agcttaatac tcagctcgcc agttaagggc ttctcagaca ataaacgtca ttcatttcat
    15241 ctgtcaggcc gcgtcttctc cgggagacgc agctttttca tttattacac cagtaaatcg
    15301 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
    15361 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgttccc agtattttcg
    15421 gtgaagaccg gcatacttcc cgcagtaaac gatatgcgta cattcccacc atcaccgtac
    15481 tggaaagcct gcagcaggaa ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg
    15541 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
    15601 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
    15661 agatgctgcc gggttacttc agattcgtct gccagaatgg ctgcgtctgc ggtcagtctc
    15721 tgggggaagt gcgtgttcca caccggggaa atgtggtgga gaaagttatc gaaggggctt
    15781 acgaagtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg cagtcgctgg
    15841 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
    15901 acgaacatca gccagtcacc accgctgaca ttctgacacc acgacgcagg gaggattacg
    15961 gtaaagacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
    16021 ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac atcgacaccg
    16081 atattaagct caaccgcgca ttgtgggtaa tggcagaaac gctgctggag agcctgcgct
    16141 gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg ccccggaggc
    16201 gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta cccgtctgct
    16261 ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg catctggcca
    16321 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    16381 catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc cagctcccga
    16441 cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa cattgtctca
    16501 gaacaccacc gcttcggcct gtgcaccgga gactggcctg caacaactgg ttgccactcc
    16561 cgtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg
    16621 ggtcaccctg gaaccccgtg tcttcggctg gatggaccgt ctgtgtgaag actactgcgg
    16681 tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga
    16741 tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc gcgctgaaat
    16801 gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg
    16861 tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg ccctgcagca
    16921 tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga caatgcagca
    16981 gctttccttt ctgcccggaa acatgacgcc cggcgagcgc agtctcattc tgcgggccct
    17041 gcaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc
    17101 acgggaatgg ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta
    17161 tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg
    17221 tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt
    17281 ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat
    17341 caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat
    17401 agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac ccgtcacaac
    17461 cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacat catgagaatc
    17521 atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg tctttttccg
    17581 ttctgtaccg gtaagtatcg ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg
    17641 caggatattc ccggcgttct tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg
    17701 tatgtccggc tgatgagtgt caccctgaac tgatgttccg tcacccggga aacgagtacg
    17761 accagatgac ctcagacgcc gctgtggatg aggccattct catcaatgca tacatcttca
    17821 ccgaaaccgg tcagcgcgcc ggctgacctg tcatcgttaa accattctta accacttttc
    17881 agagaggatt ttatcgtgtc agacaaactc tccgggataa ctcatcccga tgacaatcac
    17941 gaccgcccct ggtgggggct tccctgcaca gtgaggccct gttttggcgc tcgtctggtg
    18001 caagagggta accgcctgta ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc
    18061 gacgcagatg cataccatct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
    18121 atgctcacca gcggtgaact gaatccccga tatcagcata ccgtcacgct gtatgcaaaa
    18181 gggctgacct gcgaagccga caccctcggc tcctgtggtt acgtttatct ggctgtttat
    18241 ccgacaccag cacccgcaac cacctcataa ccccgaagct ctcaccttta gcactaccag
    18301 ttacagagaa cttaagatga aaacattatc cgacacacat gtacgggagg tatctcgctg
    18361 cccgtctccc gtcaccatct ggcagacact gctcatccga ctgctggacc agcattatgg
    18421 cctcacactg aatgacacac cgtttgtcga tgaacgtgtg attgagcagc atattgaggc
    18481 aggcatttca ctgtgtgatg cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac
    18541 cgaccagccg ggattcagca cctgtccccg ctctcagtta ataaacagta ttgatatcct
    18601 ccgggctcgc cgggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
    18661 taccctgggt aagtatccgg aggcgaaatg atgaaactgg ccctgacact ggaagccgac
    18721 agcgttaacg tacaggcact gaacataggg cgcattgtcg ttgacgtcga tggtattgag
    18781 ctcgctgaac tgattaacat ggtctgcgat aacggctact cccttcgtgt tgttgatgaa
    18841 tctgaccaga cctcagcaga atgcacgcca ccatttgcta cccttaccgg catacgctgc
    18901 agtaccgcac atatcacgga aacggacaac gcctggttgt actcgctgtc acaccagacc
    18961 agtgacttcg gtgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc
    19021 gatgcgtggt catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    19081 cgtctggtcg tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccagc
    19141 gctgaatgcc tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat
    19201 taaccacgga aacagcgctg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    19261 gatcgggaat tatctttgac aacgatgagg ataaaacgga ttcagcagca ctgttgccct
    19321 gtatcgaaca ggcccgggag gatgtccgta ccctgcgtca tctgcagctt ctgcaccaga
    19381 accggtgaag ctcactcacc agacttcatt cctctgacgc cagcctgcac agggctggcg
    19441 ttttcattta tctgcaaaaa ggaatatcga ttatgtcaga tatcacaatc tcccgtccgg
    19501 aagtggtcaa cgggcatacg gacgttatct gctcaaccag cattcgccac attctggctg
    19561 tacgaaagag tacactgttg caaatcgaca cacttatccg acaactggct gaaatctcgg
    19621 tgctgacgga aagcatcgga ggtaaaactg ccccggactg ggccatgaaa caggattttc
    19681 gctgcggctg ctggctgatg gagaaacccg aaacagcaat gaaagccatt acccggaatc
    19741 ttgatcgcga aatctggcgt gacctgatgc aacgttccgg gatgctgtca ttaatggata
    19801 cgcaggcccg tgatacatgg taccggtcac tggagtacga taattttccg gaaatcagcg
    19861 aagcgaacat tctgagcaca tttgaacaac tgcaccagaa taaggatgag gtgtttgagc
    19921 gaggggtgat caacgtcttc agagggctga actggaatta caaaaccaat tgcccctgta
    19981 aatttggcag taaaattatc gtcaacaatc tggtgagatg ggaccggtgg ggataatgat
    20041 atggtcaccc ccaccttaac agcagtacgc tgttaaggta aatcccttta acggagagac
    20101 catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct tccacattca
    20161 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta ctaaactgat
    20221 gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg cgggtgcaca
    20281 ctttatggct cgctgtatct ctgattttgg gcatgaaact aagctgattt ctccacagtt
    20341 cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag ccatatgcga
    20401 agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagaccc agcaggccat
    20461 gagggcgtta caccgggtca gagaatcgct catcagagat aaggtgaaaa caaccaacca
    20521 gatccacggt ttcttgctgg aatttggaat cagcctgccg ataggtgatg ctgtaataaa
    20581 acggttgtct ctcgtgttgg ctgaacacga aatccccgaa tatctgagcc gccttttggt
    20641 gaggttgcat acccactact tttaccttgt tgagcaaata gcagagctgg aatcagaatt
    20701 aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc ctggtgttgg
    20761 tccgattact gccagcttac tatcgtcgca acttggtgac gggaaacagt tctcatgcag
    20821 cagggatttt gcagcttcca caggacttgt tccccggcag tatagcactg gaggaaaatc
    20881 gactctgttg ggtatcagta agcgaggtga taaaaattta cgccggttac tggttcagtg
    20941 tgcccgctca ttcataatgc gacttgaacg caagcatgga aaacttgctg agtgggttcg
    21001 ggagcaactc aacaagaagc attcgaatgt ggttacctgc gctctggcaa ataaactggc
    21061 tcgaattgcc tgggcaatca ctactcagaa aaaagaatat caggcctgat atagatgctg
    21121 atataactca attagtttat tttaaacaat aagatacaag ttacctttct ggttttgcga
    21181 agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acagatgaaa
    21241 tggctcactg aagccgaggg gatttttagg attatcaggt gcggaactca tcggggcgcg
    21301 ggcatccttg cccacagaga cgccggatag atttaagcaa gccaaccatt aatcaaaaat
    21361 cagtgttgca aaaacggggg tgaccataga tttcatctta tcaccggaca acaggctgac
    21421 cgactcgccg atctggaaag aatgttgcac ctgttcagcg gtaaaccaat ccccgacaac
    21481 cggggaaaca tcacccttcg tctggatgat cacattcagt ctgttcaggg taaagagagc
    21541 tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcgca tatcacgttc
    21601 agaaaaccag aactggttga cagactgaat gatattatcg caaaacacta tcctgatatg
    21661 ctggcagtat agctttgcca gtatgctcaa agatcggaaa ttgtccgata agatatgatc
    21721 gcggggcata taaaccggtt tcagatggca tacccgatgg catactgtct ggaaggaaaa
    21781 ataaaaaagc gcatgttaaa caataacatg cacttataat ctggcggtga gggggggatt
    21841 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    21901 cacctcacca aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg
    21961 acctgaggtc aacaaacttt ttgaaagaat cgcgcgttta ttcaaacttc aatcaatgtg
    22021 tggttttaat aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg
    22081 gcctttgtag atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca
    22141 gtatggatat catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc
    22201 gcaaagctat tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg
    22261 attatgtttt agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg
    22321 tgttcgcact cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa
    22381 tgctgaaccc ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc
    22441 aggaatatgc tgtcagtcag gtgctgcatt ggtttcgacg ttttgatgat tatcgcatcc
    22501 agcaaaatag ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg
    22561 gcattttggg cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct
    22621 ttccgctgcg ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg
    22681 gacgggaaga actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga
    22741 atacccctga aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg
    22801 cgtatctcct caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc
    22861 tggatagcgg gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc
    22921 ctgaaagtcc gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta
    22981 cccgtcccgc tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg
    23041 agagggtctg cgggcaagtc gaccgcgcac gcggctacta ataaagcatc aggattcctg
    23101 ctatccttgg cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc
    23161 ataccgttgc cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac
    23221 aaaagggcat taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc
    23281 atcactggca cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc
    23341 tgcgcggcat tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa
    23401 tgtttgactc gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg
    23461 acaaagcgac caatacacaa gcgatgatcg ccactatcgc cagcggcaat gtgcatataa
    23521 taagccatcc cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg
    23581 cgaaacatca ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca
    23641 gtgaagacaa ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat
    23701 taggctcgga ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc
    23761 tcgacgcggt agattttccg ccagagcgca ttttgaatgt ttctccgcgc cgcttactga
    23821 acttccttga atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag
    23881 taatggaaat atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta
    23941 ttaccgccaa ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa
    24001 actggctgcg aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg
    24061 tgatatgacg caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt
    24121 gccgccatat cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct
    24181 ttccgaacga gggatgccac tggcggatac gccagccgat cacatcggca cattgctgct
    24241 tgcggcttcc tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact
    24301 gttcagtgag tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc
    24361 aaccacgcct ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg
    24421 ggacgagctg gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac
    24481 agataatttg ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct
    24541 atcatgcgcg gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt
    24601 atctggggat gggtggctgt ttccctgggt ttactaagct gggcgggctt cctgggctgt
    24661 acggcctact ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc
    24721 ctgcttagtg gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat
    24781 ctggaaattc tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc
    24841 aaacagctgc tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc
    24901 gggcagggtg actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac
    24961 gcaatgaaaa acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag
    25021 caggaaatca aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg
    25081 gattattaat tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta
    25141 tttcaccaga atgtcattct gccgttctgc tttattttgc aaatcccaca gaccacggtc
    25201 gataccatca ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat
    25261 aacgggttcg tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa
    25321 gcggaaaacc ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga
    25381 gaggatctca ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg
    25441 gtattgcgtg tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt
    25501 gctttcataa ccaataatcg aaccttcaac cataatattt gccgccgtta aagattgcag
    25561 cgggattcgg ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg
    25621 ctcgttaagc aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt
    25681 gccgggtagg gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc
    25741 ttacccgtcg gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat
    25801 gtcggtctgg cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca
    25861 accaaaagaa ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg
    25921 ttgagttatt ttgtaaaacc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg
    25981 tcacgttcaa ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca
    26041 ccatgcggcc cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact
    26101 ggatggcctg agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat
    26161 agctcggatc tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat
    26221 ttgggttacc accaaagttt ggattacgga actggaaagt catggttcca gcccaactta
    26281 atggcgaaat aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac
    26341 aattttttat ttagaattca tcatgcgcca aatcgcccgt acttaataac gcctgattta
    26401 tctggcgacg atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcga
    26461 agtctctttt caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg
    26521 tgatccagct tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt
    26581 cactttccca tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat
    26641 ggtcagttaa caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca
    26701 gaaattctgc cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta
    26761 tcgtttgccc aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta
    26821 taaagatgcg ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta
    26881 gacgcgccga tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat
    26941 tccgtgctgt tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc
    27001 gtaaagtagc attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc
    27061 tccatggcat aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa
    27121 tcttcaggcg tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc
    27181 tgccaataat ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct
    27241 gaagagatat cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat
    27301 tgtttaaggt gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat
    27361 gtatgaccat gaatactatg gacttcatta aacatgatga aaccccgctt ttttattgat
    27421 cgcacacctg acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact
    27481 gctgtgtgta gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc
    27541 tgatttcaat ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtaa
    27601 ctcattatat taaaatgtta acattaaggt tttattaagt ttagaaatga tagaaaagtt
    27661 gtacatttgg tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa
    27721 taatacatat aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt
    27781 tctccacacc tccgtggaca atttttttac tgcaaaaaga cgaggtttgt cacggcttgt
    27841 gcgcaagaca tatcgcagca atcagcgacg ggcaagaaga atgactgtct ggtgattttt
    27901 gatagcggaa aacggagatt taagagaaaa caaaatattt tttgcgtaga taacagcgta
    27961 tttacgcggg ttttaatact ttggtatgaa caaaaaaaga aaaatacaac gcgcgggtga
    28021 gttattaaaa atatttccgc agacatactt tccatcgtaa cgcagcgtta acaaaataca
    28081 ggttgcgtta acaaccaagt tgaaatgatt taatttctta aatgtacgac caggtccagg
    28141 gtgacaacat gaaaaacaaa ttgttattta tgatgttaac aatactgggt gcgcctggga
    28201 ttgcagccgc agcaggttat gatttagcta attcagaata taacttcgcg gtaaatgaat
    28261 tgagtaagtc ttcatttaat caggcagcca taattggtca agctgggact aataatagtg
    28321 ctcagttacg gcagggaggc tcaaaacttt tggcggttgt tgcgcaagaa ggtagtagca
    28381 atcgggcaaa gattgaccag acaggagatt ataaccttgc atatattgat caggcgggca
    28441 gtgccaacga tgccagtatt tcgcaaggtg cttatggtaa tactgcgatg attatccaga
    28501 aaggttctgg taataaagca aatattacac agtatggtac tcaaaaaacg gcaattgtag
    28561 tgcagagaca gtcgcaaatg gcaattcgcg tgacacaacg ttaattacca ttcgactttt
    28621 aaatcaatcc gatgggggtt ttacatgaaa cttttaaaag tagcagcaat tgcagcaatc
    28681 gtattctccg gtagcgctct ggcaggtgtt gttcctcagt acggcggcgg cggtaaccac
    28741 ggtggtggcg
//
LOCUS       DADLTI010000036        28576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLTI010000036 DADLTI010000000
VERSION     DADLTI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.036"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1181
                     /locus_tag="JN755_001445"
     CDS             <1..1181
                     /locus_tag="JN755_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380290.1"
                     /translation="ASTTKARTSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLT
                     FMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAV
                     LLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFGSSQGPLLAAVIIAP
                     YGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIINPLPRNKVVLAVSIL
                     LILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKI
                     GRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFSAILVYAQELLPGRI
                     GMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD
                     "
     gene            1419..3095
                     /locus_tag="JN755_001446"
     CDS             1419..3095
                     /locus_tag="JN755_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7380291.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(3228..4532)
                     /locus_tag="JN755_001447"
     CDS             complement(3228..4532)
                     /locus_tag="JN755_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7380292.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            4684..5644
                     /gene="aes"
                     /locus_tag="JN755_001448"
                     /pseudo
     CDS             4684..5644
                     /gene="aes"
                     /locus_tag="JN755_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(5641..6603)
                     /gene="hemH"
                     /locus_tag="JN755_001449"
     CDS             complement(5641..6603)
                     /gene="hemH"
                     /locus_tag="JN755_001449"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7380293.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(6735..7379)
                     /gene="adk"
                     /locus_tag="JN755_001450"
     CDS             complement(6735..7379)
                     /gene="adk"
                     /locus_tag="JN755_001450"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7380294.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(7560..9434)
                     /gene="htpG"
                     /locus_tag="JN755_001451"
     CDS             complement(7560..9434)
                     /gene="htpG"
                     /locus_tag="JN755_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7380295.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(9544..10149)
                     /gene="recR"
                     /locus_tag="JN755_001452"
     CDS             complement(9544..10149)
                     /gene="recR"
                     /locus_tag="JN755_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7380296.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(10149..10478)
                     /locus_tag="JN755_001453"
     CDS             complement(10149..10478)
                     /locus_tag="JN755_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7380297.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(10531..12456)
                     /gene="dnaX"
                     /locus_tag="JN755_001454"
     CDS             complement(10531..12456)
                     /gene="dnaX"
                     /locus_tag="JN755_001454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7380298.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            complement(12585..13136)
                     /gene="apt"
                     /locus_tag="JN755_001455"
     CDS             complement(12585..13136)
                     /gene="apt"
                     /locus_tag="JN755_001455"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7380299.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(13289..13666)
                     /locus_tag="JN755_001456"
     CDS             complement(13289..13666)
                     /locus_tag="JN755_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7380300.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            13736..14263
                     /gene="priC"
                     /locus_tag="JN755_001457"
     CDS             13736..14263
                     /gene="priC"
                     /locus_tag="JN755_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7380301.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14277..14438
                     /gene="rsmS"
                     /locus_tag="JN755_001458"
     CDS             14277..14438
                     /gene="rsmS"
                     /locus_tag="JN755_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7380302.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14650..18012)
                     /gene="mscK"
                     /locus_tag="JN755_001459"
     CDS             complement(14650..18012)
                     /gene="mscK"
                     /locus_tag="JN755_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7380303.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(18140..18787)
                     /gene="acrR"
                     /locus_tag="JN755_001460"
     CDS             complement(18140..18787)
                     /gene="acrR"
                     /locus_tag="JN755_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7380304.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            18929..20122
                     /gene="acrA"
                     /locus_tag="JN755_001461"
     CDS             18929..20122
                     /gene="acrA"
                     /locus_tag="JN755_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7380305.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            20145..23294
                     /locus_tag="JN755_001462"
     CDS             20145..23294
                     /locus_tag="JN755_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7380306.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVNVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            23840..24214
                     /gene="tomB"
                     /locus_tag="JN755_001463"
     CDS             23840..24214
                     /gene="tomB"
                     /locus_tag="JN755_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7380307.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            24240..24458
                     /gene="hha"
                     /locus_tag="JN755_001464"
     CDS             24240..24458
                     /gene="hha"
                     /locus_tag="JN755_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7380308.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            24630..25181
                     /gene="maa"
                     /locus_tag="JN755_001465"
     CDS             24630..25181
                     /gene="maa"
                     /locus_tag="JN755_001465"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7380309.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            25297..25767
                     /locus_tag="JN755_001466"
     CDS             25297..25767
                     /locus_tag="JN755_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380310.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            25931..27481
                     /gene="pdeB"
                     /locus_tag="JN755_001467"
     CDS             25931..27481
                     /gene="pdeB"
                     /locus_tag="JN755_001467"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY7380311.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(27523..27876)
                     /locus_tag="JN755_001468"
     CDS             complement(27523..27876)
                     /locus_tag="JN755_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7380312.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(27996..28092)
                     /gene="ffs"
                     /locus_tag="JN755_001469"
     ncRNA           complement(27996..28092)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN755_001469"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            28255..28566
                     /locus_tag="JN755_001470"
     CDS             28255..28566
                     /locus_tag="JN755_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7380313.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT         6680 a         7361 c         7386 g         7149 t
ORIGIN      
        1 ctgcgtcaac gaccaaggcc cgaacatcgt ttggtatttt aggtgctatc agcctctcac
       61 atctgctgaa cgacatgatc caatcgctga ttctggcgat ttatccgctg cttcagtcag
      121 aattttctct gacatttatg cagattggca tgataaccct caccttccag ctcgcctctt
      181 cgctactgca accagtggtc ggctactgga ccgataaata tccgatgccg tggtcgttgc
      241 caattggcat gtgctttacc ttaagtggtc tggtgctgct tgcgctggcg ggcagttttg
      301 gcgcagttct gctggcggcg gcgctggtcg gtaccggttc atcggtcttt catccggaat
      361 cttctcgcgt agcccgtatg gcttccggcg ggcggcatgg cctggcgcaa tctatctttc
      421 aggtcggcgg caactttggc agttcccagg gccccttgct ggcggcggtg attatcgcgc
      481 cttatggcaa aggcaacgtt gcctggtttg tgcttgcggc actgctggcg atcgtggtgt
      541 tggcgcaaat tagccgttgg tactcggcac agcaccgaat gaataaagga aaacccaaag
      601 cgacgataat caatccactg ccgcgcaaca aagtggtact ggcagtcagc attctgttaa
      661 tcctcatttt ctcgaaatat ttctatatgg cgagcatcag cagctattac accttttatc
      721 tgatgcaaaa attcggatta tctatccaga atgcccagct tcatctgttt gccttcctgt
      781 ttgccgttgc ggcaggtacg gtgatcggcg ggcctgtagg ggataaaatt ggacggaaat
      841 atgtgatttg gggctctatc ctcggcgttg cgccgtttac gctgatttta ccctacgccc
      901 gcctgcactg gacgggggtt ttaacggtga ttattggatt tatcctcgct tcggcattct
      961 ctgccattct ggtctacgct caggagctac ttccgggacg tatcggtatg atttctggac
     1021 tctttttcgg ttttgctttc ggcatgggag gtctgggagc ggcagttctg gggcttatcg
     1081 ccgatcacac cagcatcgag ttagtctata aaatctgtgc tttcctgcca ctattgggga
     1141 tgttgaccat attcctgcct gataaccggc ataaagactg atttcattgc agccaaaggc
     1201 aaactttggc tgcatcgttt tacagtcgcc ataagccttt cctctgttaa accgccttct
     1261 gttttccgat ccgtgctttt tttgcagcta attccatttt tctgcaaaat tcaacaatta
     1321 ttcataaaca tcatcaatga aattgtcata aactactacc tgtaattttg gttttcccgg
     1381 ccaaaaatgg acaataacca ccaggaaaag gagacggaat gcatcacgcc accccgctta
     1441 tcaccaccat tgttggcggc cttgtgctcg cctttatcct cggcatgctg gccaataaac
     1501 tacgtatttc tcctctggtg ggatatctgt tagcgggtgt gctggcagga ccattcactc
     1561 cgggctttgt tgccgatacc aagcttgcgc cggaactggc tgaactgggc gttattttgc
     1621 tgatgttcgg cgtcggtttg catttttcgc tcaaggattt gatggcggta aagtccatcg
     1681 ccattcccgg cgcgatcgcc cagatagccg tggcgacgct gctgggtatg gcgctctccg
     1741 ccgtgctggg ctggtcgtta atgaccggta tcgtgttcgg cttatgtctt tccaccgcca
     1801 gtaccgtggt gttactgcgc gcacttgaag aacggcaatt aatagacagt cagcgtggga
     1861 aaatcgccat cggttggttg attgtggaag acctggtgat ggttctgacg ctggtgttgc
     1921 tgcccgcagt ggcaggaatg atggaacagg gcgatgtggg ctttgccact cttgcagtcg
     1981 atatggggat caccatcggc aaggtgatcg catttatcgc cattatgatg ctggtaggtc
     2041 gccgtctggt gccgtggatt atggcacgca gcgcggcaac cggttctcgc gaactgttta
     2101 ccctatcagt gctggcgctg gcgttaggga ttgcctttgg tgcggtagag ctgtttgatg
     2161 tctcctttgc actcggtgcg ttctttgccg ggatggtact gaacgagtct gaattgagtc
     2221 accgtgccgc ccacgatacg ttgccattgc gcgacgcgtt tgcggtgctg ttttttgtct
     2281 cagtcgggat gttgtttgat ccgttaattc tgattcagca accgctggca gtgctggcga
     2341 cgctggcaat tattctgttt ggtaaatcgg tcgcggccct gttccttgtc cgtctgtttg
     2401 gtcactccca acgtacggca ttaaccatcg ccgccagcct ggcgcagatt ggtgagttcg
     2461 cgtttatcct ggcgggactg ggaatggcat tgaatttact gccgcaggcc ggacaaaacc
     2521 tggtactggc aggggcgatc ctgtcgatta tgctcaaccc ggtactgttc gcactactgg
     2581 agaaatatct ggcgaagacc gaaacgctgg aagagcagac gctggaagag gcaatcgaag
     2641 aagagaaaca gatcccagtg gatatttgca accatgcgct attggtgggt tacggtcgtg
     2701 taggcagcct gctgggggag aaattgctcg cctctgatat tccgctggtg gtgattgaga
     2761 cgtcacgaac ccgtgttgat gagctgcgag agcgtggggt ccgcgctgta ttgggcaatg
     2821 cagcgaacga agaaattatg caactggcac atctggaatg tgcaaaatgg ctgatcctga
     2881 cgatccccaa cggttatgaa gcgggtgaga ttgtggcgtc tgcccgcgcg aaaaacccgg
     2941 atattgagat tatcgcccgc gcccattatg acgatgaagt gacgtatatc accgaacgtg
     3001 gcgcgaatca ggtagtgatg ggtgaacgtg aaattgcccg tactatgctg gaactgctgg
     3061 aaacgccacc ggcgggtgaa gtggtgacgg ggtaaacggt ggttgccgga tgtaaaatat
     3121 gaaaaatatg taggcatgat aagacgcgcc agcgtcgcat caggcatcga gagccaaatg
     3181 ccagatgcgg cgtgaacgcc ttatctggcc tacgaaccga cgataactta acgatcccag
     3241 taagactctt ccaggctgtc ttcacgctcc ggcaagccgc gcgttaaacg aggtgaatgc
     3301 tggttcagta cctgatagct cacacggtta gcatatttac acacctgcgc taacgatgaa
     3361 taagttaacc aggtgaattt atgtttgctg gagtttggta cgttgctacg atggtagctg
     3421 ttggcggtaa tgtcatgcag caacgctgcc aatgcgccat cccccgctcc attagtgttc
     3481 atgatttttt ccggcccgcc catgtacggc gcaatgtgcg aatagacacg cagcggattc
     3541 tggcaatcct tgtggcgcat ggcgcggcta aactcatact ggttgaattc cgctatagcg
     3601 cccggcagca gcggatgctg ggttttacgt ttcgcttcgt cttcggtaaa gcccgccata
     3661 tacaagccga ttggcccggc ggtgcacagc accagatcta cccagtccag cgccttgtca
     3721 gatgccaaca acggatcgct ttctccggtc aacgcttcgg cttcatcttc gttcatcgca
     3781 aggatagaaa cgtggtcttt gaggaattgc tgccaccact gcggattctc ggcaatgaca
     3841 aacttggtgc ccagcgtcag caccaccggt acgttatatt tcttcgcgta ctcaatggct
     3901 ttcatggttg cttccggcat gggttcaccc ggcttgcaac gcaccagata tgaggtgaga
     3961 accagtgccg aggctccggc aatcacatct tccggaatgc tttcagcccg cagctggttc
     4021 atgtggcctg gactgatagc aaaggtacgt tccccggact cgccaatcag cgtaaagcaa
     4081 cgaccaatcg gaccatccac gccttgtaga tagttaagat cggtacggct ggaagtgtta
     4141 cacaggtaac gataggcata actgccaatt tcaatattgc tgcacatgac gcccagcagc
     4201 accgaacggt cgtccgcgag caccgagtag ttgtgcatgg tgttaccaat ggtgccaccc
     4261 gcaaactgat gggtaatcag gtttttctgt tttagctcct gataaagcac ttcggctacg
     4321 tcatcctcaa tcaccagtga atgcccggcg cttaatccat aacgctcaat gaattcatca
     4381 tccactttcg cttcaatatc gaccagcgtc tggtcaatac ctacgaccca ggcggcgctg
     4441 gtttcgtttt ctggctggaa ttgctgaagc agcggatcgc gtgcatttac agggaagtaa
     4501 tttttggatt tacgtttacc gggaaatttc atggttgttt ttacgggtgg ttaactgagc
     4561 ggcgaatgtt aacacaatct gacgggagcg cgcgagacgc agccagtttc aatatcagca
     4621 ggcgggatcg cattttcatg gcagtggttc cttacaatga cgtaatttga aaggagtttt
     4681 tgtatgaagc cggaaaacaa actacctgtt ctggacctta tttctgctga aatgaagacc
     4741 gttgtgaata ctcttcagcc ggatttaccg tcctggcccg caacgggaac gattgctgag
     4801 caacgacagt attacacgct tgagcgccga ttctggaatg cgggcgctcc agaaatggca
     4861 accagagctt acatggttcc aacaaaatat gggcaggtgg aaacacgtct cttttgtccg
     4921 cagccagata gcccggcgac gctattttat ttgcatggaa ggcggtttta ttctcggcaa
     4981 tctcgatacc cacgatcgca tcatgcgcct gctggcaagc tacagccaat gtacggtgat
     5041 tggtattgat tacacccttt cacctgaagc gcgttttcca caagcgatag aggaaattgt
     5101 ggctgcttgt tgttatttcc accagcaggc ggaggattat caaatcaata tgtcccgcat
     5161 tggctttgcc ggtgattccg caggtgccat gctggcgctc gccagtgcgt tgtggttgcg
     5221 tgataaacag atcgattgcg gtaaaattgc gggtgttttg ctgtggtatg ggctttacgg
     5281 attacgggat tccgtgactc gtcgtctgtt gggcggtgtc tgggatggct taacgcaaca
     5341 ggatttgcag atgtatgaag aggcgtattt aagcaacgac gcggaccgcg agtcaccgta
     5401 ttactgtctg tttaataatg atctcactcg cgaagttccg ccctgtttta ttgccgggga
     5461 ggagttcgat ccgctgctgg atgacagccg tctgctttac cagacgttag cggcgcatca
     5521 gcagccctgt gagttcaaac tctacccagg tacgctgcat gcctttttgc attattcacg
     5581 gatgatgaaa accgccgacg aggctcttcg cgacggcgct cagttcttta ccgctcagct
     5641 ttagcgatac gcggcaacaa gattagccat catttcgata tgttccggcg tggcattaag
     5701 cgctggaata tattcatatt ttttcccgcc cgcaccgagg aagacctcac ggttttgctc
     5761 ggcaatctct tccagcgtct ccagacaatc cgcagcaaag cccgggcaca tcacctgtat
     5821 atgacctacg cctttttctc cgagcatttt cagcgtttcg tcggtataag gcatcagcca
     5881 gggttcccgg ccaaagcgcg actgaaaggt catcatcact ttttccggta ccatccccag
     5941 tgcggaagcc agttcgcgag tcgttgtgcg gcaacgttgc gggtaatcat cgccttcatc
     6001 tgcataacgc tggggaatgc catgataaga gagcagcagc agatccggtt cgccatgttt
     6061 ggcaaaagaa gcgcgtacgc tgttcgccag tgcattaatg taatcgtggt tatcggcgta
     6121 atcacgaata aacgatatcc ccggaatgct acgtttgcgc gccagaatgc gtgccagttc
     6181 atcccatacc gcaccgaccg tagaacagga gaattgcgga taaagcggca gcaccacaat
     6241 atgatctaca tgctctgcca ggagttcatc gacggcgctt tccagtgatg gcgagccgta
     6301 gctcatgccc agcgccactg gcgtatccgg taaacgttgt gccaacgcct gctgttgctg
     6361 gcggctgtaa accatcagcg gcgagccgtc ttccatccag acagaggcgt acagcttcgc
     6421 cacacgcggc gagcgcagcg gcaaaatcac gccgcgcagc aacggccacc ataacaaccg
     6481 tgaggtatca accacgcgtc tgtcgcttaa aaattgtttc agatagcgtt ttaccgcttc
     6541 aggtgtgggg gcatcgggcg tacccaggtt tgccagcagg ataccggttt tagtctgacg
     6601 cattaccgcc tcttatcgat tcaacttgtt gataattgta gcggaaaagt gaggaagaag
     6661 aactaattgc tgtgagaggt attgcctgag gtggatgagc agacctgccc gaaagcagat
     6721 ctgctgcggc ttgattagcc gaggattttt tccagatcag cgcgaacttc agcaaccggc
     6781 ttggtaccgt caactttcgc gtatttggtg ttacccgctt ccgcttcttt ggagtagtag
     6841 ccgatcagcg gtgcagtcat ctgatggtat tcaaccagac gtttacgcac ggtttcttcc
     6901 tgatcgtctt tacgggtagt cagttcttca ccggtaacgt cgtctttgcc ttctactttc
     6961 ggcggattga atttaacgtg ataaacacga ccagacggcg catgaacgcg gcggcctacg
     7021 atacgatcaa caatcagttc gtccggtacg tcgaattcca gaacgtaatc aacattgatg
     7081 cccgcttctt tcatcgcgtc tgcctgcgga atggtacgcg ggaagccgtc caacaggaaa
     7141 ccattacggc agtcttcctg agcaatgcgc tctttaacca gcgcgatcac cagttcgtcg
     7201 gtgaccagtt tgccagcatc cataatgtct tttgcttgtt tacccagctc ggagccagat
     7261 ttgaccgcag cacgcagcat atcgccagtg gagatttgcg gaataccata tttctccatg
     7321 atgaactgag cctgagtccc tttccccgcg cccggagcgc caagcagaat gatacgcatt
     7381 gcgaaaatcc ccttaaaatg tgtcgatttt tttgaaaaag cgataaacga taccaccaac
     7441 aggcgattgc ctcaagaaag gcacctgagg ctgaaacggt taatggcgag ataatttgcg
     7501 gaaagtacag atgatgaaaa gaaaaatgcc ggatgacaca aaggtcatcc ggcatgacat
     7561 caggaaacca gcagctggtt catacgacga ataaacaggt tcggatcttc cagcgtgccg
     7621 cgttctgcca gcagcgcctg atccagcagc agttctaccc actcgctgaa cttggcttca
     7681 tcttcagtat ctgccgcacg tttcaccagt acgtgatccg ggttcagttc gaagatgtat
     7741 ttcacttctg gcactttctg gcccgccgca gcgaacagtt tcgccatctg agtgctcatt
     7801 tcgtccgcgt cggtcgaaac gatcgctggc gtatcggtca gacggtgagt cagacggaca
     7861 tctttcacgc gctcgccgag cagagctttc acgcggtcga tgaacggagt cagtgctttc
     7921 tccgcttctt tcgcgctctc atcaacttcg tcagccagtt tttccagcga ctcgtcaact
     7981 ttagacaccg actggaacgg tttaccgtcg aactcagtca gatagttcat catccactca
     8041 tcgatgcggt cggaaagcag cagaacttcg atgcctttct tacgcagcag ttccaggtgc
     8101 gggctgctct tcgctgccgc atagctgtct gcagtgatgt agtagatttt ctcctgccct
     8161 tctttcatgc gggaaacgta gtcttccaga gacacggtct gcgcagaaga atcggtataa
     8221 gtagaagcaa aacgcagcag tttggcgatc gcttcctggt tagcgaaatc ttccgccgga
     8281 ccttctttca gtaccaggcc aaactgttgc cagaaggtct ggtatttttc cgcgtcgtct
     8341 ttcgccagtt tttccagcat ttgcagcaca cgcttggtca gcgcattgcg caggttacgc
     8401 gttaccgtgc tgtcctggag gatttcacgg gaaacgttca gcggcagatc gctggagtca
     8461 atcagaccac gcacgaagcg cagatagttc ggcatgaact gttcagcgtc gtccatgata
     8521 aacacgcgtt gaacgtacag tttcaggccg tgtttatgat cacggttcca catatcccac
     8581 ggagcctggg acgggatgta cagcaggctg gtgtactcct gcttaccttc aacgcggttg
     8641 tggctccagg tcagcggatc gttaaagtcg tgggcgatgt gtttgtagaa ctctttatac
     8701 tcttcatcgg tgatttccga cttgttacga gtccacagcg cctgcgcttt gttgattttt
     8761 gcccaggaga taacggtttc gccatctttc tcttcgcgtt tttcgatctc taccggcagc
     8821 gcgatatggt cggagtattt gctgatgatg gaacgcacgc gccagtcatc gaggaactcg
     8881 tcttcgcctt cacgcagatg cagggtgatt tcagtaccac gatcttcttt ggtgatgtcg
     8941 gcaacggtat attcaccttc gccagccgat tcccagaaga cgccgttttc tggtttttcg
     9001 cctgccgcgc gagtacgcac ggtcactttg tcggccacga taaacgcaga gtagaaacca
     9061 acaccaaact gaccgatcag ctggctgtct ttcgcctggt cagaacccag ggattcgagg
     9121 aatgatttgg taccggattt agcgatagtc cccagatggt caatcacttc gtcgcgggtc
     9181 atccccacgc cgttatcgga gatggtcagc gtgcgcttgt ctttatcgaa agagacacga
     9241 acgcgcagtt cgccatcacc ttcgtacagg tccgggttag atagcgcacg gaaacgcagc
     9301 ttgtccgccg catcggaggc gttagagata agctcacgca ggaagatttc tttattggaa
     9361 tagagagaat ggatcatcag gtgcagaagc tgtttcactt ctgactgaaa accacgagtt
     9421 tcttgtcctt tcatgtaggt ctacctcaat aatgccattt taaggttaaa aacaggatga
     9481 gggagagatg gggacaaggg agaataattt caagcgagag caggtgctcc tgctctcgtt
     9541 tgcttaaaaa cgaatcttat gacgcccggc gagggagtgt gacagcgtgg tgccatcgac
     9601 catttccagt tcgccgccaa ccggtacgcc atgagcgatt cggctggctt ccacgtcata
     9661 ttgcgcgcaa agctcggcaa tgtagttagc ggtagcttca ccttcaaccg tgggattggt
     9721 ggcgaggatc acttcagtga ttttttcctc tgccagacgc tgttccagac gatcaagccc
     9781 gatatcatcc ggaccgatgc cgtccagcgg tgacagatgc cccatcaaca caaaataacg
     9841 accagaaaac tgccccgtct gctcaatggc gtagatgtcc gccggactct ccaccacaca
     9901 gatttgaccg ttttcctgac gacgcggatt cgaacagatg ttacagactt cctgttcggt
     9961 gaaagtgcgg caatcggcgc agtggccgat ttccgacatc gcccgggtga gcgcctgcgc
    10021 cagacgcatc ccgccgctac gatcgcgctg aagcagcgtg aacgccatac gctgcgccga
    10081 cttcgggcca acgcccggca gacagcgcag tgcttccata agctgtgtta acagcgggct
    10141 ggtttgcatc agaacggcat cttaaagcca ggcggcagct gcattccgga ggatacagag
    10201 gccatttttt ctttctgcgt ttcttcaata cgacgtgctg cgtcgttgaa tgctgcagcc
    10261 accaggtctt ccagcatctc tttgtcgtct tccagcaggc tcgggtcgat ctctacgcga
    10321 cggcagttgt gtgcaccgtt gatggtcact tttaccagac ctgcgccaga ttcgccggta
    10381 acttccagct gcgcgatctc ttcctgcatt ttctgcattt tttcttgcat ctgctgggct
    10441 tgcttcatca ggttacccag accgccttta ccaaacatag gtttctctct caatcacgtt
    10501 aaggatgacg aacgtaagct gtgcttacga tcaaatgggg cggatacttt cttcatccag
    10561 ctccgcatcg aaaaaccgac gcagggtctg aatattatta tccgcaataa tggactcgcg
    10621 cgcctgcgca agtttttctt cgtatatcgc ctgacgccac tccagcggcg tacgcaccgc
    10681 gggattatca tcttcaacga tagtcagttc aaccgttgaa ccttttaaca tgctcaacgc
    10741 ttcagccagt ttttgctgtg cgccgcggtt gttcaaatgc cgctgagagg agcgcaaatg
    10801 cagacatact gcgttgtcgc tctcctcttt ccaggcattt aacgccacct gttcgaccag
    10861 ttttggtagc gaaagttggc tcacctgtgc cgcccacggg tcgcgctcaa tggcttctgc
    10921 cgctagcttc gccgccagtt ccggcgtttt ttcatgttcc agcgcttttt tcagcgcctt
    10981 cggcgtggcg accacttctt tttgctgcat caccggagtg gtcgccttcc agcgatacgc
    11041 ttcttttttg gctggcgctt tttccagcgc cgatggcacc ggacgcgcct gaacgcgatc
    11101 ggtgaccgaa gccagtcttt ccagcgcagc gttattcacc ggccgcgcgc gggtagcggc
    11161 tgccggttca ctcttttttg ctttggttgc tccctgcacg cgctgcaact gctggcgcgc
    11221 cgccagcacc tggctggtgg tttccgggag cggtacagtc ggtgcctgct gcggcgctga
    11281 ttgcggcggc accagggttg gcgtcattac tgccgttggc gcgacgggtg caaaggactg
    11341 gcgtggcact tctggctcag gcagcggcat acgcggatgg aacgccagcg cgcgcagcag
    11401 cgtcatctca acgcccatgc ggcggtccgg cgcatacggt aattctttgc gaccaatcaa
    11461 cagcgtctga tagtaaagct gaatatccgt cggcggtatg gtgcgcgcca gttcacgcat
    11521 ccgcagctcg atggcggcca tgtcgttgcc aagtgcagca ggcgaaagtt gtaccatcgc
    11581 aatacgatgc aacaagccga gcatttccac cagcaacgct tcccactcga taccacgggc
    11641 agcggcttca ttaatcagtg ccattacgcg ctcgccgttg ccctcgacca tcgcttcaac
    11701 cagcgacagc gcctgatcgt cgtcaagcgt acccagcatc gcactgaccg cctgggttga
    11761 aacctggccg tcaccgctgg caatcgcctg gtcggtcaga cttaaggcat ctcgcaggct
    11821 gccttcagcg gcgcgcgcca acaattgcag cgcccgcggc tcgtgagcga tatgttcttc
    11881 attgaggatg tgctcaagct gatggcgaat ttgctcgaca tccagcgcct tgagatgaaa
    11941 ttgcagacag cgtgacaaaa tcgtcaccgg caatttctgt ggatcggtcg tcgccagcag
    12001 aaacttaacg tgctccggcg gctcttcaag ggtttttaac agtgcgttaa agctgtggcg
    12061 cgacagcata tgcacttcgt cgatcagata aactttgaaa cgaccacgcg ccggagcgta
    12121 ctggacgtta tccagcaggt cgcgggtatc ttcaactttg gtgcgcgagg cggcgtcgat
    12181 ttcaatcaga tcgacaaagc gcccctgctc gatttcacga cagttatcgc acacgccgca
    12241 cggcgtcgcg gtaatgccgg tttcgcagtt tagccccttc gccagcagtc gggcgataga
    12301 ggtttttccg acgccacggg tgccggaaaa aagataagca tgatgaatac gccctaacga
    12361 caagccgttc gccagtgcgg tcagcacatg ttcctggccg acgacgtcag caaaggtttg
    12421 tgggcgccat tttcgggcta aaacctgata actcattggc aggctctgaa acgctggaag
    12481 gtggattcac gaaggggtaa tgctaacaca gccccgtcag aacggcgagg ctgaatgcac
    12541 atcttgtgct gtccgtagcg tgggcagcac aagactggcg ataattaatg gcccgggaac
    12601 gggacaaggc tgtagctggt aatgccctgt ttttcgagac gctgttcgcc gccgagatcg
    12661 aacaggttga taatgaacgc agcgtcagcc acttcaccac ccagacgacg gatcagttta
    12721 acggtcgctt cgatagtgcc gccggttgcc agcagatcgt ccaccaccag aactttgtcg
    12781 cccggtttga tggcatcaac gtggatttcc agctgatcgg tgccgtattc caggtcgtaa
    12841 ttttcactga tggtttcacg cggcagtttg cccggtttac ggaccggtac aaagccaacg
    12901 cccagaccca gagctaccgg agcgccaaac aagaagccac gcgcttcggt gccgacaact
    12961 ttggtaatgc ccgcattttt gtaacgctca accagcaagt cgatgctgag agcgtaagct
    13021 ttcgggtctt ccagtaagct ggtgacatcg cggaaaagaa tgccgggttt tgggtagtcc
    13081 tgaatgcttt tgatgctatt tttgagatac tcaagctgct gtgcagtcgc ggtcataagt
    13141 gtatgcctgc ttgttacggt ggtactcacg gcgcgtttta aacgtatcaa aagtaaccgt
    13201 tgtttaacct ttgaccgcct gcgcctgtgc tcgaaaacgc cagaatgtac tggctgtacg
    13261 caataattgc aactgcgatt gttgtgcttc agtgcttttc ttgcttttca tcaatcaccg
    13321 gaattcgcca catatagaaa agcaaacagg cgagaattac cagcaacatg atgcgcaccc
    13381 atggcatctg gacgaaccac agagaaatgg caaacgtgag caaaataagc aaaatcgccc
    13441 gcggtttgac gccgcgcggc atcgcatgat gtttctgcca gaaacgtaga tagctgccaa
    13501 accatgagcg gtacagcaac caggcgtgaa agcgcgggga agaacgggca aagcaccagg
    13561 ccgccagcag gataaacggc gtcgtcggta ataccggtaa taccacgccc agcgtaccca
    13621 gcactaccgc cagccagcca atgatgatta aaatgattcg ttgcatattg agtgttgtca
    13681 gcttacagag tggctacttt agcataacaa ttatcatttt cattgaggtc ttatcgtgaa
    13741 aaccgccctg ctgctggaaa aactggaagg acagctcgct acgctgcgtc agcgttgtgc
    13801 cccggtgtca cagttcgcca cgctaagtgc tcgtttcgac aggcatcttt ttcagactcg
    13861 tgcgacaaca ctacaggctt gtctcgacga ggcgggcgat aatctggctg cgcttcgtca
    13921 tgcagttgag cagcaacagc tgccgcaagt ggcctggctg gcggaacatc tggcggcaca
    13981 actggaagcc atcgcgcgtg aagccgccgc ctggtcattg cgcgagtggg acagtgcgcc
    14041 accgaaaatt gcccgctggc agcgtaaacg tattcagcat caggattttg agcggcggct
    14101 acgtgagatg gttgccgaac gcagagcccg cctggcgcgg gtgaccgatc tcgtggaaca
    14161 gcaaacgctg catcgtgaag tggaagccta tgaagcgcgc ctggcacgct gccgccatgc
    14221 gctggaaaaa atcgaaaaca ggctagcgcg cttaacccgc tagcaatgga aagagtatgt
    14281 cacttgaaaa tgcccctgac gatgtcaaac tggctgtcga tttgattgtg ctgctggagg
    14341 aaaatcagat ccctgccagt accgtgctgc gcgcgctgga tattgtaaag cgtgattatg
    14401 aaaagaaatt aacgcacgat gatgaggcgg aaaagtgaga aataaatggg gccgctaaat
    14461 gtcccctaag attgcgggca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    14521 accgctcctg caatatattg aattcttgcg gtttcgtagg ccggataagg cggtcacgcc
    14581 gcatccggca tgaacaacgc gcactttgtc agtaatctcg gggcaactaa atgtcgcccc
    14641 ctttggttat tacccaaccg ctggtgtcgg atcgtcgcct ttgtagtcgc gttttacttc
    14701 cgtcacctca tcgcccttct cgttgtgcag atgcacttca agctggttaa aggcaatgtt
    14761 gatgtcgttt tcacggcaca gctgatcgat agtacggttc agctcatcga cagtacggct
    14821 acggtcacgc agttcacgca catacagacg cagctcatga tccaacgtgc tggcaccaaa
    14881 tgccgtaaag aagacttccg gcattggttc gtgcatcacc cttgggtgct cagtcgccgc
    14941 cttcagtaac actttacgca ctttttccag atcggagcca taggccacgc cgagacggat
    15001 caccagacgc gtagtagtgt cagtcaacga ccagttgatc agacgctcgg taacaaacgc
    15061 tttgttcggg atgatcactt ctttgcgatc gaaatcggta atcgttgtcg cacgaatacg
    15121 gatcttactt accgtccccg agaagctacc aatggttacc gtatcgccaa tacgcaccgg
    15181 acgttcgaac agaatgatca aaccggagac gaagttaccg aaaatttctt gtaaaccaaa
    15241 accaagacct acggataatg ctgcggccag ccactggagt ttatcccaag agacgcccag
    15301 cgatccgaac accgtcatcg caccaacggc aataatgatg tagttaagga tggtagtaat
    15361 ggcatacgac gcgccctggc gcatattcag tcgcgagagc accagcactt ccagtaaacc
    15421 aggcaggttg cgaatcaacg cccaggccac cattgaggcg ataatcgcaa acaacagact
    15481 gcccatggtg acgtttttca ccaccgcagc gccagcttca gtgccgttgt aatgccagag
    15541 cgtgatgctg tcgagatagc tgaacacggt gatcaaatcg gaccaaattg cccagaacat
    15601 gacaccgaac agcgcaaaca tcagcaacat ggtaatacgc agcgtctgct ggttaacttg
    15661 ctccagtgca atggtgggtt cttccggcgg ttcggcacct tctgcgccct ctttcaccag
    15721 attctgccga cgcgccagcg cacgacgcca ggcgatacgc cgcgccgcta cgcttaagcc
    15781 gcgcaatacc gtctggtaga gcaagttcca gatgatcacc agataaacgg tttcaatcca
    15841 acgcccggag aggcgcagcg tggtgtagaa ataacccgtg gcagtcagca ccatcaatgc
    15901 aatcgggata atcgacagca cggtaatagt gactagtcgc atggtgtgcg actctttatc
    15961 acgccagctt tcgcggcaca ttggccatac caggaaggca atcagcagca ggttgaagaa
    16021 aatcatcgcc tgccccagca catcatccat cagatgcagc ggggaaagtt ctgccaccac
    16081 agaccagaaa tggataggca gcaacgcgag actgatgcgg acaatttgcc gacgccagtg
    16141 gctggtctgc tgctccggca tgccgaagtg acgcacggca acgccatttt tctccagtac
    16201 cttccagcac aggccaaaca ccagccagaa aatcgccagt tttttactga acgaccatag
    16261 cagttcgctg atattgagct gcatggtcaa cagaatcagg ccaaccgcga gaataatcag
    16321 gcacaccggc agcgcgcgga tcaggtcgat aaggatcgct tttggtgtgt tgagctggct
    16381 atcgttacgc agggaaccca cagcggaagc cagtttctgt tgatacgctt tcagccagcc
    16441 cagacgccag tggatcagcc cggcaatcaa cagcagcggc aaaccggcga ggaaagcgat
    16501 aaaaacggca ggccaggctt tttcccagtt caccgtgatt ttcatcgact taaattcatc
    16561 tttcaggctt tgcgggaacg ctttgatcca gtcccagtcc attggacggt tactgttcac
    16621 ccaaaagatt tgctgagtca ggatggattt caggtttttc gatacgctca ttaactgctg
    16681 ctggttgatt tgcaggttaa tggccatcat cagctggtta cccaactgtt tgttgagttg
    16741 atccagcaat tcgcgacgca tatcaaccac ttgcaataac gcatcgtgaa cttcgctgtt
    16801 gacttcgttg gtgtgacctt cttccagttt gttgacgaac gcatcgctct ggaagagtgc
    16861 atcacgctgc tggttaactt caaactgttc gagacgcaaa tccgcgatgc ggttggtcat
    16921 gttttccagt tcatccgccg agggcagcgt ttgttgttgc tggtaaagga tacgagacaa
    16981 cagcaggctg cccttcagga cggcaatctg ctctttaata ttgcgttccg attgcagcgc
    17041 ccgctccagc cagtttttga ctttaatgtt ttgctgcatc aactgattac cgttttcagt
    17101 cgcggtaatc agacgctgac ttaactgctg gttaatttcc agttcctgtt tcaccagcgg
    17161 attagcctga atacgcgcgg cttcatccgg ggagacggct tcctgcgccg ttttttcagt
    17221 taaagtcagg cgcttgctgt ttaccgcttc ttgcaacagc tgcaactgat gctccagacg
    17281 ggcgctgttc gccgtcacgt aatcacgttg cttttgcaag gtatcctgca agacggtgtt
    17341 cccttccagg cttttacgct gctggtcaat ctccgcattc agcaacgcct gctgagcctg
    17401 cattaacact ttctggctgg gacgtaaggc agtctcgccg acatcagtcc catccagacg
    17461 actgcgaatt tgttgcagct gctgcgaagc gttatacatc gcattttgca cgcgttcggg
    17521 ctgcgtctgt aacgaaacca gctggctgtt ataagacgcc agatcgtttt gtgcgttttg
    17581 caaatcgtcc agcgcctggg caacgcgagt ttccagctgg cgcaacgaaa gcgtactcag
    17641 aattttgcgc gtttcttcgt cgttatcgac atcgctaagt gctgttaacg ccgcggtcgc
    17701 ctggcgcatt ttttccggcg cttcagcgac tttttgccgt agctgaactg tctcttcttt
    17761 tatgcgatcg attttatcga gggtggctaa tgtatctgtc agatcctgct gcaccagttt
    17821 gtcctgagca gaaagatctt tttgtttatt tagtgagtca agttgcgcct gcaggtccgc
    17881 ttttgtcggc agatcaccat tcgatgacgc ccgcgcaaac gccgtgttct ggcataacaa
    17941 gacaaaaaca aaagcaataa atgctgaaaa aacaaaatgc cgtgatcgtt tgtaatactg
    18001 gaacatagtc atgatgaatg aaggtttctg aacctgaaga acgacctgaa aaagtcaaac
    18061 cgcaagaata tcacgacgca gtgaaccaga atagcaacga cgaaaatgtc caggaaaaat
    18121 cctggagtca gattcagggt tattcgttag tagcaggatt acgaagcgtg gggcacagga
    18181 gatacatctc cagtaagatg gcgacgtaat cgcgggcttc ttttttaaga tcaaaagatt
    18241 gcggggcaaa gagccagttt tccatcaggc cggaaatata gccgcgcata ataattgctg
    18301 cccgacgcgt cattaaatcc gcaggcaaca ttttcgcttc aatacaatgt tttaacgttt
    18361 gttctatacg gtcataactt tccagacaga gattacgttg tgcctgttgc acaacagcca
    18421 tttctccgac aaattcgcat ttgtggaata taatctccat caataatcga cgtcgttctt
    18481 ctgtcaccgt ggattcaaga acatgaatta atatctctct taatactgag agtggatcgc
    18541 cagggaattt tgcctgatac tcaagctcta gttcaccaat attggattct gacagttccc
    18601 agatctcact gaacaaatcc gacttgtctt taaaatgcca gtagattgca ccgcgcgtaa
    18661 cgccagctgc ttttgcaatc tcgcccagcg aggtggatga taccccctgc tgtgagaaaa
    18721 gacgtagagc cacatcgagg atgtgttggc gcgtttcttg cgcttcttgt ttggtttttc
    18781 gtgccatatg ttcgtgaatt tacaggcgtt agatttacat acatttgtga atgtatgtac
    18841 catagcacga cgataatata aacgcagcaa tgggtttatt aacttttgac cattgaccaa
    18901 tttgaaatcg gacactcgag gtttacatat gaacaaaaac agagggttta cgcctctggc
    18961 ggtcgttctg atgctctcag gcagcttagc cctaacagga tgtgacgaca aacaggccca
    19021 acaaggtggc cagcagatgc ccgccgttgg cgtagtaaca gtcaaaactg aacctctgca
    19081 gatcacaacc gagcttccgg gtcgcaccag tgcctaccgg atcgcagaag ttcgtcctca
    19141 agttagcggg attatcctga agcgtaattt caaagaaggt agcgacatcg aagctggtgt
    19201 ctctctctat cagattgatc ctgcgaccta tcaggcgaca tacgacagtg cgaaaggtga
    19261 tctggcgaaa gcccaggctg cagccaatat cgcgcaattg acggtgaatc gttatcagaa
    19321 actgctcggt actcagtaca tcagtaagca agggtacgat caggctctgg ctgatgcgca
    19381 acaggcgaat gctgcggtaa ctgcggcgaa agctgccgtt gaaactgcgc ggatcaatct
    19441 ggcttacacc aaagtcacct ctccgattag cggtcgcatt ggtaagtcga acgtgacgga
    19501 aggcgcattg gtacagaacg gtcaggcgac tgcgctggca accgtgcagc aacttgatcc
    19561 gatctacgtt gatgtgaccc agtccagcaa cgacttcctg cgcctgaaac aggaactggc
    19621 gaatggcacg ctgaaacaag agaacggcaa agccaaagtg tcgctgatca ccagtgacgg
    19681 cattaagttc ccgcaggacg gtacgctgga attctctgac gttaccgttg atcagaccac
    19741 tgggtctatc accctacgcg ctatcttccc gaacccggat cacactctgc tgccgggtat
    19801 gttcgtgcgc gcacgtctgg aagaagggct taatccaaac gctattttag tcccgcaaca
    19861 gggcgtaacc cgtacgccgc gtggtgatgc caccgtactg gtagttggcg cggatgacaa
    19921 agtggaaacc cgtccgatcg ttgcaagcca ggctattggc gataagtggc tggtgacaga
    19981 aggtctgaaa gcaggcgatc gcgtagtaat aagtgggctg cagaaagtgc gtcctggtgt
    20041 ccaggtaaaa gcacaagaag ttaccgctga taataaccag caagccgcaa gcggtgctca
    20101 gcctgaacag tccaagtctt aacttaaaca ggagccgtta agacatgcct aatttcttta
    20161 tcgatcgccc gatttttgcg tgggtgatcg ccattatcat catgttggca ggggggctgg
    20221 cgatcctcaa actgccggtg gcacaatatc ctacgattgc accgccggca gtaacgatct
    20281 ccgcctccta ccccggcgct gatgcgaaaa cagtgcagga cacggtgaca caggttatcg
    20341 aacagaatat gaacggtatc gataacctga tgtacatgtc ctctaacagt gactccacgg
    20401 gtaccgtgca gatcaccctg acctttgagt ctggtactga tgcggatatc gcgcaggttc
    20461 aggtgcagaa caaactgcag ctggcgatgc cgttgctgcc gcaagaagtt cagcagcaag
    20521 gggtgaacgt tgagaaatca tccagcagct tcctgatggt tgtcggcgtt atcaacaccg
    20581 atggcaccat gacgcaggag gatatctctg actacgtagc ggcgaatatg aaagatgcca
    20641 tcagccgtac gtcgggcgtg ggtgatgttc agttgttcgg ttcacagtac gcgatgcgta
    20701 tctggatgaa cccgaatgag ctgaacaaat tccagctaac gccggttgat gtcattaccg
    20761 ccatcaaagc gcagaacgcc caggttgcgg cgggtcagct cggtggtacg ccgccggtga
    20821 aaggccaaca gcttaacgcc tctattattg ctcagacgcg tctgacctct actgaagagt
    20881 tcggcaaaat cctgctgaaa gtgaatcagg atggttcccg cgtactgctg cgtgatgtgg
    20941 cgaagattga gctgggtggt gagaactacg acatcatcgc agagtttaac ggccaaccgg
    21001 cttccggtct ggggatcaag ctggcgaccg gtgcaaacgc gctggatacc gctgcggcaa
    21061 tccgtgctga actggcgaag atggaaccgt tcttcccgtc gggtctgaaa attgtttacc
    21121 cgtacgacac cacgccgttc gtgaaaatct ctattcacga agtggttaaa acgctggtcg
    21181 aagcgatcat cctcgtgttc ctggttatgt atctgttcct gcagaacttc cgcgcgacgt
    21241 tgattccgac cattgccgta ccggtggtat tgctcgggac ctttgccgtc cttgccgcct
    21301 ttggcttctc gataaacacg ctaacaatgt tcgggatggt gctcgccatc ggcttgttgg
    21361 tggatgacgc tatcgttgtg gtagaaaacg tggagcgtgt tatggcggaa gaaggtttgc
    21421 cgccaaaaga agctacccgt aagtcgatgg ggcagattca gggcgctctg gtcggtatcg
    21481 cgatggtact gtcggcggta ttcgtaccga tggccttctt tggcggttct actggtgcta
    21541 tctatcgtca gttctctatt accattgttt cagcaatggc gctgtcggta ctggtagcgt
    21601 tgatcctgac tccggctctt tgtgccacca tgctgaaacc gattgccaaa ggcgatcacg
    21661 gggaaggtaa aaaaggcttc ttcggctggt ttaaccgcat gttcgagaag agcacgcacc
    21721 actacaccga cagcgtaggc ggtattctgc gcagtacggg gcgttaccta gtgctgtatc
    21781 tgatcatcgt ggtcggcatg gcctatctgt tcgtgcgtct gccaagctcc ttcttgccag
    21841 atgaggacca gggcgtattt atgaccatgg ttcagctgcc agcaggtgca acgcaggaac
    21901 gtacgcagaa agtgctcaat gaggtaacgc attactatct gaccaaagaa aaaaacaacg
    21961 ttgagtcggt gttcgccgtt aacggcttcg gctttgcggg acgtggtcag aataccggta
    22021 ttgcgttcgt ttccttgaag gactgggccg atcgtccggg cgaagaaaac aaagttgaag
    22081 cgattaccat gcgtgcaaca cgcgctttct cgcaaatcaa agatgcgatg gttttcgcct
    22141 ttaacctgcc cgcaatcgtg gaactgggta ctgcaaccgg ctttgacttt gagctgattg
    22201 accaggctgg ccttggtcac gaaaaactga ctcaggcgcg taaccagctg cttgcagaag
    22261 cagcgaagca ccctgatatg ctgaccagcg tacgtccaaa cggtctggaa gataccccgc
    22321 agtttaagat tgatatcgac caggaaaaag cgcaggcgct gggtgtttct atcaacgaca
    22381 ttaacaccac tctgggcgct gcatggggcg gtagctatgt gaacgacttt atcgaccgcg
    22441 gtcgtgtgaa gaaagtttac gtcatgtcag aagcgaaata ccgtatgctg ccggatgata
    22501 tcggcgactg gtatgttcgt gctgctgatg gtcagatggt gccattctcg gcgttctcct
    22561 cttctcgttg ggagtacggt tcgccgcgtc tggaacgtta caacggcctg ccatccatgg
    22621 aaatcttagg ccaggcggca ccgggtaaaa gtaccggtga agcaatggag ctgatggaac
    22681 aactggcgag caaactgcct accggtgttg gctatgactg gacggggatg tcctatcagg
    22741 aacgtctctc cggcaaccag gcaccttcac tgtacgcggt ttcgttgatt gtcgtattcc
    22801 tgtgtctggc ggcgctgtac gagagctggt cgactccgtt ctccgttatg ctggtcgttc
    22861 cgctgggggt tatcggtgcg ttgctggctg ccaccttccg tggcctgacc aatgacgttt
    22921 acttccaagt aggactgctc acaaccattg ggttgtcggc gaagaacgcg atacttatcg
    22981 tcgaattcgc caaagacttg atggataaag aaggtaaagg tctgattgaa gcgacgcttg
    23041 atgcggtgcg gatgcgttta cgtccgatcc tgatgacctc gctggcgttt atcctcggcg
    23101 ttatgccgct ggttatcagt actggtgctg gttccggcgc gcagaacgca gtaggtaccg
    23161 gtgtaatggg cgggatggtg accgcaacgg tactggcaat cttcttcgtt ccggtattct
    23221 ttgtggtggt tcgccgccgc tttagccgca agaatgaaga tatcgagcac agccatactg
    23281 tcgatcatca ttgatacaac atgtaatcac taaggccgcg taagcggcct tttttatgca
    23341 taacctacga acattaagga gtaattgaac caccaactca ggatctcata cgaaaaccag
    23401 tattaaccac gtataaaatt cataaaaaat actgattgtt agttaattta tattaagtag
    23461 cgctaataga tttaataatc cataatcatt tagaggctat tcttaattat ttgcggtaat
    23521 tctttattca ttcctcggtt attacgtcat attcagagca atcctggtat tagtgtcacc
    23581 aatttcatct ggcgataatc ctgaaatgtt atgaacagtt cgagcaaact gcttttacct
    23641 gctgcgggtt agtgctagta tgaaaaagtg agtcctgtcc cgattccttc ctaattgtaa
    23701 tgtttcgtaa taatgcgatg aaaacctgca aagagtggct tatagttaag ctaacaaacg
    23761 agagggcaag tctaggtcag taagtttttt ccatcccgaa aggtgtccgt tagttcaacc
    23821 actaagaagg ggacgcgtta tggatgaata ctcacccaaa agacatgata tcgcacagct
    23881 taagtttctc tgtgaaaccc tgtatcatga ctgtcttgca aaccttgaag aaagcaatca
    23941 tggctgggtt aacgacccaa cctcggcgat caacctccag ttgaatgaac tgattgagca
    24001 tattgcgacc ttcgcactta attacaaaat taagtataat gaagacaata agctcattga
    24061 gcagatcgac gaatatctgg atgacacctt tatgttgttc agcagttatg gtattaatat
    24121 gcaggctctt cagaaatggc ggaagtcagg taatcgacta ttccgttgtt ttgtcaatgc
    24181 gacgaaagag aatcctgcga gtttatcttg ttagaattat tacaaccatg ggtagaagta
    24241 tgtccgaaaa acctttaaca aaaaccgatt atttaatgcg tttacgtcgt tgccagacaa
    24301 ttgacacgct ggagcgtgtt atcgagaaaa ataaatacga attatcagat aatgaactgg
    24361 cggtatttta ctcagccgca gatcaccgcc tcgccgaatt gaccatgaat aaactgtacg
    24421 acaagatccc ttcctcagta tggaaattta ttcgctaata aataattcgc tttcggagct
    24481 ataaccggct gtttattaag aattttatac tttttcgcca tgaagacata ccctatgtga
    24541 tctttatcac acagatgtaa tgagaacgtt ctcttcactg acttttcgtc ttactgtgtt
    24601 gccgcatttt cagcaaccgg aggtcagtaa tgagcacaga aaaagaaaag atgattgctg
    24661 gtgagttgta tcgctcggca gatgagacgt tatctcgcga tcgcctgcgc gctcgtcagc
    24721 ttattcaccg atacaatcat tccctagcgg aagagcacac attacgccag caaattctcg
    24781 ctgatttatt cggtcaggtg acagaggctt atattgagcc aacgtttcgc tgtgattatg
    24841 gctataacat ttttctcggc aataattttt tcgccaactt cgattgcgtg atgcttgatg
    24901 tctgccctat tcgcatcggt gataactgta tgttggcacc aggcgttcat atctacacgg
    24961 caacacatcc catcgaccct gtagcacgta atagcggtgc tgaactgggg aaacccgtca
    25021 ccatcggtaa taacgtctgg attggcggac gcgcggtcat taaccctggt gtgaccattg
    25081 gtgataacgt cgtggtagct tcaggtgcag ttgtcacaaa aggtgtcccg gacaacgttg
    25141 tcgtgggcgg taatccagcc agaataatta aaaaattgta atcggttttt cgcaactgtt
    25201 atgcaaaatt gtggtagatc tgttacttcc cctctactat tcccacgtta aaatagggtg
    25261 ttccctggaa agttgcagat accacgaagg caaacgatga ccgaaataca acgcctgctg
    25321 accgaaacga ttgagtctct gaatacccgc gaaaaacgcg acaacaaacc ccgctttagt
    25381 atcagtttta tccgtaaaca tccggggctg tttatcggta tgtacgttgc tttttttgcc
    25441 accctggcgg tgatgttgca gtccgaaacg ctgtcaggct ctgtctggct actggttgta
    25501 ttatttatcc tgcttaatgg tttcttcttt ttcgatgtct acccacgcta ccgctatgaa
    25561 gatatcgacg tgctggattt ccgcgtttgc tataacggcg aatggtacaa cacgcgcttt
    25621 gtacctgccg cgctggttga agccatcttg aactctccgc gtgtcgcgga tgttcataag
    25681 gaacaactgc aaaaaatgat cgtccgtaaa ggtgaactgt ctttttacga tatttttacc
    25741 ctcgctcgcg ccgaatcaac atcttaagtt agggttacat accaggcgta aagctctgcg
    25801 cctggtcaaa tgacaatgat cgtttccacc catcacttca tgaaatacca gctctacctc
    25861 cttatctcct gccagccttt ttccacaatc agatatactt tccctacact gtgttaataa
    25921 ggatatgctg gtgagaacac gacatctggt cggccttatt tcgggagtac tgattctttc
    25981 agtattgctg cctgtcggct taagcatctg gctggcccat cagcaggtag aaacatcgtt
    26041 tattgaagag ctggatacct attcctcccg cgtcgctatt cgagccaata aggtggcgac
    26101 acaagggaaa gatgcgctgc aggagctgga aagatggcaa ggcgctgcct gtagcgaagc
    26161 ccatctcatg gaaatgcgtc gggtatctta cagttatcgc tatattcagg aagtggttta
    26221 tatcgataac aacgttcccc agtgttcgtc tctggagcat gaaagtccgc ccgatacctt
    26281 tcccgagcca ggtaaaattt cgaaagatgg ttatcgtgtc tggttaacat cgcataacga
    26341 tttaggcatt atccgttaca tggtcgccat gggaacggca cattatgtcg tcatgatcga
    26401 ccccgcttcc tttattgatg tcattcccta tagctcatgg caaattgatg ccgccattat
    26461 tggcaatgcc cataacgttg tcataaccag cagcgatgaa attgctcagg gaattattac
    26521 caggctacaa aaaacacccg gtgagcatat cgaaaataat gggatcattt acgatatcct
    26581 gcccttcccg gagatgaata tttcgatcat cacctgggct tcaacgaaaa tgttgcagaa
    26641 aggctggcat cggcaagtct ttatttggtt accgctcggg ttggtgattg gcctgctggc
    26701 agcgatgttt gtgctgcgta ttttgcgccg tatccagtca ccgcatcatc ggctgcagga
    26761 tgctatcgaa aatcgtgata tttgcgtgca ctatcagccg attgtctcct tagccaatgg
    26821 caaaattgtc ggtgctgagg cactggcgcg ctggccgcag acagacggta gttggttgtc
    26881 accagatagt tttattccgc tggcacagca aacgggcctt tctgagccat tgacgctact
    26941 gattataaga agcgtctttg aagatatggg cgactgcttg cgtcagcatc cacagcagca
    27001 tatttcgatc aatcttgaat ccaccgtgct cacctcggaa aaaatcccgc aattgctgcg
    27061 tgaaatgatc aatcactatc aggttaatcc cagacagatc gcgcttgaac tcactgaacg
    27121 cgagtttgcc gatccgaaaa ccagcgcccc gataatttct cgctaccggg aggcgggcca
    27181 tgaaatttat cttgatgatt ttggtacggg gtattcaagt ttaagttatt tacaggatct
    27241 ggatgtcgac attctgaaga tcgataaatc tttcgttgat gcgctggaat ataaaaatgt
    27301 cacgccgcat atcatcgaaa tggcaaaaac actgaaactg aaaatggtag cggagggaat
    27361 cgaaaccagt aaacaagaag agtggctacg ccagcatggc gtgcactacg gtcagggctg
    27421 gctctacagc aaggcattac cgaaagaaga tttcttacgc tgggccgagc aacatttgtg
    27481 aatttttggg ctgacggcac gccgcacagc ccgtcacgct acctattcgt cgatgattga
    27541 ctcgaatccg ccatagatca ttcgcttgcc gtcaaacggc atggactcgc cgaactcttt
    27601 catccgtggg tccgacatca tcttttgatt agctgcgtcg cggacctctt ttgaagggta
    27661 ttcaatccag ctaaagacaa cctcttcatt ctcttccgct ttcaccgcca tacgaaaatc
    27721 ggtcactttg ccatccggta catcgctggc ccagcattcg acaatacgaa gcgcgccaaa
    27781 ctctttaaac aatggcgcgg ccttagcggc catttcccga taggcatcct ttttatcggc
    27841 aggaacggca accacaaaac catcaacata cttcatcaga catacctccg aagcgtactg
    27901 cgcagccaga caattcttgc gacgtcagtt gtggttaaag tttagccatg ctgggatttt
    27961 ttgacgaaac ggtgggaggc agaaatgggt gggggccctg ccagctacat cccggcacac
    28021 gcgtcatctg ccttggctgc ttccttccgg acctgacctg gtaaacagag tagcgttgcg
    28081 ggagaaccaa cagagccccc attgagagcg ttgaaaacca acgcgcaggc gcattatcac
    28141 tgctgccccg gctaattgca acccattgcg tgccagatgc tggtttcttg cgcaatgcga
    28201 cttcactcgg gcgtttttcc agattatgct gaaaaattac ctcaggaaga aaagatggaa
    28261 aaagaagatt catttcccca acgcgtctgg caaatcgtcg ccgctattcc cgaaggctat
    28321 gtcaccactt acggtgatgt ggcgaaactg gcgggatcgc cccgcgccgc gcgccaggtg
    28381 ggcggtgtgt taaagcgtct ccctgaaggc agcaccttac cctggcaccg ggtggttaat
    28441 cgccatggca caatttcgct aaccgggccg gatttacagc gtcagcgaca ggcattactg
    28501 gcagaaggtg tgatggtatc gggaagcggg caaatcgact tgcagcgtta tcgctggaac
    28561 tactaaccct ctccgg
//
LOCUS       DADLTI010000037        28370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLTI010000037 DADLTI010000000
VERSION     DADLTI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.037"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JN755_001471"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JN755_001471"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7380314.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JN755_001472"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JN755_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7380315.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JN755_001473"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JN755_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7380316.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JN755_001474"
     CDS             2778..4127
                     /locus_tag="JN755_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7380317.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JN755_001475"
     CDS             4278..5927
                     /locus_tag="JN755_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7380318.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JN755_001476"
     CDS             complement(6081..7373)
                     /locus_tag="JN755_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7380319.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JN755_001477"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JN755_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7380320.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JN755_001478"
     CDS             complement(9197..9511)
                     /locus_tag="JN755_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7380321.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JN755_001479"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JN755_001479"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7380322.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JN755_001480"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JN755_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7380323.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JN755_001481"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JN755_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7380324.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JN755_001482"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JN755_001482"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7380325.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JN755_001483"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JN755_001483"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7380326.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JN755_001484"
     CDS             15750..17468
                     /locus_tag="JN755_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7380327.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JN755_001485"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JN755_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7380328.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JN755_001486"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JN755_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7380329.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JN755_001487"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JN755_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7380330.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JN755_001488"
     CDS             complement(20170..20859)
                     /locus_tag="JN755_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7380331.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JN755_001489"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JN755_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JN755_001490"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JN755_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7380332.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVTALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JN755_001491"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JN755_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27750)
                     /locus_tag="JN755_001492"
     CDS             complement(26812..27750)
                     /locus_tag="JN755_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7380333.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27769..28044)
                     /locus_tag="JN755_001493"
     CDS             complement(27769..28044)
                     /locus_tag="JN755_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380334.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28253..>28370)
                     /locus_tag="JN755_001494"
     CDS             complement(28253..>28370)
                     /locus_tag="JN755_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380335.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6472 a         7177 c         7615 g         7106 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcag ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tcattttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgt cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc
    27301 tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt
    27361 cgcggcggca cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc
    27421 gtcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggc
    27481 tttcgccagg ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt
    27541 caccgcctgg ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac
    27601 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
    27661 gataacgaaa acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact
    27721 gcgaggtgct tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc
    27781 cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc
    27841 cagattgata tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc
    27901 aatggcgcag aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa
    27961 tgagcatccc gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc
    28021 catgcgagag agaactgttg gcatattaaa tacatcctgt ctgctgcgaa caatatctgt
    28081 agtgaatatc aatatccatt gaaggcgtca gtatggctat tttctcgatg aataaaaata
    28141 gctttatcca gccaccgcat tcataactta tatattttat ttattaattg gctgtatata
    28201 tttttaaaat aaatgctttt gggggcttca gcgcccccaa aagcgagtga cattaatttg
    28261 gggttacgat attcacccac aaatcgaaat tatccagaca ggcgataatt tcttccagtt
    28321 tcgcgccatt gccaataatt ttggctctct tcgctttcac cagatccgcc
//
LOCUS       DADLTI010000038        28027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLTI010000038 DADLTI010000000
VERSION     DADLTI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.038"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JN755_001495"
     CDS             complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JN755_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7380336.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
     regulatory      510..658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            762..1415
                     /gene="ribB"
                     /locus_tag="JN755_001496"
     CDS             762..1415
                     /gene="ribB"
                     /locus_tag="JN755_001496"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7380337.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1676..1846
                     /locus_tag="JN755_001497"
     CDS             1676..1846
                     /locus_tag="JN755_001497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380338.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JN755_001498"
     CDS             complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JN755_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7380339.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2793..3608
                     /gene="ygiD"
                     /locus_tag="JN755_001499"
     CDS             2793..3608
                     /gene="ygiD"
                     /locus_tag="JN755_001499"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7380340.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3646..4806)
                     /locus_tag="JN755_001500"
     CDS             complement(3646..4806)
                     /locus_tag="JN755_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7380341.1"
                     /translation="MERVSITDRPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4812..5483)
                     /locus_tag="JN755_001501"
     CDS             complement(4812..5483)
                     /locus_tag="JN755_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7380342.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JN755_001502"
     CDS             complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JN755_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7380343.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7317..7946
                     /gene="nudF"
                     /locus_tag="JN755_001503"
     CDS             7317..7946
                     /gene="nudF"
                     /locus_tag="JN755_001503"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7380344.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7947..8369
                     /locus_tag="JN755_001504"
                     /pseudo
     CDS             7947..8369
                     /locus_tag="JN755_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8394..9221
                     /gene="cpdA"
                     /locus_tag="JN755_001505"
     CDS             8394..9221
                     /gene="cpdA"
                     /locus_tag="JN755_001505"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7380345.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9221..9802
                     /gene="yqiA"
                     /locus_tag="JN755_001506"
     CDS             9221..9802
                     /gene="yqiA"
                     /locus_tag="JN755_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7380346.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9831..11723
                     /gene="parE"
                     /locus_tag="JN755_001507"
     CDS             9831..11723
                     /gene="parE"
                     /locus_tag="JN755_001507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7380347.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11771..12085)
                     /locus_tag="JN755_001508"
     CDS             complement(11771..12085)
                     /locus_tag="JN755_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7380348.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JN755_001509"
     CDS             complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JN755_001509"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7380349.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13016..13348
                     /locus_tag="JN755_001510"
     CDS             13016..13348
                     /locus_tag="JN755_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7380350.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JN755_001511"
     CDS             complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JN755_001511"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7380351.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JN755_001512"
     CDS             complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JN755_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7380352.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15551..15943
                     /gene="ygiW"
                     /locus_tag="JN755_001513"
     CDS             15551..15943
                     /gene="ygiW"
                     /locus_tag="JN755_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7380353.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15996..16478
                     /locus_tag="JN755_001514"
     CDS             15996..16478
                     /locus_tag="JN755_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7380354.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17024..19282
                     /gene="parC"
                     /locus_tag="JN755_001515"
     CDS             17024..19282
                     /gene="parC"
                     /locus_tag="JN755_001515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7380355.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19516..20253
                     /gene="plsC"
                     /locus_tag="JN755_001516"
                     /gene_synonym="parF"
     CDS             19516..20253
                     /gene="plsC"
                     /locus_tag="JN755_001516"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7380356.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20328..21740
                     /gene="ftsP"
                     /locus_tag="JN755_001517"
     CDS             20328..21740
                     /gene="ftsP"
                     /locus_tag="JN755_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7380357.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21851..24070
                     /locus_tag="JN755_001518"
     CDS             21851..24070
                     /locus_tag="JN755_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7380358.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24113..24370)
                     /gene="yqhH"
                     /locus_tag="JN755_001519"
     CDS             complement(24113..24370)
                     /gene="yqhH"
                     /locus_tag="JN755_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7380359.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24421..25347)
                     /locus_tag="JN755_001520"
     CDS             complement(24421..25347)
                     /locus_tag="JN755_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7380360.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25547..26374)
                     /gene="dkgA"
                     /locus_tag="JN755_001521"
     CDS             complement(25547..26374)
                     /gene="dkgA"
                     /locus_tag="JN755_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7380361.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26479..27642)
                     /gene="yqhD"
                     /locus_tag="JN755_001522"
     CDS             complement(26479..27642)
                     /gene="yqhD"
                     /locus_tag="JN755_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7380362.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27836..>28027
                     /locus_tag="JN755_001523"
     CDS             27836..>28027
                     /locus_tag="JN755_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7380363.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6568 a         6945 c         7445 g         7069 t
ORIGIN      
        1 gtgatgatac tctgaatggg attaagcgat aatgtaatag aaaacactgt aaattagagg
       61 attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct
      121 ctggatctgg ctgctttttg atctcagtac tgcgattttc cagttcgctg attctttgtt
      181 caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt
      241 cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct
      301 ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga
      361 tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta
      421 ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg
      481 ccgctaatca ttagagttat agtgaatccg cttattctca gggcggggcg aaattcccca
      541 ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac
      601 agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat
      661 tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc
      721 cctgattctg gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt
      781 cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg cgtgaaggac
      841 gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg
      901 cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt
      961 gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata
     1021 acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta
     1081 ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa
     1141 aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg
     1201 tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta
     1261 aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag
     1321 agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg
     1381 cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta
     1441 atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt
     1501 taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat
     1561 gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga
     1621 tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt
     1681 tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt
     1741 gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa
     1801 atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc
     1861 tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg
     1921 tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg
     1981 ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca
     2041 ccataattcc tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac
     2101 cgaggattaa ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc
     2161 acagaatcgc ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac
     2221 cttcagggat attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc
     2281 tgctcgccgt gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag
     2341 tgagcagaat ggctgtgcgc ttgatcgatt ttggcaacag ctgcaccgat ttttgcatta
     2401 aatcctgcgg atgcgcatgc ggcaacatgc ggtccaggcc aaagtagcca agcagaccga
     2461 agataaacat tccataaccc aacaccggcg acattccttc agctgctagt gcggcaggaa
     2521 gcatttccat taatgagatg agcaacatga tccccgccgc aaaacctagc gaaaacgcca
     2581 gtaagcggtt cgagggtttt tgtccgagaa cgccgagaaa cgcgccaata aacgtggctg
     2641 cccccgccag tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca
     2701 ccttcaggca gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact
     2761 ccgccgaaca agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca
     2821 tgtcttcaac acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg
     2881 aagataattt gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag
     2941 cgattgtggt ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga
     3001 cgccgcccac gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc
     3061 ctgctccggg ttcgcctgcg ctggcacagc gtctggttga gctgttagcg ccgatcccgg
     3121 tgacgctgga taaagaagcc tggggctttg accacggctc gtggggcgtg ctgattaaga
     3181 tgtatcctga cgccgatatc cctatggtgc agttgagtat cgacagtagc aaacctgccg
     3241 cctggcattt cgaaatgggg cgcaaactcg cggcgttgcg tgatgaagga ataatgttgg
     3301 tcgccagcgg taacgtggtg cataacctgc gcacagtgaa gtggcacggt gatagttcac
     3361 cgtatccgtg ggcgacgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggc
     3421 cggtagagca gcatcctctg gtgaattatc tcgaccatga aggtggcgcg ttatcgaacc
     3481 caacgccaga gcactatctg ccgttgttgt atgtgttagg tgtgtgggat gggcaggagc
     3541 caattaccat tccggtcgat ggtatagaaa tgggcagttt aagtatgctg tcggtgcaga
     3601 taggctaaca attaccggat ggcgagtgcc atccggtatc gtggcttatt caacaaaaat
     3661 atgtggataa aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat
     3721 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
     3781 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
     3841 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
     3901 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag
     3961 gttcgggtga ttcgggaaca tctcccacag tagcggtaga agtgccttgt tggagataat
     4021 actcttccac gccggttcca gccagcgtac gccagcatct tccagcttgg tggagaacat
     4081 ttcgcgcagc ataaattccc acggataaag cttgaacagg ttagaaatca cctgatcctg
     4141 taaatccgtg aactggcctt tttcgcctaa acctatctcg tcgatgtaga ggaactcggt
     4201 ggcgatttca gcttcggttg cgcagtcctg caaatactga atggttccgc gatcttccac
     4261 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca cactgttcac gcagctcaac
     4321 gaagcgatcg atcagttttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
     4381 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
     4441 cgttggcgta tcggcgttat tttccagaag tttaggttcg ccagtgccat cccacgccag
     4501 atcaagacgc gaataaagcg atggctggtg agtcagccat gattgacgca caaaactcca
     4561 ggtgtgtttt ggtatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     4621 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     4681 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     4741 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cggtcggtaa tactgactct
     4801 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     4861 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     4921 ggttttggtg ccattgccgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     4981 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg gactggccgg gtttttcgag
     5041 gagaacagcg gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa
     5101 ccggccatca gcggcatcca gaaactcccg ctggattgct gagcctgcgc ctggttttct
     5161 ggtgccatgc cagcctgggc tggtgcttgc tgacactgac cttcaccaaa ttcagcaaca
     5221 cagtcttcac gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     5281 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     5341 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     5401 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     5461 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     5521 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     5581 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     5641 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     5701 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     5761 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag caaatcctgc
     5821 tcgttcaacg tacccagggc tgacttaata ttcagctgat taatcaggta gttataacgc
     5881 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     5941 atggtacgcg taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgcgcggaa
     6001 actacggctt gtttgtaggc gttaatacta ctgatagaag cattaatgtt gttgaaggag
     6061 gaacgtacgg tttgcacgac gctacgatgt gcgctttcca gttgctcact ggcaccaaca
     6121 aagttgtact gtgcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     6181 gagaagctca ggccaacttt gttctggccc atattgctat cgtcatactg ggtaccagcg
     6241 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     6301 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     6361 ctcaagcatg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta
     6421 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag
     6481 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     6541 acttcgttcg ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct
     6601 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     6661 tgtgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     6721 gtgttgagga tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct
     6781 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     6841 agggacgcac tggtcgcgtt cgagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     6901 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     6961 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     7021 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc
     7081 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     7141 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     7201 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa catatgactg ctcaaacggc
     7261 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     7321 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     7381 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     7441 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     7501 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cggattgccg
     7561 cgtacgacac cagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     7621 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     7681 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     7741 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg
     7801 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     7861 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     7921 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     7981 tgcgcctgtg cgagatgaac ttttcataat tgcgccgttt gttaccgcgc aatgacgcac
     8041 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     8101 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     8161 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     8221 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     8281 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     8341 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     8401 gcctgttaac ccttcctctg gctggtgagg ccagaggaag gattttacaa attaccgata
     8461 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     8521 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     8581 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     8641 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     8701 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     8761 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     8821 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     8881 tgcatcatca tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg
     8941 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     9001 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     9061 cctgtgtgca gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct
     9121 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctagcgg
     9181 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     9241 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc
     9301 ggaacatcac cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc
     9361 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     9421 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     9481 ggtggtaaac ccggcggtac gcccgtttga attgctgacg gactatctcg gtcagaacga
     9541 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     9601 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     9661 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     9721 agaaggcggc aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     9781 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     9841 cttataacgc tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg
     9901 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     9961 tcgatgaagc actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt
    10021 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagaaggag
    10081 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
    10141 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtagttaac gccttgtcga
    10201 agcgcgtaga agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
    10261 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca
    10321 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
    10381 tgacgcatgt gctgaaggcc aaagcagtac tgtgccccgg cgttgagatt acctttaaag
    10441 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
    10501 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
    10561 gcgatactga agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga
    10621 ccgaaagcta cgtcaacctt atcccaacga tgcagggcgg tacccatgtt aatggtctgc
    10681 gtcagggcct gttggacgcg atgcgtgagt tctgtgaata ccgcaacatt ctgccgcgcg
    10741 gcgtgaagct gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggttaaaa
    10801 tgcaggatcc gcagtttgcc gggcagacca aagagcgtct ctcttcgcgt cagtgtgcgg
    10861 cattcgtttc gggcgtggtg aaagatgcct tcatcctgtg gctgaaccag aacgttcagg
    10921 cggcggagtt gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
    10981 aaaaagtggt gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt
    11041 gtaccgcgca ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg
    11101 gatctgccaa gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga
    11161 tccttaacac ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata
    11221 tttcggtagc gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca
    11281 aaatctgtat cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg
    11341 ctttgttcgt aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc
    11401 caccgctcta ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
    11461 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta
    11521 aaggtctggg ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc
    11581 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
    11641 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg
    11701 acatggcgga gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa
    11761 caaggcagga ttaaatccct ggctgcagaa tacggatatt catctccagc acgtcgcctt
    11821 ttacggcttt gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga
    11881 tgctttccca ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc
    11941 cagcagcgca atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg
    12001 gaacgatttt agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac
    12061 ggatttctgc gattacggta agcatggtta actccttcta aagcctgagc tctagttaac
    12121 caaaaatttc cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt
    12181 ttatcacgtc attagcgata aatgtcggca gcggttccat accgaggaat tggtttgctt
    12241 tatggaacgg cagatacaca ccgtcaacgc caacgccgtg gaagaactga tctttttcgg
    12301 tgaaggcttc cattggtgcg ttccaggtta gagaaagcat atattttttg ccctgtacca
    12361 ggccgccgga accgtatttt ttcgacggat ctttgcgggt acgaccatcg ctggcataca
    12421 gagtcccgtg accttcggtg aatacatcat caatatattt tttcactgtc cacggcgcgc
    12481 ccatccacca gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt
    12541 ccgctttgac attgtagtcg ctgtcggcgc gaacgatgcg gacatcatgc ccgaggtcgc
    12601 gcagtgtgcc atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga
    12661 attttttcgc gccgttgata atcaggatgt tgctcatttt ttaacctcaa ggtgagacgt
    12721 ttgtcgacta tagtacgtca cagagcggta cggtgaaatt agcaaaatgt gcaaagtctt
    12781 ttgcgaattt agcaaaagtc tgatctagct ctgactcacc gatgcaggat aaatattttc
    12841 tgaaaatgac aatactgata gccattcctc ataattctta ctcgtaccgg aataatttac
    12901 acgtttggtt cggttttttt ggggattgaa aatatggctt tgaataagta aaagatgaaa
    12961 atatcaacgt gtcatgttga taattataaa tggattttta tgagcgtact gatatatgtt
    13021 aaagcagaaa attaaaacaa tatttgaagc attgctatat ataatgctta cttattggtt
    13081 aatagattct ttttttgctt tcaataagta tgactggatg cttgagtcgg gaggcaatat
    13141 ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga ttgcagcatt
    13201 ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc gggaaatgtt
    13261 cgagttttgg ctatatgtat tctcgctcgt tatttgtctg gtttgtggtt ggtggctttt
    13321 ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt aacggctctc
    13381 atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc gcattcccaa
    13441 actcaacatt catgtcatgc agcttggcga ttcgctggac aatcgatagc ccaagtccac
    13501 tgccggtagc ggtttgtccg ggtgggcgat agaagcgttc gccaattcgc gccagtgcct
    13561 ctggtgtcac accggggccg ttatccctca cgatgaaatt atcagcattc agcgtgacgt
    13621 ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt cgcaccaaca
    13681 aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca
    13741 cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat
    13801 cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg
    13861 tgagcagttg atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg
    13921 cttttttccg cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca
    13981 gcgctgttaa cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa
    14041 ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt
    14101 cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg
    14161 ccagcgccag tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta
    14221 acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca
    14281 tgtcttcacg gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg
    14341 tcatccagac aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt
    14401 cccgttgata gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc
    14461 cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt
    14521 tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta
    14581 accgtttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt
    14641 gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta
    14701 gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta
    14761 gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac
    14821 atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat
    14881 cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc
    14941 ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag
    15001 catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc
    15061 ctccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc
    15121 cagacgcagc ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg
    15181 ctcacgctga cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa
    15241 ggttaaatcc aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg
    15301 accttgtgta aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc
    15361 accaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc
    15421 gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt
    15481 ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg
    15541 agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc
    15601 ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa gtcaggccgg
    15661 aggatttcag gggccgaacg gcagcgtaac gactgtagaa agcgtaaaat ccctgcgtga
    15721 cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt
    15781 gtttaaagat gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt
    15841 gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc
    15901 tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg
    15961 acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa
    16021 acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt
    16081 tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg
    16141 ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc gcaccggaag
    16201 cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg
    16261 tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg
    16321 acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga
    16381 aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc
    16441 agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt
    16501 gagccaaatc actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc
    16561 ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt
    16621 aagccgtctc gggggtatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg
    16681 gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt
    16741 aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc
    16801 cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa gtctacagca
    16861 aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg
    16921 ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat
    16981 tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga
    17041 gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat
    17101 catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt
    17161 gtatgcgatg tctgaactgg gcctgaatgc cagagccaaa tttaaaaaat cggcccgtac
    17221 tgtcggtgac gtactgggta aataccatcc gcacggcgat agcgcctgtt atgaagcgat
    17281 ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg
    17341 gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc
    17401 gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa
    17461 cttcgacggc actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct
    17521 taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg
    17581 tgaagtagct caggcggcaa tcgcattaat cgaccagccg aaaaccacgc tcgatcagct
    17641 gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc
    17701 cgagattcgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa
    17761 gaaagaagat ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt
    17821 actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg
    17881 cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt
    17941 ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg
    18001 cattaacctc aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat
    18061 cctctccgaa tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct
    18121 ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat
    18181 cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg
    18241 gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc
    18301 caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca
    18361 gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca
    18421 ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc
    18481 gaaagctatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca
    18541 gatgggctgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa
    18601 agcgggtgat agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt
    18661 tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca
    18721 gggcgaacca ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct
    18781 gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg
    18841 cacctttaac gatctagtgg cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga
    18901 aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat
    18961 cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg
    19021 caaaggcaac aagattatca acattccatc ggcagaagcc gcacgtggcg aggatggtct
    19081 ggcgcaactg tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa
    19141 aattaaactg cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac
    19201 gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag
    19261 cagcggtgat agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg
    19321 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    19381 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    19441 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    19501 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    19561 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    19621 gccacctttg ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc
    19681 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    19741 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    19801 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    19861 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    19921 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    19981 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    20041 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    20101 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    20161 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    20221 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    20281 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    20341 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    20401 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    20461 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    20521 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    20581 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    20641 ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    20701 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    20761 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    20821 atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca
    20881 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    20941 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    21001 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    21061 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    21121 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    21181 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    21241 ttcgcggctt ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc
    21301 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    21361 tcatggctgg ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    21421 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    21481 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga
    21541 tccgtaacgt gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    21601 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    21661 cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt
    21721 tggtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt
    21781 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    21841 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    21901 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    21961 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    22021 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt
    22081 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    22141 gaatctgttt tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc
    22201 cgatcgccgt ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc
    22261 ggatcgcgcc acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt
    22321 gatcctcggc ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga
    22381 taccgtgcgc cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg
    22441 tgaacgtccg ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat
    22501 ccgcgatgtg cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt
    22561 ggattccacc cgtcttgata ccccagggaa aatcgaccca atcccgcatc cgtatggcga
    22621 agatttgccg tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt
    22681 aatcgtgcag ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt
    22741 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga
    22801 aaccaaccca ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    22861 caacccgcct gctatcccgc tttctaccga agagatggac tgcgtttttg cgctgccgta
    22921 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    22981 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    23041 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    23101 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    23161 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    23221 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    23281 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg
    23341 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    23401 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    23461 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    23521 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    23581 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    23641 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    23701 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    23761 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca
    23821 ggcgctggaa gcgatgggca aaaagcatct gattggcagc cgtcgcgatt gcttagtgcc
    23881 tgcgccaacc attgaagaga tgcgtgaagc tcgtcgccag aaccgcaata cccgtccggc
    23941 gttgactaaa catacgccga tggcgactca gcgtcagacg cctgctacgg caaaaaaagc
    24001 gtcgtctacg caatctcgtc cggtgaatgc tggtgcgaag aaacgcccta aagcggcggt
    24061 tggacgttaa aaaaaatccc cggcaactga catgctgccg gggatttttt tatcattctg
    24121 cgtacatacg caagcagcgc aggcaatcaa aatagtcctg tgcatcaaga cgcgtattgg
    24181 ctctgttagc ttcggatttg gcagcataga tttgtgggcg tagtgctttc atatcctgct
    24241 caagccgggc gattttggca tttaatgttt gtacattgct cgccagttga ttgacctttt
    24301 gctcattgac gcagccactg agcaagcagg ttgccagaac aacagctccc acggtgaaaa
    24361 tcgttttcat atagtgttcc cttttattta ttgttccggc taattgttat ttaattcagc
    24421 tcattgcttt aatctggcgg cggtttttgc ctcaatcagc ttaagtaggc tgccgctgtt
    24481 cgggcggata aaatcagcga tttcccactt accttcgcgc cgctgtaaaa ccagctcttg
    24541 ctcttcattg ttgccattgg tcagcgtaaa acgaacatga atgcgggcgg aatcggcggt
    24601 tttctccagg actcgaattt catcaacgcg tgaaaatagc gactcttcgc caaaacagat
    24661 cggattgtcg taaataaatt gccgttctat ttgcatatct tcatcgggat tattggccgt
    24721 cttgaaggtg gttttataga aggcattaac cgcctggcgg taagagtcgg aaaccaccca
    24781 cgtccacggc cagctataat cagcaatatg ttcaaagagt tcagcaacaa aggattctgg
    24841 ctgttctttc tgcaacgaag ctaaagcggc cagcgttttt tcattctcgc tattaactgc
    24901 ttgtaagacg ctgccatgat tgctgacaat atcatcaatg acccaacgac cattttccgc
    24961 caccattttc agtgtctgtg tggtcttttc tttatcatct ttaaagatac gaaagcgcac
    25021 aacggcatcg gcatgatcga cgtcagtttg ggttatcgct acgctttcta acaccagatc
    25081 gccaaaatcc tgacaatcac aaaccggatc ataatccagc cagccaatat tgcccggtag
    25141 tgtaagattg ttgttcaggg taagcgcctg ttgaatacgc gctgaagtta tcgcccgctc
    25201 tccggtttca ccgaaataag gcgtactggc atctgattta tagttctgat aaatctgccg
    25261 gactgtttgt tctacggtct gtgaaggagg ctgcgcgtgt acggtaaaac ttgccagggc
    25321 tgctaaaaac agaagtatta ttttcatctg tttttcacct ctccgttgcg tttatcccgg
    25381 atatagatga gttgttcttt taagagcttc cggctctgca tgatgatgtc cttatatttg
    25441 gcattcctga acaaggaaga gtaacaacgg gcgggacgcg aggggaataa atgatttctg
    25501 aaaagtccgg tagcggaaca ttaccgccac cgggagaatt tgcacgttag ccgccgaact
    25561 ggtcaggatc gggaccgaga cgcttgccct gatcgagttt tgcaattttg ccgagttcgt
    25621 ctttgtcgag acggaaatcc cagacatcaa agttttcggc aatacgtgaa ggtgtgaccg
    25681 atttcgggat caccaccagg ccgctatcca gatgccagcg gataacaatc tgcgccgggg
    25741 ttttgccgta tttatctgcc agatcgcgaa tgactttctg atcgaaaacg ccttttcctc
    25801 cttgcgctaa tgggctccag gattcggtct ggattttgtg tgtcgcgttc caggcgtgta
    25861 gctggcgttg ttgcatcagc ggatgaagtt cgatctggtt tatcacaggc gtcacgccag
    25921 tttcatcaat caggcgttgc aggtgatgga tctggaagtt gcacacgccg atgcttttga
    25981 ttaatccctc tttttgcaat tcgatcatgc ctttccatgc ttcgacataa tggtcgatag
    26041 cgggaacggg ccagtgcatt aagtagaggt cgatataatc aagctggagt tttttcaggc
    26101 tgtcgagcag ggcttcgcgg gggcacttgt ggtcgtcgtt ccacagctta gtggtgatga
    26161 acagttcttc tctgttgact gaggcatttt tcagggcttt gccgacacct tcttcgttct
    26221 tgtaggccgc ggcggtatca atcgagcgat aacccacttc taacgctttt tgaatggcgg
    26281 tgattacttc ctcattactt gcttgccaga cgcccagtcc cagctggggc atgacattgc
    26341 catcctgtag cttaataacg gttggattag ccatacgttc ctcctttata tgaactcacc
    26401 ggagcaggct ccggtgaggt gttgtgaact taagtctgga cgaaatgccc gaaaacgaaa
    26461 gtttgaggcg taaaaagctt agcgggcggc tttgtatata cggcggctga catccaacgt
    26521 aatgtcatga ttttcgccca gttgggtcat gccgtgctct tccagttttt tcagcaaagc
    26581 cgggatggag ctgccgtcca gaccgtagtc ggagaggtgg gtcggcacgc ctaattgctc
    26641 aaagaaattg cgggttgcgg caatcgcggc gtcaatacgc tcatcgtcgg aaccttcagt
    26701 gatgttccag acgcgttcag catattgcag cagcttagcg cgcttggtct cgcgtttttc
    26761 attccacagt gcaggcagga cgatagccag tgtttgcgcg tgatccagac cgtgcatcgc
    26821 agtcagttcg tggcccagca tatgcgttgc ccagtcctgc ggtacgccag cgccaatcaa
    26881 accgttcagc gcctgagtcg ccgcccacat gacgttggcg cgcacatcgt agttttctgg
    26941 ctctttcagg gctttcggac catcttcgat tagcgtcagc aaaatgcctt ctgcgaaacg
    27001 gtcctgaatt ttggcatcaa ccggtttggt aacatactgt tccacggtgt gtacaaaggc
    27061 gtccactacg ccgttagcca cctgacgcgg cggcagggtg taggtataaa ccggatcgag
    27121 cacggcaaat accggctgaa catgtgcaga atggaacgcc tgcttgtcgc ctgtggtttt
    27181 acgggagatc accgcgcctg cgttggattc tgaaccggtt gctggcagcg tcagcacaca
    27241 gcccatcggg atggcgcttt taatctcttt accgcccgtt tgcagaatgt gccacggatc
    27301 gatattttcc ggatagttag ccgctgcggc gataaatttg gtgccgtcca gtacagaacc
    27361 gccgccaacc gccagcagga aagtcacttt ctgttcgcga accagtttca cggcgttcat
    27421 cagcgtttca taagccgggt ttggctcaat accgccaaat tccagcacgt ccatgccttt
    27481 cagggcatcc agaacttgat cgagaacgcc ggtttttttc acgctgccgc caccgtaggt
    27541 aatcaatacg cgagcatcgt gaggaatttg ttcgcgtaaa ccagcgattg cgcctttacc
    27601 aaacagaatg cgggttgggg tgtgcagatt aaagttgttc attacttgct ccctttgctg
    27661 ggccaatatg agggcagaga acgatctgcc tgatgttttt cattgtgatc gccagcgccc
    27721 tagctctcaa tgctcatttc tgccaatgtc ttgcctattt ctccagagtg ctggagaaat
    27781 gctacaaaat tgcgcacaat caaattgccg cattattcct aagaaattac gcgatatgaa
    27841 acgtgaagag atttgtcgct tgctggcgga taaagttaat aaactgaaaa ataaagaaaa
    27901 tagtttgtca gaactgttgc ccgatgtgcg tttgttgtat ggcgagacgc ctttcgcacg
    27961 tacaccggtg atgtacgagc ctggcatcat aattctcttt tccgggcata aaatcggtta
    28021 tatcaat
//
LOCUS       DADLTI010000039        27738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLTI010000039 DADLTI010000000
VERSION     DADLTI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.039"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1100)
                     /locus_tag="JN755_001524"
     CDS             complement(<1..1100)
                     /locus_tag="JN755_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY7380364.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN755_001525"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN755_001525"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7380365.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN755_001526"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN755_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7380366.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN755_001527"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN755_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7380367.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN755_001528"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN755_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7380368.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN755_001529"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN755_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7380369.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="JN755_001530"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="JN755_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7380370.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN755_001531"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN755_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7380371.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /locus_tag="JN755_001532"
     CDS             complement(5804..7084)
                     /locus_tag="JN755_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7380372.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JN755_001533"
                     /pseudo
     CDS             complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JN755_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JN755_001534"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JN755_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7380373.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JN755_001535"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JN755_001535"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7380374.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /locus_tag="JN755_001536"
     CDS             10284..11702
                     /locus_tag="JN755_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7380375.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="JN755_001537"
     CDS             11851..12015
                     /locus_tag="JN755_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380376.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="JN755_001538"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="JN755_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7380377.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="JN755_001539"
     CDS             13524..13769
                     /locus_tag="JN755_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7380378.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="JN755_001540"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="JN755_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7380379.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="JN755_001541"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="JN755_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7380380.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="JN755_001542"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="JN755_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7380381.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="JN755_001543"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="JN755_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7380382.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="JN755_001544"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="JN755_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7380383.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="JN755_001545"
     CDS             19432..19785
                     /locus_tag="JN755_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7380384.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFSDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="JN755_001546"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="JN755_001546"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7380385.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="JN755_001547"
     CDS             21087..22214
                     /locus_tag="JN755_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7380386.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /locus_tag="JN755_001548"
     CDS             22282..24216
                     /locus_tag="JN755_001548"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7380387.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="JN755_001549"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="JN755_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..26424
                     /gene="pdeR"
                     /locus_tag="JN755_001550"
                     /pseudo
     CDS             24450..26424
                     /gene="pdeR"
                     /locus_tag="JN755_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26572..26745
                     /locus_tag="JN755_001551"
     CDS             26572..26745
                     /locus_tag="JN755_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380388.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="JN755_001552"
     CDS             26835..27584
                     /locus_tag="JN755_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7380389.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6433 a         7193 c         7124 g         6988 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtatcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgagg cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt
     9001 gacaccccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactaa atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctggtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtacctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttgacgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcgatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttagt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggcgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acgtgcgt
//
LOCUS       DADLTI010000040        27536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLTI010000040 DADLTI010000000
VERSION     DADLTI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.040"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN755_001553"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN755_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7380390.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN755_001554"
     CDS             complement(1614..1958)
                     /locus_tag="JN755_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7380391.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN755_001555"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN755_001555"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7380392.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN755_001556"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN755_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7380393.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN755_001557"
     CDS             complement(6144..6842)
                     /locus_tag="JN755_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7380394.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN755_001558"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN755_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7380395.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN755_001559"
     CDS             7537..8574
                     /locus_tag="JN755_001559"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7380396.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN755_001560"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN755_001560"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7380397.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN755_001561"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN755_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7380398.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN755_001562"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN755_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7380399.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN755_001563"
     CDS             10745..11626
                     /locus_tag="JN755_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7380400.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN755_001564"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN755_001564"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7380401.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JN755_001565"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JN755_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7380402.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN755_001566"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN755_001566"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7380403.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JN755_001567"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JN755_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7380404.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JN755_001568"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JN755_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7380405.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JN755_001569"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JN755_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7380406.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JN755_001570"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JN755_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7380407.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JN755_001571"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JN755_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7380408.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JN755_001572"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JN755_001572"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7380409.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JN755_001573"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JN755_001573"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7380410.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JN755_001574"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JN755_001574"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7380411.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JN755_001575"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JN755_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7380412.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JN755_001576"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JN755_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7380413.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN755_001577"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN755_001577"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7380414.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JN755_001578"
     CDS             24893..25273
                     /locus_tag="JN755_001578"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7380415.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JN755_001579"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JN755_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7380416.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN755_001580"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN755_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7380417.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="JN755_001581"
     CDS             26442..>27536
                     /locus_tag="JN755_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY7380418.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6793 a         6856 c         7221 g         6666 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgctag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgctg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DADLTI010000041        27079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLTI010000041 DADLTI010000000
VERSION     DADLTI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.041"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            231..1664
                     /locus_tag="JN755_001582"
     CDS             231..1664
                     /locus_tag="JN755_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY7380419.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JN755_001583"
     CDS             complement(1672..2730)
                     /locus_tag="JN755_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7380420.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JN755_001584"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JN755_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JN755_001585"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JN755_001585"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY7380421.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN755_001586"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN755_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7380422.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN755_001587"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN755_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7380423.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JN755_001588"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JN755_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7380424.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JN755_001589"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JN755_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7380425.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN755_001590"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN755_001590"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7380426.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN755_001591"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN755_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7380427.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN755_001592"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN755_001592"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7380428.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN755_001593"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN755_001593"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7380429.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JN755_001594"
     CDS             complement(17699..18322)
                     /locus_tag="JN755_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7380430.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JN755_001595"
     CDS             18619..18894
                     /locus_tag="JN755_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380431.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JN755_001596"
     CDS             complement(18971..19213)
                     /locus_tag="JN755_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7380432.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JN755_001597"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JN755_001597"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7380433.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JN755_001598"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JN755_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7380434.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN755_001599"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN755_001599"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7380435.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /locus_tag="JN755_001600"
     CDS             22592..24289
                     /locus_tag="JN755_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7380436.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..24699
                     /locus_tag="JN755_001601"
     CDS             24304..24699
                     /locus_tag="JN755_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7380437.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSVRLPRR"
     gene            24657..25037
                     /locus_tag="JN755_001602"
     CDS             24657..25037
                     /locus_tag="JN755_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAY7380438.1"
                     /translation="MATTADQRTPAATVTRENDTINLVKDERIIVTPINDIYYAEAHE
                     KMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYILR
                     LKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JN755_001603"
     CDS             25050..25907
                     /locus_tag="JN755_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7380439.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..>27079)
                     /locus_tag="JN755_001604"
     CDS             complement(25910..>27079)
                     /locus_tag="JN755_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7380440.1"
                     /translation="MDIGGDKSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAI
                     LRAASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSV
                     CYIIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAH
                     QRGKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQ
                     ACECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRT
                     EHPFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEV
                     ELVIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
BASE COUNT         7451 a         6417 c         6053 g         7158 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgtggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccat
//
LOCUS       DADLTI010000042        26318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLTI010000042 DADLTI010000000
VERSION     DADLTI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.042"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            109..1092
                     /locus_tag="JN755_001605"
     CDS             109..1092
                     /locus_tag="JN755_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7380441.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1115..2626
                     /locus_tag="JN755_001606"
     CDS             1115..2626
                     /locus_tag="JN755_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7380442.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2651..3649
                     /locus_tag="JN755_001607"
     CDS             2651..3649
                     /locus_tag="JN755_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7380443.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3682..4776
                     /locus_tag="JN755_001608"
     CDS             3682..4776
                     /locus_tag="JN755_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7380444.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4788..5660
                     /locus_tag="JN755_001609"
     CDS             4788..5660
                     /locus_tag="JN755_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7380445.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5708..6130)
                     /locus_tag="JN755_001610"
     CDS             complement(5708..6130)
                     /locus_tag="JN755_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7380446.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6227..7429)
                     /locus_tag="JN755_001611"
                     /pseudo
     CDS             complement(6227..7429)
                     /locus_tag="JN755_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7439..8251)
                     /gene="hcaB"
                     /locus_tag="JN755_001612"
     CDS             complement(7439..8251)
                     /gene="hcaB"
                     /locus_tag="JN755_001612"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7380447.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8248..8568)
                     /locus_tag="JN755_001613"
     CDS             complement(8248..8568)
                     /locus_tag="JN755_001613"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7380448.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8568..9086)
                     /locus_tag="JN755_001614"
     CDS             complement(8568..9086)
                     /locus_tag="JN755_001614"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7380449.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9083..10444)
                     /locus_tag="JN755_001615"
     CDS             complement(9083..10444)
                     /locus_tag="JN755_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7380450.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10580..11470
                     /gene="hcaR"
                     /locus_tag="JN755_001616"
     CDS             10580..11470
                     /gene="hcaR"
                     /locus_tag="JN755_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7380451.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11630..12769
                     /locus_tag="JN755_001617"
     CDS             11630..12769
                     /locus_tag="JN755_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7380452.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12761..14041)
                     /gene="csiE"
                     /locus_tag="JN755_001618"
     CDS             complement(12761..14041)
                     /gene="csiE"
                     /locus_tag="JN755_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7380453.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14232..15086)
                     /locus_tag="JN755_001619"
     CDS             complement(14232..15086)
                     /locus_tag="JN755_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7380454.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15231..16034)
                     /gene="suhB"
                     /locus_tag="JN755_001620"
     CDS             complement(15231..16034)
                     /gene="suhB"
                     /locus_tag="JN755_001620"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7380455.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16153..16893
                     /gene="trmJ"
                     /locus_tag="JN755_001621"
     CDS             16153..16893
                     /gene="trmJ"
                     /locus_tag="JN755_001621"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7380456.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17163..17651
                     /gene="iscR"
                     /locus_tag="JN755_001622"
     CDS             17163..17651
                     /gene="iscR"
                     /locus_tag="JN755_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7380457.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17763..18977
                     /gene="iscS"
                     /locus_tag="JN755_001623"
     CDS             17763..18977
                     /gene="iscS"
                     /locus_tag="JN755_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7380458.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19005..19391
                     /gene="iscU"
                     /locus_tag="JN755_001624"
     CDS             19005..19391
                     /gene="iscU"
                     /locus_tag="JN755_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7380459.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19408..19731
                     /gene="iscA"
                     /locus_tag="JN755_001625"
     CDS             19408..19731
                     /gene="iscA"
                     /locus_tag="JN755_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7380460.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19827..20342
                     /gene="hscB"
                     /locus_tag="JN755_001626"
     CDS             19827..20342
                     /gene="hscB"
                     /locus_tag="JN755_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7380461.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20359..22209
                     /gene="hscA"
                     /locus_tag="JN755_001627"
     CDS             20359..22209
                     /gene="hscA"
                     /locus_tag="JN755_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7380462.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22211..22546
                     /gene="fdx"
                     /locus_tag="JN755_001628"
     CDS             22211..22546
                     /gene="fdx"
                     /locus_tag="JN755_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7380463.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22558..22758
                     /gene="iscX"
                     /locus_tag="JN755_001629"
     CDS             22558..22758
                     /gene="iscX"
                     /locus_tag="JN755_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7380464.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22817..24100
                     /gene="pepB"
                     /locus_tag="JN755_001630"
     CDS             22817..24100
                     /gene="pepB"
                     /locus_tag="JN755_001630"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7380465.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24242..25018
                     /gene="sseB"
                     /locus_tag="JN755_001631"
     CDS             24242..25018
                     /gene="sseB"
                     /locus_tag="JN755_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7380466.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25839..>26318)
                     /gene="sseA"
                     /locus_tag="JN755_001632"
     CDS             complement(25839..>26318)
                     /gene="sseA"
                     /locus_tag="JN755_001632"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7380467.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6245 a         6558 c         7283 g         6232 t
ORIGIN      
        1 tacgttcact aaaccctata aactgtaata tttgagcggc acgcgctgcc gctcatcaca
       61 aaataattag aataatccgt tgttaataaa aaccctacag gaggcactat gcctaaaaaa
      121 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
      181 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccca gggatattac
      241 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
      301 attgaaacta acgcccaggg ggatatttcg aaagaaagta cctttgttga taccctcgtg
      361 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
      421 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
      481 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
      541 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
      601 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
      661 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta accaagaagg gactgtttta
      721 gataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
      781 atgggggagt cgggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
      841 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
      901 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
      961 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
     1021 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
     1081 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
     1141 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
     1201 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
     1261 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
     1321 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
     1381 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaatct
     1441 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
     1501 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
     1561 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
     1621 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
     1681 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
     1741 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
     1801 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
     1861 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
     1921 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgctac gtcgtggcga
     1981 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
     2041 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcatgcgccc
     2101 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
     2161 ggggatcatt ccctggctgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
     2221 cagcaccaac ggtgtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
     2281 gcggatgacg gtcaaggctg ccagtagcga aacacccatc ggcacgcttt ctggtggcaa
     2341 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
     2401 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
     2461 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg aattgccgct
     2521 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
     2581 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
     2641 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
     2701 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
     2761 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
     2821 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
     2881 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
     2941 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
     3001 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
     3061 actggggctg tggagcgctc tgcgcggaat ggggctgttt atgaccaacg cacagccagt
     3121 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
     3181 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
     3241 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
     3301 taacgttcgt cgagtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
     3361 cggcattttg ttggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
     3421 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
     3481 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
     3541 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
     3601 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
     3661 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
     3721 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
     3781 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
     3841 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
     3901 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
     3961 taccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
     4021 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
     4081 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
     4141 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
     4201 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
     4261 ctgggggcgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
     4321 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggggatggat
     4381 cacggctatt tagcaaccac cgaaggtctg ccgcagataa ttgctgaact tacccacggt
     4441 ggcgcggatg ttgcgctcga ttgttccggt aatgccgcag gtcgcttgct ggcactgcaa
     4501 tccactgctg actgggggcg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
     4561 gtcagcgctg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
     4621 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
     4681 attacccatc gcttctcgct agaacaggca ggtgatgcct atgtgctgat ggcgagcggc
     4741 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
     4801 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
     4861 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
     4921 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
     4981 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
     5041 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
     5101 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
     5161 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
     5221 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
     5281 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
     5341 aactggattt taaccagctc gcgccgttgc cgcgtcagtg ggtgaacaat ggttttgccg
     5401 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
     5461 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
     5521 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
     5581 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacctca gagatgtcgt
     5641 tgcgggtaga gtggctgtaa tttataaaat gccggataag gcgttcacgc cgcatccggc
     5701 atctttccta acgccgatcg agagaaattg cccccggtcc ggtaattgcc aatagcaaga
     5761 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
     5821 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
     5881 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
     5941 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
     6001 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
     6061 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgta
     6121 gtgtgttcat aaccaatccc tttgcaagat aatgtgattt aaacgtaata cacactcttg
     6181 cacctcgcca cgccccaggc attgagatat tcgctaatta tcctggttac agtgatttaa
     6241 gcgcgatgtt ctcatctatc agcagtttcg catcaaacgt tttgccgctc tggatccatt
     6301 tgcgaattgg gcgaatctca cgcccctgat tcagcgtgac cgcaccgata agcacgccgt
     6361 tttgcagatt aaaccaaatc gccttctgag tttctgggtt gccacgacaa agctagtcat
     6421 cgccacgcat atcgccaata aactgtaagt tatcactgta ctgatcgctc cagaaccacg
     6481 gcggcggcag tagcggtagc ggtagcccta acattgcggc agcggcaatt tgcgcctggt
     6541 tattggcgtt ttcccagctt tcgcagcggt gtagtgcacc attatcaaga cgagtgattg
     6601 ccacatcgcc accggcaaag atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa
     6661 tgccattggc agtatcaagg ttggcctcgc gagccagttg ctcgttggcg ctgatactaa
     6721 taccgtaaat caccacatca gcctgaagcg tttccccact ttgcagcgtc agttctactt
     6781 tttcaccatc gaccacatgt tcaatggcat tattgagcag aatgcgcaca ccagcctgct
     6841 gatggcgctg taaaagatag cgttgcacgg gcggtggtgc attacggccc atgacggttg
     6901 ccgccagttc aatcactgtc accttacatc tgcgctgcgt ggcgctggca gccagttcca
     6961 gaccaatagt tccggcaccg acaatcacga ctgaccgttc gggctgcaga acttctcgca
     7021 gtctggcggc atcaccggca tggtgtaggg taaagcagcg ttctcccagt gcatcaagca
     7081 acggcagcgg tcgagctgcc gcgccggttg ctataaaaag ctgatcccag tgccagcttt
     7141 cgccgttggt taacactaac tctcgtgtgt cgcgacccag cgttttgatg gttacaccgg
     7201 aatgcagatg aacattgttt tcctgccacc agttagcggg taacacctgc tgtaactgcg
     7261 gggaatcttc cagcaacatg gatttcgaga gtggaggtcg ttcataagga agatgtcgct
     7321 catcggaaaa cagatgcagc tcaccggtga acccttgctg gcgtagcgag gccgcagcca
     7381 ttgccgccgc ttgcccgcca ccgacaataa tgatcgtttt ttctttcatc gtgcttcctt
     7441 aaagatccag cccagccgct acgtggcgaa tgccgcgaat cgctaaaccc gcatcagcgt
     7501 tgatcatcac accgcttaat gcgcgattat tgcgccgcga tgccaacatc acatacggcc
     7561 ccgtaaaatc cgccggttgc gggaaaaatt gcagcggtaa aatggcggca attttctccg
     7621 gcgtcagaga ctgcattatc gaggtttcac tttgcccgag cgcctgtggg ccgcgcaggt
     7681 cgctggccat accacacggg ccgacgccat tcacccgcac tttcggtgcc agttcataag
     7741 ccagttggcg aataagtccg gttgcggcat gtttactggt ggtgtacagc gggccaccgc
     7801 cgccaggata ccaggcggca tttgacagtg tgaaaatcat gctgccttca ctggcgatta
     7861 acgccggagc gcaggctttt gcgcccagca ggtaaccgag tacgttgacg ttaaacagct
     7921 cgtggaagcc ggtttcgagc gtctctgcgg gagtattaac cagtgaggca ttgtgatccc
     7981 agatgcctgc attgccgata aaacaatcca gcttgccgga acgagtcagg atctgatcga
     8041 ccgcgcgttg ataatcggca taacaggtca cgttaccttc caccgccaga atatgttcgc
     8101 caaatcgctg acgcagactg gcgacttttg ccgccgacag ttccagcgcg gcaacctgcg
     8161 cgccttcttc gataaatcgc tcgaccagcg ccagccctaa tcccgatccg ccgccggtaa
     8221 taaaaatgga ctcgttatgc agatcgctca tggctgcgcc tccggtaagt cgatgaaaat
     8281 gtcaccacct tcaacgtgta ctggataagt ggtgagcgga tcggtggcgg gcaggcataa
     8341 cgctttcccc gttttcaggc aaaaactggc ggcgtgtagc gggcactcca ccgtggcgtc
     8401 atcttccaga tacccttctg acatcgacgc attaccatgg ctgcaacgat cgttaatggc
     8461 ataaaactcg ccgcccacgt tgaacaaggc gatgacgggc gaggtatcta tccggagagc
     8521 ctcaccctcc ggcacatccg caacgggaca cgcataaatt cgattcatca gaacagtaca
     8581 ctcaggttat gggaagtgat caccgcttga tccaggacga tatcccgttc cagcaagcgc
     8641 cagttgtcat cttccagacg gcgaactttg tcgaaacgcg ttccaacata gaatgtttca
     8701 tcgcgctctt tttgtgcccg ataaagtaga taattcaccc tcacggcaaa tacgtttggg
     8761 atgtcggttt cgcttacctg gcagttgctg attaagtgac gagtgcgtga cggcggctct
     8821 tctgcccagg ccatgcccgt ttccagacgg gcgattcgcc gctccagctg gtctttggtg
     8881 tcattgaaaa tccaggtcgt cggcggctgg acgcctttgc gacggtcgcg tgtttgcgcg
     8941 ttaaccgtgg tgcgcatggt gtaacgaatc tcttcgtcga gctgtgccaa ccagtcacga
     9001 aatttccagt catccagcag actggcttcg tgaaagagaa actggctaat gcggtgatgt
     9061 aactccagtg aaacttgcgc actcatttca tcacctcctg ctggtaagcg gcggttttat
     9121 cgagcacttc ctgccagctt tcgctactca gaagatcggc ccagcgttgg tacattccac
     9181 gagcggcagt ttctgaaaag atatagttag taatgccagg aatgccgtcg tcgcggcgct
     9241 tttcctgacc aagccccatt tccagacaca gtttgctgtt gcgggcgcgg tggcctttaa
     9301 gcaatttctg gatttcacac cagttctccg agtcatcctg ctcgagaaaa ccagcaggac
     9361 caaaagcacg agtggcgctg ttttcaaaag cggctttaac ttcatcggag gcggctttgt
     9421 cagtaataca gaacgcccac acttcaactt gatcagggcc gcgcggatgc cagacgcgga
     9481 gtgtggcagt gccgtttagc catgaaagcg tggggaatat attgttatga cccgccaggc
     9541 gcagggcgcg aacttcacct aaacgttgtt ctgcttcggc ataggtttcg cggtaatagc
     9601 ttgaaactgc gccatcgacc cagacattag catccggttt ttcagtaaag aagaaaccgc
     9661 taccgtgacc gtcctgacca aattgcagcg catctttggc ggtttcccac accgggcggg
     9721 cggtttgtcc atcaccgagg cgcttatcgc tgccatcatc tttcgcccct aatacctgaa
     9781 cggcagaagc atggctgaac agagcatgat actggtcact ggcgaactgc tctgccggga
     9841 atttccagtt acagttgatc acccactttt gtacgccgcc gacaatttcg gtgccgcctt
     9901 cgcgacgatc cagcatgcca tccagatacc aggcaatgtc acccaggtaa tcatgcaggc
     9961 ccggtgcgct ggtatcccag ttgccaaaaa tcagtccttt ataactctcc acacaaggaa
    10021 cttcgtttag cccccagtgg gatttacaca acccttgtgg gtaggcgcga ggttccagcg
    10081 gtacatcgat caactcgccg ttaatgccat aagaccagcc gtgatacggg caggtaaagg
    10141 cgcgagtgtt gccacaatct gcataactca cacgcatggc ccggtggcgg cattggttaa
    10201 gaaacgcctt gatgctgccg tctttctggc gcaccacgac aactgcatct tctcccatgt
    10261 aggtgttaaa gaaatcaccg ggttttggga tctggctttc gtgggcgaga aatagccagc
    10321 aacgaccgaa aatacgctca agctccagtt ggtaaatgtc cgggtcggta taaatacgcg
    10381 gagtgacccg accgttttgg gtgtcaatca gttggtaaat gttcaaatct gagggtgtgg
    10441 tcatattttt taatcctgaa agataagatg tattgacctt ctatttttgt gccataagga
    10501 tgttgctggt tggttgctaa tatgtgaaat agttaatttc taccagctga gacgtttttt
    10561 cgataacaag gggaaggtga tggaactacg gcatttacgc tatttcgtcg cagtggcgca
    10621 ggcactgaac tttacccgtg cggcggaaaa actgcatacc tcacagcctt cgttaagcag
    10681 ccagatccgc gatcttgaaa actgtgtcgg tgttccgtta ctggtgaggg ataagcgcaa
    10741 agtcgcgttg acggcggcgg gagaatgttt tctccaggac gcgctggcaa tcctcgaaca
    10801 agcggaaaat gccaaattac gggcgcggaa aattgttcag gaagacagac aattaaccat
    10861 tggctttgtg ccatcggcgg aagtgaattt attgccaaaa gtattaccga tgtttcgtct
    10921 cagacagcca gacaccttga ttgagctggt gagtttaatc accacgcaac aggaggaaaa
    10981 aattcgccgt ggtgaactcg acgtcggctt aatgcgccat cctgtttata gcccggagat
    11041 tgattatctg gagctttttg acgaaccatt agtggtggtg ttaccggttg atcacccttt
    11101 agcacatgaa aaagagatca ccgccgcgca actggatggc gtgaatttcg tcagtaccga
    11161 tccggcgtat tccggttcgc ttgcgccgat cgttaaagcg tggtttgcgc aagaaaacag
    11221 ccagccaaat atcgtccagg tggcaacgaa tattctggtg accatgaatc tggtgggcat
    11281 ggggctgggc gtcactttga tacccggtta tatgaataat tttaataccg ggcaggttgt
    11341 ttttcgtccc attgccggaa atgtgccttc cattgcttta ctgatggcgt ggaagaaagg
    11401 ggagatgaag ccggcattac gcgatttcat cgccattgtg caggaacgtt tggcaagcgt
    11461 aacggcataa ttacgcatta tttttgcaac atgcgccgcg gatcgcagtt ttttagtgct
    11521 tgtgaactgg cgttacagac atcgggaagc aggctcgaat attcttattt ttcaacttgc
    11581 accaacattc gattaacccc agactaacgc ctcctgacgg gagggactca tggttttgca
    11641 atccacgcgc tggttggcgc tcggctattt cacatacttt tttagttacg gcattttttt
    11701 acctttctgg agcgtctggc ttaaagggat tggtttaacg ccagaaacca tcggcctgtt
    11761 attgggggca ggtctggttg cccgttttct cgggagtttg ctcatcgcgc cccgcgtcag
    11821 cgatccttcc cgcctgattt ccgccttgcg cgtgctggca ctgctgccac ttctctttgc
    11881 tgtcgccttc tgggcggggg cgcacgtagc gtggctgatg ctggtgatga ttggctttaa
    11941 cctctttttc tcaccgctgg taccgttgac cgatgcactg gcgaatacgt ggcaaaagca
    12001 gttcccgctt gattacggca aagtgcgact gtggggctcg gtggcgtttg tcattggctc
    12061 ggcgctgacg ggcaaactgg tcactatgtt tgattatcgg gtgatcctcg tgctgttgac
    12121 gttgggcgtg gcatccatgc tgctcggctt tctcatccgt ccgacgattc agccacaagg
    12181 ggcaagccgc cagcaggaga gcaccggttg gtctgcgtgg ttggcgctgg ttcgccagaa
    12241 ctggcgcttt ctggcctgcg tttgtttatt gcagggggca catgcggcct attacggttt
    12301 tagcgccatt tactggcagg cagctggcta ctcggcctcg gcggtggggt atttgtggtc
    12361 gctgggcgtg gtggcggaag tcattatctt tgcgctgagt aataaacttt tccgccgttg
    12421 tagtgctcgc gatatgctgt tgatctcggc gatttgcggc gtagtgcgct ggggcattat
    12481 gggagcaact acggcgttgc cgtggttgat agtggtgcaa attctgcatt gcggcacctt
    12541 cacggtctgc cacctggccg ccatgcgtta tattgctgct cgccaggtta gcgaagtcat
    12601 ccgtttacag gcggtttact ctgccgtcgc gatgggcggc agtatcgcta tcatgaccgt
    12661 tttcgccggt ttcctgtatc aatatctggg ccacggtgtg ttctgggtaa tggcgctggt
    12721 ggcgcttccg gcaatgtttt tgcgcccgaa agttgttccc tcatgctgat tcgagcattt
    12781 tgcggatctg ctcctgttga ggtgttgtca gcgtcaggtc cgtatagatc agcggtggtg
    12841 aaaagagcgg taacggcatg gtataaggcg caataatcag tgccgcgcca cgcggagcgc
    12901 ctgtctgcaa aaatgccttt accgacatat gcttaatgtt gagcggcagt aacgttagtt
    12961 cgcgtaactg ctgctcaatc tgcgcttctc gctcgctatc attcccggtc agtagaataa
    13021 tctgtttttc atgcagatcg ttttcctgca ttagccaggc accgaaaatc accgcgacca
    13081 gaccactttc ctcatcagaa agatggacgc cgtattcact ttcaaatccg gccagcgccg
    13141 cgcgggtgat gcgtaccagg cgtggataca gtctggcgaa ctcttccggc agggtattat
    13201 caataccgat ggcaaacaaa ctgcgattta acgcctgggc gaggtggata taaagctgat
    13261 cacataaccc ctgttcatca taaaaacgaa catttcccag ctcacgaaaa tggtttacca
    13321 gacgtttgat agactgacgc aattgtctgt cccgctgatg cgcatcccgc agtggatcgg
    13381 gaacccgcaa catggaaaaa agtaatgcca taaacagtgg ctcatcaggc ggtacatgct
    13441 ggagcgcccg acgctgccag tggcgtccaa tttcctgcgc tacctgaaat tcaaggcagg
    13501 attccgccca gcgacgttgg agcggattaa actgaggcgt aattccagcg tggtgctgca
    13561 gcaaacaata ttgcagatac agacacagga aatgaatatc gcgcgattca aagcgttttt
    13621 gcagccgtcg ggagcagaga ttcactaacg cttgtagatt ggtatcgtcg taaaagttac
    13681 gcgcaattcc gcgccgcttc agttcactct tcagggcggg ggtgaattgg ctggtaataa
    13741 atgacggaca cagacggaaa ccacgtcgta gccagtgaaa taaacacaaa cgttggttaa
    13801 gcactgtacc ttcaacccgg tagctaccgt cataaccagt cgtcagtgtg agttgatgat
    13861 agcgcaggat ctcccgtcct gtttcgctga tatcaagact ggcaatatca tcatccacac
    13921 cattaagctc gctgaaggtt gccatggtgg cggtcaaccc cggctgaaag agcgtcagca
    13981 agatctggca gcggcgctgg ggagccgaaa ggacagatgg tggagcaagc gtaggcatca
    14041 tctgtagatc tcccataagc ataattgata agaatagcta aagtttgctc agggaaatgg
    14101 cgaagggaat gctaatcatc agaaatgttg gcaagcgtca cagaattttt ctcattttaa
    14161 actgttctga ccagtggatt agcatgtctt aaggtggttt tgtgaatgat tatcgtgcta
    14221 gcgatcatgg gttagttctg cggattcaac gcgctaagga taaagtccac catctgttcg
    14281 cgataaacat tgccgtgacg atcggaaaaa gcatcaatgt gttcgccatc cgggattagg
    14341 atcaaccgtt ttggctcttt tgccaggcta tacaactttt cgctgtgctg ccatgggata
    14401 acgtgatcgg ctttaccgtg aatgagtaaa agcgggatcg ggctgacgct ggcgatgtaa
    14461 ttttcgccgc tgtaactctc atcaagtaag tagccactgc cggggatcat ctggttggcg
    14521 atggttgcat aagaggcaaa tgtggagtcg aggatcaccg cacgtatgcc ttcacgatca
    14581 ccctgaccaa taacagcaag aatattcgcc ccgccaatac tctgcccgaa cagcaccagc
    14641 cgttgtgggt ttacatcact gcgatggcgc accacattga tggcgctttg cgtatcgtcc
    14701 agcaatccgg cctgggacgg agtgcctttt gatttaccaa acccgcgata atcaaacata
    14761 aaaacgttga aattacgctc aggtaaccaa ctgaccagcg gccagtgggc ggacatattt
    14821 ccggcattgc cgtgagcatg aatgatggtt gcgatggcgt tgtcagcagg gcccgtcgaa
    14881 gaaggaataa accagccttg cagacgagta ccgtccttag ccgtaaattc gacggattcc
    14941 gccgaccagg ggtcgggacc gtaaatttta tcgtcaggat aatagaagaa gttgatggca
    15001 acgcgcagca ctaaataaac gcagaaggca acaacaaaga gaataaacag aattttggga
    15061 acgcgtttgt tcactggcag tgccataacc tttccttgcg acttctttat catccattaa
    15121 tcggggaaag atggtgggag agtgacgaaa gaaagtaaag cggtgaggaa gtatttcgga
    15181 atagcgcctg aaaggcgagg gcgggtgagt gatatcaccc gcctgagtca ttaacgcttc
    15241 agagcgtcgc ttaactcgtc acgcatgttc gccagtatgg ctttcacaac acgcgggtta
    15301 ccagcaacga tgttaccggt cagcatatag ttatgaccac cggtgaagtc gctgacgatg
    15361 ccgcccgctt cacgaaccag cagctcgcct gccgcgaaat cccacggacg cagaccgatt
    15421 tcaaagaaac cgtcaacacg acccgcagcg acgtaagcca gatccagcgc cgcagaaccg
    15481 gtacgacgga agtctgcaca ttcgttgaac agcttgccga cgatgttgat gtaggtagtg
    15541 gcgtactgtt ttgctttgaa cgggaagccg gtcgccagaa tggtaccgtc gagatcgcga
    15601 gcggtgctac cgcgcagacg gtagccgttc agctgtgcgc cctgaccgcg agtggcggtg
    15661 aacagttcgt tacgcatagg atcgtatacc acagcaactt cggtgcggcc tttgatacga
    15721 acggcgatag ataccgcgaa gtgcggcaga cgtttgataa agttggtagt accatccagt
    15781 ggatcgataa cccattgaac atcctgatca gtaccttcaa gttcaccgct ttcttcggtg
    15841 atgatggtgt gctgtgggta agatttacga atcgtgtcga taatcaccgc ttcggcagct
    15901 ttatctacgt tggtcacgaa atcgttactg cctttctggc tcgcttctac agcgtccggg
    15961 gtttcatagt ttttggcaat taaattaccc gccttgcgcg ctgcgcgcac ggcgatgttc
    16021 agcatcggat gcatcagtct ctctcactgg atgttaaaga acgggaaaac ggcgcagagt
    16081 atagcagcga gatcggcatc tgtcttcgtt ttatgataag atgcgtgaat aatctttaga
    16141 tgaagaaaga caatgctgca aaatattcga attgtgctgg tggagacgtc acacaccggc
    16201 aatatgggtt ctgttgcccg tgccatgaaa acaatgggat taaccaatct gtggctggtt
    16261 aatccactgg tgaaacccga ctcccaggcg attgccctgg ctgcaggggc cagcgatgtg
    16321 attggtaatg ctcacatcgt cgatacgctc gacgaagcgt tagccggttg tagcctggtg
    16381 gtgggcacca gcgcacgctc ccgcacgctg ccgtggccga tgctcgaccc gcgcgaatgc
    16441 ggtctgaaaa gcgtcgctga agcggcaaat accccggtgg cgctggtgtt tggtcgtgag
    16501 cgcgtcggct tgaccaatga agagttgcag aaatgccatt atcatgtcgc gattgcggca
    16561 aacccggaat acagttcgct gaacctggca atggcggttc aggttattgc ctatgaagtg
    16621 cgtatggcct ggctggcaac tcaggaaaat ggcgagcagg tcgagcatga agagacgccg
    16681 tatccgctgg tcgatgatct ggagcgtttt tatggtcatc tggaacaaac gctgctggca
    16741 accggtttta tccgtgaaaa ccatccgggg caggtgatga ataaattgcg ccgtctgttt
    16801 acccgtgcgc gcccggaaag ccaggaattg aatatcctgc gcgggattct ggcttctatt
    16861 gagcagcaga ataaaggtaa caaggccgaa taacagtggt tgcctgatgc gacgcttaac
    16921 gcgtcttatc aggcctacaa tgtacagaat cgtaggtcgg ataaggtgtt cacaccgcat
    16981 ccgacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa tacccgacta
    17041 aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga ccctgctatg
    17101 caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag gtgaagtaag
    17161 acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac gttgcgctca
    17221 actctgaagc gggcccggta ccgttggctg atatttccga acgtcaggga atttcccttt
    17281 cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc agcgtacgtg
    17341 gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt ggcgaagtga
    17401 ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc ggctgccagg
    17461 gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt ctcaccggtt
    17521 ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg gatgtgtctg
    17581 gtcgtcagca tactcacgac gcgccacgca cccgcacaca agacgcgatc gacgttaagt
    17641 tacgcgctta ataaaaagaa ttcagaatca ggccggagtg ctaaatactc cgtaaacacg
    17701 gtcgtacatc cagccggtag cctgattcct tgcattgagt gatgtacgga gtttatagag
    17761 caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac ccgcgtgttg
    17821 ccgagaaaat gatgcagttc atgacgatgg acggaacctt tggtaacccg gcctctcgtt
    17881 ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat cagattgccg
    17941 atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc gaatctgaca
    18001 acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac atcatcacca
    18061 gcaaaaccga acacaaagcg gtactggata cttgccgtca gctggagcgc gaaggtttcg
    18121 aagtcaccta cctggcaccg cagcgtaacg gtattatcga cctgaaagaa ctcgaagcgg
    18181 cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa atcggcgtgg
    18241 tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc tatcacgttg
    18301 atgcaaccca gagcgtgggc aaactgccaa tcgacctgag ccagttgaaa gttgacctga
    18361 tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg tatgtgcgtc
    18421 gtaaaccgcg tgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag cgcggtatgc
    18481 gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaagcctat cgcatcgcaa
    18541 aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt ctgtggaacg
    18601 gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt gcgccgaaca
    18661 ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg ctgaaagacc
    18721 tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc tacgtgctgc
    18781 gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct ttaggtcgtt
    18841 ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc atcggtcgtc
    18901 tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg aacagcatcg
    18961 aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct tacagcgaaa
    19021 aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgataac aacgacgaga
    19081 acgtcggcag cggcatggtg ggtgcaccgg cctgtggcga cgtaatgaag ttgcagatta
    19141 aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc tgcggttccg
    19201 ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc gacgaagcgc
    19261 aggcgatcaa aaacaccgat attgctgaag aacttgaact gccgccggtg aaaattcact
    19321 gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa agcaaacgtg
    19381 aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag tgcagcagcg
    19441 cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct gggcgtgaga
    19501 acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc gacgccggaa
    19561 gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag cctgcaattt
    19621 ctggacggta cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt taaattcacc
    19681 aacccgaacg ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg atgcgcatac
    19741 cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc gccgttatcg
    19801 cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc ttgcctgccc
    19861 gttatcaact cgatacccag gcactgagcc tgcgttttca ggatctacaa cgtcagtatc
    19921 atcctgataa attcgccagc ggaagcctgg cggaacaact cgccgccgta cagcaatctg
    19981 caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg gaatatttgc
    20041 tttctttaca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac accgcgttcc
    20101 tgatggaaca gttggagctg cgcgaagagc tggacgagat cgaacaggcg aaagatgaag
    20161 cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc catcagttga
    20221 tggttgaaca gttagacaac gaggcgtggg acgcggcggc ggatacggtg cgtaagctgc
    20281 gttttctcga taaactgcgg agcagtgccg aacaactcga agaaaaactg ctcgattttt
    20341 aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt gagtgctgcg
    20401 ccgcatcagc gtcgtctggc ggctggtatt gacctgggca caaccaactc gctggtggcg
    20461 acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca cctgctgcca
    20521 tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg tactaacgca
    20581 gcgcttgaca ccgccaacac cattagttct gttaaacgcc tgatggggcg ctcgctggct
    20641 gatatccagc aacgctatcc gcatctgcct tatcaattcc aggccagcga aaacggcctg
    20701 ccgatgattg aaacggcggc ggggctactg aacccggtgc gcgtttctgc ggacatcctc
    20761 aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg tgtagttatc
    20821 accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc ggcgcgtctg
    20881 gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat cgcctacggg
    20941 ctggattccg gtcaggaagg cgtgatcgct gtttatgacc tcggtggcgg gacgtttgat
    21001 atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg cggtgattcc
    21061 gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga gcaggcggac
    21121 attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc cattgcagcc
    21181 aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg gcagggcgaa
    21241 atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac cttactggct
    21301 tgtcgtcgcg cgctgaaaga cgcgggtgta gaagctgacg aagtgctgga agtggtgatg
    21361 gtgggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt tttcggtcgt
    21421 ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc gattcaggcg
    21481 gatattctgg tgggtaacaa gccagacagc gaaatgctgc tgcttgatgt gatcccactg
    21541 tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg taataccact
    21601 attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac ggcgatgtct
    21661 atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact ggcgcgtttt
    21721 gcgctgcgtg gtattccggc gctaccggct ggcggtgcgc atattcgcgt gacgttccag
    21781 gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg cgttgaggcg
    21841 tctattcagg tcaaaccgtc ttacggtctg accgatagcg aaatcgcttc gatgatcaaa
    21901 gactcaatga gctatgccga gcaggacgta aaagcccgta tgctggcaga acaaaaagta
    21961 gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc cgcgctgtta
    22021 agcgccgcag agcgtcaggt cattgacaat gctgccgctc acctgagtga agtggcgcag
    22081 ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca aacccaggat
    22141 ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca ttccgtggac
    22201 gaggtttaat atgccaaaga ttgttatttt gcctcatcag gacctctgcc ctgatggcgc
    22261 tgttctggaa gctaatagcg gtgaaaccat tctcgacgca gcgctgcgta acggtatcga
    22321 gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca tcgttcgtga
    22381 aggttttgac tcactgccgg aaagctcaga gcaggaagac gacatgctgg acaaagcctg
    22441 gggactggag ccggaaagcc gtttaagctg ccaggcgcgc gtcaccgacg aagatttagt
    22501 ggtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga ggttagtatg
    22561 ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc gtatcccgat
    22621 cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga tctggaagat
    22681 ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt gttagtctgg
    22741 ctggacgaag ccgagtgaat gaacgggctg cctgggggta gcccgttttg ctgttaaacg
    22801 aataaggata actaaaatga cagaagcgat gaagattacc ctctctaccc aacctgccga
    22861 cgcgcgctgg ggagaaaaag caacttacag cattaataat gacggcatta ccctgcattt
    22921 gaacggggca gacgatctgg ggctgatcca gcgtgcggcc cgcaagattg acggtctggg
    22981 catcaagcat gttcagttaa gcggtgaagg ctgggatgcg gatcgctgct gggcattctg
    23041 gcaaggttat aaaggcccga aaggcacgcg taaagtggag tggccggatc tggacgatgc
    23101 ccagcgccag gaactggata accgcctgat gatcatcgac tgggtgcgtg acaccatcaa
    23161 cgcaccggca gaagaattgg gaccatcgca actggcacag cgtgctgttg atctgatcag
    23221 caacgtcgcg ggcgatcgtg tgacttatcg gatcaccaaa ggcgaagatc tgcgtgagca
    23281 aggttatatg gggctgcaca ctgtcgggcg cggttcagaa cgttctccgg tattgctggc
    23341 gctggattac aacccaactg gcgataaaga agcaccggtg tacgcgtgcc tggtaggtaa
    23401 aggtatcact tttgactccg gcggctacag catcaaacag actgcgttta tggactcgat
    23461 gaagtcggac atgggcggcg cggcaacggt taccggggcg ctggcatttg ccattacgcg
    23521 cggactgaac aagcgcgtga agctgttcct ctgctgtgcg gataacctga ttagcggcaa
    23581 tgcgttcaag ctgggcgata tcatcaccta tcgcaacggt aaaaaagttg aagtgatgaa
    23641 cactgatgcc gaagggcgtc tggtgcttgc cgatggtctg attgatgcca gtgcgcagaa
    23701 accggaaatg atcattgatg cggcgaccct caccggggcg gcgaaaactg cgctgggtaa
    23761 tgattatcac gcgctgttca gttttgacga tgcgcttgcc ggtcgtttac tggcgagtgc
    23821 ctcacaagag aacgaaccat tctggcgtct gccgctggcg gaattccacc gcagccagct
    23881 gccgtctaac tttgccgaac tgaacaatac cggaagcgcg gcgtatccgg caggcgcgag
    23941 cacggcggcg ggcttcctgt cgcactttgt tgagaactat cagcaaggct ggctgcatat
    24001 cgactgctcg gcgacttacc gtaaagcgcc ggttgaacag tggtctgcgg gcgctacggg
    24061 acttggtgtg cgcacgatag ctaatctgtt aacggcgtaa actaatatgc cggatgcggc
    24121 gtaaacgcct tatccggcct acaaattctg caaattcaac aaattgcgaa tcccttgtag
    24181 gcctgataag cgtagcgcat caggcatttg cgccccgaac gttgaatgaa ttgtggttct
    24241 tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa aaagcagcaa ccgaaccggc
    24301 gcaccgcccg gcctttttcc gtactctact ggaatccacc gtctgggtgc ctggtacggc
    24361 ggcgcagggc gaggctgtgg ttgaagattg tgcgcttgat ttacagcact gggaaaaaga
    24421 agacggcacc agcgtcattc cttttttcac ctcgttagaa gcgcttcaac aggcggttga
    24481 agacgaacag gcatttgtcg taatgcccgt tcgcacattg tttgagatga cccttggcga
    24541 aacgctcttc cttaatgcca aactgccgac cggtaaagaa tttatgccgc gtgaaatcag
    24601 tttgttgatt ggtgaagagg gaaatccgct gagcagccag gaaatcctgg aaggcggtga
    24661 atcgctgata ttatcggaag tcgcagagcc gccagcacaa atgattgatt cactcaccac
    24721 cttatttaaa accattaagc cggtgaagcg tgcttttatt tgttcaatta aagagaacga
    24781 agaggcacag cctaatttac ttattggcat tgaagccgat ggtgatatcg aagaaattat
    24841 tcaggcgacg ggaagtgtag cgaccgatac attacctggc gatgaaccaa tcgatatttg
    24901 tcaggtgaaa aaaggggaaa aaggaattag ccactttatt accgaacata ttgcgccatt
    24961 ctatgaacgt cgctggggtg gttttttgcg tgactttaaa caaaaccgga taatctaaaa
    25021 tatgccgccc taaagggcgg cattcgggct tacttgctgc tctgaaggtt gttaaccacc
    25081 agtaaattgt cgccggagag actccggtta ttatttactt gggaaaacag cgcaccagaa
    25141 acaataagta cgatacagaa gcaccgtatc ttcattgtgc tgtcgtgatg cgaagaccgc
    25201 atcacgacag caccactggc gatattgccg cgatagcaag cgctgagacg cgcttgctct
    25261 aacggtcgcg gaacatcctt tccttaagcc agcgcccgct tgcgagacgg cggaaagggc
    25321 cgcagtggat aatacactaa cagaacaata ataactctct tttgcttgac aaaaagagag
    25381 ttactggcga gtattgtctc cgctgagacg cgcttgcatt actacggtaa tacaacctgc
    25441 tggaaaggcc gcgaaccgga ccagcaacaa ataaccgcct gattttggcg gttttttgtt
    25501 ttttaaagga aagtaaattt acatgtttaa gtaatatctt ttaccggtta gaatttttaa
    25561 ttttgagcag gatttgttta tatttgtctg aattcggact aataagccag gttattttat
    25621 taccgaacac ggcggaaagg gccggtgtcg ataatacgct aaataaacaa taattactct
    25681 cttttgcttg acaaaaagag agttactggt gagtattgtt ttactgtgaa gcacctgcgt
    25741 tgctcatgta acacaagtca acctgctgga aaggccgcga accagaccag caataaaaaa
    25801 acgccaaatt cggcggtttt ttattgcctg taaaatactt atttcactgg ctcaaccggt
    25861 aaatctgccc gcgcgcccca ttcactccat gcgccgtcgt acagtttcac gtttggcaca
    25921 tccagcgtcg cgagtgctaa caaaaccacg gctgccgtta caccagagcc gcagctgacg
    25981 ataatcggtt tgtcatagct gacgccgcga ccaaaaaata tcgcatccag ttcatcggtc
    26041 gtttttagtt cgccttcgcg caccagttcc gtccacggaa cgttcaatgc tccgggaata
    26101 tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg cgttaaaacg tgcagccggg
    26161 cgggcatcaa taatttgcgc cgtattttca tggcttgcca ataacacatc ggttactttc
    26221 accactgctt caggattaaa cgcggcgtta aactctcctt ccggcagctc tactgcacct
    26281 tcttctaata gcaggtcatc gcgctgccag cctgcaag
//
LOCUS       DADLTI010000043        25888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLTI010000043 DADLTI010000000
VERSION     DADLTI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.043"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            347..640
                     /locus_tag="JN755_001633"
     CDS             347..640
                     /locus_tag="JN755_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380468.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JN755_001634"
     CDS             712..1371
                     /locus_tag="JN755_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7380469.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JN755_001635"
     CDS             1448..1909
                     /locus_tag="JN755_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7380470.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JN755_001636"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JN755_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JN755_001637"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JN755_001637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7380471.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5074
                     /locus_tag="JN755_001638"
                     /pseudo
     CDS             3808..5074
                     /locus_tag="JN755_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JN755_001639"
     CDS             complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JN755_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7380472.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JN755_001640"
     CDS             complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JN755_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7380473.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7558..8277
                     /gene="fadR"
                     /locus_tag="JN755_001641"
     CDS             7558..8277
                     /gene="fadR"
                     /locus_tag="JN755_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7380474.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARTLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8329..9861)
                     /locus_tag="JN755_001642"
     CDS             complement(8329..9861)
                     /locus_tag="JN755_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7380475.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10191..11489
                     /locus_tag="JN755_001643"
     CDS             10191..11489
                     /locus_tag="JN755_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7380476.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11499..12569
                     /gene="dadX"
                     /locus_tag="JN755_001644"
     CDS             11499..12569
                     /gene="dadX"
                     /locus_tag="JN755_001644"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7380477.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12737..14163)
                     /locus_tag="JN755_001645"
                     /pseudo
     CDS             complement(12737..14163)
                     /locus_tag="JN755_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14773..16509)
                     /locus_tag="JN755_001646"
     CDS             complement(14773..16509)
                     /locus_tag="JN755_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7380478.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JN755_001647"
     CDS             complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JN755_001647"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7380479.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17618..18229
                     /gene="emtA"
                     /locus_tag="JN755_001648"
     CDS             17618..18229
                     /gene="emtA"
                     /locus_tag="JN755_001648"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7380480.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18231..18965)
                     /locus_tag="JN755_001649"
     CDS             complement(18231..18965)
                     /locus_tag="JN755_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7380481.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19166..19420
                     /locus_tag="JN755_001650"
     CDS             19166..19420
                     /locus_tag="JN755_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7380482.1"
                     /translation="MGIIARIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19470..21424)
                     /locus_tag="JN755_001651"
                     /pseudo
     CDS             complement(19470..21424)
                     /locus_tag="JN755_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21450..22304)
                     /locus_tag="JN755_001652"
     CDS             complement(21450..22304)
                     /locus_tag="JN755_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7380483.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22304..23113)
                     /locus_tag="JN755_001653"
     CDS             complement(22304..23113)
                     /locus_tag="JN755_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7380484.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23123..23881)
                     /locus_tag="JN755_001654"
     CDS             complement(23123..23881)
                     /locus_tag="JN755_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7380485.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23878..24858)
                     /locus_tag="JN755_001655"
     CDS             complement(23878..24858)
                     /locus_tag="JN755_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7380486.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24858..25880)
                     /locus_tag="JN755_001656"
     CDS             complement(24858..25880)
                     /locus_tag="JN755_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7380487.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6450 a         6374 c         6726 g         6338 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaacggttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta ccggtggttg tgctatcgaa
     3901 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggcg ttaaaatggg
     3961 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
     4021 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
     4081 gccccgcgtc gagatttaca gtattgatga agcattctgc gatctgacag gtgtgcgtaa
     4141 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     4201 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
     4261 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
     4321 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     4381 caaaaaactg gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg
     4441 gtttatccgt aaacatttta atgtcatgct cgaaagaacg gtgcgtgaac tgcgcggcga
     4501 accctgtttg caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc
     4561 gtttggtgaa cgcatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc
     4621 ccgggcggcg gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat
     4681 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcacgt cggtaaaact
     4741 gctgacgccc actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc
     4801 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     4861 tcagggagtc gcgcagctca atttattcga tgacaatgca ccgcgccccg ggagtgagca
     4921 attgatggcg gtaatggata ctctgaatgc taaagagggc agaggaacac tctattttgc
     4981 cgggcagggg atccaacagc aatggcagat gaagcgagct atgctttcac cacgttatac
     5041 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgttcc
     5101 cgcaggagcg ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct
     5161 gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     5221 gcggcatttc catacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa
     5281 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     5341 aggtggcaaa tggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     5401 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     5461 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac
     5521 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     5581 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     5641 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     5701 cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag
     5761 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     5821 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt
     5881 gtcagtacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     5941 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     6001 acagagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     6061 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     6121 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca
     6181 tgttcagatg cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc
     6241 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     6301 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgttgccag aataatgact
     6361 gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg
     6421 ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     6481 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     6541 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     6601 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     6661 atcaggttct gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg
     6721 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac gttgtaatgc
     6781 ttgtcgatat gactatcgtc ttgcaggtcg gtgtcttcgg tacgggaaga ggctacgcga
     6841 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     6901 tcgaggaacg cggagaggaa cgctgccgcc acgcaaaaag agagcgacag cagcattttg
     6961 gagcgaatgc ttaacagcaa acgggtaaat atgaacagca acagctgttt cataaaatag
     7021 atacccgcca ccataaacat caataacagc aagacttcaa gatttgccgc caccccttca
     7081 cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg
     7141 gggagtagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     7201 aaccagcccg cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg
     7261 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     7321 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttgtg
     7381 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     7441 agagatgcgg aaaatgaagc cttgatccct ttttcttctt tttgtctgct atcagcgtag
     7501 ttagccctct ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg
     7561 gtcattaagg cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg
     7621 aataaccgct tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt
     7681 ggcgtaacgc gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg
     7741 accattcaac atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat
     7801 atccttgaaa cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg
     7861 ctgtcggtgc gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc
     7921 gataaagcgc aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt
     7981 gccgagctgg attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac
     8041 ggtctgatcc ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc
     8101 aatccggaag cgcgcactct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt
     8161 gaaggcgcgc acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag
     8221 atttggcacc ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc
     8281 cttccgttta aagagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc
     8341 cattcttggc gggcaacgtt ccagcaactc gatgctgccg tcttcgtttt gctgttcgag
     8401 catcacatca aatccccaca ggcgatgcac atgcttcagg acttctttgc gcccccgatc
     8461 cagcggtgcg cgattatgtg gaatataacg cagcgtcagc gaacggtcgc cgcgcaaatc
     8521 cacgttccag atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg
     8581 gttacggatc tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg
     8641 ccgatcatca tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa
     8701 ctggctgata aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca
     8761 gtcggaaccg gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat
     8821 ccgtttaata tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca
     8881 cgggctgtta taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat
     8941 aaaacgttcc gttactttcc cttcatcata cagatggtta aggatggtat agtgccagaa
     9001 ggtcgcacag ccttcgttca taacctgagt ctgtttttgc ggataaaaat actggctcac
     9061 cttacgcaca atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc
     9121 cataaaatag agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt
     9181 tttctcttcc tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata
     9241 ctcttcgcga cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt
     9301 gtaccggtcc acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc
     9361 atcaacgcca taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc
     9421 gacaatcgaa ctggcgtcgg tccaactgcg gaataagcaa ttgtttttaa agaaagagtt
     9481 atgcccatag caggtgtgtg ccatcaccag cgcctgcata gtaatggtgt tctcttccat
     9541 caagtaagcg atacacgggt tagagttaat gacgatttca taggccagcc cttgctgacc
     9601 gtgcttatac agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt
     9661 aattggcatg ccgacgctgg aataggcatc catcatctgt tctgaggtta tcacttcaat
     9721 ctggtgcggg taagtatcca gccggtagag tttcgccacc cggtctatct ctgccagata
     9781 aacatccagc aggtcgaacg tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt
     9841 aatagaatcg atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc
     9901 acctcatttc aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt
     9961 gcgatttctt attatcagag tgccactaat ccgcttctga acggaatttt atgctggata
    10021 aaaagggcgt tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt
    10081 gagtcagctc accataaaaa agccgtatgt tgaataatat tttcaactga gttatcaaga
    10141 tgtaattaga ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg
    10201 tcatactggg aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac
    10261 atgaggtcac cgtcattgat cgggagccgg gggccgccct ggaaaccagt gccgcgaatg
    10321 ccgggcaaat ctcccccgga tatgctgcac cgtgggcagc accaggtgtg cctttaaaag
    10381 cgattaaatg gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt
    10441 ttcagttgaa atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa
    10501 acaaagggcg gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg
    10561 ccgaaaccaa tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg
    10621 aacaacagta tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt
    10681 atcagctgct ggaatccagc cgcctggcgg aagtggatcc cgcgctggca gaagtagcgc
    10741 acaaactgac gggcggcctg cagttaccca atgatgaaac cggagactgc cagctgttta
    10801 cccagaatct ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg
    10861 ttgaccaact gctttgcgac ggcgagcaaa tctacggcgt gaagtttggc gatgaagtga
    10921 ttaaggccga tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca
    10981 ttgttgatat tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg
    11041 aagatggtgc gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt
    11101 tcgataaccg cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt
    11161 tgcaaccgcg tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc
    11221 atgtcgagca ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg
    11281 ttgtcgggcg tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct
    11341 ggacgatggc ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag
    11401 cgatcccata tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc
    11461 cgggccattt acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca
    11521 gcctcgatct gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg
    11581 cgcgcgtctg gtcggtggta aaagcgaacg cttacgggca tggtattgag cgtatctgga
    11641 gcgcgctcgg ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac
    11701 gtgagcgcgg ctggaagggg ccgatcctga tgctggaagg atttttccat gctcaggatc
    11761 tggagattta tgaccagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag
    11821 cactgcaaaa tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga
    11881 tgaatcggtt gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa
    11941 tggcgaatgt tggcgaaatg accctgatgt cgcattttgc tgaagcggaa catcctgatg
    12001 gaatttccgg cgcgatggcg cgtattgagc aggcggcgga ggggctggag tgtcggcgtt
    12061 cgttgtccaa ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc
    12121 ctggcattat tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg
    12181 gattacgtcc ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg
    12241 gcgagcgtgt gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg
    12301 tcgccgcagg atacgccgac ggttatccgc gccatgcgcc taccggtact cctgttttag
    12361 tggacggcgt gcgcaccatg acggtgggga ccgtctcgat ggatatgctg gcggttgatt
    12421 taacgccttg cccgcaggcg gggattggta cgccggttga gctgtggggc aaggagatca
    12481 aaattgatga tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg
    12541 cgttacgcgt cccggttgtg acagtgtaac ttgttgtaag ccggatcgga ggcagcgtct
    12601 tctggttgcg aaaaaatcat ccatccggct ggtcagcaac tgtagttgtt aatgtgacag
    12661 agccattgcc catgatagtg tccattaaaa gaatggacac tatttccccg gaacctgaac
    12721 tcgccgcaca ggcgttctac ataaaacgct tacgcttcat tgttgactcg gagtcgactt
    12781 agataaatca cgcttcaccc ttgatgcaga ttcgggttca tgttcagatt gttgattaac
    12841 aggctgcgag tcctgttgtc tttccagact caacaggccc aactgttgat ccaggagctc
    12901 caggtcctcc tgtaccttca cgcgctccag gagttccggg tcatccagga actccagatc
    12961 cataaggtcc tccagctcct cgagtccttc caggtcaacc tagccttcct tgtgcaaaaa
    13021 gtccaaaagg tccttcaggc cctgctgttt tttcaggccc tcgacggctt tgagcagcgt
    13081 cggtaaattg tctttgtccc acgggttacc attacagtca aacaccttca ggctctgggg
    13141 ctgcggcacc tgtaccgttt taaacttgtt ttctccacag tagtaaatta ttagtttggg
    13201 cggcagccgt ggtgaagaga tcaaatcgtt actagaacag tcaaatactt tcaggcgagt
    13261 caagttctcc agcatctcat caggtattga gttaatattg ttgccggcac aggatagttt
    13321 atacaggtac tggggcatcg acggcggtaa aaattgcaag aagttttcag aacagttaat
    13381 cactctcagg ttagtcgaca gctcaggcag ccagataata cagttttcat agcacttcag
    13441 taccatcagt tttgagggct gcaccttcgg taacaagatc aagacgttat ctgtacagtc
    13501 aagcaccagc agccactttg gcatcacctt cggtaacgcg gtcaacacgt ttttactgca
    13561 gtaaaattca atcagatgtg gaggcaaatt ctcagggagt gaagtcaatc ccaggttgct
    13621 aagattgagc atactccgtc ttgtaatgag acattccttc attctgcgca atgcttctgc
    13681 ccggttttca atccggcttg ctggcaggtt ttcttccagg atattttcct tgatttcttc
    13741 cagactttct ttcaggtctt cttggggaat ttctgactgt ttgttttgct ggttttcttc
    13801 caggatttcg tacaggtttt tttgcaggtt ttcttgcaaa ccatcctgct gtatagccca
    13861 ttcgcaccat ttggcgacca tcatgaactc gttatgttgc tgattcaggc gagtccccaa
    13921 cgtgatgatg gtattgttgt ccacctgtat gttcactttg cccgcgatac tgaggtcaat
    13981 gatctgccag ggggcatcac cccagctgaa tttttgcggg ctgagatact tcatgttctg
    14041 caaaatttga ttcttgagtt ccaccgacag gaagagccgc cctggcgggc gtcgtacgtc
    14101 tgggttaacg gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc
    14161 cataatgcta cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg
    14221 tcaccgcaaa ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt
    14281 ttccctggtc agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct
    14341 ctgacgtatt aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag
    14401 gaataactat aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt
    14461 gtgggctatg tcagtgcggc aggttgcgtg gggaatatgt tcaggcgatg tcaggcaggg
    14521 gcccctgatg gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa
    14581 agcccttacg ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg
    14641 gtttgtcatc cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa
    14701 atgacaagtt tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat
    14761 gctgcattaa atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac
    14821 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
    14881 gacaaccggt gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc
    14941 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
    15001 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct
    15061 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc
    15121 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc
    15181 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc
    15241 agcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
    15301 gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga
    15361 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
    15421 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
    15481 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg
    15541 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
    15601 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
    15661 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
    15721 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    15781 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    15841 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    15901 aagaccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
    15961 gacactgctt tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat
    16021 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    16081 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    16141 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    16201 ggtgataagt acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga
    16261 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac
    16321 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag
    16381 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
    16441 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    16501 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    16561 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    16621 gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc
    16681 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    16741 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    16801 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    16861 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
    16921 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    16981 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
    17041 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    17101 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    17161 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
    17221 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    17281 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    17341 gggaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    17401 caataacctc gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    17461 gcgcggcgtg ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg
    17521 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    17581 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    17641 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
    17701 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    17761 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    17821 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    17881 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    17941 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    18001 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
    18061 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    18121 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    18181 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    18241 actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    18301 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    18361 tcattcttgc catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga
    18421 aaaacacccc attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat
    18481 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag
    18541 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
    18601 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
    18661 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    18721 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
    18781 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    18841 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
    18901 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    18961 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa
    19021 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc
    19081 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc
    19141 gattcatcga aacgagggcc tgaacatggg aattattgcc cggattattt ttggcctgat
    19201 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    19261 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    19321 tggcggctcc atcagcggtt ttaatttgca cagcttccta gtggcggtgg tgggcgctat
    19381 cctggtactg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    19441 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa
    19501 ttgcgacctt cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg
    19561 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    19621 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    19681 tccgacagcg ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc
    19741 tgcgttggaa gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga
    19801 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    19861 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc
    19921 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc
    19981 gtgaaggcac cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg
    20041 ttaacaatcg ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc
    20101 ggaaagcgtg tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat
    20161 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg
    20221 ccatctttct cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc
    20281 acgacatcga cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga
    20341 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    20401 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg
    20461 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    20521 agcgagttgt acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg
    20581 gtaaaacggt cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct
    20641 ggccactgcc agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt
    20701 tcaccatcct gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg
    20761 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    20821 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    20881 ctgacttgca aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    20941 ttgtcctgac tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga
    21001 tattaatggc tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa
    21061 cttcgactgc ccccagattg ttggtcagaa cccgcgccag atcgaggttg ccgtcatagg
    21121 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
    21181 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
    21241 ttaatgcctg agcgacattc tgtttatcca aggctttcat ggtgggttga tcgagaacgg
    21301 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg
    21361 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc
    21421 tcatagaata aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact
    21481 ttaatgtcag ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag
    21541 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga
    21601 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
    21661 agctgaagta cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct
    21721 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag
    21781 ccctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
    21841 ccggcacgat gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg
    21901 cccggaattg cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat
    21961 gccagcattt gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact
    22021 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc
    22081 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
    22141 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata
    22201 tagccaggct ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct
    22261 tccagcagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg
    22321 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
    22381 cttgttgttg ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
    22441 tccagctgac gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc
    22501 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
    22561 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
    22621 acgaggtgct gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc
    22681 tcattgcatg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
    22741 gtggcaggtc actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct
    22801 tcagtgcggc ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
    22861 aaaccgtagt gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat
    22921 cgaataaggt ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt
    22981 ccgttggcga ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg
    23041 ttttttctgt gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat
    23101 catcaatgag catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt
    23161 cggcagaaac ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc
    23221 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
    23281 ggagcggatg gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg
    23341 tgtccagaag ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca
    23401 acaagcgcgg ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
    23461 taagcgtatt ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg
    23521 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
    23581 ccatcaacac catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc
    23641 aggcaatagc ctgtgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
    23701 ggatctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
    23761 cgcaaccatt cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt
    23821 cgcgtagcac tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca
    23881 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
    23941 aatgccaagt gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag
    24001 cagaatagcg ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa
    24061 caagcgggca atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac
    24121 cgtgctggcg gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt
    24181 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
    24241 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
    24301 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
    24361 atcggccagc gttttaattg gcgatatccc cgctccgcac aacgttccta acgcaatgcc
    24421 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
    24481 gcccgcgacc accatcagct cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa
    24541 tccccacagg attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
    24601 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
    24661 tgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
    24721 ttcctgcaca gacacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
    24781 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
    24841 tccgtttacg attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa
    24901 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
    24961 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca
    25021 tcgagccagc caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc
    25081 acgccctgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
    25141 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga
    25201 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
    25261 ccctgtaagc ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg
    25321 gcgggtgagg tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt
    25381 tctgcggcgc ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
    25441 tgcgccagtt ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt
    25501 gcctgggaga tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
    25561 gccataagcc cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
    25621 aactggcgaa tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
    25681 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcgcctgcg
    25741 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact
    25801 gtttgtgaaa aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac
    25861 gtccgtctgg ttatgggcat ggtgaaca
//
LOCUS       DADLTI010000044        25448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLTI010000044 DADLTI010000000
VERSION     DADLTI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.044"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            65..523
                     /locus_tag="JN755_001657"
     CDS             65..523
                     /locus_tag="JN755_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7380488.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(533..1960)
                     /locus_tag="JN755_001658"
     CDS             complement(533..1960)
                     /locus_tag="JN755_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7380489.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1957..2664)
                     /locus_tag="JN755_001659"
     CDS             complement(1957..2664)
                     /locus_tag="JN755_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7380490.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2822..3805
                     /locus_tag="JN755_001660"
     CDS             2822..3805
                     /locus_tag="JN755_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7380491.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3875..4357)
                     /locus_tag="JN755_001661"
     CDS             complement(3875..4357)
                     /locus_tag="JN755_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7380492.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4592..7174
                     /gene="leuS"
                     /locus_tag="JN755_001662"
     CDS             4592..7174
                     /gene="leuS"
                     /locus_tag="JN755_001662"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7380493.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7189..7770
                     /gene="lptE"
                     /locus_tag="JN755_001663"
     CDS             7189..7770
                     /gene="lptE"
                     /locus_tag="JN755_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7380494.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7770..8801
                     /gene="holA"
                     /locus_tag="JN755_001664"
     CDS             7770..8801
                     /gene="holA"
                     /locus_tag="JN755_001664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7380495.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8803..9444
                     /gene="nadD"
                     /locus_tag="JN755_001665"
     CDS             8803..9444
                     /gene="nadD"
                     /locus_tag="JN755_001665"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7380496.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9468..10079
                     /locus_tag="JN755_001666"
     CDS             9468..10079
                     /locus_tag="JN755_001666"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY7380497.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10339..10656
                     /gene="rsfS"
                     /locus_tag="JN755_001667"
                     /gene_synonym="rsfA"
     CDS             10339..10656
                     /gene="rsfS"
                     /locus_tag="JN755_001667"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7380498.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10660..11127
                     /gene="rlmH"
                     /locus_tag="JN755_001668"
     CDS             10660..11127
                     /gene="rlmH"
                     /locus_tag="JN755_001668"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7380499.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11158..13059
                     /gene="mrdA"
                     /locus_tag="JN755_001669"
     CDS             11158..13059
                     /gene="mrdA"
                     /locus_tag="JN755_001669"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7380500.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13062..14174
                     /gene="mrdB"
                     /locus_tag="JN755_001670"
                     /gene_synonym="rodA"
     CDS             13062..14174
                     /gene="mrdB"
                     /locus_tag="JN755_001670"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7380501.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14185..15273
                     /gene="rlpA"
                     /locus_tag="JN755_001671"
     CDS             14185..15273
                     /gene="rlpA"
                     /locus_tag="JN755_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7380502.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15412..16623
                     /gene="dacA"
                     /locus_tag="JN755_001672"
     CDS             15412..16623
                     /gene="dacA"
                     /locus_tag="JN755_001672"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7380503.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16734..16997
                     /locus_tag="JN755_001673"
     CDS             16734..16997
                     /locus_tag="JN755_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7380504.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17098..17739
                     /gene="lipB"
                     /locus_tag="JN755_001674"
     CDS             17098..17739
                     /gene="lipB"
                     /locus_tag="JN755_001674"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7380505.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17998..18951
                     /locus_tag="JN755_001675"
     CDS             17998..18951
                     /locus_tag="JN755_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7380506.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19160..20125
                     /gene="lipA"
                     /locus_tag="JN755_001676"
     CDS             19160..20125
                     /gene="lipA"
                     /locus_tag="JN755_001676"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7380507.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JN755_001677"
     CDS             complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JN755_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7380508.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20558..21346)
                     /locus_tag="JN755_001678"
     CDS             complement(20558..21346)
                     /locus_tag="JN755_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7380509.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21439..21822
                     /gene="crcB"
                     /locus_tag="JN755_001679"
     CDS             21439..21822
                     /gene="crcB"
                     /locus_tag="JN755_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7380510.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JN755_001680"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JN755_001680"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7380511.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22260..22820)
                     /locus_tag="JN755_001681"
     CDS             complement(22260..22820)
                     /locus_tag="JN755_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7380512.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23409..24794
                     /gene="dcuC"
                     /locus_tag="JN755_001682"
     CDS             23409..24794
                     /gene="dcuC"
                     /locus_tag="JN755_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7380513.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24835..25176)
                     /locus_tag="JN755_001683"
                     /pseudo
     CDS             complement(24835..25176)
                     /locus_tag="JN755_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6515 a         6323 c         6250 g         6360 t
ORIGIN      
        1 ttttatagtt gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
       61 agccatggtg aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac
      121 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      181 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      241 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      301 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      361 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      421 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      481 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      541 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      601 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      661 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      721 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      781 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      841 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      901 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      961 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca
     1021 gatcacgcgc ttcaatcatc atcaccacta atgggtaata atcgacccga tgctgacggc
     1081 accattgcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa
     1141 gttctgccag caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
     1201 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
     1261 ggattcccag gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1321 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1381 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1441 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1501 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1561 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1621 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1681 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1741 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1801 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1861 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1921 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1981 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     2041 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2101 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2161 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2221 atgaatacgc gacaggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2281 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2341 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2401 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2461 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2521 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2581 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2641 tattttttgc gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgcaa
     2701 actacatttc gagactcagt catttatact tttctgacgc aacataatac gcataaaata
     2761 aggattacgc aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga
     2821 aatgataata atttttacgt caagttgctg cgataattta tcaatagatg agattatcga
     2881 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     2941 tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga
     3001 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
     3061 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
     3121 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga
     3181 agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3241 tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg
     3301 gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta
     3361 taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
     3421 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
     3481 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
     3541 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3601 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3661 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3721 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3781 tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc
     3841 catattctct ttattgctat gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3901 actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     3961 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4021 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4081 tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt
     4141 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc
     4201 gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4261 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg
     4321 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4381 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4441 aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4501 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4561 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4621 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4681 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4741 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg
     4801 caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4861 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4921 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     4981 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5041 taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5101 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5161 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5221 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5281 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5341 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5401 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5461 tgccgaagct gaaatggcga cgctggagaa aaaaggcgtc gatactggct ttaaagctgt
     5521 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5581 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5641 taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct
     5701 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct
     5761 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5821 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5881 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct
     5941 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6001 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6061 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6121 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6181 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6241 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6301 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6361 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6421 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6481 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6541 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6601 gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6661 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc
     6721 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6781 ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat
     6841 gcaggaagca ctgctggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt
     6901 cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     6961 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7021 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7081 ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt
     7141 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7201 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7261 gacaccacgc aggttccttc cactatgaag gtcatgatcc tagactcagg cgatccgaac
     7321 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7381 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7441 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7501 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt
     7561 tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7621 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7681 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7741 gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7801 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7861 tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc
     7921 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     7981 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8041 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8101 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8161 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg
     8221 ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct
     8281 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8341 tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8401 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8461 atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac
     8521 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg
     8581 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8641 atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8701 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8761 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8821 ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg
     8881 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     8941 ccccagccgg aagcaaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9001 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgca
     9061 caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt
     9121 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9181 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9241 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9301 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9361 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt
     9421 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9481 taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca
     9541 cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
     9601 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9661 ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg
     9721 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9781 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
     9841 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt
     9901 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg
     9961 ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10021 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10081 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    10141 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    10201 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10261 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10321 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10381 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10441 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10501 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10561 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10621 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10681 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10741 ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg
    10801 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10861 cgcattgtca tcctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10921 ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg
    10981 ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc
    11041 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11101 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11161 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11221 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11281 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11341 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc
    11401 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11461 ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa
    11521 gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta
    11581 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11641 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggata tgtgtcgaaa
    11701 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11761 acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11821 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11881 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11941 attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgatcc gcgtacaggt
    12001 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12061 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12121 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12181 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12241 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12301 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12361 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12421 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12481 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12541 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12601 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12661 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12721 gttgctaacc gccctaatgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12781 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12841 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12901 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12961 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    13021 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggct aatccgaata
    13081 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat
    13141 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13201 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13261 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13321 tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta
    13381 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13441 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13501 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13561 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13621 cagtagtgct ggtagcggcg ttcattccga ttctatggtt cttcctgatg catgattacc
    13681 agcgccagcg cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc
    13741 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13801 acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg
    13861 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    13921 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13981 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14041 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14101 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14161 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14221 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14281 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14341 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14401 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14461 aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg
    14521 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14581 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14641 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14701 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttat
    14761 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14821 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14881 ccggtaacca gtagcggttt cctcggcgct caaacgacct tagcgcctgg tgtactggaa
    14941 ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca
    15001 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    15061 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15121 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15181 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15241 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15301 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15361 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15421 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15481 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15541 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15601 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15661 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15721 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15781 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15841 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15901 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    15961 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16021 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16081 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16141 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16201 ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16261 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16321 ttcgcctcag aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa
    16381 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16441 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16501 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16561 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16621 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16681 actcccacct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16741 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16801 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16861 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16921 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    16981 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactctcctc
    17041 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17101 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg
    17161 gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc
    17221 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17281 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17341 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17401 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17461 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17521 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17581 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17641 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17701 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17761 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17821 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17881 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    17941 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18001 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18061 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18121 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18181 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18241 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18301 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18361 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18421 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    18481 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18541 catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct
    18601 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg
    18661 caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat
    18721 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18781 attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct
    18841 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18901 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    18961 gttacagcca gatcacagtg cagaacgata aaatggcaca atccaacaac aattttacat
    19021 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19081 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19141 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19201 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19261 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19321 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19381 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19441 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19501 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19561 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac
    19621 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg
    19681 tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa
    19741 cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc
    19801 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19861 gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19921 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    19981 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20041 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20101 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20161 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20221 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20281 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20341 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20401 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20461 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20521 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20581 agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20641 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20701 ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20761 aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc
    20821 cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg
    20881 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct
    20941 ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg
    21001 catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca
    21061 gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca
    21121 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21181 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa
    21241 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21301 tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc
    21361 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21421 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21481 ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21541 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21601 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21661 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21721 cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc
    21781 gcactggcat tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21841 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21901 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21961 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22021 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22081 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca
    22141 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22201 ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgcct
    22261 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22321 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22381 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22441 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22501 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22561 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22621 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22681 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct
    22741 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22801 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22861 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22921 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22981 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    23041 atgcaatcaa ggaaaaatta ttttattttt ttaacgagat acaacaatca tcttaacgaa
    23101 gtatataata tatatcgtca acccatgctc atattagatt attttttgag cgcacagccg
    23161 cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta
    23221 ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa
    23281 cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg
    23341 ggcatatttt ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag
    23401 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23461 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23521 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23581 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23641 acctcggcat gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg
    23701 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23761 tgctgatgat tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa
    23821 ccggtctggg tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca
    23881 gtcgtggcgc agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23941 caggggatgt ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca
    24001 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    24061 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24121 tcaccaccac tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24181 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24241 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24301 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24361 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24421 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24481 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24541 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24601 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    24661 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    24721 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    24781 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24841 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24901 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc
    24961 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    25021 gtttagcgtc aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata
    25081 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25141 tttacactgg cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga
    25201 aaaaaccggg agaacattgg attattcggg atctgatggg attagatttg gtggggcttg
    25261 caagcctgta gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt
    25321 gcttttgttt cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct
    25381 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtcacc aaatgactca tatcacaaat
    25441 cagctcat
//
LOCUS       DADLTI010000045        25429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLTI010000045 DADLTI010000000
VERSION     DADLTI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.045"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..105)
                     /locus_tag="JN755_001684"
     CDS             complement(<1..105)
                     /locus_tag="JN755_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY7380514.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQV"
     gene            complement(175..1164)
                     /locus_tag="JN755_001685"
     CDS             complement(175..1164)
                     /locus_tag="JN755_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7380515.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1961..2464
                     /locus_tag="JN755_001686"
     CDS             1961..2464
                     /locus_tag="JN755_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7380516.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2544..2795)
                     /locus_tag="JN755_001687"
     CDS             complement(2544..2795)
                     /locus_tag="JN755_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7380517.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JN755_001688"
     CDS             complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JN755_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7380518.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3259..3582
                     /locus_tag="JN755_001689"
     CDS             3259..3582
                     /locus_tag="JN755_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380519.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3753..4250
                     /gene="ftnA"
                     /locus_tag="JN755_001690"
     CDS             3753..4250
                     /gene="ftnA"
                     /locus_tag="JN755_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7380520.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4287..4526)
                     /locus_tag="JN755_001691"
     CDS             complement(4287..4526)
                     /locus_tag="JN755_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7380521.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4716..5927
                     /gene="tyrP"
                     /locus_tag="JN755_001692"
     CDS             4716..5927
                     /gene="tyrP"
                     /locus_tag="JN755_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7380522.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JN755_001693"
     CDS             complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JN755_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7380523.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6850..6936)
                     /locus_tag="JN755_001694"
     tRNA            complement(6850..6936)
                     /locus_tag="JN755_001694"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6900..6902),aa:Leu,seq:taa)
     gene            complement(6949..7022)
                     /locus_tag="JN755_001695"
     tRNA            complement(6949..7022)
                     /locus_tag="JN755_001695"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6988..6990),aa:Cys,seq:gca)
     gene            complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JN755_001696"
     CDS             complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JN755_001696"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7380524.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JN755_001697"
     CDS             complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JN755_001697"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7380525.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JN755_001698"
     CDS             complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JN755_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7380526.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10690..10866
                     /locus_tag="JN755_001699"
     CDS             10690..10866
                     /locus_tag="JN755_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380527.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10853..11077
                     /locus_tag="JN755_001700"
     CDS             10853..11077
                     /locus_tag="JN755_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7380528.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JN755_001701"
                     /pseudo
     CDS             complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JN755_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JN755_001702"
     CDS             complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JN755_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7380529.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JN755_001703"
     CDS             complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JN755_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7380530.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JN755_001704"
     CDS             complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JN755_001704"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7380531.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JN755_001705"
     CDS             complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JN755_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7380532.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JN755_001706"
     CDS             complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JN755_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7380533.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15968..16687)
                     /locus_tag="JN755_001707"
     CDS             complement(15968..16687)
                     /locus_tag="JN755_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7380534.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16852..18504)
                     /locus_tag="JN755_001708"
     CDS             complement(16852..18504)
                     /locus_tag="JN755_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY7380535.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18770..20176
                     /gene="fliD"
                     /locus_tag="JN755_001709"
     CDS             18770..20176
                     /gene="fliD"
                     /locus_tag="JN755_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7380536.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20201..20611
                     /gene="fliS"
                     /locus_tag="JN755_001710"
     CDS             20201..20611
                     /gene="fliS"
                     /locus_tag="JN755_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7380537.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20611..20976
                     /gene="fliT"
                     /locus_tag="JN755_001711"
     CDS             20611..20976
                     /gene="fliT"
                     /locus_tag="JN755_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7380538.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21054..22541
                     /gene="amyA"
                     /locus_tag="JN755_001712"
     CDS             21054..22541
                     /gene="amyA"
                     /locus_tag="JN755_001712"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7380539.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22575..22988)
                     /locus_tag="JN755_001713"
     CDS             complement(22575..22988)
                     /locus_tag="JN755_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7380540.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23175..24380
                     /gene="yedE"
                     /locus_tag="JN755_001714"
     CDS             23175..24380
                     /gene="yedE"
                     /locus_tag="JN755_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7380541.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24377..24610
                     /gene="yedF"
                     /locus_tag="JN755_001715"
     CDS             24377..24610
                     /gene="yedF"
                     /locus_tag="JN755_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7380542.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24717..25387
                     /locus_tag="JN755_001716"
                     /pseudo
     CDS             24717..25387
                     /locus_tag="JN755_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         6333 a         6487 c         6043 g         6566 t
ORIGIN      
        1 aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt
       61 tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat
      121 cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt
      181 accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac
      241 gtctgtaact tcggtaaatt ttggcgtttc aacgtctttt gctacccagt tgtaaagcat
      301 ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt
      361 tgcctgtgct ttagacagtt cgcttactgc atccacaccg ttaataccaa taccgatgat
      421 atcggctgct ttaaagccct gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc
      481 gttcataccg acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc
      541 agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc
      601 cgggaatcct gcagctttca gtgcgtccat agaacccgtt gtacggcggc gggcggtatc
      661 cagttcgttg gcggtaatcg ccatcactgc gctttctttg acatcccagc cacgtttctg
      721 catctcttta tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac
      781 cagaggaacg gtatccattg gcttaccttt ggcgttaaca aactggtcat ccactgcaat
      841 gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt tcgggtccgg
      901 agtacaaata acgaaacctt ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt
      961 tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt
     1021 atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc
     1081 gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc
     1141 cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc
     1201 aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag
     1261 ataagtgacc gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat
     1321 cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac ataaggaaaa
     1381 gccaatttgt cagacaaatt gttgaatgca cagcagatta atccataaga ttagcctgga
     1441 aatccttgtt gtcttcggta cccatgcggg atgtcttctt tttaaccagt caataggccg
     1501 cattacctgg cgttgagttt ttgaaatggt gtaataacca caactcaaag atgtggaaaa
     1561 tgcgcgtcat tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat
     1621 aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa
     1681 ttcaaattca tcgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc
     1741 atttacattg atttcaccaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt
     1801 tcttataacc gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat
     1861 cacatttcgg accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt
     1921 atcttgcacc tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct
     1981 tctcaaactc aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct
     2041 gagtaactgg tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc
     2101 acagagtaat gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac
     2161 ccccatcgtt aaagccattg atgttccagg tgaaaaactg aactctctgg aagaactgtt
     2221 ccaaaaaacg atggaagaat acgagcaacg ttcaagcacg ctggcgcagt tagccgatga
     2281 agcgaaagaa ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca
     2341 gcagcatgat ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc
     2401 gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca
     2461 ttgatcatac atcggcgcta atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc
     2521 atctgtttga agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca
     2581 atcagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc agcgggatga
     2641 tgagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg
     2701 attacatcga gaacatccag aatttgtttg ataaccaact ggcaagctac cacatcagaa
     2761 accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa
     2821 agccgttata gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca
     2881 caaaaaaacc tgccggagca ggttttttgt tatcggaaca tattgcctgg cggtacgtct
     2941 ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg
     3001 gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat
     3061 cacaaaaatc aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa
     3121 tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct
     3181 gccaggcatc ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct
     3241 tactcttctg gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg
     3301 gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta
     3361 cggttggttt atgaccaggc ttttttgcat catgcccata ccgatcgtta tgtcagtcgc
     3421 gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag
     3481 ccagtaggca actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg
     3541 tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg
     3601 ggatcttcgc tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat
     3661 aattgaagtt tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac
     3721 agcaacaaat ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac
     3781 ttaatgagca gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct
     3841 ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag
     3901 agatgacgca tatgcagcgt ctgtttgatt acctgactga taccggcaat ttaccgcgta
     3961 ttaataccgt tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa
     4021 cctataaaca cgaacaatta atcacccaga aaattaacga actggctcat gctgcaatga
     4081 ccaatcagga ctacccaaca tttaatttcc tgcagtggta tgtttctgag cagcatgaag
     4141 aagagaaact gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag
     4201 gtctgtattt tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg
     4261 cggcaggttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
     4321 aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
     4381 gctgtcatcc cttctgccga gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta
     4441 atcgcagctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
     4501 acttcatgac cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt
     4561 accgcttatg aagactttgc gccagcgcag gactgaatgc ttttattgta catttatatt
     4621 tacacaatat gtaacgtcga tttgacgaag cagccgttat gccttaacct gcgccgcaga
     4681 tatcactcat aaagatcgtc aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg
     4741 tttttatcgt ggcgggaacc acaattggcg caggcatgct ggcaatgccg ctggctgcgg
     4801 ccggtgttgg ttttagcgtt acgttaatct tgttgattgg gctttgggcg ttgatgtgct
     4861 acacggcgct attactgctg gaggtgtacc agcatgttcc ggcagatacc ggtctgggca
     4921 cgctggcaaa acgctatctg ggacgctacg gtcaatggct gacgggcttc agtatgatgt
     4981 tcttaatgta tgctctgact gcagcataca tcagcggtgc cggtgaattg ttggcctcca
     5041 gcatcagcga ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt
     5101 ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt cgatttattt aaccgttttc
     5161 tgttcagcgc caaaattatt tttctggtgg tgatgctggt attgctgcta ccgcatattc
     5221 acaaagtgaa tcttttaacc ctgccgttgc aacaggggct ggcactgtct gcaatcccgg
     5281 tgatttttac gtcgtttggt tttcacggta gcatgccgag tattgtcagc tatatggatg
     5341 gcaacattcg taagctacgc tgggtgttta taatcggtag tgccatcccc ctggtggcat
     5401 atattttctg gcaggtggcg acgcttggca gcattgattc aacaaccttt atgggattgc
     5461 tggctaatca tgctggatta aacgggctgt tacaggcgtt acgcgaaatg gtgccctctc
     5521 cgcatgttga gctggcggtg catttatttg ctgatttagc cctcgccacg tcatttctcg
     5581 gcgttgcgtt aggcttattt gattatctgg ctgatttgtt tcagcgttca aataccgttg
     5641 gtggacgatt gcaaactggt gcaattacct ttctgccgcc gttggcgttt gcactgtttt
     5701 atccacgagg atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg gtactggcat
     5761 tgattatccc ttcactattg acctggcaaa gcagaaagca caatcctcag gcgggttacc
     5821 gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg
     5881 gcgtgcaatt tttgattgcg gcagggttgt taccagaagt ggggtgatca gatagcctca
     5941 aattccttat tgggtgccag aattaacgct gacacccaat gtgacctctt aatgcaggca
     6001 gcactgctta aatttcttac cactgccgca cggacaagga tcgttacgcc ccggtttctc
     6061 ttctgctttg atcggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg
     6121 taaatcaagc gccgccagtc gaatggcatc tacgctctct tcaaacgctt ctggcgacat
     6181 tttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg
     6241 ttttaaagaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa
     6301 gcaccactcc tcaacaatcg tcagctcgct gccatcaact tctcgcaagc cgaataacgg
     6361 ctcaaactgc tccgggaatt cgttcagacg ctctgcggta tcggccatat gttgaaaagc
     6421 caggttcata aagcgcgtca tctctttctc tgacgcccaa cgcggcacat agtcagcccc
     6481 accccacacg gcaaccagcc actgttctgg ttcaatctct tgcggagaac tcaacaccgc
     6541 cgtcaataaa ccgtccagct ccgccacatc aaggatggcg tggtcagtgt tgtatttggt
     6601 cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt
     6661 ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac
     6721 tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc
     6781 acaaaaaaac cacccgaagg tggtttcacg acactgctta ttactttgat tttattctta
     6841 tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt
     6901 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     6961 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     7021 ccaaattctt caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga
     7081 gcgggaaacg agactcgaac tcgagacccc gaccttggca aggtcatgct ctaccaactg
     7141 agctattccc gtattcatca agcaatcagt taatcacttg attttattat cgtctggcaa
     7201 tcagtgccgc cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt
     7261 tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat
     7321 ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa
     7381 agaaaagtgc aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca
     7441 acgccaccat ctgggcagtg gttttcactt tcccaatcca ggagacggcc acgctactgc
     7501 gtttacccaa ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca
     7561 tcgttgccgc cggtaatgtc acccaccagc tgtgataatg ctcggttacc agcaccatgg
     7621 cgatagccac gagaacttta tctgccacag ggtcaaggaa agcaccaaac cgggtactct
     7681 ggttccagcg gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa
     7741 tgagcgcggc ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg
     7801 ggataaggat gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg
     7861 gtaactatct gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa
     7921 cgaccagaag atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc
     7981 gacgctggcg ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg
     8041 ttttggcccg acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt
     8101 tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg cgaatatgct ggataacatg
     8161 cagcgcgggt gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt
     8221 ttccagtcca gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt
     8281 atcccatgag acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc
     8341 gccgtcgata aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg
     8401 acgcagcacc tgattcatcg ccgcataatc atcgcccggc gtgatgcctg taatgttata
     8461 gcgccgatac tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt
     8521 ttgttcgccc atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt
     8581 caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct
     8641 ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg
     8701 aggtttggtt tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag
     8761 cgttttatcg ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct
     8821 gccttgtaaa tagaactgac ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc
     8881 aggcactttc gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac
     8941 gtggacacaa gccatgcccg catcgaacgc cacaccaata acgtcaaggt cgtcgccggt
     9001 attggaaaca aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca
     9061 tgccgcttct tcaaactcca gattctggct ggcagtttcc atacggctaa tgagttgcgt
     9121 aagcacctga tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta
     9181 ttcttcttca ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctggtattg
     9241 cagacacgga cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaaaat
     9301 cttttgcagt agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata
     9361 ttcacctttg gcatgctttg caccacgatg catcgccaga cgcgggtggg tatcgccact
     9421 caggaagata aaaggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata
     9481 gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt tctgtatgcg taaccgttac
     9541 atcaatttgc tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg
     9601 gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc
     9661 accagcatcg tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt
     9721 tgcgtcaaac tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga
     9781 attgccaggt gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag
     9841 cggtagctgt tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc
     9901 tggcccttag tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg
     9961 ctttctgttt tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca
    10021 atgtaacgct gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg
    10081 cctttgctga ggtagccagc agcacctgcc tgcatgactt tcgctggtaa agggttttct
    10141 gtatggaccg taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc
    10201 gcctcaagac cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt
    10261 gtccggcacc acttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc
    10321 tttatatctt ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt
    10381 agaacgttga tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat
    10441 tcgtatatta gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt
    10501 ttctctcgtt tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct
    10561 taccggatcg tttcacagta agccctacga tgaaatgtga caaaaattga ctttattcag
    10621 caaaaatgaa aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa
    10681 aaaacattca tgataaaaat atttattggt cattatatta acgtatttta tagcactgcc
    10741 gatatcacgc tcaaaaaaca accactgcta tttttagcaa agcttatggt atactccgcc
    10801 gccttaacat ttttcaccgc aaattttcat tgcaacatga cgaggaaaat aaatgagtac
    10861 gcctgatttt tctactgccg agaataatca agaactggca aatgaagtct cctgcctgaa
    10921 agcgatgctg acgctgatgc tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt
    10981 aaagatggaa aaacagcttg cgctgatcga agacgaaacc caggctgcag tattttccaa
    11041 aacggttaag caaattaaac aagcctaccg tcagtaatat aaaaccggct gagagcgtgc
    11101 ctttcagccg gttattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt
    11161 ccatttcaga atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc
    11221 atcatttaaa cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg
    11281 cagttcatca ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc
    11341 cagcgcccga tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg
    11401 cgcggcttcc cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggtc
    11461 ctgactaaag ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata
    11521 ataactaacc cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatag
    11581 taccgggtgg cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc
    11641 tatttcatgg tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg
    11701 acgccagctg aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta
    11761 tcatttataa tgataattca aactatcaca taaataagat ttatatataa tttatattat
    11821 tcaggcaatg aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc
    11881 tgatttgtta agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt
    11941 cggggctgat tttttcttat tattgcagca gaagcttctc gaggaactgg cgggtgcgag
    12001 gctgctgggg atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt
    12061 ccataaagat cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca
    12121 ccatcgtgcg cttttcctgc gccagctggc ggatggtgtt caggacttcg cccaccagct
    12181 ctggatccag cgccgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg
    12241 cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt
    12301 ctttaccggc cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt
    12361 tcggttcgcc tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg
    12421 gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg
    12481 atttttgctg acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc
    12541 ccgcttccgg ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac
    12601 tcggaccgat aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca
    12661 ccgtctgacc gtggaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc
    12721 tctctcctgg cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc
    12781 catgatccag tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga
    12841 ggtaatcaac tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt
    12901 atcttttacc agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg
    12961 tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc
    13021 ccactgacct ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc
    13081 agtgttcagc gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg
    13141 cggcaggccg taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga
    13201 gatataaaaa cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag
    13261 aataaacccg agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc
    13321 agcccctttc aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag
    13381 cgttcccgga gttaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga
    13441 ataaacagaa tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc
    13501 gccatcgctt ttccggtata cacaggatca agcagaatac cttccagacg cgccagcaat
    13561 ttcactgctt ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc
    13621 cagagtaaaa tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg
    13681 ttaaccactt tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg
    13741 ctttcaggca tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc
    13801 gatgcgacta ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc
    13861 gcactctcca cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac
    13921 gggcgaaagc cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg
    13981 gtcagtgcgt cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg
    14041 ttggttaaat agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc
    14101 agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg
    14161 gcagtaatca gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta
    14221 cgtaatttat tgccgcccat tgccatgggg gtgacctcat cccgtttgat gaaaatttcc
    14281 cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac
    14341 tccaaccgtg gaaaacgggt taaattatgc agtggcataa cagcctccga tgcgtgttgt
    14401 tgtgattttc ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg
    14461 cccttttttg tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa
    14521 gagcttgcag agtgccgtct ttttgcattt ccgcaattgc atcattcacc gctttcagca
    14581 ggtcttcatt tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg
    14641 ttactgccag cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga
    14701 ggatcgcatc gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg
    14761 tacgcacatc gacaccctga acattctgcc gcagccactc ttcatagttg gtgcccagac
    14821 cgacacccac ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt
    14881 ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct
    14941 tgcgctcgtc agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg
    15001 acgccagcat accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg
    15061 ccagctgttg agcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac
    15121 tgaacggcgg ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat
    15181 taagcagacc ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacgccca
    15241 tcacacccat caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg
    15301 ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc
    15361 ttattcttat ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg
    15421 tacaggtttg gcgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc
    15481 tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga
    15541 gggaaatatt ttgctcatgt agatgattac ccaaacggcg cagacgaacg acatattcac
    15601 gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa tttcttaaag ccgataatat
    15661 cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc
    15721 atgattgttg ttccgtttgc cagccttttt catcaagcag cgtgtccagg cgggaaatct
    15781 cgattttatt cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc
    15841 aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc
    15901 ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt
    15961 actgacgtta taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact
    16021 gactgacccg cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt
    16081 cctggtaata gagggttaat accagttttt cgcgctccgg caacgtttcg atggcttcca
    16141 tcacccgctg gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat
    16201 catcagtaac cagttcgatg ctatcgccgt gctcttcacg ccactcatcg taggagaaga
    16261 gctggctgtt attggtgtcg agcagcattt gacgataatc ggcaatatcg atccctaaac
    16321 gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg
    16381 cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa
    16441 gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt
    16501 gtagggcgtc atagcgttcg acagcattaa gtaacccaat gccgcccgcc tgtagcagat
    16561 cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacacacca
    16621 gcgggacata acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg
    16681 aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag
    16741 ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag
    16801 ccgaatgtaa aaaaccccgt cggtggcggg gtttgagagg cattcagacg attaaccctg
    16861 cagcagagac agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc
    16921 ctgcaggata atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
    16981 aatacgggac tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga
    17041 atccaaacgg ttttgcaccg cacccaggga ggagcggaat ttgtcgatgg agctgatggc
    17101 ttcatccaga gctttcaggg gatcggctgt cgtagctgct tttgtttcag catctgtcgt
    17161 caatttacca tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc
    17221 atcctgtagg taaaaatcta cagtatcaga tgtggtcaac gcctcgttgt tatcaacata
    17281 aaccgaatca acaccttttg ttacagcgcc agttgtgccg ttgacgctat aacctacagt
    17341 agtgaaatca acattgtttc ccgaggttga tacagcttta tcgaaagctg cctgatcaac
    17401 ttttgcacct gttactgaat aagtaatagt gtccggcgta gtactattac cggtaaagga
    17461 gatagaggta ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga
    17521 cagactagcc tcagtcaaag aagcaccaga gccgtttttc gttaagcgcc cagttgtatc
    17581 aatgtagagt ttatctccat tgcttgccgt aattttacca tcactgctga tctgaacatc
    17641 ctgtgcagaa ccatcgatag taatagtgcc cttagcggta tcaccaactc ccggggtcaa
    17701 atatttctga acatcggcag ctgaagccgt tgtggtatca aaagagtatg acttacttgc
    17761 actgtcatac ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc
    17821 ctgaacagta tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta
    17881 acccgcactc acagtatatt tgttgccgac cactgttgca ttagcagcta ccaagtcagc
    17941 tttagatgca gcagtattag ccacagcccc gccaccattc acattaaacc cattcagccc
    18001 cagcgtatca gagtcaattt tcttcagatc aatagtgata gtctggccgt cattcgcacc
    18061 aacctgaatt ttcatcgaac cgtcttttgc cagtacgttc acgccgttga actgggtctg
    18121 accggatacg cggtcaattt cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag
    18181 atccgaatca gagttagtcc cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa
    18241 gttgttgttg atttcggaca gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt
    18301 ggcgttacgt gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc
    18361 ctgacccgcg gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat
    18421 agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct
    18481 gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt
    18541 tttgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca
    18601 acctttagaa tttttttcac aaacagccat tttttgttag tcgacgaaat actcttttct
    18661 ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc
    18721 cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat
    18781 ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc
    18841 cgcgcaaaaa gcgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact
    18901 tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt
    18961 gtctaaagcc gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc
    19021 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    19081 ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag
    19141 cgacagcaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    19201 cgcggctaac tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt
    19261 aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga
    19321 caccggcctt gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt
    19381 tatgggctat gacgccagtg ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa
    19441 tgcgcagctg acggtcaaca acgtcgccat cgagaacagc agcaacacta tcagcgacgc
    19501 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    19561 cactcaggac acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc
    19621 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    19681 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    19741 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat
    19801 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    19861 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    19921 cggcatcaca accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    19981 taaagccgct accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc
    20041 cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    20101 cgttttaatg acctcgttaa acagcaccag cagctactta acgcagcagt tcgaaaacaa
    20161 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    20221 ccaggcctat gcgcaaattg gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt
    20281 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgac tgtttatgca
    20341 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    20401 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttaattgc
    20461 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    20521 agtcgaagag gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact
    20581 ctccccttct tcgattcagg acccagtctg atgaacaatg caccgcattt gtatttcgcc
    20641 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    20701 gacgaactca tcgctagcga aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg
    20761 actgaagagg ttgccccgtc cactacgatg caggagcagc tccgcccgat gctgcacctg
    20821 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    20881 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc
    20941 ggctttgtgc tggctccgca ggataacctc ttttgaatct taatgagccg atggctcgcg
    21001 aataatccga ttacggctac gcttctaata ttccccttga atggagtcga agaatgcgta
    21061 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    21121 aactggccga gcgcgcagac ggttttaatg atattggtat caatatggtc tggttgccgc
    21181 ccgcctataa aggcgcatcg ggcggctatt cggtcggcta cgactcctat gatttatttg
    21241 atctaggtga gtttgatcag aaaggcagca tccctactaa atatggtgat aaagcacaac
    21301 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgctg gacgtggtag
    21361 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg
    21421 atgaccgtac gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct
    21481 tccccgctcg tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta
    21541 tcgaccatat cgaacaccct gatgaagatt gcatttttaa aattgttaac gactataccg
    21601 gagaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg
    21661 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    21721 tgatggaaca aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat attccggcat
    21781 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    21841 tggctgaata ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag
    21901 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    21961 gtcgcgacta cgacatgacg cagatcttca ctgggacgct ggtggaagcc gatccttttc
    22021 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    22081 tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc
    22141 cttcggtgtt ctatccggac ctctacggtg cacattacga agatgtcggt ggtgacgggc
    22201 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    22261 agcgtttcgc ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta
    22321 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    22381 gcgaaaaaac cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg
    22441 ggaaccggca agagagcgta gtgacagacg aaaacggcga agcgaccttc ttttgcaacg
    22501 gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aatccatccc cggcggcaag
    22561 ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca
    22621 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    22681 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    22741 atcaatgtca acttacccgg cacggcaatc ccccgctgcc actgacggca atccaaggta
    22801 tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc
    22861 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    22921 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    22981 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gctctgatgt
    23041 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
    23101 ccccgaaaat aaatttgcat ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt
    23161 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
    23221 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
    23281 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
    23341 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
    23401 taacccgcat cgacggcatg atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc
    23461 tgtgggccaa caatgtcaaa ctacgcatgc cacgcagccg tatccgcatt atgcaggcca
    23521 tcattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
    23581 cgttctttac cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
    23641 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
    23701 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
    23761 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga
    23821 tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
    23881 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
    23941 atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg
    24001 tacagttagg cgttgaacct aaaatcatgt gggcgggacc aaatgcggta attggtggtt
    24061 tactttttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
    24121 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga
    24181 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
    24241 acctgctgaa aacttttggt ccgatgggag gcctgctggt gacatatttg ctgctgtttg
    24301 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
    24361 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
    24421 accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga
    24481 gatcctagaa gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg
    24541 taatcacggc tacactgtgc tggatattca gcaagacggg ccgaccattc gttatttaat
    24601 tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aacatcgtta cctctgacac
    24661 atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag gttgctatgt
    24721 gtggacgctg tgcccaatcc caaacgcgtg aggattacct tgcgcttctc gcggaagata
    24781 ttgaacgcga tattccctac gatcccgaac ccattggcag atacaatgtc gcgccgggaa
    24841 ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg
    24901 gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcct
    24961 caaccagtcg catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg
    25021 gctggtttga gtggaaaaaa gaaggcgaca aaaaaagcct tattttatct atcgcgctga
    25081 cggacaacct gtttttatgg ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc
    25141 cgaaggattt ttgatcgtca ctgcggcggc agatcaaggt ctggtagata ttcatgaccg
    25201 ccgcccgctg gttctgtcgc cagaagctgc acgggaatgg atgcggcaag acattggtgg
    25261 taaagaagcc tcagaaatcg ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca
    25321 ccctgtgtcg cgcacggtgg gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt
    25381 ttgttgacat cctccacgcc ctgaatgacg aggccccctg ctacgttca
//
LOCUS       DADLTI010000046        24860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLTI010000046 DADLTI010000000
VERSION     DADLTI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.046"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            72..353
                     /locus_tag="JN755_001717"
     CDS             72..353
                     /locus_tag="JN755_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7380543.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="JN755_001718"
                     /pseudo
     CDS             319..1396
                     /locus_tag="JN755_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1789..2130)
                     /locus_tag="JN755_001719"
     CDS             complement(1789..2130)
                     /locus_tag="JN755_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7380544.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JN755_001720"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JN755_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JN755_001721"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JN755_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7380545.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /locus_tag="JN755_001722"
     CDS             3532..3762
                     /locus_tag="JN755_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7380546.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="JN755_001723"
     CDS             3864..4520
                     /locus_tag="JN755_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7380547.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="JN755_001724"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="JN755_001724"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7380548.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JN755_001725"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JN755_001725"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY7380549.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="JN755_001726"
     CDS             complement(7472..8131)
                     /locus_tag="JN755_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7380550.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN755_001727"
     CDS             complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN755_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7380551.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN755_001728"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN755_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="JN755_001729"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="JN755_001729"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7380552.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /locus_tag="JN755_001730"
     CDS             complement(10538..11179)
                     /locus_tag="JN755_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7380553.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JN755_001731"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JN755_001731"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7380554.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JN755_001732"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JN755_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7380555.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /locus_tag="JN755_001733"
     CDS             15075..15944
                     /locus_tag="JN755_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7380556.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="JN755_001734"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="JN755_001734"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7380557.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JN755_001735"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JN755_001735"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7380558.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JN755_001736"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JN755_001736"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7380559.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JN755_001737"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JN755_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7380560.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="JN755_001738"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="JN755_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7380561.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="JN755_001739"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="JN755_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7380562.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JN755_001740"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JN755_001740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7380563.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JN755_001741"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JN755_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7380564.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /locus_tag="JN755_001742"
     CDS             complement(24538..24603)
                     /locus_tag="JN755_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380565.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24860
                     /locus_tag="JN755_001743"
     CDS             24674..>24860
                     /locus_tag="JN755_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380566.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTIT"
BASE COUNT         6169 a         6294 c         6105 g         6292 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc
     1141 cgggattaga aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt
     1201 tgtcgcagcc agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccctag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgacctg gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgc taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgtgatttt atacagcata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctact tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca
//
LOCUS       DADLTI010000047        24799 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLTI010000047 DADLTI010000000
VERSION     DADLTI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.047"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JN755_001744"
     CDS             complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JN755_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7380567.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1078..4128
                     /gene="fdnG"
                     /locus_tag="JN755_001745"
     CDS             1078..4128
                     /gene="fdnG"
                     /locus_tag="JN755_001745"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1663..1665,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7380568.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4141..5043
                     /gene="fdxH"
                     /locus_tag="JN755_001746"
     CDS             4141..5043
                     /gene="fdxH"
                     /locus_tag="JN755_001746"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7380569.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5040..5675
                     /gene="fdoI"
                     /locus_tag="JN755_001747"
     CDS             5040..5675
                     /gene="fdoI"
                     /locus_tag="JN755_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7380570.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5672..6601
                     /gene="fdhE"
                     /locus_tag="JN755_001748"
     CDS             5672..6601
                     /gene="fdhE"
                     /locus_tag="JN755_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7380571.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6931..7173)
                     /locus_tag="JN755_001749"
     CDS             complement(6931..7173)
                     /locus_tag="JN755_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380572.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7391..7609)
                     /locus_tag="JN755_001750"
     CDS             complement(7391..7609)
                     /locus_tag="JN755_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7380573.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8288..9433)
                     /locus_tag="JN755_001751"
     CDS             complement(8288..9433)
                     /locus_tag="JN755_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380574.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9591..9929)
                     /locus_tag="JN755_001752"
     CDS             complement(9591..9929)
                     /locus_tag="JN755_001752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380575.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JN755_001753"
                     /gene_synonym="yiiD"
     CDS             complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JN755_001753"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7380576.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JN755_001754"
     CDS             complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JN755_001754"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7380577.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11939..12811)
                     /locus_tag="JN755_001755"
     CDS             complement(11939..12811)
                     /locus_tag="JN755_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7380578.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JN755_001756"
     CDS             complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JN755_001756"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7380579.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13503..14288)
                     /locus_tag="JN755_001757"
     CDS             complement(13503..14288)
                     /locus_tag="JN755_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7380580.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(14322..15218)
                     /gene="yihV"
                     /locus_tag="JN755_001758"
     CDS             complement(14322..15218)
                     /gene="yihV"
                     /locus_tag="JN755_001758"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7380581.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15386..17041
                     /gene="yihU"
                     /locus_tag="JN755_001759"
     CDS             15386..17041
                     /gene="yihU"
                     /locus_tag="JN755_001759"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7380582.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARASGGFA
                     MLAVDQREAMRMMFAAAGAPAPVADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVV
                     EQNAIAKSCAMIVAADEFIPGNGIPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDE
                     DAQQRLDMVKEFNGLCHSHGLVSIIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADL
                     YKVEMPLYGKGPQQELLCASQRLNDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGF
                     LAGRAVWASVVGLPDNELMLRDVCAPKLQQLGDIVDEMMAKRR"
     gene            17058..18299
                     /gene="yihS"
                     /locus_tag="JN755_001760"
     CDS             17058..18299
                     /gene="yihS"
                     /locus_tag="JN755_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7380583.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18413..19339
                     /locus_tag="JN755_001761"
     CDS             18413..19339
                     /locus_tag="JN755_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY7380584.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19538..21574
                     /gene="yihQ"
                     /locus_tag="JN755_001762"
                     /pseudo
     CDS             19538..21574
                     /gene="yihQ"
                     /locus_tag="JN755_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21620..23005
                     /locus_tag="JN755_001763"
     CDS             21620..23005
                     /locus_tag="JN755_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380585.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23048..24451
                     /locus_tag="JN755_001764"
     CDS             23048..24451
                     /locus_tag="JN755_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380586.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24519..>24799
                     /locus_tag="JN755_001765"
     CDS             24519..>24799
                     /locus_tag="JN755_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380587.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         6137 a         6380 c         6092 g         6190 t
ORIGIN      
        1 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
       61 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
      121 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
      181 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
      241 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
      301 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
      361 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
      421 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
      481 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
      541 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
      601 tcaagaccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
      661 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
      721 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
      781 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
      841 atatttgtca cattttcgat ttcttttcgc tgtgtttttt tcactttctc ttaaccagat
      901 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctaga tggagggaaa
      961 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
     1021 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
     1081 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
     1141 gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa cccggcagta taaactgctg
     1201 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
     1261 ttcagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
     1321 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
     1381 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
     1441 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
     1501 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
     1561 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
     1621 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
     1681 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
     1741 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
     1801 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
     1861 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
     1921 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccacgaatac
     1981 accgaagcct ataccaacgc cagcctgatc gtgcgtgagg attacggctt tgaagatggc
     2041 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
     2101 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
     2161 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
     2221 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
     2281 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
     2341 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
     2401 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctactgtcg
     2461 cagagtctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
     2521 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
     2581 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
     2641 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
     2701 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
     2761 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
     2821 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
     2881 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
     2941 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
     3001 gccccgggga ttacgctgac tgacggtgag atcctctccg gtatcttcct gcgcttgcgc
     3061 aagatgtatg ccgaacaggg tggcgcgaac ccggatcagg tgctgaacat gacctggaac
     3121 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
     3181 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
     3241 agctcgttcg cccaattgcg tgatgacggt acaacctcct gtggctgctg gattttcgcc
     3301 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
     3361 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
     3421 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
     3481 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
     3541 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
     3601 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
     3661 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
     3721 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
     3781 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcagccaga gcagtttgtg
     3841 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
     3901 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgcacgctg
     3961 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ctatgaaggc
     4021 gtagctaaaa aaggctttat tgccaatact ttaacgccat tcgttggtga tgcgaacacg
     4081 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
     4141 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
     4201 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgttac cacctgtatc
     4261 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
     4321 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
     4381 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
     4441 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
     4501 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
     4561 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
     4621 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
     4681 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
     4741 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
     4801 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
     4861 ccggagaacc cggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
     4921 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
     4981 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
     5041 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
     5101 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
     5161 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
     5221 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat caaaacctaa
     5281 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
     5341 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
     5401 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
     5461 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
     5521 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
     5581 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
     5641 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
     5701 gctgggttcg agcgagaaac gtacggcgaa tatgattccg ccgttattgt tccctcggct
     5761 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
     5821 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
     5881 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
     5941 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
     6001 aattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
     6061 gaaggcatcg actcaggagc tggaagatat ggccagcgcg ctgtttgcct ctgatttctc
     6121 gtccgtcagc agcgataaag cgccgtttat ctgggctgcg ctgtcgctct actgggcgca
     6181 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg
     6241 cccggtatgt ggctctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
     6301 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
     6361 caactgcgag cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
     6421 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
     6481 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
     6541 gcaagaaggc tacgcccgta gttccattaa cccgttcctg tttccgggtg aaggagagta
     6601 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagctcatag ggtgtgacga
     6661 caccatctct cgtattccgc gttacccatt caagatcatg ccgcaattgt ctgtttgcct
     6721 cgttcttccc tccaacacat cgataaggtt atgggtatgt cactgatgac aagcgtatga
     6781 ttgcctgatg cgctgcgctt atcaggccta ccaataaact tcaatatatt gaatttgcat
     6841 gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg cgcacgttgt
     6901 cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct tacgctgata
     6961 ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc ttgctgtttg
     7021 cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac gaggaagatt
     7081 acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat cgagtaaaat
     7141 tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat catttttgaa
     7201 tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta cgcatcattt
     7261 tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt ataagttcaa
     7321 gtaaaacagc cttgtattaa taccagaata agggcatctg ttttttatat tcaagaatga
     7381 aaaatttttt tcattcctta tgctccttac actgatcatc aacaccaact acctccccct
     7441 tttgcctgcc tccctttgct ttacgcaccg gaaattgctg ccgctccaga tattgctcaa
     7501 ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca gccccgaagc ttgttcagcc
     7561 gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaccacct
     7621 tacatatatt actcattaat gtatgtgcga aaattacgcc gaaaaagcat ttaaaaaaat
     7681 gcggattttt gcatgtcatt ttgtttttgc gaggcgtttt ccagaaataa taagagcgtt
     7741 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt
     7801 gctatgaata tattatattc acggatattt atttgcataa tctgaatggt gtagccgaaa
     7861 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
     7921 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
     7981 tcgggaaaca aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca
     8041 atcatcagcg cgacgatgag tttttttaat cgcatcatag ggtggcgacg ttcagcacga
     8101 ttacgggtca tagcaaaact cctgagcagg cggccactat gccgctttca ctggagttct
     8161 gaatactggt actttctgta tggcttcgcc atgcccctcg cggctaagta cctgcacaca
     8221 tctctctttg tatgtcattt ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt
     8281 ttactcgtca gactcccatc cctacggtat acgagttata ttcatgaatg aaatcgacat
     8341 ttttaaaaaa cgaatcttgt aaaacatcag tgttgatatt accttgcaat ccaacattga
     8401 atttgttaga gacatgaaga actcgaatat caaaaaggtt ttttaacatc attaattctt
     8461 cgggtgttaa tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttctc
     8521 cactatcggc gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg
     8581 atatcgtgcg atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct
     8641 caagagtttc atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat
     8701 ttctttcgtg atttaattta tctaccagtt cattccattt atcaaaagcc gggctatatt
     8761 tgttgcgcac agaaatatcc gactggttaa acaattcagc catgcgattt tttatcatct
     8821 cgatatccgg cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca
     8881 ttaatgccaa gcccacaact gtcgccatga tgggcgcagc cacggttttg gcaagggcga
     8941 cgctgcgatg gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt
     9001 ccttacatgc ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca
     9061 aaaaaacttt ctgcttaatt gcactaagaa aacgcaaatc aaaaataatt gtttttggtt
     9121 tattattttt tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat
     9181 ttttttgcac agcacataac acaattgatg aagccttctc cactgcatta ttgattttaa
     9241 ttttaaaatc cgcatgatat acgttgacgg gcaatgcaag tatatctgga cctacatctt
     9301 ttgctgagta tttattggtg atcgacggac gtgaacccac gagaaaactt agctgcaaat
     9361 tatgctctat attatcagct gtttttgtgg tgtggttaaa catagagatg ccacctttca
     9421 ttctttcaca catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacat
     9481 taatttgatg atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc
     9541 catacaggct aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatacttg
     9601 aagcattcac aaagagggtc aatattataa ctgtttataa cttcacctac atcaagtgtg
     9661 tagtgcttac tctggagact gccatcctta tttaattgta tatcaatcaa tatatccccg
     9721 gactttgtca ccatatcttt gccataaacc atttgcatag ccatttcgag gagattttta
     9781 tctaactgat tgcgggcgtt gagtaagcga ttaccaaata atttatatac tgttcccgtc
     9841 attgccagcg ctgcgctttc tggtactttg cgtttccaca gtgaggtttc tctattttta
     9901 aacaggtcat attcattatt actcgacaaa ccatgcttca gttcgttaac acaatcgcac
     9961 agttgctcct caccttccat tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc
    10021 ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta
    10081 atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca
    10141 acataatgat tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc
    10201 cattatcttt gcataatgtt tgtgagctat agattttaaa aatatttatg aaaaaagtat
    10261 ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt
    10321 aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac
    10381 agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt
    10441 caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc
    10501 accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
    10561 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
    10621 gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc
    10681 gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg
    10741 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca
    10801 gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg
    10861 gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
    10921 ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag
    10981 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat
    11041 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc
    11101 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
    11161 ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg
    11221 cacgtccggg tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa
    11281 tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
    11341 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
    11401 gcgaaactga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat
    11461 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
    11521 aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt
    11581 tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg
    11641 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg
    11701 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
    11761 ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct
    11821 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg
    11881 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
    11941 atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag
    12001 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
    12061 gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat
    12121 aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa
    12181 tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga
    12241 tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc
    12301 tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg
    12361 gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt
    12421 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga
    12481 cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga
    12541 tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta
    12601 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
    12661 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
    12721 tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt
    12781 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
    12841 ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc
    12901 ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg
    12961 ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc
    13021 agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt
    13081 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
    13141 ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
    13201 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
    13261 ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat
    13321 acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt
    13381 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
    13441 gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa
    13501 atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt
    13561 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
    13621 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg
    13681 tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca
    13741 tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag
    13801 gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc
    13861 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
    13921 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg
    13981 gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt
    14041 tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac gctggaagcc
    14101 cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct cgcttaattt acgaatatcc
    14161 cggcggaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat attcatatac
    14221 ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt tagttcggta
    14281 aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa aagtgacaaa
    14341 aaagatcggg tttgatcaca gtcagggatc ccggctcgtc caccgggacg tgtgcatttt
    14401 aacgccgcta caccgctggc gaagcggact gactccgcta aatccccact tgttgccagc
    14461 gccaccgcca aagcgccgtg aaaaacatca cccgcacctg tggtatctac cacatcaact
    14521 ttgaaggccg gttgatgctg acgcccacca ttttccagcc agtcgcagcc tgcgctaccc
    14581 tgggtcacat agacatgacc atttgtgagc gtttgtgcct gttttagcgc actggccatc
    14641 tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat
    14701 gccaccagct cactgatatc ctgcggcgta atgtccccgt ccagaacggt catcacaccc
    14761 gcctgacggg ccagggtgaa ggctttttta acgccgtcgt gccagcgtac atctgccagc
    14821 acaacatccc actgagagaa atcaatttcc tccaaccact ctgcgtcagg cagcagatcc
    14881 gggctggggt agttaatgat tatccgctcg cctttggtat ccaccatgat ggcggattgc
    14941 gaagatttcg cctggttata ccgtttggtg taacgggtgt taacgcccca ggattccagc
    15001 tctgctagca ggctgttgcc cgcgtcgtca tcacctacgc gaccaataaa atcgacctgc
    15061 gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc aacttccgta
    15121 taatttctcg ccacgtattt accgctctcc gtcggtaacc cttccacgta atagatgcga
    15181 tccatcacgg ttatacctac acaagcaaca cgaatcatgg tcattcctta aacattttgg
    15241 gatgcgttca ttttcatttc accaattgtt taaaaagtga cggctgtcaa ttttttgacg
    15301 ataaattaca aatgcgatca aaaacagaca agcaaagcat gtcataaatg acaaaaagtg
    15361 acatcactgt attcaggaga gggttatggc agcaatcgcg tttatcggtt taggacaaat
    15421 gggttcgcca atggcgagca atttattgca gcaagggcat caacttcgcg tctttgatgt
    15481 gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg actcccgccg ccaacccggt
    15541 gcaggccgct aaagatgccg aatttatcat taccatgttg ccgaatggcg atctggtgcg
    15601 caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta tctaccgatg cgctggtcat
    15661 tgatatgtcc accatccatc cgctgcaaac cgataaattg attgccgata tgcaagccaa
    15721 aggcttcagc atgatggatg ttccggtagg ccgtacttct gcaaatgcca ttaccggtac
    15781 tctgttactg ctggctggcg gcaccgctga acaagttgaa cgtgccacac cgatcctgat
    15841 ggcgatgggc agtgagttga tcaacgctgg cggtccgggc atggggatcc gcgttaagct
    15901 catcaacaac tacatgagca tcgcgctcaa tgcgctttcg gcagaagccg ccgttttgtg
    15961 cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg
    16021 taaaggtcac ttcaccactt cctggccaaa caaagtcctc agcggagatc tttctcccgc
    16081 cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg ccaaccagct
    16141 gcatgtgcca atgccgctgg gggccgcctc acgggaggtt tatagccagg cgcgcgcatc
    16201 gggcggtttt gccatgctgg cggtcgatca gcgcgaagcc atgcgcatga tgtttgccgc
    16261 ggctggggca cccgctccgg tagccgatag cgttttaact gatttcaaag ttaacgctgc
    16321 aaaggccctc tcgccttatg cctcggcgat tctggtagat caacaattct gctaccgcca
    16381 ggtggttgag caaaacgcga ttgccaaaag ttgcgccatg attgtcgccg ccgatgagtt
    16441 cattcctggc aacggtattc cggtcgatag cgtggttatt gaccgcaaaa tcaatccgct
    16501 acagatcaaa caagacggtg gcaaagcctt aaaactgctg gtgctgtggc gtagtgatga
    16561 agatgcgcag caacgtctgg atatggtgaa agagttcaac ggactgtgcc actcacacgg
    16621 tctggtaagc atcattgagc cagtcgtccg cccaccgcgt cgtggcgata aattcgatcg
    16681 cgaacaagcg gtcatcgatg ccgccaaaga gctgggcgac agtggcgctg acctctacaa
    16741 agttgaaatg ccgctttatg gcaaaggtcc gcaacaagag cttctctgtg cttcacaacg
    16801 tctgaatgac catatcaata tgccatgggt gatcctctct tccagtgtcg acgaaaaact
    16861 gttcccgcgt gccgtacgcg tggcaatgac ggcgggcgca tcgggattcc tggcaggccg
    16921 tgcagtctgg gcatcggtcg tcggtttacc agacaacgag ctgatgctcc gtgacgtttg
    16981 cgcaccgaaa ttacaacaac ttggcgatat cgtcgacgaa atgatggcta aacgccgcta
    17041 agatgaggat aaaaggaatg aaatggttta acaccctaag ccacaaccgt tggctggaac
    17101 aggaaaccga ccgcatcttt gattttggta aaaattccgt agtgccgact ggttttggct
    17161 ggttaggcaa taaagggcaa atcaaagaag agatgggcac ccatctgtgg atcacagctc
    17221 gtatgttgca cgtttattcc gttgctgcgg cgatgggtcg acctggcgct tacgcgttgg
    17281 ttgatcacgg tattaaagcc atgaacggcg cactgcgtga taaaaaatat ggcggctggt
    17341 atgcctgcgt gaatgacgag ggcgtggtgg atgcctccaa acagggctat caacacttct
    17401 ttgctctgct gggtgccgcc agcgccgtca caacgggtca cccggaagcg cgcaagctgc
    17461 tcgattacac cattgagatt atcgagaaat atttctggag cgaagaagag cagatgtgcc
    17521 tggaatcctg ggacgaagcc ttcagcaaaa ccgaagagta ccgcggcggc aatgccaata
    17581 tgcacgcggt ggaagctttc ttgattgttt atgacgtcac tcacgacaaa aaatggctgg
    17641 atcgcgcgat tcgcgtggct tccgtgatta tccacgacgt cgccagaaat aatcattatc
    17701 gcgttaacga acacttcgat acccagtggg atccgctgcc ggattacaac aaagataacc
    17761 cggcgcatcg cttccgcgcg ttcggaggta caccaggcca ctggatcgaa tggggccgtt
    17821 taatgctgca catccatgct gccctggaag cccgttgcga acaaccacca gcatggctgc
    17881 tagaagatgc caaaggtctg tttaacgcca ccgtgcgcga tgcctgggca cccgatggcg
    17941 cggacgggat tgtttatacc gttgactggg aaggaaaacc ggtggtccgc gaacgtgtac
    18001 gttggcctat cgtcgaagca atgggtacgg cctacgcgct ctacaccgtc actggcgatc
    18061 gccagtatga aacctggtat caaacatggt gggagtactg cattaagtac ctgatggact
    18121 atgaaaatgg ttcctggtgg caggagctgg atgcggacaa taaggtcatc accaaagtct
    18181 gggacggcaa acaggatatt tatcacctgc tgcattgcct ggtgatcccg cgtatcccgt
    18241 tagcgcccgg catggctcca gcggttgcag cgggtctgct ggatattaat gcgaaataac
    18301 tgatctgaaa atagggtgcg ggttttcccc tcaccctaac cctctcccca aaggggcgag
    18361 gggaccgtcc ggtgcagttt ttgggtttca catcagcctc acgggaggct gtatgtcgtt
    18421 aattaaggtg ccttgtatgc aaatcaccaa tatgcattgc agtggacaga ccgtttcact
    18481 tgccgcgggg gattatcacg cgaccattgt gaccgtcggt gctggtctgg ctgaattaac
    18541 ctttcagggt tgtcatttag tcatcccgca caaacccgaa gagatgccgc tggcgcatct
    18601 gggtaaagtg cttattccgt ggccgaaccg gatcgccaac ggctgttacc gttatcaggg
    18661 gcaggaatat cagttaccca ttaatgaaca tagttcaaaa gccgctattc acgggctgct
    18721 tgcctggcga gactggcaaa ttagtgaatt aaccaccacc agcgttaccc tgacagcttt
    18781 tctgccgccc agttacggct atccgtttat gctggcttca caagttgttt attcgctcaa
    18841 cgcacacacg ggtctttccg ttgagatcgc aagccagaat atcggcactg tcgcagcgcc
    18901 ttatggcgtg ggtattcatc cttatttaac ctgcaactta acatctgttg atgaatatct
    18961 gttccagctt ccggcaaacc aggtttatgc cgtcgatgaa cacgtaaatc cgacaacgct
    19021 tcaccacgtt gatgagctgg atttaaattt cacgcaggcg aaaaaaatag ccgcgacaaa
    19081 aatcgaccac acctttaaaa ccgctaatga tttgtgggaa atgaccatta cccatccaca
    19141 gcaggcgctt tcggtaagcc tttgttccga tcaaccctgg gtgcaggttt atagcggtga
    19201 aaaattacag cgtcagggac tggcagttga acctatgagt tgcccgccta acgcctttaa
    19261 ttcaggtatc gacttattat tactggaacc aggaaaaccg caccgcctgt tttttaatat
    19321 ttacggacag agaaaataac ttgtattgat tagcagtcaa aatcattgaa gaacatttca
    19381 ctcgctaaag gcaacattat gtatcttcaa tttattaagt taaaaacagc tcagcttctg
    19441 taaagttttt atatatcagt tttatgagaa tcattttaca taagcctgta ataacaggaa
    19501 ggcaacaatc tcgttctcaa ctctatggag attagttatg gatacgccac gtccacagtt
    19561 attagatttt caatttcatc agaataacga cagttttacc ctacattttc aacaacgtct
    19621 tattttaacc catagcaaag ataatccttg tttatggatt ggctcaggta tagcggatat
    19681 cgatatgttc cgcggtaatt tcagcattaa agataaacta caggagaaaa ttgcgcttac
    19741 cgacgccatc gtcagccagt caccggatgg ttggttaatt catttcagcc gtggttctga
    19801 cattagcgcc acgctgaata tctctgccga cgatcagggc cgtttattgc tggaactaca
    19861 aaacgacaac cttaaccaca accgtatctg gctgcgcctt gccgctcaac cagaggacca
    19921 tatctacggc tgcggcgaac agttttccta cttcgatctg cgcggcaaac cgttcccgct
    19981 atggaccagt gaacaaggcg ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga
    20041 ctgcaaagaa aatgcgggcg gcgactatta ctggactttc ttcccacagc ctacgtttgt
    20101 cagcacgcag aagtattact gccatgttga taacagttgc tacatgaact tcgactttag
    20161 cgccccggaa taccatgaac tggcgctgtg ggaagacaaa gctacgctac gttttgaatg
    20221 tgctgacaca tacatctccc tgctggaaaa attaactgcc ctgctgggac gccagccaga
    20281 actgcccgac tggatttatg acggggtaac gctcggcatt catggcggga cggaagtgtg
    20341 ccagaagaaa ctggacacca tgcgtaacgc gggcgtgaag gttaacggca tctgggcgca
    20401 ggactggtct gggatccgta taacctcttt tggcaagcgc gtgatgtgga actggaagtg
    20461 gaacagcgaa aactacccgc aactggattc acgcattaag cagtggaatc aggagggcgt
    20521 gcagttcctg gcctatatca acccgtatgt tgccagcgat aaagatctct gcgaagaagc
    20581 ggcacaacac ggctatctag caaaagatgc ctctggcggt gactatctgg tggagtttgg
    20641 cgagttttac ggcggcgttg tcgatctcac taatccagaa gcctacgcct ggttcaagga
    20701 agtgatcaaa aagaacatga ttgaactcgg ctgcggcggc tggatggctg acttcggcga
    20761 gtatctgccc accgacacgt acctgcataa cggcgtcagt gccgaaatta tgcataacgc
    20821 ctagcctgcg ctgtgggcga agtgtaacta cgaagccctt gaagaaacag gcaagctcgg
    20881 cgagatcctc ttctttatgc gcgccggttc taccggtagc cagaaatact ccaccatgat
    20941 gtgggcgggc gaccagaacg tggactggag tctcgacgat ggcctggcgt ccgtagtccc
    21001 ggcggcgctg tcgctggcaa tgaccggaca tggtctgcat cacagcgaca ttggcggtta
    21061 caccaccctg tttgagatga agcgcagcaa agagctgctg ctgcgctggt gcgatttcag
    21121 cgccttcacg ccgatgatgc gcacccacga aggtaaccgt cctggcgaca actggcagtt
    21181 tgacggcgat gcggaaacta tcgcccattt tgcccgtatg actaccgttt tcaccaccct
    21241 gaaaccttat ctgaaagagg ccgtcgcgct gaatgcgaag tccggtctgc cggtcatgcg
    21301 cccgctgttc ctgcattacg aagacgatgc acatacctat tccctgaaat atcagtacct
    21361 gttaggccgc gacattctgg tcgctccggt acacgaagaa ggtcgcagcg actggacgct
    21421 ctatctgccg gaggataact gggtacatgc ctggacgggt gaagcgttcc ggggcgggga
    21481 agttaccgtt aatgcgccca tcggcaagcc gccggtcttt tatcgcgccg atagcgaatg
    21541 ggcggcactg ttcgcgtcgt taaacagcat ctaatcacat ccgcccgtgg ggaaactcat
    21601 gggcaaaggg agaacatcga tgagtcacat cacaacggaa gatccagcaa ctttacgcct
    21661 gccctttaaa gagaaactct cttacggtat tggcgacctg gcctctaaca tcctgctgga
    21721 tatcggtacg ctttatcttt tgaagtttta taccgacgtt ctggggctgc caggcaccta
    21781 tggcggcatt atctttttga tttcaaaatt ctttactgcg tttaccgata tgggtaccgg
    21841 cattatgttg gattcccgac gcaagatcgg tccaaaaggt aagttccgtc cttttattct
    21901 gtatgcgtca ttcccggtca ccttactggc gatcgccaac tttgtcggca caccgtttga
    21961 tgtcaccggt aaaacggtga tggccactat tctgtttatg ctttacggac tgtttttcag
    22021 catgatgaac tgctcctacg gcgcaatggt cccggcaatc accaaaaacc ccaacgaacg
    22081 cgcctcactg gcggcatggc gtcagggcgg cgctacgctg ggcctgctgc tgtgcacggt
    22141 gggattcgtg ccagttatga atcttatcga aggtaatcag aaacttggct atatcttcgc
    22201 cgccacgctg ttttcactgt tcggcctgct gtttatgtgg atctgctact caggcgtgaa
    22261 agagcgttat gtcgaaaccc aaccaaccaa tccggcgcaa aagcctggcc tgttgcaatc
    22321 tttccgcgca attgccggta accgcccact gttcattctg tgtattgcca acctctgcac
    22381 cttaggggcg tttaacgtca agctcgccat tcaggtctat tacacccagt acgtacttaa
    22441 cgatcccatc ctgttgtcgt atatgggatt tttcagcatg ggctgtattt tcatcggcgt
    22501 gttcctgatg cctggcgcag tcagacgttt tggtaagaag aaggtctata tcggcggcct
    22561 gctgatttgg gtgctgggcg atctgctcaa ctatttcttc ggcggcggct cggtcagctt
    22621 tgtggcgttc tcctgcctgg cgttcttcgg ctcagcgttt gttaacagcc tgaactgggc
    22681 gctggtttcc gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt cggaaggcac
    22741 ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc
    22801 cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg accacaccat
    22861 tgaagggtta cgccagttga tcttcatcta cccaagcgca ctggcggtag tcaccattgt
    22921 ggcgatgggt tgcttctaca gcctgaacga gaagatgtat gtccgcattg tggaagagat
    22981 agaagcccgt aaacgcacgg cgtaataata ataaacgacg ccctgcgggg cgttataagg
    23041 agtggttatg tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc
    23101 ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc atcggtactc tgtatctcct
    23161 caaactttat accgatgaat tagggatgcc tgcttactat ggcgggatta tctttctggt
    23221 cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa
    23281 aaatattggg cctaaaggca aatttcgtcc ctttatttta tatgcagcag ttccggcggc
    23341 gttaattgca acgctccagt ttatcgccac caccttttgt ttgccggtta aaacgacgat
    23401 tgccaccgcg ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg
    23461 tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg
    23521 ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc
    23581 gcttttttct gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg
    23641 ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcgcc
    23701 aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgta atccaccatt
    23761 gctggtttta tgcattgcta acctgtgtac tctggcggca tttaatatca aactggcgat
    23821 tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt
    23881 cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccagtaactg taaaatgttt
    23941 tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggtgggtg atatactgaa
    24001 ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg
    24061 cacggccttt gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga
    24121 atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa
    24181 aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt
    24241 tcccaacatc gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg
    24301 gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga
    24361 aaaacggttt gcattgatta ttgaggaaat caaccaacga aaaaataaag agatggcgac
    24421 agaaaaaaaa acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt
    24481 gggtggcggc ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa
    24541 ttttattatc ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg
    24601 aggcatataa tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg
    24661 atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa
    24721 aacatggcta taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa
    24781 ccatccagcc cggtggctt
//
LOCUS       DADLTI010000048        24614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLTI010000048 DADLTI010000000
VERSION     DADLTI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.048"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            238..1890
                     /locus_tag="JN755_001766"
     CDS             238..1890
                     /locus_tag="JN755_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7380588.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(1927..2406)
                     /gene="ybaK"
                     /locus_tag="JN755_001767"
     CDS             complement(1927..2406)
                     /gene="ybaK"
                     /locus_tag="JN755_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7380589.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(2610..3404)
                     /locus_tag="JN755_001768"
     CDS             complement(2610..3404)
                     /locus_tag="JN755_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380590.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            3542..3883
                     /locus_tag="JN755_001769"
     CDS             3542..3883
                     /locus_tag="JN755_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7380591.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4098..6602)
                     /gene="copA"
                     /locus_tag="JN755_001770"
     CDS             complement(4098..6602)
                     /gene="copA"
                     /locus_tag="JN755_001770"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7380592.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            6864..7796
                     /gene="glsA"
                     /locus_tag="JN755_001771"
     CDS             6864..7796
                     /gene="glsA"
                     /locus_tag="JN755_001771"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7380593.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            7799..9091
                     /locus_tag="JN755_001772"
     CDS             7799..9091
                     /locus_tag="JN755_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7380594.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            9216..9623
                     /locus_tag="JN755_001773"
     CDS             9216..9623
                     /locus_tag="JN755_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7380595.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(9624..10082)
                     /locus_tag="JN755_001774"
     CDS             complement(9624..10082)
                     /locus_tag="JN755_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7380596.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(10079..10996)
                     /locus_tag="JN755_001775"
     CDS             complement(10079..10996)
                     /locus_tag="JN755_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7380597.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11142..11819
                     /gene="fetA"
                     /locus_tag="JN755_001776"
     CDS             11142..11819
                     /gene="fetA"
                     /locus_tag="JN755_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7380598.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGY
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            11806..12585
                     /gene="fetB"
                     /locus_tag="JN755_001777"
     CDS             11806..12585
                     /gene="fetB"
                     /locus_tag="JN755_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7380599.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12648..13502)
                     /gene="cnoX"
                     /locus_tag="JN755_001778"
     CDS             complement(12648..13502)
                     /gene="cnoX"
                     /locus_tag="JN755_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7380600.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(13563..14372)
                     /gene="ybbO"
                     /locus_tag="JN755_001779"
     CDS             complement(13563..14372)
                     /gene="ybbO"
                     /locus_tag="JN755_001779"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7380601.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(14362..14984)
                     /gene="tesA"
                     /locus_tag="JN755_001780"
                     /pseudo
     CDS             complement(14362..14984)
                     /gene="tesA"
                     /locus_tag="JN755_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            14955..15641
                     /locus_tag="JN755_001781"
     CDS             14955..15641
                     /locus_tag="JN755_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7380602.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            15638..18052
                     /locus_tag="JN755_001782"
     CDS             15638..18052
                     /locus_tag="JN755_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7380603.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(18764..19858)
                     /gene="mnmH"
                     /locus_tag="JN755_001783"
     CDS             complement(18764..19858)
                     /gene="mnmH"
                     /locus_tag="JN755_001783"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7380604.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(19927..20853)
                     /gene="allS"
                     /locus_tag="JN755_001784"
     CDS             complement(19927..20853)
                     /gene="allS"
                     /locus_tag="JN755_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7380605.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            21083..21565
                     /gene="allA"
                     /locus_tag="JN755_001785"
     CDS             21083..21565
                     /gene="allA"
                     /locus_tag="JN755_001785"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7380606.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            21643..22458
                     /gene="allR"
                     /locus_tag="JN755_001786"
     CDS             21643..22458
                     /gene="allR"
                     /locus_tag="JN755_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7380607.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            22548..24329
                     /gene="gcl"
                     /locus_tag="JN755_001787"
     CDS             22548..24329
                     /gene="gcl"
                     /locus_tag="JN755_001787"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7380608.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            24342..>24614
                     /locus_tag="JN755_001788"
     CDS             24342..>24614
                     /locus_tag="JN755_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7380609.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT         5670 a         6310 c         6651 g         5983 t
ORIGIN      
        1 gtttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat
       61 cttatttgtg attattaccc gactaacata cctgtatgcg tcgtctgaag gaagtctcaa
      121 cgccgaatac agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtc
      181 tatgatgtcg cgtcatacta ttttttaaca cgttgaaatc aggtcaggga gagaagtatg
      241 aaattattgc agcggggcgt ggcgttagcg ctgttaacca catttacact ggcgagtgaa
      301 actgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca taccaatgat
      361 catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg
      421 ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc
      481 ggtggcgaca ttaacactgg cgtgcccgag tctgacttac aggatgccga acctgatttt
      541 cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataat
      601 ccgctcaccg tattacgcca gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat
      661 atctaccaga aaagtactgg cgagcgcctg tttaaaccgt gggcgctgtt taagcgtcag
      721 gatctgaaaa ttgccgttat tgggctgaca accgatgaca cagcaaaaat tggtaacccg
      781 gaatacttca ctgatatcga atttcgtaag cccgccgatg aagcgaagct ggtgattcag
      841 gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac
      901 gataatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct
      961 gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg catggcggca
     1021 gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccaga tcaacaaaac
     1081 ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgattttgag
     1141 tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag
     1201 aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa
     1261 aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc gcagctggaa
     1321 gtgaaaatag gcaaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta
     1381 cagaccaata tggggcggtt gattctggca gcccaaatgg atcgcactgg tgccgacttt
     1441 gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggcgatat cagctataaa
     1501 aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat gaccggtaaa
     1561 gaggtgattg attacctgac cgccgtcgcg cagatgaagc cagattcagg tgcctacccg
     1621 caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct taaaatcaaa
     1681 ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga cattaaactt caatgccacc
     1741 ggcggtgatg gatatccgcg ccttgataac aaaccgggct atgtgaatac cggctttatt
     1801 gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt gagtgtttat
     1861 gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg ccggatgttt gcatccggca
     1921 caatgcttaa tcgcggcggg cgatatcagc aaatttggca tcgaggatct ttgccagatc
     1981 gcctgccgcc agttcgatat ccagtccgcg cttgccgccg gaaacataaa tagtggcaaa
     2041 ttcttgtgcg ggggcgtcga taatcgtggg cagacgtttt ttctgcccca gtgggctaat
     2101 ccccccaacc aggtatcccg tcgaacgctg cgcgaccatc ggatcggcca tctcaacttt
     2161 cttggcaccc agcgcttttg ctactttttt aagatccagt tgacctgcga ccggcgtaac
     2221 ggccacggca aggtgtttca tatcaccgtt cactgccacc agcagcgttt tgtagacctg
     2281 atccggattc aaacctaatt ttttgacgac ttcatcgcca aaattggttt cagccggatc
     2341 gtgctcgtag gtatggattt gaaacgaaat cttgtttttt tcgagtaatt taactgcggg
     2401 tgtcatggta attttcctgc cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg
     2461 tccaaaaaaa agagtcatct tgcctaagag tattggcagg atggtgagat tgagcgacaa
     2521 tcgagttaca ccgtcgctta aagtgacggc ataataataa aaaaatgaaa ttcctctttg
     2581 acgggccaat agcgatattg gccatttttt tagcgcaaca tttgcggcaa attcccttct
     2641 ccatacaggt gtagtgcacc gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat
     2701 ttatcccgcc aggcgagatt tcgctgatgc atcagcacat cgtacagcga ctgactgaac
     2761 gtattgggca gcgttatatc attattttgc ggcggtgcat tcagccacca gctcatcatt
     2821 tgttgcagca accgtgcgtt ggtatgccag tgggtcagcg tatcgtccag cagcgccagt
     2881 cctttgtcag ggagctggag caacatggca atctggtttt cagccccttc cagttcaatc
     2941 acgggtttat gttgttgctt cgccgcctgc aatagctggt aatcgatacc gtattctgcc
     3001 cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt
     3061 tgggtagaaa agagtgaggg agaaatgccc atctcctggc taatgtgctg caggttttgg
     3121 agttgctcct cgctaatgcg ctcttccagc gcctcgcagg caggcaaatt agcaaaaggc
     3181 gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat cggcgttttt gagctttttg
     3241 agcaaacggg tgggcagggg agccatatcg tggctaccca tatgaatact gccaatcaga
     3301 tgaaaatggc gattgccggg gagggtgata tcgatggcag gccaggtgta atgattaccg
     3361 cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat ccatacgacc tccctttgtg
     3421 aaatatcatg ctagcgcgcg gtgagggatg gcgcaaatgg agttggggac ttgtttaatg
     3481 tgtttgtatg attcagtatg ttcttgcatc gctattcaca gggaagcaac agttaaaaac
     3541 catgaaacag gcaacaagaa aaccgactac acccggagat attcttctct atgaatatct
     3601 ggaaccgctc gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatcg
     3661 cgtcagtgca ctgatcaata acaatcgtaa actcaccact gagatggcat tccgtctggc
     3721 gaaagttttt gataccacag tcgattttag gctaaacctc caggcggcag ttgatctttg
     3781 ggaagttgaa aacaacatgc gcacccagga agaattggga cggattgaaa cagtggctga
     3841 atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc
     3901 atctcgtagg ccggataagg cgttcacgcc gcatccggca tccgagcacc attgcctgat
     3961 gcgacgctta cgcgtcttat caggcctaca aacctgtgcc tgaaccgtag gccggataag
     4021 gcgtttacgc cgcatccggc agtcatgcgt cgatgccaaa tgcgccgccc taaagcagcg
     4081 catccgcaat gacgtactta ttccttcggt ttaaaccgca gcaaccggtt ggcgttactc
     4141 actacggtaa tcgacgagag cgccattgcc gctccggcaa ctaccgggtt aagcagtgtt
     4201 ccagtaaacg gccacaaaat accggcggcg accggaatac cgatgctgtt gtagataaag
     4261 gcaccgagca ggttctgctt catgttgcgc agcgttgcgc gggaaatcgc gagcgcatcc
     4321 gcaacgccca tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat ggcaacatca
     4381 ctgccgccac ccatcgcaat gccgacatcc gcctgggcca gcgctggcgc gtcgttaatg
     4441 ccgtcgccca ccattgccac ctgacgtcct tcactttgca gacgtttgat cgcttcggct
     4501 ttaccgtccg gcagcactcc ggcaatcact tcatcaattc cagcttcttt ggcgatcgca
     4561 ttggcggtgg ttgggttatc cccggtcaac atcaccagac gatatcccgc tttatgcagg
     4621 cgttgcagcg ccgccacgct atcactacgc aacggatcgc gtacagccag cagggctact
     4681 gctttcccgt caatcgccag cagcacaggc gttgctcctt gcgatgcctg agctgaaata
     4741 tcggcttcaa tcgctttagt atcgacttgc tgatcattta acaacgcctg attacccagc
     4801 aataacgcat gaccttctgc ttcaccgctc acgcccagcc cgcgcaatgt gcggaaaccg
     4861 ttgacctgcg gtagctgcat atcgcctgct ttatcaagga tcgctcgtgc caatggatgg
     4921 ctggaacctt gctccagcgc cgccgccaga cgcagcgcct gcgcttcgtc aacatcagca
     4981 aatgttttca ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac
     5041 acgacggtat cgagtgtact ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca
     5101 aactcagccg cccgcccgac gccggaaata atcgacatcg gcgtcgccag tcccagcgca
     5161 cacgggcagg cgataatcag caccgtagtg gcaatcacca gggtatagac aatctgcggt
     5221 gccggaccaa agaaatacca gattgccgca ctgacaagcg caataaccac tactaccggc
     5281 acaaatacgg ctgagatttt atccgccagc tgaccgattt ctggcttgct gctctgggcc
     5341 tggcgcacca tgcgaatgat ccttgatagc gtggtatggc tgccaaccgc actggcacga
     5401 aacagcacac tgccgtcctg taccactgtc ccggcatgga cgctatcgcc ttcgcctttt
     5461 tgctgcggga ttggttcgcc cgtcagcatc gcttcatcca gccatgcttc gccctgggta
     5521 atctcgccat ctaccggcac gcgatcgccg gtcgtcaggc gcagcaacat acctggctgc
     5581 acttctgcca gaggcacgct tttttcacct tcgtcagtaa ccaggcgcgc cgtcggtggg
     5641 gttaaatcga gtaatttctc cagtgcctta gaagaacgct gacgtgcgcg tgcttccagc
     5701 atatggccga gattgatcag accgataatc atcgcactgg cttcatagta aagatgtcgc
     5761 gcttccatcg ggaaccactg cggccacagg ttgacgctca tcgaatagag ccacgccacg
     5821 cccgttccca gcgccaccag cgtatccatc gtcgctgcac cgttcagcag gcttttccat
     5881 gcactgcggt aaaaatggcc gccggcgaaa accatcaccg ccagggttat caggccgata
     5941 accaaccaca ggctgcggtt gtcggcggtg accatcatgt tatcgccgat catcccccag
     6001 accatcaccg ggatacccac cgccagtgcg acaattgcct gccagcggaa gcgcttcatc
     6061 gtagcgacgg cggtttcttg ctggcgttcg cggcgtttcg cgtcatcttc aatcgcttcc
     6121 gcgccgtagc ccgctttttc caccgcctgc actaaatctt gtggggaggc actgcccatc
     6181 accagcgcag tacgctccgc caggtttacc cgtgcctgag tgacgcccgg tacgctttgc
     6241 agcgcatttt gtacgcgggt gacacagctg gcgcagctca tgccgctcag cagcaactgc
     6301 tggctgtcat cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct
     6361 tccgacggga ttgatgactc cgccagcggt ttagcctttg ggtggcttac agatgcgtca
     6421 taacccgctt gtttgatggt ttcgatcagc tgttctgcac tggcagtccc ggtaacgtgc
     6481 gcttcagtga tagacacatc cgcttgttcc acatccgggc gctgttcaag actttctttc
     6541 acgcgtttaa cgcagtgacc gcaggacagg ccgtccaggt tcaggtcgat agtttgtgac
     6601 ataaaacact cctttaagac agttttgact ggctgtgata aaggttaaac cttccagaaa
     6661 ggggaaggtc aagaaattaa taaaccaggc gggtaaaagt ccgtaaagat taaaaaatcg
     6721 gctcgatttg catcaggatt agacatttat ttctttgttt tcctgtagtt aagttgcggg
     6781 tgctaagtta aatcaggatg cctgaaaatc ggcacagggg tgaggaatta cctcccgcat
     6841 ctataaaaag gagttaacaa aagatgttag atgcaaacaa attacagcag gcagtggatc
     6901 aggcttacac ccaatttcac tcacttaacg gcggacaaaa tgccgattac attccctttc
     6961 tggcgaatgt accaggtcaa ctggcggcag tggctatcgt gacctgcgat ggcaacgtct
     7021 atagtgcggg tgacagtgat taccgctttg cactggaatc catctctaaa gtctgtacgt
     7081 tagcccttgc gttagaagat gtcggcccgc aggcggtaca ggacaaaatt ggcgctgacc
     7141 cgaccggatt gccctttaac tcagttatcg ccttagagtt gcatggcggc aaaccgcttt
     7201 cgccactggt aaatgctggc gctattgcca ccaccagcct gattaacgct gaaaacgccg
     7261 aacaacgctg gcagcgaatt ttacatatcc aacagcaact ggctggtgag caggtagcgc
     7321 tctctgacga agtcaaccag tcggaacaaa caaccaactt ccataaccgg gccattgcct
     7381 ggctgctgta ctccgccgga tatctctatt gtgatgcaat ggaagcctgt gacgtgtaca
     7441 cccgtcagtg ctctacgctc atcaatactg ttgaactggc aacgcttggc gcgacgctgg
     7501 cggcgggtgg tgtgaatccg ttgacgcata aacgcgttct tcaggccgac aacgtgccgt
     7561 acattctggc cgaaatgatg atggaagggc tgtatggtcg ctccggtgac tgggcgtatc
     7621 gcgttggttt accgggcaaa agcggtgtag gtggcggtat tctggcggtc gtccctggcg
     7681 tgatgggaat tgccgcgttc tcaccaccgc tggacgaaga aggcaatagt gttcgcggtc
     7741 aaaaaatggt ggcatcggtc gctaagcaac tcggctataa cgtgtttaag ggctgatcat
     7801 gatgaacacg gaaggtaata acggtaacaa acccctcggt ctatggaacg tcgtttccat
     7861 cggtattggg gcaatggtgg gggcggggat cttcgcgctg ctggggcagg ctgcgttgct
     7921 aatggaagcc tcgacctggg gcgcctttgc ttttggcggt attgtggcga tgttttccgg
     7981 ttatgcctat gcgcgtctgg gggcgagcta tcccagcaat ggcggtatta tcgacttttt
     8041 tcgtcgcgga ttaggcaacg gcgtcttttc gctagcgctc tcgttgctat acctgttgac
     8101 gctggcggtg agcatcgcca tggttgcccg tgcttttggc gcttatgccg tgcagttttt
     8161 gcatgaaggc agccaggagg agcaccttat tttgctctac gcgttgggga tcattgcggt
     8221 gatgacgctt ttcaactcct taagcaacca tgcggtaggg cggctggaag tgatcctcgt
     8281 cggcattaaa atgatgatcc tgttattgct gattattgcc ggtgtctggt cgctgcaacc
     8341 ggcgcatatt tccgtctctg cgccccccag ctccggtgcg ttcttctcct gtattgggat
     8401 aaccttcctt gcctatgcgg gctttggcat gatggcgaac gcggcggata aagtgaaaga
     8461 tccgcaggtc attatgccac gggcgtttct ggtggcgatt ggcgttacca cgttgcttta
     8521 tatctcgctg gcgctggttt tgcttagcga tgtatcggca ttagagttag aaaaatatgc
     8581 tgataccgcc gtagcgcagg ctgcttctcc gctgctcggg catgtgggtt atgtgatcgt
     8641 cgtcatcggc gctttactgg cgacggcttc aggcattaac gcgaacctgt tcgccgtgtt
     8701 taacatcatg gacaacatgg gcagcgaacg cgaactgccg aagctaatga ataaatccct
     8761 gtggcggcag agtacctggg gcaacatcat cgtcgtggtg ttgataatgc tgatgacggc
     8821 ggcgctaaat ttagactcac tcgccagcgt tgccagcgcc acctttttga tctgctatct
     8881 ggcggtgttt gtggtggcga tccgcctgcg tcatgatatt cacgcctcgt tgccgattct
     8941 tatcgttggt acgttggtga tgttgttggt gatcgttggc tttatctaca gcctgtggtc
     9001 ccagggcagc cgtgcgttga tatggattat tggtgcaatc ttactcagcc ttattgtggc
     9061 aatggtcatg aagcgtaata aaaccgtata acacctctct gtgcgcagta cttcctgtat
     9121 tattgtggtg gcggtcgata ttcgcactgg caaaaaaacg tgcttgaata tctgttgaaa
     9181 ccctttaaca aagcacagga ggcgttgcgc gaacgatgaa catcagcgat gtagcaaaaa
     9241 ttaccggcct gaccagcaaa gccattcgct tctatgaaga gaaggggctg gtgacgccgc
     9301 cgatgcgcag cgaaaacggt tatcgcacct acacgcagca gcatctcaac gaactgacct
     9361 tactgcgcca ggcacggcag gtgggcttta acctggaaga gagcggcgag ctggtgaatc
     9421 tgtttaacga cccgcagcgg cacagcgccg acgtcaaacg gcgcacgctg gagaaggtgg
     9481 cggagatcga acgacacatt gaggagctgc aatccatgcg cgaccagctg ctggcactgg
     9541 cgaatgcctg ccctggcgat gacagcgccg actgcccgat tatcgaaaat ctctccggct
     9601 gctgtcatca tcgggcaggg tgattaagac gagacggctc ggatatgtag ggttatccct
     9661 tctatcgcaa tgacttcaac atacgtacct gcaccgagat cctcgctggc gctgacaggc
     9721 catgaactgt caccgacgcg catatgaccg cgcccgttga ccagcggaga ttccagcaca
     9781 aaacgtcggc caatcagctg ctgcccgcgc tggtttaaat gactatcgct gtgcttttgt
     9841 tcgcgcaccc gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggatg
     9901 gcaaacatta ccccttgcca ctcccaaccc agcggcacca gccagaccac caggccggta
     9961 atcaccgctg ccacgccgct ccacaacaaa taaccatttc cgcccagcat ctcggctgcc
    10021 agcagcaacc cgccgagact gagccagaaa atatgtggat gaacgaccat taactccatc
    10081 atggctgagt ccgtttgttg gcgctgtctt tcaccagctc ggcaatcccg gcaatcgacc
    10141 ccatcaggct gctggcctct aatggcatca tcactacttt gctgttactg gaggaaccga
    10201 tctgctgtaa cgcttcggtg tatttttgcg ctacgaagta gttcaccgcc tgaatatcac
    10261 cggaggcgat agcttcagac accattttgg tggcgcgggc ttctgcttcg gcggaacgtt
    10321 cacgcgcttc agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgcg
    10381 actgtttttc accttcggct ttgaggattt ccgcctgacg gatcccttcc gcttcaagaa
    10441 tgtaagcgcg tttggtacgt tccgctttca tctgcgcgtt cattgaagag ataagctctg
    10501 ccggtgggcg cacgtcgcga atttcaatac gggtgacttt aatcccccac gggttggtgg
    10561 cttcatcgac aatacgcagc aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt
    10621 caagttccat tgaacccaac acggtacgga tgttagtcat ggtcaggttg atgatcgcca
    10681 gctccagatt gctgacttca taggcagcgc gtggcgcgtc aatcacctga ataaaacaga
    10741 ctgcgtcgat ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatatcga
    10801 gcacttgctc catcatattg atcttgcgac caatgcgatc cataaacggc accaccagac
    10861 tgagccccgg ctgtaacgtt ttggtatagc gaccaaaacg ttctaccgtc cattgatagc
    10921 cctgcggcac gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg
    10981 ggataaagat aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct
    11041 gcttgattaa acaaattata cctgattact gaaagagagt tcccccttat tcctgcgaag
    11101 gataaactgt ttttagtaaa aatcagaaaa agggaacagc gatgcaggaa aatagtcctt
    11161 tgcttcagct acaaaacgta ggatatctgg cgggtgatac gaagattctt aataacatca
    11221 atttttcgct gcgtgctggc gaatttaagt taattaccgg tccttctggt tatggcaaaa
    11281 gtacgctgct aaaaatagtt gcttcattga tcagcccaac cagcggaacg ttactgtttg
    11341 aaggtgagga tgtcagcaca ctaaaaccag aaatctaccg ccagcaagtc tcttactgcg
    11401 cccagacgcc gacgctgttt ggcgatacgg tatacgataa tctgatcttt ccctggcaga
    11461 tccgtgacca gcagcctgac ccggccattt ttctcgattt tctcgaacgc ttcgctttgc
    11521 cggacagcat tttgacgaag aatatcgccg agctatctgg tggtgaaaaa caacgcatct
    11581 cattgattcg taacctacaa tttatgccga aggttttatt gctggatgaa ataaccagtg
    11641 cgctggatga aagtaataaa cataacgtca atgagatgat ccatcgttat gtgcgcgagc
    11701 aaaatattgc cgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataaag
    11761 tgattacact gcaaccgcat gccggagaaa tgcaggaagc acgctatgaa ctcgcataat
    11821 attactaacg aatcattagc actggcatta atgctggtgg tggtggcaat cttaattagc
    11881 cataaagaaa aactggcgct ggagaaagat attctctgga gcgtcgggcg agcgataatt
    11941 cagctgatta ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg
    12001 acattattga tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt
    12061 aaatatattg ctaaagcttt tatctcatcg tttattgcca ttacggtcgg ggcgggaatt
    12121 accctggcgg tgctgattct ctcagggtcg attgaattta tcccgatgca ggtgatccct
    12181 gtcgccggga tgattgccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta
    12241 gggcaacggg tcattagcga acagcaacag attcaggaga aactgagtct tggcgcgacg
    12301 ccgaagcagg cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg
    12361 gtcgattcag caaaaacggt tggcttagtg agtttaccag gaatgatgtc cgggctgata
    12421 tttgccggga ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg
    12481 ctctcaaccg ccagcttgtc gaccattatt gcctgctatt taacctatcg taagttttat
    12541 aattcgcgcc accagttggt ggtgactcaa ttgaagaaga aatgatgcga tgccggatgc
    12601 gcagcatcgc atccggcgtt gtggttcatg tgccgaacaa cggaatatca atacaacaat
    12661 gcatacagct ggcggcgata cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg
    12721 atctcctgga acgttttacg cgtctgaccg tctgcggcgg tgagatcttt acgcagatgc
    12781 ccgaacagca actccagcgc ctcttcattg cgcccaacct gatgcagttg cagcgccagt
    12841 tgcgtcgcca gtgcggcatc ttctggattc tccgccacct gctgttgcaa ctgttgaatt
    12901 tccggcttat cagccgcctg cttcagcagt tcgatttgcg ctaccagccc ctggtagcgg
    12961 gtgtcctgat cctgcaacgg aatggttttc agcaccgctt ccgcatcttc agaacggttc
    13021 agcgcaatca gcgtttctgc cagcagcagg ccgatctccc cgttctgatt cgacaactgc
    13081 caggcgtctt tcagcaatgg cagggcatcg gtgtaattgc cttcctgcat cagttgcatc
    13141 gcctgctgcg ctttcagctc ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc
    13201 gcctcttccg gttgcggccc ctggaagcca tctaccggtt gcccgttctg gaacagatac
    13261 acggtcggaa tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc
    13321 agcttcgcca gaataaattg cccgttgtac tgcgccgcga ggctttccag gatcggggtt
    13381 aactgcaaac agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc
    13441 gactgttcaa gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac
    13501 atggagtcgc tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca
    13561 actcacccct gcaatatttt gtccatcacg cgcccaggca gcaggcgctc gagcaccatt
    13621 actgcccagg tcaccagcgt taccggatag cgcatcttcg gcttctcgct aataaaagca
    13681 tggcgcactt tgtcgaccac cgcttccggt cccaacgtaa agcgggcggc gatgccggga
    13741 ttttcgactg gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg
    13801 ggaccgggtt cgatcaggct gactttaatt ccgctgtggc gtagctccat gcgcagcgcg
    13861 tctgaccacg cctccagcgc atatttactg gccgcgtaag cgccacgacc cggcgtggag
    13921 attaatccca tcaccgatga tgtcatcaca atacgccctt caccgtgcgg taacatcgcg
    13981 ggtaacaggc gcatggtgag ctggtgtgcg ccgaaaaagt tggcggaaaa ctgctgttcc
    14041 atctgcgcac ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag
    14101 atcccataca gacaattatc ggtcagggcg atcacctcgt ctgctgcgcg attaacactt
    14161 tctggtgaat ccagatcgat caacacgccg gtaaatccca tgttgttcat acgctcaaca
    14221 tcattcggtt ttcggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg
    14281 ctttccaggc caattccact ggaacatccg gtaattaaga ccgatttttg cataacttta
    14341 cctgtcagga tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc
    14401 catccagtcg gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat
    14461 ccattgtggc ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc
    14521 tttggcgagt ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc
    14581 aggcagacgt atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat
    14641 ctggcgcagc gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccat cattgcgccc
    14701 agttcaacca gcacccaacg cggctgatgc tgtttgagca gagccggaag gcgcgccagc
    14761 ccttgttgcg aggtgtcgcc gctgatgctg gcattaacta ccgacgtttt actctgccac
    14821 ttatcattca acaaggcagg ccaggccgcg ctggcagaca ttcgatatcc ggcgctcagg
    14881 ctatcaccca gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa caggaccagg
    14941 aacaggaagg gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg
    15001 tcggtcaggg ggagcatgaa ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg
    15061 gcgagaccat cgcactggtg ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc
    15121 tcgccgggct tgatgacggc agcagtggcg aagtgagtct ggtgggacaa ccgctacata
    15181 atatggacga agaagcgcgg gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt
    15241 catttatgtt aattcctacc cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc
    15301 gcggtgagag tagcgcggaa agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc
    15361 tgggtaaacg tctggatcat cttccggcac agctttccgg cggtgaacag caacgagtgg
    15421 cgctggcacg agcctttaat ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca
    15481 accttgaccg ccagacgggc gataaaattg ccgacctgct gttttccctc aaccgtgaac
    15541 atggcaccac gttgattatg gtgacccacg acctgcaact ggcggcacgc tgcgaccgct
    15601 gcttacggct ggtgaacggg cagttgcagg aggaagcatg attgcacgtt ggttctggcg
    15661 cgaatggcgt tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg cggtggcctg
    15721 cgtgctggcg ctgggcaata tcagcgatcg catggagaag ggcttaagcc agcaaagccg
    15781 tgagtttatg gcgggcgatc gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct
    15841 ggaggaagcg caaaagcgcg gcctgaaagt cggcaagcag ctgactttcg ccacaatgac
    15901 ctttgcaggc gacacaccgc agctggcgaa cgtcaaagcg gtggatgata tctacccgat
    15961 gtatggcgat ctgcaaacta atccccctgg cctgaaaccg caggcgggca gcgtattgct
    16021 ggccccacgc ctgatggcac tgcttaacct gaaaacggac gacaccattg acgtgggcga
    16081 tgccaccttg cggattgccg gagaagtgat tcaggaaccg gattccggtt ttaacccctt
    16141 ccagatggct ccgcgtctga tgatgaatct ggcggatgtc gataaaaccg gagccgtgca
    16201 gccggggagt cgggtcacct ggcgttataa attcggcggc aacgagaacc agctcgacgg
    16261 ctatgagaaa tggttgttac ctcagcttaa acccgaacaa cgctggtacg gtctggaaca
    16321 ggacgaaggc gcgctggggc gatcgatgga acgctcgcaa cagttcctgc tgctttcggc
    16381 gcttctgacc ttgctgctgg cagtggcagc ggtggcggta gcgatgaatc attactgtcg
    16441 cagtcgctac gatctggtgg cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg
    16501 taagctaatc gtcggtcagt ggttgatggt gctgacgctt tcagccgtta ccggtggggc
    16561 cataggcctg ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc
    16621 actaccgcca gccagcctct ggccgtggct gtgggcgctt ggcaccatga cggtcatctc
    16681 gctgctggtg gggctacgac cataccgctt gttgctggca acgcagcctt tacgcgtatt
    16741 acgtaatgat gtggtcgcga acgtctggcc gctgaagttt tatctgccga ttgtcagtgt
    16801 ggtggttgtg ctgctgctcg ccggattaat gggtggcagc atgctgcttt gggcggtgct
    16861 ggcgggcgcg gtagtactgg ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt
    16921 acttcgccgc atgacgctga aatcgctgcc tctgcgcctg gcggttagcc gcctgttacg
    16981 tcagccgtgg tcaacgttaa gccagctttc ggcattttcg ctctccttta tgctgctggc
    17041 actgctgctg gtgctgcgtg gcgatctgct cgaccgctgg cagcagcagc taccgccaga
    17101 aagcccgaac tactttttaa ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt
    17161 cctcgcggaa catcatatag tcccggaatc attttatccg gtagtgcggg cgcggctgac
    17221 ggcgattaac gataagccga cagaaggtaa tgaagatgag gcgcttaatc gcgaactcaa
    17281 tcttacctgg caaaatacgc ggcccgatca taatccgatt gtcgccggta actggccgcc
    17341 aaaagccgat gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg
    17401 tgataccgtg acttttatgg gcgataccca ggagttccgc gctaaagtga ccagcctgcg
    17461 caaagtggac tgggaaagtc tgcggcctaa tttctatttt attttccctg aaggggcatt
    17521 agacgggcaa ccgcagagct ggcttaccag tttccgctgg gagaatggca acggcatgtt
    17581 gacgcaactc aaccgccagt tcccgaccat tagcctgtta gacattggcg cgattttaaa
    17641 acaggtcggt caggtgctgg agcaggtaag tcgggcgctg gaggtgatgg tggtgctggt
    17701 caccgcctgc ggtatgttgc tgttgctggc acaggtgcag gtgggaatgc gccagcgtca
    17761 tcaggagctg gtggtatggc gcacactcgg tgcggggaaa aaactgctgc gcacgacatt
    17821 gtggtgtgag ttcgccatgc ttgggtttgt ttccggcctg gtggccgcaa ttggtgcgga
    17881 aacggcactg gcagtgttgc aggcgaaagt gtttgatttc ccgtgggagc cagactggcg
    17941 attgtggatt gttctgccgt gcagcggtgc gctgctgctg tcgcttttcg gcggctggct
    18001 gggtgcgcga ctggttaagg gtaaggcgct gttcaggcag tttgtggggt gatgacaggt
    18061 gttagattgc ctgatgcgct acgcttatca ggcctacgaa gagcgtgcaa tatattgatt
    18121 ttgcaagatt gtgtaggccg gataaggcgt ttacgccgca tctggcatga acagtgtgca
    18181 ttttggcaat aaatatgaaa ccaactgtca tgtcggtttt tttaaaggcc gaaggaggta
    18241 aaccttcggc cttagctacg tttcctcagt tattcaataa actgacggat cagttccagc
    18301 agcgcgatta gcgccttaag aacgatgaga actttatcga gaaaagtttt catccgttgc
    18361 tcccttaagg gagaaagaca atcacagctt acccttacgt tgcctgtaac tatgattttc
    18421 ttcatgtgct agatgaggac tgcctctctc caaattagag caccagaatc agcaccacct
    18481 gtatcctgac caggacttaa aagtactttt ccgcactgcg gtaatccctt gaggcagtat
    18541 cactactagc gtcgggattg tatcgccaac atagctgttt gaatatgtag gcatcctgac
    18601 ttataattca cttgctagat gaggactgtt taatttctgc ccggaacaac caagtcttgg
    18661 gcggaaaaaa taaaaaaacc gactgtcatg tcggtttttt atatctgtat tctggtgcaa
    18721 ccattttgtt ctcacgacaa tcgccgtttc agaataccgc ccatcactgc gccttaaccc
    18781 ataccgccac ttccgcccac tcaccgcgaa agacaacttt ttccgctttt ttctcaagct
    18841 gatagcgata cattgggtcg taatattctt taagtaacgg caccagccag gccagatgac
    18901 cgtcggtgct gccggtggtg agttgcgttg tcagcgccgt atccagctgg gcagcgagtt
    18961 cgttatagcg ctgtaacccc agccgacgct taatcgccga aagtccgtga tgcaggtatt
    19021 cgcaatattc ctgccagccc tgttcgtcgc cataagcgtg ggtaaaatca tgatgcatac
    19081 gcaagaaata ctcttcattc aggcgttcaa gacggatctc aaacggatct tctaccaccg
    19141 caatcgccgc ctgagtcatt cgctcgcgca gacattccgg caggtgattc gaaccgatca
    19201 tccggctttc atcttccagc acccacaggc gcaaatcctg acgggcgtcg gtttttagca
    19261 tttcggcagc cagcaggttt tcaaaactcg cctggctaag ttgtggttgt aacgtgcgac
    19321 caaacgccga accgcgatga cgcgccagcc cttccagatc aacaccgttt ggctgttgtt
    19381 gcactaatag cgttttaccg ctgccggtac aaccgccaat cagcactatc ggtttttgtg
    19441 ccagttcaat ggtcgcctga atcgcggtct ggcgcagtgc cttataaccg ccttccacca
    19501 gcggataatc aatccccgct tcatgcagcc agcgttgcac aatatgtgaa cgttgaccgc
    19561 cacgggcgca gcagagaata ccttgcggat tttgcaggca cgctgcccgc caggcgtaca
    19621 tacgctgctg acgaatttca cccgccacca gtttatgccc cagcgccagc gctgcgtctg
    19681 agccttgctg tttatagcag gtgccaacgg cggcgcgttc atcgttattc attaacggca
    19741 gattgatagc ggcgggcatt gcgccttgct caaactcgat aggggcgcga acatcaataa
    19801 tgggcgtatc agcaatcagc agggcacgat agtcctgttc cgtgtgtctc tcttgcatag
    19861 ttaaaagtga acctcaaatc agcttgcgcg ctattttacg cgccaacgcg caaggaaact
    19921 tgatttttaa ctgcgtgggt tgccgaaaat ttctaaaaat ccgctgattt ccggcctgtg
    19981 ttgggtaaac aaggtcacaa tatcttctac cgctttgccg ctgccaaatt tgcgccatgc
    20041 cagactcaat ggcgaaggag ggcgcatcgt tgggattacc cggctgacca gttgttgatt
    20101 atcgatcatt gactggcaaa gtgattttgg caaaaaacca atgccaacac ccgccagatg
    20161 ggcggcgatt ttcgtttcca tatcgggaac aataatctct ttttgccccg gcaatcgcca
    20221 ggcgacgcgt ttggttaagg tgcgggcgct gtcttcaata ttgaccgccg gaaagcgacg
    20281 caactgcgct tctgttagcg gctcttcaac gttcgccagc ggatgatccg ccgccatgac
    20341 aaatcgccat tgcaccgatc ctaagggatc aagactaaag gtatttgcca gcgcctcagt
    20401 tcccgtgacc ccgatagcca gcgaaaaacc ttcgtacaat agcgagtccc agacgcccat
    20461 atagatttgt cgggagatgt gaaactgggt aaaggggtaa cgctcattca gccacgccag
    20521 caactgggcg acggcctggg ggttatagag caggttgttg atgacaatat tcacctggcg
    20581 ttccacgcca tcattcacct gttgcagctc gctgggcata ctttccagcc agctcagcca
    20641 gtctctggcc tgggaaagta gatgctcgcc agccgctgtc aacgttacgc tacgagtcgt
    20701 acggaaaaac agcgctactc cggtattctc ttccagaagt ttaatgcgat aactgatcgt
    20761 cgccgtggtt ttacataatc gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc
    20821 aatgaaagtc cgcaaggttt ctggatcgaa catcttcagg tatccccttt taaatccgca
    20881 agttgcgtga ttttcttatc ctctgattta tcagtatttt tacatgataa tcctgttcaa
    20941 tttgtggacc aaatctagtt ttggaaaaac attccaactt ttgtattgat gttgttctct
    21001 taaggtttta gattgcctgt tattgaaacc aagctgaccg gtcggcggtg gttgaacgga
    21061 attatgttac aaggacaaaa agatgaaact tcaggtatta ccgttaagtc aggaagcctt
    21121 tagtgcttat ggcgacgtaa tcgaaacgca gcaacgggat tttttccata ttaacaatgg
    21181 cctggtggag cgttaccacg atttggcgct ggttgagatt cttgagcaag accgtacgct
    21241 tatcagcatt aaccgcgcgc aaccggcgaa tctgccgctg accattcacg aactcgaacg
    21301 tcatccgctg ggcactcagg cctttatccc aatgaaaggt gaagtgtttg tggtggtcgt
    21361 ggcgttaggc gacgacaaac cagacctgtc aacgctgcgg gcgtttatca ccaacggtga
    21421 acagggggtg aattaccatc gtaacgtctg gcatcaccca cttttcgcct ggcagcgcgt
    21481 caccgatttt ctgaccatcg atcgcggcgg cagtgacaac tgtgatgttg aaagtattcc
    21541 tgaacaggaa ctctgttttg cgtgacgcct gcaaccgact tgcataagat aaactaattg
    21601 ttcattgttt atgctcactt gtaggtcgga gttaacgtag gtatgacgga agttagacgg
    21661 cgcggcaggc caggacaggc ggagcctgtg gcacagaagg gcgcacaggc gttagagcgg
    21721 ggaattgcga ttctgcaata tctggaaaaa agtgggggaa gttcgtcggt tagcgatatt
    21781 tctctcaatc tggatttgcc gctctccacg acctttcgct tgctgaaggt tttacaagca
    21841 gcggattttg tctatcagga cagtcaatta ggctggtggc atataggatt aggtgtcttt
    21901 aacgtcggtg cggcgtacat ccataaccgc gatgtcctct ccgtcgccgg gccgtttatg
    21961 cgccgcctga tgttactttc cggcgaaacg gtcaatgtcg cgatccgtaa cggcaatgaa
    22021 gcggtattaa ttggtcagtt agagtgtaaa tcgatggtca ggatgtgtgc gccactgggc
    22081 agtcgtctgc cgctgcatgc ttccggtgcg ggcaaagcgc tgctttatcc gctggcggaa
    22141 gaggagttga tgagcatcat tctgcaaacc ggtttgcagc agtttacgcc aactacgctt
    22201 gtggatatgc ccaccttgct gaaggacctg gaacaagcgc gtgaactggg ctataccgta
    22261 gataaagaag agcatgttgt aggtctgaat tgcatagctt cagcaattta cgatgatgtc
    22321 ggtagcgttg ttgccgctat ctccatctcc gggccttcat caagactgac agaagatcgt
    22381 tttgtcagtc agggtgagct ggtcagagac accgcccgcg atatcagcac ggcgttggga
    22441 ctgaaagcac atccatagtg tctgtcgcat cccgctctgc ggagcgggtt tttttgacaa
    22501 aatttaaaag ttggaaaatt tttccaatta atagaggtag gaataaaatg gcaaaaatga
    22561 gagccgttga cgcggcaatg tatgtgctgg agaaagaagg tatcaccacc gccttcggcg
    22621 ttccgggagc tgcaattaat ccgttctact cagcgatgcg taagcacggc ggtattcgtc
    22681 acattctggc gcgtcatgtg gaaggtgttt cgcacatggc ggaaggttat acccgcgcaa
    22741 cggcagggaa tatcggcgta tgtctgggga cttccggtcc tgcgggcacg gacatgatca
    22801 ccgcgctcaa ttccgcttct gctgattcca ttcctattct gtgcattacc ggccaggcac
    22861 cgcgcgcccg tctgcataaa gaagattttc aggccgtaga tattgaagca attgctaaac
    22921 cggtcagcaa aatggcggtt acagttcgtg aagcggcgct ggtgcctcgc gtgctgcaac
    22981 aggcatttca cctgatgcgt tctggtcgtc cgggtccggt actggtggat ttaccgttcg
    23041 acgttcaggt tgcggaaatc gagtttgatc ctgacatgta cgaaccgctg ccggtctaca
    23101 aacctgctgc cagccgtatg cagatcgaaa aagctgtaga aatgttaatc caggccgaac
    23161 gtccggtgat tgttgccggg ggcggggtaa tcaatgctga tgcagctgca ctgttacaac
    23221 agtttgctga actgaccagc gttccggtga tcccaacgct gatgggctgg ggctgtatcc
    23281 cggacgatca tgaactgatg gccgggatgg tcggtctgca aaccgcgcat cgttacggta
    23341 acgcaacgtt gctggcgtcc gacatggtgt ttggtatcgg taaccgtttt gctaaccgtc
    23401 ataccggttc ggtagagaaa tacaccgaag ggcgcaaaat cgttcatatt gatattgagc
    23461 cgacgcaaat tggtcgcgtg ctgtgtccgg atctcggcat tgtctctgat gctaaagcgg
    23521 cgctgacact gctggttgaa gtggcgcagg agatgcaaaa agcgggtcgt ctgccgtgtc
    23581 gtaaagaatg ggtcgccgac tgccagcagc gtaaacgcac tttgctgcgc aaaacccact
    23641 tcgacaacgt gccggtgaaa ccgcagcgcg tgtatgaaga gatgaacaaa gcctttggtc
    23701 gcgatgtttg ttatgtcacc actattggtc tgtcacaaat cgctgcggca caaatgctgc
    23761 atgtctttaa agaccgccac tggatcaact gtggtcaggc tggtccgtta ggctggacga
    23821 ttccggcggc actgggggtt tgtgccgctg atccgaaacg caatgtggtg gcgatttctg
    23881 gcgactttga cttccagttc ctgattgaag agttagccgt tggcgcgcag ttcaacattc
    23941 cgtacatcca tgtgctggtg aataacgcct atctcggcct gattcgtcag tcacaacgcg
    24001 cttttgacat ggactactgc gtgcaactcg ctttcgagaa tatcaactcc agtgaagtga
    24061 atggctacgg tgttgaccac gtaaaagtag cggaaggttt aggttgtaaa gctattcggg
    24121 tcttcaaacc ggaagatatt gcgccagcct ttgaacaggc gaaagcctta atggcgcaat
    24181 atcgggtacc ggtagtagtg gaagttattc tcgagcgtgt gaccaatatt tcgatgggca
    24241 gcgaactgga taacgtcatg gaatttgaag atatcgccga taacgcagcg gatgcaccga
    24301 ctgaaacctg cttcatgcac tatgaataag ggagataaat gatgttacgt ttctctgcta
    24361 atttatcgat gttatttggt gaatatgatt ttctcgcccg ttttgagaaa gctgcgcagt
    24421 gtggttttcg cggcgttgaa tttatgtttc cttatgacta cgacattgaa gaattaaaac
    24481 aggtactggc gagtaataaa ctcgaacata cgctgcacaa tttaccggca ggtgactggg
    24541 cggcgggcga gcgcggtatt gcctgtattc ctggccgtga agaagagttt cgggatggcg
    24601 tagcagcagc gatt
//
LOCUS       DADLTI010000049        24460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLTI010000049 DADLTI010000000
VERSION     DADLTI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.049"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..487)
                     /locus_tag="JN755_001789"
     CDS             complement(<1..487)
                     /locus_tag="JN755_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7380610.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="JN755_001790"
     CDS             760..948
                     /locus_tag="JN755_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380611.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="JN755_001791"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="JN755_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7380612.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="JN755_001792"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="JN755_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="JN755_001793"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="JN755_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7380613.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="JN755_001794"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="JN755_001794"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7380614.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="JN755_001795"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="JN755_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="JN755_001796"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="JN755_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="JN755_001797"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="JN755_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7380615.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /locus_tag="JN755_001798"
     CDS             12308..13594
                     /locus_tag="JN755_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7380616.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="JN755_001799"
     CDS             13815..15311
                     /locus_tag="JN755_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7380617.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16555)
                     /locus_tag="JN755_001800"
     CDS             complement(15407..16555)
                     /locus_tag="JN755_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7380618.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="JN755_001801"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="JN755_001801"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7380619.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="JN755_001802"
     CDS             17405..19465
                     /locus_tag="JN755_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7380620.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="JN755_001803"
     CDS             complement(19647..20969)
                     /locus_tag="JN755_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7380621.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="JN755_001804"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="JN755_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7380622.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23413)
                     /locus_tag="JN755_001805"
     CDS             complement(22442..23413)
                     /locus_tag="JN755_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7380623.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..>24460
                     /locus_tag="JN755_001806"
     CDS             23861..>24460
                     /locus_tag="JN755_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7380624.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5786 a         6159 c         6734 g         5781 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct ggctgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatgttaaa tatctcctcc
//
LOCUS       DADLTI010000050        24188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLTI010000050 DADLTI010000000
VERSION     DADLTI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.050"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(22..594)
                     /locus_tag="JN755_001807"
     CDS             complement(22..594)
                     /locus_tag="JN755_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380625.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JN755_001808"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JN755_001808"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7380626.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN755_001809"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN755_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7380627.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN755_001810"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN755_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7380628.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JN755_001811"
     CDS             complement(3556..5337)
                     /locus_tag="JN755_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7380629.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JN755_001812"
     CDS             complement(5330..7102)
                     /locus_tag="JN755_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7380630.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JN755_001813"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JN755_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN755_001814"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN755_001814"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7380631.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JN755_001815"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JN755_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JN755_001816"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JN755_001816"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7380632.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JN755_001817"
     CDS             complement(11173..11571)
                     /locus_tag="JN755_001817"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7380633.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JN755_001818"
     CDS             complement(11665..12036)
                     /locus_tag="JN755_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380634.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JN755_001819"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JN755_001819"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7380635.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JN755_001820"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JN755_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7380636.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JN755_001821"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JN755_001821"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7380637.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JN755_001822"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JN755_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7380638.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JN755_001823"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JN755_001823"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7380639.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JN755_001824"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JN755_001824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7380640.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JN755_001825"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JN755_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7380641.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JN755_001826"
     CDS             21807..22385
                     /locus_tag="JN755_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7380642.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JN755_001827"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JN755_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7380643.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6661 c         5837 g         6023 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataattgtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 caaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcagtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADLTI010000051        24112 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLTI010000051 DADLTI010000000
VERSION     DADLTI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.051"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1270
                     /gene="glpK"
                     /locus_tag="JN755_001828"
     CDS             <1..1270
                     /gene="glpK"
                     /locus_tag="JN755_001828"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7380644.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1500..2510
                     /gene="glpX"
                     /locus_tag="JN755_001829"
     CDS             1500..2510
                     /gene="glpX"
                     /locus_tag="JN755_001829"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7380645.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            2607..3353
                     /gene="fpr"
                     /locus_tag="JN755_001830"
     CDS             2607..3353
                     /gene="fpr"
                     /locus_tag="JN755_001830"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7380646.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JN755_001831"
     CDS             complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JN755_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7380647.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3813..4112)
                     /locus_tag="JN755_001832"
     CDS             complement(3813..4112)
                     /locus_tag="JN755_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7380648.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4324..4764)
                     /locus_tag="JN755_001833"
                     /pseudo
     CDS             complement(4324..4764)
                     /locus_tag="JN755_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4865..5464
                     /locus_tag="JN755_001834"
     CDS             4865..5464
                     /locus_tag="JN755_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7380649.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5572..6339
                     /gene="tpiA"
                     /locus_tag="JN755_001835"
     CDS             5572..6339
                     /gene="tpiA"
                     /locus_tag="JN755_001835"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7380650.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JN755_001836"
     CDS             complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JN755_001836"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7380651.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7256..8245)
                     /locus_tag="JN755_001837"
     CDS             complement(7256..8245)
                     /locus_tag="JN755_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7380652.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JN755_001838"
     CDS             complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JN755_001838"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7380653.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JN755_001839"
     CDS             complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JN755_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7380654.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JN755_001840"
     CDS             complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JN755_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7380655.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11408..12106
                     /gene="cpxR"
                     /locus_tag="JN755_001841"
     CDS             11408..12106
                     /gene="cpxR"
                     /locus_tag="JN755_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7380656.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12103..13476
                     /gene="cpxA"
                     /locus_tag="JN755_001842"
     CDS             12103..13476
                     /gene="cpxA"
                     /locus_tag="JN755_001842"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7380657.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JN755_001843"
     CDS             complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JN755_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7380658.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JN755_001844"
     CDS             complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JN755_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7380659.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JN755_001845"
     CDS             complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JN755_001845"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7380660.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16553..17587
                     /gene="rhaT"
                     /locus_tag="JN755_001846"
                     /pseudo
     CDS             16553..17587
                     /gene="rhaT"
                     /locus_tag="JN755_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JN755_001847"
     CDS             complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JN755_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7380661.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JN755_001848"
     CDS             complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JN755_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7380662.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19630..21099
                     /gene="rhaB"
                     /locus_tag="JN755_001849"
     CDS             19630..21099
                     /gene="rhaB"
                     /locus_tag="JN755_001849"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7380663.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21096..22355
                     /gene="rhaA"
                     /locus_tag="JN755_001850"
     CDS             21096..22355
                     /gene="rhaA"
                     /locus_tag="JN755_001850"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7380664.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22597..23421
                     /gene="rhaD"
                     /locus_tag="JN755_001851"
     CDS             22597..23421
                     /gene="rhaD"
                     /locus_tag="JN755_001851"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7380665.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23431..23745
                     /gene="rhaM"
                     /locus_tag="JN755_001852"
     CDS             23431..23745
                     /gene="rhaM"
                     /locus_tag="JN755_001852"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7380666.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24046..>24112
                     /locus_tag="JN755_001853"
     CDS             24046..>24112
                     /locus_tag="JN755_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY7380667.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         5982 a         6297 c         6132 g         5701 t
ORIGIN      
        1 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
       61 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
      121 tttagaagat tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac
      181 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
      241 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
      301 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
      361 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
      421 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
      481 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
      541 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
      601 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
      661 ctatgcgttg gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga
      721 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
      781 caatggtgtg tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta
      841 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
      901 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
      961 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     1021 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
     1081 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
     1141 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     1201 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     1261 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     1321 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     1381 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
     1441 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
     1501 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     1561 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     1621 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     1681 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
     1741 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
     1801 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
     1861 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     1921 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     1981 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     2041 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     2101 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
     2161 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     2221 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     2281 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     2341 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
     2401 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
     2461 tccactatct ggatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat
     2521 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
     2581 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
     2641 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
     2701 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
     2761 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     2821 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
     2881 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
     2941 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
     3001 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
     3061 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
     3121 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
     3181 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
     3241 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
     3301 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
     3361 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
     3421 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
     3481 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     3541 tgtcttcgga cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc
     3601 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     3661 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     3721 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     3781 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     3841 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
     3901 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     3961 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
     4021 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     4081 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     4141 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     4201 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
     4261 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     4321 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     4381 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     4441 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
     4501 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     4561 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     4621 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
     4681 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     4741 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     4801 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     4861 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     4921 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     4981 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     5041 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     5101 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     5161 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
     5221 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
     5281 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     5341 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     5401 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     5461 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     5521 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     5581 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     5641 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     5701 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
     5761 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
     5821 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
     5881 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
     5941 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
     6001 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     6061 atcgcttatg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     6121 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     6181 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
     6241 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
     6301 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     6361 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
     6421 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
     6481 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
     6541 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
     6601 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     6661 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
     6721 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     6781 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
     6841 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
     6901 acgatacgtt gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
     6961 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
     7021 caaacattcc tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
     7081 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
     7141 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
     7201 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
     7261 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
     7321 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
     7381 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
     7441 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
     7501 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
     7561 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
     7621 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
     7681 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
     7741 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
     7801 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
     7861 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
     7921 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
     7981 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
     8041 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
     8101 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
     8161 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
     8221 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
     8281 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
     8341 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
     8401 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
     8461 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
     8521 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
     8581 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
     8641 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
     8701 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
     8761 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
     8821 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
     8881 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
     8941 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
     9001 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
     9061 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
     9121 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
     9181 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
     9241 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
     9301 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
     9361 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
     9421 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
     9481 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
     9541 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
     9601 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
     9661 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
     9721 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
     9781 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
     9841 aaaggcagag agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc
     9901 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
     9961 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    10021 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    10081 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    10141 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    10201 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    10261 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    10321 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    10381 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    10441 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    10501 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    10561 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    10621 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    10681 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    10741 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    10801 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    10861 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    10921 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    10981 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    11041 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    11101 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    11161 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    11221 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    11281 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    11341 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    11401 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    11461 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    11521 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    11581 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    11641 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    11701 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    11761 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    11821 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    11881 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    11941 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    12001 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    12061 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    12121 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    12181 ttacccaagc tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg
    12241 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    12301 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    12361 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    12421 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    12481 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    12541 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    12601 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    12661 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    12721 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    12781 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    12841 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    12901 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    12961 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    13021 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    13081 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    13141 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    13201 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    13261 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    13321 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc
    13381 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    13441 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    13501 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    13561 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    13621 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    13681 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    13741 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    13801 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    13861 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    13921 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    13981 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    14041 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    14101 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    14161 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    14221 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    14281 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    14341 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    14401 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    14461 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    14521 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    14581 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    14641 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac
    14701 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    14761 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    14821 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    14881 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    14941 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    15001 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    15061 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    15121 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    15181 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    15241 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    15301 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    15361 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    15421 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    15481 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat
    15541 tgtttttatt ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    15601 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    15661 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc
    15721 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    15781 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    15841 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    15901 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    15961 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    16021 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    16081 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    16141 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    16201 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    16261 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    16321 ggtttattat agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa
    16381 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    16441 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    16501 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    16561 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    16621 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    16681 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    16741 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    16801 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    16861 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    16921 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt
    16981 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    17041 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    17101 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    17161 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    17221 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    17281 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    17341 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat
    17401 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    17461 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    17521 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    17581 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    17641 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    17701 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag
    17761 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    17821 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    17881 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    17941 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    18001 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    18061 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    18121 aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    18181 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    18241 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    18301 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    18361 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    18421 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    18481 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    18541 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    18601 atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg
    18661 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    18721 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    18781 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    18841 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    18901 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    18961 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    19021 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    19081 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    19141 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    19201 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    19261 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat
    19321 ccacactacg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    19381 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    19441 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag
    19501 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    19561 agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    19621 gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc
    19681 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    19741 tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa
    19801 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    19861 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    19921 ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa
    19981 ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt
    20041 tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    20101 ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc
    20161 aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    20221 gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    20281 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    20341 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    20401 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    20461 gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    20521 atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg
    20581 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    20641 cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    20701 caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    20761 ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg
    20821 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc
    20881 ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag
    20941 ttaattacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    21001 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    21061 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    21121 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    21181 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    21241 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    21301 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    21361 cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    21421 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    21481 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    21541 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    21601 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    21661 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    21721 atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    21781 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    21841 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga
    21901 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    21961 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    22021 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    22081 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    22141 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    22201 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    22261 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    22321 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc
    22381 cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc
    22441 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    22501 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    22561 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    22621 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    22681 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    22741 ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg
    22801 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct
    22861 aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta
    22921 accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc
    22981 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    23041 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    23101 agcaccgaat gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    23161 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    23221 tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc
    23281 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    23341 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    23401 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta
    23461 aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa
    23521 gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat
    23581 ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    23641 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac
    23701 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    23761 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    23821 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    23881 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    23941 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    24001 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    24061 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DADLTI010000052        23922 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLTI010000052 DADLTI010000000
VERSION     DADLTI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.052"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            10..333
                     /locus_tag="JN755_001854"
     CDS             10..333
                     /locus_tag="JN755_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7380668.1"
                     /translation="MPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDA
                     GVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNEL
                     EHQTK"
     gene            386..2116
                     /locus_tag="JN755_001855"
     CDS             386..2116
                     /locus_tag="JN755_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380669.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2176..2667)
                     /gene="yajQ"
                     /locus_tag="JN755_001856"
     CDS             complement(2176..2667)
                     /gene="yajQ"
                     /locus_tag="JN755_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7380670.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2835..3746
                     /gene="panE"
                     /locus_tag="JN755_001857"
     CDS             2835..3746
                     /gene="panE"
                     /locus_tag="JN755_001857"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7380671.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3709..4299
                     /gene="yajL"
                     /locus_tag="JN755_001858"
     CDS             3709..4299
                     /gene="yajL"
                     /locus_tag="JN755_001858"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7380672.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4353..5801)
                     /gene="thiI"
                     /locus_tag="JN755_001859"
     CDS             complement(4353..5801)
                     /gene="thiI"
                     /locus_tag="JN755_001859"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7380673.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6007..6249
                     /gene="xseB"
                     /locus_tag="JN755_001860"
     CDS             6007..6249
                     /gene="xseB"
                     /locus_tag="JN755_001860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7380674.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6249..7148
                     /gene="ispA"
                     /locus_tag="JN755_001861"
     CDS             6249..7148
                     /gene="ispA"
                     /locus_tag="JN755_001861"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7380675.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7173..9035
                     /gene="dxs"
                     /locus_tag="JN755_001862"
     CDS             7173..9035
                     /gene="dxs"
                     /locus_tag="JN755_001862"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7380676.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9090..10064
                     /gene="yajO"
                     /locus_tag="JN755_001863"
     CDS             9090..10064
                     /gene="yajO"
                     /locus_tag="JN755_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7380677.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10118..10636)
                     /gene="pgpA"
                     /locus_tag="JN755_001864"
     CDS             complement(10118..10636)
                     /gene="pgpA"
                     /locus_tag="JN755_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7380678.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10614..11591)
                     /gene="thiL"
                     /locus_tag="JN755_001865"
     CDS             complement(10614..11591)
                     /gene="thiL"
                     /locus_tag="JN755_001865"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7380679.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11669..12088)
                     /gene="nusB"
                     /locus_tag="JN755_001866"
     CDS             complement(11669..12088)
                     /gene="nusB"
                     /locus_tag="JN755_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7380680.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12108..12578)
                     /gene="ribE"
                     /locus_tag="JN755_001867"
     CDS             complement(12108..12578)
                     /gene="ribE"
                     /locus_tag="JN755_001867"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7380681.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="JN755_001868"
     CDS             complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="JN755_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7380682.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13774..14223)
                     /gene="nrdR"
                     /locus_tag="JN755_001869"
     CDS             complement(13774..14223)
                     /gene="nrdR"
                     /locus_tag="JN755_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7380683.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14374..14913
                     /locus_tag="JN755_001870"
     CDS             14374..14913
                     /locus_tag="JN755_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7380684.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15212..16096
                     /locus_tag="JN755_001871"
     CDS             15212..16096
                     /locus_tag="JN755_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7380685.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16273..16620)
                     /locus_tag="JN755_001872"
     CDS             complement(16273..16620)
                     /locus_tag="JN755_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7380686.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16749..17720)
                     /gene="secF"
                     /locus_tag="JN755_001873"
     CDS             complement(16749..17720)
                     /gene="secF"
                     /locus_tag="JN755_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7380687.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17731..19578)
                     /gene="secD"
                     /locus_tag="JN755_001874"
     CDS             complement(17731..19578)
                     /gene="secD"
                     /locus_tag="JN755_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7380688.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19606..19938)
                     /gene="yajC"
                     /locus_tag="JN755_001875"
     CDS             complement(19606..19938)
                     /gene="yajC"
                     /locus_tag="JN755_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7380689.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19961..21088)
                     /gene="tgt"
                     /locus_tag="JN755_001876"
     CDS             complement(19961..21088)
                     /gene="tgt"
                     /locus_tag="JN755_001876"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7380690.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21143..22213)
                     /gene="queA"
                     /locus_tag="JN755_001877"
     CDS             complement(21143..22213)
                     /gene="queA"
                     /locus_tag="JN755_001877"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7380691.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22306..22879
                     /gene="acpH"
                     /locus_tag="JN755_001878"
                     /pseudo
     CDS             22306..22879
                     /gene="acpH"
                     /locus_tag="JN755_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22884..>23922)
                     /gene="malZ"
                     /locus_tag="JN755_001879"
     CDS             complement(22884..>23922)
                     /gene="malZ"
                     /locus_tag="JN755_001879"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7380692.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6078 a         6270 c         5880 g         5694 t
ORIGIN      
        1 ggcaatggta tacctgcaaa caatgaaaaa tacctagcat atctgcaaca agctgcaaca
       61 ctgggcagta gtaatgccca ggcggaaatt ggctatttat atctcatcgg aaaagaacta
      121 ccacagaatc ttcctgatgc tggtgtctgg ttcaaaaaag cagctgcaca aggtaatttt
      181 gccgcacatt tctatctggg aaggatgtat cagaatggtg acggcgttga gagaaatatg
      241 gaaaaagcca ggtttcatct atctaatgcg gcagaaggtg gaatagaacc tgcgcttaaa
      301 gcattaaatg aacttgaaca tcaaacaaaa taaaacgctc agggaccacg taaggttcat
      361 caaacagaaa aggatatagt ttgcgatgaa agcgaaatct actttgttat taatgatttt
      421 atctgttttt tattctcatc aggttttatc cagcgacccc actcagccgg atgtttattc
      481 tgtcgtcgag aaaaaactaa actctgcttt acctctggaa gaaaatacca ccttcagatc
      541 gcaggctgaa tggtttaatt tgcaatatga attattagta tctggctacc ctgaacgtgc
      601 ttacaattta ctgagcaaac ttgagacaga atcgaaaaac gctaaatatt atctggattt
      661 aactgcctcc agagtcgctg aagaagaagc accagagaca gccttaactt ttttatcaaa
      721 aattgggtta aataaaccgg ctactcttac atcctttgag agctatatca atagctggat
      781 aaaaagcaaa caacccgaaa aggctatgca gcttctcagc ctcgatgaga atgccctgaa
      841 ttattattta ccgactgtgt tagtcgcata tcagatcgtt cctgataagg caataagtat
      901 ttataattct gtatatggag ataatgttgt tataccctat cagcaagtca ccatgttact
      961 tacagttgcg gagggttatc aaacaaaagg cgatataaaa aacgccattc attatgccga
     1021 taaggcgctg aatatgtttg ataaagctat tgctgtagat agcagtcgtg aatcccatta
     1081 tagtgatgag tatttgaaac tcatgaatat ctacgccgcc aatggcaaca aagaaaaagc
     1141 ggtggtttta tctcaacgtc tacacaaagc cattaccgaa tctcaatcga attacttaag
     1201 tactctggaa ggaattttat tgttttatag aaataatgat atgcagcaag actatcaaaa
     1261 cagcctttca aattacatag tatttatcga tgaaattttt gccttttccc catcaacaag
     1321 aagtgaatta agcctcatta atttattaaa ttcattaaat gaggtggaat tgatgagaaa
     1381 acgtctggcg tttctgatga cagcaccaga atacgcttgt tatgatagtc agtattgtta
     1441 tgaagacaaa gtcagcgcat taaaaatcct gtatcgtcat catgacaatg agctaatgga
     1501 caaatatttc acgactcttc ttaaagagat cagtaatcaa ccattagaag actgggatat
     1561 aataataagt aatactggtg aaaaatttag tgaagtgggt ctaaccaaat atgctcaaca
     1621 tctcgcatct ttcgctgaag aattttatat aaaagagcaa aaatcacatt ctgaaagtga
     1681 aatgagagga ctattttcat cacttgccga attatatagt tttggtaatg atactgccag
     1741 cgcaaaccga gtgttccatc agcatgtccc tgccttcaat acagatcata tgattcaata
     1801 ttttataaat gccaaagaat ggaacaatgc cagggaatta ttgataaaag aggaaatgtt
     1861 gggtatgcat aatctgatat tgttggagaa tatttgtatg caaaaaaatg cagaatgcat
     1921 ggcacacatt acctttacgc ttaataaatt aacaacacaa ccagcaataa caaaaataga
     1981 tagcgtgggt aatgagcaac tttatcagat tggcaaaatc taccataagt tggaaattaa
     2041 acctgagcca gagcaacagg ttttaattca gtcgctttat gaccgggcat caggaagcgc
     2101 ctctgcaact caataaatgc atggtcaaaa gggccaccac ggtggccctg acaatcagta
     2161 gctatatcca gaaagttaat cgcggaagtt tttgaactgg aacggctgac cgagatcgcc
     2221 accgcgcacc atcgccatga cagcctgcaa atcatcacga gatttgccag tcacgcggat
     2281 ctcatcgccc tgaatttgcg cctgcacttt cagtttgctg tctttgatca tcttgacgat
     2341 ttttttctga gtggcgctct caatgccctg tttcagtttc gcttccacaa accaggtttt
     2401 accgctatga acgatatttt ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag
     2461 cagcttggca cgcagaatat ccagcaactg attgacctgg aagtcggact cgctcaacac
     2521 tttgatggtt ttgctggcgt cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa
     2581 acgggactcc acttcgcgac tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac
     2641 ttcagagaca atatcgaaag atggcatctt ttcctctccc ttcatttttg atgcgaagca
     2701 taatacctgc aaagttaaat aaccaacagt cctgcaccag gacgtatccc ttaattgtgc
     2761 atagtcgcaa cagcatgcac aattaaggga tagccttatt gaacacccgg agtggttgtg
     2821 ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg tgccttaggg caattatggc
     2881 ttacagcact ttgtaaacag ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt
     2941 attgtagtgt gaatctggtt gagacagatg gttcgatatt taacgaatcg ctgaccgcca
     3001 acgatcccga ttttctcgcc accagcgatc tgctcctggt gacgctgaaa gcatggcagg
     3061 tttccgatgc cgtcaaaagc ctcgcatcca cactgcctgt aactacgcca atactgttaa
     3121 ttcacaacgg catgggcacc atcgaagaat tgcaaaacat tcagcagcca ttactgatgg
     3181 gcaccaccac ccatgccgcc cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa
     3241 tcacgcatat cggtccggca cggcaacagg atggcgatta cagttatctg gcggatattt
     3301 tgcaaaccgt gttgcctgac gttgcctggc ataacaatat tcgcgccgag ctgtggcgca
     3361 agctggccgt caactgcgtg attaatccac tgactgccat ctggaattgc ccgaacggtg
     3421 aattacgtca tcatccgcaa gaaattatgc aaatatgcga agaagtcgcg gcagtgatcg
     3481 aacgcgaagg gcatcatact tcagcggaag atttgcgtga ttacgtgatg caggtgattg
     3541 atgccacagc ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg
     3601 aaatcgacta tatcaatggt tttctcttac gccgcgcccg cgcgcatggg attgccgtac
     3661 cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg
     3721 gcactggttt gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac gatcgacctc
     3781 ttggttcgcg gcggtatcaa agtcaccact gccagcgtcg acagcgatgg caacctggca
     3841 attacctgct cgcgcggcgt gaagctgctg gcggatgcgc cgctggtcga agtggctgat
     3901 ggcgaatatg acgtgatcgt gctgcctggt ggcattaaag gcacggagtg ttttcgcgat
     3961 agcactctgc tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct
     4021 atttgcgccg cgccagccac cgtgctggtg ccgcacgata tctttccgat tggcaatatg
     4081 accggcttcc cgacgctgaa agacaaaatt cccgctgaac aatggcagga caagcgcgtc
     4141 gtctgggatg cacgggtaaa attgctgacc agccaggggc cgggtacagc tatcgacttt
     4201 ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag cccatgaagt ggcatcacaa
     4261 ctggtgatgg cggcagggat ttataattat tacgagtagt gtcggatgcg gcaaacgtcg
     4321 catctgacca gatgcgacgt aaaaactaca aattacgggc gatatacctt cacattgtta
     4381 aagccttgct cgcgcagata gagcgcctgc agacggctca tcaccccgcg ctcacaccac
     4441 agcagccagg ttttgttctg gtcgagatcg ccaaatttgg tgctcagttt atagaacggc
     4501 agagaaacca catcaatccc ttcgactttc agtggcttat cttcctgttc atcgacagaa
     4561 cggatatcga gaatcacgtc gttcgggccg aagccgttga cggtttccac ttccaccact
     4621 tcctgctcgg tctgctgggc gatttcgcgg atatcaacgt tattcgcttc ctcaaccact
     4681 ttatcgagaa tactgaagtc gaacttctct tcttccgctt caatcttcga tttaaccgct
     4741 ttcaccgtcg ggcttttgga gatcacgcca caatattccg gcatcgtgcg agcaaagtct
     4801 tcggtgccaa tctggcgagc caggttgatg atgtgctctt tgtcgtaaga aatcagcgga
     4861 cgcaggatca gcgtgtcaga gacgttatca atcaggcgca ggttggtcag cgtctgactg
     4921 gacacctggc cgagcgcttc gccggtgacc agcgcctgta cgccgtaacg ttcagccact
     4981 ttagatgcgg cacgcaccat catacgtttg aggataacgc ccatctgacc gtcgtcgatt
     5041 ttctcgagaa tttccccgac aaccggttcg aaattaatgg cgacaaaacg cacgcggtgg
     5101 gagctgccaa agcggttcca cagataatgc gccacctgac gcacgccaat ttcatgcgct
     5161 gcgccgccga ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat caacatataa
     5221 ctggaaacac cggagtcgaa accaccggaa atgagcgaca gcacatcttc ctgggtgcca
     5281 atcgggaaac caccaatacc ttcgtagcga cctttaatca gcaggaggcg atcgtcttcc
     5341 acttccagat gaaccgttac atccgggtta gtcagcttca cgcgcgcgga ttcaatatgc
     5401 tgatttaaac cgccgccgac gtaacgttcc acatcaatcg agctaaaatc atgtttgcca
     5461 cggcgcttca cgcgtacgca gaaggttttg ccttccagct gatcgcgata ctgaaccaac
     5521 gctttctcaa aaatatcgtg catgtcggta aacggcacgt cttcgacttc gagaatatgg
     5581 tggatacccg gaatacgggt cagggcgtcg cgaatagtca gacgctggtt ttcatctttc
     5641 gcgcgaactt cgatgttatc ccagtggcgg acgacagcga gcgtctcatc atagtgcttt
     5701 aaaacgttac gaatgttccc ggtaaggatt tttataaagc gcaagcgcac agattggctt
     5761 ttgatggtga tttccgggaa caatttaatg ataaacttca tggcggcaat ggttcgttgg
     5821 caagccttaa gcgacttgta tagggaaaaa tacagcagcc cacacctgcg gctgcatcca
     5881 ggcgcggaag tataccacta acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt
     5941 atttgctatt tgccctgagt ccgttaccat gacggggcga aaaatattga gagtcagaca
     6001 ttcattatgc cgaagaaaaa tgaggcgccc gccagctttg aaaaggcgct gagcgagctg
     6061 gaacagattg taacccgtct ggaaagtggc gacctgccgc tggaagaggc gctgaacgag
     6121 ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca aattacaaca agccgaacag
     6181 cgcgtacaaa ttctgctgtc tgacaatgaa gacgcctctc taaccccttt tacaccggac
     6241 aatgagtaat ggactttccg cagcaattcg aagcctgcgt taagcaggcc aaccaggcgc
     6301 tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt
     6361 atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc accggtcata
     6421 tgtttggcgt tagcacaaac acgctggacg cacccgctgc tgccgtagag tgtatccacg
     6481 cttactcatt aattcatgat gatttaccgg cgatggatga tgacgatctg cgtcgcggtt
     6541 tgccgacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggc gacgctttac
     6601 aaacgctggc gttctcgatt ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca
     6661 gaatttcgat gatttctgaa ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc
     6721 aggcattaga tttagacgcg gaaggcaaac acgtacctct ggacgcgctt gagcgtattc
     6781 atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg
     6841 gagataaagg gcgtcgtgct ctgccagtac tcgacaagta cgcagagagc atcggccttg
     6901 ccttccaggt tcaggatgac atcctggatg tggtaggaga tactgcaacg ttgggaaaac
     6961 gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc
     7021 aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactgg
     7081 ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta
     7141 ataaataaac aataagtatt aataggcccc tgatgagttt tgatattgcc aaatacccga
     7201 ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga
     7261 aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact
     7321 tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaacaccc
     7381 catttgacca attgatttgg gatgtgggac atcaggctta tccgcataaa attttgaccg
     7441 gacgccgcga caaaatcggc accatccgtc agaaaggcgg tctgcacccg ttcccgtggc
     7501 gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg
     7561 gaattggtat tgcggttgct gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtga
     7621 ttggcgacgg cgcgattacc gctggcatgg cgtttgaagc gatgaatcac gcgggcgata
     7681 ttcgtcctga tatgctggtg gtcctcaacg acaatgaaat gtcgatttcc gaaaatgttg
     7741 gcgcgcttaa caaccatctg gctcagctgc tttccggtaa gctttactct tcactgcgcg
     7801 aaggcgggaa aaaagttttc tctggcgtgc cgcccattaa agagctgctc aaacgtaccg
     7861 aagaacatat taaaggcatg gtggtgcctg gcacgttgtt tgaagagctg ggatttaact
     7921 acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgcta aagaacatgc
     7981 gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac
     8041 cggcagaaaa agacccgatc actttccacg ccgtgcctaa atttgatccc tccagcggtt
     8101 gtttgccgaa aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt
     8161 gcgaaacggc agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt
     8221 ccggcatggt cgagttttca cgtaaattcc cggatcgtta cttcgacgtg gctattgccg
     8281 agcaacacgc agtgaccttt gccgccggtc tggcgattgg tggatacaaa cccattgtcg
     8341 cgatctactc caccttcctg caacgcgcct atgatcaggt gctgcatgac gtggcaattc
     8401 aaaagctacc ggtcctgttc gccattgacc gcgcgggcat tgttggtgct gacggtcaaa
     8461 ctcaccaggg cgcttttgat ctctcttacc tgcgctgcat accagaaatg gtcattatga
     8521 ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg
     8581 gcccgtccgc ggtgcgctac ccgcgcggca acgcggttgg cgtggaactg acgccgctgg
     8641 aaaaactacc aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gtgatcctta
     8701 actttggtac gctgatgcca gaagcggcca aagtcgccga atcgctgaac gctacgctgg
     8761 tcgatatgcg ttttgtgaaa ccacttgatg aagcgttaat tctggaaatg gccgccagcc
     8821 atgaagcgct ggtcaccgta gaagaaaacg ccattatggg cggcgcaggt agcggagtga
     8881 acgaagtgct gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact
     8941 tctttattcc gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgccg
     9001 gtatggaagc caaaatcaag gcctggctgg cataatccct actccactcc tgctatgctt
     9061 aagaaattat tcatggcagg agtggcagca tgcaatacaa ccccttagga aaaaccgacc
     9121 ttcgcgtttc ccgactttgc ctcggctgta tgacctttgg cgagccagat cgcggtaatc
     9181 acgcatggac actgccggaa gagaacagcc gtcccataat taaacgcgcg ctggaaggtg
     9241 gcataaattt ctttgatacc gccaatagct attccgatgg tagcagcgaa gagatcgttg
     9301 gtcgcgcact gcgggatttc gcccgtcgtg aagacgtagt cgttgccacc aaagtgttcc
     9361 atcgcgttgg tgatttaccg gaaggattat cccgtgcaca aattttgcgc tctatcaacg
     9421 acagcctgcg acgtctcggc atggattatg tcgatatcct gcaaattcat cgcggggatt
     9481 acaacacgcc gatcgaagag acgctggaag ccctgaacga cgtggtaaaa gccgggaaag
     9541 cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt tgctcaggcg ctggaactac
     9601 aaaaacagca ctgctgggcg cagtttgtca gtatgcagga tcactataat ctgatttatc
     9661 gcgaagaaga gcgcgagatg ctgcctctgt gttatcagga gggcgtggcg gtgattccgt
     9721 ggagcccgat ggcgcggggg cgactgacgc gtccatgggg agaaactacc gcacgactgg
     9781 tgtctgatga ggtggggaaa aatctctata aagaaagcga tgaaaatgac gcgcagatcg
     9841 cagagcggtt aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg
     9901 cctggttgtt aagtaaaccg ggcattgccg caccgattat cggaacttcg cgggaagaac
     9961 agcttgatga gctgctgaac gcggtggata tcactttgaa gccagaacag attgccgagc
    10021 tggaaacgcc gtataaaccg catgcggtcg taggatttaa ataagagtcc aggcctgatg
    10081 agacgtgaca agcgtcacat caggcatcgg tgcacaacta cgacagaata cccagcggcc
    10141 agtgatgacc gataaagtac aggatgcctg cggaaatcac cccggcgaca atatcgtcga
    10201 tcatgatccc catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc
    10261 acatatccag aatacggaaa atcacaaacc cggcggcaac ccactgccag tcattggtcg
    10321 gcagcgccat gagcgtgatc cacataccaa taaattcgtc ccagacaatg ctgccgtgat
    10381 cgtgcacgcc catatctttc gccgtttgat gacaaagata gacgccgata cagatcccca
    10441 acatcaccac cagcgagtag agctgccagg gtaaaaaggt catcagatac cagaaaggga
    10501 tcgccgccag cgatcccatc gtgccaggaa cgattgggct taatccactt ccgaatccga
    10561 cagcaagcag atgccacgga ttactcatct tcaggcgact tttcgcgaca tctttatggc
    10621 gtggcaaaat ggtcatatcc tttccagtca aacgtgacag gttcgccgtc acgaataaaa
    10681 caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac gccaaggtgt
    10741 ccgagagcaa catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa
    10801 tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc
    10861 gcatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg
    10921 atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg
    10981 cgcagtgcct gcccctgtaa aatacgcggc gatggacgga gatgacgttt gatcaagtag
    11041 tcagcatctt tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcggcg
    11101 ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt
    11161 aaggctcgtc ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc
    11221 gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa caaactgtcg
    11281 ctgaaggact caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag
    11341 gccggatcgg cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc
    11401 agatcagcag gatcgatatc agggaggaaa tggttgcccg ccaccagcgt atcagtgctg
    11461 atcgccaggg tctgtttctc ggggatattg agaagagcgc aatcgtcgcc gatgcccagt
    11521 tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg
    11581 ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcatggaaca
    11641 gtcttccgtg aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg
    11701 ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca
    11761 gttcgatcgc ttcgttaatg gccactttgt atggcacatc actacgttta gacagttcgt
    11821 acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc aggcgggaca
    11881 ggtagggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg gccagcagct
    11941 cacggaagta cagaacgtca acgtctttta catcctgttc agccaggaac tggtattcaa
    12001 catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg gcacactcac
    12061 gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg
    12121 gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca
    12181 gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa
    12241 gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg
    12301 acatattcaa agtgggcaga gccaccacga ataaccgtac ccagcgcaat caccgcgtcg
    12361 tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc accaggcacc
    12421 caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc gtcaattgca
    12481 ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga
    12541 gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta
    12601 tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc
    12661 gggagatcat gcccccacta aatgcaggca aacatccggg cctacatgac gtatctcttt
    12721 gaatttaaat tggggggcgt cggctaattt ctcaagccct ggcagcgagc ataatccacg
    12781 ggcgtcgctg cctaatagtt taggtgcgat atagacaatc agttcatcga ctaaacccgc
    12841 ctgtagcaat acgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg
    12901 tttacccagt tgcatcatca gtacaaccag atccagatga cctttatgct ctggaatcag
    12961 caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt
    13021 ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc tatcaatcac
    13081 aatacgtact ggctggcgga gattttgttg cgggtagagc gcctgagttt gttcatccag
    13141 ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc tgctggttaa
    13201 aatggcatga ctttgtgcgc gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat
    13261 ccactggctt tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc caagttttaa
    13321 ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcggc
    13381 ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc
    13441 acgcccagcg acctgcgggt taggatcttg catcgctgcg accacgcgcg ctacgccagc
    13501 ggcgatgagt gcgtcacagc acggtggcgt acggccatga tggctacagg gttcgagtgt
    13561 cacatacgcc gttgcgcctt tggctttttc acccgccata cgcaacgcgt gtacttcggc
    13621 atgtggttca cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat ctttgacaat
    13681 gacgcacccg acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag
    13741 cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc
    13801 gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac
    13861 tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc tcggtacttc
    13921 gcgctcaccg gtggcgcgca gctgcgattt aatatgattg attgccattt caacgtaatc
    13981 ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgcaatt tctcttcatt
    14041 aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc
    14101 aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc
    14161 ctcgcccacg agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg
    14221 catacggcgt cctgaccagg tggttaacgg aaatctattt taccctgaac tgacgataca
    14281 acaaaggcgc aacgcttttg agataaagga tcgatgcttt cgctgctttt accctctacc
    14341 attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc
    14401 ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg
    14461 aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa
    14521 atcactcagc aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga
    14581 gcgaaaacac cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat
    14641 attacgcctg atacagatgg caccaccctt acactacgta ttcagggtga gtcgaatgat
    14701 cccttgcccg cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac
    14761 tttcaggaaa tcaatgtgca gaatcaattg attaatgccc ctgccagcgt cctcgccccc
    14821 agcgatgttg atattccgtt acaattaaag gggatttctg tggatcagtt aggctttgta
    14881 cgtatccacg acattcaacc tgttatgcag taaacgtatt ttgggacgat tttgtgcgtc
    14941 ccgcaacatc tttccccgtc attttgttac tctgcttaca tcacttggat tgatagtaaa
    15001 agtttgcaac aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg
    15061 caatcgatta cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt
    15121 ttataatagg ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc
    15181 ccgcaaggga ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc
    15241 ggtactggcg ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta
    15301 tctttccgac tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg
    15361 accgcagatc cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt
    15421 cgacttctat ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgctaaagg
    15481 tatctggaac cacggttctc cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa
    15541 gctgaccaat actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta
    15601 catttacgac atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg
    15661 taccgatatc gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca
    15721 gtggcagaac tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa
    15781 atactttgtg ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa
    15841 cttcgactgg ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac
    15901 ccgtactaat aactctatcg cttccagcca tattctggct ctgaactacg atcactggca
    15961 ctactctgtc gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact
    16021 gaacttcggc aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt
    16081 aggttacaac ttctgattat gaaaatgccg ggatttattc ccggcatttc tgattgttaa
    16141 caacatgtgc tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca
    16201 cgtaaattca atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat
    16261 tttacgtttt aatcacttct tcttgttcat catcgctttc agatcagcga atgggttgta
    16321 cttcgcttca ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg
    16381 atccgcttcg gtatatttcg aatgctcatg atcgtggcaa tagagacaca acaattccca
    16441 gttactgcca tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc
    16501 acgcaggtta gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt
    16561 taatgctttt tcgcgatagc cgctttctaa ccgtgcatag ttttttggga tgatagccat
    16621 gaaaagttac ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc
    16681 gcaatctcca tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcaacgg
    16741 gaacttgatt aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc
    16801 aacatgtgtt cgcgcttcat acccagtttc agagccaacg cagatgctac gtagatggaa
    16861 gatgcagtgc cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga
    16921 ccaccgaaga gatacagcat caggataacc atcaaggtag taccggatgt gatcaaggta
    16981 cggtgcagcg tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc
    17041 ttgcggaagt tttcacgaat tcggtccgat accacgatac tgtcgttaag cgagtaaccg
    17101 ataaccgaca tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa
    17161 atacccagcg taatgataac gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc
    17221 cactcaaagc ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc
    17281 gcaccggttt gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact
    17341 gctgcattct gattggtgga ttcgttaatc accttcagaa cttggctgcc cagcacctga
    17401 ccgccggttt cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag
    17461 ttttgcagca ttggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt
    17521 tcagccggtt tttcgagcgt aatttcgata accgtaccac cggtgaaatc cagcccccag
    17581 ttaaagccgc gcacgcccat aataacgata gcagcgatta acagcagacc agagatgccg
    17641 aaagcccagt agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca
    17701 acagtatatt cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc
    17761 gccatatagc aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc
    17821 cacaccgata ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag
    17881 gatgatgact ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg
    17941 ataaccttca tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg
    18001 ttcgttgatc agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat
    18061 acctggcata ctcagtgttg cgcctggcaa cagcgacata ataccgacga tcaagatcaa
    18121 gttggcaatc agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa
    18181 cagaatagaa accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat
    18241 acccagggtc gggccaattg tacgttcttc aacaatctga atcggcgcga tcaacgcacc
    18301 ggcacgcagc agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg
    18361 gaagctgtta cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac
    18421 cagaaccgca cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt
    18481 tgccatcggt ttgccgatgt tgtccttggt gaagttagac ataatgttgc caccagcgct
    18541 atcgagcgag atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt
    18601 gatatggtca ccagtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt
    18661 ctgtttcact tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacatt
    18721 ggtgtttacc agacggaatt ccagcgttgc cgtcgcgccc agaatctctt tcgcacgcgc
    18781 agtgtcctga ataccaggca gttcaacaac gatacggtca gcaccctgac gctgaaccac
    18841 cggctccgcc acgccaagtt ggtttacacg gttacgcagg atattaatgt tctgctgcac
    18901 cgcatattca cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct
    18961 gccctggctg ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc
    19021 acgagcttta gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacagt
    19081 ggtatacggg atgccctttt cgcgcaggtc actgcgtagg ctatcgatat tttgttcctg
    19141 gagtttgcca agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag
    19201 gtcaaggccg agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg
    19261 ggcaaggtta agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc
    19321 gcgcaactgg gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac
    19381 agacttagca gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc
    19441 actggcggcg actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt
    19501 gggaagcgca tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa
    19561 aggataacgg tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat
    19621 ggtgcctttc ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc
    19681 attcagcgcg atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc
    19741 accgttcgtc agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt
    19801 ggtgcgcttc tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag
    19861 catcaaaatc aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc
    19921 agaaataaaa aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg
    19981 gtacttctcg cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt
    20041 caatagcctt gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt
    20101 tgagtcgcgc gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat
    20161 aattgcgaca ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatatt
    20221 tcgcattgcg gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc
    20281 gggttggcat tacgcagtca aacatatcga taccgcgacg tacgccttca accaggtctt
    20341 ccggtttacc aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc ggacacacat
    20401 gctccagaat acggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt
    20461 agccatcaaa gccgatatct accagacctt taacagatat atcacgtaaa tcttcgtaaa
    20521 tactgccctg aattatacca aataacgcat ttttgttacc aagactgtca aaacgctcac
    20581 ggctacgctt cgcccaacgc agagacatct ccatagagcg ttttgcgtaa tcccagtcag
    20641 caggatacgg cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa
    20701 tctccatcga cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt
    20761 gaacgccctg ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg
    20821 agtcggtaag aatcggtcct ttccactgca taaaatcgtg catatcgccg tgcagtttca
    20881 tgatttcctg gcccgggcgc agccacaggt ggaaagtgtt gccgaggata atttgcgcgc
    20941 cagtggcttc aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca
    21001 taaaacaagg cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac
    21061 cgtcggtggt gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa
    21121 cgtttaaacc agcgccgcgg aattactccc cgacgcgctc attaattgcc tgcggattgt
    21181 acgtgataaa catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt
    21241 tataggcatt catggtgtgt tgataaccgg caaaggccga aaccagcata atcagcgtcg
    21301 actctggcaa gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat
    21361 agataaagat ttgggtatcg tcaaagaacg gttcgataag atcgtttttt gccgcctgtg
    21421 ccgcgctttc cagcgaacgt actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg
    21481 ctttcgccgc cagtaccgcg tctaccacat cctgcggtac ttcagcgtat tccgagtgca
    21541 tgatgtgatc ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca
    21601 acgtcacaaa cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggctcgtcaa
    21661 aatgcagacc agcggtcggg gctgcaaccg cgcccggttt ttcgctataa acggtttgat
    21721 aaagttcgcg gtcagcgtct tcgtccggac gatcgatata cggcggcagc ggcatatggc
    21781 cgatgctgtt gaggatatcc agtaccgagc gttcatcatt aaattcgact tcaaacagtg
    21841 cgccgtggcg cgcggtcatt gttgcgttga tactttcgtc atcgcccagc aacagttctg
    21901 cgccaggttt tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca
    21961 tccgctcaac cagcacttca atcttaccgc cgctggcttt acgcccaaac agacgcgccg
    22021 ggatcacgcg ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat
    22081 cggtgaaagt accgtgcgtc agcgcacccg tcggcccgtc cagcgacagt aaacgacagc
    22141 tactgcgttc aggcatggga tagtgggcaa tcagggattc gggcaattca aaggagaaat
    22201 cggtaacgcg catgacactg actcagacta aaataagagt cgggtagtct agtgccgggg
    22261 cgctctccct gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct
    22321 cacctgcatt tagcccatct cgcggaaagc tcgctttctg gcaatttact ggctgatttc
    22381 gtacgcggaa atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat
    22441 cgacgtattg actgacaatc tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga
    22501 aacgcgccgc gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgcca
    22561 ctggtcgcag ctgtcgccgg actttccgct acaggaattt gtctgttatg cccgcgagca
    22621 agtgatgacg attttgccgg actcaccgcc acgttttatc aatctgaaca attacttgtg
    22681 gtcagagcag tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg
    22741 catggcaagc cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc
    22801 tcattatgac gccctcgaaa cccgcttctg gcagttttat ccgcggatga tggcgcaggc
    22861 gtcacacaag gcgttataaa acgttagttc atccataccg tagccgaaat ggcaggcaaa
    22921 gccagaatcc cgtcagtcag ttgcccatgc ccttctttgc attgccattg cacggcattg
    22981 agaaacggtg acgcgggtag caccacttca ctggcctcgc cacggttgat tgccaccagt
    23041 acacgttgct gattcagcac gcggacaaat accactacgt tatcttccgc atacagcacc
    23101 tgacagccgc cacgacgcag cgcctgactt ttcttacgca gcacaatcat tcgccggtac
    23161 agcgcgaata acgccgtatc ctgcttttcc acctgccagg ggaacggttt acggcaagac
    23221 ggatcgtttt taccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc
    23281 caggtgaaca gccagaccac cgccagcggc aggcgcgcaa tatcccgacc gagcagcgtt
    23341 ttaaatcgcg cagtatcgtg gctatcgagc tgattaaaca tacgtaattg ttgttgatga
    23401 gaaagccctg cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc
    23461 ggatcataag agatgtcggt attggcaaga aatccccaca acgggaatgt gaagccacga
    23521 tagttcatgg cagcatcttc cacatcggcc tgtaaccatt gccgtgcatc gccaaaatgt
    23581 tcgccgacaa tatacgcttc cgactgggtt tctttcgccg cttcggtgat cccggcaacg
    23641 tgctgcatat tattgcgcgc cccacccgcc tcccccagca tatgcaccac atccagccgc
    23701 cagccgtcca tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga
    23761 taaatttcat tcaccagact ttccgactga taatccagct tcggcaagct ggcatagcca
    23821 agccagtcga gcgccgtgcc atcatcacta aacaagtacc agtcgcgcca gggcgattca
    23881 gagttgtgac aagctccccc cgtgccaaga ttgtgcctgt ca
//
LOCUS       DADLTI010000053        23695 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLTI010000053 DADLTI010000000
VERSION     DADLTI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.053"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            581..2509
                     /gene="thrS"
                     /locus_tag="JN755_001880"
     CDS             581..2509
                     /gene="thrS"
                     /locus_tag="JN755_001880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7380693.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2513..3055
                     /gene="infC"
                     /locus_tag="JN755_001881"
     CDS             2513..3055
                     /gene="infC"
                     /locus_tag="JN755_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7380694.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3152..3349
                     /gene="rpmI"
                     /locus_tag="JN755_001882"
     CDS             3152..3349
                     /gene="rpmI"
                     /locus_tag="JN755_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7380695.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3402..3758
                     /gene="rplT"
                     /locus_tag="JN755_001883"
     CDS             3402..3758
                     /gene="rplT"
                     /locus_tag="JN755_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7380696.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3881..3925
                     /locus_tag="JN755_001884"
     CDS             3881..3925
                     /locus_tag="JN755_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7380697.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4208..5191
                     /gene="pheS"
                     /locus_tag="JN755_001885"
     CDS             4208..5191
                     /gene="pheS"
                     /locus_tag="JN755_001885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7380698.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5206..7593
                     /gene="pheT"
                     /locus_tag="JN755_001886"
     CDS             5206..7593
                     /gene="pheT"
                     /locus_tag="JN755_001886"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7380699.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7598..7897
                     /gene="ihfA"
                     /locus_tag="JN755_001887"
     CDS             7598..7897
                     /gene="ihfA"
                     /locus_tag="JN755_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7380700.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7998..8978
                     /gene="btuC"
                     /locus_tag="JN755_001888"
     CDS             7998..8978
                     /gene="btuC"
                     /locus_tag="JN755_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7380701.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9041..9592
                     /gene="btuE"
                     /locus_tag="JN755_001889"
     CDS             9041..9592
                     /gene="btuE"
                     /locus_tag="JN755_001889"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7380702.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9592..10341
                     /gene="btuD"
                     /locus_tag="JN755_001890"
     CDS             9592..10341
                     /gene="btuD"
                     /locus_tag="JN755_001890"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7380703.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10419..10883
                     /locus_tag="JN755_001891"
     CDS             10419..10883
                     /locus_tag="JN755_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7380704.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11130..11832
                     /gene="ydiV"
                     /locus_tag="JN755_001892"
                     /pseudo
     CDS             11130..11832
                     /gene="ydiV"
                     /locus_tag="JN755_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11896..13299
                     /locus_tag="JN755_001893"
     CDS             11896..13299
                     /locus_tag="JN755_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7380705.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13336..13527)
                     /gene="hemP"
                     /locus_tag="JN755_001894"
     CDS             complement(13336..13527)
                     /gene="hemP"
                     /locus_tag="JN755_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7380706.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13659..14705)
                     /gene="aroH"
                     /locus_tag="JN755_001895"
     CDS             complement(13659..14705)
                     /gene="aroH"
                     /locus_tag="JN755_001895"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7380707.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14862..15695)
                     /gene="ppsR"
                     /locus_tag="JN755_001896"
     CDS             complement(14862..15695)
                     /gene="ppsR"
                     /locus_tag="JN755_001896"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7380708.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16027..18405
                     /gene="ppsA"
                     /locus_tag="JN755_001897"
     CDS             16027..18405
                     /gene="ppsA"
                     /locus_tag="JN755_001897"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7380709.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18462..20162)
                     /gene="fadK"
                     /locus_tag="JN755_001898"
                     /pseudo
     CDS             complement(18462..20162)
                     /gene="fadK"
                     /locus_tag="JN755_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20165..20458)
                     /locus_tag="JN755_001899"
     CDS             complement(20165..20458)
                     /locus_tag="JN755_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7380710.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20455..21744)
                     /locus_tag="JN755_001900"
     CDS             complement(20455..21744)
                     /locus_tag="JN755_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7380711.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21800..22738)
                     /locus_tag="JN755_001901"
     CDS             complement(21800..22738)
                     /locus_tag="JN755_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7380712.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22758..23522)
                     /locus_tag="JN755_001902"
     CDS             complement(22758..23522)
                     /locus_tag="JN755_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7380713.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5846 a         5780 c         6246 g         5823 t
ORIGIN      
        1 gtgcaatcta tgaggagact tataatgagt tctgataatg atatcgtttt gactcataat
       61 ctattcgcct tatatatatc tggaggtttt gaggatgatg attaattatt atcgccagaa
      121 cgaaatgtgc acgaaaaatt gttataagaa ttgtgtcact tttatcattg tgatatgacc
      181 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      241 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      301 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      361 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      421 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      481 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
      541 gatctttcgt gtgggtcacc actgcaaata aggatataaa atgcctgtta taactcttcc
      601 tgatggcagc caacgccatt acgaccacgc tgtaagcccc atggatgttg cgctggacat
      661 tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc
      721 ttgcgatctg attgaaaacg acgcacaact gtcgatcatt accgccaaag acgaagaagg
      781 tctggagatc attcgtcact cctgtgcgca cctgttaggg catgcgatta aacaactttg
      841 gccgcatacc aaaatggcaa tcggcccggt tattgacaac ggtttttatt acgacgttga
      901 tcttgaccgc acgttaaccc aggaagatgt cgaagcactc gagaagcgga tgcatgagct
      961 tgctgagaaa aactacgacg tcattaagaa gaaagtcagc tggcacgaag cgcgtgaaac
     1021 tttcgccaac cgtggggaga gctacaaagt ctccattctt gacgaaaaca tcgcccatga
     1081 tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt
     1141 accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg cttactggcg
     1201 tggcgacagc aacaacaaaa tgttgcaacg tatttacggt acggcgtggg cagacaaaaa
     1261 agcacttaac gcttacctgc agcgcctgga agaagccgcg aaacgcgacc accgtaaaat
     1321 cggtaaacag ctcgacctgt accatatgca ggaagaagcg ccgggtatgg tattctggca
     1381 caacgacggc tggaccatct tccgtgaact ggaagtgttt gttcgttcta aactgaaaga
     1441 gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac
     1501 cggtcactgg gacaactaca aagatgcaat gttcaccaca tcttctgaga accgtgaata
     1561 ctgcattaag ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc
     1621 ttatcgcgat ctgccgctgc gtatggccga gtttggtagc tgccaccgta acgagccgtc
     1681 aggttcgctg catggcctga tgcgcgtgcg tggatttacc caggatgacg cgcatatctt
     1741 ctgtactgaa gaacaaattc gcgatgaagt taacggatgt atccgtttag tctatgatat
     1801 gtacagcact tttggcttcg agaagatcgt cgtcaaactc tccactcgtc ctgaaaaacg
     1861 tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg cgctggaaga
     1921 aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc cgaaaattga
     1981 atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac agctggactt
     2041 ctccttgccg tctcgtctga gcgcctccta tgtgggcgaa gacaacgagc gtaaggtacc
     2101 ggtaatgatt caccgcgcaa ttctggggtc gatggagcgt ttcatcggta tcctgaccga
     2161 agaattcgct ggtttcttcc cgacctggct tgcgccagtt caggttgtta tcatgaatat
     2221 taccgattca cagtctgaat acgttaacga attgacgcaa aaactatcaa atgcgggcat
     2281 tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg agcacacttt
     2341 gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatcag gcaaagttgc
     2401 cgttcgcacc cgccgtggta aagacctggg aagtatggac gtaaatgaag tgatcgagaa
     2461 gctgcaacaa gagattcgca gccgcagtct taaacaattg gaggaataag gtattaaagg
     2521 cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga
     2581 agttcgctta acaggtctgg aaggcgagca gcttggtatt gtgagtctga gagaagctct
     2641 ggagaaagca gaagaagccg gagtagactt agtcgagatc agccctaacg ccgagccgcc
     2701 ggtttgtcgt ataatggatt acggcaaatt cctctatgaa aagagcaagt cttctaagga
     2761 acagaagaaa aagcaaaaag ttatccaggt taaggaaatt aaattccgtc ctagtacaga
     2821 tgaaggcgac tatcaggtaa aactccgcag cctgattcgc tttctcgaag agggtgataa
     2881 agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa tcggtatgga
     2941 agtgcttaat cgcgtgaaag acgatttgca agaactggca gtagtcgaat ccttcccaac
     3001 gaagatcgaa ggccgccaga tgatcatggt gctcgctcct aagaagaaac agtaaggcct
     3061 tcaagtagca ctatctgtgg agcctttggg cttcacaggc attgttcgcc tttgtttcgt
     3121 ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa attaagaccg tacgcggtgc
     3181 tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg ctaacctgcg
     3241 tcacattctg actaaaaaag cgaccaaacg taaacgtcac ctgcgtccga aagccatggt
     3301 ttccaaaggc gatctgggct tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt
     3361 ttttaacttt ttaattagaa tatagataca ggagagcaca tatggctcgc gtaaaacgtg
     3421 gtgttattgc acgcgcacgt cacaagaaaa ttttgaaaca agctaaaggc tactacggtg
     3481 cgcgttctcg cgtataccgc gttgccttcc aggctgttat caaagctggt cagtatgctt
     3541 accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg
     3601 cagcagcacg tcagaacggt atttcttaca gcaaattcat caatggcctg aaaaaagcct
     3661 ctgttgaaat cgaccgtaag atcctggctg atatcgcagt attcgacaaa gtagcgttca
     3721 ccgctctggt tgaaaaagcg aaagcagctc tggcataagc cagttgaaag agggagctag
     3781 tctccctctt ttcgtttcaa cgccatcaaa acattgactt ttatcgccgt agccttttca
     3841 ataaaggtct tttgaagagt accaaaaggt aacgcaagca atgaatgctg ctattttccg
     3901 cttctttttt tactttagca cctgaatcca ggaggctagc gcgtgagaag agaaacggaa
     3961 aacagcgcct gaaagcctcc cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa
     4021 aagccagtct atcaggcgta attgtcgcag ccagtctgga cacggccagt gcggagaaac
     4081 cggagcgtac ttaagtacgt gaggatttcg agcactgccc gggaccaaaa tggcaagtaa
     4141 aatagcctga tgggataggc tctaagtcca acgaaccagt gtcaccactg acacaatgag
     4201 gaaaaccatg tcacatctcg cagaactggt tgccagtgcg aaggcggcca ttagccaggc
     4261 gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat ttgggtaaaa aagggcactt
     4321 aacccttcag atgacgaccc tgcgtgagct gccgccagaa gagcgtccgg cagctggtgc
     4381 ggttatcaac gaagcgaaag agcaggttca gcaggcgctg aatgcgcgta aagcggaact
     4441 ggaaagcgct gcactgaatg cgcgtctggc ggcggaaacg attgatgtct ctctgccagg
     4501 tcgtcgcatt gaaaacggcg gtctgcatcc ggttacccgt accatcgacc gtatcgaaag
     4561 tttcttcggt gagcttggct ttaccgtggc aaccgggccg gaaatcgaag acgattatca
     4621 taacttcgat gctctgaaca ttcctggtca ccacccggcg cgcgctgacc acgacacttt
     4681 ctggtttgac gctacccgcc tgctgcgtac ccagacctct ggcgtacaga tccgcaccat
     4741 gaaagcccag cagccaccga ttcgtatcat cgcgcctggc cgtgtttatc gtaacgacta
     4801 cgaccagact cacacgccga tgttccatca gatggaaggt ctgattgttg ataccaacat
     4861 cagctttacc aacctgaaag gcacgctgca cgacttcctg cgtaacttct ttgaggaaga
     4921 tttgcagatt cgcttccgtc cttcctactt cccgtttacc gagccttctg cagaagttga
     4981 cgtcatgggt aaaaacggta aatggctgga agtgctgggc tgcgggatgg tgcatccgaa
     5041 cgtgctgcgt aacgttggca tcgacccgga agtttactct ggtttcgcct tcgggatggg
     5101 gatggagcgt ctgactatgt tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa
     5161 cgatctgcgt ttcctcaaac agtttaaata aggcaggaat agattatgaa attcagtgaa
     5221 ctgtggttac gcgaatgggt gaacccggcg attgatagcg atacgctggc gaatcaaatc
     5281 actatggcgg gcctggaagt tgacggtgta gaaccggttg ccggtagctt ccacggcgtg
     5341 gtcgttggtg aagtggttga gtgtgcgcag catccgaacg ctgacaaact gcgtgtgaca
     5401 aaagtgaatg tcggcggcga tcgcctgctg gacatcgtct gcggtgcgcc aaactgccgt
     5461 cagggcctgc gtgtggcggt agctaccatt ggtgctgttc tgccgggtga tttcaaaatt
     5521 aaagcggcga aactgcgtgg cgaaccgtct gaagggatgc tgtgctcctt ttctgagctg
     5581 ggaatttctg acgatcacag tggcattatc gaactgcctg cggatgcgcc gattggcacc
     5641 gacatccgcg aatacctgaa actcgatgat aacacgatcg aaatcagcgt gacaccaaac
     5701 cgtgccgact gcttaggtat cattggtgtt gcgcgtgacg ttgccgtgct gaatcagctg
     5761 ccgctggttg aaccggaaat cgttccggtt ggtgcgacca tcgacgacac gttgccgatt
     5821 acagtcgaag cgccggaagc ctgcccgcgc tatcttggcc gtgtggtaaa aggcattaac
     5881 gttaaagcgc caactccgct gtggatgaaa gaaaaactgc gtcgttgcgg gatccgttct
     5941 atcgatgcag ttgttgacgt caccaactat gtactgctcg aactgggcca gccgatgcac
     6001 gctttcgata aagatcgcat tgaaggcggc attgtggtgc ggatagcaaa agagggcgaa
     6061 acgctggtgc tgcttgatgg cactgaagcg aagctgaatg ctgacactct ggtcatcgcc
     6121 gaccacaaca aggcgctggc gatgggcggc atctttggtg gcgaacactc tggcgtgaat
     6181 gacgaaacgc aaaacgtctt gctggaatgc gctttcttca gcccgctgtc tatcaccggt
     6241 cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc gttatgagcg tggcgttgat
     6301 ccggtactgc agcacaaagc gatggaacgt gcgacccgtc tgctgatcga catctgcggt
     6361 ggtgaggctg gcccggtaat tgatatcacc aacgaagcaa cgctgccgaa gcgtgcaacc
     6421 atcactctac gtcgtagcaa actggatcgc ctgataggcc atcatattgc tgatgagcag
     6481 gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg aaggcaaaga cgaatggcag
     6541 gcagttgcgc cgagctggcg tttcgatatg gagattgaag aagatctggt cgaagaagtc
     6601 gcgcgtgttt acggctacaa caacatcccg gatgagccgg tacaggcaag cctgattatg
     6661 ggtactcacc gtgaagctga cctgtcgctc aagcgcgtga aaacgctgct caacgacaaa
     6721 ggctatcagg aagtgatcac ctacagcttc gttgatccga aagtgcagca gatgatccat
     6781 ccaggcgttg aagccttact gctggcaagc ccgatctctg ttgaaatgtc agcaatgcgt
     6841 ctttctctgt ggaccggcct gctggcaacc gtggtgtaca accagaaccg tcagcagaac
     6901 cgtgtgcgca ttttcgaaag cggtctgcgt ttcgtaccag atactcaggc accgttgggc
     6961 attcgtcagg atctgatgtt agccggtgtg atttgcggta accgttacga agagcactgg
     7021 aacctggcaa aagagaccgt tgatttctat gatttgaaag gcgaccttga atccgttctc
     7081 gacctgaccg gtaaactgaa tgaggttgag ttccgtgcag aagcgaatcc ggcactgcat
     7141 ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta ttggttttgt tggagttgtt
     7201 catcctgaac tggaacgtaa actagatctt aacggtcgca ctctggtgtt cgaactggag
     7261 tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg agatttctcg cttcccggcg
     7321 aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc ccgcagcgga tattttatcc
     7381 gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa acttatttga cgtgtaccgc
     7441 ggtaagggtg ttgcggaggg gtataagagc ctcgccataa gcctgatcct gcaagatacc
     7501 agccgtacac tcgaagaaga ggagattgcc gctaccgtcg ccaaatgtgt agaggcatta
     7561 aaagagcgat tccaggcatc attgagggat tgaacctatg gcgcttacaa aagctgaaat
     7621 gtcagaatat ctgtttgata agcttgggct tagcaagcgg gatgccaaag aactggttga
     7681 actgtttttc gaagagatcc gtcgcgctct ggaaaacggc gaacaggtga aactctctgg
     7741 ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg ggacgtaacc cgaaaacggg
     7801 cgaggatatt cccattacag cacggcgcgt ggtgaccttc agacccgggc agaagttaaa
     7861 aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct gatctaacta aaaaggccgc
     7921 tatgcggcct tttttctttt cactgtcgaa gagtcaccgt aaaatcaacg ccatgacact
     7981 tcagcagaac ggataccatg ctgacacttg cccgccaaca acagcgacaa aatattcgct
     8041 ggttattatg cctgtcagtt ttgatgctgc tggcgcttct cttaagcctt tgcgccggtg
     8101 aacaatggat ttcgccaggt gactggttta ctcctcgtgg cgaactgttc gtctggcaaa
     8161 ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc ggcgctggct atatccggcg
     8221 ctgtaatgca ggcgttgttt gaaaatcctc tggcagaacc tggactactt ggcgtctcta
     8281 acggcgcagg cgtggggctt atcgccgcgg tattgcttgg gcaagggcaa ctccccaact
     8341 gggcgctagg gctgtgtgcg attgctggcg cgcttatcat cactttaata ctcttacgtt
     8401 tcgcccgtcg tcatctttcg accagtcggt tattgctggc tggcgttgca ttagggatta
     8461 tctgcagcgc actaatgacg tgggctatct acttttccac ctctgttgat ttacgtcagc
     8521 tgatgtactg gatgatgggc ggttttggcg gcgtagactg gcggcaaagc tggctgatgc
     8581 tggcattgat ccccgtgttg ttgtggatct gttgtcagtc caggccgatg aatatgttag
     8641 cacttggcga gatctcggcg cggcaactgg gtttacccct gtggttctgg cgcaatgtgc
     8701 tggtggcagc gaccggctgg atggttggcg tcagtgtggc gctggcgggt gctatcggct
     8761 ttattggtct ggtgatccca catattcttc ggttgtgtgg tttaaccgat catcgcgtat
     8821 tacttcccgg ctgcgcgctg gcaggggcga gcgcattgct gctggccgat attgtagcgc
     8881 gcctggcatt agctgccgca gagctgccta ttggcgtggt caccgcaacg ttgggtgcgc
     8941 cggtgtttat ctggttattg ttaaaagcag gacgttagcc gcaaaaagac ggtctatgat
     9001 taaaagctaa gatttttact gaccacaccc aggagaaacg atgcaagatt ccattctgac
     9061 gaccgtagtg aaagatatcg acggtgaagt gaccacgctg gagaagtacg ccggtaatgt
     9121 gctgttaatt gtcaatgtcg cctcaaagtg tggcttaacg ccgcaatatg agcagttgga
     9181 gaatattcaa aaagcctggg ccgatcgagg ttttgtggtg ctgggattcc cgtgcaacca
     9241 gtttctggaa caagaaccgg gcagcgatga agagattaaa acttactgta ccaccacatg
     9301 gggggtgacg ttcccgatgt tcagtaagat tgaagttaat ggcgaaggac gccatccgct
     9361 gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg ccggaagaga gcggattcta
     9421 tgcccgtatg gtcagcaaag gccgtgcacc gctgtaccag gatgatattt tatggaattt
     9481 tgaaaaattc ctagttggca gggacggaaa agtcatccag cgtttttccc cggatatgac
     9541 gccggaagat cccattgtga tggaaagcat taaactggcg ttggcaaaat aatgtctatt
     9601 gtgatgcagt tacaggatgt tgcggaatct acccgcctgg ggccgctttc aggcgaggtt
     9661 cgggctgggg aaatcctgca cctggtgggg ccgaatggcg cgggtaagag taccttactg
     9721 gcgcgaatgg ccggaatgac cagcggtaag ggaagcattc agttcgcggg gcaaccactg
     9781 gaagcatggt ccgcaacaaa actcgcgctg catcgcgcct atctttcaca acagcagacg
     9841 ccgccgtttg caatgccggt ctggcactac ctgacactgc atcagcacga taaaacgcgt
     9901 accgaactac tgaatgatgt cgcaggggcg ctggctcttg atgacaaact cggtcgtagc
     9961 accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc ttgctgcggt ggtgttgcaa
    10021 atcacaccac aagccaatcc cgcaggccaa ttgctgcttc ttgatgagcc gatgaacagt
    10081 cttgatgttg cgcaacaaag tgcgttagac aaaattctga gcgcgctgtg tcagcaagga
    10141 ctggcgattg tgatgagcag tcacgatctc aaccacacgt tgcgtcatgc gcatcgggcg
    10201 tggttgctaa aaggtggaaa aatgctggcc agtggacgca gagaagaggt gctcacgccg
    10261 ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc tggatatcga aggtcacaga
    10321 atgctgattt cgaccatctg atttcattag tatcccgaaa atcgggtaat tacgacgcta
    10381 aattaatgca agaataaaaa acagaggaac cgtacggaat gcgtttctgc cttattttga
    10441 tcacagcact gtttctggcc gggtgtagcc accataaagc accgccgcca aatgccagac
    10501 tttctgattc gattaccgtt attgccggtt tgaacgacca gctacaaagc tggcatggca
    10561 cgccgtatcg ttatggtggc atgacgcggc gcggtgtgga ctgttcggga tttgtggttg
    10621 tgacgatgcg cgatcgtttc gatttgcatc tgccccgtga aaccaaacaa caagcctcta
    10681 ttggcacgca aattgataaa gacgagttgc tgcctggtga cctggtcttt ttcaaaacgg
    10741 gttccggaca aaatggtttg catgtaggta tttacgatac caacaaccaa tttatccacg
    10801 cctctaccag caagggagtg atgcgttcct cacttgataa tgtctattgg cagaaaaatt
    10861 tctggcaggc gagacgaatc tagtgtgtcg aaaaattgac ggcgatgata catcgccgtc
    10921 aatttactcc tgaatacagt agtattttgc agacaaaaat tccgtaaatg gattattcgt
    10981 ttataatcag gataatctaa aaaaaggccc cctgaaaaag tggttaacat ggaacaaatg
    11041 aaagttactc cttctgtaat tgttatgtct tatggcctca tcccttttag ccggatactg
    11101 aaaaacatcc ttcgagaggg acggttacca tgaagatttt tttggagaat ctttatcatt
    11161 ctgattgcta tttcctgccg ataagagata accagcaact ccttgtcggc gttgagttaa
    11221 ttacccattt ctccagtgaa gatggcacgg ttcgtatccc gacgagtcgg gttatagcgc
    11281 aacttaccga ggagcaacat tggcaattat tttcagagca gttggaatta ctaaaatcat
    11341 gccaacactt ttttattcaa cataaactct ttgcctggct taatttaacg ccacaagtgg
    11401 ctacattatt attagacagg gataattacg caggcgaact attaaaatat ccgtttattg
    11461 agctgttgat taatgagaat tacccgcatc tcaatgaagg aaaagataat cgcgatcttt
    11521 tgtccttatc gcaaatgtat ccccttgtgt taggcaattt gggagcaggt aatagcacaa
    11581 tgaaagcggt ttttgatggt cttttcaccc gcgtaatgct ggagaaagga acaaatcacg
    11641 catcgctcct tcgaaccgtt tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat
    11701 tgcatcatcg cggggggcat tgatacgcca gaaatattgg cacagattac ccccttcgat
    11761 ttccatgctc tccagggatg cctgtggcct gccgttccta taaatcagat aacgacgttg
    11821 gttcagcgat aaccctcctg tttgctggtg tttaagacga gagtaaccgt ctacactatc
    11881 aaacagatag gatctatgac cctgtctttt attacccgct ggcgcgatga attgccagaa
    11941 acctatacag cactttcccc tacgccttta aataatgccc ggctgatttg gcataatacc
    12001 gaactcgcta acacgctgag tattccatcg tcgctgttta aaaatgccgc aggtgtctgg
    12061 ggcggcgaaa ccctactgcc tggtatgtca ccactggcac aggtttacag tggtcatcag
    12121 ttcggcgtct gggcgggcca actgggtgat gggcgcggca ttttacttgg cgaacaactg
    12181 cttgctgatg gcactacaat ggactggcat ctgaaaggtg ctggcctgac gccttattcg
    12241 cgaatgggtg atggacgggc ggttttacgt tcgacgatac gagaaagtct cgccagtgag
    12301 gcgatgcatt atctgggcat tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg
    12361 ccagtgtatc gggaaacggt ggagccaggc gcgatgctga tgcgtgtggc accaagtcat
    12421 ctgcgctttg gtcatttcga acatttttac tatcgccgcg agccggaaaa ggttcgtcag
    12481 ttggctgact ttgccattcg tcattactgg tcacatcttg aagacgatga ggacaaatac
    12541 cgtctctggt ttaacgatgt tgtcgcacgt accgcgtcgt taattgccca atggcagacg
    12601 gtcggctttg cacatggggt gatgaatacc gacaacatgt cgctgctggg actgacgctt
    12661 gattacgggc cgtttggttt tcttgatgat tacgaacccg gttttatttg taatcactcg
    12721 gatcatcaag ggcgttatag ctttgataat caacctgctg tcgcgttgtg gaatttacag
    12781 cgtctggcgc agacattgtc accatttgtt gccgtagatg ccctgaatga ggccctggac
    12841 agctatcagc aggttttgtt gacgcattat ggacaacgga tgcggcagaa actgggcttc
    12901 atgacggagc aaaaagagga taacgcgcta ctgaatgaat tattcagtct gatggcgcga
    12961 gagcgcagcg attatacccg cacattccgc atgctgagtc tgaccgagca gcacagcgcg
    13021 gcgtcaccgc tacgtgatga gtttattgat cgtgcggcat ttgatgactg gtttgcccgt
    13081 tatcgggggc gtttgcaaca agacgaggtt agcgatagtg agcgtcaaca actgatgcaa
    13141 agcgttaacc cagctctggt attgcgcaac tggttggcgc aacgggcgat tgaagcggca
    13201 gaaaagggtg atatgatgga attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc
    13261 gacagagatg atgactatgt cagtcgtcca cctgactgag gtaaacggct ggaagtcagc
    13321 tgctcgagtt aaaggctact tagttaacag cagcttgcca gcctgagttt tacggagcag
    13381 atattcttgc ccgtcatgat cgataatcag tttgccatcc ggacctaaca gtgtctggct
    13441 ggtaattcgc cggatgggct gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt
    13501 gagttttctg ctatccgtat aacgcataaa aattaataat aattgatata acaatgattc
    13561 ctattatcaa tattatggca ggccagagca agcagtaatg tgttgagaag acaacaagtt
    13621 gcgcagaaat gcccatccca tcaggaatgg gcacgcattc agaagcgggt atctaccgca
    13681 aaggcgagtt tttcgaccag gcgttcggta tcttcccagc ccagacacgg gtcggtaatg
    13741 gattgaccgt aagtgagcgg ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa
    13801 ctttccgcca taattccagc aatctccgta gagccattgc ggatttgctg acaaatatcc
    13861 tcacaaactt ctaactggcg acggtgctgc ttctggcagt taccgtggct gaaatccacc
    13921 accagatgtt caggtaaatc aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg
    13981 gcatgataat tcggtttttt gccgccacgc ataataatgt ggccatacgg gttgccgctg
    14041 gtctgataga tggtcatctg accatttttg tctggcgaga ggaacatatg gctggcgcgg
    14101 gctgcgcgga tagcatccac ggcaatccgc gtgttgccat cggtaccatt tttaaaacct
    14161 accggacagg agagtgctga agccatttcg cggtggatct gactttcggt agtacgagcg
    14221 ccaatcgcgc cccaactgat taaatcagca ataaactgac cggtcaccat atcgaggaac
    14281 tcagtcgcgg tagggacacc cagctcattt acctgtaaaa gtaatttgcg cgccagctcc
    14341 agaccgtgat ttacccgata gctgccgttt aaatccggat cggagattag ccctttccag
    14401 ccgacaacag ttcgtggttt ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg
    14461 tactggttgc gcagcgactg cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg
    14521 atcgagcagg ggccaataat gaccaacagt cgcttatctt caccattcag tattttttca
    14581 attctgcggc gggagtcggt gacatgggtg gcgacgccag gcgttacggg ataccgtagc
    14641 gcgagttcgg cgggcgttac caggctctca atacgcgcag tacggagttc gtcagttctg
    14701 ttcattacaa gtctcagaaa tttgttgctt ctacggcagg actaccggaa agtgatgcgc
    14761 atcacgataa accaatcgca gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg
    14821 gttagcatga taacaaaaaa acaaaccaat acactagttc tctagtacat tcggcgacta
    14881 aggcccatga tatcgaggat cttggtggca atctcttcta ccgaataatt ggtactgtta
    14941 atccacggga tctgattttt acggtacaag gcttccactt ccgcgacttc catcctgcac
    15001 tgacgaagcg aggcatagcg actgttctcc cgacgttcct cgcgaatcgc cgccagacgt
    15061 tccgggtcga tagtcaggcc gaacaattta tgctgaagcg gtttgagcga cgcgggtagc
    15121 accagattat ccatatcgtc ggcaataaag gggtagtttg ccgcgcggat accaaattgc
    15181 attgccagat acagactggt gggggtttta ccacagcgag aaacaccgag caggatcacc
    15241 tgagcctgat ccagattgcg caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc
    15301 gccgcaatgc gcgcatcata tttattgaga ttattagggt taaggccatg ggtacgatga
    15361 gcaatcggcg ttggatccag tttcatctct tgttgtagcg gggcaaccag cgcctgaacg
    15421 atatcctggc aaaagccttc actttgcaag atgatggcgc gaatctccgg caacacgatg
    15481 gagtagaaga ccagcgggcg cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc
    15541 actgcccgtg cacggctctc attttcgaca aacggcagcg tgatgctgct gatagtgacg
    15601 ggaaattgtg acattactgc gtgtcctaat acctccgcag ttattgccgt accatcagaa
    15661 atataaaaaa cgtggcgatc aacagcatta tccattttgt tcttcccgtg aagcagacat
    15721 aattctatga aagcataaat taaaagcgca cagaagcgta ggacgtaatg tctggtttat
    15781 aaaatgaacc ttcattttat tttttatgaa aacagcattt catttttatg gtttcgttta
    15841 taccgatggt ttatgtggaa attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt
    15901 cttaaaaagt aaaaaaatat atttgcttga acgattcacc gtttttttca tccggtaaaa
    15961 tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat
    16021 tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt ggtataacca actcggcatg
    16081 aatgatgtag acagggttgg gggcaaaaat gcctccctgg gtgaaatgat tactaatctt
    16141 tccggaatgg gtgtttccgt tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag
    16201 tttctggacc aaagcggcgt aaaccagcgc atttatgaac tgctggataa aacggatatt
    16261 gacgatgtta ctcagcttgc gaaagcgggc gcgcaaatcc gccagtggat tatcgacact
    16321 cccttccagc ctgagctgga aaacgccata cgcgaagcct atgcacagct ttccgccgat
    16381 gacgaaaacg cctcttttgc ggtacgctcc tccgccaccg cagaagacat gccggacgct
    16441 tcctttgccg gtcagcagga aaccttcctc aacgttcagg gttttgacgc cgttctcgtg
    16501 gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac
    16561 cagggttacg atcaccgtgg cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc
    16621 gacctcgcat cttctggcgt gatgttctcc attgataccg aatccggctt tgaccaggtg
    16681 gtgtttatca cttccgcatg gggccttggg gaaatggtcg tgcagggtgc ggttaacccg
    16741 gatgagtttt acgtgcataa accgacactg gcggcgaatc gcccggctat cgtgcgccgc
    16801 accatggggt cgaaaaaaat ccgcatggtt tacgcgccga cccaggagca cggcaagcag
    16861 gttaaaatcg aagacgtacc gcaggaacag cgtgacatct tctcgctgac caacgaagaa
    16921 gtgcaggaac tggcaaaaca ggccgtacaa attgagaaac actacggtcg cccaatggat
    16981 attgagtggg cgaaagatgg ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa
    17041 accgtgcgct cacgcggtca ggtcatggag cgttatacgc tgcattcaca gggtaagatt
    17101 atcgccgaag gccgtgctat cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat
    17161 gacatcagcg aaatgaaccg catcgaacct ggcgacgtgc tggttactga catgaccgac
    17221 ccggactggg aaccgatcat gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt
    17281 acctgtcacg cggcgatcat cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga
    17341 gatgcaacag aacggatgaa agacggtgag aacgtcactg tttcttgtgc cgaaggtgat
    17401 accggttacg tctatgcgga gttgctggaa tttagcgtga aaagctccag cgtagaaacg
    17461 atgccggatc tgccgttgaa agtgatgatg aacgtcggta acccggaccg tgctttcgac
    17521 ttcgcctgcc tgccgagcga aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt
    17581 atgattggcg tccacccacg cgcactgctt gagtttgacg atcaggaacc gcagttgcaa
    17641 aacgaaatcc gcgagatgat gaaaggtttt gattctccgc gtgaatttta cgttggtcgt
    17701 ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt
    17761 ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca
    17821 gatgaagaga acccgatgct cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc
    17881 cgcgactgct tcgcactgga gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg
    17941 accaacgttg agatcatgat cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt
    18001 gaagaactgg cgcgtcaggg gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg
    18061 tgtgaaatcc cgtccaacgc cttgctggcc gagcagttcc tcgaatattt cgacggcttc
    18121 tcaattggct caaacgatat gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg
    18181 gtgtctgaat tgttcgatga gcgcaacgat gcggtgaaag cactgctgtc gatggctatc
    18241 cgtgccgcga agaaacaggg caaatatgtc gggatttgcg gtcagggtcc gtccgaccac
    18301 gaagactttg ccgcatggtt gatggaagag gggatcgata gcctgtctct gaacccggac
    18361 accgtggtgc aaacctggtt aagcctggct gaactgaaga aataaaataa atccccggcg
    18421 gcgttcagtc gccggggtta tgtgatcccc gaagatgaaa cttattcaat ctcttcacag
    18481 acatcctgcg ttaatcgccg tataatatct ttccttaaca aaaacttttg tattttatct
    18541 gaggcagtgc gcggtagttt ttcgattacc acgatatgtt caggatattt atattttgcg
    18601 acccgtttac ggctaaaaaa agccaccatc tcttccagcg ataatgaatg atgcggcgct
    18661 ttctgcacga cataagcgca tgatcgttca cctaaacgtt catcgggcat tgcaaccaca
    18721 caggcatcgt gaattttagg atgctgcaat aaaatatctt ccacttcacg gctgctaata
    18781 ttttcgccgc cgcggacaat aatatctttt ttgcgtccgg taatttttat atagccagcc
    18841 tcatccatac ggcagagatc gccgctgtaa taccagcctt cttcatccag cgcacgggcg
    18901 gttaattcag gttcatcaaa ataccccata aacacattgg ggccacgcga tgtttcttca
    18961 ccttcgcaac ctggcggtaa ggtcttgcgt gcgtcatcga ccactttaat ctctacacct
    19021 gcggcagcgt aaccatcggt atgcataaag cgcgacaaag aattatcgag attcaccacc
    19081 gcatgcggcg aactttctgt ggaaccataa acacttaata atttaatgct gcgctgctgg
    19141 cattcgcgcg ccactttttt ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct
    19201 gaaaggtctg cgggttgttt ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg
    19261 cctagcatac aggtgcaacg ctgctgctca agcagcgcga gacacgcatc aggagtgaaa
    19321 atatctaaca acacgctgcg agcgccaatt aagaatggtg ccgttacgcc atgcagaaag
    19381 cccgttgcgt gaccaagtgg cgcaggcatc ataaagacat ccagccaggt cagattcagc
    19441 cgcgcgcaat aagcccgctc actggcgaga atattgttat gcgttagcat cacgcccttt
    19501 ggcagaccct cggttccgga ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt
    19561 atcgccgtgg tcagtgggat attgtcggcg ataatctgac ttaatgagag ggaagaggtg
    19621 gcgggagcca gtttgtccac gccgacaatt tgttgtagtt gtggaagctg attttgcagc
    19681 ggcaggatta aatctaccgg acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt
    19741 gcctgacact tattgagcac ccacaccagt tctgcttccc gccaggaagg caacagcggc
    19801 acggaaaccg caccgatttt caggcaggca agatagataa cggtaaattc acaccagcca
    19861 ggcagttgaa atgcgatgcg atcgcctgat tcaataccct tcgctaacat ccagtttgcc
    19921 agacagctcg cggcgtgatc gagcgcgcta taggtgtacg atgcaccatg attatcgacc
    19981 acggcaattt tgtctggcat cgcacgagcg gtctgctgtc agtaatcggc cagtgaagca
    20041 tcgccccata gcccttgctg acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc
    20101 actttcattt tggactctcg aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc
    20161 atgattagcc gtagcgaaaa tcaataccga aggtgcctgc gggatattgc cactgttcga
    20221 gaatagtgtt accgcacagc acccgacagg tgccgcactc cagacaaccg gcgggatcaa
    20281 aatgaatgtt tcctacgtca tcctgcttat aaagtccggc agggcaggct ttcattaatt
    20341 tacggaattc attgatatcg ggattttccg tcagaatgat atgcgggtgg ccctcatcaa
    20401 catggaattt attgacgcct aatttgatgt cgacgttaac cgtagcgttc tggctcatag
    20461 cgcggttgct cccttaatgc catctttcag caagttgatc agcccaattt tcttcgcgtg
    20521 tcccatgatc attttgcgta ccggctggtt tggtttgccg tcaatggtga acatctcgtt
    20581 catgatgtcg gctaccattc gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc
    20641 cgggatcttg cgaaaatgct gcatatcacg catgacgcag ctttgttcca gctcacgttt
    20701 gtattgcgcc agactgttgg cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt
    20761 ggcggcagcc tgagccgatg caatggctaa atccatgccg cggaccgtaa aacccaaatt
    20821 caggcagaag cctgcggcgt caccaacgat catcacgccc tcgttaacca gttgcggcac
    20881 cattgccaga ccgccttccg gcaccatatg cgcggaatat tcaagcagtt tgccgccgct
    20941 aatcagcggg cgaatggcgg ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt
    21001 ttgcgcatgg gcgatatcac ccaatccaca aaccagcccc aaggatatgg aatccttgtt
    21061 ggtatagata aatcccccgc ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc
    21121 ggcaccttcc tcgcccgtaa tattaaagcg atcgttgatc tgttctggtg tgaggccaat
    21181 aacctcttta acaccaacag cgtaatgatg cggatcggaa gcgggaacca ttcccagcga
    21241 gcggccaagc attgagttaa cgccatcagc cagaatcacc acattcgctt cgagaatatc
    21301 atccccagcc tgcacgccag tgaccttgtt tccttcacga accagcgcgt cgacgcgaac
    21361 gcctgggata aactgcgcgc ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg
    21421 attacgcaat acggtataag atgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc
    21481 gagggtaacg gcgctttctt cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc
    21541 taccggcgct gatactgcaa agcctggaat gattgcttca agtgtgtggg cataaagacg
    21601 cccgccggtc atgtttttac atccggcact gtcgccgcgt tctatcacca ggacatccag
    21661 cccggctcgt gccatgacca gtgcggcaac gctaccagca acgcccgcac cgaccacaat
    21721 ggcatcaaat ttgtcatccg acatgcgtta tgctcccttc ccgctgcgtt tacagcggga
    21781 gatgtggttg aaaggggaat tagcggctca actggctaat cagggcaggg acgactttgt
    21841 agatatcgcc caccagaccg tagtcggcat agttgaagat tggcgcattt ttatctttat
    21901 tgatggcgac aatcactttt acgccgttgc cgccaaccat atgttggatc tgcccggaga
    21961 tcccctgcgt caggtagaga tcggatttca gcaacacgcc ggagacaccg atataacgtt
    22021 cacgctccat ccagttctcg ccttcggcaa ttggacgtga acagccgact tcagcattca
    22081 gcaccgccgc cagttcgtgg accattttca ggtcatcctg cgccgccaga ccacgaccga
    22141 cgccaaccac acgttttgct ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt
    22201 cgcgacagag aatttcatga cgcggagcaa cataaggtac cgtttctgtc gggcactgat
    22261 gagaggtatc actggtgcac ggttcctgaa caccgggtgc aagggaaatg atcgccagcg
    22321 ggctgttgat cttttcctga gcgaacgcta acccgccata catccggtgt tcggcgcaaa
    22381 tgtgaccatc gacaatatcc accgccgtgg catcgttcac cagcgccgca ttcagttgca
    22441 cacttaaccg tgctgccaga gctttaccac gtttcgtcgc ggccaacagc aacatagcgg
    22501 ggtgtttatc tttcagcagg gcagcaatgc tttcggcgta attttcagtg cgttgcagcg
    22561 cgtcgttttg ctcaagaaca taaatacatt ttggaccata aggcataact gcctgcgcct
    22621 ggtcggtatt ttgtacaatg gcatacacct gttggcccca ttgctgagcg ccgccaaaca
    22681 gttcagcata acgttcagga ttatcgctaa agacccagac gctgtttaat tgactcatcg
    22741 tttcttctcc gtaggcttca gttcagggct ttcttcagat gttccgccag ctcggcaatg
    22801 gcctccggcg aatcgttatc gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga
    22861 atgccgacca gttcagcaag tggcgcgctc tggctccagt caatatcact tgcctgccac
    22921 tgatttaccg gttttttacc cgcgccgaga atggctttca tcgaaggaat gcgtggcacg
    22981 ttaatatcgg aggtgacgca gagcacggct ggaacagaga gttcaataac ttcaacatca
    23041 tcttcaagcg tgcgttcaat caccagtgta ttgccctgac gctgaatagc actcactgca
    23101 ttaatcaccg gaagttgcag aatttctccg accagcaagc caacctgctg ggcataaagg
    23161 tcgccggaac cttcaccaaa gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc
    23221 agcgcttttg cggtatcgag aggcagtgca tgttcaagtt gcgcatcctg caccaaatac
    23281 aggctgtgcg gcccgcggga tagcacgtct ttgcgcactt tcgagttctg caacaatgag
    23341 ccgccaacgg tcagcgcggc tatctcatcg tcatctgttg cgagctggct tgcagcttca
    23401 atggcattga gatcgaactg gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat
    23461 tctggagtga caacaatgtc ctgttcttca ggcaccagct taaagcaggt tattattttc
    23521 atgatttctc ctgtgagtcg catacggaac catctccgat ggaaggtaaa aatggcctga
    23581 aaatagtgtg cgatagcggt gcgggggggt cgacggagca agctgttttc cttgccaaca
    23641 atcttgcaaa aaaaatgcga ttgctaaaac aaaagtgcaa tttgcggcaa tatag
//
LOCUS       DADLTI010000054        23149 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLTI010000054 DADLTI010000000
VERSION     DADLTI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.054"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JN755_001903"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JN755_001903"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7380714.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JN755_001904"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JN755_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7380715.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JN755_001905"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JN755_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7380716.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JN755_001906"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JN755_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7380717.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JN755_001907"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JN755_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7380718.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JN755_001908"
     CDS             5281..5739
                     /locus_tag="JN755_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7380719.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JN755_001909"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JN755_001909"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7380720.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JN755_001910"
     tRNA            6791..6875
                     /locus_tag="JN755_001910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JN755_001911"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JN755_001911"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JN755_001912"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JN755_001912"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7380721.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JN755_001913"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JN755_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7380722.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JN755_001914"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JN755_001914"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7380723.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JN755_001915"
     CDS             complement(10156..13899)
                     /locus_tag="JN755_001915"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7380724.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JN755_001916"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JN755_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7380725.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JN755_001917"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JN755_001917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7380726.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JN755_001918"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JN755_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7380727.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JN755_001919"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JN755_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7380728.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JN755_001920"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JN755_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7380729.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JN755_001921"
     CDS             complement(20438..21133)
                     /locus_tag="JN755_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7380730.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JN755_001922"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JN755_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7380731.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JN755_001923"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JN755_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7380732.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5798 a         6043 c         5570 g         5738 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tgatattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcgggg
//
LOCUS       DADLTI010000055        22780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLTI010000055 DADLTI010000000
VERSION     DADLTI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.055"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..1279)
                     /locus_tag="JN755_001924"
                     /pseudo
     CDS             complement(1..1279)
                     /locus_tag="JN755_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(1283..1567)
                     /gene="gatB"
                     /locus_tag="JN755_001925"
     CDS             complement(1283..1567)
                     /gene="gatB"
                     /locus_tag="JN755_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7380733.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(1598..2050)
                     /gene="gatA"
                     /locus_tag="JN755_001926"
     CDS             complement(1598..2050)
                     /gene="gatA"
                     /locus_tag="JN755_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7380734.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(2060..3322)
                     /gene="gatZ"
                     /locus_tag="JN755_001927"
     CDS             complement(2060..3322)
                     /gene="gatZ"
                     /locus_tag="JN755_001927"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY7380735.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTLADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            complement(3351..4205)
                     /gene="gatY"
                     /locus_tag="JN755_001928"
     CDS             complement(3351..4205)
                     /gene="gatY"
                     /locus_tag="JN755_001928"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY7380736.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(4513..5565)
                     /gene="fbaB"
                     /locus_tag="JN755_001929"
     CDS             complement(4513..5565)
                     /gene="fbaB"
                     /locus_tag="JN755_001929"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7380737.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            5719..6195
                     /locus_tag="JN755_001930"
                     /pseudo
     CDS             5719..6195
                     /locus_tag="JN755_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6192..7195
                     /locus_tag="JN755_001931"
                     /pseudo
     CDS             6192..7195
                     /locus_tag="JN755_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            7192..8157
                     /locus_tag="JN755_001932"
     CDS             7192..8157
                     /locus_tag="JN755_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7380738.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8131..8877)
                     /locus_tag="JN755_001933"
     CDS             complement(8131..8877)
                     /locus_tag="JN755_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7380739.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8929..9747)
                     /locus_tag="JN755_001934"
     CDS             complement(8929..9747)
                     /locus_tag="JN755_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7380740.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            complement(9812..10612)
                     /gene="thiD"
                     /locus_tag="JN755_001935"
     CDS             complement(9812..10612)
                     /gene="thiD"
                     /locus_tag="JN755_001935"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7380741.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(10609..11397)
                     /gene="thiM"
                     /locus_tag="JN755_001936"
     CDS             complement(10609..11397)
                     /gene="thiM"
                     /locus_tag="JN755_001936"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY7380742.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(11432..11528)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11620..11892)
                     /gene="rcnR"
                     /locus_tag="JN755_001937"
     CDS             complement(11620..11892)
                     /gene="rcnR"
                     /locus_tag="JN755_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7380743.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            12013..12855
                     /locus_tag="JN755_001938"
     CDS             12013..12855
                     /locus_tag="JN755_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7380744.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            13074..13412
                     /gene="rcnB"
                     /locus_tag="JN755_001939"
     CDS             13074..13412
                     /gene="rcnB"
                     /locus_tag="JN755_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7380745.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(13494..14527)
                     /locus_tag="JN755_001940"
                     /pseudo
     CDS             complement(13494..14527)
                     /locus_tag="JN755_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(14543..17023)
                     /locus_tag="JN755_001941"
     CDS             complement(14543..17023)
                     /locus_tag="JN755_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7380746.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(17039..17713)
                     /locus_tag="JN755_001942"
     CDS             complement(17039..17713)
                     /locus_tag="JN755_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7380747.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            complement(17794..18336)
                     /locus_tag="JN755_001943"
     CDS             complement(17794..18336)
                     /locus_tag="JN755_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7380748.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(18629..18910)
                     /locus_tag="JN755_001944"
     CDS             complement(18629..18910)
                     /locus_tag="JN755_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7380749.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(19172..20281)
                     /gene="apbC"
                     /locus_tag="JN755_001945"
     CDS             complement(19172..20281)
                     /gene="apbC"
                     /locus_tag="JN755_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7380750.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            20413..22446
                     /gene="metG"
                     /locus_tag="JN755_001946"
     CDS             20413..22446
                     /gene="metG"
                     /locus_tag="JN755_001946"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7380751.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            22587..>22780
                     /locus_tag="JN755_001947"
     CDS             22587..>22780
                     /locus_tag="JN755_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY7380752.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
BASE COUNT         5834 a         5749 c         5291 g         5906 t
ORIGIN      
        1 aattgcgccg ataataatga aaccgggaat attttgcggg gagaaaacct gaatcagtaa
       61 ccaggtaatg ggagaaccgc cctgatccat tgaagccacc atacccccgg cttttaacgc
      121 cccagcatta gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga tccacagggt
      181 gatgctcata atgatgacac ccgagattaa ggtgcggaac agatttccac gatgcaccgc
      241 gaccgccatc gccacgaaga agccgatagt ggcaagatcg ccaaacggca gtacctgatt
      301 acccggaaca caaacagcaa ttaaaatggt gagcggaata aaaatcaggc ttgccgatac
      361 caccgccgta tgccccagca gtaatgcggg atccaggcca atcaggaact cctgaccgcc
      421 gaacttcgcc tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac catccatgat
      481 gggtttaatc atccgtggca tcagcagcat aactgccgcc gtttttaccg ccagctgcaa
      541 tacacctttg acatcgtaac ccgcgaggat gccgataatc agccccatca caaaacccac
      601 ggtgacaggt tcgccaaaag ggccaaattt gcgctgaata tcgtcggcgc taaatttaat
      661 tcggttaacg cctgggattt tctcgatgat agcatcgacc agcaccgcaa tcggccccat
      721 atacgccgac gtaccgtgcg gaatagcaat accttccagc tcaaagaaat ttcgggtatc
      781 gcgggcaaac cagtcgccga gcttataaac aaacgccgcg tgaattacca cacccgccat
      841 ccctatcatc catgaaccgg ttgccaggtg cagcaacgct gccggtgaag gtcatatgcc
      901 agatattcca gatatcaaca tttaccaccc gcgtcatacg ggtcagtaac atcgccacgt
      961 taaccaatat cgcaatcgga atcgccacca gcgcaatttg cgacgtccag gtcattggtg
     1021 aagagcccgg ccagccgaca tcgaccacat gcagattcag gtcgaaattt tccgccatcg
     1081 ctttcgccgc cggaccaatg gaatccagca ttaagccaat cacaaggcca atgccaacaa
     1141 agccaatccc gatatgcaga cccgctttaa agcaatcgcc tgccttcatg cctaatattt
     1201 tagaaaaaat aatgatgaca accggcagca tcactgtagg gccgaggtcg agaatataac
     1261 gcatgacttc tgaaaacata ggtcacccct gtaagatagt cagaatttta ttttgtaatg
     1321 cttcgatacc gacaccagaa acaaaaggca tgccgtgaac taacggaata tcgccaaaac
     1381 tacgatccac cctggcagtg gtacatatca aatgcacacc atccatatag gtttctattt
     1441 cattaacccg acactggatt aattcaacag gaatattatg actctgacac aactctttaa
     1501 tttcttccgc cgccatcgta gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc
     1561 gtttcataaa tagtcctttt tattgttaca gagggattta ttcgtctgaa tctaaaacat
     1621 actttgtgaa gtattccttt aactgagttt caggaagagt aattagtgtc tcgacgatat
     1681 cgggctgttg taacttgcca aataaacagc gtaaaagttt caattgctgc tgcggatttt
     1741 ccacaatcaa cgcaataacc aacgataccg ccacgtcgtt atcatcatcc gcttgctgaa
     1801 aatgaacttt atttgttggc cttaacagat aaatggctga cgacttagca tgaatcgcct
     1861 cacaatgcgg tattgcaatg gcgtgctgct caagcattat cccggtaggg aattctgctt
     1921 ctctggcaat taatgcctgt ggccaggtat catgaaccac acctttcgcg agcatctcat
     1981 taccgatata ggttaaaact tcgctacgat cgacaaaaga aattccgcta cgaacaaaca
     2041 ggttagtcat ataccgtcct tattccgcac agccgtagcg ataggcgcgc aaaacatcat
     2101 aaattttatt cataataagc tgatgcggca ttgctgataa ttccccggac tgaatgcgtt
     2161 caaattgttt gggaagatac tgactaatca tgcccagtgg gatgtccacg ccttgcagat
     2221 tcaccatcat cgtttcaacg ctatttttaa tccgactatg cggccagtaa taacgaatac
     2281 gatccgacag gctgtaacga atatccagta atgaatcgtt aaaacccgtg cgataatatt
     2341 ttttccagta ttgcggttcg tcgagcatca cttcttcaat taccgccagg caaccgctgc
     2401 gattttcggg ggcgataagt tcctgctcaa tttgtgccag tgcaaatatc gcctcgcgta
     2461 aagcaaaggt taatgcagga ccgactttca atattgcaaa gtgatcgcgg actaattccc
     2521 aataagccgt ccgggtctgg taatcggtag aatgtgcttc ataaaccatt cgggtgtttt
     2581 ctatccattg cgccagcgcc tgcgcttcct gcggctgata atggataata ttgctgtgat
     2641 caaattccac acccggctgc accacgatgg caatcacacg tgttaacgcc tctgtcagcc
     2701 cacgggcaat aaaggccttt tgatgcgtac gtaaagtatt ggcggcatct tcaacatggg
     2761 tgatgtgtac tgactgaatg gcgctggcct caccgcccgg aaccggaact tcagtgccaa
     2821 tgacatagct cagttgctca cgctggcaat ctgtcgccac actttccgca gcaaagcaaa
     2881 gcacagcagc tcgttccgca accgtttctg gtgctaacgg tatgggatcc cccgcgcagg
     2941 acattgacgc atcaagatga attttactga agccagcacg aacatatgct tttaccagct
     3001 cgacggattt ttccatcgcc gcatccgcat tttcttgctg ccagcagttt ggccccagat
     3061 gatcgccgcc gagaataatg cgttcgcgtg caaacccaac tttatcggca atcgtaaaaa
     3121 caaattcgcg aaagtctgcc agtgtcattc cggtataacc gccaaattga ttgacctggt
     3181 ttgacgttgc ttcaatcagc actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt
     3241 cgataaccaa cggatgggca gaacagactg aacatatgcc gatatgttca ccagctttat
     3301 gccgggcaat taacgttttc atgttttttc cttgttaaat ggcaggtgcg ttatgccctg
     3361 ccctcgcagc cacaatcggc aatcactttg ctcaccacat cgcgcattgc ggatttagcc
     3421 gactgcaaat aatcccgggg atcggtcgct tcggggtgct cggtcaggta attttttaac
     3481 gactgcgaga aggcattttt cagctccgtt gcaacgttga ttttgcatat ccccagtttg
     3541 atggtttgct gaatatcctt agtcgataat cctgacgcgc catgcagcac cagcggtaag
     3601 ttcacccact ggcgaatgtt ctccagtcga gaaaaatcaa gcaccggtgc gctggcatac
     3661 ataccgtgag ccgtgccgat ggcgaccgcc agggaatcaa tcccggttgc ctcggcaaat
     3721 tcacgcgcct gtgcggggtt ggtgtacaac gcatcggctt cattgacttg cacatcatct
     3781 tcctggccgc caagttgccc cagctccgcc tcgacgctga catcaaagcg atggcaaaaa
     3841 tccaccacct ctttgacccg tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc
     3901 atgactgagc gcacgccaga acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg
     3961 tcgagatgaa ttgccagtgg atggtgatat tgcttcgcca tcgcgctgac cagcgccaac
     4021 agattttctg taccagcatg agtaaatgtg ccaggcgttc cggcgatgat gaccggcgca
     4081 tgcaggttgg cagcggtttc caccaccact tgcatcgttt cgagattgtg aatattgaat
     4141 gccggaaccg cataaccgcc gcgctgtgcg ttgttcagca tctgctttgt cgataccacg
     4201 tacattttca tatcttgttg tttgctttcg atttcaaaaa ataatgaaat tatttgcttt
     4261 taaatgtcga gataacgatc acaaaaacga caatatgaaa attattcgag gagtgaaagg
     4321 aaaaaaaacg gcctcccgat agggaagccg tagcaaagtg cgcgtgtttt tatgccggat
     4381 gcggtgtaaa catacgtggc agtgctatgt aggcatgata agacgcttcc gcgtcgcatc
     4441 aggcataggt tgccggatgc agtgtaaacg ccttatccgt cctacggggt ggtgctgttc
     4501 gtatgaggcg tatcaggcaa tagtaatttt gctatcgaga taaacgtcct gcacggcatt
     4561 aatcagtttc acgccgtcag ccatcgattt cttgaacgcc ttacgtccaa gaatcagccc
     4621 cattccgcct gcgcgtttgt tgataaccgc agtacgcact gcatcgctga ggtcagtttc
     4681 accgcctgca gcaccgccgg agtttatcaa cccagcccgg cccatatagc agttagctaa
     4741 ctgataacgc accagatcaa tcgggttttc gctggttaac ttgctgtaca cgcgatcgtc
     4801 ggtataaccg taattaatcg ctttatagcc gccgttattt tccgccattt tttgtttgac
     4861 gatatctgca cctatggttg ccgccagatg gttcgcctga ccagtcaggt cggcggaaac
     4921 atggtaatca acgccatctt tcttaaaggc ggagtttcgc aaataggccc acagcactgc
     4981 caccatgccc agctcgtgcg cgcgttcaaa ggccgcagaa atttcttcaa tctggcgacg
     5041 tgactcttcc gagccaaaat agatagtcgc accaaccgcc accgcgccca tgttgaaggc
     5101 ctgctccacg ctggcataca gcgtttgatc gtaggtattc gggtaactta gcgtctcgtt
     5161 gtgattaagt ttgacgagaa atggaatgcg atgcgcatag cgccgcgata ccgacaccaa
     5221 cacgccgtaa gttgatgcca cacagttaca gcccgcttcg atcgccagtt caacaatgtt
     5281 tttcgggtca aagtagagcg ggttagcagc aaatgaagct ccggcagagt gctcaacgcc
     5341 ctggtcaacc ggcagaatag aaagatatcc tgtgccagcc agacgcccgg tgttgtacaa
     5401 cgtctgcata ttacgtaaca ccgctggcgg gcgattattg tcaatcatca cgcggtctac
     5461 gtagtcatgt ccggggagat aaagctggtc agaaggaata gtcatacaac ggtgctgtaa
     5521 aaggttgtcg gcgtctttgc caagcaactg cgcaatatct gtcattacta tgctcccgta
     5581 aattccgatt ggatatcggc tatggattgt cctggcccgc cttttgcggg caatcataat
     5641 cctggtcgtt acgggcgagt ttttccatct tttggatgtt ttttcagcgt tttctgttgg
     5701 ctcgattcat cagaaaaaat gttagcgcgg tcaagttttt accgcaaagg tattcaaaag
     5761 gtattattat gtggtattgt catcgcgtac cttacattac ctgtcaattt cacctacgcg
     5821 ttactgttcc tcggtatttt gcttcacggc gtaagttacg atttttacta cgttaccgct
     5881 tacatctatg tcgataaaaa agcccccgtg catatgcgta ccgctgcgca ggggctgatc
     5941 acgctctgct gccagggctt cggcagtttg ctcggctatc gtcttggcgg tgtgatgacg
     6001 gaaaagatgt tcgcttatca ggaaccggta aacggactga ctttcaactg gtccgggatg
     6061 tggactttcg gcgcggtgat gattgccatt atcgccgtgc tgttcatgat tttttcccgc
     6121 gaatccgaca acgaaattac ggctatcaag gtcgatgatc gagatattgc gttgacacaa
     6181 ggggaagtta aatgaaaaca gaacgtattc tcggtgctct ttatgggcag gcgttagggg
     6241 atgcgatggg gatgccctcc gagctttggc cacgcagccg cgttaaagca cactttggct
     6301 ggattgaccg ttttctgcct ggaccaaagg agaataacgc ggcctgttat tttaaccgcg
     6361 ccgaattcac cgacgatacc tcgatggcct gtgtctggcg gatgcgttac tggaatgtga
     6421 aggcaagatc gatccgaatc tgattgggcg taatattctc gactgggcgt tgcgtttcga
     6481 cgcctttaac aaaaacgtac taggtccgac ctcgaagatt gcgcttaacg ccattcgcga
     6541 cggtaaaccc gttgctgaac tggaaaacaa cggcgtaacc aacggcgcag cgatgcgcgt
     6601 ctcgccatta ggttgtttgc ttccagcgcg tgatgttgat tcctttattg atgatgtggc
     6661 gctggcgtcc agcccgaccc ataaatccga tctggcggtt gcaggcgcgg tagtcatcgc
     6721 atgggcgatt tctcgtgcca ttgacggaga acgctggtca gcgattgttg attcattgcc
     6781 ttcaattgcg cgacatgcac agcaaaaacg caccaccacg ttcagcgcct cactggcggc
     6841 acgtctggag attgcgctga aaattgtgcg taatgccgac ggcaccgaat ccgccagcga
     6901 acagctttac caggtcgttg gcgcaggcac cagcactatt gagtccgttc cgtgcgccat
     6961 tgcgctagtt gaactggcac aaaccgaccc aaaccgctgc gccgtcctgt gcgccaatct
     7021 tggcggcgac acagacacca taggtgctat ggcgacggcg atttgcggcg cgttgcatgg
     7081 cattaacgct gtcgatcctg cgttaaaggc ggagctggat gcggtaaatc agcttgattt
     7141 caaccgctat gccacagcgc tggagaaata tcgtcaacaa cgggaggcgg tatgagcggc
     7201 gctcgattac acacactgct gcctgaatta accacgcgtc agccggtgat ggtcgtcggc
     7261 gcggcggtca ttgacgtgat cgccgacgct tatgccctcc cctggcgtgg gtgcgatatc
     7321 gaactgaaac agcagagcgt taacgttggc ggctgcgcac tgaatattgc cgtggcgtta
     7381 aagcgcctcg gcatcgaagc gggtaatgcc ttgccgctcg gtcagggcgt gtgggcggag
     7441 attattcgca accggatggc gaaagagggg ttaatcagcc tgatcgataa cgccgaaagc
     7501 gataacggct ggtgtctggc actggttgag ccggatggcg aacgcacttt tatgtcattc
     7561 agcggtgtgg aaaatcaatg gaatcgccaa tggttggcac gattaaccgt tgcgcctggc
     7621 aacctgctct atttttccgg ttatcaactg gcctcgccct gcggcgaatt gttagtggaa
     7681 tggctggaaa agctgcaaga cgtgacgccg tttatcgatt ttggcccacg tattggcgat
     7741 atcccggacg cattactggc gcggatcatg gcctgtcgac ctttagtgtc actcaatcgt
     7801 caagaggctg agattgccgc cgaacgtttt gctttatctg cagagataac aacacttggc
     7861 aagcaatggc aggagaaatt tgccgcgccg ttgatcgttc gtctcgataa agaaggcgca
     7921 tggtatttca gcaacgacgc ttctggctgc attcgggcat ttccaacgca agttgtagac
     7981 accattgggg cgggcgacag tcatgcaggt ggcgtacttg ccgggctggc ctctggtctg
     8041 ccactggcgg atgccgtatt actgggcaat gcagtggcgt cgtgggttgt cgggcatcgg
     8101 ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc
     8161 gctgcgacag tggctaatgc tgtactcaat aggccgttgc tgttggtcaa gcgccacttg
     8221 cttgatcacc agcaccggta ttttgctatc taactgaata tgtgactgaa actcggcatc
     8281 cggcatccgg gcgctaacgc gggaacgcgt acgttgtggg taaatgtgct ggctgcggaa
     8341 atagtcataa agagaaattc cgatggcatc aacatcatga attaaatgcg ccggaaccca
     8401 cgattcctca atcgataccg cttcttcatc gacatagcga atacgcttaa gcaaaaagac
     8461 atcacttccc gcttcgaccg ccagctgctg cgcaacttct tcgggacatt tcacgacacg
     8521 cttattaacc catagcgtat cgggcttttt cccgcgcaat accacctgct gagaaaaacc
     8581 tctcgcttct ttcaacgagt attcgaagat gttgttgatc tgcgtgccgt aaccgcgcga
     8641 acgcgtcacc acgccctctt cttccagcgc ctgcatcgcc ttgcgcaccg taatgcgcga
     8701 aacgccggtt aactgactta aatcacgctc accgggcaaa atattgccat gctccagcac
     8761 cccgctgcgc acggcatttt ttaccgtttc ggcaaactta atataaagcg gcgtgttatc
     8821 cgccgctaaa atacgctcgt tcagttgggc aatcagctgg gtatgcgctt gttccatttg
     8881 tctttttcct gccagggagt ctggtgggag tatatcactg cttcttattt aaggcgtttc
     8941 tttaatccca tcaacgaatg cctgcaactc atccaccgtg ccattaaaca cattaaaatc
     9001 caccggacca ttgatgccat ctacctgtcc acggtcggaa tgctgccaga agcgccaggc
     9061 catgccgtca ttgtccggac gacgttgata atagtgagcc acccaccacg gatattcatt
     9121 gaaatagccc gccagattgg tgtgataaaa aacggctcct gagtaaataa tcggcttttt
     9181 tcccgtgcgt ttttcgacca tttttagcca ctgacttacc cgcttgcgta attcttttgc
     9241 cgataatttt ccccgttctt ctacgtccag cacggcaggg aaatcgcctt gtgagaaatc
     9301 caccgtttgc agaaataatc gcgcctgaac tgaagcggat accgacgggg agaaataatg
     9361 atacgccccg cgcagcaggc cattttcgcg gcttaattgc cagttacgcg aaaaataggg
     9421 gtccaccagc ttttcgcctt ccgtagcctt aataaaagca aactgtaagc ggatatcgtt
     9481 gtcgcgcatt tttgccaccc gctgccagtc gatacgctcc tgccagcgcg aaacatctaa
     9541 tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa gatttaaccg ggcgataacc
     9601 gtagaagtgg atgtagtctg taatctgaac tgctgtcgaa tagaaaaaat tcaccgtctg
     9661 acgcggataa attgcaacga tggaaattag cccaagagcg cataatagag cggtgagttt
     9721 ttttcgactg gtaattctta gctgcattgg tttcatccct gaatgtcagt gccaaaggct
     9781 gacaataacc aaagcagcta tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg
     9841 gaccaatacc gtgaccaact tccagcgtgt cggcctgggc taacgccgat gaaagccagc
     9901 tttttgcctc ctgtacggtg tcagcccagt ttgtatggcg cgggcgtagt gcagccaacg
     9961 ccgcagagag tgtacaacca gtgccgtggg tgtttttggt cataatgcgc ggtgcggtaa
    10021 accgttgttc accctcgcgg gtaaacagcc agtccgggct ttgctcatca tccagatgac
    10081 cacctttcat tagcactgcg ccacagccca tcgccaacag cgatcgccct tgttccagca
    10141 tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc ggcagcttcg ggcaagtttg
    10201 gcgttattaa tgaaacctgt ggcaataatc gactgcgcag cgtagcaacc gccgaaggtg
    10261 aaagcagcgg gtcgccgctt tttgccagca taacggtgtc gagtaccacg ttttggatct
    10321 gataacgttg caaccgttct gccaccgctt caacaatatc ggtttccgcc aacataccga
    10381 ttttagtggt atcgattcgc acatcgctga acaccgaatc gagctgggcg gcgacaaaat
    10441 caggctcaat gcgatacacc gactgtacgc cacgggtatt ttgcgccacc agtgcagtaa
    10501 taactgagca accataagcg ccaagtgccg agaaggtttt aagatcggcc tgaatccccg
    10561 caccaccact cggatcagta ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac
    10621 ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc
    10681 gcttctggcg actgcgcgtt ctccggcttg tttcatccag tgacaggcag atgcgacatt
    10741 ttccagcgta tcgcctggta acgcacagca ggcagcgaca accgccgata atgcacagcc
    10801 agttcctacc actttggtca ttaacggatc gccaccgtga ataccaatga tacgatgtcc
    10861 atcggtaaca taatccacct cgccagtgac cacgacgatt gcgccagttt cccgtgccag
    10921 tgtttgtgca gcgggtatcg cgttagctgc ggcgtcagtg gtatccactc cccgtccgcc
    10981 attagcaatg ccagctaatg ccatgatttc cgaagcatta ccacgtatcg ctgccggttt
    11041 aaaagataaa agttcatgac aaaaatggcg gcgataatcg agcgcaccaa ccgctactgg
    11101 atcaagcgtc cagcgtgttt gagagctttt tgcttgctca acggcagcac gcatcgcctg
    11161 tgcgcgtggc tgcgtcagtg tgccaacgtt aatcaacaag gcactggcga tagccgcaaa
    11221 ctgactggcc tcttcggttt cgataaccat cgctggcgat gcaccgagcg ccagcaaggt
    11281 attggcggta aaggtttgca ccacatcatt ggtcatgcag tgcacaagag gggaatgttg
    11341 gtgaaaaagg tgtaacgcgt gcgcagattg cgctgaactc agcaggtcga cttgcatagt
    11401 ttgctcctgc cataacgtga agaagcaatg acctggtggt ccgtgacttc cctacgctgg
    11461 cattatccag atcaggtgat acgggtattt ctcagccttc acgcagaagg gcaccccgag
    11521 tcgtttggtt gcgatgataa atcgcagagg aggatggtaa tgtccagcgc acccgttgta
    11581 aacgaggaaa aacggcagtt ttacaatcgc gttaataaat tatttgatat atgaatccag
    11641 caccttcaga acgacatcca gatcttcttc acgttttagc tcatccccct ggtgaacgat
    11701 gtgttccgtc agatgacctt taatcacttc ccgcatcaga ccgtttaccg ctccacggat
    11761 agcagcaatc tgttgtaaaa ctgcagcgca ttcgtgcggc tcgtcgagca ttttcttgag
    11821 cgccacgacc tggccctgaa tcttactggc acgcgctttc agtttctgtt tatcacggat
    11881 tgtatgagac atggcaacac ctggttaaca agaatatgaa aaatcatagc actattaatc
    11941 tactgggggg tagtatcagg tactgggggg gagtagaatc agattgccga attaatacta
    12001 agaattatta tcatgaccga atttacaact cttcttcagc aaggaaacgc ctggttcttc
    12061 atccccagcg ccatcttact tggtgcgctt catggcctgg aaccagggca ctcaaaaacg
    12121 atgatggcgg cgtttatcat cgccatcaaa ggcaccatta aacaggcggt gatgctcgga
    12181 ctggcagcaa ccatttcgca taccgcggtg gtctggttga ttgcctttgg cgggatggtg
    12241 atcagtaagc gctttactgc tcaatcagca gaaccgtggc tccagctgat ttccgcagtg
    12301 atcattattg gcaccgtgtt ctggatgttc tggcgtacct ggcgcggcga acgcaactgg
    12361 ctggagaata tgcacgggca tgattatgag catcatcacc atcatcacga acatcatcaa
    12421 gaccacgaac atcaccacga ccaaggacat catcaccatc acgaacatgg cgagtatcag
    12481 gatgcccatg cacgagccca tgccaatgac attaaacgac gctttgatgg tagagaggtc
    12541 atcaactggc aaattttgtt atttggctta accggtggcc ttatcccctg cccggcagca
    12601 attaccgtgc tgttgatatg cattcagttg aaagccctga cactgggcgc aacactggtc
    12661 gtcagtttca gcattggcct ggcgttaacg cttgtcaccg taggcgttgg cgcagcaatc
    12721 agcgttcagc aggtcgcaaa acgctggagc ggatttaaca ctctcgctaa acgcgccccc
    12781 tatttttcca gtctgttgat tggcttagtc ggtgtgtata tgggcgtaca tggcttcatg
    12841 ggcataatgc gataactcaa tcctttaggc ttgcatgtta tcttcagccc aggatgattc
    12901 ttaaatcagc tatttccgct gactctctgc tcgctaagga tgcttttagg gcatcctttc
    12961 atttacactt tttacgaaat catgggatca ctaacaaaat atcgcttgtc agttatattg
    13021 tatggcagga aagatatgcg actgatatta cagatcccca aagtggagag tttatgacca
    13081 ttaaaaataa gatgttgctg ggtgtgcttt tgctggttac cagtgccgcc tgggccgcac
    13141 cagccaccgc gggttcgacc aatacctcgg gaatttctaa gtatgagtta agtagtttca
    13201 ttgctgactt taagcatttc aaaccaggtg ataccgtccc agagatgtac cgcaccgatg
    13261 agtacaacat taagcagtgg cagttacgta acctgcccgc gcctgatgcc gggacgcact
    13321 ggacctatat gggtggcgcg tacgtgttga tcagcgacac cgacggtaaa atcattaaag
    13381 cctacgacgg tgagattttt tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa
    13441 tgcagacacc ttgttatttt ttattattag ccacttgctc gtcttgcttg ttattagtcg
    13501 tatttcacgt tgattaatgc ggttgcctcc agtgcgccag atttaacttt gtttgtatcg
    13561 tagacgtagt aactggctgt tatcggaatt gaaaccgttg tatctgtaag ttttgacttt
    13621 gccgcaattt cttttaaatt tgtgtatcct ttactgctgt tagtgatatt taaaagtgtg
    13681 actggcgaac cattactttc aagaataaat cctacgcatt atcttccgca cctttcagta
    13741 ttgtgtcgct gttggttaac aagtcagaac ttgataaata cacctgcatt ttccgtgatg
    13801 tttgattttg ttgacctaat aaatttttac aacgcagaat aatatccctt tttttactat
    13861 ttattgttgc tttgttacct gtggctggta attgtgaaac gggaatactg tctaaagtaa
    13921 ttaataaatc gtcctgtttg caggttgtgg ttatcgggtt gtatgacaat gtcagattga
    13981 aggaatagac acctgcattt tgtgccaatg catttctgta gctatttaca catgcatccc
    14041 atgacttgcc acctaataaa cacagtagaa attgtactgc ggcactcccg cgtgtcgatg
    14101 atgaagcact ggataatgtc gcaacaccat taatcatcac tgaaccaccg ccccccgcag
    14161 aaatattcac ttttgcatta ctggccgcag gtgtcaccgt taccgaataa ccactggtga
    14221 aactgctctg attatttgac aacgtgatgt tgtttttatt ggtccattca aaatgtaaat
    14281 ttaatttaat ggtatcgtta taaagtccaa caataatatc cgatgggctc ataggggtga
    14341 tagtatttgt actcacgcat gacgaatcat taaaattatg ctcatatgtc gctatttgtg
    14401 tttgagtaag tgcaaaattc ccctgaacaa ccaaattgtg ctgaacagat aactcactat
    14461 aacatgccgc atagctcaca ggcataacca tcatcattaa tataaatagc attatgcgaa
    14521 tttccataga accacctgac tattactggc aaatataatt tctactttca tcaatctctt
    14581 taccgaaggt gattgtgcat gaaagtcctt gttgcttatc aattgctaca ttaaccgatg
    14641 gcggtatttc attggtgcga ataaataact ggcttccctg gccgacaacg ccaatattat
    14701 gaccatggat atcattgact tcataaccaa acgttaatgg ttgaccatcc gctcttaacg
    14761 cttttataaa ccagggcttg cgctgatcgg tatcaaaatt aaccagtaca accgcgccgc
    14821 gataaggggc ggcaattttc cggttgccac gtaattctgc ttcgctatcg ctttgcgaca
    14881 catccagcat caggtgattt tcccgataag gtgtcattcc gtcgtatacc accactccat
    14941 tacggtttgt tgtgcgatat ttttgcccat tgacataagc atctttaatt cctggcgcat
    15001 tcatcacagc aaacgtttcg gaaagacggt tcgccagatt aacgccaccc gaccaggcga
    15061 caatgccccc tgaaacactg gctccagtct gtcgataagt actcgactga ctataactgc
    15121 cattcactgt cgcaaccggc gcgttccagg tcaaattcgc cccagctgtc gtttcatttc
    15181 cctgatgttg atgactcagg ttgacaccat aattgaactg atcccgactc cctactgttc
    15241 ctgataatcc cgtattattt gaggcaaaac cctgatcatc aaacgtcgtt gagttagaca
    15301 tatatatttg ccgacgaggc gtcgtaacgt catcacccca atcacaggga attgatataa
    15361 aaatattaaa acgtttctct tcatgatgat tctcgccata cgcctggctt gccgcgaggg
    15421 tatagcttat ccgccgccag ttattggaat aactcaactg ataatcctta ctgctgccgc
    15481 tacgccccca gtaatctcgc cataacgtac ttaaggacac agaaccccag ccttctggca
    15541 atgactggct catattggca gaaaagctat ttttacggcc aaaatcgttc tggtaataat
    15601 cggcaatgtc atagatatcg ttttcatcac ggcgataatt atctttattg tttgcccaaa
    15661 cgtgatcgtt aaatgtccgg taatcacgcg acgaataacg ccaggccgcc agaccaaaac
    15721 gcgtcgacgt ttggctcaca aatttgttgt aggcaatttg ataactttgc ccgtcaaaca
    15781 catcgccgtt gtcttgttta ctatgcgact tcgtggcatc gacggaaatt gcgccaatgc
    15841 gcgtgttcca acccgttccg agggtaaacg catagtaatt attagcaacc atcgtgccgc
    15901 catacagcgt caataagtta ttaaaaccat actgataacc tgcctggaca aaatcacttt
    15961 gcttgctcgc cccttcaata tggctacggc ccgccgcaaa atcatatttc gacacgccgg
    16021 gttgcagcat atttggcacc gctgcataag gcaccagata ggtggttacc gagccgtccg
    16081 cctctttcac gctaacatca agatctgctc caccaccggc caactgcaaa tctgtaatcg
    16141 caaacgggcc aggaggaacc tctttctgat aaaccacaaa accattctgt tcaatagtta
    16201 ccaacgcgtt actctgagca atcccctgca cccgtggcgt aaaattttgt ttcgagttag
    16261 gcaacatctg catatcacga aacaaccgca caccgctgaa gcgaacagaa tcaaaaatat
    16321 cgcttgatgt gtacatatca cccacgcgaa gcgtgccgag aaattgggca aatccacgtt
    16381 ccagatacag ggtattgctt ttccacaccc ctggattgtt atttgtttta ctgaaactgg
    16441 catcagaatg cagttgccac tccagtaaat ttaacccgct gttaaaacgt acatatgtac
    16501 tcttgttgtt acccgacgct ttatagtcgc tgtaatactg actcacataa taagaagtat
    16561 aaaacgcatt aataccccgc tcccagtttt ccggtggaac atagccactt tccagttctt
    16621 ccacccaagc ctgcgggaca ctgaaatcga gacgaaaaac cccgatatcc cagctatagc
    16681 tcccaccctg aacaagttgc tcaaatgtta aacattgctt accgctggcg aagttatcgc
    16741 tattaatgcc taaccgcttg ataacttctc ttgataaaca tgtttcttgc gggttgtctt
    16801 taacaataat ctcatatttc ccgcgccatt gcttattgac ataaatatcg atgtcatact
    16861 gccccggtaa gggttgatta tcatcaagac gaatatttgc aacctgctgg tctttcattc
    16921 cacctatcat aaaatgggta tcaaaggttt cttcagctgc ataagcttca atgccgagca
    16981 ataacgctac gattgctgat gcaagtgggg tcattctcaa cataggcagc tcctgcaatt
    17041 aaattttgtc actaatatag ttgccgtggt cattgataat ggtcagatac cagttatttg
    17101 cattattact tttgatatta acactctgac tttctaaggg ggcaatcata atagtttcat
    17161 aattgacttt aacattatta gctttgacat ccgaaatcgt cacccaatta gctgagtcat
    17221 tttttatcac caatccattg ccactgcgat ttaccagtaa ctttttaaag gtcgctttat
    17281 ttaccggagc aatacccgct ggccggtaaa acaacttaat tctgttttgc atcgcaaact
    17341 tcagtgcatt cttgccttct tgctctggac tatttggtgg aatatccaga acattcagat
    17401 aaaaaatgct ttctttatta gtgggcagtt tattcggcat aattttgatt tttacttgct
    17461 gccctgaatt tgcccctatt tttgccactg gtggcgttaa catgaaagga acctgaattt
    17521 tttctggtgg taacgacgta tcgccatcat caatccacgc ctgcagcagc gaagaacgat
    17581 ttccctggtt catcaactgc accataactt ctttattttc tgccgggtaa ataatgcgcg
    17641 tcccgtagat aacaattccg gcatgtgcag gaaggaccat agaaaaaatg agtaaagata
    17701 ataatccttt catcttaatg tctcttaaat taaaaagccg ccatggggat agcggccatg
    17761 tttattacct ggagaaactt gcaattttat ttattaatcc tgcgctacac gcataaccag
    17821 agaagataac acatcacctg attgcactga gttatctgtc agtttcaggt attttgcttt
    17881 aaacggaata gcatattgac cttctgctgt tttataactc tcaatgttaa aaggtgcaga
    17941 aacatctagg acatcgctgt tatcacctaa atagagatag taacccacgg tatcgttgct
    18001 actggctatc gccttatcgt taccggttat tttgtcgttt gtgatcaacg agagttttac
    18061 accagcaact tctttcgggc agctaatttt taacatgctg ctggttacac cgactgcatc
    18121 atcataggta gaatatttag ctttaccttc aaagacagtg gttggtacag tcggcatatt
    18181 aatacttact gaatcaccac ccgcttcgaa tttacacgga gattctgcaa tatttccttt
    18241 aatggttaat gttccggtat caacgtctgc agcaaatact ccagcgctca taaaaaaaga
    18301 agaaaagaca gcggcagcaa taattgaacg tttcataata tgttccttaa taaaatttat
    18361 ttttggttgt taagagtctg tatctcttgg aaacaaattt ctcacatcaa aaaagctgcc
    18421 gcaatgtaaa catattgttt tattgattta aaacaaatta accaatgcaa tataaataca
    18481 acaaatactt aagaatatta aatatttatc acatctatta attaatgtag atttatgtaa
    18541 atggtattgt tgatatcaat aaaaaagacg aaatcattta tataaatgat ttcgtctttc
    18601 taaatgcaat taaaatcagg ttgccaaatt aatcgtagcg atttaacacg attcgacgtt
    18661 tgctatcgct acctgcaaca accacttctg tcgtaacacc tttgacgggc gaagaaacct
    18721 gatgacgggt gtcaacatta tacgttaact gaccacaatg ctgttgtggt tgactattaa
    18781 ccatagccat actgcatgtt gggggactga tcctgccatt aatcgttaat gtggcagtct
    18841 ctgaagagac tgcgggagaa gccaatccat aggcaagaaa aataattcct gacagccaat
    18901 acttattcat tgaacgttat ccctgtagta aaggttatgc ctggatagaa tgagtgcata
    18961 acaaactata gctgtacatc cactacacag cacgaaggat gataatgaag cattgcctgt
    19021 acgatcactc gactttgtag agtttcggac gaaggtccgc agaatattcg cagtattaaa
    19081 taagtgttca ttaatgcggc aaaaacaaat acaccgcatc aatataacat ctctgcgatg
    19141 tatgaagaat aaccagaatg gttaataggc gttagaccgc gcggaaggaa atctcgccag
    19201 gaatgacttc accctgccag tagagctgag ctgcaacgcg gtcagccagc tggcggtaga
    19261 tagcggtaaa ttcgctctcc ggacggctga taaccgttgg cgtgccttta tcgagatcct
    19321 cacgtaagga gatgtgtaac ggcatctgcc ccagcagctg tgtatggtat ttttcagcca
    19381 gtttctctgc accaccggta ccgaaaatcg gttcatggtg accacagtta ctgcaaatat
    19441 gcacactcat gttctcgaca ataccgagca ccggaacctc gactttctcg aacatcacaa
    19501 tgcctttttt cgcatcgatc aacgcgatgt cctgcggggt ggtgaccacg actgcaccag
    19561 ttactggaat gttttgcgcc agcgtcaatt gaatgtcacc ggtgcctggc ggcatatcaa
    19621 gcaccagata gtcgagatcc ggccacaagg tttcctgcaa catctgcatt aacgccttgc
    19681 tcgccatcgg tccacgccac accatcgcat tgtcgtcagt gaccagataa ccaattgaat
    19741 tggttgccag gccatgagac ataatcggtg ccatgtgggt accgtccggc gaagttggac
    19801 gctgattttc tgcgcccagc atggtgggaa ttgatgggcc gtagatatcg gcgtccagaa
    19861 tgccgacttt cgccccttcc gccgccagtg ccagcgccag gtttaccgcc gtggacgatt
    19921 tacccacgcc gcctttaccg gaactcaccg cgataatgtt cttcacaccg ttaattcccg
    19981 gctgattctt cacgcgtttc agagtggcta tgttatgcga cagtttccag tcgatagctt
    20041 tcgccccggt gatgcgcagc agttcggcgc tacattgctc ttttaactct tcgaacgcgc
    20101 tatgccagac aaacggcata accagttcca catgcaacgt gtcgtccatc caggcgacat
    20161 ggtgcaacgc tttcagcgtg gtgaggttat gcttcagggt tgggtgctga aaattggcca
    20221 gtgtcccggc gaccattgcg cgcaaggctt ctggtgattt ggcctgggat tgttcgttca
    20281 tcccgactcc ttttttgtat agataaacca tcagctgata gtttacctga agaatataga
    20341 gaagtactta cttaacattt tcccatttgg tactatctaa ccccttttca ctattaagaa
    20401 gtaatgccta ctatgactca agtcgcgaag aaaattctgg tgacgtgcgc actgccgtac
    20461 gctaacggct caatccacct cggccatatg ctggagcaca tccaggctga tgtctgggtc
    20521 cgttaccagc gaatgcgcgg ccacgaggtc aacttcatct gcgccgacga tgcccacggt
    20581 acaccgatca tgctgaaagc tcagcagctc ggtatcaccc cggagcagat gattggcgaa
    20641 atgagtcagg agcatcagac tgatttcgca ggctttaaca tcagctatga caactatcac
    20701 tcgacgcaca gcgaagagaa ccgccagttg tcagaactta tctactctcg cctgaaagaa
    20761 aacggtttta ttaaaaaccg caccatctct cagctgtacg atccggaaaa aggcatgttc
    20821 ctgccggacc gttttgtgaa aggcacctgc ccgaaatgta aatccccgga tcaatacggc
    20881 gataactgcg aagtctgcgg cgcgacctac agcccgactg aattgatcga gccgaaatcg
    20941 gtggtttctg gtgctacacc agtgatgcgt gattctgaac acttcttctt tgatctgccc
    21001 tctttcagcg aaatgctgca ggcatggacc cgcagcggtg cgttgcagga gcaggtggca
    21061 aacaaaatgc aggagtggtt tgaatccggc ctgcaacagt gggatatctc ccgcgacgcg
    21121 ccatactttg gttttgaaat tccgaacgcg ccgggcaaat atttctacgt ctggctggac
    21181 gcaccgattg gctacatggg ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta
    21241 agcttcgatg aatactggaa gaaagactcc accgccgagc tgtaccactt catcggtaaa
    21301 gatatcgttt acttccacag cctgttctgg cctgccatgc tggaaggcag caacttccgc
    21361 aagccgacca acctgtttgt tcatggctat gtgacggtga acggcgcgaa gatgtccaag
    21421 tctcgcggca cctttattaa agccagcacc tggctgaatc attttgacgc tgacagcctg
    21481 cgttactact acactgcgaa actctcttcg cgcattgatg atatcgatct caacctggaa
    21541 gatttcgttc agcgtgtgaa tgccgatatc gtgaacaaag tggttaacct ggcctcccgt
    21601 aatgcgggct ttatcaacaa gcgttttgac ggtgtgctgg caagcgagct ggctgaccct
    21661 gagctgtaca aaacctttac cgatgccgct gaagtgattg gtgaagcgtg ggaaagccgt
    21721 gaatttggta aagcagtgcg cgaaatcatg gcactggctg acctggctaa ccgctatgtc
    21781 gacgagcagg ctccgtgggt agtcgcaaaa caggaaggtc gcgatgctga cctgcaggcg
    21841 atttgctcaa tgggcatcaa cctgttccgc gtgctgatga cttacctgaa gccggtactg
    21901 ccgaaactga ccgagcgtgc agaagccttc ctcaatacgg aactgacctg ggatggtatc
    21961 cagcaaccgc tgctgggcca caaagtgaat ccgttcaagg cgctgtataa ccgcatcgat
    22021 atgaaacagg ttgaagctct ggtggaagcc tctaaagaag aagtgaaagc cactaccgcg
    22081 ccagttactg gcccactggc agatgatccg attcaggaaa ccatcacctt tgacgacttc
    22141 gccaaagttg acctgcgcgt ggcgctgatc gaaaacgcag agtttgttga aggttctgac
    22201 aaactgctgc gcctgacgct ggatctcggc ggtgaaaaac gcaatgtctt ctccggtatt
    22261 cgttctgctt acccggatcc gcaggcactg attggtcgtc acaccattat ggtggctaac
    22321 ctggcaccac gtaaaatgcg cttcggtatc tctgaaggca tggtgatggc tgccggtcct
    22381 ggcgggaaag atattttcct gctaagcccg gatgccggtg ctaaaccggg tcatcaggtg
    22441 aaataatccc ccttcaaggc gctgcatcga cagcgccttt tctttataaa ttcctaaagt
    22501 tgttttcttg cgattttgtc tctctctaac ccgtataaat actggtagca tctgcattca
    22561 actggataaa attacaggga tgcagaatga gacactttat ctatcaggac gaaaaatcac
    22621 ataaattctg gacggtggag caacagggaa acgagttgca tatcagttgg ggaaaagttg
    22681 gcaccaaagg gcaaagtcag ataaaaagtt tttcagatgc tgcggcggcg gaaaaagcgg
    22741 aactgaagct gattgcggag aaggtgaaga aggggtatga
//
LOCUS       DADLTI010000056        22320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLTI010000056 DADLTI010000000
VERSION     DADLTI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.056"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JN755_001948"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JN755_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7380753.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JN755_001949"
     CDS             831..971
                     /locus_tag="JN755_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380754.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JN755_001950"
     CDS             985..1857
                     /locus_tag="JN755_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7380755.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JN755_001951"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JN755_001951"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7380756.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JN755_001952"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JN755_001952"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7380757.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JN755_001953"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JN755_001953"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY7380758.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JN755_001954"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JN755_001954"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY7380759.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JN755_001955"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JN755_001955"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7380760.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JN755_001956"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JN755_001956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7380761.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JN755_001957"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JN755_001957"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7380762.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JN755_001958"
     CDS             complement(14044..15384)
                     /locus_tag="JN755_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7380763.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JN755_001959"
     CDS             complement(15386..16738)
                     /locus_tag="JN755_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380764.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JN755_001960"
     CDS             complement(17172..17621)
                     /locus_tag="JN755_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7380765.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JN755_001961"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JN755_001961"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7380766.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JN755_001962"
     CDS             complement(18421..18750)
                     /locus_tag="JN755_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7380767.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JN755_001963"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JN755_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7380768.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JN755_001964"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JN755_001964"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7380769.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JN755_001965"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JN755_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7380770.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5510 a         5917 c         5294 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctt aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctccc
//
LOCUS       DADLTI010000057        22113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLTI010000057 DADLTI010000000
VERSION     DADLTI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.057"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            270..2531
                     /gene="metE"
                     /locus_tag="JN755_001966"
     CDS             270..2531
                     /gene="metE"
                     /locus_tag="JN755_001966"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7380771.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(2570..3385)
                     /locus_tag="JN755_001967"
     CDS             complement(2570..3385)
                     /locus_tag="JN755_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7380772.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            3647..4408
                     /gene="udp"
                     /locus_tag="JN755_001968"
     CDS             3647..4408
                     /gene="udp"
                     /locus_tag="JN755_001968"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7380773.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            4549..5976
                     /gene="rmuC"
                     /locus_tag="JN755_001969"
     CDS             4549..5976
                     /gene="rmuC"
                     /locus_tag="JN755_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7380774.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            6071..6826
                     /gene="ubiE"
                     /locus_tag="JN755_001970"
     CDS             6071..6826
                     /gene="ubiE"
                     /locus_tag="JN755_001970"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7380775.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            6840..7445
                     /gene="ubiJ"
                     /locus_tag="JN755_001971"
     CDS             6840..7445
                     /gene="ubiJ"
                     /locus_tag="JN755_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7380776.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            7442..9082
                     /gene="ubiB"
                     /locus_tag="JN755_001972"
     CDS             7442..9082
                     /gene="ubiB"
                     /locus_tag="JN755_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7380777.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            9161..9430
                     /gene="tatA"
                     /locus_tag="JN755_001973"
     CDS             9161..9430
                     /gene="tatA"
                     /locus_tag="JN755_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7380778.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            9434..9949
                     /gene="tatB"
                     /locus_tag="JN755_001974"
     CDS             9434..9949
                     /gene="tatB"
                     /locus_tag="JN755_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7380779.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            9952..10728
                     /gene="tatC"
                     /locus_tag="JN755_001975"
     CDS             9952..10728
                     /gene="tatC"
                     /locus_tag="JN755_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7380780.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            10770..11552
                     /gene="tatD"
                     /locus_tag="JN755_001976"
     CDS             10770..11552
                     /gene="tatD"
                     /locus_tag="JN755_001976"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7380781.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11549..12037)
                     /gene="rfaH"
                     /locus_tag="JN755_001977"
     CDS             complement(11549..12037)
                     /gene="rfaH"
                     /locus_tag="JN755_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7380782.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            12204..13697
                     /gene="ubiD"
                     /locus_tag="JN755_001978"
     CDS             12204..13697
                     /gene="ubiD"
                     /locus_tag="JN755_001978"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7380783.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            13743..14444
                     /gene="fre"
                     /locus_tag="JN755_001979"
     CDS             13743..14444
                     /gene="fre"
                     /locus_tag="JN755_001979"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7380784.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(14537..15700)
                     /gene="fadA"
                     /locus_tag="JN755_001980"
     CDS             complement(14537..15700)
                     /gene="fadA"
                     /locus_tag="JN755_001980"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7380785.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(15710..17899)
                     /gene="fadB"
                     /locus_tag="JN755_001981"
     CDS             complement(15710..17899)
                     /gene="fadB"
                     /locus_tag="JN755_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7380786.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            18089..19420
                     /gene="pepQ"
                     /locus_tag="JN755_001982"
     CDS             18089..19420
                     /gene="pepQ"
                     /locus_tag="JN755_001982"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7380787.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            19420..20034
                     /locus_tag="JN755_001983"
     CDS             19420..20034
                     /locus_tag="JN755_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7380788.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            20073..21524
                     /gene="trkH"
                     /locus_tag="JN755_001984"
     CDS             20073..21524
                     /gene="trkH"
                     /locus_tag="JN755_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7380789.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            21536..22081
                     /gene="hemG"
                     /locus_tag="JN755_001985"
     CDS             21536..22081
                     /gene="hemG"
                     /locus_tag="JN755_001985"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7380790.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5119 a         5619 c         6101 g         5274 t
ORIGIN      
        1 cttgtagcgt tttcaggtgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata
       61 atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc
      121 taaaatcctt cgtcttttaa atttatggtg cgttggctgc gtttctccac cccggtcact
      181 tacttcagta agctcccggc gatgaataaa cttgccgcct tccctaaatt caaaatccat
      241 aggatttaca tataattaga ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc
      301 ctcgcgttgg cctgcgtcgc gagctgaaaa aagtgcaaga aagttattgg gcggggaact
      361 ccacgcgtga agaactgctg acagtagggc gtgaactgcg tgctcgtcac tgggatcaac
      421 aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt tgcctggtac gatcatgtac
      481 tgaccaccag tttgctgctg ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg
      541 tagatatcga caccctgttc cgtattggtc gtggacgtgc gccgactggc gaacctgcgg
      601 cggcagcgga aatgaccaaa tggtttaaca ccaactatca ctacatggtg ccggagttcg
      661 ttaaaggcca acagttcaaa ctgacctgga cgcagctgct ggaggaagtg gacgaggcgc
      721 tggcgctgag ccacaacgtt aagcctgtgc tgctggggcc ggttacttac ctgtggctgg
      781 ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct gaacgacatt ctgccggttt
      841 atcagcaagt gctggcagaa ctggcgaaac gcggcatcga gtgggtacag attgatgaac
      901 ccgcgctggt actggaacta ccacaggcgt ggctggacgc atacaaaccc gcttacgacg
      961 cgctccaggg acaggtgaaa ctgctgctga ccacctattt tgaaggcgta acgccaaatc
     1021 tcgacacgat tactgcgctg cctgttcagg ggctgcatgt ggaccttgta catggtaaag
     1081 atgacgttgt tgaactgcac aagcgcctgc cttctgactg gctgctgtct gcgggtctaa
     1141 tcaatggtcg taacgtctgg cgcgccgatc ttactgagaa atatgcgcaa attaaggaca
     1201 ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc gttgctgcac agccccatcg
     1261 acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac
     1321 aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc
     1381 tggcagagtg gagcgcccca attcaggcgc gtcgtcactc tactcgtgta cataatccgg
     1441 cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg
     1501 aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga
     1561 ttggttcctt cccgcaaacc acggaaattc gtaccctgcg tctggatttc aaaaagggca
     1621 atctcgacgc caacaactac cgcacgggca ttgcggaaca tatcaagcag gccattgttg
     1681 agcaggaacg tttgggactg gatgtgctgg tacatggcga ggccgagcgt aatgacatgg
     1741 tggaatactt tggcgagcac ctcgacggat ttgtctttac gcaaaacggt tgggtacaga
     1801 gctacggttc ccgctgcgtg aagccaccga ttgtcattgg tgacattagc cgcccggcac
     1861 cgattaccgt ggagtgggcg aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga
     1921 tgctgacggg gccggtgacc atactctgct ggtcgttccc gcgtgaagat gtcagccgtg
     1981 aaaccatcgc caaacagatt gcgctggcgc tgcgtgatga agtggccgat ctggaagccg
     2041 ctggaattgg catcatccag attgacgaac cggcgctgcg cgaaggttta ccgctgcgtc
     2101 gtagcgactg ggatgcgtat ctccagtggg gcgtagaggc cttccgtatc aacgccgccg
     2161 tggcgaaaga tgacacacaa atccacactc acatgtgtta ttgcgagttc aacgacatca
     2221 tggattcgat tgcggcgctg gacgcagacg tcatcaccat cgaaacctcg cgttccgaca
     2281 tggagttgct ggagtcgttt gaagagtttg attatccaaa tgaaatcggt cctggcgtct
     2341 atgacattca ctcgccaaac gtaccgagcg tggaatggat tgaagccttg ctgaagaaag
     2401 cggcaaaacg cattccggca gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc
     2461 gcggctggcc agaaacccgc gcggcactgg cgaacatggt gcaggcggcg cagaatttgc
     2521 gtcgggggta aatccaaacc gggtggtaat accacccggt cttttctcat tacagcgact
     2581 tcttcccacc atactgctta aaccattcca gcatacgctg ccagccatct ttcgcagatt
     2641 cggcatgata gctcgggcga taatcagcgt tgaatgcatg cccggcatcc gggtacacga
     2701 taatctctgc tttcgcatta gcagcccgca gcgcctggcg catcgtttca acgctctcct
     2761 gcggaatgct gttatcctga ccaccatata agccgagaac cggtgcgtta agatcggttg
     2821 cgatatcaac aggttgtttc ggtgaattca gcgacttgtc gcccgtcagt ttgccgtacc
     2881 acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac
     2941 cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac
     3001 tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc
     3061 taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag
     3121 gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg atatgttcat
     3181 gcacgccaaa aatttcctgc actacaatga ccactggcag tgggccatcg ctttgctttg
     3241 gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca
     3301 caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa
     3361 atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa
     3421 tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca
     3481 gtcctgcaga atgaagagtg atttatgtga tatgaatcac ttttggtggg taaatttatg
     3541 caacgcattt gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt
     3601 gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga
     3661 tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc
     3721 tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc
     3781 atctcaccgc gaattcacta cctggcgtgc agagctggat ggtaaacctg ttatcgtctg
     3841 ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg
     3901 cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg
     3961 tgatgtcctg gttaccacgg cgtctgtccg tctggatggc gcgagcctgc acttcgcacc
     4021 gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa
     4081 atctattggc gcgacaactc acgttggcgt gacagtttct tctgatacct tctacccagg
     4141 tcaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga
     4201 agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaactc tgctgaccat
     4261 gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt gttatcgtta accgcaccca
     4321 gcaagagatc ccgaatgctg agacgatgaa acaaactgaa agccatgcgg tgaaaatcgt
     4381 ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc
     4441 ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg gacgtttgta
     4501 cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca
     4561 atcatggttt acgcagttat tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt
     4621 tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat ggtcgcggag
     4681 ttaagcgcgg caaaacaaca aattacccaa agcgagcact ggcgtgcaga gtgtgaattg
     4741 ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc cgatctgcgt
     4801 gaagtaacca cgcggatgga agccgcacag caacatgctg acgataaaat tcgccagatg
     4861 attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg tatttttgag
     4921 cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg
     4981 ctacgtgaac aactggacgg tttctgccgt caggttcagg acagcttcgg taaagaagca
     5041 caagaacgcc ataccctgac ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg
     5101 gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac
     5161 tggggcgagg tagtattgac gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa
     5221 tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc
     5281 gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct ggtcgcctat
     5341 gaacgctatt ttaacgccga agacgactac acccgcgaaa gcgcgctaca ggaacatatc
     5401 gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca gctgccgggg
     5461 ctgcgaactc tggattacgt gctgatgttt attcccgttg aacccgcttt tttactggcg
     5521 cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat gctggttagc
     5581 ccgactacgc tgctggtggc gttgcgtact attgccaacc tgtggcgtta tgagcatcaa
     5641 agccgtaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg
     5701 ttcatcgatg acatgtccgc gattggtcaa agtctcgaca aagcgcagga taactaccgc
     5761 caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt
     5821 cgcggtttag gagtagaaat taaacgcgag attaatccgg atttggctga acaggcggtg
     5881 agccaggatg aagagtatcg acttcggtcg gttccggggc agccgaatga tgaagcttat
     5941 caacgcgatg atgaatataa tcagcagtcg tgctagccca ttaggggtag ttaagccggg
     6001 tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt tttgatgagc
     6061 aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc agaccgtcgc
     6121 gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat caaaatacga
     6181 tgtcatgaat gatttgatgt catttggtat tcatcgtttg tggaagcgat tcacgattga
     6241 ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca ccggcgacct
     6301 gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa gtggtccttg ctgatatcaa
     6361 tgaatccatg ctcaaaatgg gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt
     6421 tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct ttgattgcat
     6481 caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc gttcaatgta
     6541 tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc caattatcga
     6601 gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta ttggctcact
     6661 ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta tgcatcccga
     6721 tcaggatacc ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg actactacaa
     6781 tctgacggca ggggttgtgg cgctgcatcg tggttataag ttctgacagg agaccggaaa
     6841 tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc
     6901 gctcacccgc gctgaaaaca gcccgctcgc gtctgctggg taaagtattg cgcgtggagg
     6961 taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtt gatgtactgg
     7021 gcgaatgggc aggcgatgct gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac
     7081 ttcgcgatcg ccagcagctt accgcactga ttcgcagtgg tgagctggaa gtgcagggcg
     7141 atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg
     7201 aactgctggc cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg
     7261 gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg gcggaagcca
     7321 ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt gcggaagaga
     7381 cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta
     7441 aatgacgcct ggtgaagtac ggcgcctata tttcatcatt cgcacttttt taagctacgg
     7501 acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat ggcgatactc
     7561 attattctgg atgccaaatc ggcataaaga caaactttta ggtgagcgac tacgactggc
     7621 cctgcaagaa ctggggccgg tttggatcaa gttcgggcaa atgttatcaa cccgccgcga
     7681 tctttttcca ccgcatattg ccgatcagct ggcgttattg caggacaaag ttgctccgtt
     7741 tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc cggtagaagc
     7801 gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc aggttcatac
     7861 cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt
     7921 gccggtcatt aaagcggatc tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt
     7981 gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg aaaagacctt
     8041 gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt
     8101 tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta gtgaagggat
     8161 gatggtgatg gagcgcattt acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa
     8221 cggcactaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt
     8281 tcgcgacagc tttttccatg ccgatatgca ccctggcaac atcttcgtaa gctatgaaca
     8341 cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc taaacaaaga
     8401 agataaacgc tatctggcag aaaactttat cgccttcttt aatcgcgact atcgcaaagt
     8461 ggcagagcta cacgtcgatt ctggctgggt gccaccagat accaacgttg aagagttcga
     8521 atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt
     8581 tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc
     8641 gcaactggtg ttactccaga aaaccctgct ctatgtcgaa ggggtaggac gccagcttta
     8701 tccgcagctc gatttatgga aaacagcgaa gcctttcctg gagtcgtgga ttaaagatca
     8761 ggtcggtatt cctgccctgg tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa
     8821 aatgccagaa ctgcctgaac tggtttacga cagtttgcgc cagggcaagt atttacagca
     8881 cagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg
     8941 ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt tggtcagccg
     9001 acctgagtgg gggctgatgc ccggctggtt aatggcaggt ggtctgatcg cctggtttgt
     9061 cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt aacgtataat
     9121 gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta tcagtatttg
     9181 gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa agctcggctc
     9241 catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga
     9301 accaaagcag gataaaacca gtcaggatgc tgattttact gcgaaaacta tctccgataa
     9361 gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc acgataaaga
     9421 gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg ctattggtgt tcatcatcgg
     9481 cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag cgggctggat
     9541 tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg agttaaaact
     9601 ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc
     9661 cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga agcgttccta
     9721 cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata acccggtggt
     9781 gaaagataat gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag
     9841 ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc
     9901 tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa catgtctgta
     9961 gaagatactc aaccgcttat cacgcatctg attgagctgc gtaagcgtct gctgaactgc
    10021 attatcgcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga catctatcac
    10081 ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat cgccaccgac
    10141 gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct gattctgtca
    10201 gcgccggtga ttctctatca ggtgtgggca tttatcgccc cagcgctgta taagcatgaa
    10261 cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat cggcatggca
    10321 ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac cgcgccggaa
    10381 ggggtgcagg tatccaccga catcgccagc tatttaagct tcgttatggc gctgtttatg
    10441 gcgtttggtg tctcctttga agtgccggtg gcaattgtgc tgctgtgctg gatggggatt
    10501 acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc
    10561 gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc tattggcgat cccgatgtac
    10621 tgtctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg
    10681 gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat tcaaccgccc
    10741 gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat ttgaccagtt
    10801 cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg
    10861 ggctactcat caccggcact aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc
    10921 agtattcgtc ctgttggtca acggcgggcg ttcatcctca cgacagcagc cagtggcaag
    10981 ctgcgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg gcgattggtg
    11041 aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa cgcgcttttg
    11101 ttgctcagct acgcattgcc gcagaattaa acatgccggt atttatgcac tgtcgcgatg
    11161 cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct ggtgcggttc
    11221 ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata
    11281 tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc
    11341 cgttgattcc ggcggagaaa ttgctgatcg aaacagatgc gccgtatctg ctcccgcgcg
    11401 atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc catattttgc
    11461 aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc acggatgcca
    11521 atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg ttcttcacac
    11581 tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc tcaccatcgg
    11641 gttcggtgaa aatggcctga aagccttcga acgcgccttc ggtaataatc accttatcac
    11701 ccggataagg gggggccgga tcgacaatgt ctttcggttt atataccgat agctgatgaa
    11761 tcaccgccga tgggactatc gctggcgacg cgccaaagcg cacaaagtgg ctgacgccac
    11821 gcgtagcgct gatggtcgtg gtatgaatca cttctggatc aaattccaca aacaggtagt
    11881 tagggaataa cggttcactg actgcggtac gtttaccccg tacgattttt tccagggtga
    11941 tcatcggagc gaggcaatta accgcctgtc tctcaaggtg ttcctgggca cgttgaagtt
    12001 gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc cgtttaatcg
    12061 gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt ttgcgttata
    12121 ccgtcagagt tcacgctaat ttaacaaatt tacagcattg caaagatgaa cgccgtataa
    12181 tgggcgcaga ttaagaggct acaatggacg ccatgaaata taacgattta cgcgacttcc
    12241 tgacgctgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg gatccgcatc
    12301 tggaaatcac tgaaattgct gaccgcactc tgcgtgctgg tgggcctgcg ctgttgttcg
    12361 aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg
    12421 tggcgatggg catggggcag gaagatgttt cggcgctgcg tgaagttggt aaattattgg
    12481 cgtttctgaa agagccggag ccgccaaaag gtttccgcga cctgtttgat aaactgccgc
    12541 agtttaagca ggtgttaaac atgccgacaa agcgactgcg tggtgcgccc tgccaacaaa
    12601 aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc tgctggccgg
    12661 aagatgccgc gccgctgatt acctgggggc tgaccgtaac gcgcggcccg cataaagagc
    12721 ggcagaatct gggcatttat cgccagcagc tgattggtaa aaacaaactg attatgcgct
    12781 ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg catccgggcg
    12841 aacgtttccc ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc ggtgcagtca
    12901 cccccgttcc ggatacgctt tcagagtatg cgtttgccgg attgctacgc ggcaccaaaa
    12961 ccgaagtagt aaagtgtatt tccaatgacc tcgaagtgcc agccagtgcc gaaatcgtcc
    13021 tggaagggta tatcgatcct ggtgagatgg cgccggaagg gccgtatggt gaccacacag
    13081 gttactataa tgaagtcgat agtttcccgg tgtttaccgt gacgcatatt acccagcgtg
    13141 aagatgcgat ttaccattcc acctataccg ggcgtccgcc agatgagccc gcggtactgg
    13201 gcgtggcgtt gaacgaagtg tttgtaccga ttctgcaaaa acagttcccg gaaattgtcg
    13261 atttttacct gccgccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac
    13321 agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta
    13381 tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg
    13441 tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg gtagaaaata
    13501 cgcctattga ttatctggat tttgcctcgc ctgtctctgg gctgggttca aaaatggggc
    13561 tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt cccatcaaaa
    13621 aagatccaga tgttgtcacg tatattgacg ccatctggga tgaactggct atttttaaca
    13681 acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga cagagaaagc
    13741 gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat accgtatatc
    13801 gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc tggtcagtat ttgatggtag
    13861 tgatggatga gcgcgacaaa cgtccgttct caatggcctc gacgccggat gaaaaagggt
    13921 ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc
    13981 gcatcctcaa agatcatcaa atcgtggtcg acattcccca cggagaagcg tggctgcgcg
    14041 atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct tatgcccgct
    14101 cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt tactggggcg
    14161 ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg ttaaagcacc
    14221 ctggtctgca agtggtgccg gtggttgaac aaccggaagc aggctggcgt gggcgtactg
    14281 gtaccgtgtt aacggcggta ttgcaggatc atggtacgct ggcagagcat gatatctata
    14341 ttgccggacg ttttgagatg gcgaaaattg cccgtgacct gttttgcagt gagcgtaatg
    14401 cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg
    14461 cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcattt agatctatgt
    14521 cgcatccggc aagtggttaa acccgctcaa acaccgtcgc aataccctga cccagaccga
    14581 tacacatcgt cgccagacca aactgaacgt ctttgcgttc catcagattc agcagcgtgg
    14641 tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgca ccaccattga
    14701 ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatgcac ggcaggatct
    14761 gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc gctggcagaa agccccgctt
    14821 ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc cataatcgat gggtcacaac
    14881 caacgaccgc catcgaacgc acacgagcgc gcggcttaag acctaattca tgggcgcggc
    14941 tttcactcat caccagcatg gcagctgcgc catcggaaag tgcagaagat gtgcccgccg
    15001 ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt ggcgagggct tccacggtgg
    15061 tttccgggcg aatcacttcg tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac
    15121 caccagttgg gatgatttca tttttaaatg cggccgactg cgtggcggcc caggcgcggg
    15181 cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct gataccgtgt atacgcgcca
    15241 gcatttctgc cgttaagccc atcatgcccg ccgctttggc tacattgcgg ctcaggccgg
    15301 ggtgaaaatc gacgccgtga ctcatcggca catggcccat atgctccacg ccgccaacca
    15361 gacatgcctg cgcatcgcca gtcatgatca ttcgtgctgc gtcatgcagt gcctgcatgg
    15421 atgaaccaca caagcgattg acggtaaccg ccgggacaga gtgcggcact tctgccagca
    15481 gcgccgcgtt acgggcgatg ttaaaaccct gctccagcgt ctgttgcaca caaccccagt
    15541 aaatatcgtc gagggccgcc gcttccagcg ccgggttacg cgccagcagg ctacgcatta
    15601 aatgagcgga gagatcttcc gcacgcacgt tacgaaaagc accgcccttc gaacggccca
    15661 tcggggtgcg aattgcatcg acaatgacaa cctgttccat tgtgattcct taagccgttt
    15721 tcaggtcgcc aaccggacgg gctggctcaa ccggaggata gtacggttcg ttatgacgcg
    15781 ctttattacg cagaccttcc ggcacttcat acagcgggcc gaggtgctga tattgctgtg
    15841 ccatatcgag gtattttgcg ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga
    15901 acggagggaa gcccaggccg tagaccagcg ccatatccgc ttccgccgga gtggcgataa
    15961 tgccctcttc cagacagcgc accacttcgt tgaccatcgg gatcatcatg cgggcgataa
    16021 tctcttcttc gctgaaatcg cgcttcggtt ggctcacttc tgccagcagg tcttcaacgg
    16081 cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc tttataacgc cagaaaccga
    16141 ggccgttctt ctgaccaaag cggttggcat caaacagcgc gtcgatggca tcgcggtaat
    16201 ctttctgcat ccgctgcggg aagcctgctg ccatgacagc ctgagcgtga tgcgcggtat
    16261 caatgcccac aacgtccagc agatatgccg ggcccatcgg ccagccaaac tgtttttcca
    16321 tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga
    16381 aatacgggaa cagcacgcgg ttaacaaaga agccggggca gtcgttaacc acaatcggcg
    16441 tcttgcccat cttgctcgcc caggcgacaa ctttcgcgat ggtttcgtcg gagcttttct
    16501 cgccgcgaat aatttctacc aacggcattc ggtggaccgg gttaaagaag tgcatcccgc
    16561 agaagttttc cgggcgttcc agcgcgttgg ccagttcgct gataggaatg gttgaagtgt
    16621 tagacgccag cacggtatcc tggcgtactt tttgttcggt ttctgccagt acggcttttt
    16681 tcactttcgg gttttcaaca accgcttcta ccacaatatc cacgcggtca aatccggcgt
    16741 agtcgagcgt tgggtggatc gtggagatca cgccagccag tttcagacca tcgatcttgc
    16801 cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt catgccgagg gttaacgact
    16861 tgtcgttgat atctttcatg acaaccggca cgcctttcca cgcggactgg taagcgatac
    16921 cgccacccat gatgcctgcg cccagcacag cagcctgttt cggggtttca acgtctttgg
    16981 tgagtttctt cgctttgcct tttacatatt gatcgttaag gaaaatgccg accagtgcgc
    17041 gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt ttccagattt aaggcttctt
    17101 cacgaccaaa acgggccgca gcttcaatgg tttttactgc ggtgatgggg gccggataat
    17161 gtttccccgc cgtttgcgcg accattcctt tagcgatggt gaagctcatg gtggcttcaa
    17221 tcttgctcag ttttagcggt tccagcttcg gctgacgttt cgctttccag tcgaggtcgc
    17281 cgttaatggc ctggcgtaaa atcgccatcg ccccttcaat cagtttttct gctttgacta
    17341 cgccatccac cagaccgatt ttcagcgcct gatccgcgcc gacatcttta ccggcggcag
    17401 tgatttccag tgcactgtca gcgcccagca tacgtggcat acgtacagaa ccgccaaagc
    17461 ccggcatgat gcccagtttg gtttccggca gaccgatgcg cagatccggc gtcgccagac
    17521 gataatcggt cgccagcacg cattcgcagc caccacccag cgcatagccg ttgacggcag
    17581 caatggtcgg caccggcaga tcttccaggc gattaaacac gctattggca aagtgcagcc
    17641 actgacttaa ctgttcttca ggaacgagga acagggacaa aaattcggtg atatcagcac
    17701 cgacgataaa ggctgctttg ttcgaacgca gcagcagccc ttttagatct gattgctgtt
    17761 ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg
    17821 aacctggggc atcaaatacc agttcggcaa tgccatcttc cagccagtca aggtacaggg
    17881 tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa ggtgatctgg tcgtaccaga
    17941 tgcatcggag tgtgtgtttt gtgttaaaaa tatgcaaata aaagattaaa gaaatgccga
    18001 tctgatcacg ctcggcagaa atcacgctct ggatgaacga tgtgctaaga tgcggagact
    18061 taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct ataaaaatca
    18121 tatagctacc ttacaagaac ggactcgcga tgcgctggcg cgcttcaagc tggatgcatt
    18181 acttattcac tccggcgagc tgttcaacgt ttttctcgac gatcatccct atccgtttaa
    18241 agtgaacccg caattcaaag cgtgggtgcc ggtaactcag gtgccaaact gctggttgct
    18301 ggtggatggc gtgaacaagc cgaaactgtg gttctatctg ccggttgatt actggcacaa
    18361 cgtcgaaccg ctgccgacct ccttctggac tgaagatgtg gaagtgatca cgctgccgaa
    18421 agccgatggc attggtagcc tgctgcctgc tgcgcgcggc aatatcggtt atatcggtcc
    18481 ggtgccggaa cgtgcgctgc aactgggtat tgaggccagc aacatcaacc cgaaaggggt
    18541 gatcgactac ctgcattatt accgctcctt caaaaccgag tacgaactgg cctgtatgcg
    18601 tgaagcgcag aaaatggcgg tcaatggtca tcgtgcggca gaagaagcgt tccgttctgg
    18661 catgagcgag ttcgatatca atattgccta tctgaccgcg accggtcatc gtgataccga
    18721 cgtaccttac agcaacattg tggcgcttaa cgaacacgct gcggtgttgc attacaccaa
    18781 actggaccac caggcaccgg aagagatgcg cagcttcctg ctggatgccg gggccgaata
    18841 taacggctat gcggctgacc tgacccgtac ctggtcggca aaaagcgaca acgactacgc
    18901 acagctggtg aaagacgtaa atgatgaaca actggggctg atcgccacca tgaaagcagg
    18961 cgtcagctat gtggattacc acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa
    19021 acatcaaatc atcaccgata tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc
    19081 gtttatgccg cacggtattg gccatccgct gggcctgcag gtgcatgacg tcgccggttt
    19141 tatgcaggat gatagcggta cacacctcgc ggcaccggca aaatatccgt acctgcgctg
    19201 cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct acttcattga
    19261 atcgcttctg gcaccgtggc gtgaagggca gttcagcaag cacttcaact ggcagaaaat
    19321 tgaagcactg aaaccgttcg gcggcattcg tatcgaagac aacgtggtga tccacgaaaa
    19381 caacgtggaa aacatgaccc gggatctgaa actggcgtga tggaaagctg gttaattcct
    19441 gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc gcttcataac gatgttggcg
    19501 cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc
    19561 gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc tcaacagctg
    19621 ggtttctctg acgacgggga gccggcggga acggcaggta aaccgatgct cgcccagcta
    19681 atgggcagcg gcgtcgggga aattaccgct gtggtagtgc gctactacgg cggcatattg
    19741 cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag
    19801 ctaacgaccc aacgcaagac gccattaacc gaatatactt tgcaatgtga atatagtcag
    19861 ttaaccggca ttgaagcgtt gctggggcag tgtgacggca aaattatcaa cagtgattat
    19921 caggcattcg ttctgctgcg ggtggcgctt ccggcggcga aagtggctga attttccgca
    19981 aagctggcgg attttagccg tggttcattg caattgttag cgattgaaga ataatcccca
    20041 cttcgttttg cagaactaag gaagcggcag agatgcattt tcgcgccatt acccgaatcg
    20101 ttggactact ggtcatctta ttttcaggga ccatgattat ccctgggctg gtagcactca
    20161 tctaccggga tggagcgggc cgcgctttta cccagacctt ttttgtcgcc ctcgccattg
    20221 gctctatgct gtggtggccg aaccgcaaag agaagggcga actgaaatcc cgtgaggggt
    20281 ttctgatagt ggtgctgttt tggaccgtgc tgggtagtgt cggtgcgctc ccttttatct
    20341 tctcggaaag cccgaacctc acgattaccg atgcgttttt tgaatctttc tctggcctga
    20401 ccaccaccgg ggccactacg ctggtggggc tggattcgct ccctcatgcc atcctctttt
    20461 atcgccagat gctgcaatgg tttggcggga tggggatcat cgtgttagcg gttgcgatac
    20521 tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc ggcccgctga
    20581 aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa aactctgtgg ttgatttatg
    20641 tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc cggaatggat gcctttgatg
    20701 ccatcggcca tagctttgcg actatcgcta ttggcggctt ctcgacacat gatgccagta
    20761 tcggttattt cgacagtccg gctattaaca ctatcattgc tatcttcctg ctgatctccg
    20821 gctgtaacta cggtctgcac ttttcactgt taagtgggcg tagtctgaag gtttattggc
    20881 gcgatccgga atttcgcatg tttatcggcg tacagtttac gctggtggtt atttgtacac
    20941 tcgtactgtg gtttcataat gtctacagtt cggcgctgat gacaattaac caggcgtttt
    21001 tccaggtggt atcgatggcg acaaccgccg ggtttacgac tgacagcatt gcccgctggc
    21061 cgctcttttt gccggtactg cttttatgtt cagcttttat cggcggttgc gccgggtcaa
    21121 cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt taagcagggg aaccgtgagc
    21181 tgaaacgact ggtgcatccg aacgccgtct atagcattaa gctggggaat cgcgcactgc
    21241 cggaacgtat cctcgaagcc gtttggggat ttttctccgc ctatgcattg gtgtttattg
    21301 tcagtatgct ggcgattatc gccacgggcg tggatgattt ttctgccttt gcctcagttg
    21361 ttgcgacatt gaataacctg gggccagggc ttggcgtggt tgctgataac tttaccagta
    21421 tgaacccggt ggctaaatgg atcctgattg ccaacatgct gtttggtcgt ctcgaggtct
    21481 ttacattgct ggtgctcttt accccgactt tctggcgtga atgatggagt aatacgtgaa
    21541 aacattaatt cttttctcaa caagggacgg acaaacgcgc gagattgcct cctacctggc
    21601 ttcggaactg aaagaactgg ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga
    21661 accacaatgg gaaaactatg accgtgtggt cattggtgct tctattcgct atggtcacta
    21721 ccactctgcg ttccaggaat ttgtcaaaaa acatgcgacg cgactgaatt cgatgccgag
    21781 cgccttttat tccgtaaacc tggtggcgcg caaaccggag aagcgtactc cacagaccaa
    21841 cagctacgcg cggaagtttc tgatgaactc gcaatggcgt cccgatcgct gcgcggtcat
    21901 tgccggggcg ctgcgttacc cacgttatcg ctggtacgac cgttttatgc tcaagctgat
    21961 tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa gttgtctata ccgattggga
    22021 gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc gacaaaccga cgctgaaata
    22081 agcataaaga ataaaaaatg cgcggtcaga aaa
//
LOCUS       DADLTI010000058        21796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLTI010000058 DADLTI010000000
VERSION     DADLTI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.058"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..157
                     /locus_tag="JN755_001986"
     CDS             <1..157
                     /locus_tag="JN755_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7380791.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JN755_001987"
     CDS             227..1033
                     /locus_tag="JN755_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7380792.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JN755_001988"
     CDS             1043..1450
                     /locus_tag="JN755_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7380793.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JN755_001989"
     CDS             1450..1944
                     /locus_tag="JN755_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY7380794.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JN755_001990"
     CDS             2010..2807
                     /locus_tag="JN755_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7380795.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JN755_001991"
     CDS             2818..3606
                     /locus_tag="JN755_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7380796.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JN755_001992"
     CDS             3658..4887
                     /locus_tag="JN755_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7380797.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JN755_001993"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JN755_001993"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7380798.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JN755_001994"
     CDS             complement(6049..7680)
                     /locus_tag="JN755_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7380799.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JN755_001995"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JN755_001995"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7380800.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLGEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JN755_001996"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JN755_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7380801.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JN755_001997"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JN755_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JN755_001998"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JN755_001998"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7380802.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JN755_001999"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JN755_001999"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7380803.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JN755_002000"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JN755_002000"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7380804.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JN755_002001"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JN755_002001"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7380805.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21727
                     /locus_tag="JN755_002002"
                     /pseudo
     CDS             21287..21727
                     /locus_tag="JN755_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5369 a         5580 c         5520 g         5327 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggagctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgcc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcatc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcatggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccgaagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc ccccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaattgaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ctttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac tttctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagc cggttgggtc
    21481 tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgtgcggctg
    21541 cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac tgacaaccaa
    21601 cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
    21661 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt
    21721 gggggataag ccgcccctgc cgtgcctgcc caagctggag aagacgcgcc agcgtcgcac
    21781 ccggcatttt tttcac
//
LOCUS       DADLTI010000059        21518 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLTI010000059 DADLTI010000000
VERSION     DADLTI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.059"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JN755_002003"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JN755_002003"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7380806.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JN755_002004"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JN755_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7380807.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JN755_002005"
     CDS             complement(2649..3200)
                     /locus_tag="JN755_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7380808.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JN755_002006"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JN755_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7380809.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JN755_002007"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JN755_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7380810.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JN755_002008"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JN755_002008"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7380811.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JN755_002009"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JN755_002009"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7380812.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JN755_002010"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JN755_002010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7380813.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JN755_002011"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JN755_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JN755_002012"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JN755_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7380814.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JN755_002013"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JN755_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7380815.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JN755_002014"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JN755_002014"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7380816.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JN755_002015"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JN755_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7380817.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JN755_002016"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JN755_002016"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7380818.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JN755_002017"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JN755_002017"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7380819.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JN755_002018"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JN755_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7380820.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JN755_002019"
     CDS             complement(18174..18569)
                     /locus_tag="JN755_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7380821.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JN755_002020"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JN755_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7380822.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JN755_002021"
     CDS             19485..20078
                     /locus_tag="JN755_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7380823.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JN755_002022"
     CDS             20223..20675
                     /locus_tag="JN755_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7380824.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21518
                     /locus_tag="JN755_002023"
     CDS             20798..>21518
                     /locus_tag="JN755_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7380825.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5203 a         5329 c         5662 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttattgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tgccgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaata
//
LOCUS       DADLTI010000060        21498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLTI010000060 DADLTI010000000
VERSION     DADLTI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.060"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..2265
                     /locus_tag="JN755_002024"
                     /pseudo
     CDS             1..2265
                     /locus_tag="JN755_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2266..3915
                     /locus_tag="JN755_002025"
     CDS             2266..3915
                     /locus_tag="JN755_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY7380826.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQCQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3920..4696
                     /locus_tag="JN755_002026"
     CDS             3920..4696
                     /locus_tag="JN755_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7380827.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4971..7820
                     /gene="rcsC"
                     /locus_tag="JN755_002027"
     CDS             4971..7820
                     /gene="rcsC"
                     /locus_tag="JN755_002027"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7380828.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN755_002028"
     CDS             complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN755_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7380829.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN755_002029"
     CDS             complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN755_002029"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7380830.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            12097..13218
                     /gene="ompC"
                     /locus_tag="JN755_002030"
     CDS             12097..13218
                     /gene="ompC"
                     /locus_tag="JN755_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7380831.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13330..14385
                     /gene="apbE"
                     /locus_tag="JN755_002031"
     CDS             13330..14385
                     /gene="apbE"
                     /locus_tag="JN755_002031"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7380832.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14459..15523
                     /gene="ada"
                     /locus_tag="JN755_002032"
     CDS             14459..15523
                     /gene="ada"
                     /locus_tag="JN755_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7380833.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15523..16117
                     /gene="alkB"
                     /locus_tag="JN755_002033"
                     /pseudo
     CDS             15523..16117
                     /gene="alkB"
                     /locus_tag="JN755_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            16193..17836
                     /gene="yojI"
                     /locus_tag="JN755_002034"
     CDS             16193..17836
                     /gene="yojI"
                     /locus_tag="JN755_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7380834.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18054..18413
                     /locus_tag="JN755_002035"
                     /pseudo
     CDS             18054..18413
                     /locus_tag="JN755_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18647..19678
                     /locus_tag="JN755_002036"
     CDS             18647..19678
                     /locus_tag="JN755_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY7380835.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENLDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(19828..20316)
                     /gene="eco"
                     /locus_tag="JN755_002037"
     CDS             complement(19828..20316)
                     /gene="eco"
                     /locus_tag="JN755_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7380836.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            20724..21218
                     /gene="napF"
                     /locus_tag="JN755_002038"
     CDS             20724..21218
                     /gene="napF"
                     /locus_tag="JN755_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7380837.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21208..21471
                     /gene="napD"
                     /locus_tag="JN755_002039"
     CDS             21208..21471
                     /gene="napD"
                     /locus_tag="JN755_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7380838.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         5207 a         5245 c         5763 g         5283 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcctgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ttggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaaatg tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt
     1801 ccggtgaccg ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt
     1861 actctgcagc cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     1921 acgcttacct gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     1981 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     2041 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     2101 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     2161 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     2221 agtgtcgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     2281 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     2341 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     2401 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     2461 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     2521 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     2581 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     2641 gggcaatact ggcaacagtg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     2701 ccagaaacct cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa
     2761 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtctca
     2821 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     2881 gaaatccgtc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     2941 ggcagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     3001 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     3061 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccag cgtacttaat
     3121 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     3181 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     3241 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cacctgaggc agcgaaagcc
     3301 atgactgtgg cgatacgtac ttttttacag caaaacgcca atcgggaagg tgattgcctg
     3361 accatccccg gcagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     3421 cgaacgatga ccgcctggac gcaggagctc atctatgcag gcgatccggt tcattatcat
     3481 ggcagtcgcg ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag
     3541 ggggagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     3601 ggagctccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     3661 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     3721 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     3781 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacttg
     3841 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     3901 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     3961 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     4021 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     4081 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc agcggcaaaa taaagaatta
     4141 tgctgcggcg ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca
     4201 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     4261 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     4321 gggtacagga actattcgtg cacgtttacg gctggttctc tcgtgggata tggacaatac
     4381 cgatctcgat ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     4441 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     4501 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     4561 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     4621 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     4681 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     4741 ctgctgggcg atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca
     4801 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg
     4861 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     4921 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     4981 ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag
     5041 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     5101 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     5161 aacgcacctc tgatgtgatg aaagagctta agtacatcgc cgaaaatcgc ttatcggcag
     5221 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     5281 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     5341 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     5401 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     5461 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     5521 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     5581 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     5641 gtgtcgagca gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg
     5701 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     5761 ttaagggcga tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc
     5821 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     5881 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     5941 tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc
     6001 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     6061 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     6121 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     6181 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     6241 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     6301 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     6361 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     6421 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     6481 tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     6541 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     6601 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     6661 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     6721 tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg
     6781 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     6841 ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggttg
     6901 gaacgggcgt acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     6961 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     7021 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     7081 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     7141 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg
     7201 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggcaga gcggtagtga
     7261 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     7321 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     7381 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     7441 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
     7501 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
     7561 ttagtaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
     7621 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
     7681 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctct
     7741 cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca
     7801 ggaaatcgcg ggattcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
     7861 aatgccagat aagacactga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat
     7921 aatgggaatc gtaggccgga taaggcgttt acgccgcatc cggcaatcgg tgcaaatgcc
     7981 agataagaca ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact
     8041 taaggtcaca gaagagagat aattcagcag ggcgatatcg ttctcgacgc ccagcttcat
     8101 catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat
     8161 ctcggtcact aggaggcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag
     8221 acgcttgtca ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg
     8281 ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc
     8341 ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt
     8401 gttgttcata gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat
     8461 taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg
     8521 cgcatccagt ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac
     8581 attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc
     8641 atcggcaata attacgttca tattgttcat gtaataggct accttgctac agcaagctct
     8701 tgacataact gtcaatgtcg ctgatgtatt tttctattct tggaacatcc ttctcacgaa
     8761 tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata
     8821 cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag
     8881 tatacagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg
     8941 catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca
     9001 gttgcacttc aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga
     9061 cgcacaattg accaggccca atttctcgta cgccagactc atcatcgctt aaaagcaagc
     9121 cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta
     9181 atctttcatc gggtgtgata caggttgcac cccaattttc taacaggcga gtgacaatat
     9241 tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt
     9301 cttcctcttc aacttccggg tcagcggcga gcattttgat atgcacagag tagcgtgtac
     9361 caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc
     9421 tcagccagaa tgccagcggg tccgcctttc cataacgatc gttttgggtc tggttgataa
     9481 acgggaagtg caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc
     9541 ggaatgtcag gcggtcttca gaggactcat cctgatcaac ctcaagggtg atttttccca
     9601 attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc
     9661 gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt
     9721 tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga
     9781 acagcaccgt ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttctt
     9841 cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga
     9901 gtttagccgc actctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt
     9961 tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt
    10021 tcttgtttac cagcacttcg cgatcctgat cgcgaataat gagaatttgt gtgcgcggcg
    10081 cgacctggct gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa
    10141 tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcagatgc ggcagcaaat
    10201 gatcggcaat tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc
    10261 cgagcggcaa cagtgagact atctcttcat taattgcccg taaaatgcgc aattcgttat
    10321 tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat
    10381 agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt
    10441 gcaacgtatc cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag
    10501 agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg
    10561 tcccttcttt ctcattatca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac
    10621 tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg
    10681 ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc
    10741 aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca
    10801 acatttctgc acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg
    10861 tgagatcttt aaggggtagg gttgagatca gcaccagact gttatcctga ccattcaggt
    10921 aatacatcga ccacggtaca ttttctgcac cccacaatgt atccagataa gtggacatcc
    10981 gctgagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc
    11041 ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc
    11101 cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgcctggtc acgtaacgcc
    11161 aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt
    11221 tcagccaggc gttaacggca ctttgtacca ttacccccat cgtcaccagt aacacaatga
    11281 tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg
    11341 ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc
    11401 agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc
    11461 ccgtgatatt tatcatcctg tatgggggga aatagatgta caagcgccat tttgctgtac
    11521 aaataaccta caaaaagccc aacgaaatta attgtaaata aacaaagatt gctggaaatt
    11581 atgctgatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca
    11641 cagaataatg aaaagtgtgt aaagaagggt aaaaaaaccg aatgcgaggc atccggttga
    11701 aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt aatgatgata
    11761 gcgggagtta ttctagttgc gagtgaaggt tttgttttga cattcagtgc tgtcaaatac
    11821 ttaagaataa gttattgatt ttaaccttga attattattg cttgatgtta ggtgcttatt
    11881 tcgccattcc gcaataatct taaaaagttc ccttgtattt acattttgaa acatctgtag
    11941 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
    12001 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
    12061 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
    12121 ctggtcccag ctctgctggt agcaggagca gcaaacgctg ctgaagttta caacaaagac
    12181 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacgacaag
    12241 tctgtagatg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
    12301 gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac
    12361 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct
    12421 ttcgactacg gtcgtaacta cggcgtagtt tacgacgtaa cttcctggac cgacgtactg
    12481 ccagaattcg gtggtgacac ttacggttct gacaacttca tgcagcagcg tggtaacggc
    12541 ttcgcaacct accgtagcac cgacttcttt ggtctggttg acggtctgaa cttcgctgtt
    12601 cagtatcagg gcaaaaacgg tagcccggaa ggtgaaggca tgaccaacaa cggtcgtgaa
    12661 gctctgcgtc agaacggtga cggcgttggc ggttctatta cttatgatta cgaaggcttc
    12721 ggtatcggtg ctgcagtttc cagctccaaa cgtactgatg accagaactt cggtctgaac
    12781 cgttatgatg agcgttacat cggtaatggc gaccgcgctg aaacctacac cggtggtctg
    12841 aaatacgacg cgaacaacat ctacctagct gctcagtaca cccagaccta caacgcaact
    12901 cgcgtaggca acttgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac
    12961 cagttcgact tcggtctgcg tccgtcccta gcttacctgc agtctaaagg taaaaacctg
    13021 ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt tgatgttggt
    13081 gcgacctact acttcaacaa aaacatgtcc acctacgttg actacaaaat caacctgctg
    13141 gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt
    13201 ctggtttacc agttctaatc tcgatggata tcgaacaaag ggcctgcggg cccttttttc
    13261 attgttttca gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt cgcatgagga
    13321 taatcacgta tggaaataag ctttacccgc gtggcactgc tggttgccgc gctcttcttt
    13381 gttggttgcg atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt
    13441 gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc cgggcattga tgccaaacgc
    13501 agtgccgaac ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg
    13561 acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt gtcgccgtgg
    13621 ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tgcgcattgg ggcaaggacc
    13681 gatggtgcga tggatattac cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa
    13741 caacagccgg ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc
    13801 ttacagcacc tgacggtcat caatcagtcg catcagcaat atctgcaaaa agacctgccg
    13861 gatttatatg tcgatctctc caccgtcggt gaaggttatg cggcggatca cctggcacgc
    13921 ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc
    13981 cgtggtatga acggtgaagg ccagccgtgg cgggtagcga ttcaaaaacc aaccgataaa
    14041 gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg ggattagcac ttctggcagc
    14101 taccgtaact attacgaact ggacggcaaa cgtctttccc atgttatcga tccgcaaacc
    14161 gggcgtccca tcgaacacaa tctggtatcc gtgacggtga ttgctccgac ggcgctggaa
    14221 gccgatgcct gggatactgg cttgatggta ctcgggccgg agaaagccaa agaggttgtt
    14281 cgccgggaag ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg
    14341 atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgttg
    14401 gtttttgcgt gatggtgacc gggcagacta aaggctatcc ttaaccaggg agctgattat
    14461 gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc
    14521 ggatgccgac ggcgaattcg ttttcgccgt gcgcaccacg ggtatctttt gccgtccgtc
    14581 ttgccgcgcc agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg ccagcgaggc
    14641 actcgccgcc ggatttcgcc cctgcaaacg ttgtcagcca gacaaagcca atccccggca
    14701 acatcggttg gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac
    14761 gctggaagcc ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa
    14821 agctactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg
    14881 cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga
    14941 cagcagcagt tactaccgca aagccgatga aacgctgggc atgacggcta aacaattccg
    15001 tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg
    15061 cctggtggca gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac
    15121 attaatcagc gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgac
    15181 gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac
    15241 gttaccgctg gatattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac
    15301 gataccttgt ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa
    15361 agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg taataccctg
    15421 tcatcgagtg gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa
    15481 agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc
    15541 gatgctgaac cgtggcaaga gccactggcg gctggtgcgg taattttacg gcgttttgct
    15601 tttaatgctg cggagcaact aatccgcgat attaatgacg ttgccagcca gtcgccgttt
    15661 cgccagatgg tcgctccagg cggatatacc atgtcggtgg cgatgaccaa ctgtgggcat
    15721 ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat
    15781 aaaccgtggc ccgccatgcc acaaagcttt catgatttat gtcaacgtgc ggctacggcg
    15841 gcgggctatc cagatttcca gccagatgct tgtcttatca accgctacgc tcctagcgcg
    15901 aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt
    15961 tctctgggct tacccgcgat ttttcaattt ggtggcctgg ggcggtgaat cgcggctgtt
    16021 ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccaccg actgccgcta
    16081 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
    16141 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
    16201 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct
    16261 cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac
    16321 ggcggatacc agtctgctgg tgttaccgga gtttctggga ttattgctgc tgttgatggc
    16381 agtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
    16441 gcgtagtgaa tttatcaagc ggattctgga tacccacgtc gagcgcattg aacaactcgg
    16501 tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
    16561 gcgtctgccg gaactggggc aggggatcat tctcactatc ggttcatcgg cgtatctgtg
    16621 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcgatca ccatctgggg
    16681 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctacgtg aaaccgaaga
    16741 caagctgtac acggattttc aaactgtact tgaagggcgc aaagagctga ctctgaaccg
    16801 ggaacgcgcc gagtatgtgt ttaacaacct ctacatcccc gatgcgcaag agtatcgcca
    16861 tcatattatt cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat
    16921 gctgggcgct atcggcctgg tgttctggat ggcgaacagt ctcggttggg ctgataccaa
    16981 cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt
    17041 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
    17101 gctcgcgcct ttcaaagctg agtttccgcg cccgcaggcg tttcccaact ggcaaacgct
    17161 ggagctgcgt aacgtgacgt ttgcttatca ggataacgcg ttttccgttg gtccgattaa
    17221 tctcaccatc aaacgtggcg agctgctgtt tctgattggc ggcaacggta gcggaaaatc
    17281 gacgctggcg atgttgttga cgggcttgta tcagccacaa agcggcgaaa tcttgctgga
    17341 tggcaaacct gtcagcggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt
    17401 taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg ctaacccgca
    17461 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
    17521 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtgccgc tgttgctggc
    17581 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
    17641 cttccgtcgt gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat
    17701 tttcgctatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg
    17761 caatgggcaa cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt
    17821 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgtc aggtcaatat
    17881 gctaaaaatc cctttatttc taataaccct ataattaatc aacgaaatta taatgtttct
    17941 aaaattagaa tataatttat aaacattatt taaatgttgt tacttaagtg ttaccgttga
    18001 tgccgcgcaa atctcacctg caataaagcg actaaaagta aggcattaac aagatgaaaa
    18061 aagtgactgc catgctcttc tcgatggccg tggggcttaa tgccgtttcg atggcggcaa
    18121 aagcgaaagc gtccgaggag caggaaactg atgtactgtt gattggcggc ggcattatga
    18181 gcgccacgtt ggggacctat ttacgcgagc tggagcctga atggtcgatg accatggtgg
    18241 agcgcctgga ggggggcgcg caggagagtt cgaacggctg gaacaacgcc ggaaccgggc
    18301 attctgcact gatggaactg aactacaccc cgcaaaacgc cgatggcagc atcagtattg
    18361 aaaaagcagt cgccattaac gaagcatttc agatttcccg ccagttctgg gcgcaccagg
    18421 tcgagcgcgg cgtgctgcgt actccgcgtt catttatcaa taccgttccg catatgagct
    18481 ttgtctgggg cgaggataac gttaatttcc tgcgcgcccg ttacgccgcg ttgcaacaaa
    18541 gctcgctgtt tcgcggtatg cgttactctg aagatcacgc gcagatcaaa gagtgggcac
    18601 cgttagtgat ggaagggcgc gatccgcaac agaaattggc actgatgtga actatggtga
    18661 gatcacccgc cagttaattg cttccttaca gaagaaatct aacttctcgc tgcaactcag
    18721 cagcgaagtc cgcgccctaa agcgtaatga cgataacacc tggaccgtta ccgttgccga
    18781 tctgaaaaac ggcactgcac agaacatccg caccaaattt gtctttatcg gcgcgggcgg
    18841 tgcggcgctg aagctgttac aggaatcggg gattccggaa gcgaaagact acgccggttt
    18901 cccggtgggc ggacagttcc ttgtttcgga aaatctagac gtggttaatc gccatctggc
    18961 gaaagtttac ggtaaagcat ccgttggcgc accaccgatg tcggttccgc atatcgatac
    19021 ccgtgttctg gacggtaaac gcgtagtgct gtttgggcca tttgccacct tctcaaccaa
    19081 attcctcaaa aacggttcat tgtgggatct gatgagttcc accaccacct ctaacgtgat
    19141 gccgatgatg cacgtcgggc tggataattt cgatctggtg aaatatctgg tgagtcaggt
    19201 gatgttgagt gaagaggatc gttttgaagc gttgaaagag tactatccgc aagcgaaaaa
    19261 agaggactgg cgtttgtggc aagcggggca gcgtgtgcag attatcaagc gtgatgccga
    19321 gaaaggtggc gtactgcgtc tgggtaccga agtggtcagt gaccagcaag ggaccattgc
    19381 ggcgctgctg ggggcatcgc caggggcgtc aaccgccgcg ccgattatgt tgaatctgct
    19441 ggaaaaagta tttggcgatc gcgtttccag cccgcaatgg cagtctacgt tgaaagcgat
    19501 cgttccgtct tatggacgca agctgaacgg tgatgtagcg gcaacagaac gcgagttgca
    19561 gtacaccagc gaagtgctgg ggctgaaata cgacaaaccg caagcagcag atagtacgcc
    19621 gaaaccgcag ttgaaaccgc aacccgttca aaaagaagtg gcggatattg cgttgtaatg
    19681 atgtgccaca tccggcatgg tatgccggat gtggcgtata ctgataagac gcgccagcgt
    19741 cgcatcaggc aaccggctcg ggcgttagat gttggatgcg gcgtaaccgc cttatccaac
    19801 caacggggtg ccgtacttca caacagttta gcgaacttcc gcgttgtcaa ttttctcttc
    19861 cgccttccag acgcggtatt tcacatctac attgtctggc gtatacacca cgatcggcag
    19921 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
    19981 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
    20041 ataataatca tagccccagc cttccagcgt tttgctttcc agcttcccgc cgagacgatg
    20101 caaattgcaa tcgacttcca gcgtctgacc gattaacagt tctactttca gggtagattc
    20161 atcttcttgc ggggttaact gaatcacctg acgcttcatg cctgtttcag cttgtggata
    20221 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtagcgaa
    20281 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
    20341 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
    20401 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
    20461 atgagggagc aaacaagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
    20521 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
    20581 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
    20641 gcggctataa aaatggctta ttaattatgc ggtctatttg gttgctctca attttcagag
    20701 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
    20761 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
    20821 ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
    20881 aacgcggcac gggtggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
    20941 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
    21001 atttgcagtt caccatcggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
    21061 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
    21121 aggcgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
    21181 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
    21241 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
    21301 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
    21361 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
    21421 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
    21481 gtcgtagctt tatgaaag
//
LOCUS       DADLTI010000061        21418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLTI010000061 DADLTI010000000
VERSION     DADLTI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.061"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="JN755_002040"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="JN755_002040"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY7380839.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JN755_002041"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JN755_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7380840.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JN755_002042"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JN755_002042"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7380841.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JN755_002043"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JN755_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7380842.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JN755_002044"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JN755_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7380843.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JN755_002045"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JN755_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7380844.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JN755_002046"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JN755_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7380845.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JN755_002047"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JN755_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7380846.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JN755_002048"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JN755_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7380847.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JN755_002049"
     CDS             complement(8459..8785)
                     /locus_tag="JN755_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380848.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JN755_002050"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JN755_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY7380849.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JN755_002051"
     CDS             complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JN755_002051"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7380850.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JN755_002052"
     CDS             complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JN755_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7380851.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14953..15753
                     /gene="nagB"
                     /locus_tag="JN755_002053"
     CDS             14953..15753
                     /gene="nagB"
                     /locus_tag="JN755_002053"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7380852.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15813..16961
                     /gene="nagA"
                     /locus_tag="JN755_002054"
     CDS             15813..16961
                     /gene="nagA"
                     /locus_tag="JN755_002054"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7380853.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16970..18190
                     /gene="nagC"
                     /locus_tag="JN755_002055"
                     /gene_synonym="nagR"
     CDS             16970..18190
                     /gene="nagC"
                     /locus_tag="JN755_002055"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7380854.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18238..18980
                     /gene="nagD"
                     /locus_tag="JN755_002056"
                     /pseudo
     CDS             18238..18980
                     /gene="nagD"
                     /locus_tag="JN755_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19377..21041
                     /gene="asnB"
                     /locus_tag="JN755_002057"
     CDS             19377..21041
                     /gene="asnB"
                     /locus_tag="JN755_002057"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7380855.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         5262 a         5429 c         5405 g         5322 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgaaaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    10321 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    10381 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    10441 gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta
    10501 cattttattt aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg
    10561 aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg
    10621 ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca
    10681 atatattgca gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg
    10741 aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac
    10801 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
    10861 cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
    10921 gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac
    10981 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
    11041 gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
    11101 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
    11161 agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat
    11221 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
    11281 agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg
    11341 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
    11401 atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc
    11461 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
    11521 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
    11581 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
    11641 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
    11701 gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg
    11761 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat
    11821 gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
    11881 agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc
    11941 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
    12001 cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt
    12061 cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc
    12121 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
    12181 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata
    12241 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
    12301 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
    12361 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
    12421 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
    12481 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
    12541 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
    12601 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca
    12661 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    12721 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    12781 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    12841 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    12901 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    12961 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    13021 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    13081 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    13141 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    13201 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    13261 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    13321 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    13381 taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg
    13441 ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt
    13501 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    13561 ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc
    13621 tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc
    13681 cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca
    13741 ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    13801 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    13861 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga
    13921 agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc
    13981 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    14041 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    14101 aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg
    14161 gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg
    14221 gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac
    14281 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    14341 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    14401 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    14461 atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    14521 ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta
    14581 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    14641 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc
    14701 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    14761 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    14821 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc
    14881 tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta
    14941 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    15001 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta
    15061 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    15121 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    15181 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    15241 gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc
    15301 gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    15361 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    15421 actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    15481 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc
    15541 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt
    15601 gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc
    15661 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    15721 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct
    15781 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    15841 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    15901 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    15961 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    16021 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    16081 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    16141 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    16201 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    16261 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    16321 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    16381 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    16441 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    16501 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
    16561 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
    16621 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    16681 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    16741 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    16801 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg
    16861 gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    16921 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    16981 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    17041 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    17101 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    17161 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    17221 caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga
    17281 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac
    17341 gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct
    17401 gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    17461 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga
    17521 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    17581 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    17641 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
    17701 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    17761 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
    17821 gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc
    17881 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa
    17941 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat
    18001 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct
    18061 gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    18121 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    18181 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    18241 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    18301 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    18361 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    18421 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc
    18481 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    18541 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc
    18601 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc
    18661 gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    18721 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    18781 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    18841 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    18901 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    18961 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    19021 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    19081 aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc
    19141 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    19201 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    19261 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    19321 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    19381 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    19441 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    19501 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    19561 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    19621 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    19681 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    19741 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    19801 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    19861 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    19921 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    19981 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    20041 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    20101 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    20161 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    20221 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    20281 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    20341 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    20401 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    20461 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    20521 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    20581 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    20641 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    20701 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    20761 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    20821 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    20881 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    20941 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    21001 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    21061 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    21121 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    21181 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacgg caaaagaatg
    21241 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    21301 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    21361 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaag
//
LOCUS       DADLTI010000062        21239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLTI010000062 DADLTI010000000
VERSION     DADLTI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.062"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(32..1012)
                     /locus_tag="JN755_002058"
     CDS             complement(32..1012)
                     /locus_tag="JN755_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY7380856.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JN755_002059"
     CDS             complement(1020..1730)
                     /locus_tag="JN755_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7380857.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JN755_002060"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JN755_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7380858.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="JN755_002061"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="JN755_002061"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7380859.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="JN755_002062"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="JN755_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7380860.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="JN755_002063"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="JN755_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7380861.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /gene="yshB"
                     /locus_tag="JN755_002064"
     CDS             8306..8416
                     /gene="yshB"
                     /locus_tag="JN755_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7380862.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JN755_002065"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JN755_002065"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7380863.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JN755_002066"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JN755_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7380864.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="JN755_002067"
     CDS             11298..11930
                     /locus_tag="JN755_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7380865.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JN755_002068"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JN755_002068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7380866.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15100..15342
                     /locus_tag="JN755_002069"
     CDS             15100..15342
                     /locus_tag="JN755_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7380867.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15462..16394
                     /locus_tag="JN755_002070"
     CDS             15462..16394
                     /locus_tag="JN755_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7380868.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="JN755_002071"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="JN755_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JN755_002072"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JN755_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7380869.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JN755_002073"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JN755_002073"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7380870.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="JN755_002074"
     CDS             complement(19726..19995)
                     /locus_tag="JN755_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7380871.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="JN755_002075"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="JN755_002075"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7380872.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="JN755_002076"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="JN755_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7380873.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5119 g         5596 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct tttcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DADLTI010000063        21105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLTI010000063 DADLTI010000000
VERSION     DADLTI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.063"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..166
                     /locus_tag="JN755_002077"
     CDS             <1..166
                     /locus_tag="JN755_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein EutK"
                     /protein_id="HAY7380874.1"
                     /translation="EPDAPVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARN
                     ALEQLFSAGT"
     gene            251..1303
                     /gene="eutR"
                     /locus_tag="JN755_002078"
     CDS             251..1303
                     /gene="eutR"
                     /locus_tag="JN755_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7380875.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN755_002079"
     CDS             complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN755_002079"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7380876.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN755_002080"
     CDS             complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN755_002080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7380877.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            3295..3720
                     /locus_tag="JN755_002081"
     CDS             3295..3720
                     /locus_tag="JN755_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7380878.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3707..4156
                     /locus_tag="JN755_002082"
     CDS             3707..4156
                     /locus_tag="JN755_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7380879.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            4217..4792
                     /locus_tag="JN755_002083"
     CDS             4217..4792
                     /locus_tag="JN755_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7380880.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            4888..5787
                     /gene="yfeX"
                     /locus_tag="JN755_002084"
     CDS             4888..5787
                     /gene="yfeX"
                     /locus_tag="JN755_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7380881.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN755_002085"
     CDS             complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN755_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7380882.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN755_002086"
     CDS             complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN755_002086"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7380883.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            8254..9255
                     /gene="murR"
                     /locus_tag="JN755_002087"
     CDS             8254..9255
                     /gene="murR"
                     /locus_tag="JN755_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7380884.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            9442..10233
                     /gene="ucpA"
                     /locus_tag="JN755_002088"
     CDS             9442..10233
                     /gene="ucpA"
                     /locus_tag="JN755_002088"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7380885.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            10537..11553
                     /locus_tag="JN755_002089"
     CDS             10537..11553
                     /locus_tag="JN755_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7380886.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            11553..12386
                     /gene="cysT"
                     /locus_tag="JN755_002090"
     CDS             11553..12386
                     /gene="cysT"
                     /locus_tag="JN755_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7380887.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            12386..13261
                     /gene="cysW"
                     /locus_tag="JN755_002091"
     CDS             12386..13261
                     /gene="cysW"
                     /locus_tag="JN755_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7380888.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            13251..14348
                     /gene="cysA"
                     /locus_tag="JN755_002092"
     CDS             13251..14348
                     /gene="cysA"
                     /locus_tag="JN755_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7380889.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            14482..15393
                     /gene="cysM"
                     /locus_tag="JN755_002093"
     CDS             14482..15393
                     /gene="cysM"
                     /locus_tag="JN755_002093"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7380890.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15597..16320)
                     /locus_tag="JN755_002094"
                     /pseudo
     CDS             complement(15597..16320)
                     /locus_tag="JN755_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16353..16727)
                     /locus_tag="JN755_002095"
     CDS             complement(16353..16727)
                     /locus_tag="JN755_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7380891.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            16832..17683
                     /gene="pdxK"
                     /locus_tag="JN755_002096"
                     /gene_synonym="thiJ"
     CDS             16832..17683
                     /gene="pdxK"
                     /locus_tag="JN755_002096"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7380892.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN755_002097"
     CDS             complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN755_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7380893.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN755_002098"
     CDS             complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN755_002098"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7380894.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN755_002099"
     CDS             complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN755_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7380895.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(20688..>21105)
                     /locus_tag="JN755_002100"
     CDS             complement(20688..>21105)
                     /locus_tag="JN755_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY7380896.1"
                     /translation="SRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGEEIKPGPHKIQG
                     IGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQED
                     ESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
BASE COUNT         5002 a         5260 c         5680 g         5163 t
ORIGIN      
        1 ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg cgctgttaac
       61 atcagtatgt cagggaatga cggcaggaga agtggccgcc cactttggct ggccgctgga
      121 aaaagccaga aatgcgctcg aacagctctt ttctgccggg acgtaacgta aacgcagtag
      181 tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc gggtgtccta caacacccgg
      241 cattaacatc atgaaaaaga cccgtacagc caatttgcac catctttatc atgaaccctt
      301 acccgaaaac ctgaagctca cgccgaaggt cgaagtggat aatgttcatc aacgacagac
      361 aacggatgtc tatgaacatg ctttgacgat taccgcctgg cagcagattt acgatcagct
      421 gcatccgggc aagtttcatg gtgaatttac ggaaattcta ctcgatgata ttcaggtttt
      481 tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg gtctggccga actcgttctg
      541 gtttggcatt ccggcgacgc gcggtgagca gggatttatc ggttcgcaat gtctgggaag
      601 cgcagaaata gcaacgcgcc ccggtggaac cgagtttgaa ttaagtacgc cggacgatta
      661 tacgatcctg ggcgtagtgc tgtctgaaga tgtcatcacc tgtcaggcta actttttgca
      721 caatccggat cgggtattac atatgctgcg tagccagtcg gcgctggaag tgaaagagca
      781 gcataaagcc gcgctgtggg gctttgtcca acaggcgctg gcgacgtttt gcgagaaccc
      841 ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg gataatttgc taatggcgat
      901 gggggcgatg ctggaagaag cgcaaccaat ggtgacggcg gaaagcatca gtcatcagag
      961 ttaccgtcgg ttgctttccc gcgcccgtga atatgtgctg gaaaacatgt ccgaaccggt
     1021 gacggtgctg gatttgtgta atcaactgca tgtcagtcgc cgcacgctac aaaacgcgtt
     1081 tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa cgcattcgcc tgaacgccgt
     1141 acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg gtaaaagacg ccgccatgca
     1201 gtggggattc tggcatctgg ggcaatttgc cacggattac cagcagctgt ttgccgagaa
     1261 gccgtcgttg acgttgcatc agcggatgcg ggaatggggg tgagggagtt acacccaatc
     1321 cctgacctta ataaactcac ttaacgccgc ttctgggctg ccatcttttg gctgataatc
     1381 atactcccag cgtaccagtg gcggcattga catcaggatc gactcggtgc gcccgccagt
     1441 ttgcaggcca aacagcgtgc cgcgatccca gaccagattg aactcgacat aacgaccgcg
     1501 acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc atcgctttac gtcgctctac
     1561 aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc tgcataaagg caaaacagtg
     1621 gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg ccaatgccgc gctgttcgtt
     1681 gcgatgtttg aggtagaagt aatcgtcgca ccactttttg taacggggat aaacgtcttc
     1741 accaaatggc tggcacaggt cacgggcggt gcggtgccag tgaatggcgt cttcttcaaa
     1801 accatagaaa ggggttaaat cgaagccgcc gccaaaccac caaacgggct ctgcacccgg
     1861 tttttcggca ataaaaaaac gcacattcgc gtggctggtg ggaacatacg ggttatgcgg
     1921 atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc ccggcaagtt ccgggcgatg
     1981 agcggtggcg gaagcaggca tcgcctcacc gtggacgtga gaaaagttga cgcccgcctg
     2041 ttcgaaaaca ccaccattac gcaacacccg actgcgcccg ccgccgccag cttcgcgctg
     2101 ccaactatct tcgacaaatt ctgcgccatc gacggcggtc agctgctgac aaatcgtatc
     2161 ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg ggtttcataa cttatcgctt
     2221 tttcgaatgt gctttctggt tgtcgaacca gtggaaataa ctgatcacgc cttcagcaat
     2281 cgctgtggcg attttctgac gaaacgccgc cgtgcctaac agccgctctt cttccgggtt
     2341 ggtgataaac gaggtttcca ccagcaccga aggaaccgac ggcgatttca acaccacaaa
     2401 cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc ggcttaatct ttttcagaat
     2461 atgcgagcca agcgtcaggc tgtttttaat agtatcggtt tgcaccagat caaacagtac
     2521 ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg gcaacttcat cggcgcggtt
     2581 ttcgcgttca gacaggtatt tcgccattgc gctacttgcc ccacggttag agagggcaaa
     2641 taccgaagca ccggcagctt tcgggttggt aaagccatcg gcatgaattg acataaacag
     2701 atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa agtgggataa acgtatcgcc
     2761 agaacgcgtt aaacgcgcat caatcccatg attacgcaaa atggaacgga cgtttttagc
     2821 aatcgccagc accacatgtt tttctttcga accgttgcgt ccgatcgctc cggtatcaat
     2881 tccgccgtgg cctggatcga gaacaacgac acgtttgccg ccagattttt ttgctttcgg
     2941 cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg gcaatggctt gcgacattcc
     3001 tgacaacgtc agggcagcta aaccggcttt cagcacctgg cggcgcgaag tgagtgtttt
     3061 tagtggttta aaagtgctca tacggcctga gttgtaataa taaagttcca gatgttatat
     3121 cgtatcgcgt aatccgttac gacggtttgc tgttgagaat tgttttactt ttcatttcaa
     3181 tagttgacaa ctccccatta tgccactttt tcaaccgatt ttcgtcagat attggctaat
     3241 tcgaccgttc acgccataat cagcctttta acccgcaaaa aggtaacgaa taccatggag
     3301 atacgcgtat ttcgccagga agatttcgaa gaggtcatca ccctttggga gcgttgcgac
     3361 ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc gtaagatgaa ccatgacgtc
     3421 agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg gaacggtgat gggcggttat
     3481 gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc cagagtttcg tgggcgtgga
     3541 attgccaatg cgttgcttaa ccggctggag aaaaagctga ttgctcgcgg ctgcccgaaa
     3601 attcagatca acgtgccgga agataacgac atggtgctcg gaatgtatga acgcctggga
     3661 tatgaacacg ccgacgtgct gagtctgggt aagcgtttga ttgaagatga agagtactga
     3721 gttccatcct gtccattatg atgcgcacgg acgcctgcgt ttacccttgc tcttctggct
     3781 tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc attgccggtg cgtcgcgcga
     3841 gcagggtact gcgctgctaa atctgtttta tcccgatcac gataatttct ggctggggct
     3901 gattcccggt attccggcgg tgctggcgtt tttgttgagc ggtcggcggg cttcgtttcc
     3961 tcgcacctgg catgtgctct attttctgtt gctgttggcg caggtggttt tactttgctg
     4021 gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt atcggtctgg cgctggtagt
     4081 ggcggatatt gtggcgttaa tctggctgct gaccaatcga cgtttacgcg cttgttttaa
     4141 tgaagaaaaa gaataacggc actttttggt gaatttgcac tccaagcaac gttattgaat
     4201 aaccaaagga agtgacatga aatcgctgcg tttaatgtta tgcgctatgc cgttgatgct
     4261 gaccggctgt tccacgatgt cgtcagttaa ctggtctgcc gctaacccgt ggaactggtt
     4321 tggatcgtcc accaaagtga gcgagcaggg cgtgggtgaa ttaacggcgt ccacaccact
     4381 gcaagaacaa gccattgctg atgcgcttga tggcgattat cgcctgcgca gcggaatgaa
     4441 aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg aaaggcgaca acgtggcgat
     4501 ggtgattaac ggcgatcaag gcacgatcag tcgcattgac gtgctggata gcgatattcc
     4561 tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac ctttacagca aagcatttgg
     4621 caattgccaa aaagctgatg gtgatgataa tcgtgccgtc gaatgtaaag ccgaaggcag
     4681 tcaacatatt agctaccagt tcagcgggga atggcgtggt cctgaagggt taatgccttc
     4741 ggacgatacc ctgaaaaact ggaaagtcag taaaattatc tggcggcgtt aatttgcgtc
     4801 tgaacaaacc gccgtcgcga aaaaacacgt aaaatagcgc tcaacaatgc cacggattgc
     4861 gtggcattct tattttcagg aggaacaatg tctcaggttc agagtggtat tttgccagaa
     4921 cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag gggaagttga cgccctgcgt
     4981 gcggccagta aaacatttgc cgacaaactg gcaacttttg aagcgaaatt cccggacgcg
     5041 catcttggtg cggtggttgc ctttggtaac aacacctggc gcgctctgag cggcggcgtt
     5101 ggggcagaag agctgaaaga ttttccgggc tacggtaaag gccttgcgcc gacgacccag
     5161 ttcgatgtgt tgatccacat tctttctctg cgtcacgacg taaacttctc tgtcgctcag
     5221 gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag aagagatcca cggcttccgt
     5281 tgggtggaag agcgtgacct gagcggcttt gttgacggta cagaaaaccc ggcgggtgaa
     5341 gagacgcgcc gcgaagtggc ggttatcaaa gacggcgtgg atgccggcgg cagctatgtg
     5401 tttgtgcagc gttgggagca caacctgaag cagctcaacc ggatgagcgt tcacgatcag
     5461 gagatgatga tcgggcgcac caaagaggcc aacgaagaga ttgacggcga cgaacgtccg
     5521 gaaacctctc acctcacccg cgttgatctg aaagaagatg gcaaagggct gaagattgtt
     5581 cgccagagcc tgccgtacgg caccgccagt ggcactcacg gtctgtactt ctgcgcctac
     5641 tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca tgtttggcga taccgatggc
     5701 aaacgtgatg cgatgttgcg tttcaccaaa ccggtaaccg gcggctatta tttcgcaccg
     5761 tcgctggaca agttgatggt gctgtaagcg tttctgattc attgattaaa ggccaggcag
     5821 taacattact gactggcctt tttattactg ctttaacgcc gcatatactt gatcaactac
     5881 cttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa
     5941 gatagagcat tgtccgtttc ataatatcag tccagattga cgttacggca gccaaagagc
     6001 gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatt
     6061 gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ccaccagacc agacggccca
     6121 aaggcgctgt ttaagcccat cggcagaccc caccaggcta tcaatccgat aaacaaaccg
     6181 cccgccgcgc cgcctaaaca ggcggtaaca aacggcttca tgcggggcag ggtgacaccg
     6241 taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg tacctgactg
     6301 cgtagcgcac tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc
     6361 gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctggctgtc cattaacgcg
     6421 aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg
     6481 gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct
     6541 tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc
     6601 agggtgatta acgaggtcag cagcatgtcg agatcgtccg gcataaagcg gcgcaccatg
     6661 ccttcaatgc gggcgcaggc cctcgcggca atcagcacgc cgataatatt gccgcgcgga
     6721 tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg
     6781 ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat
     6841 gcctgggcgg cgttatagcc caccagaatc actaggaagg tgaacaaacc tttgctgaac
     6901 accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc
     6961 accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc
     7021 agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg
     7081 gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcctcg
     7141 cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc
     7201 tgcacctggt cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga
     7261 tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg
     7321 gcgatatttc ccggtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc
     7381 tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc
     7441 aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta
     7501 cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg
     7561 tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc
     7621 atcaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag catattgagc
     7681 accagtttct gcgctgtgcc tgctttcatc cgcgaagaac cggtaacaac ttcggcacct
     7741 acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg cgctccccgg attacaggaa
     7801 attcccactg tgcggcagcc gagctggcgt gcgtattcca gtccggcaat cacatacggc
     7861 gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg ccgttaaatt aatatttttc
     7921 agatcgttaa caccaccttc ccggctatct tcagcgcctt ccaccgcgtg ctgaatggca
     7981 tattcgcctc cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga
     8041 cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga
     8101 cgaccgccgc cgctgacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg
     8161 acgcgatcaa cggcaagcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc
     8221 agcgtcgata cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt
     8281 tctagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat
     8341 catatatccc gaattgacag acttaaagga atcattgagt ctacactcgc agagtgtgaa
     8401 gagcgtcata agaacttaag cggaattttt gctgccagcc caggagagtg gaagatgttg
     8461 tacctgacga aaatacgcaa tgcggaaagt gaatttaccg ggaacgagca aaaaattgct
     8521 gactttctgc gggccagagt gagcgagctg aaatccgtct cttcacggca gatggcgaaa
     8581 cagctcggta tcagccagtc gagcattgtg aaatttgccc agaagctcgg cgcgcaagga
     8641 tttactgaat tgcgtatggc gttaattggc gaatacagtg ccagccggga gaaaacgaat
     8701 gccacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc
     8761 cgcaaactga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac
     8821 gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc
     8881 ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat
     8941 cgcgtggcct gcgaagccga cacgcacgta caagccaccg tttcccaggc gttgaaaaaa
     9001 ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttgtgcgca
     9061 gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc tgactctccg
     9121 ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc
     9181 tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt
     9241 gtgcaacatc aatgatgtgg cgtcattgaa aatgattcag cgcagcagtg agctaacaca
     9301 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
     9361 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
     9421 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
     9481 gcattgcagg gaattggcga aggaattgcc agaacttttg cacgtcatgg cgcgaaccta
     9541 atcttgctgg atatctcccc tgagatcgaa aagctagcgg acgaactgtg tggtcgtggt
     9601 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
     9661 aaacgcgcga aggaaaaaga agggcgcatt gatatcctag tgaataacgc aggcgtttgt
     9721 cgtctgggca gtttcctcga tatgagcgat gaagatcgcg atttccatat tgatatcaat
     9781 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc gcgcaaagat
     9841 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
     9901 gcgtatgcct taacgaaagc ggcgattgtt ggcctgacta aatcgctggc ggtggagtac
     9961 gcgcaatccg gtattcgcgt taacgccatc tgcccgggat acgtccgcac gccaatggcg
    10021 gaaagcattg cccgccagtc gaacccggaa gatccagaat cggtgctgac tgaaatggca
    10081 aaagcaatcc cgctgtgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    10141 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    10201 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatgtg
    10261 tggggaggat ttcgtcttga actaagttca ccaggctatt tcatttgtca ttttggcccc
    10321 gggcagtgct cgaaatcctc acgtactatg tgtacgctcc ggtttctccg cgctgtccgt
    10381 gtccaaactg cctgcaacaa ttacgcctgt tgaaccaagt tcttattccc tttttaactt
    10441 ccaaatcacc aaacggtata taaaaccgtt actcctttca cgtccgttat aaatatgatg
    10501 gctattagaa agtcattaaa tttataaggg tgcgcaatgg ccgttaactt actgaaaaag
    10561 aactcactcg cgctggtcgc ttctctgctg ctggcgggcc atgtacaggc aacggaactg
    10621 ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg ccctgaatcc gccgtttgaa
    10681 cagcaatggg caaaagataa cggcggcgac aaactgacga taaaacaatc tcatgccggg
    10741 tcatcaaaac aggcgctggc gattttgcag ggcttaaaag cagacgttgt cacttataac
    10801 caggtgaccg acgtacaaat cctgcacgac aaaggcaagc tgatcccggc tgactggcag
    10861 tcgcgcctgc cgaataatag ctcgccgttc tactccacca tgggcttcct ggtgcgtaag
    10921 ggcaacccga agaatatcca cgactggaac gacctggtgc gctccgacgt gaagctgatt
    10981 ttcccgaacc cgaaaacgtc gggtaacgcg cgttatacct atctggcggc atggggcgca
    11041 gcggataaag ctgacggtgg cgatgaagcc aaaaccgaac agtttatgac ccagttcctg
    11101 aaaaacgttg aagtgttcga taccggcggt cgtggcgcaa ccaccacttt tgccgagcgt
    11161 ggcttgggcg atgtgctgat tagctttgaa tcggaagtga acaacatccg taaacagtat
    11221 gaagcacagg gctttgaagt ggtgatcccg aaaaccaata ttctggcgga attcccggtg
    11281 gcgtgggtcg ataaaaatgt gcaggccaac ggtacggaaa aagccgcaaa agcctacctg
    11341 aactggctct acagcccaca ggcgcagacc atcatcaccg actattacta ccgtgtgaat
    11401 aacccggaag tcatggacaa actgaaagat aaattcccgc agaccgaact gttccgcgtg
    11461 gaagacaaat ttggctcatg gccggaagtg atgaaaaccc acttcaccag cggcggcgag
    11521 ttagacaagc tgttagcggc ggggcgtaag taatgtttgc agtttcttca agacgcgtgc
    11581 tgccgggctt tactttaagc cttggcacca gtctgctgtt tgtgtgcctg atattgctgc
    11641 tgccgctctc tgcgctggtg atgcagcttt ctgagatgag ctgggcgcag tactgggagg
    11701 tgatcaccaa cccgcaggtg gtcgcggcct acaaagtgac gctgctgtcg gcgtttgtgg
    11761 catcgatttt taacggcgtt ttcggtctgc tgatggcgtg gattttaaca cgttatcgct
    11821 ttccgggccg cacgctgctt gatgcgctga tggatttacc ctttgcgctg ccaacggcag
    11881 tcgccggttt aacgctggcc tcgctctttt ccgtaaacgg tttttacggt gaatggctgg
    11941 cgaagtttga tatcaaagtc acctatacct ggcttggtat tgcggtggcg atggccttta
    12001 ccagcattcc gtttgtggtg cgtaccgtgc agccggtgct ggaagagtta ggcccggaat
    12061 atgaagaagc ggcggaaacg cttggtgcaa cgcgctggca gagtttctgc aaagtggtgc
    12121 tgccggagct ttctccggcg ctggtggcgg gcgtggcgct gtcgtttacc cgtagtcttg
    12181 gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc gtggaagacg gaagtgacgt
    12241 cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc ggcagcgagc gcgattgctt
    12301 cggtgatcct cgcggcatct ctgctgctgc tgttctcaat taacactctg caaagtcgct
    12361 ttggtcggcg tgtggtaggt cattaatggc ggaagttacc caattgaagc gttatgacgc
    12421 gcgcccgatt aactggggca aatggtttct gattggcatc gggatgctgg tttcggcgtt
    12481 catcctgctg gtgccgatga tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc
    12541 ggttttacag aatctggccg atccggacat gctgcacgcc atctggctga cggtgatgat
    12601 cgcgctgatt gccgtaccgg taaacctggt gttcggcatt ctgctggcct ggctggtgac
    12661 gcgctttaac ttcccgggac gccagttact gctgacgcta ctggacattc cgtttgccgt
    12721 atcgccggtg gttgccggtc tggtgtatct gctgttctac ggctccaacg gcccgctcgg
    12781 cggctggctg gatgaacata atctgcaaat tatgttctcc tggcccggaa tggtgctggt
    12841 caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg gtgccggtga tgttaagcca
    12901 gggcagccag gaagacgaag cggcgatttt gcttggcgcg tccggctggc agatgttccg
    12961 tcgcgtcaca ttgccgaata ttcgctgggc gctgctttac ggcgtggtgc tgaccaacgc
    13021 ccgcgcgatt ggcgagtttg gtgctgtgtc ggtggtttcc ggctcgattc gcggcgaaac
    13081 actttcgctg ccgttacaga ttgaattgtt ggagcaggac tacaacaccg tcggctcctt
    13141 taccgccgcc gcgctgttga cgctgatggc gattatcacc ctgtttttaa agagtatgtt
    13201 gcagtggcgc ctggagaatc aggaaaaacg cgcgcagcag gaggagcatc atgagcattg
    13261 agattgccaa tatcaagaag tcttttggtc gcacccaggt gctgaacgat atctcactgg
    13321 atattccttc tggtcagatg gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc
    13381 tgctgcggat tatcgccggg ctggagcatc aaaccagcgg gcatattcgc ttccacggca
    13441 ccgacgtgag tcgcctgcac gcgcgcgatc gtaaagtcgg tttcgtgttc cagcattacg
    13501 cgctgttccg ccatatgacg gtattcgaca atatcacttt tggcctgacg gtgctgccgc
    13561 gtcgcgagcg cccgaatgcc gcagccatca aagcgaaagt gacaaaattg ctggaaatgg
    13621 tccagcttgc gcatctggcg gatcgttatc cggcgcagct ttccggcggc cagaaacagc
    13681 gtgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat tctgctgctt gatgaaccgt
    13741 ttggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg
    13801 aagagctgaa attcaccagc gtgtttgtga cccacgacca ggaagaggcg accgaagtag
    13861 ccgatcgtgt agtggtgatg agtcagggga atattgaaca ggctgatgcg ccgaatcagg
    13921 tatggcgcga accagcgacc cgttttgtac tcgaatttat gggcgaagtg aaccgcctgc
    13981 agggaaccat tcgcggcggg cagttccatg ttggcgcgca tcgctggccg ctgggctaca
    14041 cacctgcgta tcagggtccg gtggatctct tcctgcgccc gtgggaagtg gatatcagcc
    14101 gccgtaccag tctcgattcg ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc
    14161 actacaccca attagtggtg cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga
    14221 tgcatggcga cgatgccccg cagcgtggcg agcgtttatt cgttggtctg caacatgcgc
    14281 ggctgtataa cggcgacgag cgtattgaaa cccgcgatga ggaacttgct ctcgcacaaa
    14341 gcgcctgata ggttgagtga atgttaaacg cccggaggcg cttcccgcga tccgggcttt
    14401 ttaatggcaa ggtttgtaac ctgtaggcct gataagacgc gcaagcgtcg catcaggcaa
    14461 caccacgtat ggatagagat cgtgagtaca ttagaacaaa caataggcaa tacgcctctg
    14521 gtgaagttgc agcgaatggg gccggataac ggcagtgaag tgtggttaaa actggaaggc
    14581 aataacccgg caggttcggt gaaagatcgt gcggcacttt cgatgatcgt cgaggcggaa
    14641 aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag ccaccagtgg taacaccggc
    14701 attgcgctgg caatgattgc cgcgctgaaa ggctatcgca tgaaattgct gatgcccgac
    14761 aacatgagcc aggaacgccg tgcggcgatg catgcttatg gtgcggaact gattcttgtc
    14821 accaaagagc agggcatgga aggtgcgcgc gatctggcgc tggagatggc gaatcgtggc
    14881 gaaggaaagc tgctcgatca gttcaataat cccgataacc cttatgcgca ttacaccacc
    14941 accgggccgg aaatctggca gcaaaccggc gggcgcatca ctcattttgt ctccagcatg
    15001 gggacgaccg gcactatcac cggcgtttca cgctttatgc gcgaacaatc caaaccggtg
    15061 accattgttg gcctgcaacc ggaagaaggc agcagtattc cgggcattcg ccgctggcct
    15121 gcggaatatc tgccggggat tttcaacgct gcgctggtgg atgaggtgct ggatattcat
    15181 cagcgcgatg cggaaaacac catgcgcgaa ctggcggtgc gggaaggaat attctgtggc
    15241 gtcagctcag gcggcgcggt tgctggagca ctgcgggtgg caaaagctaa ccctggtgcg
    15301 gtggtggtag cgatcatctg cgatcgtggc gatcgctacc tctccaccgg agtgtttggg
    15361 gaagagcatt ttagtcaggg ggcagggatt taaggattaa tagcattgga gactgatgac
    15421 aaacgcaaaa ctgcctgatg cgctccgctt atcaggccta caatgtttct gcaatatatt
    15481 gaattagcaa tatttttatg ccggatcagg cgtttacgcc gcatccggca taaacaaagt
    15541 gcactttgtc aataatctga tttgtcggca gttgttgctg ccgacaaatg tagtttttaa
    15601 ttttcgtgtg gaaaagaagt aaggtcatca atcatttttt gaagtattgg tgagtcctga
    15661 ccgtcacccc attgaagaat ttttgcggta agctgatgac gcgctagtgc attgagtccc
    15721 atctttttaa gtttatggga gattttaccc atgactttga tctgaccgaa tgcagtcgcg
    15781 taggtaatca tattgctctg gatagagctc atttcatcgg ctagtaattg cgcacgcaca
    15841 tcctcttcaa gaatactgtc catagcgatg tatttcatac cccacatagt ttcaattaac
    15901 atttgaggga aatcagcaca gtccagaacg gaggctattt tcaagttcgt ttagagcatc
    15961 agcaccttca tcgctaccaa aaggagattc ttcatcccag caacacatat aaagtggatc
    16021 agtaaaatgg cactggaagt ttgggtgtga cgttttaaga tgtaacccaa tctcttcatc
    16081 atcaaaatag tagagatcca tgaagttaaa gtctggagtt tcttgatatc cttttttcat
    16141 ttttgcggca atcaatttac tggcttcttt cagacactgt tcttcattat cgaattcttt
    16201 tgtatggaat ttaccaatac tacctacctt gccatagttg acagctaaac tatccccttc
    16261 gtaatctatc caccaaaatt tattcgattt ttcatcgtga taaataaagc attttttcat
    16321 gttacctcct gtaaatgcct taccaaagcg gattaatttc cttccgcagg caccgtttta
    16381 tccgtctgcg caattaacgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg
    16441 atcttcacga tatacggctt cccggtaatc gatgacgacg aagctacctt atcaataaac
    16501 tgctcggcag tgtcaatgcg gttacgggta ttgccgagct tcagacgcag gtgggaaacc
    16561 gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct
    16621 aatccctcca gcatggcgtt gatgcgggct tcttcatgaa cagttaactt cgcgtaagcg
    16681 gggagtgtca ttagcagcgt aatggccaga caaatgattt tcttcactga attaccttat
    16741 ctttgttttg taatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg
    16801 atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa
    16861 gagtagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtagtgt acggaagcgt
    16921 aggcaacagc attgcggtgc ctgctataaa acagaacggc ttgaatgtct ttgccgtgcc
    16981 gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga
    17041 cgaatggttt agcggctatt tgcgtgcgct tcaggagcgt gatgcgctgc gccaacttcg
    17101 tgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct
    17161 gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga
    17221 tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact
    17281 gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa
    17341 ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa
    17401 atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt
    17461 ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg
    17521 gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa
    17581 cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca
    17641 tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg
    17701 cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac
    17761 ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac
    17821 cggagtcagg gtagacttgg ctttctcttc cagcagcggc agatcaaatt caatgacagt
    17881 atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag
    17941 ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga
    18001 gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt
    18061 gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc
    18121 cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc
    18181 ggtatccttt ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt
    18241 ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga
    18301 acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta
    18361 acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa
    18421 tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat
    18481 cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa
    18541 cttgcttgat caagttcagc acggacggtg acattggctg gtaaaggtgt gaaatcatat
    18601 cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga
    18661 aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta
    18721 cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga
    18781 tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa
    18841 tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct
    18901 ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg
    18961 ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg
    19021 cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg
    19081 gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc
    19141 cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcat acttctacct
    19201 ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag
    19261 aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt
    19321 aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga
    19381 cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg
    19441 aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg
    19501 tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt
    19561 cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag
    19621 ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga
    19681 taacttcatg agcagctgcg tcagctgtca tgtgtttatc tttaatcagg gctatgattt
    19741 cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt
    19801 cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg
    19861 cacgaccgct cagaaaacgt tcaacttcct gagcaacctg gtcggcagaa atttttttcc
    19921 ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg
    19981 atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc
    20041 gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc
    20101 ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt
    20161 aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa
    20221 gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt
    20281 aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa
    20341 ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga
    20401 cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc
    20461 gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc
    20521 agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat
    20581 accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa
    20641 aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcaat
    20701 tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgat
    20761 ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgct
    20821 gcggcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc cattagacga
    20881 cgggcggtag aaatggcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga
    20941 tcgaggttag ccgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta
    21001 atctcttcac ctgccagcac ctgggcgata actggagaat cggttggctc aacggcgaca
    21061 gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctg
//
LOCUS       DADLTI010000064        21025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLTI010000064 DADLTI010000000
VERSION     DADLTI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.064"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..3086)
                     /locus_tag="JN755_002101"
                     /pseudo
     CDS             complement(1..3086)
                     /locus_tag="JN755_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3384..4577
                     /locus_tag="JN755_002102"
     CDS             3384..4577
                     /locus_tag="JN755_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7380897.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4775..6028)
                     /locus_tag="JN755_002103"
     CDS             complement(4775..6028)
                     /locus_tag="JN755_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7380898.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6355..7545
                     /gene="hmpA"
                     /locus_tag="JN755_002104"
     CDS             6355..7545
                     /gene="hmpA"
                     /locus_tag="JN755_002104"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7380899.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="JN755_002105"
     CDS             complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="JN755_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7380900.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="JN755_002106"
     CDS             complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="JN755_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7380901.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="JN755_002107"
     CDS             complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="JN755_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7380902.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="JN755_002108"
     CDS             complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="JN755_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7380903.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12176..16063)
                     /gene="purL"
                     /locus_tag="JN755_002109"
                     /gene_synonym="purI"
     CDS             complement(12176..16063)
                     /gene="purL"
                     /locus_tag="JN755_002109"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7380904.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16321..17877
                     /gene="mltF"
                     /locus_tag="JN755_002110"
     CDS             16321..17877
                     /gene="mltF"
                     /locus_tag="JN755_002110"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7380905.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="JN755_002111"
     CDS             complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="JN755_002111"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7380906.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="JN755_002112"
     CDS             complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="JN755_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7380907.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19279..20127
                     /locus_tag="JN755_002113"
     CDS             19279..20127
                     /locus_tag="JN755_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7380908.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20466..21020)
                     /gene="pinE"
                     /locus_tag="JN755_002114"
                     /pseudo
     CDS             complement(20466..21020)
                     /gene="pinE"
                     /locus_tag="JN755_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5052 a         5677 c         5353 g         4943 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccag agtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtcctga ctggcatacg ccaccgcttg cgggaaatct
     1741 gcgcggttat attccagcat cgccagcgcc aggttacagc gataatccag cgggtccagc
     1801 gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga
     1861 tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct
     1921 ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt cctgataact cagtacaata
     1981 ttgccgtcgg catcggagag ttcaatggtc agtctgtcag gattgatacc gtgcaacacg
     2041 ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc gttgcatttg
     2101 ccgatttcgc ggatcgccag acggtatccg ttcaacggag agatggcata cagcccccac
     2161 tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc
     2221 atgcacagag aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca
     2281 agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg
     2341 ccgttattgt cggtcagact cttatcccac gcctggccaa attcactgtg tccccaactc
     2401 cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca
     2461 tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt cggccatata tgaggttgga
     2521 acaggcacat ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa
     2581 gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc
     2641 acatccggtg ggaaaacgct ctgatgccct tcaccccctt tcactgccgg gttggcccac
     2701 cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc
     2761 gcacggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt
     2821 tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc
     2881 ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc ggagatccac
     2941 ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgc
     3001 tgtttcactt tatcccatgc gcgatgcacc cgaccgccca gttccggcag gatcatcact
     3061 ttgatgtagt cgttttccag ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc
     3121 agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt
     3181 tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc
     3241 tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta
     3301 aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat
     3361 taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc
     3421 ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc
     3481 tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa
     3541 gcgaaaaacg ggtggtgaat attgacgatg aaagccagac gcgcgggcat agtagcggta
     3601 catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta
     3661 ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag tatttacaga
     3721 ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc
     3781 gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggg caggttgatc
     3841 cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca gttgaggtga
     3901 agtatctgct ggaagagaag ctgggcattc gggtgctggt cgataatgac tgcgtgatgc
     3961 tggcgcaggc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg
     4021 ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt
     4081 atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg
     4141 gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg
     4201 tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg acaaccgcgc
     4261 agttaatcgc tgcctggcag agtggagaac cgtggatcac cagctgggtt gatcgctctg
     4321 ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt
     4381 ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga
     4441 caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct
     4501 tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc
     4561 agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg
     4621 atcataaacg aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg agattgcaat
     4681 atattgaatt tgcaagattt tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa
     4741 caacgagcac attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc
     4801 agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca
     4861 gcacgtcaca catccagcca gccagttctt tcgcttcagc ttctttaaag ccgcgacggg
     4921 taatcgccgg agtacctaca cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
     4981 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
     5041 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
     5101 ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct
     5161 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg
     5221 cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
     5281 tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg
     5341 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
     5401 cagcaaccag gcccgcaacg tgcgccatat caacgaacag ataagcaccg atgctgtcag
     5461 cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa
     5521 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg
     5581 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
     5641 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
     5701 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
     5761 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
     5821 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
     5881 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
     5941 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
     6001 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
     6061 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
     6121 gataacaggt cttgacaaag gaatttacgc aaacgattac cttaaggcta cgcaaggctt
     6181 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
     6241 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
     6301 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaggaaga ccatatgctt
     6361 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
     6421 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
     6481 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
     6541 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
     6601 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
     6661 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
     6721 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
     6781 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
     6841 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
     6901 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
     6961 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa
     7021 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
     7081 gtgaaactgg tcgctccagc aggtgatttc tttatggctg tcgcagatga cacaccagtg
     7141 acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
     7201 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac
     7261 gcctttgctg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
     7321 tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
     7381 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
     7441 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
     7501 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
     7561 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
     7621 cggatgcgaa tgacccgagc cacgtcaaag acgaagattt taccgtcacc gattttgccg
     7681 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
     7741 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
     7801 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
     7861 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
     7921 tttttcatgc tattccttga aaaggttgtc tgtcttttga tctgctaaac gtaacacata
     7981 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
     8041 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt
     8101 tgcagcagct tacgcaaata gttgagttca aaatgattac gtgcctcaac aaaggttggc
     8161 agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat
     8221 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
     8281 ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt
     8341 cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc
     8401 gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
     8461 tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg cagaaataat ccgcacattg
     8521 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
     8581 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
     8641 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
     8701 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
     8761 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
     8821 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
     8881 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
     8941 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg
     9001 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
     9061 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
     9121 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
     9181 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
     9241 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
     9301 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
     9361 actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg
     9421 ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
     9481 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
     9541 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
     9601 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
     9661 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
     9721 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
     9781 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
     9841 cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat
     9901 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
     9961 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    10021 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    10081 acatggacag cgcggagaaa ccggggcgta catatcgtac gtgaagattt cgagcactac
    10141 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    10201 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    10261 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    10321 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    10381 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    10441 tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    10501 taaagattat ccagtacgct catcagcagc attggctccg ccaagcaagc tgttgctttg
    10561 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    10621 gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta
    10681 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga
    10741 atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc agccagtaat
    10801 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    10861 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    10921 ccaaccgagc gtaactcgct cggtccacag aacgaaacgc tattgcccag agaacgccct
    10981 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    11041 aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    11101 tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc
    11161 gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca
    11221 gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat
    11281 gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa
    11341 cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga
    11401 cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca
    11461 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    11521 gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt
    11581 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    11641 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    11701 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    11761 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca
    11821 cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac
    11881 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gagagattta
    11941 tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa
    12001 gataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg
    12061 taaaccaccc attgtcgtga tttacagaca caaaaaagct cccagagttg ggagcttatg
    12121 atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    12181 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    12241 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    12301 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    12361 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    12421 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    12481 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    12541 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    12601 gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    12661 atacccccag ggccagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    12721 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    12781 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    12841 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    12901 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    12961 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    13021 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    13081 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    13141 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    13201 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    13261 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    13321 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    13381 ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    13441 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    13501 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    13561 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    13621 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    13681 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    13741 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    13801 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    13861 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    13921 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    13981 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    14041 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    14101 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    14161 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    14221 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    14281 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    14341 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    14401 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    14461 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    14521 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    14581 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    14641 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    14701 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    14761 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    14821 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    14881 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    14941 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc ggtaacaatg cgctcaggct
    15001 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    15061 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    15121 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    15181 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    15241 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    15301 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    15361 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    15421 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    15481 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca agacgcaggt
    15541 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    15601 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    15661 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    15721 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    15781 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    15841 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    15901 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    15961 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    16021 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    16081 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    16141 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    16201 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    16261 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    16321 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    16381 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    16441 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    16501 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    16561 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    16621 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    16681 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    16741 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggtg
    16801 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    16861 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    16921 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    16981 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    17041 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    17101 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    17161 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    17221 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    17281 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    17341 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    17401 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    17461 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    17521 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    17581 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    17641 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    17701 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    17761 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    17821 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    17881 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    17941 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    18001 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    18061 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    18121 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    18181 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    18241 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    18301 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    18361 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    18421 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    18481 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    18541 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    18601 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    18661 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    18721 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    18781 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    18841 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    18901 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    18961 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    19021 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    19081 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    19141 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    19201 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    19261 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    19321 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    19381 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    19441 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    19501 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    19561 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    19621 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    19681 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    19741 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    19801 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    19861 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    19921 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    19981 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    20041 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    20101 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    20161 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    20221 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    20281 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    20341 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    20401 gtgacataca acattaactt ttcagtcaac cgtagcacgt ccttgtatac gccgtgtctg
    20461 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    20521 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    20581 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    20641 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    20701 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    20761 aagttgatgc ctcgttcgcg caactcctcc accagcacga caagatgccg catactgcgc
    20821 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cctgagcagt
    20881 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    20941 catcctgcac agttcaacgc attacgttgt agatcggtat tctggtcatt tgttgatacg
    21001 cgtacatagc caataagcat ggtaa
//
LOCUS       DADLTI010000065        20619 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLTI010000065 DADLTI010000000
VERSION     DADLTI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.065"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..848
                     /locus_tag="JN755_002115"
     CDS             <1..848
                     /locus_tag="JN755_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7380909.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
     gene            complement(886..1734)
                     /locus_tag="JN755_002116"
                     /pseudo
     CDS             complement(886..1734)
                     /locus_tag="JN755_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(1756..3768)
                     /locus_tag="JN755_002117"
                     /pseudo
     CDS             complement(1756..3768)
                     /locus_tag="JN755_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            complement(3798..4211)
                     /gene="hyfJ"
                     /locus_tag="JN755_002118"
     CDS             complement(3798..4211)
                     /gene="hyfJ"
                     /locus_tag="JN755_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY7380910.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(4204..4962)
                     /gene="hyfI"
                     /locus_tag="JN755_002119"
     CDS             complement(4204..4962)
                     /gene="hyfI"
                     /locus_tag="JN755_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7380911.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4959..5504)
                     /gene="hyfH"
                     /locus_tag="JN755_002120"
     CDS             complement(4959..5504)
                     /gene="hyfH"
                     /locus_tag="JN755_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7380912.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(5514..7228)
                     /gene="hyfG"
                     /locus_tag="JN755_002121"
                     /pseudo
     CDS             complement(5514..7228)
                     /gene="hyfG"
                     /locus_tag="JN755_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(7218..8798)
                     /gene="hyfF"
                     /locus_tag="JN755_002122"
     CDS             complement(7218..8798)
                     /gene="hyfF"
                     /locus_tag="JN755_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7380913.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8803..9453)
                     /gene="hyfE"
                     /locus_tag="JN755_002123"
     CDS             complement(8803..9453)
                     /gene="hyfE"
                     /locus_tag="JN755_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7380914.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(9465..10904)
                     /locus_tag="JN755_002124"
     CDS             complement(9465..10904)
                     /locus_tag="JN755_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7380915.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10932..11873)
                     /gene="hyfC"
                     /locus_tag="JN755_002125"
     CDS             complement(10932..11873)
                     /gene="hyfC"
                     /locus_tag="JN755_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7380916.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN755_002126"
                     /pseudo
     CDS             complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN755_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN755_002127"
     CDS             complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN755_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7380917.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN755_002128"
     CDS             complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN755_002128"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7380918.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15241..15813)
                     /locus_tag="JN755_002129"
     CDS             complement(15241..15813)
                     /locus_tag="JN755_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7380919.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15959..16837
                     /gene="dapA"
                     /locus_tag="JN755_002130"
     CDS             15959..16837
                     /gene="dapA"
                     /locus_tag="JN755_002130"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7380920.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16854..17888
                     /gene="bamC"
                     /locus_tag="JN755_002131"
     CDS             16854..17888
                     /gene="bamC"
                     /locus_tag="JN755_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7380921.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18101..18814
                     /gene="purC"
                     /locus_tag="JN755_002132"
     CDS             18101..18814
                     /gene="purC"
                     /locus_tag="JN755_002132"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7380922.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18982..19845
                     /locus_tag="JN755_002133"
     CDS             18982..19845
                     /locus_tag="JN755_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7380923.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19860..>20619
                     /locus_tag="JN755_002134"
     CDS             19860..>20619
                     /locus_tag="JN755_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7380924.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASD"
BASE COUNT         5183 a         5470 c         5546 g         4420 t
ORIGIN      
        1 ttgtattggt ggtttttgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg
       61 cctggcaaca gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg
      121 actttgccgc cacgttgccg cgccgctatc cggtgttaat ggacgcgggc attattgatg
      181 caatggccga aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt
      241 ccctcgcctc gctggtcggt ttgctgacca tagccgtcta tctggtgctg gtgccattga
      301 tggtcttctt cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc
      361 cgcgtaaccg tgggctggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact
      421 atatccgcgg caaagtgctg gagatgatcg tggtggggat cgccacctgg ctggggttct
      481 tgctcttcgg gctgaactat tcgctgctgc tggcagtgct ggtcggcttc tcggttctta
      541 ttccgtacat tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt
      601 ttggcgcagg tactgaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg
      661 acggcaacct gctagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga
      721 ttattttatc ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca
      781 ttccattggc gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatcgcgc
      841 aagaataatc ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg
      901 taaataaata gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag
      961 catcaccggg agcagattgg cggtaataaa atggctgacc gtcagtgccg gaaaattgtc
     1021 tgcgctactg tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc
     1081 gaacggaatc acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt
     1141 gatggacaaa atcatggcga cgattttgtc gcataacgaa cgtccgcaat aactcatcca
     1201 cagcgccagg caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt
     1261 atgatgcatt ttgccctcgg cgcagtgtaa aatcgcgact ccccacatcg cgttttcact
     1321 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
     1381 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
     1441 tgccattacc gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat
     1501 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctatg aagacggggc
     1561 atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
     1621 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
     1681 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
     1741 tcctgcaaaa gcagattaca acacctcgcg aaccgagatt cccagacgct gcattcgtga
     1801 cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat
     1861 gccattggtt tctcgcaata cctgaacaat gcgctgacgt tcttcttcgt cattctctgg
     1921 cgtcgtcggg tgcagcaact gcgccattga actgcgaagc gctgaatctt catttagcgt
     1981 cggcagtaaa cggctttgtc ggacattaag atgtaaattc agactgttac cgcgagtcag
     2041 cagtaccgct cgctcaatca cgttttccag ctcgcgcacg ttgcccggcc agtcccacga
     2101 catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt gaacgattca tatggcgcgc
     2161 cattttttgc gtgaaatgct ttgctaaaag agggatatct tccggacggt cgcgcagcgg
     2221 aggcaattcc agtgggaaga cattcaggcg ataaaagaga tcgctgcgaa actggcgatc
     2281 ttcaaccatt tgccacaaat cacggttggt ggcggcaatg actctgacat ttaccgggat
     2341 cgttctactc ccgccgagac gctcaatctc ccgttcctgc aatacgcgca gcagtttagg
     2401 ctgaagttct aacggcagat cgccaatttc atcgagaaac aacgtgccgc catcggcaat
     2461 ttcaaaacga ccacgatggg tattaatcgc accagtaaac gcccctttgt catgaccgaa
     2521 taactcactt tccagaagac tggcggggat ggcagcgcag ttgattttga ccagcggctt
     2581 gtcgtgtcgc gggctaagtt gatggatcgc tctggcgatc acctctttgc cggttccggt
     2641 ttcaccgcaa atcaacaccg tactgtcgct cttcgccaca atatctacct gctggagcag
     2701 gtcttccatt gcctggcttt gatagataat gtcaccgacg cccagattcg aaaggagctg
     2761 ctcgctaagc tggtggtttt cttgctgcaa actttcctgc aaatcggtca ttctacggca
     2821 ggcatcggca ttgccaacgg caatagcgat gcgatcggct atgtgttgta gtagctggca
     2881 gttttcctca ctaaagagag tggaagaggt atgcgccagc aacaatgcac ccggtgtatg
     2941 gttgccaaag gtaagcggta taaggagcgc agattcgcag ccgttagatg caagtaagag
     3001 taataacggg tcgcgttgcc agagaaacag atcatctgca cggtgcgtca aggtcggttg
     3061 tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca ggcatattgt gtggcagaca
     3121 cgcacaatgt gaggcagaaa gatcgctgca ccacaggctg aacttctcgt tctttcgatg
     3181 atcgcccagt accatactga ctgaagccag accgaaaaaa tgatggatct cacgagcgac
     3241 gtcagcgatc agatcgtcga gatcaagatg tgacagcact gcattagtga tatccaccag
     3301 tacccgatga tgatcgcgat ctttctccag caaatgcagc gccacctgct gttcttcatc
     3361 attgagccat gattgcagaa ccggggtgat aatgcgtgcc agccagttaa acagtgtgca
     3421 atcctgggga gtaaaacggc tgccgtgggt acgaataaag cgtagctccg ccattcaact
     3481 actgccgaga aggacggcgt gacgcgaata atcgaccatc tcgccctgat actctgttgt
     3541 tgccgtcaca tcgctgccat catcatggta gcggtaatag aagttgcttt tgagaacatg
     3601 cagttcaata agttgtaccg ggctgaacgg cggcggcagc gggatgaagg cgcgtaataa
     3661 agacgccttg ccgtgtttct gcgctaaccg ctccgcgagc attccgttta ccgcttcaat
     3721 tgttattcct tgcggcgggg caaacatcgc ttcgtctgac atagccatca acagtcctta
     3781 ttaatgagaa atccttctca cgcaattttt ctggccatca gatagatagc cggttccagc
     3841 acaatttcat ccaacagatg aatgaattga atgctacgcg cttgccacac agacgtcgcg
     3901 tcatctgcca gtggcgcgaa tgcccgggtc aacagggcgg tgtggctggc gtcaataaca
     3961 atctcaccaa aagtcatcac ccccagcatc ttacgtcggg cttgctcctc ttcgaccagt
     4021 gcaagccaat cttcgtattc cgtcagtggg cagcggaagg cgacattcag acaatcaatc
     4081 acgccaacgt gatgaccgat agccagcgag taatacatta cctgagagct gtgttccggc
     4141 atctcgtcgc tactggcgac aaactttttt cgcagcgtcc agaaaacaat ttccccgcac
     4201 tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc tgaacgatac tattgagacg
     4261 tggatcgtcg gcatcgcgca accaggtgtt aacccgattt cctgtaggat cgtcgcttaa
     4321 atggcgcagg agccgatcgc aaatttctcg cccctgacga tagcccgcca gccgccgcgc
     4381 ttctcgctca atggcgatac gctgtgatgg cgggatctgc ggccacaacg gttgcatagt
     4441 caccccggtg ggatcgcgat aatccaccgc gtgaatcttc tgttgcagca aaccgagcgc
     4501 cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg cagccgggga tccaaacatc
     4561 aatggggaca atggtgtcgc tgccgcccca gacgctgtag agatcgtgga aaataccgcc
     4621 gccgacaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc
     4681 ccgaagtgca ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc
     4741 gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata cgggggtgat
     4801 ggcagcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgatgc gataaacgta
     4861 agccgagcga cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag
     4921 cgtgatgggc tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc
     4981 agcggtacat ctgtatcgtc gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg
     5041 ctcgcttgcg cccataacat ttcgcggttt tgtggagcat tttgttgctg tgccaacaat
     5101 tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt
     5161 tgtagatgga acgtcgcgtg ggtatagaga tcggctttat gggtgacggt cagttcaaag
     5221 ttattggtaa gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata
     5281 caacgcccca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc
     5341 agcgcatttg ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc
     5401 aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc
     5461 gtcggggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc
     5521 agcggcgaac ggttacgatc aatgccgtag cgttcgatct ctttatacag cacggttttt
     5581 gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca
     5641 aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac
     5701 accggccagt tggcgtaggt ggcggcacga cagcgccagc ggaacaattt ttggttatca
     5761 ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat
     5821 gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg
     5881 gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag
     5941 aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag
     6001 gggtgatcaa agcgcaaatc acgggcaaaa ccactgcgcg gatcagcggc cctacagggc
     6061 tataatcacg ggcgatttgt cggtcgagaa tgccaatgcc ctgagtgcgt tgttccatat
     6121 tcggcgtagc aagcagcatc tctaccagct ccgacacgtc ggagcgcatc tcgcgcacca
     6181 gtttcagcgt ttgcagacgt tgctctttga gaatatcgcg gcgaacacca ccaatcagat
     6241 tcagaccgta ggttttacgc gacccggtca gcaattccgc catcgtcatc gacttttccc
     6301 gcacgcggaa aaattgcata aagccggtat caaaaccaac aaaatggcag gagaggccaa
     6361 ggttgagcaa atgactgtgt agccgctcga cttccagcag aatcgagcga atagtatgtg
     6421 ctcgttgcgg cacctcaatc cccagtgcat tttcaaccga gttggtatag gccacactgt
     6481 gggcaaaacc gcaaatccca cacacgcggt ccgataagaa ggtcacttcg ttgtagccca
     6541 tccgcgtttc tgccagtttc tccatgccgc gatggacata aaacaggcgg taatcagcat
     6601 cgacaatttg ctcgccatcc acaaacaagc ggaagtgacc cggttcatag gaggtgatat
     6661 gcagcgggcc gacagggatc acccgcgcat cgctgttgcc ctcattgata aacgcatacg
     6721 tttcggaatc tgtcgtcggt tcagggcgca gtcgataatc catcgcatct ttgcgcagcg
     6781 gatgcatatc ttccggccag tcatcgggca acaccaggcg acgctgatcc ggcaggccaa
     6841 ccggaatcag cccgtacata tcgcgaattt ctcgctcgcc ccagaccgcg gcagggacgc
     6901 gcggtgtgac tgacggaaac tcccgactgt cggcatcgac cagtgccttc accacgatcc
     6961 agcatttttc ggccccttcc attgaaaggg cataataaac cgcgtaatga ccgttaagtg
     7021 tccgctcgtc gttgccaaac aggactggaa gccagccatc atgttgataa taaagataat
     7081 gtacaacgtc aggcagcaga ttgattttca ccgtaatggt gacctgttca ggcgtttgtc
     7141 gctcttcatc cagcaccgcg ccggggaact gcgttttcag ggcggcgaga atcgcttcgc
     7201 cacgatttga cgatgaatta acgttcactg tgtttctccg aaacagatgc ggtgcctgaa
     7261 ggcaaaaaat catgccaggt gctacgttgt gcaggcaggt cgtgcgtccc tgagaggact
     7321 atagtggaag cgcccgccag gatcctgatg acaggttgtg gaatatgcgt tcccatcgcc
     7381 agcatcatga ctagcagaat caccattggc gaggtggtca accagccgag atcaccccgg
     7441 ttaacggcct gcggcggttt cgccattaac acccgcgcag ccatccgtac caggcccgcg
     7501 agcaccagcg ttaacagcaa taacagcagg acgataatca gcaggtgatt acgtgccagt
     7561 cctgcggtaa cggtcataaa ttcgctaaga aaaatgttga agggcggcat ccctgccagc
     7621 gccagcgcac cgccgccaaa cagcacggcg gtaaatggca tgattttgag catcccacag
     7681 acgacgttga gatcgcgcgt gccgtacttg agcagtacat tgccggaacc gcagaacagc
     7741 agcgtttttg ccagactgtg gtttaaggtg tgcagcagcg cggcaaaaat tcccagcggc
     7801 ccgccaatgc ctagcgccac cgcgaccagc cccatgttct ccacgctgga gtacgccagc
     7861 agacgcttaa tgtcccgctg taccagaatg aaaaatgccg ccacggcaac cgacaacatg
     7921 ccgaagatga gcaacaaccg gttggggaaa tcgctgccga tggcctggca aataatgatg
     7981 taatagcgaa tcagcaccaa cagcgcgcag ttcagcaata cggcggagag cagggcgctg
     8041 accggactcg gcgcttcact gtgagcatcc ggcagccagg cgtgcatggg aaatagcccg
     8101 gttttggtac caaagccaat tagcacaaac acaaaggcca acagcattaa tgttgggtca
     8161 agcaaggacg attgcttaag aacctcgctc cagaatatcg ccatttctgc ctgcggcata
     8221 acgctggcgg cgttggcgta taccagcacg gtaccgaaca gaccaaaagc gacaccaaca
     8281 gtacaaataa tgatgtactt ccatgctgat tccagcgatg aacgctgacc gtaaatgcct
     8341 accagaaacg ccgagcttaa ggtggtggct tcgatcgccg cccacatcac aatcaggtta
     8401 ttgctggtaa caaccagcag catggtgaaa aaaaacagat ggaagaaacc gtagtaatcg
     8461 cacagtgtaa cgggtgaaag ctcgccgtgt gccacttcat gacgcatgta gccaatcgag
     8521 taaacaccgg tgagaaagcc aatcacacca agaatggcga ggaacaaacc gcccagacca
     8581 tcaatatgaa gccacagtcc cgcagcgaat atttctcctg catcagcggc agtttggacc
     8641 acccagagtg ccagaatcag cagcagtgtg atccctaagc tatgtaatac agtcaccgta
     8701 caagtcgcag agagcccccg tttccggcag gcaaaacaga gcagcgaaaa aagcagcggc
     8761 gtgagcagga gtaaagcgaa catcacagaa taactcatct cattatccct tcagcgcggt
     8821 caagttgttc acgtccagcg tgccgtgggt acgccatatt tttcttgcca gtaacaccat
     8881 cacaatgacg gcgaagatgg cgtcggtagc gatacctatt tccaccagtt ccggtgctcg
     8941 ccaggcaaga agcgccagca ccagatggga gccgttttcc atcaggcagt aaccaaaaat
     9001 ttgccgcagg atattgcgct ggctgacaat gcacagcagg ccaagcagaa aatgacctaa
     9061 cgctaccgcc agcgccggtt tcagcccggt agccatcggt agcttcacgg gctgaacgac
     9121 aaatgcgcaa agcaggacaa ttaacgccgc gagcagtgcc atcattgccg gaccgaataa
     9181 cgctttttcc gggatgttct ggggaatatt tcgtgcagcg taagtcatga ttaacggtac
     9241 cagcagcact ttggtgataa aggcgctggc ggaccagatc agcagttgct ctgcggcgaa
     9301 caggcacgag agagtggcga aaatagacac cagcaccagc gactggcagg cgtaaaatcc
     9361 gcatgacagg cgatagcttt tgacgctaat cacaaacagc gatgtcagca tcatcagtcc
     9421 cgccagatta tttacgatca tagaaccggt cattcatcat ctccttactg caaccaggtc
     9481 gcggcgatta cgctggaaat cagcgacatc acaatcagta caatcaacac caggcgcatt
     9541 gaccctggca gcggtgcggc atcggcgacg gcctcgctcg gtttgcccgg cacgacgcga
     9601 ccaaaccagc gaataaacca ggcgaaactg gcgaccgatt caatcatcag cagaatcatg
     9661 gcgggcagca ggatccagta ctccactgac aacgcaaaac cggcggcaaa cagcgggaat
     9721 ttactgaaga agccgttgaa cggcggcaca ccggtaattg ccagcgctgc cacgcagaaa
     9781 cccacgcctg gcaacggcag ggtgtgcaat acgccacgca gacgcggcaa caagcgcgtg
     9841 ccgcagctgt aactcagcgc acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg
     9901 ttgacgatgt aggcgatgct accctccagc gccagccgcg agccgaagat ggagagcgac
     9961 aagccgaaga acatccagcc aagttgagtg atggtcgacc aggccagcaa ccgcttcata
    10021 tcctgctgtg gcaaatacat cagaaagcca taaagaatgg tgaccagtgc catgaccatg
    10081 ccaacgccgc caatcacatg cgggatattg ccgccgtcga taatggcgcg ggcaaaaatg
    10141 taaacgccca ctttcaccat cgatgcggcg tggagatagt cgctgatcgg tgttggcgct
    10201 tccattgcgt ccggtagcca cgcttgcatc ggtagctggg ccgatttccc ccacgcggca
    10261 aacaggatgc cgccataaac cagataacgt gcgtcgccat gtaactcgct catcgcgcta
    10321 agcacaaacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa ccccaacgaa
    10381 ccgatatggg tgataagcaa cgcttttagt gctgaacgct gcgctttatc gctctggtaa
    10441 taactgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa ctgaccgagc
    10501 agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa aaatgcgtaa
    10561 taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata acccgtggag
    10621 tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt actcacgcga
    10681 tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa cgtgacggca
    10741 actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt ggtcagcgcg
    10801 gcgaacaaaa ccccccattc ggcggcccga cgttgtggcg aaaacgaaac gaccagtgcg
    10861 ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg tcagtgctcc
    10921 ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc caagccaagc
    10981 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    11041 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    11101 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    11161 atcacgacct gtttcagccc ctgcccccat ttcgctagcg ccagcccggc accggaatat
    11221 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    11281 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    11341 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    11401 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    11461 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    11521 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    11581 ggggaaacgg taataaatag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    11641 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    11701 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    11761 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    11821 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    11881 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    11941 gagacgaaag tcgccgcttt gcagatgctg tatttctttg accagccgtt gggtggcgct
    12001 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    12061 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    12121 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gagccatcgc
    12181 gttttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    12241 cgaacgacac cacagccagt aaaggcctgg cagcaaaggc atcgccagta gtaacagcaa
    12301 cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    12361 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    12421 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    12481 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    12541 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    12601 taccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    12661 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    12721 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    12781 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgtccgcgc cgagaaatag
    12841 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaactac
    12901 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgactg ccagcaaaat
    12961 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    13021 cgcatatagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    13081 ccacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    13141 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    13201 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    13261 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    13321 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    13381 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    13441 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    13501 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    13561 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    13621 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    13681 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    13741 aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt gatgccggaa
    13801 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    13861 tagaggatca gcgaccaggt taataattgc agggcatcca ttagtgttgc tcctgagtga
    13921 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    13981 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    14041 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    14101 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    14161 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    14221 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    14281 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    14341 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgattgtt gacgtcttcg
    14401 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    14461 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    14521 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaaggg tgccattctg
    14581 attaactcaa tctattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    14641 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    14701 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    14761 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    14821 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    14881 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    14941 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    15001 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    15061 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    15121 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    15181 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    15241 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    15301 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    15361 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    15421 ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    15481 gcgttcaatt aaatgcgggg agtctgcaac atcgacctga acccagacag atgctggcat
    15541 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc
    15601 tttcaacggt aacgttgatt caatcagagt gatggcattc catgaaccgg aaagcagcat
    15661 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    15721 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    15781 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    15841 gttcttaagg aaagcataaa aaaatcatgc atacaacaat cagaacggtt ctgtctgctt
    15901 gcttttaatg ccataccaaa cgtaccattg aggcacttgt ttgcacagag gatggcccat
    15961 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    16021 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    16081 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    16141 gatgacgctg gatctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    16201 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    16261 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    16321 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    16381 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    16441 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    16501 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    16561 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    16621 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacataa
    16681 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    16741 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    16801 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    16861 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    16921 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    16981 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    17041 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    17101 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    17161 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    17221 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    17281 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    17341 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    17401 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    17461 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    17521 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    17581 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcgtcgctg gaaaaagtgg
    17641 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    17701 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    17761 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    17821 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    17881 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    17941 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    18001 gtagttcgga taaggcgttc acgccgcatc cgaaaaaaca tccggcacac cagacagcaa
    18061 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    18121 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    18181 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    18241 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    18301 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    18361 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    18421 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    18481 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    18541 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    18601 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    18661 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    18721 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    18781 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    18841 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    18901 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    18961 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    19021 gacaggcgca acagctctgg cggtccgtca atgggcggtc ccggtttccg tctgccgagc
    19081 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    19141 ttaaccgggt tgatgaccgg gcagccggtt tctcaacaac aatcaacgcg gtcaattagc
    19201 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    19261 tggggacaac agttcgaaaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    19321 cgtggaatga cgcgcaccgg ctgcggggcg ggccagtcca ttatggggcc gttctattgc
    19381 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    19441 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    19501 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    19561 gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    19621 gggcatagta tgcagcagca aggcgttctg gaaactggcg atctggaaga ggcgctgaat
    19681 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    19741 gacagtttca cacatggcac ttctcagcaa cgctgcagct ggtttaaacg tggtttcgac
    19801 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaatttcc ggggcaggga
    19861 tggctgaact gactgcgctt tacacattaa cagcgcaaat gaaacgtgaa gggatctgcc
    19921 gcttgctggt gttgagcgga gaagagggtt ggtgttttga tcatgctctt aagttgcgtg
    19981 atgccttacc tggcgactgg ctgtggattt cgccgcagct agatgctgaa aaccactgtt
    20041 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    20101 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    20161 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    20221 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    20281 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    20341 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    20401 agcaactctt acagcagcta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    20461 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    20521 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    20581 ttcgctttat tgcaccggat gccttgttag ccagcgatg
//
LOCUS       DADLTI010000066        20548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLTI010000066 DADLTI010000000
VERSION     DADLTI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.066"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN755_002135"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN755_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7380925.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN755_002136"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN755_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7380926.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN755_002137"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN755_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7380927.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN755_002138"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN755_002138"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7380928.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN755_002139"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN755_002139"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7380929.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="JN755_002140"
     CDS             4448..4813
                     /locus_tag="JN755_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7380930.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="JN755_002141"
     CDS             4963..5481
                     /locus_tag="JN755_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7380931.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="JN755_002142"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="JN755_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="JN755_002143"
     CDS             6712..7194
                     /locus_tag="JN755_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7380932.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JN755_002144"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JN755_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7380933.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JN755_002145"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JN755_002145"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7380934.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JN755_002146"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JN755_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7380935.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JN755_002147"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JN755_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7380936.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JN755_002148"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JN755_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7380937.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10863..11729
                     /locus_tag="JN755_002149"
     CDS             10863..11729
                     /locus_tag="JN755_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7380938.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="JN755_002150"
     CDS             11750..13036
                     /locus_tag="JN755_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7380939.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JN755_002151"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JN755_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7380940.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="JN755_002152"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="JN755_002152"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7380941.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="JN755_002153"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="JN755_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7380942.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="JN755_002154"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="JN755_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7380943.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="JN755_002155"
     CDS             complement(16984..17274)
                     /locus_tag="JN755_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7380944.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JN755_002156"
     CDS             complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JN755_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7380945.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="JN755_002157"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="JN755_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7380946.1"
                     /translation="MTKKKAHKPSSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="JN755_002158"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="JN755_002158"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="JN755_002159"
     CDS             19136..20377
                     /locus_tag="JN755_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7380947.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5368 c         4821 g         5054 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctag ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatggcg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgatccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DADLTI010000067        20303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLTI010000067 DADLTI010000000
VERSION     DADLTI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.067"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JN755_002160"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JN755_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7380948.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JN755_002161"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JN755_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7380949.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JN755_002162"
     CDS             complement(977..1267)
                     /locus_tag="JN755_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7380950.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JN755_002163"
     CDS             1701..2411
                     /locus_tag="JN755_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7380951.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JN755_002164"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JN755_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7380952.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JN755_002165"
     CDS             complement(3539..4198)
                     /locus_tag="JN755_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7380953.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /locus_tag="JN755_002166"
     CDS             4351..6684
                     /locus_tag="JN755_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7380954.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JN755_002167"
     CDS             complement(6653..7093)
                     /locus_tag="JN755_002167"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7380955.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JN755_002168"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JN755_002168"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7380956.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JN755_002169"
     CDS             7811..8509
                     /locus_tag="JN755_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7380957.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JN755_002170"
     CDS             8738..9946
                     /locus_tag="JN755_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7380958.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JN755_002171"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JN755_002171"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7380959.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JN755_002172"
     tRNA            12053..12129
                     /locus_tag="JN755_002172"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JN755_002173"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JN755_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7380960.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JN755_002174"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JN755_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7380961.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JN755_002175"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JN755_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7380962.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JN755_002176"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JN755_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7380963.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JN755_002177"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JN755_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7380964.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JN755_002178"
     CDS             complement(18744..20015)
                     /locus_tag="JN755_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7380965.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4860 a         5027 c         5329 g         5087 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatagcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aatcggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcg
//
LOCUS       DADLTI010000068        19916 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLTI010000068 DADLTI010000000
VERSION     DADLTI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.068"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..746)
                     /locus_tag="JN755_002179"
     CDS             complement(<1..746)
                     /locus_tag="JN755_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7380966.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            840..1037
                     /locus_tag="JN755_002180"
     CDS             840..1037
                     /locus_tag="JN755_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7380967.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1131..1319
                     /locus_tag="JN755_002181"
     CDS             1131..1319
                     /locus_tag="JN755_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7380968.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1240..1965
                     /locus_tag="JN755_002182"
     CDS             1240..1965
                     /locus_tag="JN755_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY7380969.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2077..2424)
                     /locus_tag="JN755_002183"
                     /pseudo
     CDS             complement(2077..2424)
                     /locus_tag="JN755_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JN755_002184"
                     /pseudo
     CDS             complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JN755_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4431..5489
                     /gene="citC"
                     /locus_tag="JN755_002185"
     CDS             4431..5489
                     /gene="citC"
                     /locus_tag="JN755_002185"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7380970.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5504..5800
                     /gene="citD"
                     /locus_tag="JN755_002186"
     CDS             5504..5800
                     /gene="citD"
                     /locus_tag="JN755_002186"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7380971.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5797..6705
                     /gene="citE"
                     /locus_tag="JN755_002187"
     CDS             5797..6705
                     /gene="citE"
                     /locus_tag="JN755_002187"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7380972.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6716..8248
                     /gene="citF"
                     /locus_tag="JN755_002188"
     CDS             6716..8248
                     /gene="citF"
                     /locus_tag="JN755_002188"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY7380973.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8252..8803
                     /gene="citX"
                     /locus_tag="JN755_002189"
     CDS             8252..8803
                     /gene="citX"
                     /locus_tag="JN755_002189"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7380974.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8778..9656
                     /gene="citG"
                     /locus_tag="JN755_002190"
     CDS             8778..9656
                     /gene="citG"
                     /locus_tag="JN755_002190"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7380975.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9707..11170
                     /gene="citT"
                     /locus_tag="JN755_002191"
     CDS             9707..11170
                     /gene="citT"
                     /locus_tag="JN755_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7380976.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11284..12090
                     /gene="rna"
                     /locus_tag="JN755_002192"
     CDS             11284..12090
                     /gene="rna"
                     /locus_tag="JN755_002192"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7380977.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12319..12729
                     /gene="rnk"
                     /locus_tag="JN755_002193"
     CDS             12319..12729
                     /gene="rnk"
                     /locus_tag="JN755_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7380978.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12960..14197)
                     /locus_tag="JN755_002194"
                     /pseudo
     CDS             complement(12960..14197)
                     /locus_tag="JN755_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14418..14846
                     /gene="uspG"
                     /locus_tag="JN755_002195"
     CDS             14418..14846
                     /gene="uspG"
                     /locus_tag="JN755_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7380979.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="JN755_002196"
     CDS             complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="JN755_002196"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7380980.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="JN755_002197"
     CDS             complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="JN755_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7380981.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17712..18458
                     /gene="dsbG"
                     /locus_tag="JN755_002198"
     CDS             17712..18458
                     /gene="dsbG"
                     /locus_tag="JN755_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7380982.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18668..19570
                     /locus_tag="JN755_002199"
     CDS             18668..19570
                     /locus_tag="JN755_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7380983.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19717..19916
                     /locus_tag="JN755_002200"
                     /pseudo
     CDS             19717..19916
                     /locus_tag="JN755_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5016 a         5125 c         4758 g         5017 t
ORIGIN      
        1 ataacttgcg aaccgcaaag ccagttcccg ttattatcca tgtacgcctg accatcggtg
       61 ccattggctg tccttgagtt attaatcatg tagatgccaa attgcctatt ccccagccca
      121 ccaattacga atttacggtc ggtatggtcc tggcgaagca atgcctgcgc accatcgttg
      181 gataccgcat tacggcccat aataacgttc tggtcacgca tatgaatcca catgcctgta
      241 ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg taacaaccag accatgcccc
      301 atgcttatcc ggccagttct gagattaagc gtaaaggggc gtagtggccc tatattacca
      361 ttttcccctt cattctctcg tgtagggatg atatgcaggc attcttcaga acgacgaaaa
      421 atggcaccaa aagatgaatt aaatattctc agtgcattga ccgtcgatat ttttacttca
      481 ctgctgaaaa gggctttaac aagaacatac agagcatccc atttaagatt catcaggtct
      541 ttaacccggc cgccagtgag gtggtgtctc catttgaaat actcattgcc gttgtcgcct
      601 gtttcaaacc acatgtatga atcagtatcg ctgtcggcat catttttaaa tccaatcttt
      661 gcccagtcag tattccgaat ccaggcaagg attgagtcgt tttcaaaagt aagtccaccg
      721 gacaacgtat cgccattttt ttgcacggcg ttaccagcct tgtttaccgt ttcctgtaaa
      781 ccgaggggcc gtttctggcc tgcatggcat gatttccgct tttaaacggg gaattcagcg
      841 tgctgattgg ctatgtaagg gtatcaacaa atgaccagaa tacagacctg caacgaaacg
      901 ctcttgtttg tgcaggatgt gaacaaatat ttgaagataa attaagcgga acaaggacag
      961 accgaccggg attaaaacgt gctttaaagc gccttcaaaa aggaacgcat atagaaaacg
     1021 acgatcgaat caattaaatc gatctgtatt accgatcgat tatgtcccga taaccacacc
     1081 cgactgatga gattttttaa gataacggcg aattgatgca aggaggtgaa atgaaatcaa
     1141 ttcgctgtaa gaactgcaac aaactgttat ttaaagcgga ttcctttgat cacattgaaa
     1201 tcaggtgtcc gcgttgcaaa cgtcacatca taatgctgaa tgcctgcgag catcccacgg
     1261 agaaacattg tgggaaaaga gaagaaatca cgcattctga cgaaaccgtg cgttattgag
     1321 tatgaaggcc agattgttgg ctatggttca aaggagctgc gcgttgaaac catatcctgc
     1381 tggctggccc gcacaatcat tcagacaaag cactattccc gccgttttgt gaataactct
     1441 taccttcacc tgggagtatt cagcgggcgt gatctggttg gcgttctcca gtggggatat
     1501 gcccttaacc ccaactcagg tcgtcgtgtc gtgcttgaaa cggataaccg gggctatatg
     1561 gagttgaatc gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc
     1621 agctacgcgc tgaaagttat cagattactg tatccgtcag tggagtgggt tcagtccttt
     1681 gctgacgaac gctgcggact cgcaggcgtt gtgtatcagg cgtcgaattt tgattttatt
     1741 ggcagccatg aaagcacgtt ctacgagctg gatggtgagt ggtatcacga gataacgatg
     1801 aacgcgatta agcgcggagg gcaacgaggc gagtatttgc gagctaataa ggagcgtgcc
     1861 gtggtgcaca aatttaatca gtatcgctac atcagattcc tgaacaagcg agcaaggaag
     1921 cggctaaata ccaaactatt caaggttcag ccatacccta agtgatcccc atgtaatgaa
     1981 taaaaagcgg taattaatac tgctgtttca tttgaagcgc gaaagctaaa gttttcgcat
     2041 ttatcgtgaa acgctttcgc gtttttcgtg cgccgcttca cccagccgtt cataggcaat
     2101 tggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc
     2161 actggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag
     2221 taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa
     2281 acgctcgatc atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc
     2341 gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat
     2401 caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg
     2461 taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg
     2521 caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgacccctg
     2581 ctcaaatatt ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat
     2641 caccacatca tcgccttcat cgctgaggaa taattcaacg ctcttgtttc cttcatcgct
     2701 acgcaggctg gcttcgaagg cgttatcaag taaattgcca acaatggcgg caaactcggt
     2761 gctatccagt cctggcggca gttgcgaaag ctggctaccg gggacgatga tcatttttag
     2821 ccccagttcg cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc
     2881 aaacgcctcg cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat
     2941 cgccagcacg cgatcatact ctttcatctg caacagacca ttgagagtcg acatccaatt
     3001 gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg
     3061 cgcattgagg gtggaaattt cgtctttact gcgaaagctg ataatggctc ccagcaaatc
     3121 atcacctgaa cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac
     3181 cacatcctga cgtttttcat caatctgttc ggtaaagaaa tcggcagacc tgaccacttc
     3241 agcaatgggt ttacccaacc attgccgtcc aggggaactc agccccagca tctttcttgc
     3301 gttacgattg atggcggtaa tgtaaccgtg cggatccacc gcaatcagcc cttcataaac
     3361 cgaactaaac agcgcctctt gctgacggac cacccgtgcg atttgctttg gctccatgcc
     3421 catcatctgc cgacggatat gcgcggccag gaaccacgac agcaacatca gaatccctaa
     3481 cagcacgaca aacacacctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt
     3541 actcaccagg tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg
     3601 cgttttggcg cgcatcgcca tacccattga ccctttcccg gtaatgaagt agctctcccc
     3661 tttctccagc gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg
     3721 atggtaaagg cggatcgagt gccggtcgcc aatcaccaca taatcaaaat cggtatctct
     3781 ttgtaatttg ttagcgatgg tcgccagccg tttgtagtca cgcgttttca ccgcagagat
     3841 gacactgtca ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag
     3901 cgttaaatag tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat
     3961 tgagaacacc agaatcagca aaaagatacg cagcggaaat gtcagtcttt ggaaaaatgc
     4021 aaactgttta ttctcgttaa gctgcaacat tgttttcctt gcgggttgtc ccgagcctct
     4081 ttgacggatg taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca
     4141 aattttattc attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt
     4201 aattaaaaaa cttaaaacca ttaaacaaat aaaaaaacca ctaactctat gtgaaataaa
     4261 tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatacc
     4321 tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca acattgaaca
     4381 acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt atgttcgtca
     4441 atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat
     4501 tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc
     4561 gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa tgcgttgcta
     4621 tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa ctgataaacc
     4681 tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa tacgaggcgc
     4741 tgttccgcca gtgcggtttt tccacgctga cctgcgtacc cggcgtgatg gtgctgatgg
     4801 aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct gaaaaaattg cgtcatccag
     4861 ggaacaagat tggctgcatt gtgatgaacg ccaatccctt tacgaatggt caccgttatc
     4921 tgattcaaca agctgcggca cagtgcgact ggtttcatct gtttttagtc aaagaagatt
     4981 cttcacgctt cccctatgaa gaccggctgg atctggtgtt aaaaggcacc gccgatattc
     5041 cacgcctgac tgtgcatcgc ggctcagaat acatcatctc ccgcgctacg ttcccttgct
     5101 acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat ctgaagattt
     5161 tccgtcagta cctcgctccc gcgcttggtg taactcaccg ctttgtcggt actgaaccct
     5221 tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa acgccgacta
     5281 tctccgcagc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag gagatgccga
     5341 tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct atcgcgccgc
     5401 tggtccctgc agtcacgctg cattatttgc agaacctgct tgaacactcc cgccaggacg
     5461 cggcagctcg tcaaaagacc cccgcatgag aaacaggtga aaaatgaaaa taaaccagcc
     5521 cgccgttgca ggcacccttg agtctgggga tgtgatgata cgcatcgccc cactcgatac
     5581 gcaggatatc gacctgcaaa tcaatagcag cgttgagaaa cagtttggcg atgcaattcg
     5641 caccaccatt ctggacgttc tcgcccgcta caacgtgcgc ggcgtacagc tgaatgtcga
     5701 tgacaaaggc gcactggact gcattttacg tgcacgactg gaagccctgc tggcacgcgc
     5761 cagcggtatc ccggctctgc catgggagga ttgccaatga tttccgcttc gctgcaacaa
     5821 cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg gtgccaatgc cgcgatggtc
     5881 agcaactcct tcatctaccc ggctgatgcc ctgatgtttg acctcgaaga ctccgtggca
     5941 ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg cgctgcaaca tccgctgtat
     6001 cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt ccgaatgggg tgttaacgac
     6061 ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc tgccgaaaac cgataccgct
     6121 caggatgttc tggatattga aaaagagatc ctgcgtatag aaaaagcctg tgttcgtgaa
     6181 cccggcagca ccggcctgct ggcggcgatt gaatctccgc tgggcattac ccgcgcagtg
     6241 gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc tcggtgcaga agactatgtg
     6301 cgcaacctgc gtacagaacg ctccccggaa ggaactgaac tgctgttcgc acgctgttcc
     6361 attttgcagg ccgcgcgctc tgcgggtatt caggcgttcg ataccgtcta ttccgacgct
     6421 aacaacgaag ccggattcct gcaagaagcc gcccacatca aacagctggg ctttgacggc
     6481 aaatcgctga tcaacccgcg tcagattgat ctgctgcaca acctctacgc accgacccag
     6541 aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg aagccgccgc tcgcgaaggc
     6601 ctcggcgtgg tttccctgaa cggtaagatg gtggacggtc cggttatcga tcgcgcccgt
     6661 ctggtgctct cccgtgcaga actttccggc atccgcgaag aataaggcaa tcaaaatgac
     6721 gcagaaaatt gaacaatctc aacgacaaga acgggtagcg gcctggaatc gtcgcactga
     6781 atgcgatctt gccgctttcc agaactcacc aaagcaaacc taccaggctg aaaaagcgcg
     6841 cgatcgcaaa ctgtgcgcca acctggaaga agcgattcgt cgctcaggtt tacaggacgg
     6901 catgacggtt tccttccatc acgctttccg tggcggtgac ctgaccgtca atatggtgat
     6961 ggacgtcatc gcgaagatgg gctttaaaaa cctgaccctg gcgtccagct ccctgagtga
     7021 ttgccatgcg ccgctggtag aacacattcg ccagggcgtg gttacccgca tttatacctc
     7081 tggcctacgc ggtccactgg cggaagagat ctcccgtggt ctgctggcgg aaccggtgca
     7141 gatccactct cacggcggtc gtgtgcatct ggtacagagc ggcgaactga atatcgacgt
     7201 ggctttcctc ggcgtcccgt cctgtgatga attcggtaat gccaacggct acaccggtaa
     7261 agcctgctgc ggctccctcg gctatgcaat ggttgatgcc gacaacgcaa aacaggtcgt
     7321 gatgcttacc gaagaactgc tgccttatcc gcataatccg gcaagcattg agcaagatca
     7381 ggttgatttg atcgtcaaag ttgatcgcgt tggcgatgct gcaaaaatcg gcgctggtac
     7441 tacccgtatg accactaacc cgcgcgaact gcttattgcc cgtagcgctg cggatgtgat
     7501 tgtcaactct ggctacttca aagaaggttt ctccatgcaa accggcaccg gcggcgcttc
     7561 gctggcggta acccgtttcc tggaagacaa aatgcgtagc cgcgatattc gcgccgactt
     7621 cgccctcggc ggtattaccg cgacgatggt tgatctgcac gaaaaaggtc tgatccgcaa
     7681 actgctggat gtgcagagct ttgacagcca tgctgcgcaa tcgctggccc gtaaccccaa
     7741 tcacatcgaa atcagcgcca accagtacgc taactggggt tcgaaaggcg catcggttga
     7801 tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac aggcagttca acgttaacgt
     7861 gctgaccggt tctgacggcg tactgcgtgg cgcttccggt ggtcactgcg ataccgcgat
     7921 tgcctctgcg ctttccatca tcgtcgcgcc actggtacgc ggtcgtattc cgactctggt
     7981 ggataacgtg ctgacctgca tcaccccggg ctccagtgtc gatattctgg tcacagacca
     8041 cggtatcgca gttaacccgg cacgtccgga actagcagaa cgtctgcagg aagcgggcat
     8101 taaagtggtt tccattgagt ggctgcgcga acgtgcgcgt ctgctgaccg gtgaaccaca
     8161 gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt taccgcgatg gctcggtgat
     8221 cgatgttgtg catcaggtga aggaataagc catgcacctg cttcctgaac tcgccagcca
     8281 ccatgcggtg tcaattcccg agctgctcgt cagccgggat gaaaggcaag cacggcaaca
     8341 cgcctggctc aagcaccatc ctgttccact ggtctccttt accgtggttg cgcctgggcc
     8401 gattaaagac agcgaggtca cacgccgaat ttttaatcat ggcgtgacag ccttgcgtgc
     8461 cttagccaca aagcagggct ggcaaattca ggagcaggct gcactggttt ccgccagcgg
     8521 gccggagggc atgttgagca ttgccgcccc ggctcgcgat ctcaagctcg ccaccattga
     8581 gcttgaacat agtcatcctc tcgggcggtt atgggatatc gatgtcctga cgcccgaagg
     8641 cgaaattctc tcccgccgcg actattcact gccgcctcgc cgctgcctgt tgtgcgagca
     8701 aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac tcaaccgcat
     8761 ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca aagcttgcga
     8821 cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact gaagtcaatc
     8881 tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa gatatggcgc
     8941 tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt ttcattgaat
     9001 ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta cgcccaattg
     9061 gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg cataaaggca
     9121 gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag ctcaaccagc
     9181 cggttacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggc ctgaccgatc
     9241 gcgaactgcg taccaataat tcacgactga cggcaggtca acggttgtac cagcagcttg
     9301 gccttaccgg cgcacgcggt gaagccgaag cgggttatcc actggtgatc aaccacgcct
     9361 tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca ttgctcgata
     9421 ccttgctcct actgatggct accaacggcg ataccaacgt tgcatcgcgc ggtggcgagg
     9481 ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg ggcattcgaa
     9541 cccccgccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa cgaaatctca
     9601 gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt tttagcacag atttaattat
     9661 ttaagcactt gataaatttg gaaatattaa ttttcggaga acccgtatgt ctttagcaaa
     9721 agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt ttcttatccc
     9781 tgtccccgac ggtatgccgc cgcaggcatg gcattacttc gctgtgtttg tggcaatgat
     9841 tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg cggttactat
     9901 ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg acccagcgtt
     9961 taatgcgcaa aaacaggcgc tgaaatgggg cctggctggt ttttccagca ccacggtatg
    10021 gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt taggtcgtcg
    10081 cattgccctt ttcctggtga aattcatgga caaacgcacg ctgacgttgg gttatgcgat
    10141 tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc gtaccggggg
    10201 tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc cgaacgatcc
    10261 gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta ccagtctgag
    10321 ttcatccatg tttgtcaccg gtgcggcacc aaacatgctg ggtctggagt tcgtcagcaa
    10381 aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggggt
    10441 tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca
    10501 cagtgaagaa gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg
    10561 cagagagtgg acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag
    10621 tgaagtcatt aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca
    10681 cgttgtgccg tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct
    10741 ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc
    10801 cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt
    10861 tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat
    10921 gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg
    10981 tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc
    11041 tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc
    11101 aatcttcggg gtgatttaca tctctatgtt actgttggtt ggctggccga ttctcgccat
    11161 gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccgccag gtaagcccac
    11221 ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc
    11281 attatgaaag cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct
    11341 tctgccaacg ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
    11401 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
    11461 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
    11521 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
    11581 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg
    11641 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
    11701 ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc
    11761 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
    11821 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
    11881 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
    11941 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
    12001 aacgctccgc tttctgcaaa ctcattcttg ccacaacctc acccaggtaa ctgtggcaaa
    12061 acctttgtga ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt
    12121 cttccataac catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga
    12181 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
    12241 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
    12301 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
    12361 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
    12421 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
    12481 acaatgaaca gccgggttaa attccgcaat cttagagatg gcgaagtgcg tgtgcgcacg
    12541 ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta
    12601 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
    12661 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
    12721 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
    12781 ctctttacgc aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt
    12841 attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc
    12901 tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat
    12961 catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct
    13021 gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa
    13081 atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt
    13141 ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc
    13201 tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata
    13261 aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac
    13321 tggcgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg
    13381 gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct
    13441 gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc
    13501 gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc
    13561 tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca
    13621 tagactgcaa cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt
    13681 ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac
    13741 ggccccacat tccctttagg gacgcggaca tattccgcct gcccgctagg aacgccacca
    13801 tacaggtgac tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca
    13861 gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa
    13921 cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt
    13981 acgtccttcc cggtttcaac gacttcccca taaattcatg accaaaaata tcgccatgtt
    14041 taacctgggg tattttgcct cgataaagat ggaggtcaga gccacagatc gccgttaccg
    14101 taatacgcag aataatatca tctgcctgtt caatgcccgg atcgggaaca ttttctacct
    14161 gaacgtgatg tgggccgtga taagtcaatg ctttcatgca acctccagag acagttcaaa
    14221 aaagagtcag tctttaaggg tagctatcac agcgggaaaa cgggcggtac cgcaggtttt
    14281 gggagaattc ctggtgttca gtgagaatat attaatcaca gaaaccgttc gtaaggaaaa
    14341 agaaccagac aaatatcagg gataaatatt ataatcatgg cgaatgattt gttcatgatt
    14401 aacagggaga aagggttatg tataaggcaa tcattatgcc agttgatgta tttgaaatgg
    14461 aattgagcga caaagctgtt cgccacgctg aattcctcgc ccaggatgac ggagttattc
    14521 atctacttca cgtactaccc gggtcagcca gcctgagcct gcaccgtttt gccgctgatg
    14581 tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga acgtctgcaa acgatggtca
    14641 gccacttcac catcgatcct tcccgcatta aacaacatgt ccgttttggt agcgtgcggg
    14701 atgaagtcaa tgagttggca gaagaactgg gggctgatgt tgtagttatc ggttctcgca
    14761 acccatcgat ttcgacccat ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca
    14821 atctgcctgt actggtcgtt cgctaacaga taaaagtatt tatgccgccc tgcctggaac
    14881 atcccggcag ggctttttaa tgccataaaa aagccgccag gtttgacccc ggcggctaag
    14941 caattgcagg tgaatcttac ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa
    15001 gcactcagag aggctttggc accttcgcca gtggcgatga tgatctgctt gtacggaacc
    15061 gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg tttcacattt cgcatcaatg
    15121 ataatctcgc ccatgcggtt acgttcgact gcgccttcca gccagttggt gttcgggagc
    15181 agaccaatct ggacaaaaat accggccagt tcgatgttgt gaatattgcc gctgacacga
    15241 tctcgatatt ccagaccaac gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca
    15301 ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt cctgcagaac ctggtcggct
    15361 ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa cgatacccgc caggtcgatt
    15421 gccgcttcca cgccggagtt accgccgccg ataaccgcta cgcgtttgcc tttaaacagc
    15481 gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc gatactgatc ttcgcccgga
    15541 acgttcatgt tgcgccattt tgcacccgtc gccacgataa tgctgcgtgc tttcagtacc
    15601 gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa ccgctgccgg gatcagtttg
    15661 ctggcgctct ggctgtcgat cacatcaacg tcgtattcat caacgtgaac tttcagtgcg
    15721 cctgccaact tctgcccttc agtcttcggt acagaaatgt agttttcgat atcaacggta
    15781 tcgaggatct gaccaccaaa acgttcgccc atcagaccgg tacggatgcc tttacgtgcg
    15841 gagtaaattg ctgccgctgc acccgccggg ccggaaccaa cgattaatac gtcataagca
    15901 tcacgcttgt tcagctcttc tgccgcacgt ttttccgcgc cagtatcaat tttggcaacg
    15961 atttcagtca acgtcatgcg gccctgacca aactctttcc cgtttacgaa cactgccgga
    16021 acgcccatca cgttgcgatc ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg
    16081 tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg cctgcaccac gtccgggcag
    16141 ttatggcaag agagcgagta ataggtttcg aattcaaaat caccgtcaat atggcgaatc
    16201 tgctccagca gagactgcgc ttctttcgac ggatgaccac cggtccacag caacgccagt
    16261 accagcgagg tgaactcgtg gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa
    16321 cctgggttgg tgatcaggaa agacggctta cgcactggca agctgttatc ttctttaaag
    16381 gtgactttgt ctgacagttc tgcgatttca gccaacagtt ccttgatttc tgccgattta
    16441 gcgctgtcat ccagcgtggc aattaactca acaggcttgg tcaatttctc aaggtaagcc
    16501 ttgagttgag ttttcatatt tgtgtcgagc atgtttatct cctgggcttt aaacatcatc
    16561 atgcaagcgg cctctgcggc tacctggatg cagcttgcat catggtgcgg ggaagaaaat
    16621 cgggtgccgc agcacccgaa gaattaggtg aatttccgaa gtatttcgcg tcagagcaag
    16681 gcgactagcc tgtgaatccc cgggagctta cacaagtaag tgaccggggt gagcgggcga
    16741 cgccaacgcc gctatggcgt gaaagacgaa ggaaatttag attttaccaa ccaggtccag
    16801 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    16861 agaagctacg tactgagctg ctttgatttt acgcagcaga tcagacgcgt cacggccaat
    16921 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    16981 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    17041 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    17101 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    17161 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    17221 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    17281 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    17341 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta
    17401 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    17461 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    17521 aatctgaact accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac
    17581 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    17641 agctcttacc tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa
    17701 agaacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    17761 cagaggaact tccttcccca gtaaaagcga ttgaaaaaca tggcattaca atcatcaaaa
    17821 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    17881 ccatctacct gactcccgat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    17941 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    18001 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca
    18061 ttgtctacgt cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    18121 gcccgtgggt agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc
    18181 cagaaagccc ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    18241 aacaatatga agcctctggt tgcaagctta agctaaacgt gcctgcaaac gtaagtacag
    18301 agcaaatgaa agtgttaagt gacaatgaga aactgatgga cgatctgggg gcaaatgtca
    18361 cgccggctat ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg
    18421 atcagaaaac gcttaatatc attatgggaa ataaataaaa ggtaattcat ggatggttga
    18481 actatatccc tgcttttcaa ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt
    18541 ggctgtgcta ttttagaaaa acttaatcct gagttaagca aaaaaacata atcaataaaa
    18601 atacatacgc aaaaaacatt gattaagtga atataccatg gaagaaaaat ataaaccgga
    18661 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt
    18721 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
    18781 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
    18841 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
    18901 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
    18961 aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
    19021 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
    19081 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
    19141 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
    19201 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
    19261 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
    19321 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaataa ttaatagcat
    19381 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactacc accgtgactt
    19441 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
    19501 ctataataaa tcttctttaa ataatttggt gttctcccga tttattgacc gtctaaatga
    19561 gagtttttga tatataacta cagacagcta ctataacttt catctattta ttcacagctc
    19621 gaatgaaaaa taaaacactc tgctttacag tgtttacttt ataattaaca gtcttatctc
    19681 actgttcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
    19741 ccactcaata ttctggaagc tgcacgagag cgtattacct agacactcaa caccctaccc
    19801 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
    19861 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggc
//
LOCUS       DADLTI010000069        19787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLTI010000069 DADLTI010000000
VERSION     DADLTI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.069"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            10..276
                     /locus_tag="JN755_002201"
     CDS             10..276
                     /locus_tag="JN755_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7380984.1"
                     /translation="MNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVV
                     TLIDDNIREEILRPEWIAGETGMSVRSLYRMFADIRFGSRAIYS"
     gene            251..442
                     /locus_tag="JN755_002202"
     CDS             251..442
                     /locus_tag="JN755_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7380985.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(614..943)
                     /locus_tag="JN755_002203"
     CDS             complement(614..943)
                     /locus_tag="JN755_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY7380986.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(948..1133)
                     /locus_tag="JN755_002204"
     CDS             complement(948..1133)
                     /locus_tag="JN755_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7380987.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1130..3769)
                     /locus_tag="JN755_002205"
     CDS             complement(1130..3769)
                     /locus_tag="JN755_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY7380988.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3977..4966
                     /gene="ldhA"
                     /locus_tag="JN755_002206"
     CDS             3977..4966
                     /gene="ldhA"
                     /locus_tag="JN755_002206"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7380989.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5077..5424
                     /gene="hslJ"
                     /locus_tag="JN755_002207"
     CDS             5077..5424
                     /gene="hslJ"
                     /locus_tag="JN755_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7380990.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5421..5687)
                     /locus_tag="JN755_002208"
     CDS             complement(5421..5687)
                     /locus_tag="JN755_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7380991.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5961..9485
                     /gene="nifJ"
                     /locus_tag="JN755_002209"
     CDS             5961..9485
                     /gene="nifJ"
                     /locus_tag="JN755_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY7380992.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9852..10981
                     /gene="ompN"
                     /locus_tag="JN755_002210"
                     /pseudo
     CDS             9852..10981
                     /gene="ompN"
                     /locus_tag="JN755_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11122..11556
                     /gene="uspF"
                     /locus_tag="JN755_002211"
     CDS             11122..11556
                     /gene="uspF"
                     /locus_tag="JN755_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7380993.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11732..12667
                     /gene="ttcA"
                     /locus_tag="JN755_002212"
     CDS             11732..12667
                     /gene="ttcA"
                     /locus_tag="JN755_002212"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7380994.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JN755_002213"
     CDS             complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JN755_002213"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7380995.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JN755_002214"
     CDS             complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JN755_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7380996.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15879..17111
                     /gene="dgcM"
                     /locus_tag="JN755_002215"
                     /pseudo
     CDS             15879..17111
                     /gene="dgcM"
                     /locus_tag="JN755_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JN755_002216"
     CDS             complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JN755_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7380997.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17949..19403)
                     /locus_tag="JN755_002217"
     CDS             complement(17949..19403)
                     /locus_tag="JN755_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7380998.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19400..>19787)
                     /locus_tag="JN755_002218"
     CDS             complement(19400..>19787)
                     /locus_tag="JN755_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7380999.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5203 a         5011 c         4665 g         4908 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gatataaggt ttggtagtcg cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga
      301 tgcgattcgc catgccgcag atgatgaaaa actggcaggc atcggctttc attggggatt
      361 ttctgaccag agtcatttct caacggtatt taagcaacgc tttgggatga cgccaggcga
      421 gtatcgccgt aaattccgct aactaattgg cgttgcatgt actgcaacgc caattagtta
      481 aaacagatat ttgactatgc atttttcagg gcgaaaggca atttgacagg agtttttgaa
      541 tattcacctt gtttcaggaa aatagatcaa tcgctttctc ctgtagcgtt atcccgatat
      601 tgcggttttg ctattaaaac tttcgcaccc atttaccgtt tatcccttgt acatattccc
      661 ccggtttggc ccgagctacg agcttttgcc cggcgagttt cgcgatatca tctacagaca
      721 cattattttg ctttgcccgt tgttggtaac tcgctttacg ttcggcatta atttcggtta
      781 ctaatttctc tgtttcagca tccgttttca gcgcaaccag ataaccataa aatgtttcac
      841 ctacccgccc ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca
      901 gcccaacaag aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg
      961 ctacgagttt caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca
     1021 attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc aatgcgaggc
     1081 gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt
     1141 cctcacactc ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc
     1201 atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat
     1261 gatggtaatt aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt
     1321 tggcctgcat ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa
     1381 tttccatata gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg
     1441 catctgcggt atctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat
     1501 ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg
     1561 acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca
     1621 gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc
     1681 ctaacacatc gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac
     1741 gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc
     1801 gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca
     1861 ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca
     1921 cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat
     1981 acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag
     2041 tcagtgattg ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat
     2101 ttacccgaac cgggccaatc tcaccaacat gtaaatcgcc tttaaagagg aaataggtcg
     2161 gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga
     2221 acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac
     2281 gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt
     2341 attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact
     2401 caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt
     2461 agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca
     2521 gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa
     2581 caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg
     2641 aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc agcgttgggt
     2701 cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt
     2761 tcgcttcacc ggtcagctgc agaggcagtt gactgttatc catgcttaat tttcctgggc
     2821 caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca ctcagtcgtc
     2881 cgctgaccag agcgttctca agccctgcct gccagttgtc aactttgaca cccagtcggc
     2941 cactcaaagg aaaacctgca tacagccagc tccagcgacc atcgcttacg gtcaattgtt
     3001 gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga
     3061 tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac ggttcctgcg
     3121 gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca
     3181 tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg acatccagtt
     3241 cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat
     3301 aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc
     3361 agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact
     3421 gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag
     3481 cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg
     3541 cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta
     3601 aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc
     3661 agatgtccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa
     3721 gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat
     3781 atcctgattt cgcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc
     3841 agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatgg
     3901 ctttcaatta tatttgaaat tttgtaaaat atttttagca gcttaaatgt gattcaacat
     3961 cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta
     4021 cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac
     4081 ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga
     4141 cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg
     4201 ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt
     4261 ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac
     4321 gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga
     4381 aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat
     4441 cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc
     4501 gtatccaagt gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt
     4561 ctctgaatca gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt
     4621 gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg
     4681 tgcattgatt gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt
     4741 gggtatggac gtctatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt
     4801 gatccaggat gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca
     4861 ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt
     4921 aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gccgctcccc
     4981 tgcaatccag gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa
     5041 atagtcctga gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt
     5101 gcgctaagcc tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa
     5161 atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     5221 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     5281 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     5341 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     5401 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     5461 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg
     5521 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     5581 gcttttaaac ttggcgctac gtgcgccgca gtcgcctgct gaacaggctc actactacac
     5641 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct
     5701 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     5761 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5821 tgatcaaaac attcattact ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5881 catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5941 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     6001 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     6061 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     6121 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     6181 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt
     6241 acaaactggc aggcgaactg acgccatttg tcctgcatgt agcggcacgt accgttgcca
     6301 cacatgcact ttctattttt ggcgatcatt ccgacgttat ggcggtgcgc cagacgggtt
     6361 gcgcgatgtt gtgtgcggct aacgtccagg aagcgcaaga ctttgctctc atttcacaca
     6421 tcgcaacgct gaagagccgc gtgccattta ttcatttctt tgatggtttt cgcacgtccc
     6481 acgaaatcaa taaaattgtc ccgctggccg atgacactat tcttgatctc atgccgcagg
     6541 ccgaaattga tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgcggta
     6601 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaacccc tggtacaacg
     6661 cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca ggtcgtcagt
     6721 atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     6781 ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagttg
     6841 gcgtgctgaa agttcgtctg taccgtccct tctccgccaa acacttgctg caagctctgc
     6901 cgggatccgt acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     6961 aaccgctcta tctggatgtg atgaccgcac tggcaggagc ctttaataat ggtgagcgcg
     7021 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     7081 actgtgtact ggcggtattt gccgagctca acgcggctaa acctaaagca cgctttacgg
     7141 ttggtattta cgatgatgtg accaatctgt cactgccgtt gccggaaaac actctgccaa
     7201 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcaa
     7261 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     7321 tttacgactc caaaaaagcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     7381 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     7441 ttatcgataa atatcagatg gctgagcgtt taaaacctag cggcattttc ctgcttaata
     7501 cgccgtacag cgcagatgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgttaa
     7561 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     7621 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     7681 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     7741 gtcaggatct ggtggagcgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7801 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     7861 ccgcccctga tttcgtgaaa accgtaaccg cggcgatgct cgccgggctt ggtgatgccc
     7921 tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccact cgctgggaaa
     7981 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     8041 actgcgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg cctgaagcga
     8101 tggaaaacgc ccctgccagc ctgcattcgc tagatgtgaa atcgcgtgat atgcgcgggc
     8161 agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     8221 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc
     8281 aggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     8341 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     8401 aatattcagg tgcttgcccc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     8461 tctatggtga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     8521 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     8581 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     8641 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     8701 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     8761 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     8821 aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc
     8881 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8941 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaagttcg
     9001 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     9061 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     9121 aggaagcgga agcgtatccg ggaccatcgc tgatcattgc ttatagcccg tgtgaagagc
     9181 atggttacga tctggctctc agccacgacc agatgcgcca actcactgct accggcttct
     9241 ggccgctata tcgctttgat ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     9301 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcag cgtttccgtc
     9361 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     9421 tgcaaaaacg ctatgacttc ctagcacaaa tggccggaaa agcggaaaaa agcaacaccg
     9481 attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg
     9541 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     9601 tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa
     9661 tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca tttcagagga
     9721 attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc aaactgaact
     9781 tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa aataactaaa
     9841 ggatttattc aatgaaaaga aaagtactgg cacttttaat ccctgccctg ctcgccgcag
     9901 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
     9961 aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg
    10021 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca acttactggc tacggtcaat
    10081 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
    10141 tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
    10201 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc taaatttggc ggtgactctt
    10261 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
    10321 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
    10381 aaggagccag taatggtcag gaaggcacca ataacgaacg cgatgttcgt catgaaaacg
    10441 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
    10501 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
    10561 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctagcaacca
    10621 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgca gttgcgaaca
    10681 aaacccagaa ctttgaagtt actagtacca gtttgatttt ggtctgcgtc cggcagtctc
    10741 tttcctgatg tctaaaggcc gtgaccttca cgctgctggt ggtgcagaca acccggcagg
    10801 tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact atttcaataa
    10861 aaacatgtcc acctatgttg actataaaat caacctgttg gatgaagatg acagtttcta
    10921 cgctgccaac ggcatctcta ccgatgatat tgtcgcttta ggtctggttt atcagttcta
    10981 aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc tttgatctaa
    11041 attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac actttatgcg
    11101 gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga tatttcagat
    11161 tcagaattaa ctcaacgcgt gattagccac gttgaggcag aggcaaagat tgatgatgca
    11221 gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct gggtttagcg
    11281 tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc gcaactggaa
    11341 gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt tgaggaaggc
    11401 tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat gatcatcatt
    11461 gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc agctgtagtg
    11521 cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca cattgctgcg
    11581 ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg taccatacac
    11641 gccacagttt ttatatcaga tttcttatgc tgagcgttcc ggcatgacgc atactcttct
    11701 gatgccatat aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagtaagaa
    11761 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
    11821 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
    11881 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
    11941 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
    12001 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
    12061 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc ggctacgtcg
    12121 cggtatcctt tatcgcaccg caacggaact ggggacgacg aagatcgcgt tgggtcacca
    12181 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
    12241 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cactggccta
    12301 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
    12361 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
    12421 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
    12481 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcattaccc acggttctga
    12541 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
    12601 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
    12661 gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga
    12721 taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg
    12781 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
    12841 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
    12901 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
    12961 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
    13021 tgccatttct gcttccagcg tcacaatgga actattagct ggcggcgttt gccagttaag
    13081 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
    13141 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
    13201 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
    13261 acgcgcagca acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag
    13321 ggtctgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgct ctacttcatt
    13381 cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga
    13441 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc
    13501 ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg
    13561 atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
    13621 aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
    13681 catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac
    13741 cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc
    13801 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
    13861 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
    13921 acgcgtagga cacagcacta aagccagggt ttgaaatagc gacgcatcaa tttgctgtaa
    13981 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
    14041 ttttcctgca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
    14101 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt
    14161 cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta
    14221 cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc
    14281 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
    14341 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
    14401 gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
    14461 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
    14521 atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta
    14581 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccgaaaaa attgcttttg
    14641 ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag
    14701 aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac
    14761 gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
    14821 ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacgcc
    14881 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
    14941 ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
    15001 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
    15061 agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
    15121 tttatcgtgc agctgctcga taaattcact ggaatgatcg gtcaacgcat cgcacacatc
    15181 caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc
    15241 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
    15301 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt
    15361 aatcagcgtg ccttcaccga aacggctgac tcggggccgt gtgctctcgc ccgccagcgc
    15421 atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg
    15481 tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag
    15541 cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac
    15601 gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta
    15661 gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa
    15721 ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg
    15781 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
    15841 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
    15901 ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt
    15961 atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
    16021 gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
    16081 aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct
    16141 gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg
    16201 aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
    16261 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccgt
    16321 cacgagatgg acaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc
    16381 atgaaacgat gtgccagaaa catacctagg aaataaatat gcttgggcgt cgcgtcatgc
    16441 ctatcatgca tgaaatttcg catttacccg gcggtcataa ccctttgaat tttgttcata
    16501 aactggcgga tggttctact cgtcatgtgc agacctatgc cggaccgatt gaaatttatg
    16561 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
    16621 agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt
    16681 atcacattac ggaaccaggc caaatgcagc atctcgccat cgctcaggat tacagcttgt
    16741 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
    16801 atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg cgctcgcaaa ggcgatttgg
    16861 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
    16921 cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa agtgagtatt tcgggcttac
    16981 cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac
    17041 tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc
    17101 tggcggcata agccgcggat gcgtctcgag atcaacgact gcgcttagcg tggcgctccc
    17161 agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc
    17221 catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
    17281 ccacatagct gcggacaata ttggcatgtg atttatcttc ccgcccttta ccatgaataa
    17341 tcagcacatt acgcaaacca tccgccaggg cttgttgaat gaaactgaac accattttgc
    17401 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
    17461 tgcgcagctt atccagcacc ccatgctgca acccttcctg ccgaaactcc agcggctgac
    17521 ttagcgggat gatgtcgaga aatccggtag tgaggaaatt atcaagctgc agcgtgtcga
    17581 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatctgta gcacgtttca
    17641 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgtaac
    17701 ctcctatgca ccttcacacg tcgtgattca gacgggttaa ttgttcatac ttactgcatc
    17761 gttcccttca cagattgaaa caatactcta cttcatacct gtgagtatct gcgcaggcgg
    17821 ggcattttat actagcctgt tagcagtatc gaacagttta gcccgagttt aattcagcac
    17881 aggctccgat caggatgatt tattcatagc aggaaccaac aaaaatgcga taaataacag
    17941 accactgatt agcagaaagg taccgaaaat gccatagttt gcgcctccct cagcatcaat
    18001 tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttct actgcgatta
    18061 aaaaataaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt
    18121 tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca
    18181 ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc
    18241 cgttcttttg gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc
    18301 aaaatccctc taattgcctg tggaaacagt aattcctgcc atccccattc gtgggttata
    18361 ggggtaaaaa gatacattga aaacacaaat ccgcccagcc ccgccatcaa cagccatcgc
    18421 agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct
    18481 gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaatctg ccccaaaaac
    18541 acaggtatta aatatacagt agagaatatc ccaacccctc cagaaaaaga aaaaaaacat
    18601 cccagagtga aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta
    18661 agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata
    18721 gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc
    18781 agagtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagagatca
    18841 ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc aggtaatata
    18901 ttgatataaa atagccatcg ccagtcgagg ttatcagtga gccagccccc cagagttggc
    18961 cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa ccccaactct
    19021 ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagca
    19081 gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag tccacacgcg
    19141 atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc
    19201 cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata gctggtctga
    19261 agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac
    19321 gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca
    19381 atatgtttac gcatagacat catctctcat caacactgac ggtgacggac agtcccgggc
    19441 gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa
    19501 caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg
    19561 tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac
    19621 ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat
    19681 caacccacaa ctcactgact ggtactagtg aaactaacga tgtgccgcct tccacccaac
    19741 tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgca
//
LOCUS       DADLTI010000070        19439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLTI010000070 DADLTI010000000
VERSION     DADLTI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.070"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(32..1273)
                     /locus_tag="JN755_002219"
     CDS             complement(32..1273)
                     /locus_tag="JN755_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7381000.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(1273..1455)
                     /locus_tag="JN755_002220"
     CDS             complement(1273..1455)
                     /locus_tag="JN755_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381001.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(1467..2963)
                     /locus_tag="JN755_002221"
                     /pseudo
     CDS             complement(1467..2963)
                     /locus_tag="JN755_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3197..3691)
                     /locus_tag="JN755_002222"
     CDS             complement(3197..3691)
                     /locus_tag="JN755_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7381002.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3817..4167)
                     /locus_tag="JN755_002223"
     CDS             complement(3817..4167)
                     /locus_tag="JN755_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7381003.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            complement(4218..4550)
                     /locus_tag="JN755_002224"
     CDS             complement(4218..4550)
                     /locus_tag="JN755_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381004.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            complement(5013..5405)
                     /locus_tag="JN755_002225"
     CDS             complement(5013..5405)
                     /locus_tag="JN755_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAY7381005.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            complement(5389..5865)
                     /locus_tag="JN755_002226"
     CDS             complement(5389..5865)
                     /locus_tag="JN755_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY7381006.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            complement(5869..6195)
                     /locus_tag="JN755_002227"
     CDS             complement(5869..6195)
                     /locus_tag="JN755_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY7381007.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            6492..7823
                     /locus_tag="JN755_002228"
     CDS             6492..7823
                     /locus_tag="JN755_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HAY7381008.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTELKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            complement(7852..8220)
                     /locus_tag="JN755_002229"
     CDS             complement(7852..8220)
                     /locus_tag="JN755_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY7381009.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(8235..9224)
                     /locus_tag="JN755_002230"
     CDS             complement(8235..9224)
                     /locus_tag="JN755_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7381010.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(9232..10029)
                     /locus_tag="JN755_002231"
     CDS             complement(9232..10029)
                     /locus_tag="JN755_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7381011.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(10049..10438)
                     /locus_tag="JN755_002232"
     CDS             complement(10049..10438)
                     /locus_tag="JN755_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7381012.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            complement(10435..10761)
                     /locus_tag="JN755_002233"
     CDS             complement(10435..10761)
                     /locus_tag="JN755_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7381013.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(10758..11411)
                     /locus_tag="JN755_002234"
     CDS             complement(10758..11411)
                     /locus_tag="JN755_002234"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY7381014.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(11411..11905)
                     /locus_tag="JN755_002235"
     CDS             complement(11411..11905)
                     /locus_tag="JN755_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7381015.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11902..12843)
                     /locus_tag="JN755_002236"
     CDS             complement(11902..12843)
                     /locus_tag="JN755_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7381016.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(12833..13012)
                     /locus_tag="JN755_002237"
     CDS             complement(12833..13012)
                     /locus_tag="JN755_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7381017.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(13188..13739)
                     /locus_tag="JN755_002238"
     CDS             complement(13188..13739)
                     /locus_tag="JN755_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381018.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(13783..13983)
                     /locus_tag="JN755_002239"
     CDS             complement(13783..13983)
                     /locus_tag="JN755_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7381019.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            14074..14748
                     /locus_tag="JN755_002240"
     CDS             14074..14748
                     /locus_tag="JN755_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY7381020.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            14921..15217
                     /locus_tag="JN755_002241"
     CDS             14921..15217
                     /locus_tag="JN755_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381021.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            15493..15803
                     /locus_tag="JN755_002242"
                     /pseudo
     CDS             15493..15803
                     /locus_tag="JN755_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15800..16180
                     /locus_tag="JN755_002243"
     CDS             15800..16180
                     /locus_tag="JN755_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381022.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            16246..17070
                     /locus_tag="JN755_002244"
     CDS             16246..17070
                     /locus_tag="JN755_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HAY7381023.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            17198..17734
                     /gene="yfdR"
                     /locus_tag="JN755_002245"
     CDS             17198..17734
                     /gene="yfdR"
                     /locus_tag="JN755_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7381024.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            17725..18087
                     /locus_tag="JN755_002246"
     CDS             17725..18087
                     /locus_tag="JN755_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7381025.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            18087..18392
                     /locus_tag="JN755_002247"
     CDS             18087..18392
                     /locus_tag="JN755_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381026.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            18392..18742
                     /locus_tag="JN755_002248"
     CDS             18392..18742
                     /locus_tag="JN755_002248"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7381027.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            18619..>19439
                     /locus_tag="JN755_002249"
     CDS             18619..>19439
                     /locus_tag="JN755_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7381028.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVY"
BASE COUNT         5094 a         5008 c         4479 g         4858 t
ORIGIN      
        1 gtcactgaca gatttcagac tcagcggtac atcaagacgt tgtttcgtct gcattggcgt
       61 tatccttctg cttaccggct ttactgccat cggagggttt cgtggtcatg ttcatcggtg
      121 tgagatagac atcaccaccg ggacgcggat tcatatcttc caggtcgcgg cagtcattgg
      181 gagagtaaat tccccagtta atcccggtgg cgtaggcttc aaaacgggac ttcatatccc
      241 cgcgcagtaa cgccccggcg ttaaatttgg cgtaataaac gccctgctta ctttttcgta
      301 ccagtccggt gttgatccgc tgttcgatgc gggtcagata cggcaccagt gaatagttga
      361 taaatcccag ccccagctct tcaatattgt tgaaagtggc gcgatcggtg ttctgcacca
      421 tgtgcaacgg cacccggaac agacgacaga tttcttcaag ctgaaacttg cgggtttcca
      481 ggaactggct gtcctcggcg ttcagcgcca tcgacttcca gtccagcccc atctcaagga
      541 tcatcgggcg gtgagcattg ccaagcccgg tgtgacgctc ctcaaaatct ttcttcaggc
      601 gctcgtaagc ctgatctgac agcgtctgct ctgtacgcaa cacacccgac gtcaccgcgc
      661 cattgctgaa cagtctggcc ccgtgctctt cggtcgctgc cgccagcgat attgcctcgc
      721 gggcataggc gatgggattc agccccacca gtccgtccag cgtcagcgtg cgcacatgcc
      781 agatatcctc ctggctcagt acatccgcgg agccatccgg gaatgtgacc tgatagaccg
      841 gctcccagct actgttaagc ttcggtacca cacagctggg atcgacgggc agcagttcag
      901 ccacttcgcc aaatgctttc actttgtagg cgtaaaagtt tccccgcagg cacagacagg
      961 tgaccaccag ctcccagaac tcctgcggcg tcatatagcc attgggatgc gtggagatca
     1021 gcttatgcag acgttcgcca gtggctctct gcttcaggct gccgttcagg tgatacaggt
     1081 tgcagggcaa catcccgacc gactccgcca gcaccctgac acaggaaaaa accgccgtca
     1141 gtcgcatggc ccgctggctg ctgatctgct ttccggtata ggtgtcgtag gacaacccga
     1201 tatcatccgc cagctctgct ggcgtggtca ccggtgcgtc actttttcgt tgaaataatc
     1261 ccgaaaagaa cactatttac ctccgccgac agacgactgt gtacggtcga gatatcgcgc
     1321 caccagccac gaccagaaca ggcacaacgc cccggcaaca acaaaccccg ccgggggata
     1381 aatcagccag gcaccatacg ccagcaaaag cgcccccagc acgcccacca gaggcgcgag
     1441 aatcagcatg atcataatta cctcagttaa agcgagcgga tcccatagga ctcaatgtgg
     1501 tcagacagcg tgtcttcttt ctcgtacagc atggctctgc caaccgccat aatcagcgca
     1561 actgcaccgt caattttgtt ttccgcctgc tctttgacgg gcttcaccac atcatcgtta
     1621 cccggaatgg ttttgccgac cacgttgccg atacactagg tcatgatggg attgccatca
     1681 tgatgaaagc gccccgattc aattgccgct tccagctctt tcatcgggtc ggacatgttg
     1741 gtgtagttct gaatgatagt gatggggttc aggtcttcat cagcaaggtc atgtgacaac
     1801 ccggtcgccc cgaaggggtc gatgggtgac tcactgaccg gactgatttt gttcgccgct
     1861 ttggcctcct cgaggatgta gcgataatcc acctccgcac catcggtaac ggtcagaacg
     1921 cccatttcca cccatttctg aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg
     1981 ctgtacaccg tgtcatacgg tacccagaaa cgcggggcca cactgtagta atgcgtttta
     2041 ccgtcaatct cgcgggtata aagtcgcgcc atgctgttca tatccagctt acgcgccagg
     2101 tcaaaggcca gaatgcacgg ctgcccctcg aactgctcaa gggtcagtga tttatcctcg
     2161 cagctctgcc agctcaccag gttgaaatac gccgaacgcg ccgacaccca gatattgagg
     2221 tgttttgttt taaagacgtt tgccagacgg gcgttatttt tcgcacgctg ctgctgactt
     2281 aacaaaaatt cgcgataaac cgacacgcca atatttggat tggctttttc cagcacctgc
     2341 gggtcggtcc agtcgtcacc ttcatcaacg gtatagataa tcccgaacag ttcatcgttg
     2401 ggtaccgaac cgttgagcat ctcgatgact tcccgccgtt tgtcgtagca cggcccctca
     2461 atgttgtacc cggcggtagt gatagcccac atcagtggct gacgtcgcgc ccccatcccg
     2521 gtaagcatcg tggtgtaaag cgcatctgtg gcgtgctcgt gatattcatc caccaccgca
     2581 cagtggggtg atgaaccatc accggggtta ccaatcagcg gttcaaaccg cgccccatcc
     2641 tccggacggt tcatgtttga ggcgttaacc tcaatcccga acgcttccgt cagcatgggt
     2701 gtgcgtttac acatcagtcg cgccgggcga aagacttccc acgtctgttt ctctgtcgtg
     2761 gcaccggaat acacttccgc gccaaactcg ttatcacagg caaaacaata cagggcaaca
     2821 ccggcagaga ttgccgattt gccgttctta cgggggattt cggtatacac ctcccggaag
     2881 cggcgcagcc gggacccttt attgacccag ccaaacgcac agcagatcac aaagagctgc
     2941 cacggctcca gcgtgatggg catcctcttg aatgcccact cccccttggt gtgtggcaac
     3001 agctgaataa atttcgcggc ccgttcagcc agctccttgt cgaagcggta acgaaacgac
     3061 ttactttttt ccgccatcag gtcatcaaga tggcgctggc aggcctgaat cacaaactgg
     3121 caggccacaa tctttccgcg cacgacatca cgggcatact gattggcagc atttacgttg
     3181 gggtaagatt tccggctcat gactcgatga ttttcagaaa cgggttagtg gctttcttct
     3241 gccccgccag gccaatcaga cgctggcggc tactggggtc gagtccgagc attgcccccg
     3301 tgctgctcat ctcggactcc tgttcttttt tggcggtcag ctccggattt ttgaccatac
     3361 cgcccattgc accggtgatg gtgttgccct gtctggcaat atttttcacg gcacgccgcc
     3421 agaactcata ggccacacac caccgctcaa gcaccgcaag gtcagtcacg cacagcaggc
     3481 cctgaccgca gagttctttg gttgtcagtt gccacatgat cgtggcgaga ggaagctctt
     3541 cttcagcgaa ccactccggt ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt
     3601 tgttcagggc tcgcttgccg gggtttccgg ccagcgcctt gcgcgccgtt ggcttagggc
     3661 gacgcccgga acgccccgcc gttccagcca tatgcggcac tcctggttaa atttcatttt
     3721 tcgcgggtat aaaaaaacga tggggcgggc agtccggaag acgtcaggtc acagagattt
     3781 gacccgcccc tcccctcaga cagttgagaa ttatgatcac tttagccgtt cacgggccgt
     3841 cttctcctta tggcacggcc agcacaggct ctgcagattg ctgtcggcat ctgtgccgcc
     3901 atgcgcttta gggatgatgt ggtcaacagt tttcgcctca cgcaccacac cagcacgcag
     3961 acatagctga cacaggcctt tgtcacgctt caggacacgc acgcggataa catcccactt
     4021 cgaaccataa ccgcgctgat gacgagattg tcctggcttg tattgcttcc agccttcgct
     4081 tttatggctt tcgcagtagc ctgacgggtc cgtggttgta gagcggcagc cgcgaacacg
     4141 gcaggctttt gggattctag ggggcatata aaaaatttat aataagtaaa ataataaaga
     4201 aaaatactca aaggcaatta tcttgactca aatacaaatc tagaagagat gagcttatca
     4261 agcattttca taagtaaatc cttactctca ccaccattta tatatttatt aaagttagac
     4321 tcgaactcat gccaaacttt ttctaactca gtattatcaa ataagtgctc tgtcgcaaac
     4381 catgaagact ggcaacaatc gaaaatactc aaaagttcat caaatctttt aacattctct
     4441 tctcccaact caatttgctg actaataaga tctacatctt taaatagcca tttcgcaata
     4501 acttgctctc gttcatcatt gagtttctcg gggtcaatat tgacaggcat aaataataga
     4561 gctgtcttta atttctttaa tgcattccta aaatctattt ttacttttgt tttctcttgt
     4621 tcccgccacg taaacaatgc cttgaacgcc aaaaccaaag taacaatagt agctccagca
     4681 ctcacccaag atgcaatcat tgcccaacaa gcccactcag cagcagcacg gtttgctaca
     4741 agtgtctcat atgcaatata attttcgttc atttttacct cactgttaat agtgagagta
     4801 ttgtaactaa atgcataata actagaacag tcaaataata atatttcacc taatgaatat
     4861 tattattcaa atgcagattg tacttttgct ctcctaacct acgaagatca gacttatcac
     4921 ggttacatag ccccagtgct gatagcagac ttacattcaa actaagacta tccccataag
     4981 tcagaggatt gggtataact ggctgtggag tttcagcgag caggttcgtc ggtaacggca
     5041 tcgttggaac ctgcacgtat actgtccgcg tacttccgca accggtcagc agcgacatca
     5101 ggcacagggc gtgaagcaca atcatcatcc gcaacagcca ctttgatatc ttcccgggct
     5161 ctctgtgact ccagtgcgat ctgctgtttt gcatgctggt tagcctccag aactgtattg
     5221 acgatttgta gtgattgcag gacgttattg gtaatggcag ttgctgattc agcatttcgt
     5281 acagcctcat cagcacgttt cttttcgtac tgatatttgc tgtagtagtg gttggccgac
     5341 cagatgaaag aaccaatgac agtaacgaag aaaccaacga taagcagctt atagctcagc
     5401 ttcatttacc accccaccag cctctttaaa ccgggcaatc aaatcaccga ttttatgttc
     5461 atactgacca taacctgcac cgggtaacga cgcccagata ttgctgcaac ggtcgatagc
     5521 ctgacgaata tcaccgcgat caatcatcgg taaagcgcca cgctctttaa tctgttgcag
     5581 tgccacagca tcctggcttt tgggggagaa gtctttcaga gcaagctgct tacggtaggc
     5641 atcccaccag cgtgaaagaa gctgataacg tccggcggct gttgatttga gtttggggtt
     5701 tagcgtgaca agtttgcgag ggtgatcaga gtaatcagta aacagttcgc cgccaacaat
     5761 aacatcataa ccgtgattac gtgtcggttg tcgcccgtta tccgttcctt ctgaccatgc
     5821 caccatatcg aggaaagctt tacgctggga atttagtacc tgcataaatt actccttaga
     5881 gccaccaaac ttattaccga ttactctcat tgcagcccca cgaatagcat cgaccccgat
     5941 tagcccaacg ccgccaccaa tggcaacaga aagagattta ggccatccga catactcaag
     6001 agcggatgca aaagtcagcg tcagagcacc acagagcaaa atctcgagcg tttttcgttt
     6061 ccagccccca ccaccgccaa aatatgcaat gcgcaagcca gccataacaa tcgacataat
     6121 cactgcgccc agcggtgtgt ctccacgcca ccagctctgg accaactcca accaggtatt
     6181 tgggttatga ggcatttgta gttatctctc acctcgctga tacagcaggt gcaaattgag
     6241 ggaacatcat gtaccgcaaa tcagaagcgg aaacgtcaaa gagccgaacc aatggataac
     6301 tgcgggatag gccaggccca acgaatcccc agcaacaaaa acgacaaaac ccgctcgacg
     6361 gcgggtttaa gctgtgtggc gaagtaacca ctcttaacag attacaagaa tttttgcgga
     6421 ccgcgttaat gatttttaat cccaaagtcg tattattcgt tttttactgt aggagttaat
     6481 aggtttatat tatgttagat acactatctt tcactgaacg tgacgagttc caacgcagaa
     6541 acatcgctga aaatatcatc aagttgctaa aaccagaggc agacatttca ccactggtaa
     6601 tagacggcgc atggggaaca gggaaaacag aatttagtat caaactgaaa aatctcatta
     6661 ttgagcaaga aactgaatct aaagttgttt atattgatgc ctttaaaggg gatcatgcag
     6721 aatctccatt acttctcata acctctgcaa ttgccagcat tttacctgag gaagaaaaac
     6781 aacacttcat taagagatct cttcctgcaa ttcgctttgg tttaaaaacg gaactaaaag
     6841 ctggtgcagg ttggttttta cgacaggagg ctagtgaagt ttccgaagaa ttccaagatg
     6901 cgatgaagaa agcaagcaat gcagcaatag atgggactat tgaaaatttg cttgaagacc
     6961 atatggaatc agagaaaaac ataaattcac ttaaatcttg catcgaaagc atttcagaaa
     7021 gtcaaaagat agtaattata attgatgaat tagaccgatg taagccgagt ttttcaacaa
     7081 atgtcattga aacaataaaa cacatttttg acatcaataa tgtctttttt attttagtta
     7141 caaacacaga acaattaaaa gcatcgataa atcatattta tggttatagt attaactcac
     7201 aaaagtatct tgacaagttt ataaaatata ccatcacgct tccagacaca tgtttaataa
     7261 atggtcacgt cgtatgtaaa acatctgtta tatattggaa ttaccttgta gacaagacaa
     7321 aatcattaaa tgaaattaat aggttatctg gtagatttat ccgtgattta attcaacgaa
     7381 ccaacttatc cttacgtgaa acacaaactt tttcacgcaa tcttaacatt tttcaactat
     7441 taaacgacga tgagtacaaa agcagtgatc ctttaataaa taagatcttt gtcgtagctg
     7501 tcttcataca ttgctttggt gacaaggaaa aactaaaaca agaaattacc gcagaatcta
     7561 tatctcattt agcagatctg cttaacataa aagaaatacc ttattcttat gagagaagat
     7621 cgcaaattcc tgaaatatct attgtattct tcggaataat taaagacagt attactctta
     7681 atgagcgatt tgcccctaaa agtgatgaag aacttaaaaa attcaccact gtttatactg
     7741 attatgaaca cctaaatttt tggagtacta cacccagaga gttattgata aaatatatta
     7801 atcaaatgtc attcatccag taaataatac gcccctgcat ggggcgtatt attaaacgtc
     7861 caaatctaat gttagatcta gcatggaaag gcagccatca ataaatcctt cggctagctg
     7921 tatttctata cgtatcaatt tctcatcctt tccacgcgcc tttgcaatct tacgcttgga
     7981 tattctgtat aaataatgtg ccacaagaag cgaatgctca taaggtcttt tttgctttag
     8041 acgagcaaga caaccttcaa taattaatgc atcactatcc gaacaagcca agcgtttctt
     8101 gctggtatag ggaagaagtc ccttaaaccc agcagctata ggtgaatagt caacgcctga
     8161 actatcactc gccgcccatg ctccccaacg ctcaagaacc atctgaatat cacgcatcaa
     8221 ctttctccac caaatcaggc cagcacgcca attgccagcg cacgatcgat aaaacgaaat
     8281 atcagctcca gctgggagcc atacttctct tcaaatgcca cggtatccgc atgcagctcg
     8341 tcgtgatgct ttctgcacaa aggcaacaca aagaggtcat gcgcttttgt acccattccc
     8401 ccctgaccgt ggcctatcag gtggtgggga tcatcagcgg gctttccaca acatgcacac
     8461 ggctgtgtct taacccagcg cgtgtacttc tcattaaccc agcggcgacg ttttgggcgt
     8521 aacataaaag actccggcga ctccggatcc actttcagcg ccagcacctt ttttgcctta
     8581 tcttggatga tgctggtggc aggaaccgaa ggcacaaggt cactttcccg ggtgacagac
     8641 ggcacaacag gctttggtaa tctcagtgcc ttacgggctg cactttccgg taaggcatcc
     8701 gccaggtcat tacgaatcag ccaccagcac agttccggca ttgtcacaac gtgactatca
     8761 tcaaaaccga gatcccgacg cacaacagac aacacccagc gggcacagtt atccgttgcc
     8821 attgattcca gccgttccgt gaactgatcg cgcagctggt tatcacagtg ccagcacaga
     8881 cggattgcgc ccggagcgtg tcgcattgtg gtcatgttct cgctgtgcca gtcggaatga
     8941 ggccactggc agcctttttc acgaagtagc cagctttcaa gacattccac gccaccagca
     9001 cgacggatca ctgcctcatt gcggaacacg gcccgaacag caggatcatc cgccagcggt
     9061 tgtgatgccg cgggaacggc accactggcg aaagatgaat aacgttccgg ctcaggctcc
     9121 agcagtacac gcccctgcat aaacaggggc atcagctctg aacctggtct gaacaatacg
     9181 atccccatac gcggggcaat ttcaggggtc agtagtgctc tcacggtcac ctcaatgaac
     9241 ggtatcgagc agctttaaca gctcagggaa tcgggattcg aagaaatgcg gctgcgtctc
     9301 gcgcggattt gcaggactgg taatgttctt gccgaacatg cagcctttcg ccgtcagcga
     9361 ccagaatttt ttgatgttgt taatcgcggt acggctgtat cgttcgcgtt gttcaacgat
     9421 ccccagcttc gccatctggt gatatgcctg attagctgtc aggcggatac catactgttt
     9481 cagcagtgca ctcagcgaca gcgtagggcg acttgagcca tcaggcgcgt cagcaggagc
     9541 atcaatggca tagcgtggtg ccagattcgg taagccaaca gcctcctgga gtttctgaca
     9601 ggccccaagc acagatgagt tagacaggtt taactcccgc cgcataaagt ccagcagaat
     9661 cacgccagcc tgcatcttgt cagcagcctg cccggataat ttttccggtg cgctggttac
     9721 catatcgaaa gtacggatca ccttcagatg gaatgacggg ctgatccaca ttgcataggc
     9781 atacaccagt tctttgcaga catacgtccc ctggttattt ccgccacgac taacgttaac
     9841 tggctctata ttgaccgagt tgcaaatctg caactcgctt attaaacgtt cggtttgctc
     9901 attgcggagc cagaatgcag gcttatgctt atcctgagaa ccggcagccc tgtgcagatc
     9961 gttcaggctg taacgcccat aagcatcacg acgaacttca ataccatcaa tgaccatcag
    10021 attattcata cttcgtttct cctcttaatc aggcggctgc acccgccgtt ttctcgtact
    10081 tattgatagt gatctcgacc ttcccttccg ggataaccgg cccccactcc accagcattc
    10141 ttttcacctg actgtcgtct tcccacacac ccgcgtgggt cagggcgtca aacagcgcct
    10201 tgttatagtt gtccagatcg cggatccggt tatccggagg aaacaacacg atctccactg
    10261 aagcaggtgc cgacgttggt ttcggcagac gacgtaactg ctcaactatt gctgcgcacg
    10321 ccgcgctctg gaattttcgc cccgccgcgc ttatcaggct cttaccagca aacgcccctt
    10381 tgttggggtg tcgccagtac gtgttcacgc tgggcggaaa aggcaggatc agcttcatac
    10441 tttcaggtcc ctctcatgta accagtgggt tgcacgcagc cttgcgtttt cctcaccggc
    10501 aagcagtgcg cggataatcc cgaccgcctc gctgtcgtcg tccttcaccg cggtatgaag
    10561 cgttatcccc cgggccacgc cacgctttat cgtgatgacg ccttttttct ccagtgcgcg
    10621 aagatgctcc accgctgcat tcaccgaacg gtatcccagc atggttgcca cctcctgatt
    10681 ggttggcggg aagccacgtt ctttctgata agaaatcagc atatccagca cctgctgctg
    10741 gcattgagtt aacgtcgtca tgccgccatc tccctgacca gtttttccgc ctgctggcga
    10801 acctgcgcca gaaacgcctc accacatgcc tcaagttcat cgcgcccgat gtagctgatt
    10861 gccggtccct tccaggtctt gtcaaaaaca gcaatagcac cagcgaaaaa agctcctgtc
    10921 agtacctgct tctcgtcttt cgggataaac cagacaggca gttcaaaacc aatacgcccg
    10981 cgaataaaag caatatgatc tgcatcttcc ggccaccaca cttcgctggt ggcagctttg
    11041 atcaggaaaa catagcgccc gcccttatca cgcatggcac tggcatgttt catgatgtaa
    11101 cgcatgccgg tgatgtattg cccctcatgc tgactggcgc ggctgtatgg gggattacca
    11161 aaggcagcac ctttaagctc cgcaagacgt tctgaccagt catgcgccag cgcgttgtct
    11221 tccgccgtgt aatacgcggc acatttggcg ttatcaccgt cagtgaacag atccagaaca
    11281 aacgggccaa acagggtgtt aattccccag aaaatgttgt ccggcgtgcg ccactgatcg
    11341 cccacttcct tcagttcatg ggctggtttg ttccgcagtt ccaccagcgc ctggcaatat
    11401 ttattactca ttaagccccc acgtaattcc ctgacagata ccactcttca cccgatgcag
    11461 cgcgcttgct gcttttccgt aagcaccgct cacgacgcgc cagaaaattg tttcgttctg
    11521 gctgggagtg gctttcacgg aatgccgcca tccacacggt tgcagcacga cggtataagc
    11581 ccctggactc cagttcttcc gcctggcggg tcaggcacaa aatcacacgg ggatcgttag
    11641 tgccgacata gaaattgcgc acaggtctgg tttcacgaac tggttgtggt tccggttcct
    11701 gcgctctctc agtcaggcgc gggaaatgtc tgcgtgtatc tccttcacaa cggtgagcca
    11761 cacgcccact ctgacgtaac ttgcttgctg actgcagaac gcgctgccgt gagtaaccgg
    11821 caaaagcatc cgcaatatct ccggaagtac agccccgatg ggcttcaatg aatttctgaa
    11881 cgtcattcaa aagactcatg ctcaccccct gaatcctgcc gggatttggc tgtagtccac
    11941 gttgtcgtaa ctagctttga agtacgggtc ttcgcgtttt tcggtgtacg tgctgacgga
    12001 cggcgataag cgcagggaaa gctcatccca tttttcccgc agcttcgacg ggctgagcac
    12061 gttacggcac cagaacggat cgcgactgac gcggctgtac atctcgcaga tttgtttatg
    12121 agtacgacca tcctgcacac acatcaggcg aatttcgttt gcccaggctg tccagttcgg
    12181 ttctttggga cgaaccacct cgccgtcaca ttcggcggcc tgctcgtaca gggcgatgat
    12241 ttttttccag agccactgtg cgcaggtcaa atcatcctgc gttccccact ggcgcttttt
    12301 agggctgaat acaaccgcat caggatggcg agttaaaaac tcctgttcag ccgtctgcgt
    12361 gtccggttgc gaagcgtccg gacgagaagt ttttttatct gacggatcat gttttgattt
    12421 tactgacgga tccccgccag attctgacgg gtgaaaaccc gcttttttgc cagatttcga
    12481 cgcatcaaat tttgacgggt cagattttga tgcgtcagat tttgacgggt cagaatctga
    12541 cagttgagaa aatgccgctg cctgaagctt cgcaacgtta agctgataaa cattcgacgc
    12601 attgcggtta ccctggcgac gcgccttacg cgttaaccag ccttctgctt ccagccgtgc
    12661 gatagccgtt ctgacggtac tcattcccgc gccaatctgg cgggcaatgg tttcaattga
    12721 tggccagcac acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct
    12781 ggataatttc atgcctgatg cagcgcaacc atcccataca tagccggtta atttagtgct
    12841 catgaccgac ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact
    12901 catgctcata gccttcgcgg aggtagataa cacgttgtgt ttccggctcc caacgaatga
    12961 ctctgacggg cactccgtag tgctctttga accagcggtt aacttgtcgc aaaggactgt
    13021 ctccttctgc cggttgaaat cacccacagc ccactctgca aagctgtggg ttacaatttc
    13081 cctgtcacct ggtacattta ctgcatagca atactccacc ttcgcttttc cacccggtac
    13141 aggaagcgca atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt
    13201 tctccacagt cacgacatgc cacggcgccc ggagctgcac actcgcgggc gtcactactt
    13261 tctgaaacgc aaaagatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag
    13321 aggtactgct catcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcaatt
    13381 gctgaacgaa tctgttttga ataactgccg atctgttcaa tgacttccag caggcgttgg
    13441 ttgatatcgg cgttgtccac atcctcgacg tcaggaagag acacaaagac gccatttgca
    13501 gactgcgcca cagcatcagc aatgaagtga gtgccaccag cacgctgtaa aaccattgcc
    13561 catcccagcg ggaaaatctg atcgccatct gcacgaaggc ggttgaataa agcgttttct
    13621 gttacatcga gccagtcagc cgcttcagcg taaccacccg gcaacgccgc gatagttttt
    13681 ctgacagctt tcacgtacca ctcaggctgt ttttctattt tccagtgatg cttacccacg
    13741 gttagcctca tcgttctgtg gttaaaaatt gaaagtgttc tgctaatctt tcggatagat
    13801 atccggtctt aagtcagatt tcgtaattgc acctgacgtg cattgctcaa gttttttagc
    13861 cagcacaaaa ctggcttttt tatagccatt gaaaaccagc cgtaagtagc caggtgttga
    13921 gccaactttt ccggccaact cgccctgctg ttctttggtt aaagagtccc aatacgcttt
    13981 catacaatat gtacctccgg tgtacatgtt acatgattga aatgaacctc caagacactt
    14041 gtaccttaac ggtacaaggg ttttaatttc gttatgaaaa caatccatga catccggcgg
    14101 tctaacgcca gaaaactgag agatggtgtt ggcgggaatt cttcctttgc cactatgatt
    14161 gatcgcgagc caacccagac cagcaggttt atgggagatg gtgctactaa aaatatcggt
    14221 gacagcatgg cacgacacat cgaaaaatgt ttcgacctgc ctgtcggatg gctcgatcaa
    14281 gaacaccaga caacgaacat cacaaaaaaa cctgatgttt caatcactaa taaacaaatc
    14341 acattagtcc ctgtcatatc atgggtacag gccggagcat ggaaagaagt tggatattct
    14401 gaggttgatt tgagcacagc agaaacgtat ccctgccctg taccctgtgg ggaaatgact
    14461 tatatcttgc gggtgatagg tgattcaatg attgatgagt accgcccggg agacatgatt
    14521 tttgtcgatc ctgaagtacc tgcctgccac ggtgacgacg ttattgcatt gatgcacgat
    14581 acaggcgaaa ccaccttcaa aaggttgata gaagatggga cacagcgtta tctcaaagcg
    14641 ttaaacccaa actggcctga gccttacatt aagatcaacg gtaattgctc tataattggt
    14701 acagtgattt tctcaggaaa accaagaaga tacaaaatca aagcctaatc aatgtttatg
    14761 aacctgcttc ggcaggtttt tttatacttg acaatgtacc cttgggatac ataatgtacc
    14821 caagcgaaac aacgaacagg caggacgccc acgaagtagc cgcctggggc atatgaagtc
    14881 caggatgatt cgttgagtca tgttgtgcca ctaggcactc atgttaaagc aggtgtatga
    14941 aatgaaagtc cagattttaa acaataactg tgaagtcgtt tggtcatacg acatagccgc
    15001 ccctgtagat cagagcggcg atagctggac caatgggaaa catcagatta tggctggagt
    15061 tgtgttctct ttacgccgtg ctttggaaca ggctgaagta tttccatcag accctgaatg
    15121 gaaatggcct ttttctattt gtccaaattc ggagagcaca tttcagaaaa ttggtcagaa
    15181 agtcgcactc gaagagcatc agccaactgt ttcctgattt tttcaggtaa ctcgtcggca
    15241 tcgcagaaac aacaacgctc gatcatgttg aaagccgatt cgtagaactg tttctgctga
    15301 gtgtcgctga gacaggaaaa gagcgacgtt acgatgattt tattaattgc attatcaagt
    15361 tctttttcat caaaagtcat ttgattttcc ttttatgtat acgggcttaa aaggatacca
    15421 ccgagcctga agtggtgaaa agacaggcac ataacagcta agtattttca accaaagaga
    15481 atccttagag ttgtggtgaa tgcggctcag cgcacgcggg ttaaggttga ggctgacagt
    15541 cgaccttctg tggataccca cccgtctggt gtgcaacctt cgccaggcac cgggaggcac
    15601 ccggcaccac aactttatgc tgtgtgtagt cctggcggta ccagtttgta cccttgcttc
    15661 cggctggtac cgtccttttt acaaaacaga gaagagcatc accggacgac gggctcataa
    15721 cccaatccat ccgggcggct gctaccgcag gtgttcttct ctgttttgtg gagaaactaa
    15781 tcggccttgc agggtcgata tgatgaggag cagcaaaatg gctagcgaac gcagtactga
    15841 tgtgcaggca tttatcgggg agctggacgg cggcgtattt gaaaccaaaa tcggcgcagt
    15901 tctcagtgaa gtcgcttccg gtgtgatgaa cacgaaaacc aaaggtaagg tctcactcaa
    15961 cctggaaatc gaaccatttg atgagaaccg tgtgaaaatc aaacacaaac tctcatatgt
    16021 tcgcccgact aaccgcggga aaatttccga agaagacacc accgaaacgc cgatgtatgt
    16081 caatcgcggt ggtcgcctga ctattctgca ggaagaccag ggacaattac tgactcttgc
    16141 cggtgaacct gacggaaaac tccgcgcagc aggtcattaa tatcgttctt aattaactga
    16201 ttatttatct catcactgaa tatctttata tagttaggac ttattatgtc tcagaactta
    16261 gacgcaaccg caattaatca aatccatgcc cttatttctg ctcagggtgt taatgaaatt
    16321 atcagtaaga ttggtgccga tgctgtggca ttgcctgaga atttccgcat tcatgatctg
    16381 gaaaaattta atttaaatcg cttccgtttc cgtggtgcgc tttccactgc cagcatcgat
    16441 gactttaccc gttattctaa agatcttgca gatgaaggca cccgctgctt tatcgatgct
    16501 gataatatgc gtgccgtcag tgtgcttaac ctgggtacta ttgatgaacc aggtcacgca
    16561 gataacaccg ccactctcaa actgaaaaag acagcaccgt tctctgctct gttgtctgtt
    16621 aatggcgagc gtaactccca gaaatcactg gcagaatgga ttgaagactg ggccgactac
    16681 cttgtgggct ttgatgctaa tggtgacacc attcaggcaa caaaagcggc tgcggcggtc
    16741 cgtaaaatca cgattgaagc aaaccagacc gctgattttg aagataatga cttcagcggc
    16801 aaacgctccc tgatggagtc tgtcgaagcg aagaccaaag acattatgcc agtggcattt
    16861 gaatttaaat gcattccgtt tgaaggtctg aaagaacgtc cgtttaaatt acgcctcagc
    16921 attatcactg gcgatcgtcc ggtactggtt ctgcgcatta ttcagctgga ggcggtgcag
    16981 gaagaaatgg ctaacgaatt tcgtgatctg cttgttgaga aattcaagga cagcaaagta
    17041 gaaaccttta ttggtacttt caccgcctga tttcattact gcaaatgccc ctgcgggggc
    17101 atttatggaa acgtaattta ctcaataatc gccggatggt gagggattct ttttaccaga
    17161 attcagcgcg gtgcagcgca tatacgtgga gaacaaaatg tcatttatta aaactttttc
    17221 cgggaagcat ttttattatg acaggataaa taaagacgac atcgatatta acgatatcgc
    17281 ggtttccctt tcaaatatct gtcgctttgc cggtcatctt tcgcacttct acagcgtcgc
    17341 ccaacatgcg gttctttgca gccagctggt gccgcaggaa tttgcttttg aagcgttaat
    17401 gcatgatgca acagaagcgt attgccagga cattcccgca ccactgaaac gccttcttcc
    17461 tgactataaa caaatggaag aaaaaataga ctccgtaatc cgtgagaaat acggattacc
    17521 cccagttatg agtacgcccg tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg
    17581 cgatctcggg cttgatgatg gctctttctg gcctgtactg gaaggtatcc cggcaacaga
    17641 gatgttcaac gtgattccac tggcaccggg ccatgcctac gggatgttta tggaacgctt
    17701 taacgagtta tcggagttac gcaaatgcgc atgaatgttt tcgaaatgga agggtttctt
    17761 cgtgggagat gtgtaccgcg agatctgaaa gtaaatgaaa cagatgctga atacctggtg
    17821 cgtaaattcg atgcgcttga agctaaatgt gcagcacagg aaaacaaagt aataccagtg
    17881 tcaactgaac tgccaccagc aaatgaaagt gttttgttat tcgatgctaa cggagaaggc
    17941 tggctaattg gttggcgttc tctctggtac acctggggac aaaaagaaac cggagaatgg
    18001 cagtggacat ttcaggtcgg ggaccttgaa aacgtcaata tcactcactg ggcagtaatg
    18061 ccaaaagcac cggaggctgg agcataatga ccacttttac cgacaaagaa ctgattaaag
    18121 aaattaaaga gcgtatcagc agccttgacg tgcgagacga tattgagcgc cgtgcttatg
    18181 aaatcgcact cctatctctg gaagtagaac cagatgaacg cgaagcttat gaattattca
    18241 tggaaaagcg tttcggtgac ttagtagatc gtcggagagc aaaaaacggc aataacgaat
    18301 acatggcatg ggatatgact ctcggttgga tcgtctggca gcaacgagct ggtatccatt
    18361 tttcaacaat gtcacagcaa gaggtgaaat aatggagcca tacagcctca cactcgatga
    18421 ggcctgtcat tttctcaaga tatccagacc gactgccatt aactggatac gcacagggcg
    18481 tcttcaggca acacgcaaag atcccactaa gaataaatct ccttacctca caacacgaca
    18541 agcctgcatt gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg
    18601 cataacagag gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc
    18661 acattgccga acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg
    18721 acagaactcc atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata
    18781 cctgatataa cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa
    18841 tcactggacg atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatgcaa
    18901 ctaagagaca ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt
    18961 catgaggaaa actggaaact cagggcagaa gcatgcagaa aaaaagggaa acctgttcca
    19021 gaatacacgc caaaaccagc gtccgttgta acgaaggcta cgcatctttc atttataaag
    19081 gccctactaa gagccgcaga gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa
    19141 gtgcctcaac caaagaataa acggatccgc tggctggagc cccatgaagc acaaaggctg
    19201 attgatgaat gtccggagcc attaaagtct gttgttgaat ttgcactggc aacaggctta
    19261 agacgctcga acatcatcaa ccttgaatgg caacaaatag atatgcagcg ccgggtggca
    19321 tggataaacc cggaagagag taaatcaaac cgcgcaatcg gcgttgcgct gaatgatact
    19381 gcatgtcgcg ttttgaaaaa acaaatcgga aatcatcacc gttgggtatt tgtgtacaa
//
LOCUS       DADLTI010000071        19389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLTI010000071 DADLTI010000000
VERSION     DADLTI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.071"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JN755_002250"
     CDS             complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JN755_002250"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7381029.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JN755_002251"
     CDS             complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JN755_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7381030.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="JN755_002252"
     CDS             complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="JN755_002252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7381031.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="JN755_002253"
     CDS             complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="JN755_002253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7381032.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="JN755_002254"
     CDS             complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="JN755_002254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7381033.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="JN755_002255"
     CDS             complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="JN755_002255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7381034.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="JN755_002256"
     CDS             complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="JN755_002256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7381035.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="JN755_002257"
     CDS             complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="JN755_002257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7381036.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="JN755_002258"
     CDS             complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="JN755_002258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7381037.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="JN755_002259"
     CDS             complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="JN755_002259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7381038.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="JN755_002260"
     CDS             complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="JN755_002260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7381039.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="JN755_002261"
     CDS             complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="JN755_002261"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7381040.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="JN755_002262"
     CDS             complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="JN755_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7381041.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="JN755_002263"
     CDS             complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="JN755_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7381042.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="JN755_002264"
     CDS             complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="JN755_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7381043.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15358..16350
                     /gene="asnA"
                     /locus_tag="JN755_002265"
     CDS             15358..16350
                     /gene="asnA"
                     /locus_tag="JN755_002265"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7381044.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="JN755_002266"
                     /pseudo
     CDS             complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="JN755_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="JN755_002267"
     CDS             complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="JN755_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7381045.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4631 a         5126 c         4865 g         4767 t
ORIGIN      
        1 gcattacacc ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
       61 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
      121 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
      181 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
      241 caattgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
      301 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
      361 tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa
      421 tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt
      481 gtcagagaaa tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat
      541 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
      601 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
      661 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
      721 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
      781 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
      841 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
      901 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
      961 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
     1021 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt
     1081 tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
     1141 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
     1201 atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat
     1261 aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat
     1321 acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg
     1381 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
     1441 ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt
     1501 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
     1561 gttttcgatg atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt
     1621 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
     1681 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
     1741 agtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc
     1801 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
     1861 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
     1921 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
     1981 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
     2041 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
     2101 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
     2161 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
     2221 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
     2281 ttatcgccaa tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc
     2341 agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca
     2401 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc
     2461 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
     2521 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
     2581 tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
     2641 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
     2701 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
     2761 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
     2821 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg
     2881 gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
     2941 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
     3001 tcatccagtt tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc
     3061 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
     3121 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
     3181 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
     3241 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
     3301 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
     3361 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
     3421 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
     3481 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
     3541 accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
     3601 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat
     3661 cagcaggatc tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg
     3721 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
     3781 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
     3841 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
     3901 tcgtcgagat cctggccgcg aattgcagtg tcggccagaa cggtcacgtt gccaggctgc
     3961 acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg
     4021 cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt
     4081 tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct
     4141 gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa
     4201 gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg
     4261 gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg
     4321 agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga
     4381 agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca
     4441 tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc
     4501 cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg
     4561 agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca
     4621 cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag
     4681 gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct
     4741 gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc
     4801 ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca
     4861 gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa
     4921 cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga
     4981 taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta
     5041 cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca
     5101 tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga
     5161 acggacacat caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca
     5221 gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc
     5281 ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta
     5341 ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg
     5401 aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca
     5461 ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct
     5521 gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct
     5581 taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag
     5641 ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag
     5701 gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc
     5761 caggttttca accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc
     5821 cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc
     5881 atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt
     5941 aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa
     6001 catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac
     6061 ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat
     6121 tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt
     6181 gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc
     6241 cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc
     6301 aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc
     6361 ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt
     6421 gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc
     6481 aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg
     6541 aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg
     6601 ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg
     6661 aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga
     6721 accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc
     6781 actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga
     6841 gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt
     6901 ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc
     6961 gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg
     7021 gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc
     7081 agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat
     7141 tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc
     7201 gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca
     7261 gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat
     7321 tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg
     7381 gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg
     7441 ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg
     7501 cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg
     7561 atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc
     7621 tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca
     7681 gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc
     7741 acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact
     7801 ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc
     7861 tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata
     7921 caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg
     7981 ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt
     8041 tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc
     8101 tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct
     8161 gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc
     8221 atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct
     8281 acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg
     8341 agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc
     8401 agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca
     8461 gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc
     8521 tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg
     8581 cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt
     8641 tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc
     8701 cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg
     8761 cttcaatttc agcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt
     8821 tcgcttcgtc cagaatctgc gagcggagtt tattcgcctg ctcgatgatt acctgggctt
     8881 ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat
     8941 gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg
     9001 ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg
     9061 cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc
     9121 tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga
     9181 cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga
     9241 gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg
     9301 aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc
     9361 atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa
     9421 cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag
     9481 aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac
     9541 attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc
     9601 accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct
     9661 taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt
     9721 caactctttc gtgaagccgc cgatgccttt cattttgatg ttgtagaaca gaatcaggat
     9781 aaatacgccc agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag
     9841 tgcaggcaga cccagtacat gttcagcaat gtacggcagc aagtcgatag gcagtaaatc
     9901 catcagattc atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt
     9961 gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat
    10021 cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa
    10081 aaccaagaac aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca
    10141 gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag
    10201 gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc
    10261 ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc
    10321 cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac
    10381 accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaagc cacccggcct
    10441 ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga
    10501 aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac
    10561 agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt
    10621 cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc
    10681 gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata
    10741 cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat
    10801 ttttccagtg catacaattg cgacttttcc gcgacccctg ttcgatcatg aaaaactgta
    10861 aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg
    10921 cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta
    10981 aaaacagata ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt
    11041 taataatgtt ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt
    11101 tcactaatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag
    11161 agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca
    11221 gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat
    11281 caccaccaga tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac
    11341 ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta
    11401 gaaacggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc
    11461 aaaagcgcgg ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct
    11521 ctgtactggt tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac
    11581 gcgtttacca aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg
    11641 aatgcctggc agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg
    11701 tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga
    11761 agtcaggttg tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt
    11821 tttctggtga tcagtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag
    11881 cacggtgatt acctgttctt gatgcgttgc ttggtaagcg ggtgcttacc aggcattttt
    11941 aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga
    12001 gatggccgca ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ccggtttgtg
    12061 atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt
    12121 cgcgggtagc agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc
    12181 gatataacct tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa
    12241 cgcaggggca aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag
    12301 cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc
    12361 tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat
    12421 attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg
    12481 accgatttca gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc
    12541 gcgcgaagta aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag
    12601 tacgccgaga tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg
    12661 ggcagcgtta agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc
    12721 gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag
    12781 gtcgcgagga tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt
    12841 ttccatcccc tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga
    12901 gataccgttc ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg
    12961 ctgatttctg tctgcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc
    13021 gacaccttcg atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc
    13081 atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat gcagggacgc
    13141 attgcccata aacgagaata ccggcattgg gttatcgcca tgttgttgcg ccagcacgct
    13201 aaagtcgatg gttcgagcat caatacgcgg cggagtaccg gttttcagac gaccaacgcg
    13261 cagcggcagt tcacgcaaac ggcgagagag cggaatggat ggcggatcgc cagcacggcc
    13321 accgctgtaa ttatccagac cgatatgaat tttgccgtcg aggaacgtcc caacggtgag
    13381 caccacagct ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc
    13441 gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc
    13501 cagcgccgta cgtaccgcct gacggtagcg cacacgatcc gcctgagctc gggtagcgcg
    13561 aaccgccggt cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc
    13621 tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc
    13681 gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag
    13741 agtctgttga cccatacgcg ccgcagccat cgcggcctcg gtgcctgcat gacccccgcc
    13801 aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt
    13861 atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga
    13921 cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat
    13981 ctcttattag gatcgcactg ccctgtggat aacaaggatc cgacttttaa gatcaacaac
    14041 ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag
    14101 aatgaggggt tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg
    14161 ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt
    14221 gagtaaatta atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc
    14281 aagaatgttg atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc
    14341 ctcgagttta tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc
    14401 gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga
    14461 aaggttgtcc ggaatatctc cggcaccgtg ggtggagctg ataaccagcc agatccccga
    14521 ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc
    14581 cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat
    14641 aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga
    14701 acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgatg
    14761 gtacgcatga tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt
    14821 gtttggatct tgttgataag tacatgctgg agagcgtcga tcgaacggca cataactttt
    14881 ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc
    14941 agctttgcca gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag
    15001 cctacgtcat aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc
    15061 gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc
    15121 gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc
    15181 agattgtcga tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta
    15241 ttttagcctt cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg
    15301 catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg
    15361 aaaaccgctt acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc
    15421 caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg
    15481 gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct
    15541 ctgcctgatg cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta
    15601 gggcaacacg acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc
    15661 gatgaagacc gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc
    15721 gtaatgggcg acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg
    15781 gcgggaatta aagcaaccga agctgcggtt aacgaagagt ttggcctggc accgttcctg
    15841 ccggatcaga ttcacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat
    15901 gccaaagggc gtgagcgggc gattgcgaaa gatcttggcg cggtattcct cgtcgggatt
    15961 ggcggcaagt tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta tgatgactgg
    16021 agcaccccgt cagagctggg ccatgcgggc ctgaacggcg atattctggt gtggaacccg
    16081 gtactggaag atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgctg
    16141 aagcatcagc tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg
    16201 ctgctgcgcg gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact
    16261 atgctgctgc tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagttgct
    16321 gttcgcgaga gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc
    16381 ggcttcgcat cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt
    16441 tgccgtgtgc cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt
    16501 tcactttact cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg
    16561 catcggcatc aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg
    16621 caagatcggt gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt
    16681 gtgggcctga aagctcataa cggacgatct cggtggaaaa tagcataata taacagcgcc
    16741 ggttttcagc gagagcaatg cgcatcaaag ccaggcagaa cgctttcgca cactgttcat
    16801 taaagccgcc cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt
    16861 catcgtaatc tttatgtacc accggacgtt cgagcacttt ttcacgccac gactcaccgt
    16921 gcaggcgata ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt
    16981 ccgttatccc tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt
    17041 gcagaccatc aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt
    17101 ccatctacgc atcgttgcgc ggtattgatt tggcttcccg agaacgcccc ggctgctctg
    17161 ccaggcgttt cagttccggc tgttcgttaa gaaattcacc gtatttcaca ataaactgat
    17221 agtcgccacg tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt
    17281 tatctgcgag aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta
    17341 acagttgttc gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca
    17401 gacttaaacg ccaacgctgg agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag
    17461 catcaaccaa ctgccgggct tgttctgccc atggggaatt cagacgatgc agtaagtcta
    17521 ggatctgtgg tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc
    17581 acatcacctc ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg
    17641 cctcacgcca gcggggaaca tcatcagtga ttgccgcctt cagtcgtggg aatttttcaa
    17701 agaagaccgc cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct
    17761 cttcgctgac ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg
    17821 aatctgtgca ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat
    17881 aaacagacat ttttgctgtt cgctgaataa agcgtgttgc ttacgccagt cgctttcaag
    17941 ctcttccagt tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc
    18001 cagcgtactg ccttgtaaac ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc
    18061 cagattcagt ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt
    18121 actcagccac tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg
    18181 cagttttaac ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag
    18241 ttgatactgc tgacgacggc tgaaaatgcc gcccagacga attaacgtta aggctgtttt
    18301 atcgctttgt tgctgctgga gttgcaggtg acgttgcaca atcgcgccca ggcgggtcaa
    18361 catcccttgc tgttgccagg catgaccggt catcaataca tcaatttgtt gttgtatcaa
    18421 attcaggctt tgcgcgtcat accacaggca atctttcagc aaaatgagat caaccggggc
    18481 aacagcactg cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca
    18541 gcgacgatcc gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa
    18601 aatcagctca aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc
    18661 atattcttca tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg
    18721 actggtcagc atggagcgga aattcgcttt atcttgcact ttatctaacc atagacgaat
    18781 cagcatgcgg tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc
    18841 cgccaccagt agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc
    18901 gttaatggcg gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag
    18961 aaagacgatt tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc
    19021 ttttagcgcc tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat
    19081 cagatattca aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact
    19141 tttggcaata cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa
    19201 acacaagcgg atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg
    19261 ggaaattctt tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa
    19321 aagttctgcg taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag
    19381 gggggtaat
//
LOCUS       DADLTI010000072        18732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLTI010000072 DADLTI010000000
VERSION     DADLTI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.072"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN755_002268"
     CDS             complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN755_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7381046.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSG"
     gene            complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN755_002269"
                     /pseudo
     CDS             complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN755_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN755_002270"
     CDS             complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN755_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7381047.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            2985..3086
                     /locus_tag="JN755_002271"
     CDS             2985..3086
                     /locus_tag="JN755_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381048.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN755_002272"
     CDS             complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN755_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7381049.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(3430..4167)
                     /locus_tag="JN755_002273"
     CDS             complement(3430..4167)
                     /locus_tag="JN755_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7381050.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4291..5470)
                     /locus_tag="JN755_002274"
                     /pseudo
     CDS             complement(4291..5470)
                     /locus_tag="JN755_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5662..6654)
                     /gene="proX"
                     /locus_tag="JN755_002275"
     CDS             complement(5662..6654)
                     /gene="proX"
                     /locus_tag="JN755_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7381051.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(6712..7776)
                     /gene="proW"
                     /locus_tag="JN755_002276"
     CDS             complement(6712..7776)
                     /gene="proW"
                     /locus_tag="JN755_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7381052.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(7769..8971)
                     /locus_tag="JN755_002277"
     CDS             complement(7769..8971)
                     /locus_tag="JN755_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7381053.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(9326..10285)
                     /gene="nrdF"
                     /locus_tag="JN755_002278"
     CDS             complement(9326..10285)
                     /gene="nrdF"
                     /locus_tag="JN755_002278"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7381054.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(10295..12439)
                     /gene="nrdE"
                     /locus_tag="JN755_002279"
     CDS             complement(10295..12439)
                     /gene="nrdE"
                     /locus_tag="JN755_002279"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7381055.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(12412..12822)
                     /gene="nrdI"
                     /locus_tag="JN755_002280"
     CDS             complement(12412..12822)
                     /gene="nrdI"
                     /locus_tag="JN755_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7381056.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(12819..13064)
                     /gene="nrdH"
                     /locus_tag="JN755_002281"
     CDS             complement(12819..13064)
                     /gene="nrdH"
                     /locus_tag="JN755_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7381057.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(13312..13641)
                     /locus_tag="JN755_002282"
     CDS             complement(13312..13641)
                     /locus_tag="JN755_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7381058.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            13793..14143
                     /locus_tag="JN755_002283"
     CDS             13793..14143
                     /locus_tag="JN755_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7381059.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            complement(14180..14617)
                     /gene="alaE"
                     /locus_tag="JN755_002284"
     CDS             complement(14180..14617)
                     /gene="alaE"
                     /locus_tag="JN755_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7381060.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            15285..15689
                     /gene="stpA"
                     /locus_tag="JN755_002285"
     CDS             15285..15689
                     /gene="stpA"
                     /locus_tag="JN755_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7381061.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(15736..16260)
                     /gene="ygaP"
                     /locus_tag="JN755_002286"
     CDS             complement(15736..16260)
                     /gene="ygaP"
                     /locus_tag="JN755_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7381062.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(16270..16569)
                     /locus_tag="JN755_002287"
     CDS             complement(16270..16569)
                     /locus_tag="JN755_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7381063.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            16752..16910
                     /locus_tag="JN755_002288"
     CDS             16752..16910
                     /locus_tag="JN755_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7381064.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            16994..17443
                     /gene="kbp"
                     /locus_tag="JN755_002289"
     CDS             16994..17443
                     /gene="kbp"
                     /locus_tag="JN755_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7381065.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(17444..18102)
                     /gene="csiR"
                     /locus_tag="JN755_002290"
                     /pseudo
     CDS             complement(17444..18102)
                     /gene="csiR"
                     /locus_tag="JN755_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(18123..>18732)
                     /locus_tag="JN755_002291"
     CDS             complement(18123..>18732)
                     /locus_tag="JN755_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7381066.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
BASE COUNT         4791 a         4751 c         4722 g         4468 t
ORIGIN      
        1 aaccagacag cgtaatggtg gtcagcggca taaagaagca ggccaccgca aacccctgga
       61 taaactgcgg ccaggccgac gcgccaaaat ccatacctgg ttcaaaggta taggcacgcc
      121 agtagaagca gacggcatac ataataaagc tgaaggttac cagccgccgc atatccagtt
      181 tatgcgcgaa gcggccgata atcggcgaca gaatcaccgg aataatccct accggcgcag
      241 aggccaaacc tgcccaggtc gccgtgtaac cgtagacctc ctgcaacaac tgcggcagca
      301 gaacaatagc gccgaagtag agcatatacg cgaggctgat acacaagcag ccgatggtga
      361 agttgcgcga cttaaacaac gacagatcga ctatcgggtt atcgtcggtc agctcccaga
      421 caatcaggaa gcagatagcc accaccgcca ccacggtaag aatgataatt tcctgtgatg
      481 aaaaacagtc cagctcttta ccgcggtcga gcataatctg caggctgccg ataccaataa
      541 ccagcagtgc cagccccacg gcatcaatcc gccgccgttc ggtgcgggtt tcacgtccgc
      601 gcagagtttg cagtgtcatc aacaccaccg ccacgccaat cggcacgttg atgaagaata
      661 tccagcccca gtggtaatta tcgctgatat aaccgccgag gatcgggccg caaattggcg
      721 cgacaatcac cgtcatcgac cacaacgcca gcgcgatcga gcgtttggct ggcgggtagt
      781 tattcagcaa tagactttgc gaaagcggga tcaacggccc ggcgacaatc ccctgaatca
      841 cgcggaagaa gatcagcata ttcaggctgc tggagacacc acacgcccac gacgcaatag
      901 caaaggcgat ggtggaccaa aggaacagtt tcacttcccc gacgcgcttt gccagccagc
      961 cggtaagcgg gatcgagatg gcattcgcca ccccgaaaga agtgattacc cacgttcctt
     1021 ggctgagcga tgagcccaga ttcccggcga tagtggggat cgccacgtta gcaatggtgg
     1081 agtccagcac ctgcatgaat gtcgccagtg acagcgcaat cgtcataatg accagttgcg
     1141 cgccttccag cggtttttgc tgttgcatca cacgcacctc tggattagcc agcgttagct
     1201 tttacgatat cgtcgatcag tttattgaca ggtgctaggc tgatttcacg cgcggtactt
     1261 accgcgaccg gtgttgaacg tactttattt gccagtacct gaccgtcacg gttagtggta
     1321 ttgacgctca ccagcgtgga caaaccaatg cgcagcgggt attgctccag ctgtttctgg
     1381 tccagttcga tacgcacagg tagacgctga acgactttga tccagttacc ggtcgcattt
     1441 tgcgccggaa gcagtgagaa cgcgctacct gtgcccatat ccagaccaac cactttaccg
     1501 gtgtatttca catcatcgcc gtaaatatcg gtggtgatgg tgaccggctg accgatgcgc
     1561 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
     1621 accgccatca gcggcgtcgt tgggctaatt tgcgcacccg gttgcacggc acgacgggaa
     1681 acataaccgg tcatcggact gacaatacgg gtacgctcca gcgccagcta ggcgttacgt
     1741 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
     1801 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
     1861 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
     1921 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tttgcacctc aatattcgcc
     1981 tgcaactgtt tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
     2041 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
     2101 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
     2161 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
     2221 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
     2281 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
     2341 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
     2401 gcttgccaaa aaggccagca tgttgcgatg ctggccggtc attttttctt ttataaatct
     2461 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     2521 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     2581 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     2641 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     2701 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     2761 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     2821 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     2881 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     2941 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     3001 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtga
     3061 caaataaagt aaatgtattg ttttagaaaa atgattcttg tgggttatat aatcgccatc
     3121 actttccagg cgagcccata ggccagcgca ctaagcagtg ttgggatgat aatgctgcgt
     3181 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     3241 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     3301 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     3361 cgcaggcgca gcggcaaata gcggaagcaa taattcgccg cgccaaccag taacccaagc
     3421 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     3481 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     3541 aagggcacca actaaagctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     3601 caggaaactc ataaagagtg ccggaagcat aaaacctaat gcagcttcaa cggcgggata
     3661 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     3721 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     3781 cagttttgcg gtggcggcgg caaaaacctc atccgtcagg ccaaacgccc atagggcagt
     3841 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaaaacatg
     3901 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     3961 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     4021 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     4081 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccagg
     4141 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     4201 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     4261 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     4321 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     4381 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggcggcac cgccaataaa
     4441 gtagctggtc atgtaacctg cggtcaggcg attacgcgca tcaggatgta ttcgataaat
     4501 taccgtctgg ttagtgatat gcacgccctg tacggtgagg tccagcacca gaatgccgat
     4561 aatcaacgcc agtacggaag tgtgaccaaa ccagattgcc agccatgaaa gtaacagcag
     4621 aagcagaccg aaagttgtgg tgcgatttgc ccttgtcggc aaaaccgccc gccggacgag
     4681 cgcccaacgc tccggcagct cccgcaagcc caaacagacc aatgtcacca tcgctgtagt
     4741 taaaaggtgg agcggcaagc aaaaaggcca ttgaggtcca gagaatgctg aagttggcaa
     4801 aggtcatgca gcccagcaac gcgcgggtgc gcaggatctt atcgctgatg aacatgctga
     4861 aaacggaacc caacaactgt gggtagttga ggtgggtttc tgatttcatt tgtggcagac
     4921 cacgccataa tgccagcgcc atcagtgcca ttaacaccga agcaacccaa aagacggtgc
     4981 gccagccgcc gaggtttgcc agtaacccgg caacggttcg tgccagcaat atccccaaca
     5041 gcagaccgct cataatggtg ccaaccactt tgccgcgttt atccggtgaa gccagcgtcg
     5101 ctgccagcgg aaccagaatt tgtgccacga ctgagaataa accggttaat gccgtaccga
     5161 ggatcatcat cgccagtgac tgactgctgg cagtgattaa cataccgccc gctgccagta
     5221 aggtcatcga gacaatcagg cggcggcgtt caaacatatc accgagggga acaagaaaca
     5281 gtagacctgc ggcatagccc aactgcgcgg cggtaacaat aaagcctgcc gaactggcgg
     5341 aaagggaaaa gttacgcgcg atggtgtcga gcaatggctg ggcgtaatag ttactggcta
     5401 ccgccagacc ggtggcgata gacatcagca cgatcagcgc cgggctaagc tcatgattag
     5461 gtttagtcat tatttttgtt catcaggtga tgattaagct ggaaatcaat aatagcggat
     5521 gttgatgaat atgggatgaa agatagcttg ttgaaatgtg cgatgtggtt tgtaggcatg
     5581 ataggacgcg tcagcgtcgc atcaggcatt gtgtacagag cggcggatga ggcgaaaacg
     5641 ccttatccgc ccgaataaaa attacttctg cgctgccagc gcctcattca cccagccatc
     5701 gaactgctgc tggtgggctt tgatccagcc atcaacgtgg ccctgaatat cgccttctga
     5761 ggctttgccg tcatgcataa tggcgttctg ggcgttaata tcggccactg gcaactgcat
     5821 aatggcaaac agtttggctg ccgccggatt tttctcggtc caggctttgt tggcaacgat
     5881 atgcatggtg ctgaccggga agccgtaatt cgcgccattc ggcagtttgg tatcggcatt
     5941 tttatcgccc ggcagtgcgg agaacggcac ctgcaaccag accacatctt tccctggctt
     6001 cagctcatta ctcacccagt acggcgtcca ggtgtaataa aacaccggtt tgccctcttt
     6061 gtagcgactg atggtgtcgg ccatcatcgc tgcgtagttc ccctgattat gcgtcacggt
     6121 gttggtcagt tcatacgcgg caagctggtg gttgatcgca ccttcgcagc cccagccagg
     6181 gttacaaccg gttaaatccg ctttaccgtc gccgttggta tcgaacagtt tggcgatctt
     6241 cggatctttc agttgtgcga tgttggtgat tttgtattgg tcggcggttt tcttatcgat
     6301 caggtaaccc tgtgctgcgc cgttaacaaa taccccttca cgataaaatt tcttatcgcc
     6361 accggcagct tcgtacatgt tgtcatggag tggcgtccag ttcacggcgg tgaaggttgc
     6421 atcgccggaa gcaagcgagg tgtagccaac gttgtaatcc acttcgctgg gtttgttgac
     6481 ggtataacct aatttctcca gcgcacgact gaccagcagc gtctggaagg tttcttcagt
     6541 gatggtgctc tgaactggat taacagtaat gcctttgccc ggcagatcgg cagcaaaagt
     6601 ttgtgtagag ataagcgtgg caaacgctgt cgcaaaaagt acgctatgtc gcattgttat
     6661 tccttttttt ggcagggtga taatgcccgg cgtcaccggg caagtgcaga gttacttaat
     6721 gaatgggcgg gtcagcagac caacagggcc agtggtgtac cagcgacggt tgccgcgact
     6781 gcgtgagtcg cgcccaacgg cctgcgtcag gcgatcgagg ataatggcga ggatcacaat
     6841 cccgacgccg ccaacggtgg caagccccat atccagacga ccgataccgc gaagtaccat
     6901 ctgacccagc ccgccgacgg caatcatcga ggcaatgacc accatagaaa gggccagcat
     6961 cagcgtctgg ttaacgcccg ccataatggt cggcatcgcc agcggtaact gaactttgaa
     7021 cagcatctgg cgcgggctgg caccgaatga gcgcgaggct tcaatcagat ccgccggaac
     7081 ctggttaatc cccagaatgg tcagacggat aatcggcggc agcgcaaaga tgatcgtcac
     7141 caccacgccc ggcacgttac cgataccaaa tagcatgacg attggcacca gataaacaaa
     7201 cgctggcgtg gtctgcatgg catcaagcag tggacgaata attttcgccg ctcgcggact
     7261 tctcgccagc catatcccca acggcaaacc gatgacgata cagaacagca gggcagttaa
     7321 caccagcgcc agagtcacca ttgcctgcga acaggcaccg attgcgccga tggcaatcag
     7381 cgagaccagc gtcgccacgc ccattccgac cccggaaatc tgccaggcga tgagagcgaa
     7441 aacgataatc gccactggcg ctggcatacc cagcagcaat tgctggaaac cgttgaggat
     7501 ataatcaacc ggaacgcgca cgccctggaa gacgggacgg aaatgggtaa caacccagtc
     7561 gatcccttca gtgacccaac tgtcgagcgg gatcagcgtt ttatggaacg gatcgagaat
     7621 attaaaatgc tcgacgtttg gcgcaggcgt actggtcagc cagtcagcac caccgccgtc
     7681 agccggtgca gtcgtcggtg tcccccaggc gtctgcggat tgcgcggcac tgtccgccgc
     7741 tggcgtggta tcccacggat tattttgatc agccattatt taccccctca cgatctaaag
     7801 cgcgcagcag cattcctttc gaaatgatgc cgacatactg ttggtcctcg tcgaccacgg
     7861 gcaccgcaca gggtgcctgt ccgacatgag agagcaactc gctaagaggc gtttgtgcat
     7921 cgactgctaa cggcgcatca atcagcgccg catcaagacc ttgctgctgc gttaacgcgg
     7981 ttttaagcga atcgatggag actgcgccga caaacttatt accgcgttcg ataacgtagc
     8041 catattcgcg atcttcatcc tgcaataatt tcagtgccga acgtgggccg aagccaggag
     8101 ttttacgaat taagccattc ggtgtccggc gggcaatatc tttcgcactg aacacctgac
     8161 taatatcaac gccacggaag aaggtacgga cataatcatt cgccggatta ttgagaattt
     8221 catccggtgt gccgacctgt accacttcac cattttgcat aatggcaatt cggtcgccaa
     8281 tacgcatggc ttcatcaaga tcgtgggaaa taaagacaat ggtgcgctga tgtttcgcct
     8341 gtaattttac cagctcatcc tgcatctcgg tgcgaattaa tggatcgagc gccgagaagg
     8401 cttcgtccat taataatata tccggattaa tcgctaacgc gcgggctaat cccacacgtt
     8461 gacgcatccc gccagagagt tcatccgggt agctgtgggc ataattttcc agcccgacct
     8521 gacgcagtgc atcaagggct ttttcccggc gttcttcggc attaattccg gccaattcca
     8581 taccgaacgc agtattgtcc agcacggtca tatgcggcat taaggcaaag gactggaaga
     8641 ccatcgcaat cttttttctg cgcacctcac ggagttcggc gtcggatatt ttggcaatat
     8701 ccacaccatc aatcagcact tgcccgcggg tgggttcaat caggcgattg agaaggcgta
     8761 ccattgtgga tttacccgag ccggataatc ccatgatgac aaatatctcg ccttcttcaa
     8821 tggccagact ggcgtctttt acgccaagcg atagcccagt tttttccaga atttgttctt
     8881 ttgaaagtcc ttgttcgata tatttgaacg ctcgctgtgg atgctcacca aatattttat
     8941 aaagattttt aatttctaat ttaatggcca tgcaatagaa tgattccttt atttgtttat
     9001 gtcgatatat tacccaatgg gaatagtcac ttttttctac cctaacatac tgagaatctg
     9061 aggcaacccc tgagggcaaa tgtggcatga atattgatgc gagcaaatcg ccggaatttt
     9121 ccgtgatata agggctgaga gcaaatcgag aaaagttgat attttttgtc atagatagcg
     9181 aaaaaacgcc tgaatattgt agtttcaggc gaataatgca acagagtatc agtgtagatc
     9241 actacagaat atttgcgtga taaaaagcta tttggcggga attatttccc tgctgcgggt
     9301 agtgatattt ttgaaaataa caccctcaga aattccagtc ttcatcttct gtttcaaccg
     9361 ctttccccat cacataagag gagcctgaac cggaaaagaa atcgtgattt tcatcggcat
     9421 tcggcgaaag cgcggcgagg attgccggat tcacttccgc catttctgcg ggaaataacg
     9481 gttcgtagcc cagattcatc aaagccttat tggtgttgta acagagaaac gctttcacat
     9541 cgtcagccca cggggtttcg gcgtacagct catcggtgta ttgcaactcg ttgtcgtaga
     9601 gttccagcaa caaatcgaag gcgaaactct tcaactcttc acgttgtccc agagatatct
     9661 tttccatgtt tttctgatat ttatagccta tgtagtaacc gtggactgct tcatcgcgga
     9721 taatcagacg gatcaggtcc gcggtattgg tcagctttcc gcggctggaa aaatacatcg
     9781 gcagccagaa accggaatag aacaaaaaag attcaagaaa cacactggcg attttctttt
     9841 tcagcggatc atcaccgcga taatgttgct gaataatctg agcttttcgc tgcaacggtg
     9901 cgttttcttc actccaggcg taggcggcat cgacatcttt ggtctggcac agcgtcgaga
     9961 aaatcgaact gtaagagcgg gcatgaaccg cttccataaa gctgatattc gataataccg
    10021 cctcttcatg aggcgtgagc gcatcgggca tcagcgaagg cgcgccgata acattttgca
    10081 gcgtgtcgag cagcgtcagg ccagtaaaaa cgcgcatcgt cagttgttgt tctacgacag
    10141 ttaatgtctg ccaggcggga atatcgttcg acagcggcac cttttctggt agccagaaat
    10201 tgctggtcag gcgattccac acctccagat ctttatcgtc agatatcttg ttccagttga
    10261 tggcgctgat acgtgagagt ttcatagata ttccttaaag tgcacaggag acgcagcctt
    10321 caatttcagt gccttccagc gccatctgac gcaggcggat gtaatagagc gttttgatac
    10381 ctttgcgcca ggcgtaaatc tgcgctttgt tgatatcgcg agtggtggcg gtatcgggga
    10441 aaaacagcgt cagcgacagc ccctgatcga catggcgagt cgcttccgcg taggtgtcga
    10501 tgatcttttc tgcgccaatt tcgtaagcgt cctgatacag cgccagattt tcgttagtca
    10561 taaacggggc agggtagtaa acgcgtcctg ttttgccctc tttgcgtatc tctactttcg
    10621 ccacaatcgg atgaatactc gacgtagcat ggttgatata agagatagac ccggttggcg
    10681 gcaccgcctg gagattctgg ttgtatatgc cgtagcgcat cacgtcgtcg cgcagttgcg
    10741 cccacatctc acgggtaggt aacgtaatac cgctgcgggc aaacagttcg ccaactttcg
    10801 ccgttttcgg ctgccagtta ccttgcagat attggctgaa atattcacca ctggcatagc
    10861 gtgactgttt gaacccggcg aaggtttcac cgcgttcgcg cgccagcaac atcgaggtac
    10921 gcagtgcgtg ccaggtgatg gtatagaaat agagattggt gaaatccagg gcttccggcg
    10981 aaccataagc gatgccttcc ctcgccagat aaccatgtaa attcatctgc cccagtccga
    11041 tggcgtgcga ggcggcattt ccggcttcga tggacggcac gctgcggata tgactcatat
    11101 ctgacaccgc cgttaaaccg cgcacggcag tctctaccgt gcgggaaaaa tcgggggaat
    11161 ccatggtgtg cgcaatattc aacgaaccta aattgcagga aatatcatgg cctgtgcggg
    11221 catagtcgag attctcgtca tactctgagg cgctgttaac ctgcaaaatt tctgagcaga
    11281 gattactcat atttatgcgc ccggcgatag ggttagcacg gtttactgtg tcttcataca
    11341 tgatgtaggg atagccggac tcaaactgga tttctgccag tcgctggaag aaatcacggg
    11401 cgttgaggta ttttttgcgg atgcgttcat cggcaaccag ttcgtcatag tgttcgctaa
    11461 tggcgatatc ggcaaacggc ttgccataaa ctcgctcaac gtcatagggc gaaaacagcg
    11521 ccatctgcgc attctctttt gccagatgga aagtgatatc cgggatcacc acgcccagcg
    11581 acagtgtttt aatgcggatt ttttcgtcgg cattttcccg tttcgtgtcg agaaaacgca
    11641 gaatatcggg atgatgagca tgtaaataga ctgcacctgc cccctgacgc gcgccgagtt
    11701 ggttggcata ggaaaatgcg tcttccaaca ttttcatcac cggaattacg ccagaagatt
    11761 gattttcaat acgtttaatt ggcgcgcccg cttcccgcag attcgacagc aaaaatgcta
    11821 cgccgccgcc gcgtttcgac agctgcagtg cggaatttac cgcccgacca atcgactcca
    11881 tattgtcttc aatacgcagc aaaaaacagg aaaccagttc gccgcgctgc tgcttaccgc
    11941 agttgaggaa tgttggcgtg gctggctgaa agcgtcctga cagcatttca tcggtcagtt
    12001 gcaacgccag cgtctcatcg ccttgtgcca gcgtcagcgc caccatcgtg actcgatcgg
    12061 caaaatcttc cagataacgt ttaccgtcga atgtcttcaa cgtatagctg gtgtaaaact
    12121 tccatgcccc gaggaatgtc tggaaacgaa aaccgctggt gtgtgcgtgg gcaaacagcg
    12181 taatgacaaa gtcgcgagag tagcgattaa gaacgctttc atcatagtaa ccttcgttga
    12241 ccagccagtt caggcgctgc tgctggctac tgaaggtcac actgttcgga cgcacatgcg
    12301 tcgcaataaa ggcgtcaacg gcctggcggt ctttatcgaa ctgaatgcga cctgcgctat
    12361 cgtaaagatt aagcatcgca ttcagcgcgt ggtaatccat cgtttcctgc gtcaggcatt
    12421 ctgcggttgt cgttgccaaa attcggttac tcctttacga acgttttcga tatcgctttg
    12481 cgtacccatg agttcaaaac ggtacagcca cggcacgccg catttccggg caatcacatc
    12541 tccggcgcgg ccatacgcct caccaaagtt gcgattacca gaagcaataa cgccgcgaag
    12601 caacgcccgg ttgtgctcgt cgtttaaaaa gcgaattacc tgtcgtggca ccgcgccagc
    12661 cgtaccgccg ccgccgtaag agggcacgat caggatgtaa ggctcgtcta cctgaatccg
    12721 ttcccgctca ttgagcggga tgcgcaccgc gggcagacct aaacgttcga taaaacgctg
    12781 cgtgttttcg gagctgctgg agaagtagac gagctggctc atgcactggc cgcgtgtggc
    12841 gctggatgca gacggttaat catgtccgga cggaaaccag accagctaag atcgccagca
    12901 atcactaccg gcaactgacg aaagccctga gcacgcaacg cttccgctgc ttcaggaacg
    12961 cgatcgacat taatcatttc aaaatcaaag ccccggtttt ccatcgcccg tttggtggcg
    13021 tggcactgaa cgcaatcgtt acgagtataa atagttatgc gcatgattcg tatttctgtt
    13081 taaaatgaag atacggcgcg atgatacgcg tcgggttgtc tctctgttga tacagagata
    13141 ctagatgtag ttgaaaaaag attcaaccac acaatatata gcaaatagtg atcgaaatta
    13201 ccctggatat gagcgtgacg ggggaggggg atttttgtga ttcaccaggc aaaaagaaac
    13261 cccgaagaca ggcttcgggg tcaaagacgc gtatttatta tcatttttgc actacgattt
    13321 gcgcatgctt aacagtgcgc cgataaaaat acctaccgca gctgctgtac ccacgctaca
    13381 ccagggtctt tcacgaacaa aagaatccgc gcagccaacg gcatcgcgcg cggcttgctg
    13441 gacgcgagta cgaccatgca ttcgcgccct ggtttctttc agcaacgcct gagctttact
    13501 gcgtgcggct tcagcttccc ctttggcgtc gctgccccag gacttcaata cagattcgag
    13561 gctgtcagct aattgattga catcattctg gatatcctgc acgccatcat caacgtcgtt
    13621 gcggttcggt cggttaaaca tatgatcccc cattagaatt cgatgtaatt ttcagtgtag
    13681 accataattt tcttaactga ggttcatcgc ggctttatgg acttttctga aagcgttgtg
    13741 aatgcgcaat caggtaaggt aggcccgcgg ttgaaaataa cgaggatcaa atatgtattt
    13801 acgaccagac gaggtggcgc gcgtacttga aaaagtcggt tttactgtcg atgtggtaac
    13861 ccagaaagcg tatggttacc gccgtgggga aaattatgtc tatgttaatc gcgaagcgcg
    13921 aatgggacgt accgctctgg taattcatcc aacattaaaa gaacgcagtt caacactggc
    13981 tgaacccgct tccgatatta aaacttgcga tcattaccag caatttccgc tctatttagc
    14041 aggcgagcga cagcgacacg agcattacgg tatcccgcat ggctttagtt cgcgtgttgc
    14101 gcttgaacgt tatttgaatg gtttatttgg cgaagccagt taaagacgcg actggcgatg
    14161 ccagtcgcga aaagaagagt caggctttta cctgctggta acggctgact ttaaacagtc
    14221 ggcggcaata atcgaggaag tagccataaa ccgcccccat caacatcgaa acaacgatgt
    14281 ttgaactgac cgccgccata atctgatgcc agtctgcgcc caccactaac aagatcgcca
    14341 catacaccgg tgactggaac gtcacataag ccaggatatc agccagattt tttatccagc
    14401 ctttgcgtgc cgcgcgcata aacagatcgc ggtacatacc gtatggccat gcaattaaga
    14461 tgttcaccgg aatcgctacc aatctggaat aaaaagactg ttcgaagctc attccggaga
    14521 ggaaaacttc aatacacatg ttcacgacag aacagtaaac aaccatcgcg aacgtatctg
    14581 caactgcatg acgcaagcgt gactgcggtg agaacatgct aatgctcctt aataaaagcg
    14641 taatgggatg ttaatggaga tgaaagcagt ggatctcatt gccatctgta ttttcgcata
    14701 gcgtatatcg ctggctatga ctaataaatt agtgaaaaat ttttatttag tcagcttttg
    14761 tagataaaat attccgtaac ggctcgtttt ttgtacattg tggattttta catgccgata
    14821 tgatttattt tcaattaaat caattggata tggccttgcc tttaatttca ctaatgaaag
    14881 tttggggggg aggtaacgct ataagcgtag agaattcggt gtttgtcgtg accgggtcat
    14941 ttacagcatt tttgatgcca gaaaatgtac attaacagca gaaagcacca gtgaggggtg
    15001 ttttctttta atctgttata ttgcattttt attcacttcg gttttgtcag gcttacggag
    15061 ttagcgagca gagagcgcca ctctgttcac caataaccta aaatactcat caaaatggct
    15121 aatatataat cttgaaatta ttctgagagt ttcagaaaat aacagctgaa ataatctcgc
    15181 gcaggactgt aaatagatta aattttgtgg aaatataata agtgatcgct tacactacgc
    15241 gacgaaatac ttttttgttt tggcgttaaa aggttttctt tattatgtcc gtaatgttac
    15301 aaagtttaaa taacattcgc accctccgtg cgatggctcg cgaattctcc attgacgttc
    15361 ttgaagaaat gctcgaaaaa ttcagggttg tcactaaaga aagacgtgaa gaagaagaac
    15421 agcagcagcg tgaactggct gagcgtcagg aaaaaattag cacctggctg gagctgatga
    15481 aagctgacgg aattaacccg gaagagttat tgggtaatag ctctgctgct gcaccacgtg
    15541 ctggtaaaaa acgccagccg cgtccggcga aatataaatt cactgatgtt aacggtgaaa
    15601 ctaaaacctg gaccggtcag ggccgtacac cgaagccaat tgctcaggcg ctggcagaag
    15661 gtaaatctct cgacgatttc ctgatctaat tactgaggcc ggatgtcgct gctagggcgc
    15721 gtccggcgtc gcttctcaag ctcgttggtt ccacggtatc ttatctaaca accttgccat
    15781 tccacaaaaa ccgctgattc ctgcaaacag taacccggca ccaacaaagc cacttaataa
    15841 gaagaaaccg ctatttacgg tataacccag tacaacgccg attaatatta aaccacccgc
    15901 agcgatctgc acctggcgca ttaacggcaa gggttgagat ttatttaccg ctactggcaa
    15961 tccagctctt ttccagccat caatcccatc ttcgagtaaa aatatttctg cgggggcggc
    16021 aatcgctgct aatttatcgg cgttattact ggtgcgttta cctgcctggc agtggaatat
    16081 aatttgctcg cgacgtaatt taggtggaag acctgactgt tccagcacgg ataatggagc
    16141 cagatctgct tcaggaatat gttcacgaag atattcatca gcgtcacgaa tatcgattaa
    16201 ctttgcgccg cgtgcgatta attcttgtgc atcatgcggc gaaatggttg tcaaagccat
    16261 agtgactcct tacggacaat agacattttt cagggtggcg ataatggcat ttaccgcctc
    16321 atttttaatg gaatatagaa tgcgttgggc atcccgttgg ctgttgataa gcccttcgtc
    16381 ccgcatacga gcgagatgct gtgatgtcgc agaggcgctc agtccggtaa tgcgcgtcag
    16441 ctctcccgcg ctggtgccgg gggaaccgct aagcatgcac agaatcagca accgtttagg
    16501 gtggctcatt gctttcaata aggccgctgc ctgttcggta ctggcctgta attgcgcgag
    16561 ttcagtcata tttatttaag tattttctaa attaagtaaa ctctaaacta aaaatgcaac
    16621 atataccagc ctcagcagcg taaatgagag taaaagcgta agctgaaact ggcaggctcc
    16681 gctaaaatta ctacgcttaa gagataaaat ctctttttaa acaatgagta attttcttat
    16741 agggagtaca tatgggtttc tggagaatcg tcatcaccat cattctgccg ccgctcggcg
    16801 tgctgctcgg taaagggttc ggttgggcgt tcattattaa tattctgctg acactgcttg
    16861 ggtatatccc cggtttgatt cacgcgttct gggtgcaaac ccgcgattaa acctgtgctc
    16921 acccccgcgg cgttaacgcg ccgcgttcct ctgctacact ttctgagtgt ttccgttaac
    16981 gaatgaggta gctatgggtc tgttcaattt tgtgaaagat gccggagaaa aactctggga
    17041 tgcggttaca ggtcagcacg ataaagacga tcaggcgaag aaggtgcagg agcatctgag
    17101 caaaaccggt atacctgatg ccgataaagt gaacattcaa attgccgacg gcaaagcgac
    17161 ggtcactggt gacggcctga gtcaggaggc gaaggagaaa atccttgttg cggtggggaa
    17221 tatttccggt attgccagtg ttgatgatca ggtgaaaacg gcgacaccag ccactgccag
    17281 ccagttttat accgttaagt ctggcgacac tctgagtgcc atttccaaac aggtctacgg
    17341 taacgctaat ctgtacaata aaatcttcga agcgaataaa ccgatgctaa aaagcccgga
    17401 taaaatttat ccggggcaag tgttgcgtat tccggaagag tagttaattg ccagccatcg
    17461 cctgctggat aatgggaatt ggcgtcagta aatgctggcg cattaactca ctgacgcgcg
    17521 ctgtatctcg cgccagtacc gccgcagtca gggtctggtg ctgatcgtgt ttatcctcca
    17581 gcatttccac cgaaagcacc gttcgccgca gccagataaa tcgataacgc gccgccagat
    17641 caaacaaccg ttcacgcatt tgcagcaaat agtgagaacc acagcccgcc acaattgctg
    17701 tatgaaacgc ctgatgacgc agatcccact catcaagcat tttctcgctg gcgtcacagg
    17761 cttccagctt attgagcaga tgcgcttttg cgagaacgtc tgcctcccat tcatcgccac
    17821 cgcgggcaat cgccagactg accagcatcg cttccatatc gggtggcgtc tatgtcagag
    17881 cagctcctgc tctgacatag acgccacccg ataccctttt tgattcacca ccgtgaccag
    17941 ccgttccgcc accagttgag aaagtgcttc ccgcaacggc ccaacgccaa gtgcataacg
    18001 cgatgtcagt aaactcatcc gtaatttttc atccggttga aaattaccgc gaataatatc
    18061 gttcttcagc cagcgatagc catccagaga cgtaatggtc atggtgtgac tcctgatcat
    18121 tatcagcgcg tattatgaac gggtgttttt tgccacaata ccagcttttt ccagcgagcc
    18181 ataattggca cggtacagat aatccctatc gctaataagc cggtggagat cacttctaac
    18241 tgttgcgccg gacggaatag catcactacc aacacaaagg taataaagcc gatgaccagc
    18301 caggtgagcc agggataaag ccacattcgc aagcgaattt cgcttccttc tgctcgcaga
    18361 attttgcgca tccgcaactg tgaaacggcg atgactaaat aaaccagcag ggcgatagcg
    18421 ccggagctgt cgatcagaaa tttaaatacc ttcgcaggcg cgtaatagtt caccaccacc
    18481 gttaagaatg ctgcgccggt ggagagtaac accgccacgt acggggtttt actgcggttg
    18541 attttaccca ttaccgcggg agcatcaccg cgacggctta aggagtagag catccttgag
    18601 gcggtataca gggccgagtt caggcaactg gttacggaaa gtaatatcac acagtccatg
    18661 attaatttcg catggggaat attgagcaat tccagaaccg agcgataaga tccaacggct
    18721 ttcagccccg gc
//
LOCUS       DADLTI010000073        18719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLTI010000073 DADLTI010000000
VERSION     DADLTI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.073"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN755_002292"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN755_002292"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7381067.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN755_002293"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN755_002293"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7381068.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JN755_002294"
     CDS             complement(3121..3420)
                     /locus_tag="JN755_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381069.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JN755_002295"
     CDS             3595..4590
                     /locus_tag="JN755_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7381070.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JN755_002296"
     CDS             complement(4632..5069)
                     /locus_tag="JN755_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7381071.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JN755_002297"
     CDS             complement(5115..5456)
                     /locus_tag="JN755_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381072.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JN755_002298"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JN755_002298"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7381073.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JN755_002299"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JN755_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JN755_002300"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JN755_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7381074.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JN755_002301"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JN755_002301"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY7381075.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JN755_002302"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JN755_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7381076.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JN755_002303"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JN755_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JN755_002304"
     CDS             complement(13972..14796)
                     /locus_tag="JN755_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7381077.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JN755_002305"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JN755_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JN755_002306"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JN755_002306"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7381078.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
BASE COUNT         4722 a         4741 c         4595 g         4661 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagag agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctagacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggca cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggcc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggga aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgg
//
LOCUS       DADLTI010000074        18507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLTI010000074 DADLTI010000000
VERSION     DADLTI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.074"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..407
                     /locus_tag="JN755_002307"
     CDS             <1..407
                     /locus_tag="JN755_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7381079.1"
                     /translation="PIFARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSS
                     VSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWS
                     FVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            511..2154
                     /gene="eptA"
                     /locus_tag="JN755_002308"
     CDS             511..2154
                     /gene="eptA"
                     /locus_tag="JN755_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7381080.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            2151..2819
                     /gene="basR"
                     /locus_tag="JN755_002309"
     CDS             2151..2819
                     /gene="basR"
                     /locus_tag="JN755_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7381081.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2820..3920
                     /gene="pmrB"
                     /locus_tag="JN755_002310"
     CDS             2820..3920
                     /gene="pmrB"
                     /locus_tag="JN755_002310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7381082.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3896..3985)
                     /gene="pmrR"
                     /locus_tag="JN755_002311"
     CDS             complement(3896..3985)
                     /gene="pmrR"
                     /locus_tag="JN755_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7381083.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(4097..5294)
                     /locus_tag="JN755_002312"
                     /pseudo
     CDS             complement(4097..5294)
                     /locus_tag="JN755_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5558..6436)
                     /locus_tag="JN755_002313"
     CDS             complement(5558..6436)
                     /locus_tag="JN755_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381084.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(6433..8661)
                     /gene="crfC"
                     /locus_tag="JN755_002314"
     CDS             complement(6433..8661)
                     /gene="crfC"
                     /locus_tag="JN755_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7381085.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            9063..9398
                     /locus_tag="JN755_002315"
     CDS             9063..9398
                     /locus_tag="JN755_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7381086.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            9556..9999
                     /gene="yjdN"
                     /locus_tag="JN755_002316"
     CDS             9556..9999
                     /gene="yjdN"
                     /locus_tag="JN755_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7381087.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            10132..10920
                     /gene="phnC"
                     /locus_tag="JN755_002317"
     CDS             10132..10920
                     /gene="phnC"
                     /locus_tag="JN755_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7381088.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            10945..11961
                     /gene="phnD"
                     /locus_tag="JN755_002318"
     CDS             10945..11961
                     /gene="phnD"
                     /locus_tag="JN755_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7381089.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            12067..12846
                     /gene="phnE"
                     /locus_tag="JN755_002319"
     CDS             12067..12846
                     /gene="phnE"
                     /locus_tag="JN755_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY7381090.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            12867..13592
                     /gene="phnF"
                     /locus_tag="JN755_002320"
     CDS             12867..13592
                     /gene="phnF"
                     /locus_tag="JN755_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7381091.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            13593..14045
                     /gene="phnG"
                     /locus_tag="JN755_002321"
     CDS             13593..14045
                     /gene="phnG"
                     /locus_tag="JN755_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7381092.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            14042..14626
                     /gene="phnH"
                     /locus_tag="JN755_002322"
     CDS             14042..14626
                     /gene="phnH"
                     /locus_tag="JN755_002322"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7381093.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            14626..15690
                     /gene="phnI"
                     /locus_tag="JN755_002323"
     CDS             14626..15690
                     /gene="phnI"
                     /locus_tag="JN755_002323"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7381094.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            15683..16528
                     /gene="phnJ"
                     /locus_tag="JN755_002324"
     CDS             15683..16528
                     /gene="phnJ"
                     /locus_tag="JN755_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7381095.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            16525..17283
                     /gene="phnK"
                     /locus_tag="JN755_002325"
     CDS             16525..17283
                     /gene="phnK"
                     /locus_tag="JN755_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY7381096.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            17394..18074
                     /gene="phnL"
                     /locus_tag="JN755_002326"
     CDS             17394..18074
                     /gene="phnL"
                     /locus_tag="JN755_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY7381097.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            18071..>18507
                     /locus_tag="JN755_002327"
     CDS             18071..>18507
                     /locus_tag="JN755_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7381098.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLP"
BASE COUNT         4372 a         5273 c         4889 g         3973 t
ORIGIN      
        1 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
       61 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
      121 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
      181 cgttactgct gctcggccac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
      241 ctaccattgc cttcctctac tgcatttggg ccgtggtggg gtccggggcg aaagaggtta
      301 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
      361 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
      421 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
      481 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
      541 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
      601 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
      661 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
      721 ttcttatggc ttaatcgaac actggcctgc ctgtttattc tggttggcgc ggctgcacaa
      781 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
      841 accactccag cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
      901 agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa tcaaacctgc cacctcgcgt
      961 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     1021 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     1081 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     1141 ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc gttaatgcag
     1201 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     1261 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     1321 tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     1381 tcggatatgc cgcgtgagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg
     1441 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     1501 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tgatcagtgc
     1561 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     1621 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     1681 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     1741 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     1801 attgttgata aagcgattaa tctgcttaaa gaacatcagg ataaatttac caccagcctg
     1861 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     1921 ccttatgcca tcgccccgga tagccaaaaa caggtgccaa tgctgctgtg gctgtcggag
     1981 gattatcaaa agcggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     2041 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     2101 tattaccagg ccgcggatga tattctgcaa acttgcagga gagtgagtaa atgaaaattc
     2161 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     2221 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggacggtc
     2281 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     2341 cccgtatccg gcagaaaaaa tacaccctgc cggtactgat cctcaccgct cgcgatacgc
     2401 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     2461 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     2521 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     2581 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     2641 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     2701 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     2761 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     2821 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     2881 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     2941 ccgagcagat tcaactgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     3001 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     3061 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     3121 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     3181 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     3241 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     3301 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     3361 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     3421 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     3481 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     3541 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     3601 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     3661 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     3721 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     3781 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     3841 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     3901 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     3961 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     4021 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     4081 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     4141 tcgcctgcaa atcggcaatc tcgtggtcaa tattatcgat tttctgctcg atattatcgt
     4201 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     4261 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     4321 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     4381 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     4441 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccataaagc
     4501 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     4561 taatcagaat aaacgccggg atcgccctgt tccagtttat caacatgctg ctggaacgcc
     4621 ggagtttctt ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata
     4681 aagaacggaa tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt
     4741 aacaccacca cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg
     4801 cccataaagc cacgtttacg gtcgggggag tattccgcaa caaatatcga cgccccggta
     4861 tattcaccac cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc
     4921 caaataccaa tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata
     4981 atcacaatag tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag
     5041 aagagtccgc caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc
     5101 atctgcacgc tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa
     5161 ccataaacac caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct
     5221 ttacgcagtt taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt
     5281 ttccttttca gcatagcttt cctcgcagag ttgacccatt tccgcaaacg ccagaaaaat
     5341 cagcgcgacg gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt
     5401 agcgggtttg cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa
     5461 ttgggcagtt aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca
     5521 tggatttcgt ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga
     5581 tttcctcgac cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg
     5641 cgacagtttg ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc
     5701 actttcccag ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg
     5761 caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc tctgccagat
     5821 gttgtgtcgc tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca
     5881 gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc
     5941 caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct
     6001 gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac
     6061 gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt
     6121 gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca
     6181 gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc agactaagcg
     6241 gcgcatttaa catgccgctg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct
     6301 gcatcagtcg ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct
     6361 gcgccagatc cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca
     6421 gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag
     6481 ctgagcgtcg gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat
     6541 cagcctgacc accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg
     6601 taattctgtc aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga
     6661 ttcatcgacc tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc
     6721 gcttaatgct cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc
     6781 ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg
     6841 cgtgctctgc tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt
     6901 gttgaagtgg ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga
     6961 ctcgctcaat tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg
     7021 gcaaagggtg gattccagct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg
     7081 cgccgtctgt aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc
     7141 ctgaaacaag gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt
     7201 ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt
     7261 gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca
     7321 agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt
     7381 caacagttta tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg
     7441 aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg
     7501 acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg
     7561 ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa
     7621 ctcatagcgc gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg
     7681 tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc
     7741 gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg
     7801 ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata
     7861 atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag
     7921 atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata
     7981 actttccagc cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc
     8041 ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt
     8101 caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac
     8161 gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt
     8221 cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat
     8281 tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag
     8341 cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc
     8401 attaatggtg gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag
     8461 tcgggaaatt ttacgcaact cattattcag catcgcgtga cgttcggcga tattaggctg
     8521 actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc
     8581 cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc
     8641 atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt
     8701 tagccagatt gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt
     8761 atatttagtg tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc
     8821 agaaagataa aaatatcatg taaataaaaa aaagaacaag tagagcatta acattatctt
     8881 aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg
     8941 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
     9001 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
     9061 acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca
     9121 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
     9181 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca
     9241 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
     9301 aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc
     9361 cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg
     9421 aataaggcgc tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg
     9481 tcttatcagg cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta
     9541 actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac
     9601 gcgattgcct attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc
     9661 gaaatgccga aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc
     9721 gataccgcca tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat
     9781 gctataccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag
     9841 gtcgaagaag gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc
     9901 tggcaggaaa ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg
     9961 tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc
    10021 ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa
    10081 tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg
    10141 attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt
    10201 gatctgaaca ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa
    10261 tccacccttt tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat
    10321 atcgagctac tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa
    10381 agccgcgcca acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta
    10441 ctggagaacg tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc
    10501 tggttcaccg gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg
    10561 cattttgccc atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt
    10621 gcccgtgcgc tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg
    10681 gacccggaat cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc
    10741 atcaccgtgg tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc
    10801 gtcgccctgc gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa
    10861 cgttttgacc atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga
    10921 tatccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc
    10981 agcatgttca gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg
    11041 ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg
    11101 tttttacagg atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac
    11161 tacgcgggca ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac
    11221 ctgtcggcga tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg
    11281 gcggatggat cgtcgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac
    11341 aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac
    11401 tccacctctg gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc
    11461 agcgacttca agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac
    11521 aagcaggtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg
    11581 ccggagaagc ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg
    11641 atcgtctggc gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg
    11701 aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctaggctg ggcgccgttc
    11761 cgcgcttcca gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata
    11821 caaagcgtga aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag
    11881 attcaggcgc agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg
    11941 gtgagtaaag cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac
    12001 aatgtgcagg cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag
    12061 ccaaacatgc aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc
    12121 tgggccgttg ttctcgccgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt
    12181 acgctgatta aagacggcgg caacatggca accttcgctg ccgacttctt cccgcccgat
    12241 ttcagccagt ggcaggatta cctcaccgaa atggccgtca cgctgcaaat cgccgtctgg
    12301 ggcaccgcgc tggcagtggt tctctccatc cccttcggtc tgatgagcgc cgaaaacttg
    12361 gtgccgtggt gggtttacca gcccgttcgc cgcctgatgg acgcctgccg cgccattaac
    12421 gaaatggtct tcgccatgct gttcgtggtc gccgtcggcc tcggcccgtt cgctggcgtg
    12481 ctggcgctgt ttatccacac caccggcgtg ctctccaagc tgctttccga agcggaggaa
    12541 gcgatcgaac ccggcccggt ggaaggcatt cgcgccaccg gcgccaacaa actcgaagag
    12601 atcctttacg gcgtgctgcc gcaggtgatg ccgctgctga tctcctactc cctctatcgc
    12661 ttcgaatcca acgtccgctc ggcgaccgtc gtcggcatgg tcggcgcggg cgggatcggc
    12721 gtcaccctgt gggaagcgat tcgcggtttc cagttccagc aaacctgcgc cctgatggtg
    12781 cttatcatcg tcacggtcag cctgctggat ttcctctctc aacggttgcg taagcacttt
    12841 atctgataag cgaggcattg atatctatgc acttgtctac acatccgacc agctacccaa
    12901 cacgctatca ggagatagcc gcaaaacttg agcaggagct tcgtcaacac taccgctgcg
    12961 gcgactatct tcccgccgag cagcaactgg cggcgcgctt tgaggtgaac cgccacaccc
    13021 tgcgccgcgc catcgaccaa ctggtggaaa aaggctgggt acagcgccgt cagggcgtcg
    13081 gcgtgctggt gctgatgcgc ccgttcgatt acccgctcaa cgcccaggcg cgttttagcc
    13141 agaacctgct ggaccagggc agccatccca ccagcgaaaa actgctgtcg gtattgcgcc
    13201 ccgcgtccgg tcacgttgct gacgcgctgg ggattaccga gggcgagaac gttatccatt
    13261 tgcgcaccct gcgccgggtc aacggcgtcg cgctctgttt aatcgaccac tactttgcgg
    13321 acctcaccct ttggccgacg ctgcaacgct tcgacagcgg ttcgctgcac gattttctgc
    13381 gcgagcaaac cggaattgcg ctgcgccgca gccagacgcg gatcagcgcc cgccgcgccc
    13441 aggccaaaga gtgccagcgc cttgaaatcc cgaatatgtc gccgctgctg tgcgtgcgca
    13501 cccttaacca ccgtgacggt gaaagcagcc cggcggagta ctccgtcagc ctgacgcgcg
    13561 ccgacatgat tgaattcact atggagcact gaatgcacgc agataccgcg acccgccagc
    13621 actggatgtc cgtactggcg cacagccaac cggctgaact ggcagcccgc ctgaacgcgc
    13681 tgaacatcac cgccgactat gaagtgatcc gcaccgccga aaccggcctg gtacagattc
    13741 aggcgcgaat gggcggcacc ggcgaacgct tttttgccgg cgacgccacg ctgacccgcg
    13801 ccgccgtacg cctgactgac ggcaccctcg gctacagctg ggtgctgggg cgtgataaac
    13861 agcacaccga acgctgcgcg ctgattgatg cgctgatgca gcaatctcgc cactttcaaa
    13921 acttatcaga aacccttatt gccccgctgg acgctgaccg tatggcacgc attgccgcac
    13981 gccaggccga agtgaacgcc agccgggtcg acttctttac gatgattcgc ggagacaacg
    14041 catgaccctg gaaaccgctt ttatgcttcc cgtgcaggat gcccagcaca gttttcgtcg
    14101 cctgttaaag gccataagcg agccgggcgt gattgtcgcc ctgcatcagc tcaagcgcgg
    14161 ctggcagccg ctgaatatcg ccaccaccag cgtgctgctg acgctggctg ataacgacac
    14221 gccggtgtgg cttgccgcgc cattgagtaa cgatatcgtc aatcagagcc tgcgttttca
    14281 taccaacgcg ccgctggtca gccagccgga acaggcgacc ttcgcggtga cggatgacgg
    14341 aatttccagc aaacagctca acgccctttc caccggcacc gccgttgcgc cggaagcggg
    14401 cgcgacgctg attttacagg tcgccagcct gagcggcggg cgcatgttgc gtctcaccgg
    14461 cgcgggtatt gccgaagaac gaatgatcgc tccgcagctg ccggagtgca ttctgcacga
    14521 actcaccgag cgcccgcacc cgttcccgct cggcatcgac ctgatcctga cctgcggcga
    14581 acgcctgctg gctattccgc gaaccactca tgtggaggtg tgctgatgta cgttgccgtg
    14641 aaagggggcg aaaaggcgat cgacgccgcc cacgccctgc aagagagccg acgccggggc
    14701 gataccgatt tgcccgaact gagcgtcgcc caaattgaac agcagcttaa tctcgcggtg
    14761 gatcgcgtga tgaccgaagg cggcgttgcc gaccgcgaac tggcggcgct ggcgctgaaa
    14821 caggccagcg gcgataacgt tgaagcaatt ttcctgctgc gcgcctaccg caccacgctg
    14881 gcgaaactgg cggtaagcga gccgctcgac accaccgaga tgcgtctcga acggcgtatt
    14941 tcggcggttt ataaagacat tcccggcggc cagctgcttg gcccaaccta cgactacacc
    15001 catcgcctgc tcgatttcac cctgctggca aacggtgaag cgccgacgct gaccaccgcc
    15061 gacagcgaac aacagccgtc gccgcacgtt ttcagcctgc tggcgcgtca ggggctggcg
    15121 aagtttgaag aagatagcgg cgcacagccg gatgacatca cccgcacgcc gccggtttac
    15181 ccctgctcac gctcctcccg cctgcaacaa ttaatgcgcg gggacgaagg ctatttgctg
    15241 gcgctggcct actccaccca gcgtggttac ggacgcaatc acccgttcgc gggcgagatc
    15301 cgcagtggtt acatcgacgt gtcgattgtg ccggaagagc tgggatttgc ggtaaacgtc
    15361 ggcgaactgc tgatgaccga gtgtgaaatg gtcaacggtt ttatcgaccc gccggatgag
    15421 ccgccgcact tcacgcgcgg ctacgggctg gtattcggca tgagcgagcg caaagcgatg
    15481 gcaatggcgc tggttgaccg tgcgttgcaa gccccggaat acggcgagca cgcgacaggc
    15541 ccggcgcagg atgaagagtt cgtgctggcg catgccgaca acgtcgaagc cgcaggcttt
    15601 gtctcgcacc tcaaactccc ccactacgtc gatttccaag ccgaactgga gctactcaaa
    15661 cgtctgcaac aggagcagaa ccatggctaa tctgagcggc tacaactttg cctacctcga
    15721 cgagcagacc aaacgcatga tccgccgcgc catcttaaaa gcggtggcta tccccggtta
    15781 tcaggtgccg tttggcgggc gcgagatgcc gatgccctac ggctggggaa ccggcggcat
    15841 tcagctcacc gccagcgtga ttggcgaaag cgacgtgttg aaggtgattg accagggcgc
    15901 agacgacacc actaacgccg tgtcgattcg caacttcttc aagcgcgtga ccggggtgaa
    15961 caccactgaa cgtacggacg atgcgacgct tatccagacg cgccatcgca tccccgaaac
    16021 gccgctgacc gaagatcaga tagttatttt ccaggtgccg atccccgaac cgctgcgctt
    16081 tatcgagccg cgcgaaacgg aaacccgcac tatgcacgcg ctggaggagt acggcgtgat
    16141 gcaggtgaaa ctgtatgaag atatcgcccg cttcggtcat atcgccacca cctatgctta
    16201 tccggtgaag gtgaacgggc gctacgtgat ggacccgtcg ccgatcccga aattcgataa
    16261 cccgaaaatg gacatgatgc ccgccctgca gctgttcggc gcagggcgcg agaagcgcat
    16321 ctatgcggtg ccgccgttta cccgcgtgga aagtctcgat ttcgacgatc acccgttcac
    16381 cgttcagcag tgggatgagc catgcgccat ctgcggatcg acccacagct atctcgacga
    16441 agtggtgctg gatgacgccg gaaaccgcat gtttgtctgc tccgataccg attattgccg
    16501 ccaacagagc gaggcaaaaa accaatgaat caaccgttac tttcggtcaa taacctgacc
    16561 cacctttacg cgccgggcaa aggctttagc gatgtctctt ttgatttatg gccgggggaa
    16621 gtgctgggca tcgtcgggga atccggctcc gggaagacca cgctgctgaa atcaatctcc
    16681 gcgcgcctga cgccgcagca gggggagatt cgctacgaga atcgttcgtt gtatgcgatg
    16741 agcgaggccg accgccgtcg cctgctgcgc accgaatggg gcgtggtgca tcagcatcca
    16801 ctcgacggcc tgcgtcgcca ggtctcggca ggcggcaata tcggcgagcg gctgatggcg
    16861 accggggccc gtcattacgg cgacatccgc gccaccgcgc agaagtggct ggaagaggtg
    16921 gagattcccg ccaaccggat cgacgacctg ccgaccacct tttccggcgg catgcagcaa
    16981 cgtttgcaga ttgcccgcaa cctggtgacg catccgaagc tggtgtttat ggacgagcca
    17041 accggtgggc tggatgtgtc ggtgcaggca cgcctgctcg atctactgcg cggcctggtg
    17101 gtggagctga acctggcggt ggtgattgtc acccatgatt taggcgtcgc ccgcctgcta
    17161 gcggaccgtt tgctggtgat gaagcagggg caagtagtgg agagtgggtt aaccgaccgc
    17221 gtgctcgacg acccgcacca tccgtatacc cagctgctgg tgtcatcggt tttgcagaat
    17281 tgagccggtg ccggatgcgg cgtaaacgcc ttatctggcc tacaaatgcg ctccccgtag
    17341 gtcggataag acgcatcagc gtcgcatccg acacccgaac cacgaggcga aaaatgatta
    17401 acgtacaaaa cgtcagtaaa accttcatcc tgcaccagca aaacggcgtg cgcctgcccg
    17461 tcctcaaccg cgcctcgctc accgtcaacg cgggcgaatg cgtggtgctc cacggccact
    17521 ccggcagcgg caaatcaacc ctgctgcgct cgctgtacgc caactatctg cccgatgaag
    17581 ggcaaatcca gatcaaacac ggtgacgagt gggtagacct ggtcaccgcg ccagcgcgca
    17641 aagtggtgga gatccgcaaa accaccgtcg gctgggtgag ccagtttctg cgcgtcatcc
    17701 cgcgtatttc agcactggaa gtggtgatgc agccgctgct cgataccggc gtaccgcgtg
    17761 aagcctgcgc cgctaaagcc gcgcgtctgc tcacccgcct gaacgtgccg gaacgcctgt
    17821 ggcacctggc gccatcaacc ttctccggcg gcgaacagca gcgcgtcaac atcgcccgcg
    17881 gctttatcgt cgactacccg attctgctgc tcgacgaacc taccgcctcg ctggacgcca
    17941 aaaacagcgc cgcggtggtg gaactgatcc gcgaagccaa agctcgtggc gcagccattg
    18001 tcggcatctt ccatgacgaa gccgtacgta atgacgttgc cgaccgcctg cacccaatgg
    18061 aagcctcttc atgattatca ataacgtcaa attggtgctg gaaaacgaag tggtacacgg
    18121 ttcactggag gtgcaggacg gcgaaatccg cgcctttgcc gaaagccaga gccgcctgcc
    18181 ggaggcgatg gacggcgaag gcggctggct gctgccgggg ctgattgagc tgcataccga
    18241 taatctggat aaattcttca ccccgcgccc gaaagtcgac tggcccgccc attcggcgat
    18301 gagcagccac gacgcgctga tggtggcaag cggcatcacc accgtactgg acgccgtggc
    18361 gattggtgat gtgcgcgacg gcggcgatcg gttggagaat ctggagaaga tgatcaacgc
    18421 catcgaagag acgcagaaac gcggcgtcaa ccgcgccgag caccgtctgc tcctgcgctg
    18481 cgaactgccg catcacacca cgctgcc
//
LOCUS       DADLTI010000075        18265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLTI010000075 DADLTI010000000
VERSION     DADLTI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.075"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(66..824)
                     /gene="aroD"
                     /locus_tag="JN755_002328"
     CDS             complement(66..824)
                     /gene="aroD"
                     /locus_tag="JN755_002328"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7381099.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="JN755_002329"
     CDS             complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="JN755_002329"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7381100.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1733..2947)
                     /locus_tag="JN755_002330"
     CDS             complement(1733..2947)
                     /locus_tag="JN755_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381101.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3187..4332)
                     /locus_tag="JN755_002331"
     CDS             complement(3187..4332)
                     /locus_tag="JN755_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381102.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            complement(4491..4847)
                     /locus_tag="JN755_002332"
                     /pseudo
     CDS             complement(4491..4847)
                     /locus_tag="JN755_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4983..5090)
                     /gene="rprA"
                     /locus_tag="JN755_002333"
     ncRNA           complement(4983..5090)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN755_002333"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5276..6388)
                     /gene="ydiK"
                     /locus_tag="JN755_002334"
     CDS             complement(5276..6388)
                     /gene="ydiK"
                     /locus_tag="JN755_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7381103.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6777..9833
                     /locus_tag="JN755_002335"
     CDS             6777..9833
                     /locus_tag="JN755_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7381104.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9830..10240
                     /gene="menI"
                     /locus_tag="JN755_002336"
     CDS             9830..10240
                     /gene="menI"
                     /locus_tag="JN755_002336"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7381105.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10340..10528
                     /locus_tag="JN755_002337"
     CDS             10340..10528
                     /locus_tag="JN755_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381106.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11076..11444
                     /gene="sufA"
                     /locus_tag="JN755_002338"
     CDS             11076..11444
                     /gene="sufA"
                     /locus_tag="JN755_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7381107.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11453..12940
                     /gene="sufB"
                     /locus_tag="JN755_002339"
     CDS             11453..12940
                     /gene="sufB"
                     /locus_tag="JN755_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7381108.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12950..13696
                     /gene="sufC"
                     /locus_tag="JN755_002340"
     CDS             12950..13696
                     /gene="sufC"
                     /locus_tag="JN755_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7381109.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13671..14942
                     /gene="sufD"
                     /locus_tag="JN755_002341"
     CDS             13671..14942
                     /gene="sufD"
                     /locus_tag="JN755_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7381110.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14939..16159
                     /gene="sufS"
                     /locus_tag="JN755_002342"
     CDS             14939..16159
                     /gene="sufS"
                     /locus_tag="JN755_002342"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7381111.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16172..16588
                     /gene="sufE"
                     /locus_tag="JN755_002343"
     CDS             16172..16588
                     /gene="sufE"
                     /locus_tag="JN755_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7381112.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16737..17741
                     /gene="ldtE"
                     /locus_tag="JN755_002344"
     CDS             16737..17741
                     /gene="ldtE"
                     /locus_tag="JN755_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7381113.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17805..18041)
                     /locus_tag="JN755_002345"
     CDS             complement(17805..18041)
                     /locus_tag="JN755_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7381114.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4811 a         4403 c         4689 g         4362 t
ORIGIN      
        1 aaatacggtt aaaaatactt tttttatttc ggcgggggag ggtatttccg ccgaaatatt
       61 attgcttatg cctgatgtaa aatagttaat accgtgcgca aatcatttac cgagatttgc
      121 cctggcgcag aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca
      181 gccagacgag aaattacgcc agtttttgcc atcgacatcg tgatgattgg acgatcggca
      241 tactgctctt gcatctccag ggtcgcggca agcaacgtca gcacatcgct ggtactttgc
      301 ggcatcagcg caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca
      361 atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca
      421 tcatgcgcgt gggcgtaggc gacggtttct ttgacctgat catcgccggt aaataactcc
      481 agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc
      541 tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt
      601 ttttctggca tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag
      661 aggtcggcaa agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg
      721 agagcttcgg atttcacgcg ggcgatatct ttcgccatca gcgagacgat gattttaggt
      781 gcgcccgcac caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc
      841 acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa
      901 tctttgccag tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca
      961 tcaatcgttt tgcaaccagc ttgttgtgcc tgctgcaata acttcgtcat atgcgggtta
     1021 tacacgcatt cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat
     1081 tcattctcaa ggggtttcat acctactttt gtgccattgg ttaaaatgtc ggcggaagcc
     1141 agggcttcag caaaggcttg ctgatcggcg agatcggtaa ccgtgacgac acaatcggtg
     1201 ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc ccgacggtta
     1261 aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca
     1321 cctccggccc ctaacagcac catcgttttg cctttgatat caaaaccgct ctctttaatg
     1381 gcgcgaatat ggcccgtgcc gtcggtgtta tagccacgca gatagccatc atcattaacg
     1441 atggtgttga tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac
     1501 gccagttgtt tgttcggcat cgacacacca gttccgcgca ttttgagggc ttttaatcct
     1561 tcaattgctc cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat
     1621 cccgcttttt ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa
     1681 gccatcaatc caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc
     1741 tatgcttaat tgtgtttaac cgggtgctaa atttgcgttc gccaaaccgc acatcattct
     1801 caggaataat aaaaacgcgg taatagcggc taaaaacaat aattgcggta atcagggcca
     1861 gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac
     1921 cggtaatcag cggaataaca aagttagcca gtccacccat catcatataa atactggtga
     1981 ctttggcttt gcttttgggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc
     2041 cgccagctgc tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt
     2101 acaggtagat aatggcagct gttattgttg ccagcgcaga gttaaataca ttagcccaga
     2161 tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagacc aacgagccca
     2221 tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg
     2281 catatttggg catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca
     2341 gtaccgatga aacaccttcc agccagacta acggtttgct gttcatttgc ggtaattcat
     2401 tagctacgct ggcgtccacc aactggctgg ggaatttgct tttcaacagc atcagcgtga
     2461 tcagtacaaa cagaataccc ggaataatca gcccatagcc gtaccagata ttattgagca
     2521 acatatagct caccagcatt gggtagaaca tttgcccaaa tgacaccatc gctttaacca
     2581 gtatgaccgc cgaaccagag gctttcggaa agcattccat gagcgcgggg tagccacccg
     2641 tatccagcgc tgagttagcg atacctacgc acactgccag accgtaggcg agagttaaat
     2701 tcgggcaagc gggaatacca aagaagaata gcagatacat tattactgcc attaatatca
     2761 ccgcccgacg accaaactta tcggagatca caccgaagaa taaaatactg atcaatcgcc
     2821 ccaaaccgat accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa aacttttctg
     2881 ccagagatgt catattttgg gcaagcgtaa taacactaat gccgtgcagg aaatagctga
     2941 agtaaataca aagaacagcc aggataaatg gcgtgctgaa agataatttc gttaccgtac
     3001 gtattgttct ccgacggttt acataattac tatactgaag atgattttcc atgggtacat
     3061 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
     3121 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
     3181 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg
     3241 gctgcgtaag ccgataaaca gtgccagtaa aaaaccgatg gcagtatcga accacataat
     3301 atcggcaata cttctttggg acagatgagc cgtaatcaac ggaatagtaa aggtcgcaat
     3361 actgcccgca ctgtaataga tccctgtagc tttaccttta gcgtaaggga aacgttcagc
     3421 tattaacgtc aggccaattt gcacaacacc tccagcagag gtaaaaccaa tgacaaaagc
     3481 aaatattatc accacataaa atgtgggatg caggcagacg gtaaacagag caataaatga
     3541 gataaaggtg tacagcatca gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc
     3601 ggtaataaat acacaaagca gcgaacccac ggtgtagata ctgagtagtt tgatcgacat
     3661 agtgtatgac atgcctgcaa caaattgtcc gtactgtgcc agccactggc taaccagata
     3721 aaacgttgcc attgagatat agccatataa ggtataactg gctaaatcga taattgaaca
     3781 gcgatgttcc gtagagctgg tggttttctt tatgacaggt aagcgacgac ccggatgggg
     3841 tgggaacgta caacggtata aaaacagagc gttaataaac ataatgcctg cagcaatcat
     3901 aaaggaccaa ccgaaccaca gttcagccca cactaacagg ctaatgatta gcggtaataa
     3961 aaattgtccg ctggaaacaa atgctttaat taaaatattg gctgtcccag gtgagcgtgg
     4021 aaaagcttcc atcaaactgg gataagtgcc tgcatcgaga aaactgtttg ccattcccgc
     4081 cagaaagcca aaaacataag cgataatgat gttattggtc tgcaggatgc caaaaaagaa
     4141 ggccatatag cagcacattc cgagcatgat aaaagggcgg cgaccaaagc gatcggataa
     4201 taatcctgca aaaagcaaga cacttaatcg accaatgccc agcgatgaga taactatcga
     4261 gacacccgcg gcattagtct gacaaagtgt ctccagcgag gccatattca ggctcatcaa
     4321 aatgacgccc ataccatgca ccaggtaata aaatacaacc caagtgcggt agggtaatag
     4381 ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca
     4441 gtatccattc attattgttg taacaacgac aattcctttc tgtggaaaaa ttaacaaagc
     4501 cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt ccagtcaata
     4561 aaattgccat cagggtagac ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg
     4621 ttgttgccga tgcggttatt tattttctct atgatggcat gaagatgttt ttttcgttac
     4681 tgacgcatag ccaacagttg ctcgactaca caatcaggaa tggcgcattt gccattttcc
     4741 cactgacgcc agctttcact gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata
     4801 gtcatggcaa aaatatggcg gagtgcctgg agttcataag cgttcatcgt gtaatggctg
     4861 catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct tgaagagagt
     4921 cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga agcggaaaaa
     4981 tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa ttcgttgttt
     5041 cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt gagctaatag
     5101 taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga
     5161 atttgcgaca ggtactacca gctgaatggc ggtaagcagg aaaatatggg gaagagcaag
     5221 caaatatcaa ttagcggtga ttaacaatca ggcggcagag ccgcctgatg agaaattact
     5281 tattcggttt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg tccgtcggcg
     5341 gcgggacttc ttccacccac gcggcaaaca gacgccagga aacggctaac agaaccggac
     5401 caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacgcca gagaggatca
     5461 ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg ttatccagcg
     5521 tgccaaccac accgctccac actaacaata ccgttcccca ggtggtatcg ccggtccagt
     5581 agagccagat aatcgccgga atcagtaccg gcaacgggcc aagctggaca aggcaggaga
     5641 ggatcattaa caccgttagc agcgttgcat aaggtacgcc ggatacggcg aggccgatac
     5701 cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc gcgaggatag
     5761 cctgtgccgc cagcagcacg gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat
     5821 ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcactg aagagaagca
     5881 tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg aaccaagtgg
     5941 tggtggtgcc aatataaggg cggactttcg ccatgatcgc cgtacccccc atatccagca
     6001 agttgtgcca gcctgcatac agcttcgcgc caatcaccgg aatggtatta agccacgcca
     6061 gatcgggtaa cgtcatgtcg ccgctggaaa tggctttaat tagcgggccg ctgccgtcga
     6121 cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat aacagcgtca
     6181 tcaccagaac ggcgagagag cggcggccaa acatgatctt ttgcaaacgt aacaataccg
     6241 gccaggtggc gataaccacc gtaccggccc atgcaaagcc gagaataaag ggttgaacaa
     6301 tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga atttgtgcga
     6361 catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt gcgtcaaatc
     6421 gtcgacgcga cgtggacacg cgaaactcac ctgcataatg atgagcaatt tcagcggttt
     6481 ttaacaggcc gattctgtcc ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac
     6541 aactcaggca acacgcaaac gatttactcg tcgtatttca agttgcatgt gctgcggcta
     6601 cgttcgttca ccccagtcac ttactattgt aagctcctgg agttcactcg cttgccgcct
     6661 tcctgcaact cgaaatccat agagtaaata aacatcacct ttcagtgcgc tattgcagcc
     6721 gcctgaaaga atagtgtatc gacctacatc acagaccgca ggaaagggtc aatataatga
     6781 ttccacagat ttcccaggca cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc
     6841 tggagcaaca aggttttacc ggcgatgcgg cgacaagtta tgccgatcgt ctgacaatgt
     6901 cgaccgacaa cagtatttac caacttctcc ccgatgcggt ggtatttccg cgttcaaccg
     6961 cagatgtggt gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta
     7021 ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct caaccagggg attattgttg
     7081 atatgtcccg ccatatgaac cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg
     7141 ttgaggccgg ggtgataaaa gaccaactca atcagtacct gaaaccgttc ggctactttt
     7201 ttgcgccgga actttcgacc agcaaccggg caacgctcgg tgggatgatc aataccgatg
     7261 catccggtca gggatcgctg gtctatggca aaacgtcaga tcacgtactt ggcgtacgcg
     7321 cggtgttgtt ggggggcgat attctcgata cgcaaccttt acccgtcgag ctggcggaaa
     7381 cgctgggtaa atccaatacc acaatcgggc gaatttataa cacggtttat caacgttgcc
     7441 gtcagcaacg ccagttaatc atcgacaact tccccaaact taaccgcttt cttaccggtt
     7501 acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg cgcattctga
     7561 cgggttcaga agggacgctg gcctttatta ccgaagcgcg gctggatatt acgcgcttgc
     7621 ctaaagtgcg ccgtctggtg aatgtcaaat atgactcttt tgactctgcg ctgcgtaacg
     7681 cgccgtttat ggttgaggcg cgggcgcttt cggtagagac ggtggactca aaagtgctga
     7741 atctggcgcg ggaagatatt gtctggcatt ccgtcagcga gttgattacc gatgtgcctg
     7801 acaaagagat gctcggactg aacattgtgg aatttgctgg tgatgatgag gcgctgattg
     7861 acgagcgagt taatgcactc tgtgtgcggc ttgatgagct gattgctagc caacaggcag
     7921 gtgtgattgg ctggcaggtg tgccgcgagc tggcgggggt tgaacgtatc tatgcgatgc
     7981 gcaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca attccgtttg
     8041 ctgaggatac ctgcgtaccg ccggaacatc tggccgatta tattgctgaa tttcgcgcgc
     8101 tgctcgacag ccacggctta agctacggta tgttcggtca cgtcgacgca ggtgtcttgc
     8161 acgtccgtcc ggcactggat atgtgcgatc cgcaacaaga gattttgatg aagcaaatct
     8221 ctgatgacgt ggtggcgctg actgcgaaat acggtggttt gttgtggggc gagcacggca
     8281 aaggttttcg cgctgaatac agcccggcgt ttttcggtga ggaacttttt gcagaactgc
     8341 gcaaagtgaa agcggcattt gacccgcata accgactcaa cccagggaag atttgcccgc
     8401 cagaaggtct cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt acattcgatc
     8461 ggcagatccc cattgcggta cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg
     8521 gtttatgctt caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga
     8581 accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg cgcttgttgg
     8641 cggatcgcgg cgttgaccca ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt
     8701 tgcggacgtt aattgcccgc acgcgcaata gctggcatgc gaataaaggc gaatatgact
     8761 tctcacacga agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagct tgttcgaccc
     8821 agtgccccat caaaattgat gtgccggagt ttcgctctcg ttttctgcag ctctatcaca
     8881 cccgttattt acgcccgctt cgcgaccacc tcgttgctac ggtcgagagc tacgcaccgc
     8941 tgatggcacg cgcgccgaaa acctttaact tcttcattaa ccagccgctg gtgcgcaaac
     9001 tctcggaaaa acatatcggc atggttgatt tgccactgct gtcggtcccc tcgctacaac
     9061 aacaaatggt ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa gctctcaatg
     9121 cagagcagaa agcgcgcacg gtgttggtgg tgcaggaccc ctttaccagc tattacgatg
     9181 cgcaagtggt ggcggatttt gtccgtctgg ttgaaaaatt aggtttccag cctgtgttac
     9241 tgccattttc gccaaatggc aaagcccagc atatcaaagg ctttcttaat cgttttgcga
     9301 agacggcgaa aaagacggcg gatttcctca accgtatggc taagcttggg atgccaatgg
     9361 tgggcgtcga tccggcgctg gtactttgtt atcgcgatga atataaactg gcgctgggcg
     9421 aggaacgtgg cgagtttaac gtcttacttg cgaatgaatg gctggcaagc gcacttgagt
     9481 cacatccagt ggctacagtc agcggtgaat catggtattt ctttggtcac tgtaccgagg
     9541 ttaccgcttt gccgggcgcg ccagcacaat gggccgcgat atttgcccgt tttggcgcga
     9601 aactggaaaa tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga catgaagcga
     9661 aaaaccatga aaattcgctc gggatctatg agttatcctg gcatcaggct atgcagcgac
     9721 tgccgcgtaa ccgctgtctg gcgaccggat attcctgccg tagccaggta aaacgggttg
     9781 aaggcacggg ggtacgccat cctgtgcagg ctttactgga gattattaaa tgatatggaa
     9841 acggaaaatc accctggaag cactgaatgc tatgggtgaa ggaaacatgg tggggttcct
     9901 ggatattcgc tttgaacata ttggtgatga cacccttgaa gcgacaatgc cagtagactc
     9961 gcggacaaag cagcctttcg ggttgctgca tggaggagca tccgtggtac tggccgaaag
    10021 tatcggttcc gttgccggtt atttatgtac cgaaggtgag caaaaagtgg ttggtctgga
    10081 aatcaatgct aatcacgtcc gctcggcacg agaagggcgg gtgcgcggca tatgtaaacc
    10141 gttgcatctc ggttcgcgtc accaggtctg gcagattgaa atcttcgatg agaaagggcg
    10201 tttgtgctgt tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt
    10261 gacgcatatt gcttttggtc attatctgca cagtaaacgg tggtatactg tgcaggtctt
    10321 gtctaaaagc gaggatgcta tgtctactca acttgatccc acccagctgg ccattgaatt
    10381 tttacgccgt gaccagagca atctttcacc tgcccaatat ctcaaacgtc tgaaacaact
    10441 agaacttgaa tttgctgacc tgcttactct ctcttctgct gaattaaaag aagaaatcta
    10501 ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg
    10561 acctggatta acctccgtcc aggttcgatg accttcttaa tactgcttat ctaccgcctg
    10621 atattgttca aaaataatac taatcgcagt ttgtgttaaa acggcgggtt agctttatga
    10681 gattattctt atcgcccctt caagagctaa gccactgaga gtgccggaga taagcgccgg
    10741 atggggtaga aacccttaag cctgtgtcgc acagacttaa gggttttctt atttctgaac
    10801 gctgcacttt tttacctatc tcttaaatag tcattttcaa tacaactttt tctttttcat
    10861 cacagcctta atgctcctta gataataacc attatctaac aatgagatac ctaattctta
    10921 gcgtgcctgt taacccacac atcaggggct atgcttatta aaagcaggca aaaagcatgt
    10981 tttacggtaa agcccctgcg tttgctgggt tgaactgata atcattatca ctaacatgct
    11041 gttatacgct gaaagcgatg aagtgaggta aatagatgga catgcattca ggaaccttta
    11101 acccacagga tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg atacacatcc
    11161 gcgagctggt ggcaaagcag ccaggtatgg tcggcgtgcg cttaggtgtg aagcaaacgg
    11221 gctgcgcggg ctttggctat gtgctcgaca gtgttagcga gccggacaaa gacgatctgc
    11281 tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca atcgatgccg tttattgatg
    11341 gcacggaaat cgatttcgtt cgtgaaggac ttaatcagat attcaaattt cacaacccta
    11401 aagcccagaa tgaatgtggc tgtggcgaaa gctttagggt ataggcggta ctatgtctcg
    11461 taatactgaa gcaactgacg atgtcaaaac ctggaccggc ggcccgctga attataaaga
    11521 aggattcttc acccagttag ccaccgatga gctggcaaag gggataaacg aagaggtggt
    11581 gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc gtctaaacgc
    11641 ctatcgcgca tggctggaga tggaagaacc gcactggttg aaagcgcact acgacaagct
    11701 gaattatcag gattacagct actactcagc accatcgtgc ggtaattgtg acgacacttg
    11761 cgcgtctgaa cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt
    11821 ggaggcggcg tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg tggcggtgga
    11881 tgccattttc gactctgttt cggttgccac cacttatcgc gaaaaactgg cggagcaggg
    11941 aattattttc tgttcctttg gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata
    12001 tctcggcacc gtggtgccgg ggaatgacaa cttctttgca gcacttaatg cggcggtagc
    12061 ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac
    12121 ctattttcgc attaacgcgg aaaaaaccgg gcagtttgag cgcacaattc tggtggccga
    12181 cgaagacagc tacgtcagct acattgaagg ctgttccgct ccggtgcgtg acagctatca
    12241 gttgcacgcg gcagtggtgg aagtcatcat ccataaaaac gccgaggtga aatattccac
    12301 ggtacaaaac tggttccctg gcgataacaa caccggcggt attctcaact tcgtcaccaa
    12361 gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc
    12421 agcgattacg tggaaatatc ccagctgcat tttgcgcggc gataactcca ttggtgagtt
    12481 ttactcagtg gcgctgacca gcggtcatca gcaagcggat accggcacca agatgatcca
    12541 catcggtaaa aacaccaaat cgaccattat ctcgaaaggg atctctgccg gacatagtca
    12601 gaacagttat cgcggcttag tgaaaatcat gccgacggca accaatgcgc gcaatttcac
    12661 tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga
    12721 atgtcgcaat aacagcgcac aactggagca cgaggcaacg acatcacgta ttggtgaaga
    12781 tcaactgttt tactgcctgc aacgcgggat cagcgaagaa gacgccatct cgatgattgt
    12841 taacggtttc tgcaaagacg tgttctcaga actgccgctg gaatttgccg ttgaagcaca
    12901 aaaactcctc gccatcagtc ttgaacacag cgtcggataa ggaataaaca tgttaagtat
    12961 taaagattta cacgtcagcg tggaagataa agctatcctg cgtggattaa gcctcgacgt
    13021 tcgtcccggc gaagttcacg ccattatggg gccaaacggt tcgggcaaaa gtactttatc
    13081 ggcaacgctt gccgggcgcg aagattatga agtgacgggc ggcacggttg agttcaaagg
    13141 caaagatttg cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt
    13201 ccagtatccg gtggagattc caggcgtcag taaccagttt ttcctgcaaa cggcacttaa
    13261 tgcagtgcgc agctatcgcg gccaggaaac gcacgaccgc tttgattttc aggatttgat
    13321 ggaagagaaa atcgctctcc tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt
    13381 tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg tgctggaacc
    13441 ggagttatgc attcttgatg agtcggactc cgggctggat attgacgcat taaaagtggt
    13501 cgccgatggc gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg ttacgcacta
    13561 ccaacgcatt ctcgactaca tcaagcctga ttacgttcat gtgctatatc agggacgaat
    13621 tgtgaaatcc ggcgatttca cgttggtcaa acaactggag gagcagggtt atggctggct
    13681 taccgaacag cagtaatgcg ctgcaacagt ggcatcactt gtttgaagct gaagggacaa
    13741 aacgctcacc gcaagcacag cagcatttac aacaattgct gcgtaccgga ctgccgacac
    13801 gtaaacatga aaactggaaa tatacgccgc tggaagggct gaccaatagt cagtttgtca
    13861 gcattgcggg agatatatcc ccacagcagc gtgatgcctt agcgttaacg ttagacgccg
    13921 tgcggctggt gtttgtcgat ggacgttacg tgcccgcgct gagcgatgca attgaaggca
    13981 gcggatatga agtgagcatt aacgacgacc gtcaggggtt acccgacgct attcaggcgg
    14041 aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc gccgtgaagc
    14101 gcggtcaacg gccggcaaag ccattgctgt taatgcatat cacccagggc gtggcaggtg
    14161 aagaggtgaa cactgcccat taccggcatc atctggatct ggcggaaggt gccgaagcaa
    14221 cggtgatcga acattttgtc agccttaatg atgctcgtca ttttaccggg gcacggttca
    14281 ctatcaatgt cgcagcgaac gcccacttgc agcatatcaa gctggcgttt gaaaacccgg
    14341 tcagtcacca ctttgcccat aacgatttgt tgctggctga cgatgccacc gcatttagcc
    14401 atagtttcct gctgggtggc gcagtgttac gacacaacac cagtacgcaa ctcaatggcg
    14461 aaaacagcac gctgcggatc aatagcctgg cgatgccggt gaaaaacgag gtgtgtgata
    14521 cccgtacctg gctggaacac aataaaggtt tttgtaacag ccgacagttg cacaaaacta
    14581 tcgtcagcga caaaggccgc gcggtattta acggtttgat caacgtcgcg cagcacgcca
    14641 tcaaaacgga tggtcagatg accaacaata atctgctgat gggcaaactg gcggaagtgg
    14701 atacaaaacc gcagctggaa atctatgcag atgatgtgaa atgcagccac ggcgcgacgg
    14761 tggggcgtat tgatgatgaa cagatgttct atctgcgctc gcgcgggatc aatcagcagg
    14821 atgcccagca gatgatcatt tacgcctttg ctgccgaact gacggaagca ctgcgtgatg
    14881 aggggcttaa acagcaggtg ctggcccgaa tcggtcaacg gctgccagga ggtgcaagat
    14941 gactttttcc gtcgacaaag tgcgggccga ctttccggtg ctttcgcgtg aggtaaacgg
    15001 tttgccgctg gcttatctcg acagcgccgc cagtgcacag aaaccgagcc aggtgattga
    15061 cgccgaggcc gagttttatc gtcatggcta cgcggcggtg catcgcggta ttcatacctt
    15121 aagcgcccag gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc tgtttattaa
    15181 tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg acggaaggga tcaatctggt
    15241 cgccaatagc tggggcaaca gcaatgtgcg ggcgggcgat aacatcatca tcagtcagat
    15301 ggagcaccac gctaacattg tcccctggca gatgctttgc gcacgcgttg gcgcagagct
    15361 gcgtgtgatc cctctcaacc ccgacggtac gctgcaactg gagacgctgc ctacgctgtt
    15421 tgatgagaaa actcgcctgc tggcaattac tcatgtctcc aacgtgcttg gcacagaaaa
    15481 tccactggcg gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc tggtggatgg
    15541 cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg cagggttgcg acttttacgt
    15601 gttctccggg cataaactgt atggccccac cggaattggc attctttatg tgaaagaagc
    15661 cttgttgcag gagatgccgc cgtgggaagg gggcggttct atgatcgcca ccgtcagcct
    15721 gagtgaaggc actacctgga ccaaagcacc atggcggttt gaagccggta cacccaatac
    15781 cgggggcatc attggtcttg gcgcggcgct ggagtatgtt tcggcgctgg ggcttaatag
    15841 catagccgag tatgaacaga atctgatgca ttatgcgcta tcacagctgg aatctgtacc
    15901 ggatctcact ctctatggcc aacaaaacag gcttggcgtt attgctttta atctcggtaa
    15961 acaccacgcc tatgatgttg gcagttttct cgataattac ggcattgctg tgcgtaccgg
    16021 acatcactgc gctatgccat taatggccta ttacaacgtc cctgcgatgt gtcgggcgtc
    16081 gctggccatg tataacaccc atgaagaagt ggatcgtctg gtgaccggcc tgcaacgtat
    16141 tcaccgtctg ctgggataac agggaggcac tatggcttta ttgccggata aagaaaaatt
    16201 gctgcgtaat tttttacgct gcgccaactg ggaagagaaa tatctctaca ttattgagct
    16261 gggccagcgt ctgccagaat tacgcgccga agacggaagc ccacaaaata gcattcaggg
    16321 ctgccagagt caggtgtgga ttgtcatgcg ccagaatgcg cagggaatta ttgaattgca
    16381 gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta ttctctacga
    16441 tcagatgacg ccgcaggata ttgtcaattt cgatgtgcgt ccgtggtttg aaaaaatggc
    16501 gctcacccaa catctcaccc catctcgttc acaaggtctg gaagcgatga ttcgcgcaat
    16561 tcgcgccaaa gccgctgcac ttagctaaac taaagggaca gctttcatcc ggttggctca
    16621 cattgccgga tggtcaatgt ttacactgac ctgcacagga gtgttttggt ttcaggtgaa
    16681 cataagcacc atccgacgtt tcagcgtgag tctccggcaa atacaggagg tttacaatga
    16741 aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt tagcgccatc caggctgcct
    16801 gggcggttga ttatccgcta ccaccaaccg gaagccgact ggttgggcaa aatcaaacgt
    16861 atacggtgca agaaggggat aaaaaccttc aggctattgc ccgacgtttt gatactgcgg
    16921 caatgttgat ccttgaagcc aataacacta tcgccccggt gccaaaacct ggtacgacga
    16981 taactattcc ttcacaactg ttattacctg atgcaccgcg tcaggggatt atcgttaacc
    17041 ttgcagagct gcgcctttat tattatccgc cgggagaaaa tattgtgcag gtttatccaa
    17101 taggtattgg attgcagggg ctggaaacgc cggtgatgga aacgcgtgtt gggcagaaaa
    17161 tccctaaccc aacctggacg cctacggcag gcattcgtca gcgttcgctg gagcgtggca
    17221 ttaaattacc gccagtcgtt cctgccggac caaataaccc gctaggacgt tacgcactgc
    17281 gcctcgcgca tggtaatggc gaatacctca ttcatggcac cagtgcgccg gacagcgttg
    17341 gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc ggatattaaa gccttgttct
    17401 ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga accggtgaaa tattccgtgg
    17461 aacctaacgg gatgcgttat gttgaagtac atcgaccact atcagcagaa gaacagcaaa
    17521 acgttcagac aatgccatac acactgccag caggctttac gcaatttaaa gacaataagg
    17581 ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat ccggtttcgg
    17641 tgagcagtgg agcaactccc gcagccagca atgcgccttc agtagagtca gcgcagaatg
    17701 gtgaaccaga gcaagggaat atgttacgcg cgacgcagta gtggtaaata gcagacaaaa
    17761 aaaatggcgc acaatgtgcg ccatttttca cttcacaggt actattactt gcggtattta
    17821 gtagccatgt tgtccagacg ctggttagca cgagctgcgt catctttagc agcctgaacg
    17881 tcggaacgca ttgcgttcac gtcgttgctc agctggtcaa ctttagcgtt cagagtctga
    17941 acgtcagaag acagctgatc gattttagcg ttgctggagc aacctgccag cagagtagaa
    18001 cccaggatta ccgcgcccag taccagttta gtagctttca ttattaatac cctctagatt
    18061 gagttaatct ccatgtagcg ttacaagtat tacacaaagt tttttatgtt gagaatattt
    18121 ttttgatggg aaggcactta tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt
    18181 ttagataaaa aaattgagag aaaacaggat gcttccatcg gattcatctt aaataaagtg
    18241 agattatata gtgaaatccg atttg
//
LOCUS       DADLTI010000076        18074 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLTI010000076 DADLTI010000000
VERSION     DADLTI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.076"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..556
                     /locus_tag="JN755_002346"
     CDS             <1..556
                     /locus_tag="JN755_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7381115.1"
                     /translation="RFNRPSSLFARCWLLVEGETETWVINELARQCGHHFDAEGIKVI
                     EFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATVRSLLNNDREAEREHLTALPA
                     LDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRG
                     VDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(553..1509)
                     /locus_tag="JN755_002347"
     CDS             complement(553..1509)
                     /locus_tag="JN755_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7381116.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            1660..2775
                     /gene="macA"
                     /locus_tag="JN755_002348"
     CDS             1660..2775
                     /gene="macA"
                     /locus_tag="JN755_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7381117.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            2772..4718
                     /gene="macB"
                     /locus_tag="JN755_002349"
     CDS             2772..4718
                     /gene="macB"
                     /locus_tag="JN755_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY7381118.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(4791..5015)
                     /gene="cspD"
                     /locus_tag="JN755_002350"
     CDS             complement(4791..5015)
                     /gene="cspD"
                     /locus_tag="JN755_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7381119.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            5345..5665
                     /gene="clpS"
                     /locus_tag="JN755_002351"
     CDS             5345..5665
                     /gene="clpS"
                     /locus_tag="JN755_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7381120.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5696..7972
                     /gene="clpA"
                     /locus_tag="JN755_002352"
     CDS             5696..7972
                     /gene="clpA"
                     /locus_tag="JN755_002352"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7381121.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(8375..8462)
                     /locus_tag="JN755_002353"
     tRNA            complement(8375..8462)
                     /locus_tag="JN755_002353"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8426..8428),aa:Ser,seq:gga)
     gene            complement(8716..8934)
                     /gene="infA"
                     /locus_tag="JN755_002354"
     CDS             complement(8716..8934)
                     /gene="infA"
                     /locus_tag="JN755_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7381122.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(9219..9923)
                     /gene="aat"
                     /locus_tag="JN755_002355"
     CDS             complement(9219..9923)
                     /gene="aat"
                     /locus_tag="JN755_002355"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7381123.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(9965..11686)
                     /gene="cydC"
                     /locus_tag="JN755_002356"
     CDS             complement(9965..11686)
                     /gene="cydC"
                     /locus_tag="JN755_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7381124.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQQVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11687..13453)
                     /gene="cydD"
                     /locus_tag="JN755_002357"
     CDS             complement(11687..13453)
                     /gene="cydD"
                     /locus_tag="JN755_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7381125.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13576..14541)
                     /gene="trxB"
                     /locus_tag="JN755_002358"
     CDS             complement(13576..14541)
                     /gene="trxB"
                     /locus_tag="JN755_002358"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7381126.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            15086..15580
                     /gene="lrp"
                     /locus_tag="JN755_002359"
     CDS             15086..15580
                     /gene="lrp"
                     /locus_tag="JN755_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7381127.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15715..>18074
                     /locus_tag="JN755_002360"
     CDS             15715..>18074
                     /locus_tag="JN755_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="DNA-binding membrane protein required for
                     chromosome resolution and partitioning; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7381128.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAP"
BASE COUNT         4564 a         4480 c         4852 g         4178 t
ORIGIN      
        1 tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg ttgctggtgg aaggggaaac
       61 ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg
      121 gatcaaggtc attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccgccg
      181 aatggggatt gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc
      241 tacggtacgc agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt
      301 accggcgctg gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg
      361 cgtggcgcaa atcccggaaa atgtaccgat gaatctacgc aaaattatct cgaaagcgat
      421 ccatcgctct tccaaacccg atcttgccat tgaagtggca atggaggcag gacgtcgtgg
      481 tgtggactcc gtaccaacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg
      541 cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat
      601 ctcatagcgc cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg
      661 tgctatctgt gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgactc
      721 ccagaacgcg ttgtaatcag catggatctt gccttcttta tcgcgataac gcaggctgcg
      781 gtaaatatgc gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa
      841 cagacaggcc gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc
      901 attctggatt tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat
      961 cgttcttttc ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc
     1021 gctgttgcgg aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag
     1081 ctcaagcgtg aactgctcac cgtttttgcc ttccagttta gccagttgca gcccgggggt
     1141 attcaaatat aagctgaatt cttccgccga catacacccg cggagtaacg cataatggta
     1201 acgtaacgcc tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgcag
     1261 acgcacaggc agtcgcggct ggcgcgttaa caacacattg agattaggcc agtgggaaag
     1321 ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg
     1381 gcgaaaacta cagcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag
     1441 aaaaagtgac aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga
     1501 catattcatg aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca
     1561 aacatcaata gtgtgattac taacgtaaat tttagggttt tgttgatatt tcgttgaagt
     1621 taatgacccg gattggcatg tggagtattc agaatattta tgaaaaagcg gaaaaccgtg
     1681 aagaagcgtt acgttattgc gctggtgata gtcatcgccg gactgattac gttatggaga
     1741 attcttaacg cacccgtgcc gacttatcag acactgattg tgcgccccgg tgatttacag
     1801 caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta aggttgacgt aggcgcgcag
     1861 gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag
     1921 cttttagggg tcattgatcc tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg
     1981 ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg
     2041 acgtattccc gtcagcaacg tctggcacaa acgcaagcgg tttcactgca ggatctcgat
     2101 accgccgcga cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc
     2161 aagcgcaatc aggcctctct cgatacggct aaaaccaatc tcgattacac ccgtatcgtt
     2221 gccccgatgg gcggggaagt gacgcaaatc accactctgc aaggccagac ggtgattgcc
     2281 gcacagcaag caccgaacat tctgacgctg gcagatatga gcactatgct ggtaaaagcg
     2341 caggtttctg aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggtg
     2401 cttggcgatc cactgacgcg ctacgagggg caaatcaagg atgtgctacc gacgccggaa
     2461 aaggtaaacg acgctatttt ctattacgcc cgttttgaag tacccaaccc caatggtttg
     2521 ctgcggctgg atatgaccgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgttg
     2581 acgatccctc tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg
     2641 ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat
     2701 gttgagattg tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca
     2761 ggagctgcac aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg
     2821 gtgatgagca ggttgaggtg ctgaagggca tcagcctcaa tatttatgcg ggtgagatgg
     2881 tcgcgattgt tggcgcttcg ggttccggta aatcgaccct gatgaatatt ctcggctgcc
     2941 tggataaggc caccagcggc acctatcgcg tagccggtca ggatattgcc acgctggacg
     3001 ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt
     3061 tgctttcgca tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg
     3121 agcggaaaca gcgactgctt cgtgcccagg agttgctgca acggctgggg ctggaagacc
     3181 gtacagagta ttatccggca cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc
     3241 gggcattgat gaacggcggt caggtaattc ttgccgatga accgaccggc gcactggaca
     3301 gccattctgg cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg
     3361 tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc
     3421 gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt gctggcggga
     3481 cggagcctgt tgtcaataca gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg
     3541 cgctgacgat ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc
     3601 tggggattat tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca
     3661 aacaaatggt gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg
     3721 ggaaagattt tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa
     3781 tcgccatcca aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc
     3841 gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt
     3901 ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt taatcaggag cagctgaacg
     3961 gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag
     4021 cagatgtggt tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg
     4081 cggaagaaaa acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca
     4141 gcacgatgtc cgggcgagtt atgggccagt cgtggcttaa ctccattact gtcagggtga
     4201 aagaaggatt tgacagcgcc gaggcggaac agcaactcac gcgtttactt tcactgcgcc
     4261 acggtaagaa ggatttcttt acctggaaca tggacggcgt cttgaaaact gttgaaaaga
     4321 ccacacgtac tttacaactg tttctgacgc tggtggcggt gatttcgctg gtggtgggcg
     4381 gtattggtgt aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca
     4441 ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca acagttcctg atcgaagccg
     4501 tactggtttg cctggtcggt ggcgcgttgg gaataacact gtcactgtta attgctttca
     4561 ccttgcagct tttcttaccc ggctgggaga ttggtttttc accgttggcg ctgctgctgg
     4621 cgtttctctg ctcgacggcc accgggattt tatttggctg gttacccgca cgaaatgcgg
     4681 cacgactgga tccagtagat gctctggcac gggagtaatt tttgagataa aaatgccagc
     4741 cgatcgggct ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact
     4801 gccgcttcta cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg
     4861 acatcaaact gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggtg
     4921 gaataatgag cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaacctttg
     4981 gcattgttga accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta
     5041 tacaagtaag atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc
     5101 tagaaataca atttagagaa ttagggcgag ccgtcaagca tttgacaggg gccaggggcc
     5161 agggggcagg tatgaatcaa aaatttgaag cagttaacgc tattgacagg aatgtgacag
     5221 atgtcgctga tgccaacgat agatgatagt tatctatcat gtggagtaga ttggtcaggc
     5281 aaataagctc ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa gatgataact
     5341 gacaatgggt aaaacgaacg actggctgga ctttgatcaa ctggcggaag aaaaagttcg
     5401 cgacgcgcta aaaccgccat ctatgtataa agtgatatta gtcaatgatg attacactcc
     5461 gatggagttt gttattgacg tgttacaaaa attcttttct tatgatgtag aacgtgcaac
     5521 gcaattgatg ctcgctgttc actaccaggg gaaggccatt tgcggagtct ttaccgccga
     5581 ggttgcagaa accaaagtgg cgatggtgaa caagtacgcg agggagaatg agcatccatt
     5641 gctgtgtacg ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg tgcctatgct
     5701 caatcaagaa ctggaactca gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca
     5761 tgagtttatg accgtcgagc acttgttact ggcgctgctc agtaacccat ctgcccggga
     5821 ggcgctggaa gcgtgttctg tggatttggt tgcgctccgt caggaactgg aagcctttat
     5881 tgaacaaacc acacccgttc tgcctgccag tgaagaggag cgcgacacac agccgacgct
     5941 gagttttcag cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga
     6001 ggttaccggt gcaaacgttc tggtcgctat cttcagcgaa caggagtcgc aggcggcata
     6061 tctgttgcgt aaacatgaag tcagccgtct cgatgtggtg aactttatct ctcatggcac
     6121 gcgtaaagac gagccgacac agtcttctga tcctggcagc cagccaaaca gcgaagaaca
     6181 agctggtggg gaggaacgta tggagaattt cacgacgaac ctgaaccagc ttgcgcgtgt
     6241 gggcggaatc gacccactaa ttggtcgtga gaaagagctg gagcgtgcta ttcaggttct
     6301 ctgccgtcgc cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc
     6361 gattgcagaa ggtcttgcct ggcgaattgt tcagggtgat gtgccggaag tgatggctga
     6421 ctgtacgatt tactctctcg atatcggttc tctgttagcg ggcacaaaat atcgcggcga
     6481 ctttgaaaaa cgttttaaag cgttgctcaa gcagctggag caggacacta acagcatcct
     6541 gtttattgat gagatccaca ccattatcgg tgcgggtgca gcgtctggtg gtcaggtcga
     6601 tgcggctaac ctaatcaaac cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac
     6661 aacctatcag gagttcagca acattttcga gaaagaccgt gctctggcgc gtcgcttcca
     6721 gaaaattgat attactgaac cgtcgatcga agaaactgtt caaatcatca atggcctgaa
     6781 accgaagtat gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc gtgcggcggt
     6841 agagctggcg gtgaaataca ttaacgatcg tcatctgccg gataaagcca ttgatgttat
     6901 cgacgaagcg ggcgctcgcg cacgtctgat gccggtaagc aaacgcaaga aaaccgttaa
     6961 tgtggcggat attgagtccg tggtggcccg tattgcacgc attccagaga agagtgtttc
     7021 tcagagtgac cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg
     7081 ccaggataaa gccattgagg cgctgactga agccattaag atggcgcgtg caggtttagg
     7141 tcacgaacat aaaccggtcg gttcgttcct gtttgccggt cctaccgggg tcgggaaaac
     7201 agaggtgacg gtacagcttt cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc
     7261 cgagtatatg gaacgccata ccgtcagccg tctgattggt gcgcctccgg gatacgttgg
     7321 ttttgatcag ggcggtttgc tgaccgatgc ggtaatcaag catccacatg cggtgctgct
     7381 gctggacgaa atcgagaaag cgcatccgga cgtgttcaat attctgttgc aggtgatgga
     7441 caacggtacg ctgaccgata acaacggacg caaagcggac ttccgtaacg tggtgctggt
     7501 gatgaccact aacgccgggg tacgggaaac tgagcgcaaa tccattggtc ttatccacca
     7561 ggataacagc accgatgcga tggaagagat caagaagatc tttacaccgg aattccgtaa
     7621 ccgtctcgac aacattatct ggttcgatca tctgtcaact gacgtgatcc atcaggtggt
     7681 ggataaattc atcgtcgagt tgcaggttca gctggatcag aaaggtgttt ctctggaagt
     7741 gagccaggaa gcgcgtaact ggctggccga gaaaggttac gaccgggcaa tgggcgcacg
     7801 tccgatggcg cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt
     7861 tggttcgctg gtggacggcg gtcaggtgac ggttgcgctg gataaagaga aaaatgagct
     7921 gacttacgga ttccagagtg cacaaaagca caaggcggaa gcagcgcatt aatctgattg
     7981 tcaggtaggt tggtgaagtc cgtaatctcg tcaggggtta cggacttttt atttatgggg
     8041 tggaacggtg aaaaccctat ttttggaggt gaaggtaagt tgttgataat tagtgctgct
     8101 ggaaggtaag gataaaaaag ggtgctgcag gagaatggga tggttttgct ttattaacaa
     8161 cgggctaaac gtgtagtatt tgagttcact gccgtacagg cagcttagaa atcgacgggg
     8221 tgcggtaaaa cctttgcgaa cgcgttcact gccgtacagg cagcttagaa attcacaggt
     8281 aacatactcc acccgcccac catgttcact gccgtacaga cagataaaat gcgaaaaaaa
     8341 agctcgcact ttcgtacgag ctcttcttta aatatggcgg tgaggggggg attcgaaccc
     8401 ccgatacgtt gccgtataca cactttccag gcgtgctcct tcagccactc ggacacctca
     8461 ccaaattgtt ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg
     8521 agtaaaacgg tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact
     8581 tgtgcagtat atctacatcg agacaagtta tggacttata cttccaaagt acttcataca
     8641 tatcacaaaa taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat
     8701 caggcggtaa aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg
     8761 ggtcagttca acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt
     8821 accggagatg tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt
     8881 aggcaacgtt tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc
     8941 ctctggggta tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa
     9001 gtaaagattt gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac
     9061 ggctaagccg caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta
     9121 cctgacgcga aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg
     9181 caattataac actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag
     9241 caccgtggta cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta
     9301 aggtaatccc ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc
     9361 tggcagtcga taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc
     9421 gttttagacg cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg
     9481 gccacgccgt acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca
     9541 tgaccgagtt cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct
     9601 tcttcgcgat cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca
     9661 cgatagggcg agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc
     9721 catagcaccg cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac
     9781 ggaaaaatac cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt
     9841 gccagcaggc cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg
     9901 cgagaaagct gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa
     9961 tagcttacaa accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt
    10021 gagtaccttg ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga
    10081 gtccgcgaag tcgatgggtg accattaaca ccgttttctc acgcatcatt tttgcaagca
    10141 attcaaggat ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca
    10201 ccagtggcgc atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg
    10261 agagctggcg tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct
    10321 ccagaccaac gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga
    10381 gtaacagatt atcacgcagc gtggcgctaa acagatgcac ttgctgagga acaacgctga
    10441 tggtctgtcg tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct
    10501 cgccctgttg cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc
    10561 cggttcgccc gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa
    10621 gtgcctgttg ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc
    10681 gatcggcaac acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt
    10741 cagagatacg tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg
    10801 gtgccagtgc ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg
    10861 gttgagcatt gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg
    10921 cgccaatgag cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg
    10981 cttccagcca ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa
    11041 aaatggtcag ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc
    11101 gaagatgagt cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca
    11161 ggaaaagcgt cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac
    11221 ttaacccgat tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc
    11281 gcaggtaaag atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct
    11341 gacgatagcg cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc
    11401 gcagatgctg caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc
    11461 cggcagtacg ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc
    11521 tgtacagtcc ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca
    11581 acagaccgat actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc
    11641 atttatgacg tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct
    11701 gacgatgggc cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc
    11761 cttgctcaat aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt
    11821 ctaactggtg ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca
    11881 ttacgcgctg ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg
    11941 aacagggatt tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc
    12001 gggcagcctg gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc
    12061 tgacccaggc gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca
    12121 gtagtacgtt atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga
    12181 gatgtttacg ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg
    12241 atccctgata tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc
    12301 tgcggccaac caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca
    12361 gcgttttacc ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg
    12421 tcaatgctaa ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt
    12481 tcagactgtc agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc
    12541 gtaatggctg gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca
    12601 ccccagtatc gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg
    12661 ccaccagagc aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca
    12721 gtcgtagcac ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa
    12781 tttcagcttc accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga
    12841 aatgcccact taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca
    12901 ttccaaccag cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg
    12961 cagcccagtt agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg
    13021 ccatttgcgg cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca
    13081 gcgtcgccca gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac
    13141 ggtcgagaac ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc
    13201 gttcgcgtaa ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg
    13261 gaagcagcag ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca
    13321 tgaaccaggc ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat
    13381 tcagccaacg ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac
    13441 gagatttatt cattgcacgc ttaacaggtg agttatcaga attatttgca gagcaatgtt
    13501 acaacgggga aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt
    13561 tactgatttg taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag
    13621 tgctgccatg cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac
    13681 gtcgcctgcg gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga
    13741 ctgtactttg atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg
    13801 gctgtgaccg atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt
    13861 ttgcgtatcg cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag
    13921 cgtacggttg gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat
    13981 aaggattttt tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat
    14041 gttagacaga tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt
    14101 ctggttgcga tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa
    14161 ggcttcttca gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc
    14221 gtcgcaagtg tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac
    14281 cttgttgata tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg
    14341 cgccattaat aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt
    14401 ggtggtggtc agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc
    14461 gcgcgccgcg tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt
    14521 actgtgtttg gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg
    14581 tagggaattt acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg
    14641 gcagcatcga taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc
    14701 cgctttataa gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt
    14761 gtactaaaaa ttgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg
    14821 aagaaaaaaa acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg
    14881 tgctagtgaa atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac
    14941 aaggcaacgg tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc
    15001 ggtctatcgt gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg
    15061 gaaggaatac agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct
    15121 cgaccgtatc gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt
    15181 cgagctttct aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct
    15241 ggaaagacaa gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc
    15301 atcacttctg gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca
    15361 attcaatacc gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga
    15421 tttcgactac ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg
    15481 ggaaaccctg ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga
    15541 agtcaagcag agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc
    15601 ggcgtatttt gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta
    15661 ctgttgtccg ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc
    15721 caggaataca ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt
    15781 ctggaggcgt tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta
    15841 agctttaacc cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat
    15901 ttaggtggga tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg
    15961 gcttacacca ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc
    16021 agcgacgaat acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc
    16081 atccttacct cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc
    16141 ggtggcgtca ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg
    16201 ggaactattg cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca
    16261 tgggtgacca ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt
    16321 aatcgtaccc gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag
    16381 tatgaagatg aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc
    16441 gcgctagcgc gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca
    16501 gacgctgcgt tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca
    16561 cgcggtgtgg ctgccgaccc ggacgacgtc ctatttttgg gcaatcgtgc aacgcagcca
    16621 gaatatgacg aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta
    16681 gcagctgctg ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag
    16741 acgccgcctg ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct
    16801 gtaccgggtc cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag
    16861 cagtcacaat atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag
    16921 ccgcagcagc cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct
    16981 cctgcggcgg aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg
    17041 caacagccgt attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc
    17101 gaagagcagc aatccatttt tgctccacag tctacatacc agactgagca aacttatcag
    17161 cagccagccg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt
    17221 gtggagcctg aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt
    17281 gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg
    17341 attccagaac cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt
    17401 gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa
    17461 aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca
    17521 aatagcggtg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg
    17581 aaacgtatcc gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca
    17641 cagcgtgcgg cggaagaaaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat
    17701 cagtataacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc
    17761 cagacacagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa
    17821 gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt ttacgcaaac tcaacaacaa
    17881 cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt
    17941 tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt
    18001 gaacctgtac agcagccgca acaaccggtt gcaccacagc agcagtatca gcagccacaa
    18061 cagccggttg cgcc
//
LOCUS       DADLTI010000077        17891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLTI010000077 DADLTI010000000
VERSION     DADLTI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.077"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..720
                     /gene="nikC"
                     /locus_tag="JN755_002361"
     CDS             <1..720
                     /gene="nikC"
                     /locus_tag="JN755_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7381129.1"
                     /translation="IDLPSCLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACL
                     LLVLILGLVIGGSAGLIGGRVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIA
                     IALSHWAWYARMVRSLVISLRQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATL
                     DIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAF
                     NLVGDALRDHLDPHLVTEHAH"
     gene            720..1484
                     /gene="nikD"
                     /locus_tag="JN755_002362"
     CDS             720..1484
                     /gene="nikD"
                     /locus_tag="JN755_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7381130.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            1481..2287
                     /gene="nikE"
                     /locus_tag="JN755_002363"
     CDS             1481..2287
                     /gene="nikE"
                     /locus_tag="JN755_002363"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7381131.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            2293..2694
                     /gene="nikR"
                     /locus_tag="JN755_002364"
     CDS             2293..2694
                     /gene="nikR"
                     /locus_tag="JN755_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7381132.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(2814..3173)
                     /locus_tag="JN755_002365"
     CDS             complement(2814..3173)
                     /locus_tag="JN755_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7381133.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(3170..3445)
                     /locus_tag="JN755_002366"
     CDS             complement(3170..3445)
                     /locus_tag="JN755_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7381134.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(3504..4628)
                     /locus_tag="JN755_002367"
     CDS             complement(3504..4628)
                     /locus_tag="JN755_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7381135.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(4628..7363)
                     /gene="rbbA"
                     /locus_tag="JN755_002368"
     CDS             complement(4628..7363)
                     /gene="rbbA"
                     /locus_tag="JN755_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7381136.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(7360..8427)
                     /locus_tag="JN755_002369"
     CDS             complement(7360..8427)
                     /locus_tag="JN755_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7381137.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(8793..10415)
                     /locus_tag="JN755_002370"
     CDS             complement(8793..10415)
                     /locus_tag="JN755_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7381138.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(10677..12266)
                     /locus_tag="JN755_002371"
     CDS             complement(10677..12266)
                     /locus_tag="JN755_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7381139.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            12762..13681
                     /locus_tag="JN755_002372"
                     /pseudo
     CDS             12762..13681
                     /locus_tag="JN755_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(13768..14261)
                     /locus_tag="JN755_002373"
                     /pseudo
     CDS             complement(13768..14261)
                     /locus_tag="JN755_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14215..14979)
                     /locus_tag="JN755_002374"
     CDS             complement(14215..14979)
                     /locus_tag="JN755_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7381140.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            15226..15987
                     /locus_tag="JN755_002375"
     CDS             15226..15987
                     /locus_tag="JN755_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7381141.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(16094..16606)
                     /locus_tag="JN755_002376"
     CDS             complement(16094..16606)
                     /locus_tag="JN755_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381142.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(16745..>17891)
                     /locus_tag="JN755_002377"
     CDS             complement(16745..>17891)
                     /locus_tag="JN755_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7381143.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         4685 a         4383 c         3940 g         4883 t
ORIGIN      
        1 attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg caccgatcac
       61 ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct cggttcggta
      121 atggcctgcc tgctgctggt gctgatatta gggctggtta ttggcggcag cgccgggttg
      181 attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat gaccttcccg
      241 acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac caacgtaatt
      301 atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag cttggtgatt
      361 tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg ccatgtgcgg
      421 gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct ggcaacgctg
      481 gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct cggtgtgacc
      541 gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat ctggacccag
      601 ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc ctttaacctg
      661 gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca cgcacactaa
      721 tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg ctggtacacg
      781 gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt agcggcagcg
      841 ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt cgccagacgg
      901 cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc ggcatcaaaa
      961 ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc atgcacaccc
     1021 acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg cttaccgctg
     1081 ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac ccgttcgaga
     1141 tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt gaatcaccgt
     1201 ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg cgcatcctcg
     1261 atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc acccatgata
     1321 tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt aaaattgtcg
     1381 aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg cgcagcctgg
     1441 tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac ttaacgtctg
     1501 cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac atcaggcggt
     1561 gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc tgggacgcag
     1621 cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc cctcacaagg
     1681 gaatattagc tggcgcggcg aatcgctggc gaaacttaat cgcgcccaac gtaaagcctt
     1741 ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc cgcgcaaaac
     1801 cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa aatccgaaca
     1861 actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg ttctcgacaa
     1921 acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac gcgcgctggc
     1981 ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc tcgtgttaca
     2041 ggcgggcgtc attcgcctgc tgaaaaatct acaacaacag tttggcactg cctgcctgtt
     2101 catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg tgatggacaa
     2161 cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct ctgacgccgg
     2221 acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca cctcagaaaa
     2281 ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact ggagacgctg
     2341 gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg cgatattctg
     2401 cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt cgcggtgctg
     2461 tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc cacccagcat
     2521 catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga cgactgtctg
     2581 gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga tgacgttatc
     2641 gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga ttgagttatt
     2701 tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta agttcagttg
     2761 catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa aatttataaa
     2821 tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac ttgttgctgc
     2881 gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt aaaggtctta
     2941 atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc aagatactct
     3001 cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac aaaatcacaa
     3061 taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac gtaggtaata
     3121 acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat catggtttta
     3181 cccctatact tagtaaccag tcacgcacgc tttctacagc gcctttatcg gtatctggcg
     3241 acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg caacgcgaac
     3301 ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata tcagaccact
     3361 tgatcccttg aggaacaggt gttttaaata tttgatccaa tgtatttttt tgtttcttcc
     3421 gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta tgacatcaca
     3481 gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct gaatcgcagc
     3541 agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg cggccagacg
     3601 atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa gtgtgtcgtc
     3661 ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg cgtggatcca
     3721 ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag ttgcggcatt
     3781 gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag actgagcgcc
     3841 acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac acctttcacc
     3901 atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga ccagatcttc
     3961 gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac cgtgccgtgt
     4021 tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt aatgttgttg
     4081 atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa gcgcatccgg
     4141 gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa gctgttcact
     4201 tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct catgcgcgtg
     4261 gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg ctgaaaatta
     4321 ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc catctcatcg
     4381 gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac gatccggttc
     4441 gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa cgatcctggc
     4501 gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag cgtcagcatc
     4561 gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat attgcgtaac
     4621 tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc accagcggga
     4681 tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc gctttcgaga
     4741 acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg atccaacgtc
     4801 caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc gccgggatca
     4861 atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag gtggagatca
     4921 gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg agcgtcagga
     4981 aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc aggaaaaagt
     5041 tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg ctacgggtgg
     5101 tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc acgctaaggg
     5161 cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga acaatcgctg
     5221 gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc agcccgcttt
     5281 ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg tgcattgcct
     5341 gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc cagacgccga
     5401 gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc tcaatggcca
     5461 ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg agcggcggct
     5521 gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta ctgacggtct
     5581 ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg ccgtaaccca
     5641 ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact ggatcgcgtc
     5701 gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta aatccctgac
     5761 gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg ctctgccctg
     5821 ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg cgtttctcaa
     5881 ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt gagatgcggt
     5941 cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact ttgtcctggc
     6001 gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg acaccagaag
     6061 taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc agcgaaagcc
     6121 gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg ttgagcttaa
     6181 aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg tgaaacaaac
     6241 gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa aacgcctgcg
     6301 acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt tgcccaaata
     6361 gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg gtggtggatt
     6421 taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg cggaaattga
     6481 cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct tcgatggcaa
     6541 tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc tggcgttgcg
     6601 cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt tgctgccgca
     6661 gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc agccagtcga
     6721 agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg ctctgccgct
     6781 ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca acccccgttg
     6841 ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac agcccaagtt
     6901 tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt gctaacccgg
     6961 tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga ccgaacaggc
     7021 gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt ttgcccagcc
     7081 cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg tcgtgcatat
     7141 cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa atcagtgaca
     7201 ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa cgggccggaa
     7261 tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc acgcccgcca
     7321 gttgcgcgac aggcgggaca ggaaccagtt ccagatgcgt cattgcggca acctcaccac
     7381 gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc ccggtaaacc
     7441 ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg ctttgacgcg
     7501 gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga actgggcgac
     7561 actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat cgaggatcag
     7621 ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga agaaggtcat
     7681 atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca gcacttcccc
     7741 tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt cactgtcatc
     7801 gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag tttgcgcctg
     7861 aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct gggctttcgc
     7921 tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca gctgttgcgc
     7981 agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg caacagagtc
     8041 cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac tttgtcgttg
     8101 ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt gcgcgatggc
     8161 ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt cgccttcgcg
     8221 aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcagttt tgctggcgat
     8281 atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg gcacacccgc
     8341 cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga caacccacca
     8401 cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt aagcaaacga
     8461 cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg gataagaagg
     8521 aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg cttagcgaaa
     8581 acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac gcatactcat
     8641 aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat cgcgtttcac
     8701 gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag tctcaaccag
     8761 atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt gcgccttcta
     8821 atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca gggagtggtt
     8881 atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat tcttccagca
     8941 tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat gcgttctctc
     9001 ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga acatgctaat
     9061 agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt aaaggatcga
     9121 caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg atgcttttta
     9181 ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc aatagagaca
     9241 gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc atgatgattt
     9301 gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat cccgtcgtag
     9361 gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc attgcaattc
     9421 gtttcatgat ttccagctaa tacatgaaca tttttattga tactcattat gcgttttaac
     9481 aaatcgatga taaattgaac gccacagata ttactaaatc gatcgccggt ttggtcgcca
     9541 agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg aagactttct
     9601 aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt gtcaagttgc
     9661 tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt aagcaactgg
     9721 ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa aagcgcgtac
     9781 aaaactgcac cgactgagct atcggtatcg ccaaaatagg tcggcctggt acttgtggga
     9841 tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt tataattgtt
     9901 tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat gggtttgtac
     9961 gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt aagtaaataa
    10021 acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtgatt tttactatta
    10081 attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg catgcccgat
    10141 agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt gtcatcttga
    10201 atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac ttcaccgagc
    10261 attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata cccaaacagt
    10321 tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat tgtcgttgta
    10381 ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt attcaccgcg
    10441 aggaatacat agttaacatt ttatcttatg acatacacct catattcgat gggaaaataa
    10501 ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt atctcatagc
    10561 agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca atcgattaag
    10621 aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat gaagttttat
    10681 tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc gttaggtgct
    10741 atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg gtctacccct
    10801 aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca tttgaagata
    10861 tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata tacaggtgtg
    10921 ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg ttgtttatgt
    10981 tttttattaa atatatttgc taattcctca ggatcaattg ggtctttcat ctgattaaat
    11041 ggaacctgca aagcaccgag acaataacat tgactttctt catttcttat tattccgtga
    11101 tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt tttaattaag
    11161 ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata gttaccatta
    11221 aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg gctatcttta
    11281 ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc accaaaaata
    11341 gtactaaatc tatcgcctgt atgatcccca agggggatac aaggggttat cgcatcgtcc
    11401 tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt taataaattg
    11461 tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc gtgttttaat
    11521 aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga aacgagcaat
    11581 gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt gttggcattt
    11641 tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac agatttaaca
    11701 tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg ccagtcagct
    11761 ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag cccattaact
    11821 ttataaatta tgtctgattt atctccggag aaattaataa aataataacc atccttacac
    11881 tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc tggtataatt
    11941 gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg aatatctata
    12001 gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc atttaccctc
    12061 cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc tatttgcgaa
    12121 tcattatcca gtttatcaat ttcaactatc tttgaacgat gtgttttatc aaccttgcta
    12181 tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga agggatgtac
    12241 ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc tgttagtaaa
    12301 aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat aatacttata
    12361 tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg gaaaacaatc
    12421 catccctgga agacaattgt aaaaaaaaca acagaataag aattaatctt aggataaata
    12481 tttatttatc atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta
    12541 tagttatgct atacatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt
    12601 atcattaatg cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata
    12661 tatcgggtag cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg
    12721 tggctttgtg ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt tctctggcgg
    12781 ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcagctc acgcgcggcg
    12841 ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca
    12901 tgatatgggg ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg
    12961 gccatgttat ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt
    13021 ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg
    13081 tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc
    13141 tgctcggttt catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg
    13201 tcgccttggg ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca caatcgtcca
    13261 tcaaacacgt aatacgttct catccaaaga gcactggctg tggtgttatt gggttatttt
    13321 tctcggctca gtcacggtgc tgcaggggat atacgtctta gtcagttccg atgcaagcgc
    13381 ccgactggct cccggcatta ttctcatttg cctcggaatg atctgttaca gcatattctc
    13441 aaaagtctgg ctactggcac tggtctggag acgtacctgt tcgttagcca acagaatacc
    13501 gatgataccg gtatttacct gtctgttttg cttgtttttg tcatcattcc tcgcggaaat
    13561 cgcacaaact gagctggact actttattcc atctcgggtt ctggttggat taagcgcagt
    13621 atgttttaca ttgttctcta ttgtttcaat attagaggcc ggttctgcga aaaaaaatta
    13681 aaatttacat aatcccagcg ttattcatat cttaacgctg gttttttacg aacttagtga
    13741 aataatgtag aatataaaat tataatttta tattggagca catgtgttaa tgatcaaccc
    13801 cgggatacta aaaatataga cattattaaa cttgcttagc ctcgcattgc ctttacctgt
    13861 taaagatgca tcgagaaaat tgagtctgaa gttctcatcc tttatctgat tttccagtcc
    13921 cgaaactttg gccttagcat tacctgagat ccgtccgtta ctatctattt tgtaggtaaa
    13981 aaaatacgaa tgggaaaatt tcctcggtgt atcttcatca gttaagccaa caatcgtaaa
    14041 actaccgcta ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt ataaagaatg
    14101 atctgtttta gcatttcttg ttaacgtcga cgtacattca agagacgttg tgctaccatc
    14161 acgagccttc cagagagcaa aaaaacacac ctgtagcaaa aatacatttt cttattagtc
    14221 atataatagc acctctgaac aaattgcata agtttcgcca ttagaggatt ttatacatct
    14281 tgataaatac cgctgccctt ccccaagcaa ataatgcata ctatttacat catatataaa
    14341 atttgcacct ggcacacagg gtaaatgctt tttcgaaatt tcagcggcct ctatttgaga
    14401 tttagcgagg ccaatctcgt taagattcgc tgaaagatat aaatcacagg tgtcaatctt
    14461 acctgattta accaatggat aatccgttat actgtaggta tctttaaaag ccacggcaaa
    14521 catttcaata ataacaatta ctaccacaag cgaaatcaaa atgcgaaaaa gaggaatact
    14581 ccttttgcgc tctacttttt caacaacatc ttcttgtaaa tgagaatcat cataagattg
    14641 tttacttatt gttatgtttt cactgatgta aaagcctact ttgggtatcg taagaatcac
    14701 attttcttca acaccaatat ttttaaaact tttgcgtaac agggatatat attggttcaa
    14761 tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg ctatcaatga ttgagttcct
    14821 gtcaaccgct ccaccattac tttccagtaa cactgccaaa atacaccccg ccgtagctgg
    14881 caaatttatc tcatcctggg tatttacatt cgagagcgag catttgttcg gatcaaaaat
    14941 aatcgcgtca ttgataatga aaaaacatct ccgctccatg caggcaacaa cacaaaataa
    15001 gattttgtca ttttagccta ttcctggtgg aactgacaca agaaactaaa atacaaatat
    15061 aaccctgaaa aagtcatata aaacttatat ccacagaata aaaaaacaaa acatgtcagt
    15121 tttatcaata taaagtatcg caaggcgaaa agtgatgcaa gaatactcct aagtaaatga
    15181 aagcgcacca catgaaatta tcaacacatg taatttcatc attaggtgct atcgaaaata
    15241 actcaggagc tacttaagat gaataatgtc ttactcatga gacccgatga ctcagaattt
    15301 atgatacctg atgactggtc agggctaaac gcaaaaggca ttacatacga acaagataat
    15361 agagtgacag agtcttttct gccttttaaa tctttttatt gttcaccggt gtgcacgaca
    15421 gttgaactca tctcacaaaa taattgtttt gctatgggta tccgctacct tctggaaaag
    15481 aaaagagact taaggctaaa tcactttaaa gccttctctc ataaatgcga gacaaaacct
    15541 gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt atagtatatt ctcacgcatt
    15601 gttgaagaaa atataatgaa aacgatgaca cctatcgtca tcattactgg gcatcactca
    15661 gaagatcttt gttctctggc atcgaaactg ggaggagtta aaataatatc accgacagaa
    15721 aagattgcaa aaatagaaaa cgctatcaat gatgctttta acagcaagcc taaaaaaata
    15781 agatttttaa cagatatact ctctcttcaa caaattgaga ccatgaaaat ggttgcgcag
    15841 ggtagatccg ttaatgagat ttcagccgca atgaatatta gcatcaagac cgtgtatgca
    15901 cataaagttc gtgctatcaa taagctggga attaagaaaa aagtttatga agcgcttttt
    15961 tataaagcgc taattcagtt caactgatgt aaaaatcacc ctcgcttaat aaataatata
    16021 ttctatgtct ttttttataa gttaaaaaag gcgggatatc aaatcccgcc atgaaattaa
    16081 ctaatcatct gcattattct gcgtagatat aagcctgggt acggatggtg taagagtctg
    16141 gctcaacaac ctgttctttc atatgggaaa cgacgcgata ggcaccacct gttccagtat
    16201 ttttcagcat catataacgc ttaccgccaa tctcttcgga ggcagctgaa ggaggatcct
    16261 gctcaagcgt tgtcagggaa actttcagtt tatggtccgg gttattgtca ccagaaatta
    16321 ctgcatccgc tgaagtactg ctctccgcat aaatatcatc cgggaaagtt acagcatagt
    16381 cgtggctctg cccatcagat gcgccaacaa tacctgttgc caaaggcgtc catgcagtta
    16441 acgtaccgct atatacccct ttcactggtt gaatggtatt attaacaacg ataggcttgg
    16501 tcaaagtaag attagctgac gcttcagtac cctggcttac taaagttccc gcctgagaag
    16561 caaaagctgt ccccgccagc atgattgcta acaatgtttt cttcattata ttttctccat
    16621 gattttcatt tacactgcaa aatgcagttt ctttctttgc atttacgtgc tatcgcgaaa
    16681 atgcttccac caaataatgg aaacatcttc acaaaaataa attatgagtt tcttaattta
    16741 gaaactataa acatgtcgcc gctatctggt ataaccctga tgcacttttc ttcgcaggaa
    16801 gttggtattt tgcgatacag gattgatctt tagaataacc ccatttcaca ttaaactcgc
    16861 cttccgcact caagccagaa acataagcaa taccatcttc acctacaatg ccggccatat
    16921 tttcagtgtc tttgctataa ctaatcacag cgccgaaggg gacgggttta ccctctttct
    16981 gttttaaggt tactaatcct ttatccccgc tcttcactgc caatgtcgca ggcacaacgg
    17041 ctccagctgt cggtacaacc aattgactag tttgtggtaa gtcgtaattc ttacctatcg
    17101 ttttttcatc cagaataaca tcgtctcttc ggtatggctg cactggcagt attgcatacc
    17161 cacgaaaatc agtactcagt cccggccagc cattaacgtt aacccccgtt gctccgggtg
    17221 cctctataag ggctaatgtc ccatccacat tttgaccaaa tgtaagtcca tcccttgtaa
    17281 taagtatccc accgcttaaa tttgcaccta gctgtttcat gttttcgctg gagctgtaat
    17341 ggctactaaa ctgaccatat gatccatttt tgccaacttg cacaatgttc atcgtgctct
    17401 tcggttgtga ctgatgagcg ttagttatat ttgtcgctac atcccaccat acaccaccac
    17461 tattctgacc tgataatgaa acctgatagt tattaccctg ctctcccgaa cgactcatct
    17521 gtgaagacgc ataaatttga ttttcgtcac ccagcgataa tggcactgaa atacccacgc
    17581 ttaatttctg atctactttc catttatgcg atgtcagcaa gtcgtttttt gcccagttaa
    17641 tggagaatgt tatgcctctc cataccgtac tataactcag gctggtagac cgcgtgtttc
    17701 ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt caaattcccg atattaccaa
    17761 ggggctggga taaggatatc gtagagccat cgtgactatt cttatctctt tttctgttcc
    17821 gttcatcctg tgcacttact gcttcagtca gtgtggcata acctgatgca taacggttcg
    17881 tggttataga c
//
LOCUS       DADLTI010000078        17463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLTI010000078 DADLTI010000000
VERSION     DADLTI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.078"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(404..601)
                     /locus_tag="JN755_002378"
     CDS             complement(404..601)
                     /locus_tag="JN755_002378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381144.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1447..2496
                     /locus_tag="JN755_002379"
     CDS             1447..2496
                     /locus_tag="JN755_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7381145.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            complement(3269..3703)
                     /locus_tag="JN755_002380"
     CDS             complement(3269..3703)
                     /locus_tag="JN755_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7381146.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(3675..4325)
                     /locus_tag="JN755_002381"
     CDS             complement(3675..4325)
                     /locus_tag="JN755_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381147.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            complement(4329..4913)
                     /locus_tag="JN755_002382"
     CDS             complement(4329..4913)
                     /locus_tag="JN755_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY7381148.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     VLMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(4904..5962)
                     /locus_tag="JN755_002383"
     CDS             complement(4904..5962)
                     /locus_tag="JN755_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7381149.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(5949..6374)
                     /locus_tag="JN755_002384"
     CDS             complement(5949..6374)
                     /locus_tag="JN755_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381150.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(6374..6922)
                     /locus_tag="JN755_002385"
     CDS             complement(6374..6922)
                     /locus_tag="JN755_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY7381151.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6922..8001)
                     /locus_tag="JN755_002386"
     CDS             complement(6922..8001)
                     /locus_tag="JN755_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY7381152.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            complement(7998..9326)
                     /locus_tag="JN755_002387"
     CDS             complement(7998..9326)
                     /locus_tag="JN755_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7381153.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(9387..11222)
                     /locus_tag="JN755_002388"
     CDS             complement(9387..11222)
                     /locus_tag="JN755_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7381154.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(11364..11633)
                     /locus_tag="JN755_002389"
     CDS             complement(11364..11633)
                     /locus_tag="JN755_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7381155.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(11633..11989)
                     /locus_tag="JN755_002390"
     CDS             complement(11633..11989)
                     /locus_tag="JN755_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381156.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11989..13482)
                     /locus_tag="JN755_002391"
     CDS             complement(11989..13482)
                     /locus_tag="JN755_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381157.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(13469..13636)
                     /locus_tag="JN755_002392"
     CDS             complement(13469..13636)
                     /locus_tag="JN755_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7381158.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            complement(13645..14205)
                     /locus_tag="JN755_002393"
     CDS             complement(13645..14205)
                     /locus_tag="JN755_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381159.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            complement(14202..14708)
                     /locus_tag="JN755_002394"
     CDS             complement(14202..14708)
                     /locus_tag="JN755_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381160.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(14683..15093)
                     /locus_tag="JN755_002395"
     CDS             complement(14683..15093)
                     /locus_tag="JN755_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7381161.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            complement(15090..15413)
                     /locus_tag="JN755_002396"
     CDS             complement(15090..15413)
                     /locus_tag="JN755_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7381162.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(15416..15616)
                     /locus_tag="JN755_002397"
     CDS             complement(15416..15616)
                     /locus_tag="JN755_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381163.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(15666..16871)
                     /locus_tag="JN755_002398"
     CDS             complement(15666..16871)
                     /locus_tag="JN755_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7381164.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(16886..>17463)
                     /locus_tag="JN755_002399"
     CDS             complement(16886..>17463)
                     /locus_tag="JN755_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7381165.1"
                     /translation="GSVFGVKDSHDDVVMSGAFAASLRAWSDRKALPALLWQHRMDEP
                     IGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSIGYVLKDWEYDRTK
                     EAFLLKEIDLWEVSLVTFPSNDEARISDVKNALARGEIPEQKKIERVLRDVGLSRTQA
                     KAFMAGGYIALSLRDAEDVGSALNALKNLNF"
BASE COUNT         4118 a         4870 c         4084 g         4391 t
ORIGIN      
        1 tatcatcatt caatgaacag atagctgtag tcaatgtatt attatgcacc acactgttaa
       61 ttttttgcaa aacaaacaat tacaatacaa aaccattatt aaatgcgatg gggatttcac
      121 tattagttat tatttatatt gcaacttgcc caggaaataa agtcaggtat tatgcagaga
      181 tagaacattg gtttaaagag tatggtaatt ttaatataat ccaaagatct atgcttggac
      241 ttaacttata tgcagatatg ttattttctg taaaatcaat tatcccagca ttactagctt
      301 tttccagtac tattatatgc aagaaaaaaa caaaaatact ccctctaatt tcttgctgta
      361 tcttgatcac actatatatt atacatacac cgccagttat ttttcaagcc atacattttt
      421 ctgaaagcaa tcttttctcg acactctccg tgtttagggt gtcttttgcc atgatactta
      481 cagcactcat tatatttcca accgtaatat cacttaattt taatgtcact tcaattttca
      541 tatccacaat gattattgga accattgcca caacatctat gttaggatta tcaccaagca
      601 tatatgcttc tggtaataga atttatttta ttccatattt gcttcttata acagcaattg
      661 ttgtttcaac cccaatcgca ataaataaca tagtagcaaa ttttgccaag agccattata
      721 aaatttgaat taaatttaaa agccacctcc tttttttggg gggggcttag aaataaaaac
      781 aaatttaatt ggacttttat tggttataac acattcctct atactgctca tcacccgact
      841 atactaggat tttcgtcatt ttttgctgaa ccgctctcat gaaatcctga caacaaagtc
      901 atgtttcacg tcaggatact tcttctggca tggaaatata ccgtgtgcag ctgatgcata
      961 tttatatttc aatataatac gatattgaat ggcctacacc actggtagtt caggccaaat
     1021 gacatccgac ggtttcacga tgaactacgg caaaagaata tcagagccct cattcctccc
     1081 cgaaagggag cgggctctgg tctggtgagt atgcagatcg taaaggctat ggccatggtg
     1141 cgagagtggc agctatgcca gaaagagtgt gtattgcctg agactctaac ctgatgcaga
     1201 gacgattgtc ttgaacctga tttattcaac aaagccgctg gcaggatgtt acgaaagtta
     1261 tcgcggccgc aggctgattt gatatcgata ctatcggtgg tactgcggaa gtaaatgcct
     1321 atatagtgcg actttgaaaa ataataaatt aatattgaac tcgttgatta ataatcataa
     1381 actttcaggc attcatattt gcaatgagtg tttgaaaatc acacaaaact ggagagccta
     1441 ctttaaatgt ataataacaa aatcgacaac aacagaaaaa caaggcatga ttgggttgat
     1501 gctttaaaat ttctcggtat ttttgctata tatttaggtc accttggttt gggggctggc
     1561 aaattgtatc cttttgtttt tagttatcac gtcccactat tctttttcgc tgctggtttt
     1621 tttactatca aaaaaaatga cttatctatt ttcgattata tcaagtcaaa gttttacaga
     1681 ttaatgattc catatttcac ttttgcattt actatattaa taataaacac aattaactca
     1741 ggtgagacga ttgattatat atatagccac atttatgata taatatatgg tgttagaaac
     1801 aaccaatttg taggaacaat atggtttata aactgccttt ttgttataat agcaattgat
     1861 gcaatcttta aagaaatagt caagaacaat atagtgattt taatcatatc actattatcg
     1921 ttcatgttgt cacaaacagt gctcaatcat aacccattac tagatcctca atggttttgg
     1981 aacatagact ccgctatggc atactggtgg ttattgcctc tcggtagatg tatgttttta
     2041 gaattaacac gtgacaggtt ttttggaaaa tctaaaattg gtttcattgt cttctcaatt
     2101 accgcgataa tgagtgcgta tcaactcctt aaccagaaac ctttgctttt taagattatc
     2161 tcaatattca atgcagacat aatatcaagt agttatattc aagctattaa tactattatc
     2221 accacggtag gcttgataat atttaatatt tttatagcaa aaataatttg tgggaatgac
     2281 tttattgtaa gagctggaag aaatactctt aacatatgtg gaatggagta tatcaccaaa
     2341 ttatttatcc ctatggcttt agcaatcata ggcttcagcg tgaccattcc aaatcctatc
     2401 tgcgcaatca tatatacctg tatttgtgta tatgtatcag ataaaattgg ccattggcta
     2461 tcgaggacag tcggtgggcc atttttgata aaatgataaa aaggcgcaat taattgcgcc
     2521 tttttataga tatatagcgc atatttatgc tcagattata ctgttaaaat taatgcgcaa
     2581 atgttacggt tttctttcct agcaactgaa gggaatgttc gaagtgcaaa cataaaatta
     2641 ccccttcact gaacagatat tcagtcggcc aacacatgag aaagaaatac accagatgaa
     2701 ttaaaatatc atatacttat taatcaaaga aataaaagca taaacagtag gtggtgacgc
     2761 tgataaactg gctataaaca acctggaagg tgatgagcta tcgcaatata atttattggt
     2821 gaattatttg aagccgattg ataacaccag tgctcaaaag actccccccg gtaaccataa
     2881 gtagctaata tctagtacag tttcgatatc tattatcgtt actgcgtcga tatagtctaa
     2941 atagctgcac ctaattctgt cacacttgtc gtttacagat gggtaatgtg ttgcgtatgg
     3001 gcaacaaaac tcaagccaga tataacacaa cctagctgag aacgccttcc tgatgcttcg
     3061 cccagcaagg gctgagcgaa agtggagcac ttttctgcgc agctatttag gacagtattg
     3121 agtcatattg tttatgtatg agttaagttt cgtaatatca attaaataaa ataagttgaa
     3181 atcattgtag aagtgagcat tatacccagg ctacctgaac ctactgaaaa atgagtatta
     3241 ttccaacaat tatcctagat taacaagtct atttatttgg atgcataggc cactcgggtt
     3301 ttactacatt aacacgcata agcagcaccc gatatttttt ccatgccagt aacagtttcg
     3361 tttcatcatc tgttgccata cctaaatcaa ccgcatcctg aagcggagct attacattcg
     3421 ttgcctctgc tatcagtgca gatttctttt catcatttat taaggctaat tgttctggtg
     3481 ttggagaagg acggtcaaca atgacaggat gaccgttctc accacaggta atcatttttg
     3541 ttacactctg tgcattaatc agtgttttcc actctgattc cgaaatttcc actgcatcat
     3601 caggaatatt ggtgccgtga aattcagtcg aataaaatcc gtttgtcgat gcagaataaa
     3661 aatatgacat tttgttaata tcccattgca atgtaatacg catcagcaga tgaaccgtat
     3721 cctgaaccgg gcgacacagt aatattaaat ccagtgttct ggacacttgt cgccgataca
     3781 gtcggctgcc cagccgcgcc agtaacgttc aatgtaacta cgacagcaat gactcttgag
     3841 aacgtcatcg ggaacctcat cccgtatgtt gaagatacat tcggggcacc attcaactga
     3901 ccgcactgta aaattaatcc agacggaaat ttttgcgcag cagccgttac ggtattaatt
     3961 gacgcgaaca ggctcatatc aggtatctga ttcgcccctg tacctacatt ccttttagct
     4021 gcttctccca aaccaaggtt ttcgagagcc gttttcaccg tgccatccga tttgatatcg
     4081 ccaaacggat tcttgcggct taacagcagc gcacgaagcg cggtaagcag ctggtcatgc
     4141 cgccccttct ccaggctggc accggatgcc tccaccacgc tgcaaagctc ctcctgcaac
     4201 atgtcaaagt agtcatcatc cagatcggtg gcaggtgtgc cggtctgggg gttaccacgg
     4261 gtaaaaccgt tcttacccgc gccgaactta tccttctgcg cggttttcgt gtctatacga
     4321 tgcatggatt actccggata tttaaaaatt acgtaggtat gcgacgggca gagtttgtta
     4381 agcacacact cgacaacggt gtcaccccag atacgcagcg cggaatcaca gggatcgcca
     4441 catgtcatcc aggtggtgtt ggtggcgact ggcatgttga cctgccagta ataccgccat
     4501 tccggcgcat tcaccgcgtc agtacaggca gatgagcagg tgaacgtgct tttgtcgtat
     4561 cgcgtgatgg tggcgtctgg tctgcccagg gcagcaagct gtgcaaggta aaaatcctca
     4621 ttgatgccgc ccgccaggtt aaccttcgca tccagtcgtt gctgacgctg gcgaagggtc
     4681 tgtgtccctg cgggaataca ttcatccggc agaccgcaca gacgctccca gcggtttatc
     4741 agttcagtgg tggtgcgcgg atccagctcc cgcatcagga catccgcacg ctgatgaacg
     4801 cgggttaatg acggtgccgc accggcaatc gccggatctc tggctgacca cgccggaccg
     4861 gggggcaaca gtgccgacaa cagacggatg taatcatcgt ttgtcacgtc catgaaatcg
     4921 tccccagaac cgccagttca ttttttgcaa tggagatatt gtcagccggg gcaagcaact
     4981 gatggctgta ttccccgttc gcaccggaaa tcgcctcact gatacgcgat accttcagtt
     5041 ctccctgcgg ataaccatca cgcagcagga acgaacgcaa ctccgcggtg atggcagccc
     5101 gtatttccgg tgtatccggc gtcacacgga tatgaaaatc caccgtatgc gccaccggcc
     5161 tgaacacata caaatcagag cctgccaccg gggccagcgg cccgatatgt tgtcttgccg
     5221 ccgtttccgt tgattcttcc ggaatgggat taatcaggtc actactggca atcatcacac
     5281 cgacagtccc cgttcccatc cagtgacggt atgcccatgc gcgggtaatg ccgggcactt
     5341 ctttagccca gacgacatag tccccgtcag ccccgccctg cggcgtccag taataccgct
     5401 caatgacgcg ggcgcgccac gtttccagat cttcagtatc aaatccacct gtcagggtat
     5461 ctgccacacc ggaagacggc agaccattca ccggcgtgac caggattaat gccgtaccgt
     5521 cgtcagcgtt accgaccgcg cctgcagttg aacaggcgat cggcaggcgc aggacaccac
     5581 cggagctggt tgcatcggca gttgccgtgt actgaaccag gtcatcgcgc tgaataacac
     5641 ttccggcggt caccttcagg ccatcgctga caccttccca gcgcatatac ccgctggcag
     5701 ccgtggcccc cttgcgcgga caccgtttca tcgcagcatg tcgcgccagc cagaactcat
     5761 cgcacaggtc aggcagcatg ttcattgcca gataatcgat gtacccgtaa accgtatgca
     5821 gcgccgccgc atacaccttt gcccgcacgt cttcatccat gcgccggagc gtgtcgctga
     5881 cgtccagcct ggcgaataaa tcgttacgga gcatactgat attttctgcc agcgtcgggc
     5941 gctgaaattc actgtccgcc atgcgttatc gcactccaca gatcatcaaa agaaatcatt
     6001 accggtccgt cacgacgcca gagagtgata ctgttaccca gttcattaat cccggtgcgg
     6061 cggatatcca gatcaatacg ggacaccacg ccgtcatcag tcatccattg caggcattcg
     6121 cggatatacc cccttaccgt ctgcaccagc tgattggtca gtttgctgcg ctgaagcagc
     6181 cacagtctgg aggcgtaacg gtcattctgt accgcaggcc aggtatcccc ccaccatccc
     6241 atcgggacgt cggcgttgtc atcaggctcc gcccgccgcc aggtaaacag ggaaatcacc
     6301 acggcgcggg tcagcggatc cagcggtgcg ctggcgcagg tgcgtttacc gttcaccgtc
     6361 agccacagtt ccatcatgcc tccatagctt tatcaggttt atcggtgtta ctgccctgac
     6421 cgttctctct gtgacgatga ccgttatagg caagccgcat tgctgacatg gtggtgccgc
     6481 cggagtcgca caggtctttc acctgtcctg ttacttccag gtccatttca aaacgtgctt
     6541 caggtgcatt gcgaaacgtg atcgttttac ctgcaccgtc caccacgatc ccctcccggg
     6601 tcagcgtcac ggactgcccc tgatcgtcat agacagccac ctcacccgtc tgcagccctt
     6661 tcaggcggta gcgccggtct gacaccgtaa caaccaccgc atgagaacgg tcgccatccg
     6721 gaaacaacac caccgcttcc gcaccgctgt ttgcccttgc ggtaaaaccg tagggttcaa
     6781 gatgttcaac cccggctttg ggttcaccgg caatcaggga cacatccacg gtcagacatt
     6841 tcgtggcggc actgatgctt ttcaccacgg cccgcccaat caggccgagg agttgtcgct
     6901 gcatggcttc aatcgtcctc atcagaacgg gtcctcctgt actctggctt ttttcttttt
     6961 ccgcgcgccg gggtcttcgg gttcaggcag ataagcatca ggcgggccga cacggatttc
     7021 cgtcagagtg ccattctggt cctgagtaaa cgtgacttcc gagacaagca gttcggtatt
     7081 gtcaaaacca cagaccggat cgaagacaat cacccgctgg ttgggctgcc acagcgtacc
     7141 gttaccctgt cgccagccct gcaccacata ggtggtttca tccgtccgcg ccgcccgttg
     7201 ccgggcttca aagtcagcac gcgcaataca gcctgccccc gtggcctgcc ctgtctgcct
     7261 gatatacatc ggacggtaac gggcaataaa tgcgtcctct gtgcgggccc gcagcgcggt
     7321 ggtggtggcc tcaccgaaat catcgtcgtt tccggcacgc tgccccgcca cctggtaaac
     7381 agaaaaccgc tcccggatac tcttctccgt atcgcaggaa aggatgtttt ccccgagtac
     7441 cagcgcagta tgtgcccgcg ttgagccaat accaccaatc accagcctgc cgtgcgggtc
     7501 gtcgtaagcc agtgcctgct gctgaccgag tattttgttg attacctcaa tcaccgtttc
     7561 accgtgatcg ggctggacgt caggaataac acccgacggc gcaccgttgt tcaccacctc
     7621 aatgccgaaa ggcgcagcaa gcgcctgcgc aatctgtacc agctctcgtc cgttaaactg
     7681 tgtcggttcg gctgcacagt caatcaggtc agccgtcaga ctgcgtccgg caataccggt
     7741 gctgaccgaa cgggcatcgt aacgaacggg cgtcgcctcc acccagccgg tgatcaccag
     7801 ctcatcacca atcagcactt ccacttttga accgttttta atgcgcggct gaagcgtggt
     7861 gataccccca tctcccggcc actggcggga gatctccaca ctgaaatccc gcgccagccg
     7921 ttcaataccg gcaccgatgc gcaccgatgt ccagccattc cactcccggc catttacccg
     7981 tagcgtgacg ttatcgttca ttgcactggc accttcagag ggatcaccgg cacaaagccg
     8041 ggatgcgtaa tggcattacg ccggataatg tccgcgtcac gcgccgcgtt atcaaaccag
     8101 gtcgccgcca gcaccagcgc gggtaaaacc tcatccggtg tgcgctgaat gatccgtgca
     8161 gactgttcaa ggcgcgtgtt gatatccgca ttcagatctg ctttcacccg gcgcagcgcc
     8221 agaaacagcg catcactggt tgtacgggac aactccttat caattgccgt attcagtgtg
     8281 tcgcgaatgt cagtcagttc ttcccacgtc ggcaggtcaa ccgtgttttt caccgccggt
     8341 gcattgttca gtgccggatg cgtgacggaa ggccagccag tgctctgcgc aggtgttgtt
     8401 gcctgctcca ctgcggcatt ctgcatcacc gcggaagttg ttggcgcagg caatcgggtg
     8461 acggcatacg ccgcttcgct gattgcggtc gtacgaaggg tgctggcaac cacgttacgc
     8521 tgctgcgtcg ccgtggcggt ggttttactg tccgttttcc agacgccgcg cggttgcaga
     8581 tcgctgccga ggctgacacc ggaaagcgtt ttgatcatgg tgaccaggtc gctggcgtta
     8641 ccataaaggc gtttcccggt acgccacatt ttctgcacct gctcaacgaa atttttgcct
     8701 gacgatggcg gcggcagaag taccgagata tccccctgca acagcctggc gacatccgat
     8761 acggcagaat ccaccacttt catcgcatca gaaacatacc caagcattgt gctggcatta
     8821 ccgacgacgt cgttctgcac aaaatctgcc acgccatcga tactgaaacc actgaaactg
     8881 tcactgatgc agtcatccag tgcagaacag gatgacatca gcgtctgcgc cgtcgccgca
     8941 cctgatgtgg ggtaagagag ttctcccgct tcgacaaact tcaggtcaaa gcggacaata
     9001 cgcccttcac ccttcgatgt gctgacccga acctccccgt caacacagac tttcagctca
     9061 ccgtatgtcg gatggacaag cgtgccggga ccgggtttat tcagcgcgtc aatcaggcga
     9121 tcgcgctggt caaagcagtc atctcccacc acataagccg tgatggacgg gcggaaagtg
     9181 acttttccca gatcttcggt atagggtttg tcgcggttcg ggtattcgtg cgtttccaca
     9241 cgacgaccgg ttcccgcatt ttcttcttca accttaaacg gcacaccgcg aaatgacgcg
     9301 tcctgaagtc tgtctttcca cgtcatataa actccgtaca taaaaaatcc caccggagtg
     9361 ggactcatta acagattaat ttttcattac ctgccaaagc gcgtatagcc aacatcatgg
     9421 ctgacatcaa aaccgctgga tcgcgtttcc ataacccgca tacccggagg cgaattcaca
     9481 aaagagacct tgatctcacc atcaactttt ggcgcagaag ctttgttaat catgaaggga
     9541 ttcgggcctg tggcatcgga ggcgttgttt gactgagcca gatccaccgc cggataaggt
     9601 atgtatcccc gcgccggtat tcccgtccca taagcatcat aagcacccgc gccccactgc
     9661 gccgagttaa tggcatcgac cgtgtcacct gaactgtcgg taaaccactc aataattggc
     9721 ttcagcttat cccacatatc ctgaaaccac ttaacaaccg gtccccagtt attgatcacc
     9781 atccccagcg gcgaccaggc aaaaaccttc ttcagaagtt cccagccagc ctcaaaataa
     9841 ggactaatgg tttcccagag tttcttaaaa taaggtccga caacatccca gttagtgata
     9901 attaatcccg cagccagggc aatcgccgtc gcaatcatgc caatcggcgt catcgacatg
     9961 atcctgctga cgatactgat ggcactgccc acgcccatca atcccagttt cagaatcgca
    10021 agaccggcag caagcccgac gacgccgcga ataacccggg gattttcatc cgcaaacttc
    10081 gtgaatttct cccccaactc ccccagccat tgcgtgatat ttttggcgtc accagaaaat
    10141 gcgccgccaa tagccgcaag gccgttagtt gcggtccccg tcattgcctc ccacaggttg
    10201 gacagcgtac caagctgggc ctgaacacgt ttattcaggc tggcctgttt attcatcttc
    10261 tgctggatct gatcgtagcc atcctttcct ttatcgatta gtgcattgac cacctgaagg
    10321 gtttcggcat catcaccaaa tattgcctta agtacacctg ttcgcttaac gtcggtcagt
    10381 tttcgcagct ttgccagttg cctgaacatg ttatcaagac cgccaaaact tcctttgccg
    10441 tcagtaaaat cgagctgtac cccgagtttc tggcgggcca tgattttatt gacgtccctg
    10501 attttcttaa cgcttaatcc ggactggata acttttcgca gggcattacc tgccgactcc
    10561 ccgttcatcc ccatctgatc catcatgacg ctgatggggg caaggctctg tgcagcctga
    10621 agaccgtcct tgttcaccat cttcagaaca gaactggttt tagtgaagaa ggacaacatg
    10681 ttggtatcgt caacgcccag ataaaacgcc ttctggatag tgtcgaacag ccccatcatg
    10741 tcttctgacg ccgttccggt agcatcctgc atctttgcag caaactcagc agccgcttcc
    10801 ggtgtttttt tcagttgtac cgcaagataa gctgtcgctt tacccacacc gccaagaata
    10861 ttttctgccg ggatcccctg acgcaccagc atctgcatca tgttctggaa atcagccgtt
    10921 gtaccgggta gctggttacc caggccaata gccagtttat tgatgtcctg aaagctcttt
    10981 ccaacctcgc cattcgcatc catcatggcg actttcagcc cggtggcggc gttttcctga
    11041 tcggcataag atttcaggga aagcgtcaga cccgctgcca gtccgccacc aagcgccagc
    11101 ccaccctgtg acgcttcttc cgcctggcgt ttaaatcccc ggattttctt ttgcattttc
    11161 gacagcgcgg gagaaagcct gtcgacaccg gtgatcaacg ccttaagctc aaattcagcc
    11221 atgtgtgcgt ttctcctgct ctatcctgtt tgcctgactg accagcaagg gaatttcact
    11281 gatcggcata ttcagcaatt cgaagggatt aatgcgccag tagctggcgc aatcaaagaa
    11341 gcgatcagta aggtattcag ccgtcaggcc tggaggaaaa aaccagccac aagccacgcc
    11401 gctgcattca ggtctgccgg agacatctgg tcgacagagc tttgcggcac tttcgccagc
    11461 cgcacaatat atttcgatac cacatgcgcc agaagtctga cggactcatc ctgattcatc
    11521 tggtagggat accccagctc gcggacatct ttcccggtgg gctcatcaaa ctccagtacg
    11581 gagagtgtct cgccatgagc ggtaatcggt ttctttaact caagctcttt cattactggt
    11641 aatccccttc ttcaccgtgg aactcaagat cgaccgtgcc ttcttcggca ttatggttcg
    11701 cttcgccgtg cagccaggca gacgacagta catagacctg accgttcgcc agctcggcag
    11761 tgatggtcat ctcatcagac gaggtgattt tgctcgccgg aaaattcttc ggcaccttga
    11821 aggtcccttt gacataaggc gcacggtgag tttccttgcg gtccactgaa ccgtccaggc
    11881 cgatgatgtc atcattgacc gtcctgttca tgggcacctc aatgccgccg gtcagcgata
    11941 gctgttgacc gtcaattttg aaataacagg ttcccccgat acgggccatt atgcagactc
    12001 ctctgaatac tgaagacgga actggttaac cacggcaaag acacgcaact ggttaacata
    12061 gtcaggcggg aacagcgtgt tcaggcggtt cggatcgctg gcatcacgct ccacaaccag
    12121 gtactgctta aacagttcgt agttttccac gatccccgca cgctcaagct gacggtaggt
    12181 tgccagcagt tcccctttga tcaccgccgg tgtgacaatc gcctgaccgg gaccaaagcg
    12241 ggtaccgtcg ctggcaagct tgtgacgccc gtacttactg gtaatgacgg atttcagttt
    12301 gcgcagcaca tacgcgctgg tatgcagcgt ctcgctgtcg aggtagctgt tatccgcaac
    12361 accgtaagcg tttttcctgt acgtggtgac atcacgctga atgcgcagca ccccgctttc
    12421 gacatacgcc gttgccacgc catgagacag cagggtctgt tgttcggtca tcgtgaaccg
    12481 tttccccttc ggcgcaggca gcatacccac cagctcaccg gtctgcgtgg gacgtgccgg
    12541 atcgttgcga ataaacaccg ctgcgcgggc ggtacggctt gccgccagct cgtcggcagg
    12601 cgtctgggtc tctttttcgt atcccgccag ggtaatgtgc tgctggttaa actggtcacc
    12661 tgcggtcacc agttctgaca gcgtgccgat ctttgccgta tacacatgac catacagctg
    12721 acgcgcatag ctccagcgac cgctggtatc gttcatctcg gtcaccagcg tgttaacgga
    12781 ggccgtgtcg ttgaacggca ggccgatata atcaaacggc tcatccgcca ttgcagccac
    12841 cgcgccggtg agaaccggag cacccgttcc ggcggtaccc gtcgccacgg caatctgtac
    12901 gcccgctggc agcacttcgc ccccaccaaa gccgtagtaa ttgaggctga caggaatttc
    12961 attcccgcaa agccccttat gacgcgcggt cagtgtgacc acgcctgccg aagatgaagc
    13021 cgtaaacggc agggccggaa cggcattgat ggcatcctgg atactgctgg caatcgtcgt
    13081 gacgttatcg ccgttggtca ccggagcctg cacgcgggta cgtcccacat agacattcac
    13141 cgtgccgctt tcggttgctt ccccggtcac cgtcagcgta accgttgccg ccgcgcctgt
    13201 ggcttctgga acggcaatca catacagctc accaaacggg tcggtctggc gataagcctc
    13261 gaccatacgc gccagctgac ttcccgcacc acaaatctgg cgtgcatagt ctgccgatgg
    13321 catcagcacc agactgttgg caacaatctc tgcaccgtta ttggcatgac caatcagcag
    13381 cgatgctccg ctgtcctgtg cagtattcgc cgccgagtta tccatttccg cataaaaaat
    13441 cggaaccagc gtatttgacg gaatggtgtt aaagctcgtc atcggtattc accttttcat
    13501 tcacgcgccg gatatcaccc gctgcttcac ggcgcagcca gtagttgttc tcatcaacat
    13561 ttcgcccctc ggcgggcaaa aggtctccgc gggcagggtc aggaactgac cgccctttaa
    13621 caggtttcac aaacatgaag attctcagga aggaagggtt atttcggtgt gatgttcgat
    13681 atcgccgtca ggcccgttac cgggctctag ataatcaaca tcaatcgcca gcgttcgcag
    13741 ttcatccaga ctgttcaggt catcctgctg gcgggtatcg tcttcggtca actcgctgat
    13801 gaccgaaaaa tcgaactgat aaatcagctc atgacgattc agatccagca gcgtgccgcc
    13861 gtcataggta atcgggttac cgcacgcttc cgggttccag cccagcaggg ccttaaagag
    13921 catttgccgg acatcgtcca ccacatcata cgaggcaaac tgaccgcgct catcacgccc
    13981 gttactcagt atgacaacca cggagaagcc ctctttcagc tcctgccagt agtcggtctg
    14041 gcttttgttt tctcccggag agtcatcacc cggtaccaca tacgccgccg ggagtctcag
    14101 ctttccgacc tccggcagat ttttgaactg tgccgcgcct gccacccggt tttcaaaata
    14161 cgggcagcgg gcacgcagcg cagcaataac aggcgtcagt ttcatctgtg tcgtcgctcc
    14221 ggcttcagtg atttacgcaa ttcccgcgcc agaaaatagc gtgtccagct gcggttcttt
    14281 tcaagcgttt ccaccataaa gttattacgt ggagccagtc gccagccgct gccaccggat
    14341 gcaccacgat gatggctgcg acgacgcttt gcccctcgcc tcacgccata gaacaaaaaa
    14401 gccggataaa aatcaccggt gatacggcgg tttccctctc cattacgctg gttaggggct
    14461 atacgtgcca taaaaccagg gcgatgttta ctggctctgg gtaccatgta accaatcgaa
    14521 cgagccaggc gtccggtctg ataaccgggg ttttcacccg gtgccgaccg cgcacggcgc
    14581 atcaccagcc gacgggcatc acgcatatga cgctgaccaa tcgtgacaaa cgcccgccgg
    14641 acacgggcgc ggttaaagcg catctccgcg ggctgctgaa aatcaacgtg caaaaaggaa
    14701 gtcgtcattg ttgcctccgt gactctgccc acattcgccc agctccgtac actccagcag
    14761 cagaaagcgc cgcgccccgt tcagatcgcg ctgacgtttc acccggtaca cactgtcacc
    14821 gcagaccacc tcataatcag cggtaatccc ccggcggtaa cgaatggtga tgtaatgggt
    14881 gatggcgtcc ccggtctgcg cggtttcctg ccaggtggtg gcactggtct ggataacctt
    14941 cgcccatgtc cggaacgtaa tcaggtattg aggctccacg ccaaagttat ccgcgggcat
    15001 atccacccgc aggcggatca ggacgcgttt attcagttca ccggggtccg gcagaatgta
    15061 ggttgcgctg gtctgcgcct gacgaatttt cattgcggaa agtacctgta cgggccgaca
    15121 agccagccaa aactctgcgg catgtcaagt ttctccactt ccgtaaccga cgagcggttt
    15181 tcgtaaaaat ggctgataag catcagcatc cccagacgaa tatcatccgg caggtgcagc
    15241 ccgtccggat cgctgtccgg aatggtttca tccggtgcat agagcttccg gttcagatac
    15301 gtttccgtcc gcttttgtgc cgcacaggcc agcagttgca gatggcggtc atcagcatcg
    15361 aaatcctcat ccagccggag ttgggcttta atctcttcca ttgtcagaag catactcagc
    15421 cctctttact ggtcgtggct tttttctctt ttgtcgcttt actgcttttt gcactggttc
    15481 cgcgctctgc taacccggcc tgaagtgcaa tctcctgcac ccgggcagga agcgccccgt
    15541 cgtcatactc accggcccga atgacctcaa cacgcatacc gtccggtgac catttcagat
    15601 cttgtttcag gatcatgatt cttcacccgt cagaacaggg ggcgcggttc cgcgcccctg
    15661 agtgattacg ccgctgcaat cttcagcagt ttgatggcct gcgaatcgac cagcatcccg
    15721 ccggtgcgct tggtggtata aaaaccgaca aacggtttat tggtgtacgg gtcacgcaga
    15781 atgcgggtgc cgatacggtc aacgatggtg taaccccgtt tgaagttacc aaatgcaatg
    15841 gctttcgcat cagcggcgat atccggcatc tgttcgtttt cagcgatacc gtaacccgcc
    15901 agagaggacg gctgccccag ttccagcccc ggacgccaca gatagttacc ctcgctgtct
    15961 ttaagcagac ggatggcaaa caggctgttg ttgttcatca tgaacttcgc gccggtgcgg
    16021 tgtgccttac gcagcgtgta aataagtttg ataatggcgt ctgcggtcac cgccgtcgct
    16081 tcgccggata caatatgctg aagtttgccg aacgcccgga ccttatcggt ttcatcagtg
    16141 gattcatacg ccaggaaccc tttcggcttc ttggtaccat cgccggtggt aaaggcaatt
    16201 tcttcctgtt cggcaaattc ggttgccagc tcgctgttga tccaggcctc cacgttgaag
    16261 aaggcatcgt ccagcatttt ctgggtggcc tgcgggttgc cgtagatttc ccccatgaga
    16321 ggttcaatca gctccagtct ggaggtggca gtctgggatc gcgtatccgt ttcccccacc
    16381 catccggaag ccgtgccgcc cagattcacc agttttttgt agtcggaacc gccaacggtg
    16441 atcaccgtgg cttcctggcg catcaccact tcatctttca gcagggtgag aatgttgcga
    16501 tccagcgctt ccggcacggc atagccaccg tcttcatcgg tgcccacctg caatgcctta
    16561 cgctccagat cgcgcagacc atcttcacgg cctttacgca aaaagcccac aaacgcttct
    16621 ttatgctcgg tggccagttt attttgcgcg ccacctgccg gacgtttcag ctcaagcagc
    16681 tctttttcaa gatcgctttt gagattttcc agctcgctga gtttcccgtt cagggtttcc
    16741 acctgcccgg caagcttgcc tttttcctgc tcaatcgcat ccacgcgctt gtcgttcttt
    16801 gctttgaagt cgtcaaactt ctgctgcagc tcctgcgcga cctgttcgac atctttaata
    16861 tctaccgcca tcgtatttct cctgattaga agttcagatt tttcagtgca ttcagtgcag
    16921 agcccacatc ctcagcgtcg cgcagggaca gtgcgatata gcccccggcc atgaatgctt
    16981 tggcttgggt acgggagagt ccgacatcac gcaggactct ttcgattttt ttctgttcgg
    17041 ggatttcccc gcgggccagc gcgttcttga cgtcgctgat ccgcgcctcg tcgttagacg
    17101 gaaacgtcac cagactgact tcccagaggt cgatttcttt cagcagaaag gcttctttcg
    17161 tccggtcgta ttcccagtcc ttcaggacgt acccaataga aaggccggtt aacgaaccgg
    17221 ccttcatgtg tgcatgtgcg cgttttgcca ggggatcatc atcaatgagc aaccgccccc
    17281 tgacgtaaag cccgacatcg tcttccttca tttcggtgta aacaccgatg ggctcatcca
    17341 tgcggtgctg ccagagcagc gcaggtaacg cttttctgtc actccacgcc cgcagggaag
    17401 cggcaaatgc cccggacatc accacatcat cgtggctgtc ctttacacca aagacggagc
    17461 cat
//
LOCUS       DADLTI010000079        17458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLTI010000079 DADLTI010000000
VERSION     DADLTI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.079"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            564..1334
                     /locus_tag="JN755_002400"
     CDS             564..1334
                     /locus_tag="JN755_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7381166.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JN755_002401"
     CDS             1325..1798
                     /locus_tag="JN755_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7381167.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JN755_002402"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JN755_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7381168.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JN755_002403"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JN755_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7381169.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JN755_002404"
     CDS             3233..3727
                     /locus_tag="JN755_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7381170.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JN755_002405"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JN755_002405"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7381171.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JN755_002406"
     CDS             complement(6410..7432)
                     /locus_tag="JN755_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7381172.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="JN755_002407"
     CDS             7571..8485
                     /locus_tag="JN755_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7381173.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="JN755_002408"
     CDS             8699..10060
                     /locus_tag="JN755_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381174.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JN755_002409"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JN755_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7381175.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JN755_002410"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JN755_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7381176.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JN755_002411"
     CDS             12610..13899
                     /locus_tag="JN755_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7381177.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JN755_002412"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JN755_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7381178.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JN755_002413"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JN755_002413"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7381179.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JN755_002414"
     CDS             16225..16605
                     /locus_tag="JN755_002414"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7381180.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="JN755_002415"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="JN755_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7381181.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4293 a         4626 c         4375 g         4164 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgttgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DADLTI010000080        17407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLTI010000080 DADLTI010000000
VERSION     DADLTI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.080"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JN755_002416"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JN755_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7381182.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3184)
                     /gene="hemX"
                     /locus_tag="JN755_002417"
     CDS             complement(1967..3184)
                     /gene="hemX"
                     /locus_tag="JN755_002417"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7381183.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3206..3946)
                     /gene="hemD"
                     /locus_tag="JN755_002418"
     CDS             complement(3206..3946)
                     /gene="hemD"
                     /locus_tag="JN755_002418"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7381184.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3943..4884)
                     /gene="hemC"
                     /locus_tag="JN755_002419"
     CDS             complement(3943..4884)
                     /gene="hemC"
                     /locus_tag="JN755_002419"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7381185.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5271..7817
                     /gene="cyaA"
                     /locus_tag="JN755_002420"
     CDS             5271..7817
                     /gene="cyaA"
                     /locus_tag="JN755_002420"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7381186.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7857..8177)
                     /gene="cyaY"
                     /locus_tag="JN755_002421"
     CDS             complement(7857..8177)
                     /gene="cyaY"
                     /locus_tag="JN755_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7381187.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8225..9538)
                     /locus_tag="JN755_002422"
     CDS             complement(8225..9538)
                     /locus_tag="JN755_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7381188.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9535..9840)
                     /locus_tag="JN755_002423"
     CDS             complement(9535..9840)
                     /locus_tag="JN755_002423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381189.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9862..11013)
                     /locus_tag="JN755_002424"
                     /pseudo
     CDS             complement(9862..11013)
                     /locus_tag="JN755_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11202..11405
                     /locus_tag="JN755_002425"
     CDS             11202..11405
                     /locus_tag="JN755_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7381190.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11442..12266
                     /gene="dapF"
                     /locus_tag="JN755_002426"
     CDS             11442..12266
                     /gene="dapF"
                     /locus_tag="JN755_002426"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7381191.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12263..12970
                     /locus_tag="JN755_002427"
     CDS             12263..12970
                     /locus_tag="JN755_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7381192.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12967..13863
                     /gene="xerC"
                     /locus_tag="JN755_002428"
     CDS             12967..13863
                     /gene="xerC"
                     /locus_tag="JN755_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7381193.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13863..14579
                     /gene="yigB"
                     /locus_tag="JN755_002429"
     CDS             13863..14579
                     /gene="yigB"
                     /locus_tag="JN755_002429"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7381194.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14663..16825
                     /gene="uvrD"
                     /locus_tag="JN755_002430"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14663..16825
                     /gene="uvrD"
                     /locus_tag="JN755_002430"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7381195.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16972..17364)
                     /locus_tag="JN755_002431"
     CDS             complement(16972..17364)
                     /locus_tag="JN755_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381196.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4059 a         4483 c         4610 g         4255 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac
     2101 gcacgcgagt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     2161 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     2221 catcatcagt atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat
     2281 aagtctcttc ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     2341 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     2401 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     2461 attcgctgat ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt
     2521 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     2581 tgccgtcata atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc
     2641 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca
     2701 gcaacgctgc agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     2761 ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     2821 tcttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct
     2881 taagctgcgc agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg
     2941 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     3001 cctgttgttt accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca
     3061 ccgcgctgag aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg
     3121 acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg
     3181 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag
     3241 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     3301 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     3361 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg
     3421 attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     3481 cacaaaaagt gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc
     3541 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     3601 gcaagacttc gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat
     3661 gtagcgccag tgcggtggtg cgtccaatgg tgaaataagt aggtagttgg ggccatttac
     3721 gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     3781 acagatcgct ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg
     3841 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacgtctca
     3901 ctaactcttc tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc
     3961 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     4021 aatccccatt tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     4081 accgtccggc gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta
     4141 gctaccaatt ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt
     4201 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc
     4261 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     4321 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     4381 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     4441 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     4501 ggacgtcccg acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac
     4561 aaaggcatcg cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa
     4621 ttcaaccggc acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc
     4681 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     4741 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc
     4801 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     4861 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     4921 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     4981 aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac
     5041 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     5101 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     5161 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     5221 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     5281 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     5341 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     5401 atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     5461 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     5521 cagtacagga tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     5581 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     5641 ggctcgatag cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg
     5701 ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata
     5761 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     5821 acgaatttta tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     5881 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     5941 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     6001 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga
     6061 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     6121 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca
     6181 tgatgctgga gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt
     6241 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg
     6301 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     6361 gtctggcaat gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca
     6421 acgagctgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     6481 ataaccttag cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg
     6541 ccgcgtttga agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     6601 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt
     6661 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     6721 ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc
     6781 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     6841 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     6901 acagcccgtg tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag
     6961 ctgcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     7021 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     7081 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     7141 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     7201 agcatctgcg cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     7261 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     7321 aaacctgggg cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca
     7381 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa
     7441 ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt
     7501 tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca
     7561 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca
     7621 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     7681 acctgccgca gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc
     7741 gaacaaaatc gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac
     7801 agcagtattt ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag
     7861 cggaaactga ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag
     7921 gtttcgccgc tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc
     7981 tgtttggttg ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg
     8041 ccgttttcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg
     8101 ccatccctgt cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggcgatga
     8161 aattcactgt cgttcattgt tgtaccctgt ttttaagtga tggcggcagt atagcggcat
     8221 ggggtcaggg cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca
     8281 ccggaataat gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt
     8341 tcgccgggca gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc
     8401 gatcgtcata agcgaagcga tcgtttttag ttagcggacc gttaactgga ataatttgca
     8461 agctctttga ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc
     8521 gatcaatgac caagtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg
     8581 tcgataattt tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat
     8641 ggaattgttc aggttcaata cctggtaggt ctatccactt gctccactga cggtcgggcg
     8701 tgagctgttt acgccagcga acgcgacttt cccctacttc aaaagagacg tcatcatcta
     8761 cgggtatcgc tttgcctgat aattgaccaa agggataacg atgcagaccc ttattgtcgc
     8821 gaaaaaccag cagtgaatga ggtatccagc gaattatctc aaatgagtct gcattcgccg
     8881 taaggtgaac gccatttaaa taaatattct gcccactgcg gataagcaga tcattggaat
     8941 atgaatttct gtttacactc ataagacgtg gattaatacc ccaccatctt ttttctaata
     9001 cagtaacatc actgctgctc ccctgacgtc gaccggacaa gtagagattt ttactatcca
     9061 ccagcgtggt agagttacct tcgatagctt ttagtgtcgc cagatcaaca cgtgacgcgc
     9121 cgctattact ttcggtgcgc tgtccgtcaa aatagaggct atatttatcg accgcgaagc
     9181 ggcccatagc ctgaaagctg gcaatatcga cagtgggtgt tcccggtgga ttgctaacga
     9241 ttttgccacg ccagagaata acgcggccgt ctgtcaccca gtggtattcc tcattttcta
     9301 tgcttatttc atcggaaggt atataacccg ttttcccaga gatatttttc acaaggcgca
     9361 aaccggccct gctcatctcc ggaaactggg caatgatttt cttatccccc caataacctt
     9421 taccgtaaga cataacaccg tgatcattgt aatagtcgaa ccctatggcc tgtacagcag
     9481 gcgtcagaaa tgtgtgtccc aacaggatga gtgcaactgt gattatttga cccttcattt
     9541 tgtcacctca ttgcgcgggg caggaataaa ttcgccgtca tcagtaattt ttccgtccag
     9601 atatcccgtt ttgtaattta cgccatagac atatcccgaa tgttgttcct tccagattaa
     9661 gcgaacttgt ttcggatcag cggtgcggta tcgccagagc tgcgcgccat caaagctgta
     9721 aatgtagcgg ttatcgcggg cataccatct gcgcaaaaag ggatggtcat taagatgggc
     9781 ttcgcgttca tccggagaac ggttatccag taagatcagc ggtccgtcag attcaaacac
     9841 gtgtaaaccg gaatggtatg gctacccttc ggcacgcgta agtaaatagc cgtggcgttt
     9901 cccggcgttg aaacgtttgt tgataataag attgggatca tcaagtttca cgatctccag
     9961 cttacggtca ccagactctg tttcgatgat ggtataaagc gagtcctgat actgggcgac
    10021 agcatcactg atgggttgaa actgctccgg atcgagtccg ggtaattctt cctgtttaca
    10081 agcaggtcct ttacgccaga acactttctt ttccagcaga gaaaaatcca gacagtgctt
    10141 tgttaaatct tcatcccacg ccagattttc cttgtcgagg actttacgct gcagcccgtt
    10201 tttatcacgc caggtcaaga gtgagccagg catccagcgc acgacggaaa aggtgtccgg
    10261 gtcggcatca agctgttcgc cgttgagcag gattttggtc cgtgtacgag ccagggtatc
    10321 ccaggggcca aatggtacgg caatgcaggg gttaaacgtg gtggaaaact ttcctcgctg
    10381 atcccaggac ttctgcgcca gcacacggaa actctcgggg tcagcgaggc gatgcccatt
    10441 gatatacaag aaattagcgt cacgtaatat cagccccagt aagtcaggat cccagggagg
    10501 ccggaattcc acctgatgca gcgttttgat atccaagcta tttccaccac cattgtcatc
    10561 ggtgcgcttg ccttcaaaat agagactgtg tttatctgcg gcaaaatcac cccatgcctt
    10621 gaacgaggcg acatcgacgg gaggtgtgcc gggagggttt tgcacaactt cacccgcgta
    10681 gaggatattg cgaccatcgc tatgccatga ataatgagga tactaggcct gtgcgccctc
    10741 atagaggtag tcggttatca aatcagtcag aggcggttct ttatcccatt cgtctagtgt
    10801 tgtcgggtct attaataaat tgggtaaccg acgcaaactc ttgtaattca cgtcaggtag
    10861 agaaagattt gtttctttat aagggcgcag gtacaccacg ccttttccct gtttctggta
    10921 aatattatgt tcaggtgcgg aaagccgaca tccccatgcc gacgataaaa acaatgaaca
    10981 agccaacaaa caaaccacat tgcgatagtg cataaagcca tcctggcgcg aggtgttgat
    11041 cacgaaactg ccagcagaac ataaatcctc acgagtaagc gttatactcg cagcatttcc
    11101 tcacttttca gacttcataa agagtcgcta aacgcttgct tttacgcctt ctcctgcgat
    11161 gatagaaagc agaaagcgat gaactttaca ggcaatccat aatgaaaaac gtgtttaagg
    11221 cactcactgt attacttact ctcttcagcc tgacgggctg cggtctgaaa ggtccgctct
    11281 atttcccgcc tgcagataaa aacgcaccgc cgccgaccaa accggtagag acgcaaacgc
    11341 aatccacggt gccggataaa aacgatcgcg ccactggcga tggtccatcc caggtgaatt
    11401 actaaaagtc agtttctgta cccgcgtgat tggagtaaat gatgcagttc tcgaaaatgc
    11461 atggccttgg caacgatttt atggtcgtcg acgcggtaac gcagaatgtc tttttttcac
    11521 cggagctgat tcgtcgcctg gcggatcggc acctgggggt agggtttgac cagctgctgg
    11581 tggttgagcc gccgtatgat cctgaactgg attttcacta tcgcattttc aatgctgatg
    11641 gcagtgaagt ggcgcagtgc ggtaacggtg cgcgctgctt tgcccgtttt gtgcgtctga
    11701 aaggactgac caataagcgt gatatccgcg tcagcaccgc caacgggcgg atggttctga
    11761 ccgtcaccga tgatgatctg gtccgcgtaa atatgggcga acccaacttc gaaccttccg
    11821 ccgtgccgtt tcgcgctaac aaagcggaaa agacctatat tatgcgcgcc gccgagcaga
    11881 caatcttatg cggcgtggtg tcgatgggaa atccgcattg cgtgattcag gtcgatgatg
    11941 tcgataccgc ggcggtagaa acgcttggtc ctgttctgga aagtcacgag cgttttccgg
    12001 agcgcgccaa tatcggtttt atgcaagtgg ttaagcgcga gcatattcgt ttacgcgttt
    12061 atgagcgtgg ggcaggagaa acccaggcct gcggcagcgg cgcgtgtgcg gcggtggcgg
    12121 tagggattca gcaaggtttg ctggacgaag aagtacgcgt ggaactcccc ggcggtcgtc
    12181 ttgatatcgc ctggaaaggt ccgggtcacc cgttatatat gactggcccg gcggtacatg
    12241 tctacgacgg atttattcat ctatgaagca actaggggaa gaactgcagg aaacactcac
    12301 ggagcttgat gaccgggcgg ttgtcgatta tctgattaaa aatcctgagt tttttatccg
    12361 caatgcgcgc gcagtagaag cgatacgtgt gccgcatccg gtacgcggca ccgtttcgtt
    12421 ggtcgagtgg cacatggccc gcgcacgtaa tcacattcat gttctggaag agaacatggc
    12481 gttgttgatg gagcaggcta tcgccaacga agggctgttt tatcgcctac tctacctgca
    12541 gcgcagtctc accgccgcca gcagtctcga cgatatgctg atgcgctttc accgctgggc
    12601 gcgcgatctc ggcctggctg gtgcgagtct gcgcctgttt ccagatcgct ggcgcttagg
    12661 tgcgccgtcg aaccacactc atctggcatt aagccgtcag tctttcgaac cgctgcgtat
    12721 tcagcgtttg gggcaggaac agcactatct tgggccgctt aacggaccgg agctgctggt
    12781 ggtgctaccg gaagcgaaag cggtgggatc ggtggcgatg tcgatgctgg gaagcgatgc
    12841 tgatttgggt gttgtgctgt ttaccagtcg cgatgccagt cactatcaac aagggcaggg
    12901 aacgcagtta cttcatgaaa ttgcgctgat gttgccggag cttctggagc gttggattga
    12961 acgcgtatga ccgatttaca caccgatgta gaacgctacc tacgttatct gagcgtggag
    13021 cgtcagctta gcccgataac cctacttaac taccagcgtc agcttgaggc gatcatcaat
    13081 tttgccagcg aaaacggcct gcaaagctgg caacaatgcg atgcagcgat ggtacgcaat
    13141 tttgctgtac gcagtcgccg taaagggctg ggagcagcaa gtctggcgtt acggctttct
    13201 gcgctacgta gcttttttga ctggctggtc agccagaacg aactcaaagc taacccggcg
    13261 aaaggagttt cggcaccgaa agcgccgcgt catctgccga aaaatatcga cgtcgacgat
    13321 atgaatcggc tgctggatat tgatatcaac gatcccctcg ctgtacgcga ccgtgcaatg
    13381 ctggaagtga tgtacggcgc gggtctgcgt ctttccgagc tggtggggct ggatatcaaa
    13441 cacctcgacc tggagtccgg cgaagtgtgg gtgatgggga aaggcagcaa agagcgccgc
    13501 ctgccgattg gtcgcaacgc tgtggcgtgg attgagcact ggcttgattt gcgcgacctg
    13561 tttggtagcg aagacgacgc gctttttctg tcgaaactgg gcaagcgtat ctccgcgcgt
    13621 aatgtgcaga aacgctttgc cgaatggggc ataaaacaag ggctgaataa tcacgttcac
    13681 ccacataaat tacgtcactc gttcgctacg catatgctgg agtcgagcgg cgatcttcgt
    13741 ggtgtgcagg agctactggg tcatgccaac ctctccacca cgcaaatcta tactcatctt
    13801 gattttcaac accttgcctc ggtgtacgat gcggcgcatc cacgcgccaa acgggggaaa
    13861 taatgcgttt ttaccggcct ttggggcgca tctcggcgct cacctttgac ctggatgata
    13921 ccctttacga taaccgtccg gtgattttgc gcaccgagcg agaggcgctt acctttgtgc
    13981 aaaattatca tccggcgctg cgcagcttcc agaatgaaga tctgcaacgc ctgcgccagg
    14041 cggtacggga agcggaaccc gagatttatc acgacgtgac gcgttggcgt tttcgttcga
    14101 ttgaacaagc gatgctcgac gccgggctga gtgccgaaga agccagtgca ggcgcacacg
    14161 cagcaatgat taactttgcc aaatggcgca gccgaatcga cgtcccgcag caaactcacg
    14221 acaccttaaa acagctggcg aagaaatggc cgctggtggc gatcaccaac ggtaacgccc
    14281 agccggaact gtttggtttg ggggattatt ttgagtttgt gctgcgcgct ggcccgcacg
    14341 ggcgctcaaa accgttcagc gatatgtact ttttggctgc ggaaaaactc aacgtgccga
    14401 tcggcgagat cttacatgtt ggggacgatc tcaccactga cgtgggtggg gcaattcgca
    14461 gcggaatgca ggcttgttgg atcagaccgg aaaatggcga tctgatgcaa acctgggaca
    14521 gccgtttact gccacatctg gaaatttccc ggttggcatc tctgacctcg ctgatataat
    14581 cagcaaatct gtatatatac ccagcttttt ggcggagggc gttgcgcttc tccgcccaac
    14641 ctatttttac gcggcggtgc caatggacgt ttcttacctg ctcgacagcc ttaatgacaa
    14701 acaacgcgaa gcggtggccg cgccacgcag caaccttctg gtgctggcgg gcgcgggcag
    14761 tggtaagacg cgcgtactgg tgcatcgtat cgcctggctg atgagcgtgg aaaactgctc
    14821 gccatactcg attatggctg tgacgtttac caacaaagcg gcggcggaga tgcgtcatcg
    14881 tatcggacaa ctgatgggca ccagccaggg cggcatgtgg gtaggcacct tccacgggct
    14941 ggcgcaccgc ctgctgcgtg cgcaccatat ggacgccaat ctgccgcagg atttccagat
    15001 cctcgatagc gaagaccagc tacgcctgct taagcgtctg atcaaagcca tgaacctcga
    15061 cgagaagcag tggccgccgc gccaggcaat gtggtacatc aacagccaga aagatgaagg
    15121 cctgcgtccg catcatattc aaagctacgg taatccggtg gagcagacct ggcagaaggt
    15181 gtatcaggcg tatcaggaag cgtgcgatcg cgcgggcctg gtggacttcg ccgagctgct
    15241 gctgcgcgct cacgaactgt ggcttaacaa gccgcatatc ctacaacact accgcgaacg
    15301 ttttaccaat atcctggtgg acgaattcca ggataccaac aacattcagt acgcgtggat
    15361 ccgcctgctg gcgggcgaca ccggcaaagt gatgatcgtc ggtgatgacg accagtcaat
    15421 ctacggctgg cgcggggcgc aggtggagaa tattcagcgt ttccttaatg atttccccgg
    15481 tgccgaaatt attcgtctgg agcaaaacta ccgctctacc agcaatattc tgagcgccgc
    15541 taacgccctg attgaaaaca ataacgggcg tctgggtaaa aaactgtgga ccgatggcgc
    15601 ggacggtgag cctatttccc tctattgcgc ttttaacgaa ctcgatgaag cgcgttttgt
    15661 ggttaaccgc atcaaaacct ggcaggacaa cggcggggcg cttgccgagt gcgccattct
    15721 ctaccgcagt aacgcccagt cgcgtgtgct ggaagaagca ctgttgcagg cgagtatgcc
    15781 gtaccgtatt tacggcggga tgcgcttctt cgaacgccag gaaatcaaag atgcgctctc
    15841 gtatctgcgc ctgattgcca accgtaacga cgacgcggcc tttgagcgtg tggtgaatac
    15901 gccaacgcgg ggtattggtg accggacgct ggacgtggta cgtcagacat cgcgcgatcg
    15961 ccagttaaca ctctggcagg catgtagtga actgttgcag gaaaaagccc tcgccggacg
    16021 tgctgccagc gccttacagc ggtttatgga actgatcgac gccttagcgc aggaaactgc
    16081 cgatatgccg ctgcatgtac agactgaccg ggtaattaaa gactccggcc tgcgtaccat
    16141 gtacgagcag gagaagggcg aaaaaggtca gacgcgtatc gaaaacttag aggaactggt
    16201 gacggcaacg cgccagttca gctacaacga agaagacgaa gatttaatgc cgctgcaggc
    16261 gttcctatcc catgcggcgc tggaagcagg cgaagggcag gcggatacct ggcaggatgc
    16321 ggtacaactg atgaccatgc actcggcgaa aggtctggag ttcccgcagg tgtttatcgt
    16381 cggtatggaa gagggcatgt tcccaagcca gatgtcgctg gatgaaggcg ggcgtctgga
    16441 agaagaacgc cgtctggcct acgttggcgt aacccgcgcg atgcagaaac tgacgctgac
    16501 ctacgcggaa actcgccgtc tgtatggcaa agaggtttac catcgcccgt cgcgctttat
    16561 tggcgagttg ccggaagagt gtgtggaaga ggtgcgcctg cgcgccacgg taagccgccc
    16621 ggtcagccat cagcggatgg gtacgccgat ggtcgagaac gacagcggct acaagctcgg
    16681 ccagcgtgta cgccacgcta agtttggtga aggcaccatc gtcaatatgg aaggcagcgg
    16741 tgaacatagc cgtttgcagg tggcattcca gggccaggga atcaaatggc tggtggcagc
    16801 ttacgcccgg ctggagtcgg tgtaacgttg ccggatgcgg tgctgcgcac cttatttggc
    16861 ctaaaaaatc attcagattc aataaattgc aacgtcatgt aggcaggata aggcgtttac
    16921 gccgcatccg gcatcagcag catatttagt atctgacaca aagctatcat tctaaccttt
    16981 ccgctcgacg gaaatcatgg tgacaaaagg atagcgttgc cacgggattg ccccgccttt
    17041 catatacata tgtgaaatcg tgccaccaag ataaagcagc tgctcaacgt tcagtttcgc
    17101 tttggcataa caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc
    17161 attcccatgt ttattaatcc caacaccgtt acgaatttta cgtgaggcga cgttgggatg
    17221 aatacgcgga ttaattacac cgttttccat caacattgct cctgactgca ccgcaaactg
    17281 aatatcttta ctggctttaa aagcatccag acgaacgatg ccgactttat ctcccgcgac
    17341 ataaaacacg ccgccaggac ggataaagaa attcccttca cctgaagcga gatttaacgc
    17401 taccttc
//
LOCUS       DADLTI010000081        17384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLTI010000081 DADLTI010000000
VERSION     DADLTI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.081"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            132..575
                     /locus_tag="JN755_002432"
     CDS             132..575
                     /locus_tag="JN755_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7381197.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JN755_002433"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JN755_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JN755_002434"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JN755_002434"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7381198.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JN755_002435"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JN755_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7381199.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JN755_002436"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JN755_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7381200.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JN755_002437"
     CDS             complement(4321..4470)
                     /locus_tag="JN755_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7381201.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="JN755_002438"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="JN755_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7381202.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="JN755_002439"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="JN755_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7381203.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="JN755_002440"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="JN755_002440"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7381204.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="JN755_002441"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="JN755_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7381205.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="JN755_002442"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="JN755_002442"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7381206.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="JN755_002443"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="JN755_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7381207.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="JN755_002444"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="JN755_002444"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7381208.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JN755_002445"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JN755_002445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7381209.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="JN755_002446"
     CDS             13936..14316
                     /locus_tag="JN755_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381210.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="JN755_002447"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="JN755_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7381211.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /locus_tag="JN755_002448"
     CDS             complement(15368..16087)
                     /locus_tag="JN755_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7381212.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JN755_002449"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JN755_002449"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7381213.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         4432 a         4540 c         4318 g         4094 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atag
//
LOCUS       DADLTI010000082        17104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLTI010000082 DADLTI010000000
VERSION     DADLTI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.082"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..171
                     /locus_tag="JN755_002450"
     CDS             <1..171
                     /locus_tag="JN755_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7381214.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(172..447)
                     /locus_tag="JN755_002451"
     CDS             complement(172..447)
                     /locus_tag="JN755_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7381215.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(468..704)
                     /locus_tag="JN755_002452"
     CDS             complement(468..704)
                     /locus_tag="JN755_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7381216.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="JN755_002453"
     CDS             complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="JN755_002453"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7381217.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2341..4839)
                     /gene="yebT"
                     /locus_tag="JN755_002454"
     CDS             complement(2341..4839)
                     /gene="yebT"
                     /locus_tag="JN755_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7381218.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4808..6092)
                     /gene="yebS"
                     /locus_tag="JN755_002455"
                     /pseudo
     CDS             complement(4808..6092)
                     /gene="yebS"
                     /locus_tag="JN755_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6222..6719
                     /locus_tag="JN755_002456"
     CDS             6222..6719
                     /locus_tag="JN755_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7381219.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6816..7514
                     /gene="proQ"
                     /locus_tag="JN755_002457"
     CDS             6816..7514
                     /gene="proQ"
                     /locus_tag="JN755_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7381220.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7534..9582
                     /gene="prc"
                     /locus_tag="JN755_002458"
     CDS             7534..9582
                     /gene="prc"
                     /locus_tag="JN755_002458"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7381221.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9774..10655
                     /gene="htpX"
                     /locus_tag="JN755_002459"
     CDS             9774..10655
                     /gene="htpX"
                     /locus_tag="JN755_002459"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7381222.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10701..12074)
                     /locus_tag="JN755_002460"
     CDS             complement(10701..12074)
                     /locus_tag="JN755_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381223.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12251..13042
                     /gene="kdgR"
                     /locus_tag="JN755_002461"
     CDS             12251..13042
                     /gene="kdgR"
                     /locus_tag="JN755_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7381224.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13185..13424)
                     /locus_tag="JN755_002462"
     CDS             complement(13185..13424)
                     /locus_tag="JN755_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381225.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13583..13726
                     /gene="mgrB"
                     /locus_tag="JN755_002463"
     CDS             13583..13726
                     /gene="mgrB"
                     /locus_tag="JN755_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7381226.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13801..14088
                     /locus_tag="JN755_002464"
     CDS             13801..14088
                     /locus_tag="JN755_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381227.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14758..14901
                     /locus_tag="JN755_002465"
     CDS             14758..14901
                     /locus_tag="JN755_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7381228.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14914..15123
                     /gene="cspE"
                     /locus_tag="JN755_002466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14914..15123
                     /gene="cspE"
                     /locus_tag="JN755_002466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7381229.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15289..16098
                     /gene="rlmA"
                     /locus_tag="JN755_002467"
     CDS             15289..16098
                     /gene="rlmA"
                     /locus_tag="JN755_002467"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7381230.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16095..16661)
                     /gene="mntP"
                     /locus_tag="JN755_002468"
     CDS             complement(16095..16661)
                     /gene="mntP"
                     /locus_tag="JN755_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7381231.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4523 a         4134 c         4314 g         4133 t
ORIGIN      
        1 tgtagccgct cgcgattagg tgaacgccca aaagggcagg gaattacagg tgctgatcat
       61 ttctggtttg gtcatacacc gttgcgacat cgcgtggata ttggcaacct gcattatatt
      121 gataccggtg ctgtctttgg gggcgaactg actcttgtgc aactgcaata attaaaaatc
      181 accatactcc tgtgctggtc gccagaaacc atctataaaa tcctcaatcg gaaaacaacc
      241 gccatggcgg atccgttgat cgctcataga ataaagacac tgctgttccg tgttgtagac
      301 atccacaaca atatcttcac aaccgccatc caggtagcaa acaaaaagta ccagcgcgaa
      361 catttcatcc ccgaagtgtg gtgccgtacc gttaagttta ggagagattt tacaacgggg
      421 gaataaccag gacaaataac ccgccatgcc gacgggttct ttttggatca ggcaagacgc
      481 ataatccagg catcagattg ttgatcgtaa cgggtacggt aaaggacaga gtgttcgcca
      541 tttagcaggg cagggatact ggtttcagca tcccaggcat ccagttgcat acataaatcg
      601 gggtcagatt tacaaggaat ggttaacgtt cgatcacccg gcgattcacc gtgtaattcg
      661 ccgtcgtcga tttcaaaagc gcctatgcgc acactggttt tcatcttcgt gctctccatt
      721 tagctgctga ttgtggtacg accagtccga tcgcacaatt tttcagccta gttgaggccg
      781 ccgaatgtgc cagtcaaaaa gtgcgctttt tttgtgcgct gtcatgcgtt actggtaaaa
      841 agtttcccgt cccgcaccag ttcacgcggg tagctgtttt tcagtcgcga accaatccgt
      901 ttggctaaac caatcggctg atgctggaaa gtaaccaaca cgtcatccgc cactggcgcg
      961 gcttgcgggt aaacatcgcg cccgcgatac cactcctccg cttcctgcgg tgtcagttca
     1021 aaagcgttta cattgtcggg ggaggcatgg gcaataaccg cttcatgctg ccagcgataa
     1081 cctttgttat gcgtttcggc aagtttaatc cctaagcgag aaaatcggac tttaccgatc
     1141 agggcttcaa tgcctaccgg gaacaaccac agttctttgt cgcgttgcca gagtcgcagg
     1201 tttccatccc agtttaagcc aacacttgca gccgcctgac gaatttgtcc ggcttcgcga
     1261 tctttcaccg ggctgaacgg gaaattgccc actttgtatt ttggggcggg taaggcgggg
     1321 atcgcctgag ttttacgcag acgagcaacg aagaagcctt cgcagtcgta aatttgtggg
     1381 aaaacatgca aaaaaccttc ttcggtcagc gccttgtttg caccagggaa gagatcgcca
     1441 agtggtaaaa actctacttc gtcggggtaa gtctctttca gccaccggca aacggcttcg
     1501 ttttcttccc ggtttaaggt acaggtcgag taaaccagcg taccgccagg acgtaatgca
     1561 tgaaaggcgc tgtcaatcag ttcccgttgg gtagctgcga tttcctgatt gctttcgggt
     1621 gaccagtttt ttagcgcatc gggatcttta cgcaccacgc cttcgccgga gcagggagcg
     1681 tccagcaaaa tggcatcaaa catttctggc actgacgcac caaacacgcg gccaccaaaa
     1741 tgtgtgagcg caacattact gatgccacag cggctgatat tggcatgtaa cacttttacc
     1801 cgactggcgg aaaactcatt ggcaaggatt gccccttcgt tattcatccg cgcggcaatt
     1861 tgcgtcgttt ttgagcctgg cgcagcagcg acatccatca cccgctgtgg tgcattaccg
     1921 tcagcaaaca aggcggcgac gggcaacatt gaactggctt cctgaatata aaacaggcca
     1981 cttaaatgct cggtggtact acccaatggc aatgcatctt cattgtcgcg ttcaatccag
     2041 aaaccttctt cacaccacgg aattggcgta agcgtccagc cataaggagc ggttaattgc
     2101 aggaaatcag caacggagat tttcagcgta ttaacgcgaa tgctgcggcg caacgggcgc
     2161 tgacaggcgg caagaaaatc atcaaatgag agcgtcgaag gcatagcttc gcgcatttgt
     2221 gtcagaaacg cgtccgggaa ataaacggcg tgttgggcca cgagcatgta ccactggcaa
     2281 aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga gcagtgggca
     2341 ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt cctgcaacag
     2401 gaagtgtttg ccttcctggg ctttcggtgc aagtggcgta cccggaggcg tggcgaaggc
     2461 gatgccgcca cggataaatt ggttaaaggt gccggttttc actacgccgc ccgtcagacc
     2521 aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca ccaggtgttg
     2581 atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat
     2641 tccggtaacc gtacctactt ccagaccacg gaacagcaca ggcgtaccga tgcctaacga
     2701 accggcttcc ggcgcttcaa caataatgct taaaccatct aggtaacgcg aatcagtaat
     2761 ggtggcctct tctaattcaa agtcgcgacg aggattgcca cggcctggtt cgacgttgat
     2821 atacggctgg aggatagtat ctagatgctc aacgccagct gccgagattt gcggtgtgac
     2881 cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat agagcaccgc
     2941 ctttgcctgt acttcattgc gtgcggtaat cagatccagc gtctggattt gcccgatatc
     3001 aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga aagcgtgaag
     3061 cgtaatctgc ctgccaaccg cacgggccgc tgtttcggaa gcatacagaa ttcgcttgtc
     3121 gcctttacgc tgactggcgc tggcaccact gaggttatcg aagctaatgg ccccctttaa
     3181 tgctctggag agcggggatg cctgtacggt caggccacta ccattcagct gaactttcgc
     3241 cccgccttct gcccagaaca cgctgttgct ggtcaaaagg ttgcgatatt ccggtttaat
     3301 gtgcagatcg atatcgaacg cgttagctcg cggacgcacg gtaataactt caccaacttc
     3361 aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa
     3421 actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt ccagattggc
     3481 atacagtgga tagctggctt tcatctcgcc tttatcgccc ggcagaatac gtatcccgcc
     3541 gttaatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc ccaccttcac
     3601 gtcgacacgg ctgttgacga caaatttgct atcgcctttt accagttctc gatgctgagg
     3661 ctcgatggcg acggtaaagg tgacgccttt gctggtgagt ttacgatcga taacctggcc
     3721 tacctgcacg ccgtgaagaa tgagcggctg acccgcatca ataccgtaac tttccggtgc
     3781 tgtcagggtc agtgtcagaa cgtccggctc ttgcagcagg gctttttcgc ccggcacaac
     3841 aacgaactct ttgcgtggct cgccatcgcc gggcaccaac tcaaaggttt ttccggtcag
     3901 cagggcgctg agattagcat cgctaaggga taatttcggg ttgcgtaatt ctatgcgggt
     3961 attttcacga agcagggtaa cgacgctggg atcaacggtc atttccccag tgactttacc
     4021 accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat acattaacgg
     4081 cgtcgagtcg gcggttaatc ctgctccact cggcagttcc agtttcacct tcgcgccact
     4141 gatactgacg ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcaccag
     4201 atcggtaaaa cgccgctcga tcaggacatc aatcaccacg ccttgcttgt tgggattgat
     4261 ggcatagtcg tagacttttc ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt
     4321 cagcgaaccg agatcggggg cttgcaggtg gatcatcaga tcgccattgt ccagccgata
     4381 tttcggttgg gtatcgagcg cgacaaagtg atcctgctct ttacctttac ccggcatcat
     4441 gccgatatag ttcccaccga cgagggcgtc cagcccggag acacctgcca acgatgcttt
     4501 tggcgtcatc agccagaact gagtctcttc gcgcagcgca tctttcatat cggacttgat
     4561 gctgaccttg acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc
     4621 gacttcaacg ccctgataac gaacaggcgt acggcccgga acaataccat ccgccgacat
     4681 aaagtcgatg gtgatggtat taccccggtc ctgataactg tcccaaatca gccaaccggc
     4741 aatcattagc gcgatgaaag gcagcagcca gaaaggtgag atacggcgtt tatttttaat
     4801 ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc
     4861 tgtccagcca ttccacagca agaatagtca aaattacggc agcgccaaaa taaaacgcag
     4921 ccggtcccat agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata
     4981 tgacaaacag gtcgagcatc gaccagcgtc caatccaggt caccatccgc agtaacagaa
     5041 tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct caacagtaaa gtgaacatga
     5101 cgatcacttt agtaaacggt accagaatac tggcgataaa cacgattccc gcaaccgcaa
     5161 tgttgctact tgccagcgac ataattccgg aaagaatcgt atcttcctgc cggccaccat
     5221 tcagataaat gatagaaata ggcaataggt tggctggtaa caacaaaacg attgacgcta
     5281 acagcgccgc ccagcatttt tgcaaactat gacgacgacg caggcgtagc gggatatggc
     5341 aacgcgggca gagaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac
     5401 gaagtttctc gtccctacgc gtagcggggc gctgcggata atatcgctcc cacagctcct
     5461 caacattaag atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca
     5521 agccgacacc cgcctggata tgggcataat cctgtacctt atatagaagc aacgccaatg
     5581 ccgaccaggt agatatccag cattacccac tctttaagtc gctcaagcat cagcagtacc
     5641 ggacgtaaat tcattcccag tcggttacca aaccataaat atgctatgga ggacaccaga
     5701 atgagggggg caccaataac gcagaaaaag accattgatc ccgttatcgc atcgccctgt
     5761 ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa tacgaatgcc taacagccag
     5821 atatgcaaca gcggttcgcc ccaggcaaac ggcataagca acagcatggt gaaggccatt
     5881 gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga
     5941 caataggcac tttgatgaga atttatctcg ggcaggctaa acagcatgtc acattgcggg
     6001 caacgttggt aatcaccacg cggcagttcc tcgccgattg ccctcactgt tatctttttt
     6061 gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc aaatgtaata agaattaaca
     6121 ttaatcttaa cccatgatgg gaatgatctt gagcattaac gcatttaatg cttattttaa
     6181 taggctggca gttccaggta agacaaccaa gtgattaaat aatgaacaaa acagaatttt
     6241 acgcggattt aaatcgcgac tttaacgcgc tgatggcggg agaaaccagt tttctggcaa
     6301 cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac tgacgttaac tgggcaggtt
     6361 tttatttgct tgaggacgat acactggtac tcggaccatt tcagggcaaa attgcctgtg
     6421 tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt tgcccgcaat caagtgcagc
     6481 gtatcgagga tgttcacgcg tttgacgggc atattgcctg tgatgcggcg agtaattctg
     6541 aaattgttct gccgctggtg gtgaaaaatc agattattgg tgttctcgac atcgatagta
     6601 ccgtcttcgc tcgctttaca gacgaggacg agcaaggctt acgtcagctt gtggcacagc
     6661 ttgaaaaagt gcttgcaacg acggattaca aaaaattctt tgcgagcgtc gcaggataat
     6721 caacggataa cgtagcattt actggtagcg tcattataat gacgcctgtt catgcctgcg
     6781 cttgttggct acgtccgttg taatcaggaa atttcatgga aaatcaacct aagttgaata
     6841 gcagtaaaga agtaatcgcg tttctcgccg aacgttttcc ccactgtttc agtgcggaag
     6901 gtgaagcgcg tccgctgaaa attggtattt ttcaggattt ggtcgatcgt gttgccgggg
     6961 aaatgaacct gagcaaaacg caattacgat ccgctttacg tctctacact tcgagctggc
     7021 gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg
     7081 gtgagctgga cgagcaacat gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc
     7141 gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg
     7201 gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg tccgaccacg ccacgccgca
     7261 aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag
     7321 taaaagcacc tcgcgaagaa cagcacaccc cggtttctga catttcagct ctgactgtcg
     7381 gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca
     7441 ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac
     7501 acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca tgttttttag gcttaccgcg
     7561 ttagctggcc tgcttgcaat agcaggccag accttcgctg tagaagatat cacgcgtgct
     7621 gatcaaattc cggtattaaa ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg
     7681 tcgcgcttca cccgttctca ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa
     7741 atctttgacc gctacctgaa tctgctcgat tacagccaca atgtgctgct ggcaagcgat
     7801 gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc
     7861 gacgttttct acgatctcta caatctggcg caaaagcgcc gttttgaacg ttaccagtac
     7921 gctttgtcgg tactggaaaa gccgatggat ttcaccggca acgacactta taaccttgac
     7981 cgcagcaaag cgccctggcc gaaaaacgag gctgagttga acgcgcagtg ggacagtaaa
     8041 gtcaaattcg acgagttaag cctgaagctg acaggaaaaa cggataaaga aattcgtgaa
     8101 accctgacac gccgctacaa atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat
     8161 gttttctcgc tggcaatgat ggcgtttgcg cgtgaaatcg atccgcatac caactatctt
     8221 tccccgcgta ataccgaaca gttcaacact gaaatgagtt tgtcactgga aggtattggc
     8281 gcagtgctgc aaatggatga tgactacacc gttatcaatt cgatggtggc aggtggtccg
     8341 gcagcgaaga gtaaagctat cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc
     8401 aagccgatgg ttgacgtgat tggctggcgt cttgatgatg tggttgcctt aattaaaggg
     8461 ccgaagggca gtaaagttcg tctggaaatt ttacctgctg gtaaagggac caagacccgt
     8521 actgtaacgt tgacccgtga acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg
     8581 aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc cgggcttcta tgtgggtttg
     8641 acagacgatg tcaaagttca actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc
     8701 atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg
     8761 tttattcctg cgggtcccat tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac
     8821 agcgataccg acgggcaggt gttctataaa ggcccactgg tggtactggt tgaccgtttc
     8881 agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt
     8941 gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat accgttcatt gaaccgtatt
     9001 tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt ctgtgcagta cacgatccag
     9061 aaattctatc gcgttaacgg cggcagtacg caacgtaaag gcgtaacacc agacatcatc
     9121 atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg
     9181 tgggatagca tcgatgccgc gacttatgtg aaatcaggag atttaactgc ctttgaaccg
     9241 gagctgctga aggaacataa tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg
     9301 aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca atatcgtttc tctgaactac
     9361 gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa
     9421 cgctttaaac gcgaaggtaa accggagttg aagaagctgg atgatctacc gaaagattac
     9481 caggagccgg atccttatct ggatgagacg gtgaatatcg cactcgatct ggcgaagctt
     9541 gaaaaagcca gacccgcgga acaacccgct cccgtcaagt aatatcaatc aggcacaaga
     9601 aattgtgcct gattttttaa cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa
     9661 agttgtgtct ttctggtgac ttacgcacta tccagacttg aaaatagtcg cgtaacccat
     9721 acgatgtggg tatcgcatat tgcgttttgt taaactgagg taaaaagaaa attatgatgc
     9781 gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt cgttttcggg ctggtactga
     9841 gcctgacagg gatacagtcg agcagcgttc aggggctgat gatcatggcc ttgctgttcg
     9901 gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg gatggcatta cgatctgttg
     9961 gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg ttggctggtc aatactgtag
    10021 caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt ggctatctac catgcgccgg
    10081 acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg
    10141 gtttgctgca gaacatgagc ccggatgaag ccgaggcggt aattgctcac gaaatcagcc
    10201 acatcgccaa tggtgatatg gtcaccatga cgctgattca gggcgtggtg aacaccttcg
    10261 ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg
    10321 atgaaggtga agagagcaac ggcaacccgc tgatctactt tgcggttgca acggttctgg
    10381 aactggtgtt tggtattctg gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat
    10441 tccatgctga tgccggttcg gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc
    10501 agcgcctgaa aaccagctat gaaccgcaag aagcaaccag catgatggct ttctgcatta
    10561 acggtaagtc gaaatcgctc agtgagttgt tcatgactca cccgccgctg gataaacgta
    10621 ttgaagctct gcgtacgggt gaatacctga agtaatatga agtaaatact aaaaagcgcg
    10681 tcctgatcga cgcgcttttt ttatgccctg gctcgtggct gagtgatacg taaaccactg
    10741 acacaggcag caatcactgc cagaatagcc gcagccatca gcgagacgtg cgtaccatta
    10801 tcaccaaact gatttagcat cagcgccacc agcgccgcgc cactactctg acccagtaga
    10861 cgagccgttc ctaacatgcc actggcccca ccgctacgtt cgcgcggagc ggaggtaata
    10921 atggtgtggt tattgggtga ctggaataag ccaaatccag cgccacataa gatcatcggc
    10981 cagataatat tgatatccgc aggtgacgcg ggcagcagaa ccagggaaaa aagccccgca
    11041 gccatgatga acaaccctaa agcacccagc aatcctgcat gtacgcgttc aatcaaatag
    11101 cctgccagcg gagccatcac catcgttgct aacggccacg gtgtcagaag taaacctgtt
    11161 tcgacttcac tacgcccgag cacggtttgc aggtaaaagg gcagggaaac cattgccagc
    11221 atttgtgcgc agaaagagca aacagatgtg caaatagaaa gtgaaaacag cgggatacgc
    11281 agtaaatcca ccggtagcag cggtacggga agagaaagct ggcggcgaat aaagaaaata
    11341 ccaacaacaa ccattaccac cagttccgca gcaattaacg tcagcgattg cccctgagcg
    11401 aaaccactca acgcagtgat aagcaggccg aaggttaacg cgttcatcac ggcgctgggc
    11461 aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga
    11521 agcagggcga taatacctaa cggtacgttg attaaaaata accatttcca ggatgcgatg
    11581 gagaggattg ctgcagcaat tgtcggcccg gcagcagaag agacggcaac aataaacgag
    11641 tttatgccca tccctctccc cagaaaacgt tgtggataga tcaggcggat aagtgcggta
    11701 ttaacgctca tcaacgctgc accgccgaaa ccttgtatga cacgcgcaag ggtgagcatt
    11761 tgcagcgaat cagaaagggc gcagaacagt gaagacaaca gaaaaacgac cagaccgcat
    11821 ttataaatac gtcgatagcc aaacatatcg cccagaaacg aaaacgagag cagggagatg
    11881 acaatggcga tttgataggc gttcactacc cagatggaac tggctggtgt ggcatgaagg
    11941 tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa
    12001 ataccaatca caatggttaa tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc
    12061 tgaacttttg gcataggaat tttatatctt tggtgaataa tcaacagggg tatgattcta
    12121 gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg
    12181 accaccctgg tgcgaaatta taataaaaaa cagttctgat ttttataaaa cactcgcaat
    12241 gaggtgataa atggctaacg cagatctgga taaacagcct gattctgtat cttccgtgct
    12301 aaaagttttt ggcattttgc aggcgctggg tgaagagcgc gaaataggga taaccgagct
    12361 gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc tttttacaga ccatgaaaac
    12421 cttaggttat gtggcgcagg aaggggagtc ggagaaatat tccctgaccc tgaaattgtt
    12481 tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt cgtagcgcag atatccagat
    12541 gcgtgagatc tcccgcctga ccaaagaaac tatccacctc ggcgcactgg acgaagacag
    12601 tattgtttac attcacaaaa ttgactctat gtacaatttg cgcatgtatt cacggattgg
    12661 gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg
    12721 cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa cgcagtaccg agcggaccat
    12781 caccagtaca gaagcgttat tacccgttct ggaccaggtg cgcgagcagg ggtatggcga
    12841 agataacgaa gagcaggaag aagggctgcg atgcattgcg gtaccggtat ttgatcgctt
    12901 tggcgtggtc attgccggtt tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg
    12961 tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg
    13021 ttatcacgac tatccgttct gatgagagta agaacctgtc ggaatatcaa acagacaggt
    13081 tctttattta gcatgagaaa aataaagttg aaggtgcgtt atattaaacg cgcttgctat
    13141 aagaatattt tactcatgag tgagaatctg gttatttatt acccttaacc attatcgacc
    13201 acgatattgc ttttacgtaa cagtgggcaa tctgttatcc ccaagaaacc acttttagtg
    13261 tgcaagtatt gtaccgtgct ggtgcctctg gccgtcagat aggtacattg caaacctaat
    13321 cctgcggcat tctctttgct tccaatcaaa acgccatatc cgctgagtaa taatcctatc
    13381 cataccagtg ctatcagcat aactgtgcga atgatgaatc gcattacaat ctcttctctt
    13441 tttatgttcg cttaatcgta gcggcaatat gcgctgaagc aagctactca ttccgaaaaa
    13501 gcacgaatat cgacatagtt aggcgctgtt taactaacgc atgctagttt aatgacataa
    13561 ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt
    13621 gttggcttgc ttgctgcttt gggcgcaggt attcaacatg atgtgcgatc aggatgtaca
    13681 atttttcagc ggaatttgtg ccattaacca gtttatcccg tggtgatatc attttttcag
    13741 cactagattt tcttcctctc cagcagtggt agaatagcgc ctctactcag aggtggtgaa
    13801 atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt ctttggttgt atcggtggtg
    13861 gttcttctta tcgggctcat cttgtggttt tttatcaatc gtgccagttc acggactaac
    13921 gaacagattg aactgcttga ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta
    13981 cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata aaaagaccat tgagagtcaa
    14041 aaatcggttg atgacgaaga tattattcgc ctggtcgccg aacgataatc cttcctcaat
    14101 cttgagtaac cccgtcgttg aagggttact caattcatcc tgattatacg cactgtcatg
    14161 gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa taattatcat attatttatt
    14221 tgcgtaacgg gatatcagaa aaagatattt tcctcattaa tttattagga tgtttacatc
    14281 agatttgtga ttaagcgtgg tattatttat tagcgaaacg tttctctctt gatttttttg
    14341 ctcactcatc aatttttctt attttaaatt tacaatccct ttggggattg acttctcttt
    14401 agggtaattg atagccgtta actgactgtt ttatgagaaa aagtgatata actttttatt
    14461 cattgcatag cagaaaatgt gatattgcac gcactatgta ataacttctc cctctggcct
    14521 ggaacaactg aacttattga actatgttag aaactacgcc agtttaagta tctgcctgaa
    14581 ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa
    14641 aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc
    14701 ataattaacg ttgtatgtga taaaagattt cgggttaaac gaggtacagt tctgtttatg
    14761 tgtggcattt tcagtaaaga agtcctaggt aaacacgttg acgttgaata ccgcttctct
    14821 gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg
    14881 cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag
    14941 tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag caaagatgtg
    15001 ttcgtacact tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac
    15061 gttgagttcg aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc
    15121 tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg
    15181 aatgcgaggc tttttactat gctttatctt cgctcctggc gttcggatat ttgccagcca
    15241 cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc
    15301 tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg tccccaacga
    15361 catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg
    15421 tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc
    15481 ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa
    15541 aaggccacgg cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc
    15601 gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct cgaaggtagc gataaaagcg
    15661 gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt
    15721 tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta
    15781 gcacgagtag tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg
    15841 atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa
    15901 ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt
    15961 gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc
    16021 tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc
    16081 tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc
    16141 agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca
    16201 accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg
    16261 ataatgttga cctgaaggaa agcaagacca acacccacag ccatggcatc caggctggtg
    16321 gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca
    16381 tcatctgagc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca
    16441 aacgcaatcc agtgattcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag
    16501 ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca
    16561 gaaaatttcg gtttatggag gggggcacct ttaccgattg atgcagcaaa tgcatccatc
    16621 gacataccaa acgcaagaag aacagtagca gtgatattca tgacaacgtc ctgaccgggg
    16681 tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat
    16741 gggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa
    16801 gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa
    16861 tctggaaatc ggctactccc caaatgaggg cgcaacctta acatattttg agaatataaa
    16921 acaactgttg gaatatatta tttcattgct ttattgataa cgattttcat ttatgtttat
    16981 atatcaatga aacgttaaaa taaataacaa ataagggaag agataatata gcctaagcta
    17041 tgtctgatta ttaaaaatac actaaacata gctttggcta aattcataag ttattgagtt
    17101 gata
//
LOCUS       DADLTI010000083        16826 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLTI010000083 DADLTI010000000
VERSION     DADLTI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.083"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(187..873)
                     /gene="kptA"
                     /locus_tag="JN755_002469"
     CDS             complement(187..873)
                     /gene="kptA"
                     /locus_tag="JN755_002469"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7381232.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JN755_002470"
     CDS             1020..1703
                     /locus_tag="JN755_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY7381233.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JN755_002471"
     CDS             1700..2161
                     /locus_tag="JN755_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381234.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JN755_002472"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JN755_002472"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7381235.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JN755_002473"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JN755_002473"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7381236.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JN755_002474"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JN755_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7381237.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JN755_002475"
     CDS             complement(4704..4787)
                     /locus_tag="JN755_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7381238.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JN755_002476"
     CDS             5380..6210
                     /locus_tag="JN755_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7381239.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JN755_002477"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JN755_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7381240.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JN755_002478"
     CDS             complement(7339..8799)
                     /locus_tag="JN755_002478"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7381241.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JN755_002479"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JN755_002479"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7381242.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JN755_002480"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JN755_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7381243.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JN755_002481"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JN755_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7381244.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JN755_002482"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JN755_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7381245.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13955)
                     /gene="fimF"
                     /locus_tag="JN755_002483"
                     /pseudo
     CDS             complement(13436..13955)
                     /gene="fimF"
                     /locus_tag="JN755_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13965..16601)
                     /gene="fimD"
                     /locus_tag="JN755_002484"
     CDS             complement(13965..16601)
                     /gene="fimD"
                     /locus_tag="JN755_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY7381246.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16668..>16826)
                     /locus_tag="JN755_002485"
     CDS             complement(16668..>16826)
                     /locus_tag="JN755_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HAY7381247.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         4086 a         4334 c         4096 g         4310 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagc ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccctg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agccatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt atagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcacagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gtcaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agttgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tacggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac aacgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcatgacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacagggtga cagcaaaata
    13741 cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc
    13801 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
    13861 tagccgcgga tagtaatcgt gctatccgca gccaaaacgc gacttaccgc cagccacaaa
    13921 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
    13981 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgacacag
    14041 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg
    14101 aggtaaaact gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
    14161 atcgccccaa acggcagcgg cttattattg tgagttagcg tcatgagcag ttttatccca
    14221 acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc
    14281 gcgttatcta aatcgacgtt atcagccaag gtattggtat ccagcgccac tctattttcc
    14341 cgatattcag tggcataagg cagcacggca taaccacgcc agtcggtacg cacccccgtc
    14401 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt
    14461 aacggctgcc ccagcgttac gccattggca tgagccaata ccccaccgct gactccgtaa
    14521 tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg
    14581 cgataattca gcgtggcgta gcctgtactt ccgctattac catcgcctcc cccggcatag
    14641 ccggtttgca cgctataact gaggttgttg tcttccagca acgtaccgta tacaccagcc
    14701 agattggtca tccgaccgtt gagatcgtgt gacatgctgt agctggcact ggcatgtcgc
    14761 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct
    14821 aacatctgat cccgtccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
    14881 ttgatatctt cgaacgcagt atttaatcca gcctggaatt gctcatcgac attactcgtt
    14941 ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga
    15001 gtaacggtga gctgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat
    15061 ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg
    15121 tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga
    15181 atattcgtgc ctgattcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca
    15241 tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc
    15301 agtgcctcca tgtttttccc gataccgaaa ttaaaagcac gataacgatc cgccagttgc
    15361 gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact ctggaaaaag
    15421 cggggttttt cctgctgcgc atttccacta cggtattctc ctgccataat ggaataacga
    15481 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg
    15541 ctgccgtcag cctctttgat cgttacctgc aagtcaccac tattacctgc ggcatagata
    15601 tcgttgatgg taaaaggccc cggtggcacc gtactattat aaatgccata tccattttgt
    15661 ttaatagtga cctgtgcagt accatgagca ataccgtgga tcaccggggc aaatcctctt
    15721 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
    15781 tcgaaaatat cgccctgggt ataaccatca cccaacgtca gccgggaacg taaaggaatt
    15841 atgtctcgct caatccaggt aatgatatgc tgccatttat ttttgctacc tgatgaactg
    15901 tcgctactgt tataactcca ggtggtattg tcgcgtaaac gccacgcacc aatatttaac
    15961 ccactctgta ggtttaaata tgcataatgg ctgttacccc caatccgatt ctgtacacta
    16021 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
    16081 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
    16141 tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa tggcacacag
    16201 gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc catactggcg
    16261 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
    16321 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
    16381 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
    16441 aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc ggcaaaagca
    16501 caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
    16561 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
    16621 cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta ttccattacg
    16681 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
    16741 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
    16801 agaacccggg ttccggcatt caactc
//
LOCUS       DADLTI010000084        16785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLTI010000084 DADLTI010000000
VERSION     DADLTI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.084"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..942
                     /locus_tag="JN755_002486"
     CDS             <1..942
                     /locus_tag="JN755_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY7381248.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..4064
                     /gene="mdtB"
                     /locus_tag="JN755_002487"
     CDS             942..4064
                     /gene="mdtB"
                     /locus_tag="JN755_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7381249.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            4065..7130
                     /gene="mdtC"
                     /locus_tag="JN755_002488"
     CDS             4065..7130
                     /gene="mdtC"
                     /locus_tag="JN755_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7381250.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVHDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7131..8546
                     /locus_tag="JN755_002489"
     CDS             7131..8546
                     /locus_tag="JN755_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381251.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8543..9946
                     /gene="baeS"
                     /locus_tag="JN755_002490"
     CDS             8543..9946
                     /gene="baeS"
                     /locus_tag="JN755_002490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7381252.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9943..10665
                     /gene="baeR"
                     /locus_tag="JN755_002491"
     CDS             9943..10665
                     /gene="baeR"
                     /locus_tag="JN755_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7381253.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10856..11188
                     /locus_tag="JN755_002492"
     CDS             10856..11188
                     /locus_tag="JN755_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7381254.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11335..12696
                     /locus_tag="JN755_002493"
     CDS             11335..12696
                     /locus_tag="JN755_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7381255.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13038..13355)
                     /locus_tag="JN755_002494"
     CDS             complement(13038..13355)
                     /locus_tag="JN755_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381256.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13761..14660
                     /gene="yegS"
                     /locus_tag="JN755_002495"
     CDS             13761..14660
                     /gene="yegS"
                     /locus_tag="JN755_002495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7381257.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14742..15521)
                     /locus_tag="JN755_002496"
     CDS             complement(14742..15521)
                     /locus_tag="JN755_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7381258.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15621..16661)
                     /gene="gatD"
                     /locus_tag="JN755_002497"
     CDS             complement(15621..16661)
                     /gene="gatD"
                     /locus_tag="JN755_002497"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7381259.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(16709..>16785)
                     /locus_tag="JN755_002498"
     CDS             complement(16709..>16785)
                     /locus_tag="JN755_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7381260.1"
                     /translation="LTGIFMTWRRARGFTKQEKAVLAE"
BASE COUNT         3883 a         4492 c         4400 g         4010 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtggacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg
     1141 tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga
     1201 aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa
     1261 cattaccgct caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact
     1321 tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc
     1381 cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg
     1441 tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt
     1501 ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg
     1561 gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag
     1621 gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg
     1681 ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg
     1741 atgtagcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag
     1801 aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg
     1861 acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga
     1921 cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat
     1981 tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg
     2041 cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg
     2101 tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc gccaccggat
     2161 tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg
     2221 aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc
     2281 tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc
     2341 gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt
     2401 cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac
     2461 agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc
     2521 gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc
     2581 tgctgcttag cgtgctgctg tgggtgttca ttccgaaagg tttcttcccg gtacaggaca
     2641 acggcattat tcagggcact ttgcaggcac cgcaatccag ctcctttgcc aatatggccc
     2701 agcgacaacg ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct
     2761 catttgttgg cgttgatggc actaacccgt cgctgaatag tgcacgttta caaatcaacc
     2821 tcaaaccgtt ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg
     2881 cggtagataa agtgccgggc gtcgatctct tcctgcaacc aacgcaggac ctgaccatcg
     2941 atactcaggt cagccgcacc cagtaccagt ttaccttgca ggccacgtca ctggatgcgc
     3001 tcagtacctg ggtgccacag ttgatggaaa aactccagca actgccacag ctttctgatg
     3061 tctccagcga ctggcaggac aaagggctgg tggcgtatgt caatgttgat cgcgacagcg
     3121 ccagccgtct ggggatcagc atggcggacg tcgataacgc cctgtacaac gcgtttggtc
     3181 agcgactgat ttccactatt tatactcagg ctaaccagta tcgcgtggtg ctggagcaca
     3241 ataccgaaaa taccccaggc ctcgcggcgc tggataccat tcgcctgacc agcagcgacg
     3301 gtggcgtggt gccgttaagc tcaattgcca aaattgagca gcgttttgcg ccgctttcca
     3361 tcaaccatct ggatcagttc ccggtaacaa ccatctcctt taacgtgccg gataactatt
     3421 cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa gacgctgaat ctgccggtgg
     3481 atatcaccac gcaattccaa ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg
     3541 tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga
     3601 gctttattca cccgatcacc attctctcga cgctacccac cgcaggggtc ggcgcgctgc
     3661 tggcattaat gattgccggt agcgaactgg atgtgattgc gattatcggc attattttgc
     3721 tgatcggtat tgtgaagaag aacgccatca tgatgatcga cttcgcgctg gctgctgagc
     3781 gcgagcaagg catgtcgccg cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc
     3841 cgatcctgat gaccaccctg gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg
     3901 gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat ggtcggcggt ctgattgtca
     3961 gccaggtgct gacgctgttc accacgccgg tgatttatct gctgttcgac cgcctggtat
     4021 tgtggaccaa aagtcgcttt gctcgccatg aagaggaggc gtaagtgaag ttttttgccc
     4081 tcttcattta ccgcccggtg gcgacgattt tactgtcggt cgccattacc ctgtgcggca
     4141 tactgggttt ccgtatgctg ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta
     4201 tggtcagcgc ctcgctgccc ggtgcgtcgc cggagacaat ggcgtcttcc gttgccacgc
     4261 cgctggagcg ctcacttggg cgcattgccg gggttagtga aatgacctcc agcagttcgc
     4321 tcggcagcac gcgcattatt ttgcagtttg attttgaccg ggatatcaac ggcgcagcgc
     4381 gtgatgtgca ggcggcgatc aacgctgcac aaagtttgct gcccagcggg atgcccagcc
     4441 gcccgaccta tcgcaaagcg aacccgtcgg atgcgccaat tatgatcctc acgctgacat
     4501 ccgatactta ttcgcagggt gaactgtacg atttcgcctc gacgcagctg gctccgacga
     4561 tttcgcaaat cgacggtgtc ggtgatgtcg atgtcggtgg cagctcgctg cccgccgtgc
     4621 gcgtcgggct gaacccgcag gcgctgttta atcagggcgt gtcgctggac gacgtacgca
     4681 ctgccatcag caatgccaac gtgcgtaaac cgcagggggc gctggaagat ggcactcacc
     4741 gctggcagat ccagaccaac gatgaactga aaaccgccgc tgaatatcag ccgctgatta
     4801 ttcactacaa caacggcggc gcggttcgtc tgggcgatgt ggcgacggtg actgactcag
     4861 tgcaggatgt gcgcaacgcc gggatgacca acgccaaacc ggctatttta ctgatgatcc
     4921 gcaaactgcc ggaagccaat attatccaga cggtagacag catccgggca aaattaccag
     4981 agttgcagga gaccattccg gcggcgattg atctgcaaat tgcccaggat cgctccccca
     5041 ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat tatctcggtg gcgctggtga
     5101 ttctggtggt ctttttattc ctgcgctcgg gtcgcgccac tattattccc gccgttgcgg
     5161 tgccggtttc gctgattggt acgtttgcgg cgatgtacct gtgcggattc agtctcaata
     5221 atctttcgct gatggcgctc accattgcta ctggtttcgt ggtggatgac gccattgtgg
     5281 tgctggaaaa cattgcccgt catctggaag cggggatgaa accactacag gccgcgctgc
     5341 aaggtactcg cgaagtcggc tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt
     5401 tcctgccgct gctgttgatg ggcggattgc cgggccgact gttacgcgag tttgccgtga
     5461 cgctttctgt cgccattggt atttcgttgc tggtttccct gacgctaacg ccaatgatgt
     5521 gcggctggat gctaaaagcc agcaagccgc gtgagcaaaa gcgactgcgt ggttttggcc
     5581 gcatgttggt agccctgcaa caaggctacg gcaagtcgct gaaatgggtg ctcaatcata
     5641 cccgtctggt gggcgtggtg ctgcttggca ccattgcgct gaatatctcg atcccgaaaa
     5701 ccttcttccc ggagcaggac accggcgtac tgatgggtgg gatacaggcg gatcagagta
     5761 tttcgtttca ggcgatgcgc ggcaaattgc aggatttcat gaaaattatc cgtgacgatc
     5821 cggcagtgga taatgtcacc ggctttaccg gcggttcacg agttaacagt gggatgatgt
     5881 ttatcaccct caagccacgc gacgaacgca gcgaaacggc gcagcaaatt atcgaccgtc
     5941 tgcgcgtgaa gctggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaagata
     6001 ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc
     6061 tggcggcact gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac
     6121 tggcggacgt gaactccgat cagcaggata acggcgcgga gatgaatctg gttcacgacc
     6181 gcgacaccat ggcacggctg ggaatcgacg tacaggccgc caacagtctg ttaaataacg
     6241 ccttcggtca gcggcaaatc tcgaccattt accagccgat gaaccagtat aaagtggtga
     6301 tggaagtgga tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg ttcgttatca
     6361 ataacgaagg caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaatgccc
     6421 cactatcggt gaatcatcag ggattatcgg cggcctcgac catctcgttc aacctgccga
     6481 ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg
     6541 tgccttcgac ggtgcgcggc agttttgccg gcacggcgca ggttttccag gagacgatga
     6601 actcgcaggt gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc
     6661 tttacgagag ttacgtacat ccgctgacga ttctctccac cctgccctcg gcgggcgttg
     6721 gagcgctgtt ggcgctggag ctgttcaatg ccccgttcag cctaatcgcc ctgataggga
     6781 tcatgctatt aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg
     6841 aagcccaacg gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc
     6901 gttttcgccc gattatgatg accaccctgg cggcgctgtt tggcgcgctg ccgctggtat
     6961 tgtcgggcgg tgacggctcg gagctgcggc aacccctgag gatcaccatt gtcggcggtc
     7021 tggtgatgag ccagctcctg acgctgtata ccacgccggt ggtgtatctc tttttcgacc
     7081 gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa atgacagatc
     7141 ttcccgacag cacccgctgg caattatgga ttgtggcttt cggcttcttt atgcagtcgc
     7201 tggacaccac catcgtaaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc
     7261 cgttgcatat gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg
     7321 ccagcggctg gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc
     7381 tgtttactct cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg
     7441 cacgcgcgtt acagggcgtt ggcggtgcga tgatggtgcc ggtcggcagg ttgacggtga
     7501 tgaaaatcgt accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc
     7561 aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct ggtagaatac gcgtcgtggc
     7621 actggatctt tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgatgt
     7681 taatgccgaa ctacaccatg cagacgcggc gctttgatct ttccggcttt ttattgctgg
     7741 cggttggcat ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc
     7801 cgctggcgat tgctggcctg gtcgcagttg gcgtggtggc actggtgctt tatctgctgc
     7861 acgccagaaa taacaaccgc gccctgttca gtctgaaact gttccgcact cgtacctttt
     7921 cgctgggcct ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga
     7981 caccggtttt cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga ctgatgatga
     8041 tcccgatggt gcttggcagc atgggaatga agcgaattgt ggtacaggtg gtgaatcgct
     8101 ttggttatcg tcgggtactg gtagcgacca cgctgggtct gtcgctggtc accctgttgt
     8161 ttatgactac cgctctgttg ggctggtact acgttttgcc gttcgtcctg tttttacaag
     8221 ggatggtcaa ctcgacccgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg
     8281 acaatctggc gagcagcgga aacagcctgc tgtcgatgat tatgcaattg tcgatgagta
     8341 tcggcgtcac catcgccggg ctgttgctgg ggctttttgg ttcgcagcat gtcagcgtcg
     8401 acagcggcac cacacaaacc gtctttatgt acacctggct tagcatggcg tttatcatcg
     8461 cccttccggc gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt
     8521 cgcggcgaaa aaggagcgcg caatgaagtt ctggcgaccc ggtattaccg gcaaactgtt
     8581 tctggcgatt ttcgccacct gcattgtctt gctgatcagt atgcactggg cggtgcgtat
     8641 cagttttgag cgtggcttta ttgattacat caaacacggc aatgaacagc gattacagct
     8701 gttaagtgat gcgcttggcg agcagtatgc gcagcatggt aactggcgct tcctgcgcaa
     8761 caacgatcgc tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa
     8821 acccggcccg ggtatgccgc cgcacggctg gcgtacccag ttctgggtgg tcgatcaaaa
     8881 caacaaagta ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat
     8941 tctggtcaac ggtgcggaag tgggcgcggt gattgcctcc cctgttgagc ggctgacccg
     9001 caatactgat atcaatttcg ataaacaaca gcggcaaacc agctggctga tcgtcgccct
     9061 ggcaacgtta ctcgcggcgc tcgccacctt tctgctggcg cgcggtttgc tggcaccggt
     9121 aaaacgactt atcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac
     9181 gcccaccagt gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc tcgccagcac
     9241 gctggagaaa aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg
     9301 tacgccatta gcggtgctgc gcggtgaact ggaagccatt caggatggcg tgcgcaaatt
     9361 cacgccggag acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga
     9421 cgatcttcat cagttgtcga tgtctgatga aggcgctctc gcctatcaaa aagcaccggt
     9481 agatttgatc ccactgctgg aagtggcggg cggcgcattt cgcgaacgat tcgccagccg
     9541 tggcctgaaa ctgcaatttt ccctgccaga cagtatcacc gtatttggcg atcgcgaccg
     9601 tttaatgcag ttattcaata acttactgga aaacagcctg cgctacactg acagcggcgg
     9661 cagcctgaaa atctctgccg agcagcacga caaaacggtg cgcctgacct ttgccgacag
     9721 tgcgccaggt gtcagtgacg atcagctaca aaaattgttt gaacgttttt atcgcaccga
     9781 aggttcccgc aaccgcgcca gcggcggttc cgggctgggg ctggcgattt gcctgaacat
     9841 tgttgaagcc cataatggcc gcattattgc cgcccattcg ccttttggcg gggtaagcat
     9901 tacagtagag ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc
     9961 gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc
    10021 attgattatc tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta
    10081 ctggcgtatg tgcgccagac accaccggat ctgatcctgt tggatctgat gctccctggc
    10141 accgatggcc tgacattatg ccgggaaatt cgtcgttttt ccgatgttcc gatcgtgatg
    10201 gtgacggcaa aaatcgaaga gatcgaccgc ctgctggggc tggagattgg cgccgatgac
    10261 tacatctgca agccgtacag cccacgggaa gtggtagcgc gtgtcaaaac cattttgcgc
    10321 cgttgtaaac cgcaacgcga acttcagcaa caggatgccg aaagcccgtt gattatcgac
    10381 gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg acctgacgcc tgcggaattt
    10441 cgtctgctta aaacgctctc tcacgaacca ggaaaagtgt tctcccgcga gcaattgctc
    10501 aatcatcttt atgacgacta ccgcgtagta accgaccgca ccattgacag ccacattaaa
    10561 aatctgcgcc gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac
    10621 ggcgttggtt accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacattac
    10681 tttgttagcc ggagatggcg ttcagacccg atccggcaac attattccac gcatggtcag
    10741 caaactgaca ttatctctcc gccctggcag gcatcagaaa atcgctcata ctttaatcag
    10801 taaacagcac ctttagatgc tgttttcgat aagcaatttc aatcaaggag tcattatggc
    10861 tggttggttt gaactcagta agagcagtga taatcagttc cggtttgtgc taaaagcggg
    10921 caatggtgag actatcctca ccagcgagct gtatacctca aaagcctctg cggaaaaggg
    10981 catcgcgtcg gtgcgtagca acagcccgca agaagagcgc tatgagaaaa aaacggcaag
    11041 caacggcaaa ttctatttca atctgaaagc cgctaatcat caaattatcg gctccagcca
    11101 gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc tccgttaaag ccaatggcac
    11161 aagccagacg gtgaaagaca atacgtaatt acgatgccgg gcgtgggttg ttggcgtccg
    11221 gcgctttacc agatcccccc tacagcgcta caatgcccgc ccttaaagtg ggggcctccc
    11281 ctaaccgctt catcaggtga agcggatctg acctgtcatc agaacgagag aattatgttt
    11341 aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat
    11401 ggcgcagatg ctgtttatgc gggccagccg cgttactccc tacgtgtgcg caacaacgaa
    11461 ttcaaccacg aaaatcttca gctcggcatc aatgaagccc acgcgctggg gaaaaagttt
    11521 tatgtcgtgg tcaacattgc accacacaac gccaagctga aaacctttat ccgtgacctg
    11581 aaaccggtgg tggaaatggg gccggatgcg ctgattatgt ccgatccagg gctgattatg
    11641 ctggtgcgtg agcacttccc agaaatgccg atccacctct cggtgcaggc taacgccgtg
    11701 aactgggcga cggtgaaatt ctggcagcaa atgggactga cccgcgtgat cctctctcgc
    11761 gagctgtcgc tggaagagat tgaagagatc cataatcagg tgccggatat ggagatcgag
    11821 atcttcgttc acggcgcact gtgcatggcc tactccggtc gctgcctgct ctctggctat
    11881 atcaacaagc gcgacccgaa ccagggcacc tgcaccaacg cctgccgctg ggagtacaac
    11941 gtccaggaag ggaaagaaga tgacgttggc aacatcgtac acaagtacga gccgattccg
    12001 gtgcaaaatg ttgagccgac gctgggtatc ggcgcgccaa ccgacaaagt gtttatgatc
    12061 gaagaagccc agcgtccggg cgagtatatg accgcgtttg aagatgagca cggcacttac
    12121 atcatgaact cgaaagatct gcgcgccatc gcccatgtag aacgcctgac caaaatgggc
    12181 gtgcattcgc tgaaaatcga aggtcgtacc aaatctttct actattgcgc acgcaccgca
    12241 caggtttacc gcaaagctat cgatgacgcc gctgcgggaa aaccgttcga taccagcctg
    12301 ctggaaactc tggaaggtct ggcgcatcgt ggctataccg aaggtttcct gcgtcgtcat
    12361 actcacgacg attatcagaa ctacgaatac ggttattcag tttctgaccg ccagcagttt
    12421 gttggtgagt ttaccggtga gcgcaagggg gacctcgcag cggtagcggt gaaaaataaa
    12481 ttctccgttg gcgacagtct tgagctgatg acgccgcaag gcaacattaa ctttaccctt
    12541 gagcacatgg aaaacgccaa aggcgaagct atgccggtcg caccaggcga tggttatact
    12601 gtgtggctcc cggtcccgca ggatcttgag ctaaattacg cgctgctaat gcgtaatttc
    12661 tccggggaaa ccacgcgtaa cccccacggt aagtgattaa tttcgattat ttttcccgga
    12721 tggaaaattc ttagaaaccg atcacataca gctgcattta ttaaggttat catccgtttc
    12781 gctgaaaaac ataatccata aaatgctagc tgtaccagga accacctcct tagcctgtgt
    12841 aatctccctt acacgggctt attttttgta cataagctgt aaagaaggat tgatccctga
    12901 taaccgtctt ccattgacca catcgataga atcccccttc atagcacaat gcctttcacg
    12961 taacggtatc gtgctcgccc aggttccggc tacgcacagc cagaacgcgc atgtttgacg
    13021 cttaccaaaa aatattctca ctctccacat ttgaatgtca gacgagcgac gccatgtaat
    13081 cctgcacctt ctgtcttcag gtcaactatc tgcatttttt tgccctgagt aacacagaaa
    13141 tgggctgcat cattttttac tatattttct gcaccagata ttctgcccct ggctaaagaa
    13201 gcttcggctt cggtgtagta ttggttatcg agtttacgct gaatattact tttatatgca
    13261 agaccaaatt taccgatact tgtttcatca ttatgcacag cacaaccaga tataagaaaa
    13321 atactaatta atgatatagc agctatcttt ttcatctcac cttcccccat taaataccaa
    13381 cgacactctc tagtgtttaa atataataat ggcatgatta ttataattga ataggattat
    13441 aataaatgtt ctgtacaaca tttcctacat aagtaggaat tacggacatt gaggcccttc
    13501 agggtaactc catgggggct ttaatatatt atattgaaga tgccactgtt tagttgaata
    13561 ttaggtatat gctctttttt gaaatttata ggtggcagcc gttagtattc gctgtcccca
    13621 ttgcaagctc ctggtggtaa ccactgaatc ctccatactt gaactgactt tttatcctcc
    13681 gactttcatc ctgttctgac tccacctttt gttttctgct ctacactatc tacagaccaa
    13741 tcataaaggc acatacgatc atggcagaat ttcccgccag cttactgatt cttaatggca
    13801 aaagtactga caatctaccc ttgcgcgaag caattatgct gttgcgtgag gaaggaatga
    13861 cgatccatgt gcgggtcacc tgggagaaag gtgatgccgc acgatttgta gaggaggccc
    13921 ggaagttggg cgtcgcaacg gtgattgccg gtggtggcga tggcaccatt aatgaagttt
    13981 ctacggcgtt gattcagtgt gagggggatg acatacccgc gctgggaatt ttgccattag
    14041 gaaccgccaa tgattttgcc accagtgtag ggattcctga ggcactggat aaggcgctga
    14101 aactggcaat tgccggtaat gccattgcga tagatatggc gcaggtcaac aaacaaacct
    14161 gttttattaa tatggcgaca ggcggatttg ggacgcgtat taccacagaa acgccggaaa
    14221 aattaaaagc cgcgctgggt ggcgtctctt acatcattca tggcttaatg cgcatggata
    14281 ctctgcaacc ggaccgttgt gaaatccgcg gtgaaaactt tcactggcaa ggtgacgccc
    14341 tggtcattgg tattggtaac gggcgtcagg ccggtggcgg tcaacaattg tgcccgaacg
    14401 cgttaattaa cgatggcttg ctgcaactgc gcatttttac cggcgatgaa attcttccgg
    14461 ctctcgtatc aaccttaaaa tctgacgaag ataacccgaa tattatcgaa ggcgcttcgt
    14521 cgtggtttga tatacaagcc ccacacgaaa tcacttttaa tcttgatggc gaaccgttga
    14581 gtgggcaaaa cttccatatt gaaatacttc cggcggcgtt gcgttgtcga ttaccaccag
    14641 attgtccatt attgcgttaa tcaaactatt ctctcttgaa ccagggagaa tccgtaatgc
    14701 cggatgcggt gtaaacacct tatccggcat acagaacaat actacaccgt aattaatttt
    14761 acccgtgtct tctgcaatgc caatttatcc acctctgaca cagcatcatc ggtaacaata
    14821 atgtcaattt tttcgattgg cagtacctga ttaaaaccgc gacgattaaa tttcgatgaa
    14881 tcgagaacgg caatcacttt atttgcggcg gtgaccatcg ccccgctaat cgaataacct
    14941 tcattaaagg tcgtaatacc attaaccgca tcaataccat cagcaccgac aaacattaaa
    15001 tcggcattaa tatcctgcaa tgaacgctcg gcaatggaac catgcatcga gcgcgtttta
    15061 tggcggaccg tgccaccaca gacgaccaga gtaatgtctt tattttcgga aagggcaaac
    15121 gctgccggga gactgttggt aatcaccgtg atatttttag cggtcattaa tccttcggcg
    15181 ataagcattg tggtacttcc gctgtcgaga ataacggtca tcccttcatg gatcattttg
    15241 actgccgcct gcgctattct gttttttggc gcgctggcga gctgaaatcg ctctttaaac
    15301 ccgacttcct gggtctcggt ttcactatta ccggacatta ttttcgccgc accgccatga
    15361 aagcgcgtaa ccacgccttt ttgttcgaga aagcgcaaat cggcacggat tgtcgcttcc
    15421 gaggcagcaa atactcccgc cagatcctga acaagcacgg ttccctgttc attcactaat
    15481 tggatgatct tattccttcg ctcgaatgaa ttcatcgtca tttacccata ttcaattgtg
    15541 gctagtgtaa acgaaggggg accattggtg aatgatctgt tttgcacaat accgggccag
    15601 catcacgctg gcccgcggtt tcatggaatg agcaacactt tgcccggcat ggcattacga
    15661 gcgatgtcac gcaccgcctg ggtgaagctt tcaaagcttc cacggtgagc gattaatgga
    15721 tccaggctta acttacgttc tgtcagcaac cggctcgccg tttcccactc ctgccccggc
    15781 caagggctgg aatagttcat ccaactgccg ataaccgtca gctctttacg caatattttg
    15841 ccaaacgttg ccgatgttaa atgcagatcc tgatgcaacg tgcccaccag cgccagttgg
    15901 gcatgaggac cggcaatctc taccgccagt tcgacagttt gcggtacgcc agccgtctcg
    15961 aggataagct gattaaagcg cagttcgcgt aaaacgctct gcatttgcgg cgcgctcatt
    16021 tcgctactgt taaatgtttg catcgcaccg aaagattttg ccagtgccag tttttctgag
    16081 ctaatgtcga tcgccgtcac actctttgct cccagcgcga cagcgcactg aatagccagc
    16141 aggccaatgg ttccggcacc aataataata acgtttttat tctcacaacc ttgcgctaaa
    16201 tgaaaagcat gcaggccaac ggtaatcggc tcaataaaag ccccatcctc aataggcata
    16261 tccgtgggta gagcaaagac attttttcgc ttaacgacaa tatattcagc aaatccacca
    16321 tcacgccgcg agccaataaa atcatatttt gcgcactggg aataaaaccc tttcaaacac
    16381 tctggacaag taaaacaggg taataacggc acacaggcaa ccgcatcgcc agggtgtaaa
    16441 tcatcaacac cggatcccac cgcatcaata tagccgctaa attcatggcc taacgttatt
    16501 ggataataat gtgcaccatt tttaaatatc ctgggtaaat cggaaccaca taagcccgag
    16561 ctggcaattt ttacccgcac ctcatcctga tgtttaattt caggaatgac gctttttgca
    16621 acgcgcacga taccatcagt atcattcact actgatttca taaaaactcc tgattgttaa
    16681 gggggataac ccccccctca ggtaaaaatt attctgcgag aacggctttc tcttgtttag
    16741 taaaaccacg cgctctacgc caggtcatga aaataccggt cagat
//
LOCUS       DADLTI010000085        16755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLTI010000085 DADLTI010000000
VERSION     DADLTI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.085"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN755_002499"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN755_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7381261.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNVQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN755_002500"
     CDS             2219..2767
                     /locus_tag="JN755_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7381262.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN755_002501"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN755_002501"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7381263.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN755_002502"
     CDS             complement(3664..4854)
                     /locus_tag="JN755_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7381264.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN755_002503"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN755_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7381265.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN755_002504"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN755_002504"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7381266.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN755_002505"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN755_002505"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7381267.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="JN755_002506"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="JN755_002506"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7381268.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JN755_002507"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JN755_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7381269.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="JN755_002508"
     CDS             complement(13353..14000)
                     /locus_tag="JN755_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7381270.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JN755_002509"
     CDS             complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JN755_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7381271.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15154..16113)
                     /locus_tag="JN755_002510"
     CDS             complement(15154..16113)
                     /locus_tag="JN755_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7381272.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JN755_002511"
     CDS             complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JN755_002511"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7381273.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4184 a         4320 c         4291 g         3960 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgtgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttttggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc cttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgtgg
    14881 cggcctggcg ggcggcaaca gtaggtgagg ttacgctggg acgcagggcg acaagaaact
    14941 tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag ccacgcatct tcacacgatc
    15001 gccccgttgg gatcagcaca ccggtatagc ccagacgatc cgccgcttgc gcaatttgtt
    15061 gcagataacc gtgatcaacc gggcgtgaac cttcttccgt tcccagatag tgcccgtcac
    15121 cgtgggtcgg taaaaaccag aacatattca gactcataat tgttttcctt ccagttgagt
    15181 gggctgccag atgcgctggc gaatatcgac ttttttcggc accagacggt tttcataaaa
    15241 cagatctgcc gtttgctgct gtaaggcggc aacctcggcg ttaaccggtt tgatggtggt
    15301 aggagggcga tgatctaagt aagaggcaat caccggtgcc ggtaagccaa tcgtttttgc
    15361 cagtaaagcg atgctttgtt cgcgctggct gcgggttaac gcatcggcct cactaaaggt
    15421 tgccagtacg ccctgaataa aagcgccgtt tttttctgca tagggacgag ctgccagata
    15481 aaacgatcca gtttgattga gatcggtgcc gtctttcagc acccgcacgc cgccctgtaa
    15541 taacgcagcg gagtagtagg gatcccagat agcccaggcg tcaacgttac cttgctggaa
    15601 cgcggcgcgg gcaccggcag gcgtcaggta agtgggctgg atatcggtga atttaagtcc
    15661 ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg gaacctttct gaaaggcaac
    15721 tttgtgacct ttaagatcgg ctacggtttt gatcgggctg ttttctgcca ccagaatcac
    15781 ttcggctttg ggcttcggtg gctcgacgcc cacatatacc aaatcagccc cggcagcctg
    15841 ggcaaagatt ggcggaatat ccccggtact gccgagatca atactgccaa cgtttaacgc
    15901 ttccaacatt tgcggacccg cggggaactc cacccaggag atttttgatt gcggatagcg
    15961 tttttccagt aactggtggc tttttgccag caccatacca atactgcctt tctgatagcc
    16021 tatacgtaac gcttcaggcg aggattctgc agcaaccgca aaagtagaga cgctaagcaa
    16081 tcccgccagc gccagtttaa tgatgttacg catgggcatt acctcgcaga gacagaaggt
    16141 caggaacctg aacatcgcgg cggtgcaatg cctgccagaa agtttctagc gcggtatcaa
    16201 gatggggttg cagatttggc gtgaactggg gtctgtgatg gtaatcaatt aattgtgagt
    16261 catcggcaaa cacgccgtgc aggatctcct gagctttcag tgcgcttaaa actggtttaa
    16321 gggcataatc gaccgccagc agatgggcca cggtaccgcc cgtcgccagc ggtagcacca
    16381 ctttgccttg caaagcgcgc tctggcagca ggtcgagcag ggttttcagc gcgccggaat
    16441 aggcggcttt atacacaggc gtggcgacaa tcagcccatc agcctgttgc agctgttcgg
    16501 tgaaggtctt gagtgccgga ctatcgaaac gagcataaag cagatcttcc ggggcgaagt
    16561 tttgcagatt ccagtgataa acctctacat ctaggccatt tagtttttcc cgcgcatatt
    16621 ccagcaagga gctggagcga gaaggaaagc gaggactacc cgccagggtg atgacacgca
    16681 tactctctcc ttataaccaa ttgttctttt ttttgttaac attgataaca attcggtcag
    16741 tctgtcggag agaca
//
LOCUS       DADLTI010000086        16683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLTI010000086 DADLTI010000000
VERSION     DADLTI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.086"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..2081
                     /gene="pbpC"
                     /locus_tag="JN755_002512"
                     /pseudo
     CDS             1..2081
                     /gene="pbpC"
                     /locus_tag="JN755_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2231..2662
                     /gene="ndk"
                     /locus_tag="JN755_002513"
     CDS             2231..2662
                     /gene="ndk"
                     /locus_tag="JN755_002513"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7381274.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JN755_002514"
     CDS             2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JN755_002514"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7381275.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4251..5264
                     /gene="rodZ"
                     /locus_tag="JN755_002515"
     CDS             4251..5264
                     /gene="rodZ"
                     /locus_tag="JN755_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7381276.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5291..6409
                     /gene="ispG"
                     /locus_tag="JN755_002516"
                     /gene_synonym="gcpE"
     CDS             5291..6409
                     /gene="ispG"
                     /locus_tag="JN755_002516"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7381277.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6520..7794
                     /gene="hisS"
                     /locus_tag="JN755_002517"
     CDS             6520..7794
                     /gene="hisS"
                     /locus_tag="JN755_002517"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7381278.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7812..8432
                     /locus_tag="JN755_002518"
     CDS             7812..8432
                     /locus_tag="JN755_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7381279.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8443..9621
                     /gene="bamB"
                     /locus_tag="JN755_002519"
     CDS             8443..9621
                     /gene="bamB"
                     /locus_tag="JN755_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7381280.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9739..11211
                     /gene="der"
                     /locus_tag="JN755_002520"
     CDS             9739..11211
                     /gene="der"
                     /locus_tag="JN755_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7381281.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11281..11496
                     /locus_tag="JN755_002521"
     CDS             11281..11496
                     /locus_tag="JN755_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381282.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JN755_002522"
     CDS             complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JN755_002522"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7381283.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13025..14491
                     /gene="guaB"
                     /locus_tag="JN755_002523"
     CDS             13025..14491
                     /gene="guaB"
                     /locus_tag="JN755_002523"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7381284.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14560..16137
                     /gene="guaA"
                     /locus_tag="JN755_002524"
     CDS             14560..16137
                     /gene="guaA"
                     /locus_tag="JN755_002524"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7381285.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16232..>16683)
                     /locus_tag="JN755_002525"
     CDS             complement(16232..>16683)
                     /locus_tag="JN755_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381286.1"
                     /translation="EAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAA
                     TAAANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFID
                     QELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3844 a         4171 c         4671 g         3997 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc
      661 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      721 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      781 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      841 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
      901 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
      961 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1021 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1081 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1141 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1201 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1261 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1321 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1381 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1441 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1501 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1561 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1621 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1681 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1741 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1801 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1861 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     1921 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     1981 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2041 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2101 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2161 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2221 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2281 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2341 caaaatgctg cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2401 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2461 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2521 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2581 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2641 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2701 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2761 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2821 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     2881 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     2941 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3001 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3061 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3121 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3181 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3241 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3301 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3361 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3421 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3481 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3541 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3601 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3661 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3721 tgtaagatca acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg
     3781 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3841 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     3901 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     3961 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4021 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4081 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4141 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4201 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4261 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4321 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4381 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4441 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4501 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4561 aacgccgcaa aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg
     4621 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4681 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4741 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4801 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4861 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     4921 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     4981 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5041 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5101 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5161 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5221 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5281 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5341 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5401 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5461 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5521 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5581 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5641 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5701 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5761 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5821 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     5881 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     5941 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6001 cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6061 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6121 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6181 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6241 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6301 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6361 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg
     6421 gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag
     6481 cgttgagggt tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc
     6541 attcgcggca tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc
     6601 acactgaaaa acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag
     6661 cagaccccgc tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg
     6721 tacacctttg aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc
     6781 tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat
     6841 atcgggccga tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag
     6901 ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc
     6961 actgcccgct ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct
     7021 atcggttcgc tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag
     7081 cataaagaaa agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg
     7141 ctggattcaa aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat
     7201 tatctggacg aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg
     7261 gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc
     7321 gttttcgagt gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt
     7381 tatgacggtc ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg
     7441 ggcctcgaac gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct
     7501 gttgtcgata tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta
     7561 gctgagcgtc tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc
     7621 aactttaaga aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg
     7681 ggtgagtctg aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa
     7741 acggcagttg cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg
     7801 agaaggacag cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct
     7861 tttttgctga aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga
     7921 ttggctggcg ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct
     7981 atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa
     8041 aatttgctgc tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc
     8101 aatttgttga caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag
     8161 acacgagcga tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac
     8221 agctcaagca ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg
     8281 ctgccattgt tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg
     8341 cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga
     8401 tgcagatgaa aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta
     8461 ctgctgccag gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa
     8521 gaagatgtgg taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg
     8581 gcgtggagca cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca
     8641 ctggcggaca acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg
     8701 gatgatggca aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa
     8761 gagcctgcat tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc
     8821 gaaaaggcgc aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca
     8881 gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc
     8941 agtaacggtc agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac
     9001 ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg
     9061 gccgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg
     9121 atttggcagc agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat
     9181 gttgacacga ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac
     9241 ctgacggcgc ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg
     9301 aatgatttca tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg
     9361 gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg
     9421 ctgacttctc cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg
     9481 cactggatta acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt
     9541 ttccagactg aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc
     9601 gtgtactcta ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag
     9661 gggccgtttt cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat
     9721 aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga
     9781 aaatccacgt tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg
     9841 ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt
     9901 attgataccg gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag
     9961 tcgctgctgg cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc
    10021 ctgatgccgg cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc
    10081 ctggtggcaa acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg
    10141 cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg
    10201 ctggagcatg tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa
    10261 gacgctgaat actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac
    10321 gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt
    10381 aagtctacac tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct
    10441 ggcacgacgc gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc
    10501 attgacaccg ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc
    10561 gtaatcaaaa cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg
    10621 cgcgaaggta tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc
    10681 tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg
    10741 aaagaaacgc tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct
    10801 gccttgcacg gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc
    10861 tccacccgtc gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat
    10921 caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt
    10981 tataacccgc cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac
    11041 aagcgctact tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt
    11101 attcagttca aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc
    11161 cagatgcgta aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta
    11221 agtattttat ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat
    11281 atggaactgc attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt
    11341 tgcccatcct gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg
    11401 ttgcaggtgt taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg
    11461 atatcgaaaa aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt
    11521 accggctgga tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc
    11581 atttcacccg ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta
    11641 acgctgtatc cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca
    11701 ttaccgaaac gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat
    11761 tccagttgct gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc
    11821 agccgctgtg ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca
    11881 aagctcatcc gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc
    11941 ggatgctgct gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga
    12001 taataatcca tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct
    12061 tgctgattac ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca
    12121 acaaaatcgg caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg
    12181 ctggcaaaaa tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa
    12241 ccgccgccgc gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg
    12301 gcgcgaacaa tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc
    12361 ggtagagaag gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt
    12421 tttgaggtga tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga
    12481 tcgaacaaac cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc
    12541 ccttcaccgg ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag
    12601 agcgtaatat tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg
    12661 cggttgctgt tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag
    12721 taccagtgac cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt
    12781 cccatctcat gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt
    12841 gcaggggatt gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca
    12901 gtcgatagta acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca
    12961 atcggttacg ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt
    13021 gcccatgcta cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc
    13081 tcattctacc gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg
    13141 tctgaatatc cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat
    13201 tgctctggct caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca
    13261 ggcagaagaa gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac
    13321 tgttctgcca accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc
    13381 gggctatccg gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt
    13441 gcgttttgtt accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct
    13501 ggtcaccgtg cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg
    13561 cgttgaaaaa gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa
    13621 agacttccag aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg
    13681 tgttggtgct gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc
    13741 cgctggcgtt gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca
    13801 gcgtatccgt gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc
    13861 aacagctgca ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat
    13921 cggccctggt tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac
    13981 tgccgttgct gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg
    14041 tggtattcgc ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat
    14101 ggtaggttcc atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg
    14161 ccgttcttat aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc
    14221 tgaccgttat ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg
    14281 tcgcgtagct tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg
    14341 ctcctgtatg ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt
    14401 tgtacgtatc agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa
    14461 agagtccccg aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga
    14521 tttatattat ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag
    14581 catcgcatcc tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg
    14641 cgtgagctgg gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt
    14701 gacttcaatc caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac
    14761 agtccgcgtg cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat
    14821 ggcatgcaga ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa
    14881 tttggctacg cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat
    14941 gcgctgaccg cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt
    15001 accgctatcc cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt
    15061 atggctaacg aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc
    15121 cgccagggta tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg
    15181 tggacgccag cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac
    15241 gataaagtca tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg
    15301 caccgtgcta tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc
    15361 aacgaagcag agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac
    15421 gtaccggcag aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa
    15481 cgtaaaatca tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa
    15541 gacgtgaagt ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct
    15601 gcaaccggta aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag
    15661 atgaagatgg gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt
    15721 ggtctggagc tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc
    15781 cttggcgttc gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct
    15841 gacgccatct tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg
    15901 ttcaccgtct tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac
    15961 tgggttgtct ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat
    16021 ctgccgtacg atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt
    16081 tcccgcgtgg tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt
    16141 tgatccctgc actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca
    16201 agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct
    16261 cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg
    16321 cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga
    16381 taaattcgtt ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct
    16441 gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg
    16501 cttcggcgtc cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt
    16561 tgttcgcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc
    16621 gctccgcgac ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgttccgctt
    16681 ctg
//
LOCUS       DADLTI010000087        16380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLTI010000087 DADLTI010000000
VERSION     DADLTI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.087"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            7..375
                     /locus_tag="JN755_002526"
     CDS             7..375
                     /locus_tag="JN755_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381287.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JN755_002527"
     CDS             complement(411..1367)
                     /locus_tag="JN755_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7381288.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JN755_002528"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JN755_002528"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7381289.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JN755_002529"
     CDS             complement(2605..3366)
                     /locus_tag="JN755_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7381290.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JN755_002530"
     CDS             3499..3909
                     /locus_tag="JN755_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381291.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JN755_002531"
     CDS             complement(3871..4977)
                     /locus_tag="JN755_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7381292.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JN755_002532"
     CDS             complement(4988..6121)
                     /locus_tag="JN755_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7381293.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JN755_002533"
     CDS             complement(6114..7850)
                     /locus_tag="JN755_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7381294.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JN755_002534"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JN755_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7381295.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JN755_002535"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JN755_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7381296.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JN755_002536"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JN755_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JN755_002537"
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JN755_002537"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY7381297.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JN755_002538"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JN755_002538"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7381298.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="JN755_002539"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="JN755_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7381299.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15018..16103
                     /locus_tag="JN755_002540"
     CDS             15018..16103
                     /locus_tag="JN755_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7381300.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         3823 a         4343 c         4439 g         3775 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cttggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtggac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcataa ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
//
LOCUS       DADLTI010000088        16326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLTI010000088 DADLTI010000000
VERSION     DADLTI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.088"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="JN755_002541"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="JN755_002541"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7381301.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JN755_002542"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JN755_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7381302.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="JN755_002543"
     CDS             2341..2556
                     /locus_tag="JN755_002543"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381303.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="JN755_002544"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="JN755_002544"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7381304.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /locus_tag="JN755_002545"
     CDS             4451..5845
                     /locus_tag="JN755_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7381305.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="JN755_002546"
     CDS             5922..6419
                     /locus_tag="JN755_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7381306.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="JN755_002547"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="JN755_002547"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7381307.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="JN755_002548"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="JN755_002548"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7381308.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="JN755_002549"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="JN755_002549"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7381309.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9032..9667
                     /locus_tag="JN755_002550"
     CDS             9032..9667
                     /locus_tag="JN755_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7381310.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="JN755_002551"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="JN755_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7381311.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="JN755_002552"
     CDS             complement(10259..11239)
                     /locus_tag="JN755_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7381312.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="JN755_002553"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="JN755_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7381313.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="JN755_002554"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="JN755_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7381314.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="JN755_002555"
     CDS             12542..12832
                     /locus_tag="JN755_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7381315.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="JN755_002556"
     CDS             12840..13433
                     /locus_tag="JN755_002556"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7381316.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="JN755_002557"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="JN755_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7381317.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="JN755_002558"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="JN755_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16326
                     /locus_tag="JN755_002559"
     CDS             15884..>16326
                     /locus_tag="JN755_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY7381318.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4041 a         4208 c         4109 g         3968 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc tacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa aaaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggcctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ttgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtctcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatagccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacaatca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcacgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 tcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 tacccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgc
//
LOCUS       DADLTI010000089        16149 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLTI010000089 DADLTI010000000
VERSION     DADLTI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.089"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="JN755_002560"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="JN755_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7381319.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JN755_002561"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JN755_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7381320.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="JN755_002562"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="JN755_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="JN755_002563"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="JN755_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY7381321.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="JN755_002564"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="JN755_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7381322.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="JN755_002565"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="JN755_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY7381323.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="JN755_002566"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="JN755_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY7381324.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="JN755_002567"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="JN755_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY7381325.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="JN755_002568"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="JN755_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY7381326.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="JN755_002569"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="JN755_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY7381327.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="JN755_002570"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="JN755_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY7381328.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="JN755_002571"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="JN755_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY7381329.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="JN755_002572"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="JN755_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY7381330.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="JN755_002573"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="JN755_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY7381331.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="JN755_002574"
     CDS             11058..11762
                     /locus_tag="JN755_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY7381332.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="JN755_002575"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="JN755_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7381333.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JN755_002576"
     CDS             complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JN755_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7381334.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDSGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="JN755_002577"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="JN755_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7381335.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="JN755_002578"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="JN755_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JN755_002579"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JN755_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7381336.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4022 a         4156 c         4236 g         3735 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatagc
//
LOCUS       DADLTI010000090        16062 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLTI010000090 DADLTI010000000
VERSION     DADLTI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.090"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1101
                     /locus_tag="JN755_002580"
     CDS             <1..1101
                     /locus_tag="JN755_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7381337.1"
                     /translation="EKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAI
                     KGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRA
                     ALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGER
                     ETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGN
                     RIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREV
                     MDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARL
                     RFGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1114..2073
                     /gene="accA"
                     /locus_tag="JN755_002581"
     CDS             1114..2073
                     /gene="accA"
                     /locus_tag="JN755_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7381338.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2172..4313
                     /gene="ldcC"
                     /locus_tag="JN755_002582"
     CDS             2172..4313
                     /gene="ldcC"
                     /locus_tag="JN755_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7381339.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4370..4759
                     /locus_tag="JN755_002583"
     CDS             4370..4759
                     /locus_tag="JN755_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7381340.1"
                     /translation="MLGLKQVHHIAIIATDYAVNKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4824..6122
                     /gene="tilS"
                     /locus_tag="JN755_002584"
     CDS             4824..6122
                     /gene="tilS"
                     /locus_tag="JN755_002584"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7381341.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6171..6431)
                     /gene="rof"
                     /locus_tag="JN755_002585"
     CDS             complement(6171..6431)
                     /gene="rof"
                     /locus_tag="JN755_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7381342.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6418..6618)
                     /locus_tag="JN755_002586"
     CDS             complement(6418..6618)
                     /locus_tag="JN755_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7381343.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6784..7317
                     /locus_tag="JN755_002587"
     CDS             6784..7317
                     /locus_tag="JN755_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7381344.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            7326..7748
                     /gene="arfB"
                     /locus_tag="JN755_002588"
     CDS             7326..7748
                     /gene="arfB"
                     /locus_tag="JN755_002588"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7381345.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7762..8472
                     /gene="nlpE"
                     /locus_tag="JN755_002589"
                     /gene_synonym="cutF"
     CDS             7762..8472
                     /gene="nlpE"
                     /locus_tag="JN755_002589"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7381346.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8627..9451)
                     /locus_tag="JN755_002590"
     CDS             complement(8627..9451)
                     /locus_tag="JN755_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7381347.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9504..11222)
                     /gene="proS"
                     /locus_tag="JN755_002591"
     CDS             complement(9504..11222)
                     /gene="proS"
                     /locus_tag="JN755_002591"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7381348.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11333..12040)
                     /gene="tsaA"
                     /locus_tag="JN755_002592"
     CDS             complement(11333..12040)
                     /gene="tsaA"
                     /locus_tag="JN755_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7381349.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12037..12441)
                     /gene="rcsF"
                     /locus_tag="JN755_002593"
     CDS             complement(12037..12441)
                     /gene="rcsF"
                     /locus_tag="JN755_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7381350.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12559..13374)
                     /gene="metQ"
                     /locus_tag="JN755_002594"
     CDS             complement(12559..13374)
                     /gene="metQ"
                     /locus_tag="JN755_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7381351.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13414..14067)
                     /gene="metI"
                     /locus_tag="JN755_002595"
     CDS             complement(13414..14067)
                     /gene="metI"
                     /locus_tag="JN755_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7381352.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14060..15091)
                     /gene="metN"
                     /locus_tag="JN755_002596"
     CDS             complement(14060..15091)
                     /gene="metN"
                     /locus_tag="JN755_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7381353.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15279..15854
                     /gene="gmhB"
                     /locus_tag="JN755_002597"
     CDS             15279..15854
                     /gene="gmhB"
                     /locus_tag="JN755_002597"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7381354.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3906 a         4032 c         4309 g         3815 t
ORIGIN      
        1 gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca
       61 gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt
      121 attggcgcca tcaaaggggt aggtgaaggt ccgattgagg ccatcatcga agcccgtaat
      181 aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac caaaaagtta
      241 aaccggcggg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacac
      301 cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa
      361 gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt
      421 gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt
      481 gaaacgttag gcctgtacct gaccggacac cctatcaacc agtatttaaa agagattgag
      541 cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc
      601 acagctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc
      661 ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc
      721 ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc
      781 agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac
      841 gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat
      901 gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca
      961 gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg
     1021 cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag
     1081 gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa
     1141 cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat
     1201 gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa
     1261 ctgacgcgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat
     1321 ccgcagcgtc cttataccct agattacgtt cgcctggcat ttgatgaatt tgacgaactg
     1381 gctggcgacc gtgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat
     1441 ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc
     1501 cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg
     1561 gctgaacgct ttaagatgcc tatcatcacc tttatcgaca ccccgggggc ttatcctggc
     1621 gtgggcgcag aagagcgtgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct
     1681 cgccttggcg taccggtagt ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg
     1741 gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg
     1801 ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca aagcgccgct ggcggctgaa
     1861 gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc catcatcccg
     1921 gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa
     1981 ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt
     2041 tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac
     2101 ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc
     2161 aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg
     2221 agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac
     2281 aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt
     2341 ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt aatgaatatc
     2401 tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc
     2461 ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta
     2521 tgcgtcagta caccaacgaa tatcttgata acattacgcc gccgttcacg aaagccttgt
     2581 ttacctacgt caaagagcgg aagtacacct tttgtacgcc ggggcatatg ggcggcaccg
     2641 catatcaaaa aagcccggtt ggctgtctgt tttatgattt tttcggcggg aataccctta
     2701 aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gcttgaccac accgggccac
     2761 acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta
     2821 ccaacgggac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta
     2881 cgctgttgat cgaccgcaac tgccataaat cactggcgca tctgttgatg atgaacgatg
     2941 tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattctgggg gggatcccgc
     3001 gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccactacg caagcacaat
     3061 ggccggttca tgcggtgatc accaactcca cctatgatgg tttgctctac aacaccgact
     3121 ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtata
     3181 ctcattttca tccaatctac cagggtaaaa gtggtatgag cggcgagcgt gttgcgggaa
     3241 aagttttctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcac
     3301 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
     3361 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc
     3421 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
     3481 aagaggtcca gcggctgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
     3541 cgcaggtgga tgaagccgaa tgctggcccg ttgcgcctgg cgaacaatgg cacggcttta
     3601 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgacaccgg
     3661 ggatggacga acagggcaat atgagtgagg aggggatccc ggcggcgctg gtagcaaaat
     3721 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
     3781 ttagtattgg catcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
     3841 agcgctctta cgatctcaac ctgcggatca aaaatatgct gcccgatctc tatgctgaag
     3901 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
     3961 ttcgtaaaca cgatctttcc ggtttgatgt tgcgggcatt cgatactttg ccggaaatga
     4021 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
     4081 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
     4141 cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac
     4201 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggcgcga
     4261 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
     4321 gagcggcttc tgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
     4381 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgaaca aagctttcta
     4441 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
     4501 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
     4561 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
     4621 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
     4681 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
     4741 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
     4801 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagaccactt ctcacctcac
     4861 gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
     4921 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
     4981 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
     5041 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
     5101 cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa gtgctggtca
     5161 ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg
     5221 gccctgccgg gctttcggct atggcggaag tttcggagtt tgctggaaca cggcttatcc
     5281 gcccgttgct tgcccgcacg cggggggaac tggagcagtg ggcattggcg catggtttac
     5341 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
     5401 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
     5461 ccacactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
     5521 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
     5581 gtcgcgcggc gattattcgc cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg
     5641 acgcgctggt gcgcatctgg caggaagtgg cgctggcgcg ggaagatgct tccccctgtt
     5701 tacgtttggg cgcctttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
     5761 tcaccgggca aagcgaaacc attgtgctgt ggcagacgtg gctacaaccg ctggaattac
     5821 cggcggggct gggaaccgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
     5881 acgaagcggt cagcgtgcgt ttcaaagcgc caggattgct gcatattgtc gggcgcaacg
     5941 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
     6001 ccacgccact gctgttttat ggagaaacgc tgattgcggc ggcaggggta tttgtgacgc
     6061 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
     6121 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa tcaggattcg
     6181 cttaccacca ccgtaccgat ttcagggtgg ctaaagctgg taattttatc cagacgcagc
     6241 tcgcgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
     6301 tcactggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
     6361 tgctggcagg cgagctcaag attatcgtaa tcatcacaat taattggttg atacgtatca
     6421 ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
     6481 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
     6541 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
     6601 ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt ggatgcaagt
     6661 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
     6721 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
     6781 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
     6841 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
     6901 atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
     6961 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
     7021 ttgtggattg agctggggct gccggatgag cgacgaatta agaaagcctg cacccaggcc
     7081 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
     7141 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
     7201 gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggt
     7261 gttatctggt tatcggatga taagaataat ctggaagtta acttaaccgc ctggtaacaa
     7321 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
     7381 ccattcgtgc gcagggcgct ggcgggcagc atgttaataa gacctcaacg gctattcatc
     7441 tgcgttttga cattcgggcg cccagcctgc cagagtatta caaagagcgt ctgctcgccg
     7501 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
     7561 gtcaggaact gaaccgcgaa gccgctctgg cccggttggt ggcagtgatt aaagatttaa
     7621 caaaagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
     7681 tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
     7741 gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
     7801 agcctcttta ctctgatggg atgtaataat cgggccgaag tcgatacgct ttctccggcg
     7861 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctacc gtgtgccgat
     7921 tgcgaaggaa tcgaaatctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
     7981 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
     8041 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
     8101 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat
     8161 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
     8221 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
     8281 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
     8341 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
     8401 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
     8461 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct
     8521 tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata
     8581 aggcgtttac gccgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc
     8641 caacaaagcg acccgccttg atataaatcc caggcttcag gtgcccaaca tactgtaact
     8701 gcgtttctgg cttaaacgca gagacatcac caatacgcat atgcatcaga tcggcagggc
     8761 taatccagcc cgattgcgcc agtgtaagtg gatgcccggc tttggcaaat gcatcagtga
     8821 caaattccga acaaaaccac gactttttgt ctccttcgca cacactgctt aactgcgctt
     8881 tcgccaggtc gctgacgcac tgttggcgaa aatcctcgga gaacggattc agtgagcaca
     8941 tctggcgagt caccataaag ggaataaatt cgacaatgtt gcgatagtta taaccgctat
     9001 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
     9061 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acaatctgga
     9121 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag aaagattgca acgtgactca
     9181 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
     9241 gcaaatcgcc gggtttgaga tcaggtgccg ttatttcttt tattgattgt tcggtgaaag
     9301 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
     9361 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
     9421 gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaagggccct
     9481 gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg
     9541 tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg
     9601 ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca
     9661 gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca
     9721 cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc
     9781 atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat gccgcgttcg
     9841 tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc
     9901 atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag
     9961 tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta
    10021 ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg
    10081 gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat gttagcacca
    10141 gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt
    10201 accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc ttcggtcgcg
    10261 aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga
    10321 tcaccgcgca ccagcagcgc aaccagcggg aagctactgc cttcaaccgc tttaaccagc
    10381 agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt
    10441 ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc
    10501 ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc
    10561 acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata
    10621 gaaccggtgt cggcctgtac ggcgcggaaa tccagcccca tgcggctgaa gattttgctg
    10681 taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag
    10741 taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg
    10801 tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc
    10861 tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc
    10921 tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct
    10981 tgccacaaat cggctggctg aaccaccggc atcgacacct cgatcgcacc ggcgttgttc
    11041 atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag
    11101 gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg
    11161 atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc
    11221 atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa
    11281 agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg
    11341 gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat
    11401 gtagccagac ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgcgggt
    11461 cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca
    11521 aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt
    11581 gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat
    11641 ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt
    11701 tatggcaaac aacctctttc agctctacca gcgacatgcc aattgggtta gggcggaaag
    11761 tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag
    11821 tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga
    11881 atgcttccag gccgcgaacg gcgtcggcct ggttgtagga agcaatgaga tgcagttcgc
    11941 cgttggcgct ttttaccaga cctggctggc gcggaacggc gaatttttct ttatagggcg
    12001 agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc
    12061 agagccgata catacagcct gacgatagca gcccggcgta ccgctggtga cttcgcagct
    12121 atgcagtaat acagcattgg ctttcatttt agaggcattg atttgcatct gcttacgtgc
    12181 ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc
    12241 accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg
    12301 cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg
    12361 aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa
    12421 acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa
    12481 tcaggagctt gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt
    12541 gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc
    12601 tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc
    12661 tttgttatct tcacgcgtta cgatcaggtt tacgtatggg gagtctttat cttcgacaaa
    12721 gataccgtct ttcgccggag tcaggccaat ctggctggca taggtggtat tgataactgc
    12781 cagagcgatt tgcgcgtcgt ccagagaacg cggcagttgc ggtgcttcca gttcaacaat
    12841 tttcagattt tttgggttct caacaacatc aagaactgtc ggcagcaggc caacgccatc
    12901 tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg
    12961 atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt
    13021 ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg
    13081 atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc
    13141 tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag
    13201 gccatatttg tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac
    13261 aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc
    13321 cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta
    13381 ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat
    13441 gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt
    13501 catcaccgtc gcgttgtagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc
    13561 ggcaccgact gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc
    13621 cgcattcacc agacccggca acgcttccgg taacagcact ttacggacga tctgcattgg
    13681 cgtggcaccc attgcgcggg aagcttcaat taacccggtt gggatctcca gcagagcgtt
    13741 ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa ttgctgcctg
    13801 caaaccaatc gatgtaccga caataacgcg ggtaaacgga atcatccata ccagcaagat
    13861 aatgaacggg atggaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc
    13921 gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat
    13981 cacaaaccca aaaaatccgg atacgaaggt cattgccagc gtttcccata cgccacgaac
    14041 cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct
    14101 gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga
    14161 tgccgaattt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt
    14221 tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg gtaaactcca
    14281 gacgcagcat cggcacgcag tcagtaaatg gctccgtttg cagacgttcc tggtaatctt
    14341 ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat
    14401 gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca
    14461 cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac
    14521 ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca
    14581 gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa
    14641 tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc
    14701 caagaccaac caatgacagc aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt
    14761 tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa
    14821 aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg
    14881 tggtcaattc ctggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta
    14941 cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc
    15001 cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga
    15061 acactttggt gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt
    15121 cgtagcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc
    15181 cagacgtcta aatcaatcag gtttatgcga agagcacttt cttgcaggtc gaaacatgcg
    15241 atactagcgt cccatgcctt attaaggagc tataaaaggt ggcgaagagc gtacccgcaa
    15301 tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca
    15361 actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg
    15421 cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt
    15481 ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg
    15541 gtatctatta ttgcccgcat catccgcagg ggagtgttga agagtttcgc caggtctgcg
    15601 attgccgcaa accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata
    15661 tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg gttgcggcga
    15721 acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg
    15781 cggcagattg ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa
    15841 aaccggcgca atgattaaaa gatgaacggt tgaaataaaa atgcattttt ccgcttgtct
    15901 tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca
    15961 caaacagccg gttcggttga agagaaaaaa tcctgaaatt caggggtgac tctgaaagag
    16021 gaaagtgtga tatacgccac ctcgcgacag tgagctgaaa gc
//
LOCUS       DADLTI010000091        15952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLTI010000091 DADLTI010000000
VERSION     DADLTI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.091"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JN755_002598"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JN755_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7381355.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JN755_002599"
     CDS             complement(998..2020)
                     /locus_tag="JN755_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7381356.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JN755_002600"
     CDS             complement(2034..3536)
                     /locus_tag="JN755_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7381357.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSEKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JN755_002601"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JN755_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JN755_002602"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JN755_002602"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7381358.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JN755_002603"
     CDS             complement(5853..6194)
                     /locus_tag="JN755_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7381359.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JN755_002604"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JN755_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7381360.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JN755_002605"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JN755_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7381361.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JN755_002606"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JN755_002606"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7381362.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JN755_002607"
     CDS             12873..14216
                     /locus_tag="JN755_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7381363.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JN755_002608"
     CDS             complement(14278..14484)
                     /locus_tag="JN755_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7381364.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JN755_002609"
     CDS             14809..15363
                     /locus_tag="JN755_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7381365.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JN755_002610"
     CDS             complement(15426..>15952)
                     /locus_tag="JN755_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381366.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3798 a         4373 c         3919 g         3862 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttttcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct
     3001 tgggtgttga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 atgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agccaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 cccaggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gacgaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcacat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADLTI010000092        15911 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLTI010000092 DADLTI010000000
VERSION     DADLTI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.092"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..396)
                     /locus_tag="JN755_002611"
     CDS             complement(<1..396)
                     /locus_tag="JN755_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7381367.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="JN755_002612"
     CDS             complement(393..3077)
                     /locus_tag="JN755_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7381368.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="JN755_002613"
     CDS             complement(3169..3669)
                     /locus_tag="JN755_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7381369.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="JN755_002614"
     CDS             complement(3699..4415)
                     /locus_tag="JN755_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7381370.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="JN755_002615"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="JN755_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7381371.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="JN755_002616"
     CDS             5832..6494
                     /locus_tag="JN755_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7381372.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="JN755_002617"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="JN755_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7381373.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="JN755_002618"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="JN755_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="JN755_002619"
     CDS             7420..7725
                     /locus_tag="JN755_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7381374.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="JN755_002620"
     CDS             7728..8132
                     /locus_tag="JN755_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381375.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="JN755_002621"
     CDS             8122..8421
                     /locus_tag="JN755_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381376.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8517..8999
                     /locus_tag="JN755_002622"
     CDS             8517..8999
                     /locus_tag="JN755_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7381377.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            9069..10055
                     /locus_tag="JN755_002623"
     CDS             9069..10055
                     /locus_tag="JN755_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7381378.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="JN755_002624"
     CDS             10348..10716
                     /locus_tag="JN755_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7381379.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /locus_tag="JN755_002625"
     CDS             complement(10923..11819)
                     /locus_tag="JN755_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7381380.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="JN755_002626"
     CDS             11924..12625
                     /locus_tag="JN755_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7381381.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="JN755_002627"
     CDS             12648..12812
                     /locus_tag="JN755_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381382.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="JN755_002628"
     CDS             complement(13024..14316)
                     /locus_tag="JN755_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7381383.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..15675)
                     /locus_tag="JN755_002629"
     CDS             complement(14344..15675)
                     /locus_tag="JN755_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7381384.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4145 a         4043 c         3682 g         4041 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
    15721 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
    15781 atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataaca gagatggata
    15841 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
    15901 aaaaaaaggt g
//
LOCUS       DADLTI010000093        15877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLTI010000093 DADLTI010000000
VERSION     DADLTI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.093"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..613)
                     /locus_tag="JN755_002630"
     CDS             complement(<1..613)
                     /locus_tag="JN755_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7381385.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTA"
     gene            complement(610..1872)
                     /locus_tag="JN755_002631"
                     /pseudo
     CDS             complement(610..1872)
                     /locus_tag="JN755_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1869..2777)
                     /locus_tag="JN755_002632"
     CDS             complement(1869..2777)
                     /locus_tag="JN755_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7381386.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2943..3740
                     /locus_tag="JN755_002633"
     CDS             2943..3740
                     /locus_tag="JN755_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7381387.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3791..4443)
                     /gene="pphB"
                     /locus_tag="JN755_002634"
                     /pseudo
     CDS             complement(3791..4443)
                     /gene="pphB"
                     /locus_tag="JN755_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4549..7110)
                     /gene="mutS"
                     /locus_tag="JN755_002635"
     CDS             complement(4549..7110)
                     /gene="mutS"
                     /locus_tag="JN755_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7381388.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7186..7365
                     /locus_tag="JN755_002636"
     CDS             7186..7365
                     /locus_tag="JN755_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381389.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7397..7750
                     /locus_tag="JN755_002637"
     CDS             7397..7750
                     /locus_tag="JN755_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7381390.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7787..9865)
                     /gene="flhA"
                     /locus_tag="JN755_002638"
     CDS             complement(7787..9865)
                     /gene="flhA"
                     /locus_tag="JN755_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY7381391.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9939..10949)
                     /gene="hypE"
                     /locus_tag="JN755_002639"
     CDS             complement(9939..10949)
                     /gene="hypE"
                     /locus_tag="JN755_002639"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7381392.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10946..12067)
                     /gene="hypD"
                     /locus_tag="JN755_002640"
     CDS             complement(10946..12067)
                     /gene="hypD"
                     /locus_tag="JN755_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7381393.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12067..12339)
                     /gene="hypC"
                     /locus_tag="JN755_002641"
     CDS             complement(12067..12339)
                     /gene="hypC"
                     /locus_tag="JN755_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7381394.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12330..13202)
                     /gene="hypB"
                     /locus_tag="JN755_002642"
     CDS             complement(12330..13202)
                     /gene="hypB"
                     /locus_tag="JN755_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7381395.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13206..13556)
                     /gene="hypA"
                     /locus_tag="JN755_002643"
     CDS             complement(13206..13556)
                     /gene="hypA"
                     /locus_tag="JN755_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7381396.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13768..14229
                     /gene="hycA"
                     /locus_tag="JN755_002644"
     CDS             13768..14229
                     /gene="hycA"
                     /locus_tag="JN755_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7381397.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14354..14965
                     /locus_tag="JN755_002645"
     CDS             14354..14965
                     /locus_tag="JN755_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7381398.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14962..>15877
                     /locus_tag="JN755_002646"
     CDS             14962..>15877
                     /locus_tag="JN755_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY7381399.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGI"
BASE COUNT         3623 a         4251 c         4204 g         3799 t
ORIGIN      
        1 ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct
       61 ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat
      121 gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc
      181 gtttatcgcc cggtcggtaa tagggcacca gcgggacatt tcccatccgc atcaccacgt
      241 atggtgtgaa cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcgccg
      301 tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg
      361 ccagatgaaa gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg
      421 cgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca
      481 gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc
      541 gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg
      601 cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa
      661 gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc
      721 gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc
      781 ggaggtctca ccacctgcga caataaagcg tgtcatgcct tccgctgcta accgcgccgc
      841 tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg
      901 aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc
      961 ctggcccaga acccactctg ccagttcgtg agcataagcg gccagagttt cggttgagag
     1021 gcagcgtgcc acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctagcg
     1081 gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga gcggacgccc
     1141 tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag
     1201 accagaaccg cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg
     1261 gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag
     1321 ctcttgacga acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg
     1381 ccctgtggat tgcgcttcaa ccagacgggg aagatagctg ttggtcatgg gatttaccgg
     1441 gtgatggcgc atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata
     1501 aaccgtacgt ccgttgaccg gcagggccgg agagaagacc gtaaacggcg tgtcgagagc
     1561 atccattaat gcatcggtaa ccgggccaat attacctttc gccgtactgt cgaaagtaga
     1621 gctgtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag
     1681 cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga tcaccagtgc
     1741 gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc
     1801 gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc
     1861 aatcttgatc atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg
     1921 ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt caatgctgtt
     1981 gaggccaatg gcagcgggaa gtgcagggct ttggctgtat cggcaaccag accaagatcc
     2041 ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc
     2101 cggttttcga acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc
     2161 agcgggatcc ccgcacgggc tgcaagtgcc atcgcttcgg ctccggcagc aatatgtacg
     2221 cccgctaaca actggtgaat aatttttacg gtcgaaccta gtcccggttc tgcacctatg
     2281 cgataaactt ttccggcaac ggcttccagc acgggtgcca gtcgttcaaa ggcaatatcg
     2341 ctaccggagg ccatgacagt catttcaccg ttagcggctt ttactgcacc accagaaact
     2401 ggcgcatcca gcatttccag atcgaatcca gccagagcgg tagcaatttc ttgcgcatca
     2461 gcactagcga tagtggaaga aaccattact gccgtaccgg gtttcagatg ttgtgcaacg
     2521 cctgtttcac caaacagcac ctgtttaacc tgggccgcat tgaccaccag caccagcaga
     2581 gcgtccagtt tttcggcaaa cgtcgcggcg ttatcagaaa ccccgcaagc acctgcctct
     2641 ttcaacgtag cgcaggcatt gctgttcagg tctgcgcccc aggtagaaaa acctgcgcgg
     2701 acacatgaca gtgctgctcc cattcccatt gaccctaagc caacgatacc gacatgaaac
     2761 tcagatcccg ttttcatctg ctctccttgt taatttaagt gatattttgt ttgacattgt
     2821 gaatataagc gctgggagat aacgatatgg tgagctgatt cacataaatt aacattttgt
     2881 gttattttat gtgaactaag cgttagttgc gccgcgcacg tttcgcaggc aaatagcgta
     2941 gaatgtcagc aggacaaagg aaggagcaaa agttgatacc cgtagagcgt cgccaaatca
     3001 tccttgagat ggtagctgaa aaaggcattg tcagtattgc tgaactaacg gacagaatta
     3061 atgtgtcaca tatgaccatt cgtcgggatt tacaaaaact ggagcagcag ggagccgttg
     3121 tgctggtgtc cggaggcgtc cagtctccgg gacgcgtggc gcatgaacct tctcatcagg
     3181 taaaaactgc gctggcaatg acgcaaaaag cggctattgg caagctggcg gcaagtcttg
     3241 ttcagccggg aagttgtatc tatctggatg cgggaacgac cacgttagcg atagcacagc
     3301 atctgattca catggagtca ctgactgtgg tcacaaacga tttcgttatt gcgaactact
     3361 tgctcgacaa cagtaattgc acaattattc acactggcgg tgcagtgtgt cgggaaaacc
     3421 gttcctgtgt cggggaagcc gctgcgacca tgctgcgcag cctgatgatt gatcaggctt
     3481 ttatttctgc atcgtcatgg agtgtgcggg ggatttctac gccagcggaa gataaagtca
     3541 cggtgaaacg ggcgattgcc agtgctagcc gccagcgagt tttggtctgt gatgcgacga
     3601 aatatggtca ggtggcgaca tggctggcgt taccattaag cgagtttgat cagattatta
     3661 cagacgacgg tttgccggag agtgccagtc gcgcgctggc gaagcaggat ctctctttgc
     3721 tggtagcgaa aaatgaataa tggcctgcaa taacatctgg ttactcatgc ttcacagaag
     3781 aagcatgaga ctaccttatt ttataaaatg acagccgccc acttttcggc gatccggtat
     3841 caatataaat ctggttagcg aacgtctgaa tgttatcaaa catcatatgt ccaaatataa
     3901 aataatcagc gccgtttatt tgttgtaact cgccattaag cgatttctgc acacgataaa
     3961 caggccagag taattcgctc tccgctattt ctttgtcaaa gagatattca tcacccggat
     4021 aatctgcatg tgcgatgaca tattttatta tgtcgttagt gatttcaata atatgtggaa
     4081 ggtgatggaa tttcagcaac agatctattg cctcttgttg caatcattta aatcgaaaaa
     4141 ccagtcacca ccgctggcaa gccacatatt gccatcgccg gtcgcgaacg catccagcgc
     4201 cattgcttcg tggttgcctt taacggagat aaaccagggc tggtttagca ggcgcaggac
     4261 gtcaagactc tccggcccac gatcaatatt atcgccgaca gaaataagta agtcggtttc
     4321 ggggaaaaaa gagagttgat gtaagcggga ttgtaataac tgatattcac catgaatatc
     4381 accaacgacc catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg
     4441 catggtttta ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt
     4501 cagttgtcgt taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact
     4561 cttcaagcga taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt
     4621 ttccagtgct tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt
     4681 accatccact tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg
     4741 ccgtgcgcgc ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta
     4801 gcttttgctc gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc
     4861 cagcgcatcg agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc
     4921 gaaatagtgg gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca
     4981 cgcccacgcc agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa
     5041 caccagactg tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat
     5101 aaaggttgag cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc
     5161 gataggtcca atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc
     5221 aatcagtgcg gtctggcgca tataggtact tttaccgccc atgttcgggc cggtaataat
     5281 caacatacgg cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac
     5341 ttgttcaact accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt
     5401 cgggcaggtg tagttcaggg tataggcccg ttccgccagg ttaaccagca cgtcgagttc
     5461 cgccagcgcg ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacag
     5521 ctcttcataa agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc
     5581 ttcgtactct tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg
     5641 catgtagttg atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg
     5701 caccgcatta aagccaactt ttagcgtgtc caggccggta cgttcacgct cgcggacttc
     5761 cagacgctcc agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc
     5821 gttatagccc gatgcgataa caccaccgtc gcgtaacagc accggcggtg tgtcgatgat
     5881 tgctcgctcc agcagatcgc gcagctcggc aaactctccc atcttctcac gtagcgcctg
     5941 taccggtgca ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc
     6001 gtggcgcata cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat
     6061 acgttccagg tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa
     6121 tgcgccaata gtttgctggc gctcaagcaa cacgcgagta tcgcgcactg gcatatgcag
     6181 ccagcgtttc agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag
     6241 cgtattttcc gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc
     6301 atccataatg atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt
     6361 cgtgcgttgg gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg
     6421 cgcgttctcg acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg
     6481 gcgcgcggtg tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc
     6541 aatcagcgac atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg
     6601 cagctctgcc gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct
     6661 gatatccagc gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt
     6721 gtcctgacgc tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac
     6781 aactttgcgc tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc
     6841 aacggactct ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat
     6901 ccccgccatc gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag
     6961 cagttgcgac gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta
     7021 aaacagcagg atctcgggat gctgggcttt cagcttgaga tactgctgca tcatgggcgt
     7081 atgggcgtcg aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat
     7141 ggggtgtgat ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta
     7201 atcatttcag gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg
     7261 ttttgcagct tttgcgtgat gcttatgaag caagtacgca aaattgtatc gattggcgaa
     7321 cccttcttct ttagagtcat agtgcccttt aagattaaga tgtaatataa atgcatctta
     7381 atgaggtgga ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa
     7441 aatgatcgat ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga
     7501 ggcgttgttt gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc
     7561 agcctgtaag cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca
     7621 gattatgcgt tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca
     7681 tctcatcgat gacaagcgtc cggtgccaga actgccggaa aaatatcgtc cgaaaaaacc
     7741 tcgtgaatag tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg
     7801 atttatcaat tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac
     7861 gttgcgcagc gcctttaggc ccggcaacca cgctgttggt ttctttcagc acgcgcacaa
     7921 tcaactgata ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag
     7981 gctccggtaa agcaatatct ggcaatgaca gctgcagcac gttgccgcgt gttagcaata
     8041 ctgcgcgctc aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca
     8101 aggtgcgcag cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt
     8161 tgaaggtaaa ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca
     8221 ggtgaatcgg gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga
     8281 ccattttttt cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt
     8341 tgttgctgcc gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta
     8401 actccagtgg catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa
     8461 aacgaccaat gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat
     8521 cgctttccag caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat
     8581 tacgcccact gagattatgg atcgcacggg caatcagctc tttacccgtg ccagtttcac
     8641 cgaggatcag cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt
     8701 acatggcttc gctgcggcca ataatctcgc caaattcact atcaacattg ttgagttgct
     8761 cggtcagggc gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg
     8821 cgagggcgtt atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat
     8881 tggtagtggt aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt
     8941 cgccagacat cagcggtaac aggcacaagg tttgaatctg gttgccccag gtgtcgaaca
     9001 acatgcgttc atagggggct aaatcgtccc gctcgtagag attgatcagc agcatctctt
     9061 tacttttgaa cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg
     9121 cgggatgttg tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc
     9181 tgcgtaagac aatgctgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt
     9241 cgctgaccag ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga
     9301 tgcggaagtt atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc
     9361 tctggatttg ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact
     9421 ctttttcgct ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc
     9481 ctcccgccgc cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcgg
     9541 ccagctgcgg ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg
     9601 acaaaatgct gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggg
     9661 tgtctttttc acgcgacgcg taataagacg cacgttgagt ctgcgcttgc cacaacacaa
     9721 tagccgcgtt gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc tcacacagcg
     9781 aggccagatc gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct tgctgtccga
     9841 gatcactcat cggtgtatat gacattttgt tcttccagga aagcaccgca aagcggtgca
     9901 aaaggtcatt gactaactat aggagattta gaattttatt agcatatacg cggaagcggt
     9961 tcggcgtgtg gtaaatcgag ggttcgtttc acgccataca gaccggcaag acgaacacct
    10021 ttacgttcca ccacttcacc aatcagcgcc gcgtctttcc ccagtggatg ggaatgtaac
    10081 gctgccagca cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt accttcgttg
    10141 gcaaagttca gggcgtccag tcccagcaat tcgcaaacgc cgcgcacggc aggtttaacc
    10201 ggcagcgctg attcagaaat ttcaatacca caaccgcagg ctgccgcgaa ctcatgaacc
    10261 accgcgttta caccaccacg ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc
    10321 agcgtctgaa taagcggcgt cagcaccgcg cagtcgctga ccagttcgcc atccagcccc
    10381 agctgctcac gcaggttaag gatagtcgcc ccgtggtcgc cgagtgtacc gctaaccagc
    10441 aatacatcgc ctgcggttag cgtctgtgcg ccccagtgaa tattcgccgg aattgcgccc
    10501 atgccagcgg tgttgataaa cagtttatct gccgcgccgc gctgcaccac tttagtatcg
    10561 ccagtaacga tggcaatgcc tgccgtgcgg gcggtttctg ccatgctggt cactgcggct
    10621 ttcagtgtct ccatcggcaa tccttcttcg aggataaagc cacaggagag atagcgcgga
    10681 atagcgccac tgaccgcaac gtcattcgcg gtgccgcaaa tcgccagctt gccgatatta
    10741 ccgccaggaa agaacagcgg gtcaatgacg taactgtcgg tggagaacgc cagacggtcg
    10801 ccttccgcta ccagctgcgc cagatcaaga cgtgcctgat cttcctgctc tgccagccac
    10861 gggttggcaa aggcttccat aaacaggctg ttgattaatt gctgcatcgc ctggccgccg
    10921 ctgccgtggg cgagttggat attattcacg cttcactctc ctgctgacga tactgatacc
    10981 acgcggcgca cgctccttcg gaggagacca tcagtgcacc aaatgcggtt tgaggattac
    11041 aggtgttacc aaacagcggg cattgatgcg gcttacattt gcccgttaat acctcaccac
    11101 aacgcgcgcg cgggtcatcg cagacctgct gcggtgccgg gcggaaatgt gcttcggcat
    11161 cgaatcgttg ataatccggc gtcaggtgca cgccagaaga ttcaatcacg cctaagccgc
    11221 gccattcgct gtcgccgttg acacagaaca catcggcaat cgcctgttgc gccagcaggt
    11281 taccggcatc cggtaccacc cgacgatact gattctctac cttgctgtgg gccgctattt
    11341 tctgctccac cagcatgacc acgccttgca gtagatcaag gggttcgaaa ccagcgacca
    11401 ccagcggacg atgaaaatcg ctggcgataa aattataggc atcagtgccg ataaccatac
    11461 taacgtggcc tggcgcgagg aacgcgtcga taccgttatc cggctgttcc agcaaactgc
    11521 gcagcgtcgg gataagcgta atgtgctggc agaagaagta aaaattctgc acatcacgcg
    11581 ctttcgcctg ttgcagagtg atggcggtgg tcggcatggt ggtttcaaaa cctaagccga
    11641 agaacaccac tttgcgggtt ggattctcct gcgccagttt caacgcatcc atcggcgagt
    11701 aaacgatgcg cacatcggca ccgcgtgctt ttgcctgcaa cagcgatccc tgtttccctg
    11761 gtacgcgcat ggcgtcgcca aaggtacaga agatgacttc cggatggctg gcaatctcca
    11821 cgcaggtgtc gattctaccc atcggcagta cgcacaccgg gcaacccgga ccgtggataa
    11881 actcaacgtt ttcaggcagt aactggtcga ggccgaattt aaaaatggcg tgggtatgac
    11941 cgccacacac ttccataatc cgcagagggc gttcggcggt gtaagagaga tgtgaagcac
    12001 gttcgcgcag atgctcaatt aactgcatca cctgttccgg cgcgcgatat tcatcaacaa
    12061 aacgcattat ttttcctcgc catacaacag cgcaccgaca tccggctcaa cgtcaaacat
    12121 gttttgtaag gcgtcgagag tgtcgcgtgc ttcggcttca ttaattacgc tcatggcaaa
    12181 gccaacgtga accagtaccc actggcccac gcgcggctga ccgttttcat cgcagctgcc
    12241 gactaacgtt aaatcgacat cgcgctgaat gccgcagacg tcgactttcg cctggttgcc
    12301 gtcaatggtg cggatctggc cgggaacgcc tatgcacatc gctgtgtctc cagccagttc
    12361 agccactggt ccatcccttc gccgctggtg gcggaaataa ggatgatttc aatttctgga
    12421 ttgacttcgc gggcgcaggc gatgcacttc tcaacgtcaa agttgagata cggcaacagg
    12481 tcaactttgt tgagcagcat cagcgaggcg gcggcaaaca tatgcggata tttcagcggt
    12541 ttgtcttcac cttcggtaac ggaaagcacc gctactttgt gtttttcacc gagatcgaag
    12601 ctggccgggc atacgaggtt gccaacgttt tcgataaaca gaataccgtt atcgtccagt
    12661 ggcagacgcg gtgcggcgtc ggcaatcatc tgtgcgtcaa gatggcagcc tttaccggtg
    12721 ttcacctgaa tcgctggtgt accggtagcg cgaatgcgtg cggcatcgtt cacggtttgc
    12781 tggtcgcctt caataaccgc gcacggaacg ctgtctttca ggcgcattaa ggtttccgtc
    12841 agcagggtgg ttttaccgga accagggctg gaaaccaggt tgagcaccag ttgcttgcgg
    12901 gcagcaaagc gcgcgcggtt gcgttcagcc agacggttgt ttttgtccag cacgtcaatt
    12961 tcgacttcca gcatccgacg ctggctcatg cccggtgtgt gagtgcccgc ttcgccatga
    13021 ccgtaatgca ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg
    13081 gtgattttca tctgcgggcg tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga
    13141 gggttatgtt catcaccctc gatatacagg ttgccttcac cgcaaccgca tgttgtacac
    13201 atcgctcact cctggtctat ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc
    13261 tgtagcatgt caccatgaca ctgtggacag cggcggacgc gctgggtcag tagcgtcaca
    13321 tactgttggc atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa
    13381 ccttccgcca cgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg
    13441 acacaagaaa atgcgccaat tttgagccag accccagtta cgcgttttgc gccgtgtttt
    13501 gcggcctgct gttcgatcaa ttccagtgcc cgttggcaga gggttatttc gtgcatatcg
    13561 cctcccatta actattgcca gctacaagca ataattgcgc cagtgttgat tatccctgcg
    13621 gtgaataatg tcgatgatgt cgaaatgaca cgtcgacacg gcgacgaaat tcatctttag
    13681 cttaaaaatc tctttaataa caataaatta aaagttggta caaaaaatgc ttaaagctgg
    13741 catctctgtt aaacgagtaa cctgacaatg actatttggg aaataagcga gaaagccgat
    13801 tacatcgcac agcggcatcg tcgcctacag gaccagtggc acatctactg caattcgctg
    13861 gttcagggga tcacgttatc gaaagcgcgc ctgcatcacg ccatgagctg cgcgccggac
    13921 aaagaactct gtttcgtcct ttttgaacat tttcgtattt acgtcaccct ggcggatggc
    13981 tttaacagcc acaccatcga gtattacgtc gaaacaaaag atggcgaaga caaacagcgg
    14041 attgcgcagg cgcaactgag cattgacggc atgattgatg gcaaggtcaa catccgcgat
    14101 cgcgaacagg ttctggaaca ctatctcgaa aaaatcgctg gcgtttacga cagcttatac
    14161 accgccattg aaaacaatgt gccggtgaat ttaagccaac tggtaaaggg acaaagcccg
    14221 gcagcatgag ctgaggcttt gcccgttttg caggcgttac gcctgtttgg ggatgggcgt
    14281 gtcgatgagt gtcgaaaatg acatttcatc ggcatgtttt cgtcaaaaat gacaatcacc
    14341 tgaggaatgc ctggtgaatc gttttgtaat tgccgactcc acgctctgta tcggctgcca
    14401 cacctgtgag gccgcctgtt cagagacgca tcgccagcac ggcctgcaat caatgccgcg
    14461 cctgagagtg atgctcaatg aaaaagaatc tgcgccgcag ctctgtcacc actgtgaaga
    14521 tgcaccctgc gcggtggtct gcccggttaa cgccatcacc cgcgttgatg gggccgtgca
    14581 gttgaatgaa agtctgtgcg taagctgcaa actgtgcggc atcgcctgcc cgtttggcgc
    14641 aattgaattt tccggcagcc gtccgctgga tattccggca aacgccaata ccccgaaagc
    14701 gccaccggca ccgcctgctc cggcgtgtgt cagcacattg cttgactggg tgccaggtat
    14761 tcgcgcgatc gccgtcaaat gtgacctttg tagctttgat gaacaaggtc cggcctgtgt
    14821 gcggatgtgc ccgactaaag ccctgcatct ggtggataac accgatatcg cccgcgtcag
    14881 caaacgtaag cgtgagctga cttttaacac ggactttggc gatctcacct tgtttcagca
    14941 ggctcaaagt ggagaggcta aatgagcgca atttccctga tcaatagcgg cgtggcatgg
    15001 tttgtcgccg ccgctgttct ggcatttctc ttttcttttc aaaaagcgct aagtggctgg
    15061 atagccggaa ttggcggcgc ggttggtagt ctgtatacgg cagccgcggg cttcactgta
    15121 ctgactggct cggttggcgt gagcggtgcg ctgtcgctgg taagctacga tgtgcaaatc
    15181 tccccgctta acgcgatttg gctgattacc ctcggtcttt gcggtctgtt tgtcagcctc
    15241 tataacattg actggcatcg ccacgcgcag gtgaagtgca acggcttgca gatcaatatg
    15301 ttgatggctg ccgccgtctg cgccgtcatc gccagcaacc tcggcatgtt cgtggtaatg
    15361 gccgaagtca tggccttgtg tgcggtgttc ctcaccagca acagcaaaga gggcaaactg
    15421 tggtttgcgc tggggcgtct tggcactctg ctgctggtga ttgcttgctg gctgctgtgg
    15481 cagcgttatg gcacgctgga tctgcgcctg ctggatatgc gtatgcaaca gctgccgctc
    15541 ggttccgata tctggctgct cggttccgat atctggctgc tcggtgtgat tggctttggc
    15601 ctgctggctg ggattattcc gctgcacggc tgggtgccgc aggcacatgc taacgcctct
    15661 gcgccagctg ccgcgttgtt ttctacggta gtcatgaaaa ttggcctgct gggcatgtta
    15721 accctgtcac tgctgggcgg taatgcaccg ctgtggtggg ggatcgcgct gctggtgctc
    15781 ggcatgatca ccgcgttcgt cggtggtctg tatgcgttga tggaacacaa catccagcgc
    15841 ctgctggctt accacaccct ggaaaatatc ggcatta
//
LOCUS       DADLTI010000094        15828 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLTI010000094 DADLTI010000000
VERSION     DADLTI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.094"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..880)
                     /locus_tag="JN755_002647"
     CDS             complement(<1..880)
                     /locus_tag="JN755_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7381400.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAG"
     gene            987..1283
                     /locus_tag="JN755_002648"
     CDS             987..1283
                     /locus_tag="JN755_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7381401.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JN755_002649"
     CDS             complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JN755_002649"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7381402.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JN755_002650"
     CDS             complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JN755_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7381403.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JN755_002651"
     CDS             complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JN755_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7381404.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4296..4379
                     /locus_tag="JN755_002652"
     CDS             4296..4379
                     /locus_tag="JN755_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7381405.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JN755_002653"
     CDS             complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JN755_002653"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7381406.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5857..6699)
                     /locus_tag="JN755_002654"
     CDS             complement(5857..6699)
                     /locus_tag="JN755_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7381407.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6902..8944
                     /gene="prlC"
                     /locus_tag="JN755_002655"
     CDS             6902..8944
                     /gene="prlC"
                     /locus_tag="JN755_002655"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7381408.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8952..9704
                     /gene="rsmJ"
                     /locus_tag="JN755_002656"
     CDS             8952..9704
                     /gene="rsmJ"
                     /locus_tag="JN755_002656"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7381409.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JN755_002657"
                     /pseudo
     CDS             complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JN755_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JN755_002658"
     CDS             complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JN755_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7381410.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12353..12688
                     /gene="uspB"
                     /locus_tag="JN755_002659"
     CDS             12353..12688
                     /gene="uspB"
                     /locus_tag="JN755_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7381411.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JN755_002660"
     CDS             complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JN755_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7381412.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14490..15689
                     /locus_tag="JN755_002661"
     CDS             14490..15689
                     /locus_tag="JN755_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7381413.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3981 a         4048 c         4154 g         3645 t
ORIGIN      
        1 cgccggcact gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca
       61 gaacagatgc cagagggata accgcctcag cccggatata cagcggaata ccgacaacgg
      121 cgcttaatgg gatggcgaag ggattatctg ctcctgcatg agcggcaatc cacgctgagg
      181 gaataaagcc ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat
      241 ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag
      301 ctttggtact gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac
      361 aactcacttt tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat
      421 ttgctgatga gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa
      481 tactggcaag aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac
      541 caaaggtcat ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa
      601 aagtgagagt cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg
      661 tcgagcaact acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc
      721 cttttctggc ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc
      781 tgacaccggc actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca
      841 tctctgcggc atcttgcagc atcgcaagcc aactactcat acgtaacctc attgggtaaa
      901 tctatatttc cagaataatc gaaatgaaat tcagatcaag atatttctgg tattatcgaa
      961 atatcaacca gacagagggg tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag
     1021 agctaggtca cccgacccgt ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag
     1081 ggttgccggt cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc
     1141 atatatcagc actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga
     1201 tgtgtgtttc gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct
     1261 gcgtaaatag caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa
     1321 tttgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc
     1381 gcactttacc aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc
     1441 atcaaccact ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat
     1501 ttccagcacc acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg
     1561 gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt
     1621 atcttccgca aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac
     1681 ggaaatcccc atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag
     1741 ataatggata atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga
     1801 cgtgccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa
     1861 agtgaaagag agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat
     1921 gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac
     1981 gtgcagccag agcagcgttg ccaggctacc aatcggggta attttcggcc ccaaatcgca
     2041 gccaatcacg ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc
     2101 aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc
     2161 ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag
     2221 tacgccagaa agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat
     2281 gccgagcgag aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat
     2341 cgcatgaccg cgtttagcga cgacaaataa tatcagtgcg cccacagctg caatggcgct
     2401 caccggaatg ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac
     2461 ccagccagtt ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc
     2521 catatcgtag ttctgcggaa tatctttgcg aaaatagaga tgtagcatca ccagcgtggc
     2581 aacaatcgcg gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc
     2641 aaagaaatca gcggaaacga tattcaccaa gttggagaca ataagcggca ggctggcggt
     2701 atcggcaatg aatccggccg ccatcacgaa cgccagcgtc gtgcctttac tgaatcctaa
     2761 agccagcagc atggcgatga caatcggcgt caaaataagc gccgcgccat cattggcaaa
     2821 cagggccgca acggcggcac cgagcaggac aatccaggta aacagcaagc gaccgcgtcc
     2881 attgccccag cgtgaaacgt gcagcgctgc ccattcaaaa aagccggact catccagcag
     2941 caggctgatg ataatgacgg caataaacgc cgccgtcgcg ttccagacga tattccacac
     3001 caccggaata tcacccggat gaaccacgcc cgtaaccaac gccagtaccg cgccgagcgt
     3061 tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacga tggtcaggac
     3121 aaagatagcg cctgccagta acataatgcc tcccggataa aacacatgtg caaatccata
     3181 tgtgtttagc taaattttta actgcaaatg ttcttactgt ctccggaaca gttttgtctg
     3241 gccaggttgc ggacaattgc ctgaatcttt tcctgttcac atcgccaggc ttcatcaata
     3301 attttcgccg cccatgctgg aatatgcggc gataagcggt aatgaaccca cttaccttgc
     3361 ttgcggtcca gcaatagccc gctttcacgc agcaatgcca gatggcggga tatcttgggc
     3421 tgcgactgat cgagggcagt gcagagatcg cagacgcata actctcccag ttcgctaagc
     3481 agtaaaacga tgcccagacg ggtttcatca gcaagaattt tgaacaattg gatgggtaac
     3541 agaaatgaca tattgcgctc ctgattgttg caggtagtgt ctctcttcga agcggataag
     3601 tcaaaaacat atatgactta acgaatgtgt aagtgcagag gaaggtaata ggtgtgaatt
     3661 ttgagttggc tattcatttg aaaggcggtt tgaatcagga cgtaaaaaaa accgactttg
     3721 cgtcggtttt tttactcttc agcctgagtt ggtggctctg gctggagtga gaatgcccac
     3781 atctaacggt tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca
     3841 gctggagaag ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg
     3901 ggtcgctgcc gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg
     3961 tttttttact cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta
     4021 acgcatcaat gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta
     4081 attacactgt atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa
     4141 acgctttcaa agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga
     4201 gtgagattgc ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc
     4261 ggatgcattc tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc
     4321 ttggggcgtt aattgtgctg ctggaactga tccgctttct gcttcagctt ctgaactgat
     4381 agcggaaacg taattaaggg ctaagagcac actactctta gccctttaac atttaacgca
     4441 tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt
     4501 tggttgcgcc catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc
     4561 caatgccgtg aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc
     4621 acggctggcg gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt
     4681 tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa
     4741 tcggcggatg gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct
     4801 tgttattaaa caagcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt
     4861 ccactgcacc cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt
     4921 tatcgacgac gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt
     4981 tgtcattggc aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac
     5041 catcttccag ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg
     5101 cgttggtatg caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca
     5161 tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga
     5221 ggccgttaat cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc
     5281 gctctggcaa agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg
     5341 gaatatccgg gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg
     5401 tttcgccgtt aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat
     5461 caacgttatt tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg
     5521 tacggctggc gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat
     5581 cagggccgta catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg
     5641 gcacgcagcc gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt
     5701 tctggccgta catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg
     5761 cgatgtaatc atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt
     5821 agcgcgagac gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac
     5881 ggtggcgtgc ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt
     5941 catctgctgt tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc
     6001 acggcgatcg ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt
     6061 cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa
     6121 tgcgtaagtg ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg
     6181 atagtcggtt ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg
     6241 cggcagtttg gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc
     6301 tttctgaaat ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag
     6361 gctgtcttgt tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa
     6421 ctggccgcta cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa
     6481 atcgtcctgc tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg
     6541 ttcgctacct aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt
     6601 atctttctct ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc
     6661 gtggttgcca gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag
     6721 taaaaattgg cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag
     6781 gccgccgccg cgaaagcgta acgtttccca ttgaaattca ctacacttaa ccccatgcta
     6841 cacacattat gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct
     6901 aatgacgaat ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga
     6961 acatgtcgtt ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt
     7021 agtagcgcaa ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga
     7081 cgatgttttg gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc
     7141 ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt
     7201 agggcaacat gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc
     7261 cacgctgaac acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc
     7321 tggcataggt ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc
     7381 tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact
     7441 cgttaccgac gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc
     7501 ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct
     7561 gccggtaatg acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag
     7621 cacccgcgcc tccgatcaag gcccgaacgc cggtaagtgg gataacagca aggtgatgga
     7681 agagatcctc gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt
     7741 taaatccctt gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga
     7801 tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc
     7861 taaagccgaa tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa
     7921 acagaaacag cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa
     7981 caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga
     8041 gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa
     8101 taacgaactg cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg
     8161 ggcgtggatg gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc
     8221 ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac
     8281 tcacgacgaa gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac
     8341 ccgcatcgaa accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact
     8401 gccgagtcag tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg
     8461 tcactatgaa accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa
     8521 gaactaccag gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg
     8581 ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat
     8641 caagaaactg gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag
     8701 ccatattttc gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact
     8761 ggcggcagat gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca
     8821 gtcgttcctc gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa
     8881 acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg
     8941 ctgatcattc agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat
     9001 ctgttctggc ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt
     9061 taacgccgga acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg
     9121 ttgattttgt tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg
     9181 cggtggcgaa agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg
     9241 cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg
     9301 agcgtaatcc agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg
     9361 cggaaatcgg cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg
     9421 cgctaaccga tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata
     9481 agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg
     9541 atcttgatgc cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg
     9601 taaagcgccc ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga
     9661 ctaaagggca tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag
     9721 cgataacgct accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg
     9781 cgtttcagcc acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca
     9841 atcttaccaa acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc
     9901 ggcaccgcgg taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg
     9961 aaccacatcc cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc
    10021 aggccaaggg cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata
    10081 aaccatggcg atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagtcgtc
    10141 aaaaagccca gtgagcacat aaacatgccg agagtaaatt tcatcggcat cgagaggtct
    10201 ttgcctttgt tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc
    10261 cagaacgggt taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga
    10321 tgcacgttgt tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa
    10381 aacaccaccg cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta
    10441 tccagcttga atgcctgacg aaagaagatg atggtgacga cgatggagag aacaatcagc
    10501 accagattgg cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg
    10561 ctgccaagta atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa
    10621 ccaatgtctt tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc
    10681 gccccgcaca ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc
    10741 gataacgcta tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca
    10801 agccgcggat ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac
    10861 aggccgttac cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc
    10921 gacatgccgg tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt
    10981 ttggtcccca acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt
    11041 gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg
    11101 aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca
    11161 aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta
    11221 atatttatta accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg
    11281 aaaaagttta catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta
    11341 ttacaacaga aaataaccag ctgatcacac taatgatggt gttaaatttt gtattggcag
    11401 gagattttga ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta
    11461 acaaggcgcg ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga
    11521 gggatgatta ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt
    11581 tgatcagctg acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa
    11641 ccaccagatc catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc
    11701 tgccgctcag ggtttcagtg attggatagc ctgcattagt ggaaagctcg gtcagcgcat
    11761 gatgtgtctc ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg
    11821 tgtataggtc agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc
    11881 gagccataga gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga
    11941 tgagaatgtg tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca
    12001 gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa
    12061 cccagccgta aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg
    12121 tcaatccgcc ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg
    12181 gttaaccttc tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat
    12241 gttggtcacc acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg
    12301 ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag
    12361 caccgtcgca ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt
    12421 ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata
    12481 tgttgatgga gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt
    12541 ttggtatatc tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg
    12601 tgagcgggtg cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag
    12661 cctgattgca ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc
    12721 gggccagtaa actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagaac
    12781 cagtacagcc cgccggaaag tagtaccgca gccggaaggg taaacaccca agccatcaga
    12841 atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca
    12901 gaagaggaga gtacgtgagt ggtggaaacc ggcatcccgg tataactcgc caggccgatt
    12961 gacactgccg ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg
    13021 attttctcac cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac
    13081 gccagcgcga ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg
    13141 gacttcagtt tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact
    13201 ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc
    13261 gacagcttgt cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg
    13321 ttaatggtat tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga
    13381 gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag
    13441 taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc
    13501 atgttcacca cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg
    13561 cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc
    13621 gcgatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg
    13681 gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc
    13741 agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt
    13801 aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt
    13861 aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag
    13921 taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg
    13981 ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag
    14041 gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg
    14101 gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca
    14161 tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc
    14221 ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg
    14281 acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt
    14341 tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg
    14401 caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc
    14461 acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg
    14521 ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc
    14581 tgatcgataa tggtaaaata ctagggcgca aaatccttat gtctggcggt gggcgctgca
    14641 actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta
    14701 agtctgcact cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggta
    14761 tcgcctggca cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg
    14821 tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag
    14881 tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga tctgaacggc atgactgtcg
    14941 gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggtgctg ggcgcatcgc
    15001 cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc
    15061 tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg
    15121 cggtgccttc cgtgattacc actgaaaacg gcaccgtttt ccgtgagaac ttactcttca
    15181 cccaccgcgg cttatctgga ccggcggtgt tgcagatttc aagctactgg caaccgggtg
    15241 aatttgtcag tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc
    15301 gtaacgcaca tccgaatcaa agcctgaaaa acacactggc ggttcatcta ccgaagcggt
    15361 tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg
    15421 tgtgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca
    15481 ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactttctt
    15541 cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg
    15601 ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg
    15661 cgcaggattt ggcgtcaatt gtgaaataac catgacgttt tcccgtcagc cgtttgtata
    15721 tgtcgttatt aagtcatttt ggtacggttc tgcatccgac attctggtgt taactcgctg
    15781 ttttgttagc gaattaacgc tattgccgtt gtggttattt tatagtgt
//
LOCUS       DADLTI010000095        15788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLTI010000095 DADLTI010000000
VERSION     DADLTI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.095"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN755_002662"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN755_002662"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7381414.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN755_002663"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN755_002663"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7381415.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN755_002664"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN755_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7381416.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN755_002665"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN755_002665"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7381417.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="JN755_002666"
     CDS             complement(5464..5661)
                     /locus_tag="JN755_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7381418.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN755_002667"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN755_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7381419.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN755_002668"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN755_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7381420.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN755_002669"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN755_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7381421.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN755_002670"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN755_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7381422.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN755_002671"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN755_002671"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7381423.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN755_002672"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN755_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7381424.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN755_002673"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN755_002673"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7381425.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN755_002674"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN755_002674"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7381426.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..>15788)
                     /gene="nnr"
                     /locus_tag="JN755_002675"
     CDS             complement(14434..>15788)
                     /gene="nnr"
                     /locus_tag="JN755_002675"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7381427.1"
                     /translation="LCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAALAR
                     EAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIVAV
                     DIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWLAG
                     QETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAG
                     LVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQ
                     KVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIESDRLHCAK
                     RLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKLS
                     PYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSAP
                     "
BASE COUNT         3529 a         4559 c         3957 g         3743 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaataaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa
    13141 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagca
//
LOCUS       DADLTI010000096        15703 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLTI010000096 DADLTI010000000
VERSION     DADLTI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.096"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..140)
                     /locus_tag="JN755_002676"
     CDS             complement(<1..140)
                     /locus_tag="JN755_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7381428.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLR"
     gene            complement(134..811)
                     /locus_tag="JN755_002677"
     CDS             complement(134..811)
                     /locus_tag="JN755_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7381429.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(799..1506)
                     /locus_tag="JN755_002678"
                     /pseudo
     CDS             complement(799..1506)
                     /locus_tag="JN755_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            complement(1507..2085)
                     /locus_tag="JN755_002679"
     CDS             complement(1507..2085)
                     /locus_tag="JN755_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7381430.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(2108..2539)
                     /locus_tag="JN755_002680"
     CDS             complement(2108..2539)
                     /locus_tag="JN755_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7381431.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            2911..3930
                     /gene="epd"
                     /locus_tag="JN755_002681"
     CDS             2911..3930
                     /gene="epd"
                     /locus_tag="JN755_002681"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7381432.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            3980..5143
                     /gene="pgk"
                     /locus_tag="JN755_002682"
     CDS             3980..5143
                     /gene="pgk"
                     /locus_tag="JN755_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7381433.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            5358..6437
                     /gene="fbaA"
                     /locus_tag="JN755_002683"
     CDS             5358..6437
                     /gene="fbaA"
                     /locus_tag="JN755_002683"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7381434.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            6628..7488
                     /locus_tag="JN755_002684"
     CDS             6628..7488
                     /locus_tag="JN755_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7381435.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            7627..8262
                     /gene="argO"
                     /locus_tag="JN755_002685"
     CDS             7627..8262
                     /gene="argO"
                     /locus_tag="JN755_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7381436.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            8355..9095
                     /locus_tag="JN755_002686"
     CDS             8355..9095
                     /locus_tag="JN755_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7381437.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            9262..9771
                     /locus_tag="JN755_002687"
     CDS             9262..9771
                     /locus_tag="JN755_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7381438.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            9895..10157
                     /locus_tag="JN755_002688"
                     /pseudo
     CDS             9895..10157
                     /locus_tag="JN755_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(10154..11632)
                     /gene="scpC"
                     /locus_tag="JN755_002689"
     CDS             complement(10154..11632)
                     /gene="scpC"
                     /locus_tag="JN755_002689"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7381439.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(11656..12441)
                     /gene="scpB"
                     /locus_tag="JN755_002690"
     CDS             complement(11656..12441)
                     /gene="scpB"
                     /locus_tag="JN755_002690"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7381440.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(12452..13447)
                     /gene="meaB"
                     /locus_tag="JN755_002691"
     CDS             complement(12452..13447)
                     /gene="meaB"
                     /locus_tag="JN755_002691"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7381441.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(13440..15584)
                     /gene="scpA"
                     /locus_tag="JN755_002692"
     CDS             complement(13440..15584)
                     /gene="scpA"
                     /locus_tag="JN755_002692"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY7381442.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
BASE COUNT         3799 a         3982 c         4123 g         3799 t
ORIGIN      
        1 cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca ttcaccttgt
       61 ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca gcgataagat
      121 atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc cacgcttgct
      181 ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc gaaccacgtt
      241 caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggc gtcaggcggc
      301 taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag gcgatgacca
      361 cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac ggctggcact
      421 cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca agacacagat
      481 tttctggccc aaaggcaact tcttcttcca cgctacaggt acagccggag agctgcaaat
      541 agggcgattg ctgaacgagc tggatggtgg cggattgttc attaagcggg agatgcccaa
      601 tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag tccggatacc
      661 agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca aagatggcga
      721 ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt gagtgtgtcg
      781 ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg tcagcatcag
      841 caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg cccacaacgt
      901 tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac ggacagccag
      961 ctcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac gtaaacgctg
     1021 ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa taatcgctaa
     1081 ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag cgacgcctgg
     1141 cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt actgcattca
     1201 caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag catgcgacca
     1261 ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac cgtgtaccag
     1321 ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc gccgccgcgt
     1381 ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca aaatggtctg
     1441 cgcgggcagg ataagcgtgg tgcaggccgc taatgcccac agtgttaatg aggtaaatgg
     1501 atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa tcggcagctg
     1561 gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt tcgcgcctat
     1621 caccgtaatc gccaccgact ccaccaagtt ttgccccatc gagtgcatcc aggcaacgaa
     1681 caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg gtacagcgac
     1741 gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca gggtgcgaaa
     1801 ccaacccgct cgcgccagcc aaccggagac caggccgatg accatcgcga cgggtgcaaa
     1861 tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta agccggtcag
     1921 cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg agtcgagaaa
     1981 tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg cgatagatat
     2041 aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa aacctctttg
     2101 aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc gcgcaaggtt
     2161 ggcggctaaa ctccagttcc tcaaggctac cgatcaccag ttcaagcgtg gtccgcacgg
     2221 tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa acgcagagat
     2281 gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg aatgtaccgt
     2341 tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc aggctaccgg
     2401 tacagcctgc aatccaggcg gcatgaagtt gatgttgctg tgcaaaagcg tgtagctggg
     2461 aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag gaagaatatg
     2521 ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg ataatatcga
     2581 cgctgcatca gccatattct gatataagtc agggtttgtg acggcacaac gaaaagattt
     2641 tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg gggctgaaaa
     2701 cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt tattccacgt
     2761 ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta accaatcagt
     2821 cgcagtttgc gacaggtaag gtttccccgg acgatttgct agattactct gtctggcaaa
     2881 tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat aaatggcttc
     2941 ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg ggcggaaatt
     3001 accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt gttgaaatat
     3061 gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca actttttgtt
     3121 ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc ctggcgtgaa
     3181 cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga gcatagcgaa
     3241 gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag taacgatctc
     3301 ggcgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca ccgcatcgtt
     3361 tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt agatgatgcg
     3421 tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga tcaacaggtt
     3481 attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc gatcattccg
     3541 gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa cgatcgcttt
     3601 gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt aagcgtgacg
     3661 gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc agcacaaggt
     3721 gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga ttttaaccac
     3781 gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc acacctgatc
     3841 aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct cgacacgacg
     3901 ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa aacttttaga
     3961 atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc ttgctgggaa
     4021 acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag taaccagcga
     4081 cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag gcgcaaaagt
     4141 gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag aattctctct
     4201 gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc tggttaaaga
     4261 ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa acgttcgctt
     4321 caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg cactgtgtga
     4381 cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta ctcacggtat
     4441 cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac tggacgcgct
     4501 gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg gttctaaagt
     4561 atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc tgattgttgg
     4621 tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta aatccctgta
     4681 cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca tcccggttcc
     4741 gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc tgaaatctgt
     4801 taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg ctcaggaact
     4861 ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg gcgtgttcga
     4921 attcccgaac ttccgcaaag gtactgaaat cgtggctaac gctatcgcag acagcgaagc
     4981 gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg gcattgctga
     5041 caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg aaggtaaagt
     5101 actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca cagggcaggg
     5161 aaacctgccc ttgtttcagc gcgcttttag agcacgcatt ttttcatact ctaaataatt
     5221 cgagttgcag gaaggcgaca agagagtgaa tccccaggag cttacataag taagtgactg
     5281 gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat aaggcccgac
     5341 gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc gtaatcactg
     5401 gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca ctgccagcag
     5461 taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct aaagttaaag
     5521 cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt aaaggcgtga
     5581 aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg catcacgttc
     5641 accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac tgcgcgaaga
     5701 aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc gcagctaccg
     5761 gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg caagagaaca
     5821 tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact ctggaaatcg
     5881 aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg gacgcttctg
     5941 cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc aaaatcagcc
     6001 cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag ccgggtaacg
     6061 tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag aaacacaacc
     6121 tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact gctcaggaaa
     6181 tcaaagactc cgtaagctac ggcgtagtga aaatgaacat cgataccgat acccaatggg
     6241 caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg cagggtcagc
     6301 tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg cgcgtatggc
     6361 tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag gaactgaacg
     6421 cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc tgcgggtctt
     6481 tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg gcaaaattat
     6541 gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt atttctccat
     6601 tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga tagcataaac
     6661 ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc agtaaacatc
     6721 gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat ttccaacgcg
     6781 gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt tctttctgca
     6841 ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg cgtgggtgta
     6901 caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg tctggctttg
     6961 caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg cccgttccgt
     7021 gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt gcagattttc
     7081 tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg taaaattatt
     7141 gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt tattattggc
     7201 gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat tatccagtct
     7261 gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact tggtgcatcg
     7321 tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa cgtgtactgg
     7381 gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt cccgtacccg
     7441 caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc aacgctgagg
     7501 ccagataata ctctctggcc tctctcttat tagttattct gattgccaat taatattatc
     7561 aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta attcggaata
     7621 tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat gattctgccg
     7681 ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta ccacattatg
     7741 attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat ttttggtggt
     7801 agcgcgttat tgatgcagtc gccgtggttg ctagcgctgg tcacttgggg cggcgtagtc
     7861 ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa tattgagtta
     7921 gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat gttggcagtg
     7981 acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag ccttggcggg
     8041 cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc ctctttcctg
     8101 tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa
     8161 tcacagcaca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc cttgcagctg
     8221 gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc tgatggaaaa
     8281 ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa gtcaattaac
     8341 acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg ggtattagcg
     8401 ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc tccggtacgg
     8461 caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac gtggccgcga
     8521 aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac atttccttcc
     8581 ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc acccagccag
     8641 attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg agaacggtgg
     8701 aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg ctgaaggcgg
     8761 gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc tataaagaca
     8821 aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg gcgaacggct
     8881 ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac tatcagccca
     8941 gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag gaaacttacg
     9001 agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta gaacctgtgg
     9061 atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac gtattcgtcg
     9121 ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa aaccatgacg
     9181 taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac ggttaagcgg
     9241 acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg caatttcatt
     9301 ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat gacggcttcg
     9361 ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct atttacccgc
     9421 aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa actgttcccg
     9481 gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg tcgttcacga
     9541 gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca gttgattagt
     9601 ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga aaacggcgtt
     9661 atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca tgtatctgca
     9721 cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgtg attgggcttt
     9781 ctcgtttctc gccgttataa agagcgtcaa gagcaagagc tgcttcagga gctcccctgg
     9841 ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga
     9901 ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc
     9961 gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggtg actggtgaat
    10021 ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca ggaatatctt
    10081 tattcaatca gaaatcatcg ttataccgcc acgatcttca gcatattgct gaagatcgtg
    10141 acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg tgtaagtcga
    10201 agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc attttccaga tagcgatgca
    10261 gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg
    10321 gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg acgctgtgtt
    10381 cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct tctttagcaa
    10441 tcgacggagc cataaagatc gacagatacg cgttgcgttc aaaatcaccg ctgccgccga
    10501 tgccgttcat cagatcgacc ccggctacgt gcgttgaatt tgcatgcccg taaatatcaa
    10561 actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc gggttattgg
    10621 aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg tcgtaaatct
    10681 tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg attttgccgg
    10741 tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcatg aacggaggaa
    10801 tttccgggtt ttcccccaga cgcgccatta ccgcattatt gatattgccc acgccacttt
    10861 gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat aagaacgtga
    10921 ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata ttaccggcgt
    10981 cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc acatagcggg
    11041 taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt ggcgcgccag
    11101 gaatcacaat atctgccagt tctgcaacgc gcggatcgtg atagtgatta agttcaatga
    11161 tcactttctt cgctcgcagc agccaggtcg gcgcattacc gatcccgctg gttaaccaga
    11221 ctcgaccatc cggtgccagt gccgatgctt caatgacggc aacatcaatg tcgccgaaga
    11281 aaccgtaatt gaccatttgc gccacttcgc tcaaatgtag gtcaacgaaa ctcaccgcgc
    11341 cctgattgat ctttttacgt aaactggacg atgtttgata tggcgcacgc caggaaacag
    11401 catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc
    11461 gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggcgg
    11521 gtagggcttt cggcgaaccc gctggggtaa agccgctaaa tgccaccatg tcgttatgct
    11581 ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc
    11641 cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt
    11701 catcccttcc tggtaatctt cgctgtcata aaccgcgcgg cgcatcccct gaatacgttc
    11761 aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca cgcagctctt ctttgataac
    11821 ggcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc
    11881 cagttcttcc acttccacaa catggttgag gatgccgaca gccagcgcgc gctgggcggt
    11941 gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag
    12001 gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt
    12061 tgaggtactg gcggcgatga tcagatcgga actcatgatc atttcaaatg cgccacccca
    12121 aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca tgcgggtgat
    12181 ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat
    12241 atcgtgacct gcggagaaga ctttggatcc acttggcgcc cgcaaaatga tacagcgaat
    12301 ttccggccgg ttgagatcgc ttaacgcctg cataagatca tcaataaaga ctttacttaa
    12361 ggcattaagt tttcgtccat agttaaactc aatgaccgcc actttgttga tagtggcaac
    12421 gttcacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt
    12481 cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg
    12541 tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt
    12601 cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac
    12661 tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg attccacgtt
    12721 tttccagtgc gctacaagtc agaccccgtg gctgccattc gtcgtattta cgtcgcagaa
    12781 tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat
    12841 cgtctttatt gataacgagc agatcagcca cttccatcag ccctttttta atgccctgca
    12901 gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt
    12961 ccgtttccga ctgcgcgacg ccaaccgttt cgacaatcac tacgtcataa cccgccgctt
    13021 cgcataacag cattaattcc cgtgctcgct gactggcacc gcccagatga ccggaggagg
    13081 gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc attcatgcgg gttttatccc
    13141 cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataaca gcaaccttta
    13201 atccctcacg aatcaacaac atgccaaagg cttcaagaaa ggtacttttc cccgcgccgg
    13261 gtgtgccggt aacgcccagt cgcagggcgt taccacagaa cggcataata gcgtcaagca
    13321 gctgtgtact tagcgcctga tgacgtgggt gacggctttc caccagcgtc atggcttggg
    13381 cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat
    13441 taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta
    13501 cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggcgga
    13561 atgacgccac ccgcgaccac gcagatatct tcgcgtcccc attttttcag cgcttcgacc
    13621 agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccgc tacgtgaacg
    13681 tcgttttcta cggccaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct
    13741 acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca
    13801 tcctggccca tcttagcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc
    13861 tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagactg atgatagctt
    13921 tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa agcgacttcc
    13981 agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc
    14041 agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg
    14101 gcatcatcac gggcggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatt
    14161 acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca
    14221 ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg
    14281 gtcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc
    14341 tgttggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag
    14401 gatccggcca gtggatcgac ggtgtggcag agttctgatt cttcctggat gatgatctgg
    14461 gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg
    14521 ttggtatgca gtgactgagt accgcccagc gtcgcagcca gtgcttcaat ggtggtgcgg
    14581 ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta
    14641 cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac tgaccgcttc gctccataaa
    14701 taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat accgaagaat
    14761 aacgacaggc gaggagcgaa gtcatccatt ttcagtccgg cagagattgc tgctttgatg
    14821 tactcaatcc catcggcgag cgtaaatgct acctgctgca cgcagttggc acccgcttcc
    14881 cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag
    14941 gcgatgatgt cggcgataat gcgcattgac ggttttggtg ggtaaatata ggtgttgcgg
    15001 cagaggtact ctttgagaat atcgttttga atggtgccgg tcagtttatc aggtgtaaca
    15061 ccttgctctt ctgcggcgac gatataaaac gccagtactg gtagcactgc gccattcatg
    15121 gtcatcgaaa ccgacatttt acccagcggg atctggtcga acaggacttt catatcttcc
    15181 acggtgtcga tagcgacgcc cgctttgccg acgtcacccg ccacgcgtgg gttatcggag
    15241 tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc
    15301 aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg
    15361 atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa
    15421 ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg
    15481 atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc
    15541 aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg ctccgtaaaa
    15601 cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag
    15661 caaaaaaaat gcattcagac gtcgtttttt gccgcctgag att
//
LOCUS       DADLTI010000097        15600 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLTI010000097 DADLTI010000000
VERSION     DADLTI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.097"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..587
                     /locus_tag="JN755_002693"
     CDS             <1..587
                     /locus_tag="JN755_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7381443.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            584..2035
                     /gene="djlC"
                     /locus_tag="JN755_002694"
     CDS             584..2035
                     /gene="djlC"
                     /locus_tag="JN755_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7381444.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2095..3765)
                     /locus_tag="JN755_002695"
     CDS             complement(2095..3765)
                     /locus_tag="JN755_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7381445.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="JN755_002696"
     CDS             complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="JN755_002696"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7381446.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="JN755_002697"
     CDS             complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="JN755_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7381447.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="JN755_002698"
     CDS             complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="JN755_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7381448.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="JN755_002699"
                     /pseudo
     CDS             complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="JN755_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="JN755_002700"
                     /pseudo
     CDS             complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="JN755_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="JN755_002701"
     CDS             complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="JN755_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7381449.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10078..10956)
                     /gene="corC"
                     /locus_tag="JN755_002702"
     CDS             complement(10078..10956)
                     /gene="corC"
                     /locus_tag="JN755_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7381450.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="JN755_002703"
     CDS             complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="JN755_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7381451.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11510..12589)
                     /locus_tag="JN755_002704"
     CDS             complement(11510..12589)
                     /locus_tag="JN755_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7381452.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="JN755_002705"
     CDS             complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="JN755_002705"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7381453.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14273..15448
                     /gene="ubiF"
                     /locus_tag="JN755_002706"
     CDS             14273..15448
                     /gene="ubiF"
                     /locus_tag="JN755_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7381454.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3777 a         3992 c         4059 g         3772 t
ORIGIN      
        1 tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga
       61 ctgatgatgg tcatgccaat gatttagctg gcttatatct gacatggcaa cgctcttccc
      121 ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat
      181 tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcctgg gattatgttc
      241 gaatggggtt tctgtcacgg gccggtgtgc tcaacaactg gctgacggaa gaagagagtt
      301 tatggctgca atcgcgcgtc tatgcacgag ctcaccatta ctatcacagc tggatgcact
      361 atttttctgc ctattcacta gggcgtctct actggcaatc ttctcagtgc gaagacaacg
      421 cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg
      481 aagagctggc agcgggaagc gaccgtttct atgccacgct tccctggcag ccattaactg
      541 tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca
      601 aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg
      661 cttaccgttg tgtcatcccg aaaccgatcc gcaggggttt aaagcattac gccaggccta
      721 tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga
      781 tgccaccgct gaacatgaaa tactacgtgc attcaggaca ttactggatt cagaaagtga
      841 tcgttttcag ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga
      901 ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctgtc
      961 attaaattgt gcttctttgt tggcagagcg tttgaactgg cattcgttta atgacagcga
     1021 aggaatggat gaggaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt
     1081 cgatttcctt agccttctgg aatatcccgt cgcgttacag aaccagactg ttgaatatta
     1141 cttcgcgctg gaacgttgct gccgttacca tcctgactat gtcactgcgt ttttggcgat
     1201 ggaaggaccg tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta
     1261 cagctcggtg caaacaggta tggtggaact cattcctgta gccaaacagt ggcaagcgga
     1321 agaaccagaa agcgaagatg cccggtatta ccagtgtgca caacgtttgt actgcggcga
     1381 gggggaaagc ctgcttgctg atctcggcgc gtactgggaa agttacccat ctacacaagc
     1441 tgataatttg ttgttgcagt ggagcaagcg tcattgcccg gattatttcg cgttattagt
     1501 gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt caaccgctga aatatgttcc
     1561 tggtgagagc gcccggacgc ggctgttatg ggcggaaatt ttacatagcg gaaaattatc
     1621 gccgttaggt caatcgttta ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg
     1681 gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt
     1741 agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac
     1801 aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac
     1861 gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga
     1921 agaaaaaaat gatgagggtg aaaaaccaca gagcattacc agcattatca aaatcagttt
     1981 aacggtgctg gttataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg
     2041 tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatga
     2101 ggtctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc
     2161 gtttgactgc agcgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc
     2221 acccagcgcc cgcgcccaca actcttccag tttggcttta aaggtgcgat tgatgagcat
     2281 atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc
     2341 gctcaatgtt accgggctgt ggttaatcac tctggactta acgctgccgt cttccagaag
     2401 cacgtcaact tcaagcaacc cgttgatatc ataactaaag cgaatatcaa tcgactgata
     2461 agccccggtt ttcttcaacg gcacatcgaa ggattccacc agaatgttgt ttttaacttt
     2521 gtggttttct ccctgataga cgttaaccgt aattgaatcc tgttccgggt gcatggttga
     2581 ataagtttct acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg gcgagaaaat
     2641 gccggaaacg ccctggcggt taacctcaac gcccaacgag taagggcaaa tatcagtgag
     2701 gatcacttct tcaatatctt cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag
     2761 cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc
     2821 gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg
     2881 actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag
     2941 cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc
     3001 ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg cttccacaca
     3061 ggcgtacaag gctgccagtt cactctcgtt aagcgtggtc cttgtgacat ccgcgcgctt
     3121 taaaacgtca tcgaccaaca tatgggtaaa atcttcgcca ccgaggaagt tgtcgccagc
     3181 ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc
     3241 gccaccgaga tcaaacacca gcgaacgggt attttgttgg gtatgcaggc catacgccat
     3301 cgcagctgct gtgggttcat taattaagcg taccgcattt aacccggcta agtccgctgc
     3361 taaacgggta tgcttgcgtt gtttatcgct gaaataagcc ggaacggaga tcaccacatc
     3421 tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga
     3481 ggacagttct ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat
     3541 tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg
     3601 ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc
     3661 accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt
     3721 ggtagtgccg agatcgatac ctatggcgag ttctgcatta tccattgcat ttatatcttg
     3781 aaagaaaaag agataaatag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3841 gacgagtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     3901 tcaacatcga caattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     3961 accgtcatac ccagggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4021 gtaaacaact cgggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcgccg
     4081 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4141 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgc
     4201 gctttatgag taacatccag accggccatc accaccggga tccctgactg gaagacaatt
     4261 tctgccgctt ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttaccaagc
     4321 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4381 ttgagcagca aggcaacgtt agtttgcggt ccggtagaaa caatcgtgac aggttccgca
     4441 ctttcgcgca gcgttttcgc catcagctcc accgccgtac agttttgcgg tgcgaacgtc
     4501 ggttccggta atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc
     4561 aactcacgca ttaacggttt taccgcgccg cctgccaccg gaatatcggt gcgattaagc
     4621 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4681 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4741 tcatcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4801 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4861 agcgtgagat ggcgcgcaat tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     4921 aggaagtctt ttgcgcggtc cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct
     4981 tcgacaattt taccctcgtc cataaagatc acccgattcg ccactttgcg ggcaaagccc
     5041 atttcgtggg tcaccaccat catggtcatt ccttcgttcg ccagttccac catcacgtcc
     5101 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca
     5161 ataggagcca tacacaatgc gcgagcgatt gccacacgct gctgctgacc gccggaaagc
     5221 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5281 ttttcacgag cgggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5341 tcgataatcg acagatgagg gaacagctcg aaatgctgga ataccatccc gacgcgggag
     5401 cgcagctttg ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca
     5461 ccttgctgca ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5521 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5581 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5641 ttttcaagta gctgaccaac aacgacgcgc taaggctaat aacgaaataa acaaatccgg
     5701 caaacaggat catctcgacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5761 agaaatcggc cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct
     5821 gagtgagcag cagcggcacc atcgcgcgga aagcctgcgg cagaataatc agtttcatcg
     5881 actgccagtg agtcatcccc aacgccagcg cggcgctcga ctgaccacgg gaaatacttt
     5941 gaataccggc acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca
     6001 tcgccgaaat taaacgaata tcattttttg gcgataatcc cagcacattt tgcagaaatc
     6061 ccggcacaat caagtaaaac cacagcaaaa ccatgactaa aggaatcgag cggaatacgt
     6121 taacgtaggc tttggcaaac cacgaaacag gcgcgaagct ggacaagcgc atcaccgcca
     6181 gcatggtacc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agggtgatca
     6241 ccagcccgtc gagcagatat ggcagggaag gaacaatgga actccagtca aactcgtaca
     6301 ttatttgccc cccatgttgc cgggcaggcg aactttacgt tcaaccagcg tcatcaccag
     6361 catgataaaa gcgttaatca acacatacgt aagcgtgatg gcggtaaacg actcccaggc
     6421 gtgagccgag tagtcgagca atttacccgc ctgcgccgcc atatccacca gaccgatggt
     6481 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6541 aacgcgataa gcattaggca gcagtacgta tcgataagtc tgcggtagcg tcaggcccat
     6601 cgccagcgcg gcattcttct gcccacgcgg tagcgactga atagcggcgc gtacctgttc
     6661 gcaaacacgg gaggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6721 attgggatcc agctcggcct taaaccacat gccgattttc tccggcagca gctccgggat
     6781 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6841 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     6901 accgacgagg aaagcgataa ttcaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     6961 actccagacc caaccaagat aggtggtatt gccgaacggg gcttgttgta aaaaaatacc
     7021 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7081 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7141 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcctctg
     7201 gttcttgtaa ttagttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7261 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat tggatttttg aaccatttat
     7321 caaaccattt ttccgcttca ccggaggtct gcacctgagc gatagtgtca tccatcagct
     7381 ttttgaactg cggatcgtct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7441 tgccgacgat atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcagag
     7501 cgtcatccat cataaaggca acggcacgac cgctttccag ggtgcggaaa gagtcaccgt
     7561 gatctttggc gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7621 aaacttcaga ggtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat
     7681 ctttgatatc gccgcccttt ttggtcaaca ggcgcgtacc gaccacgaaa atagtgtcag
     7741 agaaagccgc ctatttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7801 aagtgccgtt ttgcagcagt ggaatacggt ttgtgaggta atcggaatca gttttacctg
     7861 caagtccggt ttgttgagtt tctttttcac tgcttcaaca atggcgttgg agtaatcctg
     7921 cgagtaaccc accacttttt gctgattgtc gtaataagag aaaggtactg aagattcacg
     7981 gtgaccgacg acaatcacac cgtttttggc gattttgtcc agcgtactgc ctgctgccgg
     8041 ggcggcgtca tctgcctgtg ccagtcctgc ggaaagcgcc agggcgagga ttgctgtggc
     8101 aggtttacgt aattgcatat ccaactcctt tatcttctgc gttaagaacg catggatacc
     8161 cgttgtgagt gtttgtgttg ttatcgtctg caactttatt gtgcagtgtt gtgcctgtta
     8221 ggtaacattt agtttggcta aatgtaaaga tattgatgtt ttattgtttg tttttgcgag
     8281 gtgcaccgca ccattctgaa gcaaaattct taaaatgcac tcttttggtg ctaccggtgg
     8341 attgctgtgg tgcaaccggg ttgtactgat gctggttcaa gctttgcctt gtataacaaa
     8401 gcaatagatg tgccaaagtt ggataggaga atattgttat ccggataatg cactgatgcc
     8461 gcatccggtg agcgtgccga aatatgggat gtattccggc acgataagaa gggattattt
     8521 acgtcgctga cgcagactca tcaacacagc agcaaaacca aacagtgccg tcagcaccca
     8581 cagcggccag ttgccggtac gtgcgtatgg tgtgagtccg gtggtcggcg tcacgttagt
     8641 ggttaacacc tcgcgggtga actgcgggat catcgcctga atctcaccct gcgggccaat
     8701 caccgccgta atgccgttgt tggtgctgcg caacagtggg cgcgccagct ccagcgcacg
     8761 cattcgcgcc atctggaagt gttgccatgg accaatagat ttaccaaacc acgcatcgtt
     8821 ggagatagtc agcaggtagt cggtatccgg gcggaagtta tcgcgcactt gctcgccgag
     8881 aatgatctcg tagcaaatag ccgcagtaag ctcaatacca tttgccgaca gcggcggctg
     8941 gatatatggc ccacggctga acgacgacat cggcagatca aagaacggtg ctaacggacg
     9001 cagaatcgac tccagcggga caaactcgcc aaacggcacc agatggtttt tgttatagcg
     9061 atcggctgat tcgtagctgt acggtgcacc tttacccagc gtgatgatgg tgttgtaggt
     9121 atcgtagcgg ttctgcttat tgagacgcgc gtcgacaatc ccggttacca gcgagctacc
     9181 tttatcacgc aactcaccgt ccagtgcttt gaggaacggt tgctgattaa tttccagatc
     9241 ggttatcgcc gactccggcc agataatcaa cgatgatttg cccatcagcg gtgccgttgc
     9301 gttgtagtaa atctttagcg tattaagaag ctggccttcg tcccatttca gcgattgcgg
     9361 aatatcgccc tgaaccatcg aaacctgaat ggttttctcc ggttgcgggg taaaccattg
     9421 gatgtaacgc agcgggaagg gtagggcaaa cagcacgacg gccaccacca gcggacgcca
     9481 gttgcgtttg accaacgcca gtgccagcag gccactaacc atcatcagca ggaagttaat
     9541 ggcttccacg cccattagcg gtgccagccc ttttaacggg ccatcaatct ggctatagcc
     9601 gaactgtaac cacgggaagc cagtcagcac ccaaccgcgc agaaactcgg tcacttgcca
     9661 gagagcaggg gcggcaatcg ccacgcgcag ccaggtggtt ttcggccaca gacgcgacag
     9721 cacgccagca aacagtccgg tatacagcga caaatacgcc gccagcagca ccactaggaa
     9781 gatgttaacc gggccaggca tcccgccaaa ggtcgcgatg ctgacataga cccagttaat
     9841 accgctgcca aagaggccaa atccccagca aaagccaata gcggcagact ggagtgggcg
     9901 acggttaaag gtcaacgcct gaagccccat cagcgaaata atcgccgcag gccagacgtc
     9961 gtaaggagag aaggccagcg ttccgcaggc accgaataat aacgccagca gcaggcgaat
    10021 gcgctggcgt tcaattaatg aggcaaaagc catgtagtta tctatccagt ttcggtttta
    10081 ttcatccagc tttggctggg gtgagtcatc cgggattttg acatgaacct gaataatacg
    10141 ccgactatcg gccatcgcca ctttgaactg gtaaccgtcg atgtcgatag tttcgccacg
    10201 cgccggaaga tgcccaaacg cctgcatcac cagaccaccg atagtgtcga cttcttcatc
    10261 gctaaagtgg gtgccgaacg cttcgttgaa gtcttcaatg gaagccagtg cgcgcacggt
    10321 ccaggtatga cgactcagct gacggaagtc gatatcatct tcttcgtcat actcgtcttc
    10381 aatctcacca acaatcagtt ccaggatgtc ttcaatggtc accagaccgg aaaccccacc
    10441 gaattcgtca ataacgatcg ccatgtggta acgctgagag cgaaactctt tcagcatccg
    10501 gtctacgcgc ttactttcag gaacgacaac cgcctgacgt aacactttgt ccatgccgaa
    10561 ggcttcagca tcgctgcgca taaacggcag caagtctttc gccatcagaa tcccttcaat
    10621 gtgatctttg tcttcgctaa tcaccgggaa acgtgagtgg gcggactcga tgatgacatc
    10681 aaggcattcg tccagcgtct ggttgcgttt cagggtaatc atctgggagc gggggatcat
    10741 gatgtcgcga acgcgttggt ctgcgatgtc catcacccct tcgagcatat cgcgcgtatc
    10801 ttcgtcgata aggtcgttct gcccggaatc acggatcagc gccagcagtt cgtcacggtt
    10861 tttcggttca ccgtggaaaa gttggctgag taacagggag aaaaatccct tcttgttgct
    10921 tatcgtgtca ctactgtgtg aattgtcgtc gctcatggcg tcgtatgggt tctcatgtta
    10981 gttaatcaaa acgccgtcgt taatcaccaa cggcggggac gtctgccagt caaatgcctg
    11041 gcaaattatt ctttctcggc aatgtacgga tcctcatagc ccagagcaag cataatctct
    11101 gtttcgaggg cttccatttc ttctgcttcg tcatcttcaa tgtgatcgta acctaacaaa
    11161 tgcagactgc cgtgcaccac catatgcgcc cagtgcgcct ccagtggttt gccttgctcc
    11221 tgagcttcct tctcaaccac ctgacggcag ataaccagat cgcccagtag cgacatctcc
    11281 atgccaggcg gcacttcaaa cgggaaggag agcacgttgg tcggcttatc cttaccgcgg
    11341 taggtcagat tcagactgtg gctttcggcg gtatcgacca cgcgaatagt cacttccgac
    11401 tcttcctgaa actgcgggat caccgcattc agccatgttt gaaactggct ctcttccggt
    11461 aacccggaat tatcttcaca tgccagttgt aaatcgagga tcacctgact catttttgtt
    11521 cctgttcttc gcgcttgcgt tctgctgcca gcgccgcttt acgtttttgt tctgcttctt
    11581 ccctggcttc ataagcgtta acgatacgcg ccaccaccgg gtgacgaacc acgtcttcgc
    11641 tgtggaagaa gttaaagctg atctcttcga catcggccag cacttcaatg gcatgacgta
    11701 agcctgattt agtattacgc ggcaggtcga tctgtgtgac gtcgccggtg ataaccgctt
    11761 ttgagttaaa accgatacgg gtcaggaaca tcttcatctg ttcgatggta gtgttctggc
    11821 tctcatcgag aatgataaac gcgtcgttca gcgtacgacc acgcatatag gccagcggcg
    11881 cgacttcaat aacgttgcgc tcaatcagtt tctcgacttt ctcaaagccc agcatttcaa
    11941 acagcgcgtc gtacagtggg cgcagatacg ggtctacttt ctggcttaaa tcgccaggca
    12001 ggaagcccag tttctcaccg gcttcgaccg ccggacgagt cagcagaata cggcgaattt
    12061 cctgacgctc cagggcatca actgccgcag ccactgccag gtaggtttta cccgtacccg
    12121 ccgggccaac gccgaaggta atgtcatggt cgagaatatt ggcgatgtac tgcgcctggt
    12181 ttggcgtgcg cggcttaatt acgccgcgtt tagttttgat attgaccgct ttgccgtact
    12241 ccggcacgct ctccgcgctt tgctccagta cccgcgcttc tttaatcgca aggtggatct
    12301 gttccggttc gatatcctga atctgaccgc gcatcggggc agtatcgaca tacaggctac
    12361 gcagaatgtc tgccgcagcg gtgacgcaaa tcggacggcc ggtcagttta aagtggttat
    12421 cgcggcgatt gatctcgatg ccgagacggc gttcgagctg cttgatgttg tcatcaaacg
    12481 ggccgcacag gctcaacaga cgcgcattgt ctgctggctc cagggtgatt tcgcgagtgt
    12541 ctatgttcaa accgttcctc ttatctgtat gccgccggaa gctgaacatt caccggccta
    12601 taaggaaatt attcacgcca caggaaaaag gcgcaagcat tgcgataaag atggggataa
    12661 agagagaaaa aacaaggccc accggaacgg caggcctgag aattacggct gataataacc
    12721 cacgccaagg tcgttttctt tgcgggtgcg ggcaatcacc gactccggtg tttctgccat
    12781 gcgcagaccc atttcatctt cagttcgcac cactttaccg cgcagagagt tcgggtagac
    12841 gtcggtaatt tctacatcga cgaatttacc gatcatatcc ggtgtgccct cgaagttgac
    12901 cacgcggtta ttttcggtac gcccggaaag ctccatgatg ctcttacgcg aagtcccttc
    12961 taccagaata cgctgggtgg tgccgagcat ccgacggctc catgccatcg cttgctgatt
    13021 gatacgttct tgcagaatat acagacgctg cttcttctct tcttccggaa catcatcaac
    13081 catatcggcg gcaggcgttc ctggacgtgc agagaagata aagctgtagc tcatgtcgaa
    13141 attgacgtcg gcaatcagct tcatcgtttt ctcgaagtct tcggtggttt cgccagggaa
    13201 gccaacaatg aagtcagaac tgatctgaat atccggacgc gccgcacgca gtttacggat
    13261 gatcgctttg tactccagcg ccgtatgggt acgtcccatc aggttcagaa tgcgatcgga
    13321 accgctctgt accggcagat gcaggaagct caccagctca ggcgtgtcgc ggtacacttc
    13381 gatgatatcg tcggtgaatt cgatcggatg gctggtggta aagcgaatac gatcgatccc
    13441 gtcgatcgca gcaaccagac gcaacagatc ggcaaacgat ccggtggtgc cgtcgtagtt
    13501 ctcaccacgc caggcattca cattctgacc gagcaggttg acttcacgca cgccctgagc
    13561 cgcaagctgg gcaatctcaa acagaatatc gtcagacgga cggcttacct cttcaccacg
    13621 ggtgtaaggc accacgcagt aggtgcaata tttattgcag ccttccatga tggagacaaa
    13681 cgcggtcggc ccttcggcgc gcggttccgg cagacggtca aacttctcga tttccgggaa
    13741 gctgatatct acaaccgggc tgcggtcgcc gcgcacggag ttgatcatct ccggcagacg
    13801 gtgcagcgtt tgcggcccaa aaataatatc gacatagtgg gcgcgctggc gaatgtgctc
    13861 gccttcttgc gatgccacgc agccaccgac gccgataatc aggtctggat tcttctcttt
    13921 taacagtttc cagcgaccca actgatggaa gactttttcc tgagccttct cgcggattga
    13981 gcaggtgttc agcagtagca catccgcttc ttccgccacg tcggtcagtt gatagccgtg
    14041 ggtggcatcc agcagatcgg ccatcttcga tgaatcgtac tcgttcatct gacagcccca
    14101 ggttttaata tggagttttt tggtcatcga cttgctcttg cgaaatagta gccaggaatg
    14161 cagggcgcat agtgtaatgc tttgctgccg ttgtgaccag tatgagcgtt atcagccctt
    14221 gggggtaaaa atcctgtaaa cttaaagcat attgctaaca ggatgattga ccatgacaaa
    14281 tcaaccaacg gaaattgcca ttgtcggcgg aggaatggtc ggcggcgcac tggcgctggg
    14341 gctggcacag cacggatttt cggtaacggt gatcgagcac gcagaacctg caccgtttgt
    14401 cgctgacagc caaccggacg tgcggatctc ggcgatcagc gcggcttcgg tatcattgct
    14461 taaaggggta ggggtctggg atgcagtaca ggctatgcgt tgccatcctt accgcagact
    14521 ggaaacctgg gagtgggaaa cggcacatgt ggtatttgac gcagccgaat taaagttgcc
    14581 gttgcttggc tacatggtgg aaaacacagt cctgcaacag gcgttgtggc aggcgctgga
    14641 agcgcatccg aaagtaacgt tacgtgtgcc aacctcgctg attgcattac atcgagataa
    14701 tgatcttcag gagctggaac tgaagggcgg tgaaacgatc cgcgcgaaac tggtgattgg
    14761 tgccgacggc gcaaattctc aggtgcggca gatggcggga attggcgttc atgcctggca
    14821 gtatgcgcag tcgtgcatgt tgattagcgt acagtgtgag aacgatcccg gcgacagcac
    14881 ctggcagcaa tttaccccgg acggaccgcg tgcgtttctg ccgttgtttg ataactgggc
    14941 atcgctggtg tggtatgact ctccggcgcg tattcgccag ttgcagaata tgaatatggc
    15001 gcaactccag gcggaaatcg cgaagcattt cccgtcgcgt ctgggttacg ttacaccgct
    15061 tgccgctggt gcgtttccgc tgacgcgccg acatgcgttg cagtatgtgc agccggggct
    15121 tgcgctggtg ggcgatgccg cgcataccat ccatccgctg gcggggcagg gggtgaatct
    15181 tggttatcgt gatgtcgatg ccctgattga tgttctggtg aatgcccgca gctacggtga
    15241 agcgtgggcc agttatcctg tcctcaagcg ttaccagatg cggcgcatgg cggataactt
    15301 cattatgcaa agtggtatgg atctgtttta tgccggattc agtaataatc tgccgccgct
    15361 gcgttttgtg cgtaatctcg ggttaatggc ggcagagcgt gctggcgtgt tgaaacgtca
    15421 ggcgctgaaa tatgcgttag ggttgtagcc ttacaacatt gccggatgcg tgccaaccgt
    15481 aggtcggata agacgcggca gcgtcgcatc cgacattgaa ggataagacg tgtcaacatc
    15541 gcattcgaca ttgaatgaac gcagaaaagc aaaaagctcg ccgaagcgag cttttttaat
//
LOCUS       DADLTI010000098        15526 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLTI010000098 DADLTI010000000
VERSION     DADLTI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.098"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="JN755_002707"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="JN755_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7381455.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="JN755_002708"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="JN755_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7381456.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="JN755_002709"
     CDS             complement(941..1408)
                     /locus_tag="JN755_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7381457.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="JN755_002710"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="JN755_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7381458.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="JN755_002711"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="JN755_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7381459.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="JN755_002712"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="JN755_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7381460.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="JN755_002713"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="JN755_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7381461.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="JN755_002714"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="JN755_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7381462.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="JN755_002715"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="JN755_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7381463.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="JN755_002716"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="JN755_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7381464.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="JN755_002717"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="JN755_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7381465.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="JN755_002718"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="JN755_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7381466.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="JN755_002719"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="JN755_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7381467.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="JN755_002720"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="JN755_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7381468.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="JN755_002721"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="JN755_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7381469.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="JN755_002722"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="JN755_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7381470.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="JN755_002723"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="JN755_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7381471.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="JN755_002724"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="JN755_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7381472.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="JN755_002725"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="JN755_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7381473.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="JN755_002726"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="JN755_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7381474.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="JN755_002727"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="JN755_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7381475.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="JN755_002728"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="JN755_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7381476.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="JN755_002729"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="JN755_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7381477.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="JN755_002730"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="JN755_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7381478.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="JN755_002731"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="JN755_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7381479.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="JN755_002732"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="JN755_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7381480.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="JN755_002733"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="JN755_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7381481.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="JN755_002734"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="JN755_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7381482.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="JN755_002735"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="JN755_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7381483.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="JN755_002736"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="JN755_002736"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7381484.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="JN755_002737"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="JN755_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7381485.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4003 a         3647 c         4132 g         3744 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac tgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgtat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaag aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atatgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgacccg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctggggcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggtga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DADLTI010000099        15410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLTI010000099 DADLTI010000000
VERSION     DADLTI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.099"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1175)
                     /locus_tag="JN755_002738"
     CDS             complement(<1..1175)
                     /locus_tag="JN755_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7381486.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="JN755_002739"
     tRNA            complement(1374..1458)
                     /locus_tag="JN755_002739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="JN755_002740"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="JN755_002740"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7381487.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="JN755_002741"
     CDS             2802..4304
                     /locus_tag="JN755_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7381488.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="JN755_002742"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="JN755_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7381489.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="JN755_002743"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="JN755_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7381490.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="JN755_002744"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="JN755_002744"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7381491.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="JN755_002745"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="JN755_002745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7381492.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="JN755_002746"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="JN755_002746"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7381493.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="JN755_002747"
     CDS             complement(11869..13065)
                     /locus_tag="JN755_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7381494.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="JN755_002748"
     CDS             13258..13761
                     /locus_tag="JN755_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7381495.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="JN755_002749"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="JN755_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7381496.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="JN755_002750"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="JN755_002750"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7381497.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3971 a         3932 c         3969 g         3538 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctacttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gcaagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggaccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgtg
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcat
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgctcggc cagctcggta atcgtgttca gcacgctttg cgagctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcggtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgttgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc atgactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaatc
    12361 taaaaatgtg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgctgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa taaagtcaaa acacagccaa caagcggcat
    12781 acctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggctcgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat catacacata
    14341 aatttaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggtgct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgagtctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DADLTI010000100        15298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLTI010000100 DADLTI010000000
VERSION     DADLTI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.100"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..210)
                     /locus_tag="JN755_002751"
     CDS             complement(<1..210)
                     /locus_tag="JN755_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381498.1"
                     /translation="MRQTIRNRSGCTAENVNQARIKSLMKPLSSPLQQYWQTVVERLP
                     ELLAEESLSAQAKSVLTFSDFVQDSV"
     gene            complement(161..1462)
                     /locus_tag="JN755_002752"
     CDS             complement(161..1462)
                     /locus_tag="JN755_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7381499.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            1704..2324
                     /locus_tag="JN755_002753"
     CDS             1704..2324
                     /locus_tag="JN755_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7381500.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            2388..3626
                     /gene="cca"
                     /locus_tag="JN755_002754"
     CDS             2388..3626
                     /gene="cca"
                     /locus_tag="JN755_002754"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7381501.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(3789..4610)
                     /gene="bacA"
                     /locus_tag="JN755_002755"
     CDS             complement(3789..4610)
                     /gene="bacA"
                     /locus_tag="JN755_002755"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7381502.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(4701..5069)
                     /gene="folB"
                     /locus_tag="JN755_002756"
     CDS             complement(4701..5069)
                     /gene="folB"
                     /locus_tag="JN755_002756"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7381503.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            5174..5791
                     /gene="plsY"
                     /locus_tag="JN755_002757"
     CDS             5174..5791
                     /gene="plsY"
                     /locus_tag="JN755_002757"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7381504.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(5804..6735)
                     /gene="ttdR"
                     /locus_tag="JN755_002758"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(5804..6735)
                     /gene="ttdR"
                     /locus_tag="JN755_002758"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            6942..7785
                     /gene="ttdA"
                     /locus_tag="JN755_002759"
                     /pseudo
     CDS             6942..7785
                     /gene="ttdA"
                     /locus_tag="JN755_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            7782..8387
                     /gene="ttdB"
                     /locus_tag="JN755_002760"
     CDS             7782..8387
                     /gene="ttdB"
                     /locus_tag="JN755_002760"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7381505.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            8435..9832
                     /locus_tag="JN755_002761"
     CDS             8435..9832
                     /locus_tag="JN755_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7381506.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(9875..10888)
                     /gene="tsaD"
                     /locus_tag="JN755_002762"
     CDS             complement(9875..10888)
                     /gene="tsaD"
                     /locus_tag="JN755_002762"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7381507.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            11126..11341
                     /gene="rpsU"
                     /locus_tag="JN755_002763"
     CDS             11126..11341
                     /gene="rpsU"
                     /locus_tag="JN755_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7381508.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            11452..13197
                     /gene="dnaG"
                     /locus_tag="JN755_002764"
     CDS             11452..13197
                     /gene="dnaG"
                     /locus_tag="JN755_002764"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7381509.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            13392..15233
                     /gene="rpoD"
                     /locus_tag="JN755_002765"
     CDS             13392..15233
                     /gene="rpoD"
                     /locus_tag="JN755_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7381510.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         3713 a         4160 c         3942 g         3483 t
ORIGIN      
        1 cacgctgtcc tgcacaaaat cactgaaagt aagtactgac ttcgcctgtg cactaagtga
       61 ttcctcggct aaaagctctg gcagccgctc aacaacggtc tgccagtact gctgtaacgg
      121 tgaagagagc ggcttcataa gcgattttat ccttgcctga ttaacgtttt ccgctgtgca
      181 accagaacgg ttcctgattg tttgcctcat tacggaaatg ttcaatttca atgcgttgcc
      241 cggtcgcaat agcgtgacgc agcccctgcc agttctccag ccaggcttgc gcgacgacag
      301 gatcatagta acccgccagc aacagtattg agtcaatatc acgcatcagg cgtggcaact
      361 ggtcacggta gcgatcgcct aacggctggc agaaaacgct tttcagttca gcggcatggc
      421 gggaaagatg gatatcggca aagcgtttga aggagtcgct aattttgccc tgcgctttgg
      481 catctaaaaa cggctgccat gctttgctta ccagccattc ggtcaacgcc agcttcgcca
      541 ttgccgtttc ggtagagtag accgccgtca cggcagaaac cgcagaagcg atggtcgctt
      601 cgcattgagt cagcaaatca cgtaagtgag tgctcgcttt acgcggcaca atgccaccga
      661 acagcatcag tgtatgacgg actaggctaa tggctgccag cacctgctct ttcgccgcat
      721 cgttgccgcg tacccacagt tcttcatgat actgccattg cgctaacacc agctcgagcg
      781 ccgcttccag cccctgttcc acatcggctt ttgccgcaac atgcaaaatg gtggtcggtt
      841 tgatttcacg cgccggattg ccctgcgcca gatgatagcc acgcgccgct ttgctcaggc
      901 tgccctggcg taatccggtt tgcgatacca gttggttcgc cagtttcagc accgcgcgcg
      961 tgtcgccgct aagcagctcc agttccagct cgcagatagg ctcagcaaat tcacccgctt
     1021 tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc atcgatctct actagccatt
     1081 tttcgcgata aaaatcggtg ctaaacagcg gctgcacgcg ggaagcgaga tcggcaggca
     1141 attcgccgtt cggccagatt tccgtcggta actgcgccag gtcgagcgtc ggttcgctca
     1201 acgccacgtt atattccggg cgctggtgta agccgcctgt cactcttcct gcaactttca
     1261 tggtcatctc atagcgaccg ttttcgccac gaatacgtaa gcccatatcg tgcctacgca
     1321 gccagttatc cggcgtttcg tagtaaatat tcagcaactg cacggggtca tggtgctcgc
     1381 cgcccagcgt attgagatgg tcacgcaacg cctcaacggc actgtgatta acaataaact
     1441 ttaattcgat ttcctgagcc atggccttta cttatcggtt atgtcacata agtgacgatg
     1501 aacggcgaac ttaatgcgat cttttttgtc agtagatagt attttgcgcc aaattgccat
     1561 gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg acacagagga
     1621 ttccgattga tgtcgaatgc tttgcgtagc gacactgatg ccactactat cgttccactt
     1681 tccatgacaa taacgacagc ctgatgccaa aattacgcct gatcggatta actttactcg
     1741 cacttagcgc gactgccgtc tcacacgctg aagaaacgcg ctatgtttct gacgaactga
     1801 atacctgggt ccgtagcggt ccgggagatc attatcgcct cgtgggcacg gttaacgccg
     1861 gcgaggaagt gaccttatta caaactgacg ccaacaccaa ttatgcccag gtgaaagaca
     1921 gctctggccg taccgcctgg atcccgttga aacaacttag cactgagcca agcctgcgct
     1981 cccgtgtgcc agatctggaa aatcaggtca aaaccctgac cgataaactc accaatatcg
     2041 ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag agcgacagcg
     2101 tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga
     2161 aaaaggtcga tgccgccagc gtacagctgg atgacaaaca gcgcaccatc atcatgcaat
     2221 ggtttatgta tggcggtggc gtgctggggc ttggcttgct gctcggtctg gtactgccgc
     2281 acctgatccc aagccgcaaa cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg
     2341 cactgtctta ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg aagatttatc
     2401 tggtcggtgg tgctgttcgg gatgcattgt tagggctatc ggtcaaagac agagattggg
     2461 tggtggtcgg cagtacgcca caggagatgc tcgacgcggg ctaccagcag gtaggccgcg
     2521 attttcctgt gtttctgcat ccgcaaacgc atgaagagta tgcgctggca cgtaccgaac
     2581 ggaaatccgg ttccggttac accggtttta cttgctatgc cgcaccggat gtcacgctgg
     2641 aagatgatct taagcgtcgc gatctgacca ttaatgcgct ggcccaggac gataacggcg
     2701 agattatcga cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt
     2761 cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt ggcgcgtttt gctgcgcgtt
     2821 atgcccacct cggttttcgt attgccgatg aaactctgac gctgatgcgc gagatgaccc
     2881 atgcgggtga actggaacac ctgacgcctg aacgggtatg gaaagagacg gaaagcgccc
     2941 tcaccacccg caatccccag gtgttcttcc aggtactgcg cgattgcggc gcacttcgcg
     3001 ttttattccc ggaaattgac gcactgtttg gcgttccggc acctgccaag tggcatccgg
     3061 aaatcgacac gggtattcat accttaatga cgctctcaat ggcggcgatg ctgagtccgc
     3121 aggttgatgt ccgtttcgca actttatgcc acgatctcgg taaaggactg acgccgccag
     3181 aactttggcc gcgtcatcat gggcatggcc cggcgggtgt taagttagtg gaacaactat
     3241 gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc cagactggtg gctgagtttc
     3301 acgatctcat ccacaccttc ccaatgctga acccaaaaac catcgtcaaa ttatttgatt
     3361 ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg
     3421 acgtgcgcgg cagaaccggt tttgaatcag cggactaccc gcaaggccgt tggttgcgcg
     3481 aaggctggga agtggcgcag tcagtgccga caaaagcggt cgttgaagcg ggatttaaag
     3541 gcgtggagat tcgcgaggag ctgacccgac ggcggattgc ggcggtagcc agctggaagg
     3601 aacaacgttg cccaaagcct gactgagtat aaaactaaac gttagcacga accgtcccct
     3661 cgccccttcg gggagagggt tagggtgagg ggaacagacc agcaccggtg cgaacaatca
     3721 cccccacccc ggccctctcc ctacagagcg agggagaaaa cccggcctgc tcccgaacct
     3781 tttctcaatc aaaagaacac gacatacacc gcagccgcca caataaagcg ataaatggcg
     3841 aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc
     3901 accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccggt tgtcaggaag
     3961 ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggtac cgccagcagg
     4021 aacgagaact cggaagcagc gtaacggctc acgcccatca gcatcccacc tgaaatggtc
     4081 gccccggaac gggagaaacc cggccacagc gccagacact ggaaacagcc aatcataaac
     4141 gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat
     4201 tcggcggcaa tcagcaacaa accgccaacg accagcgcat acatcacatt tatcgggtta
     4261 aacaatgact taatcgtgtc gtggaataat agccccagta ccaccgccgg aatcatcccc
     4321 agcaaaatgt ggatcagcgt taaacgacct ttgctttcac cttcgtgctg caacgggcgg
     4381 ccaaagtgga tgccaatcag gccaaacaga cgccgccaga acatcactac taccgccaga
     4441 attgatccta actggatcac aacttcaaag gttttcgccg tgtcgccctc aaaccccaac
     4501 aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc tgtcaatcct
     4561 tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat caataaaccc
     4621 ctaaattatt aaaatgtacc gcttgtccga actactgcag tatgaccagg ttataaccgt
     4681 ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg ctcaatgatt
     4741 acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat acgcacccac
     4801 ggcgagttga agcgtgctaa cagcagctcc gccacctctt cagccacgcg ttccaccagc
     4861 gcaaaacgcg ccccctcgac gtggctgacc accgtttctg caatgtcagc gtaactgagg
     4921 caatccgcca catcatcact tttcgccgct ttacggttat cccatgccat ttcgatatcg
     4981 aacactaact tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc
     5041 gaaagttgct ctataaatac aatatccatc acgtcctgcc tgctttttgg ctaaccggat
     5101 accacttccg gcgaaatgtg cgtattatcc acagattcat cgttgaacac gaattttcaa
     5161 aacggaacag cttatgagtg caatcgcgcc tggaatgatc ctcatcgcgt acctctgcgg
     5221 ctccatttcc agtgccattc tggtttgccg cttgtgtggg ctgcccgatc cgcgaaccag
     5281 cggctccggc aatccaggcg caaccaatgt gttacgtatc ggtggcaagg gagcagccgt
     5341 agcagtactg attttcgacg ttctgaaagg aatgttgcct gtctggggcg cgtatgaatt
     5401 aggtgtcagc cccttctggc taggcttaat tgccatcgcc gcctgtcttg gacacatctg
     5461 gcccgttttc ttcggtttta aaggaggaaa aggcgttgct accgcttttg gagcaatcgc
     5521 accaattggc tgggatctca ccggagtaat ggcgggaacc tggttactga ccgtgctatt
     5581 gagcggatac tcgtcgctgg gagcgattgt cagtgcactg attgctccgt tttatgtctg
     5641 gtggtttaag ccacaattca ccttcccggt ttcgatgctc tcttgcctga tcctgctgcg
     5701 tcatcatgac aacatccagc gtctgtggcg tcgtcaggag acaaaaatct ggacgaaatt
     5761 caaaagaaag cgcgaaaagg atcccgagtg atttctggtg gatctacatg acctgatagc
     5821 cttcatcggg cttgcccagc cgttgctggc accatgccgc cagaaattcc acgcagacac
     5881 gaatttcatg ctgcgataga gcggctcccg gtaaacagcc cagatattgg cgctttgtgc
     5941 atactccggc aatacttgca ccagtttgcc actctccaga aacggcaaca catcccactc
     6001 ggaacgcagc ataatccctt tgccctccag cgcccattgc aacactatct cgccgctatt
     6061 ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc tgaccgttcc ccaactccca
     6121 tattccgtgg gtcatatcgc gctctttggt caccagacag tcatgacgac ttaattcctg
     6181 taatgattgt ggctgcggat atttttgcag atactcaggt gctgcgcata atattctttt
     6241 atttttcgtt aacagatgcg caatataata atcaggaatt tcgtcattaa tacgaatatc
     6301 cagatcaata ttatcctgca ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg
     6361 taactcagga taattgcgca taagttcggt aatggctggc gcaatatggc tgcgcccaaa
     6421 accaaaactg caaccaatac gaatcatccc ctccgggcgc gttttgattt gcgtgacgtc
     6481 atcgaccagt cgctgatatt gggtgaggat ttccagcgca tgttcgtaac aacgttggcc
     6541 actttcagtc agcgccacgc cgcgagcgga gcggttcagc agcgttgttg ccagcgtatt
     6601 ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt tgcccaagcg tcgccgctgc
     6661 agcactaaaa ctcccgctgt ggacgatttc taccagtacc tgcaagtctt tggctaaagg
     6721 ccagctattc agcatcgtta attatccgca gttgtgataa gcgcagtgta tttcgcaaaa
     6781 cattgccatt ccctgctctg gcgcaatatt cattcttatt ttcgcgttga ttcacgaact
     6841 gttaattaca tattcacggt ggcaaaaaat ataaaaccac atttttagtg gtagtttgtg
     6901 gcggtgaatt tttccagaca aatacaaaaa ctggagttgc catgatgagc gaaagtaata
     6961 agcaacaggc agtgaataag ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta
     7021 cccgaatgcc tgatgacgtg gtggataaac taaaacagct aaaggatgcc gaaacgtcgt
     7081 cgatggggaa aattatctac catacgatgt tcgacaacat gcaaaaagcg attgacctga
     7141 atcgtcctgc ctgtcaggac accggggaga ttatgttctt cgttaaagtc ggttcccgct
     7201 tcccactgct tggcgagctg caaagcatac tcaaacaagc cgtggaagag gcaaccgtca
     7261 aagcgccact acgtcacaat gcggtagaaa tttttgacga agtaaacacc ggcaaaaata
     7321 ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc cgacaatgac gatgcggaaa
     7381 tcgaagttta catggcaggc ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc
     7441 cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga aaatatctcc accctcgccg
     7501 taaacgcctg tccaccggta ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg
     7561 ccgtactctc gcgtaaagcc attctgcgcc cgattggcag ccgccatccc aatccaaaag
     7621 cggcagaact ggagctacgc ctggaagaag gactcaaccg tctggggatt ggtccacaag
     7681 ggctgaccgg caacagttca gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc
     7741 gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa
     7801 ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg
     7861 gtacgctggt gacctgccgc gacgtttgtc accgccgttt gatcgaactg aaacgtccga
     7921 tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg
     7981 gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg
     8041 aacgtgaatt tattgagcag accggcgtaa aactggtggt tggcaaaggt ggtatggggc
     8101 cgctgaccga agaaggctgc cagaaattca aggcgttaca tgtgattttc ccggcaggct
     8161 gcgcggtgct ggcggcaacc caggtagaag agattgaaga agtgcactgg acagaactcg
     8221 ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg attgtctcta
     8281 ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg
     8341 atcccatcgt ggaagagatc tgcgagcatg tccattacat caaataaccc tcccggagag
     8401 gctcacccct ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg
     8461 atatcttgca ccgctggcgg tcatcgccat tattgctcta cttccggttc ccgcagggct
     8521 ggagaaccat acctggctct actttgccgt ttttactggc gtgatcgttg gactgatcct
     8581 cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc tccatcatcg ccatactctc
     8641 tccctggctg ctgttcagcc ccgaacagct cgcccagcca ggctttaagt tcaccgccaa
     8701 atccctctcc tgggcagttt ctggtttttc taattcggtt atctggctca ttttcgccgc
     8761 ctttatgttt ggcacaggct atgaaaaaac cgggcttgga cgccgcattg cgctgattct
     8821 ggtgaaaaag atggggcatc gcacgctgtt tctcggctat gcggtgatgt tctccgagct
     8881 gatcctggca cctgtaacac cgtccaactc ggcgcgtggt gcgggtatta tctatcccat
     8941 catccgtaac ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat
     9001 tggctcgtac atcatgtgga tggggattgt tgccgactgc gtgaccagcg ccattttcct
     9061 gacggcgatg gcacctaact tgctgttaat tggactgatg aaaagcgcat ctcacgccac
     9121 gctgagttgg ggcgactggt tcctcgggat gttgccgctc agcattttac tgcttctgct
     9181 ggttccctgg ctggcttacg tgctataccc gccggtactg aagtctggtg atcaggtgcc
     9241 gcgctgggca gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat
     9301 gctggggctg atggtaggcg cgctgatgct gcttctgcgc attatcagtt gggacgacat
     9361 tgtcagtaat aaagcggcat ggaacgtttt cttctggctg gcctcgctta tcaccctcgc
     9421 gaccggactc aacaataccg gttttattag ctggtttggc aaactgttag caggcagctt
     9481 aagtggttat tcgccgacga tggtgatggt gacgttgatt gtggtgtttt atctactgcg
     9541 ctactttttc gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgatcgccgc
     9601 cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat
     9661 tggtctgggg agcattctta caccatacgc caccggaccc agtccgattt actacggtag
     9721 tggttatctg ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct
     9781 cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac
     9841 ggtgccggaa attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg
     9901 gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca
     9961 ttccggcata ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca
    10021 cttcgccgcg gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg cggttagcac
    10081 tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg atccaacgct cgcttacact
    10141 taatcatcaa cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct
    10201 ggtcgtcggt gctgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc
    10261 tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac
    10321 cctgagccgc cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg
    10381 cggttttatc aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact
    10441 gaccaatgcc agtcacgctg attaactgcg tatgaccgcc ggacaccagc agcgcgacaa
    10501 acggaaattc cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt
    10561 gtaccgggat tgccggaacg ttccaggcaa acgccagaga gcgccccacg gttgcgccaa
    10621 ccagcagtgc gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg
    10681 ccgttaaacc agactccttt agcgccgcct ggatcaacgg tacggtttta cgcacatgat
    10741 cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc acctgactat
    10801 acaattggtt ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc
    10861 aggaagtttc aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag
    10921 tgtaggacca atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat
    10981 actcctcacc cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag
    11041 caattgcagt aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac
    11101 cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt
    11161 cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt
    11221 tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc
    11281 agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta
    11341 attccccgag agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg
    11401 aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga
    11461 cgaatcccac gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc
    11521 gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac
    11581 aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac agttttacca ctgctttgga
    11641 tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact acgacaagct cgagttcgtc
    11701 gaaacggtcg aagagctggc agcaatgcac aatcttgaag tgccatttga agcaggcagc
    11761 ggccccagcc agatcgagcg ccatcagagg caaacgcttt atcagttgat ggacggtctg
    11821 aatacgtttt accaacaatc tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg
    11881 gaaaaacgcg gattaagcca cgaggttatc gctcgctttg cgattggttt tgcgcccccc
    11941 ggctgggaca acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt
    12001 gatgcgggga tgttggtcac taacgatcag ggacgcagtt acgatcgttt ccgcgaacgg
    12061 gtgatattcc ccattcgcga taaacgcggt cgggtgattg gttttggcgg gcgcgtgctg
    12121 ggcaacgata cccccaaata cctgaactcg ccggaaacag acattttcca taaaggccgc
    12181 cagctttacg gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg
    12241 gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg
    12301 tcgttaggta cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat
    12361 gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg ccgcatggcg agcgctggaa
    12421 acggcgctgc cttacatgac agacggccgt cagctacgct ttatgttttt gccggatggc
    12481 gaagaccctg acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag
    12541 caggcgatgc cactctccgc atttctgttt aacagtctga tgccgcaagt tgatctgagt
    12601 acccctgacg ggcgcgcacg tttgagtacg ctggcactgc cattgatatc gcaagtgccg
    12661 ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat
    12721 gacagccagc ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt
    12781 ccgcagctaa aacgcacgac catgcgtata cttatagggt tgctggtgca aaatccagaa
    12841 ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc
    12901 ttattcagag aactggtcaa cacttgtctc tcccagccag gtctgacctc cgggcaactt
    12961 ttagagcact atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac
    13021 gatatagcag ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt
    13081 gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta
    13141 agcaacgaag aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt
    13201 aacggcttaa gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg
    13261 gcaaatatat aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag
    13321 cgttaacggc tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg
    13381 gataccgtct tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta
    13441 aggagcaagg ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg
    13501 attcagatca gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg
    13561 aagaagcacc ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg
    13621 ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc
    13681 cggtacgcat gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa
    13741 ttgacatcgc taagcgtatt gaagacggga tcaaccaagt tcaatgctcc gttgctgaat
    13801 atccggaagc gatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc
    13861 gtctgtccga tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta
    13921 ccgccactca cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag
    13981 acgaagaaga tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg
    14041 ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt aacgcgtgac accatcaaag
    14101 cgaaaggtcg cagtcacgct gccgctcagg aagagatcct gaaactgtct gaagtattca
    14161 aacagttccg cctggtgccg aagcagtttg actacctggt caacagcatg cgcgtcatga
    14221 tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa
    14281 tgccgaagaa aaacttcatt accctgttta ccggcaacga aaccagcgat acctggttca
    14341 acgcggcaat tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag
    14401 tgcatcgcgc cctgcaaaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc
    14461 aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga
    14521 aagagatggt tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc
    14581 gtggcttgca gttccttgac ctgattcagg aaggtaacat cggcctgatg aaagcggttg
    14641 ataaattcga ataccgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc
    14701 aggcgatcac ccgttctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga
    14761 ttgagactat caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg
    14821 aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag
    14881 tgctgaagat cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt
    14941 cgcatctggg ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca
    15001 ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg cgtgaagcaa
    15061 aagttctgcg tatgcgtttc ggtatcgata tgaacaccga ccacacgctg gaagaagtgg
    15121 gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca
    15181 aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt cctggacgat taatcggtag
    15241 gccggatcag gcgttacgcc gcacccggca ctagtctctc tgcacaaacg ccaccttt
//
LOCUS       DADLTI010000101        15026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLTI010000101 DADLTI010000000
VERSION     DADLTI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.101"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..236
                     /locus_tag="JN755_002766"
     CDS             <1..236
                     /locus_tag="JN755_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7381511.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="JN755_002767"
     CDS             246..884
                     /locus_tag="JN755_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7381512.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="JN755_002768"
     CDS             887..2050
                     /locus_tag="JN755_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7381513.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3800
                     /locus_tag="JN755_002769"
                     /pseudo
     CDS             2134..3800
                     /locus_tag="JN755_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3874..4149)
                     /gene="yjfN"
                     /locus_tag="JN755_002770"
     CDS             complement(3874..4149)
                     /gene="yjfN"
                     /locus_tag="JN755_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7381514.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4298..4627)
                     /gene="bsmA"
                     /locus_tag="JN755_002771"
     CDS             complement(4298..4627)
                     /gene="bsmA"
                     /locus_tag="JN755_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7381515.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4809..5558
                     /gene="yjfP"
                     /locus_tag="JN755_002772"
     CDS             4809..5558
                     /gene="yjfP"
                     /locus_tag="JN755_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7381516.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5555..6310)
                     /gene="ulaR"
                     /locus_tag="JN755_002773"
     CDS             complement(5555..6310)
                     /gene="ulaR"
                     /locus_tag="JN755_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7381517.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6418..7482)
                     /gene="ulaG"
                     /locus_tag="JN755_002774"
     CDS             complement(6418..7482)
                     /gene="ulaG"
                     /locus_tag="JN755_002774"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7381518.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7837..9234
                     /gene="ulaA"
                     /locus_tag="JN755_002775"
     CDS             7837..9234
                     /gene="ulaA"
                     /locus_tag="JN755_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7381519.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9250..9555
                     /gene="ulaB"
                     /locus_tag="JN755_002776"
     CDS             9250..9555
                     /gene="ulaB"
                     /locus_tag="JN755_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7381520.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9565..10029
                     /gene="ulaC"
                     /locus_tag="JN755_002777"
                     /pseudo
     CDS             9565..10029
                     /gene="ulaC"
                     /locus_tag="JN755_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10043..10693
                     /gene="ulaD"
                     /locus_tag="JN755_002778"
     CDS             10043..10693
                     /gene="ulaD"
                     /locus_tag="JN755_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7381521.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10703..11539
                     /locus_tag="JN755_002779"
     CDS             10703..11539
                     /locus_tag="JN755_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7381522.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11539..12225
                     /locus_tag="JN755_002780"
     CDS             11539..12225
                     /locus_tag="JN755_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7381523.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12354..12629)
                     /locus_tag="JN755_002781"
     CDS             complement(12354..12629)
                     /locus_tag="JN755_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7381524.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12956..13351
                     /gene="rpsF"
                     /locus_tag="JN755_002782"
     CDS             12956..13351
                     /gene="rpsF"
                     /locus_tag="JN755_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7381525.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13358..13672
                     /gene="priB"
                     /locus_tag="JN755_002783"
     CDS             13358..13672
                     /gene="priB"
                     /locus_tag="JN755_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7381526.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13677..13904
                     /gene="rpsR"
                     /locus_tag="JN755_002784"
     CDS             13677..13904
                     /gene="rpsR"
                     /locus_tag="JN755_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7381527.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13946..14395
                     /gene="rplI"
                     /locus_tag="JN755_002785"
     CDS             13946..14395
                     /gene="rplI"
                     /locus_tag="JN755_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7381528.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14466..15023)
                     /locus_tag="JN755_002786"
     CDS             complement(14466..15023)
                     /locus_tag="JN755_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7381529.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3509 a         3662 c         4049 g         3806 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaggccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcaattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg ggggagtgtg tgtgtaagcg tatacgactg atgcgacgct
     3781 ggtttcgatt aactaaatga aatatgtgaa aattgtaggc cggacaaggc gctcgcgccg
     3841 catccggcat tgttcatttg ctgtagatga cactcatgca tacagtatcg cctgtacgcg
     3901 ccagttatca ggctgctggt cttcgtacat ggtaataatg cggtaccatg acgcaccttg
     3961 ttcatcggcc tttaacgcca cgatacgttc cacgtcctgc ggatcccctg aaatattgct
     4021 aactgagatc tgacctattt cattcaggcc cgtcacgcag tcagcactgg cgaattctgc
     4081 agactgtgcc gtggcgctcg caggataagt cactaattgc agcgagggtg aggcaagtaa
     4141 ttgtttcatc gtcagctcca tttttcgttt ccccacatgt tgcagggcat tccatcgcgg
     4201 taataaacgc ccaagcttcc agccggtaat ttattgtgca caggtaaacg catagggacg
     4261 caaagcaatg taaagctgac tatgtaagtt gtctgattta tttacggtac aaaatggctt
     4321 gtgaatacca ctgtcctgtc acgatggttt cgtccaccat gacgacaacg taataatcag
     4381 cttttgcagc gacagctttt gctctgattt ctgctaatgc gtcatccgga gaaccccgaa
     4441 ccatcgtgct tacgctacct attcgctgta acccttgcgt ctggtcgcgg cgaatctctt
     4501 gcggatggtc cgttactggc ggtgctggct gtggcgtacc ttgcagtgcg ctacaggcac
     4561 ttaacatcaa caccaataat aaactggcaa accggtaaat aacgctatta cgtttcctgc
     4621 taaccatagt gtagtgcctt attaatttaa ctttggggga atgttcccga attgttacct
     4681 ggtacgcgat tttcgaatga aaacgtgacg aaagcgtaac ccacatctca acattatgaa
     4741 ctaataccgc cagcagaaac ttgtgctaag gatttcttca tgtcatacca gacaaaaagg
     4801 agagacagat gattgaaata gaatcacgcg agctggcaga tattcccgtt cttcatgctt
     4861 atcctgtcgg gcaaaaagat accccgttac cgtgcgtaat tttttatcac ggctttactt
     4921 catccagcct ggtgtatagc tattttgccg ttgcgctggc gcaggctggt ttgcgggtga
     4981 tcatgccgga tgcgcccgat cacggtagcc gttttagtgg cgacgaagcg cggcggttaa
     5041 atcaattctg gcaaatcttg ctacaaagta tgcaggaatt cactacttta cgtgcggcaa
     5101 tagccgaaga aaactggctg cttgatgacc gtctggcagt cggtggcgcg tcgatgggcg
     5161 cgatgacggc actgggaatt accgctcgcc accccacggt gaaatgtacc gccagcatga
     5221 tgggatcggg ctattttaca tcgctcgccc gttcactgtt tccaccgctg atacctgaaa
     5281 cggcagcaca gcagaatgaa ttcaataaaa ttgtcgcgcc gctggcagag tgggaagcga
     5341 caaaccacct agaacaactt ggggacagac ctctactgct gtggcatggc ctcgacgatg
     5401 acgttgtgcc tgccgacgaa tcactacgtt tgcagcaggc cttaagcgag acgggacggg
     5461 ataaactgct aacctgttca tggcagccag gcgtgcgtca ccgcattacg cctgaggcgt
     5521 tagatgctgc cgtgacattt ttccgccagc atctttaaac acgcagaatg ctgacccctt
     5581 gcgcttccag ttgttgcagg atttccgggt tagcattttt gccggtgatc agcatatcga
     5641 tttgatcggc acggctaaaa agcattcccg cgcgttcgcc aatcttactg ctatcaacca
     5701 gtaccaccag tttccctacc acgctcagca tcttctgctc tgccattgct gtcagcatat
     5761 cggttttata caacccttct gcggtcagcc cttttccgct ggtaaacatc cagtgcccgg
     5821 catagagact gttttcgctg ccctgcgggc ttaaagtgat ggactgactt ttgttgtact
     5881 gtccacccat aatgatcacg ctgtcatgtt cttgatcgat caggtaattt gccagcggta
     5941 gataattagt gatgatttgc actggcttgc cacacatttc ccgcccaagc agaaacgcgg
     6001 tggagccgca gttgatgact acgctttcgc ccggattaac cagctgcgag gccgctttag
     6061 caatacgtac tttttcatcg tgattctgcg cctgatgcag attcatcggc gtccagcgcg
     6121 ggcgctgttg ggtaatagct tctgcgccat tgcgcacctt tttcagtttg ccgctttcgc
     6181 caagtttatt gatatcgcgt cgcgcagtgg caggcgaaat tcccagacgc tcaacgactt
     6241 tctcaacggt cacaaagccc aattgtgcga gcatttccag gaggatttga tgtctttgtg
     6301 cttcagtcat gagctattcc gataaaattt gattttttta gatgatattt gaaatagcca
     6361 ggaaatacta cgccggatag cgcgaacttc tccacgctat ccggtataaa cgacagatta
     6421 caggaatgac ttgaacggca gatccggttc aatagtgaag caatcatcga aaccgcgcgg
     6481 atagtggtac tcgaagttgt ctttatccag cggccaggta aatttgccgc caacctgcca
     6541 gataaacggc ttgaagccat acttcagacg atcttttttc atctcccaca gcacgcggat
     6601 ctcttgcgga tcggcctgga agtttgacca gatgtcgtgg tggaacggga taaccacttt
     6661 cgcattcagg gcttcaccca tacgcagcat atcggcgctg gtcattttgt cggtaatacc
     6721 gcgcgggttc tcaccatacg atcctaacgc cacatcgatc tgatgttcgt taccgtgctt
     6781 cgcataatag ttagagtagt gggaatcgcc actgtgatat agggaaccgc caggggtttt
     6841 gaacaggtag tttactgcgc gatcgtccat gccatctggc agtacgccag ccgctttttg
     6901 atcggcaggc agggtgatca gtgcagtacg gtcgaaagcg tcaagcgcat gaatttcaat
     6961 gtctttcact tttacaacat cgcccggttt gaccacgatg caacgctctt tcggtacgcc
     7021 ccagccaatc cacaaatcaa cgcaggtttt cggtccaatg aatggtacgt catcagcaca
     7081 gttctgcatc acggcagcag cgacgttaac gtcgatatga tcgttgtgat cgtgagtcgc
     7141 cagtaccgcg tcgatctggc gaatcgcaaa cggatcaaga acaaacgggg tggtgcgtag
     7201 gtttggctgc agttttttca cgccagccat gcgctgcatc tggtgaccct gtttcattaa
     7261 cgggttaccg tgactttgtt tgccagtgcc gcaccagaaa tcaacgcaaa cgttggtgcc
     7321 accttctgat ttcaaccaga tcccggtgca gcctagccac cacatcgcaa atgtgccagg
     7381 ggcgacctgc tcttgttcaa tttcttcatt cagccagcta ccccactccg ggaaagtgct
     7441 caggatccag gattctcggg tgatactttt cactttactc atcgccattt accttcatga
     7501 tagttcaatt cgaatcaata tgtgattggt tttgattaat cctgacacta ttttttcagg
     7561 aaggcaatga ccattttttg actttttgcc agggaagttg ttgttgattt tgagtgtaga
     7621 aagatttaat ggaatgtata gttaaattaa ctaaatgaat ttaaataaat aattttttcg
     7681 ttgtgtgaat cccactctat ccgtgtggaa ttatttgcgg gtcgcgtcac atttaatcat
     7741 aaataatctt gttgtgatta cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg
     7801 cggaataccc ggttacctct tcttcaggag atcgttatgg agatcctcta caacatcttt
     7861 accgtgtttt ttaaccaggt catgaccaat gccccgttgt tgctgggtat tgtgacctgt
     7921 ctgggctaca tcctactgcg caaaagtgtc agcgttatta ttaaaggcac gattaaaacc
     7981 ataattggtt tcatgttgtt gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg
     8041 gtggtggcga aaatgtccga agtctacggc attaacggcg caatttccga tacttacgcg
     8101 tcgatgatgg caaccatcga ccgcatgggc gatgcctata gctgggtagg ttacgcggtg
     8161 ttgttagcgc tggcgctgaa catctgttac gtgctgctgc gtcgcattac cggtattcgt
     8221 accatcatgc tgactggtca tatcatgttc cagcaggcgg ggctgattgc cgttacgttg
     8281 tttattttcg gctactccat gtggacaacc attatctgca cggcgattct ggtttcgctc
     8341 tactggggca ttacctccaa catgatgtac aagccgactc aggaagtgac ggacggctgc
     8401 ggtttctcca tcggtcacca gcaacagttt gcatcattaa ttgcctataa agttgcgccg
     8461 ttcctcggca aaaaagagga aagcgttgaa gacctcaaac tgccaggctg gctgaatatt
     8521 ttccacgaca acatcgtctc cacggcgatt gtcatgacca tcttctttgg tgccattctg
     8581 ctctccttcg gtatcgacac cgtgcaggcg atggcaggca aagtgcactg gacggtctat
     8641 atcctgcaaa ccggtttttc cttcgcggtg gcgatcttca tcatcactca gggtgtgcgc
     8701 atgtttgtgg cggaactctc tgaagcgttt aacggtatct cccagcgcct gatccctggt
     8761 gcggttctgg cgattgactg tgcggctatt tatagcttcg ctccgaacgc cgtggtctgg
     8821 ggctttatgt ggggcaccat cggtcagctg attgcggttg gcatcctggt cgcctgcggt
     8881 tcctcgatcc tgattatccc aggctttatc ccgatgttct tctccaacgc caccatcggc
     8941 gtgttcgcta accacttcgg cggctggcgt gcggcgctga agatttgtct ggtgatgggg
     9001 atgatcgaaa tctttggttg cgtctgggtg gtgaaactta ccggtatgag tgcctggatg
     9061 gggatggcgg actggtcgat tctggcaccg ccgatgatgc agggcttctt ctccatcggt
     9121 atcgccttta tggccgtcat cattgtaatt gcactggctt atatgttctt cgctggccgc
     9181 gcactgcgcg cagaagaaga tgcagaaaaa caattggcag aacagtctgc ttaataagga
     9241 gttttgatta tgaccgtacg tattctggct gtgtgtggca acggacaagg cagttccatg
     9301 atcatgaaga tgaaagtgga ccagttttta acccaatcaa acattgacca cacggtaaat
     9361 agctgcgcgg ttggcgaata caaaagcgag ttgagcggcg cggatatcat catcgcttct
     9421 acgcacattg cgggcgaaat caccgtgacc ggcaacaaat acgtggttgg cgtgcgcaac
     9481 atgctctctc ctgccgactt tggcccgaaa ctgctggaag tgatcaaagc gcatttcccg
     9541 caggatgtga agtaaggacg cgccatgaaa ttacatgatt cgctggcgga gaataaatcc
     9601 atccgcctgc aggctgaagc tgagacatgg caggacgcag taaaaatcgg cgttgacctg
     9661 ctggtggcgg cggatgttgt cgagccgcgt tactaccagg cgattctgga tgcggttgaa
     9721 cagcacggtc cgtactttgt gctcgcacca ggcctggcaa tgccgcacgg tcgcccggaa
     9781 gagggcgtta agaaaaccgg ttttgcgctg gtgacgctga aaaagccgct ggagttcaac
     9841 cacgaagata acgacccggt ggatatcctc atcaccatgg cggtggtcga tgccaacacg
     9901 cactaggaag tgggcatcat gcagatcgtc aacctgtttg aagatgaaga aaatttcgac
     9961 cgtttacgtg cctgccgtac cgagcaggaa gtactggatc tcattgaccg caccaacgcg
    10021 gcagcttaag aaggaattga agatgtcatt accgatgttg caagtcgcgc tggacaacca
    10081 gactatggat agcgcctacg aaaccactcg cctgattgcc gaagaagtcg acattatcga
    10141 agtgggtacc attctgtgcg tgggcgaagg cgtgcgtgcg gttcgcgatc tgaaagcgct
    10201 ctacccgcac aaaatcgtgc tggcagacgc caaaattgcc gatgcgggca aaattctttc
    10261 tcgtatgtgc ttcgaagcca acgctgactg ggtgaccgtg atttgttgtg cggatatcaa
    10321 caccgccaaa ggtgcgctgg acgtagcaaa agagtttaac ggcgacgtgc agatcgaact
    10381 gaccggttac tggacctggg aacaggcgca acagtggcgc gacgcaggta ttcagcaggt
    10441 ggtttatcac cgcagccgtg acgcgcaggc cgctggcgtg gcgtggggtg aagccgatat
    10501 cactgcgatc aaacgtcttt ccgatatggg cttcaaagtc accgtcaccg gtggcctggc
    10561 gctggaagat ctgccgctgt tcaaaggcat tccaattcat gtgtttatcg ccggtcgcag
    10621 catccgtgat gccgcttctc cggttgaagc cgcgcgtcag ttcaaacgtt ccatcgccga
    10681 actgtggggc taaggagcgg atatgttgtc caaacaaatc ccgcttggca tctatgaaaa
    10741 agcgctcccc gccggggagt gctggctgga acgcctgcga ctggcaaaaa cgttgggctt
    10801 cgattttgtc gagatgtcgg tagatgaaac tgacgagcgc ctgtcgcgcc tcgactggag
    10861 ccgcgagcag cgtctggcgc tggtgaatgc gattgttgaa accggcgtgc gtgtgccgtc
    10921 catgtgcctt tctgcccatc gtcgtttccc gttgggcagc gaagatgacg cggtgcgggc
    10981 gcaggggctg gagattatgc gtaaagccat ccagttcgcc caggacgttg gcattcgcgt
    11041 gatccagctg gcgggctatg acgtttacta tcaggaagcc aataacgaaa cgcgtcgtcg
    11101 tttccgtgac ggcctgaaag agagcgttga gatggcgagc cgcgcacagg tgacgctggc
    11161 gatggagatc atggattatc cgttgatgaa ctccatcagc aaggcgctgg gttacgcgca
    11221 ctatctcaac aacccgtggt tccagctcta cccggatatc ggcaacctgt cggcgtggga
    11281 caacgatgtg cagatggagt tgcaggccgg aatcgggcat atcgtcgcgg tgcatgtgaa
    11341 agacaccaaa ccgggcgtct tcaaaaacgt gccatttggc gaaggtgtgg tggatttcga
    11401 acgttgcttc gaaacgctca aacagagtgg ctattgcggg ccgtacctga ttgagatgtg
    11461 gagcgaaacg gcggaagacc cggcggcaga agtggtgaaa gcgcgcatgg ccaaagcagg
    11521 catggtggag gcggcataat gcaaaagcta aaacagcagg tgtttgaagc caacatggat
    11581 ctgccgcgct atgggctggt gacctttact tggggcaacg tcagcgctat cgaccgcgaa
    11641 cgcgggctgg tggtgatcaa gcccagcggc gttgcctacg aaaccatgaa agcagacgat
    11701 atggtggtgg tcgatatgag cggcaaggtg gtggaagggg agtatcgccc atcttccgac
    11761 actgcgacgc atctcgaact ctaccgtcgt tacccgtcgc ttggcggcat tgtccatacc
    11821 cactccactc atgccaccgc atgggcgcag gcggggctgg cgatcccggc attaggcacc
    11881 acgcacgctg actacttctt tggtgatatc ccgtgtacgc gtggattaag cgaagaagag
    11941 gtgcagggtg agtatgaact gaacactggc aaagtgatta tcgaaacgct gggcaacgcc
    12001 gagccgctgc atacgccggg aattgtggtg tatcagcacg gaccgttcgc ctgggggaaa
    12061 gatgcccacg acgcggtgca taacgcggta gtgatggaag aagtggcgaa aatggcgtgg
    12121 attgcgcgca gtattaatcc acaactcaat catatcgaca gctacctgat gaataaacac
    12181 ttcatgcgta agcacggtcc taacgcttat tacgggcaga agtaaaacac gcgctgcgga
    12241 aatttccttc ctcgggagat aactggtcta attccgcagc cgtttttcaa aaaaagcccc
    12301 ctgcgaaggg ggcaaagcaa actatggcaa tgtttcgttg gttatacctg gtgctagcga
    12361 taaatatccg cgctggcgtg catattgccg ttactccccg gttcccgcat cagaattacg
    12421 tggtagtacg ttgcgccttg cgcatctgtt tcttcattta atgcctgacg tgcttcgctt
    12481 tcagtggcga aattatgatt gatataaatc acgcctaagc tttgcacatc gtccatattt
    12541 ctggcctggt ggttattaat ttcaatggct gcccatgtat ttgcacttag caaaagcaca
    12601 gccagaaggg ctaaaactcg actgaacata gatacctcct cgacggctga ctttgtgtgc
    12661 tctcctctgt gatgatcttc tgatttaatt ttaatcaatg ataaagaagt tgatggtgac
    12721 catttctgat gcagttgttc aaaaaaacac tgtgacggaa tgtgatgaac ttcaaatcag
    12781 cggtttagag gttatttgcg aaaggggaga tttatttctg ctccgccctt gagtttagcg
    12841 gggtatgtca gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc
    12901 cccgggattc ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg
    12961 tcattacgaa atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat
    13021 cgagcgctac actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg
    13081 gggccgccgt cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat
    13141 gaacattgaa gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga
    13201 tgccgttatc cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat
    13261 ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga
    13321 tgatgctgaa gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc
    13381 cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca
    13441 gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcatggtg
    13501 tcaaatgccc gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt
    13561 cggcagtcgc ataaccgttc aggggttcat ttcatgccac aaggcaaaga acggactgag
    13621 caaaatggtt ttgcacgccg agcagattga attgatagat tctggagact agccatatgg
    13681 cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg
    13741 actataaaga tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa
    13801 gccgtatcac cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg
    13861 ctcgctacct gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt
    13921 aatacgactt tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct
    13981 gggtagcctg ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc
    14041 gcagggtaaa gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc
    14101 tgaactggaa gctaaactgg ctgaagttct ggcagctgct aatgctcgcg ctgagaaaat
    14161 caatgcactg gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg
    14221 ttccatcggt actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa
    14281 gagcgaagtt cgtctgccga acggcgttct gcgtaccact ggcgaacacg aagtgagctt
    14341 ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt
    14401 tattcaacga gacgtaaaaa cgccgaccat tggtcggcgt tttgctttct atttttcgtc
    14461 aggcattaat ttcgcaagta gatccattca ccattctctt gtgtccataa gtcaggatgg
    14521 tttgctggcg ttagatgcat acgatcactc cttatgtctc ggtcctgcgg cactcgctcg
    14581 aaacctaaac ccgagtagat tgcggctgaa tttgataagg aacgtagacc aattttcaaa
    14641 tcatggggta aatcagaata cgcgtttagc aatgcagcaa tgatgaatcc gagggcatta
    14701 gaaacgcctg taacagtgcg aagttggtcg atataaagat cgattgttgg tagttcagat
    14761 gaccagcaat taggcatttg ggcaggaagg gttacaacgt atcctatcgt ttggccggta
    14821 tagtctggag tactataacc aggtcccaaa acaacctcgt gtattgtgta tttactgttg
    14881 ggttcgaagg attcgacatc ttgcaataaa tatttcagtt ctgcggttaa cctcgcaccc
    14941 gcgtaagctt tattgtattt ctgggcgtga atattatcta atgtagcttc atggttccat
    15001 ccaaaagaat tatcaccatt cattgt
//
LOCUS       DADLTI010000102        14955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLTI010000102 DADLTI010000000
VERSION     DADLTI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.102"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..347
                     /locus_tag="JN755_002787"
     CDS             <1..347
                     /locus_tag="JN755_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7381530.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
     gene            344..799
                     /locus_tag="JN755_002788"
     CDS             344..799
                     /locus_tag="JN755_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381531.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JN755_002789"
     CDS             complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JN755_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7381532.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1913..2821
                     /gene="mak"
                     /locus_tag="JN755_002790"
     CDS             1913..2821
                     /gene="mak"
                     /locus_tag="JN755_002790"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7381533.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JN755_002791"
     CDS             complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JN755_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7381534.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JN755_002792"
     CDS             complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JN755_002792"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7381535.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JN755_002793"
     CDS             complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JN755_002793"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7381536.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8803..9492
                     /gene="phoB"
                     /locus_tag="JN755_002794"
     CDS             8803..9492
                     /gene="phoB"
                     /locus_tag="JN755_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7381537.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9550..10845
                     /gene="phoR"
                     /locus_tag="JN755_002795"
     CDS             9550..10845
                     /gene="phoR"
                     /locus_tag="JN755_002795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7381538.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11252..12571
                     /gene="brnQ"
                     /locus_tag="JN755_002796"
     CDS             11252..12571
                     /gene="brnQ"
                     /locus_tag="JN755_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7381539.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12647..14020
                     /gene="proY"
                     /locus_tag="JN755_002797"
     CDS             12647..14020
                     /gene="proY"
                     /locus_tag="JN755_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7381540.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14176..>14955
                     /locus_tag="JN755_002798"
     CDS             14176..>14955
                     /locus_tag="JN755_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7381541.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWF"
BASE COUNT         3316 a         3803 c         4024 g         3812 t
ORIGIN      
        1 tccaggtatt ttttgcggaa ctggctacga taaaagataa atcagatgat gaatggtggc
       61 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattacgc aacgcacata
      121 ccccggcgtc gttgttaacg acattgactg agcctcagga tcggtcgctg gctattaata
      181 atccacagct ggctgccgat gtgaaaacgg cgtggttaaa agaggatcca tcattactct
      241 tatttgtcga acaacccgat ctttcgcagt tacgtgattt agtgaaaacc ggggcaacgc
      301 ggaaaattcg cagtgaagcg cgtcaccggc ttgaggaaaa acaatgacac aacgtccatg
      361 gagcaagtta cagcgggaga tctatgactt actgacgcca acgatcaacc tgcaaattca
      421 ctgtacccgt tatcccatgc gcagccaaaa tggtggaagt accgatttac cgcgttattg
      481 gatcacgttg gataaaaacg tgatctggga ttacccgaaa gactttattg caggtaacgc
      541 tggagtgcgt aattttcacg gtgaaacttg ctggtatcct tacctgacgg atatttgttc
      601 aatttctgat ttattgcgtg aatatattga taccccaaaa gcggagctgt taacaaaaca
      661 atttaccagc gataaatggg ggttagtgaa tattctgcgt gctgctgaca ggcgaatagg
      721 gatgaggcga ttagatcagt tacggcgtaa aacgcataat attgctgcgc taaaaattat
      781 tgctcgccgt agcgaataat tatatgcctg gtgtggcttc gtacgccgga taagacgcgg
      841 caggcgtcgc atccggcatt aaaggaaaat cagcaattaa cgttgtgctt cgccacctaa
      901 tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga tgaaatcggc
      961 atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgt gcagctcgtc
     1021 gcagaactta agacgcttga gcgaaccatc gtcgcacatc acaaactgaa tgcgctgctg
     1081 ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtgattgg tgatctcttc
     1141 gctggtcaga tcttgtttct tcgcgcggat cacgccgcca tcttccagca acgatttcag
     1201 ctcggcttca tcaagcagct ggaagccctg tgccgcacta ccggagcgaa cccattcggt
     1261 cagcgtcagt tcaatcgggt tttccatgct caatggtaca accggtaacg accccaggct
     1321 tttacgcagt aatgccagcg tatcttccgc ttttttggca ctggcgcagt ccaccataat
     1381 caaaccgtta accgtgtcga gccacatcat tgtctggcta aaacggctga aagcacgcgg
     1441 cagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct tcagcttacg
     1501 cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa tcaccggaga
     1561 cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg caacgtgcgt
     1621 taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca tgtcctggct
     1681 gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg cacgcagcga
     1741 aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga taatttccac
     1801 ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt gcattgccaa
     1861 tccggttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta cagtgcgtat
     1921 aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg caggggagca
     1981 gttgtaccgc catcgtctgc ccacgccgcg tgatgattac cggcagacta ttgaaacgat
     2041 cgccacgttg gttgatatgg cggagcaggc gacagggcag cgcggaacgg taggtatggg
     2101 cattccaggt tcgatttcgc cctacaccgg tgtggtgaag aatgccaatt caacctggct
     2161 caacggtcag ccattcgata aagacttaag cgcgaggttg cagcgggaag tgcggctggc
     2221 aaatgatgcc aactgtctgg cggtttcaga agcagtagat ggcgcggcag cgggagcgca
     2281 gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat tcaatgggcg
     2341 gacgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc taccgtggat
     2401 ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta aacaaggttg
     2461 tattgaaacc tttatttcgg gcacgggatt cgcgacggat tatcgtcgtt tgagcggaca
     2521 tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg tagcggaact
     2581 ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg tgaatattct
     2641 cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt tatatcaaac
     2701 ggttgggcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc cggtgagtaa
     2761 ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc cacaagagta
     2821 aaaaacgtag gcaattggcg catcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     2881 tacaaaatgt gccagaaccg cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa
     2941 gcaacgcgca ccgtgacagt aatctacccc agtggtttcg ccagcaccgg actatccgcc
     3001 gcttgctggc gcttatagcg accatacagc agcaacgatg acatcgcagc aaacgaaaac
     3061 agggcggcag gcagtgccac gtaattatat gccagcccca gcgtcagcat catacctccg
     3121 caatatgcgc cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat
     3181 aactctccgc ctttggcatt ttgcagtaac aatatttgca gaggtgctga aagggcaaat
     3241 aatcccgcgc aacaaataaa agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa
     3301 aagagcatca gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt
     3361 gaataacgtc ctgaaatcct gccacttagc atatttccca gcaccatccc tagcccaact
     3421 aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc
     3481 tttacgtagc tgaaccaggc aaacacacct gcgttgccaa acatcgtggc ggcgaaaatt
     3541 aaccacgggg ccgggctgcg caaaaagtga aattgttcgc gcagatttcc tttcgcctcg
     3601 tcgcgaatat ctggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaacagcg
     3661 atcaataaaa aggtgtaacg ccagcattcc tgacttaaat acgttcccag cggaatgccc
     3721 agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg
     3781 ggtttgataa tttttgataa cacgatcgct ccgacgccaa aaaatgcgcc atgcggaaag
     3841 ccggatacca gccgaccaat ggcgagcatc aggtaagacg aagagagcgt gaacatggcg
     3901 ttgccaatga cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg
     3961 gaaaagagtg cgatgattgg cgcaccgacc accaccacca gtgcataata cgagatcata
     4021 tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgctcata
     4081 atgccaaatt cggctatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt
     4141 ttcataccac catcacaacc accagaacgg ctaccaatca ccgtagccaa taaagtgata
     4201 gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc
     4261 tgctgaatag ttatttcact gcaaacgtac tttccagttt gctgtagccc aggccgttga
     4321 tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg tggctaatca
     4381 caccgatggt tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg
     4441 tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat
     4501 ggctgaccag atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc
     4561 cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt
     4621 ccagcgcctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct
     4681 gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat
     4741 cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca
     4801 tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttatctgca tcctgcttca
     4861 gctgctggcg aatctcgcct tgactcgtgg tgttttcacg caacttttgg tgagtttgcg
     4921 ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt
     4981 gttgatgctg tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct
     5041 ggttttccag attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg
     5101 ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg tagcgtggtg tcaaactgcg
     5161 cttgggcttt ttgcagactt tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct
     5221 ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt
     5281 cttcgctgtg cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct
     5341 gaatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt
     5401 gctgacgtgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac
     5461 ccgccaatgc cgttaagagc tgctgctgac gttgttcaat ttgctgttga tactggataa
     5521 tttgctgatt atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca
     5581 gctggcgttc gtgctcatct tgtgcatcca gccacggttg aatatcgtcc tgtggctgca
     5641 aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt
     5701 gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt
     5761 gcccgcgcag cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta
     5821 atcgagactg attaacacca ggctccagcg cctgatacgc ctcgaccgcc gggtggctgg
     5881 tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgctgg gcttccagcg
     5941 ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt
     6001 ctttataacg ctggcgcatt tcgttaagtg cgacattacg ttgcgtctgt tccagagtga
     6061 cattctggat agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat
     6121 gcagcgcgac caggcgctga cgcagtgggc gttgctcggc atgttgcgcc agggcggtag
     6181 caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag
     6241 catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga
     6301 actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta
     6361 accaggtatt caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt
     6421 ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact tcttcaatct
     6481 gctggcgaat atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa
     6541 gatttcgtgc cggttgtgcc agactaagcg ccgccagttg aggttgcgct ttttcttctt
     6601 cggctaacgc ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcct
     6661 gacgcgttaa ccagtttagc gattgttgtt cttgctgctg cgcggtaatt aactgttttt
     6721 cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca
     6781 acgtgacgcc gctggcctgc gcttgcagct tctccagctc agtacgggcc gatttgtgct
     6841 gctcaaaaac catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca
     6901 attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca
     6961 gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct
     7021 tatctttcac tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgag
     7081 gcacctgcaa attaccgtcc ggctggttac gcgcccgatt ctggctccag aacgcgcgat
     7141 acgcttcacc tttcacttca aactccacct ccgccagaca ttcagcggta tcgcgggtca
     7201 tgagatcatt ttgcgattgt gaaacgttag agagacgcgg agtttcgtga tacagcgcca
     7261 gacaaatggc gtccagcagg gtggttttcc ccgcaccggt tgggccggta atggcaaaca
     7321 gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt
     7381 tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg
     7441 caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag
     7501 tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg
     7561 gctggctaac acacgctcgc gctgttcacg actccgacgt accagcaata cttcgacagg
     7621 caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt
     7681 gatttcgata tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg
     7741 tgcggtaatc gacgccagat cgcctttcag caccgccatg ggttgagtta ccggtacgtt
     7801 caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact
     7861 cttaccgcat tcatcaaaac tcagtggaat gggggaaccg caatagcgaa catgttccat
     7921 gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa
     7981 gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt
     8041 actggcaccc acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg
     8101 cagtttgcag gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg
     8161 ctgctgtttt tcaataccgt taagccccgc ctggctggaa ataatgtcac gcgggcgtaa
     8221 aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg
     8281 cgcatgtccg gcgctggcga ccacggttgt attgaggaac gccatgatat cgcgcgattc
     8341 attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg
     8401 ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt
     8461 atcgaaaaca tcaccagcaa caataatcgc atccacctga tgggtttgtg ctgtctccag
     8521 cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc
     8581 gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggt
     8641 cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac
     8701 ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt
     8761 cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg
     8821 gtcgtagaag atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc
     8881 tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg
     8941 ccggatttaa ttctccttga ctggatgtta cttggcggct ccggtatcca gttcatcaaa
     9001 cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg
     9061 gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagcca
     9121 ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg
     9181 gcggtggaag aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg
     9241 atggcgggcg aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt
     9301 atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt ctggggaact
     9361 aacgtgtatg tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg
     9421 gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca
     9481 acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga
     9541 gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc
     9601 tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca
     9661 tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg
     9721 tgggtggatc gcagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac
     9781 ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt
     9841 aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac cacggaagag
     9901 ggcggtattt tctggtgtaa cggtctggcg caacaaattc ttggtttgcg ctggccggaa
     9961 gataacgggc agaacatcct taacctactg cgttacccgg agtttacgca atatctgaaa
    10021 acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt
    10081 cgcgtcatgc cttataccca caaacagttg ttgatggtgg cgcgtgatgt cacgcaaatg
    10141 catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg
    10201 ccattgaccg tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg
    10261 gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg
    10321 aagcagttgc taacgctgtc gaaaattgaa gcctcgccga cgcaattgct caatgaaaag
    10381 gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa
    10441 aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa cgaagatcag
    10501 ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg
    10561 catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagcgt tgaagataac
    10621 ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat
    10681 aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg
    10741 gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc
    10801 agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc ctttgtcatc
    10861 ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt
    10921 tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga
    10981 aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc
    11041 ctccttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact
    11101 gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga gtatttcccg
    11161 ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt
    11221 tacaacacca catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat
    11281 catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc
    11341 tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat
    11401 tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt
    11461 tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta
    11521 cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttcct ttgaagtggg
    11581 gattgcgccg ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt
    11641 cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt
    11701 ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgtcgcga ttgtctggcc
    11761 ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcatttt ctaacggctt
    11821 cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt
    11881 taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg
    11941 ggctggcctg atggcgggtg ttggtctgac tttgctgtac ctggcgctgt tctgtctggg
    12001 ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta
    12061 cgttcagcac acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc
    12121 ctgtctggtc acggcggttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt
    12181 accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtgtctaa
    12241 cctcggctta agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc
    12301 gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt
    12361 gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc
    12421 tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct
    12481 ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc
    12541 aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg
    12601 ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa
    12661 taagctaaag cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat
    12721 tggcaccggg ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt
    12781 gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat
    12841 gtcggtacat aacccggccg ccagctcttt ctcgcgttat gcgcaggaaa accttggccc
    12901 gctggcaggt tacattaccg gatggaccta ctgctttgaa atccttattg tcgccatcgc
    12961 cgatgtgacc gcttttggta tctatatggg tgtcttgttc ccgacggtgc cgcactggat
    13021 ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtctt
    13081 cggtgagctg gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt
    13141 cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca
    13201 taacctgtgg agcaacggcg gcttcttcag taacggctgg cttggcatgg taatgtcgtt
    13261 gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc
    13321 gaaagatcct gagaaatcga ttccgcgtgc gattaactcc gtgccgatgc gtattctggt
    13381 gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac
    13441 tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat
    13501 tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt aacagtgacg tatttggcgt
    13561 aggccgtatg ctccacggta tggcagagca gggcagcgca ccgaaaattt tcagcaaaac
    13621 gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc
    13681 ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac
    13741 cttcgccacg gtatgggtgt ggattatgat cctgctgtcg caaatcgcct tccgtcgccg
    13801 tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac
    13861 catcggcggg ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac
    13921 gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt
    13981 taaacgccgc cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg
    14041 gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc
    14101 caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt
    14161 catcacaggg gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa
    14221 caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc
    14281 attatgctgc gtacagaaca cgataacgaa gaaatgtcag taccaatgca taagcagcgc
    14341 agtcagccgc agccaggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa
    14401 ccccggcggc gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg
    14461 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    14521 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    14581 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    14641 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    14701 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    14761 acagcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag
    14821 gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac
    14881 aatacgcagc agctgggaat gcggctggtg ctggacggcg tgtttaacca cagtggcgat
    14941 tcccatgcct ggttt
//
LOCUS       DADLTI010000103        14935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLTI010000103 DADLTI010000000
VERSION     DADLTI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.103"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            84..365
                     /locus_tag="JN755_002799"
     CDS             84..365
                     /locus_tag="JN755_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381542.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JN755_002800"
     CDS             complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JN755_002800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7381543.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JN755_002801"
     CDS             complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JN755_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7381544.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JN755_002802"
     CDS             complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JN755_002802"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7381545.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JN755_002803"
     CDS             complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JN755_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7381546.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JN755_002804"
     CDS             complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JN755_002804"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7381547.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JN755_002805"
     CDS             complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JN755_002805"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7381548.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JN755_002806"
     CDS             complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JN755_002806"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7381549.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7969..9219
                     /gene="icd"
                     /locus_tag="JN755_002807"
     CDS             7969..9219
                     /gene="icd"
                     /locus_tag="JN755_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7381550.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9329..9652)
                     /gene="iraM"
                     /locus_tag="JN755_002808"
     CDS             complement(9329..9652)
                     /gene="iraM"
                     /locus_tag="JN755_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7381551.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(10185..10295)
                     /locus_tag="JN755_002809"
     CDS             complement(10185..10295)
                     /locus_tag="JN755_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381552.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10348..10752)
                     /locus_tag="JN755_002810"
     CDS             complement(10348..10752)
                     /locus_tag="JN755_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY7381553.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10973..11704)
                     /locus_tag="JN755_002811"
     CDS             complement(10973..11704)
                     /locus_tag="JN755_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7381554.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11909..13120)
                     /gene="bluF"
                     /locus_tag="JN755_002812"
     CDS             complement(11909..13120)
                     /gene="bluF"
                     /locus_tag="JN755_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY7381555.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPKEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13434..13670
                     /locus_tag="JN755_002813"
     CDS             13434..13670
                     /locus_tag="JN755_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY7381556.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13713..13985
                     /locus_tag="JN755_002814"
     CDS             13713..13985
                     /locus_tag="JN755_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY7381557.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14014..14280
                     /gene="ariR"
                     /locus_tag="JN755_002815"
     CDS             14014..14280
                     /gene="ariR"
                     /locus_tag="JN755_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY7381558.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14393..14641
                     /locus_tag="JN755_002816"
     CDS             14393..14641
                     /locus_tag="JN755_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381559.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4008 a         3398 c         3481 g         4048 t
ORIGIN      
        1 gcatgaggat tttgcttttg atataagcga cagccaccgc cagaaactgg aagaatttag
       61 cggggtgtaa tgaggtcagc agcatgacag aagccgaaat aacgggatta atccgccgcg
      121 ttgccggaat cagccagcag gctgacgaac aggccacgca gccggacagc gtgatagccg
      181 aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga tggtatagaa cttaatggcg
      241 tggatatgcg caacatacga accagagttc ttgagttgct ggcctaccgt cgccgcgtgg
      301 agatgtatcg ggagaaggag aaaatcacgt accacgggaa gaagccggag cggttacggc
      361 ggtaactcac tgagattaaa gcaaagcgca aaattgcgct ggctacatcc ctcacatctg
      421 aggactgtta accagcaggc cggaatatat tcagaaacta cgaaattttc gcagtttaat
      481 tactcgtcag cttaatcgct aacccgctct gatattttcg gaaacctcaa tttgaggaag
      541 tcggcgaggt aactgtttga tattcccgat agcgcaaaaa tgcgcggtcg ggcagaatga
      601 ccaccacctc aatcgtgacg gagagtgaca accagtgaca caccgtgaca aaaagacggt
      661 ttaaccgttc attaaaaatc gacgtgattt tgtgctttcc ggatgacgtg gccaacgtca
      721 ttttttagcg aaaaattctg attaaggtca ctggctgaac ggtcataatg accacgcaga
      781 ccatcgaggc aaccatagcg atcatcgtta cggttggagc ttttccgcca ttggtggaaa
      841 aggtgcacat ctggatcatc attacggtga tcaagggtat gtaaaaccca ccagcctgat
      901 taaaggttaa ccggaaaaaa aatccggtag ccccgttatg gggatcccca tatcgacatt
      961 aacgcccctc atgaattggt gcgcttcccc ctggaaagat tacccgcctg tatatttctt
     1021 gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt aatggtgtag
     1081 tcaatttgcg atttttggca ctttttcaat gaaaattgtt taatcgaatc aatgagttga
     1141 tgtagtggta aaaggacata tttattcatc tttcggcgca gaatgctggc gaccaaaaat
     1201 cacctccatc cgcgcaccgc ccagcatgct ctctccggcg acgattttac cctcatattg
     1261 ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc ccagggcgta aagtatcaac
     1321 ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat ggaataccgg ggccatcatc
     1381 ctcgaccaca atatagagat gctcgtcggt ttgccttgca gaaatttcga caaactcgag
     1441 gcaatattta caggcattat ccagcacgtt gcccatcacc tcgacaaaat cgttctgctc
     1501 accgacaaag ctgatctctg gcgaaatatc gagagagata ttgacccctt tgcgttgata
     1561 cactttgttc agcgctgagg tgagattgtc cagcagtggg gcgaccggat gcagctcgcg
     1621 gctgagcaat gtcccgccgc gcatactggc acgatgcagg tagtagccaa tttgctgtga
     1681 aatgcggctg atttgctcca gcattaccgg ctcagcatca ctgacgctca tcttttcact
     1741 acgcagagaa cgcagcgtac tttgcagcac cgccagtggc gttttcagac tatgggtcag
     1801 gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt tcacttttta acaatcggtt
     1861 caggtttcgt accagactgg tcagttctcg cgttgtggct ggattgagca attcgcggtt
     1921 atgttcttcc agttcgcgga cttcttttgc cagggcttcg atggggcgta aactccacca
     1981 ggcggcgacc cacagcagcg ggatcactaa cagcagattg gctgagagta catagataaa
     2041 ccagctccag accatatagg aactttttag ctccaccgga atggtatcca ccaccacaat
     2101 ggttaatttt ggcatccgcg atgttgccgg gtagacgttt accgccaccg agtgggtcat
     2161 ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt tgctgtatcg aatgatctcc
     2221 actcagcaag aggctggtat cgttaacatc cgcttcaatt tcatgaaaac cattcgattt
     2281 cagccagtca ggctggatca ttttcatcag ccagggcacg tcacgttgcg cccataaaag
     2341 ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt tgcttgtcga tattttcggg
     2401 taactcgaca tgcaacttat tgttttccca ctgcgcaagg gtatagaaca gattgctctc
     2461 gccacgtaac agccgaaacg tagttttatc gaaactgacg ctataaccga tcagcgcgac
     2521 cattccgtag gcaagcgaaa gcaccagtac taccgctgcc gttgccaaca gaaaacgtac
     2581 ccgcagcgag agcgggaaaa aaagacgcag taattttttc atcagcgcaa ttcgaacagg
     2641 tagccctggc cgcgaacggt ggtaatgact tcttgcggat attgtgcctg aatttttttg
     2701 cgcagacgtc ccatcagtac atcaatggta tggctttccc gcagctccgc atccggatag
     2761 agttgtagca ttaacgaatc tttgctgacc actttgccat tattgcgtat caacgtttcc
     2821 ataatggtgt attcgaacgc ggtcagtttg atcacttcgt cattaataga taattcacgg
     2881 cgagagagat caacctgaaa cggggggagc gaaatgacct gtgaagccag accgctatta
     2941 cgccgcatta atgcctgcat tcgcgccatc acctcttcaa tatgaaacgg tttagtcacg
     3001 taatcatcag caccggcact taatacttcg actttgtcct gccagctttc acgggcggtt
     3061 aataccagaa tcggcagtga aacatcgttg ctacgccagc ggcgaatcag tgacagaccg
     3121 tcctcgtcag gcaatccgag atcgacaatc gcaatatccg gtaaatgttc attgagataa
     3181 taatcggctt ctttggcatc ttctgcgtca tcgacctgat gaccagcatc ctgaatctga
     3241 actttaaggt ggtgacgtaa caacgcattg tcttcaacaa ccagtacgcg catttttatt
     3301 tctccctgtc ttaattatta aaatagtgta acgcgattat gtgtttatgg gggtaaacat
     3361 taaataaacc agcggggagg ggaggtaaag tgaaaaaaga aaaagtggat aatcttaata
     3421 agcaggccgg acagcatcgc catccggcac tgatttgagg cttatttcag ctcatcaacc
     3481 atcgtgatag ctcgaccaat atagttagcc ggcgtcatcg ctttcaggcg ggctttctct
     3541 tcttctggca acgccagacc atcgataaac tgcttcatgc cttcggcgtc aacgcgctta
     3601 ccgcgagtaa gctctttcag cttctcgtac ggtttttcga tgccatagcg acgcataact
     3661 gtctggattg gttctgccag cacttcccag ttgtgatcca gctcatccag cagatggtca
     3721 cggttcactt ccagtttgct cacgcctttc agggtggatt gatacgcaat cagcgcataa
     3781 ccgataccca cgccgaggtt acgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg
     3841 gaaaccggca gtttgcttgc cagatgctgc aataccgcgt tggaaaggcc cagattccct
     3901 tcggagtttt cgaagtcgat cgggttaact ttatgcggca tggtggaaga accaatctca
     3961 ccagcaatgg ttttctgttt gaagtggtta agggcgatat aaccccagac gtcacggtca
     4021 aagtcgatca gaatggtgtt gaagcgcgca acgcaatcaa acagttcggc aatgtagtcg
     4081 tgcggttcaa tctgggtagt gtacgggttc cattgaatac ccagcgaggt gacgaactct
     4141 tcgctgaact gatgccagtc aacttccggg taagcggcga tgtgggcgtt atagttaccg
     4201 accgcgccgt tgattttgcc gaggatctcc acctggttaa gctggcggtg ctggcgctcc
     4261 atacggtagg cgacgtttgc catctcttta ccgatggttg acggcgtggc tggctgaccg
     4321 tgggtacgag acagcagcgg gatatcgcgg tactgaacgg cgagatcttt aatgccatca
     4381 atcagttgac gccagtacgg caggatcact tcatcacgcg cggttttcag cattaatgcg
     4441 tgggagaggt tattgatatc ttccgaagta caggcaaagt ggatgaattc agaaaccgcg
     4501 tgcagttccg ggatctccgc cactttttct ttcaggaaat actcaaccgc tttaacgtcg
     4561 tggttagtgg tacgctcgat ggttttgatg cgtgcggcat cttcttcgct gaaattggcg
     4621 acaattgcat caaggtaacc gattgcgtcg gcagcaaaag caggaacttc cttgatcgct
     4681 gcgtgcgcgg ccagtttttg cagccaacgt acttcaactt gtacacggaa tttcagcaaa
     4741 ccatattcgc tgaaaatccc gcgcagcgcg ctgagtttat cgccgtagcg tccatcgaca
     4801 ggggaaacgg cggtcagtga ggataattcc atagatcaca actccggggt taaatgagca
     4861 agaatttgtt ttgcctgagt ggtcaggcga ttacgagaaa acatcagttg tagacgtccg
     4921 ccgccgacct ggtgccagag cacggcggcg cgaatgcctg ccagcagggt tgcgcgaact
     4981 ttcgcctgca cttgtgggct ttgcagtaca gcaggggaac cggtgacctg aatgcgcggg
     5041 ccaagcgggc tgatcacatc aacatagata gcagccatcg cgctcatcag cgtttcggac
     5101 tgtaaatcga agtgttcgag ctggcgttgc aggccgttga tccggttgcc cagagtgtcg
     5161 agcgcgcctt tcgctgagga gagtttgcgc tcaagcacca tcaagctgag tgtgtagcgg
     5221 gttaattcgg cgtttaagcc ctggcaactg ctggcattga gcacgccgag cagggtttcc
     5281 agcccgacgc gcaggttggc ttcgctaccg ccaaaaaccg ccagcgtcga gctggggttc
     5341 atatcaataa tactgttgag tgagacgtgt agcgcatcgg catcacaatg cccctggtga
     5401 gcgagttgtt gcaccaggcg tgccgactga caaataccgg ccagggcgag ggtgatgtca
     5461 tagtaattct ttgccacgtt cactgcttcc ttgttttaag taaagataat aatcagaccg
     5521 gcagcggcag acgctgctca ataataccgc caccgaggca cacttcaccg ttatagaaga
     5581 cggcagactg gcccggcgtc acggcggcaa ccggctcatc gaaaatcact ttaatgcgat
     5641 cgtcgtccag cgccttgacg gtgcacggaa tgtcggtctg gcgatagcgg gtttttaccg
     5701 tgcaacgcat agtgccggtg aatggttcgc gatcgaccca gtgcagctgc tgggcaatca
     5761 acccgacaga catcagccgc gggtgttcat ggccctgagc gacaaccaga atgttgtttt
     5821 cgacgtcttt gtccaccaca taccacggtt cttcggtgcc ttctttggtg ccaccgatac
     5881 ccagaccttt acgctgaccg agagtgtgat acatcagccc ctggtgctca ccaatttcat
     5941 ggccatcgac ggtaatgatt ttgcccggtt gcgccgggag ataacggccc aggaactcgc
     6001 ggaatttacg ttcgccaatg aagcaaatgc cggtagagtc ttttttcttc gcggtgacca
     6061 gaccaagatc ttcagcaatc ttacgcacct gcggtttttc cagttcgccg accgggaaca
     6121 ggctttgcgc aatctgctca tggctgagcg tataaaggaa gtagctctgg tctttattgc
     6181 tgtccagacc acgcagcagg cggctcttgc catcgacatc ggcccgacgt acgtaatgac
     6241 cggtagcgat ataatcggca cctaaatctt cggcggcaaa ttcgaggaag gctttaaatt
     6301 tgatctcctt gttgcacaga atatccggat tcggcgtgcg accggcttta tattcggcaa
     6361 ggaacagttc gaagacgttg tcccagtact cggcagcaaa gttaacggtg tgcagttcaa
     6421 tgccgagctt gtcgcagacc gcctgggcat cagccagatc cgctgccgct gtgcaatatt
     6481 cctcaccgtc gtcttcttcc cagttcttca taaacaggcc ttcgacctga tatccctgtt
     6541 gttgcaacag ccaagcagaa acggaggaat cgacaccgcc ggacatgccg acgattactt
     6601 tttttgcggt ttcagacatt ggatcactca cgacattgaa cttcaaggcg gcgtattcta
     6661 tcacgcagcc ttttagatga caccctttgt aaaaggccag ttaaaatcgc caatcatctc
     6721 cagcggataa cgttgcccgc tttgataaca acgaatactt tccgccacca gcggcgaacg
     6781 aagatttgac gcctgtaaaa tttcttcggc gctgacccaa cggcagcagt cgatatcgct
     6841 gtcatgaggc tgggtcgggc atatttgctc aagctcaatg gcaaagagga aacgcaaaaa
     6901 cggcgtttta tctggcgcaa tccactgatg catacgaata aagtgttgcg gctgcgcgct
     6961 gatgccggtt tcttcccaca gttcacgggc ggcggcttcc actaaggttt catcggcttc
     7021 cagatgcccg gcaggttggt tccataacgc tttaccatta atcgtctctt caacgactaa
     7081 aaatttgcct tctgcgtgca ccacgcaagc aacggtaacg tgcggtttaa acattttgca
     7141 tccttaatct gtcacttctc gccattcgcc attggcaaga ttatccaaag agtaatctcc
     7201 catcgcatag cgaatcagtc gcagcgtggg gaaaccaaca tgggcggtca tgcggcgcac
     7261 ctggcgatta cgtccttcat ataaggtgat cttcagccag ctggtgggaa tacttttgcg
     7321 ttcacgaatt ggtggattcc gcggccataa ccacgcgggt tcgtcaacca gctccgcgcc
     7381 tgcgggcagg gtagggccat catttaaggt tacgccattg cgcaaggctt caagtgcgtc
     7441 ttgtgtggga ataccttcca cctgcacata atagattttt ccggtgcgtt tacccggctg
     7501 ggttaaacgc gcctgcaacg cgccgttatt ggtcagcacc agcaaccctt cgctatcgcg
     7561 gtcaaggcga cctgctgcat aaacgccctg aaccgggatg aattctttta atgttttgcg
     7621 tccggcttca tcggtgaact gcggaagaac atcgtagggt ttattgaaga ggatcacacg
     7681 cgtgggctgg ttttcatgtt tacgcctggt agaacgttgc gagctgaatc gcttaacctg
     7741 gtgatttcta aaagaagttt tttgcatggt attttcagag attatgaatt gccgcattat
     7801 agcctaataa cgcgcatctt tcatgacggc aaacaatagg gtagtattga caagtcaatt
     7861 acaaatcatt aacaaaaaat tgctctaaag catccgtatc gcaggacgca aacgcatatg
     7921 caacgtggtg gcagacgagc aaaccagtag cgctcgaagg agaggtgaat ggaaagtaaa
     7981 gtagttgttc cggcacaagg caagaagatc accctgcaaa acggcaaact caacgttcct
     8041 gaaaatccga ttatccctta cattgaaggt gatggaatcg gtgtagatgt aaccccagcc
     8101 atgctgaaag tggtcgacgc tgcagtcgag aaagcctata aaggcgagcg taaaatctcc
     8161 tggatggaaa tttacaccgg tgaaaaatcc acacaggttt atggtcagga tgtctggctg
     8221 cctgctgaaa cccttgatct gattcgtgaa tatcgagttg ccattaaagg cccgctgacc
     8281 actccggttg gtggcggtat tcgttctctc aacgttgccc tgcgtcagga actggatctc
     8341 tacatctgcc tgcgtccggt acgttactat cagggcactc caagcccggt taaacaccct
     8401 gaactgaccg atatggttat cttccgtgaa aactcggaag acatttatgc gggtatcgaa
     8461 tggaaagctg actctgccga cgccgagaaa gtgattaaat tcctgcgtga agagatgggc
     8521 gtgaagaaaa ttcgcttccc ggaacattgc ggtatcggta ttaagccgtg ttctgaagaa
     8581 ggcaccaaac gtctggttcg tgcagcgatc gaatacgcaa ttgctaacga tcgtgactct
     8641 gtgaccctgg tgcacaaagg caacatcatg aagttcaccg aaggcgcgtt taaagactgg
     8701 ggctaccagc tggcgcgtga agaatttggc ggtgaactga tcgacggcgg cccgtggctg
     8761 aaagttaaaa acccgaatac cggcaaagag atcgtcataa aagacgtgat tgctgatgca
     8821 ttcctgcaac agatcctgct gcgtccggct gaatatgatg ttatcgcctg tatgaacctg
     8881 aacggtgact acatttctga cgctctggca gcgcaggttg gcggtatcgg tatcgcccct
     8941 ggagcaaaca tcggtgacga atgcgccctg tttgaagcca cccacggtac tgcgccgaaa
     9001 tacgccggtc aggacaaagt aaaccctggc tctattattc tctccgctga gatgatgtta
     9061 cgccatatgg gctggactga agccgcagac ctgattgtta aaggtatgga aggcgcgatc
     9121 aacgccaaga ccgtaaccta tgacttcgag cgtctgatgg aaggcgctaa actgctgaaa
     9181 tgttcagagt ttggtgatgc gatcatcaag aatatgtaat atgtaatcac tacatgtgtt
     9241 aaatatttaa acgggcgtat aacacgcccg ttgttttatt tatgtggata ttattaatag
     9301 catatcgagc atatttatat aaaacctatt acttgagccc atatgggcat atttttataa
     9361 tacaactctt atgcaaacat ttatttgtta tttcgctttc tcctggagga cactcttgac
     9421 tgcttttgag taaactccat aaattcttgt tgaatggtgc aatgttataa atagtaatag
     9481 gatattcttt ttccttaagg ataataccaa gcttaaccgg tgtaaatatc cttccaggag
     9541 gaagaaatat agtagattga taccagatga tcattttcat atcaccccat atggctgaaa
     9601 aagatatgcc gcatgtaggt tgaattaccg tgtcaattac tacccacttc atttgttatg
     9661 tcttatccca cggtatttaa tatagttcat ttggatgttc atttctttat tttgcatatg
     9721 agtatattac cccttcaaaa aataaattaa ttaaaacgat tgcttatata aaacaaaatt
     9781 taaatcaagg aatctcaatg gatgttaaac aaaatgagat ttagtgaaaa caataaatta
     9841 ttcacttcgt tttagatttg tttagctata atgttataca ttcaaatgac tgaacatcct
     9901 gtaattaaaa catagccttt atgctacttg gtgccaattt gctaaacatt atggttgcct
     9961 ttttatataa cgataataat gaatataaac atgacatgag aataaggttt caatttttga
    10021 gttatatagg aaaggtttaa cctgttcctg gctaaaatac atataactgg atgatgacta
    10081 aaccaaaaca catatgcgtt aagtattgaa acgggcgtat ggcacacccg ttgtttttat
    10141 aaatatatta accgttataa aataacgtat aaaaagtcaa gtgatcacat ttcaaatatc
    10201 aatttgatag tatgggcttg gtgattattt atgggtagca ataaaaggac agtatttatc
    10261 atccataggg atagtctctg tacttttatt cccattatgc taatgcctta ctgaattatg
    10321 aagcatttct taagcatcca actttagcta gattaatggt ttattatttt ctacatcttc
    10381 aatatataaa agcgtattat caatggcgta gtaactgcgt ttgttatgat taacatcagt
    10441 aacccaacgg aaaacgcccg cgcctgctag tgttgaacag tattcccgaa atgtagattt
    10501 tccgcaaata tgaagcaatg cggcctcttt tattttagca ggattcttgg tcgtactaac
    10561 ttttaactgg ttcctggttc ctcttaataa caaaaccgtg tcatcgtgag taataattct
    10621 gatgttatcc gtagccagat aataaatgta atgtgcaata cggtgatgtt ttaattctga
    10681 ataaaaccag gagaagtttt gctcttttct cacttgctca aacatctttt gaaaaacaac
    10741 gacctgatcc atcaggataa taacctcttg ttagttgtga gactgcgtag tgtgcacgat
    10801 cggttttacc acttcaatct ggtctgtcct ttggctgtga tatgtacaga gtgtgataga
    10861 gggattacct gaattctccc ggtgagcatt ttgcaacgga ccagctccgg tacaaatgct
    10921 gttgtgggtt cagattataa cattctgtct aaggggcggg ataaaggtga aattaggggg
    10981 catgaaagat gactttataa ccttgctcac cccaatgttg taaaagttcg ttttgccttc
    11041 tcgttggtgc catgcctgtc cagacaatca atgtttgcgt cgggaacagt tccgggcgcg
    11101 gcgattcaat gggatcagca agaacagaaa tgtgccatcc tgacaatgat aaacgccatg
    11161 cttccagcca caatcgtgct ctctcttcaa cattccacgc catcagaatg gcttctttac
    11221 cgggcttacg gcgcgtttcg aaaagcgagg ttgctgcgta ttcaattaat gcgccgtcaa
    11281 acatactgct cataatgcgg gaggtgttgt gatcaagcac gagacgctgg cgaacaggaa
    11341 ggtaaacatg attaatcaat tgatctactg ggtactctcg acccagtgaa ataattctcg
    11401 cgcgtagttt ggcaggatta gccatacgaa gaattgacat catctcttct tgcaggcggc
    11461 tccagtcatc ttccgtatcc tggctggtgg tttccagtaa tgctttaact ttgcctacag
    11521 ggacgccatt actgatccaa cgcttgatct cttcgatgcg ttgtatgtct tcttcatcaa
    11581 agagtcggtg tccgccttca ctgcgctgtg gttttaacaa accgtagtgg cgttgccagg
    11641 cccggagagt gacaggatta atcccgcaac gttcagcaac atcaccaatg ctgtaataag
    11701 ccacaattcc tccttgcggt cacaaatctc cgtcgccggt acacgaccca ataatacttt
    11761 gtacaatata cgctaaaatt gtacaaagta taaataagat ttcagctaaa ttggatgaaa
    11821 cattattttt aatgtagatt aaatttaaac ataacgtatt cattttcacg atgatttact
    11881 gaaatcatgt gaaagaatgt gctgaaaatt attttttctc tggccacgct atggaaggga
    11941 taccattcaa tttagcttta gcaaacagat ctccctgaaa catctcaatt cctgcggatt
    12001 caagccacat ccactcttct ggtgttgcca cgcccatagc actgacttga atttcaagtg
    12061 atgtacagca ttttatgatc gcttgaataa ttgcctgccg tggcccgctt ttatgaacat
    12121 tggtaatcaa ttcctgactg attttaattc tgtcaggctg gaagcgtgac aggagtaaca
    12181 aaccggcaaa acctgcgcca aaatgatcaa ttgctacact gataccggca gccttaagcg
    12241 atttaatcgc ttctgcaaac tcatcaaacc gagatataac ttcactttca gtaaattcaa
    12301 cgatgatttg ttcaggcacc agagcattgg cctttatttc atcaagtaaa aaagagactg
    12361 cgtcaggttc gttaaccagg gtcataggta atagattgat tgaaatcatt ttatcaccga
    12421 gctcaagtgc gtgtgtcatc gcgaatgcaa gcgccttact tttgagatcc gctgtgtaga
    12481 tttccccgtc tttacgctgc ccaaccgcta tggctgatgg gctatcttca tttttttgca
    12541 caatggcttc aaaagcgatt atccgccgcg ataagggatc gacaatagga tgaaaggcaa
    12601 agtggtcgtt tatagttggt gaaagggtac aggaatcaag ttctttatca gatccgtcag
    12661 cgataaaaag ccaggagtct tcggcaggga tctcgaaata ggttgattgt tcggttgcaa
    12721 ggacaaaagt acgaaaaaat tgtagcgctc tgtcatcata agttagctga aattttgatg
    12781 tgcctttgtc gaatacggcc tgtaaaacgt catctcgctc gtgcaggcgc aaatcaaata
    12841 attccattcc cgctttgcca aaacggcgag caggcgcgta atcgcacagc agctcaacaa
    12901 tattatagtg ccgtggatct tggcatatag cccgatatat cattttaacc tgttctttcg
    12961 gaccttccag aagctggaaa aaatgagaac cattaaacag taagatccct gttacgtcag
    13021 actgcatgtt cctgcgattt gctatcgaaa ccatttcttc gatttttttg acaggttcgt
    13081 cgtcacgtat atggctacga taaataaggg tggtaagcat taacaatcca gggtaatggg
    13141 tgaggcgaga gtaagacggt aacagacata tcttcttgtg tctttctttt aataccaaaa
    13201 cataaccgtt tctttacatt gataaaaaat ggaaaaagtt gaacacgagt tggcgaaaaa
    13261 tcttgtatag attgtcagtt aaatgatgca atatgtttta tcataacaca ttgttttata
    13321 tacattagta ctaattgcaa aaaattaatt tatcattctg tacacatatt tcgtacaagt
    13381 ttgctattgt tacctcactt cacattgatt aacattttta acagaggcgt agcatgcatc
    13441 aaaattcagt gacttcagat tctgcgggag caatcactcg ttactttgca aaagctaact
    13501 tgcctactca gcaggaaact ctcggggaaa ttgtgactga gattttgaaa gatggacgta
    13561 atctgagtcg aaagtcgcat tgtgccaaac ttctttgccg actggaacaa gcgacagggg
    13621 aagaggaaca gaaacattat aatgcactaa ttgggttgct ttttgaataa tgcaacagcc
    13681 tgattattta acaggttagc tatgaagtcg ttatgaagac atctgataat gaacgtataa
    13741 aatatgaaat tactggccag gcggtgctcc agatactgcg catgaagata aatttttcat
    13801 tgcagactct tattaagcaa ttactcgtaa tgaaatcggc tgaggaagat gctttccgac
    13861 gcgatttaat tgacagcata attcgtgatt ttagcaatag tgattcagga gggccaaacc
    13921 gaagaacagc gacagctgac aataaaagta tgttcaatgg taagaaaata aacagaatac
    13981 attaaaattt cataagtaag atgagaggtt accatgcttg aagatactac aattcataat
    14041 gcaataactg ataaagcgtt agcaagttac tttcgcagtt cgggtaattt gttagaagaa
    14101 gagtcagcag tgttagggca ggctgtcacc aatttaatgc tttcaggcga taatgttaat
    14161 aataaaaata ttatcttaag tctgatacac tccctggaaa caacaagtga tattctcaaa
    14221 gctgatgtga ttagaaaaac actggaaatc gtgttgcgat acacagctga tgatatgtaa
    14281 cttctaatta ttaagtataa gtttatagaa aactcattca tcttattttt gtctgtcgct
    14341 ttagactata cacagcataa ttttattggg ttaatatttc tacgaggctg acatgaataa
    14401 ttcaatccca gagagattta tttttcaatg tgctttgttt aaaaatctcg agagggaggt
    14461 gttcatgacg catgggtatg ttgatagtca tattattgat caggctttac gcttgcgttt
    14521 aaaagatgaa accagtgtga ttctttctga tctctatctg caaatattgc agtacattga
    14581 aatgcataag actacactaa cggatatcat tattaatgac agggaatccg tgctctctta
    14641 gaacttgagt aaggttttag aataaacatc agctgtatca ccatgctgat gcaaagtgag
    14701 catttcaggc gttatgcttt cttattatgt ccgcaatatc aggtgtcaag aatggagagt
    14761 tctcgctctc cattcttgac gcctgatatc ccgcctaact tattttgtac tgcctacatt
    14821 atgctcaata attaatgggt tagatcaaga tggagaaaat tttatcttcg gcgtggatat
    14881 ttcttatgct ctctaccatg catctctgta gatgccagcg cgagcgaatg aatgg
//
LOCUS       DADLTI010000104        14925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLTI010000104 DADLTI010000000
VERSION     DADLTI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.104"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            597..794
                     /locus_tag="JN755_002817"
     CDS             597..794
                     /locus_tag="JN755_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381560.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JN755_002818"
     CDS             complement(744..1937)
                     /locus_tag="JN755_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381561.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JN755_002819"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JN755_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7381562.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JN755_002820"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JN755_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7381563.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JN755_002821"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JN755_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7381564.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="JN755_002822"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="JN755_002822"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7381565.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="JN755_002823"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="JN755_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7381566.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="JN755_002824"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="JN755_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7381567.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3554 c         3707 g         3644 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADLTI010000105        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLTI010000105 DADLTI010000000
VERSION     DADLTI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.105"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JN755_002825"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JN755_002825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7381568.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JN755_002826"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JN755_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7381569.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JN755_002827"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JN755_002827"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7381570.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JN755_002828"
     CDS             complement(3965..5611)
                     /locus_tag="JN755_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7381571.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JN755_002829"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JN755_002829"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7381572.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JN755_002830"
     CDS             7086..8594
                     /locus_tag="JN755_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7381573.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JN755_002831"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JN755_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY7381574.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JN755_002832"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JN755_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JN755_002833"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JN755_002833"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7381575.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JN755_002834"
     CDS             complement(13693..14283)
                     /locus_tag="JN755_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7381576.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JN755_002835"
     CDS             complement(14512..>14685)
                     /locus_tag="JN755_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7381577.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3819 c         3495 g         3686 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgtagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagggata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacaaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgctggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc actaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatca aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcag gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADLTI010000106        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLTI010000106 DADLTI010000000
VERSION     DADLTI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.106"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            156..1196
                     /locus_tag="JN755_002836"
     CDS             156..1196
                     /locus_tag="JN755_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7381578.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JN755_002837"
     CDS             complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JN755_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7381579.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JN755_002838"
     CDS             complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JN755_002838"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7381580.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGGILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4963..5994
                     /gene="galR"
                     /locus_tag="JN755_002839"
     CDS             4963..5994
                     /gene="galR"
                     /locus_tag="JN755_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7381581.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JN755_002840"
     CDS             complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JN755_002840"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7381582.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7385..8320
                     /gene="lysR"
                     /locus_tag="JN755_002841"
     CDS             7385..8320
                     /gene="lysR"
                     /locus_tag="JN755_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7381583.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JN755_002842"
     CDS             complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JN755_002842"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7381584.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9128..10516)
                     /locus_tag="JN755_002843"
                     /pseudo
     CDS             complement(9128..10516)
                     /locus_tag="JN755_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JN755_002844"
     CDS             complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JN755_002844"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7381585.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JN755_002845"
     CDS             complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JN755_002845"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7381586.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12778..13926)
                     /locus_tag="JN755_002846"
     CDS             complement(12778..13926)
                     /locus_tag="JN755_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY7381587.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14181..14339
                     /locus_tag="JN755_002847"
     CDS             14181..14339
                     /locus_tag="JN755_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY7381588.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
BASE COUNT         3804 a         3980 c         3470 g         3431 t
ORIGIN      
        1 gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca
       61 gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat
      121 attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca
      181 gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg
      241 aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg
      301 ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg
      361 tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga
      421 gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga
      481 atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt
      541 atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga
      601 cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac
      661 gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct
      721 acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca
      781 gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg
      841 aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg
      901 caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc
      961 aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg
     1021 ctcagatggc gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg
     1081 caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag
     1141 acgtattagc tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat
     1201 taacgccggt taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg
     1261 ttattgccag cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta
     1321 ccattaccgc cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt
     1381 tttgtactgc aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg
     1441 cattgagcgg aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg
     1501 ctggcagcag ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg
     1561 ctggcataca gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa
     1621 ccacgatacc aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc
     1681 ccagcgccac cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga
     1741 ataaactggt gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg
     1801 cattcaggaa actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg
     1861 ccagtttggg aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg
     1921 ccagggcgac caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa
     1981 tcgccgctat ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg
     2041 ttaattcgcc gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca
     2101 gcgtatagcc gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca
     2161 ggccgttggc aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa
     2221 acggcgccaa aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact
     2281 ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa
     2341 acgcagagag aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt
     2401 tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg
     2461 acaaagtcag gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc
     2521 ggtacagcaa gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc
     2581 agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc
     2641 gcagtggcat ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc
     2701 gacaccattt cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag
     2761 ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg
     2821 cgaacattct cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg
     2881 ttcattatgt tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac
     2941 aacaggcgtg catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc
     3001 ggtacgttga tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc
     3061 aggccaaatt tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc
     3121 accacatagc gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg
     3181 aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg
     3241 cggtaatgca gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac
     3301 aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg
     3361 gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta
     3421 tggacgacgc cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct
     3481 tccggctgct gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact
     3541 ttgtcggcag tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta
     3601 agttgctccg gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg
     3661 gttttgattt cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc
     3721 atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct
     3781 gcattgggca gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcct
     3841 ccaacaaaca gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct
     3901 tcgacacatt tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga
     3961 gtttcacgcg ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca
     4021 gcgattttgc gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc
     4081 aaaatatgca gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg
     4141 aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc
     4201 gcgacaaaac ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct
     4261 tcagggaaaa tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc
     4321 ataggttgcg tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac
     4381 cactgttggc ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca
     4441 agcaaaatgc catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc
     4501 agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa
     4561 aagctaaaaa gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat
     4621 tacacgagat aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc
     4681 gcgcaggttg gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat
     4741 acctctggga tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac
     4801 agcgtttcgc aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg
     4861 tttggctcac attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt
     4921 aacactgaaa caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat
     4981 gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc
     5041 aaagccagcg aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac
     5101 ccgaacgcca acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt
     5161 ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat
     5221 cacaccggta atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag
     5281 gctattgagc aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc
     5341 ccggatgccg atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt
     5401 atcctgcctg gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg
     5461 gcaacgcgtc atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac
     5521 tctatttctg acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt
     5581 attccggcca atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag
     5641 gcaatgaccg agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat
     5701 tcaatggcgg cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag
     5761 atttcgttaa ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc
     5821 accgtgcgtt acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg
     5881 gcggataatc gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt
     5941 cattcagtgt caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag
     6001 ttaaagcaat tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc
     6061 ctgaccatta tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat
     6121 tgatgcgcca tatgcccctg tatcatgcaa taccagataa tcacctgcct tcacttccgg
     6181 caaggcgcgg gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca
     6241 taacggcccg gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc
     6301 tgccagggca ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa
     6361 cccggcatca accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt
     6421 aattaatacg ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg
     6481 gtggcccaaa tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc
     6541 ggtatcaacc gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat
     6601 agcctgtaaa tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc
     6661 cagatgggca taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg
     6721 atgacgttgt atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct
     6781 gttttcgcca ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg
     6841 cagccatacc cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc
     6901 cgcattcacc ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc
     6961 tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg
     7021 ctctatttcg cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa
     7081 aatatgaata ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc
     7141 tgctatctgc cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc
     7201 gggcaaacgc agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg
     7261 cataacaaac tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa
     7321 aatatcgatt tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc
     7381 gctaatggcc gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg
     7441 aagcctgact gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact
     7501 ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca
     7561 tcctaccgtg caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga
     7621 tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc
     7681 ctgcctgccg gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg
     7741 ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct
     7801 ctcggcccag cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga
     7861 acgtaccgaa ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc
     7921 ggtaaaaaag gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg
     7981 tactgacagc tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg
     8041 catgatcgta gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg
     8101 tgtttcggtg gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg
     8161 gcggttcagt attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc
     8221 atcagcgctg gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac
     8281 ttctcttgac gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca
     8341 tggatcgacg cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca
     8401 atttctgtgc agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact
     8461 tgcgcataat aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata
     8521 atctgattaa ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt
     8581 tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc
     8641 acacgagtca ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa
     8701 ggcagagtgc aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac
     8761 acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg
     8821 gttttatccc attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt
     8881 agcagcattt gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga
     8941 cgatagtaag gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt
     9001 cctctctcct gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca
     9061 acggccaggt gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc
     9121 cgtgaaatca gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat
     9181 gttccagcgt gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa
     9241 tgttcaacgc agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca
     9301 ggaaggccgc gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac
     9361 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
     9421 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc
     9481 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
     9541 acgccatcac gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa
     9601 agaccgcaat gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct
     9661 gttgttctgt ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga
     9721 tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac
     9781 ggacgttacg gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac
     9841 gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt
     9901 cttccgcctc aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga
     9961 ccaccagaat aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt
    10021 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    10081 caactggtac atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga
    10141 ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac
    10201 atctctacgc tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca
    10261 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10321 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10381 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10441 aaacaaacat attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg
    10501 tattgatagt aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg
    10561 aacaattcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10621 tgccgttatg tgatggatat tccaatttta aaattaagtt gaattattga gattattatt
    10681 aaccacctaa ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg
    10741 attatttcat aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg
    10801 cggcagggtc ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc
    10861 aatggtataa ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat
    10921 caggtcactc ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg
    10981 ttgttcatct gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc
    11041 attaacatta atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac
    11101 tccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11161 cgcgatattg ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg
    11221 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11281 gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11341 accaaattca gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc
    11401 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg
    11461 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11521 ctggcccagc ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc
    11581 acttaaaatc ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg
    11641 gccacatggt ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta
    11701 taagctttgg ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg
    11761 ttatgcatca caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg
    11821 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc
    11881 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt
    11941 tccagtacat ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg
    12001 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
    12061 gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca
    12121 acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga
    12181 ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca
    12241 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
    12301 atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca
    12361 tcggcgacaa ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt
    12421 tttgcgtgcg caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga
    12481 agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata
    12541 tgcccatggg ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga
    12601 ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc
    12661 agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt
    12721 tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta
    12781 gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg
    12841 aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc
    12901 gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc
    12961 ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac
    13021 acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac
    13081 accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt
    13141 ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc
    13201 ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc
    13261 gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat
    13321 taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg
    13381 cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt
    13441 ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag
    13501 actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg
    13561 tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg
    13621 tatcgcctga gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt
    13681 gattgcagaa acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg
    13741 ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac
    13801 gccggtacgt tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc
    13861 cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc
    13921 tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta
    13981 tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca
    14041 gagtcataac tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg
    14101 gcgttaatct cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt
    14161 gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt
    14221 tcgcgctcta cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt
    14281 cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc
    14341 cacataaagc cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa
    14401 cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact
    14461 tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg
    14521 aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg
    14581 ttgtattaat tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg
    14641 gatttttggt attcccattg attgcctatt tcttatttct ttcca
//
LOCUS       DADLTI010000107        13956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLTI010000107 DADLTI010000000
VERSION     DADLTI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.107"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JN755_002848"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JN755_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7381589.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JN755_002849"
     CDS             complement(1604..1777)
                     /locus_tag="JN755_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7381590.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JN755_002850"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JN755_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7381591.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JN755_002851"
     CDS             3415..3744
                     /locus_tag="JN755_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7381592.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JN755_002852"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JN755_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7381593.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JN755_002853"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JN755_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7381594.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JN755_002854"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JN755_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7381595.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JN755_002855"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JN755_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7381596.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JN755_002856"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JN755_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7381597.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JN755_002857"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JN755_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7381598.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JN755_002858"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JN755_002858"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7381599.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3657 a         3455 c         3248 g         3596 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacaactgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgctc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggggtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADLTI010000108        13953 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLTI010000108 DADLTI010000000
VERSION     DADLTI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.108"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..493)
                     /locus_tag="JN755_002859"
     CDS             complement(<1..493)
                     /locus_tag="JN755_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381600.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JN755_002860"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JN755_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7381601.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JN755_002861"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JN755_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7381602.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="JN755_002862"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="JN755_002862"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7381603.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="JN755_002863"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="JN755_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7381604.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="JN755_002864"
     CDS             3628..4149
                     /locus_tag="JN755_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7381605.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JN755_002865"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JN755_002865"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7381606.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="JN755_002866"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="JN755_002866"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7381607.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="JN755_002867"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="JN755_002867"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7381608.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="JN755_002868"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="JN755_002868"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7381609.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="JN755_002869"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="JN755_002869"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7381610.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="JN755_002870"
     CDS             complement(8791..9684)
                     /locus_tag="JN755_002870"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7381611.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="JN755_002871"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="JN755_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11563
                     /gene="allC"
                     /locus_tag="JN755_002872"
     CDS             10331..11563
                     /gene="allC"
                     /locus_tag="JN755_002872"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7381612.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11574..12359
                     /gene="allE"
                     /locus_tag="JN755_002873"
     CDS             11574..12359
                     /gene="allE"
                     /locus_tag="JN755_002873"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7381613.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="JN755_002874"
     CDS             complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="JN755_002874"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY7381614.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13754..>13953)
                     /locus_tag="JN755_002875"
     CDS             complement(13754..>13953)
                     /locus_tag="JN755_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7381615.1"
                     /translation="QITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSNG
                     LLVGILLAVLMDNLIPWERIE"
BASE COUNT         3385 a         3680 c         3498 g         3390 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga acctggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccagc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgagtcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg atcccggtga
    12241 aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat ggcgtagggc
    12301 gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta gagatttaag
    12361 tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt tataccgtgg
    12421 ctttttttgt aacattgcct gatgcgcttc gcttatcagg cctacatgac ctctgcaata
    12481 tatagaattt gcgcgatttt caggaaggat atagtactca tgccgcatcc ggcatgagta
    12541 ctgcgctctt atacgaaata aatcgcatcc gctttaaagg caagagtcag tttttaattc
    12601 cctgacctat tttaatggcg caggcaatat ttcgcgcgct gttgaagaga ttggtttcac
    12661 cgctggcgag cacttcggct aaaggtgcca gacgaggcaa aatgctgaat agcgcgtcaa
    12721 tgccgtactg gtgcaccact tccacgccat cacccaatac accagcaatc ccaatcaccg
    12781 gtacattaaa ctgcttcgcc accgacgcaa cgcccagcgg cgctttaccg cctgccgttt
    12841 gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc cccctgcacc gcctgcgcaa
    12901 gattgaccgc attcaacaca atctcaatgc ccggtttaat atccgcattg agaaataccg
    12961 ccgccgcaat ccccataccg cccgcagcgc cgccgcccgc catctgacag acattaattt
    13021 cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc ctgttccagc tcttcaacca
    13081 tctccggcgt tgcccctttt tgcgggccaa ataccgccgc cgcgccgcgt gcccctacca
    13141 gcgggttatc aacgtcacat gccacttcaa tatggctgtt cgccagacgc ggatcgcatt
    13201 cctccatctc aatgcttgcc acgcgcgcta aattgccgcc gttagccgcc agcacctggc
    13261 cgtcggcatc aaggaaacgc acaccaagcg cctgcgccat gcccataccg ccgtcgaccg
    13321 tcgcactgcc gccaatgccg agaataatat ggcgaatgcc gttatccagc gcatggcgaa
    13381 ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt acgcttttca ggcgcgacca
    13441 gcatcaggcc acttgctgcc gccatctcaa tcaccgctgt tttcccgtct ccggtaaggc
    13501 cataaaaagc attcactttt tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc
    13561 cacccgtcgc ggcgaccatc gcatccaccg tcccttcgcc gccatccgct atcggcaaac
    13621 agatatagtg cgcatcggga aagagggtcg aaaacccggc tttaattgcc tgacaacatt
    13681 tttctgcact taagctctcc ttaaaagagt ctggcgcaat gacaatcttc attatcaatc
    13741 ctttttcaac aaattattcg atgcgttccc acggaataag attatccata agaacagcca
    13801 gtaaaattcc gaccagtaag ccgttactga gcagaggacg aagcgttaat ggaaggtctt
    13861 gcaaatacac aggcggtaat gccattaaaa atatgccgac aaataacggc aatgcgagtc
    13921 gataaatatt gcgctcggta aacgttattt gct
//
LOCUS       DADLTI010000109        13952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLTI010000109 DADLTI010000000
VERSION     DADLTI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.109"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(88..435)
                     /gene="grxD"
                     /locus_tag="JN755_002876"
     CDS             complement(88..435)
                     /gene="grxD"
                     /locus_tag="JN755_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7381616.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            770..1597
                     /locus_tag="JN755_002877"
     CDS             770..1597
                     /locus_tag="JN755_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7381617.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1725..2306
                     /gene="sodB"
                     /locus_tag="JN755_002878"
     CDS             1725..2306
                     /gene="sodB"
                     /locus_tag="JN755_002878"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7381618.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2452..3621)
                     /locus_tag="JN755_002879"
     CDS             complement(2452..3621)
                     /locus_tag="JN755_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381619.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3787..3876)
                     /gene="ynhF"
                     /locus_tag="JN755_002880"
     CDS             complement(3787..3876)
                     /gene="ynhF"
                     /locus_tag="JN755_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7381620.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4175..5200
                     /gene="purR"
                     /locus_tag="JN755_002881"
     CDS             4175..5200
                     /gene="purR"
                     /locus_tag="JN755_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7381621.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5197..6129)
                     /locus_tag="JN755_002882"
     CDS             complement(5197..6129)
                     /locus_tag="JN755_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7381622.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6242..7453
                     /locus_tag="JN755_002883"
     CDS             6242..7453
                     /locus_tag="JN755_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7381623.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7744..8892
                     /gene="cfa"
                     /locus_tag="JN755_002884"
     CDS             7744..8892
                     /gene="cfa"
                     /locus_tag="JN755_002884"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7381624.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8932..9573)
                     /gene="ribE"
                     /locus_tag="JN755_002885"
     CDS             complement(8932..9573)
                     /gene="ribE"
                     /locus_tag="JN755_002885"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7381625.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9788..11161
                     /gene="mdtK"
                     /locus_tag="JN755_002886"
     CDS             9788..11161
                     /gene="mdtK"
                     /locus_tag="JN755_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7381626.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11202..12458)
                     /locus_tag="JN755_002887"
     CDS             complement(11202..12458)
                     /locus_tag="JN755_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381627.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12766..12842
                     /locus_tag="JN755_002888"
     tRNA            12766..12842
                     /locus_tag="JN755_002888"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12800..12802,aa:Val,seq:gac)
     gene            12847..12923
                     /locus_tag="JN755_002889"
     tRNA            12847..12923
                     /locus_tag="JN755_002889"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12881..12883,aa:Val,seq:gac)
     gene            13031..13336
                     /locus_tag="JN755_002890"
     CDS             13031..13336
                     /locus_tag="JN755_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7381628.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13462..>13952
                     /locus_tag="JN755_002891"
     CDS             13462..>13952
                     /locus_tag="JN755_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAY7381629.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATD"
BASE COUNT         3415 a         3604 c         3314 g         3619 t
ORIGIN      
        1 tccccgccct gaaggacgag gttttacggc gcaccggata aaggactgac ggatgcccgt
       61 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
      121 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
      181 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
      241 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
      301 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
      361 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
      421 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
      481 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
      541 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
      601 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
      661 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
      721 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
      781 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
      841 ggcccatgct tcaaagcaag ccagggagag ttctgctacc gctcatatca ccaaaaaagc
      901 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
      961 gaaaaaagcc gccagtaaaa gtacgaccaa aagcaaaacc gcttcttccg ttaaaaaatc
     1021 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
     1081 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
     1141 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
     1201 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
     1261 cagctcaccg cgtaccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
     1321 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
     1381 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
     1441 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
     1501 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
     1561 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
     1621 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
     1681 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
     1741 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
     1801 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
     1861 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
     1921 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
     1981 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
     2041 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
     2101 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
     2161 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
     2221 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
     2281 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
     2341 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
     2401 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
     2461 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
     2521 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
     2581 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
     2641 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
     2701 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
     2761 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
     2821 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
     2881 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
     2941 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
     3001 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
     3061 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
     3121 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
     3181 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
     3241 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
     3301 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
     3361 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
     3421 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
     3481 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
     3541 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
     3601 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
     3661 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
     3721 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
     3781 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
     3841 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
     3901 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
     3961 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
     4021 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
     4081 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
     4141 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
     4201 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
     4261 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
     4321 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
     4381 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
     4441 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
     4501 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
     4561 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
     4621 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
     4681 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
     4741 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
     4801 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
     4861 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
     4921 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
     4981 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
     5041 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
     5101 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
     5161 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
     5221 ttccggctcc cgcagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
     5281 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
     5341 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
     5401 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
     5461 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
     5521 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
     5581 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
     5641 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
     5701 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
     5761 aagttcaaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
     5821 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
     5881 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
     5941 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
     6001 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacgcgat gcagctcctg
     6061 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
     6121 tgaccacata gtcagcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
     6181 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
     6241 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
     6301 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
     6361 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
     6421 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
     6481 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
     6541 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
     6601 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
     6661 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
     6721 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
     6781 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
     6841 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
     6901 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
     6961 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcac
     7021 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
     7081 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
     7141 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
     7201 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
     7261 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
     7321 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
     7381 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
     7441 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
     7501 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
     7561 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
     7621 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
     7681 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
     7741 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
     7801 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
     7861 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
     7921 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
     7981 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
     8041 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
     8101 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
     8161 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
     8221 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
     8281 ggactggcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
     8341 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
     8401 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
     8461 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
     8521 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
     8581 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
     8641 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
     8701 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
     8761 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
     8821 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
     8881 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
     8941 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
     9001 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
     9061 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
     9121 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
     9181 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
     9241 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
     9301 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
     9361 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
     9421 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
     9481 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
     9541 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
     9601 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
     9661 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
     9721 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
     9781 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
     9841 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
     9901 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
     9961 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    10021 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    10081 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct ccatggaaaa
    10141 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    10201 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    10261 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    10321 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    10381 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    10441 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgat cctgcggtta tgaaacgact
    10501 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    10561 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    10621 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    10681 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcgaaccgg
    10741 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    10801 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    10861 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    10921 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    10981 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    11041 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    11101 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    11161 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    11221 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    11281 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    11341 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    11401 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    11461 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    11521 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    11581 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    11641 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    11701 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgatcccc cacattcacc
    11761 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    11821 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    11881 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    11941 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    12001 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    12061 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgtaggca
    12121 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    12181 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    12241 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    12301 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    12361 aactcgcctt cagtaagttg cgtcgcgcca atgacctcgc cggtcttaac aatttgcaca
    12421 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    12481 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    12541 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    12601 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    12661 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    12721 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    12781 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    12841 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    12901 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    12961 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    13021 gagaatcgct atggcaactt tgttacaatt tcattttgct tttaatggcc catttggcga
    13081 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    13141 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    13201 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    13261 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    13321 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    13381 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    13441 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    13501 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    13561 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    13621 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    13681 caaaagcaag aagccagcca tctccagcgt tatggcgtta aaaaagaaac cttgcacgat
    13741 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    13801 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    13861 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    13921 acgtttgatt tagcggtgat tgccacggat ca
//
LOCUS       DADLTI010000110        13546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLTI010000110 DADLTI010000000
VERSION     DADLTI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.110"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..894)
                     /locus_tag="JN755_002892"
                     /pseudo
     CDS             complement(1..894)
                     /locus_tag="JN755_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1135..1935
                     /locus_tag="JN755_002893"
     CDS             1135..1935
                     /locus_tag="JN755_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7381630.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2013..2783
                     /locus_tag="JN755_002894"
     CDS             2013..2783
                     /locus_tag="JN755_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7381631.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2831..4189)
                     /gene="mltD"
                     /locus_tag="JN755_002895"
     CDS             complement(2831..4189)
                     /gene="mltD"
                     /locus_tag="JN755_002895"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7381632.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4261..5016)
                     /gene="gloB"
                     /locus_tag="JN755_002896"
     CDS             complement(4261..5016)
                     /gene="gloB"
                     /locus_tag="JN755_002896"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7381633.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5050..5772
                     /locus_tag="JN755_002897"
     CDS             5050..5772
                     /locus_tag="JN755_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7381634.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5769..6236)
                     /gene="rnhA"
                     /locus_tag="JN755_002898"
     CDS             complement(5769..6236)
                     /gene="rnhA"
                     /locus_tag="JN755_002898"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7381635.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6301..7032
                     /gene="dnaQ"
                     /locus_tag="JN755_002899"
     CDS             6301..7032
                     /gene="dnaQ"
                     /locus_tag="JN755_002899"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7381636.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7165..7241
                     /locus_tag="JN755_002900"
     tRNA            7165..7241
                     /locus_tag="JN755_002900"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7199..7201,aa:Asp,seq:gtc)
     gene            complement(7372..8418)
                     /locus_tag="JN755_002901"
     CDS             complement(7372..8418)
                     /locus_tag="JN755_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7381637.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8429..9304)
                     /gene="tssG"
                     /locus_tag="JN755_002902"
     CDS             complement(8429..9304)
                     /gene="tssG"
                     /locus_tag="JN755_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY7381638.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNSALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9421..11304)
                     /gene="tssF"
                     /locus_tag="JN755_002903"
     CDS             complement(9421..11304)
                     /gene="tssF"
                     /locus_tag="JN755_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7381639.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11320..11814)
                     /gene="tssE"
                     /locus_tag="JN755_002904"
     CDS             complement(11320..11814)
                     /gene="tssE"
                     /locus_tag="JN755_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7381640.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11811..12641)
                     /locus_tag="JN755_002905"
     CDS             complement(11811..12641)
                     /locus_tag="JN755_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7381641.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12628..13542)
                     /gene="tagK"
                     /locus_tag="JN755_002906"
                     /pseudo
     CDS             complement(12628..13542)
                     /gene="tagK"
                     /locus_tag="JN755_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3522 c         3619 g         3304 t
ORIGIN      
        1 cgggagctgt ttttacatgc tcgctaagga aatcgataaa agcccggatg cgcgtactta
       61 ccgcacggtc gctgtaatag acggcactga atggcatttc cactggcaac actttatctg
      121 ccattaactc caccaattat ccgcgagcga tttctttgtc gatcatgtag tcggacaaac
      181 acgcaatccc gttgccactc aggcaaagct gtttcagtgt ttccccacta ttggatgaca
      241 aaccgtactt cacctcatgt aattgtccat cactacaggc tatcggccag gtattgaggg
      301 aagcgggttc agtgaatccc aggcaaacat gttgctttaa atcgtcgatc gtttctggct
      361 tcccgtagcg ggaaatataa tcgggggagg cgataatttt tcgataactg ttaaataacg
      421 gcctggcacg taagctggag tccgttaacg taccagcgcg tatcgcgaca tccacttttc
      481 tttcgatcaa attaataatc gtttcggagg agactagcga taaagtgact tccggatagc
      541 gttcacggaa aggcttaatt aacggcatca gaaagtgcag caccactgga gttgcggcat
      601 cgatccgtaa cagtccacgc ggtgtattac gcgtctccat aatttctgat tctgccgctg
      661 ccatctcctg caaaattgac tgtacgcgac gaaaataacg ctcgccttct tccgtcaggc
      721 taagttgccg cgtggtccga ttaagcaggc taacgccaag tttcatttcc agctttttca
      781 ccgcccggct taccgctgag tttgcttgcc ctaaaattgt tccgctgccc ggctaaagct
      841 gccgctttcg acgaccgaaa caaaaatggc gagttcttcc gacgtggctt tcatttttgc
      901 tcctgttgca aaatagatga gatattttga atttatttgt cattaaacca tcaggatgcg
      961 tgatatgtca tccgatttaa tgttctcaat aatgagcaaa tttctgaccg gtgtaagcac
     1021 ttgcttacat gacaatatac aattgctcgt tgaaagagtg agctaaaatc cctataacag
     1081 tagaaccctc ccgagtgcgg aagggttgac gtaatagagg tttcaaagtc aaaagtgcga
     1141 aaaaacacct atgccatgcg ctatgttgcc ggacaacctg cggaaaggat cttaccgccg
     1201 gggtcttttg cgagcatcgg ccaggcatta ccacctggag aaccgttaag taccgaagag
     1261 cgtattcgga tcctggtgtg gaacatatac aaacagcaac gcgctgaatg gttgtcggta
     1321 ttaaagaact acggcaaaga tgcacatctg gtgttattgc aggaagcgca gacaacgcca
     1381 gagttagtac agtttgcgac cgctaactat cttgccgccg atcaggtgcc cgctttcgtg
     1441 ctgccacaac atccttccgg tgtaatgacc ctttcggcgg cgcatccagt gtattgctgc
     1501 ccgttacgtg aacgagaacc cattttgcgt ctggcgaagt cggcactggt gacggtctat
     1561 ccattacctg acacccgcct gttgatggtg gttaatatac acgccgtcaa cttcagtctg
     1621 ggcgtggatg tctatagtaa gcagttactt cctattggtg atcagatagc tcaccacagc
     1681 ggcccggtca ttatggcggg agatttcaat gcctggagcc gtagaaggat gaacgcgtta
     1741 tatcgctttg cgcgggaaat gtcgctgcgc caggtgcgtt ttaccgatga tcagcgccgt
     1801 cgggcgtttg gccgcccgct cgattttgtt ttctaccgtg gtctgaacgt cagcgaagct
     1861 tctgtattgg ttacgcgcgc ttccgatcac aatccgctac tcgttgaatt cagtcccggc
     1921 aagcctgata aataaggtat gtcaggtctg ccacagggca gaccaacgtt tggcgctgtc
     1981 cttttatact caaacaacaa aaggacagcg caatgacaac acaatcccac catgaccacg
     2041 tagaaaagca gtttagctct caagcctgcg aatatctgac cagtaccgta catgcatccg
     2101 gacgagattt acagcgcctg gcggtgcgtc tggctgatta tcctggtgca agtgtacttg
     2161 atatgggctg cggagcaggg catgccagct ttgttgctgc gcaaaacgtg agcgcggtgg
     2221 tggcgtatga cttatctgcc caaatgctgg atgtcgtggc acaagctgcc gaagcccggc
     2281 aactgaaaaa tatcaccacc cgccagggat atgctgaaag tctgccattt gccgataacg
     2341 catttgatat tgttatcagc cgttattctg cccatcactg gcatgatgtt ggtgcagcac
     2401 tgcgtgaagt gaaccggata ttgaaacctg gcggtaggat gattgtgatg gacgtaatgt
     2461 ctccgggtca tccagtgcgc gacatctggt tacagacggt agaagcatta cgcgatacct
     2521 ctcacgtacg aaactacgcc agcggtgagt ggttgaggtt aatcaatgaa gccaatctga
     2581 tagttgataa tttaattaca gataagttac cgctggaatt ttgttcatgg gtcgcgagaa
     2641 tgcgtacgcc agaagcgtta gtagacgcta ttcgcattta ccaacagagc gcatcgacag
     2701 aggtgagaac gtattttgcc ttgcagaatg acggctcttt caccagtgat atcatcatgg
     2761 tagaagcaca taaagcggca taaataaaaa aggcaccggg ggaatcggta cctttttatt
     2821 atctggtttg tcaggaatct ggcatgttgt tgtttttcac aaacaacgtc agcttgtcgc
     2881 ctggttgcag attcgcagtg tcgctgttcc agcgcatcac atctttgatg ttcacgccgt
     2941 ggcgtttagc aatgcttgaa agcgaatcgc ctttgcgcac acgatacgta atgctatcgc
     3001 tgttgtttgc cagtcgctgt gcgctactgc ctgcaccaat cgtcaaactt tggcctggct
     3061 tcagcttaga tccgcgcagt ttgttccact gctgcaaatc tttggtgctt acgccgagac
     3121 gtgaagcgat gcttgaaagc gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg
     3181 gcgtattgtc ggcaaccagc gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt
     3241 cacgcagttg atctgcatgc ttctttggca ccatcacgta ctgcggccca cttgcgccca
     3301 gcgtggagcc tttcacgcca gcgttgaatg tcttcagctt gctgacggaa atccccgcca
     3361 tatctgcaac cttcgccatt tcaaccgggc tactcaggtg cacacgcgcc agagcacggc
     3421 tttcatcggt cgttggcaga cgtacgccat aacgcttgct gtttttgaga atatcactca
     3481 atgccagcat tttaggcacg tactgcttcg tttcctgcgg caacggtaac gaccagaagt
     3541 ccgtggattt cccacgcgct ttgttcgttt taattgcctt catgacccga ccttcgccgc
     3601 tgttataagc cgctacggtc agaagccagt cgccgtcaaa cattttgttc agacgctgca
     3661 tcatattcag cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca taattgcggg
     3721 tctgtttcaa accataattg cgccccgtgc tcggaatgat ctgccagatg cctgcggcat
     3781 tggcgccaga cgttgcgtga ggatcaaaag cgctctccac tatgggtagt agtaccagtt
     3841 ccataggcat gttacgtttt ttaacttgcc ctgctatcca gtacatatac ggctctgccc
     3901 gtaaagttac atcgtggaga tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa
     3961 tccggtcatt ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac
     4021 catctggcgc gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta aactttgctg
     4081 cttccccttg accagctgca gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct
     4141 ggcaacccac gagcaggaca gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa
     4201 tagttgctta aaagacgacc gatcataacg gcgaacggag ccgatgacaa gaaagtttta
     4261 tcagaaccta tctttctttg accttaacca tgcaaaacgc tcttcaggtt gttgcaataa
     4321 tgtttcttca ttaattacat taattaaatc aatatcttcc gttcttaaaa aaacattaat
     4381 ttgccgctca tttttcagaa ttacgggtag tgttatttga ttttttgccc gtaactcctt
     4441 aactttacga taataatcat ttatggacaa atcgtgcgga aaaatactca aagcaaactt
     4501 catatttgat aacgtatatt catgagcaca acataccaat gtatcgtcag gtaacgcact
     4561 tagcttttta agtgattgat acatttgtaa agctgtccct tcaaacaacc gaccacaccc
     4621 accagaaaac agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac agatatgtcc
     4681 taaagtgtga cccggtgtgg taattacact aaattcatgc cctaaaacga aggcagtttc
     4741 gccatctttg actacctgtg ttgttccctt atcttgtgtc tcttgtggac catacaccac
     4801 aatttgcgga aacttttcca ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg
     4861 ggttagaaat atggcctccg gttgccagtt attggcggta atggcgttta atactggctc
     4921 tgcgtctccg ggatcgacaa tcaggcagcg acctgcctca tcattcaaaa cccagatgta
     4981 attgtcatca aaggcgggaa tactgttaag attcatagat tacctctcag tgtaaaacgg
     5041 aaggttgtga tgaaaccggc aagagtccct caaactgtcg tggctcctga ttgctggggc
     5101 gatttgccct ggggagagct ttatcgcaag gcgctggagc gccagctcaa cccgtggttc
     5161 actaaaatgt atggttttca tctgcttaag attggcaatt taagcgcaga aatcaattgc
     5221 gaagcgtgcg cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg
     5281 caggcggatc cacttcatct tccttttgcc gataaatccg ttgatatttg tctactggca
     5341 cataccttgc cgtggtgcac cgatccgcat cgtttattac gtgaagccga tcgggtactg
     5401 attgatgatg gctggctggt gattagtggc ttcaacccca tcagtttaat gggattacgc
     5461 aaacttgtgc cggtattgcg caaaacctcg ccctataata gccggatgtt tactctgatg
     5521 cggcaactgg actggctctc tttgttgaat tttgaagtgc tgcacgccag ccgtttccac
     5581 gttctcccgt ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt
     5641 tgcttacaac ttattgttgc ccggaaacgg actattcctt taacgctaaa tccgatgaaa
     5701 cagagtaaaa acaagccacg aattcgccag gcggttggag ccacccggca atgtcgtaaa
     5761 ccacaggctt aaacttcgac ttggtagccc gtatcttcca gtgtgggatt catcgccgcg
     5821 gcacgagcca gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc tttaacccat
     5881 tcccatttga tttgatgctg ccccaatgca gcatcaagac gttgccagag atcgacattt
     5941 tttactggtt ttttgtctgc tgttttccag ccacgttttt tccagttatg gatccactgg
     6001 gtgataccct ggcggacata ctggctgtcg gtactcaaaa tgacttcgca atgttctttt
     6061 aacgcctcca gcgcgacaat agcggccatc aactccatac ggttgttggt ggtgcgggtg
     6121 tagcctgcgc taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca
     6181 ggtccaggat tacccagaca cgaaccatcg gtgaaaattt ctacctgttt aagcatctct
     6241 ggtagacttc ctgtaattga atcgaactgt aaaacgacaa gtctgacata aatgaccgct
     6301 atgagcactg caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag
     6361 attggtgcac actatgaagg ccacaagatc attgagattg gtgctgttga agtagtgaac
     6421 cgtcgcctga cgggcaataa cttccatgtt tatctcaaac ccgatcggct ggtggatccg
     6481 gaagcctttg gcgtacatgg tattgctgat gaatttttgc tcgataagcc cacgtttgcc
     6541 gaagtagccg atgagtttat ggactatatc cgcggcgcgg agttggtgat ccataacgca
     6601 gcgttcgata taggctttat ggactacgag ttttcgttgc ttaagcgcga tattccgaag
     6661 accaatacct tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt
     6721 aaacgcaaca gcctcgatgc gctatgtgcc cgctacgaaa tagataacag taaacgaacg
     6781 ctgcacgggg cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt
     6841 ggtcaaacgt cgatggcttt tgcgatggaa ggagagacac agcagcaaca aggtgaagca
     6901 acaattcagc gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa
     6961 gagctggcag cacatgaagc ccgtctcgat ctggtgcaga agaaaggcgg aagctgcctc
     7021 tggcgggcat aaatacatgt aaaaggcgct aaaaatagcg acttgggcga tttttgcagc
     7081 aaacgattca aaagatgaga aaaatcgttg acgaaggtcg aggcaatccg taatattcgc
     7141 ctcgttccca acggaacaca acgcggagcg gtagttcagt cggttagaat acctgcctgt
     7201 cacgcagggg gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga aagcccgaat
     7261 cagtaatgat tcgggctttc gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa
     7321 acggatatga tttaaaaaat ggccagacat tataaattct ggctacttct gttactccag
     7381 cgcatcccat ttctccagca gttctactgg aatttcattt attacctccg agacgcgttt
     7441 tccgaccagg cgttctgcct ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg
     7501 ctcaaaccag cgacggatga cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg
     7561 ccggggttct gcgccggtca tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat
     7621 ctgctctgcc tgcatctgtt cagtatgaac gatggtggcg ttttccggct gctcagcctg
     7681 tggttgtggc aggatctccg gcgtggtgct ctgcactgct cccggtaaca gggccagcag
     7741 ttgcgtcaga cgggagaagt ccggtgcctg gtcattcagc gtttcacgcg catggcgttg
     7801 cagccgctca gtggtctctg ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc
     7861 ttccaggtct gccagttgct ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa
     7921 tgcagatagc gaacgctcca catcccgcac ctgaaggcgc atggcagcat tatttgacag
     7981 tgtaatgcca cgaatatccg ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt
     8041 actgcgtgcc accgcagcat cttcggcggt gatatcttca gtcgcgagca gttgaggatg
     8101 gatggcgtcc gggtacgtgg agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc
     8161 cctggcaccg gcctgctgga tacggcagcg cagcaggaca accagaatgc ggatatcttt
     8221 gctgcgcatc agcagacgat gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg
     8281 cgtactgaca aaatcgccat attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag
     8341 caggtattcc ggatcgtact ccagatccgg gccgcagggg cattccgccg aaattgggtc
     8401 agacaggaat atttccattt aattatcctc agtgcgccag gtagtgttct ggttcgaaaa
     8461 tcatgcccgt caccgggttg tcgtgcatgg ctttacccgc ccatgcagtc caacccagtt
     8521 ggtacggacc accggtaaca gccggagggg cggcgtgggg ctttagctgt agctcaattt
     8581 cccagcagta ttcataacca ctaaaggcgc ggaccagttc tgtcaggacg ggcaggttat
     8641 tgctgccagg cagaaaacgt cggtaatcct ccagcgtcag cgggccgata accagccgga
     8701 acttatactg catgtccggc acggcctggc cgatgagtgc actattgcca ataacggaag
     8761 agggggcagg catccccagc cgggtgatct catcgtccgc cacggctatc cagtgcagga
     8821 catactcctg tatccggaaa ggtacaccga aatagtgcgc cagcgtggtg gccagaccgt
     8881 ccggattgcg ggactcacgc accagatggg ccgatgccgc cagacgagcg tgatctggca
     8941 acgggctgtc agcgctctcc cgcaaatcct gcccgctgag gctggcgaca taaaaggcaa
     9001 agcggtcatg ttgcggttta tccagaccgc ccccagcgga ctgggcgctg cgccatgcct
     9061 ggtaaaactg cgtcagccag cggtgatgaa aaagatcaga aaaacgggtc agcgtcggat
     9121 cgcgtcggct ttcagtccgg ttacgcgcca gttcggtgta gtgcagcggc agtggaccgt
     9181 tcggccccca cagcccgagg ctgtacagac tgatgtgcag gcgcccttcc tgccagccca
     9241 cactggcgat ttctcgtggc gcaaaggtca gcgcgggcgt ttgtccgagg cgaaattttt
     9301 ccatccgtgg ttgctaggtg ttgcccgccg gaacggtaca caactgcaca tccacgcggc
     9361 gcatcaggtt cagaaaaccg taatgccagg ggggttcctg cgcctgagtg aaatcgttgc
     9421 tcatacgctt ccccgctgtc ccgccctgac aggccaggtc ataattttgc cgcgctgcat
     9481 gctgtgcagc gtcatctgcg agaaggtatt aatggaaaca tggcgggcga tatagtgttc
     9541 cagcaccagg ccgaacagat acggactgat gccggaaaaa ccttcttcat ccaccgtcag
     9601 ttcacaactg acgccacgcc catagaccag tagtccggaa ccgggcagac ggcgggtgac
     9661 cggggtcgtt ttacaaccaa tcaggctgcg cacctggcgc gattgcggac tgtcatgagc
     9721 aggaataaac aggttcagca gatctcgcaa cgcctggccg ccggtacggt gatccagatc
     9781 ggccagcggc agataattaa aagataactg gcggatcaac cgccatgcca tctcacgttc
     9841 agccagcggg ggctgcggcg agcgtggcgg gcggataagc cctacgcccg ccaccgggat
     9901 ggctgcatcc acggttagat cgtcccggcc attgcgggca ataagacagg gcagatcgcg
     9961 gttagtgacc atggccgtga tggtgatgtg gcgcagattt tccgggtacg gcgcttcata
    10021 ctggtcaacc agcgacagga acacttccga gcccgtatag ggggtacggg tgccgtaacg
    10081 gcgggcgttt tctgataagc ggcggggctc ccggcgcagg gaaaaataac gtccgtgatt
    10141 gccttcatca ttattgcggg tgtggtagag cgggcgaaag gccatcttcc gtgtggtgtc
    10201 ggtttccagc ccttccactt cctggaccga gaacacctca taatccagcg gatgggttct
    10261 gtccaccacc agatgctgtt ccgtcgtgct gtgcgtgaca tcgatccggg cggtgacgcg
    10321 gggaaacagg ttgataaccg gggtacagaa caggctgaac tgtgccgcat ttgtctggtg
    10381 gatcagccag tccggcggca ggcggttcag caggataaca atctccgcca cgccaccgtc
    10441 aattttctgt aatccggcag acaggcctgt cggggtgaaa aaatagaagc gttccggaca
    10501 ggcaaaatat tcatgcagca ggttatgacc gtgaaacacg ttccacgcca gcggcagcag
    10561 cccctgatcc ggctccaggc cttcgtacat aacgggctgt tgcagattca catccagcgc
    10621 gccgtcaaaa tgccccggaa tgccagccag ggtcgcgatg gcgctggtat gtagaagttc
    10681 taacaggtgt gaggccgtac gctcctcgcc gcagagataa aacggcagcc tgtccagtcc
    10741 ggcaagctga ctgaacgtta gttcaccaaa ggtgcgcagg gtaatacgca gcgccccggc
    10801 gacgtgaatg ttggccggca ggtagcggtg cagggcaggc atatccggtg gcgcggcggt
    10861 cagtcgcgcc tcttcgatgg cgagcggcca cagcgttaca tcctggctgc tgcgaaactg
    10921 gcaggcagtg ctttcgcctt ccggaatggc ggagaaaaag gctgtgtcgc gcgggacggt
    10981 aaatcctttc gccagatcgc cttcttccgt atcgggatgc aactgcgcca cgctcattga
    11041 gggcgtgggt gtgacgtaat tggggctgac cacttccagc gggcgctggg tgaaacgggg
    11101 gaattcagcg tcaattttta gctgcgtgcg ggcggtcagg aagctgaagg cctcgaccat
    11161 tcgctctaca tacggatcgg caatatccgt tccctgcatg cccagccggg cggcgacttt
    11221 gggatgacgg gcggcaaatt cggctcccgt ttcccgcaga tagctcagtt cgcggttgta
    11281 gtatcccagt agccgtggat ccatcggtct ctctctgaat taaaaaaacg taatgcgact
    11341 ctgctccata tccagcgcgc tacgtacccg gaatgcagtc ggatacggct gcgtgaggat
    11401 ctcgccgcga atttcaaact gtaatgtgtt atggccactc tggcggcgct catcctgaag
    11461 tgggatgatt tgcagggtgg acgctttcag ccggggttca aaccgggtga tggttcgcaa
    11521 aatggccctg cggatatcgt cccacttgtg ctcatacaga aagctgcccg ccagtggcgg
    11581 caggccgtag ttaagtacgg acgctgccgc gtacggataa cgtgcagcat caatatcatc
    11641 ttcatggctg acggtgttga gcagaaatga caaatcccgg cggatgatct ccttaatctg
    11701 aaccgggctg acgctgatac cgtggtcgcg tttctggttt ggggcgtcgt cacacaggcg
    11761 gtcaaacagc gtgggcagca ggtgattgcc tggacgctgg cgggaagcgc tcacgcgtcg
    11821 cttccttcag cggtgttaaa ggtacaggtg gacaaatcca gcaggctgat atcgccgtga
    11881 ccggtgagcc acaccctctg acccagcgcc tgtaccagtg tctctcccgg accgtcctgc
    11941 cagaccgttt cccggcacag acgcagggag tcggcagcct tttcagagcc gctgtagcgg
    12001 ggaaacagcc aggcactgtg tgtatcgcca ttgttcaggg tgacgttgac gggtttccag
    12061 atcaaatcga tcagatgcgc aggccgcggc gatttcagcg aacagatttg tgaaaacggc
    12121 agccagatat atgctccgcc cgcgatcagt tcgagtaccg gaccggtacg ggagtcgctg
    12181 tcgctaatcc agtcaaatgc gccgccattc cactgcccgg aagtgtctgt tatggcctca
    12241 agcgcgcggc tgcggtgagc atcagcttcc tgcgcttcac cacgggcgtt acaggcgagg
    12301 gtggtcagca gatcctccat ccaggcgggc ggcgggagaa taacaccggg ccgctgttcg
    12361 ccctgaaagc aggcgtggcg gtatatctca cagcggatca gctcgccaaa tacctgcgct
    12421 tcgcgagtat aatttgcgtc catccgggcg catagctgga tctgttgcag tgcccgcgac
    12481 cagtcgcctg ccacgcacaa taactgaaac aggctgtggc ggcgggaggc ctgcgctggg
    12541 tgcatcctga cctgttgctc tgccatctga atgccctcca ccacggaata atcccgcagc
    12601 agcgccgaca aggtaatcgg tagctcgtta ttgtttgtca tgatatatct ctccatgttg
    12661 tgccgggttt atccggtaat ggttgtcaat gccaatgatg cgatgctctt tccgtgtcag
    12721 atctggcagt acggtgttat gcgctgtttc cggggcgtat tccggggaaa gtaaatgcag
    12781 gatatcaggg ggagtctttt cgacaggcca gatagtttca tcttcgccag tggcatccag
    12841 tacattgaga atagcgtcaa tgcttaaccc accagtgacc atatcctgca atgtctcggc
    12901 ttcggtgacc aggtcacgca atgaatccag atcctgcgag gagggctctt cgttaaaagg
    12961 aaaaggatca gcatggcgat taagatcttg ttcgggagat aacaacgcct cgcggtattc
    13021 ctgataaagt cgggtcaggg catttccttt ttccgcacct tctccggtgg aggaggctcc
    13081 atgaacagga atgatatcga atggattttg tgatgtcaga tgctgctgcc tgaaccaatc
    13141 cagatccaga tattcggtta ttgcttccga cgtgatggca gtctgcgata tcgtttttac
    13201 tgtgctcagg tccggagatg atggattgcc atgtacaagg cactatgaag ataaccccca
    13261 ttctatcagg tcgccttcat tcagacgcat acggtgatga ggttccacaa tctggtcgtt
    13321 taccgtacag caatattcgg tagactgatt gataatccac catgcttctt cgtggcgggt
    13381 cagagtgagg gcaatcttta catcatcatt caccttaaaa acaggaatcc agggagaggt
    13441 aacggtgaag ataatttgtg ttccttcagt cgcgtaaatt gtgttatcgg cgatcccgtg
    13501 cgcctgtatt ttttggagtc cccacgtaat atttggtttc attgtg
//
LOCUS       DADLTI010000111        13438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLTI010000111 DADLTI010000000
VERSION     DADLTI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.111"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..352
                     /locus_tag="JN755_002907"
     CDS             <1..352
                     /locus_tag="JN755_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7381642.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JN755_002908"
     CDS             509..1258
                     /locus_tag="JN755_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381643.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JN755_002909"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JN755_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7381644.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /locus_tag="JN755_002910"
     CDS             complement(1900..3237)
                     /locus_tag="JN755_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7381645.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3402..4067
                     /gene="yieH"
                     /locus_tag="JN755_002911"
     CDS             3402..4067
                     /gene="yieH"
                     /locus_tag="JN755_002911"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7381646.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4134..4607
                     /locus_tag="JN755_002912"
     CDS             4134..4607
                     /locus_tag="JN755_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7381647.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4656..5243
                     /locus_tag="JN755_002913"
     CDS             4656..5243
                     /locus_tag="JN755_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381648.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5305..6027)
                     /locus_tag="JN755_002914"
     CDS             complement(5305..6027)
                     /locus_tag="JN755_002914"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7381649.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6029..7204)
                     /locus_tag="JN755_002915"
     CDS             complement(6029..7204)
                     /locus_tag="JN755_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY7381650.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7231..8847)
                     /locus_tag="JN755_002916"
     CDS             complement(7231..8847)
                     /locus_tag="JN755_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY7381651.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8933..10330)
                     /locus_tag="JN755_002917"
     CDS             complement(8933..10330)
                     /locus_tag="JN755_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7381652.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JN755_002918"
     CDS             complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JN755_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY7381653.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JN755_002919"
     CDS             complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JN755_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7381654.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3547 a         3373 c         3080 g         3438 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat ccgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt tattggattt acttatacgg caacgattgc gttgcgcaaa
     3361 tcggctctac gaaaacgtta aactgattaa aaaggaaagg catgtcccgg atagaagcgg
     3421 tatttttcga ctgcgacggt acgctggtcg acagtgaagt catttgctcc cgcgcatatg
     3481 taacgatgtt tcaggaattt ggtattacgc tcgatcctga agaggtattc aaacgtttca
     3541 aaggtgtaaa actgtacgaa attatcgata ttgtttccct tgaacatggt gttacgttag
     3601 cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc tcggctgttc gattcagaac
     3661 tggaagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg
     3721 tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat atgttgcact
     3781 acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca gacccggcgt
     3841 taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt ctggttgatg
     3901 actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc tacttctgcg
     3961 ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc catctttcgc
     4021 agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc ttcacactcc
     4081 cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt gatatgtctg
     4141 tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg ttaattggtg
     4201 ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt ttggtaataa
     4261 tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt cctgcattgt
     4321 taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt
     4381 gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca gttattgtac
     4441 atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt gacagtctcg
     4501 ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc attacccgtc
     4561 tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg ggatacttta
     4621 tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc aggccgttac
     4681 cccaattcaa atatcatccc aagccactgg aaacaggcgc atttgaacag gataaaaccg
     4741 tagagtgcga ttgctgtgaa caacagacgt cagtttatta ctcgggtccc ttttattgcg
     4801 ttgatgaagt tgaacatctc tgtccgttgt gtattgcgga cggttctgct gctgaaaagt
     4861 ttgcaggtag ttttcaggat gatgccagca tagaaggtgt tgaatttgag tatgatgaag
     4921 aggacgaatt tgccggtatt aagaacacat atcctgatga aatgctgaaa gagttggttg
     4981 aacgcacgcc aggttatcat ggatggcaac aggaattctg gcttgcgcat tgtggcgatt
     5041 tctgtgcttt tatcggctat gtgggctgga atgatataaa agatcgcctc gatgaatttg
     5101 ccaaccttga agaagattgt gagaatttcg gtattagaaa ttctgatcta gctaaatgcc
     5161 tgcaaaaggg tggtgattgt cagggttatc tcttccgctg tctccactgc ggcaagctga
     5221 gactgtgggg tgatttttcg tagttattta aataatgaga acaggccgga gcgtaattca
     5281 tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag cggctttatc
     5341 agcgattacc atcagcgaag gatgcagctg caacacggaa gcaggaacat cttcggtaac
     5401 cggaccttgc agcatatttt tcagtgcctg cgctttacct gcgccactga caattatcag
     5461 caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa
     5521 cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc ccaggatcgg
     5581 gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac aaaaatgacc
     5641 atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc
     5701 atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg
     5761 ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt tgccgcgaaa
     5821 gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac ctttaaccag
     5881 ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac
     5941 acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc tcatttcctg
     6001 gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc tgtacgaagg
     6061 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
     6121 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
     6181 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
     6241 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
     6301 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
     6361 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ctaaaacgct
     6421 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagca
     6481 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
     6541 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
     6601 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccagta tcaccaaaac
     6661 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
     6721 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
     6781 caccatgcgc aacagaacgc gtatccagat aactgcctag caccagaatc atactggagt
     6841 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
     6901 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
     6961 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
     7021 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
     7081 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
     7141 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
     7201 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
     7261 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
     7321 tttgatatac gtggcgtaga aacgaatttc cagacgcgag gtcaacatac tggtattgac
     7381 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
     7441 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
     7501 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
     7561 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
     7621 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
     7681 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttcct
     7741 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
     7801 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
     7861 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
     7921 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat gggtattaat
     7981 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
     8041 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
     8101 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
     8161 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
     8221 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
     8281 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
     8341 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
     8401 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
     8461 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
     8521 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
     8581 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
     8641 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
     8701 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gttgttccac
     8761 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
     8821 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
     8881 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
     8941 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
     9001 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
     9061 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
     9121 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
     9181 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt acgcctaatc
     9241 cattctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
     9301 gtaatccaac cggatcaatt tgccaccccc tctctgaact tttcagatgc ggattgggga
     9361 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
     9421 cagtcatgta ataactaaaa gagatgaaat ctacagtatg ttttaaatct tctgcgtcac
     9481 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
     9541 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
     9601 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
     9661 cgattttcgc ttcggggatc aggctatgat aggctttaac tgcccgcgca ctggcaacca
     9721 gttgatgatg gatcgcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
     9781 ccgtgaatgg cgcgtgtaac gacatattga tttcattaaa cgtcagccat aacgctactt
     9841 tatgttggta gcgggtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgccc
     9901 gattagccca accgccgtag tttttcacca gcccatatag catttcgtaa tgggataacg
     9961 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    10021 accccgcttc attcggttcg gcttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    10081 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    10141 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    10201 ttcccattac gccatgaggc tgtaaatctg aggtcgtgat ccctttgcca tcttccagcc
    10261 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    10321 ctttcgtaat taactccttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    10381 ttatctgcgc cgtgccgtgg ggtaatacgt ctgtaaaatc atcgctatta ctgattaata
    10441 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    10501 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    10561 gttttacggt gtcgataccg acatgaatca ggatctccac accatcatct gactcaatgc
    10621 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    10681 caccaaccga gggcagaatg gcgatacctt tacccagcag gccactggca aacgtggtat
    10741 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    10801 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
    10861 tactggtgat gaaatgaagc atcaacgtac caacaaatgc gcaacctatg gcaatgacac
    10921 cgccaataac gctggcccag acggtgaaat caatccccgt tgacgggatg gtctgcatga
    10981 aggtgaaaat acttggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    11041 tggcccccaa tgctccactg atacaggcga taacaaaggg gtacttacgc ggcaggttga
    11101 cgccatatac cgctggttcg gtgataccaa acagacccgt caacgccgct gatcccgcca
    11161 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    11221 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    11281 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    11341 cgcccattac cgcgcccgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    11401 caatcatttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    11461 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    11521 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    11581 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccggc gcatccgctt
    11641 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctcca ccaatcacca
    11701 tggcagtaaa tggattaccg ccgaagcgtt tgcccgcggt gtatcccagg attatcggga
    11761 agaacccaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    11821 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
    11881 tcagaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    11941 tacctttctc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    12001 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tgaccaccgc
    12061 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    12121 catcctttaa cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg
    12181 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    12241 tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    12301 tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag
    12361 tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc gggtgaaatt
    12421 ttacgttgat actgcaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc
    12481 gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt
    12541 tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata actcaagctt
    12601 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaac
    12661 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    12721 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    12781 aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcaac
    12841 gggttgggca gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    12901 agcgagatat aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataaga
    12961 cgatcacagg ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg
    13021 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    13081 ccagcgcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    13141 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    13201 acctttttta taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt
    13261 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
    13321 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
    13381 accatgctcg cagttattaa ctttgtgtaa ttttaggaat ttataaagtt atatataa
//
LOCUS       DADLTI010000112        13314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLTI010000112 DADLTI010000000
VERSION     DADLTI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.112"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..666
                     /gene="phnM"
                     /locus_tag="JN755_002920"
     CDS             <1..666
                     /gene="phnM"
                     /locus_tag="JN755_002920"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7381655.1"
                     /translation="TLVSLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQL
                     ALAARWSQPNRESIAALCRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEA
                     SRKHGMNVLMGAPNIVRSGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADD
                     QSNRFTLPQAVKLVTQNPAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGK
                     RVF"
     gene            666..1223
                     /gene="phnN"
                     /locus_tag="JN755_002921"
     CDS             666..1223
                     /gene="phnN"
                     /locus_tag="JN755_002921"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7381656.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            1210..1644
                     /gene="phnO"
                     /locus_tag="JN755_002922"
     CDS             1210..1644
                     /gene="phnO"
                     /locus_tag="JN755_002922"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7381657.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            1646..2404
                     /gene="phnP"
                     /locus_tag="JN755_002923"
     CDS             1646..2404
                     /gene="phnP"
                     /locus_tag="JN755_002923"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7381658.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(2551..2880)
                     /locus_tag="JN755_002924"
     CDS             complement(2551..2880)
                     /locus_tag="JN755_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381659.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRSCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(2949..3581)
                     /locus_tag="JN755_002925"
     CDS             complement(2949..3581)
                     /locus_tag="JN755_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7381660.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(3662..5877)
                     /locus_tag="JN755_002926"
                     /pseudo
     CDS             complement(3662..5877)
                     /locus_tag="JN755_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            5991..7511
                     /locus_tag="JN755_002927"
     CDS             5991..7511
                     /locus_tag="JN755_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7381661.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            7508..8482
                     /locus_tag="JN755_002928"
     CDS             7508..8482
                     /locus_tag="JN755_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7381662.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            8513..9454
                     /locus_tag="JN755_002929"
     CDS             8513..9454
                     /locus_tag="JN755_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7381663.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            9509..10192
                     /locus_tag="JN755_002930"
     CDS             9509..10192
                     /locus_tag="JN755_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7381664.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            10207..11067
                     /locus_tag="JN755_002931"
     CDS             10207..11067
                     /locus_tag="JN755_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY7381665.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            11064..12065
                     /locus_tag="JN755_002932"
     CDS             11064..12065
                     /locus_tag="JN755_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7381666.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(12062..12768)
                     /locus_tag="JN755_002933"
                     /pseudo
     CDS             complement(12062..12768)
                     /locus_tag="JN755_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            13059..>13314
                     /locus_tag="JN755_002934"
     CDS             13059..>13314
                     /locus_tag="JN755_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381667.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         2953 a         3729 c         3679 g         2953 t
ORIGIN      
        1 acgctggtgt cgctgatgga ccactcgccg ggccagcgcc agttcgccaa ccgcgagaag
       61 tatcgcgaat attatcaggg caaatactcg ctcaccgatg cgcagatgca gcagtacgaa
      121 gaagagcaac tggcgcttgc cgcacgctgg tcgcagccga atcgcgaatc catcgccgcc
      181 ctgtgccgcg cgcgacaaat tgcgctcgct agccatgatg acgccaccca cgcccacgtt
      241 gccgaatccc acctgcttgg cagcgtgatc gccgaatttc ccaccacgtt tgaagcggcg
      301 gaagcctcgc gcaagcatgg catgaacgta ctgatgggcg cgccgaatat cgtgcgcagc
      361 ggttcgcact ccggcaacgt ggcggccagt gaactggcgc agcttggcct gctggatatc
      421 ctctcttccg actactaccc caccagcctg ctggatgcgg catttcgcgt cgccgatgac
      481 cagagcaacc gctttacgct gccgcaggcg gtgaagttag tgacccaaaa tccggcgcag
      541 gcgcttaatc ttcaggatcg cggggtgatt ggcgagggca aacgcgcgga cctggtgctg
      601 gcacatcgca aggacaatca tattcatatc gaccacgtct ggcgtcaggg taaaagggtg
      661 ttctgatgat gggaaaactg atttggttaa tggggccgtc cggctccggg aaagacagcc
      721 tgctggcgga aatccgcctg cgggaacaaa ctcagttact ggtggcgcat cgctacatta
      781 cgcgcgacgc cagcgccgga agtgaaaacc atatcgccct gagcgagcag gagtttttta
      841 cccgcgcggg gcaaaatctg ttggccttaa gctggcacgc taacagtctg tattatggcg
      901 tcggcgttga gattgatctc tggctgcacg ccggattcga cgtgctggtc aacggctcac
      961 gcgcccatct gccgcaggcg ctggcgcgct atcaatcggc gctgctgccc atttgcttac
     1021 tggtttcgcc ggagatcctg cgccagcgcc tcgaaaaccg tggtcgtgaa aatgccagtg
     1081 aaattaacgc ccgactagca cgcgctgccc gctatactcc tcaggattgt cttacgctca
     1141 ataatgacgg cagcctgcgc cagtcggtcg acaagctgct gacgctgatt catcagaagg
     1201 agaaacacca tgcctgcttg tgagcttcgc ccggctacgc agtacgacac cgacgcagtt
     1261 tacgcgctga tttgtgagct aaaacaggcg gagtttgacc accatgcgtt tcgcgtgggc
     1321 tttaacgcca acctgcgcga cccaaacatg cgctaccatc tggcgctgct ggatggcgaa
     1381 gttgtcagca tgatcggcct gcatttgcag tttcatctgc atcatgtcaa ctggatcggc
     1441 gaaattcagg aactggtggt gatgccgcag gcgcgcggtc tgaacgtcgg cagtaaatta
     1501 ctggcatggg cagaagaaga agcccgccag gccggggccg aaatgaccgc gctttcgacc
     1561 aacgtgaaac gccacgacgc gcaccgtttc tatctgcgag aaggctacga gcagagtcac
     1621 ttccgcttca ccaaggtgct gtaacatgag cctgaccctc acgctcaccg gcactggcgg
     1681 cgcacagggc gttccggcat ggggctgcga gtgcgcggcc tgcgaccgag cgcagcgctc
     1741 accgcagtat cgccgccagc catgcagcgg cgtagtgaag tttaacgacg caatcaccct
     1801 gatcgacgcc gggctgcacg atctcgccga tcgctggtcg cccggatcgt tccagcagtt
     1861 tttgctgacg cattatcata tggatcacgc ccaggggctg tttccgctgc gctggggcgt
     1921 gggcgatccg atcccgatct acggcccgcc ggatgaccag ggttgcgacg atctgtttaa
     1981 acatccgggc ctgcttgatt tcagccacac agtggaaccg tttgtggtgt ttgatttgca
     2041 ggggttgcag gtcacgccgc tgccgcttaa ccactcaaaa ctgaccttcg gttatctgct
     2101 ggaaacggca catagccggg tggcgtggct atctgacacc gctggcctgc cggaaaaaac
     2161 gctgaaattt ttacgcaata atcagccaca ggtaatggtg attgattgca gccacccgcc
     2221 gcgcgcggat gcgccgcgta atcactgtga tttaaatacc gtgcttgcgc tgaatcaggt
     2281 tatccgctcg ccacgggtga ttctgaccca tatcagccac cagtttgatg cgtggctgat
     2341 agaaaacgca ctgccgtcgg ggtttgaggc ggggtttgat gggatggaga ttggggtggc
     2401 gtgatgagag ggaatgtgcg cgctggtccc ctcaccctaa ccctctcccc agaggggcga
     2461 ggggagtgat tgtgctcgat attgaatatt gcgctggttt tctccctctc cccattgggg
     2521 tgaggggcga tgcctgctcc atacccaacc tcatcgccca tactcatctt ccattctccg
     2581 ctcttcatct tctagctgcc gacgctcctg atcaagctgg cgctggcgat cgtccagctg
     2641 cctgcggcga tcttcaaact ggcagctgcg gtcgtcatat tgtctgcgcc gatcgtcgct
     2701 cacttcacgc tgccagccgt cgtcgcgcga atcttcatag tctcgcccac ggtcagagtt
     2761 ataagcgtca ttaatcgcct gctgaatatt accaatagta tcgtcgataa tatcggcctg
     2821 ggccggaacg gtggacagcg tgagcagggt gaaaagaaaa agcgtggagt aaggtttcat
     2881 aagccagtct ctgcgatgga ctggcttaac gttagttgag tgtgaatgac cggagtagcg
     2941 gagtaatctc aaattcggta aggcgcagac tcttccatta gctggttagc tcgctgttta
     3001 ttttccgcca ccaatacaat gccatgacat ttttgatccg gtgaaatact ccagatacgc
     3061 tcttccggaa tgcagcagcc atcgtagatc actttcgtgc catcgtaggg ccaaaatgcc
     3121 ccttgttcag tgtctaatac aaacacggaa atctgatcgg actcgcgcgc gatcttctgc
     3181 cagagcatct tcccgccatc atattgatca aaatcgctga ttagcgtgat accgcattgc
     3241 aggattagcg cttcatacag ccgagttgcc agacccttat cgcgctcacc tggagagaca
     3301 caaatacctt ccacctgtac cagatccgag tcttcccacc agcgttcaac ggcaatgata
     3361 tcgctgccga actctttttt acgctgcaga tcgagagcgg caagtacccg atgacgcggc
     3421 acgcctgcaa aaacttcaat gtccgcttca atgatggccg ctcttggcgt ctggttgtca
     3481 taaattacct gtaacagacg tccagaaagc tcgatctctt ccaccacgcg aaaagaacga
     3541 gcaaccgtca tagggctata ggtggttggc atcacgatca tatgtaaatc acttcaattc
     3601 agaacagaat tgctcacaat agcagcacgt ttgcttcgag cctcttgata aattctgaaa
     3661 atcatccctc aatttgcccc agcacctgcg ccagctgcgc gcgggtaaag ggtttgcgca
     3721 gcagcgccac gtccggcagc gcggggttat ggctggggcg taaatcctga ccgctgatga
     3781 gtagcagcgt caggtgcggg taaagtttgc gggccgcatt gaccacttct gcgccgctca
     3841 tgccgccagc aggtccagcg cctgttcgcc gctgctcgct tccagtgtca aatagcccag
     3901 caggtgcaac tgctcgcaga tggtctggcg caccgcggct tcatcttcca gcactaacac
     3961 cagtttatcg ccattaacag ctgtctgttc taccgccgct ggcgataggt ttttcgccgc
     4021 cgtcagcgcg cggggaagct gtaatctgac cgttgtcccc tgccccggcg cgctttcaat
     4081 ttccacgcgc ccgccggact ggcgcacgaa accatagacc atcgacaagc ccagcccgct
     4141 gccgctcccc gtctgtttgg tggtaaagaa cggctcgaac acctggcttt tcaccgcctg
     4201 cgacataccg ctgccatgat cgatgacctc cagtgccacc atgtcctgac gctgcccact
     4261 gctgcgggta acacgctgat tccaggtacg aattttgata attcccgtct gcccttccat
     4321 cgcgtcgcgg gcgttcatca ccaggttgat gatggcgttt tccagttggc tgacgtcaat
     4381 ccacgcggac cacgccggag actgcgcttc aatctcaatc gtcagcgtcg ccggtaagga
     4441 gtggcgcatt aattcgctta actcttccag cagtgttttt agttcaacgg cttgcggatg
     4501 taaggactgc ttgcgggaaa atgccaacaa tcgctgggtc agtagcgcgc cacgttcggc
     4561 ggcttgctgc gcccgctgta ttcgcggcgc gtcgggtgag ttcgtgttca ccagttccag
     4621 gctaccaata attaccgcca gcagattatt aaaatcatgg gccagcccgc ccgtcagttg
     4681 cctgaccgct tttaacttct ggctgtgcaa tagcgcctct tccagcccct gccgctcggt
     4741 ttgggcaatc tccatctgcg tggttttctc tttcaacagc cgtgacgttc gttcaagtga
     4801 tgcggtattg cgggcaaaaa cgttaaaggc gcgcgccagt tcacccaact catcgcgccg
     4861 ctgtaacgcc ggaaaagtga cgtccgcttc gccgtgcgcc aggcggctca tcgcgcggga
     4921 aatcgccgtc aggttagagc cgagattgcg atagatatac caacctgcaa acgcggtgat
     4981 caccaccgct aacagggcga aaatcatgat cagcctctcg ccggagcgca aatcctggta
     5041 actttgctcc acccgctgac cagagagcgt cgccacctgt tcggcgtaac ggttgatgtc
     5101 gccattaagg atcgccacca gcgctttgat gtgaaacata taccagctga ttgccagatc
     5161 gctttgctcc agagccgccg aaagtggcgc gagtttacgc agttcacgat taaaatcagc
     5221 gagtagcgta ttaaccaatg gcgtatcgct gtgagttggc aaggcagaca tcactccgac
     5281 cagttgatga ataatcgcct gcggcgcggg ggtggcaatg gcggcaacaa tcagtcggtt
     5341 catctcttta agcaaaatat tatcttgcgc gtgagtgact ttttgcagat gccgcagata
     5401 acttaagttt tgatacagcg aacttaacag cgcgttacgc tccagatgcc gcctttgccc
     5461 gcgttccagc attccgccga cgctggtttg cagttcgttg ctacgcgtga tgatgcgcgt
     5521 caccagcccg gcttcctgct gcgctaaagg cgcgttcgcc agttgtgcaa gcgagctttt
     5581 taatgcccgc tgtgtctcct gcaaccgctc ggcttcgcct ttatattcca gtgctcccac
     5641 cacctgcgac aatcgcactg ccgccgttgc cacgtttgcc gtgtcgcgcg ccagattcag
     5701 gctgccggtc atatcatcta cagtttgctg cttcaccagc tcttgcatct ggctggcatg
     5761 acgaaagccc attaccgcca cgccgctgac catcaacgtc accgccacca ccagcaaatt
     5821 caaaagcagc aatcgcccgc gcgcgctggc aaaaaagtga cgtccggggt gcgccatatc
     5881 acgctccgtg aagaaaaatc gcaatatgac aaatatgaac ggcttctgac attttgcatt
     5941 tactttcctg tgatggagtg ctgatatcga caacctcgca ggagtccacc atgaacagca
     6001 cgccggttct tgagatgcgc aatattgcca aagccttcgg caaattttat gcgctcaaag
     6061 gggtggattt gacggtctac cctggcgaga tccatgccct gatgggggaa aacggcgcgg
     6121 gaaaaagcac gctgatgaag gtgctggcgg gggcgtatac cgccaccagc ggcgagatcc
     6181 tgatagacgg caagcccttt cacattcgca cgccaaaaga tgccttaagc gccggtatta
     6241 cgctgattta tcaggagatg cagctggcac ccaatttgac ggtggcagaa aatattttcc
     6301 tcggcagcga gctttcccac ggcgggctgg tgcagcgaaa agagatgctg gtgcaggcgc
     6361 aaagagtgat cgaccgcctc ggcgcgcagt ttaacgccag cgataaggtc atgacgctga
     6421 ccattgccga acaacagcag gtcgaaatcg cccgcgcgct acatcgcaac agccgcattc
     6481 tggtgatgga cgaacccacc gctgccctct cctcccgtga aactcaccgt ctttttgaac
     6541 tgattatgcg gctgcgcgat gaagggatgg cgattatcta catcagccac cgtatggcgg
     6601 aagtgtatga gctttccgat cgcgtcagcg tgctgcgcga cgggcaatac gttggcagcc
     6661 tgacccgcga taacctcaat gccggggagc tggtgcggat gatggtcggc aggccactga
     6721 gcgatctgtt caataaagag cgcgatatcc cgctcggtaa agcccgcctg aatgttcacc
     6781 acctgaccga cggcggcaaa gtccagccga gctgcctgca ggtgctttcc ggcgaaattg
     6841 ttggcctcgc cgggctggtg ggagccggac gttccgaact ggcgcagtta attttcggcg
     6901 tgcggaaagc gacaggcgga atgattgaag tcgatggcga accggtggtg atccactccc
     6961 cgcgcgaagc catcgatctt ggcattggtt ttctcaccga aaaccgcaaa gaacaaggct
     7021 tattccttga aatggcggca gtcgaaaaca tcactatggc aaccctggag cgcgatgcca
     7081 gctggggaat gctcaatcgc aaaaaagcgc aaaccatttc cgatgacgcc attaagttgc
     7141 tcaacattcg cgtgcctcat gcccaggtac gcgcgggcgg gctttccggc ggcaatcagc
     7201 aaaaactgtt gatctcccgc tgggtggcga ttggcccgcg cattttactg ctcgatgaac
     7261 ccacccgcgg cgtggacgtt ggcgcaaaaa gtgagatcta ccggatcatg aacgagatgg
     7321 cgcgcaaggg cgtggcgatc ctgatgatct ccagcgaact gccggagata gtgggaatga
     7381 gcgatcgcgt ccatgtgatg cgcgaaggca gcattgccgg tgagctaaat ggcaaaaaca
     7441 tcacccagga aaacattatg accttagcga ctggcgtcaa cgacgcccat tcccaggcgg
     7501 tgacctcatg acgacctctg caaaaacctc tgccaaaaaa atgctgatga gcgatctgat
     7561 gcaaacggtc ggcattttgc ccattttgat cctgatcgta gcggtatttg gctttatcgc
     7621 gccaaacttt tttaccgaaa gcaacctgct aaatattacc cgccaggcgt cgatcaacat
     7681 tgtgctggcg gcggggatga ccttcatcat tttgaccggt gggatcgatc tttctgtagg
     7741 ttcaatcctc ggcaccacgg cggtggcggc aatggttgtc tcccttagcc cggagttcgc
     7801 catgttgtct atcccggcgg cgctgatgct cggcctgctg ctggggctgt tcaacggcgc
     7861 gctggtggcc tttgccggat tgccgccgtt tatcgtcacg cttggcacct atacggcgct
     7921 gcgcggggcg gcgtatctgc tggctgatgg caccacggtt atcaactcca acattaattt
     7981 cgagtggata ggcaataact atctcgggcc ggtcccgtgg ctggtggtga ttgcgctggc
     8041 ggtgatcgtg ctgtgctggt ttattctgcg tcgcaccacg cttggcgttc atatctacgc
     8101 cgtcggtggc aatatgcagg cggcgcgtct taccggcatt aaggtctggc tggtgctgct
     8161 gtttgtttat ggcatgagcg gcctgctttc agggcttggc gggattatga gcacctcgcg
     8221 gctgtacagc gccaacggca acttaggtat gggttacgaa ctggatgcca ttgcggcggt
     8281 gatcctcggc ggcaccagtt ttgtcggcgg catcggcacc atcaccggca cgctggtggg
     8341 ggcgctaatt atcgccacgc tcaacaatgg catgacctta atgggcgtct cttacttctg
     8401 gcagctggtg atcaaagggg cggtgatcat cattgcggtg cttatcgaca aataccgtac
     8461 ccgacatcat caaagtgcat aacaacaata ccctacacat gaggaataca gcatgagatt
     8521 gaagccctta gtgaccgcgc tctgtgcagg cgcgctgctg gccgcagcac cgttcgccca
     8581 ggcgaaagat ctgaaatcga taggcgtaac ggtaggtgac ctggcgaacc cgttcttcgt
     8641 acagatcacc aaaggcgcgg agctggaagc acgcaaactg gcgggcgata acgtcaaagt
     8701 gacgctggtt tccagcggct acgatctggg tcagcaggtc tcgcagatag acaactttat
     8761 cgccgctaac gtcgatatga tcatccttaa cgctgccgat tcaaaaggga tcggcccggc
     8821 ggtgaaacgg gcaaaagacg ccgggatcgt ggtggtggcg gttgacgtgg cggcagaagg
     8881 tgccgatgcc accatcactt ccgataacac ccaagcaggc gaaatggcct gtaaatacat
     8941 caccgatcgc ctgaaaggca aaggcaacgt ggtgatcatc aacggaccgc cggtgtccgc
     9001 cgtgcaaaac cgcgtcgaag gctgccagac cgaatttaaa cgccatcctg acatcaaggt
     9061 gctctctgac aacaagaacg ccaaaggcag tcgtgagggc gggctggagg tgatgaccgg
     9121 gctgctggcg gcaaatccga agatcgacgg cgtatttgcg attaacgatc cgacagcgat
     9181 cggtgccgat ctggcagcaa aacaggcgca acgtaacgag ttctttattg tcggcgtgga
     9241 tggaagcccg gacgccgaag aggcgctcaa gcgtgagaac tcgttgtttg tcgctactcc
     9301 ggcgcaagat ccacaggtga tggcagcgaa agcggttgag atcagttatg acattttgca
     9361 gggcaaacct gcgccgaaag agccggtgct gatcccggtg acgatgatcg ataaaaacaa
     9421 cgtcggcaat tacaaaggct ggacggtgaa ataagtccac acgccgggcg gcgatgctgc
     9481 ccggccaccg ctaaggagaa cgctttcgat gaaacgctcc gctataaaca acattctcgg
     9541 ccatacacgg caattctttt cgcaacatga cgtacatctg ccgccctttg ccagtttctc
     9601 tcctgctcaa tggcagcagc tcgacaccgc cgcgtgggag gtggtttttg atctcaaact
     9661 cggctgggat gttaccgctt ttggtggtaa caactttgca gcacagaggc tgacgctgtt
     9721 taccctgcgt aacggctcag caaaaggaat gccgtatgtg aaatgttacg ccgagaagat
     9781 catgcatgtg cgcgatgcgc aggtcacgcc aatgcatttt cactggcgta agcgtgagga
     9841 catcatcaat cgcggcggcg gcaatttgat tgttgaactg tggaatgccg atagcaacga
     9901 gcaaacagtt gataccgacg ttacggtggt gatcgacggc tgccgccaga ctcatgtcgc
     9961 aggcagtcag ttacgcctgt cccccggcga aagtatctgc ctgccgcccg gcctgtatca
    10021 cagcttttgg gcggaaaccg gttttggcga tgttctggtt ggcgaagtct cttctgtcaa
    10081 tgacgacgac cacgataacc actttttaca accgctggac cgctacaacc tgattgacga
    10141 agacgaaccg gcccagctgg tgttgtgtaa cgaataccgc cagttcctct gaataaggat
    10201 aacgttatgc cgttgatttc tctcgctgaa gggctggcac atgcccgcga acatcactat
    10261 gcgttaggtg cgtttaatgt gctggactca cactttttac gcgccttgtt tgctgctgcc
    10321 agacaagaac gttcgccctt tattatcaac attgccgaag tacattttaa atatatctcg
    10381 ctggattccc tggtcgagac agtgaaattt gaagcggcgc gtcaagcgat tccggtggta
    10441 ttaaatctcg atcacgggct gcattttgaa tcggtggcgc aagcgttgcg gctgggattc
    10501 agctcggtaa tgtttgatgg ctccacgctg gggtatgaag agaatatccg ccagacgcgg
    10561 gaagtggtga aaatgtgcca tgccgttggc gtatcggtag aagctgaact gggcgcggtg
    10621 ggtggtgacg aaggcggtgc gctttacggt catgccgatg aatctctgtt taccgatccg
    10681 cagctggcgc gtgattttgt cgaccgtacc ggcattgacg cgctggccgt ggcgattggc
    10741 aatgcgcacg gcaaatatcg cggcgaaccg aaactcgatt tcccgcgcct cgacgctatc
    10801 cgccagcagg cggctatccc gctggtatta cacggcggtt cagggattag agatgccgat
    10861 ttccgccgcg ccatcgaact gggtatccac aaaatcaatt tctacaccgg catgtcacaa
    10921 gcggcgctgg ctgccgttga ggcccgaatg gcgcaccgtc agccgatgta tgacgagttt
    10981 gccgagttac tactagcagt ggaagccgcc atcagcgata cggttgcaca acagatgcgt
    11041 attttcggca gtgcggggca ggcatgatgg aacgtaaagg gattatcgcc gcaggcaata
    11101 tgctggttga ccacgtccac cagattatcc agtggccaga gcgcggctgg ctggcggaga
    11161 ttacccacag cgaacgctcc accggcggcg cgccgcttaa cgtcctgctg acactggcga
    11221 aaatgcgcac cgggttgctg ttacaggcgg tcgggctaat cggtcaggac agcgacggcg
    11281 attacattat ggcgatgctt gagcaatatc acgtcaatcg tcagcatgtg cagcgcacta
    11341 cctttgcccc gacgtcaatg tcgcaggtaa tgactgatcc aagcgggcag cggacgtttt
    11401 tccattcgcc aggtgcaaac agacttttgg atcttccggc gttcgatcag ctcgataaca
    11461 ccatgaagat tttccatctc ggctatttac tgttgctcga cagcctggat atgccggacg
    11521 atgtttacgg cacccgtagc gcacgcctgc tggcgcaaat gcgtgatact ggctttgaaa
    11581 cctcactcga cctggtctcc cgcaagggcg atccgcgtta tcagccgctg gtgttgcccg
    11641 cgttgcgcca tctggattac ctggtaatta acgaactgga ggccggggag tttagcgggc
    11701 tggaaattcg gttacccaac ggcgaaccgc atatcgcgca tattgctgcc gccgcgcgtg
    11761 aactgttaga tgcgggcgtg cgccagcgtg tggtgatcca ctgcccggaa ggggcgtggg
    11821 gagagacccc ggaacagtgc ggcgtgtgga tcccttcgca aaagttagaa caacgggaaa
    11881 ttatcggcag cgtcggtgcg ggcgatgtat tttgtgccgg atttttatat ggctgccatg
    11941 aacgctggac gcttaccgag agcatcaaac tggcacacgc ctgcgcgcga gcaagcctgt
    12001 tatgcgccaa tgccatcgac ggggcgaaaa cgctggaaga gctgcaaaca gaaatgtcgg
    12061 attaatgcac gtcggcagcg aagacatacc ccagaccgcg cagagtttta atcaacatcg
    12121 gctgatgcgg gttgagttcg attttgcgtc gtaagcgcat gattaatacg tcgatggtgc
    12181 gatcaaacac ctccacgctg tcgctatgag tgaacgccag cagctgttcg cggcttaata
    12241 cccgacgggc attttgagcc agggccagta acaagccata ttcgccctga gtcagctcga
    12301 catgttgttg tagcggacta tgcagttggc agcgaatggt atcgagatac cagccattga
    12361 atgacagccc ggcgtttttc tcctcattaa ccgccagcgc gccaacccgt cgcaacacgc
    12421 tttcagtcgt gcgaccacca cccgtggggt gaacggcttg cagatgtaat catccgcgcc
    12481 catttccagc ccgaccacca catcagactc actccccatt ccggtcaaca tcaccacagg
    12541 caatgccgga cgcagtttct ggatttgctg caataccctc aggccgttgg tatcgggcag
    12601 catcatatcc agcaccacca gcgcaatgtc aggctcgccc tcaatacgcg atatggcctc
    12661 ctgtccggta tggctgactg aaaccgtaaa gacatgttca cttaacacgt cctgcaacag
    12721 tgcgcagata gccgtgtcat catccaccac cagcacgacg ggtttcatgg ttacactccg
    12781 catcattcat ctaattaatc attgtgcgta cttcttgtcg aagtgaaagc ggaaagtaat
    12841 ataaataata aaaatataag tcaggtcaca ttattgatga tagtaataaa tatgccgtgc
    12901 ttttccctct tcagcacaaa tgcctaataa aatggcgcgt tccgctcaca cttccactta
    12961 ttaaagtcgc aaattcccaa ttatgataac cccagcttta aatgacgtat tttgcatgga
    13021 ttacatattt acgacacaaa tgataaatgg agatttatat gaataactct cggttattcc
    13081 gtttgagcag aattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta
    13141 acgcgaaaga ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg
    13201 ccaaatcatt accgtttagc gatcgtcagg attttgacga cgctcagcgt ggatttatcg
    13261 ctccgctgct ggatgaaggt attctgcgcg atgctaatgg caaaccatat tatc
//
LOCUS       DADLTI010000113        13161 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLTI010000113 DADLTI010000000
VERSION     DADLTI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.113"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            34..435
                     /locus_tag="JN755_002935"
     CDS             34..435
                     /locus_tag="JN755_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY7381668.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JN755_002936"
     CDS             447..818
                     /locus_tag="JN755_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381669.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JN755_002937"
     CDS             805..1395
                     /locus_tag="JN755_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381670.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JN755_002938"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JN755_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JN755_002939"
     CDS             1802..2542
                     /locus_tag="JN755_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381671.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JN755_002940"
     CDS             2601..2987
                     /locus_tag="JN755_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY7381672.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JN755_002941"
     CDS             2996..3325
                     /locus_tag="JN755_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY7381673.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..6236
                     /locus_tag="JN755_002942"
                     /pseudo
     CDS             3297..6236
                     /locus_tag="JN755_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6236..6565
                     /locus_tag="JN755_002943"
     CDS             6236..6565
                     /locus_tag="JN755_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381674.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6565..7263
                     /locus_tag="JN755_002944"
     CDS             6565..7263
                     /locus_tag="JN755_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7381675.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7268..8011
                     /locus_tag="JN755_002945"
     CDS             7268..8011
                     /locus_tag="JN755_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381676.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7948..8549
                     /locus_tag="JN755_002946"
                     /pseudo
     CDS             7948..8549
                     /locus_tag="JN755_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8610..12089
                     /locus_tag="JN755_002947"
     CDS             8610..12089
                     /locus_tag="JN755_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY7381677.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            12157..12756
                     /locus_tag="JN755_002948"
     CDS             12157..12756
                     /locus_tag="JN755_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7381678.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12820..>13161
                     /locus_tag="JN755_002949"
     CDS             12820..>13161
                     /locus_tag="JN755_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381679.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQ"
BASE COUNT         3088 a         3101 c         4194 g         2778 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattagtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggtg ttaatcagtc
     3961 agatggcgcg cggcaatttt ggtgcgctga ggggatccgg gataacgctg gctaaccgtg
     4021 ccggatggac aggcgcactg atgtcgccga aaggcatgat gactggcggc gttattggcg
     4081 gacttgtcgc ggcggtcctg ggtctgggta aagcctggca tgacggcgga aggagggcga
     4141 ggaatttaac cgtcagctgg cgctgacggg acattatgcc ggtgtcactg tcgggcagtt
     4201 gtggaaactc agccgggcca tatccgggaa tggtatcacg caacatgcgg cagccggtgc
     4261 gctggcacag gtggtgggca gtggggcatt tcatggaaac gatattggta tggtggcgaa
     4321 agctgccgca cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt
     4381 taagcggctg aaggatgatc ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt
     4441 tttgactgcc actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga
     4501 tgcggcccgg atcgccatgt ctgcgctggc agaggaaacc ggtaaacgca catctgatat
     4561 tgataataat ctcaatgcgc tgggtagtac gctgcaaacc ttgtctgact ggtggaagca
     4621 gttctgggat gcggccatga atattggtcg tgaagactcg ctggatgcgc agattgatgc
     4681 gttacaggaa aaaattcagc gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt
     4741 ggagtacgat cagcagcgtc ttaacgatct tcaggagaaa aaacgccgga aggatttgca
     4801 ggatgcaaaa gcgcaggcag aacggaatta ccaggagcaa cagaaacgcc ggaatgctga
     4861 aaatgccgcg ctgaaccgga tgaatgaaac ggaagctgca cgacatcagc gggaaattgc
     4921 gcgtattaat gccatgcagt acgccgacca ggctgtcagg gatgcggcga tacagcgtga
     4981 aaatgaacgt tacgaaaaag ccattaagaa aaatacacgg gcaacccgta atgatgaggc
     5041 cacccggtta ttgctgcagt acagtcagca gcaggcacag gtggaagggc agattgctgc
     5101 tgccagacag tcagcaggca ttgccactga aaggatgact gaagcgcata aacagcttct
     5161 ggccctgcag cagcgcatca gcgacctgga cgggaaaaag ctgacggcag atgaaaagag
     5221 tgtgctggcc cgtaagaatg agctgattca ggcgctgacg ctgctggatg tgaaacagca
     5281 ggagctgcag aaacagacag cgcttaacga cctgagaaaa aaaacggttc agctgaccag
     5341 ccagctggca gacaaagaac gtgcactgcg tgagcagcac aatctggata ttgccactgc
     5401 aggtatgggg gataagcagc ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata
     5461 ccggcaacag ttgcaacagc ttgagaatga cagtcgccag aaaggcactt acgggacgga
     5521 ggactaccgg agggctgagg aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa
     5581 caaacgctac tggcaggaac tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg
     5641 ggcgtttcag aattttacgg cggatgcgga taatgcggcg ggaacggcag aacagatgtt
     5701 tacagtggca ttcagcagtg ccggtaatgc actggcgaca ttctgtacca ccggtaagct
     5761 gaattttaaa tccttcacct cttccctgtt gtcagatatg gccagaatta tggcacagat
     5821 ggccatgatg caggcggtaa agggcgtcgg ttctttattc ggcttcacga ctaatgctga
     5881 tggcggtgtt taccagtctg ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc
     5941 gacgtttttt gcttttgcaa aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat
     6001 tctgccactg cgtcgcggtg ctgatggtaa gctggggggt tgtggcggat actggtggtt
     6061 caggcatggt gatgtttgcc ccgcagtaca acattgagat caataacgac ggcacgaacg
     6121 ggcagatagg tccggctgcc ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg
     6181 actttatgca acagcaggcc cgtgatggtg gccggttaag tggagcatac cggtaatgga
     6241 gacgtttcac tggaaagtgc gcccggatat gaatgtggta tcagagccga aagtggtgac
     6301 agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg ggactgaata accagttgtc
     6361 gacttacagc gtgacgatac gtgttcgtaa aggtgaacac ccatctttaa aagcctttct
     6421 ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca ccttatgact ggaaactgat
     6481 cagggaggtt tgtcgtaaat ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc
     6541 cgattttgaa caggtggtga actgatgcag gatatccggc aggaaacact gaatgaatgc
     6601 acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa tcgatctgac agaggtcggt
     6661 ggtgagcgtt attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag
     6721 gggcggcagt atcaggcata ccccattcag ggggcggggt ttgaactgaa cggcaggggc
     6781 tgtgctgccc gtccgacact gacggtctct aacctgcacg gcatggtcac cgggatggcg
     6841 gaagatctgc agagtctggt cggcggaacg gtggtccggc gtaaggttta cgaccgtttt
     6901 ctggatgcgg tgaacttcgt caacggaaac agcgacgccg atccggaaca ggaggtgatc
     6961 agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg tcagtgcctc ttttgtgctg
     7021 gccacaccaa cggagacgga tggcgcggtt ttcccggggc gtatcatgct ggcgaatacc
     7081 tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg gcagggcagt ggctgacgag
     7141 ttcgacaaac caacgacgga tatccggaag gacaaatgca gcaagtgtat gcgcgggtgt
     7201 gagttgcgca acaataccgg taatttcggc ggtttccttt ccatcaataa actttctcag
     7261 taaatccatg acacagacag aatcagcgat tctggcgcac gcccggcgat gtgcgccagc
     7321 ggagtcgtgt ggattcgtgg tgagaacgcc ggagggagac aggtatcttc ccagcgagaa
     7381 tatctccggt gagccggagg aacggttccg gatggctccg gaggactggc tgcgggcaca
     7441 aatgcagggt gagattgtgg cactggtcca cagtcatccc ggtggtctgc cctggctgag
     7501 tgaggctgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg tctgccgggg
     7561 ggcgattcac aagttccgct gtgtgccgca tctcaccggg cggcgctttg agcacggggt
     7621 gacggactgt tacacgctgt tccgggatgc ttatcatctg gcggggattg agatgccgga
     7681 ttttcaccgc ggggatgact ggtggcgtca cggtcagaat ctctatctgg ataatctgga
     7741 ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa ccgggcgatg tgctgctgtg
     7801 ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgcggcgatg gtgagctgct
     7861 gcaccatatt cctgaacaac tgagtaaacg agagaggtat accgacaaat ggcagcgacg
     7921 cacacactcc ctctggcgtc accgggaatg gcacgcatct gcctttacgg ggatttgcaa
     7981 cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg ctgaagccat ccgcgcactg
     8041 gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg gctggtatca ggtgcgcatt
     8101 gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc ttaatgagcc gctggcaaat
     8161 ggtgcagtga tccacatagt accgcgtctg gtgggagcta aaagtggcgg tgtgtttcag
     8221 gcggtgctgg gggcagctgt tatggcggtt gctatatgga tgccggggta ggaattatgg
     8281 cgagtaatct gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc
     8341 tggcaccgaa agccagaact ccccgtacac agacaacgga taacggcaaa cagaacacct
     8401 gtttctcctc actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg
     8461 aaatgcgcgt ggggtcgcgg gtggtatctc aggagatcag cacggcagac gaaggggatg
     8521 gtggtcaggt tgtggtgatt ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg
     8581 gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc
     8641 cgcgcgaagc gaaggacaac ctgaaatcca cgcagttgct gagtgtgatt gatgccatca
     8701 gcgaagggcc ggttgacggt ccggtggatg gattaaaaag cgtgctgctg aacggtacgc
     8761 cggtcctgga cagcgagggg aagacaaact tttccggtgt tacggtggtg ttccgcgccg
     8821 gcgagcagga gcagacaccg ccggaggggt ttgaatcttc cggctcagag actgtgctgg
     8881 gtacggaagt gaaatacgac acgccgatca cccggaccat cacgtcggca aacattgatc
     8941 gtctgcgcct gaccttcggt gtgcaggcac tggtggaaac cacctcaaag ggggaccgga
     9001 atccgtcgga agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa
     9061 aagacatcac cattaagggc aaaaccactt cgcagtatct ggcctcggtg gtggtgggta
     9121 acctgccgcc gcgcccgttt aatatccgga tgcgcaggat gacaccggac agcaccacag
     9181 accagctgca gaacaaaacg ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt
     9241 gctacccgaa cacggcgctg gtcggcgtgc aggtggattc agagcagttc ggtaaccagc
     9301 aggtgagtcg caattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc
     9361 cgcagacgcg gcaatacagc ggtatctggg acggaacgct taagccagca tacagcaaca
     9421 acatggcctg gtgtctgtgg gacatgctca ctcatccgcg ctacggcatg gggaaacgtc
     9481 ttggtgcggc agatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt
     9541 cagtgccgga cggatttggc ggcacggagc cgcgcatcac ctgtaacgct tacctgacca
     9601 cacagcgtaa ggcgtgggat gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg
     9661 tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataaa gtgtggacct
     9721 ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg
     9781 ccctgaagga ccgccataat gtcgttgagg tgaactggat tgatccggac aacggtcatg
     9841 agacggcgac agagcttgtg gaggacacgc aggccattgt ccgttacggt cgtaacgtca
     9901 cgaagatgga tgcctttggc tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc
     9961 tgattaaaac ggaactgctg gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc
    10021 ttcgccatgt accgggtgat gtcattgaaa tctgcgatga tgattatgcc ggtatcagca
    10081 ccggtggtcg cgtactggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa
    10141 tcacgctgcc gtcctccggt accacgctga taagcctggt tgacggaaat ggcaatccgg
    10201 tcagcgtgga ggttcagtcc gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg
    10261 acggcgttgc agaatacagc gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt
    10321 tccgctgcgt gagtatccgt gagaacgacg acggcacgta tgccatcacc gccgtgcagc
    10381 atgtgccgga aaaagaggcc atcgtggata acggggcaca ctttgacggt gaccggcgcg
    10441 gcacggtgaa tggtgtcacg ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag
    10501 acagcgggga atatcaggtg ctggagcgat gggacacgcc gaaggtggtg aagggtgtga
    10561 gcttcctgct tcgcctgacc gtggcagcgg acgacggcag tgagcggctg gtcagcacag
    10621 gcaggacgac ggaaaccaca taccgcttca ggcagctggc gctggggaat tacagtctga
    10681 cagtccgggc ggtaaatgcc cgggggcagc agggcgatcc ggcgtcggta tcgttccgga
    10741 ttgcggcacc ggcagcgcct gtcactattg aactgatacc ggggtatttt cagataacgg
    10801 tggtcccgaa actggctgta tatgacccga cggtgcagtt tgagttctgg ttttcggaaa
    10861 agcggattgc ggatatcagg caggttgaaa ccagcgcccg ctatcttggc acggcgctgt
    10921 actggatagc cgccagtatc aatatcaggc cgggccatga ttattatttt tacgttcgca
    10981 gtgtgaacac cgttggcaaa tcggcatttg tggaggccgt cggtcgggcg agcgattatg
    11041 cggaaggtta tctgagtttt tataaagggt tgatcaataa aacgcatctc ggcaaggagt
    11101 tgtggacgca gattgataac ggtcagcttg cgccggacct gactgaaatc aggacgtcca
    11161 ttacgaatgt cagcaatgaa atcacgcaaa ccgtcaataa aacactggaa aatcagagtg
    11221 ctgcaatcca gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt
    11281 gggctgtgaa gctgcaacag atgaaggacg gacgccttta tattgcgggt atcggtgccg
    11341 gtattgagaa tacgccagca ggaatgcaga gtcaggtgct gctggcggca gacaggattg
    11401 cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga
    11461 tattcatgaa cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc agcggcggta
    11521 atcctccggc attttccctg acacctggcg gacggctgac ggcgaaaaat gccgatatca
    11581 gcggtaacgt gaacgcgaac tccgggacgc tcaacaacgt cacgactaat aagaactgtc
    11641 gggttctggg aaaattgtcc gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca
    11701 aacctttccc acgggactcc cgggcaccgg agaggtggcc atcagggacc attaccgtca
    11761 gggtttatga cgatcagccg tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg
    11821 gtgctaaaca tgagcggaag aataacaata tttattcgtc atgccgcctg atagtgaaga
    11881 aaaacggtgc tgaaatttat aaccgaacga ccctggataa tacgctgata tatacgggtg
    11941 ttattgatat gcctgccggt cacggtcaca tgacgctgga gttttctgta tcggcatggc
    12001 tggtaaatgg ctggtatccc acagcaagta tcagcgattt gctggttgtt gtgatgaaga
    12061 aagccactgc aggcatcacg attagctgaa ttttataacc cagatacggg caccagaaat
    12121 ggtgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg tgcagccatt
    12181 ttgtccgcag ccatctgtct ggctgtatcc ggtgcgcctg catgggcgtc tgaacatcag
    12241 tccacgctga gcgccgggta tcttcagccc catactgata tgccaggcag cgatgacctg
    12301 aagggcatta acgtgaaata ccgttatgag tttacggaca cgctggggct ggtgacgtca
    12361 ttcagctatg ccaacgctga agatgagcaa aaaacgcatt acagcgatac ccgctggcat
    12421 gaggattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat
    12481 gaatggttca gcgcgtatgc gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc
    12541 ggggattatc tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccgggagt
    12601 gatgacaacc gccacagcaa cacgtctctg gcgtgggggg ctggtgtgca gtttaacccg
    12661 accgaatccg tggccattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
    12721 gacggtttca ttgtgggtgt cggttataag ttctgattag ccaggtaaca cagtgttatg
    12781 acagcccgct ggttcaggcg ggctttttgt ggggtgaata tggcagtaaa gatttcaggt
    12841 gtactgaaag atggggcggg taaacctgtc gtaaattgtg cgattgaact gcgggccaga
    12901 agaaccagtc cgaccgttgt ggcacacgtt gttgccactt gcgtgacgga caataacggt
    12961 gcttatgtga ttgaggctga gccgtggtat tacgaggttg cgcttcactg taacggctgg
    13021 cagccaaccc gtgtcgggga tattgatgtg gcaccgactg atgcaccggg gacactgaac
    13081 gcgtttctga atgcaccaaa ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag
    13141 gaaatggtgg cgcaggcgca g
//
LOCUS       DADLTI010000114        12958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLTI010000114 DADLTI010000000
VERSION     DADLTI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.114"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(8..355)
                     /locus_tag="JN755_002950"
     CDS             complement(8..355)
                     /locus_tag="JN755_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7381680.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(446..790)
                     /locus_tag="JN755_002951"
     CDS             complement(446..790)
                     /locus_tag="JN755_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381681.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVDLLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(817..984)
                     /locus_tag="JN755_002952"
     CDS             complement(817..984)
                     /locus_tag="JN755_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381682.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(1172..2011)
                     /locus_tag="JN755_002953"
     CDS             complement(1172..2011)
                     /locus_tag="JN755_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7381683.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(2013..2699)
                     /locus_tag="JN755_002954"
     CDS             complement(2013..2699)
                     /locus_tag="JN755_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7381684.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(2686..3000)
                     /locus_tag="JN755_002955"
     CDS             complement(2686..3000)
                     /locus_tag="JN755_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381685.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2997..3482)
                     /locus_tag="JN755_002956"
     CDS             complement(2997..3482)
                     /locus_tag="JN755_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381686.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(3479..3643)
                     /locus_tag="JN755_002957"
     CDS             complement(3479..3643)
                     /locus_tag="JN755_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7381687.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(3654..3947)
                     /locus_tag="JN755_002958"
     CDS             complement(3654..3947)
                     /locus_tag="JN755_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7381688.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3961..4467)
                     /gene="ssb"
                     /locus_tag="JN755_002959"
     CDS             complement(3961..4467)
                     /gene="ssb"
                     /locus_tag="JN755_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY7381689.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(4468..5175)
                     /locus_tag="JN755_002960"
     CDS             complement(4468..5175)
                     /locus_tag="JN755_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY7381690.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(5430..5582)
                     /locus_tag="JN755_002961"
     CDS             complement(5430..5582)
                     /locus_tag="JN755_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY7381691.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(5567..5698)
                     /locus_tag="JN755_002962"
     CDS             complement(5567..5698)
                     /locus_tag="JN755_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY7381692.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5723..6691)
                     /locus_tag="JN755_002963"
     CDS             complement(5723..6691)
                     /locus_tag="JN755_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HAY7381693.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6859..7101)
                     /locus_tag="JN755_002964"
     CDS             complement(6859..7101)
                     /locus_tag="JN755_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381694.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(7304..7636)
                     /locus_tag="JN755_002965"
     CDS             complement(7304..7636)
                     /locus_tag="JN755_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7381695.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(8097..8501)
                     /locus_tag="JN755_002966"
     CDS             complement(8097..8501)
                     /locus_tag="JN755_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381696.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(8498..9130)
                     /locus_tag="JN755_002967"
     CDS             complement(8498..9130)
                     /locus_tag="JN755_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7381697.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            9234..9449
                     /locus_tag="JN755_002968"
     CDS             9234..9449
                     /locus_tag="JN755_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7381698.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            9569..9862
                     /locus_tag="JN755_002969"
     CDS             9569..9862
                     /locus_tag="JN755_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381699.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9895..10041
                     /locus_tag="JN755_002970"
     CDS             9895..10041
                     /locus_tag="JN755_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 domain-containing protein"
                     /protein_id="HAY7381700.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            10034..10867
                     /locus_tag="JN755_002971"
     CDS             10034..10867
                     /locus_tag="JN755_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAY7381701.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10857..12293
                     /locus_tag="JN755_002972"
     CDS             10857..12293
                     /locus_tag="JN755_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7381702.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            12370..12810
                     /locus_tag="JN755_002973"
     CDS             12370..12810
                     /locus_tag="JN755_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7381703.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12807..>12958
                     /locus_tag="JN755_002974"
     CDS             12807..>12958
                     /locus_tag="JN755_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7381704.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIR"
BASE COUNT         3126 a         3185 c         2890 g         3757 t
ORIGIN      
        1 tctgctttca gacgatcgtg aagttcttgc gcttgccttt tgtccgatgt ttcaagagag
       61 cgtctaactc ttgatccatc tggcgcgacg aaatcgcagt gccacgtgcc accgcgtagt
      121 ttgattgaca tgctttaacc tcctgcacat caaccgcatt caccgcgcta ttgtgtctca
      181 cagacttaag cgccgcaatg cagtctgact tgcaaatgcg atatgggctt ttaggtttat
      241 ctggatttat ctttacggcc tgaagtcgtc cacttcgtat ccactgcgtg atagtgcctt
      301 tgtctacctt cagatacgac gctgcctctt cacgagtgaa gatttcttct tccacctgga
      361 atctccattt attggattgg tattattgcg gtaggtctgg atatcattga gcaatgaaca
      421 ggcctcatcg agtatgaggc tgtggttagt ccttgcgtaa ctcgctaatt cttctgtaag
      481 tctctggtgc tttgtttccg tgtatcttca tttcagactt caacagatca acgagggaat
      541 cccattcgtt gaggatgcct ttgaatgccg gaacgcgctt tgcaaccttg tcgaatgaat
      601 ctctgatttc tgggatctgc tcaacaagtg caacgcatcg tctgaaatcg gctgcgtcat
      661 ggggagcgcc gaagtgatga ccatagatat tctttttcag tccacatgcg attgaggcaa
      721 gagttgcgct actgatgcca acatcgccag tcgattgcca tttcaaaacc ttcatagcca
      781 aatctgacat ttcttgtctc caataaaaaa ccgccatcag gcggcatggt gttctttcag
      841 ttcctcaatt cgaatattgg ttacgtctgc atgtgctatc tgcgcccata tcatccagtg
      901 gttatagcag tcgttgatgt cctctgcttc gataaccctg tcgaatggct ctccattcca
      961 ttcacctgtg actcggaagt gcatttatca tctccataaa acaaaacccg ccgtagcgag
     1021 ttcagataaa agaaatcccc gcgagtgcga ggattgttag ttgctatctg ctgcttcctt
     1081 ggtcattacc atatccaccc aatttcacca gacatgattc tcgcaatcac tattatcacc
     1141 aaagtgataa tcacaacttt aactggcggc atcattcacc atcctgctcc ggcggttcta
     1201 gcagcggcat ccagtgggtt acgtcatcca agatatttcc tgataaatat gtgaaagctc
     1261 tatatttttt gtaatcaatt ggactcatta cccagttcca atatgctgcc actatttgac
     1321 cttgactaaa tgccagtaac attttggtgt cttccggcat tcgctcacta cagcttatcc
     1381 aaccatccgg agttaccgga tagttgcgca ttgcgacctt taatgcctca tagaagcaac
     1441 ctttcagatt gttgaactga cgcccattta gcgggccatg ttcagtcagc atgttgtgta
     1501 atttccatgc cgcgtcgttt acttcgttgg atgacagggg aggcaacttg taagtttggc
     1561 ttacaggttc ggcaccatga agcatggcgt cgctccgctc tatgccatcc agcgcgattc
     1621 gtagtgcctg aattgtggta gagctatcgt ttggggctat tccatatcgc tcgaatacag
     1681 ctaaatggtt gcgcataatc tcaggcgtaa gctctttgta agcataagcg agaggctctg
     1741 atgcattatc cggcacaacc gacgcaggcg cggcagcata aacaggaata acgtccgctt
     1801 gctctttatt gctttcatcc gttaaagccc agaataattt cccggccgga tgtttgaaaa
     1861 tataagcaac tggctctgct tccagcgatg ccagtgcaat tcgtgccagt tcttccgctt
     1921 cttctgctgg cagtacaacg ttgctaccag gtccgtatgt ttcgcgccac tgcttgattg
     1981 tcagtagtcg ctctttggta atagtggtca tctcactctc ctttgatgcg aataccagta
     2041 gcgcggattg catcgatgac ttcagaaact ttgtatgcca ttaccgtttg gtaatcctcg
     2101 tgaaaatctg ttcgatgaag catgctgcta cgttccggga gcagtatttc ccgctcctcc
     2161 agttctgcta tgcgcttctc tgcggattcc agcttctcgc gcatatcgtc aacgtactcg
     2221 accagagaac cgccagcagg aatttcgcac tcctcgacca gttggaagta gatatcagct
     2281 gcggcccgtg tgttgctata cctagcgtcg cccatctcac cttcacgaag agcatcgcgt
     2341 tcggcggtaa gattggctat tttgctgtct ttgccttcca gctcaacgcg cagcttccct
     2401 accgtaagcg caatatcctc gttctcctgg tcgcggcgtt tgatgtattg ctggttcctt
     2461 tcccgttcat ccagtagtgc ctgcgctact tcagggttga aagctgcgat ataacgagcg
     2521 ttgttctctg cgttgttctg tccatcaaag ccggtccatt tgataacgtc ttcacatcgt
     2581 ttatcaccag gtgtatgcac cgcatatgta ccagtaccag acgaaataaa tgcgacccat
     2641 tcaccctgtg ttgccagttg cgccgcttta cgcagtgcct ggtaattaat ttcgctcact
     2701 ggttgcctcc tttgctgggc tttctaactt ttgagtggtt gtatcaaatt caaacaactt
     2761 aaccacgtca tcaaacagga cataatcacc atcagaatct tcagtcatgt cagcgccaca
     2821 atcctgaccg catgagtcac aaccacccat atcaagctcg tatcgcttca ggtttgcgat
     2881 atttgataaa ttcagcgcca gtacagccag gtcataaacc tcttcggcag tgacatcgct
     2941 gctcagtccc atttcatggc gatatatgat tttttctact cgttgttttg tgattgtcat
     3001 ttttgctcgc ctccctgttc ttccagaaaa atacgcatag tctcaagcat ctcttcggtg
     3061 tcatacggtg acaacttgtc acgcaggatg tgttcaatgc tgttaatgaa cttgcggatt
     3121 gctttgcgtt caatttcagc cagaaaagca tcggttgccg gggtttcagg tttaatggct
     3181 tcaagcacgg cgcgaatgac atcggtatcg ttctccaccc atgaccattc gcttgtatca
     3241 ttccagtcgt ggtctatgat agctgtttca ttgaatgcct cgactgccgt atccgggatt
     3301 acttcatgat ttaaagctgc cttcagcccc gcattctccg ccgccagcgc cgcgcacttg
     3361 gcctccgctt cagcaaattt acgcaccaga tattcagcgt ttgtttcgtt aacctttaaa
     3421 tcacttggga tgcatttacc tttcagaaat ccatccatct caattagtgt catttgtttc
     3481 atttcttccc attccgccac attgcattca gatatttgtt ttgatttact gacggaaaag
     3541 aatttctttt aagcaattcc tctctcgatg gcattggctt tacgcgttgg cgaataatca
     3601 tttctgccgg aagaatgccg agattgtatg caagtcctct catggtaaat tcctcagtca
     3661 ttactgatag cgccatagcg tgagcggtaa ttacgcaggc gcgggtcaat ttcagggaag
     3721 tgggtatatg tggctttgcg gaatggtcgg attgatgtct ggtaaattcg ctcgcgctct
     3781 tctttctctg caagccatat gcagtggcga aattcctttt cctctttcgt ttcctgcggt
     3841 agcgacatta tccggtcgta gtttttcctg aatttatcca gcacctccga tacggaattg
     3901 ccggaacagc ggcgcgggtc atccgcacca tactgaggcg ctggcatgat tttctcctga
     3961 ttaaattgcg tgaatagcgt gacgagggaa ggggagagtt actggtgcaa agggtatatc
     4021 gtcgtcaaaa tccatcggag gttcgttgtg ttgtgctggt gatgattgct gctgtggctt
     4081 ctgtgattgc ctgctggctg cttgttgttt gctgtcgcca atgccgccaa gcatttgcat
     4141 cacgccatta attccgacat gaacctcggt tgtgtaacgg tcttgccctg actggtcttt
     4201 ccactttctg gttctcagca ttccctcgaa ataaatctga tcaccttttt tcacatactg
     4261 ccccacgacc tcagccagtt tcccggatac agcaacacga tgccattcag tcaattcctt
     4321 ttgctcgcca gtatttttat ctcgccattg ttctgacgtg gctattgtca ggttagcgaa
     4381 cgctgttcct gatggtgagt atcgaacttc cgggtcttgt cctacccgac caaggataat
     4441 caccttattt acgcctctgc ttgccattta tgccgcctgt tttagttcgt taactctgat
     4501 gttcattacc tgaacgcatt tagcctgcgc ttcctcgttg ccagccatta attgccagtc
     4561 acgctgataa cgctcgatga gttttttctt gtcagtttct gttgacgcat aatcgctgaa
     4621 gtctttcagg atttgttcgc agtcaaccga tggagatttc tggttggtat tttctggtga
     4681 tggtttgtta tctgatgctg ggattgccca tcccggcagc gatggaggga gccagtaaaa
     4741 tcctgttcca tccttcagtt ttgccctgtg ccacccatgc tttttatcga gagatgtttg
     4801 tgcgaaacct tcctcaaggt tatacagata ccgaccgatt ccccactgaa cggcagcacg
     4861 cttcattgca cctgaacgac cacctttgac ggcttctacc tgcgtgtttt cagcagcatc
     4921 ccatttggtt acccattcgg aatcaatcct gattgatatg ccgcattcaa cgcctccgtt
     4981 gttgggaata tcgcggtatt cattgcgcca tcctgctttg ccgcaaacat cgtccaggcg
     5041 tttcatgatt gcccggttcg tgacataagc cagcaccata gcccacactt tgccatcgcg
     5101 tgttttaccg ctttgctgta ttcgccattc gatatcttca gggctgaatg gctcatcgaa
     5161 tttattcaaa tccataaatc acctcagaat ggacatggcc caagaaaata acgctgattt
     5221 aatacttcga ctcgggacaa attaaggcat acccgcattc cttcgcggtc gccattatgg
     5281 cgataccaga gagctttctg ggtgtacatg cgtctctgta acttgctctc cttcactgtg
     5341 gttgcaagtg acatgaatat ctccttcgtt tccgattaat tctttcatct gacgaatgaa
     5401 ttcttcgtct gaccagttat ctgtaaaact catttcctgc gataccacgg aaggttgata
     5461 gctgacttca tcgctttatt tgcttcaagc cacattttgg aatcaccaat aaatctggct
     5521 attactgctt tgttctgtgc agcacgaagc atctggtgat tgatggctat ttcattgcgc
     5581 ataataagac atcaactctt ttccatccgt cacgtaattt acgggtgatt cgttcaagta
     5641 aagattcatt taattggaag gcacccatgc gagcgcctcc cgcgattgcg taaatcatgg
     5701 gtggttcctt atgttggttt tattagtagg ttatttttgt tgcgaatact tcgcctttta
     5761 cgatggctgt tatgatattt ttagcaacat cttctgatgc gccaaccttg ataaggtcag
     5821 caagtatttt gttatttact tcttttcggt gagctttatc ctttgctcta cgctcttctt
     5881 cgtccttgat tcttttttct tctgctattc tggcttgctc ttttgcttca gcttcgcgcc
     5941 ggattcgttc agcctcctcc tgtgcttttc ggcgttctgc ttcaattgcc gcctgctttt
     6001 ctctttcagc tcgttctgct gcctcttttg cttcgcgctg tgctcgttgc tcggcttcaa
     6061 tgcgttcacg ctttgcacgt tccgctgcgg ccttagcttc tgcttctcgc cttgctgctg
     6121 cttcaatttc ggcttttgcc tttgcttcgg cttctgctct ggctttctct tcagcttctc
     6181 tttttaagcg ttcttcatgc tctcgctttt cctgctccgc tttgagtctt gcctcttctc
     6241 tttggcggtc aaattcgcga tccatcaaaa tcgctatttc atggtcagac tcaatttgct
     6301 ttgcgagagc ttcagctgct gccttagctt cttcttcggc tttaatccgc gcctgttcct
     6361 cctcataatc agtaagaggc tggcgcgcct tggctttcag ctcatcaagt cgatcatgca
     6421 ctgttttgcg gttggcatca attagctttg gaatttcctt cagttcagca acaaggtctt
     6481 tgccaagacc atcgagatat gttttcgtct gcgcaacttt atacgccaga gaagcgatct
     6541 cctttctgcc ctttgccgtt gtgatatcag gcacaaagga cataacttca cgttcaacct
     6601 tttgaaggat ttcttcaatc tggtcggcag actgaaatac agtcattgca tttgcttttt
     6661 caataacaac taaatctgtt acttcactca tatttccccc gtcaaaaaaa ttgccctcac
     6721 actggagggc aaagaagatt tccaataatc agaacaagtc ggctcctgtt tagttacgag
     6781 cgacattgct ccgtgtattc actcgttgga atgaatacac agtgcagtgt ttattctgtt
     6841 gcttattcca aagcaaattt aaccatcaac aaactcttct ggtaatttat caaccagttg
     6901 atgacttatt atcagccatt tgccatcctt cgtttcgtat gcatatttct ggtcttttat
     6961 catcatgtgt tcggctactg ccctcactgc ctgatcggtg acatcttctc tctttcctac
     7021 ccacattcct ttttcagtgt ttaatgttcc ttgaaaaata cgaccactta ataggcttgc
     7081 gcccatagtt tttactctca tgtatcagtc ctcaaataag tggtttgctg cgaaagtaaa
     7141 tacgcttaag ttacctgtta tttatcccac caagttccgt atctatctat ccagttacac
     7201 caatcatcga cactctattt tgttgtgtcg cattttggca actggcaaga atatctacct
     7261 tctttgtaaa gtcggcgttt gactttcttg agcatggctc acctcaatcg taataagctg
     7321 gaattgattt tccgcgttgc ttctggcggc ctgagcaagt cacacctatt tcactgcgtg
     7381 gcttgctgta ccatgtgcgc ttattcttgc gctcagtacg ctgcaggttg ctttcaatct
     7441 gttcgtggta ttcagccagc accgtaaggt ctatcggatt cagtgcgctt tctactcgtg
     7501 atttcggttt gcgattcagc gagagaatag ggcggttaac tggttttgcg cttaccccaa
     7561 ctaacagggg atttgctgct ttccattgag cctgtttctc tgcgcgacgt tcgcggcggc
     7621 gtgtttgtgc atccatctgg attctcctgt cagttagctt tggtggtgtg gtggctggta
     7681 gtctagctcc agcttgttga gtctcattcg gaggggtata accggcaccc cagcgatttt
     7741 tccatgcgac aacgtgcgcg ttatggcggc cttatcgccc gcggctcccc atctcgtcca
     7801 cgctattgct agcgttggga gcgcttcacc gctcaacagt aggtaagcac ttgccagtga
     7861 ctagctggct tcaccacacc ccaaagcctt ctgctttgaa tgctgccctt cttcagggct
     7921 tattttttaa gagcctcacc ttcatggtgg tcagtgcgtc ctgctgatgg cttaaaatta
     7981 caagaaagat tgtatgttgt aaacaagaaa tattgtgaaa aggggcgtga aaaacaaact
     8041 ccattgtttt taaatgaaaa atagtttgtt tttggttatc gagattgagg tggggattac
     8101 tggttgcagg ttccgactac atcaccaaca aatgatttgg ttgatgtaag ttgttgcata
     8161 cctgggatat tcattacttt ggagtaaaga gcttttttgt ctgtagtgat tgaccaagtt
     8221 tcaacggtta ttcctcctcc agactggtat tctcctacca tagtgttcga tgacaaagca
     8281 gtgtatttca tctctggata gacgccagaa actgattcat aaactgatga tttatcgcca
     8341 tttattgtta cgtggaaaac ggaatcttcc gtgctgtctt ttgtaaactc gtaacgatcg
     8401 ccattcattg acccgtaccc gtgcaggttt gtgacaatcc agcattcaga attggcgctg
     8461 gtagttaaga gtattgagag tagcgccgca atcctgatca tacgaatttt accctcgctt
     8521 ccacgacaac accgataatc ttgcagttcc cgttgatagg agtcataggc catgaagggt
     8581 tcaggccttt caggtacttc tgcccgccat ctataaccag tttcttgaat gttgcttcgt
     8641 tcgcgtcagt cagtttggct acaacaaggc ttccattcac tggctcgcgt ccagtatcta
     8701 ctaacaccat atgaccttca gggatgcttt gacctacagg tgaggtcatg gaatcacctt
     8761 caaccttcag ccagaatcca tcgcctaata agttaacgtc actgtcatac cattcatcaa
     8821 tgtccttgat atcgtagggt tcacaagctt cacaccacga accagctcta accatgctaa
     8881 tcaatggata tttccctttg ggttcaacat gcccaacaaa tctaacattc gaatcagagg
     8941 tgccattgag cagccagtca acacttacgc caagagctga cgcaagttct ggtaaaaagc
     9001 gtggtcgctt agttttaccg ttttcgagct gctctataga ctgctgggta gtccccacct
     9061 tttgagcaag ttcagcctgg ttaagtccaa gctgaattct tttgcttttt accctggaag
     9121 aaatactcat aagccacctc tgttatttac ctccaatctt cacaagaaaa actgtatttt
     9181 acaaacaaga tacattgtat gaaaatacaa gaaagtttgt tgatggaggc gatatgcaaa
     9241 ctctttctga acgcctcaag aagaggcgaa ttgcgttaaa aatgacgcaa accgaactgg
     9301 caaccaaagc cggtgttaaa cagcaatcaa ttcaactgat tgaagctgga gtaaccaagc
     9361 gaccgcgctt cttgtttgag attgctatgg cgcttaactg tgatccggtt tggttacagt
     9421 acggaactaa acgcggaaaa gccgcttaag acattcccgc tcttacacat cccagtcctg
     9481 aaaaagggca tcaaattaaa ccacacctat ggtgtatgca tttatttgca taccttcaat
     9541 caattgttat ctaaggaaat acttacatat ggttcgtgca aacaaacgca acgaggctct
     9601 acgaatcgag agtgcgttgc ttaacaaaat cgcaatgctt ggaactgaga agacagcgga
     9661 agctgtggga gttgataagt cgcagatcag caggtggaag agggactgga ttccaaagtt
     9721 ctcaatgctg cttgctgttc ttgaatgggg tgtcgttgac gacgacatgg ctcgattggc
     9781 acgacaagtt gcttcgattc tcaccaataa aaaacgcccg gctgcaaccg agcgttctga
     9841 acaaatccag atggaattct gaggtcatta ctggatcaat ccacaggagt aattatgaca
     9901 aaacaactca gtccttacca ggacaaaatt cacaaacaca tactacgtga tcgcttcctg
     9961 tccagcttca agcagcctgg tcgattccgg gctgagttgg aaaaagtgaa gctgatgcag
    10021 aaggagaaag gtcatgagta acatatctaa tctagccgaa gccagagagg ccagaaggct
    10081 acaacaaccg catcaaagca gcggtaaggg gtatgccttg ctgcaccgta aaattatgga
    10141 tgtgccgttt tacaaggacg cagaagcagc gcatctgtgg gttcacttaa tcctcaaagc
    10201 aaagcatacg cctgagtatg taatgactga cgcaggagaa attctggtag gcagagggaa
    10261 gctacttggc ggtagaaact ctctggcgtt tgaaacagga ctcaaaccag atcgcgttca
    10321 gtacctgctt agaaagttca aaaaactcgg catgattgac tgggtttcac acggtaaatt
    10381 ctcagttttc tcggtagaga aatatgacga ttatcagtca aattttgtac cagcagatta
    10441 ccagcaaatt accacagata aagaatataa taatattatc tctaatactg acgtattaga
    10501 gagtaccgca gcagacaaaa agtctgacaa gaaaaaacct tccgttagct gtcaggatgt
    10561 tgtcgatgct taccacgaaa tccttcctga agcgccaaaa atccgcgcac tgaatgacaa
    10621 gcgtaaaaac cagatccgaa cgttctggcg caaagccgga gtgataaccc gccagcttga
    10681 cgggcatggg ttcacgatgc aggactggag aaattatttg agctacgtag gcgaaaattg
    10741 ccgatggatg ttcgaagagc gcccaaacca tcagcgcgga accgtctggc acaaaaaggg
    10801 atttgatttc ctgcttaacg ataataccta cctgaaagtt cgtgagggtg aacacgatga
    10861 ccgataattt ttatgcgccg ccccatagca tcgaggcaga gcaggcggtg attggtggat
    10921 tgcttctgga tgatgacagc agtgagcgcg tccagaaggt tctggcgatg ctgaagcctg
    10981 attcatttta cagccgacca cacaaaatca ttttcgaaga aataaccaga atgcaccggg
    11041 agcaaaagcc agtagatggc ctgacgcttt tcgatgaact ggagcgtaaa tcgttaacgg
    11101 cgtctgttgg cggttttgct tatatcgctg agatcgcaaa gaacacgcca agcgccgcaa
    11161 acatcgttgc ctatgcaatg caggttcgcg aaaccgcaat ggaacgctac gccatcaacc
    11221 gcatgactga agcgacggaa ttgctctatt cccgcaacgg aatgactgcg acgcagaagt
    11281 acgaagctat tcaggcgatt ttcacgcaac tgacagacca tgcaaaaaca ggatcgcgtc
    11341 gcggccttcg ctcatttggt gaggtcatgg aagactgggt tagcgacctt gagaagcgat
    11401 ttgacccatc aggcgaacaa cggggaatga gcacagggat cccatcgctg gacaggatgc
    11461 tgtcaccgaa aggtctggtg aaaggctctc tgtttgtcat tggcgctcga cctaagatgg
    11521 ggaaaacgac gctatacagc cagatggcaa tcaactgcgc agtgcatgag aaaaagcccg
    11581 ctcttatgtt cagccttgaa atgccaggtg accagatact ggaaaaactg gtagggcaga
    11641 agtctggtgt taacccgaat attttttacc ttccggcgac aaatgacgct gatgacggct
    11701 atcagggtga ttacgatggt gacttcaaca gggcgatcga aacagccaat cgcttgagtg
    11761 aaatcgacct gctttacatc gacgacacgc cgggattatc tctggctcaa atcgtcagcg
    11821 aaagccgtcg aatcaagcga gaaaaaggat gtgttggcat gattctggtc gattacctga
    11881 cactaatgac cgctgaaaag gccgatcgca acgaccttgc ttacggcatg atcaccaaag
    11941 gactgaagaa ccttgccaaa gagcttgatt gcgttgttgt gcttctgaca cagcttaacc
    12001 gcgcactgga aagccgaacc aataaacgcc cattaccaag tgactcacga gatacagggc
    12061 agattgaaca ggattgcgat tattgggtgg ggatccatcg tgaaggtgct tttgatgaca
    12121 gtgttccacc tagtgaaacc gaactaatcc ttcgtctcaa tcgtcatggc aataccggca
    12181 cggtgtattg cattcaggca aatggcgcta tttatgacac agaccaacag tctgctgaaa
    12241 tgcgccgacg tgaacgcgag gaaccgcagt ccaagaagaa aggaggattc tgatgaccat
    12301 ctacatcact gagctaataa caggtctgct ggtaatcgca ggccttttta tttgggggag
    12361 agggaagtca tgaaaaagct aacctttgaa attcgatctc cagcacatca gcaaaacgct
    12421 attcacgcag tacagcaaat ccttccagac ccaaccaaac caatcgtagt aaccattcag
    12481 gaacgcaacc gcagcttaga ccagaatcga aagctttggg cttgccttgg tgacgtctct
    12541 cgtcaggttg aatggcatgg tcgctggctg gatgcagaaa gctggaagtg tgtgtttacc
    12601 gcagccttaa agcagcagga cgttgttcct aaccttgccg ggaatggctt tgtggtaata
    12661 ggccagtcaa ccagcaggat gcgagtaagc gaatttgcgg agctattaga gcttatacag
    12721 gcattcggta cagaacgtgg cgttaagtgg tcagacgaag cgcgactggc tctggagtgg
    12781 aaagcaagat ggggagacag ggcggcatga ggcgacagcg acgaagtttc accgacatca
    12841 tctgcgaaaa ctgcaaatac cttccaacga aacgctccag aaataaacgc aagccaatcc
    12901 caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat atccggtg
//
LOCUS       DADLTI010000115        12876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLTI010000115 DADLTI010000000
VERSION     DADLTI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.115"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JN755_002975"
     CDS             complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JN755_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7381705.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JN755_002976"
     CDS             complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JN755_002976"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7381706.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2576..2854
                     /gene="ychH"
                     /locus_tag="JN755_002977"
     CDS             2576..2854
                     /gene="ychH"
                     /locus_tag="JN755_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7381707.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JN755_002978"
     CDS             complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JN755_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7381708.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4713..5660)
                     /locus_tag="JN755_002979"
     CDS             complement(4713..5660)
                     /locus_tag="JN755_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7381709.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JN755_002980"
     CDS             complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JN755_002980"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7381710.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JN755_002981"
     CDS             complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JN755_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7381711.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7499..8755
                     /gene="hemA"
                     /locus_tag="JN755_002982"
     CDS             7499..8755
                     /gene="hemA"
                     /locus_tag="JN755_002982"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7381712.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8797..9879
                     /gene="prfA"
                     /locus_tag="JN755_002983"
     CDS             8797..9879
                     /gene="prfA"
                     /locus_tag="JN755_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7381713.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9879..10712
                     /gene="prmC"
                     /locus_tag="JN755_002984"
     CDS             9879..10712
                     /gene="prmC"
                     /locus_tag="JN755_002984"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7381714.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10709..11101
                     /locus_tag="JN755_002985"
     CDS             10709..11101
                     /locus_tag="JN755_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7381715.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11105..11914
                     /locus_tag="JN755_002986"
                     /pseudo
     CDS             11105..11914
                     /locus_tag="JN755_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            11950..12804
                     /gene="kdsA"
                     /locus_tag="JN755_002987"
     CDS             11950..12804
                     /gene="kdsA"
                     /locus_tag="JN755_002987"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7381716.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3227 a         3230 c         3330 g         3089 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1501 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1561 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1621 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1681 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1741 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1801 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1861 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1921 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     1981 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2041 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2101 tgtagtcggg actaacaggc ggacatcttc gcctccaagg gtgactcgcg aagtataacc
     2161 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2221 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2281 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2341 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2401 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2461 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2521 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2581 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2641 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2701 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2761 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2821 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2881 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     2941 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3001 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3061 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3121 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3181 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3241 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3301 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3361 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3421 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3481 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3541 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3601 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3661 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3721 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3781 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3841 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3901 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     3961 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4021 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4081 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4141 tggtaccgat agtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4201 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4261 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4321 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4381 tccccgcaac agctgcggta tataaaccgt actggggtgc cacaccacta ccaatagcca
     4441 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4501 taaaccgtgc ggcagtatat ttttctttcc agcaagcgtc gatcagagcg cggaaaggca
     4561 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4621 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4681 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4741 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4801 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4861 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4921 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     4981 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5041 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5101 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5161 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5221 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5281 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5341 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5401 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5461 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5521 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5581 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5641 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5701 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5761 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5821 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5881 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     5941 cctgtcccag tcaggcgcga cggggcgtat tttaacagcc aggaaagcac cgcatcaacc
     6001 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6061 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6121 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6181 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6241 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6301 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6361 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6421 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6481 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6541 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6601 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6661 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6721 ccatattggt tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6781 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6841 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6901 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     6961 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7021 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7081 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7141 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7201 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7261 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7321 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7381 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7441 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7501 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7561 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7621 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7681 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7741 agaagatctg cgtaaaaacc tctactggca tcaggataac gacgcggtta gccatttaat
     7801 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7861 taaaaaagcg tttgccgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7921 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     7981 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8041 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8101 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8161 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8221 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8281 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8341 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8401 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8461 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8521 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8581 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8641 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8701 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8761 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8821 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8881 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     8941 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9001 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9061 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9121 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9181 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9241 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9301 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9361 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9421 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9481 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9541 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9601 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9661 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9721 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9781 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9841 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9901 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
     9961 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10021 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10081 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tctccagcga
    10141 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10201 aacaaccttg ccgtattctc gatctcggga cggggaccgg ggcgattgcg cttgcgctgg
    10261 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10321 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10381 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10441 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10501 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10561 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10621 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10681 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10741 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10801 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10861 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10921 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    10981 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11041 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11101 agttatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11161 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11221 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11281 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11341 tcgtctttcc gatgcattat ggctggacta ggtgttaaag aatcgacagg gcagtgcggt
    11401 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11461 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11521 taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt taaagggaaa
    11581 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11641 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11701 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11761 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11821 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11881 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    11941 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12001 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12061 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12121 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12181 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12241 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12301 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12361 aagaaaccac agtttgtcag cccggggcag atgggtaata tcgttgataa attcaaagaa
    12421 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12481 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12541 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12601 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12661 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12721 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12781 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12841 cgttaagact tttttcatgg gtgccagata caaaaa
//
LOCUS       DADLTI010000116        12786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLTI010000116 DADLTI010000000
VERSION     DADLTI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.116"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN755_002988"
     CDS             complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN755_002988"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7381717.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN755_002989"
     CDS             complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN755_002989"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7381718.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(778..1512)
                     /locus_tag="JN755_002990"
     CDS             complement(778..1512)
                     /locus_tag="JN755_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7381719.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN755_002991"
     CDS             complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN755_002991"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7381720.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3638..4615
                     /gene="epmA"
                     /locus_tag="JN755_002992"
     CDS             3638..4615
                     /gene="epmA"
                     /locus_tag="JN755_002992"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7381721.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            4834..6336
                     /gene="yjeM"
                     /locus_tag="JN755_002993"
     CDS             4834..6336
                     /gene="yjeM"
                     /locus_tag="JN755_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7381722.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN755_002994"
     CDS             complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN755_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7381723.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN755_002995"
     CDS             complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN755_002995"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7381724.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN755_002996"
     CDS             complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN755_002996"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7381725.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            12032..12577
                     /gene="orn"
                     /locus_tag="JN755_002997"
     CDS             12032..12577
                     /gene="orn"
                     /locus_tag="JN755_002997"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7381726.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         2904 a         3334 c         3394 g         3154 t
ORIGIN      
        1 gttagattgt aacgacacca atcagcgtga caactgtcag gatagcagcc agaccgtaga
       61 aaacccattt gcccgcaggc acgtggattt tcagatcgtg catcgcgtgg tgcatacggt
      121 gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga ccaatgaagc
      181 tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac agccccagtg
      241 gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc cacataccac
      301 cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt ggattaatca
      361 tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc aaccacagtt
      421 accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt tacaatgata
      481 ttggccgctt tcggtgccag ttcaaaccag gttttggtgt gcagcagagc tgccgtcaga
      541 gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa tcccgcccag
      601 gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct gaaccacaca
      661 gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa ttttttccac
      721 caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt gcactcctta
      781 gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc cctgctgaat
      841 ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca cgaaagtaca
      901 gctccataca ccgttctggc tgttcaactg cgccatacgc tccttcttac cgtggtcgcg
      961 gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga actctgggtt
     1021 caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac cggagaactg
     1081 gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg cggtgcggga
     1141 gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg tcatatcgac
     1201 caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac cgtcggtgta
     1261 atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca tgccgcagga
     1321 accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca ggttgtcttt
     1381 gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt catagaatgc
     1441 gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt tcaggttttt
     1501 catctcagcc attcgccttc tccttattat tggctgcttc cgccttatcg gctgcatccg
     1561 cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg tcgctgtact
     1621 ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgaga aagttgacgt
     1681 cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac tctttacgtg
     1741 ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggctcagt tcaatggtgt
     1801 agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc ttgaagcgtt
     1861 cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta cggtagatac
     1921 cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag ttttcgccgc
     1981 catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc gcttcaattg
     2041 ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca ccggccagac
     2101 ggccgaagac caccagttcc gccagggagt tggagcccag acggtttgca ccgtgcagac
     2161 caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca cagttctgat
     2221 cggtttcgat accgcccatg gtgtagtgtg cagtcggacg taccggaatc ggttctttaa
     2281 ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga cgttcatgca
     2341 gttttttctc gccgaggtga cgcaggtcga gataaaccac atcaccacgc ggcgtggaga
     2401 tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg cgtggaccca
     2461 gttccatata tttgtttttc ggctcgccca gcggagtttc cgggcccatg ccgtaatctt
     2521 gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccacgg cagccttcgg
     2581 tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat tccatgtcac
     2641 gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg atgccgccgt
     2701 tggtgttgta acgataaacg cgacccgcac cgccggtagc cataacgacc gcgttagcac
     2761 ggatctgcac caacgtgcct tccatcatgt tcattgctac caggccgcga acatgaccat
     2821 catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc gggaattgca
     2881 gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg gcgaaccagg
     2941 tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc gggcgacggc
     3001 tccatgggca tccccacagt tccagttggg tcatttcggt tgggcagtgg tggacgaaat
     3061 aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg tgaaagtgat
     3121 attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca gcaacggtat
     3181 ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcatttgga tttgcctgcg
     3241 cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca agatcggctt
     3301 gaaaggtttg cacgacattc ctccagattg tttttatccc acagccccgt acttcagggt
     3361 aagtgcctga aagttacggt ctgcgaacgc tattccactg ctcctttata ggtacaacag
     3421 tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg cgcaagaggg
     3481 ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt gcttttttta
     3541 gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg cgttgcgagt
     3601 agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg catcctggca
     3661 gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg agatccgtcg
     3721 tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc aggcgacggt
     3781 aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc attcgcaggg
     3841 gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgcctgc tggttgctgg
     3901 ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg ggcgttatca
     3961 caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt accggttgat
     4021 gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag aaagcctttc
     4081 ttatcaacaa gctttcttgc gttatctgga aattgacccg ctctctgccg acaaaacgca
     4141 actgcgggaa gtcgcagcga aactggattt gagcaatgtt gctgataccg aagaagaccg
     4201 cgacacgttg ctacaattgc tgtttacctt tggcgtagag ccaaatattg gtaaagaaaa
     4261 accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa tcagtaccga
     4321 agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc tggcgaatgg
     4381 tttccatgaa ttaacggatg cccgtgagca gcaacaacgc tttgaacaag ataaccgtaa
     4441 gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg aagccttgaa
     4501 agtcggtatg cctgactgct ccggcgtggc attaggtgtt gatcgtctgg tgatgttggc
     4561 gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg cataattctg
     4621 aaaaattact gaattaacag cgaagaatgg cgtgataagc gccattctgt agcatatgtt
     4681 ttcatttttc cgttaaaggc cacaatttaa gactggatat ttgatatcat caaggtatca
     4741 attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc agtgggcacg
     4801 tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa aaagatgagt
     4861 ctgataggac tcatattgat gatctttact tccgtatttg gatttgccaa tagcccatcg
     4921 gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc attgttattc
     4981 tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa agaagaaggt
     5041 ggtatctatt cctggatgaa taatagtgtc ggaccacgtt ttgccttcat tggtacgttt
     5101 atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt ttgggtaccg
     5161 ttctcaacat tcctctatgg tagtgacatg acccagcact ggcgtattgc cggactggag
     5221 cctacgcagg tggttggtct gctggcagta gcatggatga ttctggtcac cgtcgttgct
     5281 tcaaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc agtaatgtgt
     5341 ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg tgggcatttc
     5401 gcgcaggata ttaatttcct tgcatcaccg aacccgggtt atcagtccgg tctggcaatg
     5461 ctatcgtttg tggtatttgc tatttttgcc tatggcggaa ttgaagcggt tggtggtctg
     5521 gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt tgccgctatt
     5581 gttatttcaa tcggttattc gctggcaata tttttatggg gcgtcagcac aaactggcag
     5641 caggtattaa gtaatggttc cgttaacctt ggcaatatta cctatgtgct gatgaagagc
     5701 ctcggggtga cgctgggtaa cgcactgcat ttgtcacctg aagcgtcatt gtcgctgggc
     5761 gtatggtttg cgcgtattac cggactttcg atgttcctcg cttataccgg tgcgttcttt
     5821 acgctttgct attcaccgct gaaagccatc atccagggga cgccgaaagc gttgtggccg
     5881 gaaccgatga cgcgcctgaa tgcgatgggg atgccgtcta tcgccatgtg gatgcagtgc
     5941 gggttggtta ctgtcttcat tctgctggtt tcgtttggtg gcggtaccgc atcggcgttc
     6001 tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttacct gttcctcgcg
     6061 ctggctttcc cgttttttaa agcacgtcag gatctcgaca gaccatttgt gattttcaaa
     6121 acgcgtatgt cggcaatgat tgcgacggtg gttgtcgttc tggtggtgac atttgcgaac
     6181 gtcttcacca ttattcagcc tgtggttgaa gccggagact gggacagcac attgtggatg
     6241 attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa ctattgcagt
     6301 cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct gcaactcatc
     6361 gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct ccagggccgg
     6421 tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat ttggcagctt
     6481 catgcattac aaacttcccg cctgacgacc tttgcccgca gacgtcagcg ttctccccgt
     6541 ttgtttaccg ttgaggcttt ccagacgcat ctggaagggc gggaatggca tatcgatacc
     6601 gtgggcgtgg aagcctgcca gaatcagctg gtggatctca tggcgtagcg gcatacggtg
     6661 acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccctgtt gcagatccac
     6721 caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg ctgcggtgag
     6781 caggatttcc gtcacttctt cactgttggc atcggcaggg gccggaatcg tcaacactac
     6841 gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa acgccttgtt
     6901 cggcacgatt atctctttac ggtcccagtc gctgatggtg gtggcgcggg tgttaatttt
     6961 tgtcacgcta ccagtaagat cgcggatcgt taccgtatcg ccaatgcgaa tcggtttttc
     7021 gaacaggatg atcaggccag agataaagtt ggcgaaaatt tcctgcaaac caaaaccgag
     7081 accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac caatcattga
     7141 gaagccgacc agcccgccaa tcagcatcag cagatatttt gtgatggtgg tgatggcgta
     7201 acccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca gcgcgggcag
     7261 gttgcgcacc agctgcgtgg tgatgataaa taccagaatg gcaatcagca ccgcaccgag
     7321 ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc acagcgaaat
     7381 attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga ccgaaagcag
     7441 ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga tggcatcgag
     7501 atcgacttcg ctttcatcga cttcaattgc tccttccggg ctactgtgat gatgcgcttc
     7561 ctcttcgcca cgcgcacgtt gcgctaacat ctctgcccgg cgatgcttcg cccggtcaaa
     7621 cgccagcctg cgacgctgga tcagcatcca gcggcggata acgtgataaa ccaccagtag
     7681 caggaaccag atggcaatcg aggtttcaag cctcgctaac agtgcctgcg ccgttgccag
     7741 ataacccacc gccgacgcca gaatggcaac cagtggtgcg ccaatcatca tgttccacag
     7801 catatggttg gtaatgttgt cgccgctgcc ttctttgttg agatacaacg gaataccggc
     7861 ttttttcagg cttagggtga ccaccgccag cgcaccgcaa atgaggataa agcaaagccg
     7921 tcccagcgaa ccggaaaatt cgcggtcgtc gaggttatcg aacatcatca gcgccataat
     7981 cagcccgatg ctcatcaggt agtagcgcat cccacgggaa acacgttcgc gcggccagcc
     8041 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggtg gcgcaaatca tcactaccca
     8101 cagcagcggc acggtggccg ttacaccatc gccaatcgcg accgccagcg gataaggcca
     8161 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggtaacggtg acgcgacgag
     8221 aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga tcctgagtca ctttgccgac
     8281 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
     8341 gaccagaatc aacgcgccaa acagcggcaa aattgtctct ttgctggtca gcatcatcac
     8401 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
     8461 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
     8521 gtgcgttgct tcgttcacct ctttcagcgc atcctccagt tgcccgttgg agactttcag
     8581 cttggtcagt tccaatagta gcgtgtcgcc accctgcaac aatgagttca gcaactcacg
     8641 ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg gcggtcagcg gctgaccgtc
     8701 ggcctgatgg atttgccgta gcagcggctg tttattaagt aaatcctcat aacgtaaccg
     8761 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggttttggca tttctggtag
     8821 ccgtgccacc tgcgcccgca gcgcttcgcc gagcaggttg gacgatccca gccattgcga
     8881 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
     8941 ctgttgcgag gcaacgagct ccatccgctg cgcctgttga ttcagcgctg ccgatagttc
     9001 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgccag
     9061 taattcggta ctttccagcg cccgctccgc ctcaagctga cgttggctat tcaactgatt
     9121 acgtaagccc tgcaaatacg catccagttg ctggctctct ttttccgcca gctctgagcg
     9181 taagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
     9241 gagcgcctta agacgtgcag agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
     9301 agtattgccg gtaagcgttc ccaggcggcg ctcgatctca tttaactgac ggcgggcgtc
     9361 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
     9421 ttgctgggcc tgacggcttt tatccagcaa ctggctgctg acctggagaa tttcctgatt
     9481 cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
     9541 ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg ataacttgct gatattgttt
     9601 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagcgcctc
     9661 tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc tccagttcct gagtgatttg
     9721 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
     9781 agtgataatc aggcgcacgt cagcgtttcc tttgatggat tagacctggt cttttttatc
     9841 gtcaaccaat ggcctggcgt cgtgttctgc ttcgatctct tcagcaggaa gtggggcggg
     9901 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
     9961 gacagacagg ctttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
    10021 aacggtggaa ccgagtttaa agcgacccat ttcctggcct ttcagcagtg ccacagaacc
    10081 gtcgttttcc ccggcaggcc aggtccagcg cttgatgata ccttcgcgcg gcggcgtaat
    10141 ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc gctccgacca gaatctgcgc
    10201 cattgggcca aattcggtat cgaaaaggca aatcacgcgt tcgttacggg caaacagatt
    10261 cggcacgttc tgagcggtga gatggttaac ggaaaagaga tcgcccggca cgtagatcat
    10321 ctcacgcaga ataccgttgc acggcatgtg tacgcgatgg tagtcacgcg gggagaggta
    10381 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc agatagttgc ctgccagcag
    10441 ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
    10501 caactggctg ataacgccat cggcaggcat gaccagtaca ttcggatcgg tatcgattgg
    10561 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
    10621 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
    10681 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
    10741 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaagg tagcctccag
    10801 gccattgttt tgtcgttcct gatccggcca acatgccgga tcctgaaaaa aaggggacga
    10861 ttctaacgac ggttagctta attgtcagcc atccgtatca gaaaagtttt tacgcgtttt
    10921 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
    10981 cccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
    11041 atatttacac agacctaaat agtcatggaa ttcgacaaag ccctgagtga tttgttccgg
    11101 ctccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
    11161 cgggaagtga tacagccgag cggcggtcgt ggtgtgctga ccgagccccg agttgtcaga
    11221 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
    11281 accgacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
    11341 tttcagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tgttgcggta
    11401 gatatccatc tgctcgttga cgaacgccat gccttcgtcg tccagcaggt ctatcttgtt
    11461 gagcacgata atcggctcaa tctgcaaggt ttcgcaggcc accaggtaac ggtcgataat
    11521 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
    11581 tatgggtttc acgccgtcgt agaaatccgg acgcgttaac accgaggtgc gctcatgcac
    11641 cgcttccacg atccctttga tatttacgcc ttccgccgcc ggtttacccg gacgccagac
    11701 tacgcggtcg ccggttacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
    11761 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
    11821 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
    11881 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
    11941 tactgactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
    12001 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
    12061 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
    12121 gctggtgacc gatgccaatc tgaatattct ggcagaaggg ccgaccattg cagtacatca
    12181 gtccgatgaa cagctggcgc tgatggatga ctggaacgtg cgcacccata ccgccagcgg
    12241 gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg gaagctgaac tggcaacgct
    12301 cgaattctta aaacagtggg tgcctgcggg aaaatcgccg atttgcggta acagcatcgg
    12361 acaggaccgt cgtttcctgt ttaaatacat gccggagctg gaagtctact tccactaccg
    12421 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattctgga
    12481 tggttttacc aagcagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
    12541 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttcctggt cacgtaagcg
    12601 ccggaatcca cttgcggcgg cgtgattttg acgctaaatc gaacgtcttg ctgataattt
    12661 cagcacttga gataaaaacg caaaaaaaac ttttttgggg ggttgcagag ggaaagattt
    12721 ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
    12781 gtagta
//
LOCUS       DADLTI010000117        12506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLTI010000117 DADLTI010000000
VERSION     DADLTI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.117"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(67..2016)
                     /locus_tag="JN755_002998"
     CDS             complement(67..2016)
                     /locus_tag="JN755_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7381727.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JN755_002999"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JN755_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JN755_003000"
     CDS             complement(2437..2793)
                     /locus_tag="JN755_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381728.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JN755_003001"
     CDS             complement(2793..4289)
                     /locus_tag="JN755_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7381729.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JN755_003002"
     CDS             complement(4286..4447)
                     /locus_tag="JN755_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7381730.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5020)
                     /locus_tag="JN755_003003"
     CDS             complement(4457..5020)
                     /locus_tag="JN755_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381731.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5017..5541)
                     /locus_tag="JN755_003004"
     CDS             complement(5017..5541)
                     /locus_tag="JN755_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381732.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5507..5923)
                     /locus_tag="JN755_003005"
     CDS             complement(5507..5923)
                     /locus_tag="JN755_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7381733.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5920..6243)
                     /locus_tag="JN755_003006"
     CDS             complement(5920..6243)
                     /locus_tag="JN755_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7381734.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6288..8018)
                     /locus_tag="JN755_003007"
     CDS             complement(6288..8018)
                     /locus_tag="JN755_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7381735.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLSGSDYKKLVNLGGTTSGWVG
                     ETEARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7996..9237)
                     /locus_tag="JN755_003008"
     CDS             complement(7996..9237)
                     /locus_tag="JN755_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7381736.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9237..9419)
                     /locus_tag="JN755_003009"
     CDS             complement(9237..9419)
                     /locus_tag="JN755_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381737.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9431..11188)
                     /locus_tag="JN755_003010"
     CDS             complement(9431..11188)
                     /locus_tag="JN755_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7381738.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11188..11670)
                     /locus_tag="JN755_003011"
     CDS             complement(11188..11670)
                     /locus_tag="JN755_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7381739.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11818..12168)
                     /locus_tag="JN755_003012"
     CDS             complement(11818..12168)
                     /locus_tag="JN755_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7381740.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12211..>12506)
                     /locus_tag="JN755_003013"
     CDS             complement(12211..>12506)
                     /locus_tag="JN755_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7381741.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2821 a         3714 c         2898 g         3073 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agccatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacctggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatgtgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcagggcatt cacgtcctct gcctgtcgcg tgtcttcttc cgtgatttca tacttcgccg
     4621 taaaatcaaa ctggtaatac agttcgtaac ggttcagatc cagcagggtg ccaccgacat
     4681 aaacaatctc gcccccttgc gggtccggca tccatccaag cagggctttc cagagttctc
     4741 tccggacatc atgaacggcg tcataggctg cccactgccc tttttcatca cgctcattgc
     4801 tgagcaccac aatcacggag aagccttcag tcaaatcctg ccagtaatcg gtctgtgatt
     4861 tttgttcccc cggcgcatcg tcagagggaa ccacataggc agcaggcagg cgaagttttc
     4921 cggcatcagg gatcgcctta aactgcgccg cgccaccaac acgctcctca aaacgcgggc
     4981 atcgctcacg cagcgccgca attatcgttg tcagtttcat ttatgcttcc tttttaccgg
     5041 acgtaacgaa cgctgcagct cactggacaa cagttcctgc gtccagtgac gctgacgctc
     5101 aataacgtca gccataaagt tattacgcgg ggccagccgg aaagtcgaag aatggtgctt
     5161 cttctgccgc ttatcctttt tatccattcc atacgctgaa tggcgaacgc cgtaatacag
     5221 aaacgccgga taataaggag caccttcagg aaaacggcga ttcccctgcc cgtttttctg
     5281 gtcaggggaa attttcacca tcagtccggg gcgacgcgtc gtttttttgg gaacgtaata
     5341 accaatggag cgggccagac gcccggtctg atatcccggg ttctctcccg gaccggagcg
     5401 tccgcgttta atcaccagac gtctggcatc acgcatgtaa acgcgcccga tttgcacaaa
     5461 cgcccgacgc agacgggcgc gattaaactc cagctcctct ggttgtttaa agtcgacgtg
     5521 taaaaatgct gtctgattca tggcattcac cccgtcgtcg cgctgtacgc agttcttcac
     5581 attccagtaa taaaaaacgc cgctgaccgt tcaggtcacg tattcgccgg atccggtact
     5641 cttggccgta ataaaccact tcgtgatctg ccgtgatgtc gtgccggaaa cggatcgtaa
     5701 aataatgcgt aacgatattt tctgtctgca ctgagccctg ataagcggca gcgcctggct
     5761 gagccacctt tgcccagaca tcaaacgact ccggatacgt tggctccgta ccaaaatcag
     5821 cggtgggttc atccacacga aggcggatct ttatccggcg attcagctct ccgggatccg
     5881 gtaaaaggta agtggcactg gtctgacttt gcctgatttt catagcggga caatcctgta
     5941 agggccagca agccatctga aactcattgg cgtttccagt ttctccacat cggtgatcgt
     6001 tgagcgattt tcataaaaat gactgaccag catcagcatt gccagacgaa cgtcatcagt
     6061 cagatgcatc ccgtcaggat catcttccgg aatcgtctct tccggtgcat acaacttcct
     6121 gttcaggtat gtctctgttt ttttctgtac cgcctgtgcc agcagtttca gaaaatcgcc
     6181 gtcactgtac aaaccatcat cgagccggag atgagattta atctcctctt ctttcaggag
     6241 catattttcc tcctgtgccc gcgattacgc gggcacaaaa aaccgcatta cgcagcggct
     6301 ttctggcggg ttgcagcccc aattttcatc agcttaatcg cctgagaatc caccagcata
     6361 ccgccggttc gcttggtggt ataaaaaccc acgaacggtt tgttggtgta cgggtcacgc
     6421 aggatacggg taccgatgcg atcaacgatg gtatagccgc gtttgaagtt cccaaacgca
     6481 atggcttttg catcggcggc aatatccggc atctgctcat tctcaacgat gccataccct
     6541 gccagagaag aaggctgacc cagctcaata cccggacgcc acaaataatt tccgtcgtta
     6601 tccttcagca gacgaatggc aaacaggctg ctgttgttca tcatgaactt cgcgccgctg
     6661 cggtgcgcct tacgcaaggt gtaaatcagt ttaatgatcg catcagcggt cacgccggaa
     6721 gccgcgccgg aagcaatgtg ctgaagtttg ccaaacgcac gggtcttgtc atcttcatcg
     6781 gtggactcat aagccagaaa accttttggt tttttgctac cgtcgccact ggtaaaggca
     6841 atttcttcct gttcggcaaa ttccagcgcc agctcactgt tgatccagtc ttccacatta
     6901 aagaaagcat catcgagcat tttctgtgtg gcctgcgggt tgccgtagat ttcccccata
     6961 aagggctcaa tcagtcccag ttttgaggtg gcggtttccg gacgcgcttc cgtttccccc
     7021 acccatccgg acgtcgtgcc gcccagattc accagttttt tataatccga gccactcaga
     7081 gtgattacgg tggcctcctg gcgcatcacc acttcatctt tcagcaacgt cagaatggtg
     7141 cgatccagtt cttccggaat ggcataacca ccatcttcat cattacccac ctgcagtgcc
     7201 tttcgctcaa gctcacgcag gccatcttca cgccccttgc gcataaaacc gataaacgct
     7261 tctttatgtt ccccggcaac tttattttgc gtaccacctg ccggacgctt aacttcagcc
     7321 agctcagcct caagatcgct tttgagattt tccagttcag tcagttttcc gttgagggtt
     7381 tccacttctc cgttcagtgc agagcccaca tcctcagcgt cgcgcaggga cagtgcgcca
     7441 tagcccccgg ccatgaatgc tttggcctgg gtacgggaga gtccgacatc acgcaggact
     7501 ctttcgattt ttttctgttc ggggatttcc ccgcgggcca gtgcgttctt gacgtcgctg
     7561 atccgcgcct cgtcgttaga cggaaacgtc accagactga cttcccagag gtcgatttct
     7621 ttcagcagaa aggcttcttt gctccggtcg tattcccagt ctttcaggac gtacccaata
     7681 gaaaggccgg ttaacgaacc ggccttcatg tgtgcatgtg cacgttttgc gaggggatca
     7741 tcatcaataa gcaaccgtcc cctgacgtaa agcccgacat cgtcttcctt catttcggtg
     7801 taaacaccga tgggttcatc catgcggtgc tgccagagca gcgcaggtaa cgcttttctg
     7861 tcactccacg cccgcaggga agcagcaaat gccccggaca tcaccacatc atcgtggctg
     7921 tcctttacac caaagacgga gccatgccct tcaaactcac cggagtcact gacagatttc
     7981 agactcagcg gtacatcaag acgttgtttc gtctgcattg gcgttatcct tctgcttacc
     8041 ggctttactg ccatcggagg gtttcgtggt catgttcatc ggtgtgagat agacatcccc
     8101 accgggacgc ggattcatat cttccaggtc gcggcagtca ttgggagagt aaatccccca
     8161 gttaatcccg gtggcgtagg cttcaaaacg ggatttcata tccccgcgca gtaacgcccc
     8221 ggcgttaaat ttggcgtaaa acttcccctg tttgctctcc ctgaccagcc ctgtattgat
     8281 ccgttgttca atacgggtca gatacggcac aagggaatag ttgataaatc cgagccccag
     8341 ctcttcaata ttgttgaaag tggcgcgatc ggtgttctgc accatgtgca gcggcacgcg
     8401 gaaaagacga cagatttctt ccaactgaaa cttgcgggtt tccaggaact ggctgtcctc
     8461 ggcattcagt gccaccggct tccagtccag ccccatttcc agaatcatcg gacggtgcgc
     8521 attacccagc ccaagatgac gctcctcaaa atccctcttc atgcgctcat aagcatccgg
     8581 cgtgagcttt tgttccgtgc gcaacacacc ggatgtcacc gcaccattac caaacaacct
     8641 ggcaccgtgc tcctcggttg ccgctgccag tgaaatggcc tcgcgcgcat atgcaatggg
     8701 attcagcccg accagtccgt ccagcgtcag ggtgcgcaca tgccagattt catcctgggt
     8761 caacacatcc acggaaccat ccggaaacgt cacctgataa accggctgcc actggctgtt
     8821 cagcttcggt tccacacagc ccggatctat cggaagaagc tccacaactt cccccagcgc
     8881 cttcaccttg taagcgtaaa aattcccccg cagacacagg cagacgataa ccagctccca
     8941 gaattcctgc ggcgtcatgt agccattggg ttttgctgaa atcagcttat gcagccgttc
     9001 atccaccgcc cgtgttttaa gggtgccgct gattttgtag agactgcagg gcagcatacc
     9061 aacagactca gccagcaccc tgacgcagga atagaccgcc gtcagccgca tggcccgctg
     9121 gctgctgatc cgctttccgg tataggtgtc gtatgacaac cccagctctt ccgcaagcat
     9181 cccgggcgtt gtgacggggg tgttattttt gcgttgaaaa agcccctgga aaaacattac
     9241 tcacctccgg aggcgacccg gtgaccgcga tcgagataac gcgcaaccag ccacgaccag
     9301 cacagacaca gcgccccggc aacaacaaaa cctgccgggg gataaatcag ccatgtgcca
     9361 tacgacagca aaagcgcccc cagcacgccc accagtggcg tgagaattat cagaaacata
     9421 atgacctcgg ttaaagcgag cgaataccaa cgctgaccag atgctcagac agatccggct
     9481 ccggttcacc gccattgacc agcatccggc tcattgctgt aaacatcgca acagggccgt
     9541 cgattttggc ttccggcgtg gatttgttcg ggaagatatt gtcgtttttg tccggtttca
     9601 ccgtaacgtt agacatcatc cagttcatga ccgggtgatt actgtgatgg aaacgcccgg
     9661 catagaccag tgattccgtt tccttcatgg cctctgacag attgcggacc gtctgcggaa
     9721 cttccaccag cggtatacct tcttcagcca gcgccagact gaactgcatt gcgctccacg
     9781 ggtcaaatcc cagttccctc aggttttcac cgccaatcca ttccagtaag tcacttttta
     9841 tctgagcatg atcgataaca tcaccatccg tcagaatcag cttatccatc tccgcccact
     9901 tccggtaaag ttctgcctgc tgccgcgagc atcgttccag ccgtccttcc ggaagccaga
     9961 atttaaaatc ggcatgaaca tgcccgttat ccgttcgcca gagttttgcc gccgcacaga
    10021 tatcaatctt atgagcaagg tcaacgccga cccacatggg atacgttttc agctcatgtc
    10081 gtggggcaat gtattcgcac ttctcccact taatcatgtc catccaggca gactctgctg
    10141 ttacccacac attcatgtgt ttggtaaaaa aattcacccg cgcagagacc tgttctttcg
    10201 cttttttcgc caggcgacgc agatcatccc agcgtttaca gatgcccagg ccgggattcg
    10261 ctttctgcca gaccgtttca tcaaacggat catctccctc atcgagggtg taaataatcg
    10321 caaagtagga gtcgtctttt accgcgccct ccacgtcgct gttatagcca cgcaatacct
    10381 tgatggcgta atcacgctgc tcgtaacaaa ttccttcctt gttaaaccct gccgtggtga
    10441 taccaaataa aagggactgc agacgggcac cggttgccgt ttccagaacg tcccacacgt
    10501 cacgggtttt atgtgcatgc agctcatcaa taatggcgca gtggatgttc agaccatcca
    10561 ggttgtttgc atctgaagaa agcggttcaa attttgatgc gctctgctcc tgataaatcg
    10621 ccagcttgtt gaaatcaaac aaccgcccga gtgtcgaccg ggcttttctg accatatttt
    10681 tggcgtcttc aaacacgatt ctggcctggt cacgcgtggt tgcggctgaa tacacctcag
    10741 ccccgccttc accatctgcc cccgtcatat acagaccgat acctgatgac agggttgatt
    10801 ttgcgttttt acgggcgact tcgttgtacg ctgtccggaa acggcgcacc atcaccgggc
    10861 gtccgctgcc atcgctgcgc atgacaactt ccccggtctc ttcattcacc agcggaatga
    10921 caaaaccaaa aatattaatg aggataaaca catgccagtc catcaactca atgggctgac
    10981 ctgccagcgc cccttttaca tggggcacaa atttgtagaa attcaggatg tgctgcgcac
    11041 ggggttcact gaaataaatc ccccgctctt cgccgtactt cagatcatca agaaaacgct
    11101 ggcaggccag acggacaaat tcaccagcga caatttctcc tgcaacaaca cgttcggcgt
    11161 agcggatccc gtcagccact tttgccatca gtctctcgct tttaaaagtt ctgccagcgg
    11221 atcaacatca tccggtccgg cgatatttac tttcgcccgg cttgccggtg acataccaaa
    11281 ttctgcaagc atcgcccgga tccgcttcca ggcatccgct ttcatcgcag cagccgggtg
    11341 tgccttaatc agcacatcac cgttctgcgt ttccgtgcgg taggtataac cctcaacatc
    11401 gagggtttcg cagtgatgcc ggtattcggt gtaggcttcc accagcaact cgagtgcacg
    11461 cgcatcaagc tgagaaatga tcccttccgc attcagctct tccgccattc gcctgaacca
    11521 gtactttccc tgcgccccta aatgctgcgg aattttaggg agaccttttt cgtccttttt
    11581 agcggttttt ttggcgtctt taacgggacg ctttgaggga ttgcctcgta tcaaatgcag
    11641 gcgtggcggg gttttcgggg gtcctgacat aatcgatttt acctatcaat cgcttgatcg
    11701 cattcccaaa aaaaagtttt cgaacctgcg gcgatgcgag gaagggttgg cgggcggtcc
    11761 cggacagcca gggctgcagg gatttgaccc gcccctccct acaagtgaga ataattatca
    11821 cctgattcgt tcgcgcgctg ttttcgcttt atggcagggc cagcacagac tctgcaggtt
    11881 gctgtctgcg tctgttccgc catgcgcttt cgggatgatg tggtcgacgg ttttcgcctc
    11941 gctcaccaca ccgacacgca gacacaactg acacagacct ttatcgcgct tcagaatacg
    12001 ggcacgaatc accgtccatt ttgagccata gccacgctgg tggcggctca gtccgcgctg
    12061 gtgctgcacc cagccttcac cgcgatgctt atcgcagtaa cccgaactgt ctgtggttgt
    12121 acctgcacat ccacgcttac ggcaggcacg tgggattagt gatggcataa atacctcata
    12181 ccctgcgaaa tgtttaccac gataaaaagg ctacttaatg cactgagtgc ggatatactc
    12241 ctgtgcccct tccagttgca tctgcatcgt catcagccgc tctctgaggg tgaaataatc
    12301 ccgttcagcg gtgtctgcca gtcgggggct ggttgcatta tccacgccgg aggcggtggt
    12361 ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac
    12421 atcagcgcgc agagtttcat tttcagcttt cgcatcagct aactccttcg tgtatttagc
    12481 atcgagtgca ttaacatcac gctgtc
//
LOCUS       DADLTI010000118        12427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLTI010000118 DADLTI010000000
VERSION     DADLTI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.118"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN755_003014"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN755_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7381742.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN755_003015"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN755_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7381743.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN755_003016"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN755_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7381744.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN755_003017"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN755_003017"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7381745.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JN755_003018"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JN755_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7381746.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JN755_003019"
     CDS             complement(8217..9110)
                     /locus_tag="JN755_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7381747.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN755_003020"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN755_003020"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7381748.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCSFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JN755_003021"
     CDS             complement(10007..10189)
                     /locus_tag="JN755_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7381749.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JN755_003022"
     CDS             complement(10241..11473)
                     /locus_tag="JN755_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7381750.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN755_003023"
     CDS             complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN755_003023"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7381751.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2868 a         3537 c         3084 g         2938 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gagcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagacc
//
LOCUS       DADLTI010000119        12270 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLTI010000119 DADLTI010000000
VERSION     DADLTI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.119"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..389)
                     /gene="yafO"
                     /locus_tag="JN755_003024"
     CDS             complement(<1..389)
                     /gene="yafO"
                     /locus_tag="JN755_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7381752.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
     gene            complement(392..685)
                     /gene="yafN"
                     /locus_tag="JN755_003025"
     CDS             complement(392..685)
                     /gene="yafN"
                     /locus_tag="JN755_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7381753.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(737..1792)
                     /gene="dinB"
                     /locus_tag="JN755_003026"
     CDS             complement(737..1792)
                     /gene="dinB"
                     /locus_tag="JN755_003026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7381754.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1863..2648)
                     /gene="lafU"
                     /locus_tag="JN755_003027"
     CDS             complement(1863..2648)
                     /gene="lafU"
                     /locus_tag="JN755_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7381755.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2593..4332
                     /gene="flhA"
                     /locus_tag="JN755_003028"
     CDS             2593..4332
                     /gene="flhA"
                     /locus_tag="JN755_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7381756.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            complement(4549..5046)
                     /locus_tag="JN755_003029"
     CDS             complement(4549..5046)
                     /locus_tag="JN755_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7381757.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5222..5835)
                     /locus_tag="JN755_003030"
                     /pseudo
     CDS             complement(5222..5835)
                     /locus_tag="JN755_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6127..6867
                     /locus_tag="JN755_003031"
     CDS             6127..6867
                     /locus_tag="JN755_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7381758.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6838..7605)
                     /locus_tag="JN755_003032"
     CDS             complement(6838..7605)
                     /locus_tag="JN755_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7381759.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7811..8389)
                     /gene="lpcA"
                     /locus_tag="JN755_003033"
     CDS             complement(7811..8389)
                     /gene="lpcA"
                     /locus_tag="JN755_003033"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7381760.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8629..11073
                     /locus_tag="JN755_003034"
     CDS             8629..11073
                     /locus_tag="JN755_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7381761.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11116..11589)
                     /gene="ivy"
                     /locus_tag="JN755_003035"
     CDS             complement(11116..11589)
                     /gene="ivy"
                     /locus_tag="JN755_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7381762.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11743..>12270
                     /gene="yafV"
                     /locus_tag="JN755_003036"
     CDS             11743..>12270
                     /gene="yafV"
                     /locus_tag="JN755_003036"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7381763.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQAL"
BASE COUNT         2894 a         3204 c         3220 g         2952 t
ORIGIN      
        1 atgcgaaacg cttctgccat ttttccaatt ttatgcattt ggttgttatc tcgagccagt
       61 ttatgaggtt caggcttcag aatggcaatg agcaaccatg cttgctcatc aaacgcaccc
      121 tgacaatata ccaaatgcgc ttcgtcattc gttctgctga attggcgcaa ctgtggcgga
      181 aatggattat tcacatttgc cagatgaata tgagcaactc gctcaaattt gattaatggc
      241 caggtaaacg agtcgtcgta gagtgcatcg cgaccaaata tatctggcaa aacaccgtca
      301 cgcttatagg aaataaaatc cgccgttaac gcatcaagtt cctctgctgt aagttgcagg
      361 cgaataagtt ttgttttgaa tacccgcatc cttattcctt aaagtcattg aaatcatcat
      421 ccgtcatatc attaagatat tgtttagcgc ggcgtgtaat ttcctctaat cttgcagcac
      481 ttggacgaaa gcgcgccttt atgggctttt tctcctcttg ttcagcaagc atgtccatta
      541 acgcttcgaa tgcgctggca cttaagagat atcctgcggg gcgattatta gaaagaaccg
      601 caactggttg atcaataaag tatttagctg ggtttttacg taactcagtg atattgaccg
      661 atttttcagc gagaatccga tgcatacagt gataccctca taataatgca caccagaata
      721 tacataatag tatacatcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
      781 acgtcacatg cagccccacc agacgcacac cgcgcccgcc gcggcgttca tcccaggttt
      841 tacgcgcggt ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgggtgg
      901 tctgctgaaa atcgtcgaac tttaatttca ccccctggcg agcaatcagt aaatcaggtt
      961 ttacctttgc cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt
     1021 cagaccagtg atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc
     1081 gttcgctgtt aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa
     1141 atttgccaaa gcgtttaagc agaatcacca gatcacactt ttgtacatca ccgcaggtcc
     1201 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccgggg attttttcca
     1261 gtggtaaggt ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tgaccgttgg
     1321 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagatg
     1381 ccgtcagttg cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag
     1441 aaccgtggca atggacgcta tcggtaacat cgagataagc ctcatccagt gacaacggtt
     1501 caatgcgcga ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg
     1561 cgtcaaagcg ccccggaagc aaggtgagat gtgggcataa tttgagcgcc atcccagtag
     1621 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
     1681 gacgttcgcg gctgccgcca atagcaatag ggatatcgcg cagagcggga ttgtcgcgca
     1741 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc
     1801 tctcaacact ggtaaagtat acagtgattt caggttttga gaaatgcgta aagattcagc
     1861 atttagcgca ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
     1921 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
     1981 agtaccataa tctcaatacg ccgattgccc gcgctttgcg gattcttcgc gtccagcagc
     2041 atctggtccg ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc
     2101 agcacccgac gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg
     2161 tttttgtagg ccatcgcatc ggtatgcccg gtaataataa ttttgttata gagcgagtcg
     2221 aacactggcg cgagctccac cagcaaagtt ttaaagaacg gcataatctg cgcgctgccg
     2281 cgttcgaaca tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca
     2341 atctccatct ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt
     2401 gccaactcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca
     2461 tcacgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
     2521 gacgggctga ttttatttaa cggcgacagc ccaccaccgt taaaaatcga ctgcccgtgc
     2581 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatc
     2641 gtcgtcacca aagaggccga gcgtatttcc gaggtttctg cccgctttac cctggacgcg
     2701 atgcccggca aacagatggc gattgacgcc gatcttaacg ccggattgat caaccaggcg
     2761 caggcgcaaa cccggcgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac
     2821 ggggcatcga agtttgtacg cggagacgcc atcgccggga tgatgattct ggcgatcaac
     2881 ctgagcggcg gcgtctgtat cggcatcttc aaatacaacc tgagcgccga cgccgccttc
     2941 cagcaatacg tgctgatgac catcggcgac ggcctggtgg cgcagatccc ttccctgctg
     3001 ctctccaccg cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat
     3061 gacgtgcgcc accagttgct ggcaagcccg tcggtgctct acaccgccac cgggatcatg
     3121 ttcgtgctgg cggtggtgcc ggggatgccg catctgccgt ttttgctgtt cagcgccctg
     3181 cttggcttta ccggctggcg gatgagcaaa cgcccgcagg cggcggaggc ggaagagaaa
     3241 agcctcgaaa cgctgacccg caccatcact gaaaccagcg aacaacaggt cagttgggaa
     3301 actattccgc tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg
     3361 gacaaagcgc agggcaaccc gctcacccag cggattcgcg gcgttcggca ggtgatttcc
     3421 gacggtaacg gcgtgctgct gccggagatc cgcattcggg aaaacttccg cctcaagccc
     3481 agccagtacg ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat
     3541 aaactgatgg cgctgccctc cagcgaaacc tacggcgaga ttgacggcgt gctggggaac
     3601 gacccggcgt acgggatacc ggtcacctgg atccagcctg cgcagaaagc gaaggctctg
     3661 aatatggggt atcaggtgat cgacagcgcc agtgtgattg ccacccatgt gaacaagatt
     3721 gtgcgcagct atattcctga tttgtttaac tatgatgaca tcacgcagtt gcataaccgt
     3781 ttgtcgtcga tggcaccgcg cctggcggag gatttaagcg cggcgctcaa ttacagccaa
     3841 ttgctgaaag tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc
     3901 atcgccaccg tgctggtcgc cagtagcacg gtgactaaag atcatattct gctggcggcc
     3961 gatgtgcgcc tggcgctgcg gcgcagcatt actcatccgt tcgttcgcaa gcaggagctg
     4021 acggtgtata cgctgaataa tgagctggaa aatctgctga ccaacgtagt gaatcaggcg
     4081 caacaggccg gcaaagtgat gctcgacagc gtgccggttg acccgaatat gctcaaccag
     4141 ttccagagca cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agatccggtg
     4201 ctgctggtgc cgccgcagct acgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg
     4261 gggctgcatg tgctgtcgta taacgaagtg ccggatgagc tggagttgaa gattatgggg
     4321 gcgttgatgt aagaatccgg catcggtgca caattgccgg atgcggcgta aacgccttat
     4381 ccggcctacc gttcaggcac aagtttttag gcatgataag acgcgccagc gtcgcatcag
     4441 gcatctgcgc ataactgccg gatgcggcgt gaacgcctta tccggcctac cgttaaagca
     4501 cgatttgtag gccggataag cgtaagcgca tccggcacca gcgcacagtt ataaaatgat
     4561 acgctccccg gcacttaaat ccgttatgtc tcccgcccaa tcgatgggat ataaccctcg
     4621 cgcgacatcg cgatggaacg ttgagaatgg ccaatcactc acctgcttta cccagccatg
     4681 tttctctgga tttatataaa tataatcaac atgatggcga taatcttttg tattgcgaat
     4741 ggcatgctcc caaaaacgcg gttgccagat atttttcaat ccgcaagcat gggtgaactg
     4801 ctttttaatc tcccgccagc gagaggaaaa atcatcatcg ctttcgggta atgtccatat
     4861 gcaatgcata tgctccggca aaacgaccca ggcgttaatt tcaaaaggtc tgtctcgctt
     4921 aacgttaata atggcgttac ggagcgtctg gaactggatg gtcagaagat ggcttcgacg
     4981 attgcgtaaa tttaccgtga aaaaccatgt tccccccttg atgtaataac gacgatattc
     5041 cgacatagcg actccttttt ctcaacggta agaggaaaag gaaaggccga caatgacgct
     5101 gtgttatatc gggaaagcgg ctcgcaaaaa cgcacagatt tgtaggcatg ataagacgcg
     5161 tcagcgtcgc atcaggcatc cgtgcacaac cgccggatgc ggcgtaaacg ccttatccgg
     5221 cctacaaaat catctcttcc gtcaaaatcc tgcgtgcgcc taaaaaatgg tcctgccaga
     5281 aaggttcggc taaccggctt acccgaatgg tttccccggt gcgcagtgac tcaataaact
     5341 gaccgtcgcc cagatacacc cccatatggt cggcaatctc gcggctgtgg atatggaaaa
     5401 atagcaaatc tccccggcgc aggtcgttat tcgccacaat cgttgcccga cggtagtggt
     5461 acatctcatt agccgtgcgc gggagtttcg cctcaaggat cttgttgtag gcataaaaaa
     5521 ctaacccact acaatcgaag ccttgatcgg gccgcgtgcc gccccagacg taaggcttgc
     5581 caagctgctg ctccaggcgg tgaatcgcca cttcggtgat gttatgcagg tgatcgctgc
     5641 ttaaaaagtg gttgttttcc gccagctcct gccagcgtct gtcactcgcc ttgagcggcg
     5701 caggaaacca ttctggatgc tgttttatct gctcccggtt gtggtggcgc tggcgagtaa
     5761 agggagcaaa acgcataacg aaaacacgcc tggatgcaaa aatctgccga gcaagaacga
     5821 agacatgaaa gacataaacc ggctgaatga aggtgagaat tttaaaagac cggaggagta
     5881 aacgggatta ttaattgctt atttgcatat tactattgag atttcataat attaggaaat
     5941 aaatgataca tgcgcttttc ggactattga aaactgcccc tgtgctaaat ttgtaaaccc
     6001 agcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
     6061 tattccgatt tgggagccat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata
     6121 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt
     6181 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
     6241 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctttac
     6301 gtcaaaatgg gcgagcaata tcaactgctc gacagttata aaatctgtaa atattccggc
     6361 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
     6421 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattcccc
     6481 aatgcttatg accgtgcgca tggttatgaa gggaaatacc taatgatcca cggcgattgt
     6541 gtatccatcg gctgctacgc aatgaccaat cagggtattg atgagatctt ccagttcgtt
     6601 actggtgcgc tggtgtttgg tcagccgagc gtgcaggtga gtatttaccc attccgcatg
     6661 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcagctg
     6721 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggccaat
     6781 ggccgttacg tggtcagcaa gccgttaagc cacgaagtgg tgcagccaca actggcatca
     6841 aactacacgc tccccgaggc aaaataagcg ccattcgcct ggcataattt tttgccaggt
     6901 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
     6961 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
     7021 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtccgaaag
     7081 cagcatgttg aaaacgccct tcgcccgcag ttcatccgcc agtgaggcga tatatttaaa
     7141 tactgccgcc atgttgcccg gcgtgcgcgg gtaacgctgc gttaatttat gcagcagcca
     7201 gcaaaaggct ttttcgctgt cggtttcacc gaccgggcgg aagttgccgg tttccagtga
     7261 tttgtagccc gtcagttgtc cgttatgggc ataagtccag ttacgccccc ataactcgcg
     7321 ggtaaatggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
     7381 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
     7441 gctgggttgt ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
     7501 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
     7561 gatatcggta gggacgttgg cgctcatccc gagcaattcg cacatgggta acctccacaa
     7621 cagcgggagc gcatggaccc cggcgaagat tgatttaaga attttcaggt cggatgcggc
     7681 gtaaacgaat tattcggcct acggttcggg tacagatttg taggcatgat aagacgcgtc
     7741 agcatcgcat caggcatcag cgcacaagtg ccggatgcgg cgtaaacgcc ttatccggcc
     7801 tacgccagac ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt
     7861 taatgtgaat ctcctgaatg cggtcggcat aaccaaagtg cggaacgcga atttcgatat
     7921 ccgccgtgcc agccatttta ccgccgtctt taccggtcag ggtgatcact ttcatcccct
     7981 tctcacgcgc cgctgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc
     8041 ccagcagtac atcgccttcg cgacctaccg cttcaacgta gcgggagaaa atatcattga
     8101 aaccgaaatc attaccgacg caggaaatat gactaacgtc agaaatagca atcgccgggt
     8161 agcccggacg gttttcacgg tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc
     8221 aatgggaacc gccgttgccg caggaaagca ctttgccacc ggctttaaag ctgtctgcta
     8281 acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca
     8341 gcgtttccgc cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct
     8401 tcagcataaa tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac
     8461 gggcaggtgc tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt
     8521 gagctttgtt gtaattatat tgtaaacaca ttgctaaatg tttttacatc cactacaacc
     8581 atatcatcac aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg
     8641 agtattctcg ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt
     8701 atcagcagtc tgattttgct cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg
     8761 gcgtgggtac tggtgcctct gaccattatc ctcgtgccat ttaactttgc gcctatgcgt
     8821 aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg
     8881 cgcactgaga aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag
     8941 ggcaagccgg actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgctgaagag
     9001 caagcgtttc tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatt
     9061 actcatgagc tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgcttc
     9121 ttcgcgatga ttatcaaaaa agagtacggc ggactggagt tctcagctta tgcccagtct
     9181 cgtgtgctgc aaaaactctc tggcgtgagc gggatcctgg cgattaccgt cggcgtgcca
     9241 aactcattag gcccgggcga actgttgcaa cattacggca ctgacgagca gaaagatcac
     9301 tatctgccgc gtctggcgcg tggtcaggag atcccctgct ttgcactgac cagcccggaa
     9361 gcgggttccg atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag
     9421 ggccagcagg tgctggggat gcgtctgacc tggaacaaac gctatattac gctggcaccg
     9481 attgcgaccg tgcttgggct ggcgtttaaa ctctccgacc cggaaaaatt actcggcggt
     9541 gcagaagatt taggcattac ctgtgcgctg atcccaacca ccacgccggg cgtggaaatt
     9601 ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat
     9661 gtcttcgtgc cgatcgatta cataatcggc gggccgaaaa tggccgggca aggctggcgg
     9721 atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc
     9781 ggcgtgaaat cggtagcgct ggcaacgggc gcgtattctc acattcgccg tcagttcaaa
     9841 atctctatcg gtaagatgga agggattgaa gagccgctgg cgcgtattgc cgggaacgct
     9901 tatgtaatgg atgctgcggc atcgctgatt acctacggca ttatgctcgg cgaaaaacct
     9961 gccgtgctgt cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt
    10021 gatacgatgg atattaccgg cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg
    10081 cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc
    10141 agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg
    10201 gaagcggcga agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt
    10261 cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc
    10321 agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc
    10381 aacctcgccc tgctttctga tgtctcgatg gcactgctgg gcggcagcct gaaacgtcgt
    10441 gagcgtatct cggcacgtct gggggatatt ttaagccagc tttacctcgc ctctgccgtt
    10501 ctgaagcgtt atgacgacga aggccgtaat gaagccgatc tgccgctggt gcactggggc
    10561 gtacaggatg cgctgtatca ggcagaacag gcgatggacg atttactgca aaacttcccg
    10621 aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg tcattatctg
    10681 gcaccgtctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccact
    10741 cgttcacgta ttggtcgcgg tcagtacctg acgccaagcg agcataaccc ggttggttta
    10801 ctggaagagg cgttggtgga tgtgattgcc gccgactcga ttcatcagcg gatctgtaaa
    10861 gagctgggta aaaatctgcc atttacccgt ctggatgaac tggcgcacaa cgcgctgacg
    10921 aaggggctga ttgataaaga tgaagccgct attctggtga aagcggaaga aagccgtctg
    10981 cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg
    11041 ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt
    11101 ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt
    11161 caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca
    11221 ggtgagtttc tcttgcgacg ttttctcatc aatagccgag aacagccccg tcatctgatt
    11281 agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc
    11341 gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc
    11401 gcctttcatc acccatgcag gtagcttatg cccttgcacc atctgattaa acgcagcttt
    11461 ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt
    11521 ggcgatgacc agtgcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat
    11581 cctgcccatg ttattaacct cctaaagcct ggcgataaca cgataatttg caaatcagat
    11641 atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg
    11701 tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctag tttgaagatt
    11761 acgcttttgc agcaaccgtt ggtgtgggtg gatggtcctg ccaacctgcg ccattttgat
    11821 cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat gtttaccagc
    11881 ggctttgcca tggaagcggc agcttcgtcg cttgcacaag atgacgtagt gaactggatg
    11941 acagctaagg cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag
    12001 tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat
    12061 aagcgtcatc tgttccgcat ggcagatgag catctgcatt ataaagcggg caatgcgcga
    12121 gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt
    12181 ccagtgtggt cgcgcaatct caacgattat gacctcgccc tgtacgtcgc caactggcca
    12241 gctccgcgct ctctgcactg gcaggcattg
//
LOCUS       DADLTI010000120        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLTI010000120 DADLTI010000000
VERSION     DADLTI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.120"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            127..996
                     /locus_tag="JN755_003037"
     CDS             127..996
                     /locus_tag="JN755_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381764.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="JN755_003038"
     CDS             complement(1199..1552)
                     /locus_tag="JN755_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7381765.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JN755_003039"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JN755_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7381766.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="JN755_003040"
     CDS             complement(3380..3673)
                     /locus_tag="JN755_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7381767.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="JN755_003041"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="JN755_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7381768.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JN755_003042"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JN755_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7381769.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="JN755_003043"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="JN755_003043"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7381770.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /locus_tag="JN755_003044"
     CDS             7588..8889
                     /locus_tag="JN755_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7381771.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="JN755_003045"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="JN755_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7381772.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /locus_tag="JN755_003046"
     CDS             9319..11016
                     /locus_tag="JN755_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7381773.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPVGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="JN755_003047"
     CDS             11053..11628
                     /locus_tag="JN755_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7381774.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11735..11810
                     /locus_tag="JN755_003048"
     tRNA            11735..11810
                     /locus_tag="JN755_003048"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11768..11770,aa:Phe,seq:gaa)
BASE COUNT         2925 a         3137 c         2986 g         3105 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tgccgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgctcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaggcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga ggctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaggctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg attgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg
     7621 gcgattttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgtgc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg tcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact cagacgcatc tcaactttac acaaatcaaa
    10681 acgatagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11761 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc
    11821 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc
    11881 tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DADLTI010000121        11847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLTI010000121 DADLTI010000000
VERSION     DADLTI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.121"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1019
                     /locus_tag="JN755_003049"
     CDS             <1..1019
                     /locus_tag="JN755_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7381775.1"
                     /translation="ELEEKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTK
                     EELLYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQ
                     GIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDID
                     MKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKV
                     QLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQS
                     RHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     WSPK"
     gene            1032..1802
                     /locus_tag="JN755_003050"
     CDS             1032..1802
                     /locus_tag="JN755_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7381776.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1855..2250
                     /locus_tag="JN755_003051"
     CDS             1855..2250
                     /locus_tag="JN755_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381777.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2291..3034
                     /gene="cmoA"
                     /locus_tag="JN755_003052"
     CDS             2291..3034
                     /gene="cmoA"
                     /locus_tag="JN755_003052"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7381778.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3031..4002
                     /gene="cmoB"
                     /locus_tag="JN755_003053"
     CDS             3031..4002
                     /gene="cmoB"
                     /locus_tag="JN755_003053"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7381779.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4167..6596)
                     /locus_tag="JN755_003054"
     CDS             complement(4167..6596)
                     /locus_tag="JN755_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7381780.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6621..7721)
                     /locus_tag="JN755_003055"
     CDS             complement(6621..7721)
                     /locus_tag="JN755_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7381781.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JN755_003056"
     CDS             complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JN755_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7381782.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8869..9435)
                     /locus_tag="JN755_003057"
     CDS             complement(8869..9435)
                     /locus_tag="JN755_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7381783.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9651..11384
                     /gene="argS"
                     /locus_tag="JN755_003058"
     CDS             9651..11384
                     /gene="argS"
                     /locus_tag="JN755_003058"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7381784.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11561..>11847
                     /locus_tag="JN755_003059"
     CDS             11561..>11847
                     /locus_tag="JN755_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY7381785.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         2939 a         3005 c         2866 g         3037 t
ORIGIN      
        1 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
       61 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      121 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      181 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      241 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      301 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      361 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      421 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      481 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
      541 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
      601 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
      661 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
      721 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
      781 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
      841 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc
      901 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
      961 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1021 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1081 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1141 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1201 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1261 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1321 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1381 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1441 gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg
     1501 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     1561 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     1621 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     1681 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     1741 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     1801 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     1861 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     1921 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     1981 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     2041 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     2101 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
     2161 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     2221 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     2281 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     2341 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     2401 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     2461 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     2521 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     2581 ctgccgtcgt catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     2641 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     2701 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     2761 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     2821 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     2881 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     2941 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     3001 actggtggca ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     3061 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     3121 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     3181 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     3241 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     3301 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     3361 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     3421 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     3481 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     3541 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     3601 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     3661 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     3721 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     3781 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     3841 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     3901 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     3961 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     4021 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     4081 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     4141 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     4201 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     4261 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     4321 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     4381 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     4441 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     4501 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     4561 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     4621 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4681 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4741 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4801 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     4861 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     4921 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     4981 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     5041 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     5101 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     5161 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     5221 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     5281 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca
     5341 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     5401 cgcatcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     5461 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     5521 tgctggcgct gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca
     5581 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc
     5641 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     5701 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5761 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5821 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     5881 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5941 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
     6001 gaggcctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
     6061 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
     6121 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
     6181 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
     6241 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
     6301 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     6361 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     6421 gaagaaacaa tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg
     6481 aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc
     6541 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact
     6601 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
     6661 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
     6721 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca
     6781 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6841 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
     6901 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
     6961 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     7021 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     7081 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
     7141 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     7201 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
     7261 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     7321 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
     7381 gccatttcct ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
     7441 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
     7501 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     7561 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     7621 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7681 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
     7741 ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc
     7801 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
     7861 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
     7921 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
     7981 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
     8041 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
     8101 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
     8161 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
     8221 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
     8281 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
     8341 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
     8401 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
     8461 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
     8521 gcagccatta ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc
     8581 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
     8641 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
     8701 atcgtcaccc gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct
     8761 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     8821 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     8881 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     8941 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     9001 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     9061 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     9121 acgtttttcc ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     9181 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     9241 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     9301 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     9361 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     9421 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     9481 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     9541 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     9601 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     9661 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     9721 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     9781 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     9841 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     9901 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     9961 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    10021 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    10081 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    10141 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    10201 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    10261 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    10321 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg
    10381 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
    10441 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    10501 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    10561 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    10621 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    10681 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    10741 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    10801 cgggtggtac agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
    10861 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
    10921 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    10981 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
    11041 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
    11101 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    11161 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    11221 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    11281 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    11341 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    11401 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
    11461 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
    11521 cccaggaaaa cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
    11581 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
    11641 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
    11701 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc
    11761 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
    11821 tactgaacaa cttaacttgc ttaaaaa
//
LOCUS       DADLTI010000122        11786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLTI010000122 DADLTI010000000
VERSION     DADLTI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.122"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            203..538
                     /locus_tag="JN755_003060"
     CDS             203..538
                     /locus_tag="JN755_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7381786.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            631..714
                     /locus_tag="JN755_003061"
     CDS             631..714
                     /locus_tag="JN755_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381787.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            814..1635
                     /locus_tag="JN755_003062"
     CDS             814..1635
                     /locus_tag="JN755_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7381788.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1674..2003)
                     /gene="mdtI"
                     /locus_tag="JN755_003063"
     CDS             complement(1674..2003)
                     /gene="mdtI"
                     /locus_tag="JN755_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7381789.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1990..2355)
                     /gene="mdtJ"
                     /locus_tag="JN755_003064"
     CDS             complement(1990..2355)
                     /gene="mdtJ"
                     /locus_tag="JN755_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7381790.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2766..3800
                     /gene="tqsA"
                     /locus_tag="JN755_003065"
     CDS             2766..3800
                     /gene="tqsA"
                     /locus_tag="JN755_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7381791.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3825..5213)
                     /gene="pntB"
                     /locus_tag="JN755_003066"
     CDS             complement(3825..5213)
                     /gene="pntB"
                     /locus_tag="JN755_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7381792.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5224..6756)
                     /gene="pntA"
                     /locus_tag="JN755_003067"
     CDS             complement(5224..6756)
                     /gene="pntA"
                     /locus_tag="JN755_003067"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7381793.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7280..8224
                     /locus_tag="JN755_003068"
     CDS             7280..8224
                     /locus_tag="JN755_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7381794.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8410..9792
                     /locus_tag="JN755_003069"
     CDS             8410..9792
                     /locus_tag="JN755_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7381795.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9829..10551
                     /gene="folM"
                     /locus_tag="JN755_003070"
     CDS             9829..10551
                     /gene="folM"
                     /locus_tag="JN755_003070"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7381796.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10548..10883)
                     /locus_tag="JN755_003071"
     CDS             complement(10548..10883)
                     /locus_tag="JN755_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7381797.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11012..11755
                     /gene="rstA"
                     /locus_tag="JN755_003072"
     CDS             11012..11755
                     /gene="rstA"
                     /locus_tag="JN755_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7381798.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3013 c         2834 g         2944 t
ORIGIN      
        1 cgctttgtaa gtagtataat agttaacccg atcaagacta ctattattgg tagctaaatt
       61 ttccttaagt cacaatacgt tattatcaac gctgtaattt attcagcgtt tgtacatatc
      121 gttacacgct gaaaccaacc actcacggaa gtctgccatt cccagggata tagttatttc
      181 aacggccccg cagtggggtt aaatgaaaaa acaaattgag ggtatgacaa tgaaaaaagt
      241 attagctctg gttgttgccg ctgctatggg tctgtcttct gccgcctttg ctgcagagac
      301 tgcgaccaca cctgctccga ctgcgacgac caccaaagca gcgccagcga aaactacaca
      361 tcataaaaaa cagcataaag cagcacctgc ccagaaagcg caggcggcta aaaagcatca
      421 taaaaatacg aaagctgaac agaaagcccc tgaacaaaaa gcgcaggcag cgaagaaaca
      481 cgccgggaaa cacggccatc agcaaccggc aaaacctgct gcacaacccg cagcgtaagt
      541 tttcaacagt aatgctggcg cgccccctcg cgcctgaaaa ttacggtgct aagcgggtaa
      601 cgtttagcac cgcctttagc ggagggtagt atggtgggcc gttatcgctt tgagttcatt
      661 cttatcatcc ttattttatg cgcactgatt gccgcccgtt tttatctttc ctgactgtag
      721 ttatctgatt ttactcccac tttcatcccg tcccgtctat agtatttatg agggtttgct
      781 tttaataatc ataattaccc accagagtgt gatatgcgta caaccattgc tgtagtgttg
      841 ggtgcaatta gtttgacgtc tgcttttgtg tttgcagata aaccagacgt tgccaaatcg
      901 gcaaacgatg aggtcagcac cctgtttttt ggtcatgatg atcgtgtgcc agtgaatgac
      961 acgacccaat caccgtggga tgcggttggg caactggaaa cggccagcgg caatttatgt
     1021 acggcgacgc tgattgcacc caatctggca ttaacggcag gacactgttt attgacgccg
     1081 cctaagggta aagcggataa ggcggtagcg ctgcgttttg tgtcaaataa aggtctttgg
     1141 cgctatgaga tccacgacat agaaggccgc gttgatccga cactgggaaa gcggttaaaa
     1201 gcagatgggg atggttggat tgttcctccc gcagctgcac cgtgggactt cggattgatt
     1261 gtgctacgta atcccccttc tggcattaca ccgttgccgt tatttgaggg agataaagcc
     1321 gcgcttactg ccgcattaaa atcggctggt cgtaaagtga ctcaggcggg ataccctgaa
     1381 gatcatctcg atacgttata cagtcatcaa aactgtgaag tgactggctg ggcgcaaacg
     1441 tcggtgatgt cacatcagtg cgataccttg ccgggtgaca gcggttcgcc tctgatgttg
     1501 cataccgatg acggctggca attaattggg gtgcaaagtt cggctcctgc cgcgaaagat
     1561 cgctggcgcg ccgataaccg ggccatttct gttaccggtt ttcgcgacaa gctggatcaa
     1621 ctgtcgcaaa aataatgttc aaacgctgcc cgacagcgcg ggcagcgtct tcatcaggca
     1681 agtttcacca tgatcattcc agccaacagc aagaccaggc caatccagcc tttacgattt
     1741 aaccgttgac caaacaagat ccaacctgcg gctaacgtgg cggcaatacc aaacccgccc
     1801 cacaatgcat aagcgacaga caagtcgatc cctttaacgg cctgagaaag cgcactaaag
     1861 gccgccagca ccgccgccag ggagagcaag ccaaatattt tgcgacgaaa gccgtcagaa
     1921 aatttcaaaa agacgttagc aacgatttcc agcacgattg ccaatgccag ccaggcggcg
     1981 tgaacccatt caaactgcgc catggttcac ctccagttca ggtttacgcg ctttacgggt
     2041 acctgatttg atcaacacta tcccggcgac cagggtggtt aacccggcaa ttttcatcag
     2101 cgataaactt tcgtcgaata acaaaacgct aaacaaggta ataaataaaa taccgatacc
     2161 ttcccacagc gcataagcta cgcctaaggc gattttttta acggcgaaag agagaaatat
     2221 atacgacaga gaaatcatca ccagcattaa aataaagccg ccatttccct cactgacgct
     2281 cgcccatttc attgacagcg taccggtaat ttctgtagca atagccagac ctaataaaat
     2341 ccaataaata tacattgtcc ttctcctgca agagaactat tttaattttc gcttaattca
     2401 gcgaaaccaa agtaaagtgg tcaagccctg cgcatagggc aagcttaggc agaagaaagg
     2461 actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat gaggtacgaa
     2521 gatgggtaaa tgtagaaaat aacgtcctga acaaattgtc cataatatta caattatccg
     2581 cagtgttgct tctcgtcatc gcagatgata attgtcctcg gtagttgaac acgcctgatt
     2641 tgtatcatag cttaagaatt aactcaaaat attttcactt ctttacctga gcggtttgat
     2701 tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga aaaacgggga
     2761 caagcatggc aaagccgatc atcacgctca atggcctaaa aatcgtcatt atgttgggaa
     2821 tgctggtcat tattctctgc ggtatccgtt ttgccgccga gatcatcgtg ccgtttattc
     2881 tcgcattatt tattgctgtt attcttaacc cgctggtgca acatatggtc cgctggcgtg
     2941 tgccgcgtgt actggcagtg tcgattttga tgacaatcat cgtgatggcg atggtgttgc
     3001 tattagctta tctgggttcc gcgctcaacg agttgacgcg gacgttaccg caatatcgca
     3061 actctattat gacgccgctg caagctcttg agccgttgtt gcaacgcgta gggattgacg
     3121 tctcagttga ccaactggcg cattacattg atccgaacgc ggcgatgacg ttgctcacca
     3181 acttattgac gcagttatct aatgccatgt catcaatatt tttattgctg ctgacggtgc
     3241 tgtttatgct gctcgaagtg ccacaattgc ccggaaaatt tcagcaaatg atggcgcgtc
     3301 cggttgaagg gatggcggcg attcaacgtg cgattgacag tgtttctcat tatctggtgc
     3361 tgaaaacagc catcagcatc atcaccggct tggtcgcctg ggcgatgctc gccgcactcg
     3421 atgttcactt cgcttttgtc tggggattgc tggcctttgc gcttaattac atccctaata
     3481 ttggttcagt tctcgcggca atccccccta tcgctcaggt actggtgttt aatggcttct
     3541 acgaagcgtt gctggtgctg gcgggatatc tgctgattaa tctggtcttc ggcaatattc
     3601 tggagccgcg catcatggga cgtgggctgg ggctttcgac attggtggta tttttgtcgt
     3661 tgattttttg gggatggttg ttaggaccgg tgggtatgct gctttccgtg ccgttgacaa
     3721 ttattgtcaa aattgcgctt gaacaaacag cgggaggtca aagcatcgcc gttctgttaa
     3781 gcgatctcaa taaagagtga cggcctccgc agaggccgtc agggttacag agctttcagg
     3841 attgcatcca cgctggcttt ggcgtcacca aacagcatgt gggtgttttc cttgaagaac
     3901 agcgggtttt gcacaccagc atagccagtg ttcatcgaac gtttaaagac aatcacgttc
     3961 tgcgctttcc acacttccag cacaggcata ccagcaatcg gactcttcgg atcatcctgc
     4021 gccgccgggt taaccgtatc gttagcacca atcaccagta cggtatcggt atcagcaaag
     4081 tcatcgttga tctcgtccat ttccagcacg atgtcatacg gtacttttgc ttcagccagc
     4141 aatacgttca tatgtccagg caaacgcccc gcaaccgggt ggataccgaa acgcacatta
     4201 atactacgag cgcggagttt ctcggtaatt tcagcgacag gatattgcgc ctgcgcgact
     4261 gccatgccgt accccggagt aatgatcact gaatgggagt ttttcagcag ttccgctgtc
     4321 tcttctgcgg tgatttcgcg gtgctcaccc acttcctgat catcgccagt agaagagccg
     4381 tcggtgccga aaccacccgc aataacgctg ataaaggaac ggttcatcgc cttacacata
     4441 atgtaagaaa ggatagcccc cgaagaaccg accagcgcac cggtcacaat cagcaggtcg
     4501 ttgctgagca taaagcccgc agccgcagcc gcccagccgg agtacgagtt cagcatcgac
     4561 accaccaccg gcatatctgc accaccgatg gaggcgacta aatgccagcc gaataccagc
     4621 gcaatggcgg tcattatcag caatgccagc acttgcaggc cgacgctgtc cgtgcgaaca
     4681 aatacaatca gcagcaagaa ggaaacgacc agagccgcca ggttcatttt gtgacggttt
     4741 ggcagcatca acggtttaga cgaaatcttg ccacacagtt tgccgaacgc caccaccgag
     4801 cccgtgaacg ttaccgcccc gatgaagata ccgaggaaca cttccgtcag gtgaatattg
     4861 accagaatcg gtgccattcc cgcgtcatga tgcagataac tgttaaagcc aaccagcact
     4921 gccgccagac ccacgaagct gtgcaggatc gccaccagtt ctggcatttc ggtcatttca
     4981 actttcttcg ccagccggat accaattgcc ccaccaatga ccatcgccag caagatccag
     5041 ccaacattac ccgtatccgg tccaaaaatg gttgcgatta acgcaatcgc catcccggcg
     5101 ataccgaagt tgttaccctg gcgagacgtt tcatgtttcg aaagaccggc cagactgaag
     5161 ataaacagga tcgcggcaac aatgtatgca gctgtaacta atcctccaga catatgttac
     5221 cccttaattt ttgcggaaca ttttcagcat gcgctgagtc acggtgaagc caccgaaaat
     5281 attaatgctg gctataagca ccgcgataaa actaaggaag ctaacccagc ctccctggcc
     5341 aatctgcaac agtgctccga caacaataat ccctgaaatc gcgttggtaa ccgacatcaa
     5401 cggtgtatgc agcgcgtgcg atacattcca caccacgtaa taaccgacaa cgcaggccag
     5461 cgcgaaaacg gtgaagtgcc caagaaactc tttcggcgca acgcttgaca gccaaccgaa
     5521 caggatgatt gccagcgcca tcaacgcgta tttacgccac ggtgagcagg cacatttttc
     5581 ctcagttttc acttccggtg ccgctttttg tgccgcctgc ggctgagctg atacctgaat
     5641 cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg gtcacgccgc gaatcaccac
     5701 atcatcaaaa tcaacagtga tattgccgtc tttctctttg cacaacagtt tcagcagatt
     5761 aacgaggtta gtaccgtaaa gctgtgagga ttgcgtcggc agacggcccg gaagatcggt
     5821 ataaccaatc actttgacac cattttccgt actgaagatt tcacccggca cgatgtattc
     5881 acagttgcca ccgttttggg ctgccaggtc gacaatcaca ctgcccgcct tcatggagtc
     5941 aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg cggtggtgac
     6001 aatgatatcg acctcttttg cctgggcggc aaagagttcc atttccgctt tgatgaacgc
     6061 gtccgacatc actttggcat agccatcgcc gctgcccgct tcctcgttaa aatccagctc
     6121 gaggaattcc gcgcccatac tttgaacttg ttctttcact tccgggcggg tgtcgaatgc
     6181 acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccaaacctg caacaccggc
     6241 accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtgattt gcccggtaaa
     6301 gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga tattcgccat
     6361 cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagagt ccatcgccat
     6421 cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg caggccagat
     6481 aaaactcacc agcgttgtcc cgggattcag taacgcaatt tcatcatcta acggcgcatt
     6541 gaccttcaga atgatctctg actgccagac gctattccct tctacaattt cagcgcccgc
     6601 ttgcacaaac gctttatcgt caaaacttgc cagttgaccc gcgccgctct ctaccgcgac
     6661 ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa cacgggtttc
     6721 attggttaac cgttctcttg gtatgccaat tcgcatgata ttcccttcca tcggttttat
     6781 tgatgatggt ttgcctgtgt caggagccac acaagccgct catgtacgag ctaaatgtta
     6841 ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta tgcctgtgat
     6901 ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat tcagccgtga
     6961 tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc gacggtcggg
     7021 taaaacgaga catcgccccg gcacgaatca ctacttaaca ttaaattaac ttatacaatt
     7081 cagttgcttc agtagtaatg atgctgatac ggctgttttt taagcataga cggtcatttg
     7141 agcaggatta aaattggttt aaggaatgtg atatgaaaaa tgacgcagac agttacaccg
     7201 tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc tggtttgcgc
     7261 aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg cactgctttc
     7321 tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag cggcagcggt
     7381 taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg aagcggtgaa
     7441 agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg tcgacacttc
     7501 tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag ccgatgctga
     7561 aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt gtcgaactgc cgaaagatca
     7621 ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc gtagccagcc
     7681 agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa ggtgcctact ctttctacat
     7741 cgttcgtcaa atcgatgcca accagggcgg caaccagcgt attactgcat tcatctataa
     7801 aaaagatgct aagaaacgta tcgtccagag cccggatgtg atcccggcag attccgaagc
     7861 aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga tcccgggtgt
     7921 tgcgactacc gcatcaccaa gttctgaagt cggtcgcttc tttgaaaccc agtcatcaaa
     7981 aggcgggcgt tacaccgtca cgctcccgga tggcactaaa gtcgaagaac tgaacaaagc
     8041 gacagcagcg atgatggtcc cgttcgacag catcaaattc tctggcaact acggcaacat
     8101 gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta agtattacca
     8161 catcacccgc cagtggcaga aacgtggtaa taacctgacc gtcagcgcag atctgtataa
     8221 ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt tttagtcaca
     8281 aatttccccg cgaccattgc atgcagacac atcttttcgt aaaatcccgc gcttcagtgg
     8341 caaactccat ttttatgcgt tttgccctaa tatttattct tcttatcacg ttttaatcac
     8401 tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt attaagctca
     8461 atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc cagcccggca
     8521 gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc ctttgccatg
     8581 ctgatcctca cgcgcattcg tcccgaactt gatggcggta tctttaccta tgcccgcgaa
     8641 gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg cgcggtcatc
     8701 gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac ggacacgccg
     8761 gaattgcgcc tgtttggcga tggtaatacc tggcaatcga tagtcggtgc atcggcttta
     8821 ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag cattaacctg
     8881 gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg ttgtgctggc gatgatgatg
     8941 ttcaaactgg ataccttcaa gctcgacttc accggacttg cacttggcgt acctgtttgg
     9001 gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt ggaaggtgcc
     9061 gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg gcaaagcgac actgctggcg
     9121 gttctctccg ctctgggcgt ttacttgctg gtaacgctgc tttcgctggg tgtggtggca
     9181 cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt ggaaatgatg
     9241 ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg tttccgtttg cggcgcgtac
     9301 ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg ctgccactca taaagcattc
     9361 ccacgcattt ttgcgcgcca ggacgcacaa gctgcgccat ccgcctcact gtggttgacc
     9421 aacatctgcg tgcaaatttg tctggtactc atctggctga ccggttccga ttacaacacg
     9481 ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg cgcgttcttg
     9541 ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg tcggtgcctg catttatggc
     9601 ttatggttat tgtacgcctc tggtccaatg cacttgctgt tatccgttgt tctgtatgca
     9661 ccggggctgc tggttttcct gtatgcgcgc aaaacgcata cacatgataa tgtactgaac
     9721 cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc gacctggatg
     9781 ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat gggtaaaacc
     9841 cagcccttgc caatattaat tactggcgga ggtcgtcgca tcggcctcgc cctcgcatgg
     9901 catttcatta atcaaaagca accggtgatt gtcagctatc ggacacacta tccagccatt
     9961 gatggactga ttaatgcagg tgcgcagtgt attcaggctg atttttcgac caacgacggt
    10021 gtgatggcgt ttgccgatga agtactaaaa accacccatg gtctgcgtgc tattttgcat
    10081 aacgccagtg cgtggattgc ggaaaaaccg ggtgcgccac tggccgacgt actggcttgc
    10141 atgatgcaga tccacgttaa taccccatac ctgctcaacc atgcgctgga aagattactg
    10201 cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt ggtggagcgc
    10261 ggcagcgaca aacatgttgc gtatgctgca agcaaagcgg cactggataa tatgacccgc
    10321 tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgcgcc atcgctgatc
    10381 ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa atcactgatg
    10441 aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact taccagttgc
    10501 tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc atctgcgtta atgcagcttt
    10561 atccagcata taatcaacac ccatgcgctt atcccccaac acgcaacaga accaacaaaa
    10621 gcggcgggca agcgcatcat tccggtgaaa taccacagcg acaccaggta aacaaaatag
    10681 ggaataatcg accacatact aaaaatgatg gttgcgcgta aggcttcaat gccgcgttcg
    10741 ctggcaacaa tataatgcgc gataagcgca aagatcggga aaagtggaat cagcccggcg
    10801 atataataat ttttcgtttt tgctaaaaca ccaatcaaca gcactaccag cgcaccgagc
    10861 gcggctttga ttaccagccc catcttttta ccttaacact tccataacaa gtcatcagta
    10921 gaatacctga tgaaaacttg tttagaaacg attgatagta agtaaaaaca gcgcggtgta
    10981 ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact atcgtatttg tggaagatga
    11041 tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa catgatatgc aggttaccgt
    11101 agaaccgcgc ggcgaccagg ccgaagaaac cattttgcga gaaaatccgg atttggtgtt
    11161 actcgacatc atgctaccag gcaaggacgg catgaccatt tgtcgtgatt tacgcgcaaa
    11221 gtggtctgga ccgattgttc ttctaacctc tctcgatagc gatatgaacc acatcctggc
    11281 actggaaatg ggtgcctgcg actatattct caaaacgacg ccccctgctg ttttgctagc
    11341 gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca ctgaccaaag gtcttcagga
    11401 aacgtctctg actccctaca aagccctgca tttcggcacg ttaactatcg accccatcaa
    11461 ccgcgtagtc accctggcta acactgaaat ctcgctctcg acagctgatt tcgaattatt
    11521 gtgggaacta gctacgcatg ccgggcaaat catggaccgc gatgcattgc tgaaaaattt
    11581 acgcggcgtc agttatgacg gactggatcg tagcgtggac gtggctattt cgcggttaag
    11641 aaaaaaactg ctcgataacg ccgcagaacc ttatcgcatt aaaactgtgc gtaacaaagg
    11701 ctatcttttt gcgcctcatg catgggatag tgacaataaa caatcagatg actaatattt
    11761 gttcatctga aaaagagaac aattaa
//
LOCUS       DADLTI010000123        11710 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLTI010000123 DADLTI010000000
VERSION     DADLTI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.123"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1661
                     /gene="ftsK"
                     /locus_tag="JN755_003073"
     CDS             <1..1661
                     /gene="ftsK"
                     /locus_tag="JN755_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7381799.1"
                     /translation="YQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLH
                     PLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKA
                     DVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLE
                     LPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGS
                     GKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANAL
                     RWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLK
                     KEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIK
                     ANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEV
                     HAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASIS
                     GVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            1816..2427
                     /gene="lolA"
                     /locus_tag="JN755_003074"
     CDS             1816..2427
                     /gene="lolA"
                     /locus_tag="JN755_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7381800.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2438..3781
                     /locus_tag="JN755_003075"
     CDS             2438..3781
                     /locus_tag="JN755_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7381801.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3872..5164
                     /gene="serS"
                     /locus_tag="JN755_003076"
     CDS             3872..5164
                     /gene="serS"
                     /locus_tag="JN755_003076"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7381802.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5404..7848
                     /gene="dmsA"
                     /locus_tag="JN755_003077"
                     /pseudo
     CDS             5404..7848
                     /gene="dmsA"
                     /locus_tag="JN755_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            7859..8476
                     /gene="dmsB"
                     /locus_tag="JN755_003078"
     CDS             7859..8476
                     /gene="dmsB"
                     /locus_tag="JN755_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7381803.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8478..9341
                     /locus_tag="JN755_003079"
     CDS             8478..9341
                     /locus_tag="JN755_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7381804.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9377..10003)
                     /locus_tag="JN755_003080"
     CDS             complement(9377..10003)
                     /locus_tag="JN755_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7381805.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10318..11466
                     /locus_tag="JN755_003081"
     CDS             10318..11466
                     /locus_tag="JN755_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381806.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         2791 a         2842 c         3163 g         2914 t
ORIGIN      
        1 aatatcagca gccgcaacaa ccagttgcgc cgcagccgca gtatcaacag ccgcaacaac
       61 cggttgcacc gcagccgcag tatcagcagc cacaacagcc agttgcgcca cagccgcagg
      121 ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg cacaaaccga
      181 cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg gagccggtag
      241 atacctttgc gcttgaacaa atggcgcgtc tggtggaagc gcgtctggct gatttccgta
      301 ttaaagccga tgtcgttaat tactctccgg ggccagttat cactcgcttt gaacttaacc
      361 tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt gcccgttcac
      421 tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat gtaggtctgg
      481 agttaccaaa taaaaaacga caaaccgttt atctgcgcga agttttggat aacgccaaat
      541 tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc ggtgagccgg
      601 tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc ggttccggta
      661 aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag ccagaagatg
      721 tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa ggcattccgc
      781 acctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg cgctggtgtg
      841 ttaacgagat ggagcgtcgg tataaactga tgtctgcgct gggtgtgcgt aatctggcgg
      901 gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca gacccgtact
      961 ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa ccatacattg
     1021 tcgtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa gtggaagagc
     1081 tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta ctggcaactc
     1141 agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg acccgtatcg
     1201 cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct ggcgcggaat
     1261 cactgctggg tatgggggat atgctctact ctgggccgaa ctccacgttg ccggtacgtg
     1321 tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac tggaaagcgc
     1381 gtggtcgccc acagtatgtt gatggcatca cctccgacag cgaaagcgaa ggtggtgcgg
     1441 gtggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg cagtttgtca
     1501 ctgaaaaacg caaagcgtca atttctggcg tacagcgtca gttccgcatt ggttataacc
     1561 gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa caggggcaca
     1621 acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat tgccggataa
     1681 ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt tcttctttcc
     1741 tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg gagtgacgta
     1801 atttgaggaa taatgatgaa aaaaattgct atcacctgtg cattactctc aagcttagta
     1861 gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga taaagtcagc
     1921 agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa
     1981 ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat gacacaacct
     2041 gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc gttcgttgag
     2101 caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat gctgattgcc
     2161 cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga tgactttgtc
     2221 ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt gggacgtgat
     2281 ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag ttatcagctg
     2341 aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc gccgcaaggc
     2401 gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt cgctcgattt
     2461 ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt tagcacagta
     2521 tatcggtcag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta tcgaagccgg
     2581 gcatttgcat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa ccctcgctga
     2641 agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca cctctggcgt
     2701 gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac cgcaatgcag gtcgccgcac
     2761 tattcttttt gttgacgaag ttcaccgttt caacaaaagc cagcaggatg catttctgcc
     2821 acatattgaa gacggcacca tcacttttat tggcgcaacc actgaaaacc cgtcgtttga
     2881 gcttaattcg gcactgcttt cccgtgcccg tgtttatctg ttgaaatccc tgagtacaga
     2941 ggatattgag caagtactaa ctcaggcgat ggaagacaaa acccgtggct atggtggtca
     3001 ggatattgtt ctgccagatg atacacgacg tgccattgca gaactggtga atggcgacgc
     3061 gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtct atgatagcgg
     3121 taagcgggtc ctgaagcctg aattactgac cgaaatcgcc ggtgaacgta gcgcccgctt
     3181 tgataacaaa ggcgatcgct tttacgatct aatttccgca ctgcataagt cggtacgtgg
     3241 tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt attaccgctg gtggcgatcc
     3301 gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa gacgtcggta atgccgatcc
     3361 acgggcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg gcccggcgga
     3421 aggtgaacgc gccattgctc aggcgattgt ttacctggcc tgcgcgccaa aaagcaacgc
     3481 tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc gaacgcccgg attatgacgt
     3541 gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct acgggcagga
     3601 atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt acttcccgcc
     3661 ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag gcaagattgg
     3721 cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac gctaccgtta
     3781 atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg tggccacatc
     3841 tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc tgcgtaatga
     3901 gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg tagataagct
     3961 gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc tgcaagcgga
     4021 gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag atatcgagcc
     4081 tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag ccgagctaga
     4141 tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc ctgcagatga
     4201 agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc agccgctggg gtaccccgcg
     4261 tgagtttgac tttgaagttc gtgaccacgt gacgctgggt gaaatgcact ctggcctcga
     4321 ctttgcagct gcagttaagc tgactggttc ccgctttgtg gtaatgaaag ggcagattgc
     4381 tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac agcatggcta
     4441 cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta cgggtcagct
     4501 gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag cagacaccag
     4561 taactatgcg ctgatcccaa cggcagaagt tccgctgact aacctggtgc gcggtgaaat
     4621 catcgatgaa gatgatctgc caattaagat gaccgcccac accccatgct tccgttctga
     4681 agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt tcgacaaagt
     4741 tgaaatggtg cagatcgtgc gcccagaaga ctcaatggcg gcgctggaag agatgactgg
     4801 tcatgcagaa aaagtcctgc agttgctggg cctgccgtac cgtaaaatca tcctttgcac
     4861 tggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga tcccggcaca
     4921 gaacacctac cgtgagatct cttcctgctc caacgtttgg gatttccagg cacgtcgtat
     4981 gcaggcacgt tgccgcagca agtcggacaa gaaaacccgt ctggttcata ccctgaacgg
     5041 ttctggtctg gctgttggtc gtacgctggt tgcagtaatg gaaaactatc agcaggctga
     5101 tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg aatatattgg
     5161 ctaataccca atttttctga atctaaaaag cgcctttagg gcgctttttt tgtctccctt
     5221 tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta
     5281 cctatctctt tgattttcaa attattcgat gtatacaagc ctatatagcg agctgctata
     5341 gaaataatta cacaatacgg tttgttactg gaatcaatcg tgatcaagct tgagtgagcc
     5401 attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggagagtcg ccgtggtttg
     5461 gtaaaaacga cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt
     5521 agtcggattg cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc
     5581 tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac
     5641 ggtgaaatca aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac
     5701 taggttcgcg cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc
     5761 ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc
     5821 tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc
     5881 aacgagtcta tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc
     5941 tggccgccgg gaaataccct ggtcgcgcgc ctgatgaact gctgcggcgg ttatcttaac
     6001 cattacggtg actactcttc cgcgcaaatt gcggaaggtt tgaaccatac ctacggcggc
     6061 tgggcagatg gtaacagccc gtcggatatc gaaaacagta aattggtagt gctgtttggt
     6121 aataacccag gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca
     6181 cgccagaaat ctaatgctcg cataatcatc atcgatccgc gctataccga caccggtgcc
     6241 gggcgcgaag atgagtggat ccctattcgt ccaggaacag atgccgcact ggttaacggt
     6301 ctggcgtacg tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc
     6361 gttggctatg acgagaaaac cctgcccgcc agtgcgccga aaaatggtca ttataaagct
     6421 tatattctgg gtgaagggcc agatggcgtg gctaaaacac cggaataggc ctcgcaaatc
     6481 accggtgttc cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg
     6541 gcgtttatca gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt
     6601 gctatctcga tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc
     6661 gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc
     6721 cagaccagca tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg
     6781 gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg tgccgatcaa aatgatctgg
     6841 aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc
     6901 ctccaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg
     6961 gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgca
     7021 ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg
     7081 cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt
     7141 gagcaacagt ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag
     7201 tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt
     7261 aaaaagcgcg acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag
     7321 gcaaacccac tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac
     7381 attgccgcta cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg
     7441 ccgggctttg aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacaggt
     7501 ttccactata aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcggct
     7561 tgccgtcagg aaatgtggat caacccgctt gatgcccaaa aacgcggtat ccacaacggc
     7621 gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca
     7681 cgaatgatgc cgggcgtggt cgcactgggt gaaggcgcct ggtatgaccc ggatgcaaaa
     7741 cgtgtcgata agggcggttg tatcaacgta ctgaccactc aacgtccgtc tcctctcgct
     7801 aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat
     7861 gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga
     7921 gctggcttgt aaagactaca aagatttgac gccagaagtc agtttccgcc gcatttatga
     7981 atatgctggc ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta
     8041 ctatctgtcg atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag
     8101 cggtgcgatg cataaacgcg aagatggttt tgttgtggtc gatgaagatg tatgcattgg
     8161 ctgccattac tgtcatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg
     8221 ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg
     8281 tgttgaatct tgcccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa
     8341 acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat
     8401 tgtgatcaaa cccaatgcca atagccgccc gacaggggat accactggct atctggcaaa
     8461 cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg
     8521 tcttcgggca atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg
     8581 acctgcgagc agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtactga
     8641 tgggcattgg ctttatcgcc tctatgctcc atcttggttc accgatgcgc gcctttaact
     8701 cgctcaaccg ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt
     8761 ttgctgtagg cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc
     8821 gtacgctatg gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc
     8881 gtgtgtataa cagcattgat accgttccga cctggtacag catctggacg ccgatgggct
     8941 tcttcctgac gatgttcatg ggcggcccgt tactgggtta cctgttgctg agtctggctg
     9001 gcgtcgatgg ctgggcgatg cgtctgctgc cagccatttc tgtactggca ctggtggtga
     9061 gtggcgtgat gtcagtgatg cagggcacag agctggcaac tattcatagt tctgtgcagc
     9121 aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg
     9181 ccgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc
     9241 tactatccgt ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct
     9301 atggtttgca tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt
     9361 cggccccggt tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac
     9421 gataatcagg aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt
     9481 cacggtgcag ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacca gcttgcgaca
     9541 tgcgatccca cgccgaatgc cgggtaattt cattaaaggt ccctgatgca tcagtcacga
     9601 caaagacatc aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg
     9661 ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat
     9721 cttcgttatc ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt
     9781 gtgctttcag ttctagaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa
     9841 tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact
     9901 tatcgggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa
     9961 cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag
    10021 agtcagtggt acgtctgaaa tcggtcagac gcgatgcatt gctctgaaag catagacggg
    10081 aaatatgagt ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg
    10141 ttagatcaat aaaaacaatc agatgagatt aatcatctct tcagcattag cgttatgaat
    10201 gagtaatata aatcgataat accgccgctg cgatatgcgc gtattttcac ccattgacaa
    10261 tgtttttggc ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg
    10321 tccacgtata cccggcctgt catgctttta ctgtctggcc tgcttttgtt gactctggcg
    10381 atcgcggtgt taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg
    10441 caggtaggcg ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca
    10501 gggtatgtca ttaaacgcat tggctttaac cgcagctatt atctgtcctc cttcattttt
    10561 gctgctggct gtgccggcct tggcctgatg attggtttct ggagctggtt ggcgtggcgt
    10621 tttgtcgcgg gcgtcggctg tgccatgatt tgggtggttg ttgagagcgc gctgatgtgc
    10681 agtgggacgt cacgtaaccg tgggcgtttg cttgctgcgt atatgatggt ttattacgtg
    10741 ggaacgtttt taggccagtt actggtcagc aaagtctcaa ccgagctgat gagcgtattg
    10801 ccgtgggtta caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg
    10861 aatcagcagg ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag
    10921 gcgcggcttg gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc
    10981 ctgatgcctc tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg
    11041 gcggtactgg tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag
    11101 tttggtcgac tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg
    11161 atgcttagcc aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg
    11221 ctatatccgg tggcgatggc ctgggcttgt gagaaagttg aacatcatca actggtggcg
    11281 atgaaccaag ccttactgtt gagctatacc gtgggaagtc tgcttggccc gtcatttacc
    11341 gctatgctaa tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg
    11401 tttatctatt tgctgatgct gcttcgcaac gccggtcata cgccgaaacc cgttgctcac
    11461 gtgtaaatga attcaagcaa agtgtgaact tactgtttca cactctgctt ttttgtttct
    11521 tctatctgac ttgctttttt ccaaatttta ttcgtttaaa aataaaatgt gcagcaggtt
    11581 ataattttgc atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta
    11641 cagatgcccc tttaattttg gcgaaggatt tgtctatggc tgggaatgtt caggaagtaa
    11701 gcgccccatc
//
LOCUS       DADLTI010000124        11652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLTI010000124 DADLTI010000000
VERSION     DADLTI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.124"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(36..1400)
                     /gene="tdcG"
                     /locus_tag="JN755_003082"
     CDS             complement(36..1400)
                     /gene="tdcG"
                     /locus_tag="JN755_003082"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7381807.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1472..1861)
                     /locus_tag="JN755_003083"
     CDS             complement(1472..1861)
                     /locus_tag="JN755_003083"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7381808.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1875..3982)
                     /gene="tdcE"
                     /locus_tag="JN755_003084"
                     /pseudo
     CDS             complement(1875..3982)
                     /gene="tdcE"
                     /locus_tag="JN755_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(3992..4168)
                     /locus_tag="JN755_003085"
     CDS             complement(3992..4168)
                     /locus_tag="JN755_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate acetyltransferase"
                     /protein_id="HAY7381809.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTEL"
     gene            complement(4202..5410)
                     /gene="tdcD"
                     /locus_tag="JN755_003086"
     CDS             complement(4202..5410)
                     /gene="tdcD"
                     /locus_tag="JN755_003086"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7381810.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5436..6767)
                     /gene="tdcC"
                     /locus_tag="JN755_003087"
     CDS             complement(5436..6767)
                     /gene="tdcC"
                     /locus_tag="JN755_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7381811.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6789..7778)
                     /gene="tdcB"
                     /locus_tag="JN755_003088"
     CDS             complement(6789..7778)
                     /gene="tdcB"
                     /locus_tag="JN755_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7381812.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7877..8815)
                     /gene="tdcA"
                     /locus_tag="JN755_003089"
     CDS             complement(7877..8815)
                     /gene="tdcA"
                     /locus_tag="JN755_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7381813.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9005..9349
                     /gene="tdcR"
                     /locus_tag="JN755_003090"
     CDS             9005..9349
                     /gene="tdcR"
                     /locus_tag="JN755_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7381814.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9605..10144
                     /locus_tag="JN755_003091"
     CDS             9605..10144
                     /locus_tag="JN755_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381815.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10166..11342
                     /locus_tag="JN755_003092"
                     /pseudo
     CDS             10166..11342
                     /locus_tag="JN755_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         3291 a         2823 c         2531 g         3007 t
ORIGIN      
        1 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
       61 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
      121 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc
      181 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
      241 tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca
      301 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc tgccattgaa
      361 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
      421 ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt
      481 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
      541 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
      601 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctagaaacc
      661 agctgacgat gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct
      721 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct
      781 gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata
      841 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
      901 acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg
      961 ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc
     1021 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     1081 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     1141 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     1201 ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc gtacagatcg
     1261 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     1321 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     1381 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     1441 acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     1501 tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg
     1561 tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca
     1621 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     1681 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     1741 caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa
     1801 cgccctgaac ataagggccg attgcgccta gggcacgttg cgtttcgata atctttttca
     1861 tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt
     1921 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     1981 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     2041 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     2101 aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg
     2161 tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     2221 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     2281 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     2341 gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc
     2401 ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     2461 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     2521 ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg
     2581 ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag
     2641 ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc
     2701 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc
     2761 agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     2821 gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     2881 atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc
     2941 atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     3001 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc
     3061 ggccccatag tgtgcaggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac
     3121 cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg
     3181 aatcaaattc cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga
     3241 tcagttcctg tgcctgctgc tcattgagga cgccagcttt aaagtcgcgc tcaatgtaga
     3301 tatccaggaa cgatgccgtg cgccccagcg acatcgcgcc gccattttgc gatttcactg
     3361 ccgccagata agcgaagtag agccactgca ccgcttcctg tgcattctgc gccgggcgag
     3421 agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct
     3481 ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag
     3541 actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataactgatg ccatacagcg
     3601 ctacgcggcg atagtcaccg ataatgcgcc cacgaccata gccatctggt aaaccggtca
     3661 gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt
     3721 tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat
     3781 aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca
     3841 acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat
     3901 catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt
     3961 tttcgatacg gatgccttcc attacttttt ctcacaattc cgtggtggca ggcgtcgctt
     4021 cggcgagaaa agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga
     4081 cattaatttc gcttttccag tccgtacctt taaagccaag ccatgcgtca gcgtacagct
     4141 tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa
     4201 attatgcaaa ttctgcgggc gcgttaactt tacctaaatg aatggcatcc aaagcaatca
     4261 ttttttcttc gttagtcgga ataacgacgc aaatgacatg cgcattttca ctggaaacaa
     4321 ttcgctcgcc aaaagagtta gatcgattat tcatttctgt atcaatctct acgcctaata
     4381 cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct attccgccgg
     4441 tgaatataat tccatccagc cgacgtaatg aagctgcatg tccggcaata tgacgggcaa
     4501 ttcggtgaac aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt
     4561 tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat
     4621 tcactacgcg ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg
     4681 caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc
     4741 ccattgaggt gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa
     4801 gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcagcgaa tgtgcgcgct
     4861 gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat
     4921 aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg
     4981 tatcaaatac ggccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac
     5041 ttaaatttgc gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga
     5101 cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc
     5161 caattaaggc cacgctgtca tttaaattcc gtttttccag ttcaaatgca attgccttca
     5221 atgcaccttc gtagctgtgg agagccagcg gtgctggctc tcccccattt acggataaga
     5281 atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg
     5341 catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa
     5401 attcattcat ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc
     5461 aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc
     5521 gccagagacg gcgctttacg gatagcatac atcggcaaca ggcacagcag ggatgcgata
     5581 atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc
     5641 caggtggagc ccatgatgaa gatcatgctg agagtattca gtttacccag cgacactttg
     5701 gttttgtcac ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc
     5761 agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat
     5821 tccagtgtaa tcgcgaacgt tgttttcgta ccggccatgg acgcaaagtg gttagccaga
     5881 taagaaagca ctggaatatt ctgcactttg gcttccgcca tgttggccgg agacagagta
     5941 aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa
     6001 atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca
     6061 cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg
     6121 gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg
     6181 aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc
     6241 accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta
     6301 ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc
     6361 agcggtgcaa atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg
     6421 ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg
     6481 ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca
     6541 gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc
     6601 aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc
     6661 ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag
     6721 gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa
     6781 cgaattaatt aagcgtcaac aaaaccggtg atttgagaga cgcgagaaag atcgatattg
     6841 ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt
     6901 aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt
     6961 aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat
     7021 tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg
     7081 gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga
     7141 acgttttctg actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc
     7201 gcaataccag caattaaacc accaccacca attggcacaa tcacgttatc gacatcatag
     7261 agatcttcca taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg
     7321 taaggtggga taaaaatacg accttccatt tcgacaattt cgctcacttt agcgatagtg
     7381 tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact
     7441 ttggattttg gcgcaccttt tggcatcacc actttaccgt cgatacccag tatcgcgcag
     7501 gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt
     7561 tccgcatcgg tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta
     7621 cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg
     7681 gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata
     7741 tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat
     7801 taaatatgta ggtaaccgta accgacacct gcaaaacaga caggtggatt atttatgttt
     7861 aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac ccattataag
     7921 atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt
     7981 tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa
     8041 attgattaga accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat
     8101 tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga
     8161 tgccatttct ttgtaacgta gtaagcagtt cgctgtagta ccccatatta gtttgtggca
     8221 acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact
     8281 tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct
     8341 tcatttctgc acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta
     8401 agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga
     8461 acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga
     8521 cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac
     8581 gggtaatgga ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg
     8641 tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac
     8701 tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa
     8761 tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga
     8821 cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaataa acaggatatg
     8881 tgcgaccact cacaaattaa ctttcattac tttccaaggt atcgttatta caattaaata
     8941 cctttaaata tcaacaagtt aaagcataaa aatcagcata aaccctgatt tctatcaaaa
     9001 aaaaatgacg gggataacca tattttatgg cgataacatc attcgttatg tggttaatac
     9061 aaaaaagggg ctgagaccat atttcaagca attaccggat aattaccagg caaaatttga
     9121 gttaaattta atgagcaagt tctcaaattt tattataaat aaaccatttt cagcgataaa
     9181 tactgcggca cgtcacattt tttcacgcta tttgttggag aacaaacatt tattttatca
     9241 atattttaaa atttcgaata caggtattga tcatctcgaa caattgatta acgtcaactt
     9301 tttctcttct gacaggacgt cattttgtga atgcaatcgt tttccataaa ttcttctccc
     9361 ctcataggcg acgaatagca ttttgtgttg aggatcacaa aacgaataat tgctggtcgc
     9421 cgcgataagg tcagacgaag tcaacaaggg ggattttcac tgtgctttac atcagcgtag
     9481 gacacagaat gttgcttcgt ttactgtaac gccgggtaaa tgagcgtttt ttgatagtgc
     9541 gtaagtgtcg tgcgaggaaa ataagcatct attattgttt gtcatgacga caaggcaggc
     9601 atcaatgaaa ggcttcccaa ttgcacatat ttttcaccct tcaatcccgc caatgcacgc
     9661 agtggttaac aatcacaata gaaatattga ttattggacg gtaaaaagaa agtttgcaga
     9721 aattgtctcc accaatgacg ttaataaaat ttacagtata agtaatgaac tgcggagagt
     9781 attatctgca attactgcat tgaatttcta tcaaggcgat gttccttctg tcatgatccg
     9841 aatccaaccg gaaaatatga gtccattcat catagatatt tctacaggag aacatgatga
     9901 ttatatcata caaacattag atgtaggcac ttttgcacct tttggtgaac aatgtacttg
     9961 ctcagccgtc aataaaaaag agctggaatg tattaaggag acgatttcta agtattgcgc
    10021 aaagttcacc caaaaagaag ccatcttaac accccctagg cactttaata aaacctgcat
    10081 tacttcagat tgttggcaaa ttctcttttt ttcacccgat cattttaata atgattttta
    10141 ttgattattt ataaaggata aaataatgtt ccctgtttca tcaattggta atgatattag
    10201 tagtgattta gtccgtcgaa aaatgaatga ccttcctgaa agccccatag gaaataacct
    10261 cgaagcacta gcacccggta tagaaaaact aaaacagacc tctattcaaa tggtcacttt
    10321 acttaacgcg ttacaaccaa gtggaaaatg cattatcact ggtgattttc aaaaagaatt
    10381 agcgtactta caaaatgtaa ttctttataa tgactcgtct cttcgtctgg attttttagg
    10441 ttataacgcc caaattattc aacgatcgga caatacctgc gaacttacca ttaatgaacc
    10501 gttaaaaaac caggaaatat ctacaggcaa tatcaatgtt aattgcccat taaaagatat
    10561 ttacaatgaa atcaggaggt tgaacgtaat ctttagttgt gggactggag atatagttga
    10621 tctatcctct ctggacttac gtaatgtcga tttagattat tatgatttca cagataaaca
    10681 tatggctaat actattctaa atcattttaa attggattca acaaatttta ctaatgccaa
    10741 catgtttcag gttaattttg ttagttcaac acaaaacgcc acaatctcct gggattattt
    10801 actaaaaata acgcctgttt taataagcat tagcgatatg tattctgaag aaaaaatcaa
    10861 gtttgtcgaa agttgtttaa atgagcttgg agacattacc gaagaacaat taaaaattat
    10921 gagatttgca attataaaat ctataccaag ggcaactctt acagataaat tagaaaatga
    10981 attaacaaaa gaaatatata aaagctcatc gaaaatcaac aattgcttga acagaattaa
    11041 attaccagag atgaaagaat tctcatcaaa aaaatatatg attacatcga tacaatcatt
    11101 gaagattatg aaaatatcaa agaaaatgct tatctggtcg tcccccaaat taattatact
    11161 atggatttaa acatagaaga ctctggctca gaagagttaa taccctcgag aaagacgaaa
    11221 attctccgga caatggcttt gaggtcgggg aatataacac atatgaagca tataactcag
    11281 agaatcaata ttttaccaga gaggactata cgtataatta cgacctttta aatgcaatat
    11341 agacttaata acttcttaca aagtctatta ttaataccgt agatattcat cttaccctgt
    11401 gcaccaaaag caaaaactta tcgacgcggg tttgaaactg atggcaaccg caaaaatgcc
    11461 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
    11521 tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa taaagcgcac tcaacaatct
    11581 gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc
    11641 ttttgggtat ag
//
LOCUS       DADLTI010000125        11553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLTI010000125 DADLTI010000000
VERSION     DADLTI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.125"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JN755_003093"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN755_003093"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JN755_003094"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JN755_003094"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7381816.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JN755_003095"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JN755_003095"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7381817.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JN755_003096"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JN755_003096"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7381818.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JN755_003097"
     CDS             complement(3367..4701)
                     /locus_tag="JN755_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381819.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JN755_003098"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JN755_003098"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY7381820.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JN755_003099"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JN755_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY7381821.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JN755_003100"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JN755_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JN755_003101"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JN755_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY7381822.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JN755_003102"
     CDS             9047..9826
                     /locus_tag="JN755_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY7381823.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JN755_003103"
     CDS             9816..10625
                     /locus_tag="JN755_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY7381824.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILCAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JN755_003104"
     CDS             10712..11146
                     /locus_tag="JN755_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7381825.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11553
                     /locus_tag="JN755_003105"
     CDS             11143..>11553
                     /locus_tag="JN755_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7381826.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         2755 a         3093 c         2951 g         2754 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacaaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaataac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggtgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ctaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cttttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct ttgcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg cat
//
LOCUS       DADLTI010000126        11438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLTI010000126 DADLTI010000000
VERSION     DADLTI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.126"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            9..461
                     /locus_tag="JN755_003106"
     CDS             9..461
                     /locus_tag="JN755_003106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7381827.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JN755_003107"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JN755_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1867..2427
                     /locus_tag="JN755_003108"
                     /pseudo
     CDS             1867..2427
                     /locus_tag="JN755_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JN755_003109"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JN755_003109"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7381828.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JN755_003110"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JN755_003110"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7381829.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JN755_003111"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JN755_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7381830.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /locus_tag="JN755_003112"
     CDS             6055..6456
                     /locus_tag="JN755_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7381831.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JN755_003113"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JN755_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7381832.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JN755_003114"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JN755_003114"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7381833.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JN755_003115"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JN755_003115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7381834.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JN755_003116"
     tRNA            10321..10396
                     /locus_tag="JN755_003116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11438)
                     /locus_tag="JN755_003117"
     CDS             complement(10411..>11438)
                     /locus_tag="JN755_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7381835.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2744 a         2838 c         2946 g         2910 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggttcat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacg
//
LOCUS       DADLTI010000127        11434 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLTI010000127 DADLTI010000000
VERSION     DADLTI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.127"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..655)
                     /locus_tag="JN755_003118"
     CDS             complement(<1..655)
                     /locus_tag="JN755_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7381836.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     "
     gene            complement(673..957)
                     /locus_tag="JN755_003119"
     CDS             complement(673..957)
                     /locus_tag="JN755_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7381837.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(969..1679)
                     /locus_tag="JN755_003120"
     CDS             complement(969..1679)
                     /locus_tag="JN755_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7381838.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            complement(1704..4454)
                     /locus_tag="JN755_003121"
     CDS             complement(1704..4454)
                     /locus_tag="JN755_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7381839.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(4464..4763)
                     /locus_tag="JN755_003122"
     CDS             complement(4464..4763)
                     /locus_tag="JN755_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7381840.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(4774..5436)
                     /locus_tag="JN755_003123"
     CDS             complement(4774..5436)
                     /locus_tag="JN755_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7381841.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(5439..5654)
                     /locus_tag="JN755_003124"
     CDS             complement(5439..5654)
                     /locus_tag="JN755_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381842.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(5647..5955)
                     /locus_tag="JN755_003125"
     CDS             complement(5647..5955)
                     /locus_tag="JN755_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381843.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            6269..7183
                     /locus_tag="JN755_003126"
     CDS             6269..7183
                     /locus_tag="JN755_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7381844.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            7187..8170
                     /locus_tag="JN755_003127"
     CDS             7187..8170
                     /locus_tag="JN755_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7381845.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            8163..8651
                     /locus_tag="JN755_003128"
     CDS             8163..8651
                     /locus_tag="JN755_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7381846.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            8648..10366
                     /locus_tag="JN755_003129"
     CDS             8648..10366
                     /locus_tag="JN755_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7381847.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            10630..10839
                     /locus_tag="JN755_003130"
     CDS             10630..10839
                     /locus_tag="JN755_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381848.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            11048..11182
                     /locus_tag="JN755_003131"
     CDS             11048..11182
                     /locus_tag="JN755_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381849.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(11225..11434)
                     /locus_tag="JN755_003132"
                     /pseudo
     CDS             complement(11225..11434)
                     /locus_tag="JN755_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2986 a         2622 c         2911 g         2915 t
ORIGIN      
        1 ggatgtagtc caggtaatcc attaccaccg tcagggcgat gccgctgaac agggatgtaa
       61 ggatgcagcc cagaataatt ctgagccagt tgctgaacat atcccggaac cacgaccaca
      121 tcaggcagta gataaagatc ggggcggtcg ccagcatcag cagcatcacc agttcggcta
      181 tcaggttaat gatggccgtg atgcctacca gtaacatgac gcctgcccag accagaaact
      241 gcgccagagt gccttttaga gcgacatagg ttgaatcatc ataagagtaa agcttctccc
      301 caagtgcttg agccttttcc cagacggtat ccagccgcgc ccagatcgac gcgtcgccgc
      361 tgaatccgtc ccgcaggccg gtgatggcat ccgaaaccag atcgagatag cctccggcgt
      421 tggtgacgaa aatcatgatg atgagcatcc ggctcacgtc ccacacgatc tcctcgaagg
      481 ggcggactag cttgcctgcc atcgtctggt aggcgcgcca cgtgatgtac agcgagagca
      541 ccgcgatgga gaacaggtgt gccatccacc cccccgtatc ggcggccacg ctggtgagca
      601 cgctgtcaaa cccctccatg atgtggctgt ttaatccgac gaatattcca ctcatggtgc
      661 gttctcctgt cgctaattca ggtcagcaat aggctcttgc acgcgagctt tatcaaggtc
      721 tttcaggatt tgcttatatc cttcggcaac atcaggttta gctttttcac ggtgaagggc
      781 ggagcgtgca ttctggcaat cggtgggcac aaaattatga ttaaaaatgc actccctgta
      841 tttcgccagc atgtcgtcat ggtgtgccat ataccagtct acggaggttt tatcctcgca
      901 gccagtaagc gcggccactg aggccagaac aataaaggtc agtgtcttca gtttcatggg
      961 gtcacctgtt agttaaggtc tgcgattgga gctgtgcgca tttgctcggc ctgcgccttt
     1021 cggcgttgtt cggcaagcag ctggttgcgc atctcacttt gtttgaccga catttcccat
     1081 tgagtggtca gggcgttcag ctggatactc cgcgcggaca ctacgttcgc cagatcctgc
     1141 gattctttac tgtccttcgc attctcgatg cgactggcga gattactaat atcgctcagc
     1201 gtgtcggaaa ttttttcctg gatgtcagaa gtgtcctcga cggacaccgc ctgattgagg
     1261 agcatctgtt tgcagctatc ccggtacgcc tcgctggcgt tctccgggca gttgtcgaac
     1321 agtttgtatt tgtcatacag cctgtttagt tccccagcat aagacccgcc tgagccggtt
     1381 aacagatcgt ttaaggaaat ccccctgttt ttcagatttt taatgtcagc cgtcaggttt
     1441 tttgcctgat tcaggaatgc ctggacgtcc cgaataccgg tggccgttgc cagctgttgt
     1501 ttataagcgt tcatctgcga ctgataatgt tgaaccgtct gctgccactg ctggagcttc
     1561 tccgcccact gctggatgtt gctggcgttg gctacttcat caaaaacggg aataccggcc
     1621 tgagccggga cgtgaaagag acacaaggcg acggtcagcg ccgtcagggt ggttttcagt
     1681 ttcatggtgt tatccttttg tgattaaacg gcacgtctca gcatttcgtc tttccagtct
     1741 tccggcttca taccctcctg ccagatctcg tcaaaaattt tcaggttgtc cgcactggcg
     1801 ctgagcagct tcgtgtagcg acctagcccg gagaggtcga gggtgatttg tgcgatatac
     1861 gggcgcacgt caccacgccg cagcggagat ttcatgatca ccatctggcg ggcgtagggg
     1921 tcgagggtgg tcacgatgtc gtaaaccgct ttcggcaccc gcagcatgtc cacgtagtcg
     1981 tactccttcg cctgctggtt cgccatgaag atgcgggtac tacactgctc gataatggcc
     2041 gccgagatct cgtgcttgca catctcctcc ggcgactgcg tggccgggaa gaaaatgccg
     2101 ttgagctttc gaatgacctt cagcatgtcg agggcgaatt ttgaaaactc cgggtcctga
     2161 agccatttcc agaattcatc catgaaaatg accaggcggc ggccatcgag gaggctggtc
     2221 acgcggtaga gcaggtagaa ggtgagcggg ctgcgcacgt ctttgtcgtt caggaactcc
     2281 gtgccgtcga tgccgaaatt atcaatattg tcgatatcaa aggtgtccac gtcattatca
     2341 aacacccagc caaactcccc gccctgcgcc cactgctgga gacggatgcg cagcccgttc
     2401 atccgtgcgt ccttcgtcgg ggcttcatgc agcacttcca gcatgcgggt gatgccgttg
     2461 cgacgaagct caggcgggta gtccagcatg atggtgtcca ctgcctcgct gaggcgttct
     2521 tcatcgcgcg ggtcgagcgg tttgccgtcc cttgtgcaca tcattcgcat cagcttcttg
     2581 aggaagttgc ggttacgttt cgttggagcg agcgcaaacg ggttaaggcc ggtcttctcg
     2641 cctgaacgga aacggaagta cgagccgccc tggaggcgta tcgacatttc cgccgaacgg
     2701 tctttatcaa agaccaccga ggtgaagcgc tttttcgtcg cccccggcgc aaacgactca
     2761 ggacgccggt atttctgcat cagatgctgg aacatcatca gcagggtcga tttccccgtc
     2821 ccggtactgc cgagcagcat ggtgttaccc ggcggcttct cgctgaactc gtcaacatcg
     2881 gcctggctgt tgtgcagatt cacgtactga catcccccgg acggggtcgg aacaacggca
     2941 atcgcctccc cccaggcatt cccgtcccgc ttgtgcgggt gaaagttgtg aaacgacacc
     3001 atttcggcgt agttgaggct gctcaccggc accagacgcg ggcgcaggtg atacacaccc
     3061 ggcaactgcg cgagatacgc cgccgacagc gactcgcggg acagggtcat gatgaaaccg
     3121 agatccttca gggtatccgc cagggtgttg gtgtcccgga taacctgctc gctggttggg
     3181 gcgctgacaa taagcgtctg gtgatatttc ccgaatgaaa taatgccgga ctgaagcatg
     3241 tcgcgggcga ccttcaggtc ttcctgctgg gagatcgcat catcctcggc agacttcagg
     3301 cgtttctcga tggtgcggat gcagccccgc gcttcatcct tacccatgca ggtataggac
     3361 tgcgtgaaca cgtaccggca gcgggcataa agaagcgagt catacagccc ggtgatggtt
     3421 tcagtgctgt aatccttgat ttccagcccg cgaaagtagc tctggccgtc ggtgtggctg
     3481 acctggccaa tgtcggtgga agggaaaata tccgtgccgc ccagcacgtc ataaaacggc
     3541 gtgcgggtga cgcggacttt ctgccacctg ccggtgatca gaaagttgta aaacgccagc
     3601 tgggaggaga acacgtgccc gttctcctca taagtgccaa gcggccgcgc cgcgtagcgt
     3661 gacagcgccg tgccaatcgc ttgccggtgc tcctgcatca cctgcagggc attgtcgagc
     3721 agggttttac gttcgcccag ggacttctgt ttcatggtcg cccgctcaat gccggtgtgc
     3781 ggattgaagc agacggtgaa gaagaggcga cgctggtaga aaggtttgcc ctcgcgggag
     3841 gcgtaaaaca gacgcgcgat ttcatctgaa aacggaatcc cggattcaca ttcaagcgtg
     3901 tcggtaaatt cgtcgctcac cagatgggta tagaaggtaa cgggtagccc ctcaaaggag
     3961 cggataacgt tgttgagctg catgtccagg gtggcgaggt aatgttcatc ctcgcattca
     4021 aaaatgatac cgccgatctc ccaggtgctg acaaagtcac cttttttgtt cttcaccacc
     4081 tgctcgtgca ggtgggaaga ccaggggaga tagcgggaca gcgggatact ctcgttgagc
     4141 cgcatatgtt ttataaactc cgtaacgtct atggcgtcgt actggtttgc gacaagcgcc
     4201 tttgtcccga aatgccgggt cgccgcgcgg ttgccacgcg ttttaaacgc cagcaacttc
     4261 aggtcaaaga aatgaatgtc ctcttttgcc agccgtctca gctcgataaa ggcaggtatg
     4321 agaagcagga gaagccagta aaaacgtgac cagacggccg ccagggtaag gatgcacagg
     4381 gtgaacagca gggggacaag cgggaccccc cacagcgccg ccggtcgggt cagcgccttg
     4441 ttgaggatgg tcatgctgcc tccttaggcg gcgaagtagg caccgaaccc ggacgccccg
     4501 gcgagcagga cacccgccca gaacacgcgc tggagatcgt gccaggcctt gccgtcaaca
     4561 agcatcttgt aggcgataaa ggccaccgca cagctgatag tgacgactga cataccagtt
     4621 aggccgagga agacccagtt aatggcgtca ctgcctttct gaaatgcctg gtcagcgctg
     4681 gccgccattg ctggaacagc agacagggcg gaaaatgcgg caatcaggcg ggttgcagcg
     4741 gttttgatag tacgtaaagt catgatctct tccttaatca gaagacacag cgaggccgcc
     4801 gcgcacgaca tgtggcggat aagcagcgat gctggtcggg gtggtgtcgg gtgccggaat
     4861 tacgccgcgt atgacgctat ccgggtaaac agtgttgtcg gacgggacgg caggcgcttc
     4921 cccggatgag ggagatgctg tctgctgttc tgcccgcgtt gagggcacaa cgtagccaat
     4981 acgctgtacg tagcttgtgt tgccgaacgc gctttccggg cgctggccgg tttcaaagtt
     5041 gcctgagtaa tagcagctca gggcgcgctg taatgttcct cctcgttgat agcagtcggt
     5101 gatgattttc gcggcaacag tcatgttgtc acaggggtta aacatggatt cggcgcttac
     5161 gccgaactgc gggaagttgg tgctggttat ctgcatcagc cctactgaat aacggtgctt
     5221 tttctgcttt atggcctcca caatttttag cgcctcgtct ttcgacgaag gcagatgaga
     5281 aatcacgcgg ctcctgccgc cctttaccgg gacaatctca gcgatggcat atggatttaa
     5341 gccggattcg gtgcgggtga tgtcgtggat ggtatcgggg tgaacggcgg gagcacactg
     5401 cattgcgagc tgaaacaacg cggcagtggt taacatagtc agtcctttct gtagagcagt
     5461 accttaccgg tggcacagtc cgggttgata aataactgaa tgtcgccctg gctgttagct
     5521 tgggcaacac cgatttttgt aagcgtcatt gccagccgat cacccttcac cgaatacacg
     5581 ttgtgcgtgt actcggtcat gggggtgaat acggcttcaa gcagtgaggc cggagcaggc
     5641 gtgatgtcag gcattacatt tcctattttc cagcagtgag gtgattgcat cgatacgtcg
     5701 cagcctgtcg gcaataagta cggatacatc ggattttttg agtccctgcg ctttcacagt
     5761 cagggtagtg atttctgccc gcagggctgc ccgttcaatt agcaattctc ggacgttatc
     5821 cggcagctcc ctgagcgacc aggtgccatt aatggcacgc gtgatacggt cacgggagga
     5881 gcgtagcggc ggcttgccga gagttgaata aggattctga cgcagcgcct tacggcggac
     5941 ggtttgcatg ctcatggctg tttctcctgg gtatgagtaa gccacccgcg tgtggtgcac
     6001 agtgggagac agggaaggat taaacgggag gcttaacgat acagaggagt gagagtgtta
     6061 cccattacag gcgcggcacg tcatctcttt acgcagtcac agaaaacacc tccttatcct
     6121 gaccggggaa cgccgctgct aggttattaa agagctaatg atttgattaa tacaatagtg
     6181 cttataagtt gggtattcaa taaaattata acttatagta tcgatttaag aatgattcca
     6241 atttttagga ctttttcagc tgtgtaaaat gttggagatg tttgaaaaaa ttgaaaaaaa
     6301 atactggcag gataaaatca agaaaatctc caaattcaga gtaataaaaa tgcaagatct
     6361 cgatgatgca aaagtgatag aggtactggc gaaggtattg accaaaaaag gtatttccaa
     6421 ggtaaagcac aatagttaca taagtaaggt gctacagatc actacatcag cgggctataa
     6481 gaagttgaat ggtgcgacca agtgggaggt tcagcaactc ataaaagtag tccaatccgt
     6541 tggcatggat atgattgagt ttttccgtat ttattatgag gataataaag aaattcaaga
     6601 tgcgatctgg aacgatggcc gcagtgaaca ctcttgtaag gtatgcctct atcctgaagg
     6661 ttcggaagta aacacagaat actcagcact gaaagtagct gaccaatgga acatttttcc
     6721 gacaaatgat ttaaaagatg aactgcttta tgaatcaaaa cgaagcatag aaagtattgt
     6781 cataaatcca aaggtaatta gcagcactaa gtatcgaatt gcactacttg atgacgatgc
     6841 taatatcacc gaaagcttaa aagaaatcct ttcgaacgat tattatcaag tagatactta
     6901 tgataacttg aaaaaactta ctcaagctgt cgcaacaaat ccgtatgacg catatgtttt
     6961 ggactggatc atcggtgatg aaacaagctt taagttggtt aaagctataa ggtcgtcaaa
     7021 aaataaaaat gcaatgataa tcgttcttac aggtcaacta tctggtataa aagatgatga
     7081 aattgtaaag tcaattcatg attatgacat tgtgggacca tacgaaaagc cgattaaatc
     7141 aggcatcatc aaatctaaca ttgaaaaata tttcgccaga tagattatga gaaatttact
     7201 tgaatattcc ggtagcttga taagtaacga tagtttgatc gtagcacctt acgattgtca
     7261 gaagctaagc tatgaaaata ttttaccatt aaacaatggt cttataaaaa actggaatat
     7321 acaaaaattc tcaagtgatt actctgaaat agaacaccta aatgtgatca atggcatggg
     7381 agtgacatta ggtgattcta tcgtcgggat ttctgtgctg tcagcaatca aaaaaaagaa
     7441 tcccaagatt aaaattaagc ttattcgacc ggagactgca ccgtcatatg ttgaggaact
     7501 gtatgaatta gcatcctcgg ttatagatga gcttcattac atgccttttc atattcagaa
     7561 attgccttgt actgcattga atattgatgt gggaaatcag ctatattctc cagatttcca
     7621 tattatggaa atgcatgatt attttatcca aggtcttggc gtgaaacttg atgatgttaa
     7681 ccataatgag ctatctaata aatggttagg caatattgat ctcggagcgc cagtttttga
     7741 aaactacaca ctttttgcac ctattgctag cacaaaaata agaagtatac cgtcgaagtt
     7801 ctattatgac atagttgata tgctttctag gcgcgaagga aaaacggtac taggtttcgt
     7861 ggatgtgaat cataaaaatt atattaacat atcaaagtat tcaccccata cgaaagattt
     7921 catagcgcta attaagtatg ctagcaacgt ttatacttgt gactcctctg cattgcatat
     7981 agccgctgga tttggtgtac ctactgaatg cgtgtttaat acaataccgc cagaattaag
     8041 aacaaagtat tacagtaagt gtaagtcgat ttatgttggg aataaaagat tagaaaaaat
     8101 tcagagtagt gaagatttcc aactggttaa aatggtggaa aataattaca gggagtatct
     8161 ttatggttaa aataatatca ttcttatccc taatgctttt gtcaattagt gcatatagca
     8221 atattgggga tatagttata gatggttcaa tcgctaatgc taacagtgat aaaggttatg
     8281 tgattactga aaagcagttt gattcgttcc cacgggatac catcgtaacg acaacacctt
     8341 ggacgccaac cggtaaaaaa gtaaaattca agggcgtcga cctaagttat gttttaaaac
     8401 tcgctggcgc taatggagca aagctaaaat tccatgctct gaatgattat gaaatcattg
     8461 tagacatgaa tgacgttgaa aaatataaca ttttattggc tacagaaatg gatggaaaaa
     8521 ggcttcaaat aagggatttt ggaccttatt ttttggtata tcctttagat gaacattata
     8581 ttgagcttaa cacgccacac tatcttgcac ggttcatttg gcagatagac aaaattacgg
     8641 tgctcaaatg aaaaaaaatt tctttatcat tttggttgct ctgcttttgt tgatagcatt
     8701 cactgtgtct agtgttgtta agtacaccag tcttttatcg gagaaaaatt tatacaccgt
     8761 cgctggcacc caagaaaact acggtttctc agtggcaaaa ttcattatac agttgaatga
     8821 gctttacacg ctaatcaata gcgataatac tattgacgat gttaggctta aattcgatat
     8881 cctttattct cgtttgaatg ttatttatgt aaagagtgag gcgactgcac ctctatataa
     8941 acagcaaggc tatgaagaaa caattgattc gataaacaaa aagcttgaag acatagacgg
     9001 gctgctttcg cataatcacc ctgattataa aaaaataagt acaatcatag ctgatataaa
     9061 accgctaact aaaaccgtta caaatcttgc tgatatggct gaaatagcac aaaggaatga
     9121 cgctcttaaa gatttcagga gcaaacgtca gcagttatgg actctgcttt ttattacagg
     9181 tgggttgatt tccttacttc tttttgtcct atttatttac ataagtaaaa ttaaccgatt
     9241 gttactatca gaacgggctg cttttgccag taaaaatgca tttttaggta tggtaggcca
     9301 tgaactcaga acatctttac aggcaatagt gtctatcatt gatgtcgtaa ccaataatct
     9361 gagtggaggg attaaatctg gtcagataga acgacttgaa actgctgttt cgaaaatgga
     9421 gcgccagtta aatgatttgg ctgaatttgc aaaaattgac aacggatctg tagagataaa
     9481 aaacacctat aatagcttgc aagcaattgt tactaacgct gtgcaggatt gcattgctat
     9541 ttatgaaaaa aaggatgtca cagttaaaat aaaaaacaat aatgatgcag tgatttttac
     9601 agatgctcta cgccttaatc aagtaattga aaacttaacc agtaacgcta ttaagtacac
     9661 tgagcgcggg gaagttaatg tagattactt tatagaaaaa ggtaaggtat taaatattgt
     9721 catttctgat accggaaagg gaatccctaa agacaaacta aagttcatat ttaaaccatt
     9781 tacacgagtt gtagattcga aatccactgt acctggattt gggatgggac ttgctattgt
     9841 tgcagggata atcagactat taaaaggtac tattcatata tctagtgaag taaatgtagg
     9901 tacaactgtt acagtgagaa tacctatcaa gttgggagat agcagccttg caacagctga
     9961 ggtatctgct tcagctcccg gagatgtctt cggcgttctt agcttgcttg ttattgatga
    10021 taatgaaatg gcctgttcgt ctctttccag tctgcttact aatgctggat acattgtcga
    10081 ggctacaacc tcgcctgaac gagccctgga aaagcttctg cgtaaacctt atgacgttgt
    10141 cctgagtgat ttacaaatgc caggcatgac aggtgatgag ctgtatgcaa ctgttagtag
    10201 cagtcagaat cctaatcgat ccacgccttt catattcatc agcgcataca gtgatgcaca
    10261 ctctatcgga caggctcctt tgttaactaa acctgtcagg ttaaaagata taaaccttaa
    10321 aatacatgag gttatgtcaa atcaaggacg agtcaaaagt gagtaggcat tgtcgtaata
    10381 tttgcgatta tagctttggt gttctcgatc gttgcgaatt ttgaaaattt gtggagtcaa
    10441 atggcgccga aagcccggcg tagccggtta cctggctata aactctgcca ccctaccccc
    10501 tgccctgatt agccttaacc cgcttacagc aatgaagaag taaaagtaat cccttttaac
    10561 cccggtgagc gggctttacg gcgtttacga cgtaaagggc gtgatttccg gggccttagc
    10621 ggcggcagct tgctgctgac gcttaggggg atgttcttca gttatttcag cgacaccagg
    10681 gaacttaaca cggtagttgc aaagctatac ccgctaaaaa tgtctcagat gcctctgtac
    10741 gcggtcggag tggtcccggc aaggcgtcag ccgcagccgg ggcgtatctc cgcgttagcg
    10801 ggccgaaggg ccgcttacga gcgtgtaccc gacaactgac cgcagctcca cccacagcga
    10861 tgaagaagag tagttacaac acccatcatt aaaagcgaca ctactcccgg ccttcggccg
    10921 ggggcggcgg cgcttttcag tggtggggag gggcacccgg ccggcaaacc ggcgccggtt
    10981 ttatgatgag ctttcttttg aatgctgtaa ctaatttggt tctccggtcg gggcccttcg
    11041 cgaagcgatg ggagctcttt ctgatgttgc cgaaccggcg cgaccgtcag gtcgctgcaa
    11101 gagcgggcat tctccggagc ctgtccacag ggtaggcgat gaagaggtta tgtgtttaca
    11161 ggatttatta aaggatcttt aaaagagata cagaactggt gtttaaaagg aactacgcgc
    11221 cctgaaagac cgacggcagg ctcagaagat gctccagcgt ggccatagta cgttcaccga
    11281 agacgtgtgc aacaaccgtc ctccgtatcc tgtcatacgc gtaaaacaac cagcgctggc
    11341 gtgatttagc gccgacgtaa ccccactgtt cgtccatttc cgcgcaaaca atgacgtcac
    11401 tgcccggttg tatgcgtgag tttaccgact gcgg
//
LOCUS       DADLTI010000128        11155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLTI010000128 DADLTI010000000
VERSION     DADLTI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.128"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            34..393
                     /locus_tag="JN755_003133"
     CDS             34..393
                     /locus_tag="JN755_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7381850.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="JN755_003134"
     CDS             complement(513..692)
                     /locus_tag="JN755_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7381851.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="JN755_003135"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="JN755_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7381852.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="JN755_003136"
     CDS             2131..2709
                     /locus_tag="JN755_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7381853.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="JN755_003137"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="JN755_003137"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7381854.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..5941
                     /locus_tag="JN755_003138"
     CDS             4388..5941
                     /locus_tag="JN755_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7381855.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRSDAVNSTVTDIIIALGQ
                     RLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNG
                     HITPIMSLR"
     gene            complement(5945..7495)
                     /gene="yoaE"
                     /locus_tag="JN755_003139"
     CDS             complement(5945..7495)
                     /gene="yoaE"
                     /locus_tag="JN755_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7381856.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            7958..8929
                     /gene="manX"
                     /locus_tag="JN755_003140"
     CDS             7958..8929
                     /gene="manX"
                     /locus_tag="JN755_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7381857.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8992..9792
                     /locus_tag="JN755_003141"
     CDS             8992..9792
                     /locus_tag="JN755_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7381858.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9805..10656
                     /locus_tag="JN755_003142"
     CDS             9805..10656
                     /locus_tag="JN755_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7381859.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10711..>11155
                     /locus_tag="JN755_003143"
     CDS             10711..>11155
                     /locus_tag="JN755_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7381860.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2736 a         2820 c         2817 g         2782 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgttctg acgcggttaa
     5701 ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc
     5761 ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt
     5821 gcaagggtat ttgtacgcac agccgatgcc gctacgtgat tttcccaaat ggctggcggg
     5881 cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgt cgttacgtta
     5941 acgcttactc atcttcatcg tgcgccggtt gctctttaac aatgcgaacc agatcaacac
     6001 gataatcatt ggcttcaagg atggtgatat gcagtggccc tacatcaatc acatcgccca
     6061 cacggggaat gtgaccattt gccgagatca cgaggcccgc gaccgtcgcg atatcatcgt
     6121 catcggcaag gtgctcaaca tcaagcgcct gctgcaaggc atgcaaatct gtaccgcctt
     6181 ttaccagcca gccgttacca tcggtaatga tttccggcgt ttcgtcagcg tccgggaatt
     6241 cacccgcaat ggcttccagc acatccagcg gcgtgaccaa gccttgtacc acaccaaact
     6301 cgttggtcac gataacaaag ctcccgcgag cgcgacgcag cacgcccagc aggttgatcg
     6361 gatcgagggt ttccgggacg ataatcgccg gagacgccga agcaatcgcc gccacatcaa
     6421 cgccctcttc cagcgccacc agcagttctt tagcacgtac gataccgatg atttcatcca
     6481 gttcaccgcg acataccggg aacagactgt gcggtgaaga gagcagttgc tcgcggattt
     6541 catcgacccc gagattagcg tcaacccagc ttatttcacc gcgcggcgtc atgatcccgc
     6601 gcagagaacg cgacgccagc gtcagtacgc cgttaatcat gtaccgttct tcttcggcaa
     6661 atgcaccttc cgggatcggc atcgggttat cggcatcgtg ctgaacattg gcctgacgtt
     6721 tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc aaagtcgact
     6781 ggtgacgaat aaagttgcga cgcgcaatct ggttaaacac ttcgataatg atcgagaagc
     6841 caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct gccaccagac
     6901 tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg tgctggttaa
     6961 cgaatcgcgt cagcggtttc gatgccagca acataaccgc catcgcaatc actaccgccg
     7021 ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca tccaacgaga
     7081 agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg tagcctttac
     7141 cgtggccgga atcatgatcg cagttttcca gccgttcatg cagttcggtt gttgctttga
     7201 acagcaagaa tatccccccg aacaacataa tcagatcgcg tccggagaag gagaaatcca
     7261 tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac agcagcccca
     7321 gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt tgttttggcg
     7381 gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc acaatttcga
     7441 gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat tccatgacaa
     7501 gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa cgacaaaatg
     7561 caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc tgatgctgaa
     7621 atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg ttgttaaacg
     7681 ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc tttgcaaacg
     7741 aatgtgacaa ggatatttta cctttcgaaa tttctgataa tcgaaagtta aattacggat
     7801 cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg caggggtttt
     7861 tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa atacatctgg
     7921 cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta ttgttatagg
     7981 cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt taggcgagca
     8041 ggaaaacgtc ggctggatcg atttcattcc aggtgaaaat gccgaaacgc tgattgaaaa
     8101 atacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc tcgttgatac
     8161 atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag agcattatga
     8221 agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggccc gtgatgatga
     8281 cccgagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag gcgtgaaagc
     8341 actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag caccaaaagc
     8401 ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg gccttgcgcg
     8461 tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag aaaccaatgt
     8521 ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta agacactgct
     8581 cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca aaatgattcg
     8641 cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta ccaacccaac
     8701 agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg tcggtggtat
     8761 ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg aaaaagatat
     8821 cgaggcgttc aaaaaactga atgcacgcgg tattgagctg gaagtccgta aggtttccac
     8881 cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac gtattgtgtt
     8941 gattatcatt cagttttcac acttaagtct tacgtaaaca ggagaagtac aatggagatt
     9001 accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat gggatcaatc
     9061 ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg tatcgttctt
     9121 ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc gctgggctgg
     9181 atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat ttctaccatt
     9241 ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat ccctctggcc
     9301 gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt ccagcacgct
     9361 gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca cgtttcttct
     9421 ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct gtctgttggt
     9481 accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa tggtctgaat
     9541 atcgccgggg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat gatgcgtgct
     9601 ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac caactttaac
     9661 ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca acttagcccg
     9721 aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga tctcgataac
     9781 gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac cgagaaaaaa
     9841 ctcactcaaa gtgatattcg tggcgtattc ctgcgttcta acctcttcca gggttcatgg
     9901 aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc aattcgtcgc
     9961 ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct ggagttcttt
    10021 aacacccagc cgttcgtggc tgcaccgatt ctcggcgtaa ccctggcgct ggaagaacag
    10081 cgtgctaatg gcgcagagat cgatgacggt gctatcaacg gtatcaaagt cggtttgatg
    10141 gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc ggtatttgca
    10201 gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct gttcttcatc
    10261 ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcttatgg ttactccaaa
    10321 ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac ggaaggggcg
    10381 tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca tgtcaacatc
    10441 ccgctggtgg tttcgaccat caccgggcag gatggacaga cccgtgtcac caccgtgcaa
    10501 actattcttg accagttaat gccaggcctt gtaccactgc tgctgacatt tgcttgtatg
    10561 tggctgctgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt cgtcatcggt
    10621 atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact accggggcct
    10681 tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt actgattctt
    10741 ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc ccgccgtaac
    10801 ggccccaccc tgctggcaat tcctttgctc cgccgtggtc gcatcgatag cgttatcttc
    10861 gtcggattga ttgtcattct tatctataac aacgtcacga atcatggcgc gctaataaca
    10921 acatggttat taagtgcact ggctctgatg ggtttttata tattctggat ccgcattccg
    10981 aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga atatagccga
    11041 atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga acagcgtcgg
    11101 ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct tctcg
//
LOCUS       DADLTI010000129        11054 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLTI010000129 DADLTI010000000
VERSION     DADLTI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.129"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="JN755_003144"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="JN755_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7381861.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2927)
                     /locus_tag="JN755_003145"
                     /pseudo
     CDS             complement(2005..2927)
                     /locus_tag="JN755_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3144..4487
                     /locus_tag="JN755_003146"
                     /pseudo
     CDS             3144..4487
                     /locus_tag="JN755_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4712..6367
                     /locus_tag="JN755_003147"
     CDS             4712..6367
                     /locus_tag="JN755_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7381862.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6507..6731
                     /locus_tag="JN755_003148"
     CDS             6507..6731
                     /locus_tag="JN755_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7381863.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6794..7330
                     /gene="rimL"
                     /locus_tag="JN755_003149"
     CDS             6794..7330
                     /gene="rimL"
                     /locus_tag="JN755_003149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7381864.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7325..8305)
                     /locus_tag="JN755_003150"
     CDS             complement(7325..8305)
                     /locus_tag="JN755_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY7381865.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8429..9421
                     /gene="tehA"
                     /locus_tag="JN755_003151"
     CDS             8429..9421
                     /gene="tehA"
                     /locus_tag="JN755_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7381866.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSTWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9418..10011
                     /gene="tehB"
                     /locus_tag="JN755_003152"
     CDS             9418..10011
                     /gene="tehB"
                     /locus_tag="JN755_003152"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7381867.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10314..10982
                     /locus_tag="JN755_003153"
     CDS             10314..10982
                     /locus_tag="JN755_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7381868.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         2854 a         2581 c         2779 g         2840 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggct caagaactct gactgcatgc
     2701 gttaaaaatt gttcgccggg taaggtaagt tgcgccccct gacgaccacg ctcaaacaag
     2761 cgagcgcccg tcagctgctc cagttcattc aatgtattag agagcgcagg ttgactcaaa
     2821 ttaagggttt cagctgcgtg ccccaaagtt ccttgttgtg cgacagctac gaatgtatga
     2881 aggtggcgca aacggatgcg ctgactaaac agactatttt tttccataag cgatgttaaa
     2941 aacgaagcgg tgtcgctgac aagtgaagtt gtttgattat gataacttga ttgtaaaata
     3001 ttattaacaa ttaaagcaat tatgttacag caaaatggat aatattgatg ttttcgcggc
     3061 gagatcacag tttgtaaatt cttcccgcca gagtgaatgc tgttacctac actccagatt
     3121 actgaccact ggaggcagac actatggcga acagcatcac ggcggatgag attcgggaac
     3181 agttttcgca ggcaatgtca gccatgtacc agcaagaagt tccgcaatat ggcacgctgc
     3241 tggaactggt agctgatgtg aatctggctg tgctggaaaa caatcctcaa ctgcacgaaa
     3301 aaatggtaaa tgcagacgag ctggcgcgac tgaatgttga acgtcatggg gcgattcgcg
     3361 ttgggactgc acaagagctt gctactcttc ggcggatgtt tgccattatg gggatgtacc
     3421 cggtgagcta ttacgatctc tcgcaggcag gggtgccggt acattcgaca gcatttcggc
     3481 ccattgatga tgcttctctg gcgcgtaatc ccttccgcgt ttttacatcc ttactccgcc
     3541 ttgagcttat cgagaacgaa attttgcgcc agaaagcggc ggagattctg cgtcagcgcg
     3601 atatcttcac cccacgttgt cgacaactgt tagaggaata tgagcagcag ggcggtttta
     3661 acgaaacaca ggcacaggag tttgtgcagg aagccctgga aacgtttcgc tggcactagt
     3721 tagcaacggt agatgaagaa acctatcgcg ccttgcacaa cgaacatcgg ttgattgctg
     3781 atgtggtctg ttttcctgga tgccatatca accacctgac gccacgtacg ctggatattg
     3841 accgggtgca gtcgatgatg cctgaatgcg gaattgaacc caaaattctc atcgaggggc
     3901 cgccgcgccg cgaggtaccg attttactac gccagaccag ctttaaagca ctggaagaga
     3961 cggtgttgtt tgcggggcag aaacagggca cgcataccgc gcgctttggt gaaattgagc
     4021 agcgtggcgt ggcattaacg ccgaaagggc gacaactgta tgatgatctt ctgcgtaacg
     4081 ctggaaccgg gcaggataat ctcactcacc aaatgcattt acaggaaacc ttccgcactt
     4141 ttcctgacag tgagttttta atgcgtcagc aagggttggc atggttccgg taccgtctga
     4201 cgccttcggg tgaggcgcat cgtcaggcga ttcatcctgg agacgatcca cagcccttaa
     4261 ttgaacgtgg ttgggtagtg gcgcaaccca tcacctatga agatttcttg cccgttagcg
     4321 cggcggggat cttccagtca aatctgggta atgaaacgca gacacgcagt cacggtaatg
     4381 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
     4441 accaagaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
     4501 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
     4561 aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat aaatattgtc
     4621 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
     4681 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
     4741 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcagc
     4801 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
     4861 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
     4921 tgacacgctg gcgacaatga cgctgcaggc ttataacagt attcaatacg acgccgaaaa
     4981 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
     5041 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
     5101 aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata ccaaacaatt
     5161 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
     5221 gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg atgatacata
     5281 tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
     5341 gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
     5401 cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg
     5461 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
     5521 acagctgggc atttcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
     5581 gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
     5641 cggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
     5701 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
     5761 ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
     5821 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
     5881 tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg cattccagta
     5941 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
     6001 gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa
     6061 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcgccaaa
     6121 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
     6181 tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
     6241 tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca gtgaaacatg
     6301 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
     6361 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
     6421 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
     6481 gtgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc
     6541 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
     6601 aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga
     6661 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag gagagcgtca
     6721 aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac agcatggatt
     6781 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
     6841 tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca
     6901 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
     6961 gggtaatgtg atgttgcatc aacgcggcta tgccaaaatg ttcatgattt tcaaagaaga
     7021 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ctgctgaaat
     7081 cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
     7141 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
     7201 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
     7261 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
     7321 cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
     7381 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
     7441 actccacatg gttttcaatt atcaccgcac ctgtaatacg gctctcacct tgtattacaa
     7501 cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc atttccgcca atcaagacat
     7561 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
     7621 gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtagactt
     7681 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg gcaaaatcaa
     7741 aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg
     7801 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg agcaggagtt
     7861 gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt
     7921 gaccaaatat tctacattgc ccgtaaacta atgagtcatg aatggtgaca ctgtcactta
     7981 tatatgcacc ctggctgatt tcactattat cgatccagac gttatccgtt gcgtaaacct
     8041 ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcacc gttccgccga
     8101 acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc
     8161 gatcgatcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
     8221 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
     8281 cttcacttag gcgatatttg cgcatagcgt tttcccacag gtgacttact ataaccgtag
     8341 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
     8401 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
     8461 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
     8521 ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg cgatgatcat
     8581 ctggggatta ttgactagcg catttattac ccgactcata cgctttccgc atagcgtgct
     8641 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
     8701 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgca tgttcagttt
     8761 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
     8821 tcaccctgag gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat
     8881 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
     8941 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gcagttcggg
     9001 agaattaccc acggcactgc ggacatcact cggcattcag ctcgcgcctg cgctggtggc
     9061 ctgtagtacc tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt
     9121 cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
     9181 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
     9241 tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt
     9301 tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca
     9361 gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg
     9421 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
     9481 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
     9541 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
     9601 gccatgagta tcgccaacgt cgagcgcatt aaatccattg aaaatctgga taatttacac
     9661 acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt tattctttcg
     9721 actgtggtgc tgatgttcct tgaggctaaa accatcccag ggttgattgc caatatgcaa
     9781 cgttgcacta aacctggtgg ttacaacctg attgtggcgg cgatggatac cgctgattat
     9841 ccatgtaccg tcggcttcac gtttgccttc aaagagggag aattacgtcg atattacgaa
     9901 ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
     9961 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccggtaag
    10021 cacaaaacgc gtgaaaattc cccacgctga gatgatttac tgttcttctt ttcggtaagc
    10081 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
    10141 acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc
    10201 gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc
    10261 ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta ttcatgcgta
    10321 ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct
    10381 ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag
    10441 aaacaacctc agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa
    10501 gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta
    10561 cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag
    10621 aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc
    10681 gtggggccat taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc
    10741 ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc
    10801 gcctctattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg
    10861 ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata
    10921 ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt
    10981 agttccagac gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc
    11041 cttcagggcg ggga
//
LOCUS       DADLTI010000130        10922 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLTI010000130 DADLTI010000000
VERSION     DADLTI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.130"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JN755_003154"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JN755_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7381869.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JN755_003155"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JN755_003155"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7381870.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JN755_003156"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JN755_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7381871.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JN755_003157"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JN755_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7381872.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JN755_003158"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JN755_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JN755_003159"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JN755_003159"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7381873.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JN755_003160"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JN755_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7381874.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JN755_003161"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JN755_003161"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7381875.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..>10922
                     /gene="glpB"
                     /locus_tag="JN755_003162"
     CDS             9991..>10922
                     /gene="glpB"
                     /locus_tag="JN755_003162"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7381876.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLA"
BASE COUNT         2733 a         2934 c         2657 g         2598 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa ggtatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgagcaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgct
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgct ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgatcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgttgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 ag
//
LOCUS       DADLTI010000131        10879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLTI010000131 DADLTI010000000
VERSION     DADLTI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.131"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            129..1826
                     /locus_tag="JN755_003163"
     CDS             129..1826
                     /locus_tag="JN755_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7381877.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="JN755_003164"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="JN755_003164"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7381878.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="JN755_003165"
     CDS             3116..3400
                     /locus_tag="JN755_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7381879.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="JN755_003166"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="JN755_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7381880.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdxH"
                     /locus_tag="JN755_003167"
     CDS             complement(4274..5158)
                     /gene="fdxH"
                     /locus_tag="JN755_003167"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7381881.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="JN755_003168"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="JN755_003168"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7381882.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="JN755_003169"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="JN755_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7381883.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9590..10690
                     /gene="ompC"
                     /locus_tag="JN755_003170"
     CDS             9590..10690
                     /gene="ompC"
                     /locus_tag="JN755_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7381884.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2674 a         2788 c         2690 g         2727 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacactt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt caccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaacaccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcacctc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc catacctccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcattt cgacttcgat cacatatcga taaataatat tatttcatct atttttctca
     9481 ttgtttttaa tcaccattct attttaaaaa cagtatgtca tttataaatg acgccgttta
     9541 aaattcgttt agaaaatacg tttaacgata attaaaagga ttctgttaaa tgaaattaaa
     9601 aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac gtagcacacg ctgccgaagt
     9661 ctataacaag gatggtaata aactcgacct ttatggcaag gttaccgctc tacgttattt
     9721 tactgatgat aagcgtgacg atggtgataa aacttatgcc cgtctcggct ttaaaggaga
     9781 aacgcaaatc aatgatcaaa tgattggttt tggtcactgg gaatatgatt ttaaaggcta
     9841 taacgatgaa gccaacggct cgcgcggcaa caagacccgt ctggcctatg ctggtttaaa
     9901 aattagtgaa tttggctctc tggactatgg tcgtaactac ggtgtcggct acgacattgg
     9961 ttcatggacc gatatgttgc cagaatttgg tggcgatacc tggagtcaga aagatgtctt
    10021 catgacatac cgtaccaccg gtctagcaac ctatcgcaac tacgatttct ttggcttaat
    10081 tgaagggctg aactttgccg cgcaatatca aggcaaaaat gaacgtactg acaacagtca
    10141 tctttatggt gctgactaca cgcgtgccaa cggtgacggt ttcggtatct cctcaactta
    10201 tgtttatgat ggctttggta tcggtgcggt atataccaaa tccgatcgga caaatgcgca
    10261 ggaaagagcc gctgctaatc ctctcaatgc ctccggtaag aatgcagaac tatgggccac
    10321 aggtataaag tatgatgcca acaacatcta ctttgcagct aattacgctg aaacattaaa
    10381 catgaccacc tatggggatg gttatatctc taacaaagca caaagttttg aagtggtggc
    10441 gcaatatcaa ttcgacttcg gcttgcgccc atcactcgct tacctgaaat cgaaaggcag
    10501 agatctgggc cgctacgccg accaggacat gattgagtat atcgacgttg gtgcgacgta
    10561 tttcttcaac aaaaatatgt cgacctatgt tgattataaa atcaacctga ttgatgaaag
    10621 cgactttacc cgtgccgtag atattcgcac cgataacatc gtcgctacgg gcattaccta
    10681 tcagttctaa cgtcctctcc tgaccagccc ttctctgggc tggtttttga tttaagtgct
    10741 gctgtatgac tccttaagta aaaatgagca atttttgata tttctggtca ttcacctcaa
    10801 ttaatattat tccatatcct ccggggacat cttactataa tatttattga tgagtgatta
    10861 tgaccaatat taatacagc
//
LOCUS       DADLTI010000132        10809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLTI010000132 DADLTI010000000
VERSION     DADLTI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.132"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            87..1172
                     /locus_tag="JN755_003171"
     CDS             87..1172
                     /locus_tag="JN755_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7381885.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1243..1851
                     /locus_tag="JN755_003172"
     CDS             1243..1851
                     /locus_tag="JN755_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7381886.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1949..3340
                     /gene="selA"
                     /locus_tag="JN755_003173"
     CDS             1949..3340
                     /gene="selA"
                     /locus_tag="JN755_003173"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7381887.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQRLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3337..5181
                     /gene="selB"
                     /locus_tag="JN755_003174"
     CDS             3337..5181
                     /gene="selB"
                     /locus_tag="JN755_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7381888.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5371..6522
                     /gene="yiaY"
                     /locus_tag="JN755_003175"
     CDS             5371..6522
                     /gene="yiaY"
                     /locus_tag="JN755_003175"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7381889.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6687..8225
                     /locus_tag="JN755_003176"
                     /pseudo
     CDS             6687..8225
                     /locus_tag="JN755_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(8444..8599)
                     /locus_tag="JN755_003177"
     CDS             complement(8444..8599)
                     /locus_tag="JN755_003177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381890.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8770..9093
                     /locus_tag="JN755_003178"
     CDS             8770..9093
                     /locus_tag="JN755_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7381891.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9099..10235
                     /locus_tag="JN755_003179"
     CDS             9099..10235
                     /locus_tag="JN755_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7381892.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10232..>10809)
                     /locus_tag="JN755_003180"
     CDS             complement(10232..>10809)
                     /locus_tag="JN755_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7381893.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2656 c         2976 g         2600 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgacggc ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact
      181 acaaccaccc ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac
      241 aggtgacggg aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg
      301 aggtgctttt taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg
      361 acctgatgac ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg
      421 ccaacaccac ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc
      481 tgaaaggcag ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc
      541 aaaatttcct cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc
      601 agagtcagct cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc
      661 aacttactga agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct
      721 acgtcactca ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg
      781 tgatggtctt catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc
      841 tgttacgtct gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg
      901 tgttccacgg caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc
      961 agggggtatt gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg
     1021 aactggaccc taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg
     1081 tttactccga ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca
     1141 gttggatgca ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag
     1201 cctgagccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
     1261 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
     1321 gaatttatta atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta
     1381 gggaaagtgc cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc
     1441 gctgaatata ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
     1501 tcgttgcggg tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta
     1561 tcggtgcgtg aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag
     1621 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
     1681 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat
     1741 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
     1801 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
     1861 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
     1921 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
     1981 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
     2041 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     2101 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     2161 gttgacgaaa gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     2221 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     2281 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     2341 tcgggcgctg gcgcagcggc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     2401 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt
     2461 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     2521 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     2581 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     2641 cattcagggg ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct
     2701 ggatgttcca gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     2761 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt
     2821 ctccggcgac aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat
     2881 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     2941 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     3001 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     3061 acaggctccc cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct
     3121 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     3181 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     3241 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     3301 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     3361 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     3421 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     3481 gatggtcgcg tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg
     3541 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     3601 atggcacaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     3661 gtggcgctga ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     3721 gtaaaggagg ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     3781 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     3841 cacgccagcc aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc
     3901 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     3961 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     4021 gaaaccgcca atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag
     4081 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     4141 attgtcgagc ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac
     4201 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc
     4261 ttcgacaccc cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc
     4321 cgcaacacgc tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt
     4381 aaaccagagt atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg
     4441 ttagccgttc acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag
     4501 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
     4561 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     4621 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     4681 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     4741 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     4801 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     4861 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     4921 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     4981 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     5041 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     5101 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca
     5161 ttattatttc cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa
     5221 tttcttatga ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat
     5281 taattttgat aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc
     5341 cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct
     5401 gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga
     5461 tttacccgta ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat
     5521 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     5581 aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat
     5641 agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg
     5701 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5761 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5821 atcatcactg acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg
     5881 ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca
     5941 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     6001 atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     6061 gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc
     6121 ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc
     6181 ggtttctaca acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta
     6241 ttcaacagca aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac
     6301 gtgacaggta aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg
     6361 gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc
     6421 gcggttctgg cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca
     6481 actcacgaag aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg
     6541 ctggcaaatt gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga
     6601 tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt
     6661 atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc
     6721 ccggcgagta tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg
     6781 aatgggtagc ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc
     6841 tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc
     6901 acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta
     6961 agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata
     7021 acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc
     7081 gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa
     7141 ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact
     7201 tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg
     7261 tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg
     7321 atttactgcc gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat
     7381 atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc
     7441 aacaaattat gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta
     7501 agtcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag
     7561 cgctggaagg ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc
     7621 gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg
     7681 aaagcattcg cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc
     7741 acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg
     7801 tgctcacagg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg
     7861 aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc
     7921 cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata
     7981 cgcaatatgg cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg
     8041 ggcgcggcat acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg
     8101 ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg
     8161 agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt
     8221 tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct
     8281 tatcatacct acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat
     8341 aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact
     8401 acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga
     8461 tggtgagcgg gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata
     8521 aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt
     8581 tattcattag tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa
     8641 aaaccagaca gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg
     8701 aaattgcact gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt
     8761 gagggaaata tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg
     8821 atattctatg ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat
     8881 catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt
     8941 atctggccat ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg
     9001 caaatccatg actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag
     9061 ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga
     9121 cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga
     9181 ccatttccac agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga
     9241 actataacca tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc
     9301 cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag
     9361 gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg
     9421 cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg
     9481 cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt
     9541 acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc
     9601 ggcaaaatta tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga
     9661 tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag
     9721 cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg
     9781 gttatgtgac ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc
     9841 cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact
     9901 ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta
     9961 aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt
    10021 cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca
    10081 ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg
    10141 cggtctactc tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga
    10201 ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag
    10261 gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca
    10321 catactggac ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc
    10381 accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca
    10441 cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt
    10501 gcgtgttatc gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg
    10561 cgacgttgaa gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg
    10621 agtgatgttc gagttcgtca ggacggctaa taggatgttt ctcaagatag cgctggctgg
    10681 cataagcggt tacggcatat tgtgtaataa aactggcaac cagcgattca tcttttggtg
    10741 gagcgtagct gattaaaaca tcacaatcat caggaaattc gacgccttca taaaaggcat
    10801 tacgctcaa
//
LOCUS       DADLTI010000133        10461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLTI010000133 DADLTI010000000
VERSION     DADLTI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.133"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN755_003181"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN755_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7381894.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JN755_003182"
     CDS             complement(1151..1237)
                     /locus_tag="JN755_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7381895.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN755_003183"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN755_003183"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7381896.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN755_003184"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN755_003184"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7381897.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN755_003185"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN755_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7381898.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JN755_003186"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JN755_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7381899.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JN755_003187"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JN755_003187"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7381900.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JN755_003188"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JN755_003188"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7381901.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JN755_003189"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JN755_003189"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7381902.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JN755_003190"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JN755_003190"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7381903.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..>10461)
                     /locus_tag="JN755_003191"
     CDS             complement(10298..>10461)
                     /locus_tag="JN755_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7381904.1"
                     /translation="ATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVAL
                     FLTAKVKDV"
BASE COUNT         2737 a         2744 c         2560 g         2420 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctagcgcgta tttttgttcg
       61 cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccatc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aagcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaatgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 acccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccatttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattatt ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca ttaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct g
//
LOCUS       DADLTI010000134        10451 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLTI010000134 DADLTI010000000
VERSION     DADLTI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.134"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            4..435
                     /locus_tag="JN755_003192"
     CDS             4..435
                     /locus_tag="JN755_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381905.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..994)
                     /locus_tag="JN755_003193"
     CDS             complement(467..994)
                     /locus_tag="JN755_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7381906.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(987..1733)
                     /locus_tag="JN755_003194"
     CDS             complement(987..1733)
                     /locus_tag="JN755_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7381907.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1748..3655)
                     /locus_tag="JN755_003195"
     CDS             complement(1748..3655)
                     /locus_tag="JN755_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7381908.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4364..4756)
                     /locus_tag="JN755_003196"
     CDS             complement(4364..4756)
                     /locus_tag="JN755_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7381909.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4753..6954)
                     /locus_tag="JN755_003197"
     CDS             complement(4753..6954)
                     /locus_tag="JN755_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7381910.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(6951..7259)
                     /locus_tag="JN755_003198"
     CDS             complement(6951..7259)
                     /locus_tag="JN755_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381911.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7235..7570)
                     /locus_tag="JN755_003199"
     CDS             complement(7235..7570)
                     /locus_tag="JN755_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381912.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7597..7914)
                     /locus_tag="JN755_003200"
     CDS             complement(7597..7914)
                     /locus_tag="JN755_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381913.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(8005..8406)
                     /locus_tag="JN755_003201"
     CDS             complement(8005..8406)
                     /locus_tag="JN755_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381914.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            complement(8393..9442)
                     /gene="virB11"
                     /locus_tag="JN755_003202"
     CDS             complement(8393..9442)
                     /gene="virB11"
                     /locus_tag="JN755_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7381915.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            complement(9446..>10451)
                     /locus_tag="JN755_003203"
     CDS             complement(9446..>10451)
                     /locus_tag="JN755_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7381916.1"
                     /translation="LVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARERNNLGR
                     DLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSAPKSTSS
                     SNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCAMMQRFV
                     SDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAITELRTPE
                     PGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASGSSPGKD
                     RNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDVYRLRMR
                     "
BASE COUNT         2098 a         2961 c         2952 g         2440 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagatac gtcacggcgg
      721 tgtagcggtc gctgttcgcc ttttcgtcgg ccaccacgcg cacggccacg ccgcgccggt
      781 tagcaccggc gagcgccgtc gcgaccggct tactggtgaa gctgtaggcc gcgacgtcaa
      841 tgctctgcct ggcgttgttt atggcgccga gcacgatttc aagagcaggg cgcccgtcag
      901 acggcgaaaa acccgccgtc aggacgggcg cggcggtggc gaacgcgggc acgcagaggg
      961 cggcggccag caccagaagg gtggttttac gcatagttgt ccagctccag gtttttcagt
     1021 tcatcgaggg tgtaaaagcg gaaaacgtcg cggtgttttg cctccagttg cacatgctgg
     1081 tttttcacga tcacgctggc gatgctgtcg aaaataatct tcagggcgcg ctttttcgcc
     1141 tcgtccgggg cgacgtaaaa cgcgtaccgc cattttttat cgctgatagc cagcaggtgg
     1201 ctcgccatga tggactgata gcgcgccttt gttttgatac gtcgttcggt ttcaacggcg
     1261 atcagcctgc cgtccggcag gctgataagc ccgtcgggac ggtgtttgac gctgtactga
     1321 ctgaggaacg ttgaacggtc gccgttaatc caccctgtcg cgccgttctt ctccagcgta
     1381 aggcgcacta gctggttatc aaggtgatgg tcgagtgacc agccggtcag gcggtagggt
     1441 tcaaagcggg cgggcattat gttgtcgtcc gccgtgagga taacggccag cccgtcggtg
     1501 gtaatgcccc acagggatat tctcgacgcg cgggattcat gcacgtgctt ctgaatcagc
     1561 cccattttct ccaccttctc caacaaccgg tagagagggc ggtcagattt aaaatcaaaa
     1621 agcagcttta tgatttgaaa atcgctgtag gtttcctcct tgagaaaatt cagcagtacg
     1681 cgaatttttt cagtgctgcg tgcataacgt tcctgatacg acgaaataag catagaaact
     1741 ccttatttta taaatccagc aacgaatgta tttcgtcatt tggagcgggc tgtgatgcca
     1801 tcagccactc ctcgtcaggt tgcaccggcg gagtgggtat tggcgttgct gagggttttt
     1861 tggtggttgt tgcgctgacc ggaataatgt caggtgccgg aactgccgga gccgttttat
     1921 caaaatccag atccagcacc ggtttaactt ctgccaccgt agcgactgcg accggcggca
     1981 gggtaaacag ctccagtggg gcttttttaa ccttgagcgg tgagattaac gacgggaacg
     2041 cgagcgcgtt ctggctgaag atatagctga cgaacttcgg caggttctgg agcatattgc
     2101 tgtcgatgta atagctttcc gcctgccgaa gcatgcgctc gttatcgact ttttctgtca
     2161 gcaccgccgt ggtggtgact ttccggcttt catcatcgac taaaatcgtg cccgacatgc
     2221 gcgcaatcca ttcggcggtg tctggatcct gaagtttata taccagctta aacttgcagt
     2281 tttccacaac cgccccaacc acggcgtcgc cgtttaaatc cgccggacag tcgcgcaggt
     2341 cggcaattga ctggtgcgcc attaatatat ggacgccttt atcgcgtgcc gcgcctaacc
     2401 cttcgagcgc cggtttggac aggtgatatt tcacctcgtc cttaaagatg gcgatggggc
     2461 ggatattccc cgcgacgcgg tcgcgcaatt ccgcgagctg gaacaggcga actaaaatca
     2521 tgcgctgcgc ggtaatgatt ttagagttac gcatcgaacc gatgatatag cagcagccgc
     2581 ccttgtcgaa aatctccttc agcgcaaaac cgcctggcgc gttaatgctg ttgagcattg
     2641 ccagctcctc ggttttaccg tgaaaggctt taatcgtctc gccaatgccc tgaacataat
     2701 cgccgttata aatatcccgc acggttgcgc ccggattatc cttcacgaac tgcgccgccg
     2761 ttctcgccgc cttgcggtcg tcgatacggt aaaaatccgc cgcctcgcct ttttccgcca
     2821 gactgaaacc ggccacgaat aattcctcca gatggtcggc ggtgataccg tcgataaggt
     2881 tcatctggta ttcctgttta ttcaggtcga taagcacaaa gggttttccg gcgtccgcgc
     2941 acgctttttt atacaggtgc ggtgcccact cgtcgttttt ggggtcgaga acaaaaacgc
     3001 cctcgtcggc gaggatgctc tgataaagca gaataccggt tacgacgcct ttaccggcac
     3061 cagtggtgcc gataatgtcg gcgtgctggg tctgccattc ttttaacgga atatattgcg
     3121 gctcaccctc gcggctcagc ccgataaaaa tccccttatt cagatcgatg taatccatcg
     3181 gatcgtattc tgtggtgacg ggtaaaattt cttttacccg gcgaacgtcg gttctgcggt
     3241 cgcgctccat gctggttttt ttcaccatgc cgcatttgac cttatcaaat tcaggcgaga
     3301 tcaaacgccg taaaaccacg tgcagaataa aaccggcgac ggtaaacagt agcgccattg
     3361 tccaggtaag cagcggcgga ctggcgatat cttcccggcc ataaatgcgc tgtaaaaacc
     3421 agacggccac tggcgctatc gagccgaaaa caaaacagat catggaaagc agaattaaga
     3481 tcctttgcag aaacgtcggc ttttcctgac cgccgtgttt cgcagaaaat ataaaaggca
     3541 gcatgacgcc ggacagcagc gctaacgcca gttgatgcgc ctgcaaaaag gcgataaagg
     3601 ccaggacggc atttgctatc ggcgacagca aatgattcag tttgtcgaat atcacgatat
     3661 tctccttcac ttaaattcgg tcagcaccat cggtcagtca gaacggtcac aggttgtgat
     3721 cgttctgact gaccgattcc atgctgcaaa agaagtggcc gctaagcggc catgatcgca
     3781 ggaggtacac tcccgcgatc taacggcgtg cggcattacg cgcgggctgg acgcccttac
     3841 tgcatgtcgc atgggggtaa tgtcctggct gaacgcccgg ccaaaacccc caaggtcaaa
     3901 agaggccaca ccgtcgcgcg ctacgcgcga ccacccccta aaagacgcct gttttagccc
     3961 ttttcttcgc cttgttcgtt cctcacaaga cgaattaaag ggaaactagg ctttttaggg
     4021 ggtggtcgcg ccacgctacc ggcttttggc cggagactta ggcacgacgg tgtggggggc
     4081 aagcccccca aataaaccga cgcgtgggcg cgtctttttg ctcgctgggg cggcaaaatt
     4141 tattcggccc cccccggctt ttttcggctc acttggggtg agcgctcaaa aagtggaatc
     4201 ggcgacgcaa attagcaatt tcttcgaaat tgaatttact cgcctcctgc cacttcgcag
     4261 actcaggcgg cctgtcgatt agcaattttt tcaaaattga atcgccatgc tcgccagtct
     4321 tcgaagcccg aaaagccagg tcaaaaccga ccctgtgccg ttgttagtcg gtggtaataa
     4381 ggaaggattc aagaggtttt ccggcgtcaa gttcttgctg aattacaacc ggcacgcgtc
     4441 cgacgcctga ccatttttta tgcacgccgt tttcaaaata ctgatattta gctttgcgtt
     4501 ttgccgactt tttggcggga gttgtactaa ccagctctgt cggtgaaaag ccctccgatt
     4561 caatcagcgc cagcaattca ttgcgctttt cttcccgctg tcggcgtagc ttttcctgct
     4621 cgatatgctc tgcgcgtttt tcatcaagcg ctgctgaaat acgtgcactc agcatctcca
     4681 gaaaggcaaa atcactttca cgtaaaaact ttttcagcgc gcgctctgag gttaattcgt
     4741 cctggatacg gttcataatt tcagctcctt cggcatagat acgttcaatc ctttcagcgg
     4801 atcgcgtggt ggatattcaa aaccgagttc cccggtttgc gcgtttttca gcgtgacgat
     4861 catcgaatag gtgtcgtcgt ttttcttcga tttaaaacct ttaataacgt cggttttgcc
     4921 cttgccgatt aacttctcgg cctgtttttc cgtcagcttt ttgccgcgca cttccagcca
     4981 tattttgaac tgacagcccg tacaggcgca ggttttcggc gtcataacca gctcagaacc
     5041 gcacgacgga catttaaccg cgaggcggtt aagcgccgtt ttaacgggat gtttttccgt
     5101 cgcgctgatg gcaatatcgg cgctctcaac caggcgggaa acttccgcgt aaagaccatc
     5161 aataaagcgc tcgatggtca tttcgccggt ttcaaccgcc gcctgttttt cagcccatac
     5221 ggcggtcata tccggctgcg tcaccgagtc gggcagggca tcaattaacg ccagcccggc
     5281 ggcagttggg ataaatttgc ccttttccac ggtgatgtaa tcgcgggttt tcagcgtctc
     5341 aataatagcc gcgcgggtcg ccggtgtgcc gatgccgccg tgttcatctt ttttgtcttt
     5401 atccttctcc ttgagcaatt ttttaatgcg cggatcggcg acaaaatcgg ccacgcgaac
     5461 gagggcagcg agcagggtcg cctcggtaaa cagcggcagc ggtttagttt ttttctccga
     5521 aatcaccacc tcgcggcagg ttaatgtcgc cccggtttgc aggcggctga tggcctcaaa
     5581 cgaggtgctg ttttcgtcct cctcgtcgtc ttcttccgcg tcgttcagga acgtcgtaaa
     5641 acccgcatcg gtgattttac gcgccgagac tttaaacatc tcgtcaccgc atttaatctc
     5701 cgcgatcgac tcgtcatagc gctttttcgc cacgaactgc gcgagataaa actgcgcgat
     5761 ggtgagataa accgcctttt ccttgtcggt aaggcggctt aaatccggca tgttcgtcgt
     5821 cgggataatg gcggtgtggg cggtgacttt tttactgtca aacgccgcac ttttttgcgc
     5881 cgtgtcggtg gtaaggccgt taaaaacgtc gagcgttttt aaggccgcca ccacctgtgg
     5941 cgcgtcgttg aattgttcat cggacagata agaacagtca gagcggttgt aggtaatgta
     6001 tttactgtcc ttgagcttct gcgtgatatc gagcgtctgc tgcgcggtca gtttgtgcct
     6061 gcggttcata tacacctgga ggcgcagcag gttaaacggc agcggtgcgg ccgtggtttt
     6121 tccccggtcg gtggcggccg cgagcacgct ggccggtttt ccggccagag ccctcgcgag
     6181 tccttccccc caggccttat ccgtcagccg ctttttatcg tccaccggcg cgtattcgga
     6241 cgtctgccag cgggcgcaga caacgtccgt accgctgata aactgccctg cgagctggta
     6301 gtaaaacgcc tcgctgtgcg cctgattgtc gcgccaccgg cgcacaatca ttcccagcac
     6361 cggcgtctgg acgcggccaa ccgaaatcgt gcccttatag cccttatcgc gggctttgag
     6421 ggtgtaacag cggctcatac tgaagccaaa aatcgcgtct cctgcgctgc gcatcagggc
     6481 tttcaggtac atcccacgaa aatcgctgtt atcgcgcagg gagttgatgg cttttttcac
     6541 cgctggcgcg gtgttgtcgt taataagcgc ccgtttcacc ggcgcggtgt ttccggcgaa
     6601 ctccagcacc tcgtcaacca ggagctgccc ctcgtcgtcc gggtcgccgc agtggacaac
     6661 cattttgata tccgggcgcc gaatcagctt caccagggta ttagcctgct ttgccacgct
     6721 cgcttttggc tgaagctgta ccgggtacag cttcagcggc agggtgtcga gcgaccaggt
     6781 ggcgtaggcc gggttgatcg tctccggggg caccatttcc agcacgtggc cgacgcagta
     6841 gctcacgata tcggtcgccg attcggcata gccgtcgtgc cgggtgaagt tgccgcccag
     6901 cgctttcgcg atatcgctcg cgactgcggg tttttctgca atccacagtc tcattcttga
     6961 tctctcctga tttcatccat cgtttcgaag tacatcattc gactttcctg aaagtcgtta
     7021 tcagacagac taaagccatg atccgccgct actgtcatga acgtctcagc atcacggacg
     7081 ctgagactgg tcagaacatc gtgcaattcg cacagggcgc gatattggta gatttcgtaa
     7141 tgcaggccgc agccgcgatg cgcttctttc tcgatgcgag aaatcaggct cgcggcacta
     7201 acggaatcac cacgcacatg cggccacggc ggggctataa atccggtttt tgttgccatt
     7261 ggagtgcatc cttatcgttg agtttttccc attccgccgc ctcgtcgtcg ctcagcggaa
     7321 cggtgtaaaa gaagaccggc atactgagca cggagcggaa gaacgactga cgttctgctg
     7381 gtaatgcctg gaaggtcggc gagtgctgcg cggcgagctg gacgagttcg gacgtgttgg
     7441 cctgaaaatg atcctgtttc tggctgattt ccgacaaaac gagcagcacc gtctgcaaat
     7501 cacctgcagg aagctcctca tctttcagca gcccggaaaa gttattaata gaaacggttt
     7561 gattaatcac gtggtgtctc ctttacggct gccgttttac caaccgttca tacggccaaa
     7621 tttcgagatg atcttgccga caatggcgct gtcagcggac gggcaggcgc tcagtttttt
     7681 ctgccgcgct gcggcggtgt gatcgggtaa aaacgacccc ttttttttct ttttgatgct
     7741 catgaaattt ttgtacatcg ggtcacactc ggcgggactg ctgccgtcga gctttccggc
     7801 caggcacagc accatcgcgc acgggtcatt cgggtcggca ttctggccat cggcggcgaa
     7861 cgccggagaa gtaagggcgc acagcagcgc cgccgtgatt acggtgtgtt tcatgatgat
     7921 ttcctctgaa gggaagcagg gcgctaacgc ccgttggttg ttcaacgtcg gtgacggtct
     7981 gaaggactca gaccgtcacc gacgttatcg gtagaagacg atttctgagg tgtcgcaggt
     8041 caggcagccg gagcgggggc gaaactccac cacacccttg tagccgccct cgcggagcca
     8101 ggttttggcc gcaaagaagc gctggccgtc cggggcattt actaccgcgc gcgaggcgtg
     8161 cgcaaccgcg taccactgtg cggccgggta aatcccgccg ggttcaaaac gcgtaagcac
     8221 ctgcgaccag tgcccttccg tcaccggcga ggccacgata agacggttgg gttcccatac
     8281 cggccgggtg gtcgttaccg gctgcgccgg ttgtgaccag tcaacggccg caggctccgg
     8341 tggcgaagaa cagcccgcca ggaggacgac gatcgccgtc aggccggtca gtttaggcag
     8401 catcattgaa actctccttc atagcctgca tgtgcagggg tttaaaatag acgtcgctca
     8461 tgcggcgcac gttgccgtga acgtccacgc tcacaatgac gtcgacgcag tgcagtacct
     8521 tgcgcagcag cacgtcaacc ggcatgcccc ggcacgccgg atgctcgtag cagcggtcaa
     8581 tcagcccgga gatacactcg cgcgggctgc ccgcgtgcag ggaggacata aacccctcgt
     8641 gcccggactc aatcagcttg agtgcgtccc acgcctcgcg gccacgcacc tcggcgagca
     8701 gggggcggtc ggggttcata cggtaaatcg cgcgcaccag gcgatcagag gtgactattg
     8761 cgccctctcc ggcgtccgcc gggtagaaca gatgaacgta gtttttctgg ctgtaaaact
     8821 tcagctccgg gttgtcctcg atggtgataa ggcgcagcgt ctcgggaatg tagtgcagca
     8881 gcatcttcat gtaggtggtt ttcccagagc ctgtttcccc caccacaaag agcgttttgc
     8941 cgtactccac gcacttttcc ataaaaagcg ggatattccc ggacaggtat atgcggctca
     9001 gctccccgtc ttttgattcc accttttctg taccctgcac ccggtcataa aacccggcat
     9061 cgatatacga ctgatgggtg cgctggaagt agtccggctt acgcagggtg attgagatgg
     9121 tgttacgctc acaggccggg ggcaccacta tctggcagcg ttctcctgta gggagcgtgg
     9181 ccgagaggat aggcgacgtt tcatcaatgg tgtcgttgtt ccacacggcc agcgccctgg
     9241 caaaggacga gcactggcgc agcgtgaccg gcgaggcgtg catttcccac gccccgttga
     9301 tttttgtgtg cagctctccg gggcggttga tggcaatttc cgtcagcccc tcccggctca
     9361 ggtagtcccc gaacaacaga tcgcgcaggt gtgacagcgc gacgttgccc tctgacgggg
     9421 tatcagtcag tttatcgtcc atcgcttacc tcatgcgcag tctgtagacg tctgaaaaat
     9481 caatgtccgc ccccgtgacc aggccaatca cgtcaccctg gttgaggtac atcgtcggcg
     9541 ggatgttgat gctgttttcc aggagcgttt ttgccatttc cgccgtggcc gcgcgggtgt
     9601 tctccgtgta gtccgtgttg cgatccttgc ccggcgacga gccggaggcc gcagcggcaa
     9661 agtcctgtac cgtgccgagc aggagggtat tacctatccg ctccagccag tgattgtcta
     9721 tccagcccgc gacgccgttc tcgccaagcg cccccacggc ctggctgtca accatcggaa
     9781 taaccaggcg gccgggttcc ggcgtgcgca gctcggtgat ggcgaggaac agacgcccct
     9841 gcccgttttt cagcgtgccg gtgcggtaca ggccgcgcgc ctcagtgcct gccgggataa
     9901 ggcgcacggc gttgctggcg ctgtaaatgt cctcgctgat aaggcagtta aagcgcccgc
     9961 ccacgtccga tacaaagcgc tgcatcatcg cgcaggggat atgccggtcg gccggaatgt
    10021 acaaatccgg atcgaggccg agccgcttca cgccggtaat gcgcgccagc ccgttatcct
    10081 gcgactgccc ggcgggtgtt gcagagcctg gcgcggacgc gctgcccgca ggtgatacgt
    10141 tactgctgga cgtgcttttc ggggcgctgc ttgcagtggt tggggcgctg tctgccaggg
    10201 cgctgacctt gtcaaacgtc agggggggcg gtgatgcctg cggcggcgcg gcggtatcag
    10261 tcggagtagt cttctcctgg ccggttgtgt ctgccagctg tcccagcggg ttcagatccc
    10321 gtccgaggtt attgcgctcg cgggcggggg aggtggcgtg cgattccggt tcatggctcg
    10381 cgctttttcc gctgccgtca cccttcagtt tcgccatcag gtaatccccg ccggtggcga
    10441 taatgaccag t
//
LOCUS       DADLTI010000135        10437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLTI010000135 DADLTI010000000
VERSION     DADLTI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.135"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(33..3041)
                     /locus_tag="JN755_003204"
     CDS             complement(33..3041)
                     /locus_tag="JN755_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7381917.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
     gene            3205..3762
                     /locus_tag="JN755_003205"
     CDS             3205..3762
                     /locus_tag="JN755_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7381918.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            3945..4805
                     /locus_tag="JN755_003206"
     CDS             3945..4805
                     /locus_tag="JN755_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7381919.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            5015..5554
                     /locus_tag="JN755_003207"
     CDS             5015..5554
                     /locus_tag="JN755_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7381920.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(5526..6362)
                     /locus_tag="JN755_003208"
     CDS             complement(5526..6362)
                     /locus_tag="JN755_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7381921.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6362..7165)
                     /locus_tag="JN755_003209"
                     /note="aph(3'')-Ib"
     CDS             complement(6362..7165)
                     /locus_tag="JN755_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7381922.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(7226..8041)
                     /gene="sul2"
                     /locus_tag="JN755_003210"
     CDS             complement(7226..8041)
                     /gene="sul2"
                     /locus_tag="JN755_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7381923.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            8065..8142
                     /locus_tag="JN755_003211"
                     /pseudo
     CDS             8065..8142
                     /locus_tag="JN755_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8371..8547
                     /locus_tag="JN755_003212"
     CDS             8371..8547
                     /locus_tag="JN755_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7381924.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(8729..9733)
                     /locus_tag="JN755_003213"
     CDS             complement(8729..9733)
                     /locus_tag="JN755_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7381925.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(9812..>10437)
                     /locus_tag="JN755_003214"
     CDS             complement(9812..>10437)
                     /locus_tag="JN755_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7381926.1"
                     /translation="TKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQ
                     GTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGE
                     ARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERAAHALRGNG
                     HAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         2465 a         2794 c         2758 g         2420 t
ORIGIN      
        1 gggtttgagg gccaatggaa cgaaaacgta cgctaagaag ttaattcatt gtttaaattg
       61 aaatttagtt ctctcagttc ccccttcaaa atatcctccg gtagcgtaaa cgtataatgc
      121 cccagcatat tgatatgccc gtgcatcagt ggggacaacc gggcgatatc ttcaaccccg
      181 atttcttctc cattactgcg tatccagctc agggcttcct gcatataaag tgtgttccac
      241 agtaccactg cgttagtgac caggcccagt gcccccagct gatcttcctg accttcacga
      301 tagcgctttc tgatctcacc gcgctgcccg tagcagatcg ccctcgccac agcatggcgg
      361 ccttcccccc ggtttagctg cgtcaggatc cgccgacgat aatcctcatc atcaatataa
      421 ttaagaaggt acagcgtctt gttgacgcgc cccacttcca tgatcgcctg tgccagccct
      481 gatgggcgcg agcttttcag caaagagcga atgagttctg aagcatgaat ggtgcccagt
      541 ttcagcgaac cggcggttcg catcatctca tcccactgat cctcggcttt cgacagatcg
      601 gcacaaccac gtgccagttc gtccagtgca ccgtaatttg ccgatttatc cactcgccag
      661 aataccgctt caccggcatc ggcaagccgg ggggaaaact ggtatcccag cagccagaag
      721 aggccaaaaa taatgtcgct ggtaccggct gtgtctgtca tgatctcaac cggattcagc
      781 cctgtctgct gctccagaag gccttccagc acaaaaatgg aatctcgtaa tgtgccgggg
      841 acaacgatgc cgtggaatcc agagtactga tcagagacga agttgtacca ggtgatgcca
      901 cgtccggaac caaaatattt tctgttaggt cctgaattga cggttttcac cggcgtgaca
      961 aagcgcatgc catcagctga agccacttcg ccaccacccc agcggccagc aagctccagt
     1021 gtggactgaa aatcaaccag gcgggcattg gcgctgacca gcgtttctgc ccggaggtaa
     1081 ttctgtttta cccagctaag ccggtggcgc gtcagcgccg gtatattgtg ctttatcagc
     1141 ggttccagcc cgatattgca ggcctctgcc atcaggaccg cgcacaggct gatgtgcaga
     1201 tcctgtgccc gggccccgga ttcactgaca tgcgtaaact cacgtgtaaa tcccgttctg
     1261 gcatctattt caagcaacag ttccgtcaaa tctactggcg ggagtagcag ccttacccga
     1321 ctgttgagac gatgcaacga tgttggctcc tccagtttct ccaggctgct gatagtcagg
     1381 gaaggatgtt taccgtcatg gcaaatatga acctccgcat ttccttcaaa gcgggatgcg
     1441 acggttttcc aggtttcatc tagctggacc gccaactgtt gcacgccttt atgtccatca
     1501 gtgggatgtc ccagcgcccg gcaaacgggg acccgctgag cctgccattc ttccccctgc
     1561 aacagcttct cgcggggatt tccccagcga tcgctgtttt cgagccagat gtcccttcga
     1621 cgtagtgcat cctgaaggcg ctccagcaga caaagcgagt aaccggcacg ctgtatccgg
     1681 ccctccgcat cgtataccag gcgtttccag gggccggtaa taatatgttc aggcgcattg
     1741 tccaggatgc gctttttcga gccgttcagt tcggtcagat aatgaatggc ggacaacgta
     1801 tgttctccgg ctggcgctgc ctggaaatgc aggtcgcgca ataccgccgg aagaaagcgc
     1861 tttacccgcc cgtactgctc caccatttcg tcatggaaat tgttgttctg aggccgggcc
     1921 agctcattta ccttgctgac ggattctgcc agcctgcttt tcggtatgct gttgaatatg
     1981 gtctccctca gttcagcatc gtcagcctgt tcatccagca acaatgaaca tgcccgcgcc
     2041 aatattagcg cagcacgatc aagatcttta agcgtcctga gccgtttttt ctgccccgtt
     2101 ttcttcgccg cacgagtgat atccagtatc agcatatcga gcacatcaac ggcctcatcc
     2161 agcgccgcag tctcctgcgc tttaacgaat gctgtgagta cggccagttt tctctgctgt
     2221 ggcattcgcg cgatatattt taccgacgcc atgcctgcgt aacgggccaa attacgcagt
     2281 tgtatagcag gcagaccggt aaaattcagt cgggaaaatt ccaggtttcg cagccggata
     2341 taccgctcca gcgcttcggt aaatgccggg ccgctgacgg tgaccggtcc ctttttcagt
     2401 tgctccagcg gtgaagtacg ctgcccctca ggaatgtcca gaagctccgt cacgcgggcg
     2461 gtctgccagc tgtccggcaa cgcggccagt ttcttccaca gccgctgatt tgcccgttcg
     2521 cgtatttcac tgacgagacg tacaagtgtg gttgctccgg gcagcaggac cttattctga
     2581 agcagccacg cggtggcaaa atcaaacata aggccgggtc gctcattact gagccatgcg
     2641 cgggtgtaca gcaggcgctt caggcggaaa gaccagggga aatcaccaaa ttcatggtaa
     2701 ccgtaatatt ccttaatcag cgcagtatgc tctctgaggg tggtaacccg ttctgcgtag
     2761 cgggtaagga cttccggacg gcgaatatta agctgcaccg cgacaaattg ctgaacacct
     2821 ggcagaaccc gggttaagtc tgtcagaaag gtgcccagaa aacgggcggt ggtgagctga
     2881 agcgcaattc ccagccggtt atgccttccc cgccgctggt taatgaaggc aagatcccgc
     2941 tcatcaagat gaaaatagcg cgccagctgc acgtcattgg gttctgcaac atagcgacca
     3001 taattcagtt cctgctcagt ggtcagaaaa tcgacgggca taccggcctc cctgcctgat
     3061 ggtcatgaat taacaatttc gcaaccgtcc gaaatattat aaattatcgg acatcgtaaa
     3121 actgttacat taatatgtct attaaatcgt aaatttgtaa taatagacat gagttgtccg
     3181 atattcgatt taaggtacat ttttatgcga ctttttggtt acgctcgggt ctcaaccagt
     3241 cagcagtctc ttgatcttca ggtcagagca ctcaaagacg caggtgtgaa agcaaaccgt
     3301 atatttaccg ataaggcatc cggcagttca acagaccggg aagggctgga tttgctgagg
     3361 atgaaggtgg aggaaggtga tgtcattctg gttaagaagc tcgaccgtct tggccgcgac
     3421 actgccgata tgatccaact gataaaggaa tttgacgctc agggcgtggc agtccggttc
     3481 attgatgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg
     3541 gctgtggcac aggctgaacg ccggaggatc ctagaacgca cgaatgaggg ccgacaggaa
     3601 gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg
     3661 acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc
     3721 tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3781 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3901 aataaccctg gtaaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3961 ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4141 tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc
     4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4321 ccatgagtga taacactgct gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4921 gtgagttttc gttccactga gcgtcagacc cctaaaagaa ctttccgcta agcgatagac
     4981 tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc
     5041 cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta
     5101 tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga
     5161 attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca
     5221 tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagctg
     5281 taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa gtgccgcctt
     5341 gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga gcttcccgtt
     5401 tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     5461 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     5521 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     5581 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     5641 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     5701 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     5761 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     5821 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     5881 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     5941 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     6001 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     6061 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     6121 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     6181 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     6241 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     6301 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     6361 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     6421 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     6481 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     6541 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     6601 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     6661 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     6721 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     6781 ttcgcgacag cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa
     6841 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     6901 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     6961 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     7021 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     7081 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     7141 caaaaaaaat attagttcga ttcaatggag gttccttcag ttttctgatg aagcgcgaat
     7201 atagagaaat atcccgaatg tgcagttaac gaattcttgc ggtttctttc agcgccgcca
     7261 ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac
     7321 ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc gacatccccc ggaccacggc
     7381 ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag aagcaccggc aaatcgaagc
     7441 gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga ggtttcggga gcagccccca
     7501 gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg tttgataccg gcacccgtca
     7561 gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg ccagcgggtg
     7621 cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg
     7681 atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg
     7741 ccacaccacg cgacaaggca taggcttgcg tcgcgggttg ataactgtcg agcgagacgg
     7801 gaatgccatc tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg
     7861 acgaaacagg cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc acatctgccc
     7921 cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc
     7981 catcggagaa actgtccgag gttatgttga cgatgccgaa aatgatgagc gatttattca
     8041 tgggggcttc tataataata ataatcgagc atgagtctca tacggatgct cgggtcgaaa
     8101 gggaatcccc aggcgagtaa cctgtttgcg gtgctccatt agctgcagga gcagaatagc
     8161 atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag
     8221 gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca
     8281 attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt
     8341 gggtatcgga gacgaagaag gacaaggtac atgctgaccg ataccaagct gcgcaatctc
     8401 aagcccaggg acaaactcta caaagtgaat gaccgggaag gtctctatgt gggggtcgcc
     8461 tcagaaaacg gaatctatgg tcactcccgt ttttgcaaca ccgattttga cgacaagttg
     8521 gcttgcttga atctatccgg cgtctgaatg ggattttatt cccgcgcctc gatgagttcc
     8581 gcgcctgatg aacctccaga aaatatacgg cttcaatgag cctttccgtt ttacaggttc
     8641 ctcaacaggc cggtgggccg ttagtatcat caatatcagt attcgcaaaa ccagatgaat
     8701 gattgtttaa actggtgtat ttctgccttt atgcttcgta agtttgctgt cgcgccgtca
     8761 gtgcccaggc tattctggcc agcttgtttg ccagagcaca ggtgacgaca aagttgcttt
     8821 tccgacacaa caactccctg acccagtcgg ccaacttgcc agactggtgt tccagttttt
     8881 gtatgaatac cctggcacac tgaaccaaca aagttcggat ctttttgttg ccccgcttgc
     8941 taatccctaa caatgtcgtc cgacctcccg tgctgtactg tcggggtacc agccctgttg
     9001 ccgccgcaaa gtcacggctg ctggcgtact gcttcccgtc gccaatctca gttgaaatag
     9061 tactggcagt cagcgttcca acgcagggaa tactcagcaa gcgctgtcca acctcatctt
     9121 cgtccaactt tcgtttcaac tgagattcca gatctttaat ctgctcaaca agatagtgat
     9181 aatgctgttg taatttcagc agtaactggc tgagataaag aggcaaacta ctgtcctcaa
     9241 gaagggtact cagtcgacta ataacggcag cacctcgcgg aacgctgata ccaaattcca
     9301 gcagaaaagc atgcatctga ttagttgttt tcaccttatc ctgaaccagg gattcacgga
     9361 cacgatgcag agctcgcatt gcctgctgag attcggttct gggctgcacg aaacgcatag
     9421 atggacgtga tgctgcttca cagatagctt cagcatcaac gaagtcattt ttgttgcttt
     9481 taacgaatgg gcggacaaat tgcggtgata tcagctttgg aaaatgccct aactcttcca
     9541 gcttgcgtgc cataaagtga gaaccgccac aggcttccat cgcgatggtt gttgccgggc
     9601 atgtcgccag aaattcgatt agctttggtc gggtgaattt tttacggtaa acggccttcc
     9661 cacgatgatc ctgacaatga atatggaaag agttcttacc cagatcgata ccaataagcg
     9721 caatgttttc catgatggtt ctccgaatga aagcctgtcc tcagcatagt actgggaagg
     9781 agggagtgac catctcatta aataaagcac gctaagccgg ttgcagcggt cgtagcggcc
     9841 tgaacttgcc cgcgccgatc ttggcgctgc tgcgccagag gtaatcgccg gtgaggttga
     9901 tgtgctccca accgagcggc gacaggtact gcaacagcgc gtcatcaacg gcatggccgt
     9961 tgccacgcag cgcgtgcgca gcccgttcca gatagaccgt gttccacaac acgacggcag
    10021 ccgttaccag attgaggccg ctagcccggt agcgctgctg ctcgaaactg cggtcgcgga
    10081 tttcacccag gcggttgaaa aacactgccc tggccagcgc attgcgcgcc tcgcccttgt
    10141 tcaggccggc atgcacgcgg cggcgcagtt ccacgctttg cagccagtcc aggatgaaca
    10201 gcgtgcgctc gatgcggccc agctcgcgga gcgccacggc caggccgttc tggcgtgggt
    10261 agctgccgag ctttcggagc atcagggagg ccgtcaccgt gccctgcttg atcgaggtgg
    10321 ccagccgcag gatttcgtcc caatgggcgc ggacgtgctt gatgttgagc gtgccgccga
    10381 tcatgggttt cagcgcgtca taggcggcgt cgcccttcgg gatgtagagc ttggtgt
//
LOCUS       DADLTI010000136        10429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLTI010000136 DADLTI010000000
VERSION     DADLTI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.136"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            38..817
                     /gene="srlD"
                     /locus_tag="JN755_003215"
     CDS             38..817
                     /gene="srlD"
                     /locus_tag="JN755_003215"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7381927.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            922..1281
                     /gene="gutM"
                     /locus_tag="JN755_003216"
     CDS             922..1281
                     /gene="gutM"
                     /locus_tag="JN755_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY7381928.1"
                     /translation="MVSALITVAVIAWCAQLALGGWKISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            1348..2121
                     /gene="srlR"
                     /locus_tag="JN755_003217"
                     /gene_synonym="gutR"
     CDS             1348..2121
                     /gene="srlR"
                     /locus_tag="JN755_003217"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7381929.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            2114..3079
                     /gene="gutQ"
                     /locus_tag="JN755_003218"
     CDS             2114..3079
                     /gene="gutQ"
                     /locus_tag="JN755_003218"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7381930.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(3076..4590)
                     /gene="norR"
                     /locus_tag="JN755_003219"
     CDS             complement(3076..4590)
                     /gene="norR"
                     /locus_tag="JN755_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7381931.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            4777..6216
                     /gene="norV"
                     /locus_tag="JN755_003220"
     CDS             4777..6216
                     /gene="norV"
                     /locus_tag="JN755_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7381932.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            6213..7346
                     /gene="norW"
                     /locus_tag="JN755_003221"
     CDS             6213..7346
                     /gene="norW"
                     /locus_tag="JN755_003221"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7381933.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(7474..9726)
                     /gene="hypF"
                     /locus_tag="JN755_003222"
     CDS             complement(7474..9726)
                     /gene="hypF"
                     /locus_tag="JN755_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7381934.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(9879..10406)
                     /gene="hydN"
                     /locus_tag="JN755_003223"
     CDS             complement(9879..10406)
                     /gene="hydN"
                     /locus_tag="JN755_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7381935.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         2505 a         2911 c         2753 g         2260 t
ORIGIN      
        1 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
       61 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
      121 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
      181 cgaatatggt gaaggtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
      241 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag
      301 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
      361 gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
      421 ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag
      481 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
      541 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct
      601 gaaatcgccg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
      661 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
      721 agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
      781 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccgttaagg
      841 atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
      901 ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg
      961 tgtgcgcaac tggccttagg cggctggaaa atttctcgtt ttaaccgtgc cttcgacaca
     1021 ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcactttaa accgcgggtc
     1081 gtggtcgcca tcgcgctgga cgatcagcag tgcatcgtcg acaccttgtt tatgaaagga
     1141 ctgactgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggcatgca tgcgggtgat
     1201 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
     1261 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
     1321 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg
     1381 gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc
     1441 acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
     1501 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaacg
     1561 ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat
     1621 ggcgattcga tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg
     1681 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa
     1741 ctggataacg aacaaactat cctgatgcca ggcggaacat ttcgcaaaaa atcggcctca
     1801 tttcacgggc aactggcaga gaatgccttc gagcatttca ccttcgataa attgtttatg
     1861 ggcaccgacg gcatcgatct caatgcgggc gtaaccacgt tcaacgaggt ttataccgtc
     1921 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
     1981 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct aattaccgac
     2041 gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
     2101 accggagaga gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga
     2161 gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
     2221 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
     2281 tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc
     2341 ggcagaagca ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat
     2401 ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc
     2461 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc
     2521 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
     2581 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
     2641 ttttaatgaa gaagattttg cccgctccca cccagctggg gcactgggcg ctcgcttgct
     2701 gaataaagtg catcatctga tgcgtcgtga tgacgctatc ccacaggtag cgttaaccgc
     2761 cagcgtgatg gatgcgatgc tggaactcag cagcaccggt ctggggctgg tggcggtatg
     2821 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
     2881 aggcggcggc gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt
     2941 acaggcacaa agtcgcgcca tcgacgccaa agaagttctg atgaaacgca aaatcactgc
     3001 cgcaccagtg gtagatgaaa acggcaaact caccggcgca attaacctgc aggatttcta
     3061 tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc
     3121 gacgtcagtt tccagcattc gcgcacaggc tgcccagttg tgatgatttt gtgccagtgc
     3181 ctggcgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
     3241 gggcaccgcc gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc
     3301 ctcaagaatc acttcatcgc cgttgcgggt ggctctcgcc agcactaccg cccgatgaat
     3361 agcatgttcc agttcgcgca cgtttcccgg aaaactgtag tgttgcagta aatttcgcgc
     3421 cccggcactt aataccacgc gggagagccc ctgccgcaaa cgacactgct cgcagaaata
     3481 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
     3541 cacgctcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa
     3601 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt cattgccaac
     3661 gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
     3721 ctcgccgatc tcatcgagaa acagagtacc gttatccgcc atttcaaact tcccgctgcg
     3781 gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac
     3841 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
     3901 ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac
     3961 gttgagatcg gacgccgcca caatctcaat ctcttttttc agttgcgtca tgccagggga
     4021 caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcagcat
     4081 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
     4141 ggcaatcagc cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
     4201 gagcgtcagt gcgccgatca ggttttgtcc gacaaacaat ggcagaccaa cgcaggcgtg
     4261 aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc
     4321 tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg gatgcccttc
     4381 cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa
     4441 ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt
     4501 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
     4561 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
     4621 attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt
     4681 tatttataga gtaaaaacaa tcagataaga aactggcacg caatctgcaa ttagcaagac
     4741 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
     4801 attcattggg tgggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa
     4861 acgctgcgcg gcagcagcta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
     4921 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
     4981 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg
     5041 accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac
     5101 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
     5161 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
     5221 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
     5281 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc
     5341 gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg
     5401 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc
     5461 gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat
     5521 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
     5581 gctgacgcta tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc
     5641 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
     5701 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
     5761 gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg ctttcggatc tcacggctgg
     5821 agcggcggtg cggtggatcg cctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
     5881 ctcagcctga aagccaaatg gcgaccagac caggacgctc tggagttatg ccgtgaacac
     5941 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
     6001 gtagttaaag aagaaacctc tgccacgacg acggctgacc tcggcccacg aatgcagtgc
     6061 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
     6121 ggaacgccgt ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa
     6181 gacgtctttg acgaactggc atcggaggca aaatgagcaa cggcattgtg atcatcggtt
     6241 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat
     6301 taaccctgat tgccgccgac agcatagatg agtacaacaa acccgacctc agccatgtta
     6361 tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgggc
     6421 agtttaatct gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccatg
     6481 tggtgaaaag ccagaataat cagtggcaat acgacaagtt agtgctggca accggtgcca
     6541 gcgcctttgt cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
     6601 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggcggtg
     6661 gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa
     6721 tcgacaacgc tgccagtatt ctggcgtcat taatgccacc agaagtaagc agccgcttgc
     6781 agcatcggtt gacggagatg ggcgttcatc tgctgttgaa atctcagttg caggggctgg
     6841 aaaaaacgga ttctggcatt ctggcaacgc tggaccacca gcgtagtatc gaagtggatg
     6901 cggtaattgc tgccaccgga ctgcgcccgg aaacagccct ggcacgacgc gccgggctga
     6961 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
     7021 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctgcag ccgattcaac
     7081 ttagcgggat ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactaccgg
     7141 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
     7201 gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg
     7261 acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
     7321 tattgttgaa aacgttgccg gtgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
     7381 ctggcgcgtc ttatccgacc tacggggacg catgtgtagg ccggataagg catttacgcc
     7441 gcatccggca agggtgtcca aatgcaacac gctttatccg ttctggactt cacccgctaa
     7501 ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa
     7561 actctgtgga aagagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa
     7621 acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca tcgtggcctg
     7681 ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg
     7741 attaaccggt gcctgccagt tcagccactg ctgccagaaa gtggcgagat ccagttgatt
     7801 gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg cgagcgcctc
     7861 cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc ccagtgccgc
     7921 cgccactgca tcgaacaacc gcccacacga tgatgccagc ggcgcgttaa ttccacgctc
     7981 aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt cagggtaatt
     8041 ctgccactcc ggcacaaagc gcaggcactg cgccagcagg tttcgccacg gctgcttcgc
     8101 cgccaaatcg ccacccggaa gcgccactgc aggcaagccg cccaggtgct cacattcgcg
     8161 atagttcacc cgcaggcact cgccgcccca caaagcgcca ttctccccca taccgatacc
     8221 gtcgagcgtc aaagcaatga catcaccgcc atccagcggc cactgatgct ctgccagaca
     8281 cgccgctgca tgggcatgat gatgcagtac cgtttgcgtc ggcagattca tttcgctcgc
     8341 ccactggctg gagacatagc ccggatgcac atcatgcaca atgtattgcg gagtgaaatc
     8401 gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc atctggatgc catcgtcact
     8461 taaatcgccc agatgctgac tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt
     8521 tttcagatcc gcgccaagac acagcacagg cggaacattt ttaaagcccg gaggcaaaac
     8581 cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac
     8641 tgaatcatcc atccgctgca cgatgtcgcg gttatgtagc aagaatccgt cggcaatgcc
     8701 ctgcaaatcc gccagcgcct gttcgttgct gatagctggt ggtttaccgc tcaggttgcc
     8761 ggaggtcatc accagcgggc attgcagttc ctgtaacagc aaatgctgga gcgggttcgc
     8821 aggcaacatc accccgactt cgttaaggcc aggggcgata tcatcacaaa gctcaggtac
     8881 gtatttttta tccaccagca caatcggcgc agcgggcgtg gtaagcaact ggcgcgcagc
     8941 gtctggtaaa ctttctgcca ctggcaacat gactgccagc ggtttcgccg ggcgatgttt
     9001 gcgctcccgc agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc
     9061 gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag ctgcctgtaa
     9121 tgccgcctct tgttctgcat gttcaccatg acttacccat tcaagatgcg ggccacactc
     9181 cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt actctttgtc
     9241 acaggccgga catagcggaa acgccgccat cacggtaaac gggcggtcgt aaggcatcgc
     9301 gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg ataaacggat aacgataacg
     9361 ccgctcgcct ggggtattca tttcggcaag gcaagcaggg caagtggcgg catccgggac
     9421 aatttgcgta ttcatggtgc cgccggtgct ctggcggatg gtgaactcgg tgggcagttg
     9481 tgaccagata aacggctcac gctcgacgct atcaatacgc gccagtggcg gacagtgctg
     9541 atgcaattga acaagaaacg tttccggatc ttccagcagc cggacttcta cgccatcgcc
     9601 gtcattacat acatcgccgt gaagatttaa ttgctgtgcc agctgccaga caaacggacg
     9661 aaaaccgacg ccctgcactt tgccacgaat acgcagttgg acaccgcaag atgtgttttt
     9721 tgccattgag ttattcccgc catcatgaat tgcgtaaccc gccctgccgg acacgacagc
     9781 gtcgtatccg gcagtctcag gtcggcgata ccgcccgctc cgtattctac gaatatttcc
     9841 gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag cgccgtacgg
     9901 cggcgttttt ctgcgctcag ttgttcaagt ttattacgat cgacacaaat cagcgcatgg
     9961 gtcgggcaag ccgccataca cgccgggccg tcttcacgat ggttgcacag gtcgcattta
    10021 ttggcttcgg ctttgtcagc ccgtacattc agacccgcgc cgctgttgcg aatcacagga
    10081 cgtaccacca cttccatcgc gccatacggg caagccacca cgcaggtttt gcaaccaatg
    10141 caacgttcct gcatcacatg aacaaaccct ttatcacagc tgatagcacc attcgggcag
    10201 acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca
    10261 cctttaatga catggatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga
    10321 ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc
    10381 gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtt
//
LOCUS       DADLTI010000137        10413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLTI010000137 DADLTI010000000
VERSION     DADLTI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.137"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..182
                     /locus_tag="JN755_003224"
     CDS             <1..182
                     /locus_tag="JN755_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7381936.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            525..1241
                     /locus_tag="JN755_003225"
     CDS             525..1241
                     /locus_tag="JN755_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7381937.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1697..1772)
                     /locus_tag="JN755_003226"
     tRNA            complement(1697..1772)
                     /locus_tag="JN755_003226"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Asn,seq:gtt)
     gene            complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JN755_003227"
                     /pseudo
     CDS             complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JN755_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3505..3580
                     /locus_tag="JN755_003228"
     tRNA            3505..3580
                     /locus_tag="JN755_003228"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Asn,seq:gtt)
     gene            complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JN755_003229"
     CDS             complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JN755_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7381938.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JN755_003230"
     CDS             complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JN755_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7381939.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5914..5989
                     /locus_tag="JN755_003231"
     tRNA            5914..5989
                     /locus_tag="JN755_003231"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5947..5949,aa:Asn,seq:gtt)
     gene            complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JN755_003232"
     CDS             complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JN755_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7381940.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JN755_003233"
     CDS             complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JN755_003233"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7381941.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JN755_003234"
                     /pseudo
     CDS             complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JN755_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JN755_003235"
     CDS             complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JN755_003235"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7381942.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(10034..10297)
                     /locus_tag="JN755_003236"
                     /pseudo
     CDS             complement(10034..10297)
                     /locus_tag="JN755_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2961 a         2631 c         2212 g         2609 t
ORIGIN      
        1 gtgtttcaga taaaccgtta catggtgaga ttaagcttcc cggccaggct aaccgttttt
       61 atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg
      121 cagaaggcga ccgactgcat tcacgtaaat tacgaacctt taatgagccg ccgttccgat
      181 aataagttat gaaccttctt atcagaaata gatgtgaagg attaagtaag acatctggca
      241 aatagcctgc aataaacgcg ggctgcttgc ttccttgggc agatgcgagt tttattatcg
      301 tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc
      361 ccgccaacaa cattagtttt tataaaatta aatataagat tatggcttta gaatattttt
      421 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
      481 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      541 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      601 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      661 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      721 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtacaggga cgttatgaag
      781 gctttggtcc taatggctca atgattattg ctgagacatt gacttcaaat gttaaccgta
      841 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      901 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      961 atatttttga aattttgctt gacgctgaag ttgatgttcg tgatgtaact gaagaagaag
     1021 gtaatattgt tatttatact gaacctgctg accttcataa aggaatcgcg gcgctaaaag
     1081 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
     1141 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
     1201 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatttt aaagaaatct
     1261 gtctttatgg cagatttctt taatctcata taattcttat aaaaaatata atgtttaact
     1321 cgtcatattg cttataaaaa atccccgttc ccagagaaat aatatttatt aaaatggcag
     1381 gtcttctttt tctgattacc gaagacaaag tggcaattac gtatagtttc ccgataaaga
     1441 cgcgacagct acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg
     1501 aacagaattt ttgaccttgt gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
     1561 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg ccactttttt
     1621 acaaagtggc cgggtaaaaa taatttttat tttttaactg tcctgagtct catagagatg
     1681 tctcaaaact aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
     1741 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
     1801 tgcccaccca caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct
     1861 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gctcggatcg
     1921 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
     1981 agcccagtag gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgattcc
     2041 ctgcacataa ccgactacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
     2101 gtaacgggca tcacgagcac ccttaaatcc agcgggtagc acccatgagg cggaccaaat
     2161 aggcataaat aaagcattta gctaaatcag aatcacaacg acgtgtttaa cctgtggatc
     2221 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
     2281 taatccaaga gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     2341 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg cacttccggg
     2401 taagttgata agagccgcaa ttgagaacgc gataaaattt ccggcaataa cgctggtccc
     2461 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     2521 actcgctgga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     2581 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     2641 tgcaactgcg ccaatataac gagaaatggt taaacccaac ccagccccga ccaggagaaa
     2701 aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc
     2761 agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg
     2821 gcagcggctg gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact
     2881 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc
     2941 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
     3001 gctcgtcgtc gatcccgctt accgagacta aatgctacaa cgacagtagt accaagatcg
     3061 atagctgcaa aaaaagccat aatgaccata ttgaagctgt ccgccaaccc cacgccggcc
     3121 atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggccccccat caacaggaca
     3181 cagacgttct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
     3241 ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag
     3301 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga
     3361 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
     3421 gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc
     3481 gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt
     3541 aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt
     3601 ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc
     3661 tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccaaaca
     3721 tagttacgct gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga
     3781 tgccgcgtat ccaggcggat taaattctct tcctcttgtt cgccgctgga ttgttcggca
     3841 actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca
     3901 cttaatacaa tatctgccgg caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc
     3961 cccgtaattc cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat
     4021 ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacaaaac
     4081 cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca
     4141 gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc
     4201 cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc
     4261 tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca
     4321 agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg
     4381 cccggttcag tcatccccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt
     4441 tcatcctcca gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc
     4501 gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg
     4561 aaattcacgg taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg
     4621 gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca
     4681 attgccactg ccttttttcc atcactggag aactaatcac tgacattaac aactctttca
     4741 ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac
     4801 tcattgaagg agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg
     4861 cagccgattc aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg
     4921 caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat
     4981 caacacgaag tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag
     5041 cattaacatc aacgttttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt
     5101 tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc accgccatat
     5161 cgagttggtg atttatcagt ttttcgttaa gcactgcgcc actattttca tgaagataga
     5221 taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg
     5281 cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat
     5341 gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa
     5401 tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta
     5461 actcaccttc cagtatggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt
     5521 cagcagcctg ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga
     5581 agttcatgtt gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag
     5641 tatgcaagat gcctgccagt tctattttgg cgttgatgcg caagctaacc aactgtcaaa
     5701 taagagatta tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc
     5761 tataaaagga gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac
     5821 tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg
     5881 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     5941 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     6001 gcctgctttc gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt
     6061 gaaccagaac taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga
     6121 tttacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     6181 acttttcggt cagactcata ttctgcacga ttgcgtgaca gcggctcatg aacttccagc
     6241 cactttgaac catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacgt
     6301 gtgcctacag gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag
     6361 ccctgactta cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     6421 ctgccaatat aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg
     6481 ggcaaactct cccctcgttt cgcatattcg cgccgggtgt tcggcgttgg cgtccaggtt
     6541 ggtgcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     6601 tggccgatac caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga
     6661 cgcatttcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     6721 tgcggaatgg tgagttgtga gccaggcttc ggtaaaaaaa catcagcgcc cggattcgct
     6781 tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta
     6841 ttgtgatcag gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt
     6901 aatggataag ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga
     6961 atatttacac gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag
     7021 cttttatcag acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac
     7081 aatattactg gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat
     7141 ggacatcgcc agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg
     7201 ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg
     7261 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     7321 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc
     7381 tattccgacc aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt
     7441 tggttgattc aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt
     7501 cggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     7561 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac
     7621 accgtttttc gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     7681 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     7741 gataagcccg gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc
     7801 gcccgcttgt gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg
     7861 tatggctgtc acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc
     7921 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc
     7981 tgccagttgt atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgagtaaccc
     8041 gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag
     8101 taaatcggca attatttgca tgtcatgtct ctttatagca gtgccagcag gaagaccagt
     8161 tcaccaagct ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg
     8221 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     8281 atacccagca ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt
     8341 cgcgccaatg aacacattgc caagcccttc ttcccgcgca taacgatgac gatacatcaa
     8401 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     8461 gccacgcaga gccaactcgc ttaacaccag aatctttgcc agtaccacaa aaatcaatgc
     8521 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     8581 acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga attccccggt
     8641 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg
     8701 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt
     8761 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     8821 cagacgcgta ataaatgaga gcatcgccca aaataatttg ctcatttgat ttttactcca
     8881 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc
     8941 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     9001 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     9061 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     9121 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagcc
     9181 tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     9241 cgccagtgct ccgggcggct ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     9301 aggatttgcg aggtagcgat atacagaacc tgtgaagagt ccccaataag cacctctgca
     9361 tggcgactct tcccgctcca tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat
     9421 tcaaatgtat gatggtatac attgaacaga gcaatggatc ccccaccgtt gctgaccgca
     9481 aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca
     9541 ttacgaaata agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa
     9601 tgcgtcattg acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc
     9661 tactgccgga ccaaccagca aacgggcatt gtgttctgaa atccaggcta ttgattcaac
     9721 gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca acgcaataga attatccgga
     9781 atggtggcga ctatgcacta gaacaccaca atttcgctct ctcggtaagg cactgtcaca
     9841 gcattcaaca gaatgtgact tgccagattt attagcgcca ccagatgttt aaccgggtaa
     9901 ccacgaccca gtcggggata tattccatct tctcgctgac atcatcaaga ataaaaaggt
     9961 ttatcacact cagagcaacg ttcttcagca ggcgggataa ttcttcaaca tttaaaagat
    10021 gtgttattaa aggcatctca aattggacag ggctgccggt tttctgttgc aacaccgttg
    10081 ctctgcttga gacagaactg ccatatgcag catctgatac tttgagtttg accaaatcac
    10141 agccccgtag cttactgtcc aaagccatgt tgaacagagc tagatcgcgt gttttacctt
    10201 ccaactcaag tctaatccgg atcccccaga tatgagatat ctgaagtggc cttttttgac
    10261 cgatggtacg gtctttattc cacggtgacg ttttcatatt caaatctcct gcaatgtggg
    10321 agatttgagt atggttgtct ctcaagagcg aaaataagtc aactatcact agttgttgcg
    10381 catgtacgta ttctgtcaat taattattga caa
//
LOCUS       DADLTI010000138        10379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLTI010000138 DADLTI010000000
VERSION     DADLTI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.138"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            14..1280
                     /locus_tag="JN755_003237"
                     /pseudo
     CDS             14..1280
                     /locus_tag="JN755_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            1332..2487
                     /locus_tag="JN755_003238"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="JN755_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="JN755_003239"
     CDS             complement(2453..2575)
                     /locus_tag="JN755_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381943.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..3839
                     /locus_tag="JN755_003240"
     CDS             2778..3839
                     /locus_tag="JN755_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7381944.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            3933..4463
                     /locus_tag="JN755_003241"
                     /pseudo
     CDS             3933..4463
                     /locus_tag="JN755_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4501..6873
                     /locus_tag="JN755_003242"
     CDS             4501..6873
                     /locus_tag="JN755_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7381945.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6918..9713
                     /locus_tag="JN755_003243"
     CDS             6918..9713
                     /locus_tag="JN755_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7381946.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            10075..>10379
                     /locus_tag="JN755_003244"
     CDS             10075..>10379
                     /locus_tag="JN755_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7381947.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3068 a         2290 c         2241 g         2780 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
    10261 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
    10321 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaa
//
LOCUS       DADLTI010000139        10293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLTI010000139 DADLTI010000000
VERSION     DADLTI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.139"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..401
                     /locus_tag="JN755_003245"
                     /pseudo
     CDS             1..401
                     /locus_tag="JN755_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JN755_003246"
     CDS             484..741
                     /locus_tag="JN755_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381948.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JN755_003247"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JN755_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY7381949.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JN755_003248"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JN755_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY7381950.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JN755_003249"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JN755_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY7381951.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JN755_003250"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JN755_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY7381952.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JN755_003251"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JN755_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY7381953.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JN755_003252"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JN755_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY7381954.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /locus_tag="JN755_003253"
     CDS             complement(8323..8844)
                     /locus_tag="JN755_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY7381955.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9120..9324
                     /locus_tag="JN755_003254"
                     /pseudo
     CDS             9120..9324
                     /locus_tag="JN755_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..9422
                     /locus_tag="JN755_003255"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JN755_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JN755_003256"
     CDS             9762..10019
                     /locus_tag="JN755_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY7381956.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2400 a         2734 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agc
//
LOCUS       DADLTI010000140        10256 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLTI010000140 DADLTI010000000
VERSION     DADLTI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.140"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="JN755_003257"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="JN755_003257"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7381957.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="JN755_003258"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="JN755_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7381958.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="JN755_003259"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="JN755_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7381959.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3268..4124
                     /locus_tag="JN755_003260"
                     /pseudo
     CDS             3268..4124
                     /locus_tag="JN755_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="JN755_003261"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="JN755_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="JN755_003262"
     CDS             5927..6439
                     /locus_tag="JN755_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7381960.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="JN755_003263"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="JN755_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7381961.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="JN755_003264"
     CDS             9032..9235
                     /locus_tag="JN755_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7381962.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="JN755_003265"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="JN755_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7381963.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2445 a         2670 c         2650 g         2491 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtgga
//
LOCUS       DADLTI010000141        10166 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLTI010000141 DADLTI010000000
VERSION     DADLTI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.141"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..367)
                     /locus_tag="JN755_003266"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="JN755_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(511..1134)
                     /locus_tag="JN755_003267"
     CDS             complement(511..1134)
                     /locus_tag="JN755_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7381964.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(1131..2819)
                     /locus_tag="JN755_003268"
     CDS             complement(1131..2819)
                     /locus_tag="JN755_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7381965.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(2968..5595)
                     /gene="gyrA"
                     /locus_tag="JN755_003269"
     CDS             complement(2968..5595)
                     /gene="gyrA"
                     /locus_tag="JN755_003269"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7381966.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5742..6464
                     /locus_tag="JN755_003270"
     CDS             5742..6464
                     /locus_tag="JN755_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY7381967.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(6592..10166)
                     /gene="yfaL"
                     /locus_tag="JN755_003271"
                     /pseudo
     CDS             complement(6592..10166)
                     /gene="yfaL"
                     /locus_tag="JN755_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2360 a         2826 c         2480 g         2500 t
ORIGIN      
        1 actgttccac caagggatat tttttcagcg gggaaaactg gttgttctgt acataacggg
       61 aaggtttaat aatggcattg ccgagatgta attcgggtaa tgcctgagtc acattctgcc
      121 gtgactgaga agagaaagta cgctgcatca cgcggcgaga tttgccgtac cagttatccc
      181 acagctaggt cagcgtattg ttcagcccgt cgcccagata ttgcggttgc accacaatgc
      241 gatgcaggtt tttctgctgg cgcaaaaatg ccatcgggtc aggaatacga tacaggcgaa
      301 cgtccacgcc gccgtactct tccatctgat aacgccgata atcacgcccc ggcgcttcca
      361 gtcgcacttt cgcctcttca ctgctgctaa aactgctgtc agcaagcaaa aagaatgttc
      421 ccccggcggg cggcgcatag ttactggaag gaagcgaatc atcagcttta gcaagccctg
      481 ttcccacaag agacagggac aagcacgctg ctatcgcgcc agaaaattta aacgataaat
      541 gccaatgaaa ttgggattgg aatcgttggg tatccatcgg gtgtccttcc atgtcataag
      601 ttgttgcaga ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc cggtgtggtt
      661 gatgacgtaa cgccccatcc agaccattaa gtgctgggca tcgccctgat cgaaaaaaat
      721 catatcgcca ggcagcgcct ggtttatgtc ttggccaata aactggctgt tgtactgaat
      781 caaattaatc gcggtcacgt aggggccgtt tttcccgctc ccctgattcc agttttgcgc
      841 cagttgacgc tgttcaggtg ttagcgtcat ctctggcggc aaatactggc tagataaacc
      901 gttacttttt agccatttac tgtcgtgaac tttcagcgtc tcgttcgccg caaatcgcac
      961 caggcccgca caatcctgct gataccagcg tggacttggc ccctggcgga gctgttcttg
     1021 tgcaatgcgt acaaaccagg cgcgaaacag cctggattgt tcgacattca gcatttcgct
     1081 atgggcaaca acacaacaca gccagcaaat cagcgccagc agcccgtgtc tcatagcggc
     1141 tgccaggtta tgggtagcca ctgccaggcg acaccgggtt ccatctgggc cagcttcatg
     1201 acataacgcg gttgttgaga taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg
     1261 ttataaaaaa ccggttcgag attcttcggc agactggtca acgtttcgtt acgcagcagt
     1321 ttcgccacgc cttgtggatt gatatagagc ggaacgatgc catcagtggg tattacatcc
     1381 accattgccg ggcgggtttt attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg
     1441 agggagaaaa gcagcgtttt gttttgcatc gccagcgaca cacggaaaaa ataatccgac
     1501 attaattgat cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg ggaactcact
     1561 tcgcgacacc agatttgcgc ttcgccctgc tgagtctggc ttaccggcaa aacaccttct
     1621 ggcgctttgc tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg caattgcgcc
     1681 tgttcggcag tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt tgagtcttca
     1741 taccagcaca gccccacggc accgtctaac gccccattca gcttgtcgtt ttcctggctg
     1801 atgtgcgaaa gcatctcttc ggcaataccg tgtgaatacg gcaccgccac acagaagctg
     1861 gctccggcag gcatactgtt ccagaccggc gtaaaatcga aactggcatc tacgctggcg
     1921 gattcatcat ttaacgccac aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg
     1981 ccagcaaaag aaggcattaa ccgctggtag ccaaaaccca gcaacctggc gctggccacg
     2041 atgcgcaggc gtactggcgt tttttcggct gtacgctctt ccaggccaaa gctggcttgc
     2101 cagcgttttt taccgctcaa caaatcactt gcgatcgccg tggcttcggt atcctgctga
     2161 tcatctttaa acaacatatc cgtactggaa aacaccagca tcttgtcctg atatgtcgcg
     2221 aacatcaggg cgttattgcc gttatagcgc aactgataaa caggaatagt ttcactattt
     2281 atcttgatgc tactgatttc cgttttgctt aactggctgt cgctggtagc ggcaaacaac
     2341 aatggctcca gcagtttgct taaaccactg cgctggatca gcaccatgta atgtgaaaga
     2401 tggccttgtt tatcgtgcca tagcgccgcc tgcgcgggct gatctaagag tgacgaaaag
     2461 agcttatctt tcagcgtgag atcgtgttca tagacaatgc ggcgaatgct gccttcaatg
     2521 cccagacgat ctgcatgatt ctgataatag aaaacgaaat cctcgctcag aacatcgtgg
     2581 agaaacggaa tggtgaggag atctttggga agctggctca gagagtcgct gtctagaaag
     2641 aggtccggct cattgagatc gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt
     2701 ttttctggcc cactgccagc atattgcaac gcccagacgc cagcggaaag cagtgctatt
     2761 gcgccaaaac ctacaagacc atagaaccgc cagcctttcg cctttttttc accactcatt
     2821 gccacattcc ttgtgtatag ccagccattt tttacgggca cagccaaact ttaccgcgcc
     2881 ctaatacgac aaaagcccag actttgcagc ctggactttt caattcaaac aagggagata
     2941 gctccctttt ggcatgaaga agtaaaatta ttcttcttct ggctcgtcgt caacgtccac
     3001 ttccggagca atttcatcat ccccttccgc ggcactgccg tcgatggtat ccagatcttc
     3061 ctcgtcaacc ggttcagcaa cacgctgcag acccactacg ttttcatctt ccgcagtacg
     3121 gatgaggatc acgccctggg tgttacggcc cacgatgctg atttctgaaa cgcgagtacg
     3181 taccagcgta ccggcatcgg tgatcatcat gatctggtcg cagtcatcta cctgcaccgc
     3241 gccaaccact aaaccgttac gttcggtaac cttgatggag ataacccctt tcgtcgcacg
     3301 cgacttggtt gggtattccg ccactgcagt acgtttaccg taaccgtttt gcgttgcggt
     3361 gaggattgcg ccatcgccac gaggcacgat cagagagacg actttatcgc cttcacctaa
     3421 gcgaataccg cgcacaccag tggtattgca gcccatcgca cggacagaag actctttaaa
     3481 gcgcaccact ttaccttcgg cggagaacag cattacttca tcttcgccac tggtcagatc
     3541 aacgccgatc agctcatcgc cgtcaaccag tttgatcgcc actttaccgg cggtacgcag
     3601 acggttgaat tcggtgagga cggttttctt cacagtaccg ttagcggtcg ccatgaagac
     3661 tttcacgcct tcttcaaact cggtcaccgg caggatcgcg gtgatacgtt cgtcctgttc
     3721 cagcggtagc aggttgacga tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg
     3781 gtaaactttc atcgaataga cgcgaccacg gctggagaag cacagaatat ggtcgtgcgt
     3841 gttcgccacc agcagtcggt cgataaagtc ttcttcttta atacgtgcgg cagatttacc
     3901 tttcccgcca cgacgctgcg cttcgtattc agaaagcggc tgatatttaa cgtagccctg
     3961 gtgagagagc gtcacgacca catcttcctg ggtgatcaga tcttccaggt tgatgtctgc
     4021 gctgttggcg gtgatttcag tacgacgttt gtcaccgaac tgttcacgaa ccagctccag
     4081 ctcttcgcgg atcacttcca tcagacgatc ggcgctacca agaatacgca acagttccgc
     4141 gatctgatcc agcagctctt tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag
     4201 tttctgcaaa cgcagatcca gaatcgcctg agcttgctgt tcggtcaggt agtacagacc
     4261 atcacgcacg ccgaactctg gctccagcca ttccggacgc gcagcatcgt cgccagcacg
     4321 ttccaacatg gcggcaacgt tgcccagctg ccacggatta gcaaccagcg cagttttcgc
     4381 ttctgcaggc gtcggcgcat gacggatcag ttcgatgatc gggtcaatgt tcgccagcgc
     4441 cacggctaat gcttcaagga tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt
     4501 acgacgggtc accacttcac ggcggtgacg aacaaacgcc gcgatgatgt ctttcaggtt
     4561 catgatcttc ggctgaccat ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa
     4621 ctgggtctgg gagtagaggt tgttgagcac aacttcaccg accgcatcgc gtttcacttc
     4681 aatcacgatg cgcataccgt ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac
     4741 gcgtttttct tttaccagtt ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata
     4801 cggaatttcg tggacgataa tggtttcacg tccggttttg gcgtcaactt ccacttcagc
     4861 acgggcgcgg atatatacct tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg
     4921 accgttaatg attgccgccg tcgggaagtc cggccccggg atgtgttcca tcagcccttc
     4981 aatgctgatg tcttcatcat cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt
     5041 gtgcggcggg atgttggttg ccatacctac ggcgataccg gaagaaccgt tcaccagcag
     5101 gttaggaatt ttggttggca tgacgtcggg aattttttcc gtgccgtcat agttatcaac
     5161 gaaatcgacc gtctcttttt cgagatcggc catcagttca tgggcaattt tcgccagacg
     5221 gatttccgta taacgcattg ccgccgcaga gtcgccgtcg atggaaccga agttaccctg
     5281 accgtctacc agcatgtaac gcagcgagaa tggctgcgcc atacggacga tcgtgtcata
     5341 aaccgccgag tcaccatggg gatggtattt accgattacg tcaccaacga cacgggcaga
     5401 ttttttatag gctttgttcc agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg
     5461 gtgtaccggc ttcaggccat ctcggacatc tggcagcgca cggccaacaa tgaccgacat
     5521 cgcataatcc agataggagc tctttagctc ttcctcaatg ttgaccggtg taatttctct
     5581 cgcaaggtcg ctcatctaac cgctatccct ctactgtatc ccggattcaa aggtcgcaaa
     5641 ttataacaca gccgcgcagt ttgaggtaaa cctatacgct ttattcacat ccaatgcctg
     5701 atatactcgt ttgtcttgcc aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc
     5761 cggaaaacca taacgtagac catgaagaga tcgctaaatt tgaagccgtc gcctcccgct
     5821 ggtgggatct tgaaggtgag ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct
     5881 atattgccga gcgtgctggc ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg
     5941 gcggcattct ggccgagagt atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg
     6001 gctttgagcc attgcaggtg gcaaagctgc acgcgctgga aagcggcatt caggtggatt
     6061 acgtgcagga aaccgtggaa gagcacgcgg caaaacatgc cgggcagtat gatgtggtga
     6121 cctgcatgga gatgctggag cacgtacccg atccgcagtc agtggtcaga gcctgtgcgc
     6181 aactggtgaa accaggcggc gatgtctttt tctcgacgct taaccgcaac ggcaagtcat
     6241 ggctgatggc ggtggttggc gcggaatata ttttgcgcat ggtgcccaaa ggtacgcatg
     6301 atgtgaagaa gtttattaaa ccggcagagc tgcttggctg ggtggatcag accagtttaa
     6361 aagagcgaca tataaccggg ctacattaca acccgatcac taattccttt aagctcggcc
     6421 ccggcgtgga tgtgaactat atgctgcaca cgcagaataa gtgaggttga tgtttggccg
     6481 cgccaatgcc tgatgcgacg cttgcagcgt cttatcaggc ctacaaatgc tctccgtagg
     6541 ccagataagg cgtttacgcc gcatccggca attgtgatgg tcagacagcg attaccattt
     6601 caccgtcatc gacaaaaacc ctgccgtctg ggcaaaatca tcactccctt tctgccacgc
     6661 cacgctgccg cgcagagaca ctcgctgact gatattgccc gtgactccca cttttatttc
     6721 accccgtagc ttcaccgcat cgtcactgat agtgctgcca tcttcttcaa tttccgtcgc
     6781 atggggatcg tgataataat tcagatctaa tgttggtgtg acaccaaccg cggtacgcca
     6841 ttcgctgtgt aaacccagcc gcgtctgaat atcatccccc tgcgattgtg acacgcgcgc
     6901 atggttagcg gcggtaaaat catcctgctg cacgccctga taaatcacct gcgcctgcgg
     6961 ttcaatcacc acaccacgcc ccggtaacca ctgatacccg gcttccagcg aggcgataat
     7021 ccccgacgaa tggtaacgat ccacgccatc ttcatgttcg gaaacatcat tgttaaacca
     7081 cgcgtattgc agccagctat ccagccaggc cccctgcttc tgattaccgt gctgatacca
     7141 gcttgaggtc agcccaacgg cataaccgtg gttctggtta tcggcgcgag ttccggtcat
     7201 attcgagcgg ctgtcgccct gattatcact gtagccacca accgccccaa gcatccactc
     7261 gccatccgtg ccccagcacc cgctaaacaa gccgccgcta agcagcaccg tagaagtgtc
     7321 ttcctgctga gccagttgcc ctgccgccgt gtaatgataa cgtccgccga taacacgtaa
     7381 attcagcgtc tgaccatcgc caccagcgtg atcgcgtcgc tccatcataa acgcctgatt
     7441 tgccgcccgc aggttattga gataaccgcc aactttggca ttcagcaccg gctggtaagc
     7501 aggcgtaggg gccgggtcgg gggttgggtc aggatccggc gtgggatcag gatcgggagt
     7561 cgggtctggc tcaggtggcg atggcggcgt tacttcttgc gatcgcagat accagtcgtt
     7621 gttatcttcc actagcgtgt agtcatacgc tcccatgttg acgtagccac tgccagccag
     7681 actgaactgt gcattgtttt gaaactgcgt gggatcagct gcgaaatcaa ccactttaat
     7741 gcctgtcgat gtcggctcac caatccctgt aatggagtta accaccacag ttgtgttgcc
     7801 agcagtatta ccgttcatca ccaattgatc gcttaccgag tcatcgccgt ttaattcgct
     7861 gtcgaatagt agcgtaccgc cgccggtata atcaccgttt accgtcagcg tatcgccagc
     7921 gacgccattt tgcaggctaa ccgcaccgga cgtattggtc agatcaccat taaccgtcac
     7981 gtcagcaaga taggtcaggc tgtcagtttc aaggttggta ttaagctcgc ctgtcaacgt
     8041 cacgccatcg gtagcattta ccagcgtccc atcgccgtta aatagtgagg cattaagggc
     8101 gacggaagta tcctgcccgg tcagacgcaa aaccgtacca tcgctgatgt cgatagtgcc
     8161 gctggaagta gcatcaattg actgaatatc ctgatccgcg ccagtaacga acgttgcccc
     8221 atcaccaacc cacagtgacg aagcataagg aaagatatcc gcaacatcgc ctttcagggt
     8281 gccttcttcg acacgtaccg ccgactgcgt tgtaccgctg gcggtcagct ccagcgtacc
     8341 cgcccccgtt ttagtaaatg tgctaccctc atcctgatgc tgcccgctgc tgtcagccat
     8401 cagtgcgccc cactgcgtgt ctacccccgc atcaactgcc acttcaccgt cggcgcgcat
     8461 ttcaatatca cgcccatgct cagtcgtcgt agagcgagta tcgacattgg cagtaatctc
     8521 catcacgctt tcttgttact tatcggtaaa gataacctgg cggttatagt gggtatctcc
     8581 aagctgcgag ttgtcgctca ccatcagcgt accggaggcg atttgtgttg tgcccaaata
     8641 actattattg tttgccagac ttacctgccc gtcgctcgca tcgaccacca cgtagacgcc
     8701 atctttcccg tcaccgccga tatgcaaatt gtcgccacct gcaagatcta ccacatcctg
     8761 actgccgata accgttccgc tgctgccact gacttcaagg ccatcattgt atgatgtgcc
     8821 gctctgccaa gtggagaaat cagagagatc gagcacaccg ccgtttaaca cgatcgactg
     8881 cggatcgtcc tggagagcgg taagatctgc cgcgtcgcct tccagcgaaa gcaccgcacc
     8941 atcatcaacg actatatcgc cggttagcgc catcgactgc gcccctgcca gcacgtagct
     9001 gccgttttgc gcaatggtga gctgaccagc accttcgatg atgccagcaa aactgccctg
     9061 attaacagta acgttgccac cagcatcgat atttaaagtg ccattctgaa agcccgtcaa
     9121 tgcgtgcaca aaagtttgtt gggtcgagcc aacgtttagc tcagcctgat tctgatactg
     9181 atcaatactc cctatcgtca gaccgtagca gtcttgcgga tcgtcctggc aatgcgtatc
     9241 gccgacattc atcagggagt tgctgcgacc cagggtaact tcaccgtttt caatctgcat
     9301 ctcaccagta aagtcattgt tatctgcatt aagtaccaga tcgccggaac ctgttttggt
     9361 gattaacccg gtaccagcaa tagagtcaac agctccgtca ttctctgtat tgccgataac
     9421 cagcgttttt ccgtcggcaa tatcaaaggt aacttcactt aagccgagat acataaagcc
     9481 acccgccgca gtggaaggac catctccata gcctgctgcg ctattgttct catcgactaa
     9541 cacgccgccg ttctggctgt agctgtcatc aacagaaata tcaataagat agggagccct
     9601 cacgctattg gtatatatcg ccccgccata accttcggct gtgttatttg taaaggcggt
     9661 gttatttatt atcgtataac ctgaaggatg cttgctgtcg ctattattat cggtaacatc
     9721 gattgccccg ccatcgccat cactgtaact tgttgatgta tatgcctggt tattgttaaa
     9781 aacatcatca cttaaataga cgtcattatt aatggtataa attgcgccac ctttgccatc
     9841 attagcgata ttattgcgaa acatggcgtt agtgacacgt aaatcgacgg caccagtatc
     9901 attagtacca gaagaataga ttgcgccact atagccgcct gcgacgttac cggaaaaaat
     9961 aacatcagta agattcagcg ttgagttttc tttagcaaat atggccccgc cgttattata
    10021 ttctcctgta acggtgttat tggcaaacag agtcattcca gtttcatttt ctggtaatag
    10081 tgaaaattcc gctccttgtt gcaaaaatac ggccccaccg ctggcattat tggtcatatc
    10141 cgaaaaaacc agccattgcc catcgg
//
LOCUS       DADLTI010000142         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLTI010000142 DADLTI010000000
VERSION     DADLTI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.142"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..336)
                     /locus_tag="JN755_003272"
     CDS             complement(<1..336)
                     /locus_tag="JN755_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7381968.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="JN755_003273"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="JN755_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="JN755_003274"
     CDS             complement(1130..1336)
                     /locus_tag="JN755_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7381969.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="JN755_003275"
     CDS             complement(1443..2006)
                     /locus_tag="JN755_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7381970.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="JN755_003276"
     CDS             complement(3381..5216)
                     /locus_tag="JN755_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381971.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="JN755_003277"
     CDS             5317..5604
                     /locus_tag="JN755_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7381972.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="JN755_003278"
     CDS             5576..7105
                     /locus_tag="JN755_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY7381973.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="JN755_003279"
     CDS             complement(7275..8492)
                     /locus_tag="JN755_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7381974.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="JN755_003280"
     CDS             complement(8859..9845)
                     /locus_tag="JN755_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7381975.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3138 a         2262 c         1846 g         2709 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgcaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataaccagc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcgta ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcaacggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acaaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttc
//
LOCUS       DADLTI010000143         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLTI010000143 DADLTI010000000
VERSION     DADLTI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.143"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN755_003281"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN755_003281"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7381976.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN755_003282"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN755_003282"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7381977.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JN755_003283"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JN755_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JN755_003284"
     CDS             3456..5216
                     /locus_tag="JN755_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY7381978.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICMVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JN755_003285"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JN755_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7381979.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JN755_003286"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JN755_003286"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7381980.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JN755_003287"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JN755_003287"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7381981.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2557 a         2495 c         2372 g         2531 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcatggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt caaaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADLTI010000144         9798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLTI010000144 DADLTI010000000
VERSION     DADLTI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.144"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1063)
                     /locus_tag="JN755_003288"
     CDS             complement(<1..1063)
                     /locus_tag="JN755_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7381982.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGD"
     gene            complement(1642..2319)
                     /gene="bioD"
                     /locus_tag="JN755_003289"
     CDS             complement(1642..2319)
                     /gene="bioD"
                     /locus_tag="JN755_003289"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7381983.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(2312..3067)
                     /gene="bioC"
                     /locus_tag="JN755_003290"
     CDS             complement(2312..3067)
                     /gene="bioC"
                     /locus_tag="JN755_003290"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7381984.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(3054..4208)
                     /gene="bioF"
                     /locus_tag="JN755_003291"
     CDS             complement(3054..4208)
                     /gene="bioF"
                     /locus_tag="JN755_003291"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7381985.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(4205..5245)
                     /gene="bioB"
                     /locus_tag="JN755_003292"
     CDS             complement(4205..5245)
                     /gene="bioB"
                     /locus_tag="JN755_003292"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7381986.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            5332..6621
                     /gene="bioA"
                     /locus_tag="JN755_003293"
     CDS             5332..6621
                     /gene="bioA"
                     /locus_tag="JN755_003293"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7381987.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            6599..7069
                     /locus_tag="JN755_003294"
     CDS             6599..7069
                     /locus_tag="JN755_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7381988.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            7384..7467
                     /locus_tag="JN755_003295"
                     /pseudo
     CDS             7384..7467
                     /locus_tag="JN755_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            7623..8624
                     /locus_tag="JN755_003296"
     CDS             7623..8624
                     /locus_tag="JN755_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7381989.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(8658..>9798)
                     /locus_tag="JN755_003297"
     CDS             complement(8658..>9798)
                     /locus_tag="JN755_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY7381990.1"
                     /translation="YDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFD
                     NVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADE
                     SFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGS
                     TGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNT
                     AANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGV
                     SVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLT
                     YRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         2322 a         2564 c         2428 g         2484 t
ORIGIN      
        1 ggtcaccgcg atacatgccg ccaatttgtg gaatggtgac gtgagattca tcgacgacca
       61 gcagcccatc ggcaggcagg taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac
      121 cggagaggaa gcgcgagtag ttttcaatcc ccgaacagta gcccagctcg ttcatcatct
      181 ccagatcaaa ctgggtacgc tgggtcagcc gctgctcttc cagcagtttg ttgttttcca
      241 acagcacttt gcgtctggcg gccagttctt ctttgatctc ctccatcgcc tgtacgatgc
      301 gctcgcgcgg tgtgacgtag tgcgttttcg ggtagatggt aaaacgtgga atagtggaaa
      361 caatctgccc ggtcagcggg tcaaataacg acaatcgttc cacttcctcg tcaaacagtt
      421 ccacgcgaag tgcaatgtca tccgattctg ccgggaagat atctatcacc tcgccacgaa
      481 cgcggaaagt accacgctgg aatgcttgat cattacgagc gtattgcagc tccgccagtc
      541 ggcgcagaat cgcgcgctga tcgataatca taccgaccgt gagatggagc atcatcttga
      601 gatataaatc aggatcgccc agaccataaa tcgcggaaac agacgccact acaatcacat
      661 cacgccgctc cagcatcgct ttggtggcgg acaaacgcat ctgctcaata tgttcgttaa
      721 ccgaggcatc tttctcaatg aaggtgtcgg aactcggtac ataggcttcc ggctgatagt
      781 agtcgtagta ggagacgaaa tactccaccg cgttttccgg gaagaactct ttcatttcgc
      841 catacagctg ggccgccagc gttttgttgg gcgcaagtac catggttggg cgctgaaggt
      901 cagcaatgac attggcaatg gtgaaggttt tcccggagcc agtcacgcca agtaacgtct
      961 ggtgcgccag gccatcttcc agcccctctt cgagacgtcg aatcgcctct ggctgatcgc
     1021 cagaaggttt aaaagcggaa ttcagtttga acggtttact catgagtcgc tacctgaagg
     1081 agttgggcgg gcaggtatgt aattttactc gtcgtactta attatgccaa caaattatac
     1141 tggataaaaa aacagttcat caccataata tttctgatac agcgtaaact ccgctgtcaa
     1201 tcatgagcaa aatttactct gtggcgagat aaaactccgg ccttaccggg ttatccccaa
     1261 agcaacggct tttttaacat ttgtcaagat gagtgatgac agttttatgg caagagatgc
     1321 ctgtacagtg actgccattg catttatcta accagttaaa aaataaaaga tatttctttg
     1381 agccgtcttt aacgccaatt cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt
     1441 ctaactctaa tacacatggt tatccacagg aatagtggat aactgtctcc agcccctatc
     1501 ccccgccgcc tgggcaattc ccacattcgc cgcatgaggc ggaagtaaaa tttttttcgt
     1561 gattatttta gaatttaatt ggttaaattg cagtcaatcg aagacgcaat ctcgctcgca
     1621 atttaaccaa atacagaatg gctacaacaa ggcaaggttt atgtactttc cggttgccgc
     1681 attttctgga ttttctgcaa gccaggggat ctctcccagc agcggcgcgg gaatcatgcg
     1741 ggtgagcgtg gtcatatatt cagcgtgacg ttttcccgga ggcgtaacat cgttcgccac
     1801 ccaacccgcc agagtcagtc cggcgtgttg tattacctgc gcagtcaaca tcgcgtgatt
     1861 aatacagccg agtttcacac caactaccag tatcaccggc agttgttcct gtgttaccca
     1921 atctgcaaaa gtgaaagtgt cagaaagcgg cgtaaaccag ccgccagcac cttccactaa
     1981 cacccagtca gcctgttgtt caagcgcgcg taatccggcg ctcattacca atgattctat
     2041 cggcctgccc tcttgggcgc tgatgatgtg cggcgaggtg ggttctgcga aggtgtaagg
     2101 atttaccgtt gcgtaatcca gctgcaggct gctgttgtgc tgtaacgcca gcgcgtcact
     2161 attacgtaag ccttccgggg tcttttcgct gccagaggcg accggtttat aacctgccgt
     2221 ccgataacct actgccttcg cggcttgtaa aagtgcacaa ctggcgacgg ttttccccac
     2281 ttcggtatcc gttccggtga caaaataacg tttactcacg agcaatcact cccaaaaaaa
     2341 gatgatacgt cagaggatat cgcccctgtt gttgcggcca ggccagttgc aatcgctgca
     2401 actgcgaacg cgttaatatt cgcgggtcgc gcccttcatg aagatgcgta gcaccgatgc
     2461 ctttcagcga acgcatggca ctgagcgcat catcaaacca tagtgtgatg agctgaatat
     2521 gatgttgata acgcacgccg ttcagcgact cttcgatttc atctggcggt aaaaagcgat
     2581 tagcatgcgg acgctcgtcc accgcctgcc gcgcctgatg cagttcgggt aacgatccct
     2641 gcaccagcgt ggtaaacgcg accacgcctc cggggcgcac cacccgatac agctcgcgga
     2701 gtgccgtgga taaattaccg caccactgca ccgcgagatt gctccatgca agatcgaacg
     2761 tcgcagtcgc taacggcagg gattcgatat ctcccgccag ataatgatct gcggcatctt
     2821 tatggcgggc ttgcgcaagc attggcggcg agagatctaa cgccgtcacc tgcgcgtgac
     2881 gttcccgcca gtggcggctc atccagccag gtccacaacc cgcgtccagt acgtgggtgt
     2941 atttacgctg tggaagcatt gccagtaagg cgtcagcact ctggcgctgt agatctgcat
     3001 gttgctcata gtgtgcggct gcccgaccaa atgccgctgc aatggcttgt ttattaaccg
     3061 ttgccatgca gcacctccag cagacggtcg atatcctgca tttcatgcgc agcggttagc
     3121 gttaagcgaa gtcgcgcagt accagcgggt acggttggcg ggcgaatcgc cgtgacccag
     3181 cagccttgct ggcgcagttt ttctgccagt tgtaacgcac ggctgttatc accgacgatc
     3241 aatggctgga tggcgctgca tgaatcagcc agcgtaaacg gcaaatcctg tactccggca
     3301 cgaaaacgcg caatgagtga caccagtttt tcgcgccgtg catccccctc atcacggcga
     3361 atgaccgcca gcgacgcacg taatgcctgc gcctgagcgg gcggcatact ggtgctgtag
     3421 ataaggtggc gggcgaattg cagcagataa tccgccaccg tacttgagca aagcaccgca
     3481 gccccactga cgccaaatcc tttgccaaaa gtcactacca gcaattctgg ttttaccttt
     3541 tgcagccagc agctgccgcg cccctgctcc ccgataacgc ccgtgccgtg ggcatcatcg
     3601 accatcaacc agccattgtg ctgttgcgtt acctgctgga tttccgccag tggcgcgcta
     3661 tcgccgtcca tgctgaacac gccttctgtc accaccagtt gctgccccgg acagggggaa
     3721 gcaagcagtc gcgccagatg agcgacatcg ttatgagtaa aacggcgaag ctgcgacggg
     3781 cttaaactgg cagcctccag caatgaggca tggctaagcc ggtcggcaac aatacggtcc
     3841 tctttcgcca tcatcgcggc aatgactgcc tgattcgcgg caaaaccaga gataaacagc
     3901 agcgcccgcg aatagccaag ccactcggcc agttcttctt ccagcgcctg atgcaccacg
     3961 ctataaccgc tgacgtgacc ggagccgccg ctaccgatgc caaattgctc cgccccctgc
     4021 ttccaggcac ggataatttg cggatgatgg cttaaaccta aataatcgtt actggaaaag
     4081 ttcagatact ggcgatcatc cgccaccagc cagcgtccgg ctccttgcgc caccggataa
     4141 cggcgacgca gggcatcggc agcacgccgc gcatcgagcg ccgcgttgat tttctcctgc
     4201 cagctcataa tgctgccgcg ttgtaatatt cgtcggtgtc cggggtcatc agcgcctgtc
     4261 caagacgttg ctgttgttcg ttatcccctg ccagcacggc agtttgctgc ggatttagcc
     4321 ccagtttgcg gaacagttgc aggtctttat cttcttccgg attcggcgtg gtcagcagtt
     4381 tgcaaccgta gaaaatcgag tttgcgcctg ccataaagca catcgcctga gtctgttcgt
     4441 tcatctgctc gcgtccggca gaaaggcgca cgtaagaggt cggcatcatg atccgcgcta
     4501 cggcaatggt gcgaataaaa tcaaaggcat caacgtcatc gttatcggca agcggcgtgc
     4561 ctttcacctt caccagcatg ttgattggta cgctttccgg cggcgtcggc aggtttgcca
     4621 gttgcagcaa taatccggcg cgatctttta ccgtttcgcc taagcccaca atgccacctg
     4681 agcagacttt gatcccggca tcgcgcactt tttccagcgt atcgaggcgt tcctgataag
     4741 tgcgtgtggt gatgatattg ccgtaaaact ccggcgaggt gtccaggttg tggttgtagt
     4801 aatccagccc ggcgttcgcg aggcgctgcg cctgagattc actcaacgtg cccagcgtca
     4861 tacacgcctc cagccccatc gcttttaccc cctgcaccat ttgttccagg tacggcatat
     4921 cgcgttcgtg gggattcttc catgccgcgc ccatacagaa gcgcgtcgat cctgccgctt
     4981 tcgctttgcg cgccgactcc agcacctgtt caacttccat caaccgctcg gcttccagcc
     5041 cggttttgta gcgcgagctt tgcgggcagt atttgcaatc ttccggacaa gctccggtct
     5101 taatcgacag caacgtgctg acctgcacct gacgaggatc gaaatgctgg cgatgcacct
     5161 gctgcgcttc aaacagcaga tccagcaacg gtttttcaaa taattctgtg acttgcgaca
     5221 atgtccagcg tgggcggtga gccatggggc ttctccaaaa cgtgtttttt gttgttaatt
     5281 cggtgtaggc ttgtaaacct aaatcttttc aatttggttt acaagtcgat tatgacaacg
     5341 gacgatcttg cctttgacca acgccatatc tggcacccat acacatccat gacctcccct
     5401 ctgccggttt atccggtggc gagcgccgaa ggttgcgagc taattttgtc tgacggcaga
     5461 cgcctggttg acggtatgtc gtcctggtgg gcggcgatcc acggctacaa tcacccgcag
     5521 cttaatgcgg cgatgaagac gcaaattgat gccatgtcgc atgtgatgtt tggcggtatc
     5581 acccatgcgc ccgccatcga gctatgccgc aaactggtgg caatgacgcc gcaaccgctg
     5641 gagtgcgttt ttctcgcgga ctccggttcc gtagcggtgg aagtggcgat gaaaatggcg
     5701 ttgcagtact ggcaagccaa aggcgaagcg cgccagcgtt ttctgacctt ccgcaatggt
     5761 tatcatggcg atacctttgg cgcgatgtcg gtgtgcgatc cggacaactc aatgcacagc
     5821 ctgtggaaag gctatctgcc agaaaacttg tttgctcccg ccccgcaaag ccgcatggat
     5881 ggtgaatggg atgaacgcga tatggttggt tttgcccgcc tgatggcggc gcatcgtcat
     5941 gaaatcgcag cggtgatcat tgagccgatt gtccagggcg caggcgggat gcgcatgtac
     6001 catccggaat ggttaaaacg aatccgcaaa atgtgcgatc gcgaaggtat cttgctgatt
     6061 gccgacgaga tcgccaccgg atttggtcgt accggcaaac tgtttgcctg tgaatatgca
     6121 gaaatcgcgc cggacatttt gtgcctcggt aaagccttaa ccggcggcac aatgaccctt
     6181 tccgccacac ttaccacgcg cgaggttgca gaaaccatca gtaacggcga agccggctgc
     6241 tttatgcatg ggccaacttt tatgggcaat ccgctggcct gcgcggcagc aaacgccagc
     6301 ctggcgatta tcgaatccgg cgaatggcag cagcaggtgg cggctattga agtgcagctg
     6361 cgcgagcaac tggcaccagc ccgtgatgcc gaaatggttg ccgatgtgcg cgtactgggg
     6421 gcaatcggtg tggtcgaaac cactcgtccg gtgaatatgg cggcgctgca aaaattcttt
     6481 gtcgaacagg gggtatggat ccggcctttt ggcaaactga tttacctgat gccgccctat
     6541 attattctcc cgcaacagtt gcagcgtctg accgcagcgg ttaaccgcgc ggtacaggat
     6601 gaaacatttt tttgccaata atggcgataa gttgccgcat cgtcatgtct ttaacggcat
     6661 gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc ctgcgggaac
     6721 gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg gctggtggca
     6781 ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaagggt ttggctctgg
     6841 tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta aaaccggata
     6901 cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg ttcacgcgct
     6961 ggatgtagag cgtattgatg tcgatgaagg aggcagcggc gcgatggtcg ggtttaacgt
     7021 tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat cactctgcca
     7081 gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt tgtcttttta
     7141 ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg agaaacataa aacgctgtaa
     7201 atatcaatat gttatgaaag atttagtcta aataatagac tgcataatac tacaaaacac
     7261 aacatatcca gtcactatga atcaactact tagatagtat tagtgacctg aggcagagca
     7321 ttagcgcaag gtgatttttt gtcctcttgc gctaattttt tgtcaacgga ctgggttagt
     7381 tgaatttaca accatgctaa gtatctcgat aagcgcagag aaatgatgca gtggtgggcg
     7441 gattggcttg atgagaaggt taggtaggca aatccatgcc tgagcagagc cttgccctct
     7501 acctgagccc atgctcattc tttggcctca acgcgagcat gacctcactg gctggaccca
     7561 aaatggacta tgacagaaag atctgatatt tttcctcgca aaaacttaga ggctttattt
     7621 taatgcataa gagcaactgc tttgacacgg caaggcttgt ggcagctatg atggttctgg
     7681 tcagtcacca ctacgcgctc tctgggcagc cggagccgta cctgttcggc tttgaaagtg
     7741 ctggcggcat tgcagttatc atattctttt caatatcagg atatctgatt tcaaaatcag
     7801 ctatcaggag tgattccttt attgatttca tggctaaaag agcgagaaga atattcccgg
     7861 cgctggtccc ttgctctata ctgacatatt ttttatttgg atggatactt aacgacttca
     7921 gcgcagaata ttttagccat gacattgtca ggaagaccat aagctctatt tttatgagtc
     7981 aggctccaga tgctgacatt acatcacatt taattcatgc cggaattaac ggcagtttgt
     8041 ggaccctacc gcttgagttc ctgtgttata taataacggg agtagcagtt gccctactaa
     8101 agaacggaaa agcattcata gttatactgt tggtttttgt ttctctttca ttaattggaa
     8161 gtgtgtcaga aaacagggat gtaatgttct ctattccgtt atggctgtat cctttgcgtg
     8221 gactggcgtt cttttttggt gccactatgg caatgtatga gaagtcatgg aatgtaagta
     8281 atgttaaaat tactgtagtt tctttgcttg ctatgtacgc ctacgctagt tatggcaaag
     8341 gcattgatta tactatgact tgctatatcc tggtttcatt ctcgacaata gccatatgca
     8401 catccgttgg cgacccactg gttaagggca ggtttgatta ctcgtatggt gtgtacattt
     8461 atgcattccc ggttcagcag gttgttataa acacattgca catgggtttt tacccgtcaa
     8521 tgttgttaag tgccgtgact gttcttttct tgtcccatct ttcatggaat ctggttgaaa
     8581 aaagattcct gacacgaagt tcgcctaaat tatccctgga ttgaacaata aaaggggcca
     8641 tatggcccct aaaataatca gatggccaga tactcaacca ttgaaggcag cgcgactcct
     8701 actgggcttg ggcgagtaac attaattgtc accacggaaa cactaacctt gtcaccactt
     8761 gatgcagggt caccaaaaag gcttcttact ctgtatgtca ggttccccgg gttcggtata
     8821 gctgcagatc cgcttgtggc acctaccgca actgggacat taatgatccc atttacagac
     8881 ggtacgcctg aacagccacg atacgtattg tatctgcctt ccggtttctg cacctcggta
     8941 atgccagcca aataacagtc agtaactgac acgccaccgc tacgaagata gcgtagaggc
     9001 ttattgaata tgcacttatc gaaagaaaca cattcattga tgtcgacaat atttacagcc
     9061 cattcatcac cagttaacgt acaaccactt acaaagacga agatcgccga ctgttctatc
     9121 gctatacttt gattgtttgc agccgtgttt ttgtttgcag tgttgatatg aacaccactg
     9181 acaattttat gtgactgcgt tgaggtcggc gttctaaaat atatccccgt aaattgttca
     9241 gtgccatcgg catctgctgc tatccatgag tttgagaaag aaaatcctcc gtttacattc
     9301 tctataacca cgcctgttga accgcaataa tctaaatcca gttgttccat atgcaaatca
     9361 aggcaatcat gaacatagac ggcattttta aaaccaatgc acatggtctc actgtctaaa
     9421 attattgctt ctgacctaac tgcctctgac gaccacgcat acgtttgagg ctgaatcctg
     9481 atgccaaagc tttcatcagc agaataattt cctctgctga aatggcagga gctgacacta
     9541 acacttacgc actggcctaa aaagtgaaga ccgactctac aggttccagc ctgacatgct
     9601 ataaattttg agtgccagag cccttgcatt aaataagcgg tttcaacttc attcaccatt
     9661 acattatcaa atacccagcc ataaacctgt ctgatgacag agtcttctgc tgtaatgttt
     9721 gtgtcaatac cattgcaggt gttattacca gttactcgta tatttctcag ataacggccg
     9781 atagtgtctg aatcataa
//
LOCUS       DADLTI010000145         9771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLTI010000145 DADLTI010000000
VERSION     DADLTI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.145"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            49..579
                     /locus_tag="JN755_003298"
     CDS             49..579
                     /locus_tag="JN755_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7381991.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN755_003299"
                     /pseudo
     CDS             597..1235
                     /locus_tag="JN755_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN755_003300"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN755_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7381992.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN755_003301"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN755_003301"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7381993.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN755_003302"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN755_003302"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7381994.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN755_003303"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN755_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7381995.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN755_003304"
     CDS             complement(5552..6613)
                     /locus_tag="JN755_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7381996.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN755_003305"
     CDS             complement(6610..8007)
                     /locus_tag="JN755_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7381997.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN755_003306"
     CDS             8163..9161
                     /locus_tag="JN755_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7381998.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..>9771)
                     /gene="ompG"
                     /locus_tag="JN755_003307"
     CDS             complement(9272..>9771)
                     /gene="ompG"
                     /locus_tag="JN755_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7381999.1"
                     /translation="PDWDVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVETETGLQY
                     TFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDRW
                     SNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVG
                     VNYSF"
BASE COUNT         2473 a         2598 c         2344 g         2356 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtaacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc g
//
LOCUS       DADLTI010000146         9708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLTI010000146 DADLTI010000000
VERSION     DADLTI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.146"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(329..673)
                     /locus_tag="JN755_003308"
     CDS             complement(329..673)
                     /locus_tag="JN755_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7382000.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JN755_003309"
     CDS             805..2715
                     /locus_tag="JN755_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7382001.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JN755_003310"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JN755_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7382002.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JN755_003311"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JN755_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7382003.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JN755_003312"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JN755_003312"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7382004.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JN755_003313"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JN755_003313"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7382005.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JN755_003314"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JN755_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7382006.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JN755_003315"
     CDS             complement(8251..8853)
                     /locus_tag="JN755_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY7382007.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..>9708)
                     /locus_tag="JN755_003316"
     CDS             complement(9210..>9708)
                     /locus_tag="JN755_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7382008.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQD
                     VKKAV"
BASE COUNT         2371 a         2367 c         2559 g         2411 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgac gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaac
//
LOCUS       DADLTI010000147         9678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLTI010000147 DADLTI010000000
VERSION     DADLTI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.147"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            383..1501
                     /locus_tag="JN755_003317"
                     /pseudo
     CDS             383..1501
                     /locus_tag="JN755_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1567..1815
                     /locus_tag="JN755_003318"
     CDS             1567..1815
                     /locus_tag="JN755_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7382009.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="JN755_003319"
     CDS             1880..2248
                     /locus_tag="JN755_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7382010.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="JN755_003320"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="JN755_003320"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7382011.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /locus_tag="JN755_003321"
     CDS             3103..4350
                     /locus_tag="JN755_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7382012.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN755_003322"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN755_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7382013.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9030)
                     /gene="cusA"
                     /locus_tag="JN755_003323"
                     /pseudo
     CDS             complement(5895..9030)
                     /gene="cusA"
                     /locus_tag="JN755_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9042..9583)
                     /locus_tag="JN755_003324"
                     /pseudo
     CDS             complement(9042..9583)
                     /locus_tag="JN755_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2376 a         2684 c         2339 g         2279 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actagcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tatatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccaag
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg cagggaatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 tcatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggggcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat
     8221 ctccgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact
     8281 ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac
     8341 catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc
     8401 cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat
     8461 aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat
     8521 ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg
     8581 cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg
     8641 tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag
     8701 cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga
     8761 gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt
     8821 ggttagcgga taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt
     8881 tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat
     8941 ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc
     9001 caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca
     9061 ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac
     9121 aggccgccgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct
     9181 tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta
     9241 atcacccgct gttcgttgcc ggtatcaatc agcgcctcgt cggcgttgtc gacttccaga
     9301 cgcagctgca gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg
     9361 gtgagtgttt tatccggtcg cgccggaacg gtgagggtaa actgcgaggc atctttcacc
     9421 agccaggcga tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt
     9481 cgcgattacg cgcaataccc ctgagaaccg ccatgaacaa gggtttcatg atttgtgatt
     9541 ttagtttagt acgcaatttg gtacacaaca ctattttcac ttcagcgggt agtcatcaaa
     9601 ctcaccataa cgagcatcgt tgatgatgta tgtgatcacc acaaatatca catctggact
     9661 agttccgtct tcatgcgc
//
LOCUS       DADLTI010000148         9556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLTI010000148 DADLTI010000000
VERSION     DADLTI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.148"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..746)
                     /locus_tag="JN755_003325"
                     /pseudo
     CDS             complement(1..746)
                     /locus_tag="JN755_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(790..1161)
                     /locus_tag="JN755_003326"
     CDS             complement(790..1161)
                     /locus_tag="JN755_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HAY7382014.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTTD"
     gene            1685..2659
                     /gene="hemB"
                     /locus_tag="JN755_003327"
     CDS             1685..2659
                     /gene="hemB"
                     /locus_tag="JN755_003327"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7382015.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2765..3616)
                     /gene="tauD"
                     /locus_tag="JN755_003328"
                     /pseudo
     CDS             complement(2765..3616)
                     /gene="tauD"
                     /locus_tag="JN755_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(3601..4440)
                     /gene="tauC"
                     /locus_tag="JN755_003329"
     CDS             complement(3601..4440)
                     /gene="tauC"
                     /locus_tag="JN755_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7382016.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(4437..5204)
                     /gene="tauB"
                     /locus_tag="JN755_003330"
     CDS             complement(4437..5204)
                     /gene="tauB"
                     /locus_tag="JN755_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7382017.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(5217..6143)
                     /gene="tauA"
                     /locus_tag="JN755_003331"
     CDS             complement(5217..6143)
                     /gene="tauA"
                     /locus_tag="JN755_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7382018.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(6410..6925)
                     /locus_tag="JN755_003332"
     CDS             complement(6410..6925)
                     /locus_tag="JN755_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382019.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            7118..7369
                     /locus_tag="JN755_003333"
     CDS             7118..7369
                     /locus_tag="JN755_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7382020.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7371..9464
                     /locus_tag="JN755_003334"
     CDS             7371..9464
                     /locus_tag="JN755_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7382021.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
BASE COUNT         2403 a         2475 c         2395 g         2283 t
ORIGIN      
        1 attgctttct ttcacggcca ctctgcctgt atgtgcccgt ttcgatggcg gtacagcagg
       61 ttttcgctca agcaacagcg cattctggcg catgatccgg taaacacgtt tggcattgat
      121 cgcaggcata ccatcaagtt ctgcctgtct gcgaagcagc gcccataccc gacgataacc
      181 atacgtgggc agctctccga taacatggtg tatacggaga agcacatccg tatcatcagt
      241 gtgacgactg cggcggccat ccatccagtc atcggttcgt ctgagaatga cgtgcaactg
      301 cgcacgcgac acccggagac aacggctgac taagcttact ccccatcccc gggcaataag
      361 ggcgcgtgcg ctatccactt ttttgcccgt ccatattcaa cggcttcttt gaggagttca
      421 ttttccatcg ttttcttgcc gagcaggcgc tggagttctt taatctgctt catggcggca
      481 gcaagttcag aggcaggaac aacctgttct ccggcggcca cagcagtaag acttccttcc
      541 tggtattgct tacgccagag aaataactgg ctggctgcta caccatgttg ccgggcaacg
      601 agggagaccg tcatccccgg ttcaaagctc tgctgaacaa ttgcgatctt ttcctgtgtg
      661 gtacgccgtc tgcgtttctc cggccctaag acatcaatca tctgttctcc aatgactagt
      721 ctaaaaacta gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag
      781 ggggccagtc taatcagttg tcatgcactc ggaataaatc ataccgtgaa tagtgctgtt
      841 tttgattatc agactattgg cgttggtatt ggtagaaata ccgtcgtcca gatagtaggt
      901 agaaatcacg ccgttaacgg tagcgttatt aatgaccggg tagatatcgc cgttgtaata
      961 gctatcagcg gcatagttgt tccagcctac ataaccatca tagtaaacgt catcagcata
     1021 ggtggcgtcg ttgtagtgat ggaaagtatt gtaagtttga ccagaaatat cgttggtcgc
     1081 tgcattagcc tgagaggtga tagccagagt gcaagccaat gctaattgtg atactacaag
     1141 tttctttttc caggagtgca tttgtcatcc ccctcaggga cgtaataagt tgatccatca
     1201 attatcaatg catagaaaac atattatgtt tcgcgctaac gattatgctg cgttacagac
     1261 gaatggttca attattcttt cccctaagtc cgaatgtgga catatataat ttatggagaa
     1321 aaacacgcat caaattgata aaaggacgat atttcagcat gttgcagata atgttatttt
     1381 tgatgcatac gcgttataaa tatcaaaaac acaaaccaga taaatatctt atcaatatac
     1441 aacaaagttt gaattattca atttatagca gcacaaccaa acgccaaaaa aataataacc
     1501 aagagctata aataaagatt aaaaatattt tcagtgaata tgaaattatt gctatttcta
     1561 ttacaaagat gtcctggtct ctctttcatt ctcttcttgt caacatcgcg acaactttcg
     1621 taaaacactc ctgccccgct tcaggtatac tgtgcacctt aattccacaa acatcaggca
     1681 gaccatgaca gacttaatcc aacgccctcg tcgcctgcgc aaatctcctg cgctgcgcgc
     1741 tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct ttgttgaaga
     1801 agaaattgac gactacaaag ccgttgaagc catgccaggc gtgatgcgca ttccagagaa
     1861 acatctggca cgcgaaattg aacgcatcgc caacgccggt atccgttccg taatgacttt
     1921 tggcatctct caccataccg atgaaaccgg cagcgacgcc tggcgggaag atggactggt
     1981 ggcgcgtatg tcgcgcatct gcaagcagac tgtgccagaa atgatcgtca tgtcagacac
     2041 ttgtttctgt gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga
     2101 caacgacgcg actctggaaa atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc
     2161 agacttcatc gccccttccg ccgcgatgga cggccaggta caggcgattc gtcaggcgct
     2221 ggacgctgca ggctttaaag atacggcgat tatgtcgtat tcgaccaagt tcgcctcttc
     2281 cttttatggc ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta
     2341 tcagatgaac ccaatgaacc gtcgcgaggc gattcgtgag tcactgctgg atgaagccca
     2401 gggcgcagac tgcctgatgg ttaaacctgc tggagcgtac ctcgacatcg tgcgtgagct
     2461 gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt acgcgatgat
     2521 taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg
     2581 ttcaattaag cgtgcgggtg cggatctgat tttcagctac tttgcgctgg atttggctga
     2641 gaagaagatt ctgcgttaat ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt
     2701 acggtgaacg ccccctcacc ctaaccctct ccccaagggg gcgagggaac cgtccacact
     2761 cgtatcaccc cgcccgataa aacggtttat ccccaaggat cgtcgcccga tgcataatcc
     2821 gccgctgtgg caggtaatcg gcattggcat agtgctgagt cacgcggtta tcccaaatcg
     2881 caatatcatt cggttgccag cgccaacgca cctgaaactc cggtttagtg atatgggcga
     2941 ataaaaagcc taacaaggct tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa
     3001 agccttcatt cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc accaccggat
     3061 gtagcaacgg aggatttttc gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt
     3121 tgcggtattt gtattccggg aatgatttac ggaaatcatg ctccgcacgc agccagctca
     3181 gcagctggcg gaagggaaca gagagcgcct cataggccgc aataccgctg gttcagagca
     3241 tatcaccgcc ggtcgaaggt aactctttag ctgccagaat cgcccctgcg ggtggcgttt
     3301 caataaatgt cacatcggta tgccagttgt cgttatccgg cggattatcg ttatgggtat
     3361 ccagcacgat gatctcgtca accccttcgg catgagggta aacagggtga atatgcagtt
     3421 cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt aatagcttga tcgcgcagaa
     3481 ataccacctg atggcgcagc accgcatggt aaagctgttc aaactgatta tcgcttaacg
     3541 ggcgcgtcag gtcggcaccc gaaatttgtg cgccgatatg cggccccagc ggggtaatgc
     3601 tcagacgttc acccattgta cttctccatg ccagggcgtc agacggcgct gtaacgcgcg
     3661 cagacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg ccagtaccac
     3721 gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc gtgtcacggc
     3781 aatcagctcc gccgctacca gcgtagacca gcccacaccc agaccaatgc gtaatccggt
     3841 gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg cacggctggc
     3901 acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc ctgccagcgc
     3961 cgacatcgcc accggcgcaa aaatcgctaa atagatcagt aagatcttcg aggtttcacc
     4021 aataccaaac cagatcacca tcagcggcaa ataagccagc ggtggcaccg gacgataaag
     4081 ttcgattacc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg cgatcccgac
     4141 cggaataccg atcaccaccg ccgcgagtag cgcctgcaca atgcgcgtca ggctggctgc
     4201 cagatgctgc cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg tgagcagttt
     4261 tgccagtact tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca gcgccgccac
     4321 cgtccaccat acagtgagta aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga
     4381 gagcggccag cgccatttca gccgccgcga atgcagtttt tcattaatga gcacactcat
     4441 gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat
     4501 tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg agcaaagttg
     4561 agcggcagcc gctccagcac acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc
     4621 ataaacaccg cttcttctat atcgtgggta atcaacagca cctgcttgcc cgtctcctgc
     4681 cagagtttca gcagcagagt ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac
     4741 ggttcatcga gtaataacag ctggggattc gccgccagcg cgcgggcaat ccccacccgc
     4801 tgacgttgac catcggaaag ctgccagata tagcgttttt ctgcgccttc cagccccact
     4861 tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc tgccaattgc
     4921 aggccgaacg ccacgttgtc ctgtacattg cgccacggta gcagcccttc attctgaaaa
     4981 actacgccac gctctgctcc cggtccctca atacgcttac ccgccagttg aatgctgcca
     5041 tgctgataag gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc gcagccggac
     5101 ggccccagca ccaccagtag ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc
     5161 ggtttgccgc cataatcggc atagagatga gagatttgca gcatctgcgc ctccttttat
     5221 tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg gaccttgccc
     5281 tgctctttca aaaactgcgc ggtgtcgatg attgctttgt tcaccggtcc ggtcagttct
     5341 gccgtttgtt gctgcggcgt cagataggta tttcccttca ccagccccgg aacgtcacct
     5401 tcaggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt cagccacgcg
     5461 tcagggttag caatgtacgg ttgctgtgca tcgatggcgc ttttggcgaa cgctttcacg
     5521 acctcaggat gtttctcggc aaaatctttg cgcaccaccc agacgtccag cgttggcgca
     5581 ccccactgcc cgacctgttc agaatcggtc agcaccttgt cgtctttttc cagggcgtta
     5641 accgccggtg cccagacata agcaccatca atatcacccc gctgccatgc ggcgataatc
     5701 gcgggcggct gcaggttcac aatctccact agccctggtt taatccccca gtgtttcagc
     5761 gccgccagca ggctgtagtg ggtggtggag ataaacggta cggcaatgcg tttaccaatc
     5821 aaatcttcgg gtttgctgat agttttcttt accaccagcg cttcggagtt acccagtttt
     5881 gacgccagca agaagacttc aatcggcacc tgttggctgg ctgcaaccgc taatgggctg
     5941 gatccgaggt tgccgatttg cacgtcgcca gaagccagcg cccgcacgat gctggctccg
     6001 ctgtcaaact tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt gttgtcggcc
     6061 tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt caccgcctgt
     6121 gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tatatgacta ggtaatttat
     6181 tcaagataac cgggaatggg taaaaaaaga cgggatttga tgggattcaa taagttatgc
     6241 gccgattgca ttcaagataa aacgagttaa aaatgctcaa tgcttcgata tcgataaaac
     6301 attcaagata ggcgcgccgt gtattttaat tcttttaaga ctcttcaaaa atgtcatcgt
     6361 agcctttaaa aacgaatttt aaaggcaccc taaaggcagg tcaatgaagt taacccgcct
     6421 tcgcgtttac actctctgag agaatactag cttcaatctc ttccaaaatc tctctgactt
     6481 gctcgtcggg catatcaacg agcatcaacg ctgccaggcg tttacggccc tctagttgca
     6541 gtagctcttg agtttcctta tctaacaagc gtagcacagt ctcaacacct ttccttgcga
     6601 gcatgtttgt aagggcttct cgttcctcac tggtaagaaa cgacaacatt tctgttagct
     6661 gatccccttg gcttaaatca ctttgtgttt tttgtgtttt tggtggattc ccgttaccgg
     6721 tttttagcca ttcaagacta acaccttcac tttctgcaat gcgtaccaat atgctttctc
     6781 gaggttctgg tgtatttccc tttttaaaat agctttgtaa cgtattgata tttatgccac
     6841 aggctctggc ggcagcactt cttgatggat accgcctaat aagataagcc atgcgctcca
     6901 cgataggttc ttttgcctgc tccataaaag tacctctagg tatttttctg tataaaggtt
     6961 cttttccatc tatctaattg aatggaaagg taattttaaa aatccaaata aaatacaaaa
     7021 aaataccttg tattggtgct ttttgtgatc tgcgatttta tttactcgga tatccacgcg
     7081 aatatccgcg cgggtaattt tgtagggtaa aggaaagatg agacaaagag aagatagtgg
     7141 ccgcgattgg ccttcgctga tgattcagca agcactcaaa gataatgggt ggtcttcctt
     7201 tcgggagctt tcactccatg ccggttatgc tcaggattca ctgaaagcgg tagttcgtac
     7261 gccaaacaaa aaatacgaac gtgttatttc tgacgctata ggtgtaccgc cagaaatcat
     7321 ctggcccagc cggtatcgta acgatggatt tatcaggaag gcgtcgtgat atgcagtcgt
     7381 acgttaccgt aaatgacctt ctcggtgttc ctggaatgcc agccaccgcg aaaggcatcc
     7441 gacaggcgtt acagcgtttt tctcgtgctc ttggtgacgt tagtcgccgt cgagcaggta
     7501 caaaagccat tgagtaccat atcgattgcc tgcctgaggt aacccaaaag gcactgcgtg
     7561 aacgctatgt ggaacaactg gtggctacag agaacaacgt ctccgaagtg aagaccgtta
     7621 ccaggacggc gcgtaaccct gatgctgttc aggcagttga agcatatcgg ggttctccgc
     7681 aactaatgga agaacgcctg aatgccctga ctgaaaatca gcgccgggtg tctgaagcaa
     7741 gagcggcgct ggcagtggaa gtgctgaagc tggaaagcgc cggtaacctc gggcgactga
     7801 aagccattaa ctttctggtt gaaaaagccc gtaaaggtga gttgccggaa cgactgcaac
     7861 aggctgcgat taacgctaat gcaaaacgtg gcactaatcg caccatcagc cgtgatccgc
     7921 tttaccagtg ggtactgaaa tacaaccggt cgcagaacgc cgcagaacga ctgttgttac
     7981 tggcaccagg aaaacgcgac gaaataaaac cggaagaaat tagctggctg cctgaatttc
     8041 tggcgcagta tcgtcaggct aacggcagac ctatgtcgga agcctacgag gattttgtca
     8101 cggaatggca acgtcgctat gcggacgaac cgtacatgct ggaggtgatg ccttcctatg
     8161 atgttgtccg gtatgccatg aaaaaattgc cggaagtggt gaaacaaaaa ggacgcgtta
     8221 ccggcagtga ataccgccag cttgagggat tcacccgccg cgactggaca gctatgccgg
     8281 tgaattatgt ctggattggt gacggccacg gtatgaagct gaaatgtgcg catccgatcc
     8341 acgggcgtcc attttcaccg gaagtcacgt ttgttatcga cggtggaacc cggtttgttg
     8401 ttggctggag tcttgatctt gcggagaacg tgtttgctgt tgccggggcg atacagcacg
     8461 gaattcgcaa tcacggaaaa cccttcctgt attactcgga taatggttcc ggtgaaacgg
     8521 cagatatgct ggataaagag atcgtcggga ttctgccccg tctgggtatt aaccacccca
     8581 ccggtattgc cggaaatccg cagggacgcg gcatcattga acgtctgaac cggacgctgc
     8641 cgatgcgtat agccagacgg tatcgcacct attttggcaa gggggcagac cgggaaacac
     8701 tgcgtgtact caatcgcgat ctgcgttcag catttaatgc cctgcaacag gacaaaccgc
     8761 tgaatgcccg ccagaaatct gcaatgcgcg agctgccgtc ctggtcagaa ctgattgagg
     8821 ctattcgtga aggagtggaa tggtacaaca accgcccgca ttctgaactg ccgatgaaac
     8881 caaacgggaa gcattacagc ccggctgagt tcagaaaaaa acgcctggcg gaagaaaaca
     8941 ccgaaattga atggctgagt gatctcgaac tgcgcgacat gttctgcccg atggtggagc
     9001 gtccggtaag acgctgtgaa atcaaatggc ttgggaatat ttattacgcg ccggagctgc
     9061 gtgatgaaca tggtcgcaaa gtgcttatca gctatgacat tcatgatgca gagcgaatta
     9121 cagtgcgccg taaggatggc agttttattt gtgaggcgat atggaacggc aataaacgcg
     9181 ccgccttccc ggtcagcgcc gactattaca aacaacagca gcgtattaaa ggaatgcaca
     9241 aacgcgcaga agaaaaaatc cgtgatgccg tggatgaagg tattcaggtt attgaacaaa
     9301 aaacgacaga accctggctg gataatgttt atcgacccgt tggcaatgtg atgaccgttc
     9361 agcaacagga atatgaagag gagcacgacg aagaatccga acgcaatttc aggcggggat
     9421 tacagaaact cattgccatg caggacgatg acccactggc ctgaataaaa aaacagtccg
     9481 cagcaacgga ctgttaaaca ggtaaataaa gctgatttga aataaccgag gaaacaatat
     9541 gaccgatatt aatgat
//
LOCUS       DADLTI010000149         9521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLTI010000149 DADLTI010000000
VERSION     DADLTI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.149"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(434..640)
                     /locus_tag="JN755_003335"
     CDS             complement(434..640)
                     /locus_tag="JN755_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7382022.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JN755_003336"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JN755_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7382023.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JN755_003337"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JN755_003337"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7382024.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JN755_003338"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JN755_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JN755_003339"
     CDS             4795..5367
                     /locus_tag="JN755_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7382025.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8039
                     /gene="kdbD"
                     /locus_tag="JN755_003340"
                     /pseudo
     CDS             5360..8039
                     /gene="kdbD"
                     /locus_tag="JN755_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8036..8713
                     /gene="kdpE"
                     /locus_tag="JN755_003341"
     CDS             8036..8713
                     /gene="kdpE"
                     /locus_tag="JN755_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7382026.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8988..9092
                     /locus_tag="JN755_003342"
     CDS             8988..9092
                     /locus_tag="JN755_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7382027.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9403..>9521
                     /locus_tag="JN755_003343"
     CDS             9403..>9521
                     /locus_tag="JN755_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7382028.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2115 a         2395 c         2637 g         2374 t
ORIGIN      
        1 ataacatctt attggattag atatgttaac aattaattat tcttaatata tcaataaagt
       61 atatattact ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg
      121 gaatttattt agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa
      181 gtttcacgaa agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc
      241 gcttagagcg acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga
      301 aacgtgatgc taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat
      361 tatcatggaa gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta
      421 agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg
      481 gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt
      541 tccagaaaaa catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta
      601 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
      661 tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
      721 ttttgtgtaa ttttaccact catccgacca cttatttttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttaatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatactt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctccgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 caccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctatt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caacgtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctggcg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaac cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcagaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctctac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaatgacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acggctggtg gtggtggagc
     7201 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acttttacgc
     7261 tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga
     7321 tggcaagcga acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac
     7381 gcacgccgct tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg
     7441 aaggatcacc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc
     7501 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
     7561 aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt
     7621 tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc
     7681 cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc
     7741 aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg
     7801 ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg
     7861 ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg
     7921 tggatgtaca cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc
     7981 gtgttacact tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac
     8041 aaacgttctg attgttgaag atgaacaggc tattcgtagc tttctgcgca cggcgctgga
     8101 gggcgacggg atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc
     8161 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga
     8221 gtttatccgc gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag
     8281 cgaagagagc gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
     8341 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac
     8401 cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt
     8461 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctagcggt
     8521 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     8581 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     8641 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg
     8701 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     8761 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8821 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8881 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8941 gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata
     9001 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata
     9061 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     9121 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     9181 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     9241 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     9301 tttatttatc agcggtggag ctttgctttt tttccggcgt gatcgatttc tcctttgaga
     9361 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     9421 gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     9481 gaaagtaatt atattgacgt tgccgccatt attttatcag t
//
LOCUS       DADLTI010000150         9497 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLTI010000150 DADLTI010000000
VERSION     DADLTI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.150"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JN755_003344"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JN755_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7382029.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..1526)
                     /locus_tag="JN755_003345"
     CDS             complement(444..1526)
                     /locus_tag="JN755_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7382030.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JN755_003346"
     CDS             complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JN755_003346"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7382031.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JN755_003347"
     CDS             complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JN755_003347"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7382032.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4460..5066)
                     /locus_tag="JN755_003348"
                     /pseudo
     CDS             complement(4460..5066)
                     /locus_tag="JN755_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5114..5860)
                     /locus_tag="JN755_003349"
                     /pseudo
     CDS             complement(5114..5860)
                     /locus_tag="JN755_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5891..6733)
                     /locus_tag="JN755_003350"
     CDS             complement(5891..6733)
                     /locus_tag="JN755_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7382033.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6720..7601)
                     /locus_tag="JN755_003351"
     CDS             complement(6720..7601)
                     /locus_tag="JN755_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7382034.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7622..8905)
                     /locus_tag="JN755_003352"
                     /pseudo
     CDS             complement(7622..8905)
                     /locus_tag="JN755_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8919..>9497)
                     /locus_tag="JN755_003353"
     CDS             complement(8919..>9497)
                     /locus_tag="JN755_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY7382035.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2266 a         2647 c         2324 g         2260 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
     1261 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
     1321 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
     1381 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
     1441 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
     1501 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
     1561 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
     1621 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
     1681 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
     1741 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
     1801 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
     1861 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
     1921 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
     1981 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
     2041 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
     2101 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
     2161 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
     2221 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
     2281 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
     2341 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
     2401 tgccactgct caggtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
     2461 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
     2521 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
     2581 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
     2641 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
     2701 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
     2761 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
     2821 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
     2881 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
     2941 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
     3001 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
     3061 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
     3121 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
     3181 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
     3241 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
     3301 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
     3361 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
     3421 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
     3481 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
     3541 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
     3601 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
     3661 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
     3721 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
     3781 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg
     3841 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
     3901 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
     3961 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
     4021 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
     4081 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
     4141 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
     4201 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
     4261 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
     4321 atccctcgcg cctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
     4381 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
     4441 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
     4501 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
     4561 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
     4621 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
     4681 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
     4741 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
     4801 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
     4861 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
     4921 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatccag ttctttattg
     4981 gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa
     5041 atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac
     5101 gatggtgaaa gtcgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5161 tggtttcgat aatcacatcg gccccctgct tgccggtcag cgttttgatc tctttgccta
     5221 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5281 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     5341 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     5401 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     5461 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     5521 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     5581 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     5641 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     5701 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     5761 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     5821 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     5881 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     5941 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6001 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6061 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgtcacc ataaagcagt
     6121 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6181 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6241 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6301 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     6361 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     6421 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     6481 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     6541 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     6601 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     6661 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     6721 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     6781 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     6841 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     6901 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaagaccgg cataattgcg
     6961 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7021 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7081 tagcgccaga tggcgagcag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7141 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7201 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7261 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7321 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     7381 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     7441 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     7501 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     7561 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     7621 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     7681 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     7741 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     7801 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     7861 aagtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     7921 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     7981 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8041 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8101 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8161 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8221 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8281 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     8341 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     8401 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgc
     8461 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     8521 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     8581 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     8641 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta taagcgtctt
     8701 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     8761 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     8821 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     8881 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     8941 caagggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9001 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9061 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9121 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9181 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9241 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9301 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
     9361 aacaaaatcg catgggcaag gataaacctg gcgcaacgtt cttcatcgct actctcgcgg
     9421 cggcttaacg catccatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
     9481 ttatttttcc agtttac
//
LOCUS       DADLTI010000151         9361 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLTI010000151 DADLTI010000000
VERSION     DADLTI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.151"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(3..79)
                     /locus_tag="JN755_003354"
     tRNA            complement(3..79)
                     /locus_tag="JN755_003354"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Arg,seq:acg)
     gene            complement(83..175)
                     /locus_tag="JN755_003355"
     tRNA            complement(83..175)
                     /locus_tag="JN755_003355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Ser,seq:gct)
     gene            complement(491..676)
                     /gene="csrA"
                     /locus_tag="JN755_003356"
     CDS             complement(491..676)
                     /gene="csrA"
                     /locus_tag="JN755_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7382036.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(911..3541)
                     /gene="alaS"
                     /locus_tag="JN755_003357"
     CDS             complement(911..3541)
                     /gene="alaS"
                     /locus_tag="JN755_003357"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7382037.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3669..4169)
                     /gene="recX"
                     /locus_tag="JN755_003358"
     CDS             complement(3669..4169)
                     /gene="recX"
                     /locus_tag="JN755_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7382038.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4412..5473)
                     /gene="recA"
                     /locus_tag="JN755_003359"
     CDS             complement(4412..5473)
                     /gene="recA"
                     /locus_tag="JN755_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7382039.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="JN755_003360"
     CDS             complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="JN755_003360"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7382040.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="JN755_003361"
     CDS             complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="JN755_003361"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7382041.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7536..8099
                     /gene="srlA"
                     /locus_tag="JN755_003362"
                     /gene_synonym="gutA"
     CDS             7536..8099
                     /gene="srlA"
                     /locus_tag="JN755_003362"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7382042.1"
                     /translation="MIETTTHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8096..9055
                     /gene="srlE"
                     /locus_tag="JN755_003363"
                     /gene_synonym="gutE"
     CDS             8096..9055
                     /gene="srlE"
                     /locus_tag="JN755_003363"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7382043.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9066..>9361
                     /gene="srlB"
                     /locus_tag="JN755_003364"
                     /gene_synonym="gutB"
     CDS             9066..>9361
                     /gene="srlB"
                     /locus_tag="JN755_003364"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7382044.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGT"
BASE COUNT         2151 a         2599 c         2291 g         2320 t
ORIGIN      
        1 gatggtgcat ccgggaggat tcgaacctcc gaccgttcgg ttcgtagccg agtactctat
       61 ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt
      121 ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg
      181 cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata
      241 ttactttctg agacttataa gtcaaacaat ttttcccaca cttttatcgt ttgcacactt
      301 cacgctcaat tagtctgtaa aaacgtcaaa aagagtgttt tatctacaga aaaacggagg
      361 tctggcagat agcagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag
      421 cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga
      481 cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct
      541 cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct
      601 ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac
      661 gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc
      721 ccggcattat ccatcatata acgccaaaaa gtaagcaacg acaaacacat tacatgtaag
      781 cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg
      841 actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct
      901 gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag
      961 gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc
     1021 cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt
     1081 ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa
     1141 ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa
     1201 caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa
     1261 gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact
     1321 gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca
     1381 gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt
     1441 ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc
     1501 ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga
     1561 aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg
     1621 cacctttcgc tttcgccgct tcgagattca tgatgttggt ttcgatcggc aaattgcgac
     1681 gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt
     1741 tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat
     1801 gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac
     1861 gaatacgggc gcgacgagcc tcatcaatat cagcctgcac agcgtcgccc actttcagag
     1921 aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca
     1981 ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt
     2041 ccgcatagaa tggcgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat
     2101 caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat
     2161 agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc
     2221 cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt
     2281 caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg
     2341 tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct
     2401 cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct
     2461 tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa
     2521 cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc
     2581 cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg
     2641 gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca
     2701 gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc
     2761 ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca
     2821 gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc
     2881 ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg
     2941 gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg
     3001 ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt
     3061 tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt
     3121 cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa
     3181 accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt
     3241 cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga
     3301 cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc
     3361 gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt
     3421 tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat
     3481 gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca
     3541 taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc
     3601 cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc
     3661 gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca
     3721 cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca
     3781 ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc
     3841 atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt
     3901 gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca
     3961 tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca
     4021 tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct
     4081 tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat
     4141 gccgggcgac gggatgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag
     4201 ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtatgcag
     4261 aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
     4321 gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
     4381 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc
     4441 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4501 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
     4561 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4621 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
     4681 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
     4741 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     4801 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     4861 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     4921 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     4981 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5041 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
     5101 gctcgccggt atccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
     5161 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
     5221 gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga
     5281 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5341 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5401 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5461 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5521 ttgaaacaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5581 aaggtctgca atacatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag
     5641 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
     5701 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
     5761 tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg ttcactcacc
     5821 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
     5881 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     5941 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
     6001 agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat cacaacctct
     6061 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6121 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6181 cctcttgtaa atagctactg tactcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6241 gccattgcgt aatgggtgct gtggttgtaa cgggtgatgg tgtagaagtt cggcagaccg
     6301 taccagtact ggtagccggt gccaacatcc agacgcagca ggctggcttg ttgatggttg
     6361 cccagcggct gctgtggcgt taaacctgcg gcggcaagct gcgaaatgct gtacttagtt
     6421 ttgaaaccat ttggcaagcc tggagcctga ccgtttgcca ttatcgcgac ctgatcgcct
     6481 ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc
     6541 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6601 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6661 tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
     6721 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     6781 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
     6841 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     6901 acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt cggacctgat
     6961 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7021 cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7081 attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
     7141 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7201 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7261 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7321 taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
     7381 ctcaactcat tcccctcgct atacttttat tcaaactttc aaattaaaat atttatcttt
     7441 cattttgcga tcaaaataac acttttaaac ctttcaacct gattagatta ggttgccgtt
     7501 tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccact actcatggtg
     7561 cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccgga atggtgaccg
     7621 gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg
     7681 gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc
     7741 tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta agcctaggtc
     7801 gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact
     7861 caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgtttat cttggcattg
     7921 ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagt tatctgctgg
     7981 ttggtctggt caccaatttc ttccgcggct gggtgaccga tctgaccacc gccatttttg
     8041 agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac
     8101 gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tggaagctac
     8161 gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact
     8221 ggcacaactt actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc
     8281 ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa
     8341 acgacgtatt cccaccatta atatccactc gacgggcaag tccggtccgc tggcgcagta
     8401 cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga
     8461 tgcgacacca caaccctctt ccgtgggccg tgactatgac accagtaaga aaatcaccga
     8521 acaaagcgat ggtttactgg cgaaggtggg aatgggcatg gggtccgccg ttgccgtgtt
     8581 gtttcagtct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc
     8641 attcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca
     8701 cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc tcatctgctc
     8761 cttcccactg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt
     8821 attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact
     8881 gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga
     8941 agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc gctttttaac
     9001 cggcgcgccg actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct
     9061 gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt gccactgacg
     9121 ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac ctcgaagagt
     9181 attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaatttt
     9241 cactcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa gacaaccttc
     9301 gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca
     9361 c
//
LOCUS       DADLTI010000152         9238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLTI010000152 DADLTI010000000
VERSION     DADLTI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.152"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN755_003365"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN755_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7382045.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN755_003366"
     CDS             1828..3147
                     /locus_tag="JN755_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7382046.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3512..4657
                     /locus_tag="JN755_003367"
     CDS             3512..4657
                     /locus_tag="JN755_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7382047.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JN755_003368"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JN755_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JN755_003369"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JN755_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="JN755_003370"
     CDS             5803..6699
                     /locus_tag="JN755_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7382048.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="JN755_003371"
     CDS             6696..7682
                     /locus_tag="JN755_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7382049.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JN755_003372"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JN755_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN755_003373"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN755_003373"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7382050.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2254 a         2255 c         2321 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatct
//
LOCUS       DADLTI010000153         9204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLTI010000153 DADLTI010000000
VERSION     DADLTI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.153"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..414)
                     /locus_tag="JN755_003374"
     CDS             complement(<1..414)
                     /locus_tag="JN755_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7382051.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMS"
     gene            complement(436..762)
                     /locus_tag="JN755_003375"
     CDS             complement(436..762)
                     /locus_tag="JN755_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7382052.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(981..1946)
                     /gene="glk"
                     /locus_tag="JN755_003376"
     CDS             complement(981..1946)
                     /gene="glk"
                     /locus_tag="JN755_003376"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7382053.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            2150..3406
                     /locus_tag="JN755_003377"
     CDS             2150..3406
                     /locus_tag="JN755_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7382054.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            3521..3847
                     /locus_tag="JN755_003378"
     CDS             3521..3847
                     /locus_tag="JN755_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7382055.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3988..5226)
                     /gene="mntH"
                     /locus_tag="JN755_003379"
     CDS             complement(3988..5226)
                     /gene="mntH"
                     /locus_tag="JN755_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7382056.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            5562..6764
                     /gene="nupC"
                     /locus_tag="JN755_003380"
     CDS             5562..6764
                     /gene="nupC"
                     /locus_tag="JN755_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7382057.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(6814..9003)
                     /locus_tag="JN755_003381"
                     /pseudo
     CDS             complement(6814..9003)
                     /locus_tag="JN755_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT         2386 a         2409 c         2193 g         2216 t
ORIGIN      
        1 agacatcaac ccgccgataa aaccagccgg gaacgccagt ttgccaccga tagagttcgc
       61 cacaaatgca gcaaacatcg gaatggcaaa accaaacaac acgccgccga aggactgcga
      121 cagccaggca aatttaagta aggagaggtc gaaaccggag aatttcccgc tattaagagc
      181 atccatgatg ccgatctcag caggaatttt aagccagcta taagcaatca gctggctgaa
      241 agcgaggatc acaccgccca taattaaggt cggcaccatg cgtgaaatcc ctgacatcac
      301 atgctgcggg agttcacccc agaagctgct tttagaggcc tgcggattct taactgccgc
      361 tgccgcaccg gatgcgccag gcacaacggt tgcactgcgt tttttaatgg ccataacaat
      421 tatccctgtc gaatattact gttgttcaga ggcaatcatc tcttcgattt ctttgatgat
      481 gcccgcagcg tttttaattg cgtcctgcaa agtgatttca taaacgtcgc gtgattcgaa
      541 acgttcgtta tcttccgggg taactgccac ggaatggatg atgatggtcg cttcggcgat
      601 atcctgcgcc gtcaggcgat tctggatgcc gtccgcgccc tgggtttcaa ttttcacttc
      661 ataaccggct tctaccgccg cttcttccag cgcctgagcg gccataaaag tgtgagccag
      721 gcccattggg caggcacata aggcaatcag tttcttactc atcgttaatt cctcgttcag
      781 tgatagttga cgagtgaaat tatgaacgtg caaaacaaat cgccgttacc agacaaataa
      841 tgcatttact ggagaattaa tcccgctgta cggaagtgtg acgcaggtcg gccttgtgag
      901 gggacacaaa gaaaaattga attaactgta tgatttaaaa tattatcggg agagttacct
      961 cccgatataa caggaaggat ttacagaatg tgacctaagg tctggcgtaa atgtgcaccg
     1021 gaaccgagaa gacccggata gtcatggacg atgagataca ccggaatatc atggacatat
     1081 tctttaaagc gccctttatc ttcaaatgcg gcacggaaac cggaggcttt gaagaactca
     1141 aggaagcgcg gcacgatacc gcccgcaata aacacgccgc caaatgtccc gagattgagc
     1201 gccagattgc cgccaaaacg gcccataatg acgcaaaaca gcgacaatgc gcggcggcaa
     1261 tcggtgcagc tgtcagccag cgcgcgttcg gtaatatctt ttggcttgag attttctggc
     1321 aggcggttgt cagctttcac aattgcgcga tacaaattca ccagcccagg gccagaaagc
     1381 acgcgctccg ccgaaacatg accaatttcc gcacgcaata tttcgaggat aatggcctct
     1441 tcttcactat tcggcgcaaa atcaacgtga ccgccttcgc ccggcaagct tacccaacgc
     1501 ttatcgacat ggaccagatg cgcaacccca agccccgttc cggcaccgta aaccgcaata
     1561 ggcttacctt cgaccggttc tgcgccacca aactgaatca gatgctcttt tttcagcatc
     1621 gggatcgcca tcgatacagc ggtaaaatcg ttaataattt ccagatggct aaaaccgaga
     1681 ttctttttca tttcggcaat tgagaacgcc caggtatggt tggtcattgc cacccagtca
     1741 ccggtaattg ggcaagcgat ggcaatacag ccgtctttca cctcgacctt atgttcttca
     1801 agataaacgc gaatgaccgc ttcgaggctg gggtaatcaa gccctgaata ggtcttagcc
     1861 tgcgagattt caccactggc aatatcacac agagcaagac gtgcgttggt gccgcccaca
     1921 tcaccgacta atgcatactt tgtcattctt caactgctcc gctaaagtca aaataattct
     1981 ttctcacact gtaaatactt gggggcataa caacaacgcc ggaaaggcag gctccctgta
     2041 aatatcgatc tgggtcacac aattacttta tcgtttcagc accaattgca gcgatgcctt
     2101 tttgcaagct gggcaaacta agtatctgac cccgcataag gaatagaaca tgctccatcc
     2161 gcgagccaga accatgttgt tattatcgct ccccgccgtg gcaattggga ttgcgtccag
     2221 tcttattctg attgtggtga tgaaaatcgc ctcggtatta cagaatttgc tctggcaacg
     2281 actgccggga actctgggga tagcccagga ttcccccctc tggatcatcg gtgtattaac
     2341 gctaacgggt attgcggtgg ggttggttat ccgtttcagc cagggtcatg ccggaccaga
     2401 ccccgcctgt gaaccgctga tcggcgcacc ggttccgccc tctgcgctac ctggacttat
     2461 cgtagcatta attctcggtc ttgctggcgg cgtcagcctg gggccggaac atccgatcat
     2521 gaccgtcaat atcgcccttg ccgtcgccat tggcgctcgt ctgttaccgc gcgtcaaccg
     2581 aatggagtgg actattttag cctctgccgg aaccatcggc gcgctgtttg gtacacctgt
     2641 tgcggcggcg ttgatatttt cgcaaacctt aaatggcagt agtgaagttc cgctatggga
     2701 tcgtcttttt gcgccgttaa tggcggcagc agctggtgca cttactaccg gattattttt
     2761 ccatccccat ttttcactgc ccattgcgca ttacggacag atggaaatga ccgatattct
     2821 cagcggtgca attgtcgcgg cgattgccat cgcagcaggg atggtcgccg tatggtgctt
     2881 accacggttg cacgcgataa tgaatcaaat gaaaaatccg gtgctcgtgc tgggtattgg
     2941 cggatttatt ctcggaattc tgggggttat tggtggacca gtttcgctgt ttaaagggct
     3001 ggatgagatg cagcagatgg tggcaaatca ggctttcagc accagcgatt actttttgct
     3061 ggcggtaatt aaacttgccg ccctggtcgt tgctgccgcc agtggctttc gcggtgggcg
     3121 aatcttcccg gcagtgtttg tcggcgtggc attagggttg atgctgcatg agcacgttcc
     3181 cgccgttcca gcggcaataa ccgtttcctg cgctattctc ggcatcgtgc tggtggtaac
     3241 acgcgatggc tggttaagtc tttttatggc ggcagtcgtt gtacccaata ccacattgct
     3301 accgctgctc tgtatcgtca tgcttccggc atggctgtta ttagcaggta agccgatgat
     3361 gatggtcaat cgtccgaagc aacagccacc ccacgataat gtttagcaaa aaatgctctc
     3421 ctttatgatt taagagttat ggcgatgata cggggcttta cctccccgta atattgcgtt
     3481 aacaggccgc tgacggcttc gatcgtgaag gagaataaca atgttcaggt cactgtttct
     3541 ggcggccgcc ctgatggcat ttaccccgct tgcagcaaat gcaggtgaaa tcaccctact
     3601 gccatcgatc aaattacaaa ttggcgatcg cgatcattac ggtaattact gggacggtgg
     3661 tcactggcga gatcgtgatt actggcatcg caattatgaa tggcgcaaaa accgctggtg
     3721 gcgtcatgat aatggctatc atcgtggctg ggataagcgt aaagcatatg agcgtggcta
     3781 tcgtgaaggc tggcgcgatc gtgacgatca tcgcggaaaa ggccgcggac atgggcaccg
     3841 ccattaaacg cgtcctccaa tggagcacaa tgcctgatgc gacgctagcg cgtcttatca
     3901 ggcctacaaa ccggctcttt aacgcgtagg gcggataagg cgtttacgcc gcatccgcca
     3961 atagtgccag atacgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca
     4021 gatattcagc gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct
     4081 gttcaccaga tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc
     4141 cagggcgata ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa
     4201 tcccatcaga atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg
     4261 gatatggaag cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt
     4321 tgaggacagt ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa
     4381 cagcggttgc agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa
     4441 gtggaacgcc gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat
     4501 ggcgatagcc acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa
     4561 atgctgagtg agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac
     4621 gcctgctgcc aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc
     4681 cagctgcgcc aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa
     4741 caacagtaac ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa
     4801 aatcaggaaa gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat
     4861 gagtttaaaa ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat
     4921 ttctgcctga acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc
     4981 cgccagattt ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc
     5041 catcaggttg gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg
     5101 aatattggtc gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc
     5161 cattaatgcg agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt
     5221 cgtcatcttg tgcctctaaa acatatcctt tgctatgttt catgctattc taaacgagaa
     5281 tgattatcaa attcatttaa atggattgtg gtgatttctc tgatagacca agattacgat
     5341 tacgaaaaga ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa
     5401 ctttcagctt ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct
     5461 gtcacaggac gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga
     5521 atgtgcacgg aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt
     5581 ttgtactggc acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa
     5641 aaattcgtat ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct
     5701 tcctgaactc cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac
     5761 tactcggatt tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc
     5821 tggcattctt cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta
     5881 ttctccagca cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca
     5941 aagtcaacgg tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc
     6001 agtcagaaaa ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt
     6061 acaccatggc tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga
     6121 ccatgctgga accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta
     6181 tcgtgctgtc gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt
     6241 ccaacctgca cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt
     6301 tcaaagttgc cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc
     6361 tgaacgcact gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca
     6421 tcctgggcta catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac
     6481 tgcaagtggg cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg
     6541 atctgcagaa aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc
     6601 tggtttcctt cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga
     6661 atgaagagca aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc
     6721 tggtgagtgt gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa
     6781 gccggggatc attccccggc ttttttacgc ctgttaatca gctaatggct gcgggcgacc
     6841 aatcaaatac ccttgcagat attgcacccc gagcttatgc aatagcgcct gctgctgttg
     6901 cgtctcgaca aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga
     6961 tctcacaatc atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat
     7021 tttgatgata tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggtgccaaa
     7081 gtcatcaatc gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct
     7141 ttctgcgtta gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat
     7201 gtgataacgc ttaaacagac gaataatccg tccggcaata ttcttttgca gcagcgtgag
     7261 cggcattaaa ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca
     7321 cttcaacaag gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat
     7381 aaggggcaga aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat
     7441 ctcatcataa ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc
     7501 ctgatctaac gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga
     7561 aaccatctcc tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc
     7621 cagccagctt aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc
     7681 ccaggcagca ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt
     7741 caggatgtta accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag
     7801 cagttcacta cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt
     7861 acggcaaatt gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa
     7921 ctcaagatta tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg
     7981 ttccaacgca cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca
     8041 gcggcgatta agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct
     8101 aaaggaaatc agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa
     8161 aaagttctgg ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa
     8221 cggatagcga agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta
     8281 accggcaata aagtcgtttt cataaggtgt gcacaacaga agtagcagca cgcttaacgc
     8341 tgccagccag cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct
     8401 gcgccacaat atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag
     8461 catgttgtaa atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc
     8521 atccgcatcg ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag
     8581 atacatgctg catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca
     8641 gacatatcgg ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc
     8701 gcaggcacac aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga
     8761 gagatgaaag ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg
     8821 ccagccaaag ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat
     8881 ataactggaa tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac
     8941 ggtgagcgta aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa
     9001 catgagtaca ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg
     9061 catcgcgaag caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc
     9121 aggcggcgct aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa
     9181 acccccgctt tgcagcgagg gttg
//
LOCUS       DADLTI010000154         9161 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLTI010000154 DADLTI010000000
VERSION     DADLTI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.154"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..907
                     /locus_tag="JN755_003382"
     CDS             <1..907
                     /locus_tag="JN755_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382058.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
     gene            910..1806
                     /gene="hycD"
                     /locus_tag="JN755_003383"
     CDS             910..1806
                     /gene="hycD"
                     /locus_tag="JN755_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7382059.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            1824..3509
                     /locus_tag="JN755_003384"
     CDS             1824..3509
                     /locus_tag="JN755_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7382060.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            3519..4061
                     /gene="hycF"
                     /locus_tag="JN755_003385"
     CDS             3519..4061
                     /gene="hycF"
                     /locus_tag="JN755_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7382061.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            4061..4828
                     /gene="hycG"
                     /locus_tag="JN755_003386"
     CDS             4061..4828
                     /gene="hycG"
                     /locus_tag="JN755_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7382062.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            4825..5235
                     /locus_tag="JN755_003387"
     CDS             4825..5235
                     /locus_tag="JN755_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7382063.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            5228..5698
                     /gene="hycI"
                     /locus_tag="JN755_003388"
     CDS             5228..5698
                     /gene="hycI"
                     /locus_tag="JN755_003388"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7382064.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            5759..6691
                     /locus_tag="JN755_003389"
     CDS             5759..6691
                     /locus_tag="JN755_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382065.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            6675..6986
                     /locus_tag="JN755_003390"
     CDS             6675..6986
                     /locus_tag="JN755_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7382066.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            6983..7402
                     /locus_tag="JN755_003391"
     CDS             6983..7402
                     /locus_tag="JN755_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7382067.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(7517..8941)
                     /locus_tag="JN755_003392"
     CDS             complement(7517..8941)
                     /locus_tag="JN755_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7382068.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(8950..>9161)
                     /locus_tag="JN755_003393"
     CDS             complement(8950..>9161)
                     /locus_tag="JN755_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7382069.1"
                     /translation="GLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIP
                     VEEAAAQARKHQSVQPTVAKEVSLN"
BASE COUNT         2072 a         2400 c         2521 g         2168 t
ORIGIN      
        1 aacgggtatc gcgctcgaac aaccggcgct gattgctctt ggcctggtcg gtggtctgta
       61 ccatctgctt aaccatagcc tgttcaaaag cgtactgttc ctcggggcgg ggagcgtctg
      121 gttccgtacc ggtcatcgcg atatcgaaaa actcggtggt attggcaaga aaatgccggt
      181 tatctccatc gccatgttag tcgggttgat ggcaatggct gcgctgccgc cactgaacgg
      241 gtttgccggg gaatgggtta tctatcaatc cttcttcaaa ctgagcaata gcggcgcgtt
      301 tgttgcccgt ctgctggggc cgctgctcgc tgtgggtctg gcaattaccg gtgcgctggc
      361 ggtgatgtgt atggcgaaag tctatggcgt cacgttcctc ggcgcgccgc gtaccaaaga
      421 agccgaaaac gccacctgtg cgccgctcct gatgagcgta agcgtagtgg cactggcgat
      481 ttgctgcgta attggcggtg ttgctgcgcc gtggctgctg ccgatgctct ctgctgctgt
      541 acctctgcct ctggagcctg ctaacaccac cgtttctcaa ccaatgatca cattgctgct
      601 gattgcctgc ccgctgctgc cattcatcat tatggcgatt tgcaaaggcg accgcctgcc
      661 gtcacgttcc cgcggtgcgg cctgggtgtg cggctacgac catgaaaaat caatggtgat
      721 taccgctcac ggttttgcca tgccggttaa acaggcgttt gcgccagtgc tgaaactgcg
      781 caaatggctg aatccggtgt cgctggtgcc gggctggcag tgcgaaggta gtgcgttgct
      841 gttccgccgg atggcgctgg ttgaactggc ggtactggtg gtgattattg tttcacgagg
      901 agcctgagaa tgagtgtttt atatccgtta attcaggcgc tggtgttatt tgccgttgcg
      961 ccgctgctct ccggtattac ccgcgtggcg cgcgcccgtc tgcataaccg tcgcgggccg
     1021 ggcgtgttgc aggagtatcg cgacattatc aaactgctgg gccgccagag cgttggcccg
     1081 gatgcctctg gctggatgtt ccgcctgacg ccgtatgtga tggtgggcgt catgctgact
     1141 atcggttctc cgctgccgca actgggcgat ttgatcacct tactgtatct cttcgccatc
     1201 gcgcgtttct tctttgccat ttccggtctg gataccggta gcccgtttac cgctatcggt
     1261 gcgagccgtg aagcgatgct tggcgtgctg gtcgaaccga tgttgctgct tggtctgtgg
     1321 gttgccgcac aggttgccgg ttccaccaac atcagcaaca ttaccgacac cgtttatcac
     1381 tggccgctga gccaaagcat cccgctggtg ctggcgctct gcgcctgtgc gttcgccact
     1441 tttatcgaaa tgggcaaact gccgttcgac ctggcggaag ccgagcagga gttgcaggaa
     1501 ggtccgctct ccgaatacag cggtagcggc tttggcgtca tgaaatgggg tatcagcctg
     1561 aaacagctgg tggtgttgca gatgttcgtc ggggtgttta ttccgtgggg acaaatggaa
     1621 accttcaccg tcggtggact gctgctggcg ctggtgattg ccatcgtcaa actggtggtc
     1681 ggcgtcctgg ttatcgcgct gttcgaaaac agtatggccc gtctgcgtct tgatattact
     1741 ccgcgcatta cctgggctgg gtttggcttt gcatttttag cgttcgtctc cttgctggcg
     1801 gcgtgattaa agagagtttg agcatgtctg aagaaaaatt aggtcaacat tatctcgccg
     1861 cgctgaatga ggcatttcca ggcgtcgtgc tggaccacgc ctggcagacc aaagatcagc
     1921 tgactgtcac cgtaaaggtg aactatctgc cggaagtggt ggagtttctc tactacaagc
     1981 agggtggctg gctgtcggtg ctgtttggta acgacgaacg caaactgaat ggtcattacg
     2041 ccgtttatta cgtgctgtcg atggagaagg gcaccaagtg ttgggtaacg gttcgcgtcg
     2101 aagttgacgc caacaaaccg gagtatccgt ccgtgacgcc gcgcgttccg gcggcggtgt
     2161 ggggcgagcg cgaagtgcgc gatatgtacg gtttgattcc ggttggtctg ccggatgact
     2221 ggccggatga actttatccg ctgcgtaaag acagcatgga ttatcgtcag cgtccggcac
     2281 cgactaccga tgctgaaacc tacgagttca tcaacgaact gggcgacaag aaaaacaacg
     2341 tcgtgccgat tggtccgctg cacgtcactt ctgacgaacc gggtcacttc cgtctgttcg
     2401 tcgatggcga aaacattatc gacgccgact accgtctgtt ctacgtccat cgcggtatgg
     2461 aaaaactggc ggaaacccgc atgggttata acgaagtgac cttcctctct gaccgtgtgt
     2521 gcgggatctg cggctttgcc cacagcaccg cctacaccac gtcggtggaa aacgcgatgg
     2581 gtattcaggt gccagaacgc gcgcagatga tccgcgccat tctgctggag gtggaacgcc
     2641 tgcactcgca tctgctcaac ctcggcctcg cctgtcactt taccgccttc gactccggct
     2701 ttatgcagtt cttccgcgtg cgtgaaacct ccatgaaaat ggcagagatc cttaccggtg
     2761 cgcgtaaaac ctacggcctg aacctgatcg gcgggattcg tcacgatctg ctgaaagatg
     2821 acatgatcca gacccgccag ctggcgcaac aaatgcgtcg tgaagtgcag gagctggtgg
     2881 atgtgctgct gagtacgccg aacatggaac aacgcaccgt cggcattggt cgtctggacc
     2941 cggaaatcgc ccgcgatttc agtaacgttg gcccgatggt tcgcgccagc ggtctcgccc
     3001 gcgatactcg cgccgatcac ccgtttgtcg gttatggcct gctgccaatg gaagtccaca
     3061 gcgagcaggg ctgcgacgtt atttcccgtc tgaaagtgcg aatcaacgag gtctataccg
     3121 cgctgaatat gatcgactac ggtctggata acctgccggg cggtccgctg atggtggaag
     3181 gctttaccta cattccgcac cgtttcgcgc tgggctttgc cgaagcgccg cgcggcgacg
     3241 atatccactg gagcatgacc ggcgacaacc agaagctgta ccgctggcgc tgccgtgccg
     3301 cgacctacgc gaactggccg accctgcgct acatgctgcg tggcaatacc gtttccgatg
     3361 cgccgctgat tatcggtagc ctggaccctt gctactcctg taccgaccgc atgaccgtag
     3421 tcgatgtgcg taagaagaag agcaaagtgg tgccgtacaa agaactcgag cgttacagca
     3481 ttgagcgtaa aaactcgccg ctgaaataag gaatcgccat gtttaccttt atcaaaaaag
     3541 tcatcaaaac cggcacggcg acctcgtctt atccgctgga gccgattgcg gttgataaaa
     3601 acttccgtgg taagccagag cagaacccgc agcagtgcat cggctgcgcg gcttgcgtca
     3661 atgcctgccc gtcaaacgcc ttaacggttg aaactgacct cgccacagga gagctggcgt
     3721 gggagtttaa tctcgggcgc tgcatcttct gtggacgctg cgaagaagtt tgcccgacgg
     3781 cggcgatcaa actgtcgcaa gagtacgaac tggcggtgtg gaagaaagaa aacttcctgc
     3841 aacagtcccg cttcgcgctg tgcaactgcc gcgtctgcaa tcgtcctttc gccgtacaga
     3901 aagagatcga ctacgccatt gcgctgctta agcacaatgg tgacagccgc gcggaaaacc
     3961 accgcgaaag ctttgagact tgcccggaat gtaagcgcca gaaatgcctg gtgccgtccg
     4021 accgtattga actgactcgc catatgaaag aggccatctg atgagcaatt tattaggccc
     4081 ccgtgacgcc aacggcattc cggtcccgat gacggtggat gaatccatcg ccagcatgaa
     4141 ggcgtcgtta ctgaaaaaaa tcaaacgttc tgcctatgtt taccgcgtgg actgcggcgg
     4201 ctgcaacggc tgcgaaatcg aaattttcgc cacgctttct ccgctgttcg atgcagaacg
     4261 cttcggcatt aaagtcgttc cttcaccgcg tcatgcggat attttactgt ttaccggcgc
     4321 ggtcacccgt gcaatgcgat cccctgcgct gcgtgcgtgg cagtccgcgc cggacccgaa
     4381 aatctgtatc tcatacggtg cctgcggtaa cagcggcggg atcttacacg atctctactg
     4441 cgtgtggggc ggtacggata aaattgtccc tgtggatgtt tatatccctg gctgcccgcc
     4501 aacgcctgcc gccacgctgt acggctttgc aatggcgctc ggcctgctgg agcagaaaat
     4561 tcacgcccgt gggccgggtg aactggatga acaaccggcg gagatcctgc atggcgatat
     4621 ggtgcagccg ctgcgcgtga aagtggatcg cgaagcacgt cgcctggcgg gttatcgtta
     4681 cggtcgtcag attgccgatg attaccttac acagttaggg cagggcgaag aacaggttgc
     4741 acgctggctg gaagcggaaa acgatccgcg tctgaacgag attgtcagcc atctgaatca
     4801 tgttgttgaa gaggcgcgta tccgatgagt gaaaaggtgg tgttcagtca actgagccgt
     4861 aaatttattg atgagaacga tgccacgccc gccgaggcgc agcaggtggt ctattacagc
     4921 ctggcgattg gtcaccacct tggggttatc gattgcctgg aagcggcgct cacctgccca
     4981 tgggatgaat atctggcatg gattgccact ctggaggcgg gcagcgaagc ccgccacaaa
     5041 atggaaggcg taccgaaata tggcgagatc gtcatcgaca ttaaccatgt gccgatgctg
     5101 gccaacgcat tcgataaagc ccgggcagcg caaacttcgc agcagcagga atggagtaca
     5161 atgctgttaa gtatgctgca tgatattcat caggaaaacg ccatctattt gatggtgagg
     5221 agactgcgtg actgacgttt tactctgtgt tggcaatagc atgatgggcg atgatggcgc
     5281 tggcccgctg ctggcggaaa agtgcgccgc cgcgccgaaa ggtaactggg tggtgattga
     5341 cggcggtagt gcaccggaaa acgacatcgt cgccatccgt gaactgcgcc cgacacgact
     5401 gctgattgtc gacgccacgg atatggggct aaaccccggc gaaatccgca tcatcgaccc
     5461 ggatgatatc gccgagatgt ttatgatgac tacccataac atgccgttga attaccttat
     5521 cgaccagtta aaagaagaca ttggtgaagt gattttcctc ggcattcagc cggatatcgt
     5581 cggcttttac tacccgatga cccagccgat taaagatgcg gtagaaaccg tttatcaacg
     5641 actggaaggc tgggaaggaa atggtggatt tgcgcagtta gcggtggaag aagaataatt
     5701 ctccatcagg gaattcaggg aattcaggga attcagggaa ttgaacatca gaacagggat
     5761 gtgcgctttt ttattgagct tagtgctgcc tgcacaggcc accagcttta ccgaatatct
     5821 gccaatgagt gacagcgaat acgcgcaaaa gcgggcgctg aaacctctcc tgaccatgcc
     5881 ttatgatgcc gagcaaacct ggcattttag gaaagtgggc gttgctggcg tgacgcttga
     5941 gaagatgcca aatgaagaag attactggca attaaccgct aaagaccgcg ccggtaaatc
     6001 ctggactgtg cctgtgggta tgctggcaaa catagcgggc aaagggcaac tctatcgcgc
     6061 cgatcttgat cgtaacggca ttcaggatct ggttatctgg ttaccaagta ccggtaatgg
     6121 tctggctcct tatgcacatt taattcttat gacgttcacg cgcgaaggcc gtccctgtgt
     6181 ctttgaaccc aggggattct acaccgcaag caaaactggc gtagatgatt tgctggattt
     6241 gcaaggcaat ggtcacacgc aattgctgga tatgcagttt aattctggtt actggataac
     6301 cagtctgtat caggtaaaag acgccaaatg gcagcgtgtt cacggctggt ttgacaagtt
     6361 aagctatccc gcgctcacgc gttttactta tacccccaac cgtaaactcg tcctcaaacc
     6421 catcgccggg cgcgacccgc aaacggaaga tctggcgcag acgcagcgtt gcttgataaa
     6481 gggggatgtg cttgaggggg gaaccagaac tgactttttg cagagtggct atgaggattt
     6541 tgggggtaat tatatcgcgc acaagtgttt acatattgtg aacttggatt tgatgagcta
     6601 tagtttccga acacataagg atagaactcg cagatgtggc tgtttactta ctataaggaa
     6661 gttaaaactt aaaaatgcac atcatttcta aagcgccatt tgaagagtct gcgcgtaaat
     6721 atcctaacga cgctctggct cttcaggcat tgtatcgggt tattaaggaa acggattttt
     6781 ctacgccaga agagatgcga acggcgtttc ccaacctcga caattttaaa tatcgcaaca
     6841 aatggtacgt tctggatgta ggtgggaaca acctaggggt gattgcctac ataaactttg
     6901 ttaataagcg gttctttgtg aagcacataa ctaatcacgc cgagtatgac aaactgacac
     6961 gttactacag agagaacaaa gaatgactgc taatgctgca cacgctgtta aagcaacaag
     7021 agaactggtc aatgcagtcc cctttctggg aggtagtgac tcagaagatg attatcgtga
     7081 agcactggaa ctggtcgaat atttgattga agaagatgac accaatccac ttatcgactt
     7141 tctggcgagc cgcattgcag aatatgaaaa caacaaccaa gagtttgcag agttcgacaa
     7201 agccgttgcg gcaatgcctg ttggcgtggc gttacttcgc acgctgattg atcaacataa
     7261 tctgacctat gcggatttga agaacgaaat cggctctaaa tccctggtca gtcagatttt
     7321 gtctggacaa cgatccctga cgatatccca cattaaggca ctttcagccc gatttggcgt
     7381 taagccggaa tggtttcttt gatttttgac taatgccaaa tgctaaattg cccgatgcgc
     7441 tgcgcttatc gggcctacat gtcgcacgaa ctatgtaggc cggataaggc gttcacgccg
     7501 catccggcac tgttacttac tctaaatctt ccccattact ggcaatcact tttttatacc
     7561 accagaacga tttcttacgt gtgcgcgtca gcgtgccgtt gcctgcgtcg tcacggtcta
     7621 cgtagacaaa accgtagcgt ttgctcattt cacccgtaga ggcggaaact aaatcaatac
     7681 agccccatgt ggtgtagccc atcagcggaa tgccatctgc aatcgcttcg cctattgcgc
     7741 ggatatgttc gcgtaaatag ctgatgcgat agtcatcgtt aatctcgcca ttggcagcaa
     7801 gttcatcttt tgcgccgagg ccgttttcca ccagaaacag cggcttctga taacgatcgt
     7861 acatcatatt catggtgata cgcagaccga gtggatcaat tccccagccc cagtcgctca
     7921 cttgcagata cgggttacgc agcgatttca ccacgttcgc cgcactgctg ttgttggcgt
     7981 tcatctccgc cgaggcgcag cgcgaggcgt aatagctgaa agagacaaaa tcgacggtgt
     8041 ttttcaggat ctcatcatcg cccagcgctt tatctatggt tacccctttt tcgcgaaata
     8101 cgcgggcaga gtaagccgga tacgcgccgc gcgcctgcac atcgataaaa aacaggtttt
     8161 cccgatcttt ctccagcgcc gcccagacat cttccggttt gcagctgtaa gggtagaagt
     8221 taccgcccgc cagcatacag cccacctgat tttgcgggtt aacctcatgg gcgattttgg
     8281 tggctagcgc actggcaacc agctggtgat gcgcggcctg atatttcacc tgatcctgat
     8341 tttcaccttc ttcaaacacc agacccgcgc cggagaacgg gctatgcaac ataatgttga
     8401 tttcattgaa ggttagccag tatttcacca gaccatcaaa tgcttcaaag caggttctgg
     8461 cgtagcggct gaaaaactcc accagcttgc ggttacgcca ggagccatat tcggtgacca
     8521 gatgcatcgg cacatcgaag tggcacaacg tgaccagcgg ttcgataccg tactttttac
     8581 actcttcaaa gacaggacga taaaaagcaa tgccctgctg attgggcgtg atttcatcgt
     8641 cctgcggaaa gagacggctc caggcaattg aggtacggaa aaccttgaat cccatctctg
     8701 ccatcagggc gatatcttct ttataacgat gataaaaatc cgtcgcctca tggctgggat
     8761 aaaactcgtc atcgcgcaac tgaaaacgtt tttccagccc cagtttcacc gccattcgat
     8821 gctcgccgtg tgggatcata tcgacggtgg tcagcccttt tccgccttca cggaacgcac
     8881 cttcagactg gttggcggca agcgcgccgc cccataaaaa accttctgga aatactgaca
     8941 ttttcatcct caattaagac ttacttcttt ggcgacggtc ggttgtacgc tctgatgctt
     9001 tcgcgcctgg gcagccgctt cctcaacagg aatatcctca aagccgagca acagtgtgag
     9061 gataaacgac agcaccaccg ccagcgccat gaccgcgaac acccagacga tgctcattgg
     9121 attcgccgga tcgaagaatt gcacgctggt aaatagcccc g
//
LOCUS       DADLTI010000155         9069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLTI010000155 DADLTI010000000
VERSION     DADLTI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.155"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JN755_003394"
     CDS             complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JN755_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7382070.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JN755_003395"
     CDS             complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JN755_003395"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7382071.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JN755_003396"
     CDS             complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JN755_003396"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7382072.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3790..4299)
                     /gene="def"
                     /locus_tag="JN755_003397"
     CDS             complement(3790..4299)
                     /gene="def"
                     /locus_tag="JN755_003397"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7382073.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4464..5588
                     /gene="dprA"
                     /locus_tag="JN755_003398"
     CDS             4464..5588
                     /gene="dprA"
                     /locus_tag="JN755_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7382074.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5560..6033
                     /locus_tag="JN755_003399"
     CDS             5560..6033
                     /locus_tag="JN755_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7382075.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6062..6604
                     /locus_tag="JN755_003400"
     CDS             6062..6604
                     /locus_tag="JN755_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7382076.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6609..7181
                     /gene="tsaC"
                     /locus_tag="JN755_003401"
     CDS             6609..7181
                     /gene="tsaC"
                     /locus_tag="JN755_003401"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7382077.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7186..8004
                     /gene="aroE"
                     /locus_tag="JN755_003402"
     CDS             7186..8004
                     /gene="aroE"
                     /locus_tag="JN755_003402"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7382078.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8001..8258
                     /locus_tag="JN755_003403"
     CDS             8001..8258
                     /locus_tag="JN755_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7382079.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8234..8788)
                     /locus_tag="JN755_003404"
     CDS             complement(8234..8788)
                     /locus_tag="JN755_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7382080.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2278 a         2199 c         2261 g         2331 t
ORIGIN      
        1 aacaaatgtt aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag
       61 ggaaataata aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga
      121 agagtctttc gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc
      181 cttgctcaat gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc
      241 caataatcgt tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg
      301 tgctttcatc accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg
      361 aaacaccaac aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt
      421 gtggtgaaat tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct
      481 ggatcaatac catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat
      541 tggcctcgtc atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg
      601 ccagcagttc ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg
      661 ccagttcggc agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca
      721 gacgacgcgc cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg
      781 gtttttccag tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa
      841 agaacacttc atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt
      901 ggcggaaaat tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa
      961 gtgcattacc aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac
     1021 cctcagcgaa gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat
     1081 cgataaccaa ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata
     1141 gcttatcggc atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt
     1201 tgaaaagtga gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag
     1261 caaccagcat atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat
     1321 gcccctgcac gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg
     1381 tatcgacaac agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa
     1441 cctggccggc acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt
     1501 tgattagctt agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac
     1561 ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg
     1621 ctttaatctg caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc
     1681 caccggattt taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt
     1741 ccgggatatc gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg
     1801 ttgctgaaca aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg
     1861 aagggtaacg gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt
     1921 cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg
     1981 ccacctcaag gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt
     2041 caccgttttg tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa
     2101 cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag
     2161 catcagggta atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc
     2221 agctgtcgcg ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat
     2281 tggcttcggc gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc
     2341 aggaaggatg cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc
     2401 gacgctggaa ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg
     2461 cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca
     2521 gttgatacaa accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac
     2581 gggccattaa cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc
     2641 acaactcttg aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa
     2701 tgttgcttaa tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac
     2761 gtaaattacg ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag
     2821 tggactatca gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt
     2881 cttgcgcgct catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat
     2941 caccagtcgc aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt
     3001 tggttgccgt atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca
     3061 gccagctcat tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa
     3121 gtgaccagtc aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt
     3181 cgtcctgaac ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc
     3241 cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa
     3301 tcgggcagga gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa
     3361 tggttacacc agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc
     3421 gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca
     3481 cagctttagg cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg
     3541 cgaccagttg ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt
     3601 cttcctcagc cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc
     3661 ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat
     3721 gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag
     3781 ttcttatcct taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg
     3841 ttgttgtttc agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc
     3901 atgctgaata cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg
     3961 gtcaagggcg cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat
     4021 cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat
     4081 taacaccagc cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat
     4141 atcaacctgg gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc
     4201 atcgacgata cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag
     4261 ccgctcgtcc ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca
     4321 ggagttagaa agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac
     4381 ttgattgaca gcatcacttg attgacagca tcactgacca atcgcaaaga ttgctaaggc
     4441 ttgcttatga cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagca
     4501 tcagcagctt gtacggcgat gatatggtcc gtatcgctca ctggctggca aaacagtcgc
     4561 atattgatgc ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc
     4621 tttcatttcc acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc
     4681 atttaatccc tgcggacagc gaattttatc ctcctcaact tcaggcgacg acagattacc
     4741 ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag
     4801 tggggagtcg ggcgcattca tggtatggcg agcgatgggg gcgattattt tgcgaaactc
     4861 tggcgacgtg tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggtgc
     4921 ataaagcggc cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata
     4981 tcattcatcc gcgtcgccat gcccgactgg ctgccagtct gtttgaacag ggtggcgctc
     5041 tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc
     5101 gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt
     5161 cgctggtgac agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc
     5221 caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg
     5281 tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg
     5341 cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca tttcctgagc
     5401 tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc
     5461 aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag
     5521 ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat
     5581 ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga caaacttgaa
     5641 caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg
     5701 gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct
     5761 ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg
     5821 tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata
     5881 gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc
     5941 ctgatggtgt tgttcaacat cccgggctgc gaaaatgcgt accagcaaat ggaagaatta
     6001 ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt
     6061 tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc
     6121 tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc
     6181 gtgtgactac gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga
     6241 ggggcaggtt tgccccgtat gtggcgcaaa tctggtatta cgccagggac gctttggtat
     6301 gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga
     6361 cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg
     6421 ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt
     6481 caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac
     6541 cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga
     6601 ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata gatgtcctca
     6661 atgaaaaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgtcggg tgcgatcctg
     6721 atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt gataaggggc
     6781 tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac accatgttga
     6841 ctgacgtgca gcgtgaaacc attttttcct gctggccagg tcctgtcacc tttgtctttc
     6901 ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag
     6961 tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta
     7021 ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat
     7081 ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa
     7141 tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct
     7201 gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag
     7261 caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcatcaac
     7321 acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt gccttttaaa
     7381 gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgcagtt
     7441 aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc
     7501 ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc
     7561 ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg
     7621 acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact
     7681 ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt
     7741 aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat
     7801 ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt tctggcatgg
     7861 tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt ggcacaggcg
     7921 gctcatgcct ttcttctctg gcacggtgtt ctgcctgacg tagaaccagt tataaagcaa
     7981 ttgcaggagg aattgtccgc gtgaatcagg ccatccagtt tccggacagg gaagagtggg
     8041 acgagaataa aaaatgtgta tgttttcccg ctctcgtgaa tggtatgcaa ctgacatgcg
     8101 cgatctctgg cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag
     8161 cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac
     8221 aaagtgaaga tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt
     8281 tcacgtaatt attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga
     8341 tctgtttgac ggggctaccg agatacagat atccgctctc cagccgttta ttttgtggga
     8401 ccagactacc cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg
     8461 agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac
     8521 caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat
     8581 gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat
     8641 gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt
     8701 caccaatcac gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat
     8761 cgcggtatgg gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga
     8821 ctttgggggc attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt
     8881 gtgcgattgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac
     8941 ttgtgcaaaa tattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact
     9001 tcacaggatg gtcggaacaa cgaagagaaa aaatcctgaa attcagggtt gactctgaaa
     9061 gaggaaagc
//
LOCUS       DADLTI010000156         9068 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLTI010000156 DADLTI010000000
VERSION     DADLTI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.156"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            451..813
                     /locus_tag="JN755_003405"
     CDS             451..813
                     /locus_tag="JN755_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7382081.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            810..1727
                     /locus_tag="JN755_003406"
     CDS             810..1727
                     /locus_tag="JN755_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7382082.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1740..3005
                     /locus_tag="JN755_003407"
     CDS             1740..3005
                     /locus_tag="JN755_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY7382083.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3072..3695)
                     /locus_tag="JN755_003408"
     CDS             complement(3072..3695)
                     /locus_tag="JN755_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382084.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4437..4784)
                     /locus_tag="JN755_003409"
                     /pseudo
     CDS             complement(4437..4784)
                     /locus_tag="JN755_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4906..5166
                     /locus_tag="JN755_003410"
     CDS             4906..5166
                     /locus_tag="JN755_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY7382085.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5184..7919)
                     /locus_tag="JN755_003411"
                     /pseudo
     CDS             complement(5184..7919)
                     /locus_tag="JN755_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7920..>9068)
                     /locus_tag="JN755_003412"
     CDS             complement(7920..>9068)
                     /locus_tag="JN755_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7382086.1"
                     /translation="GVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQIES
                     VRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVAQMY
                     QQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAITMRG
                     QDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQLQQK
                     QQALNDGYKDGINTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDT
                     FKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKR
                     NEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         2365 a         2087 c         1935 g         2681 t
ORIGIN      
        1 atcaccttac cgaacacgca cggcaaatag actcgatcct gaacccatcg gtcccaaatt
       61 tgtcccagtc aaaaaataag gaaagtacta atgatgtgta acttattgat ttaaatggtg
      121 ccgataatag gagtcgaacc tacgaccttc gcattacgaa tctgtagcac caatcataac
      181 tatctgtttt agcaagcatt aaccgcattc actaagatat agttgatggc acaaacagaa
      241 agttgatgaa tgatgttgcc atatatatgt cacaaatacg gcacaacgat cttcaaacat
      301 atagccacac attcacagaa gagcacaaag ccttgcaatc cagtgcaaag atttgtgtgc
      361 cagagttttt ctcatcaact accgcaagta tcgatcgatt gagacttgga tgatagactt
      421 catgcctttg attattagct gatagaagaa atgttaaagc tatttgcaaa gtacacatct
      481 ataggcgtgc tgaacacact tatacactgg gtggtttttg gtgtatgtat ctatgccgcg
      541 cataccaatc aggctatggc aaacttcgca ggtttcgttg tggctgtgag ttttagcttc
      601 tttgcgaatg caaaattcac attcaaagca tcaactacaa cgatgcgcta catgttatat
      661 gtcgggttca tgggaacact gagtgctact gttggatggg ttgctgatag atgttcactt
      721 cctccaattg tcactcttgt taccttctcc gccatcagct tggtgtgcgg tttcgtctat
      781 tcaaagttca ttgtctttag ggatgcgaaa tgaagatatc tcttgtcgtt cctgtcttca
      841 atgaagaaga agcgatacca attttttata aaacggtacg tgaattcgaa gagttgaagc
      901 catatgaagt agaaattgtt ttcataaatg acggaagcaa agacgctacg gagtcaatca
      961 ttaacgctct ggctgtttct gatccgctag ttgttccgct gtcatttaca cgcaactttg
     1021 gtaaagaacc agcattgttt gcagggttag accatacaac cggcgatgcc gtgattccaa
     1081 ttgatgttga cctgcaagac ccgattgagg ttattcctca tcttattgaa aagtggcagg
     1141 caggtgctga catggtgctt gctaaacgtt cagaccgctc aactgatgga cgactgaaac
     1201 gtaagacagc tgagtggttc tataaattac acaacaaaat aagcacccca aaaatagagg
     1261 aaaatgtcgg agattttcga ctcatgtctc gtgaggttgt ggagaacatt aaactattgc
     1321 ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt tggtggtcag acagatgtcg
     1381 ttgaatatgt acgcacagag cgtgttgctg gcatctcaaa atttaatggc tggaaattat
     1441 ggaatctggc actggaaggt atcacaagtt tttcaacctt tcctcttcgc gtatggactt
     1501 atataggctt gtttgttgca agcatttcat ttttatatgg tgcatggatg attatagaca
     1561 ccattgtctt tggtaaccca gtacgcgggt atccctccat gcttgtatca atacttttcc
     1621 tgggtggagt gcaactgatc gggattggtg ttctcggtga atatataggt agaatttatt
     1681 tagaaacaaa atcaagacca cggtacttaa ttaaaagcag aaaataacag gatgatctta
     1741 tgaagatatt cataatgcgc aattggcaca agatatcaat ttttatactt gcatttactt
     1801 tgatttggct taggcgcatt gacattttga caaatgccca gttttgggct gaggatgcag
     1861 ttttttggta taaagatgca tacgaacaag gttttttcag ttcactcata accccaagaa
     1921 atggttattt tcaaacagta tcaacactca ttgttggtgc gacgacattt attaatccaa
     1981 tttacgcacc attattatca aacttttttg gaataataat tagagctata attatatggt
     2041 tcttatttac cgatagattt aaattcctaa gcaccacatc aaaaatcttc atctctgtat
     2101 atttaatttg catgcccggg cttgatgaag tccaagccaa tataacaaat gctcactggt
     2161 atttatcatt gtatgttgca atgataataa tatctgaaga atccaagtca aaattatgga
     2221 aagcacatga tttatttttt atagtattat ctggattgag cggtccattt attatattca
     2281 taattgcatc atctgttttt aaatatttac acttatcaaa aggagtaata agcataagag
     2341 gtttatactt attttatact cgactgccat atttggcaat gatcttgtgt gggctgatac
     2401 aggcaacatc aataattctt acatttaatg gaacaagatc acatgctcca ttaggattca
     2461 gttttgatgt tatgtcatct ataatatcat ctaatgtatt tcttttttca tttgtccctt
     2521 ggaatatcgc taatgctgga tgggataatc accttttatc gtacacacta tcaactttag
     2581 taattacatt atgtgttttc atttatatta aaggaaattg gcaaatgaaa gtgtttgcca
     2641 cccttccaat acttattgtc gcattttcta tggcaaaacc tcaactggca gattctgttc
     2701 ctcaactgcc aacacttgca accggaaacg ggtcaaggta ttttgtaaat attcatattg
     2761 caattttttc attaatatgc gtttgtttgt ttcaatgcat aaagaataaa accttaaaaa
     2821 tattctttaa aatctatgtt tcagtcatcc ttattgcaat gataaaatta aactttttta
     2881 taacccctct tcctgacatg aactggtctc aaggagcaga attaataaat aaagcaaaac
     2941 atggagaagc cgtctcattt acagtgctcc ctccatggtt aactcttgat ttaataaaaa
     3001 aataaaactt cacattaata aataacaatg aatccgcctc cctacgtatt cccgtaggga
     3061 ggctgtcgtg tttatagtag cctggtaatg ctaaatgcaa acgaactgga gattcctgct
     3121 ccgctaagcg tattgcgcag ggtcacccta aatgtatcta caccaatata agtaacatca
     3181 caactaactg aatcaggcgc agatataggt gttatctgaa ccacataacc attagtagct
     3241 gctgcgagcg gatgagaaaa cgttacatca tacacaccag tagcatgcgc gttaacggat
     3301 gaaacacccg gcgcaatgtc tacactacca ttggcgttaa ttctccctga gcgcgtaact
     3361 gactctgcag aaaccgatgt ggccttactg gttatttcca taaaggtgca accaatatcg
     3421 atcaattcgc aattgttccc tgttatccag tatggtcgat ctgcacttgg ctgataatcc
     3481 cctgcgctct ggaacgttgt gccattgagt aagactataa cttttccccc acctgagctt
     3541 gttacttgta tattcgtgtg cgtgtagcgt tcactgctca cgcgatgaaa atttccaccg
     3601 tttataacta ccgttacagg tcgcgtccca gtattatcta tagcaaggtc tgcccccccg
     3661 gcattagcct cgaagtatgg gttgtttaac accaaagcac acgaaccgtt aaggccattt
     3721 atggctaatt gcgcaccacc tgtaccggaa tccccctgag taccatttcc ttcacaagtc
     3781 aggctgttga tagtagttcc tgccccccat ctatcaccaa gtatacccca tcgtgtattc
     3841 tggcttgcag ttaatcggtt aactgtaatg gcattaagct ctgacccttc actggagtta
     3901 agtgcgcgcc agccaatacg gttaccctgt aagtacacat tatctaactc tgacgttaac
     3961 accgagtctg tacgcaaccc ttcgccggta gtgtgcccat aagacacaat gttctctaat
     4021 ttagtgtatg ctttattggt tacgagaaga ccgtaagacg atcccgaacc tttaagtgta
     4081 atgtccccca gataatcata ggcgtgtaca cgttgggttg ccccgactgt atcaccgagc
     4141 atctttaagt tgaaatccga ggcatggcag ttaaatatcg tttctgccat attttctccg
     4201 gaataatgag cacggactcc tgtcgaacca ataaatttag cgtcatagtt gccgtaatca
     4261 agaacgagtg gtgacgcgat atcgtacacc ccttgcttta tttttaatgt tccaccgaaa
     4321 ggccacgatg taagcgggtc ttgatatatt tcatttttta ttgcaaatag ctcctcttgg
     4381 agatttttgc cgctattagt gccaatgtga gcagctcctt catctgattg aagtaactgc
     4441 tttaactgat caggatcgta cttcagcaca ttctgaaaat agaactgctg cgaaccatac
     4501 gcatcatata cagccattga atggccttgc tcagttacaa atttggcaat ctgcccgttg
     4561 tacacaggat atccggcagc attgataaca attggctgag aaacagggac gtgagatccg
     4621 tcttcattct ctacataaac ctgaatctgg ttttcaggat ttaccgggtc agtgtcaatt
     4681 tttccgatat aaattttgcc attggctacg gctttaaaag aacgcgccat agtgaagagt
     4741 tgcgaaggca tgctgaccac aacatttgcg gcgatatctg acatttcatt gctccagacg
     4801 aatgatatga tgcaatcatg atgtgattgc ataccgaaat ggcactattg aatatttatc
     4861 cagtaggtta cgatgccatt ccacccaact ggtgaggcat caaggatgta cagcaaatac
     4921 gacgaggcac agtttcactt gagacttccg catgaactcc acgcgaaaat taaacagtgt
     4981 gcgaagatga ataacagttc gctgaactca gagataattg cagcgattga agaatcattg
     5041 gctaaacaaa gctctgcatc agtttacatt gacgatgcag agcgtatggc agaacaacaa
     5101 tctgatatgg ttaagaaaat tgtctttgat acgctcaagg agctatataa aaaagacagc
     5161 aactaaccat ccgtggcttt ccgttacttc tggttatcat cactgttcat ccagtacatg
     5221 atgcctagcc ttgatatggt tttcttatct gattctggaa gtgagttgta aaacgagcgc
     5281 caagcagaag agcgtcttac agttgtatca acgatgcttt ctggtgcttg ttttactgcc
     5341 agccttgctg ctttctggaa ttctggtgag ctaattagct tttctgcaga ttcaattgat
     5401 tcagcgccgc ccatcgccct gattttgctg gcgacttttg ccccaatttc tgacccagca
     5461 acagcaccta ctgcactgaa cggtcctgac acagttgaac caaccgcagt gccaatgcgt
     5521 tgggcatggt tagcaacaaa ttcatttgcc gcagtgacgc gattaaaccg tttgacgaac
     5581 tcgtttagtc ttcctgtagt tatctcgtaa gattttgcat ctttgatagc ctttgcgacc
     5641 ttatatacat cgttcagacc tgacatagtc tcttttggta aatgtctggc aagattgcgc
     5701 atctgcccgt ttgctaacat gttttgatac cagtcagcaa accctgcagg attaaaatca
     5761 gcgccacgtt ttccgttcga aagcatatcc cgaagacctg ttcccagtag ctcggttctt
     5821 agcttcctgg acggcgtgtt ttgcatcaat tcacgaaatc ctttggcatc gcctttcgac
     5881 attgcctgta gtgctgtagt tgctttgcga gaaacatcac cgttcagcgt tctgccagtt
     5941 agattaatca tttgctcttc catgcttttt cgcatctgaa caagcctctg agcaacttca
     6001 aaatcacgta atgcatttgt ctctgacagc gccgcctttt gatcatcggc gagcgaaccg
     6061 taaagcctcg ataaagcagc gcgatcagca tctttatatg gtccagaatt cttatgaagt
     6121 gcatcaccaa caagtcgtcg ttgcttattg agatttgcat aagttaatgc gccgttcttg
     6181 cctggattaa ctgcccgaaa gactctcttt tcaatagggt caaggttttt catcccgcca
     6241 agatcttctg ccacctgttt gagcattgac cttgttgatg gcgcatcaat ttttgcacct
     6301 gcaggcatcg ccttttcaac attgcgataa agctggtcgc ttcgtttcat cagcgcagac
     6361 attcttccat tgaccgtatc aataaacttg tcgctcatac caagagcatc ttttgcaccg
     6421 gaaacgcgat caattatctc acccgcgccc tgtgctagct gcctgattgc ttcattttcc
     6481 tgcacctgta gagcagaacc cgcacgggac ttgattgctt gctcaactgc cttgtattgc
     6541 tcgttcccgg aaaagtgtga agggagtaat gaatcaacat taagcctttc agcagactcc
     6601 agaacttcag cctgaggatt gatatctagt tcatcaagcg atgaagcgag atttggcctt
     6661 ttttgtgctg ctactgtgcg aacgacttcc tctgggttct tcgtcgcagg cgtcattggt
     6721 acaccttgtg ctactcttgc ctctgcggca tgaaccgcag gatttgttgc tgcagcggca
     6781 tcaccggaga atggtgatgt ttctgttggc gcagtggtcg ctctctgtgc agatgtttta
     6841 ttgccactaa ttatatttga tgcaccacga acaccacggg taacaccatt tatcaggcca
     6901 tgcgtagcaa tgctaagtcc ggcattaata gcggcatttt cggcaaaatc accccgttga
     6961 ttcgccgcgt cagcgagaga accaatgacc atgtttccag ctacacctgc tccaggaaca
     7021 agataaccgc ctattgactc tccagcttgc gcgtaggggt ctgtcggtct gtctactgga
     7081 cgataaacat catccaatac ttttggcccc ccaagcccct gactgattgc attaatcaga
     7141 cttgcgccac cctgcaatac gtcaaatggt atgtttacca gaccacgacc agcctgttct
     7201 gcaatttacc ctgcactttg accaccagtg agccaatcgc cagcttgttg catcaatgat
     7261 ggttcttccc gtgttggtgc attattggcc tgattaactg tttgttgctg aacaggtttc
     7321 ggtgcgacag aacttacggg ttgaggtggc tgctgactgg ctgcctgctg ctcaatctga
     7381 gcaaatggat tatttgggtc tgactgcaca cctgatgccg atacttgttc ggatgactgt
     7441 acttccagtt gtgcaaacgg gttgttaggg tcttgctgag gatgtacctt tgcagaggtg
     7501 gcgcgctgtt cgactgttga gtctgtcacc gggtcaccag cccattgagc aaagcgatca
     7561 tcaacgtaac cgcggccttc aggtcctggc gtatattcac cacgctttgc cttcataacg
     7621 ttgccgggac cgtcgtgata agcctgaaga gcgtcacgcc agttaccaaa ctgctggtat
     7681 atctttgcca gatagcgcgc gccagcgtca gcctgatatt cggggttttg catttgctca
     7741 tcggtataac ccatatcacg ccatgtccca ggcatgacct gagtcaatcc tacagccccg
     7801 gcggagctta ctgcggcagg gttgtaagaa gactccttgg cacccagtgc agtcatcaac
     7861 ccttctggca caccgtaacg tgcgccagcc tgctctaaca aatcacggta attagccatt
     7921 tactgcccca aagatggaag atatccgtag cgattaatga agtcgattga cagctcgggg
     7981 tgctgcttca ggtaatctat agaagcctga ggcgcttcca ctcgcttgat accgttttgc
     8041 tgaacgtact taccaaccgc ctcattacgc ttctggttga gcgtgttcag gatgacgcca
     8101 gcgttgcgac gaaaggactc ctcgctctgc gagttctgca gcgaaccaac agcctggtcg
     8161 agctttttgc cctcggcatc agaaagtgcg cccatgcctc gcatggcctg aaccgctgtc
     8221 aggtatgctt gggatttaaa ggtatcaagt cgtgcctgag tgtctgcagc ctgtgagcct
     8281 ggaacgttgg ggattactcc acgtaagcct gtaatgctct taagtgaagg agaactaacg
     8341 atatcgttca gagttaacat gctggttgtg agggtgttga tgccgtcttt gtagccatca
     8401 tttagcgctt gctgcttctg ctgcaactgc tggttgttgg ctgctatgcg gctctgtatt
     8461 tcctggcgct tcaggtcgtt agtttctgct gatagcatcc ggtcaaggcg cttattttcg
     8521 ttgttaatgc ggtttgtttc tgcgtccaga ttaatgcgct gctgacctaa attcgcctgg
     8581 atatcttgcc cgcgcattgt gattgcctga ttccgagcgg cggtttgcga atccaggtcc
     8641 tgaccgcgca tggtaacctg gcgaccctgc attttatcct gtaggtcaaa gtatttttcg
     8701 ggtccgagac tgttcatccc caggtgatcg acaaattcgc cgaactgccg cgggttctgt
     8761 tggtacatct gagcgacgtc ctgaggatta acgccaacac gagctaactc accggcgttg
     8821 ttttgcagcc atgattgcat tgcttctgga gacgatgacg caaggcgtgc gccagccgct
     8881 aaggtgccga tagaattacg ctggtcttca tcaatgaatc ccatgccttt acgaacggat
     8941 tcaatctggt ctggatattg agtagccaac tgacgcaaag caccgcgatc accagacgca
     9001 taagcattag cgtacgcctg ctgaaattct ttctgccgct gagcctgctt ttcctgctga
     9061 aacacccc
//
LOCUS       DADLTI010000157         9038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLTI010000157 DADLTI010000000
VERSION     DADLTI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.157"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="JN755_003413"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="JN755_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY7382087.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1859)
                     /gene="phoA"
                     /locus_tag="JN755_003414"
                     /pseudo
     CDS             complement(445..1859)
                     /gene="phoA"
                     /locus_tag="JN755_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1960..2220)
                     /gene="iraP"
                     /locus_tag="JN755_003415"
     CDS             complement(1960..2220)
                     /gene="iraP"
                     /locus_tag="JN755_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7382088.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2402..2605)
                     /locus_tag="JN755_003416"
     CDS             complement(2402..2605)
                     /locus_tag="JN755_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382089.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2683..3777
                     /gene="ddlA"
                     /locus_tag="JN755_003417"
     CDS             2683..3777
                     /gene="ddlA"
                     /locus_tag="JN755_003417"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7382090.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3801..4013)
                     /locus_tag="JN755_003418"
     CDS             complement(3801..4013)
                     /locus_tag="JN755_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7382091.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4273..4581
                     /locus_tag="JN755_003419"
     CDS             4273..4581
                     /locus_tag="JN755_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7382092.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4640..5734)
                     /gene="yaiW"
                     /locus_tag="JN755_003420"
     CDS             complement(4640..5734)
                     /gene="yaiW"
                     /locus_tag="JN755_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7382093.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDCDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5747..6967)
                     /gene="sbmA"
                     /locus_tag="JN755_003421"
     CDS             complement(5747..6967)
                     /gene="sbmA"
                     /locus_tag="JN755_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7382094.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7319..8476
                     /gene="ampH"
                     /locus_tag="JN755_003422"
     CDS             7319..8476
                     /gene="ampH"
                     /locus_tag="JN755_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY7382095.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8477..8980)
                     /locus_tag="JN755_003423"
     CDS             complement(8477..8980)
                     /locus_tag="JN755_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7382096.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2313 c         2149 g         2336 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt
     1561 aaggcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta
     1621 atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg
     1681 ctaagagaat cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt
     1741 gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct
     1801 tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact
     1861 ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc
     1921 cgtgacaact ttatgacagc tattgaaaag attaactttt tactgacgag gatgcttcaa
     1981 taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg
     2041 ctttacctcg tacagcgccc cctctacctg atcaatcaac cgctgttggt cattttgcgc
     2101 catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc
     2161 tttcgactct tcttcttttt gggcaagctt aaataacaac tcagtaatga gatttttcat
     2221 gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt
     2281 gtctggaagt attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat
     2341 taccagcgaa acgtttctct tatttaaata attacccaca caaaatatag gcttttaatt
     2401 atcaaccttt attataaata aaagctatga aatatattct cttcgttaac ttataattaa
     2461 gagaaaaaaa ctcaccgcat tgccatttaa gccaggaata aattagcttc ccgttcatca
     2521 tttttgtgat cttgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg
     2581 ctttgtcagc agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt
     2641 ggtaatgtat gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta
     2701 ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac
     2761 attgtcgatg ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa
     2821 gggcaatggc acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat
     2881 attgcgttgc gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag
     2941 cttatcgacg cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc
     3001 cacggtacgc tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg
     3061 tttgtaggtt ctgatgttct ggcttcagca acctgtatgg ataaagatgt caccaaacgt
     3121 ctgctacgcg atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt
     3181 cacaacatca gttttgccga agtggagtct aaactggggt taccactgtt tgtaaaaccg
     3241 gctaatcagg gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt
     3301 gccgtcgatc tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt
     3361 cgtgagatcg aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag
     3421 ctcgtactca ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg
     3481 aaagtggtag ttccggcagc cattgcacca gagatcaacg ataagatccg ggcgattgcc
     3541 gttcaggctt atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc
     3601 ccagagaacg aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt
     3661 atgtatccga agctgtggca agccagcggt ctgggttaca ccgatctgat cacacgtttg
     3721 attgagctgg cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat
     3781 tttaacgccc gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata
     3841 atcaaccccg ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc
     3901 caaccttttg cttcaaatcc cagtaccccc accacgccat taagaatgaa tataaggcca
     3961 atggcgaacg catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct
     4021 ccgttaacga aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac
     4081 attttcgtaa ttgttaataa atttaactta aggttgccat tttgtgactc acaacgaaga
     4141 ttcacgcttc gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa
     4201 atgacctgtc agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt
     4261 ccggaggttt ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc
     4321 aatgcctctt caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg
     4381 gctggggcgc aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg
     4441 caggtccagg aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc
     4501 gggctgatcc cgtttttagc aggctgcctc atttttgcag tggtggcgct atggctgcac
     4561 tggcgacatc gccgccagta aaatttacgt tgccagatgc ggcctgaaac gccttatctg
     4621 gcctgacatt cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct
     4681 tcgcaaacca ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag
     4741 gcaacatttc gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct
     4801 tgtacagcgc cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt
     4861 cgctgtcgct catccccagt tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg
     4921 gttctttact gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct
     4981 tactgactgc gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag
     5041 caaagcggta tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagaaccaa
     5101 accacaaccc gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg
     5161 gatacccttt ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc
     5221 gcaccggatt aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt
     5281 caaaaatcgc gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt
     5341 tgccgtttgg cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc
     5401 gttcagcacg acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat
     5461 ctgcctgata atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg
     5521 tcggtgccag cccctgactt tcaaaggtgg tggcgagatc tttcgcccag gcatcgcagt
     5581 ctttcacgct cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc
     5641 cctttttcaa cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca
     5701 atgacagcga agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat
     5761 gggttacttc ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt
     5821 agatagacat caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc
     5881 gaacctgacc aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa
     5941 caattgacgg aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc
     6001 gggcgatgtt gaaatacata tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc
     6061 taaacagctc gcgtaccgta ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca
     6121 gctctttacg gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga
     6181 tccctaccac tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca
     6241 gaccatacgg aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca
     6301 gcaccggcag gaaggcgatc aacgtcatga tggcgttgat aaaactgacg cccatattct
     6361 ccagcgttga agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca ccttcgatat
     6421 gacgcagttg ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca
     6481 cgtagtgact gacaaagaag ttgttcagta cactgatcac caccgcgatc agcgcaatcc
     6541 ccagaaagac gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta
     6601 gcgcggtttg aatcagatca tagaacggcg cataccaggc gttgacggcg accccgactt
     6661 ccaccaaaaa ccaagtgacg aagataatca gcgcagtacc gagtattgac cagtattgcc
     6721 aacggtgcgg gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt
     6781 aagcgtaaaa aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc
     6841 cggaagcgcc ggtgatacgt gccacccagt cacccccacc ggcttgccag aagataacgg
     6901 caatcaatgc ccaaacaaag gccgagagaa aaaacgttcc cggctttggg aaaaaagact
     6961 taaacatatg cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca
     7021 tgaccgctac aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt
     7081 agttcgccag cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta
     7141 cataagcgga ttaacacgcg caggatcaca aatgcaggca gtggtgagca ggaaaaagat
     7201 cgtgtttacg cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc
     7261 catcaatgag tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt
     7321 gaaacgtagt ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc
     7381 ggcgcagccg atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca
     7441 tattttttac ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg
     7501 cgtctttcgt agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc
     7561 ggtcgtgcgt attgcttccc tcaccaaact aatgaccagt gaaatgctgg tgaaattgct
     7621 cgaccagggg accgtaaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg
     7681 cgtgccgacc tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc
     7741 cctgccccgt gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg
     7801 cgagcaacgc tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc
     7861 ggcgtactct aaccttgcgt tcgacctact ggcagatgca ctggcgaatg cgtccggcaa
     7921 gccttatacc cagttgtttg aagagcagat tacccatcca ttggggatga aagacaccac
     7981 ctacaccccc tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc
     8041 gtgcaataac acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat
     8101 gatgcgctgg atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga
     8161 tcgcatgcag acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt
     8221 ccccggcaaa gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg
     8281 tccgaggatt attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat
     8341 cccgcagaag aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa
     8401 aaatatgagc gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt
     8461 tatccccgca tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt
     8521 tattcatttc gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac
     8581 gcgatatgcg tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt
     8641 cattccagtc aatcattgat aataatgttg cgcgggtttg tttggctggt agatgatatc
     8701 ccgtacaatt tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag
     8761 ccagccccat aatataagga gcctgggtaa taccgataag tacgttttct tctctacgca
     8821 gagagataac tccttctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata
     8881 taacttgctt ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga
     8941 gcttaccaaa ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa
     9001 tgcccgtcaa atccttgacg ggcattattt agattaaa
//
LOCUS       DADLTI010000158         8988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLTI010000158 DADLTI010000000
VERSION     DADLTI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.159"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..923
                     /locus_tag="JN755_003424"
     CDS             <1..923
                     /locus_tag="JN755_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382097.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JN755_003425"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JN755_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7382098.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN755_003426"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN755_003426"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7382099.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JN755_003427"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JN755_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7382100.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JN755_003428"
     CDS             4446..4805
                     /locus_tag="JN755_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7382101.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN755_003429"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN755_003429"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7382102.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="JN755_003430"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="JN755_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7382103.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="JN755_003431"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="JN755_003431"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7382104.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2044 a         2173 c         2430 g         2341 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgccg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ctacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat attttacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaatcaac ataatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcac gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggct
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tttgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gttgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggatcataac tgtactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcacc
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gcgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggct tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaa
//
LOCUS       DADLTI010000159         8914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLTI010000159 DADLTI010000000
VERSION     DADLTI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.160"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JN755_003432"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JN755_003432"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7382105.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JN755_003433"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JN755_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7382106.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="JN755_003434"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="JN755_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7382107.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /locus_tag="JN755_003435"
                     /pseudo
     CDS             complement(5775..7256)
                     /locus_tag="JN755_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JN755_003436"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JN755_003436"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7382108.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="JN755_003437"
                     /pseudo
     CDS             complement(7826..8137)
                     /locus_tag="JN755_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JN755_003438"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JN755_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7382109.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8914)
                     /gene="csgA"
                     /locus_tag="JN755_003439"
     CDS             complement(8649..>8914)
                     /gene="csgA"
                     /locus_tag="JN755_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY7382110.1"
                     /translation="NSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNG
                     KNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2231 a         2309 c         2112 g         2262 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgtgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgttta cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 cgctcttccc ctgcactaaa ataggcatca ccaatattta gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtccta ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 ctctgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctat gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
     8821 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
     8881 ccgccatgct gggtaatagt caagtcagag ttac
//
LOCUS       DADLTI010000160         8839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLTI010000160 DADLTI010000000
VERSION     DADLTI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.161"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(158..1129)
                     /locus_tag="JN755_003440"
     CDS             complement(158..1129)
                     /locus_tag="JN755_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7382111.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN755_003441"
     CDS             complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN755_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7382112.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3036..4046
                     /locus_tag="JN755_003442"
     CDS             3036..4046
                     /locus_tag="JN755_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7382113.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4852..5181)
                     /locus_tag="JN755_003443"
                     /pseudo
     CDS             complement(4852..5181)
                     /locus_tag="JN755_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5299..6024
                     /locus_tag="JN755_003444"
     CDS             5299..6024
                     /locus_tag="JN755_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7382114.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6124..6534
                     /gene="uraH"
                     /locus_tag="JN755_003445"
     CDS             6124..6534
                     /gene="uraH"
                     /locus_tag="JN755_003445"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7382115.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6637..7596
                     /locus_tag="JN755_003446"
     CDS             6637..7596
                     /locus_tag="JN755_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAY7382116.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7742..8830
                     /locus_tag="JN755_003447"
     CDS             7742..8830
                     /locus_tag="JN755_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7382117.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2235 a         2336 c         2254 g         2014 t
ORIGIN      
        1 cgacgcaggt gggaccgtgg tcccagggag ataacctgag cggcgataca ggtgggaccg
       61 tggtcccaga gagaatattc aggccagtta tgttttctgg cctgtgagaa aggaaatgaa
      121 gtaaagggag atcaccgtag actaaagcgt gtccgcatca gactcctggt ttctcaagct
      181 ccttaagaat ggcttctatt ttctctatac actccgcagg aatgcgggac ctatccaggt
      241 taagcaccat tttatcgccc ttatacaatg ctgttgcccc tggagcaaac tgatgacgtg
      301 agctcagatt aactcttgat gcgggagatt gtttcagtac ggatgttaaa agactaatga
      361 cctcttcagc ttcaaagatt agtcccgctt tcttctgatc gtggagagtc tcagcctgct
      421 gcttcagtaa ttcttcttta tctgcaaaag ccttctgaag ggcttcacct gaccgggcag
      481 acagttctcc aggatgtgca aacagggcaa caacggattt aggcaatttg gccgtattga
      541 tacagcgggt aatgatctta cgagagatgt tttccgcatc ggcaagcgca gaaatatttc
      601 ctgcaaactc attctgcagc cggcttgtat aacgcaggcc acgttcataa gcactcgtcg
      661 gccgataatc gttacccagt cgggacaatg cagccatctg ctcatcgtcc agttcaccaa
      721 ccagaacgcg ataatcactt tccgtcagga ttgcggcttt acggcgacgg ctgccgtcgg
      781 caatttctat gacatcagaa acccggcggc cgaaagctgg agtctgctga ccggtcagta
      841 aaaatgaagg aatgagatca tccagagcat cttcggtaag tagttcctga tcgcgctcat
      901 tgcctgacca tacgcgtgaa gcgctctcaa cagagtctcc ccggagcacc tcaagggtaa
      961 atttcacttc ccgtccacat accggaagag agattgcatt accgcgggcc atagcaccca
     1021 cgcgcgcaat taatgaatcc accatcggcg cggccggcgc cggtgatgaa gtatcttcag
     1081 tcgattgact atgtgtggta tgtcttggaa tgacaggagc acgcttcatt atctaatctc
     1141 ccagcgtggt ttaatcagac gatcaaaaat ctcattgcaa acaggctccc agatagagag
     1201 ggcattacgc caggcaccgg ttgaagaacg ttgatcaata gcctgttcaa aaacggttct
     1261 catacggatc tgacctttac cgacttcatc cgtttcgcgc acaacatttt taaggaccat
     1321 gcttccccag gcatcccgaa tttgttcttc catccacggg gactgtgagc cattattatt
     1381 actgtatttg gtaagcagaa tacggacatc tggctcgaag cctttaagat ccacgttctt
     1441 aagcaggtca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcga acagctctgc
     1501 cggagtaggg acaatcagaa catcagcagc gcatacaaca tttatcgtac cgataccaag
     1561 gttgggtgca ctgtcgataa ctatcacgtc ataatcatga gcgacagttt caatggccag
     1621 acggagcatc aggtgcgggt cagcaggtaa tttgccttcg tcaaatttcc ccatcagctc
     1681 ggtttcaatg cggtgcagtg ccagacagga cgggatgata tcaaggccag gccagcaggt
     1741 gggctttata gcgtagctgg cgtcatcctt ttccccaaga taaaagggca ggagagtgtc
     1801 ctcagcatga atatgaagat cgggcaccca tccgtgatac atagaagcag ttccctgtgg
     1861 gtcattgcct tcaacaagca gaacacggag tcctttcaga gcaagatcct gagccaggtg
     1921 aacggatact gaggttttgt aaacaccacc tttgtgagct gcaacgccaa tcaccggcgg
     1981 aaacgcatct tcagcacggc gcagtcgtgt accaaataca tcccgcatgt gattgatttg
     2041 ttcgatggta taaccaacgc gttgctcaac tctcccacgg gtttccatat cagggtgcgg
     2101 cagccggcca gctttctcgg catccctgat agcctgagat gacacgccaa ctaaatctgc
     2161 cgcttcacca atcctccagc gccgggttat tttccgggct tccggactgt catcattaaa
     2221 ctgtgcaatg gcgattgctt tcgtcatttc atgaccagca cttatgcact ggttgagtgt
     2281 atccatgagt cccatactga acatccttta atcatcactt tgcattattt tattaaatct
     2341 tggaatttat tgcaaagcaa caataaaatc gcaaagcaca cgaaaaagtg caaagtgaac
     2401 cacattgggt aatgaaagaa cgcaggggat tacaaaagag tacataccgc agtcaatatt
     2461 aatcactgcc gctcgccgca gtaagcgatc tcacacagtg agaaagcggc gaggaagcgg
     2521 aaaatcacag aactcacaga ttgcacgtac gtcgggttgc taaagatgac tcgggatcag
     2581 ccagttataa tgaaattgac tgcggtatgt aatatattga ctatggtatg atgcttaccg
     2641 catctgtagg aaagacgact cagcataccg cagtcaatgt ttcattgttg tacctgtaga
     2701 tctcaatctt ccagagcgta ttcaggaaaa tcaggccagg ggtctggata tctggtgtgg
     2761 cgcttccggc cttgtatctg ggttattcat ggtctcgatc caataattga tcccaaggag
     2821 atcatagtga gatccaaaag agatccccgg atcgctccag catgcgctgt aactggcttt
     2881 aacgtctgtc tggatcgact gcggaatgta aaatgttgac tgttatatgc ataatctaat
     2941 actgtgatat gcaacggtat ttactgaggt atgtatatcc gttgactgcg atacgtatta
     3001 ctacatcctt ttgtggataa ccgttgagga tcaagatgac gagcgaaaat aatagcttac
     3061 ttctgaacct tcaggaagtt gataagacaa ccggtgaagt tgttaagctg gatgtcaaca
     3121 gcaccagtac cgtgcagcca gtagcgctca tgagactcgg tctcttcgtc ccaactctga
     3181 aatcaacagg gaagagcaag gcgaaccgga aaaacgttac agacgcgact gaggagcttg
     3241 tacagctggc cattgccaaa agcgaaggat acactgacgt taagatcacc ggttcgcgtc
     3301 ttgatatgga cacggatttt aaagtctggc tggggataat tcgctccatg tcggagtatg
     3361 gggtaaaaag tgacaccctg gaactgtcgt tcgtcgaatt cgttaagatg tgcggatttg
     3421 actcccgtcg ttcaaacaaa aaaatgcgcg atcgcatcag taattccctg tttaaactcg
     3481 cctcggttac gctgaagttc cagagcgaga ccaaaggatg gaccacccat ctggtgcagt
     3541 cagcctatta tgacatcaac gaggatatcg ttgagatcaa ggctgaacct aagctgtttg
     3601 agctttacca catggacaga agggtactgc tgcggctgaa agccattgac gccctgcagc
     3661 gcaaggaatc agcccaggcg ctttatacct acatcgaaag tcttccccag aatcctgcac
     3721 ctatttccat gaaacggatg cgtgaccggc tgaatctcac ctcaaatgtc tatacacaga
     3781 accacacggt gcgtaaggcg atggagcagc tgcgtgatat tggttatctg gattacaccg
     3841 agtttaaacg tggccgagcc acctatttca gcgtgcatta tcgcaacccc aaactcatca
     3901 gtagtcctgt gaaggttccg cgcaaagagg aagaagagaa ggcgccagag caaaattatg
     3961 acgaagtcat taaggcgctg aaggctgctg gcatcgatcc tcttaaactg gctgaagccc
     4021 tctcagcgat gaagccagaa aattgactgc gatatgcata tcatgactgc cgtatgccgc
     4081 taaggacatt ccattgactg tgatatgcat cacgcatacc gcagtcaata tttattctct
     4141 atcatgtctg cccggatcga acaatattga ctgtcatatg tgatttcggg tcatgatacg
     4201 cagatattga ctacggaatg cgggtttaat gggggtaatc cctggtctgt tcaccattcc
     4261 atgggggata acatgccaca gtcaattttt cagtaacgct ctatatccct tacgaaaatc
     4321 tcaatcgggt gctgagacgg tcatgttacc tgacggtatc cgttcccctg tgtactgcag
     4381 gtttagcccg gcaggatgag actgcagctg tctgtataac ccggcgccag cttcacactg
     4441 ttaaatgcga tcaggaaacg ggagccgaat ggcagaaagg tgttggctag ggaaggtgcg
     4501 aataagcagg ttatttcttc ccaagctgac tcgctgatta aaatttcgcg gatctgggcc
     4561 gatttttttc ccgcaaacac atcgaatcag cctatttagg ggtcagtttg gagatcggaa
     4621 atttttgtac gttaagcaca ttatttaacc aattcggata cgtaagtgat tgatttatcg
     4681 tcattggcta tcgcttttag gttgtcgctc ttgctgtatc gcatttaggt tatccgatta
     4741 agacaaaatc ccctgaaaac gctgtatagc gcgggagtac accctattgc tacaggtaag
     4801 cctgttcaaa aaacaggctt accgtacaat aattctctat atccaaactg accccaagaa
     4861 ggacaccttc agcgcggccg tggtcggttc gccggtaaag taggctttac cctcgctctc
     4921 attccacttg ttcttcacag ggtcgtagcc gcggttaagc acgctgatgc cgccgtcgct
     4981 gcgcttgccg taggtggcgg taacctgctc taggccgcgt tcaaagcggt tttccaggcg
     5041 agcgacttcg taccatttgc caagatagcg gctggcgtca aaaccggaaa tgggctgcac
     5101 gcccttcggt ggggtagggg atttacaggc taccagcgtc agggcaatgg caacaccggt
     5161 cacaacaggc catagcttca tgagaattcc tttcatttgc acggttggaa attaagtgta
     5221 gtgcaccgcg gcggcgcttc atcccatccg cgaaaattcg tagtatattg agagtattcc
     5281 tcaactctgg acacggacat ggcttactcc atcggcgaat tcgccagact cagcgggatc
     5341 accgccacca cgctgcgggc ctggcagcgg cgctacggct tactcaagcc tcagcgcacg
     5401 gagggcggcc accgccagta cagcgatgag gacgtccagc aggcgctaaa aatcctcgac
     5461 tgggtaaaaa aaggcgtccc gattggccag gtcaaaccgc tgctggagcg ccctacgtcg
     5521 cgcctgacca acaactggct cacgctgcag cagagcatgt tacagcgcct gcaggacggc
     5581 aaaatcgagt ccctgcgtca gatgatttac gacgcaggac gcgagtaccc aagaccagaa
     5641 ctggtcgcga acgtgctgcg tccgctgcgc agtcaggtgt cggcaaacgt cgccgccgcc
     5701 atgaccctgc gggagatcct cgacggcatc atcatcgcct acacctcgtt ttgcttggaa
     5761 ggcgataaaa aagcgccggg cgacaacatc ctgctcagcg gctggaacct gaatgacccg
     5821 tgcgaagtct ggctcgaagc cttaacccgc accgggcaag gccaccgtat cgatatttta
     5881 cccacgccgc ccgccacgct ggccccggag attttcccgg accgccactg gcttctggtc
     5941 acgcgcggta aactcaccgc cgcgcgcaaa aagcagatcg agcagtggca gcagcaggta
     6001 tcgcttgaag tgattatcct ctaatcattt tctcctgcgg gaatcttcac gaaaagttga
     6061 atattttccc gcgccacaaa tgctaacgtt ttcctgtgac cgacaacccc acaggaaaac
     6121 acaatgaaaa aaactgcctc tctgctgatg ctgaccaccc tggcctttgc ccctgccgcc
     6181 tttagcgccc ctgccggcac gctgagcgta cacattcttg accagcaaac cgggatgccg
     6241 ccatcggacg ttaccgtcac gctggaaaaa cagcagcagg acaaatggac cccaattgcc
     6301 agcggcaaaa ctgaccatga cggccgcatc aaatcgcttt atccggagga tcaggatatg
     6361 cagcccggcg tctataaagt ggcctttaaa accgcagatt acttccacgg gaagaagctg
     6421 gactccttct tcccggagat cccggtgctg ttcaccgtaa cccggaccaa cgaaaagctg
     6481 cacatccctc tcctgctgag ccaatacggt tattcaactt acaagggcag ttaagaaacg
     6541 ttgattttaa aggcaaataa aaaataattc cgctaatact tggacaaatt agttatgttg
     6601 tgtaagtttt aaagagaaga acgggaggac cacaccatga caacaaaacc tgtgctcggt
     6661 attagcggat gtttgaccgg gtctgccgtt cgcttcgacg gcggacacaa gcgtatgggc
     6721 tttgtcatgg atgaactggc ccagtgggtg agtttcaggc ccgtctgccc ggaaatggcg
     6781 attggcctgc cgacgccacg cccggccata cgcctgacgc tgacggacag cggcgaaacg
     6841 cagctccgct tcagcaagcc gccccatgat gatataacgc agaaaatggc cgactttacg
     6901 gcggactatc tgccaaaaat cggcgatctg tcgggattta tcgtctgcgc gaaatcgcca
     6961 agctgcggca tggaacgcgt gcggctatat gatgaaaacg gcaaccgggg ccgcaaggag
     7021 ggtgtcgggc tgttcaccgc cgccctgctt gaaacctacc cgtggctgcc cgtggaggaa
     7081 gacggtcggc tgcacgaccc ggtgctgcgg gaaaacttta tcgagcgggt atttgccctg
     7141 cacgagctga acacgctgcg cgctaaaggc ctgacgcgcc gcgcgctgct ggatttccac
     7201 agccgctata aattgcagct tctggcgcac catcaggcgg gctaccgcga gattggcccg
     7261 ttcgttgcgt cgctgcatga atgggaagat ctggacgcgt tcttcgtggc gtaccgcgaa
     7321 aagctgatga ccatcctgaa aaagcccgcc tcgcggaaaa accataccaa cgtgctaatg
     7381 catatccagg gctatttccg taaccagctg aacagccgcc agcgcgtcga gctgcgcgat
     7441 gtgatcctgc actatcgcga cgggctgcta cccatcctcg cgccgttgac gctactgaaa
     7501 cactacatgg ccgaataccc tgaccggtat ctgatgacgc aaaactactt tgatccctat
     7561 cctgatgatt tgggtctgcg tctggcagta acctgacgat aaaaatgcga aggcccgttt
     7621 gacccacaga ggcccaaagc agtaccccgt ttagacgaca ccctcactgt cgtctctttt
     7681 ttgcattcca cacaaaagac tgtaattatg tacaggattg taacgcaaaa agggactgcc
     7741 tgtgatcacc accctgcaca ttcaaaacta ccgctcgatt cgcgaaatgt cgctggagct
     7801 ggaacagctc aacatcgtct ttggcccaaa cggcaccgga aaatccaata tctacaaggc
     7861 gatccacctg atgcacagcg ccgcacaggg gcagttttcg caggcactgg cgaacgaggg
     7921 tgggatttta aaagtgttct gggcaggcaa aacccgcagc gaccagctgc ggcggatgaa
     7981 tttggccgtt gaaaccgaaa cctacgaata cgagctgcag gtaggctttg tcgaaaaact
     8041 gccttacccc tcacagtttc aactcgatcc ggtgatcaaa gaagagtcca tctggctgag
     8101 tggtcaatac cgccgcccgt cgtcacagct gatgaagcgt aaaaatcagg ccgtatttct
     8161 gaacaacgtg catcacgaga aagtcaccca cagcggcacg ctgtatgaga acgaatcggt
     8221 gttcggccag cttggcgagc cgcatcttta cccggaagtg tcgcagatgc gcgagtcgct
     8281 gcgtaactgg cgcttttatc aggagttttc tgtatcaatc ggctcggcca tgcgcgcgcc
     8341 tcaggtcggt ttccgctccc cagtgctggc cagcgacggc gcaaacctgg cggcggcgtt
     8401 tcagaccatc gtggaaattg gcgacgagct gctgctgatg cgcatcctcg accaggcctt
     8461 ccccggctgc gtgttttaca gcgacaacac cggcggacgc ttccggatga tgatgcagcg
     8521 tgaagggcta agcagaccgc tggagccggc ggaattctcc gacggcactc tgcgttttct
     8581 gtgcctggcg gtggcgctat taagcccgcg tccaccggcc tttatcgccc tcaacgaacc
     8641 ggaaaacagc ctgcatccgc agatgctgcc cgcgctggcg agcttaatcg ccgaggccag
     8701 ccgctactcg caaatttggc tgaccagcca ctcgccggaa ctggcgaatt taattgagaa
     8761 acagagatcg ttttcgctgt atcagctgtc gatggtggaa ggagaaacga aggtggagag
     8821 gctggggtag tctcccaga
//
LOCUS       DADLTI010000161         8648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLTI010000161 DADLTI010000000
VERSION     DADLTI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.163"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..323)
                     /locus_tag="JN755_003448"
     CDS             complement(<1..323)
                     /locus_tag="JN755_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7382118.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="JN755_003449"
     CDS             complement(333..881)
                     /locus_tag="JN755_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7382119.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="JN755_003450"
     CDS             complement(881..1588)
                     /locus_tag="JN755_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7382120.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JN755_003451"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JN755_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7382121.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="JN755_003452"
     CDS             complement(2285..2995)
                     /locus_tag="JN755_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7382122.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JN755_003453"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JN755_003453"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7382123.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4414..5634
                     /gene="astC"
                     /locus_tag="JN755_003454"
                     /pseudo
     CDS             4414..5634
                     /gene="astC"
                     /locus_tag="JN755_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5631..7364
                     /gene="astA"
                     /locus_tag="JN755_003455"
     CDS             5631..7364
                     /gene="astA"
                     /locus_tag="JN755_003455"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7382124.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7361..>8648
                     /gene="astB"
                     /locus_tag="JN755_003456"
     CDS             7361..>8648
                     /gene="astB"
                     /locus_tag="JN755_003456"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7382125.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2021 a         2221 c         2370 g         2036 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acataaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt
     4201 ttattctttt tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat
     4261 atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca
     4321 ctggctgaca cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat
     4381 acaaataacg aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac
     4441 tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa
     4501 ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg
     4561 gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt
     4621 aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa
     4681 ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac
     4741 gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt
     4801 ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt
     4861 gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca
     4921 tataacgata ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc
     4981 gagcccatct agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg
     5041 cgtgaattgt gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc
     5101 gggcgcactg gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc
     5161 accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc
     5221 gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg
     5281 gcggtagcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa
     5341 cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc
     5401 agtgaagttc gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg
     5461 caagcgaaac agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt
     5521 ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga
     5581 ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca
     5641 tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg
     5701 gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa
     5761 tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag
     5821 atagcgagac aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg
     5881 atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct
     5941 ataacgcatt gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca
     6001 cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc
     6061 gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc
     6121 gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct
     6181 tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg
     6241 cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg
     6301 tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt
     6361 ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg
     6421 accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg
     6481 ccgggcgtga aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta
     6541 ccggtagcgc caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa
     6601 ttctcgccct tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg
     6661 acgcggctgt ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct
     6721 gcgcccgccg tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg
     6781 ttgccgtcag ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg
     6841 gcgggctgat ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag
     6901 cgatgggcgg acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga
     6961 caccagggat cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc
     7021 cgcttttgcg cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca
     7081 ctcgcttcgg actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt
     7141 tgctggaggc gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta
     7201 ccgcgccatt cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg
     7261 cagattactg cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca
     7321 cgcttaaccc cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa
     7381 tttcgacggg ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc
     7441 ctctacccgt caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact
     7501 gaaaatgaaa gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg
     7561 tccgtttatt ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga
     7621 aaaagttgca cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg
     7681 ggtagccaat gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct
     7741 caccgttgcc aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc
     7801 gctgttaaaa gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca
     7861 ggtagcgttg ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg
     7921 tgaaccgggt atgctacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc
     7981 ccgttatccg gcgcgacaga ctcgcgaagc cagcgaggcg gtggcaaggc ttaatcaggt
     8041 gaatccccaa caggtgattt tcgcccagca aaacccggac gttatcgacc agagcgtttt
     8101 tcataatgac gtgattgccg tgagtaaccg ccaggtgctg ttttgccacc aacaggcgtt
     8161 cgctcgccag tcacagttac tggcaaacct gcgggcgcgg gtgaacggtt ttatggcgat
     8221 agaagttccg gcaactcagg tttccgtgtc ggatgcggtg tctacctatc tgtttaacag
     8281 ccaactgttg agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga
     8341 acacgccgga gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga
     8401 actaaaagtc tttgacttac gtgaaagcat ggcgaatggc ggcggcccgg cgtgcctgcg
     8461 gttgcgggtg gtattgacag aagaagaacg ccgggcggtg aatccggcgg tgatgatgaa
     8521 cgataccttg tttaatgcgc tcaatgactg ggtggatcgt tactaccgcg atcgtcttac
     8581 tgctgccgat ctggccgacc cgcaattgct gcgcgaaggg cgggaagcac tggatgtatt
     8641 gagccaat
//
LOCUS       DADLTI010000162         8318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLTI010000162 DADLTI010000000
VERSION     DADLTI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.164"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            332..3181
                     /locus_tag="JN755_003457"
     CDS             332..3181
                     /locus_tag="JN755_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7382126.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JN755_003458"
     CDS             complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JN755_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7382127.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5428..6498
                     /gene="dhaK"
                     /locus_tag="JN755_003459"
     CDS             5428..6498
                     /gene="dhaK"
                     /locus_tag="JN755_003459"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7382128.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6509..7141
                     /gene="dhaL"
                     /locus_tag="JN755_003460"
     CDS             6509..7141
                     /gene="dhaL"
                     /locus_tag="JN755_003460"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7382129.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDRETGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7152..>8318
                     /gene="dhaM"
                     /locus_tag="JN755_003461"
     CDS             7152..>8318
                     /gene="dhaM"
                     /locus_tag="JN755_003461"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7382130.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2173 a         2066 c         2076 g         2003 t
ORIGIN      
        1 cataactaat taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg
       61 taatattttt gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata
      121 actcgatata aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc
      181 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
      241 gatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      301 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      361 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      421 tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg
      481 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc
      541 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      601 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      661 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      721 tcaaacctct gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac
      781 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      841 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      901 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      961 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
     1021 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
     1081 cgatctcgga acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag
     1141 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1201 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1261 gggcggtggc ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1321 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1381 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1441 tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc
     1501 aggagccaga gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt
     1561 cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc
     1621 caccagccgc atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg
     1681 tgggctaatc aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga
     1741 taatgtcaat ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag
     1801 taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg
     1861 gtcaactgcc ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt
     1921 tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt
     1981 gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga
     2041 ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt
     2101 cagcgcgtct tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac
     2161 taactgggag ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac
     2221 accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac
     2281 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg
     2341 tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt
     2401 gcgcagttat gggggaagct tggactcctt tgccagtggc aaactgagcg gctttgacat
     2461 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2521 tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac
     2581 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag
     2641 cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2701 caactgcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca
     2761 gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac
     2821 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa
     2881 tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg
     2941 caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     3001 cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     3061 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta
     3121 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta
     3181 agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa
     3241 atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact
     3301 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa
     3361 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3421 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg
     3481 tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac
     3541 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac
     3601 aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3661 caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag
     3721 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3781 gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta
     3841 ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag
     3901 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca
     3961 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     4021 ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     4081 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     4141 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4201 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4261 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4321 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4381 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4441 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4501 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4561 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4621 aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4681 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4741 tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4801 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4861 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4921 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4981 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     5041 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     5101 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     5161 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5221 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5281 tttattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5341 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5401 ccctaccgta attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5461 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5521 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5581 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5641 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg
     5701 caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5761 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5821 attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc
     5881 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5941 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     6001 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg
     6061 gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt
     6121 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact
     6181 ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg
     6241 ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct
     6301 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6361 gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc
     6421 ttactgaaag ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc
     6481 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6541 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg
     6601 cgagactggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6661 aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac
     6721 gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6781 cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg
     6841 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6901 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6961 tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac
     7021 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     7081 tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     7141 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7201 tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg
     7261 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7321 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7381 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7441 ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata
     7501 tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt
     7561 taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt
     7621 ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg
     7681 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7741 caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7801 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7861 ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt
     7921 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga
     7981 ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga
     8041 tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     8101 gtcaccatac actgttaggt gatccggaac tgctggcggc ggcaagcgaa ctccttcagc
     8161 atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat
     8221 accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc
     8281 atcgcaccct ggtccacctg acccaaacga aagaagaa
//
LOCUS       DADLTI010000163         8226 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLTI010000163 DADLTI010000000
VERSION     DADLTI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.165"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..825)
                     /locus_tag="JN755_003462"
     CDS             complement(<1..825)
                     /locus_tag="JN755_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7382131.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGM"
     gene            1104..2057
                     /gene="tal"
                     /locus_tag="JN755_003463"
     CDS             1104..2057
                     /gene="tal"
                     /locus_tag="JN755_003463"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7382132.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2172..2759
                     /gene="mog"
                     /locus_tag="JN755_003464"
     CDS             2172..2759
                     /gene="mog"
                     /locus_tag="JN755_003464"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7382133.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2794..3360)
                     /gene="satP"
                     /locus_tag="JN755_003465"
     CDS             complement(2794..3360)
                     /gene="satP"
                     /locus_tag="JN755_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7382134.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3509..4222)
                     /gene="msyB"
                     /locus_tag="JN755_003466"
     CDS             complement(3509..4222)
                     /gene="msyB"
                     /locus_tag="JN755_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7382135.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4248..4652)
                     /locus_tag="JN755_003467"
     CDS             complement(4248..4652)
                     /locus_tag="JN755_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7382136.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            5029..6945
                     /gene="dnaK"
                     /locus_tag="JN755_003468"
     CDS             5029..6945
                     /gene="dnaK"
                     /locus_tag="JN755_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7382137.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7034..8164
                     /gene="dnaJ"
                     /locus_tag="JN755_003469"
     CDS             7034..8164
                     /gene="dnaJ"
                     /locus_tag="JN755_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7382138.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         2183 a         2055 c         2159 g         1829 t
ORIGIN      
        1 cattcccgcc tcattggaaa acatactgcg ctgaaaaccg ttagtaatcg cctggcttaa
       61 ggtatatccc gccgcgccgc ctgccgcttc ctgccagcca aaagcactct caaaaataga
      121 ccaaatgacg tggggaagtt gcccgatatt tattacgcaa atcaccaggc tggtcagtac
      181 ccagattatc gccatcaacg ggacaaaccc ctgcatgagc cgggcgacgc catgaagacc
      241 gcgagtgatt gccagcagag caaagacagc gagaataatg cccgtcacca gcggaggaaa
      301 atcaaaagaa aaactcaggg ctcgggcaac ggcgttcgcc tgaactccgc tgaaaattat
      361 gccgtaggcg atgagcaaaa agacggcgaa cagaacgccc atccagcgca tccccagccc
      421 gcgcgccata taccatgccg gtccgccacg aaactgccca ttgacgtcac gttctttata
      481 aagttgtgca agggaacatt cggcaaacga ggtcgccatg ccgataaacg cagcaaccca
      541 catccaaaag acggcacctg gtccaccggc ggtaattgcc agcgcaacgc cggccaggtt
      601 gccgctaccc acgcgcgccg caagactggt acacaatgac tgaaatgagg ttaaaccgcc
      661 tggctgtgga tgaatgctat ttttaagact tttgccaaac tggcggatgt agcgaaactg
      721 cacaaatccg gtgcgaaaag tgaaccaaca acctgcgccg aagagcaggt aaatcattac
      781 cgatccccaa aggacgctgt taataaagga gaaaaaatct ggcatgcata tccctcttat
      841 tgccggtcgc gatgactttc ctgtgtaaac gttaccaatt gtttaagaag tatatacgct
      901 acgaggtact tgataacttc tgcgtagcat acatgaggtt ttgtataaaa atggcgggcg
      961 atatcaacgc agtgtcagaa atccgaaaca gtctcgcctg gcgataaccg tcttgtcggc
     1021 ggttgcgctg acgttgcgtc gtgatatcat cagggcagac cggttacatc cccctaacaa
     1081 gctgtttaaa gagaaatact atcatgacgg acaaattgac ctcccttcgt cagtacacca
     1141 ccgtagtggc cgacactggg gacatcgcgg caatgaagct gtatcaaccg caggatgcca
     1201 caaccaaccc ttctctcatt cttaacgcag cgcagattcc ggaataccgt aagttgattg
     1261 atgatgctgt cgcctgggcg aaacagcaga gcaacgatcg cgcgcagcag atcgtggacg
     1321 cgaccgacaa actggcagta aatattggtc tggaaatcct gaaactggtt ccgggccgta
     1381 tctcaactga agttgatgcg cgtctttcct atgacaccga agcgtcaatt gcgaaagcaa
     1441 aacgcctgat caaactctac aacgatgcag gtattagcaa cgatcgtatt ctgatcaaac
     1501 tggcttctac ctggcagggt atccgtgctg cagaacagct ggaaaaagaa ggtatcaact
     1561 gtaacctgac cctgctgttc tccttcgctc aggctcgtgc ttgtgcggag gcgggcgtgt
     1621 tcctgatctc gccgtttgtt ggccgtattc ttgactggta caaagcgaat accgataaga
     1681 aagagtacgc tccggcagaa gatccgggcg tggtttctgt atctgaaatc taccagtact
     1741 acaaagagca cggttatgaa accgtggtta tgggcgcaag cttccgtaac atcggcgaaa
     1801 ttctggaact ggcaggctgt gaccgtctga ccatcgcacc ggcactgctg aaagagctgg
     1861 cggagagcga aggggctatc gaacgtaaac tgtcttacac cggcgaagtg aaagcgcgtc
     1921 cggcgcgtat cactgagtcc gagttcctgt ggcagcacaa ccaggatcca atggcagtag
     1981 ataaactggc ggaaggtatc cgtaagtttg ctattgacca ggaaaaactg gaaaaaatga
     2041 tcggcgatct gctgtaatca ttcttagcgt gaccgggaag tcggtcacgc tacctcttct
     2101 gaagcctgtc tgtcactccc ttcgcagtgt atcattctgt ttaacgagac tgtttaaacg
     2161 gaaaaatctt gatgaatact ttacgtattg gcttagtttc catctctgat cgcgcatcca
     2221 gcggcgttta tcaggataaa ggcattccag cgctggaaga atggctgaca tcggcgctaa
     2281 ccacgccgtt tgaactggaa acccgcttaa tccccgatga gcaggcgatc atcgagcaaa
     2341 cgttgtgtga gctggtggat gaaatgagtt gccatctggt gctcaccacg ggcggaactg
     2401 gcccggcgcg tcgtgacgta acgcccgatg cgacgctggc agtagcggac cgcgagatgc
     2461 ctggctttgg tgaacagatg cgccagatca gcctgcattt tgtaccaact gcgatccttt
     2521 cgcgtcaggt gggcgtgatt cgcaaacagg cgctgatcct taacttaccc ggtcagccga
     2581 agtctattaa agagacgctg gaaggtgtga aggacgctga gggtaacgtt gtggtgcatg
     2641 gtatttttgc cagcgtaccg tactgcattc agttgctgga agggccatac gttgaaacgg
     2701 caccagaagt ggttgctgca ttcagaccga agagtgcaag acgcgacgtt agcgaataaa
     2761 aaaatccccc cgagcggggg gatctcaaga caattagtgg gattcaccaa tcggcagaac
     2821 ggtgcgacca aactgctcgt tcagcacttc acccatcgcc agatagattg cgctggcacc
     2881 gcagatcaga ccaatccagc cggcaaagtg gatgatcgcg gcattaccgg caatgttacc
     2941 gatcgccagc agggcaaaca gcacggtcag gctaaagaaa acgaattgca ggacgcgtgc
     3001 gcctttcaga gtgccgaaga acataaacag cgtaaatacg ccccacagac ccaggtagac
     3061 accaaggaac tgtgcatttg gcgcatcggt cagacccagt ttcggcatca gcagaatcgc
     3121 aaccagcgtc agccagaaag aaccgtaaga ggtgaatgcg gttaaaccga aagtgttgcc
     3181 ttttttgtac tccagcagac cagcaaaaat ttgcgcgatg ccgccgtaga aaatgcccat
     3241 ggcaagaata ataccgtcca gagcgaaata acccacgttg tgcaggttaa gcagaatggt
     3301 ggtcatgccg aagcccatca ggcccagcgg tgccggatta gccaacttag tgttgcccat
     3361 aattcctcaa aaatcatcat cgaatgaatg gtgaaataat ttccctgaat aactgtagtg
     3421 ttttcagggc gcggcataat aatcagccag tggggcagtg tctacgatct tttgagggga
     3481 aaatgaaaat tttccccggt ttccggtatc agacctgagt ggcgctaacc atccggcgca
     3541 ggcaggcgat ttgcagtacg gctggaatcg tcacgcgata ggcgctgccg ctgaccgctt
     3601 taaccccatt taatgctgcg cccacagggc ctcccagccc cgctccgcgc agcaaaccat
     3661 gcccaagtac gctcattgct gcgtgggtgc gtaaaatgcg ggtcagttgg ctggaaagca
     3721 aatgcgacac accttttgcc aataatttgt ctttcatcag cagcggcagc agctcttcca
     3781 gctcattcac cctggcatcg accgcgtgca gaaactcctg cttatgttcc tcgtccattt
     3841 tcttccaggt attacgcaga aattgttcca gtaactgttg ctcaatttca aacgtagaca
     3901 tctctttgtc ggctttcagc ttcaatcgct ttgaaacatc gagcaaaatg gcccgataca
     3961 atttaccgtg tccgcgcagt ttgttggcga tactatcgcc accaaaatgc tgtaattctc
     4021 cggcaatcag ctgccagttg cggcgatgtt gctcgggatg cccttccatc gatttaaaca
     4081 gttcgttgcg catcagtacg ctggagaggc gagttttgcc tttttcatta tgggtgagca
     4141 atcgggcgaa atttgccaac tgttcctcac tacaatgctg aagaaaatcc agatctgaat
     4201 cattcaggta attaacattc attttttgtg gcttctatat tctggcgtta gtcgtcgccg
     4261 ataattttca gcgtggccat atccgatgag ttcaccgtat gaccggaaaa ggtgattttt
     4321 gagacgcagc gtttattgtc gttatcgctg ttaatgttga tccagtcagt ggtttgccct
     4381 tcttttattt ctgaaggaat attcaggctc tgactggcgc tacgggcggc tttgaaataa
     4441 accgatgcac cgcttaactg taaatcgcca tggtcggcag agagttgtat gcgtttcaca
     4501 atgcgacaaa caggaagttt cagcgccaga tcgttggttt cgttacgcgg cattgcaatg
     4561 acgccgagga gtttatggtc gtttgcctgc gccgtgcagc acagcatcag gctaatcgcc
     4621 aggctggcgg aaatcgtaaa aacggatttc ataaggattc tcttagtggg aagaggtagg
     4681 gggatgaata cccactagtt tactgctgat aaagagaaga ttcaggcacg taatcttttc
     4741 tttttattac aattttttga tgaatgcctt ggctgcgatt cattctttat atgaataaaa
     4801 ttgctgtcaa ttttacgtct tgtcctgcca tatcgcgaaa tttctgcgca aaagcacaaa
     4861 aaatttttgc atctccccct tgatgacgtg ggttacgacc ccatttagta gtcaaccgca
     4921 gtgagtgagt ctgcaaaaaa atgaaattgg gcagttgaaa ccagacgttt cgcccctatt
     4981 acagactcac aaccacatga tgaccgaata tatagtggag acgtttagat gggtaaaata
     5041 attggtatcg acctgggtac taccaactct tgtgtagcga ttatggatgg caccattcct
     5101 cgcgtgctgg agaacgccga aggcgatcgc accacgcctt ctatcattgc ctatacccag
     5161 gatggtgaaa ctctggttgg tcagccggct aaacgtcagg cagtgacgaa cccgcaaaac
     5221 accctgtttg cgattaaacg cctgattggc cgccgcttcc aggacgaaga agtacagcgt
     5281 gatgtttcca tcatgccgtt caaaattatt gctgctgata acggcgacgc atgggtcgaa
     5341 gttaaaggcc agaaaatggc accgccgcag atttctgctg aagtgctgaa aaaaatgaag
     5401 aaaaccgctg aagattacct gggtgaaccg gtaactgaag ctgttatcac cgtaccggca
     5461 tactttaacg atgctcagcg tcaggcaacc aaagacgcag gccgtatcgc tggtctggaa
     5521 gtaaaacgta tcatcaacga accgaccgca gctgcgctgg cttacggtct ggacaaaggc
     5581 actggcaacc gtactatcgc ggtttatgac ctggggggtg gtactttcga tatttctatt
     5641 atcgaaatcg acgaagttga cggcgaaaaa accttcgaag ttctggcaac caacggtgat
     5701 acccacctgg gtggtgaaga cttcgacagc cgtctgatca actatctggt tgaagaattc
     5761 aagaaagatc agggcattga cctgcgcaac gatccgctgg caatgcagcg cctgaaagaa
     5821 gcggcagaaa aagcgaaaat cgaactgtct tccgctcagc agaccgacgt taacctgcca
     5881 tacatcactg cagacgcgac cggtccgaaa cacatgaaca tcaaagtgac tcgtgcgaaa
     5941 ctggaaagcc tggttgaaga tctggtaaac cgttccattg agccgctgaa agttgcactg
     6001 caggatgctg gcctgtccgt atctgatatc gacgacgtta tcctcgttgg tggtcagact
     6061 cgtatgccaa tggttcagaa gaaagttgct gagttctttg gtaaagagcc gcgtaaagac
     6121 gttaacccgg acgaagctgt agcaatcggt gctgctgttc agggtggtgt tctgactggt
     6181 gacgtaaaag acgtactgct gctggacgtt accccgctgt ctctgggtat cgaaaccatg
     6241 ggcggtgtga tgacgacgct gatcgcgaaa aacaccacta tcccgaccaa gcacagccag
     6301 gtgttctcta ccgctgaaga caaccagtct gcggtaacca tccatgtgct gcagggtgaa
     6361 cgtaaacgtg cggctgataa caaatctctg ggtcagttca acctagatgg tatcaacccg
     6421 gcaccgcgcg gcatgccgca gatcgaagtt accttcgata tcgatgctga cggtatcctg
     6481 cacgtttccg cgaaagataa aaacagcggt aaagagcaga agatcaccat caaggcttct
     6541 tctggtctga acgaagatga aatccagaaa atggtacgcg acgcagaagc taacgccgaa
     6601 gctgaccgta agtttgaaga gctggtacag actcgcaacc agggcgacca tctgctgcac
     6661 agctcccgta agcaggttga agaagcaggc gacaaactgc cggctgacga caaaactgct
     6721 atcgagtctg cgctgactgc actggaaact gctctgaaag gtgaagacaa agccgctatc
     6781 gaagcgaaaa tgcaggaact ggcacaggtt tcccagaaac tgatggaaat cgcccagcag
     6841 caacatgccc agcagcagac tgccggtgct gatgcttctg caaacaacgc gaaagatgac
     6901 gatgttgtcg acgctgaatt tgaagaagtc aaagacaaaa aataatcgcc ttataaacgg
     6961 gtaattatac tgacacgggc gaaggggaat ttcctctccg cccgtgcatt catctagggg
     7021 caatttaaaa aagatggcta agcaagatta ttacgagatt ttaggcgttt ccaaaacagc
     7081 ggaagagcgt gaaatcagaa aggcctacaa acgcctggcc atgaaatacc acccggaccg
     7141 taaccagggt gacaaagagg ccgaggcgaa atttaaagag atcaaggaag cttatgaagt
     7201 tctgaccgac tcgcaaaaac gtgcggcata cgatcagtat ggtcatgctg cgtttgagca
     7261 aggaggcatg ggcggcggtg gttttggcgg cggcgcagac ttcagcgata tttttggtga
     7321 cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc gcggtgctga
     7381 tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga ccaaagaaat
     7441 ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa aaccaggtac
     7501 acagccgcag acctgtccga cctgtcatgg ttctggtcag gtgcagatgc gccagggatt
     7561 cttcgctgta cagcagacct gtccacactg tcagggccgc ggtacgctga tcaaagatcc
     7621 gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt ccgttaaaat
     7681 cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg aagcgggcga
     7741 gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc acccgatttt
     7801 cgagcgtgaa ggcaacaacc tgtattgcga agtcccgatc aacttcgcta tggcggcgct
     7861 gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag tgcctggcga
     7921 aacccagacc ggtaagctat tccgtatgcg cggtaaaggc gtcaagtctg tccgcggtgg
     7981 tgcacagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc tgaacgaaaa
     8041 gcagaaacag ctgctgcaag agctgcaaga aagcttcggt ggcccaaccg gcgagcacaa
     8101 cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg acctgacccg
     8161 ctaacctccc caaaagcctg cccgtgggca ggcctgggta aaaatagggt gcgttgaaga
     8221 tatgcg
//
LOCUS       DADLTI010000164         8216 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLTI010000164 DADLTI010000000
VERSION     DADLTI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.166"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN755_003470"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN755_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7382139.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..4022)
                     /locus_tag="JN755_003471"
                     /pseudo
     CDS             complement(1803..4022)
                     /locus_tag="JN755_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(4229..4513)
                     /gene="ihfB"
                     /locus_tag="JN755_003472"
     CDS             complement(4229..4513)
                     /gene="ihfB"
                     /locus_tag="JN755_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7382140.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(4673..6346)
                     /gene="rpsA"
                     /locus_tag="JN755_003473"
     CDS             complement(4673..6346)
                     /gene="rpsA"
                     /locus_tag="JN755_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7382141.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(6457..7140)
                     /gene="cmk"
                     /locus_tag="JN755_003474"
     CDS             complement(6457..7140)
                     /gene="cmk"
                     /locus_tag="JN755_003474"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7382142.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(7313..8077)
                     /locus_tag="JN755_003475"
     CDS             complement(7313..8077)
                     /locus_tag="JN755_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7382143.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         2086 a         2168 c         1930 g         2032 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagaatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcaccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg caatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgataat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgctga aacgcaatcc ttgccactgc
     2281 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
     2341 aacatcggct atatatgcaa tattgaatcc agccctcccc ggtgatccag atgttcatgg
     2401 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
     2461 tgcccactat ccccttcagg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
     2521 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacagcag ccactcgtca
     2581 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctccagccac acacattact
     2641 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
     2701 agccattgcc aacattcaac aatgttgatc cacccttccg gcaaactctt taacccctgt
     2761 tccaggatta acggcccgct aaaatgcaca accatcgcgg cgaggatcaa cggaaccgtg
     2821 ataaatgtca ccaagggaat tgcaaataga tttgcaataa acgaggtcag actaatgcca
     2881 tgaaatatga cgatttgcat gggcataagc aggagtgtga ttcccagttg cagatggatg
     2941 agggaaacaa ctgcacgcaa taccggcggc agttgccact caggacaggg aaaccactga
     3001 taccaaaaaa tcagtgccgc gaccgcagcg gcagagagcc ataaactttg cgagagaatg
     3061 gcaacaggat ccatcagcaa aattgccgcc agacaacata tccatacatc ccagccactc
     3121 cactgtcgcc cacttaactt aagcattccc cacgtagcaa gcgccaccac ggtacgcaat
     3181 gcaggaggtt gcattcccgt caaccaggca taaaaagcag cacagcagat tccgccaatt
     3241 aatggtattt gccagtggat ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt
     3301 ccggcagcca gcaacgccgc aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc
     3361 gttccagtat cgcgcatgat atttttgatt tctttgggga cggataaccg ttcccccata
     3421 cctaaaccaa gaataaccgc attccacgga tagggttgca gcgttgtttg tagtgacgcc
     3481 agatactgtg cacgcaggct acaattcggt tcaatgacac ttgcctgcag aaaacggccg
     3541 gtgagcggct gatgctgggc aatggcataa cgctggctat caaagccgcc atcattaagt
     3601 tggccatgaa ctgcacgaac tttgagtttc attgaccatt gttgtccagc acaaaccgct
     3661 tgcggaagtt attcgccata cagcacaagg cctggcgcag ggaatatacg tcgaccttgt
     3721 agataagtaa tttgaccgta atgagtggtc atgccgtcag ttgcagtgat ctcaacaatt
     3781 gcatcctgcg tcgcgccagt taaggtttct cctgcccaca aaatttgctt tgctgataat
     3841 atgccccaaa cgaaaaacag caacgtcagc gcgatataac ggacggtttt aacagggata
     3901 aatgccagta cgcaggcgaa gagagtcaga aacgcaaggg ttaatgtcct aggcaattgg
     3961 ggcaaaatca gcaacggaaa aattccgcaa attatgcata cgccgactgt cgttattttc
     4021 atgcccccct ccctgtttat ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa
     4081 ttttgcgttc cccgaggcac attcagaaga atttaacgaa agggtgaaaa ctgctcaaaa
     4141 ctctgcgagg cagcttccag ttcctgatta gagaattgaa aaaagcaccc gacaggtgct
     4201 tttctcgcgt tcaagtttga gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt
     4261 ctttaccagg tttaaagtga ggaacgtatt ttccttccag ttctacttta tcgccagtct
     4321 tcggattacg tccggtacgt ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga
     4381 tttcaatacg ctcgccctgc gcaagagtcg aggccatatg ctccagcatc tcttttactg
     4441 catcttcaac cgtcttggcg ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt
     4501 ctgacttggt catgattcct ccggttcctt aaaggcaaat taagcggctg ctgttgctgc
     4561 attgattaag ggcggccgta gccgccctta gtgcttgatt acaggacgaa acctgcaatc
     4621 tgtcaagtaa actcaacaaa cttcggaata aaaatcccga agagtcagag aattactcgc
     4681 ctttagctgc tttgaaagct tcagccattg cgttgttgga gaagtttgca tcttcctgtt
     4741 tgttaacagt tgcgattgca tctttctcgt cagcttcgtc tttcgcacga acagacaggc
     4801 tgattgcgcg gtttttacga tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc
     4861 tcagaaccag ggtagcgtct tcaacgcggt cacgggatgc ttcagaagca cgcaggtaac
     4921 cttcaacgcc gtcagccagt tctacggttg cgcctttagc gtcaactgca gttactttac
     4981 cggttacgat agcgcctttc ttgttcagag caacccagtt gttgaacgga tcttctgcga
     5041 gctgtttaac gcccagggag atacgttcac gttctgcgtc aacctgcaga acaactgcag
     5101 cgatttcgtc gccttttttg tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga
     5161 tgtcagacag gtgaaccagg ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt
     5221 cagtgataga cttgatttta ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact
     5281 gctgccacgg gttagctttg cactgtttca gccctaggga gatacgacga cgttcttcgt
     5341 cgatatccag aaccataact tccactacat cgccaacgtt aacaactttg gacgggtgga
     5401 tgtttttgtt ggtccaatcc atttcggaaa cgtgtaccag accttcaacg ccttcttcga
     5461 tttcaacgaa gcagccgtag tcagtcaggt tggtcacgcg accagtcagt ttggtacctt
     5521 ccggataacg tttagcgata gctacccacg gatcttcgcc cagctgtttc aggcccaggg
     5581 atacacgggt acgttcgcgg tcgaacttca gcactttaac agtgatttcg tcgcccacgt
     5641 tgacgatttc gctcggatgc ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc
     5701 cgtcaacgcc gcccagatca acgaatgcac cgtagtcagt gaggttctta acgatacctt
     5761 taacttccat gccttcctgc aggttttcca gcagctgatc gcgctctgcg ctgttttcgg
     5821 attcgataac ggcacgacga gaaacaacaa cgttgttgcg cttctgatcc agcttgatta
     5881 ctttaaattc aagctctttg ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta
     5941 ccagagaacc aggcaggaac gcacgaatac cgttcagctc aacagtgaag ccgcccttaa
     6001 ctttgccgtt gataacaccg gtaacagttt cagcatcttc gtaagctttt tccagcgtga
     6061 tccaggcttc gtgacgttta gctttctcac gggacagcag agtttcaccg aagccgtctt
     6121 ctactgcgtc cagagcaacg tcaacttcgt cacctacctg gatttccagc tcgccctggg
     6181 cgtttttgaa ctgctcagcc gggatggcgg actcagattt cagaccagcg tcaaccagta
     6241 ctacgtcttt gtcgatagca acaacaacgc cacgaacgat agaacccggg cgggtttcga
     6301 tttcttttaa ggactcttca aagagttgag caaaagattc agtcatgttt aatcttcagg
     6361 tttatattta acgtccacct ggctccatgc cggatggggt tgtttaacat acccgctgtc
     6421 attccattgc aacgggggta ctgcaaattc ggtcgcttat gcgagagcca atttctggcg
     6481 cgcgtattgt agcgcttttt caatcacttg ctcaatgctt aaggtggtgg aatccaacac
     6541 taaagcatcg gctgccggaa ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg
     6601 ttctttgatc tcggccaaaa ggcgctcgaa gttaacacta aagcccttct cctgcaactg
     6661 tagcatgcgg cgatgcgcac gttcttccga ggaggcgtca aggaaaattt tcactggtgc
     6721 atcagggaat accaccgttc ccatgtcgcg gccatcggca atcagacctg gtaattcgcg
     6781 aaacgcgcgt tggcgacgca ataatgcttc acgaacgcgt gggaatgccg cgacttgtga
     6841 agccgcattc gccacttcct gagtacgaat ttcgccgctg acatcttccc cttcgaggat
     6901 cacttccaga ttgccattgg tcgacacaaa acgtacatcc agatgggatg ccagcggtac
     6961 cagcgcatct tccgacgcaa catcaacatg gtgatgtaat gccgccaatg ccagtacgcg
     7021 ataaattgca cccgagtcca gcagatgcca ttgcaacgct tccgccatag ccttacacaa
     7081 ggtgcctttc cctgcaccgc ttgggccatc aatggtaata accggggcaa ttgccgtcat
     7141 ctttatctcc ttaaaacagg cgtaccgtta acataaccgc gcgcattata cgcgccgacg
     7201 ccacaaatcg ttaacgttgt gtgcaaaaac cagttttgtc tgtattcaga tggggattta
     7261 ttgcgcaatt atagcgggct ggcagaagac cagcccgaaa gatgacaatg atttacttac
     7321 cagaggcgat acgatcacgg atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa
     7381 acatggactg ggtgcgaccg ccatccaggc tagccagttt ttcaaagctg ccaaccagcc
     7441 cctgggtgct gatgccacgt ttcttcaaca gatcaaaaga gaaatcatct gcttcggact
     7501 ctttatcacg ggagtattta gcattaatag cgccttctgc gatatcaccc aattgtgagc
     7561 gggaaagctg ggaagccacg ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg
     7621 cataagaagc tttcatttca gccagcgagt gacccaacgc gacgtggccc agttcatggc
     7681 ccagaacacc ttcaatttcg ttgtcattca tcatgtccat caggccactg tagacacgaa
     7741 cacagccgtt cgccatcgcc catgcgttga cgtcgctggt catataaacc ttatagttga
     7801 ccggcgtgcc attaatattg ttacccaatg ctttggcgat ttttgccaga cgtttaccgt
     7861 acttgctgga tttacttgcg acttgattgc cgctgtccat ttcggcacag ccctgattgg
     7921 caatcgcttt tgcatcggca tcgcttaatg ttgctgcttt ataggcattt aaaccggagc
     7981 tgatagccat attggtatca ataccgtggg tattttgaca ccctgtcagt aatgctgcag
     8041 aggtcgcaat cgccagcagt aatttagtat tcttcatgtg aatcatccgt tgattaatca
     8101 tatataaata ttatgtatca acaacttaca ttgacaagcg acgctaccat taatcaaaat
     8161 gactcacaag gtccgaaaaa aaacgccgac aatgtgccgt aatgccagtc agttaa
//
LOCUS       DADLTI010000165         8192 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLTI010000165 DADLTI010000000
VERSION     DADLTI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.167"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JN755_003476"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JN755_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY7382144.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN755_003477"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN755_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2435)
                     /gene="chbC"
                     /locus_tag="JN755_003478"
                     /pseudo
     CDS             complement(1088..2435)
                     /gene="chbC"
                     /locus_tag="JN755_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2520..2840)
                     /gene="chbB"
                     /locus_tag="JN755_003479"
     CDS             complement(2520..2840)
                     /gene="chbB"
                     /locus_tag="JN755_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY7382145.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3139..3477)
                     /gene="osmE"
                     /locus_tag="JN755_003480"
     CDS             complement(3139..3477)
                     /gene="osmE"
                     /locus_tag="JN755_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY7382146.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3679..4506
                     /gene="nadE"
                     /locus_tag="JN755_003481"
     CDS             3679..4506
                     /gene="nadE"
                     /locus_tag="JN755_003481"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7382147.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGAQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4736..5623
                     /gene="cho"
                     /locus_tag="JN755_003482"
     CDS             4736..5623
                     /gene="cho"
                     /locus_tag="JN755_003482"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7382148.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5583..6158)
                     /gene="ves"
                     /locus_tag="JN755_003483"
     CDS             complement(5583..6158)
                     /gene="ves"
                     /locus_tag="JN755_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7382149.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6361..6846)
                     /gene="spy"
                     /locus_tag="JN755_003484"
     CDS             complement(6361..6846)
                     /gene="spy"
                     /locus_tag="JN755_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7382150.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7176..8144)
                     /gene="astE"
                     /locus_tag="JN755_003485"
     CDS             complement(7176..8144)
                     /gene="astE"
                     /locus_tag="JN755_003485"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7382151.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2151 a         2054 c         1893 g         2094 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaacg acccctcccc aaagcttaga
     2281 aaaacgttgt taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata
     2341 aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg
     2401 agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa
     2461 tttaaatatt gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat
     2521 taatttgctg cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg
     2581 actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg
     2641 atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct
     2701 ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct
     2761 gcctgtgcgc gcatttttga taccagtaaa gagatagaca tgcccgcaga acaaaacaga
     2821 taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact
     2881 ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt
     2941 tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa
     3001 ttgtttctcc ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa
     3061 gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca
     3121 agctttcaga cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt
     3181 ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc
     3241 tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccgcgagcat ggatcatgct
     3301 cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt
     3361 gacgtctttc accacaggct gtacaaactg gtctttggta cgatcataag ctgtacaacc
     3421 cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt
     3481 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
     3541 cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
     3601 agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt
     3661 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
     3721 ccgcagatta atgctgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg
     3781 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
     3841 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gccaggaaac tggcaacgaa
     3901 tcactgcaat ttattgccgt acgcctgccc tatggtgctc aggctgacga acaagattgc
     3961 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
     4021 gtattggcta gcgagcaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt
     4081 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
     4141 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
     4201 ggtgacggcg gtacggatat taatctgctg tatcgtctca acaaacgcca gggtaaacag
     4261 ttactgacgg cattaggttg cccggaacac ctttataaga aagccccaac tgccgatctt
     4321 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
     4381 gacgactatc tggaagggaa aaacctacct gaacaggtcg ccagaacaat agagaactgg
     4441 tatctgaaaa ccgaacataa acgccgtccg ccaattaacg ttttcgatga tttctggaaa
     4501 aagtaataat ttgcacctat tggattgtgc gaaaaagagt aatttgttca cgccggatgc
     4561 ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt caatacattg caggagtcga
     4621 ataggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcaac ccgcctcttt
     4681 tttaatcgcc tcccgcctgt tacactggat agataaccag cattcggagt caacagtggt
     4741 acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga
     4801 acatttacat tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg
     4861 cgaaagtgac accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct
     4921 ttctcattta cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg
     4981 gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca
     5041 acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa
     5101 tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct
     5161 gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga
     5221 acaaaaactt tgttatggcc tgttgggact ggaaccgtta agtcgcggtc gtgcatgttt
     5281 tcgttcagcg ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca
     5341 tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg
     5401 tgcggtggca ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg
     5461 gctgtggctg ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc
     5521 cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga
     5581 aattactgaa cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt
     5641 ttaccttgcg gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg
     5701 tcggtagtga gcagtttgtc gcccagttgc cacgccccgt taatcacaaa caccaccccg
     5761 ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca
     5821 tccaggcgcg tcataatatt gaaatccatc gacatctgcc ctgccgtcag tttcgctttt
     5881 accacctggt ccgccgtaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagcgg
     5941 tctgcgcttt caaggagcat ctcgccgcct tccagcaacg tcactatcct ttccatgccg
     6001 ggaaacaaag aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa
     6061 tcacgttttg ccggtgggaa agtggaaatt tcgcgcgttt caccggcagc gtttcgccac
     6121 agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt
     6181 tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag
     6241 tggtgatcag gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc
     6301 caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga
     6361 ttattcagca gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc
     6421 aaaattagca ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg
     6481 ggtttccatg tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc
     6541 ttcagctttt gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc
     6601 ttccagcggc ggacgtttca tctggtcacg ctggcctttc atgatttcgt ggatctgctg
     6661 tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt
     6721 gcctttgtgg tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc
     6781 atgggccagg ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt
     6841 acgcatattc tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg
     6901 tgttgacgag attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa
     6961 tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga
     7021 gtgatgagag aatattacaa aacgatgatt ttgcagagat tatgaagaac tataccggat
     7081 gactggtgat aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa
     7141 cttaataccc gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat
     7201 cccgcgcgta atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg
     7261 gttacggtaa aacgttcctc tccgtcctgc gccagcaatg ttcctttctc aaacggcata
     7321 aaattcagcg tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc
     7381 gaaaccaccc gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc
     7441 agcgcagcaa ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc
     7501 aacgctttgc caagttccaa cgtacaggcc agcgcgccaa aatgtctgac gctgaaatgg
     7561 gtaaacgtac caccaggctc ctgatggaac accagcgcct ccagccccgc cgcacccagc
     7621 cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga
     7681 tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc aacgcacaga ttctttgccc
     7741 tggtcataaa aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt
     7801 tcagcaaata gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt
     7861 ttcccttgct tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg
     7921 atctcgctgt gagatatcgc gccaagcaac gcgtccagca tttccacagg tgccgtctca
     7981 ttaccgtgga tccccgcaga aatcaccagc gcgccttgcg gtggcgttaa tggcgtcagt
     8041 tccagcacac catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata
     8101 accggttttt tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag ta
//
LOCUS       DADLTI010000166         8157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLTI010000166 DADLTI010000000
VERSION     DADLTI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.168"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(61..600)
                     /locus_tag="JN755_003486"
     CDS             complement(61..600)
                     /locus_tag="JN755_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7382152.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JN755_003487"
     CDS             complement(665..1213)
                     /locus_tag="JN755_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382153.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JN755_003488"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JN755_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7382154.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JN755_003489"
                     /pseudo
     CDS             complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JN755_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4823..5539
                     /gene="nanC"
                     /locus_tag="JN755_003490"
     CDS             4823..5539
                     /gene="nanC"
                     /locus_tag="JN755_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7382155.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5559..6665
                     /locus_tag="JN755_003491"
     CDS             5559..6665
                     /locus_tag="JN755_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7382156.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6730..7710
                     /locus_tag="JN755_003492"
     CDS             6730..7710
                     /locus_tag="JN755_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7382157.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2422 a         1744 c         1699 g         2292 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggccgg tatttgctgc
     3301 tttaaggagt gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt
     3361 attcctcatc gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata
     3421 atccaacatt tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt
     3481 aagtggacca gttcttctga gcgaacttaa atggagtagc gatagttact tataataact
     3541 ttgccataca aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat
     3601 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
     3661 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac
     3721 tagccgtctc aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat
     3781 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
     3841 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat
     3901 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca
     3961 ctacgactta agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
     4021 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
     4081 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
     4141 atggcgagta aacccttatt ttcaccactc tcactgagtt agcttgcaaa tctttctttg
     4201 tttagattct aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg
     4261 aagaccaccg caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga
     4321 catattttgt gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
     4381 ctggtataac aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc
     4441 tatggcaaac atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa
     4501 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg
     4561 agtaagtgat ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact
     4621 caggtattta atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga
     4681 gaccttatac aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc
     4741 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     4801 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     4861 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     4921 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     4981 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     5041 cgcactcaat gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac
     5101 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
     5161 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     5221 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     5281 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     5341 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
     5401 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     5461 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     5521 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
     5581 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     5641 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     5701 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     5761 ctgcattccc tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt
     5821 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     5881 acaaatacaa ccccaaaacc aatcgctggg ttaaattgat gtcgcacgcg ccgatgggca
     5941 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     6001 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     6061 tagataaaat caacgcccac tattttgaca aaaaagaaga atattatttc ttcaataagt
     6121 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     6181 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     6241 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     6301 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     6361 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     6421 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     6481 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     6541 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     6601 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     6661 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     6721 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     6781 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     6841 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     6901 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     6961 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     7021 catattgcac ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg
     7081 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     7141 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     7201 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     7261 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     7321 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     7381 actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     7441 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     7501 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     7561 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     7621 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     7681 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     7741 gttcgttctc agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg
     7801 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     7861 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     7921 cttttcgatt atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg
     7981 ccacagcgtt cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc
     8041 tggcaaatag acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata
     8101 ttattgcttg cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcat
//
LOCUS       DADLTI010000167         8057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLTI010000167 DADLTI010000000
VERSION     DADLTI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.169"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1242)
                     /locus_tag="JN755_003493"
     CDS             complement(<1..1242)
                     /locus_tag="JN755_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY7382158.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JN755_003494"
     CDS             complement(1239..2117)
                     /locus_tag="JN755_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7382159.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JN755_003495"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JN755_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JN755_003496"
     CDS             complement(3022..3255)
                     /locus_tag="JN755_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382160.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JN755_003497"
     CDS             complement(3252..4109)
                     /locus_tag="JN755_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7382161.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JN755_003498"
     CDS             4134..4463
                     /locus_tag="JN755_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7382162.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JN755_003499"
     CDS             4453..4761
                     /locus_tag="JN755_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7382163.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JN755_003500"
     CDS             complement(4803..4979)
                     /locus_tag="JN755_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7382164.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JN755_003501"
     CDS             complement(4976..5386)
                     /locus_tag="JN755_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7382165.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JN755_003502"
     CDS             complement(5383..5661)
                     /locus_tag="JN755_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382166.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..7996)
                     /locus_tag="JN755_003503"
                     /pseudo
     CDS             complement(5736..7996)
                     /locus_tag="JN755_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
BASE COUNT         1919 a         2199 c         1845 g         2094 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcac ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaacgctc cagagccttt tacgtccatt ttcccggttg
     6061 gtttttcttc gctgtctcct tttcgcgagt gagtaacgag aatgacgtgg gagtttgttt
     6121 tgtttttgaa gtcgcaaatc gagtcaacaa acgccttctg cccgttatag tcatcgtcgc
     6181 ctatgccaca tttcatcagg ctgtcgatga tgaataactg gatcccgtat cggcggcgag
     6241 cgtagtcgaa tatttcgatc agcctgtcgg ctttcgccgt tccagtcagg ccaaacaccc
     6301 aaagtctttc gtcataaaat ttaaatgcag agtcaatttc cagcactggc ggcatcttgc
     6361 agcacgttgc ctgacgggta agtcgcttaa ggagaatacc aggcttcagc tcaagtgacg
     6421 cgatgcacgt cttcacaccc tgacgcattg cctcaagtgc catatgcccg acaacctcgg
     6481 tgttatgcgt cgccacatat cccctggtta catacaggtg gcgagggtga tcgaccataa
     6541 tacacaagca ttctgcattg ccgatttttt caacattcct gacgaatacc ccacagccct
     6601 cgaatcgatg cgcagttaat ctgccattga gtcgtggtga acggatggcc tccctgattt
     6661 ctcttgtcag tctgattctt gcctcgtatg aatccatccc atgccgctta tcgtccttgt
     6721 atgtgtatgt cacgccagtt ttaactcgcg tccggcatga cccgccgagt gagttaacca
     6781 gttgaacaac cccattgcgc agttcttcac ttgctgagga aaaaacaagc gttccatcct
     6841 tctcgacata cccatccgtt tcaagcagac cacacagcat gcctatacgc gttgatttat
     6901 ttgcggaaaa aaacacacga gggatgaatt tgttttttgc tgtgcacccc attagtccat
     6961 aacctcgcag ggtctccatg agtggattta cctgaccacg cgccgttgat atcagccagt
     7021 ccttaccatc tccagagaag ttgtaatcag gcagttcagc cttcatacgc tcgatcatgt
     7081 atggctcgac gtttgaaaac ttcacgctcc cattgctaag actaccatcc ccgagaaggg
     7141 agccgataac ccatgctaat ggctctgagt ggtcgccaaa gtcaccagtt atttcaggaa
     7201 tcctaacgcc atttttgtgc ctcttcgttt cactcaaccg cttcaacccg aaggtatcta
     7261 tcacgcggcg tttctcgcct ttcgtgaatc cacgactagt gacctcccat aggtgatcgc
     7321 ctgcgcaatc aacataacga ccatcttcaa atgtgactcg gtaaacatct ctaacaccct
     7381 gcgggaatat cccagtgaca gttgacggat tgccgtctac tgacgccacc tgatcgccaa
     7441 tttttacatt cccgtgagta gtccatgtcc catcagctaa aagaataggc tcattcagtg
     7501 ggcatgcctt tccgtgaccg ttcacaccat tgaccagcgt caactctgcc tcacggaact
     7561 ggaatttatc tgccagagat tcccacggtg gattaaacag atactgctgc ttgccgtaga
     7621 aagcgttgat agtgtcctgg taaaactctc gcgcactgta gagttcttca ggatcgaagt
     7681 aggatgccgt gccgatgtac tgccagattt catcctcggt aacaccgttc atcaggcatt
     7741 cgttgatgtc tttgtacggc agagtaacaa gacggcaacg atgttcaccg agtcggcttg
     7801 cgatttccct tgcggcttca cgaccaacat catcaacgtc catcgagatg aatatttcct
     7861 caaacctgtc gaggttatga tactcaaact caatccactg ttgcttagcg cctttcccgc
     7921 caccaaacgg cacggataac gccgagatgc cgtattgcgc atagctcata caatcaattt
     7981 cgccttcgca aagcacaacc gccctcacgc cagcgtccag agcctgccat ccgaacaggc
     8041 aaggttcgca atcacct
//
LOCUS       DADLTI010000168         8049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLTI010000168 DADLTI010000000
VERSION     DADLTI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.170"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..406)
                     /locus_tag="JN755_003504"
     CDS             complement(<1..406)
                     /locus_tag="JN755_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382167.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLE"
     gene            complement(492..1118)
                     /gene="upp"
                     /locus_tag="JN755_003505"
     CDS             complement(492..1118)
                     /gene="upp"
                     /locus_tag="JN755_003505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7382168.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            1443..2480
                     /gene="purM"
                     /locus_tag="JN755_003506"
     CDS             1443..2480
                     /gene="purM"
                     /locus_tag="JN755_003506"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7382169.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2480..3118
                     /gene="purN"
                     /locus_tag="JN755_003507"
     CDS             2480..3118
                     /gene="purN"
                     /locus_tag="JN755_003507"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7382170.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            3290..5356
                     /gene="ppk1"
                     /locus_tag="JN755_003508"
     CDS             3290..5356
                     /gene="ppk1"
                     /locus_tag="JN755_003508"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7382171.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            5361..6902
                     /gene="ppx"
                     /locus_tag="JN755_003509"
     CDS             5361..6902
                     /gene="ppx"
                     /locus_tag="JN755_003509"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7382172.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6941..>8049)
                     /gene="pdeF"
                     /locus_tag="JN755_003510"
     CDS             complement(6941..>8049)
                     /gene="pdeF"
                     /locus_tag="JN755_003510"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7382173.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         2028 a         2014 c         2012 g         1995 t
ORIGIN      
        1 gctccagacc gatgacggca acgattgcgc ccattgccgc aggtggaaac agcacgtcca
       61 accagccggt ccccgctttc ttcacgataa aagaaaccag gcagaacagc acgccgcaca
      121 taataaagcc gcccagcgcg acttcatacc ctaacggcaa cagtaacaat accggtgaaa
      181 taaaggcaaa gctggaacca agataagccg gaattttccc tttacagatg aagagataca
      241 gcagcgttcc aataccgtta aataacagta cagtcgccgg gttaatatga aataagacgg
      301 gcaccaggac ggttgcacca aacatggcga acaaatgttg caaactaagc gggattgtct
      361 gtaaaagtgg cggtctttca ctcaccccga tagcacggcg cgtcatagta ttatcctctg
      421 tattatgtgt tataggcgct ttactcaaaa aaaagccgac tcttaaagtc ggctttaatt
      481 atttttattc tttatttcgt accaaagatt ttgtcaccgg catcgccgag gcccggaata
      541 atgtatccgt gctcgttcag tccctggtca atcgatgcag tatacagttc gacgtccggg
      601 tgcgcttttt ccagcgcagc gataccttct ggcgcagcga ccagcaccag aactttgatg
      661 ctgctgcagc ccgctttttt cagcaggtcg atggtcgcga taacggaacc accggttgcc
      721 agcattgggt caacgatcag cgccatacgc tcatcgatgt tagaaaccag tttctggaag
      781 tacggtaccg gctccagcgt ttcttcatta cggtacatac cgacaacgct gatgcgcgcg
      841 ctcggaacgt tttccagcac accgtccatc ataccaagac ccgcacgcag aattggcaca
      901 acggtaattt tcttaccttt gatctggtcg atttctaccg ggccgttcca gccttcgata
      961 gttacttttt ccgtttcgag gtcggcggtc gcttcgtaag tcagcaggct acccacttcg
     1021 gaagcgagtt cgcgaaagcg cttggtgctg atatcttgct cacgcatcag tcccagcttg
     1081 tgtttgacga gtgggtgttt gacttccacg atcttcatac tctttctcct ttgaggggca
     1141 gccacaaaaa aaatcgaagg attatacctc ctttcttcaa ggcggcaata ttattttcgt
     1201 tgactttagt caaaatgata acagtttgag ataaagttat tttatattca aatggttatg
     1261 aaagaagatt attccatccg aaaactaacc tttgccctgg cacaagtctt ctttcgccgc
     1321 gcgcccgggg aaaagacgtg caaaaaggtt gtgtaaaaca gtctcgcaaa cgtttgcttt
     1381 ccctgttaga attgcgccga attttatttt tctaccgcaa gtaacgcgtg gggacccaag
     1441 cagtgaccga taaaacctct cttagctaca aagatgccgg tgttgatatt gacgcgggta
     1501 atgctctggt tggaagaatc aaaggcgtag tgaagaaaac gcgtcgtccg gaagtgatgg
     1561 gcggtctggg cggcttcggt gcgctgtgtg cattgccgca aaaatatcgt gaacccgtgc
     1621 tggtttccgg cactgacggc gtaggtacca agctgcgtct ggcaatggac ttaaaacgtc
     1681 acgacaccat tggtattgat ctggtcgcca tgtgcgttaa tgacctggtg gtgcaaggtg
     1741 cggaaccgct gtttttcctc gactattacg caaccggaaa actggatgtt gataccgctt
     1801 cagcagtgat cagcggcatc gcggaaggtt gtctgcaatc gggctgttct ctggtgggtg
     1861 gcgaaacggc agaaatgccg gggatgtatc acggtgaaga ttacgatgtc gcgggtttct
     1921 gcgtgggcgt ggtagaaaaa tcagaaatca tcgacggctc taaagtcagc gacggcgatg
     1981 tgctgattgc actcggttcc agcggtccgc actcgaacgg ctattcgctg gtgcgcaaaa
     2041 ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact tgatggtaag ccattagccg
     2101 atcatctgct ggcaccgacc cgcatttacg tgaagtcagt gctggagttg attgaaaagg
     2161 tcgatgtgca tgccattgcg cacctgaccg gcggcggctt ctgggaaaac attccgcgcg
     2221 tattgccaga taatacccag gcagtgattg atgaatcttc ctggcagtgg ccggaagtgt
     2281 tcaactggct gcaaacggca ggtaacgttg agcgccatga aatgtatcgc accttcaact
     2341 gcggcgtcgg gatgattatc gccctgcctg ctccggaagt ggacaaagcc ctcgccctgc
     2401 taaatgccaa cggtgaaaac gcgtggaaaa tcggtatcat caaagcctct gattccgaac
     2461 aacgcgtggt tatcgaataa tgaatattgt ggtgcttatt tccggcaacg gaagtaattt
     2521 acaggcaatt attgacgcct gtaaaaccaa caaaattaaa ggcaccgtac gggcagtttt
     2581 cagcaataag gccgacgcgt tcggccttga acgcgcccgc caggcgggta ttgcaacgca
     2641 tacgctcatc gccagcgcgt ttgacagtcg tgaagcctat gaccgggagt tgattcatga
     2701 aatcgacatg tacgcacccg atgtggtcgt gctggctggt tttatgcgca ttctcagccc
     2761 ggcgtttgtc tcccactatg ccgggcgttt gctgaacatt cacccttctc tgctgccgaa
     2821 atatcccgga ttacacaccc atcgtcaggc gctggaaaat ggcgatgaag agcacggtac
     2881 atcggtgcat ttcgtcaccg atgaactgga cggtggcccg gttattttac aggcgaaagt
     2941 cccggtattt gctggtgata cggaagatga catcaccgcc cgcgtgcaaa cccaggaaca
     3001 cgctatttat ccactggtga ttagctggtt tgccgatggt cgtctgaaaa tgcacgaaaa
     3061 cgccgcgtgg ctggatggtc aacgtctgcc gccgcagagc tacgctgccg acgagtaatg
     3121 cccccgtagt taaagcgcca gctctgccgc tggcgttttt caattcacct gtaaatcgca
     3181 agctccagca gtttttttcc cccttttctg gcatagttgg acatctgcca atattgctcg
     3241 ccataatatc caggcagtgt cccgtgaata aaacggagta aaagtggtaa tgggtcagga
     3301 aaagctatac atcgaaaaag agctcagttg gttatcgttc aatgaacgcg tgcttcagga
     3361 agcggcggac aaatctaacc cgctgattga aaggatgcgt ttcctgggga tctattccaa
     3421 taaccttgat gagttctata aagtccgctt cgctgaactg aagcgacgca tcattattag
     3481 cgaagaacaa ggctccaacg ctcattcccg ccatttactg ggcaaaattc agtcccgggt
     3541 gctgaaagcc gatcaggaat tcgacggcct ctacaacgag ctattgctgg agatggcgcg
     3601 caaccagatc ttcctgatta atgaacgcca gctctccgtc aatcaacaaa actggctgcg
     3661 tcattatttt aagcagtatc tgcgtcagca cattacgccg attttaatca atcctgacac
     3721 tgacttagtg cagttcctga aagatgatta cacctatctg gcggtggaaa ttatccgtag
     3781 cgatagcatc cgttacgcgc tgctggagat cccatcagat aaagtgccgc gctttgtgaa
     3841 tttaccgcca gaagcgccgc gtcgacgcaa gccgatgatt cttctggata acattctgcg
     3901 ttactgcctt gatgatattt tcaaaggctt ctttgattat gacgcgctga atgcctattc
     3961 aatgaagatg acccgcgatg ccgaatacga tttagtgcat gagatggaag ccagcctgat
     4021 ggagttgatg tcttccagtc tcaagcagcg tttaactgct gagccggtgc gttttgttta
     4081 tcagcgcgat atgcccaatg cgctggttga agtgttacgc gaaaaactga ctatttcccg
     4141 ctacgactcc atcgtccccg gcggtcgtta tcataatttt aaagacttta ttaattttcc
     4201 caatgtcggc aaagccaatc tggtgaacaa accactgccg cgtttacgcc atatttggtt
     4261 tgataaagcc cagttccgca atggttttga tgccattcgc gaacgcgatg tgttgctcta
     4321 ttatccttat cacacctttg ggcatgtgct ggaactgctg cgtcaggctt cgttcgaccc
     4381 gagcgtactg gcgattaaaa ttaacattta ccgcgtggcg aaagattcac gcatcatcga
     4441 ctcgatgatc cacgccgcac ataacggtaa gaaagtcact gtggtggttg agttacaggc
     4501 gcgtttcgac gaagaagcca acattcactg ggcgaagcgc ctgaccgaag caggcgtgca
     4561 cgttatcttc tctgcgccgg ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa
     4621 agaaaacggt gaagtggtcc gttacgcaca catcgggacc gggaacttta acgaaaaaac
     4681 cgcgcgtctt tatactgact attcgttgct gaccgcagat gcgcgtatca ccaacgaagt
     4741 acggcgggta tttaacttta ttgaaaaccc ataccgcccg gtgacatttg attatttaat
     4801 ggtgtcaccg caaaactctc gccgtctgtt atatgaaatg gtagaccgcg aaatcgccaa
     4861 cgcgcagcaa gggctgccca gtggtatcac cctgaagcta aataaccttg tcgataaagg
     4921 cctggttgat cgtctgtatg cggcctccag ctccggcgta ccggttaatc tgctggttcg
     4981 cggaatgtgt tcgctgatcc ccaatctgga aggcattagc gacaacattc gtgccatcag
     5041 tattgttgac cgttaccttg aacatgaccg ggtttatatt tttgaaaatg gcggcgataa
     5101 aaaggtctac ctttcttccg ccgactggat gacgcgcaat attgattatc gtattgaagt
     5161 ggcgacaccg ctgctcgatc cgcgcctgaa gcagcgggtg ctggacatca tcgacatatt
     5221 gttcagcgat acggtcaaag cacgttatat cgataaagaa ctcagtaatc gctacgttcc
     5281 ccgcggcaat cgccgcaaag tacgggcgca gttggcgatt tacgactaca tcaaatcact
     5341 cgaacaacct gaataaccct atgccaatac acgataaatc ccctcgtccg caggagtttg
     5401 ctgcggtcga tcttggttca aacagttttc acatggtcat agcccgtgtg gtagatggtg
     5461 ccatgcagat tattggccgc ctgaaacagc gggtgcatct ggcggacggc ctggggccag
     5521 ataatatgtt gagtgaagag gcaatgacgc gcggtttgaa ctgtctgtcg ctgtttgccg
     5581 aacggctaca agggttttct cctgccagcg tctgtatagt cggcacccat acgctgcgtc
     5641 aggcgctgaa cgccactgac tttctgaaac gcgcggaaaa ggtcattccc tacccgattg
     5701 aaattatttc cggtaatgaa gaagcccgtc tgatttttat gggcgtggaa catacccaac
     5761 cggaaaaagg tcgcaaactg gttattgata ttggcggcgg atctacggaa ctggtgattg
     5821 gtgaaaattt cgaacctatt ctcgttgaaa gccgccggat gggttgtgtc agctttgccc
     5881 agctttattt tcctggcggg gtcatcaata aagagaattt tcagcgcgct cgcatggcgg
     5941 cagcacaaaa actggaaact ttaacctggc aattccgtat tcagggctgg aacgttgcaa
     6001 tgggcgcttc cggtaccata aaagccgccc atgaagtgtt aatggaaatg ggcgagaaag
     6061 acgggataat taccccggaa cgtctggaaa aactggtaaa agaaatttta cgtcaccgta
     6121 atttcgcatc gctgagttta ccgggtcttt ccgaagagcg gaaaacagtc ttcgttcctg
     6181 gactggcgat tttatgtggt gtgtttgatg ctttagccat ccgtgaactg cgcctttctg
     6241 acggggcgtt acgcgaaggc gtactgtatg aaatggaagg acgtttccgt catcaggatg
     6301 tgcgtagtcg caccgccagc agcctcgcca accagtatca catagacagc gaacaggccc
     6361 gacgagtgct ggataccact atgcaaatgt acgaacagtg gcgggaacag caaccgaagc
     6421 tggcgcatcc gcaactggag gcgctactgc gatgggccgc catgctgcat gaggtcgggt
     6481 tgaatatcaa ccacagcggt ttgcatcgcc actccgctta tattctgcaa aacagtgact
     6541 tgccgggttt taatcaggaa cagcagctga tgatggcgac actgctgcgc tatcaccgta
     6601 aagcgattaa gctcgacgat ctgccgcgct ttaccttgtt taagaagaaa cagttcctgc
     6661 cactgataca gctattgcgc cttggcgtat tactaaacaa tcaacgtcag gcaaccacca
     6721 caccgtcaac attgacactg attactgatg acagtcactg gacactgcgt ttcccgcatg
     6781 actggtttag tcagaatgcg ctggtactgc ttgatctgga aaaggagcaa gaatactggg
     6841 aaggcgtggc tggctggcgg ttgaaaattg aagaagaaag taccccagaa atcgccgctt
     6901 aatgctttgc gggccgacga gaaatgtcgg cccgcattat tcaggcactt tcgcgaatgg
     6961 gttcgatttc attcagcgta tcaattaacg gttgcggctt accaataaga taaccctgca
     7021 tataatcgat ccccaaagag agcaccgcct cgcggatctc ttcgttttca acgtactctg
     7081 ccactacccg cattttcttc attcgcgcca gatggcaaat cgacgccaca atctgataat
     7141 ccagactatt tgacacaata ttgcggataa agctgccgtc aattttaagc agatcggcat
     7201 tcacattttt aagccgtgca tagctggcgt agccggtgcc gaaatcatca atcgcaatct
     7261 ggcagcctaa ttcctgaaga tgctgcaagg tgatttgcgc ctgcttaaca ttggtcagag
     7321 cattactttc ggtgacttca aaaataagtt gccacgcttc aatctgatat ttagccagca
     7381 actgactgac ttcaacagga aaacgagcct gacataccga ggttggagac agattaatag
     7441 caaaacggtg agcgggcatc ttcgctctgt tttcagccat aaattgcagc gtatgctcaa
     7501 tgacccacat gtcgatactc gacgataaac caaattcgtg cgcgaccggc aagaagctat
     7561 cggggccgat cagttcatca ttctcacctt tcatgcgcag aagaatttca tggtaaacat
     7621 caccacgcat accggtaatc ggctgggcca tcaggaaaaa atggttgtgc tccagcgcct
     7681 gctgtagtcg attcatcatc gcgactttat ctttcaattc gcgttgcaaa tacattgccc
     7741 cgcaacgctg catattttcc ggggcgttgg tcacgatgga aagttcggcg accgtattta
     7801 gctctcccag cagcaggtag atatgattca ctggcgagcg cacatagcag taactgacgc
     7861 caatctgcgg ttgcatcggc atcccatccc aaaagaaacg aaattgcttg agatgactat
     7921 ccagtgcggt aatgcgctcc tggtgcgatt ctgtattcag tcgcagcgcg agatcgttac
     7981 ccgaaagctg ataaacatct tcacccggtt ccagcaaggg tgacagccag tgagaaagtt
     8041 tttgcttgt
//
LOCUS       DADLTI010000169         7893 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLTI010000169 DADLTI010000000
VERSION     DADLTI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.171"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            115..442
                     /locus_tag="JN755_003511"
                     /pseudo
     CDS             115..442
                     /locus_tag="JN755_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(472..3384)
                     /locus_tag="JN755_003512"
     CDS             complement(472..3384)
                     /locus_tag="JN755_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382174.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3387..3503
                     /locus_tag="JN755_003513"
     CDS             3387..3503
                     /locus_tag="JN755_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7382175.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3867..4709
                     /locus_tag="JN755_003514"
     CDS             3867..4709
                     /locus_tag="JN755_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7382176.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4712..5800
                     /gene="capU"
                     /locus_tag="JN755_003515"
     CDS             4712..5800
                     /gene="capU"
                     /locus_tag="JN755_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7382177.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5805..6755
                     /gene="virK"
                     /locus_tag="JN755_003516"
     CDS             5805..6755
                     /gene="virK"
                     /locus_tag="JN755_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7382178.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6820..7764
                     /gene="lpxM"
                     /locus_tag="JN755_003517"
                     /gene_synonym="msbB"
     CDS             6820..7764
                     /gene="lpxM"
                     /locus_tag="JN755_003517"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7382179.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2126 a         1769 c         1872 g         2126 t
ORIGIN      
        1 tgtggaggca cttccgtaaa gaagggcatc aagctcctgc tccgacatac ctgcaggcaa
       61 aggccatgaa agccctgatt tccggaagcg agcgaacagt ccacaaactg ttgaacgtgg
      121 tgtcagcggc ggacagggat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
      181 ctttacccgg tcaaaacagc gttgcggtgc accacgcctg acggatgaac tgcgtgctca
      241 gggttaccac tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg ggctgagggc
      301 aaaggcctcc cggaagttca gcccggtcag ttaccgcgca catggcctgc ccgtgtcaga
      361 aaatctgctg gagcaggatt tttacgccag tggtccgaac cagaagtggg cgggagacat
      421 cacgtactta cgtacagatg aagttcggtt acatcctgtc agcacggaac ctcacgcttt
      481 ttgagcggca cgacggatcc cccccaccca gtatccgaca gacttttcac tgcgttcgta
      541 aaaacccttc ttccctccca gcatatccac taattccggt gacatatcct gcagataagt
      601 ctgcaaatca tgccccagat cttccggtga ctgcaactca taaaccgcgc gccacacatc
      661 accctgcctt attttaagcc ccgggttaag aatatactgt ccgtggttca gcctcagaaa
      721 aagctttttt ccataaggat ttttgctgtt aacttcatgt tgagagagct ggctgctgat
      781 ataactcctt cgtttgcggc gttccggcag caatgcttca ggaagatgaa ccacacactc
      841 tgccagtcgt gccgcactga aggcttccga aaaaaacaga gcacttccca gatgcgtgtg
      901 gaacagcgcc tgcatcagga tcaccagaaa cattgccatc tgccggttgt ccagtttgat
      961 gaggcgtcct tccacctgca gtgacagact ttcaggtttc agcagggtat acaactgcgc
     1021 acttttctgt tgcgcataac ggggcgtact gaccgccgct gacagaactt gatgataagc
     1081 cgccagcccg tcattaccgg tcagcaaggg atctgcacca cgctccagca gtaactgaac
     1141 cagtgcaata ttacctgccc gggctgctga catcagcgga gtaagattaa acggcgaacg
     1201 gtgatcaaca ccataacgtt caatatcccg caacacggca accgggttat tcattgagta
     1261 tggccggaaa tacttcagct ccagtaactg cagagtcttc gttctgggct gccttgtggg
     1321 cttaaaatct tcccggtaca gtgcattata cagcgggaac agggaataaa tcagactgta
     1381 ctccagcgcc tgcagttgta tggttttatc ggctgtacct gcgaggatat gctgtttcca
     1441 ctgtcgtgca tcctctgcgg tgataatctg ccatggcatc tcagatgtct gcaggatccg
     1501 gctacggatg gtgtccgcct gctcctgctt cccctgtttt tcaagcagat gtgcggtttt
     1561 ctgccactca tcacggctgg aaatctcttc ctgcagattc agaggctctt cctgatgcgt
     1621 gagcgcaagc agactccaca gaggatgctc cacctgctgt tcaacaagat acacattgtg
     1681 tgtagcacgg gtgagcgcaa catacagcgc gttggtaaaa aatttataga tttccgctga
     1741 acgatcctgc ctgtcccgcg gacgggaata acgggcctcg ccctcaagat cagccggtgt
     1801 aagaccctcg caaatatcat caaatgcctg ccgggcagcg cttactatat tgtaaaggat
     1861 caccgtttcg tattccagcc ccttaacctc ctgaaccgaa aagaccagcg gcgtactgaa
     1921 ccagcatcgc gcctgtgctt tctgttccgg atgcatcaca atgaccgcta cacgattcga
     1981 aagtgaggtt ttggcattca gttcctgctt tgtctcttcc ctgtcgtcca gcaaccggat
     2041 gctcccttct gcctgaccgc agcttctgac aaaatggtga ctttcccgat caatagcact
     2101 aaagcggacc tgtttgagtc tcagcaaccg gttagccagt gcagtgacgt ggtgcccgtt
     2161 acggtaattc gcctgcagga tattaacggt tgtgtcgttt ccctgttgct gccggaaaaa
     2221 gagacttttg agcgaactcc atgagaaaaa gctcggatga actatctgat tggcatcacc
     2281 acagagtaaa aactgaccgg gatgacgtag cgttttcagt accagttgca gttgaacagg
     2341 cgtcatatcc tgaacttcat caacaaaaat gacatcataa cggggagttg ccaacgaaag
     2401 atactgatga ctgagcagat tactgtcata ctgatgcgac tgtttcagcc atgcgatata
     2461 acgttcaaac aggacataga ccgtcgggcg gtcctccatg ccatagagcg actgccgtat
     2521 ccccagtgac agatacgcct cccgggacag cggcccattc ccggaggcaa ccgcaccgat
     2581 cacgcccctg aactcctcat acagggtatg ggcagccccc agtgcggcga tagccctgtt
     2641 gcggggcagc cagtcgctga acgcactgaa agtcacctcc ctgccttccg gaatgcgcag
     2701 agtttccaga aattccgtca gcgaaagaaa gtcaatattc tgtacctcac tcccttcccc
     2761 tgaagcgtca tagagagtac gcgctttttc caccagaaat gaggaaaggc tgagatacag
     2821 tatatcacca gcagcctgct tcattttttc cagtaccagc gccgtcttac cacttcccgc
     2881 cgagccaaca atcaccagcg gtagcgggtg ttcgtacagt gcctgctgat catcatcaaa
     2941 ggacagcatt ttatccaggc ggtggaaacg cccatgagac ggattgatat acgtcagcgc
     3001 ttcagtcgcg atatcaacag gatccggcac tggttgttgt tgtaagcgcc cttcatcaat
     3061 caccacgttt ctgcgaagaa aacgtgaagt gttataggca tgattgcgaa tatattccag
     3121 aacaaggcag atagtttcac cgtggtagcg atacagcgaa aacagtaatc ggtcccggat
     3181 actgagccgg gcgcggtaaa gattggtgtc aattttacga acatcagcct gaacaaagtt
     3241 atcggtggca atagcctgtg caaatttttt ataacccggg atcgtgtcag gattcagagt
     3301 atgataaaga cagattttca tggctgccgg tttccgtcgt gcaaaaaaca tgctgatttt
     3361 acgaaggaat gccttactga acaaccttgc ccggaatacc ccccgaaatc agatggaaca
     3421 atcagaaaaa tgctctgcag ttatggaata aagaaggtgg ggtggtacgc cgggagggcg
     3481 atccgctgga aatggcgcag tagctgcagg cctgtcatga tgccggaata gaagttcgtg
     3541 tacaggttaa tgaaagcgtc accccgtaaa taaactctgc gctgaaattc ctttttttca
     3601 ttggagcacc tgtgttgttc tgaggtgaac atatcacctc tgttcaggta gccaaattca
     3661 gtgtgccacc tcaaggttct tagtgagtcg tttttaaaag atatgataat atatcattgt
     3721 ttatgaattg ttgatgttta atttagtaaa ttatatcaaa tatgccatgg gtttgctatg
     3781 ttattcatcc agtatctgga aataaaatgc accagcttta ttgggttttc cttttgtctc
     3841 tgcttactag gtgccagaaa ctttaaatgt taaacgaagg gggcattttg ttcaaagcca
     3901 atcatgttcc ggtattaatg tatcatcatg tcagtcattg ccccggactg gttacacttt
     3961 ctcccgtgac attccggaag caaataaaat ggttggctga aaacaactgg aaaacactgt
     4021 catcagatga actggagttt ttttatcggg gcggaaaact cccccggaaa agcgttatgt
     4081 tgacatttga tgatggatac cttgacaact ggtttcaggt atatccttta ttaaaagagt
     4141 ttaatctcaa agcacatatt tttttaatta ccggttttat tgggaatggt ccggttcgtc
     4201 actctcctgg aaaggaatat tctcatcgcg attgcgaaca tcaaatagcg acagggaatg
     4261 ctgataatgt tatgcttcgc tggtctgaag taaacgaaat gttgcaatct ggtctggtcg
     4321 aatttcatgt tcatacgcat actcatacac gctgggataa aaagttcagt tcacgagaag
     4381 aacagtgtaa gcatcttcgg caggacttgt tgagcgggag agaatatttg aaagaaatga
     4441 caggaaaatg cagtaaacat ttgtgttggc cggaaggtta ttataacaag gattatattc
     4501 aggtcgctga agaacttgga ttctattatt tatatacaac agaaagaaga atgaatgctc
     4561 ctgctaaagg aactacacgg ataggaagaa taagcaccaa agaacgggaa agttgcgcat
     4621 ggctgaaaag acgacttttt tattatacaa caccgttttt ttcatcgttg ctggcctttc
     4681 ataaggggcc aagattaccg gatgattaat aatgaatata ctatttacgg aatcatcacc
     4741 gaatataggt ggtcaggaat tacaggctgt tgctcaaatg aaggccctga agaaaatggg
     4801 gcattcagtt ctgcttgtct gcagggaaaa cagcaaaatt gcttttgaag ccagtaaatt
     4861 gggaattgat atcacattcg cgttatttcg aaacagtctt cacatcccta ctgcatggag
     4921 attactcgga atagttcatg gttttcagcc caatgcaatc gtttgtcaca gtgggcatga
     4981 tagcaatatt gttggtttag tacggttatt cactaggaaa catccattca gaattatcag
     5041 gcaaaagaca tatttgacac gaaaaacaaa agttttttca ataaatcatt tttgcgatga
     5101 ggtgattgtt cccggaacaa gtatgaagac acatctggag caggaaggat gtcgaacccg
     5161 ggttactgtt gtgcctccag gctttgattt ccagaaatta tacgttgatt cccgaaacag
     5221 tttgcctcca aatgttcttt cttggctggc gtcccgaagg ggatgccccg ttattgctca
     5281 ggtaggaatg ttgcgcccgg aaaaagggca cgaatttatg ttgaatttac tgttccattt
     5341 aaaaatgaat ggacgacagt tctgttggtt gattgtgggg tctggttcgc ctgaactgcg
     5401 ggagcattta cagtatcaga ttgacagtat gggcatgcat gatgatgttt ttattgctga
     5461 caatgttttt cctgccgccc ccgtatatcg ggttgccagt ctggtggttc tgccttcaga
     5521 aaacgaatcg tttggtatgg tactggcaga agcatcggca ttttctgtgc ctgtactggc
     5581 cagtcagatt ggtggaatcc ctgatgttat tcagaacaac cagaccggga cattgttacc
     5641 agcaggtaat aagcacgcat ggatgtgcgc cctgaatgat ttttttaatg aacctgggcg
     5701 tttttatcag atggctcgcc aggcaaaaca ggatatagaa gagcggtttg atattaataa
     5761 aactgcgtta aaaatactca cattagcgaa gcacaagtaa caatatgttt tctgtaagta
     5821 acttatcatt tatcggtttc cttaaaagga ttgttttttc ctcagattca ctcccgggga
     5881 agtgggaaca cagaaaattc cggttcatgt acattttgcg atgtgctata aatccggttg
     5941 ccagtattcg atattattac gaactgcgtt ccttgcagtg cattgaggat attctggcaa
     6001 tacaacccac gttgccagca agaattcatc gaccttattt gcataaaggg gggcgagcct
     6061 ggtcccgggg gcaatatatt cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat
     6121 attctgaatt tttgtttcca caaaaatcgg tatcattagt ccaattcatc gggaaagatg
     6181 gagaggattt tgatatccag tgttctccca gtggttttga ccgggaagga gagttgatgt
     6241 tgtcattgtt tttcaatcaa atagtgattg cccggttaac attttcagtc attctgactc
     6301 aaaatggaca caccgctttt attggcggat tacagggagc ccccaaaaat accggacctg
     6361 atgttattcg gtgcgcaacc cgggcctgtt atgggctttt cccaaaacgg attatttttg
     6421 aagcattttg tgcgttgatg aaagcttgta atgtctctga gtgtctggca gtcagtgagc
     6481 acagtcatgt tttccggcaa ttgagatact ggtatcagaa acgcaaaacc tttgttgctg
     6541 tctacagtga tttctgggag tctgtagcag ggaaaacctg tggtgactgg tacaaattac
     6601 caacacaggt ggttcgaaag ccgctaagca atatagctag taaaaaacgc tctgagtatc
     6661 gaaaacgata cgctttgctg gattatatcc atgaaactgc tatccgctct ttggatgcat
     6721 atcctgtaaa ctcagaacat caggacttaa attaaggttt gatgtattct ctgaataaaa
     6781 tattaatgat gattttggta ggggcattcg cactaaataa tgaaaaaata caaatccgag
     6841 tttattcctg aatttaagaa aaattatctt tcccctgttt actggtttac atggttcgtt
     6901 ttgggaatga ttgcaggtat ttcaatgttt cccccttcat tcagagatcc tgtcttggcc
     6961 aaaatagggc gttgggtggg cagattgagc agaaaagctc gtcgcagggc gacgattaat
     7021 ttatcgcttt gtttcccgga aaagagtgat acagaacggg aaataattgt cgacaatatg
     7081 tttgccacag cattgcaatc catagtgatg atggcagaac tggcaattcg tggtccggaa
     7141 aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc ttgaggaaat ccggcacaat
     7201 aacagaaatg tgatttttct ggttccccat ggctggagcg tggatattcc tgcaatgttg
     7261 ctggcagctc agggggaaaa aatggccgcc atgtttcatc agcaacgaaa tccagtgatt
     7321 gattatgtct ggaattctgt acggcgtaaa ttcggggggc gcttacattc ccgggaggat
     7381 gggataaaac catttattca gtcagtacgc cagggatact gggggtatta ccttccagat
     7441 caggatcatg gtcctgaata cagtgaattt gctgattttt ttgcgaccta taaagcgaca
     7501 ttaccaatta ttggacgtct gatgaacatc agtcaggcta tgattatacc gcttttcccg
     7561 gtttatgatg aaaaaaaaca tttcctgact attgaagttc ggccaccaat ggatgcatgc
     7621 attgccagcg cggacaataa aatgattgcc cgacaaatga acaaaacagt ggaaattttg
     7681 gtggggtcac atccggaaca gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca
     7741 aacgaagcgg acccgtaccc ttgaaattat aaagtacagg tatttccact agttgtttct
     7801 tacaggttac caatcgaaac acatccccct tccgtgtgtt gcccgtttgg cacggaggac
     7861 tgtgtagatg cagttcgtta aatttaattt atg
//
LOCUS       DADLTI010000170         7859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLTI010000170 DADLTI010000000
VERSION     DADLTI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.172"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..165
                     /locus_tag="JN755_003518"
     CDS             <1..165
                     /locus_tag="JN755_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382180.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            348..2528
                     /locus_tag="JN755_003519"
     CDS             348..2528
                     /locus_tag="JN755_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7382181.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2669..3952)
                     /locus_tag="JN755_003520"
     CDS             complement(2669..3952)
                     /locus_tag="JN755_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7382182.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4087..5157)
                     /locus_tag="JN755_003521"
     CDS             complement(4087..5157)
                     /locus_tag="JN755_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7382183.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5135..5353)
                     /locus_tag="JN755_003522"
     CDS             complement(5135..5353)
                     /locus_tag="JN755_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7382184.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5438..6061)
                     /locus_tag="JN755_003523"
     CDS             complement(5438..6061)
                     /locus_tag="JN755_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7382185.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6383..7090)
                     /locus_tag="JN755_003524"
     CDS             complement(6383..7090)
                     /locus_tag="JN755_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7382186.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7038..7472)
                     /locus_tag="JN755_003525"
     CDS             complement(7038..7472)
                     /locus_tag="JN755_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7382187.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7545..>7859)
                     /locus_tag="JN755_003526"
     CDS             complement(7545..>7859)
                     /locus_tag="JN755_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7382188.1"
                     /translation="VQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIAL
                     TVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVG
                     DA"
BASE COUNT         1812 a         1957 c         1928 g         2162 t
ORIGIN      
        1 actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa cgatattaaa
       61 tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca tatcaccctc
      121 ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata ctcgtcatac
      181 ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc
      241 ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt atatccattt
      301 attatctttc tgcgcacttc acggtgcgca gatatctgga gcatttgatg atcaagttat
      361 ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa catttcaccg
      421 gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt ctctcttctc
      481 ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta gcctctcacg
      541 atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa cgtttcccgc
      601 tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc accattaacg
      661 gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt tttgtgcctc
      721 cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctggcggg ggcaaaatga
      781 tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca gtcggtgagg
      841 gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac tatccgggcg
      901 tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag gatgtggcgc
      961 tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc atggagttcg
     1021 ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac gtgatgacca
     1081 cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat aactggctgg
     1141 cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg gcgtactacg
     1201 atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg ccgttccacc
     1261 cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt ctgcgtgaga
     1321 ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg ctggataaag
     1381 tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct ggcggtaact
     1441 acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc aatgacacct
     1501 tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctagcc aaaaaaggtg
     1561 tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt ggcccatgct
     1621 ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc acgcgtaact
     1681 tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg gcgctgatgg
     1741 acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct gccagcgaac
     1801 ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat aaaaaccgtg
     1861 tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga ccgaacatta
     1921 aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg tgctcgaaga
     1981 tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc tcttcttatc
     2041 gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg tttgcgcgca
     2101 ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt ggcagcatgg
     2161 tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg
     2221 tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac cgttctaacg
     2281 tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt gaagtggggg
     2341 attacattta catccctggc attaaagcgg cgctggataa tccgggtacg acgtttaaag
     2401 gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg gaaagcctga
     2461 cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccatc
     2521 agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc ggatgcgatg
     2581 ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata aggcgtttac
     2641 gccgcatccg gcaaatagtt aatagctctt acttcttcgc ctctgcaacc actttgctgc
     2701 ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct
     2761 gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga ttagagatca
     2821 ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta tcacggatca
     2881 ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc gccacgccat
     2941 caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg ttagacagcg
     3001 tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg cggaattcgg
     3061 tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc
     3121 tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc acaccgctgt
     3181 tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc tgtacctggt
     3241 caccgtcagt acgcagtgct accgccggat ggttacctgc atctacacta tcgcccagcg
     3301 tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag aagaccgcag
     3361 aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac atataccacg
     3421 ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc
     3481 tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt
     3541 acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac tcaccaggca
     3601 tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct accgccgcct
     3661 gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg
     3721 caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg gatgcaaaat
     3781 agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga gaagaggtac
     3841 caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag gcggtcagcg
     3901 tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct
     3961 ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taagttggca
     4021 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcattatc agtcaaaata
     4081 aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac gatactgtga
     4141 tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc ttatctgctt
     4201 ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat ccccttcgaa
     4261 ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc cggatgaaag
     4321 cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt tgcatttatc
     4381 aagtgtttcc ttcattgata tcccgagagc atcaacatgc aatgttgtag ggatggcaat
     4441 ttttacgcct gttttgcttt gctcgacata aagatatcca tctacgatat cagaccactt
     4501 catttcgcat aaatcaccaa ctcgttgccc ggtaacaaca gccagttcca ttgcaagtct
     4561 gagccaacat ggtgatgatt ctgctgcttg ataaattttc aggtattcgt cagccgtaag
     4621 tcttgatctc cttacctctg attttgctgc gcgagtggca gcgaccgggt ttgttgttat
     4681 atggccttca gctattgcct ctcggaatgc atcgctcagt gttgatctga ttaacttggc
     4741 tgatgccgcc ttgccctcgt ctatgtatcc attgagcatt gccgcaattt cttttgtggt
     4801 gatgtcttca agtggagcat caggcagccc cctccttatt gctttaattt tgctcatgta
     4861 atttatgagt gtcttctgct tgattcctct gctggcgagg attttttcgt agcgatcaag
     4921 ccatgaatgt aacgtaacag aattatcact gttgattctc gctgtcagag gcttgtgttt
     4981 gtgtcctgaa aataactcaa tgttggcctg tatagcttca gtgattgcga ttcgcctgtc
     5041 tcggcctaat ccaaactctt tacccgtcct tgggtccctg tagcagtaat atccattgtt
     5101 tcttatataa aggttagggg gtaaatcccg gcgctcatga cttcgccttc ttcccatttc
     5161 tgatcctctt caaaaggcta cctgttactg gtcgatttaa gtcaaccttt accgctgatt
     5221 cgtggaacaa atactctctt ccatccttaa ccggaggagg gaatatcctg cactcgcgta
     5281 cccatcgacg aactgtttca aggctccttg ggcgtcgctg gcgtgcgttc cactcctgaa
     5341 gtgtcaagta catcgcaaag tctccgcaat tactcgcaag ggagcgagta ttggtttatt
     5401 ttctccaaca aaaaaggagc cgaagctcct ttgatgatta aaattcgaat tgtctcgccc
     5461 gaaggctttt cagcattggt tttgcccgtt cgaaaagggc gcttgcttgg tcaagtcgtg
     5521 tcgcctccct tagcagtaca tctctgtttt tcgtcaccgt gtaaaaagtt tcaaacgcga
     5581 tgtcatacag cttgctcgtg tatgatgagt tcagttcctt caatatcggg tacagacgtt
     5641 tgcagatgtc ctgcgcattc tccatctgta cctgcatata gcaaaggagg atgatttctt
     5701 cgtctgtgaa ttgctgcgct ggttgcatgc tgcgaagttt cttttcgcac tcgatgaagt
     5761 atcggcgtat ctggcggcct ttttcgttac gctcgaccat cgccgtttct ttggctgtat
     5821 cgagggtgag gtgatagtct ttgctacggc gatcgcctcc acgtcctttg atccccctgt
     5881 tttggtgaac caaaatgtaa tcgacatcaa cggcaaagcc gtattctgtt atgcgatttt
     5941 taatccatgt agagaagtca cgacgtactt ccaaaaattc gtgtaaatca cgggcattaa
     6001 cgagaagagt tgtttcgttg gatatagtgc cgttgaatac ggggatgagt tgactggtca
     6061 tttttatgtc ctttgcattt gttcagatag ccctgtgttc agcagggcgg tcaggtactt
     6121 gaacaccgtg caaagtcggc ccgtatcctt aacctttcgg ctgtttttcg gtatacgcgc
     6181 tacccgacca tatctgaaaa atggacataa aaaatccgca tgactgacgg gtgcggtttc
     6241 cgctttacaa ggtgtgttca gcaccatgaa gcggaatata gccccgttaa tgcggtgttg
     6301 tcaaatcatg taggccttat cttgctgtaa gccgcgccat tcgggctttt ccccacattt
     6361 ggggaaaact atcttgtcaa aatcatgcag cctttcgctc tccttcgatg agttcctcta
     6421 ttttgtgcac tccagcctcg ttgtaccgaa atgtttcaac ctgcttgtca ttgtgcgccg
     6481 atttgtcgat gaaccacttc ccgtactttt ctgttttgag catgtaggtg ttggctatgc
     6541 gtcctacctt gttggctgaa ataccgtagc gctggccgac ttccgtagca gagtagtagt
     6601 gctctgagat agctggcaga ggtacaacat cgcgaccaac aatcgggttg aacactccag
     6661 ctactgctgc ctgctttgat tctgaggaca gattcgggaa catattcacc agtcgatccg
     6721 ccacgttcaa agctatttcc atggctcgcg cacggtctag gctactttct ggttgcggta
     6781 ctggttgagg atggttggca accttttcgc gctgattgaa gtagccgtcc tccagttttt
     6841 cgaacacgtc ccatgcctga tccgtttcga gcatcttcgc gtgacgggcg gctccgcgct
     6901 ctgtccagag gatgaggtgg cgtgcatttg gggcactttt cacaaagtag tttaaagcta
     6961 ctttgtgttt tagcgcgcgc agctcgtcac caacgacctt gtagaagtgt accccctcaa
     7021 caaagcgcga cttgttttca tgatggtttt gccggatgcg cacaacatcc gttccgtaca
     7081 actgcgccaa tgtttggtgg gtagttgttt ttaaagtcag ctgctaacac atagcattgt
     7141 cgctctccca tttctggcat tcgctcacta cagcttatcc aggcatccgg agttaccggc
     7201 actggctggg cggtataaag cggtgttata tctgcccgaa aattacatgc tttatgcagc
     7261 cgcacccacc gttcgacttc tgctttgtca gaatacatac cagtgaacgt gttatattca
     7321 cggtcaattt gcgtgaaggt taccttccac gccaccggct ctgctgtcag cgatgccaga
     7381 gcaatttcat aagcacggcg ttcaacattg tctcgcacat ccaaactgct gatacgctct
     7441 ttgatttctt taatcagttc tttgtcgttc acttatgcct ccgattcccg gatgtcaccc
     7501 aaatcaacgc tacaatgcca accagtgcaa cgagcgcaaa actatcaagc atctccaacc
     7561 tctttaacgg taactgtctg tgcctccagt tctgctatgc gcttttctga ggcttcaagt
     7621 aacgcctgct tatcgcgtag cgcttcttcc agttcagcaa catggcattc actatcaata
     7681 aggttgttct ctgctgcttc cagctcaaca cgcaacttcc ctaccgttag cgcaatttcc
     7741 tcgttctcct gatcgcggct tttgatgtat tgcaggtttc tttcccgttc atccagtagt
     7801 gccagcgcaa catttggatt aaaagcagca ataaattcag cgtttgcata agcctgaac
//
LOCUS       DADLTI010000171         7835 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLTI010000171 DADLTI010000000
VERSION     DADLTI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.173"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(196..1449)
                     /locus_tag="JN755_003527"
                     /pseudo
     CDS             complement(196..1449)
                     /locus_tag="JN755_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1556..2449
                     /locus_tag="JN755_003528"
     CDS             1556..2449
                     /locus_tag="JN755_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7382189.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2584..3804
                     /gene="mlc"
                     /locus_tag="JN755_003529"
                     /gene_synonym="dgsA"
     CDS             2584..3804
                     /gene="mlc"
                     /locus_tag="JN755_003529"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7382190.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3929..4624
                     /gene="bioD"
                     /locus_tag="JN755_003530"
     CDS             3929..4624
                     /gene="bioD"
                     /locus_tag="JN755_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7382191.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4577..5869)
                     /gene="clcB"
                     /locus_tag="JN755_003531"
     CDS             complement(4577..5869)
                     /gene="clcB"
                     /locus_tag="JN755_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7382192.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6028..6642)
                     /gene="dmsD"
                     /locus_tag="JN755_003532"
     CDS             complement(6028..6642)
                     /gene="dmsD"
                     /locus_tag="JN755_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7382193.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6685..7539)
                     /locus_tag="JN755_003533"
     CDS             complement(6685..7539)
                     /locus_tag="JN755_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7382194.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7541..>7835)
                     /locus_tag="JN755_003534"
     CDS             complement(7541..>7835)
                     /locus_tag="JN755_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382195.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
BASE COUNT         2073 a         1998 c         2053 g         1711 t
ORIGIN      
        1 ggtagaataa ctgcgcatca gtaataaatg acacacagca aaatgaatcc gtttatttgg
       61 gtacttataa tcctgatgac gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt
      121 ttaggatggc tattaacgtt ggtttgagca ggcaataagt ccggacgggt atttaccgca
      181 gtccggactt atttttcagg cgtgcagacg acgatgcaaa cgcgccccga ccagcagagc
      241 aatgaccagc atcagagcaa taaatgcgcc gacgccgttc cagccatagt tatgccagaa
      301 aacaccaccc agcgtcccgg caatactcga ccccagatag taactgaaca gatacagcga
      361 ggaggcctgg cctttagcgc gttttgcgcg ggggccgatc cagctgctgg ctactgagtg
      421 ggctgcgaag aatccggctg agaagagtaa cattccggca aagatcagcc acagcgagct
      481 aaataaggtc atcagtaaac caaacggcat aactcccgtc gaaaacaaca tcaccggacc
      541 gcgcccatag cgggtggtca tggttccggc tttgggtgag ctccatgtac cggtcaaata
      601 agcccgcgat aataagccaa ccacggcctg actgacatgc tagggggaaa gcatcaaccg
      661 atagccgatg taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa
      721 caataacggt aatccccggt cacgccagtg cagacgaaag ttgataaata acgtcttagg
      781 gcgcagcgaa gtcgggcaaa aatggcgtga ttcagggagg attttccaga acatcaacgc
      841 cgaggccagc gcgaaacaac cgattgccgc cagagcaatt cgccagttga aaaagtccgt
      901 gaagacacca ctaattaagc gtccgctcat gccgccaatt gagttgccgc tgatatacaa
      961 ccccatcaaa aaggccacga aactgggggg gatttcctcg ctaagataag tcatgccaac
     1021 agctgccacg ccacttaacg aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca
     1081 gctggtcatc attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat
     1141 cactggtttg cgaccaatgg catcggatag cgggccagta aatagcaaac cgatagccaa
     1201 catcgccgtg gaaatggaca gtgaaatact actgttcgcg ggcgttaagc caaactcctg
     1261 cgaaagcacc ggaaggatag gctgcacaca atagagaagt gcaaaggttg ccagtccggc
     1321 agagaacagc gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt
     1381 tggctgagaa atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt
     1441 acggctcact tgaaatcctt gctaaatatg cctgtggatc aggcttatac atagggtagg
     1501 aaaatcgaat tgttctgtct aatatattaa taatctcaaa taagatgttt taaatatgaa
     1561 tattgaactt cgtcatctgc gttactttgt tgctgttgcg gaagagctgc attttgggcg
     1621 cgcggctgcc cgcctgaata tttcgcaacc gccgctaagt cagcagattc aggcactgga
     1681 gcaacaaatc ggtgcccgac tgctggcacg aactaatcgc agtgtattgc tgacggcagc
     1741 aggaaaacag tttcttgcag atagtcggca aatcctgtct atggtggatg acgctgccgc
     1801 tcgtgctgaa agactgcatc agggtgaagc gggggagttg cgtattggtt ttacttcgtc
     1861 cgctcctttt attcgggcgg tgtccgatac gttatcgctg tttcgccgtg attatcctga
     1921 tgtccattta caaacccgcg aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg
     1981 aacgctggat atgggattgc tgcgtaacac agcgttaccg gagacgcttg aacacgcagt
     2041 catcgtccat gaaccgctta tggcgatgat cccgcacgat catcccctgg caaataatcc
     2101 gagtgtaaca ctggctgaac tggcgaaaga accctttgtc ttttttgatc cgcacgtcgg
     2161 gactgggctg tatgacgata ttctcggact gatgcgacgt taccatttga cgcccgtcat
     2221 cactcaggag gtgggcgagg caatgaccat catcggtctg gtttccgccg gtctgggtgt
     2281 ttcaattttg cctgcgtcat ttaaacgtgt tcagctcaat gaaatgcgct gggtgccgat
     2341 tgctgaagag gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag
     2401 tccagctgcg cgtaactttc gtattcatct gctgaatgct ctcaggtgag ggaaatttca
     2461 gcgaaaaagc ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt tgacgacacg
     2521 tattgaagtg cttcaccata gcctacagat tatttcggag cgcgaaaata tagggagtat
     2581 gcggtggttg ctgaaaacca gcctgggcac attgatcaaa taaagcagac caacgcgggc
     2641 gcggtttatc gcctgattga tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg
     2701 gcgcagctgg ctcctgccag tatcactaaa attgtccgtg agatgctcga agcacacctg
     2761 gtgcgagagc tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt ggggctggtg
     2821 gttgaaactg aagcctggca ctatctttct ctgcgcatta gtcgcgggga gattttcctt
     2881 gctctgcgcg atctgagcag caaactggtg gtggaagagt cgcaggaact ggcgttaaaa
     2941 gatgacttgc cattgctgga tcgtatcatt tcccatatcg atcagttttt tatccgccac
     3001 cagaaaaaac ttgagcgtct aacttcgatt gccataaccc tgccgggaat tattgatacg
     3061 gaaaatggta ttgtacatcg catgccgttc tacgaggatg taaaagagat gccgctcggc
     3121 gaggcgctgg agcagcatac cggcgtaccg gtttatattc agcatgatat cagcgcatgg
     3181 acgatggcag aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt
     3241 atcgatcaca acgtgggggt gggcgtcatt accgatggtc atctgctaca cgcaggcagc
     3301 agtagtctcg tggaaatagg ccacacacag gtcgacccat atggtaaacg ctgttattgc
     3361 gggaaccacg gctgcctcga aaccatcgcc agtgtggaca gtattcttga gctggcacag
     3421 ctgcgtctca atcaatccat gagctcgatg ttacatgggc aaccgttaac cgtggactca
     3481 ttgtgtcagg cggcattgcg cggcgatcta ctggcaaaag acatcattat cggggtgggc
     3541 gcgcatgtag ggcgcattct tgccatcatg gtgaatttat ttaacccaca aaaaatactg
     3601 attggctcac cgttaagtaa agcggcagat atcctcttcc cggtcatctc agacagcatc
     3661 cgtcagcagg cccttcctgc gtatagtcag cacattagcg ttgagagtac tcagttttct
     3721 aaccagggca cgatggcagg cgctgcactg gtaaaagacg cgatgtataa cggttctttg
     3781 ttgattcgtc tgttgcaggg ttaacatttt ttaactgttc taccaaaatt tgcgctatct
     3841 caatttgggc caggaaagca taacttagac tttcaaggtt aattattttc ctagtttata
     3901 tttgtgaagc ataacggtgg agttagtgat gctgaagcgt ttctttatta ccggtacaga
     3961 cacttctgta gggaaaacgg tggtttcccg cgcattgcta caagcgttag cctcccaggg
     4021 aaaaacggtt gcgggatata aacccgtagc gaaggggagc aaagagacac ccgaagggct
     4081 gcgtaataaa gatgccttgg tgttgcagag tgtttcaacc atcgaactgc cttatgaagc
     4141 agttaatcct atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa
     4201 ttacaccctc atttcaaacg gcctggcaaa cctgaccgat aaagtcgatc atgtcgtggt
     4261 agaagggact ggcggctggc gcagtctgat gaatgatttg cgtccactct ctgaatgggt
     4321 agtgcaggaa caactgccgg tgttgatggt tgtcggtatt caggaaggtt gcattaacca
     4381 tgcactgcta acagctcagg cgatcgccaa cgacgggctg ccgctcattg gctgggtggc
     4441 taaccgaatc aacccaggac tggcgcatta tgcggaaatc attgatgtgc tgggtaaaaa
     4501 acttccggca ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac agcgtgaact
     4561 ggggcaatac atccgcttag ctatgctgcg cagtgtgctg gcggtagata gagtcacggt
     4621 gtaacgtccg cgaaattacc gacgcaatta cgcaggcaat caataaaccg gggagtagct
     4681 gatactcccc ggtcatttca catatcatca acgtcgacat aatcggcgcg tgcgtggttg
     4741 ccgccaacag tgtcgccatc ccggtcaatc cgagtaaaag cgtaatttct tcgccatcag
     4801 ggaaccataa tcccaggcta cgaccataca acatgccaat ggcaagaccg ataaatagcg
     4861 tcggtgtaaa gacaccaccg ggtgcgccgg aaccgctact cgccagcacg gcacacagtt
     4921 tacaaaggaa gatcccggca atgatcatta acagtggtgg ggcggttaaa aaggattgta
     4981 cggtgctata gccgttgccc cacactgcag gtgtgaacag ggaaagcaga cccacgatca
     5041 acccgcctag tgccagttgc cagggcggcg caagtttgag actcacaaat ccacgatgac
     5101 aggcgttcat taacgttaac aacagtggtc cgcacagacc tgccagcaca cctgtactga
     5161 taatcagcgc atagtcacga gcctgaaccg tcactgagag ttgtacgttg tagagtaacg
     5221 cgtcgctatg attaatcaga ttgctaacca gcaatgccac gacggcggaa ataatcaccg
     5281 ggccgagaga ggccaacatc atagtgccaa acagcacttc ggctataaat aaactgccag
     5341 caagcggggc acgataggcc gcagccattc ccgccgcggc cccacaggcg atccataatt
     5401 tccattcctg gcgtggcgta aaacgttggg caaaacagga ggcggcaagg gcagctaaaa
     5461 gaatcatcgc accttcgcga ccaattgcac tgccgctggt tactaccagc agcgaggcaa
     5521 gcgatttaac caggcttgct gcgtaatcga actgtccatc ggtttgcaac gcttccatgt
     5581 aatcggtcgg cgcatgaggg cgttgttggg taaatttctg ccagcccatc agcaacaaac
     5641 ccgccgccag tccgccgagc gccggagtta gcagccgtcg ccagggggaa aggtttgtcg
     5701 ctgcattgac cagactgccg gagtcattat tgaggaacaa ccactccagt agcagcatcg
     5761 catgacgaaa cccggcaacg gcaaagacag cgagaatacc gacgactgtt gcgataagca
     5821 gacggcggaa catagtgcgc agatccggat agatgtgtag atgatgcatg agcagaaatg
     5881 ggcaatgaaa cacgggcgac tattttgcgg caaaagaggc caaccgcaaa agcagtgcgt
     5941 gctttaagag ataaaaaaac gtgacattgt aacgtattat tgccgaatcg cgcgtaatcg
     6001 tcaccatccg gcaatattac ggtgatccta tcgaaagagt ggtttaaccg cgacaggaat
     6061 caacagttgt gattgccatt gcgccagcgt taagcgagcc agttcaccca gtgcacggta
     6121 gaaagggtgt tctgcttttt cgataaaaac atcaagaaaa cgtgttgacc acggaaaaag
     6181 gtgccatgcc agcaattctt cgcattctgt ctggcgacca ttctccgcca accacgcagc
     6241 catcaacagc agtgaaccaa aatgatcttc cggttcgttt tgcttcattt caaactgaat
     6301 gcctttctcg cgcatccact gacgaagtgc caatgttgaa tcgccaaaca gcacagattc
     6361 gcgatccagc cagaccgacc cccacggcgg agacggcagt gcccacgggc cgacaaacaa
     6421 acgctgccag gcctgggcgt gagtctcttc acactgtgtc tgaaaagccg tcaccagcgg
     6481 cgctaatgac gcttctggta gaggccactg agtttcccag ccgtcactgg tgagtaccgc
     6541 aaccagaggt gcggcttccg cactctctgg agcgtaataa aacagcgctc ccaacacgcg
     6601 cgccgcgaca gaaaaattat cttgctgtga aaaatgggtc attcctaaca tcctgaaatg
     6661 tgcgggttgc cccgcaccaa tttgttaacc tgcaatagcc attcctactg tcatgtgcag
     6721 gccataaaag agcacacgcc cgatcatctc accaacaagt atgaggatta gccccagaac
     6781 aagtccggca acgtgaggtt cacggcgacg aatgagcgga caaagccagc atccaagacc
     6841 tgcgcataac aataccacac gccagacctg taaggaggcg taatctggta ccagtgcgct
     6901 ggcttgttgc acggatgagt gaatagacgc cagagacaaa ccttgcagca caatcacctc
     6961 ggcacaagcg atcagcgcca gaacgctaat gatggcaaat ggtgtggtat taaatgtaac
     7021 gcgggctgcc cgcagaattg cggcagcaag tattggaccg ctcaacaata ccgtcaggaa
     7081 gaaagccagc gtagtgtaac cgttatgcca ggttggcacg gtgtcgattt gatacacgca
     7141 ggtcatcatc cagacgaaaa tgacgccgag cgccatgctg acaagtaacc agagtttccc
     7201 caacgcttgc ggcattttac cgatgaccgc caccagccac catagtccgc caacggcaaa
     7261 aaatatggaa ccggcagcaa tctcgttact cagaccagaa gctccgattc gattcagcga
     7321 gttaaacgct cgcagtggcg atcccaggtg catgacagag gcaataaacc cgatgcccat
     7381 caacagccag aggaaaaaca tgccacgaac aatgtgctga cggtctgcgt catttttggc
     7441 ggcgaaccag ccaattccgc taacaatcag cgcgcctact acgcattgac ccaatacggt
     7501 aaagataacc agtggccatt catgccatcc atttcccatt ttacacttcc tccggattag
     7561 ccagataacc agtggtatcg ccggtcgggc ggctgttggc gttaggtttg ataacgatat
     7621 tgggttttgt gaaatgcgcg cgcggcagtg gcgcgacggc agccagagta ccgtgcttct
     7681 ggcgcagctc ttcaattgga ccgaactcaa gtgcacgcag cgggcaggat tcgacgcata
     7741 tgggttgttt cccctcggcg acgcgcgaat aacaaccatc gcacttcgtc atgtgccctt
     7801 tttcagcatt gtactgtggt gcgccgtacg gacaa
//
LOCUS       DADLTI010000172         7795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLTI010000172 DADLTI010000000
VERSION     DADLTI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.174"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(337..627)
                     /locus_tag="JN755_003535"
     CDS             complement(337..627)
                     /locus_tag="JN755_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382196.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JN755_003536"
     tRNA            complement(1227..1302)
                     /locus_tag="JN755_003536"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JN755_003537"
     CDS             complement(1408..2115)
                     /locus_tag="JN755_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7382197.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JN755_003538"
     CDS             2513..4648
                     /locus_tag="JN755_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7382198.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JN755_003539"
     CDS             complement(4697..5953)
                     /locus_tag="JN755_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7382199.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JN755_003540"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JN755_003540"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7382200.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JN755_003541"
     CDS             complement(7299..7574)
                     /locus_tag="JN755_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7382201.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..>7795)
                     /locus_tag="JN755_003542"
     CDS             complement(7602..>7795)
                     /locus_tag="JN755_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7382202.1"
                     /translation="TAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSV
                     GLAAPVERLLQQLRTGAPV"
BASE COUNT         1908 a         1879 c         2007 g         2001 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtca
//
LOCUS       DADLTI010000173         7637 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLTI010000173 DADLTI010000000
VERSION     DADLTI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.175"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..423
                     /locus_tag="JN755_003543"
     CDS             <1..423
                     /locus_tag="JN755_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382203.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            595..702
                     /locus_tag="JN755_003544"
     CDS             595..702
                     /locus_tag="JN755_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382204.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(755..1513)
                     /locus_tag="JN755_003545"
     CDS             complement(755..1513)
                     /locus_tag="JN755_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7382205.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1799..2728
                     /gene="pfkB"
                     /locus_tag="JN755_003546"
     CDS             1799..2728
                     /gene="pfkB"
                     /locus_tag="JN755_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7382206.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2829..3119
                     /gene="ghoS"
                     /locus_tag="JN755_003547"
     CDS             2829..3119
                     /gene="ghoS"
                     /locus_tag="JN755_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7382207.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3225..4085
                     /locus_tag="JN755_003548"
     CDS             3225..4085
                     /locus_tag="JN755_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7382208.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4126..4662)
                     /locus_tag="JN755_003549"
     CDS             complement(4126..4662)
                     /locus_tag="JN755_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382209.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4809..5476
                     /gene="hxpB"
                     /locus_tag="JN755_003550"
                     /pseudo
     CDS             4809..5476
                     /gene="hxpB"
                     /locus_tag="JN755_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5639..6229
                     /locus_tag="JN755_003551"
     CDS             5639..6229
                     /locus_tag="JN755_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7382210.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6362..7637
                     /locus_tag="JN755_003552"
                     /pseudo
     CDS             6362..7637
                     /locus_tag="JN755_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
BASE COUNT         1949 a         1847 c         1856 g         1985 t
ORIGIN      
        1 catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt gtgtgtcaca
       61 cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa agctaacatc
      121 atggatttat taaaaggagc tacagcacag ggaccccctg cattatacat cgccatgagc
      181 aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa
      241 cattctttta gtcaacatca gttatttaca ctattggccg ctaaaaatca tgacaacatg
      301 tcagctgttc atatagccat tcatcataag cattataaaa ctgtagaaac atattatgct
      361 gctataaatg tcatcagcca aagcctgagt tttagtgctg atgaaataaa gacgtattta
      421 taaccagcta tttacgatat atatcgatac ataattttat ttttttcata aaacgcagaa
      481 acagtgacca tgtgcacaac gccacccgac ggctgtaata acaaaaaatc ccttgatgcc
      541 tgtccctttt gttacactcc gttatcacgc acaagagata tgcaggacac tggtatgccg
      601 actaaacgct ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc
      661 gctatgttgt tgctacgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac
      721 agccagaccg cataacacgg tccggcagat aattttacat gctgtagccc agcgataagg
      781 ttgtccgacg gtcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt
      841 tgtaagccac cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt
      901 tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag
      961 tcaactgcca tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt
     1021 cggtatattt gtcgtaacgc acacctggac cgaattcaaa gcggaaactg tgtaccgggc
     1081 cattcaaaaa ctgacgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat
     1141 aacggtctgt cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaagc
     1201 ggctacgccc gcccgccgca tatttttctg aagaacgctc atcattagaa gaagtattac
     1261 tggcgttccc ccacagcgac caggcagtgg tttgtccata ccaggtcatg gtggtgtcag
     1321 ccgtaagtga ggagcttttc gtattgcctg attgtgcaag atatcctgcg ttcagattac
     1381 cttcgaaagg ttttttagcg ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg
     1441 cagctgcatt cagtgacgca aacatgcccc ccgccaacat aacgatggca ggaactgtct
     1501 tcaaaagctt catttatcaa gagtccgtac aacaaaaaag agaccatcac ggtcccggaa
     1561 actttcttaa ggatcaaaga ttagcgtccc tggaaaggta acgaattata aaaaggcgcg
     1621 aataacttag caatgtattc ttatttcatt ttttgaataa gcatgtggcg aaaacagatt
     1681 tttatttata tatatttatc tgcaaaattt taaataaagc tctaataaat catattgtta
     1741 atttcttcac tttccgctga ttcggtgcca gactgaaatc agcctatagg aggaaatgat
     1801 ggtacgtatc tatacgttga cacttgcgcc ctctctcgat agcgcaacaa ttaccccgca
     1861 aatttatccc gaaggaaaac tgcgctgtac cgcaccggtg ttcgaacccg ggggcggcgg
     1921 catcaacgtc gcccgcgcca ttgcccatct tggaggcagt gccacagcga tcttcccggc
     1981 gggtggcgcg accggcgaac acctggtttc actgttggcg gatgaaaatg tccccgtcgc
     2041 tactgtagaa gccaaagact ggacccggca gaatttacac gtacatgtgg aagcaagcgg
     2101 tgaacagtat cgttttgtta tgccaggcgc ggcattaaat gaagatgagt ttcgccagct
     2161 tgaagagcaa gttctggaaa ttgaatccgg ggccatcctg gtcataagcg gaagcctgcc
     2221 gccaggtgtg aagctggaaa aattaaccca actgatttcc gctgcgcaaa accaagggat
     2281 ccgctgcatc atcgacagtt ctggcgaagc gttaagtgca gcactggcga ttggtaacat
     2341 cgagttggtt aagcctaacc aaaaagaact cagtgcgctg gtgagtcgcg aactcaccca
     2401 gccagacgat gtccgcaaag ccgcgcagga aatcgttaat agcggcaagg ccaaacgggt
     2461 tgtcgtttcc cttggtccac aaggagcgct gggtgttgat agtgaaaact gcattcaggt
     2521 ggtgccacca ccggtgaaaa gccagagtac cgttggcgct ggtgacagca tggtcggcgc
     2581 gatgacactg aaactggcag aaaatgcctc tcttgaagag atggtccgtt ttggcgtagc
     2641 tgcggggagt gcagccacac tcaatcaggg aacacgtctg tgctcccatg acgatacgca
     2701 aaaaatttac gcttaccttt cccgctaaca aaaacattcc cccagcattg ggggaatcac
     2761 caccaacctg tcggcaacgc gtttctccga ctatgctcaa aagtcatgtg ataacaaagg
     2821 ggtgaactat ggccagtggc gatcttgtcc gttatgtcat aaccgtcatg ttgcatgagg
     2881 atacattgac tgaaattaac gagttgaata attacctgac tcgcgacggt tttttgctca
     2941 ccatgacgga tgatgaggga aatatccatg agctggggac taacactttt ggacttatca
     3001 gtacccaaag tgaagaagaa attagagaac tggtttcggg gcttacccaa agtgcaaccg
     3061 gcaaagatcc tgaaatcacc atcacgacct gggaggaatg gaatagcaac agaaaataaa
     3121 tggtttttgg gcaataatca gtctgtggtg tgcgttagct cgtgttttta caccgcattc
     3181 ttgcgctaac cttatgatct ggcagacaac atgggagaga catcatgtgg caggcaatca
     3241 gtcgtctttt gagcgagcag ttaggtgaag gcgaaatcga actgcgtaat gaactgcctg
     3301 gcggagaagt ccatgccgca tggcatttgc gctatgcagg acatgacttt ttcgtcaaat
     3361 gtgatgaaag ggaactgctt cccagtttta ccgccgaagc cgaccaactg gagttactgt
     3421 cgcgtagtaa aaccgtcacc gtgcctaagg tttgggcagt aggcgctgac cgtgactaca
     3481 gttttctggt gatggattat ctcccacctc gtccgctgga tgcgcatagc gcatttattc
     3541 ttggtcagca aattgcgcgt ttacatcaat ggagtgacca accacaattt ggcctcgatt
     3601 ttgataactc gctctccaca actccacagc ccaacacctg gcaacgtcgc tggtcaacgt
     3661 tttttgctga acaacggatt ggctggcagt tggaactggc agcagagaaa gggatcgctt
     3721 tcggcaatat cgacgccatc gtcgagcata ttcagcagcg tctggcctcg catcaaccgc
     3781 agccttctct gttgcacggc gatttatggt ccggcaactg tgcgctgggt ccggatggcc
     3841 cgtacatttt cgaccctgcc tgctactggg gtgaccgaga gtgcgacctg gcgatgttac
     3901 cgctgcatac tgaacagccg ccacaaatct atgacggcta tcagtcagta tccccgctac
     3961 ctgccgattt ccttgaacgt caaccggttt accaactcta cacgctgtta aatcgtgcaa
     4021 gattatttgg cggtcagcat ttggttattg ctcagcagtc attggataga ttattagcag
     4081 catgatatgg gttgaggata atggccgctc cgtgcggcct tttgattaaa taaacccaag
     4141 cagagaaaag aacacataac ctgcagcaat acaaataacc ggcaggatat atagtgagaa
     4201 aaactgcagg aaaatagtat ggtgcggaac aacaatacgg gactcaattt gttcacgcgt
     4261 tagcccctct tcccctttgg ctttttccag aatgagttga tcttcaacac cttcccgcaa
     4321 gaagcgcgcc tggcgactca tccgggcacc ggaatcctgc aacgccagtc cgacaaaaat
     4381 caggatgaaa atcacccaga acataacgtt cacaccacca ttaaaattag gggtcgggga
     4441 gttataccag aacagattca aaaaaggcgt attcgcctgc atcatatcga tcatgacatg
     4501 agcaaagtcg agcatgaccg cattaatgcc ttcctggttt tcctgccggg tattcataaa
     4561 tttcagcaaa gaaatcaatg tagagatcaa tgcaggtata aaaatcaccc aacccaaaat
     4621 ccttttcaaa acagcaatgc gtccagcttg ttgatacgtc atgagttctc cttgattaag
     4681 acgcgtcgtt tcacttagtt tacctgtaga tatctgtttt cgcccattct ttaaaggcga
     4741 tatgataggc gcttaatcat aagcacggct taatacctta cacataatgc tctaaaggag
     4801 aggttgtaat gtcaaccccg cgtcagattc ttgctgcaat ttttgatatg gatggattac
     4861 ttatcgactc agaaccttta tgggatcgag ccgaactgga tgtgatggca agcctggggg
     4921 tagatatctc ccgtcgtaac gagctgccgg acaccttagg tttacgcatc gatatggtgg
     4981 tcgatctttg tacgcccggc aaccgtggaa tgggccaagc cgtcaggaag tagtagaacg
     5041 ggttattgcc cgtgccattt cactggttga agagacacgt ccattattac caggcgtgcg
     5101 cgaagctgtt gcgttatgca aagaacaagg tttattggtg ggactggcct ccgcgtcacc
     5161 actacatatg ctggaaaaag tgttgaccat gtttgactta cgcgacagtt tcgatgccct
     5221 cgcctcggct gaaaaactgc cttacagcaa gccgcatccg caagtatatc tcgactgcgc
     5281 agcaaaactg ggcgttgacc cactgacctg cgtggcgctg gaagactcgg taaatggcat
     5341 gatcgcttct aaagcagcct gcatgcgttc catcgtcgtt cctgcgccag aagcgcaaaa
     5401 tgatccacgt tttgtattag caaacgtcaa actctcatcg ctgacagaac tcaccgcaaa
     5461 agaccttctc ggttaatgac caggggcagt gatcacgctg cccctggttc tttatctgaa
     5521 tttgcattca actgacggat taatcgtcaa tttaagagaa agagttacac cgtcaccact
     5581 tccgtgcact gtataaaaat cctatactgt acgtatcgac agtttagtga gttttatcat
     5641 gacggcggaa ggtcaccttc tcttttctat tgcttgtgcg gtatttgcca aaaatgccga
     5701 gctgacgccc gtgctggcac agggtgactg gtggcatatt gtcccttccg caatcctgac
     5761 gtgtttgtta ccggacatcg atcacccaaa gtcgtttctt gggcagcgat taaaatggat
     5821 atcaaaaccg atcgcccgcg cttttgggca tcgtggtttt acccacagtc tgctggcggt
     5881 atttgcgctg ctggcaacct tttaccttaa ggttccggaa ggctggttca ttccggctga
     5941 tgcgctacaa ggaatggtgc tgggttattt gagccacata cttgccgata tgctgacacc
     6001 cgccggtgtt cccctgctct ggccatgccg ctggcgtttc cgcttgccta tcctggttcc
     6061 ccaaaagggc aaccaactgg aacgttttat ctgcatggca ttatttgtct ggtcggtatg
     6121 gatgccccat tcattacccg agaacagcgc tgttcgttgg tcatcgcaaa tgatcaatac
     6181 cttgcagatc cagtttcatc ggcttattaa gcatcaggtt gaatactaaa aaggcaaaaa
     6241 tcacctttct ggaataagca attccatttg aatataagag ccagctcaca gttctgttaa
     6301 tcttgcgcca acactatgac tgctacgcag tgatagaaat aataagatca ggagaacggg
     6361 gatgaacttt ccattaattg cgaacatcgt ggtgttcgtt gtactgctgt ttgcgctggc
     6421 tcagacccgc cataaacagt ggagtctggc gaaaaaagtg ctggtgggtc tggtgatggg
     6481 tgtggttttt ggccttgccc tgcataccat ttatggttct gacagccagg tacttaaaga
     6541 ttctgtacag tggtttaaca tcgttggtaa cggcaatgtt caactgctgc aaatgatcgt
     6601 tatgccgtta gtcttcgcct ctattctgag cgcggttgcc cgtctgcata acgcatctca
     6661 gttaggcaaa atcagttttc tgaccatcgg tacgcttttg tttaccacgc tgattgcggc
     6721 gctggtcggt gtgctggtca ccacctgttt ggtttgacgg ctgaaggtct ggttcagggt
     6781 ggtgcagaaa ctgcacgtct gaacgccatt gaaagtaact atgttggtaa agtctctgat
     6841 ctgagcgttc cgcagctggt cttgtccttt atcccgaaaa acccgtttgc cgatcttacc
     6901 ggagccaatc cgacgtcaat tatcagcgtg gtaatttttg ccgcattcct cggcgtagct
     6961 gcattgaaac tgctgaagga tgatgcgccg aaaggtgaac gcgtcttaac cgctatcgat
     7021 accctacaaa gctgggtgat gaaactggtt cgccttgtca tgcagttgac cccttacggc
     7081 gttctggctc taatgaccaa agtggttgca ggttctaact tgcaagacat catcaaactg
     7141 ggaagtttcg ttgtcgcgtc ctacctcggt ctgctgatta tgtttgctgt gcatggcatt
     7201 ctgctgggca ttaatggcgt gagtccgctg aagtacttcc gtaaggtatg gcctgtgctg
     7261 acgtttgcct ttaccagccg ttccagtgct gcgtctatcc cactgaatgt ggaagcacaa
     7321 acgcgtcgtc tgggcgttcc tgaatccatc gccagtttcg ccgcctcttt cggtgcaacc
     7381 attggtcaga acggctgcgc cggtttgtat ccggcaatgc tggcggtgat ggttgcgcct
     7441 acggttggca ttaacccgct ggacccgatg tggattgcga cgctggtcgg tattgttacc
     7501 gttagttccg caggcgttgc cggtgtcggt ggtggtgcaa ctttcgccgc actgattgtg
     7561 ctgcctgcga tgggcctgcc agtaaccctg gtggcgctgt taatctccgt tgaaccgctt
     7621 atcgacatgg gccgtac
//
LOCUS       DADLTI010000174         7494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLTI010000174 DADLTI010000000
VERSION     DADLTI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.176"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..532)
                     /locus_tag="JN755_003553"
     CDS             complement(<1..532)
                     /locus_tag="JN755_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY7382211.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVG"
     gene            complement(717..1478)
                     /gene="cysZ"
                     /locus_tag="JN755_003554"
     CDS             complement(717..1478)
                     /gene="cysZ"
                     /locus_tag="JN755_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7382212.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            1708..2595
                     /gene="zipA"
                     /locus_tag="JN755_003555"
     CDS             1708..2595
                     /gene="zipA"
                     /locus_tag="JN755_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7382213.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            2666..4681
                     /gene="ligA"
                     /locus_tag="JN755_003556"
     CDS             2666..4681
                     /gene="ligA"
                     /locus_tag="JN755_003556"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7382214.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            4683..4901
                     /locus_tag="JN755_003557"
     CDS             4683..4901
                     /locus_tag="JN755_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382215.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(4898..5896)
                     /locus_tag="JN755_003558"
     CDS             complement(4898..5896)
                     /locus_tag="JN755_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7382216.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            5986..6912
                     /locus_tag="JN755_003559"
     CDS             5986..6912
                     /locus_tag="JN755_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7382217.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(6903..7244)
                     /locus_tag="JN755_003560"
     CDS             complement(6903..7244)
                     /locus_tag="JN755_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7382218.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(7364..7489)
                     /locus_tag="JN755_003561"
     ncRNA           complement(7364..7489)
                     /ncRNA_class="other"
                     /locus_tag="JN755_003561"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         1893 a         1827 c         2026 g         1748 t
ORIGIN      
        1 tcccaacgcc agcaataaat acatcaacct gaccgtcggt atcttcccag atctccggac
       61 cggtggtttt ttcgtgaatt tcagggtttg ccggattgct gaattgttgc agcagcaggt
      121 atttctctgg attgctggcg acaatttctt ctgctttttg gattgcgcct ttcatgcctt
      181 tagcaccttc cgtcagcacc aggtttgcac ctaacgcttt cagcagcttg cggcgttcaa
      241 tactcatggt ttctggcatg gtcagggtga gtttgtaacc gcgagcggca gctacatagg
      301 ccagtgcaat cccggtatta ccgctggtcg gttcaaccag ttcaacgcct ggtttcagca
      361 cgccgcgctt ttcggcatcc caaatcatgt tggcaccgat acggcactta acgctgaagc
      421 tggggttacg agattccacc ttcgccagaa tgcgtccgtt accgatgcga ttcaggcgaa
      481 ccagcggcgt gtgaccgata gtcagcgagt tatcttcaaa aatcttactc atggcctgtc
      541 cttaactgta tgaaattggg atacaacagg tagcataccc gctcagagaa tatgcggaag
      601 taaggattta gcatatctat atacagaagg gaaataatga catgcaagat ggaataaggg
      661 gcggcataag ccacccctgt ttcacacaaa cggtttacaa aataaccggt agattgttac
      721 cgccacatcg cgtgtttatc gcgatagcaa tcgacccaca tcgccgtcgc gccacaaacg
      781 gcaacgggca tgatgaacag attaagcagc gggatcatcg taaacaggct ggttaaagca
      841 ccaaactgca tattggtgat tttgcgtgtg cgcagggcgg tgcgcatctc tttaaacggc
      901 actttgtggt tatcgaaggg gtaatcgcaa tactggatgg ctaacatcca ggcgctaaac
      961 aggaaccaca gtaccggcgc gacggtttgc ccaataccag ggatgaagta aagaattagt
     1021 aatacaattg cgcgcggcag ataccagaca aatttttgcc attcgcgttt catgattcgc
     1081 ggcacatctt tcatgatacc gaaaatcccg gtatctggcg gtgtagcgcc agtcaatcgt
     1141 gcttccagtt gttcagccaa taaaccgtta aacggagcgg caatccagtt agcaatcgtg
     1201 gagaagaaat agccaaacac taacagcaca gagatgacag ccagaggcca caacagataa
     1261 ctcagccatt gtagccagtc cggaacgtaa ctcatgaaag tcgggatcca gacatcgagc
     1321 tgtgtaaaga gccaccagaa tgcgcccccc atcaacaaaa tattgaccag cagcggtaaa
     1381 ataacgaaac gccgaatccc aggttgcgag acgagcttcc agccttgcgc aaaatagtaa
     1441 aaaccgctgc gtggggcaga tgtgaatgat gaaaccataa tcaggatgag ctccttttga
     1501 ccaatcccag gaaaattctg cgtattttac cgggtaattg cgcaatggac agttaggata
     1561 tgttcgaaaa aacagcaaaa agcacgattt catctatctt tgtgctgtga aagttaatag
     1621 tgcacttgca cttgaggtaa tcggcaaata ctcttagtga gtaaatgttt gccgtggtgg
     1681 caaggtgtta gaacaacaga gaatataatg atgcaggatt tgcgtctgat attaatcatt
     1741 gttggcgcga tcgccataat cgctttactg gtacatggtt tctggaccag ccgtaaagaa
     1801 cgatcttcta tgttccgcga tcggccatta aaacgaatga agtcaaaacg tgacgacgat
     1861 tcttatgacg aggatgtcga agatgatgag ggcgttggtg aggttcgtgt tcaccgcgtg
     1921 aatcatgccc cggctaacgc tcaggagcat gaggctgctc gtccgtcgcc gcaacaccag
     1981 taccaaccgc cttatgcgtc tgcgcagccg cgtcaaccgg tccagcagcc agtttcgcca
     2041 caggtcgcgc cagcgccgca gccagtgcat tcagcaccgc aaccggcaca acaggctttc
     2101 cagcctgcag aacccgtagc ggcaccacag cctgagcctg tagcggaacc ggctccagtt
     2161 atggataaac cgaagcgcaa agaagcggtg attatcatga acgtcgcggc gcatcacggt
     2221 agcgagctaa acggtgaact gcttcttaac agcattcaac aagcgggctt catttttggc
     2281 gatatgaata tttaccatcg tcatcttagc ccggatggca gcggcccggc gttattcagc
     2341 ctggcgaata tggtgaaacc gggaaccttt gatcctgaaa tgaaggattt cactactccg
     2401 ggtgtcacta tctttatgca ggtaccgtct tacggtgacg agctgcagaa cttcaagctg
     2461 atgctgcaat ctgcgcagca tattgccgat gaagtgggcg gtgtcgtgct tgacgatcag
     2521 cgcagtatga tgactccgca gaaattgcgc gagtaccagg acatcatccg cgaagtcaaa
     2581 gacgccaacg cctgatacac ttaaggcaaa ttaactcctc ttcgaacccc cgcttgtcgg
     2641 gggtttttag cattgatggt gcgatatgga atcaatcgaa caacaactga cagaactgcg
     2701 aacgacgctt tgccatcatg aatatcttta tcatgtgatg gatgcgccgg aaattcccga
     2761 cgctgaatac gacaggctga tgcgcgaact gcgcgagctg gaaaccaaac atccagaact
     2821 gattacgcct gattcgccta cccaacgtgt aggtgctgcg ccgctggcag ctttcagcca
     2881 gatacgccat gaagtaccaa tgctgtcact ggataacgtt tttgatgaag aaagctttct
     2941 tgctttcaac aaacgtgtgc aggaccgtct gaaaaacaac gagaaagtca cctggtgctg
     3001 tgagctgaag ctggatggcc ttgccgtcag tattctgtat gaaaatggcg ttttagtcag
     3061 tgccgcgacc cgtggcgatg gcaccaccgg ggaagatatc acgtctaatg tgcgtactat
     3121 tcgcgccatt ccgctgaagc tgcacggaga gaatatcccg gcgcgtctgg aagtgcgtgg
     3181 tgaagtgttc ctgccgcagg cggggttcga aaagattaac gaagatgcgc gacgcacggg
     3241 cgggaaagtt tttgctaacc cacgtaatgc ggcagctggt tcactgcgtc agcttgatcc
     3301 gcgtattaca gcgaagcgac cgctcacttt tttctgctat ggcgttggtg ttctggaagg
     3361 gggcgagctg ccggatactc atcttggccg tttactgcaa tttaaaaagt gggggttgcc
     3421 ggtcagcgat cgggtaacgc tttgtgaatc ggcggaagaa gtgctggcgt tctatcacaa
     3481 agtggaagaa gaccgcccga cgctgggctt tgatatcgac ggcgtggtga ttaaggtcaa
     3541 ctcactggaa cagcaggagc agcttggctt tgtcgcgcgt gccccgcgct gggcggtagc
     3601 gtttaaattc ccggcgcagg agcagatgac ctttgtgcgt gacgtcgagt ttcaggttgg
     3661 gcgtactggc gcgattacgc ctgttgcgcg tctggaacct gtccatgttg ctggcgtgct
     3721 ggtgagtaac gcaaccttac acaatgcgga tgaaatcgaa cgtcttggtt tacgcattgg
     3781 cgataaagtg gtgattcgcc gcgctggcga cgtgatcccg caggtggtta acgtcgtgct
     3841 ttctgaacgc ccggaagata cccgtgaggt tgtattcccg acgcattgtc cggtatgtgg
     3901 ttctgacgtt gagcgtgtgg aaggtgaagc ggttgcccgc tgtaccggtg gcctgatttg
     3961 cggtgcgcag cgtaaagagt cgctgaaaca ctttgtttcc cgccgtgcga tggatgttga
     4021 cggaatgggc gacaaaatca tcgatcagct ggttgaaaaa gaatatgtcc atactccggc
     4081 agatctgttc aaactcaccg caggcaaact gaccggactg gagcgtatgg ggccaaaatt
     4141 ggcacaaaac gtggttaacg cgctggaaaa agcgaaagaa accacctttg ctcgcttcct
     4201 ctatgcactt ggcatccgtg aagtcggcga ggccaccgca gcaggtctgg cggcatattt
     4261 cggcacgctg gaagcgctgg aagccgcttc gattgaagag ctgcaaaagg tgcctgatgt
     4321 tggcattgtc gttgcatccc acgttcacaa cttctttgcc gaagaaagca accgcaatgt
     4381 catcagcgag ctgttggcgg aaggtgttca ctggcctgcg ccgatcgtta tcaacgcgga
     4441 agagattgac agcccgtttg ctggtaaaac cgtggtgctt acgggcagct taagccagat
     4501 gtcgcgtgat gacgctaaag ctcgactggt cgaactgggc gcgaaagtcg cgggcagcgt
     4561 gtcgaagaaa accgatctgg tgatagcggg tgaagctgca ggatctaaac tggcgaaggc
     4621 gcaggaactg ggcattgaag tcatcgacga agcggaaatg ctgcgtttgc tgggtagctg
     4681 agatggaaaa agagcagctg attgaaatag ccaatacgat aatgccgttt ggcaaataca
     4741 aagggcgtcg cttaattgac ctgccggagg agtatctgct gtggtttgcc cgcaaagatg
     4801 agttcccggc agggaagctc ggtgagctaa tgcaaatcac gctgctgatt aaaaccgagg
     4861 ggctgacgca actggtccag cccctgaagc gtccgcttta agctttatcg gcgctgcttt
     4921 cctgctgcgc ctgtaactgt tcggtctggc gtttgtatcg acgcgctagc accgcacaga
     4981 ccatcaattg gatctgatgg aaaatcatca ggggcagcac catcataccg atcaccgatg
     5041 tggggaacag aatgtttgcc atcgggatgc catttgccag actctttttc gaaccacaaa
     5101 agacgatagt aatttcatct gccttattga agcccagtcg gcgtgccata aagacgttaa
     5161 ctacaatcac gatagccaga agaacgcagc tgaccaccac gataaacagc aatgatcccc
     5221 agccaacttt atgccagata ccattaacga cggcttcgct gaacgctgta taaaccacca
     5281 acagaatgga cgtctggtca gttttcgcaa tccatttttt attgcgcgac acccagtcac
     5341 caatccacgg ccgggaaaga tgccccaaca caaacggcag cagcagttgc agcataattt
     5401 taccgacctg ctcaaggctg ccccttgcac cgtgaacgtt catcaccaga ccaaccagca
     5461 atggtgaaag gaaaatcccc agcaggctgg atgctgacgc agaacaaacc gccgccgcga
     5521 cgttaccgcc cgccattgac gtgaaggcga ttgcagactg cacggtagcc gggagaatgc
     5581 acaagtagag aaaaccggag tagagcatcg ggtcgacatt taccggtttc caccaggcaa
     5641 acagtacacc cagaatcgga aacagcacga aggtgctgca cattacccac aaatgcagtc
     5701 gccagtgacc accgccagca ataatcgcct cacgcgacaa cttcgcgccg tgcataaaga
     5761 acagcagggc aatagctgcg gtggtcagat tttcaaagaa ggggacgaaa tcgcctctgg
     5821 ccggaaagaa agaggccagc aacaccaccg tgatcagggt taaggtgaaa ggatcgagga
     5881 tacgaaaaag tttcataaag actccagatg atcgatggtt ctattgtgct tcttccagtt
     5941 tgagaaataa aattgattta ttgcatccat tcatgaagaa aacagatgaa ttattcttta
     6001 aaacaattaa aggttttcgt cacagtagcg caggagaaaa gttttagtcg tgcaggagag
     6061 cgtatcggct tgagccagtc ggcagtaagt cacagtgtga aggagctgga aaatcatact
     6121 ggtgttcgcc tgctggacag aaccacgcgt gaagtggtgc ttacagatgc agggcagcag
     6181 ttggctttgc gtcttgagcg actgctggat gaacttaaca gcacgttgcg cgataccggg
     6241 cgtatggggc aacaactgag cggaaaagtt cgggtcgctg ccagccagac catttccgcg
     6301 catcttattc cgcaatgcat tgctgaaagc catcgccgct atccagatat tcagtttgtc
     6361 ctgcatgatc gtccgcagca gtgggtgatg gaaagtattc gccagggaga tgtcgacttt
     6421 ggcatcgtca tcgatccagg cccagtgggc gatctgcaat gtgaagcgat tctttccgag
     6481 cctttctttc tgctttgcca tcgcgatagc gctttggccg tggaagatta tgtaccctgg
     6541 caggcattac agggagcgaa attggtgttg caggattacg cgtcaggcag ccgaccgctg
     6601 attgacgcag cgctggcgcg caatggtatt caggcgaata ttgtgcagga gattggacat
     6661 ccggcgacgc tgttcccgat ggtagccgca ggcatcggca tcagtattct tcctgcactg
     6721 gcgctaccat tgcctgaagg tagcccactg gtggttaagc gcattacgcc ggtggttgaa
     6781 cgccagttga tgctggtgcg caggaagaac cgttcacttt ccaccgccgc tgaagcgtta
     6841 tgggacgtgg tacgagacca gggcaatgcg ctaatggctg gtcgggaagg ggatccgttg
     6901 tatcagatat agatatcgat atgagtcgtt ttttcaacag gatttagaag ggatacattc
     6961 agagacaaac gctgctcttt ctcctgcgcc ttttctgcct gctgtttctg caactgcgcc
     7021 agttgtgttt ccagtagggt gatttgctgt tgaatcaatt cggcctgttt ttgcttgtct
     7081 tcagcactgc cgctagtgtc tacaatctct ttaatttgtt gagtgagttt gatgatttgc
     7141 tgacttatcc gcgcgatttg tgaagcaaca tcgctacctt gtgatgccct gttactactc
     7201 tgtgtttgta tggaaggaat tgaagttgaa gagatagtgg ccattgttca gtcctcgtca
     7261 ctgtacgagg taatatcggt agaagaacaa ggaacttgag aagaaaaatc aatcttacaa
     7321 atcgcagata gcaaaaaagc gcctttaggg cgcttttttg cattggtggg tcgtgcagga
     7381 tgactcgctt cgctcgccct tcgggccgtc gccgcgagcg gctccgttgt ttcgctgcgc
     7441 tcaactcaaa cctgctgcag gttcgaatct ttgagtatcg cagatagtaa aaaa
//
LOCUS       DADLTI010000175         7474 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLTI010000175 DADLTI010000000
VERSION     DADLTI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.177"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            18..1076
                     /locus_tag="JN755_003562"
     CDS             18..1076
                     /locus_tag="JN755_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7382219.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="JN755_003563"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="JN755_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7382220.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="JN755_003564"
     CDS             complement(1623..1727)
                     /locus_tag="JN755_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382221.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="JN755_003565"
     CDS             1897..2343
                     /locus_tag="JN755_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7382222.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3121)
                     /locus_tag="JN755_003566"
     CDS             complement(2300..3121)
                     /locus_tag="JN755_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7382223.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3218..4399
                     /gene="nimT"
                     /locus_tag="JN755_003567"
                     /pseudo
     CDS             3218..4399
                     /gene="nimT"
                     /locus_tag="JN755_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4454..4801
                     /locus_tag="JN755_003568"
     CDS             4454..4801
                     /locus_tag="JN755_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7382224.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4823..5077)
                     /gene="yoaF"
                     /locus_tag="JN755_003569"
     CDS             complement(4823..5077)
                     /gene="yoaF"
                     /locus_tag="JN755_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7382225.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5260..6285
                     /gene="dgcP"
                     /locus_tag="JN755_003570"
     CDS             5260..6285
                     /gene="dgcP"
                     /locus_tag="JN755_003570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY7382226.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6318..6416
                     /gene="yoaK"
                     /locus_tag="JN755_003571"
     CDS             6318..6416
                     /gene="yoaK"
                     /locus_tag="JN755_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY7382227.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6419..6493
                     /gene="yoaJ"
                     /locus_tag="JN755_003572"
     CDS             6419..6493
                     /gene="yoaJ"
                     /locus_tag="JN755_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY7382228.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6545..6976)
                     /locus_tag="JN755_003573"
     CDS             complement(6545..6976)
                     /locus_tag="JN755_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382229.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6970..7197)
                     /locus_tag="JN755_003574"
     CDS             complement(6970..7197)
                     /locus_tag="JN755_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7382230.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1834 a         1795 c         1806 g         2039 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tagccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacaa cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttatccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccctag gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc agtcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatt aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
     2881 tcacttgatt gctatgcttc accccgcccg gtatccaaac cgcatattgt ggcggcacca
     2941 tccacaaagc attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc
     3001 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
     3061 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
     3121 ttttgtccga acttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
     3181 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
     3241 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacaa cattacgcgt
     3301 cacctttacc ggcgcagcgc cgttactgga tacgattcgt tccgcttact cgctgacgac
     3361 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcacctct
     3421 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
     3481 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
     3541 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
     3601 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
     3661 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
     3721 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
     3781 gcgaagtcag caacatgcaa atttgagtac ctcacgcgcc ttacatactc ggggtatctg
     3841 gcgttcgcca cttgcctagc aggtaacatt gtttcttggg atcaactcac tggtctatta
     3901 cgtgattatt ggctggcttc cggcgatcct catcagtcac ggctatagcg aagcacaggc
     3961 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatcccact
     4021 tttcttacat catgtgaaag accagcgtgg tattgcagcg ttcgttgctt tgatgtgcgc
     4081 agtgggcgcg gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
     4141 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
     4201 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
     4261 agcctgtggg ccgccgctaa tgggtaaaat acacgatgct aacggtaact ggtccgtacc
     4321 actgatgggt gttgcaatac tttcactact gatggcgatt ttcggacttt gcgccgggag
     4381 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatctt gcgtgaaagg
     4441 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
     4501 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
     4561 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
     4621 cgaagtcgtc gattttgcaa cctttcgcga gcaatatcgc gcagaactgg cgcaacacga
     4681 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctacgc
     4741 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
     4801 attttagtac ggcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
     4861 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
     4921 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
     4981 taccaatctg cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga
     5041 ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc
     5101 aagcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
     5161 aaacatatta cgtcttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
     5221 aataatcagc acttacccgc cgtatcctgg agttgttccg tgtcagatca gattatcgcc
     5281 cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gtctggtccg acagcttctg
     5341 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
     5401 gcgcgcctgc aacatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
     5461 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ctgctttttc
     5521 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc
     5581 acatttctga gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
     5641 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc
     5701 ggactgattg cacaatatat tcaaaaagag gcactggttg aacagctgca cgaagccaat
     5761 gctgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
     5821 atttttgaaa atctgacgac gctgttttcc ctcgcccggc atcttaacca taagataatg
     5881 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg atcgttttgg tcataatagt
     5941 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
     6001 gttattggtc gtctcggtgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg
     6061 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt
     6121 gatgttgatt tgtattatcc cggtgccagt cttggcatag tagaagtcga tcctgaaaca
     6181 accgatgcag acagtgccct gcatgctgcc gatattgcga tgtatcagga gaaaaaacac
     6241 aaacagaaaa caccttttgt cgcgcatcca gcgctacatt cctgaggcgt attcacatcc
     6301 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc ttcattacag
     6361 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
     6421 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct
     6481 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
     6541 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
     6601 acggctgaag catttcgtct gcagtctgaa ccgcaggcaa ccatttttca gcaagtctgc
     6661 gctgggaatc gctggcttta tcccatttgc tccacagtgc ctcacgatcg taatcgtgcg
     6721 cttccagcgt ggggcgggcg atttcaaaat acccctggct tgtttctatt tgctcagatt
     6781 ttaataacaa ctcaacacgt gctgccgctg gtagaactga tatatgaaat tccaaagcct
     6841 ttgaacccgt acgcttgcga cgtagttctg gatgattggc tgcaagcttt tcaagattgc
     6901 ggcgaatact cacctcatgt ttaggaaagc atgacattcc tacgcattct tgcgctgtta
     6961 cccaatgggt taacatgatg ttttctccat ataccgagaa ggccaaatct gggctggttc
     7021 catatccaaa tatctagcaa tgattgcttc gtatcgtggt atatggcagc gaaatacgtt
     7081 acgcatagtg tcaggcttta agcctgcctc tctggataat tgagatagtg tcttcccctt
     7141 catacgaaca gcagccttta tctgctctgc gtgccagtct tggttttttg ctgacattct
     7201 gcgatcctta ctctcttaac gttttcggta actgataccg gctaccgcta tcgggaaaaa
     7261 cgatatcgca gaaaaatgcg catttcaaga gctatgcaca tttttatttt aaccaatgca
     7321 aatcaaagaa ttctgtaaaa ttctttatat aacagagaga ttgagcatga caggtgaaaa
     7381 agaaagcaaa aatgcagagc gcatttttat gccccggaaa taatgcgttt caaggaacgc
     7441 cttgaagagg caatgaatgg agaatcaagc cggg
//
LOCUS       DADLTI010000176         7459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLTI010000176 DADLTI010000000
VERSION     DADLTI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.178"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..233)
                     /locus_tag="JN755_003575"
     CDS             complement(<1..233)
                     /locus_tag="JN755_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382231.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JN755_003576"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JN755_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7382232.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JN755_003577"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JN755_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7382233.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JN755_003578"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JN755_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7382234.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JN755_003579"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JN755_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JN755_003580"
     CDS             complement(3305..3424)
                     /locus_tag="JN755_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7382235.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JN755_003581"
     CDS             complement(3385..3570)
                     /locus_tag="JN755_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382236.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JN755_003582"
     CDS             3671..3844
                     /locus_tag="JN755_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382237.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JN755_003583"
     CDS             3906..4190
                     /locus_tag="JN755_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7382238.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JN755_003584"
     CDS             4194..4529
                     /locus_tag="JN755_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7382239.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JN755_003585"
     CDS             complement(4586..6907)
                     /locus_tag="JN755_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7382240.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JN755_003586"
     CDS             complement(7024..7233)
                     /locus_tag="JN755_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382241.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2047 a         1752 c         1654 g         2006 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt ttgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaacaaa taactcttc
//
LOCUS       DADLTI010000177         7458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLTI010000177 DADLTI010000000
VERSION     DADLTI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.179"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            214..290
                     /locus_tag="JN755_003587"
     tRNA            214..290
                     /locus_tag="JN755_003587"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Arg,seq:tct)
     gene            complement(336..1097)
                     /locus_tag="JN755_003588"
     CDS             complement(336..1097)
                     /locus_tag="JN755_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7382242.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1280..2170)
                     /locus_tag="JN755_003589"
     CDS             complement(1280..2170)
                     /locus_tag="JN755_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7382243.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JN755_003590"
     CDS             complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JN755_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY7382244.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JN755_003591"
     CDS             complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JN755_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY7382245.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1737 a         1938 c         1951 g         1832 t
ORIGIN      
        1 ttccgttagg gcggtaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga
       61 cgcgcagcgg tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg
      121 tcgttcactt gttcagcaac cagaccaaaa gccattgact cagcaagggt tgaccgtata
      181 attcacgcga ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca
      241 acgaccttct aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc
      301 atttatgccc gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt
      361 ttggcgagat aattcaacgg tgtcaggctg taaaacgcct taaaaacaga gataaagtac
      421 gacgtgctgc tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat
      481 aacatctgta cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt
      541 tcgtttttta atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt
      601 cgcagattcc agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg
      661 ctgctgcgta agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac
      721 aatacggtaa aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct gggttgctgc
      781 ttgctgtggt tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc
      841 ggggttgcca gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga
      901 taatagttga tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt
      961 tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta
     1021 gcatggttat tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca
     1081 ctgctgctca attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat
     1141 gcttcctgca tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt
     1201 tttagcaatt aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc
     1261 cgacttacct ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca
     1321 gagagaaata gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt
     1381 ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat
     1441 aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt
     1501 cacgctgttc agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc
     1561 cggttgcttt aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga
     1621 aaaaactact gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt
     1681 ttaaccatgt tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt
     1741 ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt
     1801 gctggagatc ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt
     1861 tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct
     1921 gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat
     1981 cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca
     2041 tcagcccctg ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa
     2101 tacctttcat cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga
     2161 acttacgcat ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac
     2221 gttgattaat cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc
     2281 aggcgtcgta gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct
     2341 gcgcgccctg agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa
     2401 tagtctgtcc gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct
     2461 gaatatcctg gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg
     2521 aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc
     2581 gcagcatggt atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga
     2641 ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt
     2701 tcaccggcat catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga
     2761 gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt
     2821 agctacgata gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg
     2881 cgccggaacg caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct
     2941 catgcaaacg gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatca
     3001 gcgcctgatt atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt
     3061 catccagacg ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc
     3121 ctttatgcgc ctgttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg
     3181 cgtaaccaag cgctgcctgg gtgttgctat tattcggttc cagttccagt tccagcgcgg
     3241 cgcgcaaatc actcaccgcg gccggaacat tatgacgttg gcgataaatt gtcgcccgcg
     3301 caacgtaagc gttggcagaa ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga
     3361 gttctggctg agctgggata taacgttgcg catgcagcca ccagtattga gcattgtttc
     3421 ccagctcacg ttgttctgcc tgttgaagcc agcgatcgcg agccgcgcca tttcccgctg
     3481 cctgagcggt gttggcggcg gcgagcagat cctcattgct catgtcgtga agactgattt
     3541 tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg atacgcctga taggctaccg
     3601 cacgatgttg ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca tacaacgcca
     3661 caccgggtag cgtgtcccga taacactttg ccagacggtt ccaggcggca gcatcgtagg
     3721 aaggcgacat atcgcccagc aagcgaacta ttgccgggca attatctgca atacccggca
     3781 actgactttg ccactgacgt tgctccgcca gcggtaaggg tttcgataaa atcgccacct
     3841 tcgccggcgt tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc gccattaaag
     3901 tctggctgac acgcgcatcg ccctggaaag gatagcgttg cagcaataaa tcggcagctt
     3961 cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg atccaggcgg ggaagatttg
     4021 tcggttcttg ctgatacagc aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg
     4081 ccacgctgac agcataacgc tcctcaagca tttcattggc ggggagggtg gcgagcagtt
     4141 tttgcgctgc gtcgtactga ccctctttta acagcaccgg tagcgtcgcg ccaacaacat
     4201 actggcggtt atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc
     4261 tgtatttaga taacagatag agccaacttt tctcttgtgc gcccgtggta aacagcggct
     4321 tattttcaat gagataatgc tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg
     4381 cgtaagtaat atatgactga ggatcggtga agatcccctg tgattgcagt gccaggatcc
     4441 gatcgtccag ctgcccggca agaagcacgt caaaccactg acggcgttct gccgcgctta
     4501 atgtgttctg ctggcgtgct tcattgtata gcgtatctgc ctgggaccat tgtttcaggt
     4561 agattgcccg ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg
     4621 tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc cagccgcagg gcattctgcc
     4681 cgacttcact gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca
     4741 gttcttcaac agtcgtaacg cttttcactt caactggaat agccgccaga ctgcgctcaa
     4801 gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag caacagccgc gcccggtcat
     4861 catgaccaaa atgacgatag gcttccgcaa ggtataaagt cagcggaata ttatccggca
     4921 cctgttggtg tatatattca aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg
     4981 ccttcagcgc cttatcgaga cggggataaa taacaaaatg gcgataatcg ctcagcccca
     5041 gctcttctgc gctggtgccg atattgtctg cgagtgcgct ggtactcaat aaagacgtca
     5101 gcagtaaacc agaccatccg atgacgcgat taaggttatt ctccttcatt ttcggactcc
     5161 agttgcgcaa cctgttctgt gtttaaacct gctttgagta atagtgattg catcgaaact
     5221 tgtaattcgc gttgaattgt caggacgcga tccaacgttt cctggctgat aacgccttcg
     5281 gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat ggcgcaataa caacacgtta
     5341 attgctgaac gattaatatg accgagcgtg gtcagtattt cggcgaacag gaactgatgc
     5401 ggcacatatt gccgccagat ttcaccggtc tgctgttccg tgagccactg atgctgaacc
     5461 gcattgtaca acattgcccg cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac
     5521 cctgtgacaa tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc gactttacgc
     5581 gtcagggccg ccagcgaaac cgggtcaata ccatcttcac tgccgacaat taactcatca
     5641 ttttccagac gcagcggcag tactgcataa tgcagcgcta cggaggctgg catttcggca
     5701 atcagcgagg acgggatctg ccaggcatcg atggattccc acgccacgcc gttttgctct
     5761 gcctgcgcct gtgccagctg ctcggcgcta atcagcccct gcatcagcat tgaaccgccc
     5821 aggcgtagac cttcgacgcg attacgcagt gctgtatcga gttgttcttc agtgatgacc
     5881 tgattttcca gcagaatttg acctaacggg cgcaacgagc gggtatcgcc agtgacgctg
     5941 gggaagtcat gcgttgtttt atcccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgcca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc tgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agccgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaa
//
LOCUS       DADLTI010000178         7359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLTI010000178 DADLTI010000000
VERSION     DADLTI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.180"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            59..460
                     /locus_tag="JN755_003592"
     CDS             59..460
                     /locus_tag="JN755_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7382246.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JN755_003593"
     CDS             570..1901
                     /locus_tag="JN755_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7382247.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIYLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /locus_tag="JN755_003594"
                     /pseudo
     CDS             2225..3613
                     /locus_tag="JN755_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            3695..7328
                     /locus_tag="JN755_003595"
                     /pseudo
     CDS             3695..7328
                     /locus_tag="JN755_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
BASE COUNT         1971 a         1810 c         1675 g         1903 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattta tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaa
//
LOCUS       DADLTI010000179         7356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLTI010000179 DADLTI010000000
VERSION     DADLTI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.181"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            26..877
                     /gene="hchA"
                     /locus_tag="JN755_003596"
     CDS             26..877
                     /gene="hchA"
                     /locus_tag="JN755_003596"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7382248.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(985..2343)
                     /locus_tag="JN755_003597"
     CDS             complement(985..2343)
                     /locus_tag="JN755_003597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7382249.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNIIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2343..3014)
                     /gene="hprR"
                     /locus_tag="JN755_003598"
     CDS             complement(2343..3014)
                     /gene="hprR"
                     /locus_tag="JN755_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7382250.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3147..3560
                     /gene="hiuH"
                     /locus_tag="JN755_003599"
     CDS             3147..3560
                     /gene="hiuH"
                     /locus_tag="JN755_003599"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7382251.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3669..4673
                     /gene="msrP"
                     /locus_tag="JN755_003600"
                     /pseudo
     CDS             3669..4673
                     /gene="msrP"
                     /locus_tag="JN755_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4674..5308
                     /gene="msrQ"
                     /locus_tag="JN755_003601"
                     /pseudo
     CDS             4674..5308
                     /gene="msrQ"
                     /locus_tag="JN755_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5566..6216
                     /gene="zinT"
                     /locus_tag="JN755_003602"
     CDS             5566..6216
                     /gene="zinT"
                     /locus_tag="JN755_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7382252.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7294..>7356)
                     /locus_tag="JN755_003603"
     CDS             complement(7294..>7356)
                     /locus_tag="JN755_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7382253.1"
                     /translation="NRVDTSTAPDIEWPEEPDTM"
BASE COUNT         2071 a         1671 c         1541 g         2073 t
ORIGIN      
        1 actaagcaac aataaggaat acactatgac tgttcaaaca agtaaaaacc cgcaggtcga
       61 tattgctgaa gataatgcgt tcttcccttc agaatattcg cttagccaat atacgagtcc
      121 cgtctctgat cttgatggcg tggactatcc aaaaccgtat cgcggtaaac ataaaattct
      181 ggtgatcgcc gcggacgaac gttatttgcc gaccgataac ggaaaactgt tctcgaccgg
      241 taaccatccg attgaaacgt tgctgccgtt gtatcatctc catgctgcag gtttcgaatt
      301 cgaagtggcg accatttccg gtctgatgac caagtttgaa tactgggcta tgccgcacaa
      361 agatgaaaaa gtaatgccat tctttgaaca gcataaatcg ttattccgta atccgaagaa
      421 actcgcggat gttgtcgcca gcctcaacgc tgatagcgaa tatgcagcaa tctttgttcc
      481 tggtgggcat ggcgcactta ttggtttacc agaaagccag gacgtggctg ccgctttaca
      541 gtgggcaatc aaaaatgacc gttttgttat ctccctttgc cacggtccgg cggcttttct
      601 ggcacttcgc cacagcgata acccactgaa tggttattct atttgcgcat tcccagacgc
      661 cgcagacaaa caaacgccag atattggcta tatgccgggt catctcacct ggtacttcgg
      721 cgaagagttg aagaaaatgg gcatgaacat cattaatgac gacatcaccg ggcgagtaca
      781 taaggaccgt aaacgtctca ccggcgacag tccttttgca gcgaatgcgt tgggtaaact
      841 ggcggcgcag gaaatgctgg cagcttacgc gagttaatcc ctgttacatt gtgatgtaag
      901 cgcataatca atcgcagcca cgttaatact cacgacgtgg ctgcgttttt tcacggttaa
      961 cttatggcgt actgaagccc gatgttaatt tctttgcggt aacgtaattc ggaacacgtt
     1021 atgcttactg agatagtgat acgtagcact tcctccatgt aattcggcaa tcgctttgac
     1081 taaagaaagg ccaagtccct gacctacgga atgacgcgaa ttatctcccc gccaaaatct
     1141 acggaagagt ttttcaggct cattaatttt cgttccagga ctggcgatat caatattaag
     1201 agagccgttg gcatcaagaa aactggttat atgaatacgc gatttttctg gtgaatatcg
     1261 aatggcgtta acaataatat tcgataacat tcgttgcagt aaaattttat ccgcaaagat
     1321 ttgttgattg cacttgacct taaagcaaat ctctttctcg tctgaaagag attcaagata
     1381 gtccaaaaga ttttcaactt ccttattgag agaaagcgaa tccagtttca ccaaaacatt
     1441 gtttttgtct gccctggcaa gaaacagtat attctctgtt aaccgcgaaa tattttccag
     1501 ctcttcaatg tttccggcaa ttgttttttg atattcagcg atacttctga tttgactgag
     1561 cgtaacctga ttctgaccca gtaatgcatt aattggcgtt ctaagttcat gagcgagatc
     1621 gtcagcaaat tgactaaggc gctcaaaatc tttgactaaa gcatgatgca ttttattcaa
     1681 cgcctgccca agaggtttta gttctctcgg taatgcgtta acctcaaccg gcactcggct
     1741 atcgttataa ttcagcgctt ccgttacgcc actcaacttt ttgatctctc gcaaccccgt
     1801 cctgattaac aacggactta atactgagca cagtataatg gcgacaatgc aaattataat
     1861 gctattaatt ttatactgtt caagcatatt atgtctggct gaagccaatt tagccacagt
     1921 aaccgttaat gatggcgaaa cttcatcaat gttaattcgt aaagcatcta tctctgtatc
     1981 attaataatg cttctgtaaa tgcctgctgc gctgattgtc tcactagcag gtatgttatt
     2041 taacatgtca tcactgacat ttgtccggtt aacaatttta ttgatgccat cactatgaat
     2101 gatcaaaata tcctgactaa catccatcat ccggttaaag tacacaggta acgtatctgg
     2161 atttacccca tcaattaaca actgcttgat ctgcgctgtc cggttaatga gtgttgtatc
     2221 atcgcgccat ttcaactcac ttgccaggcc attatagaga gtccatacaa ttccagcgcc
     2281 agcaacagac tgcagaaata taaaaagcaa ggttaaacgg acggttatag ataatctttt
     2341 cattattttt ttaccgctac gaatgaatag cccatccccc ggattgtggc gattagcttt
     2401 tcaggaaaag gatcatcaac ttttgcgcgg agcctgcgaa tggcgacatc caccgtattg
     2461 gtatcactat caaagttgat tccccaaatt tcactcgcaa taaccgttct gggtataatc
     2521 tcgccagctc tggaggccag tagccaaagt aactgaaact ccttgcgcgt cagtgtaata
     2581 ctgatattgt ccctgctgac actttgactg acagagtcca tttttaagcc actgatttct
     2641 aatgttgaat tcagagcgtg atgttgcctt aattgtgccc gaacccttgc taacaactca
     2701 gaaaatgaaa aaggttttac cagataatca tttgccccac tgtccaaccc tctgactctg
     2761 tcatcgacag aatcccttgc agtaaggcaa ataacagggg tttgctttgc cgttcttaac
     2821 gtttgtaaga tctgccagcc atccataccc ggaagcataa tatccagaat gatcaatgca
     2881 taatcatcct tcagcgcaag ataaagccca tctctgccat cagaaacggc atcaatgaca
     2941 taacccgctt cggaaagccc ctgcgttacc cattcctggg tcctttgatt atcttcaata
     3001 agtagaatct tcatgaaaat aatatgccat aaatcatatg ttatagaaac aacctggttc
     3061 attacaaaat tgtaatgctg ctgtaaggtt accctggacg ttttttcgct atcctcaaaa
     3121 ctcattcaca tgacaaggat ataaacatgt taaagcgtta tttagtactc tcggtagcaa
     3181 cggcagcatt ttcattacct tctttggttt atgccgcaca acaaaacatt cttagcgtgc
     3241 acattttgaa ccagcaaaca ggaaaacccg ctgccgacgt gacagtcact cttgaaaaga
     3301 aagcggataa cggctggtta caacttaata ccgccaaaac agataaggat ggacgaatta
     3361 aggcactgtg gcccgagcaa actgcgacta ctggcgatta ccgtgtcgta tttaaaaccg
     3421 gggactattt caagaaacaa aatcttgaaa gtttcttccc tgagattccc gttgaatttc
     3481 atattaataa agtgaacgag cattatcatg tgcctttgtt actcagccaa tatgggtatt
     3541 caacctatcg tggcagttaa tgcgtaaata tgtaaaagct gatctctgct actgttaggg
     3601 aggtgactcc ctgacagatt aacagtaaac agctcttgtt ggttaacgac aaaaaagtgt
     3661 gatggcttat gaaaaagaat caatttttaa aagaatcaga tgttacggcc gagtcggtat
     3721 tctttatgaa gcgtcggcag gtgttaaaag cactgggcat cagcgcagct gcattttctt
     3781 tgcctcacgc tgcgcatgcc gatctgctta gctggtttaa agggaacgat cgtccgcccg
     3841 cccccgccgg aaaaccgctg gagttcagca agcctgccgc ctagcaaaat aacctgccac
     3901 tgacgccagc agataaagtc tccggttata acaacttcta tgaattcggg ctggataaag
     3961 acgatcccgc ggctaatgct ggtagcctga aaaccgatcc atggacactg aaaatcagcg
     4021 gcgaagtggc aaaaccattg accctcgatc atgatgattt aacccgtcgc ttcccgctgg
     4081 aagagcgtat ttatcgtatg cgctgcgtgg aagcgtggtc gatggtggtg ccgtggattg
     4141 gttttccgct acacaaattg ctggcgcttg ccgaacccac cagcaatgcg aagtatgtcg
     4201 ctttcgaaac gatttatgcg ccggaacaga tgcctggcca gcaggaccgc tttatcggcg
     4261 gcgggctgaa atatccttat gtcgaaggat tgcgtctcga cgaagcaatg catccgctca
     4321 cactgatgac cgtaggtgtt tatggcaagg cgttaccacc gcaaaatggc gcgccggtgc
     4381 gactgattgt gccgtggaaa tatggcttta aagggattaa atcgatcgtc agtattaagc
     4441 tgacccgcga gcgtccgcca accacctgga atctggcagc gcctgacgaa tacggttttt
     4501 acgccaacgt taatccgcat gttgatcacc cgcgctggtc acaggctacc gaacgattta
     4561 ttggttcagg cggcattctc gatgttcagc gccagccaac actactgttt aatggttacg
     4621 ccgaccaggt ggcatcgctg tatcgtggcc tggatttgcg ggagaatttc taaatgcgtc
     4681 tgacagcaaa acaggtgata tggctgaaag tttgcctgca tcttgccgga ttgttgccgt
     4741 ttctctggct ggtctgggcg atcaatcacg gtggactggg tgccgatccg gtgaaagata
     4801 ttcagcattt taccggtcgc actgcactga aatttttgct tgcggccttg ttaatcacac
     4861 ctctggcacg ctacgcaaaa cagccgttat tgatacgcac tcgccgcctg ttaggcttat
     4921 ggtgctttgc ctgggcgaca ctgcatttaa ccagttacgc attgctggag ttgggcgtga
     4981 ataatctggc gttattaggt aaggagttaa ttacccggcc ttatttaacg ttaggaatta
     5041 tcagctgggt tattttgctt gctttagcgt tcacttcaac ccagtcgatg cagcgaaaac
     5101 tcggcaaaca ctggcaacag ttgcataact tcgtctatct tgtcgcgatc ctggccccaa
     5161 tacattatct gtggtcagtg aagattattt caccgcagcc actcatctac gctgggctgg
     5221 ctgtactgct ttagccttac ggtataagaa gttgctttcc cttttcaacc ggttacgcaa
     5281 gcaggcccac aacaaattat ctctataact tcatattgcc gacaaacttc gcgttatccc
     5341 ggccggatgc ggcgcgagcg ccctatccgg cccacacaga tcgcatcaag ttatgtttcg
     5401 tttgtcatca acctttcatc accagaataa tttattttat ttcaataaga tagttcaata
     5461 cacctgagaa taccatgctc tcatttccta atgagttgtt gcattttttc atatgttaca
     5521 atataacatt acacataatg tacattaact ctggaggaaa ttgttttggc gattcgtctt
     5581 cataaactgg ctgttgcttt aggtgtcttt attgttagcg ctcctgcctt ttcgcatggt
     5641 catcattctc atggcaaacc cttaacagag gtcgaacaaa aagctgctaa tggtgttttt
     5701 gatgatacca atgtacaaaa ccgaacgctc agtgactggg atggagtctg gcaatccgtt
     5761 taccctttac tgcaaagtgg caaacttgat cccgtctttc agaagaaagc ggatgcagat
     5821 aaaaccaaaa catttgctga aattaaagat tattatcgca aaggttatcc gacagatatc
     5881 gaaatgatta gcattgagga cggcattgtc gaattccata gaaataatga aacaacatcc
     5941 tgtaaatatg attacgatgg atacaaaata ctcacctata aatcaggcaa gaaaggcgtt
     6001 cgttacttat tcgaatgtaa agatcctgaa agcaaagccc ctaaatatat acaatttagc
     6061 gatcatataa ttgcaccacg aaaatcatct cattttcaca tttttatggg taatgattcc
     6121 cagcaatctt tattgaatga aatggaaaac tggccaacgt attatcccta tcagttgagt
     6181 agcgaagaag tggtcgagga gatgatgtct cattgatttt tttgcgctga cttcagttat
     6241 ttaacagaac agtgcctggt agtaacaaca ataatttacc aagacaaata agtaagacaa
     6301 actcagacac gctcatactt gagtcgtgtc ttttttatta tgtctgtaga aaaaaactga
     6361 gaaaaaggcg aacaataaat acacaactca gccatacaga ctggcagcgc aaatgtttcc
     6421 aggagcaagt catctgtcat ggtaaagcta accagaaata taggttaatt ttgttgttta
     6481 aacctttttt acttgttagt ataatacaaa ggctttcaaa aaagctggga aattcaacaa
     6541 ctcttggaag ttatggactt tgtcccgcgg ctttaatctg acggccgcgt tccttttttg
     6601 gctcaattat tgaccccttc tctacgcaac ttcagttccc gccagcaact ttgcggcagt
     6661 ttcataggat caatgtctaa aagaataatg gtgaccgata agaaaacgac tgaataactg
     6721 tagattttcg ctcgaaacct tcctgtcagc tccatagcga atcaagcgct gaatgtcaca
     6781 gtatcgaaca gaaaacagtg acgatctaac ccttcaagaa tattctacga ttgttctgtt
     6841 taggaaaagc aaggcgggaa gtcgggagat aagtcattga taaagtggcg gagagagggg
     6901 gatttgaccc cccggtagag ttgcccctac tccggttttc gagaccgatg atacagagtc
     6961 gaaaaatcaa agagttaaaa atttttcttg gaaaaaatgt cttaaaatcg tacacgaaaa
     7021 atcaatgaat tacataacat taaagaagaa tattccaagc cttcttgagt ccaccacctg
     7081 ttttcaggtg tcattcagac gtgatttgcc ccagattggg aaagcacgct tttttctcag
     7141 cttattccgt tttgcctgtg tgttgaataa ttccactgca gttatataaa atcggtaacg
     7201 gctggaaatc attcaatact cgcactatcg aaagtttacc agccaaccgc agcacgtcct
     7261 gcatacgtcg tgtctgcggt ttttcttttt cgcttacatt gtgtctggtt cttccggcca
     7321 ctcaatatca ggtgcagttg atgtatcaac acggtt
//
LOCUS       DADLTI010000180         7313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLTI010000180 DADLTI010000000
VERSION     DADLTI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.182"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JN755_003604"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JN755_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7382254.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="JN755_003605"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="JN755_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7382255.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="JN755_003606"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="JN755_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="JN755_003607"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="JN755_003607"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7382256.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="JN755_003608"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="JN755_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7382257.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /locus_tag="JN755_003609"
     CDS             complement(5036..6463)
                     /locus_tag="JN755_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7382258.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="JN755_003610"
     CDS             complement(6486..7178)
                     /locus_tag="JN755_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7382259.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1850 a         1765 c         1934 g         1764 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaa
//
LOCUS       DADLTI010000181         7288 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLTI010000181 DADLTI010000000
VERSION     DADLTI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.183"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JN755_003611"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JN755_003611"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1202
                     /gene="yneK"
                     /locus_tag="JN755_003612"
     CDS             462..1202
                     /gene="yneK"
                     /locus_tag="JN755_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7382260.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1352..2542
                     /gene="ydeA"
                     /locus_tag="JN755_003613"
     CDS             1352..2542
                     /gene="ydeA"
                     /locus_tag="JN755_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7382261.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JN755_003614"
                     /pseudo
     CDS             complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JN755_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
     gene            3444..3878
                     /gene="marR"
                     /locus_tag="JN755_003615"
     CDS             3444..3878
                     /gene="marR"
                     /locus_tag="JN755_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7382262.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3898..4303
                     /gene="marA"
                     /locus_tag="JN755_003616"
                     /pseudo
     CDS             3898..4303
                     /gene="marA"
                     /locus_tag="JN755_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4335..4553
                     /gene="marB"
                     /locus_tag="JN755_003617"
     CDS             4335..4553
                     /gene="marB"
                     /locus_tag="JN755_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7382263.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JN755_003618"
     CDS             complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JN755_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7382264.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5747..6865
                     /gene="ydeE"
                     /locus_tag="JN755_003619"
     CDS             5747..6865
                     /gene="ydeE"
                     /locus_tag="JN755_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7382265.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6992..7087
                     /gene="mgtS"
                     /locus_tag="JN755_003620"
     CDS             6992..7087
                     /gene="mgtS"
                     /locus_tag="JN755_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY7382266.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1901 a         1775 c         1658 g         1954 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
      961 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     1021 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     1081 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     1141 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     1201 aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag aaacggaaaa
     1261 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     1321 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     1381 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     1441 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1501 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     1561 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1621 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1681 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     1741 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     1801 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     1861 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1921 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1981 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     2041 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     2101 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     2161 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     2221 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     2281 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     2341 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     2401 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     2461 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2521 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2581 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2641 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2701 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2761 ccatcagtac ccaatcagcg aaggttcaac tctgacgcac tgtggacgcc gagctaataa
     2821 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     2881 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     2941 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     3001 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     3061 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     3121 cactgttcat gttgcctgcc aggccaagaa atagcgcaac agttgttaat gggttagcta
     3181 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     3241 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     3301 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     3361 gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc ccctgcaact
     3421 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3481 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     3541 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3601 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3661 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     3721 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     3781 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     3841 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     3901 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3961 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatgagcg
     4021 ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
     4081 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
     4141 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
     4201 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
     4261 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
     4321 tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
     4381 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
     4441 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
     4501 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagtttgttc
     4561 tggccccgac atctcggggc ttattaactt cccaccttta ccgctttacg ccaccgcaag
     4621 ccaaatacat tgatatacag cccggtcata atgagcaccg cacctaaaaa ttgcagaccc
     4681 gttaagcgtt catccaacaa tagtgccgca cttgccagtc cgactacggg caccagtaac
     4741 gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa
     4801 ccaacaattg tcgccacaaa cgccagatac atcagcgaca agatggtggt catatcaata
     4861 gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag
     4921 ggaatgattg ggattaaagc gctccagatt accagcgaca tcaccgccgg acgcgttgag
     4981 tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc tgcagccagg
     5041 gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc
     5101 agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg
     5161 aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc
     5221 agtccagcag gcataccaaa gttaatggcg cagaacagaa aggcaaactg cgcaaaactg
     5281 atggttaatc cataccccaa gagcaaattc agtggtactt tcggtcgtgc aacaaaaaag
     5341 atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta
     5401 tgaagcccca ctttgatgac cacaaaattt agcccccata cgaccactac cagtagcgcc
     5461 aacaccccat cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca
     5521 acttagctga atttactttt ctttaacagt tgattcgtta gtcgccggtt acgacggcat
     5581 taatgcgcaa ataagtcgct atacttcgga tttttgccat gcgtccgatg gttaataaca
     5641 acgacgaggc aagaagggcg ctggtagagc gtcgtaggga taagttcatt tgtctgtccg
     5701 gcagtgcgtt tcgttatgtt ttattttaga gatgtaaaga aatagcatgg ctacgctgcc
     5761 atttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc
     5821 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
     5881 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat
     5941 tgccattcct ttagtgaata acgtgacgct ggttgtgctc ttttttgccc tcattaactg
     6001 cgcctattct gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac
     6061 cagcaaaacg aaaatcttct caatcaacta caccatgcta aacattggct ggaccatcgg
     6121 tccgccgctc ggcacgctgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc
     6181 tatctgttcc gcgtttccca tgcttttcat tcaaatatgg gtaaagcgca gcgagaaaat
     6241 catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact
     6301 gttgtggttt acctgctctg gttttctggc ttcttttgta agcggcgcat ttgcttcatg
     6361 catttcacaa tatgtgatgg tgattgctga tggggatttt gccgaaaagg tggtcgcggt
     6421 tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgact
     6481 taacccagct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcggact
     6541 ggtcggtttt attttttccg gcaacaacct gctattgtgg ggtatgtcag ctgcggtatt
     6601 tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc
     6661 gccaggaatg aaagccagct atttttccgc ccagtcttta ggctggcttg gtgccgcgat
     6721 taacccatta gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat
     6781 cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaagggattc gtgcaagacc
     6841 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt aattcagcct
     6901 tcgtttaggt tatctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
     6961 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
     7021 cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga
     7081 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag
     7141 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
     7201 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
     7261 gttgacacag tagcatcagt tttctcaa
//
LOCUS       DADLTI010000182         7255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLTI010000182 DADLTI010000000
VERSION     DADLTI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.184"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..149
                     /locus_tag="JN755_003621"
     CDS             <1..149
                     /locus_tag="JN755_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7382267.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN755_003622"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN755_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7382268.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN755_003623"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN755_003623"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7382269.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN755_003624"
     CDS             1974..2087
                     /locus_tag="JN755_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7382270.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN755_003625"
     CDS             2348..2914
                     /locus_tag="JN755_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7382271.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN755_003626"
     CDS             2918..3493
                     /locus_tag="JN755_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7382272.1"
                     /translation="MKKSLLGLTFSSLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..4587)
                     /locus_tag="JN755_003627"
     CDS             complement(3535..4587)
                     /locus_tag="JN755_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7382273.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4812..5732
                     /locus_tag="JN755_003628"
     CDS             4812..5732
                     /locus_tag="JN755_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7382274.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5904..6101
                     /locus_tag="JN755_003629"
     CDS             5904..6101
                     /locus_tag="JN755_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7382275.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            6125..>7255
                     /locus_tag="JN755_003630"
     CDS             6125..>7255
                     /locus_tag="JN755_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7382276.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT         1892 a         1708 c         1713 g         1942 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttagtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttctcg tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
     3061 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
     3181 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
     4141 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     4201 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
     4261 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     4321 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
     4381 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     4441 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
     4501 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     4561 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     4621 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     4681 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
     4741 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     4801 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     4861 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
     4921 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     4981 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     5041 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
     5101 ggttctggcc ggaccgccga atcgcccgct ggacggaagt gatcggcatg gaacacattc
     5161 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     5221 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     5281 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     5341 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     5401 acgcaccaga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
     5461 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     5521 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc
     5581 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     5641 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     5701 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
     5761 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
     5821 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
     5881 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     5941 aacgaaacct gatcgtcgcc tggctaggct gttttcttac cggggccgcc ttcagtctgg
     6001 taatgccctt cttacccctc tacgttgagc agcttggtgt taccggccac tccgccctga
     6061 atatgtggtc cggtattgtc ttcagtataa tgggttccta aacacatcat tttagatatt
     6121 gaatatgaga cgggcaatca gctacatcag attttcaagt gaacgacaac tgaaagggga
     6181 ctctgtacgc cgacaatcta agctggttac tgactggctt gataagaacc ctgagtttta
     6241 cttggattca tccttgagct ttaaagattt ggggaaaagt gcttttagtg gtaagcacct
     6301 gaaaggtggt ctaggtgact ttttaaccgc tatcgagaaa gggcttgtta aagctggtga
     6361 tacgctacta atcgagagtt tagaccgtct gtcacgtcag gatattgata ttgcttctga
     6421 gctattacgc cgtatcttaa gggctggtgt agatgtagtc acactgagcg atggagaaca
     6481 ttacacacga gaaagtttaa aagacccgtt agccctgatt aaaagcattc tgataatgca
     6541 gcgggcgcat gaggaaagtt tgaggaagtc tgagcgtgtc caagctgcat ggaaccgtaa
     6601 aaaagaactc atctccgaag gtatcaaggt atctaggcgt tgtcctgcat ggttaaggct
     6661 gaatgatgac cgtcgaacat tcacaatcat tcctgataaa gtggaagttg ttaaacgagc
     6721 atttgacctg agattacagg gtttaagctt ttgggctatc accagaactt taaacgatga
     6781 aggtcacttg tcattgaatc aatacacacc caaacaaaag gggtggtctg atactgctgt
     6841 aaagaaacta ctcagaaatc gtgctgtgat tggatgtttc acacctgctg gacgtgaaga
     6901 agttcagggt tactaccccg caataatcag tgagtcacta ttctatcgag tacagcaact
     6961 gaacacaggg caatatggta gggcttctgt atcaagtaat ccgctatcag tcaacttgtt
     7021 caggggtatc atcaaatgtt ctgagtgtgg ggctactatt gttcttggtg gatatgcctt
     7081 aaaacgtttt ggaatgtaca gatgccctaa tcgttcagct aacaggtgta gtgcaaaggc
     7141 tatcagtcgt aagcaaacgg atgcaactat cctttacatg ctcgcattat gcgaccgttt
     7201 tgaaactgaa aatactgaca ctattgatag ccttaaatta cagcgtgaag atttg
//
LOCUS       DADLTI010000183         7085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLTI010000183 DADLTI010000000
VERSION     DADLTI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.185"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            15..4880
                     /gene="traI"
                     /locus_tag="JN755_003631"
     CDS             15..4880
                     /gene="traI"
                     /locus_tag="JN755_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7382277.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            4900..5646
                     /gene="traX"
                     /locus_tag="JN755_003632"
     CDS             4900..5646
                     /gene="traX"
                     /locus_tag="JN755_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7382278.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5701..6261
                     /locus_tag="JN755_003633"
     CDS             5701..6261
                     /locus_tag="JN755_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7382279.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6392..6604
                     /locus_tag="JN755_003634"
     CDS             6392..6604
                     /locus_tag="JN755_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7382280.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            6788..>7085
                     /locus_tag="JN755_003635"
     CDS             6788..>7085
                     /locus_tag="JN755_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY7382281.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1636 a         1779 c         2319 g         1351 t
ORIGIN      
        1 gacagtcaga aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca
       61 gtcgcgatca ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata
      121 acggcgaatg gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg
      181 tgtacgctaa tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg
      241 aggcgctggg ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg
      301 taccggtgga ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg
      361 cctcgctgaa atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg
      421 atcctgaagt cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc
      481 gggcatatcg tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg
      541 cttcacagga cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg
      601 aacgcaaagt gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc
      661 cggaaaatgg tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agcagtgagc
      721 agcttatccc cctcgatcgt gagaaggggc tgttcacgtc cgggattcat atgctcgatg
      781 agctgtcagt ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc
      841 cggagaaaag tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg
      901 accgtccgtc gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg
      961 tggctgaact ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg
     1021 accgtcgctc gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg
     1081 gacgtcgtca gttgctggaa ggcatggcct tcacgccggg cagtaccgtt atcgtcgacc
     1141 agggcgaaaa actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata
     1201 acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg
     1261 ccatgaagga tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca
     1321 ccatcatcag tgaaccggac cgtaatgtcc gctatgcccg gctggccgga gattttgcgg
     1381 ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggagtacgg gaacaggcca
     1441 tactgacaca ggccattcgc agtgagctga aaacacaggg cgtgctcgga cacccggagg
     1501 taaccatgac cgccctgtca ccggtctggc tggacagccg gagccgttat ctgcgggata
     1561 tgtaccgccc gggaatggtg atggagcagt ggaacccgga gacacgcagt catgaccgct
     1621 atgtgacaga acgtgtgacg gcgcagagtc acagcctgac cctgcggaat gcgcaggtcg
     1681 aaacgcaagt ggtgcgtatt tcttccctgg acagcagctg gtcgctgttc cggccggaaa
     1741 aaatgccggt ggcagacggt gagcgtctga gggtgacagg gaaaattccc ggactccgcg
     1801 tctccggcgg tgaccgcctg caggtggcat ccgtcagtga agatgcgatg acggttgttg
     1861 tgccgggacg ggctgaaccg gccaccctgc ctgtgagcga ttcaccgttc acggcactga
     1921 agctggagaa cggctgggtg gaaacgcccg ggcattccgt cagcgacagt gcgacggttt
     1981 ttgcctccgt cacacagatg gcaatggaca atgccaccct gaatggtctg gcccgcagtg
     2041 gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa aaacttgccc
     2101 gccatccctc ctttacggtg gtttctgagc agataaaggc gcgtgccggt gagacattgc
     2161 tggaaaccgc tatcagtctg cagaaagccg ggcttcacac gccggcacag caggccattc
     2221 atctggccct tcctgtgctg gaaagtaaaa acctggcctt cagcatggtg gacctgctga
     2281 cagaggcgaa gtcgtttgct gcagaaggaa ccggttttgc tgacctggga ggggaaatca
     2341 atgcgcagat aaaacggggt gatttactgt atgtggatgt ggcaaaaggc tatggcacag
     2401 gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc catattctcg
     2461 aaggtaagga ggcggtcacg ccgctgatgg agagagtacc tggcgaactc atggagaaac
     2521 tgacatcagg acagcgcgcc gccacccgta tgatactgga aacgtctgac cgtttcacgg
     2581 tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg
     2641 tgaacatgct gccggagagt gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg
     2701 cggtcgggga gatgcgcagc gccggcgtgg atgcacagac actggcgtcc tttctgcatg
     2761 acacgcagca gcgcagcgga gaaacgccgg atttcagcaa cacgctgttc cttctcgatg
     2821 agagctctat ggtgggcaat accgacatgg cacgggcata cgccctgatt gcggccggtg
     2881 gcggtcgggc tgtggccagc ggtgacacgg accagctgca ggccatcgcg cccggtcagc
     2941 ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt catcatgaag gagattgtgc
     3001 gtcagacgcc ggaactgcgg gaggcggtat acagcctgat taaccgggat gtggaaaggg
     3061 cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc acgtctggag ggcgcatggg
     3121 caccggagca ctccgtgacg gagttcagtc acagccagga agcgaaactg gcagaagcgc
     3181 agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt ccccatgaca ctgtatgaag
     3241 ccattgtccg cgactatacc ggcaggacac cggaagcacg ggagcagacg ctgattgtca
     3301 cgcacctgaa tgaggaccgg cgcgtactga acagcatgat tcatgatgca cgggaaaagg
     3361 ccggagaact ggggaaggtg caggtcatgg tgcctgtcct gaacacagcg aatatacgcg
     3421 acggggagct gcgtcgtctc tccacctggg agaataaccc ggatgccctt gccctggtgg
     3481 ataatgtgta tcaccggatt gccggtatca gcaaggatga cgggctgata accctgcagg
     3541 atgcggaagg taacacgcgg ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacgc
     3601 tgtacacccc ggacaccatc cgggtgggga ccggtgacag gatacgcttc acgaagagtg
     3661 accgggagcg cggttatgtg gccaacagcg tctggacggt gacagcggtt tccggtgaca
     3721 gtgtcacact ttctgacggt cagcagaccc gtgtgattcg ccccggtcag gagcgggcag
     3781 agcaacatat tgacctggcc tatgccatca ccgcccacgg tgcacagggg gcgagtgaaa
     3841 cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa actgatggcc ggctttgagt
     3901 cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggcgtacacc gataaccgtc
     3961 agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac gatgtgtttg
     4021 agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg gcgcgggagc
     4081 tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc gggggagaca
     4141 gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg gcactgccgg
     4201 cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc acggatgacg
     4261 gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg gatgcgcagt
     4321 tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat atgcaggatg
     4381 gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagaatc gccggtgaag
     4441 gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt gtggggggat atcccggaca
     4501 acagcgtcca gccgggagcc ggaaatggcg aaccggtcac ggcagaggta ctggcacagc
     4561 ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa attgtccgta
     4621 aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagcaggct gtcagggaaa
     4681 ttgccggtca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg ctgccggaga
     4741 gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg ggaagttgcc cgggaaaatt
     4801 tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg cagaaagaga
     4861 aaaccccggg cggagactga tacaggaaga caaactctga tgacaacgga taacacgaac
     4921 acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc atttctggtc
     4981 tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat ggtactggac
     5041 cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg gcgaggagcc
     5101 tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca tatccggcaa
     5161 ccggccatta accggctgtg gggatgggga attattgccc agttcgcata ttatctggca
     5221 ggctttcctt ggtatgaggg gaatatcctg tttgcctttg cagtggcggc acaggtgctg
     5281 acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccatactcct gatggccctg
     5341 tgggggcctt tgtccggcac cagttacggt attgccgggt tgctgatgct ggcagtcagc
     5401 aaccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg
     5461 gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc cggtcttgtg
     5521 atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa aatcattacc ccgtttctgg
     5581 cccggagact ttttcccgac gttctatgcc tgtcatctgg ctgtgctggg cgttctggcg
     5641 ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt cgtcagaggc agaacacagc
     5701 atgatagagc agaagcgacc ggtactgaca ctgaagcgga agacagaggg aacagcgcct
     5761 gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaagtggaa ggtgaaaaag
     5821 cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg cggcaaaaaa agcgcaggcc
     5881 agacaggcgc tgtccattta tctgaacctg cccacgctgg atgaggctgt gaataccctg
     5941 aagccctggt ggccgggatt atttgacggt gacacgccct ggcttctggc ctgcggtatc
     6001 cgggacgtgt tactggaaga cgtggcgcag cggaatatcc cgctctcgca taaaaaactg
     6061 cgcagggcgc tgaaggccat cacccgttca gaaagctatc tgtgtgccat gaaagccggt
     6121 gcctgccggt atgacacgga agggtatgtg acggagcata tttctcagga ggaggaagcg
     6181 tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc ggataaaggc agaacttcag
     6241 gccgtgcttg atgagaaata aaaaaagcct cccccagaga gaggaggcag ggaaataagg
     6301 cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt atttatcaca
     6361 ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca gttcacggaa
     6421 cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg aagtcagtgg
     6481 acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc ctaggacaga
     6541 ctggcagcag tttgcccgga aaagggctga acactgccac cggcgctgcc ggggaagggt
     6601 gtaacgaaaa aagcagcctt cattactcta ggagtcagaa ggctgaaagg agtttatgag
     6661 atttgcttct taatatccgg tcgggcagta tgaccggaca gttctgagta gcacagggtc
     6721 ttttccgcat taagtcgatt ttttatattt tgtgattaat ttcacaaaat aaggtgttat
     6781 tcaatgtgtg ctgcactatt caggggggct gaaatacagg tggcatttat ctgatatgga
     6841 gaataacatg aggaaatata ttccactggt attatttatc ttttcatggc cggtattgtc
     6901 tgcagatatt catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga
     6961 ctcactgaag gcggtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga
     7021 ttatggccgg tggtcaacag acatgatgaa atcactggtc gcaggaaaaa cggttaccgt
     7081 cacat
//
LOCUS       DADLTI010000184         6949 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLTI010000184 DADLTI010000000
VERSION     DADLTI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.186"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..731
                     /locus_tag="JN755_003636"
     CDS             <1..731
                     /locus_tag="JN755_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7382282.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPSVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1034..1804)
                     /locus_tag="JN755_003637"
     CDS             complement(1034..1804)
                     /locus_tag="JN755_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382283.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JN755_003638"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JN755_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JN755_003639"
     CDS             complement(2295..2516)
                     /locus_tag="JN755_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382284.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JN755_003640"
     CDS             complement(2517..3200)
                     /locus_tag="JN755_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7382285.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JN755_003641"
     CDS             complement(3264..3575)
                     /locus_tag="JN755_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382286.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JN755_003642"
     CDS             complement(3572..4543)
                     /locus_tag="JN755_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7382287.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JN755_003643"
     CDS             4914..5873
                     /locus_tag="JN755_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7382288.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JN755_003644"
     CDS             5873..6268
                     /locus_tag="JN755_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7382289.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="JN755_003645"
     CDS             6451..6831
                     /locus_tag="JN755_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7382290.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1896 a         1802 c         1684 g         1567 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctagtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgccctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga acacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgctt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac tgttaacgct
     6061 aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacac
//
LOCUS       DADLTI010000185         6864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLTI010000185 DADLTI010000000
VERSION     DADLTI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.187"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..92)
                     /locus_tag="JN755_003646"
     CDS             complement(<1..92)
                     /locus_tag="JN755_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY7382291.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            148..300
                     /locus_tag="JN755_003647"
                     /pseudo
     CDS             148..300
                     /locus_tag="JN755_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1681..2628
                     /gene="icsP"
                     /locus_tag="JN755_003648"
     CDS             1681..2628
                     /gene="icsP"
                     /locus_tag="JN755_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7382292.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3190..3562)
                     /locus_tag="JN755_003649"
                     /pseudo
     CDS             complement(3190..3562)
                     /locus_tag="JN755_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3565..3762
                     /locus_tag="JN755_003650"
                     /pseudo
     CDS             3565..3762
                     /locus_tag="JN755_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3806..3925
                     /locus_tag="JN755_003651"
                     /pseudo
     CDS             3806..3925
                     /locus_tag="JN755_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4175..5041
                     /gene="ospB"
                     /locus_tag="JN755_003652"
     CDS             4175..5041
                     /gene="ospB"
                     /locus_tag="JN755_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7382293.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5370..6110
                     /locus_tag="JN755_003653"
     CDS             5370..6110
                     /locus_tag="JN755_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7382294.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6463..>6864)
                     /locus_tag="JN755_003654"
     CDS             complement(6463..>6864)
                     /locus_tag="JN755_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7382295.1"
                     /translation="IKASNYEEINNKVTDKKMAHQALAYSLGDKKADIALYLLSKFNF
                     TKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGD
                     TMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2156 a         1281 c         1327 g         2100 t
ORIGIN      
        1 tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt tattggaaat
       61 acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa atataaacat
      121 taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca ctttatctat
      181 cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcgattacaa tcgctggcga
      241 cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
      301 gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg cgtccatatt
      361 atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag aaaatttata
      421 ccctgcaggt atcataaaca tgcttgataa cttaattggg gctccctctt ccttttatgg
      481 tgtaattgtt tgattgaata cttccgggga tttcagtatg aaatgaagta tatttaatat
      541 actttacaaa aagtttgtat tagtggaata aggacaatag cttatccaaa ctgccaagcc
      601 tctttattat aagtaagatc tggcactgga atattctcaa aaggactaac atcatcacca
      661 cgaccttcaa ccaaccattt tattgcattt aaatcccaga atagttcagt agtggtcaat
      721 ttgggaaacg cacaatattg tacggttacg ttatcgcaat agagtgaact cccgccctat
      781 acggcgtttt caaggattag tccttaatcg gacaaccaca ttaaagcata ataaataatt
      841 aagctttaat aatatactat agatacagtt ctattataaa aaatactatt ttaggtgatg
      901 tattaaatta gtagaatatt tattctttta taaaagcacc aatgaatttt ccaagtaaac
      961 atgaggtaaa atgtgtagag gataactgag tttggtcact ttaactgtat tagtcgcagt
     1021 ctgactctcg actttaaaag gatgggaatt acctgtactg atagtgagaa agctttccat
     1081 tgaaataagt caaataaaag ttgatggctt cagtgctaaa gtgaggtgcc cgctagccgt
     1141 attcactcga atcggaattc aatgtattgc ttctgccatt tccataatat ttcacgggaa
     1201 aaacaaaatc tgaaaaacta tacttaagtg ttgtattaag tcagttgaga tacaaataca
     1261 tatttatcag cacacgttac gtcaaataca ggaggttgtc atgttggtac tgaaaggcac
     1321 gttggctatt gcagccgtga tgacaattta ttgtcttatc gttgttctta tggaacgctt
     1381 ttctgattga tttcatattg gcgagagaac aagaaggtga gtagaattgt cgcggaatcc
     1441 tgaaactatc agcctcaggt atgaacacca aaaggtaggt agagaaatct atggcccccg
     1501 ttactttatt cgcacaatga ggtatttttt tatttttaca aaagttcaat aaatattata
     1561 ataaaagata tgttcttggt aacataaaca aatatttaat taaaataatg cttggcattg
     1621 acgtttatct tataaagtaa gaagatcatg gacatttcaa ccaaaaaagt agagttctcg
     1681 atgaaatcaa aattccttgt acttgcactt tgtgtacctg cgatctttac tacacatgct
     1741 accactaact atccactttt cataccggac aacatcagta cggatattag tctaggatct
     1801 ctgagtggca aaacaaaaga acgcgtttat catcccaagg aaggggggag aaaaattagt
     1861 caactggact ggaaatacag taatgcgact attgttagag gtggcatcga ttggaagcta
     1921 attccaaaag tgtctttcgg agtttccggt tggactactt taggtaacca gaaagcaagc
     1981 atggttgata aagactggaa caattccaat acccctcagg tatggaccga ccagagctgg
     2041 catcccaata cgcatctccg tgatgctaac gaattcgagc tgaatcttaa aggttggtta
     2101 ttaaataatt tggattatcg acttggacta atagcaggtt accaagagag tcgttacagt
     2161 tttaatgcaa tgggagggag ttatatttat agtgaaaacg gggggaacag gaataaaaaa
     2221 ggggcacatc ctagtggtga aagaacaata ggttacaaac agctcttcaa aataccttat
     2281 attggattaa ctgctaatta ccgccatgag aattttgagt ttggagcaga actgaaatat
     2341 agtggttggg ttctttcatc tgatacagat aaacactatc agactgagac aatttttaaa
     2401 gatgaaataa aaaaccaaaa ttactgctct gttgctgcga atattggata ctatgtcacc
     2461 cccagtgcaa aattttatat agaaggctcc agaaattaca tttctaataa aaaaggtgat
     2521 acatctcttt atgagcaaag taccaatata tctggcacca ttaaaaatag tgcaagtatt
     2581 gaatatattg gttttctcac ttccgcaggt ataaagtata ttttttgaat tggttcaccg
     2641 agttgattac gttttcatgg ctaaaaattg ccgcccattg tgagtcatat tcgccctgac
     2701 tttccagaac catacgaact gcccgttgac ggacttcggg ggaaaaacgt gtatttttag
     2761 tcattctgtt tacctctttc tcagggcgtt tagtctccag gatttccggg gcggttcaaa
     2821 aaactcttcg ttgattactg tggacttact gttggcatta ccgaccctga gaccggagaa
     2881 ataagaaccg cttagatcat cgtggctgtt ctcggggtat caagttacac atgggcagag
     2941 gctacccaag gtctactatt atcgcctgaa agcgctgatg gaacagtgct atcagggaca
     3001 tgctgatgaa aatacctaaa taatcagcca gcactctatc tttccaaatc cacagcatag
     3061 caaagagagc aaaagagcct gtaaattcag aaattaagga aacgcctctt ggtgcacata
     3121 atctatctta tgttaaatga aatctaattt cttgattata tgctggatcg ttcgtgggtg
     3181 gctttgcccc tatatttcca gacacctgtt atcattggcc cgctgccgca gatattcccg
     3241 tggcgagcga taatccagcg cactatgcgg atgccattcg ttataatgct cgaatgcctc
     3301 tgcaaggttc tttgctgccg ttaacccgtc aggtttgggc atgatactga tgtagtcaca
     3361 ctttattgtt ttcacgaagc tctttgctat tctgttactc tccggactcc gcaccgccgt
     3421 gttcttcggt tcaacaaagt cccaacatcc gggtgaactg gcgtgtttca ttagcccggt
     3481 agcatgaacc attatccgtc agtcactcca ctggagacga cggaagatcg ttgccgaagc
     3541 ggtgttccac cgctcccagc atgtcgccaa tgggagagca tctacggtct gattgaactg
     3601 tttattcaaa ttgtccaccg tctgtcagta cgctctgaac gtcggctggt taaaacactg
     3661 ctcgctgatt ttcagagggt tcacggtaaa acagcacttt tattccggat tgcggaagcg
     3721 gctctgaata atccggatgg gctggttaaa gaggttgtgt accatctgca tattgttgaa
     3781 ccagatcgca gcggtaagag aagttcatca tacttggcgc agttacgcga tgtttctgct
     3841 agaggtgatg ccgtaaagaa cggcagaaca ttaccggaac aagatagcgg tctacctgca
     3901 ctggtatcag acccggggct tccccggatg atctccacgg tactttaggt actgtgtcta
     3961 tattatgtag gtaggatcaa ttaactttgt agttgaaatt ctttcattga aataatgaat
     4021 aaaaaagaca aaccgcatta aacaatgcca gaatataatt gcatttttgt tccgtttttt
     4081 taaatcatag tatacaaaga agcttgcata ctatatgaaa atataacacc ctttactatg
     4141 atgtttataa aacaatatat ggagtcatgt aggtatgaat ttagatggtg ttagaccata
     4201 ctgtagaata gtcaataaaa agaatgaaag catatcagat attgcatttg cacatataat
     4261 aaaaagggta aaaaattcat catgtactca cccaaaagca gcattggttt ttttaggaga
     4321 gaaaggtttt tgtgatagca atgatgttct atctattatg ggacaacaaa taccaagagt
     4381 atttaagaac aagatgttat atgattatgt ttttaaaaat gaaaaaagta aaaatgattt
     4441 tctaaaaatg gctgaatcat ggctaccaca gagtgaacca atagtaataa ataatgatga
     4501 tgacgcattg aatgctgctg cttatttttc tgtaaaaaaa gcgaaaataa aaacagtaaa
     4561 cgatactgat tttaaagagt ataataaggt ttatattctt gggcacggta gtcctggttc
     4621 tcatcaatta ggccttggtt cggaacttat tgatgtacaa acaatcattt caagaatgaa
     4681 agactgtggt attctaaatg tgaaagatat ccgttttact tcatgcggct ccgctgataa
     4741 agtggctcct aaaaatttta acaatgcccc tgctgaaagt ctttcttgta tccttaactc
     4801 tctgcctttt tttaaggaaa aagaatcttt gctagagcag ataaaaaaac accttgaaaa
     4861 cgatgagtca ttgagtgatg gtctaaaaat atccggctat catggatatg gagttcacta
     4921 tggtcaagag ctttttccct actcacatta tcgttcaact tcaattcctg ctgatccgga
     4981 gcatacagta aaaagaagct ctcagaaaaa gacttttatt attaataaag aactggatta
     5041 gtataaaatt tttaacctat agttaattgt ttgcatttaa gatgaagtta ttttatcttt
     5101 agtagaaaag tgcttcccta tgagggaaaa tcacaaatca tcataataca gagacaaaac
     5161 gatacgaaca aatagataaa aaacatcatt cttttaccac attatttttc gtgaatatga
     5221 aaaataatgt tattacttta atataagact attttttgtt tttccatcac tctgttcaaa
     5281 tttttccgca tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc
     5341 atcaattaat tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta
     5401 ttgctacaaa tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca
     5461 ctcaacaaac ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag
     5521 tagtatttca ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc
     5581 gtgtacgtct cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg
     5641 cttatggcat ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag
     5701 tactacagga tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc
     5761 gtccattcgt tatttataaa gacgcaacct gtacacctga taaagatgag aaaatggcta
     5821 tcactggctc ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg
     5881 cggagattaa tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa
     5941 gtcgggtcat ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag
     6001 catcggttgt tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca
     6061 aaaaagagtt tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag
     6121 cctgtatcag gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt
     6181 ctatgggcac accacgtttt actcctgaat ttaagggatt actggaaagg ctgggacata
     6241 tccctccggc agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa
     6301 cctgagttca cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc
     6361 aatgtggtgt tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa
     6421 tgacaacttc agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt
     6481 gcctgatacc gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat
     6541 tgcattgtca agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac
     6601 caatccatta ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa
     6661 gtattcaaca tcatacagat tacaatatat attgttgttc attttctcca tctctgcgat
     6721 atcttgtttt gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatc
     6781 gcctaaagaa taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat
     6841 ctcttcataa ttagatgctt taat
//
LOCUS       DADLTI010000186         6849 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLTI010000186 DADLTI010000000
VERSION     DADLTI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.188"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..893)
                     /locus_tag="JN755_003655"
     CDS             complement(<1..893)
                     /locus_tag="JN755_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7382296.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="JN755_003656"
     CDS             953..1231
                     /locus_tag="JN755_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382297.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JN755_003657"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JN755_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7382298.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JN755_003658"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JN755_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7382299.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="JN755_003659"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="JN755_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7382300.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="JN755_003660"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="JN755_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7382301.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="JN755_003661"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="JN755_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="JN755_003662"
     CDS             complement(4911..5441)
                     /locus_tag="JN755_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7382302.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JN755_003663"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JN755_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7382303.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6410..>6849)
                     /locus_tag="JN755_003664"
     CDS             complement(6410..>6849)
                     /locus_tag="JN755_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382304.1"
                     /translation="LHKYEQHLDKQINGLFSTLLTINDNSLRAKAEIASRIIDFLEAK
                     VVNLSFDDILKYKQEFKKICYKQLQEFTTPSLYNKIQKWATMSKNEFIAFHYETLQPE
                     KISYPFYLKRDLPNEKDINYGVEIEIPSGKRIRLSNHYQNIIP"
BASE COUNT         1994 a         1388 c         1450 g         2017 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttggtag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt
     6841 tatgtagtt
//
LOCUS       DADLTI010000187         6803 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLTI010000187 DADLTI010000000
VERSION     DADLTI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.189"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            95..1519
                     /gene="sbcB"
                     /locus_tag="JN755_003665"
     CDS             95..1519
                     /gene="sbcB"
                     /locus_tag="JN755_003665"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7382305.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1562..1789)
                     /locus_tag="JN755_003666"
     CDS             complement(1562..1789)
                     /locus_tag="JN755_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7382306.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1803..2861)
                     /gene="yeeE"
                     /locus_tag="JN755_003667"
     CDS             complement(1803..2861)
                     /gene="yeeE"
                     /locus_tag="JN755_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7382307.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3040..4398)
                     /gene="plaP"
                     /locus_tag="JN755_003668"
     CDS             complement(3040..4398)
                     /gene="plaP"
                     /locus_tag="JN755_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7382308.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4388..4468)
                     /gene="yoeI"
                     /locus_tag="JN755_003669"
     CDS             complement(4388..4468)
                     /gene="yoeI"
                     /locus_tag="JN755_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7382309.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4666..5595)
                     /locus_tag="JN755_003670"
     CDS             complement(4666..5595)
                     /locus_tag="JN755_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7382310.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5641..6465)
                     /locus_tag="JN755_003671"
     CDS             complement(5641..6465)
                     /locus_tag="JN755_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7382311.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6548..6802)
                     /gene="yoeB"
                     /locus_tag="JN755_003672"
     CDS             complement(6548..6802)
                     /gene="yoeB"
                     /locus_tag="JN755_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7382312.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1840 a         1782 c         1595 g         1586 t
ORIGIN      
        1 atatttgacg ttcgtagaca tactcaggtg aaaaaagggt tgcttttcga gttataacag
       61 acagttaatt aactacagtg gacaatgcta gacaatgaca gacacagata aacaacctac
      121 cttcctcttt cacgattacg aaacctttgg cacgcacccc gcgttagatc gccctgcaca
      181 gttcgcagcc attcgcaccg atgacgaatt caatgtcatc ggcgaacccg aagtctttta
      241 ctgcaagccc gcggatgact atttaccccg gcctggagca gtattaatta ccggtattac
      301 cccgcaggag gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc gtattcactc
      361 gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt tcgacgacga
      421 agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga gctggcagca
      481 tgataactcg cgctgggatt tactggatgt tatgcgtgcc tgttatgccc tgcgcccgga
      541 aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg agcatttaac
      601 caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg tgtacgccac
      661 tattgcgatg gcgaaactgg taaaaacgcg tcagccacgc ctgtttgatt atctctttac
      721 ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga aacccctggc
      781 tcacgtttcc ggaatgtttg gggcatggcg tggcaatacc agctgggtgg caccgctggc
      841 gtggcatcct gaaaatcgca atgccgtaat tatggcggat ttggcaggag acatttcgcc
      901 attactggag ctggatatcg acacattgcg cgagcgttta tataccgcaa aagccgatct
      961 tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc cggtactggc
     1021 ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc gtcagcattg
     1081 cctcgataac ctaaaaattc tgcgtgaaaa tccgcaagtg cgtgaaaaag tggtagcgat
     1141 attcgcggaa gccgaaccgt ttacaccttc agataatgtg gatgcacagc tttataatgg
     1201 ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa
     1261 tttaccggca ctggatatca cttttgttga taaacgaatt gaaaagttgt tgttcaatta
     1321 tcgggcacgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct ggcaggagca
     1381 ccgccaccag gtcttcacgc cagagttttt gcagggttat gctgatgaat tgcagatgct
     1441 ggtacaacaa tatgccgatg acaaagagaa agtggcgctg ttaaaagcac tttggcagta
     1501 cgcggaagag atcgtctaat ttgaagccgc cgccgctggt acggcggagg acaatgttga
     1561 gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc aatctgctga taatcggtga
     1621 tggcatgtcc ttcttctgcc gcccactgtg ggattgcttc agttgcctgg gtacaatcaa
     1681 actcaattac caactcatcg ccgctggcca tttctgccag tgctgctttt gcttctatga
     1741 gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc tttacacctt
     1801 acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac atacacaagc
     1861 caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc ctgccaggtc
     1921 atcattgcag tcataaccag tccattaccg atagagcaac cacctgcaat actggcaccg
     1981 aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc agctgcgcga
     2041 acgcggaact cacggctcgc tttggctgca ataaatgccc ccacgaagat ccctaacacc
     2101 aggaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg cagaatattg
     2161 gccgttgggg aagtgactcc taacccaaac atgcgtccgg ttgcttcact caggggccag
     2221 gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca gcgtttttca
     2281 aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc
     2341 ttcttcaact ctttcatcac cacccagagc gttatcacca gcaaaacggt aaccaacggc
     2401 cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtagtgta gtgctgcaag
     2461 gtttgattta aaccactggc gtgtggagaa cgcatcaccg cactcatcac catataagtg
     2521 aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt cccggtggca
     2581 catccacccg ccagaacaat tcccagcccg aaaagatacc cacctataac agtaccaagc
     2641 cacgggaacg ccccggcttc gtaagtcagt aggcccgcca gaattaacgc aaaaaccccc
     2701 acgctttgtg ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt tttcgcgata
     2761 tacatatcgc gaaaaccacc agtcaggcaa aaacgcccac gctgcatgac aaatcccagc
     2821 agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt aaccagatga
     2881 atgttaatgg ggaaattttt catgactggt ggaatgcaga ccaataacca aattctctaa
     2941 attagaacaa atggttatta atgaggattt cgataaaaaa gaataaaaaa accggagcca
     3001 aatgttcatt cgactccggt ttattattag aaaagatgat tatgctacgt cttcgtactg
     3061 cggaaccgga ttacggaagc tcttggtgac gcaagccagg taaatcagac cgatagcagc
     3121 ccagatcaga cccagaacca ttgagctttc ttccaggtta acccacagcg caccaacggt
     3181 cagcgcacca cacatcggca ggaacagata ctggaagtga tctttcagcg tcttgttacg
     3241 cttctcacgg atccagaact gcgagatgac cgacaggtta acaaaggtga acgccaccag
     3301 cgcaccaaag ttaatcagcg ccgttgccat taccaggtcg aagttgattg ccagcagggc
     3361 aatcgcgcca accaggatga tgttcatcgc cggagtacgc catttcgggt gtacataacc
     3421 gaagaagctt ttcgggaaca cgccgtcacg acccattacg tacatcagac gcgctacgcc
     3481 tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg gagaagatca gcgcgccaac
     3541 ctggaacgct ttacccgcaa cgtacagcat gatttcaggc tgtgacgcat ccggatcttt
     3601 aaagcgagag atatccggga agtacagctg caggaagtaa gttgcaaaga tgaagatcat
     3661 gccgccaatc agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg catctttggt
     3721 ttcttccgac agattgctga tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc
     3781 cccggtaatc atcgggataa catgcgcatc gccagaccag aacggacgcg tgctcgccag
     3841 cgtaccagcg ccttcacctt caaatacgcc ataaacaacc atgcccagaa tcaccgcgat
     3901 cagcactacc tgcaacacga cgattacagt gttgaagttc gctacggatt tcagactacg
     3961 caggttaaag gcggtcataa aggccaccag cgccaccaca aacatccacg atggaatgga
     4021 aggcaccaga gcttcaaaat agattttcgc cagcagaatg ttgatcatcg gcgcgaacag
     4081 atagtcgagc agagaagacc aacccaccat aaagccgaca gtcgggctaa tggatttctg
     4141 ggcgtaagtg tatgcagagc cagcagaagg atagcggcga accagcttcc cgtagctcag
     4201 agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat gaccatccgt
     4261 gaggcctgaa acgataccaa atgtatcaaa cagcgtcatc ggctgcatat aggctagacc
     4321 catcattaca accggaacta acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg
     4381 agtaacgtta tgcgacatgg ttattctcct ttacggtgat aaccgtcgcg taagcaaaaa
     4441 atttgcccca tttttttgga ttcctcagcg acaacaactg tcgattttta gtaaatatct
     4501 atccggtacg aagcccggcc tcttggtatg aatgattggt ttgaagcaaa aaataaccga
     4561 cgctaattaa acgtcggttt ttagtcattt tttgacagcg gcgcattgtg cctaaatggg
     4621 ggggaaatga caagagaatg agaggcttgt cataataatt tttctttaaa tggctgattt
     4681 tccgtcatca gattttgcat aaacaccggc gatcgcacta tttgctaaaa tttcatctcg
     4741 ccaccagccg ctggcaagcc ctgcggtctg ttcattccag ccaatccaga caggttcaaa
     4801 gagagtttgg gcgaccactt tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc
     4861 cagataccgt ggtaaatagc cgcagccaag gccgctaatt tgcagctcca gtttggtttt
     4921 aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc
     4981 tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt ttaatgatac gacggtttaa
     5041 cggttcttct tcctgggcta acggatgatg aggcgcgacc gcaaagactt gttcaagatc
     5101 gcccaaccgc gagaaaccaa actcactgga agatggtggc tcatgcatcg cgccaacgat
     5161 aatatccgct cgcccctgag tcagggcatc ccaggaacca ccgagtacgc cattgataaa
     5221 cttcaggcgc gtcacgctat gatgttgata aaaagcttca atgagaggcg caagcagaga
     5281 aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt tcccagcctt catgcagttt
     5341 gatcgcctgc ttttccagtt ctcgcacggt atgcagaact tcccgccctt tctctaataa
     5401 catttttccg gtgcgggtga atttagcgcg gtgtccgcta cgatcaagca attgaatatt
     5461 taggtcgctt tccagcttgt gaacggtgta actcagagcg gacggcgtct tgtagagttt
     5521 ggctgacgcc gccgcaaaac tgccctcttt ttctatggca tcgaggatca tcaacacatc
     5581 cagcagtggt ttcatacacg cccccttgca gtgcatgcgg cgtcctgctg gcgtgatcgt
     5641 ttactccagc ggcattacca gcggatcggg atactggtat tcaaatccca gttcattaca
     5701 aatccgactg ccatcaataa tcttaccttt gccgctgtcc agactatttc tgaactgcgg
     5761 aggttgcagc cccagtaaac gggccatctg cggatagaaa acattacgcg cagggtgagc
     5821 tggcgcacat atattataga tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc
     5881 gccaataaca tcttctaaat ggactaaatt aacaccatgt tcaccatcag gggcggtttt
     5941 tccggcaaag aagcgtcccg gatgacgtcc cggtccgacc aggcccgcaa gacgcagaat
     6001 atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg agttcttcta atactcgtcc
     6061 actgttggtt accggattac gcggggtagt ttctttaacc gtgccttgcg cgtcgccata
     6121 gacagatgtt gagctggtaa aaataattcg gggaatacga tgggccagcg cgctatccac
     6181 taactcttgt accgcttgta aatagaactc atcgccgggg ccgctacgac gtgccggaag
     6241 cgtaatgacc agcgcatcgg catccataag ggcatccaga tcgtcagaat cgcaaactaa
     6301 ctcaggctcc atgcgaagca gatagctatc aatgccactc attcgggccg cttcgacacc
     6361 atcttgtgtg gttttactcc cggtgacttg ccagcctcgc gctgaaagtg acatcgccag
     6421 cggcatacct aaccacccta acccgacaat tgcgaccttt ttcatccgtt atctcctgat
     6481 acaccttaga tctataaggc tacgctagcg tatcaaaact gacaattcat tctatgaatg
     6541 aatctgttca ataatgataa cgacatgctg caatgagcag tgaatcatca gtaaccgcgt
     6601 ataccagacg gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca
     6661 ggggttctgg tttcccctta ccttcaaatg gtgttctgcg ggtatctttg ataagttcat
     6721 tgatcttttt aacaattcgc ttatctgttt cctgccagta cagataatca tcccatgatt
     6781 cctcagacca gattagtttc act
//
LOCUS       DADLTI010000188         6791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLTI010000188 DADLTI010000000
VERSION     DADLTI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.190"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            239..2671
                     /locus_tag="JN755_003673"
     CDS             239..2671
                     /locus_tag="JN755_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7382313.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4198
                     /locus_tag="JN755_003674"
     CDS             2702..4198
                     /locus_tag="JN755_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY7382314.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4207..5868
                     /locus_tag="JN755_003675"
     CDS             4207..5868
                     /locus_tag="JN755_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY7382315.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JN755_003676"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JN755_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY7382316.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6791)
                     /locus_tag="JN755_003677"
     CDS             complement(6477..>6791)
                     /locus_tag="JN755_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382317.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         2003 a         1613 c         1549 g         1626 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga a
//
LOCUS       DADLTI010000189         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLTI010000189 DADLTI010000000
VERSION     DADLTI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.191"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..3524
                     /locus_tag="JN755_003678"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JN755_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JN755_003679"
     CDS             3534..6749
                     /locus_tag="JN755_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY7382318.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQ
                     RLIWGVIRQMNRVVYSTPFVWPQRGSSAMRKMSQLTCLRTL"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADLTI010000190         6715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLTI010000190 DADLTI010000000
VERSION     DADLTI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.192"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..766
                     /locus_tag="JN755_003680"
     CDS             <1..766
                     /locus_tag="JN755_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7382319.1"
                     /translation="NDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            777..1850
                     /locus_tag="JN755_003681"
     CDS             777..1850
                     /locus_tag="JN755_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382320.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2098..3138
                     /gene="pstS"
                     /locus_tag="JN755_003682"
     CDS             2098..3138
                     /gene="pstS"
                     /locus_tag="JN755_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7382321.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3225..4184
                     /gene="pstC"
                     /locus_tag="JN755_003683"
     CDS             3225..4184
                     /gene="pstC"
                     /locus_tag="JN755_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7382322.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4184..5074
                     /gene="pstA"
                     /locus_tag="JN755_003684"
     CDS             4184..5074
                     /gene="pstA"
                     /locus_tag="JN755_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7382323.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5166..5939
                     /gene="pstB"
                     /locus_tag="JN755_003685"
     CDS             5166..5939
                     /gene="pstB"
                     /locus_tag="JN755_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7382324.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5954..6679
                     /gene="phoU"
                     /locus_tag="JN755_003686"
     CDS             5954..6679
                     /gene="phoU"
                     /locus_tag="JN755_003686"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7382325.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1697 a         1572 c         1746 g         1700 t
ORIGIN      
        1 aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc tggaaagaaa
       61 taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg ttggcggtaa
      121 tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata actacagcca
      181 aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca gcgaaggggt
      241 aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg gggccgccgg
      301 gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata cggtcaagac
      361 acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt tctcgaagtc
      421 gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg cagtggttaa
      481 agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa atgcaaatgg
      541 taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg cagattcagg
      601 aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag gacaggttac
      661 gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcac tttcgacacc
      721 agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg atttttatga
      781 acaagtatat caaacagtgg tgcttttctg tgtttatgct ctcgttaagt agtgtagcac
      841 ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt tcgcttgatt
      901 tctcgggata tcttattacc gcgaacgaaa ataaggttgg aacgacattt aacaccactg
      961 taactaatgg atcctcatat cttggacgct gtcattgtga tactggtaac gtaggagaat
     1021 tcccctatat ctattatacg tcaaaaataa accaggcatt aacttatgct ggggttcact
     1081 ctaatattaa ttattatgat cttaatccga accttgatgt tgggattgct atagatattc
     1141 ttggggttgg gtatgtgaat gcaccttttg aatatcatgc taacaacccg tctggtaata
     1201 caaagtacaa ttgcaaccgc attgaacctt taagtatatc cagtggggct aaagcgatag
     1261 tatattttta tatcaagaag acatttgcag gaaaattgat tattcctgaa acaaagatag
     1321 tgacattgta tggaacaatt agccgtgaca ccccggtgga ttactcacag ccgatggctg
     1381 atgtttatat tcggggcgat attacagctc cgcaaagttg cgaaataaat aatttaaagc
     1441 cagtttgttt tgattttaaa gaaataccag ctgcagattt ttcatctgtt gttggaagtg
     1501 cggtaacaac acataaaatt actaaaacag tcactattga gtgtgaaaac ctagggatac
     1561 tgaataccga tgatatcagt acgtcttttt atgctaccga acccaatacg gacaattcaa
     1621 tggtcgtaac ttcaaactct aacgtgggga taaaaattta cgacaaaaat aataaggaaa
     1681 tcaaagtgaa cggtggtgag ttgccaacag acatggataa atcaacagtg tatggtgaaa
     1741 aatcaggtag tgtaactttt tcagctgctc ctgcgagcct tactggagct cgcccagccc
     1801 cggggcaatt tactgcaaca gcgacgataa ctgttgagat tgtgcgctaa taggtctgac
     1861 aacgattgtt catcacatcg ttgtcatctc acccatttct ttatcacttt taaatgcatc
     1921 atgacaaaaa gatggcatgt gggattttca tcttcacatt taaaatcagt aagttatttg
     1981 gtaattcctt tctgtcataa aaatgtcata ttccttatat ataactgtca cctgtttgtc
     2041 ctattttgct tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg
     2101 aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc
     2161 tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat
     2221 gccaaatggg ctgacactta ccagaaagaa accggtaata aagttaacta ccagggtatc
     2281 ggttcttccg gtggcgtaaa acagattatc gctaataccg ttgattttgg tgcctctgac
     2341 gcgccgctgt ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt
     2401 ggcggcgtgg tgctggcggt taacattcca gggctgaagt ctggcgaact ggtgctggat
     2461 ggtaaaaccc tcggcgacat ctacctgggc aaaatcaaga agtgggatga tgaagccatc
     2521 gccaaactga atccgggtct gaaactgcct tcacaaaaca ttgctgtagt acgccgcgca
     2581 gatggctccg ggacttcctt cgtcttcacc agctacctgg agaaagtgaa cgaagagtgg
     2641 aaaaacaacg ttggtactgg ctctaccgta aaatggccga tcggtctggg cggtaaaggt
     2701 aacgacggta tcgccgcgtt cgttcagcgt ctgccgggtg caattggtta tgttgaatat
     2761 gcttacgcga agcagaacaa cctggcgtac accaaactga tctccgctga tggtaaaccg
     2821 gttagtccga ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc
     2881 ttcgctcagg atctgaccaa ccagaaaggc gaagatgcat ggcctattac ctctaccacg
     2941 ttcattctga tccacaaaga tcagaagaaa ccagaacaag gcacagaggt gctgaaattc
     3001 ttcgactggg cgtacaaaac cggggctaaa caggcgaacg acctggatta cgctagcctg
     3061 ccggatagtg tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc
     3121 ggtaagccgc tgtactaata aaactccagg ccgggtacgg tgttttacgc cgcatccggc
     3181 attacaaaat gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc
     3241 ccgcttttaa cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg
     3301 cggcgctgat tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcct
     3361 ggccgagcat tcagaaattt ggtctggctt tcctatggac caaagagtgg gatgcaccga
     3421 acgatatcta cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc
     3481 tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg
     3541 gctggctgaa acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg
     3601 tttacggcat gtggggcctg tttatctttg cgccgctgtt cgccgtttac tttcaggagc
     3661 cggtcggcaa tatcatgtcg aatatcccga ttgttggcgc gctgttctct ggccccgcat
     3721 ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg
     3781 cggcggtaat gcgtgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg
     3841 gtattggctg caccacctgg gaagttatct ggcgtatcgt tcttccgttc accaaaaatg
     3901 gtgttatcgg cggcatcatg ctggggctgg gccgcgcgct cggtgaaacc atggcggtga
     3961 cctttatcat cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaata
     4021 gtatcacctc tgcgctggcg aacgaatttg cggaagcgga atccggtctg cacgttgccg
     4081 cactgatgga actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga
     4141 agtttatgat tatgcgcctg gctaagaatg agggggcacg ctaatggcta tggttgaaat
     4201 gcaaaccact gcggcgctgg ctgaatctcg ccgcaaaatg caggcgcgtc gccgcctcaa
     4261 aaaccgtatt gcgctgacgc tctcgatggc gacgatggcc ttcggcctgt tctggctgat
     4321 ctggatttta atgtccacca tcactcgcgg tatcgacggt atgtcgctgg cgctgttcac
     4381 tgaaatgacg ccgccgccca atacggaagg tggtggtctg gcgaacgccc tggcgggtag
     4441 cgggctgtta attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat
     4501 ttatctggcg gaatatggtc gaaaatcctg gctggcagaa gtgattcgct tcattaacga
     4561 cattctgctc tccgcgccgt cgattgtggt tggtctgttt gtctacacca tcgtggtggc
     4621 acagatggag cacttctccg gctgggcggg cgtaattgcg ctggcgctgt tgcaggtgcc
     4681 gatcgttatc cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc
     4741 ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc
     4801 ggtgtccggg attatgaccg gtatcctgct ggcgattgcc cgtattgccg gtgaaaccgc
     4861 gccgctgctg tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat
     4921 cgccaacctg ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcaaca
     4981 actggcctgg gccggggtat tgatcattac cctgtgcgtc ctgctgctga acattctggc
     5041 gcgcgttgtt tttgcgaaga ataaacacgg ttaacacttt gccggatgcg gcgtgaacgc
     5101 cttacccggc cctacggtaa gcctgattag cgaaaagcat caggtacgat gaggaaaaga
     5161 ttgcaatgag tatggttgaa actgccccga gtaaaattca ggttcgtaat ttgaacttct
     5221 actacggcaa attccatgcc ctgaaaaaca tcaacctgga tatcgccaaa aaccaggtta
     5281 cggcgtttat cgggccgtcc ggctgcggta aatcgacgct gctgcgtacc ttcaacaaaa
     5341 tgtttgaact gtacccggag ctgcgtgcgg aaggtgaaat tctgcttgat ggcgacaaca
     5401 tcctgaccaa ctctcaggat atcgccctgc tacgtgcgaa agtgggcatg gtgttccaga
     5461 aaccgacgcc gttcccgatg tccatctacg acaacatcgc ctttggcgtt cgtctgtttg
     5521 agaagctctc ccgtgccgac atggacgagc gcgtgcagtg ggcattgacc aaagccgcat
     5581 tgtggaacga aaccaaagat aaattgcacc agagcggtta ctctctctct ggtggtcagc
     5641 aacagcgtct gtgtattgcg cgtggtatcg ccattcgccc ggaagtgctg ctgctcgacg
     5701 aaccgtgttc ggcgctcgac cccatctcta ccgggcgtat tgaagagctg atcaccgaac
     5761 tgaagcagga ttacaccgtg gtgatcgtta cccacaacat gcagcaggct gcgcgttgtt
     5821 ccgaccacac ggcatttatg tacctgggcg aattgattga gttcagcaac acggacgatc
     5881 tgttcaccaa gccagcgaag aaacaaacag aagactacat caccggtcgt tacggttgat
     5941 tcaggagtgc gttatggaca gtctcaatct taataaacat atttccggcc agttcaacgc
     6001 cgaactggaa agtatccgca cgcaggtgat gaccatgggc ggcatggtgg agcagcagct
     6061 ttctgatgca atcaccgcga tgcataacca ggacagcgat ctggcgaagc gcgtgatcga
     6121 aggcgacaag aacgtcaaca tgatggaagt ggcgatcgat gaagcctgcg tgcgcattat
     6181 cgccaaacgt cagccgacgg cgagcgacct gcgactggtt atggtgatca gtaaaaccat
     6241 tgccgagctg gagcgtattg gcgacgtggc ggacaaaatc tgccgtactg cgctggagaa
     6301 attctcccag cagcatcagc cgttgctggt aagtctggag tcgctgggcc gtcataccat
     6361 ccagatgctg cacgacgtgc tggacgcgtt cgcgcggatg gacattgacg aagcggtacg
     6421 tatttatcgt gaagataaaa aagtcgatca ggaatacgaa ggtattgttc gtcaactgat
     6481 gacctacatg atggaagatt cgcgcactat tccgagcgta cttaccgcgc tgttctgtgc
     6541 gcgttcaatc gaacgtatcg gcgaccgctg ccagaatatt tgtgagttta tcttctacta
     6601 cgtgaagggt caggatttcc gtcatgtagg cagtgatgag ctggataaac tgctggcggg
     6661 aaaagatagc gacaaataat tcaccagaca aatcccaata acttaattat tggga
//
LOCUS       DADLTI010000191         6697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLTI010000191 DADLTI010000000
VERSION     DADLTI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.193"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JN755_003687"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JN755_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7382326.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JN755_003688"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JN755_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7382327.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JN755_003689"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JN755_003689"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7382328.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JN755_003690"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JN755_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7382329.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JN755_003691"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JN755_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY7382330.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JN755_003692"
     CDS             4025..4615
                     /locus_tag="JN755_003692"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7382331.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JN755_003693"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JN755_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7382332.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JN755_003694"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JN755_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7382333.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JN755_003695"
     CDS             complement(6551..6658)
                     /locus_tag="JN755_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382334.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
BASE COUNT         1429 a         1803 c         1839 g         1626 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcgacta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacacca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacacgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggctt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg tccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgt gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcttg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat
     6301 ggtcgcagta accttcgttc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatag
//
LOCUS       DADLTI010000192         6675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLTI010000192 DADLTI010000000
VERSION     DADLTI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.194"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..802)
                     /gene="mutY"
                     /locus_tag="JN755_003696"
     CDS             complement(<1..802)
                     /gene="mutY"
                     /locus_tag="JN755_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7382335.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADE"
     gene            963..1682
                     /gene="trmB"
                     /locus_tag="JN755_003697"
     CDS             963..1682
                     /gene="trmB"
                     /locus_tag="JN755_003697"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7382336.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            1682..2008
                     /locus_tag="JN755_003698"
     CDS             1682..2008
                     /locus_tag="JN755_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7382337.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            2192..2911
                     /locus_tag="JN755_003699"
     CDS             2192..2911
                     /locus_tag="JN755_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7382338.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            3087..4133
                     /gene="ansB"
                     /locus_tag="JN755_003700"
     CDS             3087..4133
                     /gene="ansB"
                     /locus_tag="JN755_003700"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7382339.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            4250..5257
                     /locus_tag="JN755_003701"
     CDS             4250..5257
                     /locus_tag="JN755_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7382340.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(5313..6614)
                     /locus_tag="JN755_003702"
                     /pseudo
     CDS             complement(5313..6614)
                     /locus_tag="JN755_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         1888 a         1582 c         1712 g         1493 t
ORIGIN      
        1 cttcgtcggc aaacttcggg aaacagtata aaccgcccca caatccgctc ggcggacgct
       61 gcgccagcaa tacttcatct tcgtgctgta acaggagaaa gtagccggtg cgctccggca
      121 gcatctgttt cggttttttg cccggataaa gcgaccagct attgttggtg gcggcaatac
      181 atccgttttg tagcggacag agcgaacatt tcggcttcga acgcgtgcaa atcatcgcgc
      241 ccaaatccat catcgcctga ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc
      301 tcaaactcca tagtttattc tcgacctctt ttttcccagg ccagccgctt acagcatagc
      361 agcgcgccag cacgcgtttg acgttaccgt cgagaatcgg aaagtgcttg cccagagaaa
      421 gcgagagaat cgcgcctgcg gtggaacgcc cgacgcccgg taacggcgcg acttcttcaa
      481 aggtttccgg gaatttacca ccgtgtaagg tcgccacttg ttgtgccgct ttatgcaggt
      541 tgcgcgcgcg ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg
      601 cattggcgag atcggtcacc gtcgggaagt gcgccataaa gcgttcaaaa taggggataa
      661 cggtcgcaac ctgagtttgt tgcaacatca cttctgagag ccatactttg tagggcgtct
      721 tgtcaatttg ccagggcagc gttttccgcc cgtatttatc gtaccagtcc agaacctggg
      781 ctgaaaattg cgacgcttgc atggtcaccg acttcactgt tgttgggggc aagattgcag
      841 cacagcgacg gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca
      901 tccggcaact aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaatct
      961 ttatgaaaaa cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta
     1021 tccgtagttt tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa
     1081 attactggcc ggtgatgggc gttgagttca gtgaagatat gctggatttc cccgcgcttt
     1141 ttggccgtga agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg
     1201 caatggctaa agatcgcccc gagcaggact tcctcggcat tgaagtgcat tcaccgggcg
     1261 ttggtgcgtg cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc
     1321 acgatgcggt tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc
     1381 tctttttccc tgacccgtgg cacaaagcgc gccataataa acgccgtatc gttcaggtgc
     1441 cgtttgccga actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg
     1501 actgggaacc ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa
     1561 acctgtcaga gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg
     1621 aacaacgtgg tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat
     1681 aatggcaaag aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga
     1741 attaggattt tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa
     1801 aatcgttgat gactttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag
     1861 cggttatctg gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga
     1921 acatcaggcg attgtgcgta agtggctgga agagtgcaaa ctggatgagg tacgcaccag
     1981 cgaacttttc gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact
     2041 gcctgatgcg ctacgcttat caggcctaca gagatgcacg atcgagtagg cgggataagg
     2101 cgtttacgcc gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcata
     2161 tgctggcctg atttgttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc
     2221 actttcagtg acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga
     2281 cgatgtgatt gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat
     2341 cacgccagac ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg
     2401 cgagcaggcg aaggattatc aggctgaact acgtagcacg ctgccgtgga ttgatgaagg
     2461 cgcgaaaagc cgcgtcgaga aagcccgtat tgcgctggat aaaattatcg ttcaggagat
     2521 gggcgacagc agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc ttaaagagca
     2581 gatgaatcgc attatcgaaa cgcgcagcga tggcctgacg ttccactata aagccattga
     2641 tcaggttcgc gccgaaggcc agcaactagt gaatcaggca atgggcggaa ttttacagga
     2701 cagcattaat gaaatgggcg cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa
     2761 tgtgctggga agcttgggcg gtctgcaatc ctcaatccaa accgagtgga aaaagcagga
     2821 aaaagatttc cagcagtttg gcaaagatgt ttgtagccgc gttgtgactc tggaagatag
     2881 ccgcaaagcc ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt
     2941 tttatgccgt ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata
     3001 tattcacgtt gtaaattgtt taacgtcaaa tttcccatac aaagctaagg gataatgcgt
     3061 agcgttcacg taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac
     3121 tggttatggg ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg
     3181 gcgggaccat tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag
     3241 ttggcgtaga aaatctggtt aatgcggtgc cgcaactaaa agacattgcg aacgttaaag
     3301 gcgagcaggt agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg
     3361 cgaaaaaaat taacgccgac tgcgataaaa ccgacggctt cgtcattacc cacggtaccg
     3421 acacgatgga agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg
     3481 tgatggtcgg cgcaatgcgc ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt
     3541 ataacgcggt agtgaccgca gctgataaag cctccgctaa tcgtggcgtg ctggtggtga
     3601 tgaacgacac cgtactggac ggtcgcgatg tcaccaaaac caacaccacc gacgtagcga
     3661 ccttcaagtc tgttaactac ggtcctctgg gatacattca caacggtaag attgactacc
     3721 aacgtacccc ggcacgtaag cacaccagcg atacgccatt cgatgtctct aagctgaatg
     3781 agctgccgaa agtcggcatc gtttataact acgctaacgc atccgatctt ccggctaaag
     3841 cactggtaga tgcgggctat gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt
     3901 ataaatccgt gttcgacacc ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt
     3961 cttcccgcgt accgacgggt gctaccactc aggatgctga agtggatgat gcgaaatacg
     4021 gcttcgtcgc ctctggcacg ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc
     4081 tgacgcaaac caaagatccg cagcagatcc agcagatttt caatcagtac taatcacctc
     4141 gtcccggcat cgtgccgggg ctttttcact tcagacttac gtccattgcc aattttcatt
     4201 accctaatga taatcaccgg aataaattat tccgcgcgag ggttttcggg tgaaaaagca
     4261 atggattgtt ggcacggcgc tgcttatgtt gatgactggt aatgcctggg cagatggcga
     4321 accgccaact gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa
     4381 gcttgatgcc atcagcttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc
     4441 ggaaggcgat gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta
     4501 cgaactgctc gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac
     4561 cagtaaagga acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc
     4621 cagcgatcgt gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa
     4681 tagcgaagat ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa
     4741 ctcgattcct gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca
     4801 gaaagcagcg gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga
     4861 agcctttaaa aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc
     4921 gttcgcggtt aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca
     4981 gagtgaagag tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca
     5041 acggcgacag acttttctgc aatcacaaaa actcagccgt aaattgcagg atgactgggt
     5101 aacgctcgaa aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa
     5161 agtcgcgatt ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga
     5221 taccgaacaa ctgcggcgca atggggtgat taagtaactc atgttaagtg cctttgccgg
     5281 ataatataaa aagttatccg gcaaattatt ttttacatta gccctagtag ctgtggaatg
     5341 aacaaggata tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa
     5401 aatggcagca atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca
     5461 aatagcgcac tacccaccgg cagtgttaac agcccaatac aaaggttggc caccatcata
     5521 ataccgaaat gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta
     5581 aagattagta cagccggtgt catgtccata aagatgccga caattaataa gacgatatta
     5641 atcagcagca atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata
     5701 taaggaatat cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt
     5761 aatacaatcg aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt
     5821 acttcccggt aaatcagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct
     5881 tcagttgcgg taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa
     5941 cttggtaagg catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagg
     6001 gggtagccac ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag
     6061 atccctggca aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg
     6121 gaaaacacaa ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg
     6181 tttacagcgg tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg
     6241 ccaccaacgg cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc
     6301 gccaggacgt tcacatgacc aagcgaacca ggaacgcggc caaccattac ctaggcgagg
     6361 ttaatcaaac gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag
     6421 gggattgcca acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt
     6481 gccgcgatat cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag
     6541 aacgaaatgg gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg
     6601 atataactat ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat
     6661 taataaaaca caagg
//
LOCUS       DADLTI010000193         6611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLTI010000193 DADLTI010000000
VERSION     DADLTI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.195"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            637..2220
                     /locus_tag="JN755_003703"
     CDS             637..2220
                     /locus_tag="JN755_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7382341.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2672..4525)
                     /gene="asmA"
                     /locus_tag="JN755_003704"
     CDS             complement(2672..4525)
                     /gene="asmA"
                     /locus_tag="JN755_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7382342.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4547..5128)
                     /gene="dcd"
                     /locus_tag="JN755_003705"
     CDS             complement(4547..5128)
                     /gene="dcd"
                     /locus_tag="JN755_003705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7382343.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5220..5861)
                     /gene="udk"
                     /locus_tag="JN755_003706"
     CDS             complement(5220..5861)
                     /gene="udk"
                     /locus_tag="JN755_003706"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7382344.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6179..>6611
                     /locus_tag="JN755_003707"
     CDS             6179..>6611
                     /locus_tag="JN755_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382345.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGAPIK"
BASE COUNT         1549 a         1719 c         1660 g         1683 t
ORIGIN      
        1 agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc ttgcagttaa
       61 ttgaataaaa atttaactgg catcagtcct aaaaaaattg atttcatccg caggctattg
      121 acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc tggcttttta
      181 gctaccaata cactgattga gcttaatttt tcaaaccctc tctgcataca atcgttgatg
      241 agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg taattgaata
      301 ttggctcgcc aataatgcag gaggaagtgt tacagctaac ggaatagcag gcaaggtaac
      361 aattcggcaa ttggctattt ttaagaatta tattaagtgt cattcaatat ggtttttagg
      421 agtttcttta ggttgacaat atttaatatt gcgtctccac atgcgatatt tcttaaataa
      481 tgttttatta ttaccgcttt taattcaggg ataatgtgag gttattaccc tcaaataata
      541 tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg
      601 acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct
      661 atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg
      721 gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt
      781 accgggctgc tgctggcgat gttaatgcgc ctgttactgc tagcgtcaat ctcctggctg
      841 gtcaccctga ctcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgactta
      901 atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg
      961 ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt ctggggcgtg
     1021 gtgacgcaaa ttgtggtact ggacgccatt ttctcactcg actcagtgat taccgccgtc
     1081 gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg
     1141 ttgatggcca gcaagccgtt aacgcaattt gttaacagtc acccgaccat cgttattctc
     1201 tgcttaagct tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc
     1261 attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcactcaat
     1321 cagttggcta tctttaaccg gcgacgtttt ctttccgcta atcagacgct acgccagcgg
     1381 actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc
     1441 gaaaccgcgt ccatgctggt ggatcatgga aaccagcaga tctttaatcc gcaggaacgg
     1501 cggatgattg agcgggtact taatcttaac cagcgtaccg tcagcagcat tatgacgtcg
     1561 cgccacgata ttgagcatat cgatctcaac gcgccggaag aagaaatccg ccagttgctg
     1621 gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga agatttgctc
     1681 ggtgttgttc acgttatcga cctgctacaa cagtcactgc gcggcgaacc gctcaacctg
     1741 cgggtgttga ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt accagccctg
     1801 gagcagttcc gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt tggctcggtg
     1861 gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa
     1921 gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg
     1981 aatggtcata tgccgctgga ggatctggtg caatatgtgc cgctgccgct ggatgaaaaa
     2041 cgtgaatatc acaccattgc cgggctgttg atggagtatt tgcagcgtat tccaaaacct
     2101 ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc
     2161 gtgcagaagg tgcagattat accgctgcgt aaggatggcg agatggagta cgaggtgtga
     2221 tggtatcggc cattttaccc aatgctgcgt aacgccttat aacttcagca atgaagcgtt
     2281 gtctgtggta aacggacatc acagcggggg tgtgggtccg gctgtaaatc gccgaatcgt
     2341 ttcctgtagg ctggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt
     2401 tacctcgcgc ccgtacccga tagccgaaag gaataagatg agggaaccgc gccagcggcg
     2461 atttaccgcc gggagcccgg gttgccaggg tggtggcgat tgagccaccc tggcacgttc
     2521 accggcttga gcgttccata gtagcgagga acatcaagtg aacggaacag ccttcgatct
     2581 tacatattca gaatataata gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt
     2641 atcaggccta caggacaggt cacctggtaa cctacatctt ctccagcaac ttcttcacat
     2701 ctttgccatt gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt
     2761 cgtcctgtag atgtttgcgc agtaactgat ccacttgcag gctgtaattg agttgctgcc
     2821 agttgccata aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt
     2881 ccccgttcca gccacccacg acccgaatat caaactgggt gtcgcaggtt tgatctgcca
     2941 gattcaacat gccttcccct gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta
     3001 acgtcacgac gccatccttc aacgtcaaat cggtggtaaa gcggtcaagc cgcgtcacgt
     3061 tatcgaagtt ttcagcggcc ttcacatcac cgccattacg ctctaccgct tgctgaatca
     3121 tctgctggaa gttcatccct tccatacgtg tgtcggtcat ctcaacatgc gcctgccctt
     3181 gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg
     3241 acatttttcc ggtcaacgaa atcggatagt taaacgcctt cagaatggta ccaatctcaa
     3301 cgttctccag ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc
     3361 ccggcagtga aacctttcca ccgttaagct tgccctgcag ttgagtaatt tccagcaaac
     3421 ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt
     3481 tactggcctg caacaaaata tcagctgtaa agccttgcag tccctgatag gccggttcat
     3541 caatacgcga agaaatgacc gggcgcggca acgttgattg gctctgcccc tgctgcgcgg
     3601 caccgttttc accattcgcg gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg
     3661 ggaattgcag tctcagctgc cattccggtt tctctgtcag cgtgacctgt gcttgcccgc
     3721 tcagcgtact gtcattggcg gtcaaactaa tttggttaaa tgaaaggcgt ttatgtgact
     3781 cctgccactg ggactggaaa ctcccctgtc cctgaatacc ttgttttggt aaatcggcac
     3841 cctgcaactg ccagttaatt tgttcaatag ccgctgttaa atcatgcgga taatcagaag
     3901 catctaccgt accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg
     3961 agaactcaaa tgagccacga tgttggggat cttgttccat ttgcaggcgg atattgcgga
     4021 ttgtcacctg ctcgtcatct tcatgctgga acaccagaac gctgtccgcc accttaagac
     4081 tggatatatc aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg
     4141 gtgcaaccgg agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg
     4201 ccccttttag catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca
     4261 cgtccagacg catgttgtcg gcgcgaacca gtggctggct tgcgccctgg gcggtgagag
     4321 acattcgccc ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga
     4381 gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat
     4441 tcggattcac cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca
     4501 tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga
     4561 ttcggctggc taccgcgccc tgctggttgc gatatttcgc atcttcacgg cggttgtaag
     4621 gtcgcgccgc cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac
     4681 gcagcgccag cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc
     4741 ccggatcgat acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac
     4801 gcccgtccag ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca
     4861 gcgccagctc tcctgggtga agatagaacg cctcgccctc gtcgagaacg atctcatcgc
     4921 tcatcacgcg gtcaagcgcg gcgctcactt catctttggg accgctcaga tcgataaacg
     4981 ctgccgtgtg accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc
     5041 cgttaatacg ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg
     5101 cttcaatatc tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta
     5161 acgccaatta cgtcaagggc ataccagctt atcactgaac ggtacacaat ttatcaagct
     5221 tattcaaaga actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttcccg
     5281 ccgcgcggca cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc
     5341 gggcgcacgg ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta
     5401 acgtcacgct tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag
     5461 aagttaagtt cgtcacgcaa acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg
     5521 accttcttcg gctcaacctt caccgtttct ttcatacgcg tatgttcaac atagctgtaa
     5581 accggcaggt caattgccga gccgcgtttc aacgcttgta aatgctcaag caacaggctg
     5641 tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg
     5701 ctttgatctt tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc
     5761 tcacgtaatt cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca
     5821 gcgataccga taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta
     5881 acctggatgt taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc
     5941 cgtctgatac cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acaaagaatt
     6001 cccggtggca aattacgttg atcagtttta tgtaaggtaa aaaatgttat acgcagttgc
     6061 gcaaattatc cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt
     6121 gtactagcat aaacacagaa acgaatactg gcgacctggc cttgcggata aagcggtaat
     6181 aagcaaacaa tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag
     6241 tttaggttta gtctcgttta tctttactct tgtctcgctt gagctttcgc agtttggcac
     6301 ccaactcgcc ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc
     6361 cgggcgcatg tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat
     6421 cctgcttttt tccaccagct cgctgaacat gacctggacg accatcaata ttgttgaagc
     6481 cgtggtcggg gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct
     6541 ggctgactgg ctgcgtctgg cattcggcag cgccattgtt ccgcctctgt tggggggtgc
     6601 ccctattaag t
//
LOCUS       DADLTI010000194         6437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLTI010000194 DADLTI010000000
VERSION     DADLTI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.196"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..552)
                     /locus_tag="JN755_003708"
     CDS             complement(<1..552)
                     /locus_tag="JN755_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7382346.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAV"
     gene            660..965
                     /locus_tag="JN755_003709"
     CDS             660..965
                     /locus_tag="JN755_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7382347.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            1164..2285
                     /locus_tag="JN755_003710"
     CDS             1164..2285
                     /locus_tag="JN755_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7382348.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYP
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            2319..2642
                     /locus_tag="JN755_003711"
     CDS             2319..2642
                     /locus_tag="JN755_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7382349.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(3234..4223)
                     /locus_tag="JN755_003712"
     CDS             complement(3234..4223)
                     /locus_tag="JN755_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382350.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(4298..4807)
                     /locus_tag="JN755_003713"
     CDS             complement(4298..4807)
                     /locus_tag="JN755_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382351.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(4909..5130)
                     /locus_tag="JN755_003714"
     CDS             complement(4909..5130)
                     /locus_tag="JN755_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382352.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(5377..6222)
                     /locus_tag="JN755_003715"
     CDS             complement(5377..6222)
                     /locus_tag="JN755_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382353.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT         1579 a         1628 c         1636 g         1594 t
ORIGIN      
        1 aaccgctgtg taatctaatt ttttgatcgg atccgttgat ggcgtggcgc tgacagataa
       61 tgttttgtcg ttacttttcg tggttttacc cagcggatac ccctgatcat catagcgata
      121 ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat cggtttccca
      181 actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac gtactctctt
      241 ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt accagagcga ccacggtatt
      301 attttccaga tccagtaatt cgagtgaatc aaaacagcct tcttccgaca aagtcccaga
      361 aacacgtttc gtcacttcac cttgctcatc cattaatgtc tgagtgaaat cttttaccgg
      421 accacgcagc ggatcaaaat cgaattcatt agagaaactg gccatctcag gggtaaatga
      481 aagtgttacc tctgtgcggt cacatcctgt gaggaatatc gcgagtaagc atggtagtaa
      541 tttgtatttc acaacagtca ccagagagta gagatgattc tcaatcatag tagcaaatac
      601 agtactttac acgttaaatg ctatgcttaa agaagttatc ttcgcgtaag gagcttatga
      661 tgaaactctc aacctgctgt gccgcgcttc tgctcgccct cgcctcaccc gcggtactcg
      721 ccgcgccggg atcctgtgag cgcatacaga gcgatatatc acagcgcatt atcaataatg
      781 gcgtacccga aagcagcttc acgttaagta ttgtacccaa tgaccaggtt gatcagcctg
      841 attcccaggt cgtcgggcat tgcgctaatg atacgcataa aattctctac aaccgcacca
      901 ccagcggtaa cgtctctgct cccgcgcagt ctacccagga tggcgcgcct gccgaaccgc
      961 agtaacacat tatcgactga acgccggata tgacacatcc ggcgatttga acatacaaca
     1021 taatctcacc ttattactca tacccttcta ttgatgtgga ttaataattc ttaacccaaa
     1081 atgggtagac tccctctatt gttagcgcgc taaatattca atatataaac atttatataa
     1141 cgataaagaa cagggagtga gttatgtcca aaaatgaccg aatggtgggc atcagccgca
     1201 gaacactcgt taaatcaacc gcgataggtt ctctggcgct ggctgcaggc ggtttttctt
     1261 taccgttcac cctgcgcagt gcagcggcag cggtacaaca ggccagcgaa aaagtgatct
     1321 ggggttcctg ttccgtcaac tgtggtagcc gctgtgcact tcgtctacat gttaaagata
     1381 atgaagtgac ctgggtggaa actgacaata ccggcagcga tgagtacggc aaccatcagg
     1441 tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat taatcatccc gatcgcttga
     1501 attacccaat gaaacgcgtg ggtaaacgcg gtgaaggcaa attcgaacgg attagctggg
     1561 atgaagccct ggatactatc gccagtagcc tgaaaaaaac cgtcgaacag tatggcaatg
     1621 aagctgtata tattcagtac ccttcgggga ttgttggcgg caatatgacc cgctcttcgc
     1681 catcagcctc ggcggtcaaa cgcctgatga actgctacgg cggttcactc aatcagtatg
     1741 gctcctacag caccgcgcaa atttcctgtg ccatgcccta cacctacggc agtaatgatg
     1801 gcaacagcac cacggatatt gaaaacagca agctggtcgt gatgtttggt aacaacccgg
     1861 cagaaacccg catgagcggt ggtggcatca cttatcttct tgaaaaagcg cgcgagaaat
     1921 cgaacgccaa aatgattgtt atcgatccgc gatataccga tacggctgcc ggtcgtgaag
     1981 acgaatggct ccctattcgc ccgggcaccg atgccgcgct ggttgcgggt attgcctggg
     2041 tattgattaa cgaaaatctc gttgatcaac cttttctcga taaatactgc gtcggctatg
     2101 acgaaaaaac cttacccgca gatgcaccca aaaatggtca ctataaagcc tatattcttg
     2161 gtgaaggtga cgataaaaca gcaaaaacgc cgcagtgggc ttcgcaaatt accggtatcc
     2221 cggtagaccg tatcatcaaa ctggcgcgtg aaattggcac aacaaaaccc gcttatatct
     2281 gctagggctg ggggccgcaa cgccaggcaa acggcgagct gactgcacgc gctattgcta
     2341 tgttacctat tttgacgggc aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat
     2401 cgacctatac cattacgata gaacgcctac cggtgctgga taatccggtc aaaacgtcaa
     2461 tctcctgctt cagctggacg gatgctatcg atcatggccc gcaaatgacg gcactccgcg
     2521 acggtgtccg gggcaaagat aaactggatg tgcccattaa gttcatctgg aactacaagg
     2581 acttgaagcg gttactcgtt agagtcacga gagaccttag taggcctgag gtcctgcatt
     2641 gagtcaagac cttgtgtcat tctttgtgcc agacttgtgt cattagacgg gatgtctaga
     2701 cggctggtca ggcccccggt ggcctctttg tcaggactca gggtggcacc ggggaacatt
     2761 ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt ccaaaactca tcgttagctg
     2821 ctcgccagct cacagccgaa cccgaactgt aagcacgacc accagcacca gcttcagcag
     2881 gaagacgaca agatagactt tatgcaggtc catgcgtcct cctgtgtcta ttgtaccact
     2941 gcactagata actagtacaa aataaagtcc agaccgaagg cccagaccat agttaattaa
     3001 tcacattgat aatccaccac ctgaagtcct gccaacattc agtccagcct tacccatgct
     3061 aggagtctta accggggtgt cgttctgtgt ctccaccact ggtgccgcat caggctctac
     3121 aggaagctcc acagcaggcg cagggaccgg aggtgatacg gatgtaccac caccagcgga
     3181 gaagccctta gcgatggacc ggaggccttt actaatgctc ttacccattg ctgttacgct
     3241 tcggtcttgg tgaacttcag gcgtttgact acaggcagga ctgcggttgc agcgccagtg
     3301 tccagcttaa ctgtcgtagc agcttcagcg acaacgaaga ccttaccgag gatttccaag
     3361 tcgtgggact gagagtcgcc atcacgtgaa aggacgaccg taatattctc accgtcgtga
     3421 cggaagcagc cgaagctcag ggccgtgaag cgtttagcca gcatgaacac acctttgtcc
     3481 tcaccgttgt aatacgcgat gtcggtaccc agcatatcca cgatagcgtc ttcaccagag
     3541 aagccctgtt tagcagccag acgggacagc gctttcagtt gggactcatg ggcgatgatg
     3601 ccgtaatcag acacttcaga gccgaaagag gactcggtgt tctcaaacag agcgtccatg
     3661 atagcgtctg ccttagtggt caggcttcca gtcaggactg gcagctctac ggtcttcagc
     3721 agcttggagg actccagaac gtcaaccatg cgagcgttga tggtacggac cacattctca
     3781 cagatgttca tagcgtcctg ctggagtgtg ccatgagtga aaccagtgga ggacttggcg
     3841 tcccacagtt tagtttgctc cgagatgagg attgcctgtt cgataggttc gacgacaatc
     3901 tcctgaccag acggaacggt cacgacttca ggtcgctcac caccgcgtac agggacgacc
     3961 acagagcggt cagacagggt gtgggcgatg attggggtag gcaggctatt gcgggtaggc
     4021 aggacttcta cagggaacgg aatgttatcc tgagcgtctt taagtacacc gttcagcata
     4081 gccttaaccg tcttagctaa ctggagggca tagacctgtg ggtcagcgtt agttggcaga
     4141 ccttcgttaa cctgaatgtt gtcggtgtcg gtgatacgta ctggctcaat gccgaactga
     4201 tagctatctt taatcatggt cataataatc aatctcctaa attagtttta gatactgtgg
     4261 gtcccgacat caggtcagga ccacttgtgt gtctgtctta gaggctgtcc atccagttac
     4321 cgcctgactc aatgtcagcg acaatagcgg cacgggagcg accagcggaa tgggtagcac
     4381 tgcggaggtt ctcaccatag gtccggcgct cagctttgac ctgttcgcgg cgctgtagct
     4441 ctttctgcca ctctgcatcg gtcatgttct cttcacgcat acgctcagcc tgaatgcgct
     4501 gctctttagc gatacgctca gcctcggctc gcttgttctg ctggcgacgt tggtaggcgg
     4561 tcatcaggtc attgctgaag aagtttccaa tggtcacgcc agcttcagcc agagcttcca
     4621 gtaattccat ggactcgcgg ctggtggcag gcagtcctga caggtagccc ggaatcaggg
     4681 tcttaatagc gaaggacagt gggtaggact gaccgtccag actcacagtg ttttcgtaga
     4741 gattgatgtc ggtgacatgg agtttccagt cattgttgtt gaataacgca ctagctttgc
     4801 tagacatagt aattactcct cttgagagtt gagactatcg gtaccgcaga cacagttatg
     4861 cctacgggtc tccccgcatc ggtcatctca aaaggtttaa attaggggtt agtgagtggc
     4921 gtccagctta gcgaacgctc ggcgtaactg attgttcagg tcacggtcga ccagcaggtc
     4981 tatcagttcg tctcgactat agaaaggacg ctcagggtcc tctacgccca tccccagcat
     5041 tgacaggagg cgacgggtag gcagaccacg gtaacggaga gtagcctttc cgatgggtgt
     5101 ccgtgggtcg atatgttgtt tagttgtcat tgttagaagt cctcgttaga ccaatcatcc
     5161 acgtcaaatt cactggtgat aggagcttgg aagttaggta gaccctctgg tatctctaga
     5221 ggcagtcgct cgtcgactct cagtgccatc tcttcgtgta tatgccgaag cagagaagtc
     5281 agtaagatag ggtccgcagg atgctcaggg ccgtgtctct caatgaaaca tatggttgtc
     5341 gctattgact ccataatcag ttcatcagtg agtcgcttag aacccatagt cagacccctt
     5401 gcccgctgct gctttctggg actttgtagc cttaccattc aggtgagaat ccaccagacg
     5461 acggacaacc ttgatattgg atgtgcgcat gtcatgagcg atatccttcc agttatcctt
     5521 cagccagtca taacccttgc gtagaatctt gatggtaggc tctttgtcgt taggctgtgg
     5581 ctgtagacga tagtagccgt tggtctcacc tttcttattg aagatggctc gtggacgtcc
     5641 ggtcgatagg tctatatccg tagtgccaaa ctcagtaaca cgcttggtgt agaatgggtt
     5701 aggacgacac agggtcttca gatatccagt caggacgcta gctgtcagca tcttagagct
     5761 ggtcaactta agatccagag ctttaataac ctcggtaggc gtggcgcctg acttggagtc
     5821 cagcttggag tcgaggacga tagcaccggg cgcatagcag atagctttgg tctgtgcgac
     5881 ctgaagttgc tgggcctgaa gttcgaggac ctcttgctcc ttctggcgaa tataggctag
     5941 gaccttacga cgcacagctt tagactcacg catcccgacc agcagaagct ggtcagtgtt
     6001 gagggtatag cttttaacgt cctgcccgaa gcttacgaaa atttcgtagg ctacattttg
     6061 accgtccagc tcatcttcaa tacgggacac aaagtcgttg ttacggacag gcttctcccc
     6121 agcctccaca cgagccgggt tgatgacctt agtcaagaaa tcgtagctac tcatagacac
     6181 aagctggtca aggttgatga cgctaacttc gttggtattc ataaaggatg ctcctttggt
     6241 aaagcacttt ctgtctccat gtggagtccg cacctattca atgcatccaa acggacccac
     6301 caaaaagtgc ttaacgaaag tgaatagggt taatgttgat tagtgttggt ctggagtgac
     6361 catgttctcc atgtgcatat aaggcgagac cgcatgtcag gacgagctgt aaggacccca
     6421 gatggtgagc ggtgata
//
LOCUS       DADLTI010000195         6325 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLTI010000195 DADLTI010000000
VERSION     DADLTI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.197"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(274..1305)
                     /gene="rsmC"
                     /locus_tag="JN755_003716"
     CDS             complement(274..1305)
                     /gene="rsmC"
                     /locus_tag="JN755_003716"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7382354.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            1408..1821
                     /gene="holD"
                     /locus_tag="JN755_003717"
     CDS             1408..1821
                     /gene="holD"
                     /locus_tag="JN755_003717"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7382355.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1790..2236
                     /gene="rimI"
                     /locus_tag="JN755_003718"
     CDS             1790..2236
                     /gene="rimI"
                     /locus_tag="JN755_003718"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7382356.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2251..2928
                     /gene="yjjG"
                     /locus_tag="JN755_003719"
     CDS             2251..2928
                     /gene="yjjG"
                     /locus_tag="JN755_003719"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7382357.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3019..4608
                     /gene="prfC"
                     /locus_tag="JN755_003720"
     CDS             3019..4608
                     /gene="prfC"
                     /locus_tag="JN755_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7382358.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5001..5606
                     /gene="osmY"
                     /locus_tag="JN755_003721"
     CDS             5001..5606
                     /gene="osmY"
                     /locus_tag="JN755_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7382359.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5733..5894
                     /locus_tag="JN755_003722"
     CDS             5733..5894
                     /locus_tag="JN755_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7382360.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6016..>6325
                     /locus_tag="JN755_003723"
     CDS             6016..>6325
                     /locus_tag="JN755_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7382361.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     L"
BASE COUNT         1549 a         1617 c         1680 g         1479 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt atgcggcgac agatatcaga tactcaacct ttcttcgcct ggcgggtcat
      301 aatggcgcga tataccttga agcgcccggt ttgcgtgata acttcatgga agccaaatgt
      361 ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc
      421 gctattaaga tgacgcaccg cgccacgaat cagcgtttgc gccgcatcca ggctggtttg
      481 catcccatcg tggaacggcg ggttggagat gatcatatca aaacaacctt tcacctcgga
      541 aaagacgttg ctggcaaaga cttcaccttc aacaccgttg gctgcaagtg ttgcgcggct
      601 ggcttctacc gccggggcag agacatcgca caaggtgaga cgaattttcg gcgaatggcg
      661 cgcaaaggca actgaaagta cacccgcgcc acagccgaca tccagcactt tacctttcgt
      721 gtgcggagtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt cgcggctaaa
      781 cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc
      841 cgcatcaaat actggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct
      901 gtcgacttta ttcaacggcg cataatctgc cagcatctgc tcagcgctgc gcacgccgct
      961 gcggttctcg ccaacgacaa aaatatctgt ccccactggc agcagagaaa gtaaattcat
     1021 caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc
     1081 tgcgacatca ttcgccgtgg cgaccagact aaaacgggcg ttatccccca tctggcagct
     1141 taacacctgc caatggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg
     1201 cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc
     1261 actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat
     1321 tgatactggc ggtgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg
     1381 cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa
     1441 ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt
     1501 gcgagcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgactgat
     1561 cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt gctgcaactg
     1621 acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt
     1681 actgacgaac cgctatcatt ggaaggcgct caggtggcat caccggcgct caccgaatta
     1741 cgggcaaacc caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat
     1801 ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc
     1861 ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca
     1921 gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc
     1981 tacattgttc aatattgcgg tcgatcccga ctatcagcgt caggggctgg gtagggcgct
     2041 gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacactat ggctggaagt
     2101 ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac
     2161 gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt
     2221 gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc
     2281 gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac
     2341 agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg
     2401 gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg tttcgagagc
     2461 tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat taatgcgatg
     2521 gcggaaatct gtacgccgtt gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac
     2581 gctaaaatcg gcatcatcac caacggtttt agcgccttgc agcaagtgcg tctggaacgg
     2641 acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa
     2701 ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc
     2761 gtgctgatgg ttggcgacac tgccgagtcc gatattctcg gtggcatcaa cgccgggctt
     2821 gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcccacctgg
     2881 accgtttctt cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc
     2941 cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt
     3001 actcaagaag aaagaattat gacgttgtct ccttatttgc aagaggtggc gaagcgccgc
     3061 acttttgcca ttatttctca cccggacgcc ggtaaaacta ccatcaccga gaaggtgctg
     3121 ctgttcggac aggccattca gaccgccggt acagtaaaag gccgtggttc caaccagcac
     3181 gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg
     3241 atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac
     3301 ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac
     3361 gccgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac
     3421 acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg
     3481 ctcgatgaag ttgagaacga gctgaaaatt ggctgcgcac cgatcacctg gccaattggc
     3541 tgcggcaagc tgtttaaagg cgtttaccac ctttataaag atgaaaccta tctctatcag
     3601 agcggtaaag ggcacaccat tcaggaagtc cgcattgtta aagggctgaa taacccggat
     3661 ctcgatgctg cggttggtga agatctggca cagcagctgc gtgacgaact ggaactggtt
     3721 aaaggcgcgt ctaacgagtt cgacaaagag ctgttccttg cgggcgaaat cactccggta
     3781 ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctgaatgg cctggtggag
     3841 tgggcgcctg cgccgatgcc gcgtcagacc gatacccgta ccgtagaagc gagcgaagat
     3901 aaatttaccg gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc
     3961 gtggcgttta tgcgtgtggt gtccggtaaa tatgaaaaag gcatgaaact gcgccaggtg
     4021 cgcactgcga aagatgtggt gatctccgac gcgctgacct ttatggcggg tgaccgttcg
     4081 cacgttgaag aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg caccattcag
     4141 atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca
     4201 ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgctcaaa
     4261 gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccaat ctccaacaac
     4321 gatctgatcg ttggtgcggt tggtgtgctg cagtttgatg tggtggtagc gcgcctgaag
     4381 agcgaataca acgttgaagc agtgtatgag tcagtcaacg ttgccactgc ccgctgggta
     4441 gaatgtgcag acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg
     4501 cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggca
     4561 caggaacgtt atccggacgt tcagttccac cagacccgcg agcattaatt cttgcctcca
     4621 gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt
     4681 tcttaaaaca ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt
     4741 gatctatatt taacaaagtg atgacatttc tgatggcgtt aaataccgtt caatgcgtag
     4801 atatcagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagagac
     4861 tgtaatggtc gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga
     4921 gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gctaggctca aattacgagc
     4981 aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg
     5041 ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcgcaga
     5101 ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca
     5161 tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca
     5221 agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg
     5281 aaagtcaggc ccaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggcgtgacct
     5341 ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag gactacgcgg
     5401 gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt
     5461 cacgtaaagt gaaagttgaa accaccgatg gcgtggttca gctctccggt accgtagatt
     5521 ctcaggcaca aagtgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg
     5581 ttaaaaatga tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg
     5641 aggcgcgatg tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag
     5701 cggtgacatt aactatggta aaggagatgc ttatgtttcg ttggggcatc atatttctgg
     5761 ttatcgcgtt aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg
     5821 cagctaaaat tgtctttgtc gtcgggatta ttctgttcct agtgagtttg ttcatgggcc
     5881 gaaaacgacc ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac
     5941 tggcttttgc ggttttagcg aataataaat tgcgttactt tgtctttctt tcacaacaaa
     6001 aacaggaagg cagaagtggg gcagagaata cctgtaacgc ttggtaatat tgcgccgttg
     6061 tcgctaaggc cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt
     6121 ggaattttca cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat
     6181 ctttatctcg gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc
     6241 ggttacgcgc gcaaagtcat catgcgctat accacaaaac gcgaattttt tgatccattg
     6301 cgctttgtcc gtggaggaaa tctta
//
LOCUS       DADLTI010000196         6321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLTI010000196 DADLTI010000000
VERSION     DADLTI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.198"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..170
                     /locus_tag="JN755_003724"
     CDS             <1..170
                     /locus_tag="JN755_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7382362.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            275..1627
                     /locus_tag="JN755_003725"
     CDS             275..1627
                     /locus_tag="JN755_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7382363.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1640..2398
                     /gene="chbG"
                     /locus_tag="JN755_003726"
     CDS             1640..2398
                     /gene="chbG"
                     /locus_tag="JN755_003726"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7382364.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JN755_003727"
     CDS             complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JN755_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY7382365.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4889..5152
                     /gene="cedA"
                     /locus_tag="JN755_003728"
     CDS             4889..5152
                     /gene="cedA"
                     /locus_tag="JN755_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7382366.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5429..6245
                     /locus_tag="JN755_003729"
                     /pseudo
     CDS             5429..6245
                     /locus_tag="JN755_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1617 a         1348 c         1600 g         1756 t
ORIGIN      
        1 tccgtattaa ttttgccaaa aaacaactgg aaatgaccaa ctattccgtg acggatattg
       61 cgtttgaggc cggttatagt agcccgagtt tgtttattaa aacgtttaag aaattaacgt
      121 cctttacgcc taagagatat cgtaagaaat tgactgaatt taatcagtaa gtcgttatac
      181 ctgacaattc acatatctgt cctgttgctg gattatttat gtccgggggg gcagatatgc
      241 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
      301 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
      361 cgaattgccg gtcagcgaat tatggctggt ggatgtcgag ggtggcaaag ctaaactgga
      421 tattattttc gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
      481 taaaacgctg gattgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
      541 cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc atggttatct
      601 tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca ttccggtgat
      661 ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaacttcac
      721 taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta aacgctttat
      781 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc tgatgctgaa
      841 agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt tcattaagaa
      901 tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg catccgggca
      961 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     1021 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     1081 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt
     1141 cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact
     1201 ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca acgctatcta
     1261 caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat
     1321 tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg cgacgccaca
     1381 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca ttaaaggctt
     1441 cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt tactggcgct
     1501 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     1561 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     1621 acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg attttggctt
     1681 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac
     1741 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc gtgatgaacc
     1801 gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc
     1861 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     1921 tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt tcattgagct
     1981 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     2041 tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     2101 gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     2161 cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg atgacgcaag
     2221 ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     2281 ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga catcagcgtc
     2341 gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg atgtgtaagt
     2401 aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc cggaattttg
     2461 tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa cagttcatcc
     2521 ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg gtcagctacc
     2581 ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt aaggtgtttg
     2641 taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc gatattgccg
     2701 caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt agcggctata
     2761 ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag cagtttggca
     2821 tgaacgcctt tggccttcag ggccttgaga atggcaagaa ggtctgccga tctcacttca
     2881 tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaaa
     2941 cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat attcagctgg
     3001 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     3061 tgcgccagct ggtcaacaac gtgctcacga atatacggac gaacgacttt gcttaactca
     3121 aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag ccagaacaga
     3181 cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt attgccttcc
     3241 acgcgctcct ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt ttcgcgcggc
     3301 cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat ccccatacga
     3361 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatctca
     3421 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa caaacgtccc
     3481 tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg atggaatgct
     3541 gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat tttgccgaca
     3601 cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag atcgaagtcg
     3661 aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc cggaaaatcg
     3721 cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg tccggtgagt
     3781 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     3841 acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc gaagccttcc
     3901 atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac gttgtgcaga
     3961 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     4021 attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     4081 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     4141 aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc gccaccctga
     4201 acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag gaaatccgct
     4261 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     4321 gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt ctcgcgcaga
     4381 ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt ttgatcatcg
     4441 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     4501 tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc aggggcggta
     4561 ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc ctcaggtgcc
     4621 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     4681 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta ctgaaagggc
     4741 cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagattcc gcttcggaga
     4801 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     4861 ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     4921 gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     4981 agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg gcaaatatgt
     5041 tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     5101 tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt aaggattaca
     5161 tacctcctgg ttttgccggg agttacagga cctgaaattc tgcatttcgt tgctacattc
     5221 attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa ataagtgatc
     5281 ttttttcttt aattgtttgt ttattttaaa atggttaagt tttgctaatc atgatgagtt
     5341 atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat ggcttagatt
     5401 taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta caaacttaat
     5461 acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt ttaaagaaag
     5521 gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat gtgactgcga
     5581 tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca ttaactatga
     5641 tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg acaatgttaa
     5701 atatagagtg ataattaata atctttatgg cagtgagttt cggataagag atattaatga
     5761 ggattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga cgaatttgat
     5821 caatataaaa aataggttgg ttattcatta tttaaggact atatactatg aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtttcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
     6181 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
     6241 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
     6301 gtttgcttca gccactggct g
//
LOCUS       DADLTI010000197         6302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLTI010000197 DADLTI010000000
VERSION     DADLTI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.199"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JN755_003730"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JN755_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7382367.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="JN755_003731"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="JN755_003731"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7382368.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="JN755_003732"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="JN755_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7382369.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /locus_tag="JN755_003733"
     CDS             3240..4580
                     /locus_tag="JN755_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7382370.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..>6302
                     /locus_tag="JN755_003734"
     CDS             4658..>6302
                     /locus_tag="JN755_003734"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7382371.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
BASE COUNT         1628 a         1605 c         1549 g         1520 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtaacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg gtcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gt
//
LOCUS       DADLTI010000198         6241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLTI010000198 DADLTI010000000
VERSION     DADLTI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.200"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            7..312
                     /locus_tag="JN755_003735"
     CDS             7..312
                     /locus_tag="JN755_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382372.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(388..852)
                     /locus_tag="JN755_003736"
     CDS             complement(388..852)
                     /locus_tag="JN755_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7382373.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /locus_tag="JN755_003737"
     CDS             complement(849..1724)
                     /locus_tag="JN755_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7382374.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JN755_003738"
     CDS             complement(1721..2410)
                     /locus_tag="JN755_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7382375.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JN755_003739"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JN755_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7382376.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JN755_003740"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JN755_003740"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7382377.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JN755_003741"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JN755_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7382378.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1581 a         1692 c         1548 g         1420 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt acctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DADLTI010000199         6224 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLTI010000199 DADLTI010000000
VERSION     DADLTI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.201"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(283..987)
                     /locus_tag="JN755_003742"
     CDS             complement(283..987)
                     /locus_tag="JN755_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7382379.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JN755_003743"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JN755_003743"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7382380.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JN755_003744"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JN755_003744"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7382381.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JN755_003745"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JN755_003745"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7382382.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JN755_003746"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JN755_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7382383.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JN755_003747"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JN755_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7382384.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JN755_003748"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JN755_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY7382385.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..>6224)
                     /locus_tag="JN755_003749"
     CDS             complement(5324..>6224)
                     /locus_tag="JN755_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382386.1"
                     /translation="RPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGL
                     LDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYL
                     HSDIDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERS
                     LIQTIGRAARNVNGKAILYGDKITPSMAKAIGETERRREKQQKYNEEHGITPQGLNKK
                     VVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELEGLMMQHAQNL
                     EFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1492 a         1648 c         1556 g         1528 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgttttta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttcggtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtaat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acgg
//
LOCUS       DADLTI010000200         6171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLTI010000200 DADLTI010000000
VERSION     DADLTI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.202"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            171..818
                     /gene="fucA"
                     /locus_tag="JN755_003750"
     CDS             171..818
                     /gene="fucA"
                     /locus_tag="JN755_003750"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7382387.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            846..1994
                     /gene="fucO"
                     /locus_tag="JN755_003751"
     CDS             846..1994
                     /gene="fucO"
                     /locus_tag="JN755_003751"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7382388.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JN755_003752"
     CDS             complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JN755_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7382389.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JN755_003753"
     CDS             complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JN755_003753"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7382390.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4338..5627)
                     /locus_tag="JN755_003754"
     CDS             complement(4338..5627)
                     /locus_tag="JN755_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7382391.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1570 a         1507 c         1644 g         1450 t
ORIGIN      
        1 agagaacttg cagttattta ctgtgattag ttgagctagg tcacaaaata taagcttgtg
       61 aatttctttc ttgttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt
      121 gtgaaaggaa caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa
      181 ataaacttgc tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc
      241 aggggacagc ggggaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag
      301 gcattccata tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggtaaac
      361 atgaggaagg aaagctcccc tcaagcgaat ggcgtttcca tatggcagcc tatcaaagca
      421 gaccggatgc caacgcggtt gttcacaatc atgccgttca ttgcacggca gtttccattc
      481 ttaaccgatc gatccccgct attcactaca tgattgcggc ggctggcggt aattctattc
      541 cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc
      601 tcaaaaatcg taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggtgaatc
      661 tggaaaaagc gttatggctg gcgcatgaag ttgaagtgct ggcgcaactt tacctgacga
      721 ccctggcgat tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg
      781 agaaattcaa aacctatggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa
      841 ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg ggtgctgttg
      901 gggctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata
      961 aaacgctggt gcaatgcggc gtggtggcga aagtgaccga taagatggat gctgcagggc
     1021 tggcatgggc gatttacgac ggcgtagtgc ccaacccaac aattactgtc gtcaaagaag
     1081 ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt ggtggttctc
     1141 cacaggatac ttgtaaagca attggcatta tcagcaacaa cccggagttt gccgatgtgc
     1201 gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca
     1261 ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac
     1321 ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgatgctg
     1381 acatgatgga tggtatgcct ccggcgctga aagccgcgac gggtgtcgat gcgctcactc
     1441 atgctattga ggggtatatt acccgtggcg cgtgggcact aaccgatgca ctgcacatta
     1501 aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg
     1561 gagaagaaat ggcgctcggg cagtatgttg cgggtatggg cttctcgaat gttgggttag
     1621 ggttggtgca tggtatggcg catccactgg gcgcgtttta taacactcca cacggtgttg
     1681 cgaacgccat cctgttaccg catgtcatgc gctataacgc tgactttacc ggtgagaagt
     1741 accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg gaaggggcgc
     1801 gtaatgccgc tgttgaagcg gtgtttgccc tcaaccgtga tgtcggtatt ccgccacatt
     1861 tgcgtgatgt tggtgtacgc aaggaagaca tttcggcact ggctcaggcg gcactggatg
     1921 atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc
     1981 ataccgcctg gtaaatgcgc tgatgtgata atgccggata cgatttgcgg cgtatccggc
     2041 tcgccgttac cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg
     2101 taagcgggca atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg
     2161 ccacttttcg gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac
     2221 cagcagctgc gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct
     2281 gatccccgca agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc
     2341 gataaatggc gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga
     2401 aagtaactga cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggccagtgt
     2461 cactttgacc gccagtgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca
     2521 cgggacgccg cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc
     2581 cggcattggc ggtcgatccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact
     2641 gcggttttca tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg
     2701 atcgagcgca tgctggcagg tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg
     2761 gataagattc agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat
     2821 caggcgataa gggtaacatt cgatgccgga acaggctatg gctggttgag aaaacaggaa
     2881 gaggcttcgc aaaacgaggc ctctgggaga gattaatcgc aggcaacgat cttcatcgcc
     2941 aggccgccgc gagaggtttc gcggtacttg gcgttcatgt ctttacctgt ttcgtacatg
     3001 gtttcgataa ctttatcgag gcagacgcgc ggttcgctgg tacggcgcag tgccatacgc
     3061 gcggcgttca ccgctttcac tgctgcaatg gcgttacgct caatgcacgg tacctgaacc
     3121 tgccccgcga ccgggtcaca agtcagaccg aggttgtgct ccatcgcgat ttccgctgcg
     3181 atgcaaacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc cgccatagag
     3241 cacgccacgc caacttcacc ctggcaaccc acttcggcac cagaaatcga cgcgttcatc
     3301 ttataaagag aaccaatggc gctggctacc agcaggtaac gagccagtga gttagcgttc
     3361 acttcgcgga taaacttatc gtagtacgcc agcacagccg ggataatccc gcacgcaccg
     3421 ttagtcggcg cagtcaccac gcgaccgcca gcagcgttct cttcgttcac tgccagtgca
     3481 aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc ctggctgacc
     3541 agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg caacacgcct
     3601 tcggtggaaa taccgcgctc aataccgcca cgcatgactt cccagacgtt cgccaggtgc
     3661 tgttccagct cttctttact gtgcagcgcc agctcgtttt tcatcatcag gccagagagt
     3721 gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta cggataagga
     3781 acttcaaccg gtgcgctatc ctgctggcca aaatgctctt catcaacgat aaagccaccg
     3841 ccaatagagt agtaagtctg gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc
     3901 ataccgtttt catgcagaga aaggttgtct gcatggaagt tcatgcactg atcaaccggg
     3961 aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt cacatcctga
     4021 ataaaaccgg ggatggaatc gatatccacg gtatccggca ggttacccgc caggcccata
     4081 ataatggaga tatctgtgtg gtggccttta ccagtcagag agagcgagcc gtacacgtca
     4141 accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt
     4201 ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc gattttgaaa
     4261 atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag cgccgcccga
     4321 aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat tgcaatcagc
     4381 cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat tgccggtact
     4441 ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg gccgcccagg
     4501 gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt cgttaccagc
     4561 atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc tttaccacgc
     4621 agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag
     4681 gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgataac cggtgcgtta
     4741 aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc agccaggtct
     4801 gccggagtca ggctcagtac gcagctgaag acgaagaaca ttacggtcag caccatcatg
     4861 atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc gtactcttca
     4921 cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa caccattacc
     4981 ggaattgcca gccacagggt catccacaga ccgtttccgg ttgcagatgc agtgtccaga
     5041 gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc cagcagcatc
     5101 agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat ctgctcacca
     5161 aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc acgcggcggt
     5221 ggcgtcatac ccagctggtg agacatgaag ctttcaacgg tattggtgat tgccacgcta
     5281 taaaccagca ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg
     5341 ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac
     5401 agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc
     5461 atgatgatca gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc
     5521 gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca
     5581 ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg
     5641 aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa
     5701 atgaaatctg tctatcggcg atggcttaat catttaccgc tgtaataatt tgagggcgga
     5761 aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt
     5821 ggaagataga ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaaggg
     5881 caatatttgc ccattaaacg acatctgact tcttccgggg cgcgcaatat atgtaggttg
     5941 aactgaaaaa atcaatactt ttagttaatc atggcgataa tttctttttc atagcgataa
     6001 aatacggttt atgaaaatag ggaaacgttt gcgttgcaac ataaattgta tggcatatta
     6061 actgtgatga tatttgtcgc gtgagggtgg taagaaacat tcatataaca aatgaaatga
     6121 gttgatgagt aagtgaccaa aaacgagaaa acccggcaac aaacgtcacc g
//
LOCUS       DADLTI010000201         6135 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLTI010000201 DADLTI010000000
VERSION     DADLTI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.203"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..367)
                     /locus_tag="JN755_003755"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="JN755_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(707..1261)
                     /locus_tag="JN755_003756"
                     /pseudo
     CDS             complement(707..1261)
                     /locus_tag="JN755_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1404..1526
                     /locus_tag="JN755_003757"
                     /pseudo
     CDS             1404..1526
                     /locus_tag="JN755_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1788..2354)
                     /locus_tag="JN755_003758"
     CDS             complement(1788..2354)
                     /locus_tag="JN755_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382392.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2636..2950)
                     /locus_tag="JN755_003759"
     CDS             complement(2636..2950)
                     /locus_tag="JN755_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382393.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3028..3138)
                     /locus_tag="JN755_003760"
                     /pseudo
     CDS             complement(3028..3138)
                     /locus_tag="JN755_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="JN755_003761"
     CDS             complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="JN755_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7382394.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4510..4647)
                     /locus_tag="JN755_003762"
                     /pseudo
     CDS             complement(4510..4647)
                     /locus_tag="JN755_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4729..5027)
                     /locus_tag="JN755_003763"
                     /pseudo
     CDS             complement(4729..5027)
                     /locus_tag="JN755_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5616..5775)
                     /locus_tag="JN755_003764"
                     /pseudo
     CDS             complement(5616..5775)
                     /locus_tag="JN755_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5779..6135)
                     /locus_tag="JN755_003765"
                     /pseudo
     CDS             complement(5779..6135)
                     /locus_tag="JN755_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1651 a         1353 c         1247 g         1884 t
ORIGIN      
        1 tgacagtgtt gttgcgcctt gctgccaacg aagctccact gcccatcaag ttcacagata
       61 agggcaacat cagcgtgagc gactggcgca gatgttatcc gccggggcgg gagcttttta
      121 atgtccgtat gacggtattg atgccgattt tcagcgtacg ggcagtatcg cgaactccgg
      181 caccgttgaa agccatttca gtgatttggt ttttaactcc cggtttacgg gcctcgtaac
      241 agtaagtgag ctgaaacacc cggtgacagt cacggcaacg gaatcgatca tgtccttttg
      301 ggttctgccc gtgacggtaa acctgaacgg actggcaacg ggggcaatga aagttaactg
      361 tgaccatgaa agaaccttaa aagcgggtat tataacccct aattcaacta attggaggca
      421 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      481 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      541 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      601 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      661 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      721 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      781 aatcacattc gatataatta aacactgttg cccgcattat ttcccggctg ataaagtgtt
      841 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      901 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      961 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
     1021 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1081 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1141 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1201 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1261 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1321 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1381 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1441 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1501 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1561 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1621 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1681 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1741 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1801 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1861 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1921 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1981 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     2041 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2101 tctgcacaaa acttatcaat acattggttc accacccggg atacgtagac ttttttttca
     2161 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2221 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2281 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2341 gatgttccaa gcattataat cactttgtca tgtaaaatag taacattgtc attattttag
     2401 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2461 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2521 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2581 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2641 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2701 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2761 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2821 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2881 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2941 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     3001 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3061 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3121 aaacactgta tatttcattt gtggagtctc cagtctgagg gggctttatg cattccgtat
     3181 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3241 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3301 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3361 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3421 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3481 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3541 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3601 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3661 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3721 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3781 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3841 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3901 tactccttat tattctttgt cggaatggat atgctctgta ttttggccaa ttttctgaaa
     3961 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     4021 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4081 attattaggt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4141 tttgttcatc ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta
     4201 gtctattgct aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt
     4261 ctttcgattt cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg
     4321 agtccgttta ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc
     4381 aaacttgata ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt
     4441 ctgtgatgct gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc
     4501 ctaataccgc tacactttgt ccagactatg tttttctccg ggaagtgggc taaccgtctc
     4561 cataaagtga cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt
     4621 tattgtctca tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcacc
     4681 agacagcctg aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt
     4741 cttcagctgt tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg
     4801 ttcatctcca gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct
     4861 aagctgattt ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt
     4921 ttcagcatcc aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca
     4981 ttagctaaag cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca
     5041 tttgatatca aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc
     5101 tatgatcttc gaatcctgaa gattatagtg aaaataagtt ttcacgttga aatcccacat
     5161 agttagtata tgataaataa tcatggatga cctgattaac ctcttggggt agtgctttta
     5221 tagaagaata aatattctac taatttaata catcacctaa aatagtattg ttttttatgg
     5281 ttggttgaag gtcgtggtga tgatgttagt ccttttgaga atattccagt gccagatctt
     5341 acttataata aaggggcttg gcagtttgga taaactattg ttcttattcc actaatacaa
     5401 actttttgta aagtatatta aatatacttc atttcatact gaaatccccg gaagtattca
     5461 atcaaacaat tacaccataa aaggaagagg gggccctaat taagttgtca agcatgttta
     5521 tgatacctgc agggtataaa ttttctggct gcaagcagac acatataaaa cgtctttatt
     5581 gatcctaccc acataatatg gacacagtcg taagtgaggc tctggttttc aaattgttcc
     5641 ggactgagac caccacacca acggtgccgt cgccagcgat tgtaatcgca ctcgatataa
     5701 ctaaacactg ttgcccgcac tatttcccga tggataatag tgttctccgt ggagacattc
     5761 cattttcagc gaatgaattt tcatctgttg ctgtatccag tcatacagtg actgcatcag
     5821 tggcgcggct ctggcttttc ttgccgccag acgctgttct gctgaacatc cccggacttc
     5881 tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg
     5941 cactcttgca tgcacatcgt ggatttttct ccggacatga gccatacatg cggcttccgt
     6001 tattctgccg gattcgtaca acgcccggta accaccgtaa gcatcggcct gaagcactcc
     6061 gctgtaaccg gccagatgat tctgtggatg gatacctttc cggtccggtg agtacgcgaa
     6121 ccagaccgcc ggagg
//
LOCUS       DADLTI010000202         6096 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLTI010000202 DADLTI010000000
VERSION     DADLTI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.204"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..771)
                     /gene="osmF"
                     /locus_tag="JN755_003766"
     CDS             complement(<1..771)
                     /gene="osmF"
                     /locus_tag="JN755_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7382395.1"
                     /translation="MPLLKLWAGSLVMLAAVSLLLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEY"
     gene            complement(1023..3320)
                     /gene="bglX"
                     /locus_tag="JN755_003767"
     CDS             complement(1023..3320)
                     /gene="bglX"
                     /locus_tag="JN755_003767"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7382396.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            3516..5231
                     /gene="dld"
                     /locus_tag="JN755_003768"
     CDS             3516..5231
                     /gene="dld"
                     /locus_tag="JN755_003768"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7382397.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(5269..>6096)
                     /gene="pbpG"
                     /locus_tag="JN755_003769"
     CDS             complement(5269..>6096)
                     /gene="pbpG"
                     /locus_tag="JN755_003769"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7382398.1"
                     /translation="PEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLD
                     ARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYP
                     GGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLS
                     TTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPV
                     ALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
BASE COUNT         1416 a         1651 c         1539 g         1490 t
ORIGIN      
        1 atactccttc aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc
       61 ttgcggatcg cttaaggttt gcagccccag cgccgcgacc ggagcgtcag tgccgtaagc
      121 cattgcggca ttaacgccag aggtttgctg ggcagcggct ttaatcgtca ccgccgtgtc
      181 gccaccagcc agtgacagca actgatcctg accgagctta aagccatagg ctttttcaaa
      241 cgcgggtaac gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc
      301 gccctcttgc agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac
      361 atcctgacgc acggcgatag tccaggtgtt atttgcaggc gcgggcgtca gccagattaa
      421 cttgttgtgc tccgcatcga gctttttgac tttctcgtag ccctgcccgg catttttcca
      481 cgctgcatcg ttttcatctt taaagaagaa agcgccattg ccggtatatt ccggatagat
      541 atccagttca ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt
      601 attcaccgtc ggtacgccgt ggctttcgag tacctgcaag atgatgttgc cgagcagcgc
      661 gccttcggta tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcagcaggct
      721 cacggctgcc aacataacca gtgaacctgc ccagagcttt aagagtggca tgatgctttc
      781 ctcattcttt tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga
      841 gttagcctga gcggaaggat tcagttgcag aatcagataa atacttaaga ggcatattcg
      901 gtccggcgtt tcctgccgga tgcggcgcga gcgccttatc cggcctacaa agggcgaaaa
      961 ttcagcaagt taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag
     1021 cattacagca actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaataca
     1081 ttgaacttgc caggctcggc gtcatatttc atctgttgat tccagaactt cagcgcctca
     1141 atatcgatcg ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag
     1201 cctttcagct gtttcacagg gcgactcatg gaagccgtca catcctgcaa gtacatctgc
     1261 actaccgtgg ccccctcgcg cttaccggtg ttcgtcacct gcacgctggc cgtcactttg
     1321 ccgtcacgct tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag
     1381 ctcagaccat agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg
     1441 tatttgttcg gcttgtcggc attatacgga cgaccggtgt tcagatggct gtagtacacc
     1501 gggatctgcc cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg
     1561 ccaaacaata catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga
     1621 atcgcatcag cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc
     1681 agcaccagcg gtttaccggt ggctttcagc gccgcaatca agtcacgttg gccttgcgga
     1741 atagtgatat cggtccggct ggaggcttcg tgcgccatcc cctgtgcttc accgactaca
     1801 gccaccacca catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc
     1861 gaacgcggat cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg
     1921 tcactggtaa cgttcgcccc tttggcatac agcactttac cgttttcacc gacggaattt
     1981 ttaatcccgg tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc
     2041 atcacgtcac gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc
     2101 agcggtaacg tttcgagacg gtttttcagc aacactaggc tttcgcgcgc cacttcacgc
     2161 gcttctttac ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc
     2221 cccaaatggc tgtatgggtc gttaaacaac cccatatcat atttaacgtt cagtacatgg
     2281 cgggcagcat cgtccagctc tgccatggtc actttgccgg atttaatcaa cccaggcaga
     2341 tacttcgagt agtactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc
     2401 accgcatctt ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga
     2461 tcggaaacgg tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag
     2521 gaatcggagg tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg
     2581 cctgcgtcca gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc
     2641 atatcgacgg tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg
     2701 acgctggtca tcaccgagta acgatctgcc gggcttttac cctgcatcgc ttccaccatg
     2761 gttttaccca ttattgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc
     2821 cagcgcggat cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct
     2881 gccgcttcat aagcagagac acgcccgacc gttttcaccg catcgaggtt aaaagacgag
     2941 gccagaccga ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag
     3001 aaaagaggaa ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc
     3061 tgacgggtta cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc
     3121 gcctctttcg gattatccgg gcctacgctg attaagcgca gctgaccaat tttctcatca
     3181 accatcattt tcttgagcag atcggtgacg aacgcatccc gcgcttcagg cgttaatgga
     3241 tggttaccga ataaatcatc cgccagtgct ggctgcagga ccagactcac cgcgattcct
     3301 actgaacata gccatttcat atggattttt cttcctttgt tgcccgacgt ggcagcgaaa
     3361 atggtgcaaa aaccgtagtt tgccataagc atgatggaga gagaaaaaga atactcagtt
     3421 tattgtctga attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt
     3481 ttgtgatatt ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata
     3541 ataaagcctt tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc
     3601 ccgcaaaaac ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg
     3661 tcgttttccc tggctcacta ctggaattgt ggcgggtgct gaaagcctgc gtcaccgccg
     3721 acaaaattat tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg
     3781 gtaacgacta tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg
     3841 ttctcggcaa gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa
     3901 aagccctcaa accgctggga cgcgaaccgc attcggtgat tggatcatcg tgtataggcg
     3961 catcggtcat cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg
     4021 cgtataccga aatgtcgtta ttcgcgcgta tagatgaaga tggcaaactg acgctggtga
     4081 accatctggg gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgacg
     4141 atcgcatcaa agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg
     4201 tccaccgcgt tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatccggatc
     4261 ggttatttga atcttctggt tgcgccggga agctggccgt ctttgcggta cgtcttgata
     4321 ccttcgaagc ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc
     4381 tgaccgaaat ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat
     4441 atatgcaccg ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga
     4501 ttgataagtt aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg
     4561 cgatgctgga gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgca atgcaaaaat
     4621 tcggtcacct gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg
     4681 agcatcatct gctgttaaaa atggcaggcg atggcgtcgg tgaagccaaa tcgtggctgg
     4741 tggattattt caaacaggcc gagggcgatt tctttgtctg tacgccggag gaaggtagca
     4801 aagcgttttt acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt
     4861 ccgatgaagt cgaagacatt ctggcactgg atatcgctct gcggcgtaac gacaccgagt
     4921 ggtatgagca tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc
     4981 attttatgtg ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg
     5041 cgttaaaaga acagatgctg gaactgctac agcagcgcgg cgcgcagtac cctgccgagc
     5101 ataacgtcgg tcatttgtat aaagcaccgg agacgttgca gaagttctat cgcgagaacg
     5161 atccgaccaa tagcatgaat ccggggatcg gtaaaaccag taaacggaaa aactggcagg
     5221 aagtggagta aaaattacgg atggcagagt atcgccatcc gaattcactt aatcgttctg
     5281 tgccgtctgc cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc
     5341 tgccggcaca ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc
     5401 aaaatgggta tatttgccaa acgcgtccat cactaccagc gccaccggtt tattattgat
     5461 aaccgtacgc atcaccagac aatggcccgc cgcattggta aagccggttt tggttaactg
     5521 aatattccag ttatcgcgat acaccagatg attagtattg cggaacggca gcgtatacgt
     5581 cgggttagag aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata
     5641 ttgtttgctg gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga
     5701 caatccggta ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt
     5761 cattgcctta ataaaggctt tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc
     5821 gcggttttct gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt
     5881 cagtcgtacg cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag
     5941 tttttcatcc agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat
     6001 agacgcaatc ggacgcacca gatccgggtg gttcgaatag atcactttgt tggtattcag
     6061 atcaacaatc atcgcgctac cggaggcaat ttccgg
//
LOCUS       DADLTI010000203         6081 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLTI010000203 DADLTI010000000
VERSION     DADLTI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.205"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            142..762
                     /locus_tag="JN755_003770"
     CDS             142..762
                     /locus_tag="JN755_003770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="FKBP-type; rotamase; catalyzes the cis-trans
                     isomerization of proline imidic peptide bonds in
                     oligopeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7382399.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1070..2482
                     /gene="cycA"
                     /locus_tag="JN755_003771"
                     /gene_synonym="dagA"
     CDS             1070..2482
                     /gene="cycA"
                     /locus_tag="JN755_003771"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7382400.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2527..3189)
                     /gene="ytfE"
                     /locus_tag="JN755_003772"
     CDS             complement(2527..3189)
                     /gene="ytfE"
                     /locus_tag="JN755_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7382401.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3297..4262)
                     /locus_tag="JN755_003773"
     CDS             complement(3297..4262)
                     /locus_tag="JN755_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7382402.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(4371..5231)
                     /gene="qorB"
                     /locus_tag="JN755_003774"
     CDS             complement(4371..5231)
                     /gene="qorB"
                     /locus_tag="JN755_003774"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7382403.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5320..5700
                     /locus_tag="JN755_003775"
     CDS             5320..5700
                     /locus_tag="JN755_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7382404.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5826..>6081)
                     /locus_tag="JN755_003776"
     CDS             complement(5826..>6081)
                     /locus_tag="JN755_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382405.1"
                     /translation="PDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIR
                     FETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
BASE COUNT         1365 a         1539 c         1638 g         1539 t
ORIGIN      
        1 cgcctttatt atattcatac gggatattgc tattgtttct ttttccccgg gatttgtcat
       61 cattcctgcg acaaagtttt acccaaagaa gtgtggctga tatgctgccc gctactttta
      121 taccctaaga aaggaaatac gatgaccacc ccaacttttg acaccatcga agcgcaagca
      181 agctacggca ttggtttgca ggtagggcaa caactgagtg aatctggcct ggaagggctg
      241 ctgccagaag cactgattgc aggtattgcc gatgcgctgg aaggcaaaca tccggctgtg
      301 ccggttgatg tggtgcatcg cgcgctgcgt gaaatccacg aacgcgccga tgccgttcgt
      361 cgtcagcgtt tccaggcgat ggctgctgaa ggtgtgaaat acctggaaga aaacgccaaa
      421 aaagaaggtg tgaatagcac cgaatctggc ctgcaattcc gcgtgatcaa ccaaggtgaa
      481 ggcgcaattc cggcacgtac cgaccgcgtt cgtgttcatt acaccggtaa actgatcgac
      541 ggcaccgtgt ttgacagctc cgttgctcgt ggtgaacccg ctgaattccc ggttaatggc
      601 gtgatccctg gctggattga agcactgact ctgatgccgg taggttctaa atgggaactg
      661 actatcccgc aggaactggc atatggcgag cgcggcgcag gtgcatccat ccctccgttc
      721 agcaccctgg tgtttgaagt cgaactgctg gaaatcctct aagcagcgca ttctgttccc
      781 ctcgaaccag aggggagcag gcattcagca ataaaccctt cagtttgcca aacggcgcta
      841 ttttgtgttg caaagacccc gtaagcgtgt atttttgtga gctgtttcgc gttatcaccg
      901 tgatatgaca ctcactttaa acataaaatt aacattagat ctaaatctta gtattcatcc
      961 cgcgtattgt tacctaatat cgatgagtcc cgatacagat tcgtcgtatc atagactgac
     1021 taaaggccgt agagcctgaa caacacagac aggtacagga agaaaaaaca tggtagatca
     1081 ggtaaaagtc gttgccgatg atcaggctcc ggctgaacag tcgctacggc gcaatctcac
     1141 aaaccgacat attcagctta ttgccattgg cggtgccatt ggtactgggt tgtttatggg
     1201 gtccggcaaa acgattagcc ttgccgggcc gtcgatcatt ttcgtttata tgatcattgg
     1261 ttttatgctc tttttcgtga tgcgggcaat gggggaattg ctgctttcga atctggaata
     1321 caaatctttt agtgacttcg cttccgattt actggggccg tgggcgggat atttcaccgg
     1381 ctggacttac tggttctgct gggttgtaac cggtatggca gacgtggtgg cgatcacggc
     1441 ttatgctcag ttctggttcc ccgatttctc cgactgggtc gcctcgctgg cggtgatagt
     1501 gctgctgctg acgctcaacc ttgccaccgt gaaaatgttc ggtgagatgg agttctggtt
     1561 tgcgatgatc aaaatcgtcg ccatcgtgtc gctgattgtc gtcggcctgg tcatggtggc
     1621 gatgcacttt cagtcaccga ccggtgtgga agcgtcattc gcgcatttgt ggaatgacgg
     1681 cggctggttc ccgaaaggtt taagtggctt ctttgccgga ttccagatag cggttttcgc
     1741 tttcgtgggg attgagctgg taggtacaac agctgcggaa accaaagatc cggagaaatc
     1801 actgccacgc gcgattaact ccattccgat ccgtatcatt atgttctacg tcttcgcgct
     1861 gattgtgatt atgtccgtga caccgtggag ttcggtagtc ccggagaaaa gcccgttcgt
     1921 tgagctgttt gtgttggtag gtttgcctgc ggctgccagc gtgatcaact ttgtggtgct
     1981 gacctctgcg gcgtcttccg ctaacagcgg tgtcttctct accagccgta tgctgtttgg
     2041 tctggcgcag gaaggtgttg cgccgaaagc gttcgctaaa ctttctaagc gcgcagtacc
     2101 cgcgaaaggg ttgaccttct cgtgtatctg tctgctcggc ggcgtggtga tgttgtatgt
     2161 gaatcctagc gtgattggcg cgttcacgat gattacaacc gtttccgcga ttctgtttat
     2221 gttcgtctgg acgattatcc tttgctcgta cctggtgtac cgcaaacagc gtcctcatct
     2281 gcatgaaaag tcgatctaca agatgccgct cggcaagctg atgtgctggg tatgtatggc
     2341 gttctttgtg ttcgtggtcg tgttgctgac actggaagat gacactcgcc aggcgctgct
     2401 ggttaccccg ctgtggttta tcgcgctggg gttgggctgg ctgtttattg gtaagaagcg
     2461 ggctgctgaa ctgcggaaat aaccgcatta tcatgctgga tggcgcaatg ccatccagct
     2521 tttagatcac tcacccgcta gggcgcgtgg gaacagtaca ttgttttcca gactgatgtg
     2581 atccatcagg tcatcaatca gttcattaat gccgttatac atcgctttcc aggtggtgca
     2641 ggcttctggc ggcggtgtga cgttattggt agtgtgtttg atcacttcta gcagttcgcc
     2701 cgcttcatcg tgctcgcttt ccattacgct gattggcccc attgcctggc tgcccatgcc
     2761 ttgtttgatc atcgggaaga ggatctgctc ttctttcatc atgtggctgg aaagctcttc
     2821 atgcagcatg gtcaggtatt ttgtcagccc ttttggcacg ctcggtttgt cggcgtgaac
     2881 gcgctcgact ttagtcgctt gcaggatcag ctccggcagc tgctcgcggt gacgatcgtg
     2941 gtagcgcacg atgatatggt caatgatttc tgccagcggg gcgctacgcc agtctttctc
     3001 aatcggttgt tcagcaagct ttgccagttc agcttcaatg acctcaacat ccagttcttt
     3061 acgtgccgcc gcgcgcgcca gcgtctgctt accgccacag cagtaatcca tatcatattt
     3121 acgaaacaga gccgaagcgc gagggataga gagcgccagt tcacctaaag gttggtcgcg
     3181 ataagccata gctgatacct cattcttaat aagataagat gtattttaaa tacaccttta
     3241 aggcaaaagc tataaccctt acgtagcaga ggcttattta actcactgcc agcatgtcaa
     3301 ctttctgaga cagattcagt taaaggtttt tctggttttg ctctgaccgc gatgaccacg
     3361 ccaatcacta acagcgcgat accgctcagc gtcattagcg gcggcatttg ctggcggagt
     3421 aaaaaggtgt acagcaaacc tgccagcgtt tcgaaaacaa tcagcggccc gagaatcact
     3481 gtcggtaatc gctggctggc gacgttccag cagagtgcgc caacccatga gcaaagcacg
     3541 gctatcgcaa ccatcagact aataaacacc agcggacggg ggccaaaagg taaggagaag
     3601 tccggcgttt gtatattcag ccagtaacag gcgacgagat agccgatgag agaaaccggc
     3661 agcgtgacca gcgcctgcgc cgtcgcccac atcatcggat gtttgtcggg attttcccgc
     3721 agccagcggg cgttgcgcag ggcataccat gcccagcaga ccacggaaac taacgctagc
     3781 acgatgccag aggtataacg tgcccagtca aaatcgggga gtccgtggtt taactcagca
     3841 atattcacac tcgccaggcc gatgccaata caaatcagtg ccggggcgag ttttccccac
     3901 gcgagtttgc cgtcgcgctg gctataaagc agattggcaa agacaggaat caccaccggc
     3961 agggtgccga taatcatcgt ggaaacaggc gcgccagtac gttgaatggc actggcaagg
     4021 cagaagtaat aaatgaggtt gcccatcata gtgagcatca aggcggtaag ccagtcccga
     4081 cgcgccaact gacgcagacg cacgcgtccc agccaggcaa tgggtagcgc aattaaccct
     4141 aacgccagat aacgccccat cgactgtaac atcgccgggt attccggcac gatcaacggc
     4201 ccgacaaaaa taagccccca catcaaccct gctaacaggg cgtacagcac gccgctaatc
     4261 attactggca tccattggtc tgtcagaaga aacgttcagg aagcagacat gattgctccc
     4321 ggcatcccgt cattatcaga gaggatgcgg gaggatgcca ctttaattaa ctagttatta
     4381 acattaaaaa gatggcttac gctttcggct aacgttgtcg ttgggcggcc aatcaatttg
     4441 ctaagcgttt tgctgttatc aaacagacca cctttcgatg cgccaacgtc agaatccgcc
     4501 agcatatccg ccagtccgtc gggcagtccg acgcttttca gtgccgcggc gaaatcggct
     4561 tcgctcagat tttgataggt aacctgtttg ccgctctgtt tggtcagctc tgccgctaac
     4621 tgtgtcaacg tccaggcact atcgcccgcc agttcgtaaa ccttgccttc gtgaccggct
     4681 tcgctaatca cgagtgccgc agctgtcgca taatctgccc gcgttgctga ggcgattttg
     4741 ccatcgcccg ccgcaccgat aaatacgccg tgttccagtg ctgccggggc gctggcgagg
     4801 tagttttcgc tgtaccagcc gttgcgcagc agggtgtaaa cgatgccaga atcagccagc
     4861 attttctccg tctcgatgtg ctcatcggcg aggccgagcg gggaggtatc tgcatgtagc
     4921 aggctggtat aagcgataaa tttcacgcca gccgcctttg cggcattaat aacattacga
     4981 tgctgcgggg cacgttgacc cacttcgctg gaagagatca gcagtagttt ttccactccc
     5041 tgaagtgcag atgtcagtgc ggcttcatcg ctgtagtcag cctgacgcac ggtaatgcct
     5101 tgtgctgcca gggcctgggc ttttgccgga ttacgaacga tagccactat ttggctggca
     5161 ggaaccgttt tcatcaagga ttcaataata tagtgaccaa gttggccagt ggcaccagta
     5221 atagcgatca tgggaagtct ccatcgtttt gtcttgtgtt taaatacgct aacacctaaa
     5281 cttactttta gtaagtacgt gcaaaaaggt aagtatgaaa tgagtcaggt tagcctgtca
     5341 caacaactga aagagggtaa tctctttgcg gaacagtgcc cgtcgcgcga ggtgttgaaa
     5401 cacgtcacca gccgttgggg ggtgttgatt ctggtggcgc tacgcgaagg tactcatcgc
     5461 tttagcgacc tgcggcgcaa aatcggtggg gtgagtgaaa agatgcttgc gcagtcgtta
     5521 caggcgttag aacaggatgg ttttcttaac cgtatcgcgt atccggtggt gccgccgcat
     5581 gtggaatata gcctcacgcc gctgggcgag caggtgagcg aaaaggttgc tgcactggcg
     5641 gactggattg agttgaattt gcccgaggtg ttggcggtgc gggatgaacg tgcggcataa
     5701 ctgccggatg cgctgcgctt atccggtcta cggttcatgg cttgtagggt ggataagata
     5761 cggcagcatc gcatccgcca ataaatgccg gatgcggttg atttataggc cgaaaaagaa
     5821 gtgttttact tactcaaatc cacctgataa atcgcaaacc cgatatcatc ggtcgcgact
     5881 ttattcatcg gatactgccc tttctcttta ataaacgctg cggctttatc tgacggggaa
     5941 gtttcgaaac ggatatccag tttcttatcg gcagctatcg gtgctaaacg ccagttgtta
     6001 tctgccgccg gatgaatttc ccccgcacgt ttcgactcat cagcaatcca cgctgccagc
     6061 accgagcggt tctcatccgg t
//
LOCUS       DADLTI010000204         6078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLTI010000204 DADLTI010000000
VERSION     DADLTI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.206"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            60..461
                     /locus_tag="JN755_003777"
     CDS             60..461
                     /locus_tag="JN755_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382406.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            421..630
                     /locus_tag="JN755_003778"
     CDS             421..630
                     /locus_tag="JN755_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HAY7382407.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            623..1345
                     /locus_tag="JN755_003779"
     CDS             623..1345
                     /locus_tag="JN755_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7382408.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLATAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            1345..1635
                     /locus_tag="JN755_003780"
     CDS             1345..1635
                     /locus_tag="JN755_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7382409.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1632..1994
                     /locus_tag="JN755_003781"
     CDS             1632..1994
                     /locus_tag="JN755_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7382410.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            1991..2179
                     /locus_tag="JN755_003782"
     CDS             1991..2179
                     /locus_tag="JN755_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7382411.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            2176..2799
                     /locus_tag="JN755_003783"
     CDS             2176..2799
                     /locus_tag="JN755_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7382412.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3068..3286)
                     /locus_tag="JN755_003784"
     CDS             complement(3068..3286)
                     /locus_tag="JN755_003784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382413.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            3511..3834
                     /locus_tag="JN755_003785"
     CDS             3511..3834
                     /locus_tag="JN755_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY7382414.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3818..4294
                     /locus_tag="JN755_003786"
     CDS             3818..4294
                     /locus_tag="JN755_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY7382415.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            4291..4758
                     /locus_tag="JN755_003787"
     CDS             4291..4758
                     /locus_tag="JN755_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7382416.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            4746..4898
                     /locus_tag="JN755_003788"
     CDS             4746..4898
                     /locus_tag="JN755_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382417.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            5104..5304
                     /locus_tag="JN755_003789"
     CDS             5104..5304
                     /locus_tag="JN755_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7382418.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            5277..5609
                     /locus_tag="JN755_003790"
     CDS             5277..5609
                     /locus_tag="JN755_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7382419.1"
                     /translation="MEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEFEIEVYEVF
                     KTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEV
                     QMYLPGMV"
     gene            5866..5985
                     /locus_tag="JN755_003791"
                     /pseudo
     CDS             5866..5985
                     /locus_tag="JN755_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
BASE COUNT         1762 a         1379 c         1558 g         1379 t
ORIGIN      
        1 ttctcaggaa gtttagtctc caggattccc ggggcggttc atcgtgcgag gaaatgacaa
       61 tgcttttaat tcaacctgga tttggcctta gcatcaaaaa agggcacatg tttggcgaga
      121 aagagtctca acgaaaaatg gtgtcgatac agttgccatt tatcagtatt tattggctaa
      181 acagggaggc aacaaattat tggtatacct gcgccagagc agcatttaac gaccctgact
      241 ggtttgtgaa aaaccaccac gcagttcgtc aggcaaagag aaaggccaat acgacataca
      301 tgaaggcgta tcgaaaagca tggaaagaac accgcgatcg ataccaacaa gacatggaaa
      361 agcttgaatc agaaaacatg gaattaagac gaaagctcgg tgaagcaaaa cgagacatcg
      421 atgcttacaa gcgacttttt aatggtgaaa gccatgctta gcccatccca atcccttcaa
      481 taccagaaag aaagcgtcga gcgagcttta acgtgcgcta attgcggtca gaagctgcat
      541 gtgctggaag ttcacgtgtg tgagcactgc tgcgcagaac tgatgagcga tccgaatagc
      601 tcaatgtacg aggaagaaga cgatgaatga gttaataaat ggcaatgcca tcaaaatgac
      661 aagcattgaa atcgctgagt tggttggtaa gcgtcatgac aatgtgaaac gtaccatcga
      721 aacgctggct aaaaatggtg ttatccggct tcctcaaatt gaggattgtg gaagaatcaa
      781 tgggttaggc ttaaatcaaa gtttttgtgt gtatgtgttc gaaggcgaac aaggaaagcg
      841 agacagtatt gtcgttgtag cccagttgtc gccggaattc accgctcgcc ttgttgaccg
      901 ctggcgagag cttgaagaag ctgcggttaa tatccccaaa acgctaccgg aagcgttgcg
      961 ccttgctgct gatcttgctg agcagaaaat gcaactggaa aaccagctcg caactgccgc
     1021 acctaaagtt gagtttgccg atcgcgttgg cgaggccagc ggaattttga ttggaaactt
     1081 tgcaaaggtt gttggaattg gtccaaacaa actgtttgcg tggatgcgcg atcacaaaat
     1141 ccttattgct tcaggttccc ggcgcaatgt gccaatgcag gaatatatgg atcgcggcta
     1201 tttcacagtg aaagaaacag cggtcaacac aaatcacgga atacagatat cgttcaccac
     1261 aaaaatcacc gggcgtggtc aacagtggct gaccagaaag ctgctcgata atggaatgct
     1321 gaaagtaaca ggggaggctg cttaatggct aatctacgca aagaagcacg cggcagagaa
     1381 tgccaggtac gtatttacgg cgtatgcaat ggtaatcctg aaactacagt tctggcacat
     1441 taccggatgg ctggaatttg cggaacggga atgaagcctg acgacctgat cggcgcatgg
     1501 gcttgtagcg cgtgtcacga tgaaatcgac cgacgcaccc ataatctcga caacaaagac
     1561 gccagacttt accacctcga aggcgtgatc aggacgcagg cgatactgct gaaggagggg
     1621 aagattaagt catgagcgaa tatcagtttg tgcttccata cccgccgtcg gtgaacacct
     1681 actggcgaag acggggaaac caatactaca tcagcgataa aggccagaaa taccgaaaag
     1741 aagttcagca aataatcctc caactcaagt tagacatttt caccaaatca cgactccgca
     1801 tcaaagtcat cgcagacgtt ccagactccc gccgccgcga cctcgataac atcctgaaag
     1861 gtttactcga ctcccttatc cacgccggat ttgcggaaga cgacgagcaa ttcgatgaca
     1921 ttcgcgtaat tcgtggtgtg aaagtaccag gcggaaggct tggaataaaa atcaccgaac
     1981 tggagaacgt atgaacgcca caattcaaac gataccagag cttcttatcc agacacgagg
     2041 caatcagacc gaagtggcga ggatgctttc ctgcgcaaga ggaacagtgc tcaagtacaa
     2101 ccgagacagc aaaggcgagc gtcacgtaat agttaacggc gtcctgatgg tcaaacaggg
     2161 caagagggga agacgatgag actcgaaagc gtagctaaat ttcattcgcc aaaaagcccg
     2221 atgatgagcg actcaccacg ggccacggct tctgactctc tttccggtac tgatgtgatg
     2281 gctgctatgg ggatggcgca atcacaagcc ggattcggaa tggctgcatt ctgtggtaag
     2341 cacgaactca gccagaacga caaacaaaag gctatcaact atctgatgca atttgcacac
     2401 aaggtatcgg ggaaataccg tggcgttgca aagcttgaag gaaatactaa ggcaaaggta
     2461 ctgcaagtgc tcgcaacatt cgcttatgcg gattattgcc gtagtgccgc gacgccgggc
     2521 gcaagatgca gagattgcca cggtacaggc cgtgcggttg atattgccaa aacagagctg
     2581 tgggggagag ttgttgagaa agagtgtgga agatgcaaag gcgtcggcta ttcaaggatg
     2641 ccagcaagcg ccgcatatcg cgctgtaacg atgctaatcc ccaaccttac ccaacccaca
     2701 tggtcacgca ctgttaagcc gctgtatgac gctctggtgg tgcaatgcca caaggaagag
     2761 tcaatcgcag acaatatttt gaatgcggtc acacgttagc agcatgattg ccacggatgg
     2821 caacatatta acggcatgat attgacttat tgaataaagt tgggtaaatt tgacccaacg
     2881 atgggttaat tcgctcgttg tggtagtgag atgaaaagag gcggcgctta ctaccgattc
     2941 cgcctagttg gtcacttcga cgtatcgtct ggaactccaa ccatcgcagg ctgagaggtc
     3001 tgcaaaatgc aatcccgaaa cagttcgcag gtaatagtta gagcctgcat aacggtttcg
     3061 ggatttttta tatctgcaca acaggtaaga gcattgagcc gataatcgtg aagagtcggc
     3121 gagcctggtt agccagtgct ctttccgttg tggtgaatgc acaggctgat gtgtaagggc
     3181 aagaatcttt cgctggattc ggtgtggcca cgtagccagc tgtaggcagt tgcagcaaac
     3241 cggagatcag caccggtcgc cacaatccaa actgagccgt agccactgac tatcctgaat
     3301 tcatcagtga tagttacgct gcggccttct acacatgatc ttcgtgaaag tgggcggcat
     3361 gaggttgcgc taacaaccta atgccgtttt gcccgtgcat atcggtcacg aacaaatctg
     3421 attactaaac acagtagcct ggatttgttc tatcagtaat cgaccttatt cctaattaaa
     3481 tagagcaaat ccccttattg ggggtaagac atgaagatgc cagaaaaaca tgacctgtta
     3541 gccgccattc tcgcggcaaa ggaacaaggc atcggggcaa tccttgcgtt tgcaatggcg
     3601 taccttcgcg gcagatataa tggcggtgcg tttacaaaaa cagtaatcga cgcaacgatg
     3661 tgcgccatca tcgcctggtt cattcgtgac cttctcgact tcgccggact aagtagcaat
     3721 ctcgcttata taacgagcgt gttcatcggc tacatcggta ctgactcgat tggttcgctt
     3781 atcaaacgct tcgctgctaa aaaagccgga gtagaagatg gtggaaatca ataatcaacg
     3841 taaggcgttc ctcgatatgc tggcgtggtc agagggaact gataacggac gtcagaaaac
     3901 cagaaatcat ggttatgacg tcattgtagg cggagagcta tttactgatt actccgatca
     3961 tcctcgcaaa cttgtcacac taaacccaaa actaaaatca acagccgccg gacgttatca
     4021 gcttctttcc cgttggtggg atgcttaccg taagcagctt ggcctgaaag acttctctcc
     4081 gaaaagccag gacgctgtgg cactgcaaca gattaaagaa cgtggcgctt taccgatgat
     4141 tgatcgcggt gatattcgtc aggcaattga ccgttgcagc aatatctggg cttcacttcc
     4201 gggcgctggt tatggtcagt tcgagcataa ggctgacagc ctgattgcaa aattcaaaga
     4261 agcaggcgga acggtcagag agattgatgt atgagcagag tcaccgcgat tatctccgct
     4321 ctggttatct gcatcatcgt ctgcctgtca tgggcggtta atcattaccg tgataacgcc
     4381 atgacctaca aagagcagcg agataaaaaa gtcagtgagc tgaagcaggc gaccgccacc
     4441 attactgaca tgcagcaacg ccagcgtgct gctgatgcac tcgatgctaa atacacgaag
     4501 gagttagctg atgcgaaagc tgaaaatgat gctcttcggc gcaagcttga taatggtggc
     4561 agggtgctcg tcaaaggaaa atgccctgtg ccatcctcag ccgaaacctc cagcgcctcc
     4621 ggcatgggca atgatgccac cgtcgaactc tctccagttg ctggacgaaa cgttctcggt
     4681 atccgggacg gaattatccg cgaccaaaca gcactgagaa cgcttcagga atacatcagg
     4741 acgcaatgcc ttcgatgata gcgataattt tactcatcat ccttcacatc tggctctgta
     4801 gacagggggg tgatcacttc tggagtaaat ccagattaaa catctcattg ctgatgcttg
     4861 atattgagca tctggcgcgc ggtaaggggc tgcgttgaga taagagccag tcatcacaaa
     4921 caccaggatt tagcctcgca ttcgcggggc ttttttatat ctgcaacaaa cgcgcttcac
     4981 acgcgcgact tatgaacaca gagcctttca ggatgaccct tgaggatgcc ggtttggtaa
     5041 tcggtgcctt tctgtgggcc ggaatcctgt gtgacaaggt tcatcactaa aaggtaatca
     5101 ctgatgaagt acccaacagt tattgtcaat ggtgtgtccg ttcgtgttga tgaggatgga
     5161 cgctacaact taaacgatct ccatgcagca gcagttgcaa atggagaggc tacagagcaa
     5221 cagcgcccaa gccagttttt gcgtagcgcg cagataaaac gcttcataaa agcactttgg
     5281 aacaaattca accacttaaa ataatcaaag gtggtgcaga accaggtgtg tggggtgttg
     5341 aacttctggc aatcagatat gcagcatgga ttaagccgga atttgaaatc gaagtttatg
     5401 aagttttcaa aacggttgtc cgtctcggcg ttggcgcaat gtcccgtctg aatagaatcg
     5461 atcacatcat caatactgaa accaaagcga taagccagtg cgcaagccaa atggctaagt
     5521 ggggcgttgg tgggcgaaaa agattgcttc atgttgcacg tgagagagcg gcaaatgaag
     5581 tgcaaatgta tttgcccgga atggtgtgat ttcgcaggtt aatccagttt ttgcattacg
     5641 gcagtaccgc gaaacaaccc aagccagtaa gtggggaaat aacactggca gccactgaaa
     5701 gatgaacctc cagccttatg gcaaaaaaga ttctttgtgg tggcggactg atggaaagac
     5761 atcggttatt gcagaggcca ttcaatgagt ggtctcgata atggcttata ccctacacgg
     5821 gataacttaa ctgatatccc ttttaacgga taaacggagc caacaatggc agagattatt
     5881 cccatgactg aagaacagaa attccagtta gagatttaca aactggttat gaaccagaac
     5941 gcagccgcag aagaagcatt tcagttcatt ggcactgatg aagtggcaca ctgaatttgg
     6001 ccacctgaac agaggtgata tgctcacctc agaacaacac aggtgctcca atgaaaaaaa
     6061 gaaattttag cgcagagt
//
LOCUS       DADLTI010000205         6009 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLTI010000205 DADLTI010000000
VERSION     DADLTI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.207"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            292..888
                     /gene="wrbA"
                     /locus_tag="JN755_003792"
     CDS             292..888
                     /gene="wrbA"
                     /locus_tag="JN755_003792"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7382420.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            909..1136
                     /locus_tag="JN755_003793"
     CDS             909..1136
                     /locus_tag="JN755_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382421.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN755_003794"
     CDS             complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN755_003794"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY7382422.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2708..3964)
                     /locus_tag="JN755_003795"
     CDS             complement(2708..3964)
                     /locus_tag="JN755_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7382423.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            4225..5145
                     /gene="cbpA"
                     /locus_tag="JN755_003796"
     CDS             4225..5145
                     /gene="cbpA"
                     /locus_tag="JN755_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7382424.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            5145..5450
                     /gene="cbpM"
                     /locus_tag="JN755_003797"
     CDS             5145..5450
                     /gene="cbpM"
                     /locus_tag="JN755_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7382425.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(5543..5959)
                     /gene="torD"
                     /locus_tag="JN755_003798"
                     /pseudo
     CDS             complement(5543..5959)
                     /gene="torD"
                     /locus_tag="JN755_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         1610 a         1431 c         1360 g         1608 t
ORIGIN      
        1 ggactaagct tagtgaactg acgcgggcag acccgcgttt ggtaatatcc tgcaacagct
       61 tcttagcgag aatagatatc atctccaata attatccata agccgctctt atgattaaaa
      121 aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt
      181 tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt atctttaaat
      241 aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga aatggctaaa
      301 gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct
      361 gagggtgcaa gcaaagtgga tggtgcagaa gttgtcgtta agcgtgtacc ggaaaccatg
      421 ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg
      481 caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg
      541 tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta
      601 tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaacc
      661 atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac
      721 gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc
      781 accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt
      841 tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga
      901 ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc
      961 gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa
     1021 tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt agtcaaagcc tttgctaaga
     1081 ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag
     1141 cgactccccc gcttctcgcg ggggagtttt ctgttatttc accgcctcat tcaacacgct
     1201 atcaaactta tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag
     1261 cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc
     1321 actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg
     1381 ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg
     1441 tttgaaatcc agcgccgtta acagagaggc tatgttggag tcgtgcccaa ctaacaccgt
     1501 gattttcgga gcactggcgc gatcggtgac cagggctttg tcgatataac tgaccagcgg
     1561 tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga ctgtcctggt aaccgttttt
     1621 cagcttcgac aataccttcc actgctggtc agatttgatt tctccccagg ccacctgatc
     1681 catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt tgccgacttt
     1741 cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc
     1801 caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt
     1861 ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc
     1921 caccgcctgt tcactgaatg cggcggaatc atcggtgatc atcgggttaa aggttgggtc
     1981 catagtgccc attttttcct ggtgatgcac aggaatatca cacccaggga atgcaccagt
     2041 aataaagaac tgggcggtag cgacggtacg ttgcagacta ttggcatagg cataaacggt
     2101 gtccggcggc gggcattccc ccgatttcac tatcccctgc tctgccagcc attcacgcat
     2161 gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac
     2221 gtcccattcg ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag
     2281 cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg
     2341 tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat
     2401 tagcgttttg ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa
     2461 atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat
     2521 atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg
     2581 cgtacgccgc tgataattgc acctgtggct aaacagaata tctgcggcgt taccaataca
     2641 gagacaatat ttaccaataa aaaagtgtga gactaataac aatggttttt agcatatgcc
     2701 tttgccatta agttgcataa cgatttaaat agtcaagttg cgcattcatt tccttatttt
     2761 ttaacttata agcctgagaa aaaagttcag gagggaggta aacatcataa tgattgccag
     2821 tgtttattaa ataaccatac tcaagatctt catctgttga aaaggattca tttcccaagg
     2881 agattgatgc cacattacta ttaacaataa ataatgagca aatgctatct gaaagaacag
     2941 caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc ggtatctaaa gcaagcaaat
     3001 tcttgttatc attaaatata aactcgggta aatgcagtat gcattttttg tcattcaagc
     3061 aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacataaaa ctacccacac
     3121 cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtctttaca tcttccctga
     3181 gattatagaa tgccgtatca acaagagcct cgatcgtttg tttatcaaca attttaataa
     3241 actcttcgta tacttgcttt tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt
     3301 cttttaaaac cagtatggct cttaacagac aatatccatc gccatctact ggagcgtatt
     3361 ttaatccata tgttttatta atttcactct gaataaaaga tatgccagac aaatcaatac
     3421 cagtatctaa atcagatatt ctcggcatat ttatctgttc tggctggata ctcatatttt
     3481 catcatctaa acaatactca gaaaaaacat catcacacgc attaatgttt tcgatatatt
     3541 ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa cctctgcccg
     3601 gcaaggacac tctaacaata tcaacatctt gaacatattg tatagtatat gtaatacctc
     3661 ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata aataatgata
     3721 gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcacat tccagacatg
     3781 gagtatcctc ttcatttact ttacgtctga gttcattgta caaccgcata aaacacacca
     3841 gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtactca ttttttatat
     3901 cgttccaggc ctgtttcaaa taattatttg cagtgcaact cattccatgt atattactac
     3961 ccatatatag cgtctataaa atttaataaa taatgacgcc ctagttaaac ttaaagtgcc
     4021 tggttcaact atcaaaaatc gctcaccctt tttcacctgt ttaaaatatg ttcagcaacc
     4081 catcttgatg gcgacctcct ctccgcgatg atttcaataa catattctgt gttggcatat
     4141 gaaattttga ggattaccct acacttatag gagttacctt acaggggttc cttcaatttg
     4201 tgttgattta cgcgagataa cgctatggaa ttaaaggatt attacgccat catgggcgtg
     4261 aaaccgacgg acgatctcaa gacaatcaag accgcctatc gtcgactggc ccgtaaatac
     4321 catcctgatg tcagcaaaga accggatgcc gaagcccgct tcaaagaggt cgctgaagcc
     4381 tgggaagtgt taagtgatga acaacgtcgc gctgagtatg atcagatgtg gcaacatcgc
     4441 aacgatccgc aatttaaccg tcagttccat catggcgacg gtcagagttt taatgccgaa
     4501 gattttgacg atatcttctc gtcaattttc ggtcagcatg cccgccagag ccgtcaacgc
     4561 cccgccgcac gcggccacga tattgaaatc gaagtggcgg tattcttcga agaaacgctt
     4621 actgagcata agcgtaccat cagctataac ctgccggttt ataacgcctt tggcatgatc
     4681 gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg caggcgtcgg caatggtcaa
     4741 cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg gcggtccaaa tggcgatttg
     4801 tggctggtga ttcatattgc gccacatccg ctgttcgata ttgtcggcca ggatctggaa
     4861 attgtggtgc cggttagccc gtgggaagcg gcgctgggta ctaaagtcac cgttccaaca
     4921 ctgaaagaaa gcattttgct gactatcccg ccaggcagcc aggccgggca acgattgcgc
     4981 gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg atctgtatgc ggtactgaaa
     5041 atcgtgatgc cgccgaaacc ggatgaaaac actgccgcgc tgtggcagca actggcagac
     5101 gcccagtcgt cttttgatcc acgtaaagat tgggggaaag cataatggct aatgttacgg
     5161 tgacttttac tattaccgaa ttttgcctgc ataccggcat ctctgaagag gagttgaatg
     5221 aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca ggaaacaacc tgggtatttg
     5281 acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct gcgtcatgaa ctggctctgg
     5341 actggccggg gatcgcggtg gcgctgacgt taatggatga tattgcgcac ctgaagcagg
     5401 aaaaccgcct gctacgccag cggctttccc ggtttgtagc tcatccgtga tgtgtgtttg
     5461 ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc tgatgcgacg ctaaacgcgt
     5521 cttttcaggc gcataaacga cgttatctgt tttggtggtc gcactccact aacaccagca
     5581 ataactggct tagtgccgcg taaaaaccaa agctgtcata ctgtcgacaa cgcgcagcaa
     5641 actctggtaa ccattgccgt agcgccgtca gtattttttg ccgcaaactg tcgattcttc
     5701 gcgcaggaac ggtcccctct cccagcgaaa aatgcagata actcaacaac tcgagataga
     5761 tcgccagatg atctgccggt tcgttgaaat tgccactggt ttccatccct gcctcaacta
     5821 acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga tgcatacggc agcgccgctt
     5881 gtttgtcggt catcagaaac aggccgcaaa agtcagcggc cagttccaga cgggcatcgt
     5941 cacgtactgt cagcgtggca ggtaatgctg ccaacttact gatttagtgt atgatggtga
     6001 ttttaaggt
//
LOCUS       DADLTI010000206         6002 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLTI010000206 DADLTI010000000
VERSION     DADLTI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.208"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..93)
                     /locus_tag="JN755_003799"
     CDS             complement(<1..93)
                     /locus_tag="JN755_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382426.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(346..1695)
                     /gene="lysC"
                     /locus_tag="JN755_003800"
     CDS             complement(346..1695)
                     /gene="lysC"
                     /locus_tag="JN755_003800"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7382427.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2220..3869
                     /gene="pgi"
                     /locus_tag="JN755_003801"
     CDS             2220..3869
                     /gene="pgi"
                     /locus_tag="JN755_003801"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7382428.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4331..4573
                     /locus_tag="JN755_003802"
     CDS             4331..4573
                     /locus_tag="JN755_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382429.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4687..5325
                     /locus_tag="JN755_003803"
     CDS             4687..5325
                     /locus_tag="JN755_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7382430.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5322..>6002
                     /locus_tag="JN755_003804"
     CDS             5322..>6002
                     /locus_tag="JN755_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7382431.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSET"
BASE COUNT         1501 a         1565 c         1542 g         1394 t
ORIGIN      
        1 accatgcgca atccgcgccc gatccagcag tgttttaagt tcacgctgct cgcgttcagt
       61 catctctttt tgggtaatac gggggagtgc catgatggtg ccctcagtga gccagagagt
      121 tcagtgtacg ggaattcgtt tgcgagcaga actgataata ttcattttgc cttctcttaa
      181 ctatttatct ctttcgataa ttaattagag atgcagataa aaaaattaaa ggcaattatt
      241 ctccgataaa gacgttggtt aacagagtac aggctcgttt attgatgagc atagtgacaa
      301 gaaaatcaat acggcccgaa atatagcttc caggccatac agtatttact caaacaaatt
      361 actatgcagt ttttgcacca cctgctcggc atcttcgccg ggcaccagga agcacaggtt
      421 atggctggat gcgccgtaac aaatcatgcg aatgttgaac ggttccagta cgccgaatac
      481 ctctttgcca acgccgcagg cttttgacag gtcattgcca atcaacgcga ccagcgccag
      541 accttcttcc acctccaccc ggcacagtgc ggaaagctcc atcagcagag attgcgtcag
      601 caacgtatcg ccagtggagg ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc
      661 tgacgtggtg attaagtcta ccgaaatatt atgccgcgcg aggatgccga aaacttccgc
      721 gaggaagccg cgagaatgca gcatattcag gctgtgcaaa gtgagcagag tctgattgcg
      781 acgaagcgcc agcgcgcgga acagcggcgg attttcagtt ttattgcaca ccagcgtacc
      841 acctgcgcgt gggtctttgc tggagccgac aaagaccggg atatcgctgc gtactgcggg
      901 tagcaacgtt gccggatgca gtacttttgc accaaaagtt gccatctctg ccgcttctgc
      961 aaacgcgatt tcatcaatgc gttttgctgc ggaaactacg cgtggatcgg tggtgtagat
     1021 gcccgggacg tcggtccaga tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc
     1081 tgccgtataa tcgctgcctc cacggccaag cgtcgttgta cgacctttat tttcgctacc
     1141 gataaatccc tgggtgatca ctaagccttc attgaggcgt gggagcagct gcagcgcggc
     1201 cagttccgcc agcgcggcta catctggctc tgcacgacca aatcggtcgt tggtacgcat
     1261 cactttacgt acatcaaacc actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa
     1321 cagcagggtc gacatcagct cgccgtggct gaccagctca tctgtcagcg ccggagacgt
     1381 tgccagcgcc gccgcttctg ccagaacagt aatgttctcc agcagacgtt caatctcttc
     1441 acggataacg ttcgggtaac gcagacgttc cagaatggca aactggatgt tgcgaatagc
     1501 gtcgagtttt tcgaatcgct cgccaggttc cagcccttcc gctaaagcga ccagcagatt
     1561 agtgatacca gcagaagccg agaggactac taaacgcacg ttggcatcag aaagcacaat
     1621 atcagcgctg cggttcatgg cgtcaaaatc agctacgctg gtaccgccaa atttggagac
     1681 aacaatttca gacataacta cctcatgtca ggggatccat tttcagcctt ggcacaaggg
     1741 aagagcggaa gacgggtggg cgcagagcga tacttcgcta ctattttcac ccagaagtgc
     1801 tccaccactt gcgaaacgcc cgactgcgaa cgcttctggt gacaacccag gggattcagc
     1861 ccctgtagcc gatgatgaac gtggccagcc gttcaatcac ctcggcgatg caccccctca
     1921 ggtgttatca caggactggc tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc
     1981 ctgcgctagc gcaggtagta catttataaa taaagggtga gcggggcggt tgtcaacgac
     2041 ggggtcatgc ggatttttca tccactcctg gcggtcagta gttcagctaa taaatgcttc
     2101 actgcgctaa gggtttacac tcaacattac actaacggca ctaaaaccat cacatttttc
     2161 tgtgactggc gctacaatct tccaaagtca caattctcaa aatcagaaga gtattgctaa
     2221 tgaaaaacat caatccaacg cagaccgctg cctggcaggc actacagaaa cacttcgatg
     2281 aaatgaaaga cgttacgatc gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt
     2341 tctccgcaac cttcgacgat cagatgctgg tggattactc caaaaaccgc atcactgaag
     2401 agacgctggc gaaattacag gatctggcga aagagtgcga tctggcgggc gcgattaagt
     2461 cgatgttctc tggcgagaag atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc
     2521 tgcgtaatcg tagcaatacc ccgattctgg ttgatggcaa agatgttatg ccagaagtca
     2581 acgcggtgct ggagaagatg aaaaccttct cggaagcgat tatctccggt gagtggaagg
     2641 gttataccgg caaagcaatc actgacgtag tgaacatcgg gatcggcggt tctgacctcg
     2701 gcccgtacat ggtgaccgaa gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg
     2761 tttctaacgt cgatgggact cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca
     2821 cgctgttctt ggtagcatct aaaaccttca ccactcagga aaccatgacc aacgcccata
     2881 gcgcgcgtga ctggttcctg aaagcggcag gtgatgaaaa acacgttgcg aaacattttg
     2941 cggcgctctc aaccaatgcc aaagccgttg gcgagtttgg tattgatact gccaacatgt
     3001 tcgagttctg ggactgggtc ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga
     3061 ttgttctctc catcggcttt gataacttcg ttgaactgct ttctggcgca cacgcaatgg
     3121 acaagcattt ctccaccacg cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg
     3181 gcatctggta caacaatttc tttggtgcgg aaactgaagc gattctgccg tatgaccagt
     3241 atatgcaccg tttcgcggcg tacttccagc agggcaatat ggagtccaac ggtaagtatg
     3301 ttgaccgtaa cggtaacgtt gtggattacc agactggccc gattatctgg ggtgaaccag
     3361 gcactaacgg tcagcacgcg ttctaccagc tgatccacca gggaaccaaa atggtaccgt
     3421 gcgatttcat cgccccggct atcacccata acccgctctc tgatcaccac cagaaactgc
     3481 tgtctaactt cttcgcccag accgaagcgc tggcgtttgg taaatcccgc gaagtggttg
     3541 agcaggaata tcgcgatcag ggtaaagatc cggcaacgct tgactacgtg gtgccgttca
     3601 aagtgttcga aggtaaccgc ccgaccaact ccatcctgct gcgcgaaatc actccgttca
     3661 gcctgggtgc gttgattgcg ctgtatgagc acaaaatctt tactcagggc gtgatcctga
     3721 acatcttcac cttcgaccag tggggcgtgg agctgggtaa acaactggca aaccgtattc
     3781 tgccagagct gaaagatgat aaagaaatca gcagccacga tagctcgacc aatggtctga
     3841 ttaaccgcta taaagcgtgg cgcggttaat catcatgcgt gcctgatgcc gatatgtagg
     3901 ccggataagg cgtttacgcc gcatccggca accggagcct gatgccaata tcgacattaa
     3961 tgcatagtat gaggagttaa ataaccttaa aaacaatgcc gacaataagt cggcattgtt
     4021 ttatcagata aatccccttg tctgtgattt aacggaaatc ataccgtgag gttaatccta
     4081 aaatagattt ttaatcgttg tttatttcgg agaatacgca gattaattac ttttgttttt
     4141 attttaagtt tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt
     4201 cattccaatt atcctaaaac tccatcgcta attccccgcg ccgtaattcg catgctttag
     4261 ttgtgtatac tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag
     4321 ggaaattgtt atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac
     4381 ttctgcgtgg gctgcacctg tacaggtggg cgaagcggca gagtcggcag caacgtcggt
     4441 ttcggcgggg agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc
     4501 gctcgcggta accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac
     4561 cagtacccag taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt
     4621 gaagtatgaa gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga
     4681 agagttgtga agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc
     4741 acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag
     4801 ctgacggacg atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc
     4861 gggccgcaat tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact
     4921 caggatcaag ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc
     4981 gacaacctga ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt
     5041 gttgatggtg caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac
     5101 gccaccgcac gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat
     5161 gaaaccctgg ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac
     5221 cgctactgga tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat
     5281 tatttcccgg tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg
     5341 tcgcgttgct tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt
     5401 tcagcaatgg cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg
     5461 tagggcaacc tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg
     5521 caacggcggc agcattgcgt cagcagcagg cgttgctgac acggctggca gagcagggcg
     5581 cagattccag caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat
     5641 tgaaagtgac aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac
     5701 gcggtaaccc gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg
     5761 tcacgttgtt cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg
     5821 tggcgagcta tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg
     5881 tcgtttaccc ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg
     5941 tagagccgat gcccggcagc attatttatg ttggcctcgc agattccgtc tggagtgaga
     6001 ct
//
LOCUS       DADLTI010000207         5997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLTI010000207 DADLTI010000000
VERSION     DADLTI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.209"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..268)
                     /locus_tag="JN755_003805"
     CDS             complement(<1..268)
                     /locus_tag="JN755_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7382432.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JN755_003806"
     CDS             805..2706
                     /locus_tag="JN755_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY7382433.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JN755_003807"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JN755_003807"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7382434.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JN755_003808"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JN755_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7382435.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JN755_003809"
     CDS             complement(4548..4757)
                     /locus_tag="JN755_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382436.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JN755_003810"
     CDS             5041..5403
                     /locus_tag="JN755_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7382437.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1567 a         1454 c         1488 g         1488 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gagcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgc
//
LOCUS       DADLTI010000208         5975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLTI010000208 DADLTI010000000
VERSION     DADLTI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.210"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            85..315
                     /locus_tag="JN755_003811"
                     /pseudo
     CDS             85..315
                     /locus_tag="JN755_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JN755_003812"
     CDS             complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JN755_003812"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7382438.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYAQQKLSLAS"
     gene            2279..2605
                     /gene="glpE"
                     /locus_tag="JN755_003813"
     CDS             2279..2605
                     /gene="glpE"
                     /locus_tag="JN755_003813"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7382439.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2650..3480
                     /gene="glpG"
                     /locus_tag="JN755_003814"
     CDS             2650..3480
                     /gene="glpG"
                     /locus_tag="JN755_003814"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7382440.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3497..4255
                     /locus_tag="JN755_003815"
     CDS             3497..4255
                     /locus_tag="JN755_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7382441.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4237..5835)
                     /locus_tag="JN755_003816"
     CDS             complement(4237..5835)
                     /locus_tag="JN755_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7382442.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1356 a         1558 c         1594 g         1467 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 gtataatttc gccattcaga aagaatggaa agaataatgc aaaatagaaa atggattttg
      121 acctcgctgg taatgacttt tttcggcatc ccaatactga cgcaattttt tgcagcggtt
      181 attgccatgc tgggggctgg gcttgccgct attcctgagg tttgcaattt actctttacg
      241 ccaacaattt accttctgct caatattttt atgctgacgc tgggcgcatt aatgctattt
      301 ttctcggggc gagtggggaa cgtgcctgag ttttgttacg ttgggtatga tggcgtgggc
      361 tttgcggtaa ttccctgaat taaaaagtag gttagatttt tgacaaagtg cgctttgttc
      421 atgccggatg cgacgtgaac gtcttatctg gcctacaaga ccatgcaaat tcaatatatt
      481 gcataaacca cgtaggcctg ataagcgtag cgcatcaggc agttttgtgt ttgtcatcag
      541 tctcaggcca gattgaaatc tgacctgatc accttacgtt aatttacgac gccagcgaca
      601 acttctgctg cgcatattcc accagccact gactcacacg agattgttga tccgcattca
      661 gccacatgcc ttgttttgtg cgacgccaca gggcatcgtc ggtgcggcgg acccattcgt
      721 gatccaccag gtatttcagt tccgcttcgt agaactcatg accgaaatct tccccgagat
      781 cgcttaccgt tcccgcattg ccgagcagca gctcgctgtt gctgccgtaa gtgcgcgcgt
      841 agtgacgtgc cagcgattca gtcaggaacg ggtaacggcg gcgcaggcga gcggcgtaat
      901 cgtcgcggtc gccttcaatg gcaccacccg gcagcacgct ctctttcgtc cacgccgggc
      961 caataccctg ataatacggc gtcagttttt ccagcgcatg ttctgccagt tttcggtagg
     1021 tggtcagctt accgccgaat accgacagca gcggtgcttt gccgttttca tcatggatat
     1081 caagggtgta atcacgggta atggcttgcg gcgagtcgga ctcatcatca cacagcggac
     1141 gcacaccgga gtaggtccag acgatatcgt cacggcttag ctgcttttta aagtgcgcgt
     1201 tatacacttt cagcaggtaa ttgatttcac tctcttcaat cttcactgct ttcggatcac
     1261 ctttgtattc cacatcggtg gtgccgatga tggaaaactc gtccatccac gggatcacga
     1321 acacaatgcg tttatcttcg ttttgcagaa tgtaggcctg cttttgggta tgcacgcgcg
     1381 gcaccacaat atggctgcct ttgatcaggc gaatgccata aggcgaaggc agatgcatcc
     1441 cctcgtcgaa gaactgtttc acccacgggc cggtggcgtt aaccaggccg cgcgcttgcc
     1501 agctatattt tttgccggta tcgatatctt ccgcttccac aatccacagg ccgttttcgc
     1561 ggcgagcaga ggtggcgcga gtccgagtaa gcacttcgcc gcctttacgc accaccatct
     1621 gggcgttggc gagtaccaga cgggcgtcgt ctacccaaca gtcagaatat tcgaatccgc
     1681 gcttaatttc cggttttaac actgaatttg cgccaaaacg caaaccaatt gatcccggca
     1741 ggctggtgcg tttacccaga tgatcgtaca taaacagacc aatgcgaatc atccacgccg
     1801 ggcgcagatg cggacgatgt ggcaggcgaa aacgcatcgg gaaggcgata tgcggagcca
     1861 ttttcagcag cacttcacgt tcagccagcg cctcgctgac caggcggaat tcatagtgct
     1921 caaggtagcg caggccaccg tgaatgagtt ttgaactggc ggaagaggtc gcgcaagcga
     1981 gatcctgcgc ctccagcatc agcacggata aaccgcgtcc agcggcgtct gccgcgatac
     2041 cagcaccatt gatgccgcca cctatcacaa tcagatcttt ggtttccatg ctgccctcat
     2101 tcactttcgt taaagctcat aaatgttcgt tatcgaacat attagcaaag aatcgcgctt
     2161 tgggtaacat tgaaaaaaca ttttagagtg atatgtataa cattatggcg tttatctgcc
     2221 gcttcgacgt aaactgtgcg gtaaatttgc ccacttgttt gtaaagaaag agagacgcat
     2281 ggatcagttc gaatgtatta acgttgccga cgcgcaccag aagttgcagg aaaaagaggc
     2341 ggtgctggtc gatattcgcg atccacagag tttcgctatg gggcatgcgg tgcaggcttt
     2401 ccatttaacc aacgacacgc tgggcgcgtt tatgcgtgat aacgactttg acactccggt
     2461 gatggtgatg tgttatcacg gcaatagcag caaaggggcg gcgcagtatc tgctgcaaca
     2521 gggctacgat gtggtctata gcattgacgg cggctttgaa gcctggcagc gacagtttcc
     2581 cgcagaggtg gcgtacggcg cgtaacgctt tatactgtcc ccttttgtgt ggaataagcg
     2641 acagcaacga tgttgatgat tacctctttt gctaaccccc gcgtggcgca ggcgtttgtt
     2701 gattacatgg cgacgcaggg tgttatcctc acgattcaac aacataacca aagcgatgtc
     2761 tggctggcgg atgagtccca ggccgagcgc gtgcgggcgg agctggcgcg ttttctcgaa
     2821 aacccggcag atccgcgtta tctggcggcc agctggctgg caggccatac cggcagtggc
     2881 ctgcattatc gccgttatcc tttctttgct gccttgcgtg aacgcgcagg tccggtgacc
     2941 tgggtgatga tgatcgcctg cgtggtggtg tttattgcca tgcaaattct cggcgatcag
     3001 gaagtgatgt tatggctggc ctggccattc gatccaacac tgaaatttga gttctggcgt
     3061 tacttcaccc acgcgttaat gcacttctcg ctgatgcata tcctctttaa cctgctttgg
     3121 tggtggtatc tcggcggcgc ggtggaaaaa cgcctcggta gcggtaagct aattgtcatt
     3181 acgcttatca gcgccctgtt aagcggctat gtgcagcaaa aattcagcgg gccgtggttt
     3241 ggcgggcttt ctggcgtggt gtatgcgctg atgggctacg tctggctacg tggcgaacgc
     3301 gatccgcaaa gtggcattta cctgcaacgt gggttaatta tctttgcgct gatctggatt
     3361 gtcgccggat ggtttgattt gtttgggatg tcgatggcga acggagcaca catcgccgga
     3421 ttagccgtgg gtttagcgat ggcttttgtt gattcgctca atgcgcgaaa acgaaaataa
     3481 ttccagggat ttataaatga aacaaacaca acgtcacaac ggtattatcg aactggttaa
     3541 acagcagggt tatgtcagta ccgaggaact ggtggagcac ttctccgtca gcccgcagac
     3601 tattcgccgc gacctcaacg agctggcaga gcaaaacctg atcctgcgcc atcatggcgg
     3661 cgcggcgctg ccttccagtt cggttaacac gccgtggcac gatcgtaaag ccacccagac
     3721 cgaagaaaaa gagcgcatcg cccgcaaagt ggcggagcaa atccccaatg gctcgacgct
     3781 gtttatcgat atcggcacca cgccggaagc ggtggcgcac gcgctgctca atcacagcaa
     3841 tttgcgcatt gtcaccaaca atctcaacgt tgctaacacg ttgatggtaa aagaagattt
     3901 ccgcattatt ctcgccggtg gcgaattacg tagccgcgat ggcgggatca ttggcgaagc
     3961 gacgctcgat tttatctccc agttccgcct tgatttcggc attctgggga tcagcggcat
     4021 cgacagcgac ggctcgctgc tggagttcga ttaccacgaa gttcgtacca agcgcgccat
     4081 tattgagaac tcgcgccacg ttatgctggt ggttgaccat tcaaaatttg gccgtaatgc
     4141 gatggtcaat atggggagca tcagcatggt tgatgccgtc tacaccgacg ccccgccgcc
     4201 agtaagcgtg atgcaggtgc tgacggacca ccatattcaa ctggagctgt gctgatcctg
     4261 cacggcctcc cacgtcagac caaaacgcgc caggtatttg cgtagccgat ccgcgtcatt
     4321 gacgctggct ttgccctggc gcgaaacatc aaaaagctgt cgtccggcag cagaaagcga
     4381 ctttgcctgg cggcagatag cgataacgtg ttccagttgc atacggtcga agagatcgat
     4441 gttttctgcc tctgcgccca gcaacgccgt aagcgcggag gggcggctct cctgccagtt
     4501 atagcgcaga cggtttatct catcttccac cacgtccaga gtgatgcgtc cgctagtggc
     4561 aaaggtggcc atccgcgtga cgctggcaga aagttcgcga aagttaccgc gccatgtagc
     4621 ctggggagat gttgcaaagg ccagccaggc acgccgcgct tcggtgttaa aacgcacgct
     4681 gtcaccagtg agtgtggcgt ggcgctccac ttcataatcc aggttcggtt caatatcttc
     4741 ctggcgctgg cgtagacccg gcagggtgaa ggtccagaga ttgatccgcg cgtacagatc
     4801 ttcgcggaat ttgccttcgg caaccagctg gcgcaaatcg cgcaccgttc cggcgataag
     4861 ctgaaaatca ctgctcacct ggcgatcgct gccaaacggg taaaaggttt tctcttcaat
     4921 agctttcagc agcattgcct gttcgtctgc gcccagttcg ccaatctcat caagaaacaa
     4981 cattccgccg ttggcgctgc gtaataatcc ttcacgagat tcccgcgccc cggtaaacgc
     5041 gcctttcaca tgaccaaaca gcgtcgacat cgcggtatcg ccgcgcaggg tggcgcagtt
     5101 cacttccaca aacgcgccgc taaactgatg ccgtgcctgt tttaactcga agatgcgtcg
     5161 cgccagaaat gacttacctg cgccggtcgg gccgttaagc agaatcggcg cgcgggattt
     5221 aatcgccact ttttcgatct gctcaatcat gcggttgaag tgggggttac gcgtggcaat
     5281 accggatttg aggaaatcga gcgtttgctg ccgttcctcg gcaaagcggc tggcgatggc
     5341 gttataacgg cttaaatcga gatcgataat cgtcacttca cctgggccgc acggctgttc
     5401 ttttttgcgc ggagttgaag attgtatcaa ccgggcgggt agataacgcg cttccgccag
     5461 cagaaaccag caaatctgcg cgacgtgggt gccggtggtg atgtgaatta aatagtcttc
     5521 tttttctggc tgaaactcgt aaccacgggc gaaatcatgc aggcaggcgt aaacctcttc
     5581 gaaatcccac gggttatgca gttcaatctc aacgcccacc acttctgttt ctggcgaaac
     5641 gctggcgata tcgcgtttaa gcgtttcaaa tagcgagcgc gagcgggcat cgtgcaataa
     5701 ttccagtcga tcgatgacca acgattcttg ctggcatata cagagtgacg gacgccattt
     5761 tgaccagcgc tgactgccgc gtccggcata atccagcacg gtaccgacaa agccaaaagc
     5821 cactgtttta cgcatcttag atatccttat aaaaggcgat atttcattat cggtaaatcg
     5881 gctttgcggg aaatctccgt ttacggacat aaagatataa tctttttaga aacaataaat
     5941 taaaaaatta atctgcttat ttaattttct ggcac
//
LOCUS       DADLTI010000209         5954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLTI010000209 DADLTI010000000
VERSION     DADLTI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.211"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1110
                     /locus_tag="JN755_003817"
     CDS             <1..1110
                     /locus_tag="JN755_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7382443.1"
                     /translation="WPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMA
                     VAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQ
                     ALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRD
                     PFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVF
                     IDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFID
                     ARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHD
                     QPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1122..1934)
                     /locus_tag="JN755_003818"
     CDS             complement(1122..1934)
                     /locus_tag="JN755_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382444.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1938..2723)
                     /locus_tag="JN755_003819"
                     /pseudo
     CDS             complement(1938..2723)
                     /locus_tag="JN755_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2720..3388)
                     /locus_tag="JN755_003820"
     CDS             complement(2720..3388)
                     /locus_tag="JN755_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7382445.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3452..4090)
                     /locus_tag="JN755_003821"
                     /pseudo
     CDS             complement(3452..4090)
                     /locus_tag="JN755_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4103..5954)
                     /locus_tag="JN755_003822"
                     /pseudo
     CDS             complement(4103..5954)
                     /locus_tag="JN755_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT         1511 a         1597 c         1369 g         1477 t
ORIGIN      
        1 tggcctgatg aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg
       61 gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat
      121 gcggcaatgg cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc
      181 gtttttcacc gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt
      241 attttacccg aagccgattt ctattgccct attccctgcg agcccttaca catcgtcacc
      301 gatcaggcat taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt
      361 agattgatag tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta
      421 gagagcctga atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac
      481 ccatttgact gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat
      541 actgttccct ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct
      601 catctgaatg aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat
      661 aattatgcgg caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg
      721 atcattcata ttctcgccct cggtgaagac tacgaaatgg aaattaatga gtcgcgcacc
      781 gtcctgaaaa cggaagacaa cagctactcg tttgacgttt ttattgatgc ccgcggacag
      841 cgtccgctta aagtgaaaga tattcctttc cctgggctac gcgagcaatt acagaaaaca
      901 ggggatgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
      961 ggacgcgtag cgttcggcgc gttgccctgg ctgatgcacg accagccttt cgttcaggga
     1021 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
     1081 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
     1141 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
     1201 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg
     1261 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
     1321 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
     1381 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
     1441 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
     1501 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
     1561 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
     1621 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
     1681 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
     1741 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
     1801 tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg
     1861 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
     1921 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
     1981 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
     2041 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
     2101 acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac
     2161 atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg
     2221 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
     2281 cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg
     2341 atcagcacaa agccgagcca gcatgccagt aacaaaaatc agcaaacttc atgcaccgca
     2401 accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc
     2461 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa
     2521 agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag
     2581 gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
     2641 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
     2701 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
     2761 gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca
     2821 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg
     2881 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
     2941 tatctgccac tttagtgaca gggttaaggt agcgaacctg gtacggacaa gcgccaatgc
     3001 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
     3061 acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
     3121 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
     3181 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
     3241 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
     3301 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
     3361 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
     3421 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
     3481 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
     3541 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
     3601 cggcggtcgc ggcttcaatc tgcgcttcct agaagtaata tggcgcttgc atcagcatgg
     3661 cgactttgta aggacgagga tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc
     3721 gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct
     3781 gcgttgagta atagtaatca tcctgacttc ccgtcataca agcgatatcc gcgtagtctc
     3841 cgttctgctt aatgctttcc agcaaaactg acagttcctc ttctgcaacc gaatagggtg
     3901 gcgagagaaa cacggtgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact
     3961 cagccaggat ttcagcaggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct
     4021 gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatcct
     4081 gatggttcat cttacccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg
     4141 caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt
     4201 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
     4261 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga
     4321 taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat
     4381 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg
     4441 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
     4501 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
     4561 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
     4621 tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt
     4681 tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga
     4741 acatacagtt aacaatgaaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
     4801 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc
     4861 agcgttctgc gatggcatat gaaccataag ccaggtgact cagctcaccc acacgatgcg
     4921 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
     4981 gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat
     5041 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
     5101 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
     5161 cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg
     5221 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
     5281 agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg
     5341 atttataggt acgaaagccg accgtatttt gattgcctgg cggaatttca cccgtttcaa
     5401 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg
     5461 gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac
     5521 caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt
     5581 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc
     5641 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt
     5701 tttccccagc ctgaccaata gccgccacac aggtttcccg actggtcaat cgacaaattt
     5761 cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
     5821 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
     5881 cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgcgactaa
     5941 atttcctttg gtaa
//
LOCUS       DADLTI010000210         5905 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLTI010000210 DADLTI010000000
VERSION     DADLTI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.212"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(220..588)
                     /locus_tag="JN755_003823"
     CDS             complement(220..588)
                     /locus_tag="JN755_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382446.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(1082..2682)
                     /locus_tag="JN755_003824"
                     /pseudo
     CDS             complement(1082..2682)
                     /locus_tag="JN755_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2740..3337
                     /locus_tag="JN755_003825"
                     /pseudo
     CDS             2740..3337
                     /locus_tag="JN755_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            3340..5610
                     /locus_tag="JN755_003826"
                     /pseudo
     CDS             3340..5610
                     /locus_tag="JN755_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            5621..>5905
                     /locus_tag="JN755_003827"
     CDS             5621..>5905
                     /locus_tag="JN755_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7382447.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT         1571 a         1459 c         1504 g         1371 t
ORIGIN      
        1 acttaagcca atgtagtgag tagacgagct actaactgag atgactgaag aacgctaacg
       61 agctggaggt cgatacacga ggtctgacag aggcggtcac atgccttgat tacttaggag
      121 agatacttaa tgtcggttga ccagatgctc gcatgttctg acgcatcaga ctcagaggcg
      181 gaggctggtc tccctatagt gatagtttag ggtccgtaat catgaaccag ctttactggc
      241 gtggtactgg acggatttac tggcgctgac gacggtagca tagacgtagc gagcgtagcg
      301 agttgaaacc ataggttcac ccaaggcaac acgcttacgg ctcagggact cctgcacagc
      361 gcttacagtt aatgtgtcgt gcgttagtag ttgggtcaga atcaagtatg ggctgacagg
      421 cttggtattg cttgccattg attcgttcag gcgaccagca ccagtaagga catcatccag
      481 ccaaccactc aggtcgccct gaccatcgaa tgacagcagg tccccgcagt gctgctgcaa
      541 gtaggctttg gtgaacgtgt tgtcgtgagt tgcgattggt cgtgccatag agtatctcct
      601 gtagttgaat tagttggttc ccctatagtg ataatcaagt ccatcactgg tgatgcatac
      661 agtagcgcga caggatgtga ctcacaggct ggactggatg tcacggatta aggcgaccct
      721 aggaattaag gaagtaaata taaagagggg gaccggatgt gactcacagg ttgaaccaaa
      781 tgtcaaggac taaagacgcc tcaggaatta aggaagtata tataaagaga gaccagaggc
      841 gactcacagt taaacaggaa tgtcaagagg agaccatagg aatttggagg gactggagtt
      901 taggacgcag tttaaataag accacagaca gacttagagt cggacagccg aaggcggacc
      961 agtgggccta tagtcaggag ggattatgat tattatcacc ctccctaact tcagtcagac
     1021 ttcagtgacc caaggtctca accatcaagc cgtatatggt ctccaccttg ggtctcccta
     1081 cttaaagtaa agctgtctta agcacatcag ccagcctacc cacagcgtgt cctttcccgt
     1141 acaggtcata cgtaattgac tgactgctgt gacctaagat gccttgagcg tccaccagat
     1201 tgactccacg ctctttcatt cgggtcgcca gccaatgtct cagactgtgg agagagacat
     1261 cctgagagtc tcctagggcc tcagtcagga cccttgagtt aaaccagcta gagtaggcgc
     1321 tcggagtcat cccgaagaga ggaccatcat caggctgcat gtctacccat ttgaggaaag
     1381 actcaaggtc gaacccatga acgccatcgg tcagcggcac aagtcggact gaatgcttgt
     1441 tcttcacaga cttaccgtcc ccatcttcgt ttatatcgat acagaccagt ccattgtcta
     1501 aggttacaat gtcacgttta gtcaggtggc agacctcagc agaccgagcg ccagtaacta
     1561 cagaaaccag agacgcccat ctacgggcct cagaggtcgt gtgagccgtg tgtcgtgtga
     1621 actgatactc agcctcaaca cggaccagaa gtctctccag ctcctcgtct gtgaacgctc
     1681 tgcgcttgga gtcgatatcc ttggtcagct tcatccgttc aatagggttg cggccctcaa
     1741 gttgcccatt catcagaccg taatcaagac acatcttcgc cttagtcagc agcttgttaa
     1801 tcgtcgacgg tctcacctca gaagccagca tagcgtccct gacagccagc cactcagagc
     1861 gggacatcag gtccatgtct tcaggtaaga accgagagac ggtattcagt gaggacgaca
     1921 ggtccttgta gctggatgac ttcagggtct gggccttctc agccagcagc gaggacacta
     1981 ggcttgaaaa aactacagat ggttggcgct cctgtgtgaa aactgctggc tatccccctg
     2041 ctcattcttc agaattgacg tagagtcgcc tatagttgag taatcagtat ggttattatc
     2101 atcaatcagc ttaatcagac cagaggtatc accagtgtct accctctgct gtgctgcggt
     2161 caggacttca agcgctaacc ttatgccctt ctgctggtcc agactcaagg cttgcgtagc
     2221 ggctagctcc ctcaggttgg acttctcgtc caccaaagta gcgacctcaa gtccattcca
     2281 gtaatcgtca ctagcgtctg tcaggaactg ctcagccata gaccggagat gctctgtaag
     2341 ctcctgtagt gagacttcag gcctatccaa catgaaggcc ttagcggtgg ctgcaagctc
     2401 tctctgacgc attgtggcta ctctcctgtc tgtgctgcgg gtcgatactg aaagaaacgg
     2461 tgtggtctgt cccttgagtc tgaagcggat agtatatcga ccgttagctc tacggactaa
     2521 ctgtggcgta gcgagccttg cgagcaagtg cgggactgca aggtccttga tttgtgtcag
     2581 ccttgtgtca ggacttgagg tgtagacttg ctgatacaga tagggagacc cagagaaaga
     2641 actggatgca tatacagtat tatcatggtt aatgtcagtc atatcaggac cttaagtagc
     2701 gttgagtcaa ctacttcaag tccatccatc cgtctgacag tggagtccat ctggaactac
     2761 gcgggaaata ccctcgttaa tcagcattct gacatcaaca aaacgcatga aattctgcag
     2821 gacgaatcga aatgcgaaat gattgtggtc atcgaaaact ttatgacctc atacggcaaa
     2881 atatgccgac attctgctgg cagacctgat gaccgttgag caggaagata ttattcctaa
     2941 cgactacgcc ggtaacatgg gatatctcat tttcctccag ccagtcacca gcgaaaaatt
     3001 cgaacgtaaa ccgatttact ggatcctgag tgaagtcgcg aaacgtcttg gacctgacgt
     3061 ctatcaaaag ttcacagaag gtcgcacgca ggaacaatgg ttacaacatc tgtacgccaa
     3121 aatgcttgcc aaagatccgg cgttaccgtc ttacgacgaa ctgaaaaaaa tgggtatcta
     3181 taagcgtaaa gatcccaatg gccattttgt cgcctacaaa gcatttcgtg acgaccccga
     3241 ggcaaatcca cttaaaacgc cttccggtaa gattgaaatt tattccagca agctggcgga
     3301 aattgcccgt acctgggaac tggaaaaaga tgggtagaag agaaaacggt ctggagttcc
     3361 tgcaccgtta actgcgggag ccgctgtctg ctacgtttgc atgtgaaaga tgacaccgtg
     3421 tactgggtgg agtctgatac gacaggtgac gacgtttacg gtaatcatca ggttcgggca
     3481 tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc ctgacaggtt gaaatatccc
     3541 atgaagcgcg tcggcgagcg cggtgaaggt aaatttgaac ggataagttg ggacgaagcc
     3601 ctggatacca tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta
     3661 catgtcctgt acggaacagg cgtagatggc ggaaatatca ccaactcgaa cgtcccgtac
     3721 cgtctgatga actcttgcgg gggttttctc agtcgctatg gcagctacag taccgcacag
     3781 atcagtgccg caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc
     3841 gccaatacga aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggc
     3901 ggtggtgtca cttactacgt cgagcaagcc ctcgaacgtt caaacgcacg catgatcgtc
     3961 atcgatccac gctataacga cacagctgcc gggcgtgaag atgaatggct gcccattcgc
     4021 cctggcaccg atggcgcact ggcctgtgcg attgcctggg tactgattac tgaaaacatg
     4081 gtcgatcagc catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc
     4141 aacgcaccac gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata
     4201 gctaaaacgc cggaatgggc agcaaaaatc accagcatcc cggcagaaaa aattatccag
     4261 ttggcacgag agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa
     4321 cgacactcca acggcgaaca aacgtcccgc gctattgcca tgctttccgt tcttaccggt
     4381 aacgtcggca taaacggcgg caactcaggt gtacgcaaag gtagctggga tctgggggta
     4441 gaatggttcc cgatgctcga gaatcctgtt aaaacgcaga tttccgtttt tacatggaca
     4501 gatgccatcg accatggtac gaaaatgacc gcgacccgtg atggtgttcg tggaaaagaa
     4561 aaactggatg tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa
     4621 catggcgaca tcaatcacac ccatgaggtg cttcaggatg acagcaagtg cgagatgatt
     4681 gttggcattg accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcctgac
     4741 ctgatgccaa cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat
     4801 gtgatcctcg cccaacccgc aacctcagca aaatttgaac gcaaacccat ctactggatg
     4861 ctgagtgaag tcgccaaacg cttaggacca gacgtttatc aaacctttac tgaaggtcgc
     4921 agtcagcatg aatggatcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg
     4981 cccgactacg aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac
     5041 tacgtcgctt tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg
     5101 gggaaaatcg aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa
     5161 aaagatgaaa ttattcatcc cattcctgcg tatacctcag gttttgatgg ctgggacgct
     5221 cccctgcgga aaacctatcc actgcagtta acgggcttcc actacaaagc gcgtacccac
     5281 tccagctacg gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac
     5341 cccattgatg ctcaggcacg cggtatccgt catggcgata ccgtgcgggt atttaacaat
     5401 aatggagaaa tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc
     5461 atcgggcaag gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc
     5521 agtatcaata ttctgacctc tcaccgccct tcaccgctag caaagggaaa cccgtcgcac
     5581 agcaatcttg tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt
     5641 ttttatcgat tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt
     5701 taaagatctt ggcccggaag tcagtttccg ccgtatttat gaatatgctg gcggcgactg
     5761 gcaggaagat aatggcatct ggcaccagaa cgtgttcgct tattatctct ccatctcctg
     5821 taaccattgc gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg
     5881 tgaagatggc tttgtggtgg ttgat
//
LOCUS       DADLTI010000211         5786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLTI010000211 DADLTI010000000
VERSION     DADLTI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.213"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..789)
                     /locus_tag="JN755_003828"
     CDS             complement(<1..789)
                     /locus_tag="JN755_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY7382448.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /locus_tag="JN755_003829"
                     /pseudo
     CDS             1035..1661
                     /locus_tag="JN755_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1658..1864)
                     /locus_tag="JN755_003830"
     CDS             complement(1658..1864)
                     /locus_tag="JN755_003830"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7382449.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1834..2772)
                     /locus_tag="JN755_003831"
     CDS             complement(1834..2772)
                     /locus_tag="JN755_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7382450.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARGEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JN755_003832"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JN755_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7382451.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="JN755_003833"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="JN755_003833"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7382452.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5786)
                     /locus_tag="JN755_003834"
     CDS             complement(4851..>5786)
                     /locus_tag="JN755_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7382453.1"
                     /translation="FISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSEN
                     LMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLII
                     FFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSM
                     YNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
                     VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIG
                     VAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1488 c         1372 g         1444 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
     5341 aagaaaagcc cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc
     5401 acaataccac gattaatcac tctgcgtacc agatagaggc aactggcacc cgatatcact
     5461 gccaccaatg cggcaaagaa aataatcaat gtacaaaccc gcaaataaac atctttttta
     5521 tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg
     5581 gtgaagatgg cgtttttagc attatattca tctccggtca ccggtttacc gttcttaaat
     5641 agagagaaca tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc
     5701 tccagaccaa gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg
     5761 aaggttttac cgtcaaaaga gataaa
//
LOCUS       DADLTI010000212         5768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLTI010000212 DADLTI010000000
VERSION     DADLTI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.214"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..848
                     /locus_tag="JN755_003835"
     CDS             <1..848
                     /locus_tag="JN755_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382454.1"
                     /translation="QEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAER
                     QIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEA
                     DRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQ
                     RALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAA
                     GDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPV
                     AENVE"
     gene            1087..1146
                     /locus_tag="JN755_003836"
     CDS             1087..1146
                     /locus_tag="JN755_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7382455.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1463..1522
                     /gene="ibsE"
                     /locus_tag="JN755_003837"
     CDS             1463..1522
                     /gene="ibsE"
                     /locus_tag="JN755_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7382456.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="JN755_003838"
     CDS             complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="JN755_003838"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7382457.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3123..>5768)
                     /gene="glnE"
                     /locus_tag="JN755_003839"
     CDS             complement(3123..>5768)
                     /gene="glnE"
                     /locus_tag="JN755_003839"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7382458.1"
                     /translation="WQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQTLAL
                     VTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGE
                     LNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMR
                     LRPFGESGPLVLSFAALEDYYQAQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFV
                     FRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGRE
                     PLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDE
                     LNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQ
                     DALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLMPHLLSDV
                     CAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIASQLARYP
                     LLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAA
                     DIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAVVGYG
                     KLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFSTRTSSGI
                     LYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQLTAHFDA
                     VRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFITQYLV
                     LRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPG
                     HVSEDCFTAERDLVRASWQKWLVEE"
BASE COUNT         1368 a         1669 c         1465 g         1266 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagccga tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca
      361 aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg
      421 cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac
      481 aacgtgcgct gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca
      541 aactggcctt gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat
      601 ctattgacgg tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg
      661 atgcaaacac gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc
      721 tctcttaccg cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag
      781 gcggttcact ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg
      841 tagaataaat tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct
      901 gcgttccgct aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg
      961 gaaggcaaaa ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg
     1021 gagtcagcaa agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa
     1081 agggttatga tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct
     1141 tattaacagc caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg
     1201 ttgaaaaaaa atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg
     1261 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt
     1321 tgcgtgaccg ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg
     1381 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg
     1441 ggccttatag aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact
     1501 gttaagtttc ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt
     1561 gttcctttac tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga
     1621 gatctgtgaa ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
     1681 agtggtcgag caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac
     1741 ttctttactc ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg
     1801 caggatcccg gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
     1861 cgcttccagt gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc
     1921 ccctttcagc cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag
     1981 tttgcgggca tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt
     2041 ggtcatcacc actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc
     2101 ttcggtcatc acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat
     2161 cggcgaaacc gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa
     2221 gaagcaggct tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac
     2281 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg
     2341 cgctttaccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
     2401 gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca
     2461 tttaccgaca acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta
     2521 gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag
     2581 ttggatcatc tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc
     2641 gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc
     2701 gaaaccttct tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt
     2761 ggtgatggtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
     2821 agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt
     2881 agcaccgaga gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat
     2941 ggtattcact ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta
     3001 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
     3061 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
     3121 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg
     3181 aagcaatcct ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
     3241 cgcagcgtcg tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
     3301 tgcgccagca gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca
     3361 tgagcgtagc gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca
     3421 tcggctttga tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg
     3481 cgcatttccc gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca
     3541 cgacgcaccg cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc
     3601 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct
     3661 gcggatgtca ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac
     3721 aaaatgccgg aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
     3781 taaaactgcc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca
     3841 tggaggaaga taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
     3901 tagccgacca ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag
     3961 cgggccacca tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc
     4021 caggttaagt gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca
     4081 atgcgtaaca gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca
     4141 tcttccggca cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc
     4201 ggctggtaaa gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc
     4261 tggctggcaa tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg
     4321 aattcactga gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta
     4381 atgcgcgaca gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc
     4441 atcagatggt cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg
     4501 cggaaatcag caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc
     4561 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
     4621 agcgactctt cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc
     4681 acattggtca tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc
     4741 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg
     4801 ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt
     4861 tcagcatcgt tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa
     4921 gagcgcgact gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata
     4981 aattcaattt cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga
     5041 cgcacttcac gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg
     5101 atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
     5161 tcgctatcgc ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc
     5221 gcctggtaat aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac
     5281 ggacgcagtc gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact
     5341 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
     5401 ccctgcgtac aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattc
     5461 agttccccgc cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc
     5521 gcattgcacg gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca
     5581 acaatcagcg tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt
     5641 gccagcgttt gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg
     5701 cgcattaacc cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa
     5761 tgttgcca
//
LOCUS       DADLTI010000213         5755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLTI010000213 DADLTI010000000
VERSION     DADLTI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.215"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..156
                     /locus_tag="JN755_003840"
     CDS             <1..156
                     /locus_tag="JN755_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7382459.1"
                     /translation="ISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     gene            153..416
                     /locus_tag="JN755_003841"
     CDS             153..416
                     /locus_tag="JN755_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7382460.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            409..795
                     /locus_tag="JN755_003842"
     CDS             409..795
                     /locus_tag="JN755_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7382461.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            803..1489
                     /locus_tag="JN755_003843"
     CDS             803..1489
                     /locus_tag="JN755_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7382462.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1467..2090)
                     /gene="tetR(B)"
                     /locus_tag="JN755_003844"
     CDS             complement(1467..2090)
                     /gene="tetR(B)"
                     /locus_tag="JN755_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7382463.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2172..3377
                     /gene="tet(B)"
                     /locus_tag="JN755_003845"
     CDS             2172..3377
                     /gene="tet(B)"
                     /locus_tag="JN755_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7382464.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3490..4083)
                     /gene="tetC"
                     /locus_tag="JN755_003846"
     CDS             complement(3490..4083)
                     /gene="tetC"
                     /locus_tag="JN755_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7382465.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4171..4581
                     /locus_tag="JN755_003847"
                     /pseudo
     CDS             4171..4581
                     /locus_tag="JN755_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4597..>5755)
                     /locus_tag="JN755_003848"
     CDS             complement(4597..>5755)
                     /locus_tag="JN755_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7382466.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1595 a         1069 c         1260 g         1831 t
ORIGIN      
        1 atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg
       61 gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
      121 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac cgaccactat
      181 taagtcatgt agctggtttt atttcaattg aacgttttca aagtctagct acagaaggaa
      241 aaatgttatc gctatcttgg tgggaaaacg aatacgcagt tctgcaatgg aaaaatcatg
      301 ttttacatgc gaaagctcaa caagaagggc gagagtcaat atttgatttt tacaaaatta
      361 gtattgctca tattactcgc gaatattcat ttaaaaagga caaggataat gtttgatgtt
      421 cacgttgttt tagataatca aataggacaa ttagcattac taggaaaaac attaggtaat
      481 aaaggtattg gattggaagg gggagggata tttacggttg gtgatgaatg ccatgctcat
      541 tttcttgttg aacaaggaaa ggaagctaaa atagcgctag agcaagctgg actgttagta
      601 cttgcgatcc ggacaccatt aattcgtaag ttaaaacagg aaaaaccggg ggaacttggc
      661 gaaatagcac gagtattggc ggagaataac attaatattt tagtgcaata cagtgaccat
      721 gctaaccaac tgatattaat aacggacaat gatagtatgg ctgcatctgt tacgctccct
      781 tgggcaataa agtgaacttg cgatggctaa tttaatacga aaagaggtta cctttgagtc
      841 ctcaatagcc gcgatagggg cggctatgtc tgacatttca cgagttaaaa tactcagtgc
      901 tttgatggat gggcgagctt ggacggccac tgagctaagt tctgtggcga atatatcagc
      961 ttcaacggcg agcagtcatt tatctaaatt attagattgc cagctaatca cagtagtagc
     1021 tcaaggcaag catcgttatt ttcggctagc aggaaaagat attgctgaat tgatggaaag
     1081 tatgatgggg atctccttaa accatggcgt acatgccaaa gtttccacgc cagtgcattt
     1141 acgaaaagca cgtacttgct atgatcattt agctggcgaa gttgccgtta agatctatga
     1201 ttccctttgt caacagcaat ggatcactga aaatggttca atgatcacat taagtggtat
     1261 tcaatatttt catgaaatgg gaattgacgt tccttccaaa cattcacgta aaatctgttg
     1321 tgcgtgttta gattggagtg aacgccgttt ccatttaggt gggtacgttg gagccgcatt
     1381 attttcgctt tatgaatcta aagggtggtt aactcgacat cttggttacc gtgaagttac
     1441 catcacggaa aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct
     1501 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     1561 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     1621 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     1681 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     1741 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     1801 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     1861 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     1921 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     1981 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2041 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
     2101 taatttttgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
     2161 agaaaagtga aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca
     2221 tggggattgg ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg
     2281 aagatatcgc taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct
     2341 ttgctccttg gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt
     2401 cattaatagg cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc
     2461 tgtatttagg ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg
     2521 tcattgccga taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa
     2581 gttttgggct tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac
     2641 cgcatagtcc cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt
     2701 tttggttccg tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga
     2761 cgcaatcgaa ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt
     2821 atttttcagc gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa
     2881 atcgttttgg atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac
     2941 actcagtatt ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg
     3001 cagtactgct cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg
     3061 aaggttggtt agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg
     3121 cattacaggg agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat
     3181 tattggtgag ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt
     3241 ataatcattc actaccaatt tgggatggct ggatttggat tattggttta gcgttttact
     3301 gtattattat cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac
     3361 aggagacaag tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac
     3421 cctcttgata acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac
     3481 ctatctattt tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg
     3541 gcctttttta tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca
     3601 gccattgagt aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat
     3661 caatttgctg acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag
     3721 ttcattccaa ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc
     3781 aataatataa tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa
     3841 atcagttttt gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact
     3901 gagtaattgt tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacgcttt
     3961 tcccgagatc tcatctattg aaacagcttg atagcctttt tcaacaaaca atattcgtgc
     4021 tgagttaacc agtgattgat aggtactctt aaaattttct tgttgatgat ttttattttc
     4081 catgatagat ttaaaataac ataccgtcag tatgtttatg gtatcatgat gatgtggtcg
     4141 tgacaatctt aagaacattt aggttatttt atgtatattg aacagcattc tcgctatcaa
     4201 aataaagcta ataacatcca attaagatat gatgataagc agtttcatac aacggttatc
     4261 aaagatgttc tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg
     4321 gcgaataaag cgggttatac caagtggtat tttcagcggc tgttcaaaaa agtaacaggg
     4381 gtcacactgg ctagctatat tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg
     4441 ttgacgaaaa aaactatcct tgagatcgca ttaaaatatc aatttgattc ccaacaatct
     4501 tttacacgtc gatttaagta catttttaag gttacaccaa gttattatcg gcgtaataaa
     4561 ttatgggaat tggaggcaat gcactgagag atcccctcat aatttcccca aagcgtaacc
     4621 atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat
     4681 tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg
     4741 atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc
     4801 cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc
     4861 tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg
     4921 gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg
     4981 aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat
     5041 tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga
     5101 gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata
     5161 gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt
     5221 ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc
     5281 cagcttctca acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg
     5341 tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt
     5401 tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag
     5461 tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac
     5521 aagtacaatg ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc
     5581 gagtcgctct ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg
     5641 ttttgttctc gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca
     5701 gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaat
//
LOCUS       DADLTI010000214         5721 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLTI010000214 DADLTI010000000
VERSION     DADLTI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.216"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="JN755_003849"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="JN755_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7382467.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="JN755_003850"
     CDS             582..1031
                     /locus_tag="JN755_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7382468.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1264)
                     /locus_tag="JN755_003851"
                     /pseudo
     CDS             complement(1032..1264)
                     /locus_tag="JN755_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1350..1523)
                     /locus_tag="JN755_003852"
     CDS             complement(1350..1523)
                     /locus_tag="JN755_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7382469.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="JN755_003853"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="JN755_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7382470.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="JN755_003854"
     CDS             complement(2215..3024)
                     /locus_tag="JN755_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY7382471.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4659)
                     /gene="patD"
                     /locus_tag="JN755_003855"
                     /pseudo
     CDS             complement(3234..4659)
                     /gene="patD"
                     /locus_tag="JN755_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4681..5387)
                     /locus_tag="JN755_003856"
                     /pseudo
     CDS             complement(4681..5387)
                     /locus_tag="JN755_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5377..>5721)
                     /locus_tag="JN755_003857"
     CDS             complement(5377..>5721)
                     /locus_tag="JN755_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7382472.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1497 a         1447 c         1270 g         1507 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gccgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcgtt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggtgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaga catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tgttattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaattcctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaattat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcacgatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcaggcaccc agtttttcca ccagcgtatc
     3781 gtaaatgcct ttttgcgcgt agatccgaca agccgcagta caatcctgtc cagcattgta
     3841 atagccaaat gtacgtacac cttcgaccac tgcttcaata tccgcatcat caaaaacaat
     3901 cactggcgct ttgccaccaa gttccatatg agtacgctta atggacgacg cggtatggct
     3961 gatgatgtgc tcgccggtgg cgatagagcc cgtcagcgac accatccgca ctttgggatg
     4021 accggtcagc ggatcaccca ccgttttgcc tctgccaaac agtatgttaa tcacgcctgc
     4081 cgggaagata tctttcgcca gctctgccaa cttcaacgcg gtcagcgggg taatttctga
     4141 tggtttaagc actacgcagt tccctgccgc cagcgccgga gcaagtttcc acgcggccat
     4201 catcagcgga taattccacg gtgcgataga agccacgccc cccaacggat cgcgacggat
     4261 catcgaagta tgaccttcaa aatattcacc tgccgccaga ccattcagac agcgcgccgc
     4321 acccgcgaaa aagcgaaaaa catcgacaat cgccgggatt tcatcattga acgcactatg
     4381 cagcggtttg ccacaattac gggattccag ttcggcaaaa acctgaccat tttcttcgat
     4441 aacatccgcc agtttcagca ggcattccgc acgcgctttc ggtgtggttt gcccccattc
     4501 agcaaatgct gcatctgccg cacacacagc agcatcgacc tgctctgcgg atgcctcggc
     4561 aatctccagt aaaacgtccc ccgttgccgg attatagaca ggctgttttt ccccttcgcc
     4621 gctaaccagt tctccgttaa tcagtaactt atgttgcata gattattcct gtatcagtgg
     4681 ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc cccaggatcg gcaaggttgt
     4741 taccaacata accagcagtg ccaccacgtt ggttaccggt acatcacgcg gtcgcccaag
     4801 ctgattgagc aaccacaacg gtaacgttcg ttcatgacct gccgtaaagg tcgtaacgat
     4861 gatttcatcg aacgacaagg caaacgccag cattcctcct gccagtaacg ccgaactgag
     4921 attcggcaac actacgtagc ggaaggtttg ccagccattg gccccaagat ccattgacgc
     4981 ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg acattgttaa acaccaccac
     5041 tacgcaaaaa gtcgcatgac cgaccacgat ggtgaaaaat cccggctcca gattgatggt
     5101 tttaaaggcg gttaatagcg ccagaccagt gacaatgccc ggcagcgcaa tgggcagcag
     5161 caataacagc gaaatggcgt ttttgccgaa aaagtctcgt ctccacagcg cggcagctgc
     5221 cagcgtccct aacaccagcg cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct
     5281 tcagtgttaa acgcataggc ggcgattatc aggatgggaa aatgtaggaa aacaacgccg
     5341 ccccaggccg ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag
     5401 acgtttcacg aacgccaggt acagtgcgat gagaataatc ggcaccaggg tgaatgccgc
     5461 cgccatcggc atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata
     5521 tcctggagga ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa
     5581 gatagagcca gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg
     5641 tcgtggacgt gcgccgagat cagccgacgc ctgcaacaat gacggcggca aacgctcaag
     5701 cgccgcctga acgggcagga t
//
LOCUS       DADLTI010000215         5708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLTI010000215 DADLTI010000000
VERSION     DADLTI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.218"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            57..1525
                     /locus_tag="JN755_003858"
                     /pseudo
     CDS             57..1525
                     /locus_tag="JN755_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JN755_003859"
     CDS             complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JN755_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7382473.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1942..2628)
                     /locus_tag="JN755_003860"
     CDS             complement(1942..2628)
                     /locus_tag="JN755_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7382474.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JN755_003861"
     CDS             complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JN755_003861"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7382475.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3600..5645
                     /gene="dcp"
                     /locus_tag="JN755_003862"
     CDS             3600..5645
                     /gene="dcp"
                     /locus_tag="JN755_003862"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7382476.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1427 a         1357 c         1475 g         1449 t
ORIGIN      
        1 tttttattca tataacaaaa catatagctt gccatattta aatttaagga attgttatgg
       61 gaaataattt gttatcagca aaagcgacac tccctgttta tgatcgtaat aacctggccc
      121 caagaattgt tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg
      181 atattcttgc tacagaacat ttcagtgact ggggatatta tgaggtcaac ttaatcggcg
      241 gcgaacagca aattgccgat ttacaacagc aagataatct ttataccgtt gcggaaatgt
      301 cggccgatgc gtggacggct cgcgtcgttg gcgtcgttaa aaaagccttg cacgtacaga
      361 tggatggctt agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc
      421 tgacaatcac cgaaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc
      481 acccgatggt cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acatgggtga
      541 ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc cgcattcccg catttaccgt
      601 catgtcatgt gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta
      661 cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc
      721 aacaatggtg gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca
      781 acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat
      841 cgaagataac tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag
      901 tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct
      961 ggcatatctg ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca
     1021 ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca
     1081 gggcgttgat ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt
     1141 acgtcatcga acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt
     1201 ggattctgtt cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt
     1261 cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag
     1321 tgacccactg ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg
     1381 cgtccagtca ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt
     1441 gtttactgca aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac
     1501 cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac
     1561 ttagttcatg ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac
     1621 aactacggtt tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag
     1681 gatcttgcca gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt
     1741 acggttacga tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc
     1801 acgtaacggg cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag
     1861 ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg
     1921 gatgggatga gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat
     1981 ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc
     2041 gccatcacgt ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg
     2101 tggagaaaca tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc
     2161 aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc
     2221 atcaagttca aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag
     2281 attttgttcc agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac
     2341 cgcgcactca atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt
     2401 gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat
     2461 aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga
     2521 caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat
     2581 ctgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt
     2641 gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt
     2701 acgccgggta taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg
     2761 gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg
     2821 cttcgctgac atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac
     2881 cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga
     2941 tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg cagattcagg ctaaactgac
     3001 gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg
     3061 ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc
     3121 gcgtcatata tactaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc
     3181 tggctttatg cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa
     3241 tattgctcca ctcggcaggg agcgatgcca gcatctcttc aatagcggcg cggttgcgaa
     3301 cgtccagttg ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct
     3361 cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc
     3421 attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg
     3481 cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag
     3541 attgcactcc gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa
     3601 tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg
     3661 atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatgcaa caaaagcggg
     3721 cagaaattgc tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg
     3781 cactggaaca aagcggagaa ttaattaccc gcgttaccag cgtctttttt gcgatgactg
     3841 cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg
     3901 aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc
     3961 agcgccgtga atccctgggg cttgatagtg aatccatccg cctggtggag gttattcatc
     4021 aacgttttgt ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac
     4081 tgaatacaga agctgcgacc ctgaccagcc agtttaacca gcggttactt gcagcaaata
     4141 aatccggcgg tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga
     4201 ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc
     4261 tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa
     4321 aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta
     4381 tcattcaacg tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt
     4441 atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta
     4501 tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg
     4561 cggttatcga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg
     4621 cggaacaggt acggcgggag aaatttgatc ttgatgaggc gcagctcaag ccatattttg
     4681 aattaaacac ggtgttaaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta
     4741 agtttgtcga acgttttgat attccggtct accatcctga cgttcgtgtg tgggaaattt
     4801 ttgatcataa tggcgtggga ctggcgttat tttacggtga tttcttcgcc cgtgattcaa
     4861 aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc
     4921 cggtaattta taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac
     4981 tctgggatga tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg
     5041 cccgccagcg ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc
     5101 cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc
     5161 attatcagag cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc
     5221 tgttcaacaa agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct
     5281 ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc
     5341 tggtggcgga aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg
     5401 cccatatttt tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt
     5461 tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc
     5521 agcgttttcg cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc
     5581 gacaatggcg cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca
     5641 tataaacacc accaatggct ggcctggaga tatcgccagg ccagcctggt tatagcagta
     5701 attgcagc
//
LOCUS       DADLTI010000216         5659 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLTI010000216 DADLTI010000000
VERSION     DADLTI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.219"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(121..540)
                     /locus_tag="JN755_003863"
     CDS             complement(121..540)
                     /locus_tag="JN755_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382477.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILASSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            590..1768
                     /gene="ltrA"
                     /locus_tag="JN755_003864"
     CDS             590..1768
                     /gene="ltrA"
                     /locus_tag="JN755_003864"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7382478.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1851..2030
                     /locus_tag="JN755_003865"
                     /pseudo
     CDS             1851..2030
                     /locus_tag="JN755_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2060..2542)
                     /locus_tag="JN755_003866"
                     /pseudo
     CDS             complement(2060..2542)
                     /locus_tag="JN755_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2798..3778)
                     /locus_tag="JN755_003867"
     CDS             complement(2798..3778)
                     /locus_tag="JN755_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7382479.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3778..4977)
                     /locus_tag="JN755_003868"
     CDS             complement(3778..4977)
                     /locus_tag="JN755_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7382480.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5299..>5659)
                     /locus_tag="JN755_003869"
     CDS             complement(5299..>5659)
                     /locus_tag="JN755_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7382481.1"
                     /translation="TFHPSLTQHWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTP
                     FCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIERNNRTLRTRIKRLARKTICFSRSV
                     EIHEKVIGSFIEKHMFY"
BASE COUNT         1559 a         1285 c         1346 g         1469 t
ORIGIN      
        1 taaaacaagg actgcccggc tgtgggtcta cgttcgtgat gaccgtaacg ccggttcgga
       61 aattgcagca taagttatcc atgctctgag gtgggtggag gtgggccctc cggtcagggt
      121 ttacaggagc actgaccaaa cgacccggtg ctgcttcatt ctaaggaatg aggtggtgag
      181 cgactcggga aaaggcagag atctactgtc acgtggggtt cgggaagctg aacgaaagta
      241 aactgtccga tgaagcatcg aaatcttaga tgatgccaaa atggggccgt acagccgtcc
      301 cctgataagc gcagaagaca cctgcttact gactgcgcgg catccggtgt gaaggcagca
      361 ggatcccggg ccagaccatc tcagggaacg tgagaacctg tttccggcac gtatcgggaa
      421 acaggggcgg acccattcgt agtagtgatg aaagatctgt aatgggtctg gagcgaaggg
      481 atggggatca cattcattgc agaagacaca acccaaaagg ggatgacgta actgatgcaa
      541 cgaaaatcat ttgaaatacc aaaagcactg gtctgggcat catatctgga tgtgcgtcgc
      601 aacaagggag ccccccggat gcgacgggca gactttgaaa atgttcgacc agcaacgtga
      661 tggcaatcta tacaagatct ggaaccggct atgttcggga acatggtttc cgccaccagt
      721 gctggaaaaa cggatcccga agtcgaatgg caaggaacgc attctgggga tcccaacagt
      781 atccgatcga attgctcagg gagcgataaa acttttcatg gaagaaaagc ttgacccgat
      841 tttccatgct gattcatatg gttaccgacc aggcaaatca gctcatgacg ctctgaaaca
      901 atgtgccatc cggtgctggc gttatagctg gatactggaa gtggacatca gtgcgttttt
      961 cgaccatgtg agacatgatc tggtgctcaa agctctggaa caccacggga tgcctaaatg
     1021 ggtcatcctg tactgtcgac gctggatgga agcgccaatg cagagttgtg aaaatggaga
     1081 attaataacc cgaacacgag gcactccgca gggcggggtc attagtccgc tacttgctaa
     1141 cttgttcctt cactatgcat ttgatttgtg gatggaaaga gaatatcggg gggtaccgtt
     1201 tgagaggtac gctgacgata ttgtagtaca ttgttcacga atgagtgatg taacaaggct
     1261 aaagaacagg ttatcggagc gcttctcgga agtcgggctg gtcctgaatg cagggaaaac
     1321 gaatatcgcc tacattgaca cgtttaaaag gagaaacgtc gcaacgagtt tcacctttct
     1381 cggatatgac ttcaaagtgc gtacgctgaa gaatttcaaa ggcgaacggt accgaaaatg
     1441 catgccgggt gcgtcaaatg cagcaatgcg caaaataaca gaaacaatca agaagtggcg
     1501 tatacatcgc tcaacagctg agagtttgct ggattttgcg aggcgctaca atgcgatagt
     1561 gagaggctgg atcgagtact acggaaagtt ctggtccaga aatttcaact atcgactgtg
     1621 gagtgcaatg cagtcacgtc tgctcaagtg gatgcagtct aaatacagac tttcgaaccg
     1681 gaaggctcag cgaaagctga cgctggtaag gaaggagtat ccgaagctat ttgttcactg
     1741 gtatttacta cgtgcatcaa atgagtgatc aagagccgta tgacgcgaga gtgtcacgta
     1801 cggttccgtg agcagccggg gagtgaaatt ctctccggct gactcgactc gcgggttccg
     1861 acagcgatga cgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca
     1921 atgtggaacc agaaaaatgg ctgcgttacg tcattgagca tatccaggac tggccggcaa
     1981 accgggtacg tgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaatac
     2041 ggttctgacg agccgcttac ttttggcgta attctgctgc gcctttactg aacacaatgc
     2101 cgtattcagc aagaagtcca cgaatgtgat ttatcagttg tgtcctttcc attgaaacca
     2161 accgacgcat attcaaaaaa gtctggacgt cctgttgctc cagagttttt attggtacgg
     2221 gtcgtatagt tccatgttgg caggcctcag ctattgcctg tgcgtcgtta taatcatttt
     2281 tctggcaccg ctgataagct ttaacatgtt gtgccggaag caatattaca tcatggtcga
     2341 gtttcctgat ttcccgggcc cagtaatgag atgccccgcc agcctccatc gcaacagtca
     2401 ttttaggctg ttgcacctgc cagttaagca gttgattgcg agaaagtttg gtctttcctg
     2461 cgggtttgcc atgagcattg atactgaaaa gataaaagtt cgttttagcc agatcaatgc
     2521 cggtgatttt ctgttcagac atggtatccc gcttactttt gtggttaaag tttatccatc
     2581 atggcgcaat tgaaggctgc cgtgaaagag ggggaagact atctcatcgg ttactgttaa
     2641 acggctaaga gaaactatct ttttcaagca aagaagatac ggtgaaacag gcgatttcac
     2701 cgtgaaatac tgttcaaaaa aaacgctaat aaaagcaaaa tttcttttaa aacagccaat
     2761 taaaaactgg tgagttgctg gcgaaaaact tgaattttca cagcgaattc agattatctt
     2821 ttagtacaag agcaatcata cgatctaact tatcctgaac ttccaacggt aatctgccga
     2881 attcatatga gaacgtacgt ccctttactc gttttcgagc aaatttatct ttactatcaa
     2941 atttccataa caaagttaca accgctttat cctttactcc tgaatcaagc aatgaagaca
     3001 tttccttggt tataagccgc ataatactgt ttttaacctc gtcttcagaa agattgtcat
     3061 cagcagtaat aagctcaata ctcggcgaga tattcttcac aacttcatca atagatatat
     3121 cacccagcct cagtcgttca cttaattcag ccagttgcct gtaatcggcg tatgtcagct
     3181 cagactgaac aggaaaaaga gacacaaaat ctttaggtac ggacgctgct tggagggcac
     3241 gagtcacctt agctgccgac aatccctctc tttccgcaat ttgcttctgg ttcataccgg
     3301 cctctttgag acgtactaaa cgcaacccaa tttcacgaat attatgttca agtgacgtct
     3361 gcaaatcttt cgccagatgc tgagcttcag aaacagtaag ctcatcatcc gttacaagta
     3421 cacgtaatcc aaccttgcat agcaaagctg ctgcccgacg acgggaacca tccaaaatct
     3481 caataagatc gccagtccga atcccaatac atggatagaa ttgttgtaaa cgtatagttc
     3541 tggtgatgtc ttttagtgag tccggcgtca gggctgtctg ttcgcggcca ttcacctctt
     3601 caaccacaaa agtttgagtg tccactttgt catgatcaac cttgatctca gtaaattttg
     3661 cctttcggcc agtattgagg gtaaatacat gaactggagc cgatatttct tcagtattat
     3721 tcaggatatt tgtattcagc gtacggccaa tagtcggtcg gtgctttctg ttttccatca
     3781 gtagttggcc cttataaatt caatacgatc aaaaactgct ttagcgaaat cttctgctgc
     3841 catacgtgca tttttcaaag cttcaccact gccaacataa gtaacaggat tcgcagatat
     3901 cacggtatca aacgactcgc cgcttctctc aaaaccatca agacgtggca ttgaaacatc
     3961 aagcatgtca ccaccgaaaa tctcttttgt caggctatgg cagtatttat gatctgactt
     4021 attcgccagt ttcgacatga agccaatatt tgcctgcaaa cggcatgaac atccatcctg
     4081 ttcaataatc tgtacaagtt caggtaaacg agccaaatat ttaagcgtgg aatggaaatc
     4141 aacttgtgct ggcggcaccg gtgtaaacat gatatctgcg gcagcaatgg catttttcaa
     4201 aaatgcgtcc agatgcgggc ctgtatcaat aaggatgaag tcgaaatcat gcttcagctt
     4261 atcaataata ttctcacgca atatcgcgtg tttgttttgt cctaatagat gctcctcgca
     4321 cagagtatcc cagtttgatg caataaaggc gtcgtcaata gaagccggca taacataaac
     4381 gccagggata actgacggaa cgataaaatc ttccagtaat tcctcacgac tcacgttctg
     4441 caacatagcc tgcggtgcgg ttgtatcaac cagccccacc gcatgcaagt agttcagaaa
     4501 catagtggct gatgactggg gatctaaatc aaggagcaga atacgtaaat cttctgacag
     4561 caggtgagga tgagcacgca gggcgtgagc tacggaaaca ctactcacgg ttttagacac
     4621 accgcctttt agactaccga tgaatatgga atatgcctca ctataacgat cgcgatattt
     4681 aggaatgcca cgatgtgcat agatatcgat aatgttctgg atggttaatg catattttgt
     4741 cgcggttcct gctgggcgtt tttcaaactg atagccctga gcctccatct caccgactgc
     4801 accatccacg cttgcgcgcg ttaattttgg aagcttagct acagcagact ttgaatagac
     4861 ttgataaaaa acatcagcct gaaactcctg cttttgctcc tgaatctgtt ttgttagggc
     4921 gagcaacatt ttatctgcac gctgcgcaac cttacttaac tgctcaaaac tagtcattcc
     4981 ccctccgtgc tatgtaggat tttaacttat tacaaaaaaa actgcattag cagggtaatg
     5041 catgcaagta catattgtac atgcttatat taaaaatgcg cgcattgctg atttattaat
     5101 catcggaatg aatattactg taagatagta tgcctttccg ggagaaagac gctgatttcc
     5161 cccgaataat ccagttcacc accacactcc ggacaacagc tttcttcagg ctccagatgg
     5221 tgtatttcgc gggaaacatg ttccggtaac ggacggcgat gacgtgactg tcgcaacagg
     5281 cgggggtagt gcttccaatc agtagaacat gtgcttttca atgaaggagc cgatgacttt
     5341 ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg
     5401 ggtgcggagg gtccggttgt tacgttcaat acgttgcgtg aagattttgc cggtcaggtg
     5461 tttctccttc ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat
     5521 acagaacgga gtcaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa
     5581 agtgtaagcc aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgtgt
     5641 caacgacgga tgaaaagtg
//
LOCUS       DADLTI010000217         5599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLTI010000217 DADLTI010000000
VERSION     DADLTI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.220"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..410
                     /locus_tag="JN755_003870"
     CDS             <1..410
                     /locus_tag="JN755_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7382482.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JN755_003871"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JN755_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JN755_003872"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JN755_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7382483.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1401 c         1330 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DADLTI010000218         5553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLTI010000218 DADLTI010000000
VERSION     DADLTI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.221"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..638
                     /locus_tag="JN755_003873"
     CDS             <1..638
                     /locus_tag="JN755_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382484.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JN755_003874"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JN755_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY7382485.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="JN755_003875"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="JN755_003875"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7382486.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1357 c         1540 g         1280 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac atcggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc tta
//
LOCUS       DADLTI010000219         5537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLTI010000219 DADLTI010000000
VERSION     DADLTI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.222"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN755_003876"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN755_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7382487.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN755_003877"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN755_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7382488.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /locus_tag="JN755_003878"
     CDS             complement(2659..>5537)
                     /locus_tag="JN755_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7382489.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1462 a         1288 c         1303 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DADLTI010000220         5534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLTI010000220 DADLTI010000000
VERSION     DADLTI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.223"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            103..1011
                     /locus_tag="JN755_003879"
     CDS             103..1011
                     /locus_tag="JN755_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7382490.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="JN755_003880"
     CDS             complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="JN755_003880"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7382491.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3326..4084
                     /locus_tag="JN755_003881"
     CDS             3326..4084
                     /locus_tag="JN755_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7382492.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4290..5210)
                     /gene="speB"
                     /locus_tag="JN755_003882"
     CDS             complement(4290..5210)
                     /gene="speB"
                     /locus_tag="JN755_003882"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7382493.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5346..>5534)
                     /locus_tag="JN755_003883"
     CDS             complement(5346..>5534)
                     /locus_tag="JN755_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382494.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1325 a         1483 c         1478 g         1248 t
ORIGIN      
        1 tttttcgcta taccccttta tagattctga attctcacct atcggctgtt ttctcctcat
       61 ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc acctgtttca
      121 ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct tgccgtggtt
      181 gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc
      241 tacggaaaaa tcgtaaaagt tgccgagccg gggctgggtt ttaagatccc ttttatggaa
      301 agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt acaggcctac
      361 agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttccacat taaaccttca
      421 gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga tcgtctaatt
      481 gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc catcagcgcc
      541 gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa agccgttgtt
      601 gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga cgcttatgaa
      661 aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa gcaaaatctg
      721 gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga agcagacagt
      781 aaactggcgg cagacaaagc agaagcggaa actattcgcg taagaggcgc ggcagaggct
      841 gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg cggtgaagct
      901 ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga cggtaaactg
      961 cccgaaacca tgatccccgg cagcacagtt ccgtttatct ctactaagta aaaaaagggc
     1021 cgctttcgcg gcccttctca caaaacgtct tactgattac agcagcgctt ttgctttcgc
     1081 aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg gagcagattc
     1141 accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt accagtagtc
     1201 agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta cggattcacg
     1261 gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca cgcgcgcttt
     1321 cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt cagaaccggt
     1381 ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac caccgcgcgc
     1441 gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac gggagaggat
     1501 aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg cggattcaac
     1561 ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag cgacctgctc
     1621 taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat
     1681 cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt attccacgaa
     1741 catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac cgttagcaat
     1801 cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag catcttcgtt
     1861 gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag cagagccgcc
     1921 gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg ctttacggct
     1981 ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt tggcgtcgaa
     2041 gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct gcggataagc
     2101 tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc ctgcttcttt
     2161 cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt tccagcccag
     2221 ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg agtcgtgagt
     2281 accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca gggacggttt
     2341 gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt catgaccgtc
     2401 gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt cgtcggtgaa
     2461 ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg caatcagttt
     2521 acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt ccatcatgca
     2581 gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg gacggttaaa
     2641 ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa taccctgacc
     2701 cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccgggt ggcccggagt
     2761 tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt aaccggtgag
     2821 gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga agcggtcacg
     2881 gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc
     2941 aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct gtactgcgtc
     3001 catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt tgactccaga
     3061 tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg gctacgccgg
     3121 aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat ccttttgccg
     3181 cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc
     3241 ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt ttagaataat
     3301 cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag caatgagcgt
     3361 ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct catcaggagc
     3421 ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc
     3481 atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat acgctaaacg
     3541 tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg tgaattacaa
     3601 agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta tccgcgtcta
     3661 tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg gtcacgagat
     3721 ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg gtacaaatgc
     3781 cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc aatcacaact
     3841 tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg aagcagaagc
     3901 cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag gtcttgccac
     3961 cagctttgaa aaactggcga agctggaaga aggccgtcaa agctcaatgt ttgacgacca
     4021 tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg atgggattaa
     4081 gtaaagcctg gtggtgtcga acgtaggtca gataaggcgt tcacgccgca tctgacatta
     4141 atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat cttatccggc
     4201 ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca ttaatggcac
     4261 gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc ctgaatatac
     4321 agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc
     4381 ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc gcgtaccagt
     4441 ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc cggcgcgaaa
     4501 gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc cacaatctgt
     4561 ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg
     4621 gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga atgattcggg
     4681 tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa ttcacaaccg
     4741 ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc
     4801 gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga gagcatacgc
     4861 ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat ctcacgggca
     4921 tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc acgcatgtcg
     4981 aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg acggatcgct
     5041 gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg cacgccagta
     5101 atcacccagt ctgcatcgct gtcatacggc tggaagttca tcggcaggcg taaaaaacca
     5161 aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat ggatggactc
     5221 ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt
     5281 ggatttgcga aacacaagtt tgtgctaatt atcgccttaa atgggcgagg catcaagcga
     5341 ggttgctatt tccgacacgg tggcggcgca taaccattgt tatctgccgg aaaggcttgt
     5401 ccagagcacg gcggcagcgc gttttgcgcc gggccttgta attcaaaggc ggcaaagccg
     5461 attaccccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga atcagcaacg
     5521 ttggcgaact gaaa
//
LOCUS       DADLTI010000221         5498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLTI010000221 DADLTI010000000
VERSION     DADLTI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.224"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            60..797
                     /locus_tag="JN755_003884"
     CDS             60..797
                     /locus_tag="JN755_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7382495.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1131..3410
                     /gene="ydeP"
                     /locus_tag="JN755_003885"
     CDS             1131..3410
                     /gene="ydeP"
                     /locus_tag="JN755_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7382496.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3658..3855
                     /gene="safA"
                     /locus_tag="JN755_003886"
     CDS             3658..3855
                     /gene="safA"
                     /locus_tag="JN755_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7382497.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3929..4690
                     /gene="ydeO"
                     /locus_tag="JN755_003887"
     CDS             3929..4690
                     /gene="ydeO"
                     /locus_tag="JN755_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7382498.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5092..>5498
                     /locus_tag="JN755_003888"
     CDS             5092..>5498
                     /locus_tag="JN755_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7382499.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1587 a         1178 c         1213 g         1520 t
ORIGIN      
        1 cttaaaatca ccatcataca ctaaatcagt aagttggcag cattaccctg gttgtagact
       61 tgtctcagca tataagttgc tggaatgatt acggcggctg gtacgacact gatcatataa
      121 acctggtaca aggctcagct ttcgcaggct ccctacagtc atataaagga tcgctgtact
      181 ggaataatgt gacctacccc ttcccgttga cgacaaatac caacgtattg gatattggtg
      241 ataagacacc gatgccatta ccgttaaaac tctatattac cccagttggg gcggccggtg
      301 gagttgtcat aaaagcgggg gaagtcattg cccgtattca tatgtataaa attgcaacat
      361 taggaagcgg aaatccgcgt aattttacgt gtaatattat ttcaaataac agtgttgtta
      421 tgccaacagg cggctgcacc gttgatagtc gtaatgtcac cgtggatctc ccggactttc
      481 ctggaagcgc agaaataccc cttggcgtat attgctcaag cgaacaaaag ctctcatttt
      541 atctttcggg tgccaccact gacagtagcc gacaagtgtt tgctaacaca gcacctgatg
      601 caacaaaagc atcgggagtg ggtgtcactc taatgcgtaa tggaaaaata ctggctacgg
      661 gagaaaatgt ctccctgggg acagtaaata aaagcaaagt acctctgggt ttaagcgcaa
      721 cctatggaca aacaggaaac aaggtttcag ccggaaccgt ccagtcagtc attggtgtca
      781 cttttatcta tgaataattg tgattttgac cttagtctat gcgctccatt cacgaaaatt
      841 gcgcggataa ttattttgtg aaggctatta tcctacacct gtaagaaaat ccgcgcacaa
      901 ttgaagccag agtcttattt tttatattga aaaatatgtt aatttaaaca ctatgagaga
      961 tgatgcttaa cttatcgtat ttaatctatt gtttaacgat aatagattaa aaatagaaag
     1021 agagtagtcc cctactctta tatatccatg ttggcgataa tccctttgta tgtactgtgc
     1081 atcatcgcta ttacaaatcc taataattca tttccacaca ggataagtag atgaagaaaa
     1141 aaattgaatc ctaccagggt gctgcaggtg gttggggtgc tgttaaatcc gtagcgaatg
     1201 cagtacgtaa gcagatggat atacgccatg atgttattgc catgtttgac atgaataagc
     1261 cagagggctt tgactgtccg ggttgtgcat ggccagatcc taagcacagt gcgtcattcg
     1321 acatttgtga aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg
     1381 cctctttctt tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg
     1441 aggctgcggg gcgtctcact cagcctttga aatatgatgc cgtcagcgac tgttacaagc
     1501 cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg ccttcaaagc tatagtgatc
     1561 ccaatcaggt tgaattctat acttcgggcc gcacttccaa tgaagctgcc tttctttatc
     1621 agctttttgc ccgtgaatac gggagcaata actttcccga ctgctccaac atgtgccatg
     1681 aaccgacaag cgtgggtttg gcagcgagta tcggtgtagg taaagggacc gtgttgctgg
     1741 aagactttga gaagtgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc
     1801 ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgccatca
     1861 atcctctaca ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc
     1921 tgacgaactc tgagactcag ttggccagtg cctactacaa cgtgcgcatt ggtggcgata
     1981 tggcgttgct caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagtgccg
     2041 caggtcggcc ctcattgctt gatgacgaat ttattcaaac gcataccgtc ggctttgacg
     2101 agctacgccg tgacgttctc aattccgagt ggaaagatat cgaacgtatt tctggactaa
     2161 gtcagacaca aatcgccgaa ctggctgacg catatgccgc tgccgaacgc accattatct
     2221 gttacggaat ggggatcact cagcacgaac atggtaccca gaacgtgcag caactggtca
     2281 atttgctgtt gatgaaaggt aacattggca agcctggtgc gggtatctgc ccactacgtg
     2341 gtcactctaa tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt
     2401 ttctggctcg cctgggtgag cgctatggct tccccccacc tcatgcacct ggacatgctg
     2461 caattgccag catgcaagca atatgtacgg ggcaggctcg agcattgatc tgcatggggg
     2521 gcaactttgc actggcaatg ccagatcggg aagcgagcgc tgtaccgtta acgcaattag
     2581 atttggcggt acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata
     2641 gctatattct gccggttctg gggcgtagcg agattgatat gcaaaaaagc ggtgcgcagg
     2701 cggtaaccgt tgaggattca atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg
     2761 ccggtgtaat gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag gcagcactac
     2821 cccagagcgt ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca
     2881 ttgaagctgt gctgccagag ttcgccgact ataaccagcg catccgtcat cccggtggtt
     2941 ttcacctgat aaatgcagct gctgaaaggc gctggatgac gccgtcaggt aaggctaatt
     3001 tcattaccag caaagggctg ttagaagatc cctcttcagc gtttaacagt aagctggtca
     3061 tggcgacagt acgcagccac gatcagtaca acacgacgat ttatggtatg gatgatcgct
     3121 atcgaggggt attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt
     3181 gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct tacgccagac ggtaagcgca
     3241 gctcacgccg catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg
     3301 tgacctattt tccagaatcg aatcacatgc taacacttga taaccacgat ccattaagtg
     3361 gcattcctgg ctataaaagt attccggttg aattagaacc atcaaattaa tgcctcttct
     3421 catttcttct gctgtcatcc gcacagcaga agaattcctc attgactatt attgcgcaat
     3481 ttactcacat ggattaaatt aaactacata ctataagata taaacttctg cctacagctg
     3541 taagaaactc cgctcagtac tgaagcacca gtcctatttc ttcttttctc cagcctgtta
     3601 tattaagcat actgattaac gacttttaac gttatccgct aaataaacat atttgaaatg
     3661 catgcgacca cagtgaaaaa caaaatcacg caaagagata actataaaga aatcatgtct
     3721 gcaattgtgg ttgtcttatt actgacactt acgttgatag ccattttttc ggcaattgat
     3781 cagctgagta tttcagaaat gggtcgcatt gcaagagatc ttacacattt cattatcaat
     3841 agtttgcaag actgaaaata aacagcaaat tataaatgtg aaatgtttaa aaggtatcga
     3901 taaaacttta ttgttttaag gagataaaat gtcgctcgtt tgttctgtta tatttattca
     3961 tcatgctttc aacgctaaca ttttagataa agattacgcc ttctctgacg gcgagatcct
     4021 gatggtagat aacgctgttc gtacacattt tgaaccttat gagcggcatt ttaaagaaat
     4081 cggatttaat gaaaatacca ttaaaaaata tctacaatgc actaacatcc agacagtgac
     4141 ggtgcctgtt cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat tgcttaatga
     4201 aatgattgct tatctcaact cggaagaacg caatcatcat aatttttcag aacttttgct
     4261 tttttcttgc ctgtctattt ttgccgcatg caaaggtttc attacactat taactaacgg
     4321 tgtgctatcc gtttccggga aagtgagaaa tattgtcaac atgaagctgg cgcacccatg
     4381 gaagctgaaa gatatttgtg actgcctgta catcagtgaa agcctgttga agaaaaaact
     4441 aaagcaagag caaacgacat tctcacagat tctattagat gccagaatgc agcacgcaaa
     4501 aaatttgata cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg gttatgccag
     4561 tacatcttat tttatttatg cgttccgcaa acatttcggc aacagtccga agagagtttc
     4621 taaggagtac cgttgtcaaa ggcacacggg tatgaatacg ggcaacacga tgagtgcttt
     4681 agctatttga ttatttgcta acgagtagtc aaccacgcac gctacgtaag aattatatgg
     4741 ggcagccatt ccctgccccg cgttgttttt aggcgatata tttattgaaa taaataagtg
     4801 acatccatca catatttatg cacttgcata acctgttgca tgattattta taatctcaat
     4861 tcggcatttt gtcagtaaaa tgcaataatt tattaaatat caataaatta gttatttatc
     4921 ggcgagaaat tacttaatag aacagaaagt aatgtcaacg ctttatggac tgttatttcc
     4981 ctttttttag ctaaatctgc tatctcttta tgtgactaac ttcacttaca tccacttatt
     5041 tctcttcgta aaattacttc ggaattaagt acaataagaa gaggaacatt tatgaagtct
     5101 gcattaaaga aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct
     5161 gcatttgctg ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct
     5221 ttctcagact ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtacta
     5281 accatggatg atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa
     5341 acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa
     5401 gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact
     5461 aacggctatg tggcacacgg tgtttccggc ccctcccg
//
LOCUS       DADLTI010000222         5465 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLTI010000222 DADLTI010000000
VERSION     DADLTI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.225"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(296..580)
                     /locus_tag="JN755_003889"
     CDS             complement(296..580)
                     /locus_tag="JN755_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY7382500.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="JN755_003890"
                     /pseudo
     CDS             614..1420
                     /locus_tag="JN755_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="JN755_003891"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="JN755_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="JN755_003892"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="JN755_003892"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7382501.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="JN755_003893"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="JN755_003893"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7382502.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="JN755_003894"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="JN755_003894"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7382503.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="JN755_003895"
     CDS             complement(4472..5386)
                     /locus_tag="JN755_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382504.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1380 a         1305 c         1457 g         1323 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacc
//
LOCUS       DADLTI010000223         5444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLTI010000223 DADLTI010000000
VERSION     DADLTI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.226"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..87)
                     /locus_tag="JN755_003896"
     CDS             complement(<1..87)
                     /locus_tag="JN755_003896"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7382505.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JN755_003897"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JN755_003897"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7382506.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JN755_003898"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JN755_003898"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7382507.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1691 a         1227 c          902 g         1624 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact ggaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gaggggaaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aact
//
LOCUS       DADLTI010000224         5411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLTI010000224 DADLTI010000000
VERSION     DADLTI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.227"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(143..376)
                     /locus_tag="JN755_003899"
     CDS             complement(143..376)
                     /locus_tag="JN755_003899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382508.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(373..1644)
                     /locus_tag="JN755_003900"
     CDS             complement(373..1644)
                     /locus_tag="JN755_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7382509.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2038..3408
                     /locus_tag="JN755_003901"
     CDS             2038..3408
                     /locus_tag="JN755_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382510.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3590..5411
                     /locus_tag="JN755_003902"
                     /pseudo
     CDS             3590..5411
                     /locus_tag="JN755_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1671 a         1128 c         1044 g         1568 t
ORIGIN      
        1 cagtgtattt ataacatcgg gagcagattt accaccgtat atgctgtcat acaattcatg
       61 cgctttctta cttgccacca cgcctgatga aactatcagc attaaagaaa ataatattct
      121 attaggcaac ataaaatatc tcttatttat cttgtacctg aatatgctct tcttttaata
      181 aatcacgttg ttttccattc agtatcgctg tcgcagattt aatagaataa gtcaaatcat
      241 gaaaatcgca taaattacga ttatacattg ggatgtctat atagcttttt cttatataac
      301 tggaattatt atacatatcc acaccgagcg tattcaaact aaaaggaggg acagaaagag
      361 tatattcttt actcactccc tccccccccc ttttagctca agcgtcaact gcaaattagt
      421 tatactacta attttatctg cattaatttc aatactgaca tcgcatattt tgggtttttc
      481 atttgcgtta aagccatagg tcccgtaatg ttttaacgtg atgtaaggta aagtatcacc
      541 ctgtgtttct cgggttttct gatcttgatt acaacgggtt aaatattcat ccttattgaa
      601 tttcattgaa tatttccccg cacctttttc gtaataatcc cataccttat cacaatcaag
      661 cgtttcacct aatccgttgt aatacatcca acttaaccac aatgcggctt tttttgcccc
      721 ttgctgttca gccttgtcaa aaagcatgct cgctttagaa taatcaacct caccggtcaa
      781 accagaatag tatatattcc ccagggccac taatgctttt tcgtcattct tatttgcagc
      841 taattcgtac cattttttgg cttcgacagc atcaatcttt aaaccagcac tgccattttc
      901 gtaggtatcg cccatggtat actgcgccat cggataacct ttttctgccg cagtacgaaa
      961 ccattgcagt gctttcttta tggtgtctaa agaacgtcta tcatcagaag gtgaaaaatc
     1021 atcgagataa agatttccga caatataatc catttcgggg tcgccttcct gcgcccatag
     1081 agtcgccaga cgcagaattt caggtttact gaaaccaatt cgcccccaaa aaagcaaatc
     1141 attctcgggt gcctgaccga cataacgact gacaaattca attaaactcg cccgtttcgt
     1201 tttgtctgtc tcagccccgg atttcaattc ataggcctga ttgattattt tattcatcaa
     1261 atcaggcgga atattaagat cgtcagtttt cgacttactg gcatacttct gtgctcgtgt
     1321 cacatctagg gtgacgcctt taccatattg aaagaaataa cgtgcattat aacaaccgta
     1381 ttcgcccccc tggttacagg ctttctcata ccactgaatc gcctttggga tgtcaacaga
     1441 tactgtcact ccgtgttcat aaataaatcc cagtagcact agcgcctgcg tgttattggt
     1501 atcagctaat tttttcagtt cttttaacgc cgtctgatct ttattgacaa tacgttcata
     1561 cagttgatca atatctgttg aagcatgaca aaaagaagcc agagataaac ccaatgataa
     1621 taaaataact ttaccagacc acatacattc ctccttgata tattcaacca ccaaacatcc
     1681 acctcatgaa taaatacgct gccataacgg cccaggtaat aagtaaacat ttaagctgaa
     1741 acagcataac cagtgcctcc aacgcttcta tttattgcga taaaataaca ccatcctttg
     1801 ataaaaagtc acacgcccat caataaatcc aaaaataatg tcattagttg caggataaaa
     1861 tcatttgagc ttttcatttg ttatatttaa cgcatgatgt attaatggca agaatatgag
     1921 agaaactcat acctgacgta aaaatcactc tcttatgtaa tatttactta ttaacactct
     1981 gtttacctaa catgcgctcc gcactacact atgtgggcaa ttttttaaga ggtggtaatg
     2041 aacgattata aaatgacgcc aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt
     2101 tcattgcgca tgctgggcat gtttattgta ctgccggttc tgactacgta cggtatggca
     2161 ctgcaaggcg ccagcgaagc attaatcggt attgccattg gtatttatgg tctgacccag
     2221 gccgtttttc agattccgtt tggcctgctt tccgaccgca ttggtcgaaa accattaatt
     2281 gtcggtgggc tggcagtgtt tgccgccggt agcgttatcg ctgcgctttc tgactccatc
     2341 tggggaatta ttctgggccg ggcgctacaa ggctccggtg cgattgccgc tgccgttatg
     2401 gcgctgcttt ccgatctcac gcgcgaacaa aaccgtacca aagcaatggc gtttatcggc
     2461 gtgagctttg gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa
     2521 cttgggctgc acgcgctgtt ctggatgatc gctattctgg caacgaccgg cattgcgttg
     2581 accatttggg ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg
     2641 aaaggcagtt tcagtaaagt gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt
     2701 atgtgtctgc atattttgct gatgtcgacg tttgttgccc tgcccggaca actggctgat
     2761 gcggggttcc cggcggctga acactggaag gtctatctgg cgacaatgct aatcgccttt
     2821 ggctcggtcg tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt
     2881 gtcttctgcg tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc gcaaacgcag
     2941 ttctggcaac tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc
     3001 ctgctgccct cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt
     3061 gtttactcca ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt
     3121 gacggcatgt ttgacggtca gcgggtattt ctcgctggcg caatgctggc cgcagtgtgg
     3181 ctggcagtcg ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc
     3241 ccagaaaacg taatggcaga tgaaaaactg gaaaaatgct tactgacaac gcctggtgtc
     3301 aaagatgtac tgctggcgaa gttagaacat tgcgtctatg tgaaagtaga cagtaaaatc
     3361 accaatcgtt ttgaaataga gcgagtaatc aacgaagtat cttattaaat aataagcacg
     3421 gtgctattca tgaaaaataa aatagcaccg tacttcgtaa caccataatc ctttaaaccc
     3481 tccattatta atacttcgct taattccata taactcgacg attacattag aagtaaacta
     3541 tactttttat gaaatatcag ggataaatta ttcacatgga aaatacatca tgtacaaatc
     3601 attatttact attatcattc tactgtatag cgctagttca tatgcaaaaa gcgaatcccc
     3661 tcccgcaagc actataactt tcattgcaca aatcgatcat gttgagctaa ataaaacagc
     3721 attatctcat gatatgaaac tcgttcttgc cgatcgtttt tatttcaaaa ccagtaatcc
     3781 ttgcgaaaca aattcaattt ctgctcgacc agaatcaatg gatattactg acaaagccta
     3841 tgccaacaaa ttaaaaaaat tagaaactcc ttttcttgat gatcgctatt tctttatgac
     3901 catcaatcag tgtgacggta atggcactcc catgctgaca gacataaaag tatgtacaac
     3961 agaattatgt ggtgctgaat atacaaagaa aatgtctact gtatggctcg atgaaacttt
     4021 aaaaccaact gctaaacgtc tggcaattta ttttattcaa tctcctttac cctatgacga
     4081 ggagaaaaaa caatggaaag tcaccggatg gtatatctca tcaactgata atcagtcttc
     4141 aactaaacag attgcatttg aaggatatac agacacagat aacttctccg attctaaatt
     4201 tatttcttca tttaaatcct ggtttgaaag tggaaagata aaaaacattg ctaactataa
     4261 tccaaaagga gaaagagatg gaaaatacga aagttattac aatagcaatg gtaacgtcta
     4321 tgaatctcta agtttttcta atggtcttat caacggtgag tttattacat attatgaaaa
     4381 tggggccatt gaaagtaaac gtcattttaa taacgacaaa attaaagatg gtgaatgccc
     4441 tcatttttat gaagatggga aaatcaaaca gcaacatagc tatctgaata gtaaactcga
     4501 tggtcctgca tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca
     4561 aagtgtgttg attggaaacg ataccagtta ctaccaatcc ggaaaaataa tctctataca
     4621 caatagaaat tcccgtggtc agtatgatgg agctaacgaa cgttatagtg aagagggtaa
     4681 gttacagtct aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga
     4741 aaatgggcag aaagaagagg agaaacattt tgatgagcaa ggccaattaa atggtctggt
     4801 taagcaatgg tataaaaatg gtaatcttgc aaaatcacaa aactataaac atgacatact
     4861 cgatggtaac agtgaagagt ggtacgaaaa tggcattcct gaaagtttgt acccttataa
     4921 gaatggaaaa accgacggca ttgccaaatc atggaataag tatggtaaat taacctattc
     4981 aatcgaatac aaaaatagcg tagagaatgg ggcatatcgt aactggagca aaaacacagg
     5041 taaactaacc aaagaaaccc tgtacgtcaa tggtatcaga cagggagtgg aaaaagagtt
     5101 taacgatcgc acaggtaaac tattaacctc cacgcaatat gttaataata aaagacatgg
     5161 tacggaggaa acttatgatc aaaacggaat aaaatatatt acatgctatc aaaatgatca
     5221 gaaattatcg tctttagaca atccaacaca aataaaagat aatgcaacaa caggcgattc
     5281 cagtgctcaa tttgcattag ggaaatacga attcatctgt gcaaatatag atgaagggat
     5341 taaatggtta accaaatctg cagagcaaaa aaaacactga tgcgatatac tttttagcta
     5401 cagcctacaa a
//
LOCUS       DADLTI010000225         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLTI010000225 DADLTI010000000
VERSION     DADLTI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.228"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN755_003903"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN755_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7382511.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JN755_003904"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JN755_003904"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7382512.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYKAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JN755_003905"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JN755_003905"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7382513.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JN755_003906"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JN755_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7382514.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1455 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtataaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLTI010000226         5318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLTI010000226 DADLTI010000000
VERSION     DADLTI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.229"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..607
                     /locus_tag="JN755_003907"
                     /pseudo
     CDS             1..607
                     /locus_tag="JN755_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            671..1597
                     /gene="glsB"
                     /locus_tag="JN755_003908"
     CDS             671..1597
                     /gene="glsB"
                     /locus_tag="JN755_003908"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7382515.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1564..1956
                     /locus_tag="JN755_003909"
     CDS             1564..1956
                     /locus_tag="JN755_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7382516.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2095..3513
                     /locus_tag="JN755_003910"
     CDS             2095..3513
                     /locus_tag="JN755_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7382517.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3740..5191
                     /locus_tag="JN755_003911"
     CDS             3740..5191
                     /locus_tag="JN755_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7382518.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1228 a         1238 c         1433 g         1419 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gctattggcg cacaggctgg agcggcactg
      241 aaaaaatgcg tactactatc cgccaacggt tctggcgaat gttaccccag aaatgaccgc
      301 gtttcgggaa gaaatgtttg gccccgttgc tgcaatcacc attgcgaaag atgcagaaca
      361 tgcgctggaa ctggctaatg atagtgagtt cggcctttca gcgaccattt ttaccaccga
      421 cgaaacacag gccagacaga tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg
      481 ttattgtgcc agcgacgcgt gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg
      541 tgagctttcc catttcggct tacacgaatt ctgtaatatc cagacagtgt ggaaagaccg
      601 gatctgacgc gacatattca gctctgatat actcgcaggt cttttcagac ctgcggtcca
      661 ggagtagaaa gtggcagtcg ccatggataa tgcaatttta gaaaacatct tgcggcaagt
      721 gcggccgctc attggtcagg gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga
      781 cggttcccga ttggggattg ctatctgtac cgttgacgga cagctttttc aggccggaga
      841 cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat
      901 gcgtcattac tccgaagagg aaatctggca acgcgtcggc aaagatccgt ctggatcacc
      961 gttcaattct ttagtgcaac tggaaatgga acagggtatt ccgcgtaatc cgttcattaa
     1021 tgccggtgcg ctggtggtct gcgatatgtt gcaagggcga ctaagcgcac cacggcaacg
     1081 tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg atacggtggt
     1141 agcgcgttcc gaatttgaac attccgcgcg aaatgcggct atcgcttggc tgatgaagtc
     1201 gtttggcaat ttccatcatg acgtgacaac cgttctgcaa aactactttc attactgcgc
     1261 tctgaaaatg agctgtgtag agctggcccg gacgtttgtc tttctggcta atcaggggaa
     1321 agctattcat attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct
     1381 gatggcgacc agtggtatgt accagaacgc gggggagttt gcctggcggg tgggactacc
     1441 ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc
     1501 agtctggagt ccggaactgg atgatgcagg taactcgctt gcgggtatcg ccgtccttga
     1561 acaattgacg aaacagttag ggcgttcggt ttattaaggc agtctctcga tccactcttc
     1621 gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa agagcgtcag
     1681 tattgcctgg agaaaggcgc gccagtcatc gaacaacatg cggcggattt tgtcgctaaa
     1741 cgtcttgctc cggcattacc ggctaatgac ggtaagcaac cccccatgcg cgggcatccg
     1801 gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat
     1861 aacattccgc agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc
     1921 tatcactggc tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag
     1981 cattcccagg gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa
     2041 tttacttatc acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat
     2101 gtacacccca tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc
     2161 gctatttttg cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac
     2221 gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc
     2281 tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc
     2341 ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt
     2401 gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca
     2461 gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc
     2521 ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg
     2581 ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt
     2641 cctggagtgc tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc gcttattgtg
     2701 tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca
     2761 ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc
     2821 ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg
     2881 ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca
     2941 attatggttt cttttagcgt ggcagcgatc aatttgctaa tgaagcaagt tgcgctgcga
     3001 gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga ggcgctgaaa
     3061 agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat cgactatttc
     3121 aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc
     3181 cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa
     3241 tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc
     3301 cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta
     3361 agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa
     3421 ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc
     3481 agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga
     3541 atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat
     3601 gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaaca
     3661 ggggttcggc gtattaaagt ttgctaacct gtcgcaccaa ttcaaacagg ttgtatgact
     3721 aatcagaagg gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta
     3781 tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca
     3841 aatcgatctc ctgaatgagc acaccgatct gaattctggc gtggtcgttg ttcgtccgat
     3901 tgaaacttca ttcccgccgt cactgagcac gcaggatggt ctgtacacca ccattatccg
     3961 tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg
     4021 tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggcgcaca acccggaaat
     4081 gcgctttgtt ttctctaaca ccaccgaagc gggtatcagc taccatgcgg gcgataaatt
     4141 cgatgacgcg ccagcggtaa gctatccggc aaaactgacc cgtctgctgt tcgaacgctt
     4201 tagccatttc aacggtgcgc tggataaagg ttggatcatc attccgtgtg agttgattga
     4261 ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc
     4321 agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg
     4381 tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagaac tgggttatca
     4441 cgatggtttc ctcgacaccg ctgaacattt ttacctgttt gtgattcagg ggccgaaatc
     4501 cttagcgact gagctgcgcc tggacaaata tccgctcaac gtgctgattg ttgacgatat
     4561 taaaccgtat aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc
     4621 ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg
     4681 cgcattcgtt gaaaaagcca tctacgaaga aattattccg gtactggatt tgcctcgtga
     4741 tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca
     4801 tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcacccgca tcctgccgca
     4861 gctgctggca gggcagaagg caaacggcac acttccggcg cgccttactt tcgcattagc
     4921 ggcattgatt gcgttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga
     4981 tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg
     5041 tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac
     5101 gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg
     5161 gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg
     5221 accgggtttc ttatttcact atattagtta caacatccta tatgccttta tcattaacag
     5281 gtgtcacatg cagcgcctgt taatgttgta agcttgac
//
LOCUS       DADLTI010000227         5128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLTI010000227 DADLTI010000000
VERSION     DADLTI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.230"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(109..1074)
                     /locus_tag="JN755_003912"
     CDS             complement(109..1074)
                     /locus_tag="JN755_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7382519.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
     gene            complement(1529..2749)
                     /locus_tag="JN755_003913"
                     /pseudo
     CDS             complement(1529..2749)
                     /locus_tag="JN755_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            2850..3275
                     /gene="umuD"
                     /locus_tag="JN755_003914"
     CDS             2850..3275
                     /gene="umuD"
                     /locus_tag="JN755_003914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7382520.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3275..4546
                     /locus_tag="JN755_003915"
     CDS             3275..4546
                     /locus_tag="JN755_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7382521.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            4625..4876
                     /locus_tag="JN755_003916"
     CDS             4625..4876
                     /locus_tag="JN755_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7382522.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            4930..>5128
                     /locus_tag="JN755_003917"
     CDS             4930..>5128
                     /locus_tag="JN755_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382523.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGD"
BASE COUNT         1143 a         1413 c         1373 g         1199 t
ORIGIN      
        1 gaggccggcc gcgttacaga attctaaaaa acagtgactt atataattcc ctccctgatt
       61 ctctctcttt caaaaattct gaagccctgc accgcagggc tccagctgtc agcagtccat
      121 cagccagcgg tgcgcttttg aggccgctgc cgccgcttta aagatgtagc gcttgtctcc
      181 cttcagcgct ttcagccagg acgcgatata gctttcatgc tgcacctctc ccgtaatccc
      241 caaatccgcc aacagaaacg cgctgcccag ctcggcgatc agttcctcaa acgcatacgc
      301 ctcagagcca aacttattgc ctttttcacg attgaggcgg gacgctgcac ccgtccagtg
      361 aaccagctca tgcaggccgg tggcgtagaa gttcgccgcg tccgcgaagc ggaaccgctc
      421 cggcaggacg atttcatcgg tggccggacg atagaacgcc tttacgccct gctcggtgat
      481 ttttgccccg ctccggatca tgagtgcttc cgcctgcggc agcggatcga aaccggcaac
      541 cggctgcggc acgctctcga tgctcaggcc gtcgatttgc tccacgttaa acacggtaaa
      601 ggctttcagc atcgggattt tttcgatttc cccgtcctcg tcctctttct ccagcgtctt
      661 gtaaaagatg gccgtcgtgc cgtgttcacc tttacgaacc tggccgccca gctcctgcgc
      721 ctgtttgtag gtcagccagc gctcgtcctg aaaaccctgt tttgccgcac tacaccacag
      781 cagcatgata ttaattccgc tgtatgccgt gccggtgctg aagttggccg gtaatcccac
      841 cgctgcgccg ttgcgaaccc acggacacac ccacggctta acgccgcttt ccagggctgc
      901 aatgatgctg tccgttactg tctggtaaag gtctgttttg actgcgccag cacgggcttt
      961 gcttttgccc ctggaaaatt tcgtttttga ggagcctgac tgctccagcg gggatacgct
     1021 ggtcgcctgt gaagctttag gggcggttgt ctgagtgtgc aggttcatcg tcatggctgt
     1081 ttctccgtca gtgtgaattt cgtcttcgta tcgcctgacg gttcggcctg cccggagtcg
     1141 ttctggtcag caagggcgcg cagcgtgcca tttacccttg ctgtccaggt tgtgacgggg
     1201 gacgattacc ggaagctgat acgcaggaga aaggcatcac aggagaaaat gacgatgtgc
     1261 acacccggac ttaccgcccc tttagctgca cgggtgaaac tgcccttcag cgtccgggag
     1321 gacgggtaaa aaaagcactg acgacgtcag tgctgccttt gtgacatgcg gtgttacact
     1381 gcctctgccg gtcagttcgc ccttcctgaa accgggggcg gtttgttttc ggggacgttc
     1441 tgaccggcta tccccccatc acttgcagaa atttcaccgc cccttatact gtatgcatat
     1501 gggtcttccc cgatcatggt gggaaggctc agaacgccat attcagcttt ccgtagtgga
     1561 acatcacccc cagtttaaag cgctcccggt ttcggtatcc cctggctttt atcctcagca
     1621 gcctgatctt gctgttaagt gcctccgcat ttccgtttga gacactgtgt cgcatcgcat
     1681 tcaggatccc ctacagcctt tttcctatcg ttttcgcggc atttttcatc atgggaacgt
     1741 cactgttagc cgccagcgcc aaccatctct gccagtcact ccgtctttcc tcgctccatg
     1801 gcctgttcca gatatccttt gccagctctt tcagcgccca gcactggctc gtcagcttca
     1861 tctgtgcacg cagccacatc agcttttcct gccgggattc ggtcatccac ttatcgctgt
     1921 actgccacag gaagcgggtt ccttttgcct ggtgtcggct ttcaacaggg aggtgcggat
     1981 gttcattctg acgggtttta tcaactacct cgcccagttg cttcgccaca tggaagcggt
     2041 caaaggcgat tttctcaacc gcactgggta agtggatacg cgctgctctt atatagcccg
     2101 cgttcatgtc cattgagagc gttttgatag ccagcaactg cccatcagtg agcgtgcgaa
     2161 gatagccggc aagactctct gtgccgcgat catccgttaa ggccagcgcc cgaccatcgc
     2221 gatcggagat caccgttatg taacgatgtc cttttttaaa ggcgacctca tccacattca
     2281 tatgacgggc ggataatggc ttttttatcc gggcaagacc tcgcttaact gcccgggtca
     2341 taatgccgtc aaccgcattc caactgagct taagttgctt cctgacagca tcaacggtgc
     2401 tgattttcag ccatgagaga acgaacgatt cgaatagcaa cgtataccgg cttccggggc
     2461 cagcccacgg aacaggcaac gtcaggcagc catgctccgg acacataatt cgtggaacat
     2521 cggcttcaac aatagtggtg aactggcagg tatcaagatg gcgccattta cgatgacggt
     2581 gatcgtgaac agaacaggat ttaccgcagg tcggacaggc tagccgggtg ttttcagcga
     2641 tctcaatagt gacagtaaca gaaccggcat tttcatcgag agaaagggac tttacctgcc
     2701 acggatcgga caggttgaga atatgagcgt agagggactt ttcgtccatg gcggtgacct
     2761 ctggcgatta aatacaccat tatcatgcct tcagccacca caacaaggga agacccatgc
     2821 atatacagtt ttaagcgagg gaaaaaacga tgaagttcat tcctgcagta tcagaagcca
     2881 gcatagtaaa aattcctttg ttcacagagc gatgccctgc gggttttccg tcaccggcag
     2941 caaactatac tgagtctgaa ctggatctga atgagtactg cattcaccgg cgccactcca
     3001 cctattttgt cagagcgatt ggcaactcaa tgactgatat tggcctctat tccggcgatc
     3061 tgatggtcgt ggataaagca gaacagccgc ggcacgggga tattgttatc gcggaaattg
     3121 agggtgaatt tacggtaaaa cgcctgcttc tgacgccgcg gccagccctg caggcgatga
     3181 acccggactt cccttccctg tacccggatc cggaaactct gcaaattttc ggcgtggtga
     3241 cggccttcat tcataaaacc cggagggcag actgatgttc gccctggcag acgtcaactc
     3301 cttctacgcc agttgcgaga aggttttcag gccggatttg cgcggcaaac ctgtcgtcgt
     3361 tctcagtaac aacgatggct gcgttattgc ccggagtgct gaagccaaac ggctgggtat
     3421 aaagatggga accccctggt tccagctcaa agaggcgcag tttcctgaaa aactgtacgt
     3481 tttttccagc aactacgagc tctacgcgag cctgagtaac cgtgtggtcg ccctgctgga
     3541 agagctgtcg ccccgcgtcg aacaatactc aattgacgaa tgtttcctgg atgcccgggg
     3601 aattagccac tgtatggatc tggaggattt tggcaggcag ctgcgtgggc atgttctcag
     3661 tggaaccgga ctgacgatcg gtgtcggctt cggcgccaca aaaacactgg ccaaatcggc
     3721 gcagtgggcg tcaaaggaat ggccacaatt cagtggggtg ctggcgctgt cgcccgaaaa
     3781 tccacgcaga acggcaaaat tactcagcct gcagccggtg gaagaaatct ggggcgtggg
     3841 gaaccggata gcgaaaaagc tgcacgttat ggggatcacc accgccctgc agctgtcgct
     3901 gacgaaccct acatttatcc ggaaaaactt caacgtagtg ctggagcgca cggtacgtga
     3961 gctgaacggc gagagttgca tttcgctgga ggaagcgccg ccgccgaaac agcagattgt
     4021 ctgcagtcgc agcttcgggc agcggatcac cacctacgga gaaatgcgcc aggccgtctg
     4081 ccagtatgcc gagcgcgcgg cggaaaagtt gcgcggtgag cgacagtact gccggcatat
     4141 cagcaccttc atcaagacat ccccttttgc ggttaatgag ccgtattacg gtaacgtggc
     4201 cacggaaaaa ctgaacaccc ctacccgtga cacccgggac attatcgcgg cagcggtcag
     4261 gtctctggac aggatatggc tcgatggcca ccgctacgca aaagcgggga taatgctcaa
     4321 tgatttcagt ccgaacggcg tcgcgcagct gaatctgttc gatgacgtgc agccacggcc
     4381 gcacagcgat gcgctgatga aggtactcga cggcattaat cattccggac tcgggaaagt
     4441 ctggtttgcc ggacggggta ttgcgccgga ctggcagatg aaacgggaga tgctgtcacc
     4501 ggcatatacc acacgctgga aggagctgcc ggttgcccgt ttctgaccgt tgaaatgaca
     4561 gaagggactt gccgccagag cttactttac cgatcataca ggtgacttgt ctcagggggg
     4621 aaccatgaca aatatcattc tgtgtgatgt gactgccagc gtcagcgaac ttaaaaacga
     4681 tccggtggcc accgccagtg cccgcggcgg ctacccggtc gagataattg accgtaaccg
     4741 ccctgttttt tattgtgtgc ccgcggcttt gtatgaacaa atgctcgatg agctggacga
     4801 aaaagacctg gtgcaaacga taacagaacg tcaaaaccag ccgctgcgtg aagtggatct
     4861 caatcagtat ctgtgatgtg ctccggtgcg aggagcaagg aatgtattaa gccaatgtta
     4921 aggaacaata tgggtagcaa aatcgcaggt gtggttttct tatacttcgc tgtctgggga
     4981 atggcttcag tgattgaatt gcagtactgg cgtaacgctg atacctatcc tgtctggctg
     5041 acaaaacatc tccggatctc tcctgaaaat aacgacaaga gcctgataag gatagaaatg
     5101 gaaacctttg acaaggtatg tggtgata
//
LOCUS       DADLTI010000228         5126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLTI010000228 DADLTI010000000
VERSION     DADLTI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.231"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..236
                     /locus_tag="JN755_003918"
     CDS             <1..236
                     /locus_tag="JN755_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382524.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JN755_003919"
                     /pseudo
     CDS             276..816
                     /locus_tag="JN755_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /locus_tag="JN755_003920"
     CDS             883..2301
                     /locus_tag="JN755_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7382525.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYCQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /locus_tag="JN755_003921"
     CDS             2282..2752
                     /locus_tag="JN755_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7382526.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JN755_003922"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JN755_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7382527.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JN755_003923"
     CDS             3834..4751
                     /locus_tag="JN755_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7382528.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JN755_003924"
     CDS             4830..5012
                     /locus_tag="JN755_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7382529.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1227 c         1462 g         1185 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctattgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DADLTI010000229         5020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLTI010000229 DADLTI010000000
VERSION     DADLTI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.232"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JN755_003925"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JN755_003925"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7382530.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JN755_003926"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JN755_003926"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7382531.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JN755_003927"
     CDS             2152..2610
                     /locus_tag="JN755_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7382532.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JN755_003928"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JN755_003928"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7382533.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JN755_003929"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JN755_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7382534.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JN755_003930"
     CDS             3816..4493
                     /locus_tag="JN755_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7382535.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JN755_003931"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JN755_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7382536.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1221 a         1192 c         1321 g         1286 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac ccgaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatt agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tctccagggt aatgactcca acttactgat
//
LOCUS       DADLTI010000230         4983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLTI010000230 DADLTI010000000
VERSION     DADLTI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.233"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..181)
                     /locus_tag="JN755_003932"
     CDS             complement(<1..181)
                     /locus_tag="JN755_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7382537.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JN755_003933"
     CDS             complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JN755_003933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7382538.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JN755_003934"
     CDS             complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JN755_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7382539.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3241..3708
                     /locus_tag="JN755_003935"
     CDS             3241..3708
                     /locus_tag="JN755_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7382540.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3760..4668
                     /gene="rarD"
                     /locus_tag="JN755_003936"
     CDS             3760..4668
                     /gene="rarD"
                     /locus_tag="JN755_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7382541.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4709..>4983
                     /locus_tag="JN755_003937"
     CDS             4709..>4983
                     /locus_tag="JN755_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382542.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGT"
BASE COUNT         1184 a         1286 c         1241 g         1272 t
ORIGIN      
        1 gcaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
       61 ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg
      121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca
      181 tacatttgac tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg
      241 ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac
      301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
      361 tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga
      421 cattactttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
      481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
      541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta
      601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca
      661 cgctcaccca atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat
      721 gaccatagtc gcggatacgc tggttattag caccacgaat cacttccacc acataaccca
      781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac
      841 cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
      901 cttcgccaaa atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc
      961 ccatcgcatt gagtttgtgg cgctcgatat cctgcaactg cccctgcggc ttctcttcca
     1021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca
     1081 ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat
     1141 caaagtggac cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg
     1201 ccaccacaat ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat
     1261 tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg
     1321 tgtcttctac tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct
     1381 gcacgtagcg catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
     1441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
     1501 tatcctggcg cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga
     1561 accgctggcg caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg
     1621 agatacagtg cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa
     1681 gaaagttatc cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc
     1741 ggcagcctgt catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg
     1801 ccgccacgcc gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg
     1861 aaacaaccac ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc
     1921 cgccaccagt gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt
     1981 cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc
     2041 cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa
     2101 acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt
     2161 aatcctggat gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt
     2221 ttagcataac ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc
     2281 catagccgct gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc
     2341 ctaactccgc gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg
     2401 catcaccgag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg
     2461 ggttatcgtc agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt
     2521 tttctgccat caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc
     2581 cgttagagtc gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt
     2641 aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
     2701 actcttcgct attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg
     2761 agtttgggcc taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt
     2821 catccttacg cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc
     2881 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat
     2941 gctcctgcag catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt
     3001 tcaccgttgc ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc
     3061 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt
     3121 tctgcgggct attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga
     3181 aaccaacaac aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat
     3241 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
     3301 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
     3361 cagctggcct ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac
     3421 ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc
     3481 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
     3541 accattgatc ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc
     3601 agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa
     3661 gaacagcttt atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc
     3721 aggtaaactg ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg
     3781 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc
     3841 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
     3901 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
     3961 cagacgccac agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg
     4021 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
     4081 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
     4141 atgcaatggc tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt
     4201 ggttcgctac ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg
     4261 cgcaagaaga ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg
     4321 cccgtggcgg cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa
     4381 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg
     4441 ttgtgtttta ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac
     4501 attggcccga cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc
     4561 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg
     4621 atttatacgc agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg
     4681 actcttgtat gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa
     4741 atttatgtta cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat
     4801 attttcattt attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg
     4861 ggaagcaggt tttcttgtta tagtctctac catcgcactc cattatctcg tcatcaacat
     4921 gaattgccaa cgactccgtg atagtggttt catatatata aaaatttatg tttttggtac
     4981 att
//
LOCUS       DADLTI010000231         4820 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLTI010000231 DADLTI010000000
VERSION     DADLTI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.234"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..437
                     /locus_tag="JN755_003938"
     CDS             <1..437
                     /locus_tag="JN755_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7382543.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JN755_003939"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JN755_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7382544.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="JN755_003940"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="JN755_003940"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7382545.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="JN755_003941"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="JN755_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7382546.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="JN755_003942"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="JN755_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7382547.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..>4820)
                     /gene="metR"
                     /locus_tag="JN755_003943"
     CDS             complement(3923..>4820)
                     /gene="metR"
                     /locus_tag="JN755_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7382548.1"
                     /translation="AAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGE
                     ILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEMD
                     FKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDYEVRLVLAPDHPLAAKTRI
                     TPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAAL
                     PHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPF
                     VKSAERPTYDAPTVRPGSPARL"
BASE COUNT         1054 a         1232 c         1323 g         1211 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg
//
LOCUS       DADLTI010000232         4765 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLTI010000232 DADLTI010000000
VERSION     DADLTI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.235"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            289..819
                     /locus_tag="JN755_003944"
     CDS             289..819
                     /locus_tag="JN755_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7382549.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            826..1059
                     /gene="mbeD"
                     /locus_tag="JN755_003945"
     CDS             826..1059
                     /gene="mbeD"
                     /locus_tag="JN755_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7382550.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1639..2757)
                     /locus_tag="JN755_003946"
     CDS             complement(1639..2757)
                     /locus_tag="JN755_003946"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7382551.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2875..2964)
                     /locus_tag="JN755_003947"
     CDS             complement(2875..2964)
                     /locus_tag="JN755_003947"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382552.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3400..3561)
                     /locus_tag="JN755_003948"
     CDS             complement(3400..3561)
                     /locus_tag="JN755_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7382553.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4213..4404)
                     /locus_tag="JN755_003949"
     CDS             complement(4213..4404)
                     /locus_tag="JN755_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7382554.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4444..4698
                     /locus_tag="JN755_003950"
     CDS             4444..4698
                     /locus_tag="JN755_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7382555.1"
                     /translation="MIPMKRERMLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPV
                     ARSGKLPTLAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
BASE COUNT         1330 a          987 c         1204 g         1244 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg gatagacgcg gtttaccatg ccggagagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggattgacg tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggcggg aagaatgtca gactgaaagg agcgttttat gagcaatctt
      301 ttacagacgg gtgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa acagggaact gatctcaaac
      421 gaatctttaa tcaggaacgc tatcaacgac catacatcag ccctgaaaga acttctggag
      481 aggtatcaga aaacgaccgt cgatacgatg gacgcacact ggaaaaccgt actgaaaatg
      541 agcgtgaaac gctggctgtg gctgattatc gtctcagtac tgatgtttgc cacgaccggg
      601 agcctgctgt ggtgccaggg catgaagata aacgcgaaca tgaatattct ccgcgagcag
      661 aaagagagtc tggagaagct gaacgcgaag acatggggcg tgcgttatca cgaggacagc
      721 aacggacgtt ttctggtgtt gcccgagggg atgaaagccg aaacgaactg gacgatggaa
      781 aacgggaaac agaatgccgt aaggctggta caggagtaac agagcatgac ggagctggaa
      841 agacatttgc tgaacgcatt agagcaactg caacaggact atatgcagcg gctgaacgaa
      901 tgggagagcg ccttcgtgga attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg
      961 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcagaca
     1021 gaacgcttga gccagttata cagcgagaac aggagataag ggacgaacgg ctgatacagg
     1081 aatgcgaaca tgcgttatcc ctggagcgtg agcgacagcc ggaaatacag gaacggacac
     1141 tggatggccc ttcgctggga tggtaatcag agacaggcta aaatgttgtc agcgcgtttc
     1201 cggtgcattt tttgggataa tggttggttt ggggggcacc gggaacgcgc ttcagtcaga
     1261 ccttgctgaa aacataggaa cgcgttaaaa cgcatacggg cgcgattctg gtgtgtcttt
     1321 tgttgctgtg ttttgcctct attgccagat cgcgctcatt cccctgaact tctgttttcc
     1381 cgctcaggtc cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt tcagctgtct
     1441 ctggctggca attcctgcgt gattcacatg gctgcatagc tatgcatgtg gttaaaattt
     1501 accgggtgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc
     1561 cgtgatcgcg ccgaacgcgt ttcagcggtg cgtacaatga gatgttatgg taaattcaag
     1621 tcttagattt tatgatgttt aattagcttt ttttgcgttt ataatgttca tcttttttat
     1681 taaaattgat tcaatctttg atgtgggtat taatatttta tttgacaaat agaaagttaa
     1741 aaaaaacacc attaatagta taaataaata cccaaaaaca gaagcgttat taatttcaat
     1801 tattgatgtt aatctgtata ttactacatg ctgaatcaac atggatatgt agatgcattt
     1861 agcatatgag ttgaaaaaat agaggttaaa tttcttatta attattaatg ctgctttgta
     1921 aatgataatg cttatgcaca ttaaaggtat tattgataac tggtgattga ctatgtttgt
     1981 tttgaactca ttaggcccta ttaataatac tgtcaatata catataacaa ccgatagaat
     2041 aactgtggtg tttttttgta atttattttt gtatttaaat gcaagcatcc caaccataaa
     2101 ctcaggaagt cggcaagcaa aaaaccaaaa tgggaagttt aaaaatgatg atgagatatt
     2161 tgatatcggg aatgttgtaa ttgttatcac aaataaaaaa aatatgttac gtttaaatgt
     2221 aaaggtattc ataacgtgaa aaactatcgg tgatatcacg tacagcataa taacgcaccc
     2281 aataaaccac tcgcctgtaa ggaaaagaaa atcataagag aaatgggcgt ttaatatacc
     2341 atcaagggca aaaaatactg gaattattct agtgtactgg cctcctgttg tcattattga
     2401 tacgagaggg actgtattaa ttatctcttt atttaaataa gacaataaaa ttaagagtaa
     2461 agagataaaa aagtaagtga taatataagg agtaaggatc cttactcctt tgtcaaggaa
     2521 ataatcaatg gtattctttc tatctagtga aataaatgcc aaatatccag atataaaaaa
     2581 aaacaagctc acacctacct ggccaagaac ccctccataa aatgatggta tgaaagatag
     2641 cctggtttgg ctaaatatgt agccataatg agatataata acaaaaatgc aggctgttac
     2701 tcgcaatgta tcaattgacg gatctctatt agacataacc tttatttcgc tgttcaaatt
     2761 atcgccttta gttgatgatg taaaattaac gctataaata agctacacat tctagagcag
     2821 cgaaaggatg attcaagtat ttttaataat aaaacgcatt gataatgttt atgttcagat
     2881 ggtgctgata gctttgtcat gctgttccac tgatgttgat aacgacagag acttttgtcc
     2941 ttttcttgtt agatgctgcc tcagattcgg attatggcgt taaaggggtt tgacgctcaa
     3001 ccgaacggtt tcacacgtta agagcttttt gtctgataaa tcaaaatata atggacattg
     3061 aaaaacgccc tcaaaacggt gtctttttaa tcgaagattg gcacaaaacg gacaccaccg
     3121 tccattagtc gattttttag acggcttttc tcgcctaacg tgagttttcg ttcggttgag
     3181 catcagaccc cttttatatt gtagatttct atttttagac agttggaaca ggacaattaa
     3241 caacagatct acttgagatc atttgcttgt cgtcgtagtc tctttcccag aaacgaaaaa
     3301 accgccctgc aaggcggttt ctcgaaggtt ctcagagcga ccaactcttt gaaccaaggt
     3361 aactggctta tgaggagcgc agtaaccaaa tctgtccttt cagtttagcc ttaacaggcg
     3421 cataacttca agactaactc ctctaaatcc gttaccaatg gctaccgcca ctggcgattt
     3481 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gaacgggcgt ggtagtcgga
     3541 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     3601 tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     3661 aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttata
     3721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg
     3781 ggcggagcct atggaaaaac gccatcggcg cggccttctt cctgtttctg gtcttttgct
     3841 cacatgttct ttccggcgtt atcccctgat tctgtggata acagcatttc gtttttgagt
     3901 gagtagatac cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
     3961 cggaaaagcg cctggacgtg cattttctcc ttacgcatct gtgcggcatt tcacacccgg
     4021 tatggcgcac ttttcataca atctgcactg atgccgcatg gttaagcccg tatacactcc
     4081 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgctaaaac ctgctgacgc
     4141 gccctgacgg gcttgtctgc tcccggcatc cgctcacaga caagctgtga ccgtctccgg
     4201 gagctgcata tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta
     4261 aagctcatcg gcgtggtcgt gaagcgattc acagatatca gcctgttcat ccgcgtccag
     4321 ttcgttgagc ttctccagca gcgttaatgt ctgccttctg ataaagcggg ccatgttaag
     4381 ggcggttttt tcctgtttag tcactgatac ctccgtgtaa gggggatttc tgttcatgag
     4441 gtaatgatac cgatgaaacg cgagaggatg ctcacgatac gggttactga tgatgaacat
     4501 gtccggttac tggaacgctg tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc
     4561 ctgggtgagc cggttgcccg ttccgggaaa ctgccgacac tggcaccgcc gttactgcgt
     4621 cagctggctg ccatcgggga cgagctgcgc cggctgcgtc tggccgtcag ggaacagggg
     4681 acgcgggatg atagttaagt ttcatgcccg gggaaaaggt ggtggcagtg gtccggttga
     4741 ttacctgctg ggaagggagc gtaac
//
LOCUS       DADLTI010000233         4666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLTI010000233 DADLTI010000000
VERSION     DADLTI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.236"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            111..2997
                     /locus_tag="JN755_003951"
                     /pseudo
     CDS             111..2997
                     /locus_tag="JN755_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3107..3955)
                     /gene="alkA"
                     /locus_tag="JN755_003952"
     CDS             complement(3107..3955)
                     /gene="alkA"
                     /locus_tag="JN755_003952"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7382556.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4089..>4666
                     /gene="yegD"
                     /locus_tag="JN755_003953"
     CDS             4089..>4666
                     /gene="yegD"
                     /locus_tag="JN755_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7382557.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQY"
BASE COUNT         1091 a         1218 c         1261 g         1096 t
ORIGIN      
        1 accagacccg gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg
       61 gtctggctgg ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc
      121 tgctgacgcc cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca
      181 tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc
      241 atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa
      301 gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt
      361 ggagcgccgt gcgcctgcca cgaatggaag cctttttgat cttccttacc acggtgatga
      421 tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga
      481 tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca
      541 tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc
      601 ggaacgcgat ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc
      661 tgcaatccaa caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac
      721 tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa
      781 agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg
      841 gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc
      901 tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc
      961 agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt
     1021 gggagctgaa gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc
     1081 ctccgcatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc
     1141 agcatgccga aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     1201 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     1261 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
     1321 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
     1381 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat
     1441 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
     1501 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     1561 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
     1621 tattacgccg ttaagtactc tggacggcag caatataggc tctgtactgg tgattcagga
     1681 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     1741 gacgcatctc gccaatcgcg ccaattttga gaagcaactg cgtatcctgc tgcaaacggt
     1801 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     1861 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
     1921 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
     1981 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
     2041 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     2101 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     2161 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggttacga accacagcaa
     2221 gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa
     2281 gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt
     2341 aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat tgatgaacaa
     2401 aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc
     2461 cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag catcgccctc
     2521 cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg
     2581 gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga agcgatttta
     2641 gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt gctcagccag
     2701 gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta
     2761 cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg
     2821 attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta
     2881 cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt
     2941 gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgagag
     3001 agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac aaatcgctca
     3061 ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat gcttcgtctg
     3121 gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc
     3181 gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt ttaatcagat
     3241 aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa taattcgccg
     3301 tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg
     3361 gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc agcgcctctg
     3421 cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct gccagccgct
     3481 gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgtg
     3541 ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc
     3601 gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc cgcgccgcgc
     3661 ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca agcaggcggc
     3721 tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca
     3781 gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg cccactgcca
     3841 gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca cgggcggcga
     3901 gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata tacatcgcat
     3961 ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt gctttcatat
     4021 tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg
     4081 attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc
     4141 gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg ccttcaatgc
     4201 tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat gacgttccgg
     4261 cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac cgcgaagaag
     4321 atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg
     4381 atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct
     4441 taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc
     4501 gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt ggtcgcccga
     4561 tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg attctggaac
     4621 gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacga
//
LOCUS       DADLTI010000234         4613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLTI010000234 DADLTI010000000
VERSION     DADLTI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.237"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..213
                     /locus_tag="JN755_003954"
     CDS             <1..213
                     /locus_tag="JN755_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7382558.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JN755_003955"
                     /pseudo
     CDS             356..577
                     /locus_tag="JN755_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JN755_003956"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JN755_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JN755_003957"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JN755_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7382559.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JN755_003958"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JN755_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JN755_003959"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JN755_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7382560.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1611 a          823 c          859 g         1320 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg tta
//
LOCUS       DADLTI010000235         4581 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLTI010000235 DADLTI010000000
VERSION     DADLTI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.238"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(245..1031)
                     /locus_tag="JN755_003960"
                     /pseudo
     CDS             complement(245..1031)
                     /locus_tag="JN755_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1350..2627
                     /locus_tag="JN755_003961"
     CDS             1350..2627
                     /locus_tag="JN755_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382561.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2654..4124
                     /locus_tag="JN755_003962"
                     /pseudo
     CDS             2654..4124
                     /locus_tag="JN755_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         1233 a         1031 c         1085 g         1232 t
ORIGIN      
        1 aaccgagttt tacgaccgct gccggaatcg gcaaagtata aatgccatga atatccggaa
       61 atttacgaaa acggtcaata ctgtttttct ttaatactgt ttcatcggta attacgcgat
      121 ctgcaccaac aatttccttt agctggtcga caatcgctgc gcgagataaa gacatagtaa
      181 ttccttcctg ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta
      241 tggattagcg cactaaataa ccgccatcga ccactaataa atgcccgttg acataattcg
      301 atgcctgact tgcgagaaac acggctgcgc ccattaaatc ctgagtatcg ccccaacggt
      361 ttgccggaat atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagca
      421 taatatgctg ttgcataata gccaggggcg ataccattta cctgaatatt atattgacct
      481 agttcatcac aataagcttt ggtgaacccg gcaagagcat gtttagtggc agaatatgca
      541 ggtgaccatt gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt
      601 ttctgcggga tcataatttt tgccgcttca tagcttaact cgaatgcggc ggtcaggttc
      661 acatcaatca tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa
      721 ataccggcat tgttaaccag aatatcgaca gtaccgaagc gctcacagca ggcagcgata
      781 atcttctgcg gtgcgccttc tgcggtaata tccacctgca tgaagtccac ttcaacaccc
      841 tgtttttcaa tcatttcctt tgtttcgccg ttatctttga cgaagctagg aataaaaata
      901 tttgcgccag ctttggccaa cgccattgca aatgcctgac ctaaaccgct attcccgccg
      961 gtaacaattg ctgttttacc tttcagggag aaaaaatcca ttgagaacgc attgagagat
     1021 tcgattgaca tagttagctc caatttccgt aaggcaaaaa aaaagaaagg taacttcccc
     1081 cgcgagaggg aagttacctt tctcattatc tctggtaact ggagataatt tttagcataa
     1141 cggcataact gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt
     1201 aaaaagcaga agcgtgatta ctctcacaca acacctctgc tttccatgtt atttcttatt
     1261 gcaattacta gcgacctgag aaaagtaatc cacctgaaag ggcggcttac ttttctcttt
     1321 tttttgtcta ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc cgttggataa
     1381 ccctcgcgat aattagtttt agcggcggcg ttagttttga cctggcttat ttacgttata
     1441 tttatcaaat tcccatggcg aaatttatgg gattcagcaa taccgagata ggtttaataa
     1501 tgagtacctt tggtattgcg gctattattc tttatgcccc cagcggcgtt attgccgata
     1561 aattttcaca ccgcaaaatg attacttccg cgatgatcat taccggatta ctgggtctgt
     1621 taatggcaac gtatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa
     1681 cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctt ggcgatcata
     1741 gcgagcaagg gaaaattatg ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc
     1801 tggcggtgtt taccatgtgg gtcttttctc gctttgcacc ggatgacagc gccagcctga
     1861 aaacagtcat tatcatctac agtgtggttt acatcttgtt ggggattctg tgctggtttt
     1921 ttgttagcga taacaacaac ctgcgcagtg ccaataacga agaaaaacag tcattccagc
     1981 ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg gtattgcagc atggtgattt
     2041 ttggtgtctt cacaatctac gccattctga gttactccac caactatctg accgaaatgt
     2101 acggcatgtc gctggtggca gcgagctaca tggggattgt gatcaacaaa atcttccgcg
     2161 cgctgtgcgg cccacttgga ggcattatca ccacctacag taaagtgaaa tcccctaccc
     2221 gcgtgattca aatcctttcc atcatcggtt tgctggcgtt gacagccctg ctcgtcacga
     2281 actctaaccc gcaatcggtc gcgatgggga ttggcctgat tttactgctg ggattcacct
     2341 gttacgcctc acgcgggctg tactgggcct gccccggcga agcgagaaca ccgtcttaca
     2401 ttatgggcac cacggtaggt atttgttcgg tgattggatt cctgccggat gtcttcgttt
     2461 acccgattat cggccactgg caagacaccc tgcccgctgc agaagcctac cgcaatatgt
     2521 ggctgatggg catggcggcg cttgccatgg tgatagtctt tacctttttg ctgttccaaa
     2581 aaattcgtac tgctgatagc gcccccgcaa tggctagcag caagtaagcc aggaaaatta
     2641 caggagaaaa atcatgtcga aaaaatacat catagggatt gatggcggaa gtcagagcac
     2701 aaaagtggtg atatacgatc tggaaggtaa tgtggtttgc gaagggaaag gcttattgca
     2761 gccgatgcac acgccagatg ccgatactgc agaacatcct gacgacgatt tatgggcatc
     2821 attatgtttt gccggtcacg atttgatgag tcagtttgcc gggaataaag aagatattgt
     2881 cggtattggt ctgggatcca tccgttgctg ccgtgcgtta ttgaaagccg atggcacggc
     2941 cgttgattag ctggcaggat gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg
     3001 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
     3061 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg
     3121 aagatgctgc ggtaatggag aagtttaata tcccccgtca tatactgttt gatgtgcaaa
     3181 tgccaggcac tgttcttggg catatcacac cacaagccgc gctggcgaca catttcccag
     3241 ctgggctgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
     3301 tactggatga tgaaacggcg gtgatttcac tcggtactta tatcgcgctg atgatgaacg
     3361 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
     3421 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg
     3481 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
     3541 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
     3601 attggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
     3661 gcatggatta cgcatggata tatcgttcga tactggaaag cgtggcgctg acgctgaaga
     3721 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
     3781 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
     3841 cacgacgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acagcggtag
     3901 gtctggggct atacccggat tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata
     3961 tctttatgcc tgttgagagc aatgccaaac gctacgacgc gatgaataaa ggtattttca
     4021 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
     4081 aattggggaa tgcggattcg atccagagct ggtcgaatgc gtaagtgagg aagaccgggc
     4141 gggaaactgc ccggcctgaa tatacctgaa tggttatccc cgctgacgcg gggaactcga
     4201 ataactgacc aacgtctggc tctctaaatc cggtttatcc ccgctggcgc ggggaacact
     4261 ctaaacataa cctattatta attaatgatt ttttaagcca gtcacaatct accaacttta
     4321 caggatcaca caaacaacac atccattatg ttaaagagca cttaatccat tgattaaaaa
     4381 ggtaaatatt taaaataact ctatacaaac taaaatctac caaacttcgc cgcaataatt
     4441 ttcactccag cgaaaaatta acgccgcaga atttgtagag aatgataatg attagtccgg
     4501 gatgggcaag atggatgagt tttcaggggc gacaaataat acaaatgagt ggaatttttt
     4561 acaagttcca cccatttata a
//
LOCUS       DADLTI010000236         4555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLTI010000236 DADLTI010000000
VERSION     DADLTI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.239"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(75..329)
                     /locus_tag="JN755_003963"
     CDS             complement(75..329)
                     /locus_tag="JN755_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7382562.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            639..2411
                     /gene="aspS"
                     /locus_tag="JN755_003964"
     CDS             639..2411
                     /gene="aspS"
                     /locus_tag="JN755_003964"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7382563.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2529..2981
                     /gene="nudB"
                     /locus_tag="JN755_003965"
     CDS             2529..2981
                     /gene="nudB"
                     /locus_tag="JN755_003965"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7382564.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3010..3750
                     /locus_tag="JN755_003966"
     CDS             3010..3750
                     /locus_tag="JN755_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7382565.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3785..4306
                     /gene="ruvC"
                     /locus_tag="JN755_003967"
     CDS             3785..4306
                     /gene="ruvC"
                     /locus_tag="JN755_003967"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7382566.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4308..>4555)
                     /locus_tag="JN755_003968"
     CDS             complement(4308..>4555)
                     /locus_tag="JN755_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382567.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1129 a         1141 c         1266 g         1019 t
ORIGIN      
        1 ccccaccaga cccggtgaca tcaccccccg gatcatggac gactgggggc agtaatcatg
       61 ccgggtctgg ctggctaaca ccccattata aggggtacga ataaagtaac gggggcatca
      121 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
      181 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
      241 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
      301 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
      361 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
      421 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
      481 acgaggcgtt ccagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
      541 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
      601 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
      661 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
      721 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
      781 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
      841 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
      901 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
      961 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     1021 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     1081 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     1141 ccacgccgga aggcgcgcgt gactacctgg tgccttctcg tgtgcacaaa ggtaaattct
     1201 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     1261 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     1321 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     1381 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     1441 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     1501 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     1561 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     1621 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     1681 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     1741 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     1801 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gggatgggcg
     1861 cactgctcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     1921 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     1981 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     2041 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     2101 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     2161 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     2221 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     2281 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     2341 cgagctttgc taacccgact gcactggctg agctgagcat tcaggttgtg aagaaggctg
     2401 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     2461 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     2521 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     2581 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     2641 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     2701 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     2761 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     2821 atacggaatc atggttctgt cttgcgcttc cgcacgagcg gcagatcgtt ttcactgaac
     2881 atctggctta caagtggctt gatgcgcctg ctgcggcggc gctcactaag tcctggagca
     2941 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     3001 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     3061 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     3121 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     3181 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     3241 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     3301 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcacgcattc
     3361 agcaaatgtg gcggtaacct cggcactgac ggttccgtag cttatctgtt cagcaaaaaa
     3421 ggcgtgatct ccttcgagaa aggcgacgaa gacaccatca tggaagcagc tctggaagca
     3481 ggtgctgaag acgttgtgac atatgatgac ggcgcgattg atgtctacac tgcatgggaa
     3541 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     3601 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcgcc gaaactgatg
     3661 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtttacca taacggtgaa
     3721 atctctgatg aggttgcagc gactctctga tgatgcctgc taaacagcaa aacggagacg
     3781 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgaccggct acggcgtcat
     3841 ccgccaggta ggtaggcaac tgtcttacct gggtagcgga tgcatacgca ccaaagtgga
     3901 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     3961 ccagcctgat tatttcgcca ttgaacaagt ctttatggcg aagaacgctg actcagccct
     4021 gaaactgggc caggcgcgcg gcgtggcgat tgttgcggcg gtgaatcagg aattgccggt
     4081 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtatgggta gtgccgaaaa
     4141 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     4201 tgccgccgat gcgctggcga ttgccatcac ccactgccac gttagtcaga atgcgatgca
     4261 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     4321 gtcagttctg gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccaa acccggatga
     4381 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     4441 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttgtcc aaacgtctct
     4501 atttcaccca ccggaataca tagcgcctct ttataaatat caaagacaaa ttttg
//
LOCUS       DADLTI010000237         4498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLTI010000237 DADLTI010000000
VERSION     DADLTI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.241"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN755_003969"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN755_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7382568.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN755_003970"
     CDS             1227..1760
                     /locus_tag="JN755_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7382569.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..1986)
                     /locus_tag="JN755_003971"
                     /pseudo
     CDS             complement(1757..1986)
                     /locus_tag="JN755_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN755_003972"
     CDS             complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN755_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7382570.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN755_003973"
     CDS             complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN755_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7382571.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN755_003974"
     CDS             complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN755_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7382572.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3204..4232
                     /gene="epmB"
                     /locus_tag="JN755_003975"
     CDS             3204..4232
                     /gene="epmB"
                     /locus_tag="JN755_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7382573.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1157 a         1135 c         1119 g         1087 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacttg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagagagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcatc aaacggctaa agccgtgggt
     1921 atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca
     1981 ggacatatca ggctccggaa atttggggcc gtccccggtg aaaagaggcg tttgcaggtc
     2041 gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat
     2101 tacctttatg acgatcgcag aaaaaacaaa cggcacgaca ctgctgtcgt accgttattg
     2161 cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt
     2221 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
     2281 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
     2341 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat ctgatgattt
     2401 ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga gatgtttgat
     2461 gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
     2521 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
     2581 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
     2641 gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag tagacagggt
     2701 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
     2761 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
     2821 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcatcagc
     2881 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
     2941 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
     3001 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
     3061 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
     3121 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
     3181 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
     3241 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
     3301 tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc
     3361 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatcctcttt
     3421 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc tccaccgacc
     3481 cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
     3541 ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc
     3601 cctatgccga aaatcagggc aacaagcgta actggcagac tgcacttgag tatgttgctg
     3661 cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
     3721 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
     3781 ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct
     3841 ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc aatgaggtag
     3901 atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc
     3961 agagcgttct gttacgtggt gtgaacgata acgcacaaac gttggcaaac ctgagtaatg
     4021 cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctagataaa gtacagggcg
     4081 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacat
     4141 tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa
     4201 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt taaacattaa
     4261 aattgcctga tgcgctacgc ttatcaggcc tacatcgtgc attgaaattg aatggatttg
     4321 caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa
     4381 gtacagcaac ctgtcattaa actgagaaat ttaatctttg cacgttgcat aacctttaat
     4441 tattcatatg cattgctgtt gttacttttt atatagctaa caccaataat taaccttt
//
LOCUS       DADLTI010000238         4455 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLTI010000238 DADLTI010000000
VERSION     DADLTI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.242"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..253
                     /locus_tag="JN755_003976"
                     /pseudo
     CDS             1..253
                     /locus_tag="JN755_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JN755_003977"
     CDS             746..1492
                     /locus_tag="JN755_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7382574.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JN755_003978"
     CDS             1507..1929
                     /locus_tag="JN755_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7382575.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JN755_003979"
     CDS             1987..2343
                     /locus_tag="JN755_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382576.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JN755_003980"
     CDS             2436..2654
                     /locus_tag="JN755_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY7382577.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JN755_003981"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JN755_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3683
                     /locus_tag="JN755_003982"
     CDS             3018..3683
                     /locus_tag="JN755_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382578.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="JN755_003983"
     CDS             3683..4048
                     /locus_tag="JN755_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7382579.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1189 a          948 c         1264 g         1054 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaattc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcaca
//
LOCUS       DADLTI010000239         4392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLTI010000239 DADLTI010000000
VERSION     DADLTI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.243"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..876)
                     /gene="adiC"
                     /locus_tag="JN755_003984"
     CDS             complement(<1..876)
                     /gene="adiC"
                     /locus_tag="JN755_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7382580.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSSVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGG"
     gene            complement(1013..1774)
                     /locus_tag="JN755_003985"
     CDS             complement(1013..1774)
                     /locus_tag="JN755_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7382581.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2099..4366)
                     /gene="adiA"
                     /locus_tag="JN755_003986"
     CDS             complement(2099..4366)
                     /gene="adiA"
                     /locus_tag="JN755_003986"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7382582.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1133 a         1049 c         1119 g         1091 t
ORIGIN      
        1 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccagcggt
       61 gtcacccaac gccatccgtg cggcatcacc gaatggcgag gcagaaacgc gcagtgcggc
      121 attagggatc atccccataa tcgcggtggt ggagagcaca tagcaaacgg cggcaatcaa
      181 taccccacca atggtggcga tagggacatt gcgtttcggg tttttcacca cacctgcggc
      241 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
      301 cgcaccgaag gtgcccaggc cgctgacgtt ccatgccgcc atataggttt caccacggaa
      361 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cagtggcaac
      421 ggcctgcacg cgggtgatca ttttcggacc gacaatgttc agcaggacga agatccacag
      481 caccacgacg caggtgatgg ttaataccaa tggatctttc agaatcggga agaagtaact
      541 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
      601 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
      661 accaccagga cttgggtcga ggaacgacat tttggcgtat accatcgaga gccccagcgc
      721 accgataatc gtcaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
      781 aggtaacaga aaaacacttg accccataat attccccgac accatcaggg tgacggggat
      841 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
      901 ttcgtgacaa taaattacgt catatcatac gcctgcattc gatagttgaa ttattatcga
      961 ccgggttatc gctgatgccg ttattaatat agctcttcgt taaggttaaa aatcaggcga
     1021 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
     1081 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
     1141 ttttaccgtc catcattaat tcatttacgg cataacgcat gcggcaggtg gtgattattt
     1201 ggctataact ggtgttttcg cttttcaact ttttctttaa cagacttggg ctaagacata
     1261 aacaactggc taccatacta agattccagt ctttgtgaat atcgctttca ataatttgat
     1321 aaacgctgtc acttacacaa ttacgtaaca tatacatcat taatgaaagg aattttttac
     1381 tgtcgagaaa acgggatagc accgtgaata ataatgcgcg tgttcgttcc ttttctgact
     1441 cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg aaagacgtct ggcgtcaggc
     1501 atggcagggt gagtatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
     1561 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
     1621 tgttaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atatggttag
     1681 ctttaaggct aataggttct ttcccattca cccttatccg gacatctttt tcagtcaata
     1741 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
     1801 gaccagttct cgctaaagca aagcgatacc gggaaatggt tatataatta tatgatgaat
     1861 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
     1921 atgtacatat ttttttacag gataaataac aggcaaaaaa aagcgcgggg tggggccgcg
     1981 caaaaagtat gacatttttg tttgcagcaa tttttatgaa aacgttgcca tactcaaaag
     2041 gcctaaggcc aggcggcgta atgttattta aacaattacg ccttcagcgg aatagtggtt
     2101 acgctttcac gcacataacg tggtaaatac cgtcaataat ttcagtccct tcagtttcgt
     2161 gttcaaatcc agggaaatgg tggtcccagg attgcagcga gcgtaaataa cttacttgcg
     2221 gactgttttt atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatacg
     2281 ggataactga gtttgccgcg atgcgtcctg gcagattttc aatggatacc agttcgacat
     2341 tgttgtcgac aatcgcgttg tacgcttcac gcggggtgat ttccgccacc ggcaggccgg
     2401 aataggcttc gttcaaccgt gcgccagggt tgttttcttt cagccaggca aacatggtgt
     2461 cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg
     2521 gcatcacctg cgccagcggt gtgttggcgt catagtggcg tttgaaggag caaagggtgt
     2581 taaccagagt cccccatttc ccacgggtta cgcccataga gaacaggaac ataatttgga
     2641 agtcagtggt gcgggtaggc acaatgccgt ggcgaccaag ccaggcagtg accagcgccg
     2701 ccggaacacc ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga
     2761 ctttaattgg gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt
     2821 cgcccggatg tattacccag cagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag
     2881 caaagtcata ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga
     2941 agaaccagct accgtcagcg gtgaactctt tatatagccg cgccatcgcc tgacggaaat
     3001 caaccgcttc gttaatcact tcctgtgtca gtgacaggcc gctgttgccg tccatcatcg
     3061 acaccgccac gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca
     3121 tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat
     3181 aagaagcctg tgacagcgca ttcagcagtt tgtggatgga gtgggtggcg aaaacggtag
     3241 gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga
     3301 aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca
     3361 gatcctgcgc ttctttagcg ttataacaca cgccgtcata ggtgcagttg gttaccacgc
     3421 agtaagacgg tttttgcccg gctttgtctt tggtcagcgg gctttcactg attttctttt
     3481 gcaaggtttc aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc
     3541 ggcttggcac catatagacc ggtttcgcgc ctgtcagcat caaaccttgt tcgatggatt
     3601 tatggcagtt acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg
     3661 tgcggttaga gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc
     3721 gtgcggcata tttttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg
     3781 aagttcgttc gatgcccatg tcggtgcgga acagattttc accatagtag tcatggtaga
     3841 aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg
     3901 aatattcatg gatgtcacta tatttcatca gcgcgctgaa cagtggcggc aacagctgct
     3961 ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggcg gtatcttcca
     4021 gaatccaggc gaattcatcg acaagctcca gcagatcccg gtccattgcg gcgagggctt
     4081 tttcccgatc gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga
     4141 tcaattgtct gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc
     4201 agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa
     4261 tcacggtaac attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc
     4321 aggtgtcttg atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg
     4381 ttatgaagga at
//
LOCUS       DADLTI010000240         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLTI010000240 DADLTI010000000
VERSION     DADLTI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.244"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(64..822)
                     /locus_tag="JN755_003987"
     CDS             complement(64..822)
                     /locus_tag="JN755_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7382583.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JN755_003988"
     CDS             complement(830..1933)
                     /locus_tag="JN755_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7382584.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JN755_003989"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JN755_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JN755_003990"
     CDS             complement(3181..4206)
                     /locus_tag="JN755_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7382585.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1214 a         1147 c         1023 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADLTI010000241         4326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLTI010000241 DADLTI010000000
VERSION     DADLTI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.245"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..157)
                     /locus_tag="JN755_003991"
     CDS             complement(<1..157)
                     /locus_tag="JN755_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382586.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JN755_003992"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JN755_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7382587.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JN755_003993"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JN755_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7382588.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JN755_003994"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JN755_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7382589.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4326)
                     /locus_tag="JN755_003995"
     CDS             complement(3800..>4326)
                     /locus_tag="JN755_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7382590.1"
                     /translation="TTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1144 a         1083 c          992 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacaacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc atgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgc
//
LOCUS       DADLTI010000242         4321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLTI010000242 DADLTI010000000
VERSION     DADLTI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.246"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..756
                     /gene="yegD"
                     /locus_tag="JN755_003996"
     CDS             <1..756
                     /gene="yegD"
                     /locus_tag="JN755_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7382591.1"
                     /translation="DYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLL
                     GHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGIALPILPWWNAVAINDVPAQSDFY
                     SSANGRLLNDLVRDAREPEKVALLQKVWRQRLSYRLVRSAEECKIALSSVAETRASLP
                     FISNELATLISQRGLESALSQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKK
                     ALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(768..989)
                     /locus_tag="JN755_003997"
     CDS             complement(768..989)
                     /locus_tag="JN755_003997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7382592.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(1185..2708)
                     /locus_tag="JN755_003998"
     CDS             complement(1185..2708)
                     /locus_tag="JN755_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY7382593.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(2705..3466)
                     /locus_tag="JN755_003999"
     CDS             complement(2705..3466)
                     /locus_tag="JN755_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7382594.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3463..4122)
                     /locus_tag="JN755_004000"
     CDS             complement(3463..4122)
                     /locus_tag="JN755_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7382595.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         1106 a         1166 c         1085 g          964 t
ORIGIN      
        1 gattacgaag ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt
       61 acgactgact gttcattgct gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa
      121 gccagtctgc tgggtcacag tggttgtcgt attggcggta acgatctgga tatcgcccta
      181 gcgtttaaaa acctgatgcc actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc
      241 ctgccgatcc tgccgtggtg gaatgcggtt gccatcaacg acgttcctgc acagagtgat
      301 ttctacagta gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg
      361 gaaaaagtgg ccctgttaca gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt
      421 agcgcagaag agtgcaaaat tgctctttca agcgtagcgg aaacccgcgc ctcactgccg
      481 tttatcagca atgaactggc tacgctgatt agccagcgag ggctggaaag cgccctcagc
      541 cagccactgg cgcggattct ggaacaggtg caactggccc tggataacgc ccaggaaaaa
      601 ccggacgtta tctacctgac cggcggtagc gcccgttctc cgctgattaa aaaagcgctg
      661 gcagaacagt tgccgggcat tccgattgca ggcggcgatg actttggctc cgtcaccgcc
      721 gggctggcac gctgggcgga agtggtattt cgttaaaaga aaatggctca gcagcggctt
      781 aaatcagcct gcgcctgagc cagtttttct gccgcctgac gtaacggttc catcaacggc
      841 atggtccgtg ctggagcatg aaggcggaat cgctgcaact ctgtcgcgcc gacagtcaac
      901 gtcgattcca gccgatgtcg tttggcggcc attttcgcca ttaccgcctg tctgtctgct
      961 ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt cacagctcgc tttccagtcg
     1021 atgaccgcct gggtcagatg atcaccaaac cctttaactt gcttaacgcc acgacgggta
     1081 acacctgctg ctgtttcaat accaaaagac cataacgccg ctttacgcgc agggccaacg
     1141 ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctactt ctgccgttcc
     1201 cttgcagtgt catgaagtgc tgccagagcg agtttctctt cttccggtaa cccgagaatt
     1261 tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag gccgcctacc
     1321 tgctggatct ggcggactaa atggtcatag tccattttcg cacgattaaa tgcctcacgg
     1381 cgttgctgaa cttctgcttt gtacgctttg cttgtcagac tgccaataat ccagatagcc
     1441 gccagcacag gaaccaaaat aatataacgc ggttctgcct gaaggccgca aagcagcagc
     1501 gataatgctg acagtgcgat ctcaagcaga atgatgtatt cgcgccgtaa caggcctaaa
     1561 ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gcagttgcaa tgctggcggt
     1621 gctactgacg ccatcaccat cgcccagact tttgccagca caaaatcccc gccggtggta
     1681 atgacctctt cgccgagatc aataaaatag ataacgcctt gattatccag cgtacaccac
     1741 aggcagtcgg tcaaatgagc gggataaaca tgcattgccg aaacgggaca tttctttaac
     1801 tgttggcgca gagaatcaag cgccgctacc cacgctttag ccgtcggacg cccggttgcc
     1861 acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catcactcgg taacatcgat
     1921 agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct gattatctga cgcgtaggca
     1981 taacggaaat gggtaatatc cgtctccagc gcattacccg catcagagat aagcggcaca
     2041 ccagaataag gatgccggcc accaaacaag acgtgaaaaa tcagcaacgc aaggccaaaa
     2101 ttaccgtgat tttctgtgcg ttcaaagccg acaaatgacg acaaggtttg tagctctggc
     2161 ggcgtaaaat gcgacacgcc gacttcgcat aaatgcagtg tgccattggc gttaatctga
     2221 aaggagtcgc tatcaatcaa caccactttg ctgtcgcgac ctaccataaa actgttctgg
     2281 tttacgtcac ccacgacatg cccgtgctcg tgaaccgtag caaaagatga agcaatattg
     2341 cgcgcaacat agagtagaaa atcccacgca caatgagggt aacgttgacg acgatgtgcc
     2401 gggctataga tcatatgaat cggttcttta ccagaaactt ttggcatcat aaaaccgata
     2461 acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata attcaacaat
     2521 tgcgcgtcag ctgtcgcagc cataaaggca agtttgtcct gttttaaggc gggtggcggc
     2581 gtgtgataaa tcttggcgac gctatcgaca aactcattga tatcataaac cgcgccttca
     2641 ccgcctttgc ccagttcacg gcctggcgta acgcactcgc cagtagcagt atataaagtg
     2701 ggtttcatgg taaccacagc gccagtgcca acgttttatc atcatccgta cgctcgttta
     2761 ccgccgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt tgttcctgcg
     2821 ttgctgacgc cagtccattg aaaaacgggg taaagaaagg cacatgcgga gaattatcca
     2881 acatatttaa cgccagtcgc tggataccat ccgtaaatgc tgcaacttta tgcacacgct
     2941 cagtgctggt gaaagtttcc agtctggaaa cggcatcttc atcggtaatg aagtgcgtca
     3001 tattggcata ttcacccacc atcggcgtaa gcggcaattg cagaccgtga ccaaaatcca
     3061 ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc attagccgat gaaatcaatc
     3121 ccagaaacgt gcaggcaaaa tcgcgaactg ctaattcttt ggcttccgcc tcggcaaaaa
     3181 gtcgctgacg aacggtcagc acaatatccg tggcgaggat atcattaagc cccaattccc
     3241 caccctgcac tttttgcgac atataagcca tcgcttcatt gacggcgagc atcgcgcctt
     3301 cgcccccctg cgaaacgcta cctgcgccat cagcaaggaa catcaccagt aatggctgct
     3361 ggtcatttag ccaggcaacc tgcatctggc aggcatcctg gcaaggcaag tccgcgctga
     3421 tgtgcgacgt tccaacagcg gatgcataaa ccagacgcca gctcacactg acgtccagcc
     3481 ttttggcgct tccagcacaa cttccgttcc cggcgtggag cgggaaaccg aacgcagcga
     3541 actggataac cagctaaaca gttcacggaa ttgtaatcct tgcagaggca aaggctgacg
     3601 aacgctgatt tgtgccagag tcttcatatc cgccccctga acaccgatgg aaaagaaagc
     3661 aaacctttta tcttcttccc cccgaaacac tttgttggct gcagcctgcc actcatcggt
     3721 tggtgcacca tcggtaatca ggaaaatcca cgggcgataa tacgaaatac catttgcacg
     3781 atactcacgt ttccgctcct ctaccatatc cagggctttg gtaatggccg cacccattgg
     3841 tgtatcgccc tgggcaaaca ggatgggcgg aaaaaaatta gcggcgctgg taaagggttg
     3901 ttccacatgc accgggccga acgtcacaat gccgagttcc actcttttta aagccagcga
     3961 atcggcaagc agctcatcgc gaaaggtaac caatccggtg ttaagttcat taataggtct
     4021 gccactcatc gagccagaaa catccagcag cagaatgcag gggcaacgtg gttccgggtt
     4081 actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc ctttcttaga
     4141 gaattattga ggtccggtat cataccgact gcaataatga aataagagca aacgaagaaa
     4201 ctttcagaat tagtcccggc ggcgcagaaa attttgctat tcgcctcgca agaacggaga
     4261 gccgtcgatt gacactaagg gcggagtgac ataatttcag gagtgagggt tagggagagg
     4321 t
//
LOCUS       DADLTI010000243         4273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLTI010000243 DADLTI010000000
VERSION     DADLTI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.247"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..158)
                     /locus_tag="JN755_004001"
     CDS             complement(<1..158)
                     /locus_tag="JN755_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7382596.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JN755_004002"
     CDS             complement(308..469)
                     /locus_tag="JN755_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382597.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JN755_004003"
     CDS             complement(466..669)
                     /locus_tag="JN755_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7382598.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JN755_004004"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JN755_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7382599.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JN755_004005"
     CDS             complement(1530..1661)
                     /locus_tag="JN755_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7382600.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2161..2237)
                     /locus_tag="JN755_004006"
     tRNA            complement(2161..2237)
                     /locus_tag="JN755_004006"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JN755_004007"
     tRNA            complement(2339..2414)
                     /locus_tag="JN755_004007"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="JN755_004008"
     CDS             complement(2558..3379)
                     /locus_tag="JN755_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7382601.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="JN755_004009"
     CDS             complement(3394..3750)
                     /locus_tag="JN755_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7382602.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4273)
                     /locus_tag="JN755_004010"
     CDS             complement(3763..>4273)
                     /locus_tag="JN755_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7382603.1"
                     /translation="EAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAP
                     LPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPCACCGKP
                     ADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLD
                     HAFATGVLG"
BASE COUNT         1176 a         1158 c          841 g         1098 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaacc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccactatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tcc
//
LOCUS       DADLTI010000244         4273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLTI010000244 DADLTI010000000
VERSION     DADLTI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.248"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            158..827
                     /locus_tag="JN755_004011"
                     /pseudo
     CDS             158..827
                     /locus_tag="JN755_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1765)
                     /locus_tag="JN755_004012"
     CDS             complement(1070..1765)
                     /locus_tag="JN755_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7382604.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1758..3185)
                     /locus_tag="JN755_004013"
     CDS             complement(1758..3185)
                     /locus_tag="JN755_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7382605.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3196..3915)
                     /locus_tag="JN755_004014"
     CDS             complement(3196..3915)
                     /locus_tag="JN755_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7382606.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1147 a         1100 c          879 g         1147 t
ORIGIN      
        1 ccagatatgg ggaccgaatc gttatataaa acactgaggt aactctcatg cttcatatcc
       61 agtattcatg atgctggttt tttgctgata tttaacccga acattcctga tacagactat
      121 taatacaaca ttatcttcga cagattttag atatattatg agtgaacaaa tcaaacagga
      181 tatcgatctg attgagattt tatttcatct gaagaaaaag attcgtgtta tcctttttat
      241 tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag acaatacaaa
      301 agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta gctgtgatag
      361 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      421 attttttcag accagcccag atatcaagaa cagagaaata aggctggaat ggtcaggaga
      481 taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa
      541 atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      601 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      661 tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata aataccctgc
      721 tgccatttgc ctagctctgg gatttttttc taagcattgt aatttctgta atgttttgcc
      781 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
      841 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
      901 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tactaatgcc tggatattta
      961 cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     1021 tcaacctcgt atgccttctt caggtttatg ttcagatctc atatctctct cagcaatcct
     1081 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     1141 tatccgccgt tgagctggga ccactgatga tgttaataca tgacggcatt cgttcaccgg
     1201 cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt
     1261 tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg
     1321 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     1381 ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg
     1441 cattgcattc ctgctgcaaa cgttcactaa tccccaattc ctccagcctc gtgtcaccgc
     1501 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     1561 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     1621 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttgttaagcg
     1681 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     1741 tcaaaaattc gctccgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     1801 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     1861 gcgccaaatt tgagtggtgt tttaccgcca ttgataaacc agcttgccgc atgagcgccg
     1921 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     1981 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     2041 atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcagt gcataaagag
     2101 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     2161 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     2221 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     2281 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     2341 tgccccgctt cgcgcggccc tgtcagccag gtattgtatc ccgtcaaacg tgcaccaacg
     2401 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     2461 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     2521 tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca gccggtaata
     2581 cctatttcag cactgaggaa atcttcacgg attttttgcc ggatgaataa ggtcatcgct
     2641 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     2701 cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga
     2761 atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     2821 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     2881 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     2941 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaaa
     3001 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc caattcatcg
     3061 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     3121 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     3181 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     3241 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     3301 taaatactca gggcggactt ccatcaggtg cacaaccttt tctttcacca tctcgccgga
     3361 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     3421 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     3481 tcccagctta cgggccaggc tacaagatgg gtgatacaca gctctccctt gcaaactggc
     3541 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     3601 ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     3661 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     3721 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc tattgatcgc
     3781 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     3841 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     3901 gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc cgatgtttcc
     3961 cccccaccgg gggatcaatc atcaataaat atggcgttct ttttaaattg gctagcaaca
     4021 taccacccct catttgtatg acaaatgata atctttcatc caaacttcaa atgcaataac
     4081 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc
     4141 ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct acttattcag
     4201 gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa tcacgtatca
     4261 agtaatagtc gga
//
LOCUS       DADLTI010000245         4263 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLTI010000245 DADLTI010000000
VERSION     DADLTI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.249"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..826
                     /locus_tag="JN755_004015"
     CDS             <1..826
                     /locus_tag="JN755_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7382607.1"
                     /translation="TQLLNFDPSTMQWATSSPVPSTFIGALIISIVAGYLVKWMNQKI
                     QLPDFLLAFKTTFLLPILSAIFVMLAMYYVITPFGGWINGGIRTVLTAAGEKGALMYA
                     MGIAAATAIDLGGPINKAAGFVAFSFTTDHVLPVTARSIAIVIPPIGLGLATIIDRRL
                     TGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFALESPITAIPSYMVGAIVGSTAAVWL
                     GAVQWFPESAIWAWPLVTNLGVYMAGIALGAIITALMVVFLRLMMFRKGKLLIDSL"
     gene            841..1924
                     /gene="ypdF"
                     /locus_tag="JN755_004016"
                     /pseudo
     CDS             841..1924
                     /gene="ypdF"
                     /locus_tag="JN755_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            1924..2961
                     /gene="ypdE"
                     /locus_tag="JN755_004017"
     CDS             1924..2961
                     /gene="ypdE"
                     /locus_tag="JN755_004017"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7382608.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            2986..>4263
                     /locus_tag="JN755_004018"
     CDS             2986..>4263
                     /locus_tag="JN755_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7382609.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE"
BASE COUNT          913 a         1138 c         1259 g          953 t
ORIGIN      
        1 gacgcaactg ctgaacttcg atcccagcac tatgcaatgg gcgacctctt cgccggtgcc
       61 gtccaccttc attggtgcgc tgattatttc tatcgttgcc ggttaccttg tgaaatggat
      121 gaaccagaaa atccagttac cggatttcct gttagcgttc aaaaccacat ttttgctacc
      181 gattctttcc gccatttttg tcatgctggc gatgtactac gtcatcaccc cctttggtgg
      241 ctggatcaac ggcggtatcc gtactgtgct gactgccgca ggtgagaagg gcgcgctgat
      301 gtacgcaatg gggatcgccg cggcgacagc aatcgacctt ggtggcccga tcaacaaagc
      361 cgcaggtttc gttgccttca gctttaccac tgaccacgta ttaccggtca ccgctcgttc
      421 tatcgctatc gttattccgc cgattggtct gggtctggcg accattattg accgtcgttt
      481 aaccggcaaa cgcctgttca acgctcaact ttatccgcag ggtaagaccg cgatgttcct
      541 ggcctttatg gggattagtg agggtgcgat tccgtttgcg ctggaaagcc ccatcaccgc
      601 cattccgtca tatatggtcg gcgcgattgt cggctcaacc gcagctgtct ggctgggcgc
      661 agtgcaatgg ttcccggaat ccgctatctg ggcatggccg ctggtcacta acctgggcgt
      721 ttatatggcg gggatcgcgc tgggggcaat cattactgcg ctgatggtgg tgttcctgcg
      781 tctgatgatg ttccgcaaag gcaaattgtt aatcgatagc ctgtaagaag gaaaatccag
      841 atgacattac tcgcttcgct gcgcaactgg cttaaggtgc aacaactgga tgcggtgctt
      901 ctctcctcac ggcagaacaa acagccgcat ctggggatct ccaccggatc aggctatgtg
      961 gttattagcc gtgaaagtgc gcacattctg gtagattcgc gctatttcgt tgaggtggaa
     1021 gcccgtgcgc aaggctacca gctgcatttg cttgacgcga cgaacacgct taccactatc
     1081 gtcaatcaaa tcattgccga tgagcagttg caaacgctcg gttttgaagg ccagcaggtg
     1141 agttgggaaa ccgcgcaccg ctggaagtct gaactcaatg cgaaactggt tagcgccacg
     1201 ccggatgtgc tgcggcaaat caaaacgcca gaggaggtgg agataatccg cctcgcctgt
     1261 gggattgccg atcgcggagc ggagcatatt cgccgcttta ttcaggcggg gatgagcgag
     1321 cgcgagatag ccgctgaact ggagtggttt atgcgccagc agggcgcaga aaaagcctct
     1381 tttgacacca ttgtcgccag cggctggcgc ggggcgctgc cgcacggcaa agccagcgac
     1441 aagattgttg cagcgggcga gtttgtcact ctcgattttg gtgcgctgta tcagggctac
     1501 tgctgatatg acgcgcacct tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc
     1561 gctgtttgac gtctatcaga ttgtcctgca ggcacagctc gcggcaatct ctgcaattcg
     1621 ccccggcgtg cgctgccagc agattgacga cgccgcgcgc cgggtcatta ccgagtctgg
     1681 atttggcgac tatttcggtc ataacactgg tcacgctatc ggcattgaag tccatgaaga
     1741 tccgcgtttt tcaccgcggg acaccacgac gctacagcca ggcatgttgc tgaccgtgga
     1801 gccggggatt tatttgctag ggcaaggggg cgtgcgcatc gaagatgttg tgctggttac
     1861 cccgcaaggc gcagaagtgc tctacgccat gccgaaaaca gtgttgctca cgggagaggc
     1921 ataatggatt tatcgctatt aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa
     1981 caggaagtgc ggcagatcct gctggaagaa gcggctcgcc tgcaaaaaga agtgcgattt
     2041 gatggtctgg gatcggtgct gatccgcctc aatgaatcga caggtccgaa ggtgatgatc
     2101 tgcgcccata tggacgaagt gggatttatg gtgcgcagca tctcccgcga aggcgctatt
     2161 gacgtgctgc cggttggcaa cgtgcgcatg gctgcccgcc agctgcagcc ggtgcgcatc
     2221 accacccgtg aagagtgcaa aattctaggc ctgcttgacg gcgaccggca ggggaatgac
     2281 gttagcgcca tgcgcgtgga cattggcgcg cgcacctatg acgaagtgat gcaggcggga
     2341 attcgtccag gcgatcgcgt caccttcgat accacttttc aggtcctccc tcaccagcgg
     2401 gtgatgggca aagcctttga tgatcgcctc ggttgctatc tgctggtgac gttactacgt
     2461 gagctgcacg acgccgaact gcctgcggaa gtgtggctgg tggcaagttc cagcgaagag
     2521 gtgggattac gcggcgggca aactgctacc cgcgcggtgt cgccggacgt cgccattgtg
     2581 cttgataccg cctgctgggc gaaaaacttt gattatggcg cggctaacca tcgtcagatt
     2641 ggtaacgggc cgatgctggt gttaagcgat aaatcgctga ttgcgccgcc aaaacttacc
     2701 gcgtggattg aaaccgtggc ggcagaaatt ggcgttccgt tgcaggcgga tatgttcagt
     2761 aacggcggca cggacggtgg agcggtgcac ttaaccggta ctggcgtacc cacattggta
     2821 atggggcctg ccacccgcca cggacattgc gcggcatcga ttgccgattg ccgcgacatt
     2881 ttgcagatgg agcaactttt atctgccctt attcaacgtc ttacgcgtga gacggttgtt
     2941 caactgacgg atttcagatg atctcctgat taacccggag cggttatgtt aacgattcaa
     3001 tttctctgtc ctttgcctat gcctaacggt ctacatgctc gtccggcgcg ggaacttaaa
     3061 gaacagtgca gccagtggca aagcgaaatc acctttatca accatcgcca gaacgcaaag
     3121 gccgatgcta aaagctcgct ggcgttgatt ggaactggca ccttatttaa tgacagttgc
     3181 agcctgaaca ttagcggcag cgatgaagag caggcgcggc gcgtgctgga agagtacatc
     3241 ctggtgcgct ttatcgacag cgacagcgtt cagccgacgc aggcagaact gacggcgcat
     3301 ccgctgccgc gttcattaag ccgccttaac ccggatttac tgtacggcaa tgtgctggca
     3361 agcggcgtcg gcgtgggtac gctgaccctg ttacaaagcg acagcctcga cagttatcgg
     3421 gcaatccccg ccagtgcgca agattccacc cggctggagc acagcctggc aacgcttgcc
     3481 gagcaactga atcagcaatt gcgtgagcgt gacggcgaaa gcaaaactat cctcagcgct
     3541 catttgtcgc tgattcagga tgatgaattt gcaggcaata tccgtcgcct gatgacagaa
     3601 cagcatcagg gactgggggc ggcgatcatc agcaatatgg agcaggtttg cgccaaacta
     3661 tctgcttctg ccagcgatta tctgcgcgag cgggtcagcg acattcgcga tatcagcgaa
     3721 cagttgctgc atatcacctg gccggaactg aagccgcgca acaatctggt gctggaaaaa
     3781 ccgaccattc tggtggctga agatttaacc ccaagccagt ttttgagcct tgatttgaaa
     3841 aatcttgcgg gtatgattct ggagaaaacc gggcgcacct cgcatacact gattctggcc
     3901 cgtgcctcgg cgatcccggt actgagtggc ttgccgctgg atgcgattgc ccgttatgcc
     3961 gggcaacctg cagtgcttga cgcccagtgc ggcgtgctgg cgattaaccc gaatgacgcg
     4021 gtgagcggtt attatcaggt cgcgcagacg ctggcggata aacgccaaaa acaacaggcg
     4081 caggctgccg cgcagctggc ctattcccgt gataacaagc gtattgatat tgcggcgaat
     4141 atcggcaccg ctctggaagc gccaggcgcg tttgccaacg gcgcggaagg tgtcgggctg
     4201 ttccgtaccg aaatgctcta tatggatcgc gacagcgcgc cggacgagca ggagcaattt
     4261 gaa
//
LOCUS       DADLTI010000246         4238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLTI010000246 DADLTI010000000
VERSION     DADLTI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.250"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..231)
                     /locus_tag="JN755_004019"
     CDS             complement(<1..231)
                     /locus_tag="JN755_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7382610.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHY"
     gene            complement(346..421)
                     /locus_tag="JN755_004020"
     tRNA            complement(346..421)
                     /locus_tag="JN755_004020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Thr,seq:ggt)
     gene            complement(428..502)
                     /locus_tag="JN755_004021"
     tRNA            complement(428..502)
                     /locus_tag="JN755_004021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(467..469),aa:Gly,seq:tcc)
     gene            complement(619..703)
                     /locus_tag="JN755_004022"
     tRNA            complement(619..703)
                     /locus_tag="JN755_004022"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(667..669),aa:Tyr,seq:gta)
     gene            complement(712..787)
                     /locus_tag="JN755_004023"
     tRNA            complement(712..787)
                     /locus_tag="JN755_004023"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Thr,seq:tgt)
     gene            1149..2099
                     /gene="coaA"
                     /locus_tag="JN755_004024"
                     /gene_synonym="panK"
     CDS             1149..2099
                     /gene="coaA"
                     /locus_tag="JN755_004024"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7382611.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2128..3093)
                     /gene="birA"
                     /locus_tag="JN755_004025"
     CDS             complement(2128..3093)
                     /gene="birA"
                     /locus_tag="JN755_004025"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7382612.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3090..4118)
                     /gene="murB"
                     /locus_tag="JN755_004026"
     CDS             complement(3090..4118)
                     /gene="murB"
                     /locus_tag="JN755_004026"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7382613.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1097 a         1050 c          947 g         1144 t
ORIGIN      
        1 gtagtgacgg gtcggggtgt catattcaac gtgagaagtg ttgatggtga taccacgagc
       61 tttttcttcc ggcgcgttat cgatctggtc gaatgcgcga gcagcaccgc cgtaggtttt
      121 agccagtacg gtagtgattg cagcggtcag cgttgtttta ccatggtcaa cgtggccgat
      181 agtaccgacg ttaacgtgcg gttttgtacg ttcaaacttt tctttagaca tcgattgtcc
      241 ctctaagaca cggataaatc ggtgatatca ccacatcaac caggcaacat gcccgacttg
      301 ttgaatgcaa taaacagaag gaaaaaacag ggaggagaaa agaagtggtg ctgataggca
      361 gattcgaact gccgacctca cccttaccaa gggtacgctc taccaactga gctatatcag
      421 cacatcttgg agcgggcagc gggaatcgaa cccgcatcat cagcttggaa ggctgaggta
      481 atagccatta tacgatgccc gcatcctaga actcggctac ctgattttca ttctgcactg
      541 aatatcgaga gaagctctct ttattcgagc cggtaagtga acttatcgtc tcgggctacg
      601 ccatcgcgtg gccgaaattg gtggtggggg aaggattcga accttcgaag tctgtgacgg
      661 cagatttaca gtctgctccc tttggccgct cgggaacccc accggacttg atggtgccga
      721 ctaccggaat cgaactggtg acctactgat tacaagtcag ttgctctacc tactgagcta
      781 agtcggcatc aagtagcgcg cattctatgg agacatgcga gttcatgcaa ctaaaaaatt
      841 gcataatttg ttttattggt cacattttat gctacacgat gaagaaacag ccgaacggag
      901 ggcagattag cacacttttt caacatcatt gtgctcaaca atgcgctcct gctaaaccat
      961 aattcttttt atcagatgga atatctgtca cattgctttt caacgatagc ttcctggcag
     1021 agattttttc ttattattcc tccccatctg gtgttaccct cctgcccatt aacccattca
     1081 acagaactgt gacgcgccat ggcaaatatc gctttgccga tagagctatg accgccagaa
     1141 acatgcttat gagtataaaa gagcaaacgt taatgacgcc ttacctacag tttgaccgca
     1201 accagtgggc agctctgcgt gattccgtac ctatgacgtt atcggaagat gagatcgccc
     1261 gtctcaaagg tattaatgaa gatctctcgt tagaagaagt tgccgagatc tatttacctt
     1321 tgtcacgttt gctgaacttc tatataagct cgaatctgcg ccgtcaggca gttctggaac
     1381 agtttcttgg taccaacggg caacgcattc cttacattat cagtattgct ggcagtgtcg
     1441 cggtggggaa aagtacaacc gcccgtgtat tgcaggcgct attaagccgt tggccggaac
     1501 atcgtcgtgt tgaactgatc actacagatg gcttccttca ccctaatcag gttctgaaag
     1561 aacgtggtct gatgaagaag aaaggcttcc cggaatcgta tgatatgcat cgcctggtga
     1621 agtttgtttc cgatctcaaa tccggcgtgc caaacgttac agcccctgtt tactcgcatc
     1681 ttatttatga tgtgatcccg gatggagata aaactgttgt tcagcctgat attttaattc
     1741 ttgaagggtt aaatgtctta cagagcggga tggattatcc acacgatcca catcatgtat
     1801 ttgtttccga ttttgtcgat ttttcgatat atgttgatgc accggaagac ttacttcaga
     1861 cgtggtatat caaccgtttt ctgaaattcc gcgaaggggc ttttacagac ccggattcct
     1921 attttcataa ctacgcgaaa ttaactaaag aagaagcgat taagactgcc atgacattgt
     1981 ggaaagagat caactggctg aacttaaagc aaaatattct acctactcgt gagcgcgcca
     2041 gtttaatcct gacgaaaagt gctaatcatg tggtagaaga ggtcagacta cgcaaataat
     2101 ttgcagggga gcgaatactc ccctttctta tttttctgca ctacgcaggg atatttcacc
     2161 gcccatccag ggttttatta ttccatcctg ctcaagtaat aaagccccct gtttgtctat
     2221 tccgcgtgaa atgccaaata tttctttatc accaatgata agtttcactg ggcgattaat
     2281 aaaattatcc agcttttccc agcgcgacag ataaggtgcc aatccttctt gttcgaagag
     2341 ttccaacgca gcacgtaatt cacgtattag catggccgcc aacgtattac gatcaagatt
     2401 gatccccgct tcctgcagcg tgatccaccc ctgattaacg acactctctt caacacggcg
     2461 cattgccatg ttgatcccgg atccaatgac tatttgcgcc gcatcgccag ttttgccagt
     2521 cagctccaca agaatgcctg ccagcttgcg atcctgcaga tagaggtcat taggccattt
     2581 gacacgaact ttatctgctc ccagctttcg taatacttcc gccatcacga taccgataac
     2641 cagacttaaa ccaatcgccg ccgccgggcc ttgttccaga cgccagaaca tcgacaaata
     2701 taagtttgcg ccaaaaggcg aaaaccattt ccgcccccgg cgaccacggc cagcctgctg
     2761 gtattctgca acacaggcat cgcccgattt aagctctccg atacgatcaa gaaggaactg
     2821 attcgtggag tcaataactg gcagcacggc tacactaccg ccatccagct gacccaatat
     2881 ctgttcagca ttaagtaact ggatgggctc aggcaggctg tatcctttac ccggaacggt
     2941 aaagacatca acgccccagt cacgcagtgt ctgaatgtgt ttattaatag ccgcccggct
     3001 cattcccagc gtttcaccca actgctcgcc agagtgaaat tcaccgttcg ctaacagggc
     3061 aatcaatttc agtggcacgg tgttatcctt catgaaattg tctccactgc gctcacttca
     3121 cctgatgcac caataaagcg gacttcaggc tcaagccaga cattaaattt ttcacccact
     3181 ttttgtctta catggtgtgc cagttgcacc acatcttcgc tttttgcatt gtcttcatta
     3241 atgagaacta acgcctgttg acggtgcacc gcagccccac ccatttgcat cccttttagc
     3301 tggcactgat caataagcca acctgctgcc agttttactg aaccacccgc ctgggggtaa
     3361 tttggtgctg ttggaaattg cgccagtaat gctttagccg tttcggcaga tacaacaggg
     3421 tttttgaaga aactaccggc attgccattc acttttggat cagggagttt ggtggtgcgc
     3481 atatgacaca ccgcattaaa tacttgctgt ggcgttactg ttgtaggatc cagacgagtt
     3541 aagtcaccat acgttagtac aggttgccac tcttttggca gacgcagacc tacggctaca
     3601 atggcgaagc gatcctggta ttcatgttta aaaatactgt cgcgatagcc aaaacggcac
     3661 tctttggcag ttaagcgcac ttgcttgcct gtcgccagtt caacacaatc aacataagcg
     3721 caaactcgct gtaattctac gccataagca ccaatattct ggataggtga tgagccgaca
     3781 caaccaggaa ttaatgccag attttccaga ccaggcatac cttcctgcaa agtgtatttt
     3841 accaggcgat gccagttttc tccggctcct acatgtaaat accacgcatc aggttcatca
     3901 tgaatttcga tacctttgat ccggttgatg atcaccgtgc cgcgatagtc ttccagaaaa
     3961 agtacattac ttccttcacc cagaataaga acgggttgtc cttctgcggt tgcatgctgc
     4021 caggcattga gtaattgttg ttcgtcttcg gcacatacaa tgtgctgagc attatgatca
     4081 atgccaaatg tgttccaggg ttttaaggag tggttcatag ctgctttcct gatgcaaaaa
     4141 cgaggctagt ttaccgtatc tgtgggggga tggcttgtag atatgacgac aggaagagtt
     4201 tgtagaaacg caaaaaggcc atcctgacgg atagcctt
//
LOCUS       DADLTI010000247         4215 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLTI010000247 DADLTI010000000
VERSION     DADLTI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.251"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..203)
                     /locus_tag="JN755_004027"
     CDS             complement(<1..203)
                     /locus_tag="JN755_004027"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY7382614.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JN755_004028"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JN755_004028"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY7382615.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="JN755_004029"
     CDS             2926..3681
                     /locus_tag="JN755_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY7382616.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="JN755_004030"
     tRNA            3760..3833
                     /locus_tag="JN755_004030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
     gene            3968..>4215
                     /locus_tag="JN755_004031"
     CDS             3968..>4215
                     /locus_tag="JN755_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7382617.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHL"
BASE COUNT         1062 a         1053 c         1080 g         1020 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtc tactaactta ctgaatccta agggatacgg cttaacgcct tccgatagtt
     3901 ttactcactg taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc
     3961 tcagtacatg ctcaagtcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg
     4021 tcttaggatg aagaccacta gtcgcctgac cgcttcgctt ccttctcaca accgttataa
     4081 actagcgtca gtatctttac ggaccaagga tagacgtacc gctatggctc actcacggca
     4141 catcaagtca gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct
     4201 acgggagcac ctgaa
//
LOCUS       DADLTI010000248         4202 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLTI010000248 DADLTI010000000
VERSION     DADLTI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.252"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..488)
                     /locus_tag="JN755_004032"
     CDS             complement(<1..488)
                     /locus_tag="JN755_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7382618.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
     gene            complement(699..2096)
                     /gene="ccp"
                     /locus_tag="JN755_004033"
     CDS             complement(699..2096)
                     /gene="ccp"
                     /locus_tag="JN755_004033"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7382619.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2501..4150
                     /gene="treF"
                     /locus_tag="JN755_004034"
     CDS             2501..4150
                     /gene="treF"
                     /locus_tag="JN755_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7382620.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1077 a         1068 c          982 g         1075 t
ORIGIN      
        1 tgtaccggac cgcacactag gtttggttta tccgttggtt tgcctgcagc ttccatacgc
       61 ttgcgccaac gccatttcat cgccatcccg ccgagcatac aggcctcgga agaaccaatg
      121 gtgttggtgc caacggcctg accatttttc ggcgcaggcg catgccacag atcggcaacc
      181 atatttacgc aacgcaggtc gatggctgcg gattgcggat attcttcttt gtcgatccag
      241 tttttattga tcgacaaatc catcaattta tggacgtttt cgtcgtccca ggtctggcag
      301 aaagtggcta ggttctgacg agcgttgcca tcaagatata attcatcatt gataatctgg
      361 aatgcgacat catcgcgcat ttcgtgcagc ggaaatcgtt ttgactccgc gatagtagaa
      421 atggcctttg cgccaaaacg tgaatcgagt agttctgagc ggaaatccgt taacagcttc
      481 tggtccattt cgaactcctt aaatttattt gaaggcaata aaaaagtagg atttatccgc
      541 aatggaagca aggcattaca ggcttaattt aaataacaaa atcctaagca gaaaaacgac
      601 tttttataat ataaacattt aacatgataa tattaaaaac agactttatt atcgtaataa
      661 aaatcgccca aacattcctg ttcgggcgat caaattaatt attgtttatc ctgcatatac
      721 ggcgtgtaca ccccgttcag actgtgcagg aaagctacga tatcatccac atcctcctgc
      781 ggcagctctt tgcctacctg atagcgcagc atcagtttca ccgccccgtc cagcgtcggc
      841 acgtcaccgc gatggaagta cggtgccgtt aaagcaacgt tacgtaaacc gggtactttc
      901 tgacgcaatt tatcacgctc ttctttagtc acattcatac gaccaatatc cgccgccgta
      961 atttccccaa agttaaagtc ttttttcagc accaacggtt caaaggaacg tccgccgaga
     1021 ataataccac catgacaagt tgcacattta ttatctttaa ataattgata gccttttttc
     1081 tgttgcgccg tcagcgcatt ttcatctcca cgcaaccatt tatcaaatgg ggaatccggc
     1141 gtaattaatg ttttctcaaa ttcagcaatg gcatcagtaa tattttcgcc actgaaacct
     1201 tgcggataaa cttcgaggaa ctgcgcttta agctgcggat ctttttccag cttagcaata
     1261 atttcgtccc aggatttcga cgccatttca atcgggttca acggcggtcc acctgcctga
     1321 tcctgcaatg ttgccgcacg accatcccag aactgctcaa cgttaaatac tgagttaaat
     1381 accgtcggcg cattaatcgg cccaactgcg ccaccaacac caatcgatgt ttttctgcca
     1441 tcgacgcccc ccgcattcaa cgtatggcaa tgagcgcatg aaatggtgct atcagccgat
     1501 aaacggggat cgtgatacag cgcaaagccc aacgccactt tttgcgcatc ggtaggcagt
     1561 ttttgcggga tgggctgcac cggttcattg cgatgctccg gagcagtatc attgctggcg
     1621 taatattccg cgcgctgttt tgcaatccag gccagtattt ccgcccgctc ttcatcactc
     1681 accttacccg cccagtgtag cgcggtataa cgcgttggtg gcatagtttc atactgcatc
     1741 acccattcaa tcttattcag atcgctttgc gaaacgggtt tatcagccag cagtgccgca
     1801 cgcacggcct cgaggttaaa agatttatat ccaagcttaa tgtcgtaatc catcaactgt
     1861 ttcgcgccag gaatataata ataggcgggt aattctgccg aaggcgtgtg gcaatagtcg
     1921 catccttttt cgcggagaaa gcctaaaacc ttattatttt cactgacagc agatgcctga
     1981 acatcggcct gtttactgcg tttattatcg tggtaccaca cataaccaga taaccctaaa
     2041 tagcaaatcg cgacgccagc caggccgatc gcggtaatac gtgagaccat tttcattatt
     2101 cttccctcac ggtggtgagt tattgtgacc cagaaaattt tccagaatgc tgcaaacgtc
     2161 tctgcgagac aaagccatca taaaaaacag ggagatacgc tttttgatag gaatcaatca
     2221 attacatgaa tataaactat caataagata gcctgtgcct attacccaga tcaggaatgg
     2281 catatcatga ttgcggcgag agcaggattg aatgttctga aaatgaaaaa caacaccaaa
     2341 tccgtatttt tataaattcc attgatatta gtgcgttata ttttttgaca agttaataaa
     2401 cgtgatccac cgcacgcttt gtcgcccacc aggcggagcg aatgactacc cttaaagaaa
     2461 agcccgataa ttagcgacga atttcggagg ttggatcctt atgctcaatc agaaaattca
     2521 aaaccctaat ccagacgaac tgatgatcga ggtcgatctc tgctatgaac tggacctgta
     2581 tgaattaaaa ctggatgaga tgatcgaggc agaaccggaa cccgagatga ttgaagggct
     2641 gcccgcctct gatgcgctga cgcctgccga tcgctatctc gaactgttcg agcatgttca
     2701 gtcggcgaaa attttccccg acagtaaaac ctttcccgac tgcgcaccca aaatggaccc
     2761 gctggatatt ttaatccgct accgtaaagt gcgccgtcat cgtgattttg acttgcgcaa
     2821 gtttgttgaa aatcacttct ggctgccgga ggtctactcc agcgagtatg tatcggaccc
     2881 gcaaaattcc ctgaaagagc atatcgacca gctgtggccg gtgctaaccc gcgaaccaca
     2941 ggatcacatt ccgtggtctt ctctgctggc gctaccgcag tcatatattg tcccgggcgg
     3001 ccgttttagc gaaacctact attgggattc ctatttcacc atgctgggac tggcggaaag
     3061 tggtcgggaa gatttgctga aatgcatggc cgataacttc gcctggatga tcgaaaacta
     3121 cggtcacatc cccaacggca accgcaccta ttatttgagc cgctcgcaac caccggtttt
     3181 tgcgctgatg gtggagttgt ttgaagaaga tggtgtacgc ggtgcgcgcc gctatctcga
     3241 ccaccttaaa atggaatatg ccttctggat ggacggtgca gaatcgttga tccctaatca
     3301 ggcctatcgc catgttgtgc ggatgccgga cggatcgctg ctcaaccgtt actgggacga
     3361 tcgcgacacg ccgcgtgacg aatcctggct tgaggacgtt gaaaccgcga aacattctgg
     3421 tcgcccgccc aacgaggtgt accgcgattt acgcgcggga gcggcctccg gttgggatta
     3481 ctcttcccgt tggctgcgtg atactggtcg tctggcgagc attcgtacca cccagttcat
     3541 ccccatcgat ctgaatgcct tcctgtttaa actggagagc gccatcgcca acatctcggc
     3601 gctgaaaggc gagaaagaga cagaagcgct gttccgccag aaggccagtg cccgtcgcga
     3661 tgcggtaaac cgttacctct gggatgatga aaacggcatc taccgcgatt acgactggcg
     3721 acgcgaacaa ctggcgctgt tttccgctgc cgccattgtg ccgctctatg tcggcatggc
     3781 gaaccatgaa caggccgatc gtctggcaaa cgccgtacgc agccggttac tgacacctgg
     3841 cgggattttg gcaagcgagt acgaaaccgg tgaacagtgg gataaaccca acggctgggc
     3901 accgttacaa tggatggcaa ttcagggatt taaaatgtat ggcgatgacc ttctgggtga
     3961 tgaaatcgcg cgcagttggc tgaaaacggt gaatcagttc tatctggaac agcacaaaat
     4021 gatcgagaaa taccatattg ccgatggtgt ttcccgcgaa ggcggcggtg gcgagtatcc
     4081 gttgcaggat gggtttggct ggactaacgg tgtggtacgc cgtttaattg gtttgtacgg
     4141 cgaaccataa tatttttaca gccagccgct aacttcctgc tggctgtaaa attatcctct
     4201 tc
//
LOCUS       DADLTI010000249         4128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLTI010000249 DADLTI010000000
VERSION     DADLTI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.253"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..439
                     /gene="tnpA"
                     /locus_tag="JN755_004035"
                     /pseudo
     CDS             1..439
                     /gene="tnpA"
                     /locus_tag="JN755_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JN755_004036"
                     /gene_synonym="qseD"
     CDS             complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JN755_004036"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7382621.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1846..2565
                     /locus_tag="JN755_004037"
     CDS             1846..2565
                     /locus_tag="JN755_004037"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7382622.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2631..3929
                     /locus_tag="JN755_004038"
     CDS             2631..3929
                     /locus_tag="JN755_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7382623.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1042 a          896 c         1018 g         1172 t
ORIGIN      
        1 gcgcacaccc gatggaactg taaatatcac atagtttttt gcgccaaaat accgaagaca
       61 ggtgttctac agagagaagc gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg
      121 gaaaagcgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      181 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      241 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      301 caggggggac tacgtcgata cagtgggtaa gaacacggcg aagatacagg attacataaa
      361 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctgtc cgggcagccc
      421 gtttacggac cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      481 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      541 tatttattgc acgtcgtcaa ttgcggcgtg ctggctcttc cattgatatc tgctggatta
      601 aatcgtctgg catatgttcc tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc
      661 gggtgttcat tcggtaaata taagcctgaa tggggactgt caatttagca gcatcaaggc
      721 atatcagacg tttggtctgt agttcatcga cgatcgacca gatcggtaac caggcaatgc
      781 cgtatccgtt gagcgccata ttctttaata actcgctcat cgaagagata aatatcgtgc
      841 gcccggtaat acctccaacc tctgccagat gacgattgac caacctgccc atataagatg
      901 tggcggtata gttgagcaaa ggaacctggg gctgagtgat atcgaataca gggtgcccct
      961 gtgcgtcggc ggcacatacc gggtacaact cagattcaaa cagtttcagg cagcaaaaag
     1021 gcgattgcat aagatcttca tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta
     1081 acgtatttac ggcctggaca acatcaatgg cttcaacatg atagacaaat tctccgccat
     1141 aggcggtaag tgaatgcacc agtttgggta atacgctcag ggatagtgag tgcgcggctg
     1201 caattttaat gttgggaacg ccgagtaaac tctggccgtt taactccccc aaattgcatt
     1261 caagctgctg taataagctg cgagtctggg aatgaaagag cttgccctct tcggttaatt
     1321 gtaaaggggt ggtcgtgcga tcgaataaaa caacgccgat cgctgcctcc agcgccttaa
     1381 ttcgtcggct gaatgcgggt tgcgaaagat tacgctcttt tgccgcctga gaaaaatggc
     1441 gacaggcttc cagtgtcagg aaatcgtata accattttgt ctcgatattc ttcattacca
     1501 tggcacctgt attttcaatc caaagacata ataaaacatc ccggcaataa aaataatgtt
     1561 ctcaattagc acaacctggc ctttatcatt cgttttgtgc ataacccctt catttccggt
     1621 attgacgttg atatcacatt tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat
     1681 acatcatcac tatggtgaat aagcggaatg ttccatagct gcatcatgcg tgtcttgcat
     1741 cgccttgtac aaccttgcat ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca
     1801 gttatttacc gcgtattgtc attaacgttt tgagaggcag gtattgtgaa aggtttaatt
     1861 ggtgtactcg gtggaatggg gccggcagct accgtcgacc tgtttaataa atttgtaact
     1921 tttaccgctg cacaatgtga tcaggaacat attcctttaa ttatttcttc gatcccggat
     1981 attcctgacc gtaccgatgc attaatgcat catgggcgtt caccattacc cgttatgcgt
     2041 gattatatgc ataagctgga agatgccggg gcagaatgta ttgttattcc ctgtaatacc
     2101 gcgcattact ggttcaaaga attaaaagat gcttgtcata ttgatatatt aagtatcgta
     2161 gaaactacga tcaatgaagt tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc
     2221 aatgccacgc tatatatggg tttataccaa aaaggaattg agtcgctggg atttacctgt
     2281 gtcagccccg atgccgacgg tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct
     2341 ggcaatatag cgcacgcaca aaagttgatg aatgagcagg ctgaaatatt attttcccgc
     2401 ggtgcagaaa tccttgttct tggttgtaca gaaattccgg ttattctggc gcaagccgtt
     2461 aaagagcagc cttcccgcta tattgactca acggcgtcac tcgttcgtgc cggaataaaa
     2521 tggtacgaaa atcgtgtcgg taaacattat cttttaaccc aataatcatt taaatcgcag
     2581 ccagaaagtt attcgctttt aactgaatta tatttataac ggagaacatt atggtttggc
     2641 tggaaattat cgtagtactt ggtgcaatat tttttggtat tcgccagggg ggaatcggta
     2701 ttggtttatg tggcgggctt gggcttgcca ttctgactct gggatttggt ctgcctatgg
     2761 ggtcgccgcc agtcgacgtg atcctgatta ttatgaccgt tgttgtggcg gcctcagcct
     2821 tacaggccgc aggcggaatg gattatctgg tacgcgtcgc tggtaatttt atgcggcgta
     2881 acccgaaata tatcaacata atcgcgccga ttatcacctg gtctatgacc attatggccg
     2941 gtactgggtt tattgttttt tccacgctgc cggtgattgc tgaagtggcg aaagagtccg
     3001 gtatccgtcc ttcccgcacg cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg
     3061 gttcgccgat cagcgccgca atggcggcta tgctcactat tatggaggta aacggtgtca
     3121 gttttattca agtgatgtcg gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg
     3181 cttttattgc ttcccgacag ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc
     3241 tgcaaaaagg actggtgcaa aaatacgaga ataatagcag catcaagcca ggtgcgacgc
     3301 tctccgtcgg attgttcctg ctggcgacgg tcgctattgt tattcttgct gccttccccc
     3361 aactgcgtcc gggttttgat atcggtaaac cgatggaaac acgcgatatc attattatct
     3421 gtatgctctc tgccgcctgc ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta
     3481 ttctcacctc cacattccgt gcggggatga gttcgctggc ggtaattctc ggcattgtaa
     3541 ccttaggcac cacttttatt gatgcgcatt taactgagat taaagatatt gctggtgata
     3601 ttttgcaggc gtatccgatg ttgttggcgc tggttctttt tggcacttgc gcgctgctct
     3661 actcgcaagg tgcgaccacg ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca
     3721 cctgggcaat tctggcgtcc tatgtggccg tcaccggagt atttgtactg ccaacgtatc
     3781 caacatcact ggctgccatg gaatttgata ctaccggcac aactcgtgta ggcaaatatg
     3841 tgctgaatca tccatttatg ctgcctgggt tggggggaat tattgctgga gtggcattag
     3901 gctttgtgat tgcgccaatg attatttagc actatcaaac atcagtatcg atggtttata
     3961 cacaataaag acagatatgg cggaatatag tctgttgtaa gcgccaacaa agaatagggg
     4021 aattccgcca tgtctgaaca tcttcacaat atggtattaa gtgttgtcgt ttaatgattt
     4081 atttcttggt tatgttgtgt attagctgca tctatcgctt tttgatac
//
LOCUS       DADLTI010000250         4113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLTI010000250 DADLTI010000000
VERSION     DADLTI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.254"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            244..1199
                     /locus_tag="JN755_004039"
                     /pseudo
     CDS             244..1199
                     /locus_tag="JN755_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..2101
                     /locus_tag="JN755_004040"
     CDS             1196..2101
                     /locus_tag="JN755_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY7382624.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2098..3168
                     /locus_tag="JN755_004041"
     CDS             2098..3168
                     /locus_tag="JN755_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY7382625.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3304..3981
                     /locus_tag="JN755_004042"
     CDS             3304..3981
                     /locus_tag="JN755_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382626.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1071 a          968 c         1139 g          935 t
ORIGIN      
        1 tcacttatcg atcaactgat aacggaagtc atactgagtt cacactccag agctgcggca
       61 ctggcggttg attctgccgc cgcaaaactg atgcagaatg cagacaatat cagtgaatac
      121 ctgtcgttac accatcaggg gctgatgcaa agtattcagt cactgcaggc gcatatcacc
      181 agcctgcttg aggatatccg ggaaatagca gactgtcagg agcaggtgac cgctgatgtc
      241 agaatggcca tggaggagat cgatgccaga acccgggaat tactgacggg gtctgcacct
      301 cactggaaga ggagctgaat gactatttca gaagcggtaa acgcaaagaa cagcaaatgc
      361 tggaggaaga aaattcagcg cagccccggg agagaaacgc attcgctttc tttcacgata
      421 ttttcggaac cggaaatcag catgacagga tgcgggactt tgatccggac agccccgaaa
      481 taaaattcag tgatcgcagg gaagcccttg aactgatgac gcaaatcgaa tcgaccgtga
      541 ccagcctgca ccgagaggct gaagcacagt tccggcctga gctggagaaa atcgtcagcg
      601 ggattgaaac aggttttcgt ggcacggccc tgtacgccac agaaaacatt gccggtcgca
      661 tcaatacccg cctggaggat gagggcttta ccgtaaaaat cagttttccg gcagtcagtc
      721 agttacaaac ccggctcgcg gtaaaaacaa atctgagtgc gcttatggag gaaagaacgg
      781 agaccgtcac ccgtcgccgt cggcagcgtg gtttatgggg aaagatttgt ggagcgtttg
      841 gcaccagtga ctggggctgg gaaacctata aagaggatgt gagccgcagt gttatcaata
      901 tcaacaccgt caggaaggaa gttatgtcac tgacccgggc atatttcggg gagctgcagg
      961 catccattga gcaggatatt aaccagcccg tccgccagga aatcgatgcc tttttctgcg
     1021 cattcaggga gaaagttgaa caactgcgta acacgctgat tcagagctct gaagatcata
     1081 aacgcgatca gcaggcacag gaacggctta ccaggcgact tcaggcatta aatgaaaggg
     1141 ttcctgagct cattactgac agtaaggcgc tgagggaaga gctggagaca atgctgtgac
     1201 ttcaccattt attcaacaaa ttgctgataa ccgggtatgt caggtactca cctgtcttcc
     1261 tgaaaaattt gtggttgatt tcgcaaacgg aatcgatgtt gcacaggagc atatccgcac
     1321 ggccggagag cgcacgttct tccggcgttt aaaagaggga ctgaccggcg aaggcgctgc
     1381 acggcagaat gccatcaatg cttcggttgc acagggcgtt gaggcgtccc tgcgctggct
     1441 gacggagctg acaacgtcac ttgccaccac aaactacgcg attacccggg taaacgacag
     1501 ggtcagttca ctggtcagtg atactgccag gttggcgcat tattccgcag atacacggga
     1561 gcagttactc atcctggccg atcaggttca ccataaactg aatcatcttg aagaaaaact
     1621 ccaccgtgtt gaccaggttc agcgggcgca gttacatctt gagcagatat tctcatggtg
     1681 gagcgccggg cgatacgcgt cattttcccc tgccggacgt tgttatgtgg cacttgaaga
     1741 gcttcgctgg ggcgcgtttg gtgatgtgat acgtcagggc gaaacaggcc aggttaacca
     1801 gctactggat attctcagac ataaagcatt aacgcagatg gcacaggaga gtggcggtag
     1861 tgcaaccgtg cgtctgaaca ctctggactg gcttggtggt cagggccggg agcaggcaga
     1921 taacgagtgg catgacgcga ttaactggct gggagactgg tgcagtgaag agcagcatcc
     1981 ggtgatctgg tcaaccacgc aggctgcaga acacttaccg gttcgcatgc ctcgcctctg
     2041 ctctgcagaa cgcctctctg aaagtatggt cgatgaaata tttcagaagg gggctgcatg
     2101 agtacagaaa tgaaaacggg gctggtgctg tccggtggcg gggcggtggg cgcttatcag
     2161 gcgggagtgg ttaaggcact ggcagagtgt gatacacaga tcagcatggt ttcaggggcc
     2221 agcattggcg cattcaatgg tgccattatc gcgacctctc ccgatctgtc agaagctgcc
     2281 gtacgcctgg aagcgctctg ggatcatctg gggaataatc aggtgctgtc ggtaaaccga
     2341 ttggtttact tttcattgct gaaaaaattg ttccaggcaa tgaacctctg ccagatcccc
     2401 ggacgtgcag gagcactgct tacgacgctt cttcgccata tatcgatact caacgggttt
     2461 gacaatctga tggctcagcc gttgttgtca gatgagcccc tgacagcgct gttggatcat
     2521 tatcttgata ctgatgctct ggcagacggg ctaccgctgt atgtgtcgct gtaccccaca
     2581 gaagggggca tgcaggatat tattgactgc attcgtgctg aactgggtgc cggaaccacg
     2641 aaaaacgccg tttttcagca tatccagagc ctgccccgcg gacagcagaa agaggctctg
     2701 cttgcgtcag ccgcgctgcc cctgctgttc cgtccccgtg aggttcaggg gacaatgttc
     2761 ggtgatggtg gtatgggagg atggcgaaat atgcagggaa atacccctgt gacgcctctg
     2821 gtcgatgccg gatgcaatat ggtgattgtg acgcatctga gtgacggttc tttatgggat
     2881 cgccaggctt ttccggacac cacaatcctt gagatccgtc cccggaaaag gctgaaatat
     2941 gcaggtgatg gtgacaacag cggcggtctg ctcagtttca cgttggcaca taccgacgcc
     3001 tggcgtcagc agggctatga agacacgatg ctgacgatgg agcatatccg gaaaccgctg
     3061 gcagcacgtc aggtactgtc ccggtcagaa acggtgttgc agaaaagtct ggagataacg
     3121 gaagaggcag atttggcact gagaaacgcg atggcccgga ttaaataaag acgctccaaa
     3181 gaaacgccac cgcctgtggt ggtgtttttc ctagctggtg ttattgaaaa tattttcaga
     3241 tatatattac ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta
     3301 attatgcaat tacctgtaaa gttattaatg agccttatat ctctggtcag cgttattgca
     3361 cgtgccggga aatataaaaa ttatatccgg gatgaaataa aatactggcg atatacatca
     3421 tacaaggggg ggaaatttcc ggaaggtttc actgatgaga aattttccag cgccatttac
     3481 aacggaagaa tatttacaat gaaacgttta cataccctga tgttatttct ggcggttctg
     3541 tttactggct ttaacgtgga agcagcgagc gtgaaacaag cgctcagctg cgacccaaac
     3601 gcccgggctg aacaacctgg agcgtgtcca acaacgtacg agttgtacga aggtgacgct
     3661 gcctacaaag ctgcgcttga caaagcatta aaaccggtcg gactgagcgg catgttcggt
     3721 aaaggcgggt atatggatgg ccctggcgga aacgtaacgc cagtaaccat taacggtaca
     3781 gtctggctcc agggcgacgg ttgcaaagcc aatacctgcg gctgggactt tatcgtaaca
     3841 ctctataacc caaaaaccca tgaagtcgtt ggctaccgct actttggttt agatgacccg
     3901 gcctacctgg tttggttcgg cgaaattggc gtgcatgaat tcgcgtatct ggtgaaaaac
     3961 tacgtagctg cggttaacta atttaaggag tctgtcatga aaacttttat caaaacttta
     4021 ctcgttgctg taactattct gttctctgtc ttcgctacgg cgaaacaagt aaaactgcca
     4081 aacaacatca aatacgttaa tactacagag gcg
//
LOCUS       DADLTI010000251         4109 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLTI010000251 DADLTI010000000
VERSION     DADLTI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.255"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            80..1606
                     /gene="mdtQ"
                     /locus_tag="JN755_004043"
     CDS             80..1606
                     /gene="mdtQ"
                     /locus_tag="JN755_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7382627.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            1659..2420
                     /locus_tag="JN755_004044"
                     /pseudo
     CDS             1659..2420
                     /locus_tag="JN755_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(2537..3115)
                     /locus_tag="JN755_004045"
     CDS             complement(2537..3115)
                     /locus_tag="JN755_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7382628.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3285..3872
                     /locus_tag="JN755_004046"
     CDS             3285..3872
                     /locus_tag="JN755_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7382629.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            4046..>4109
                     /locus_tag="JN755_004047"
     CDS             4046..>4109
                     /locus_tag="JN755_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7382630.1"
                     /translation="MPKFRVSLFSLALMLAVPFAP"
BASE COUNT          970 a         1047 c         1120 g          972 t
ORIGIN      
        1 tgccgttatc cataaacgat taagataaat cggaagatat catttactta gtctatattt
       61 ttctgggtat gtcgttttat tgctactgat gtattgccac gcgaaactaa aaaatatatc
      121 gcaacacacg gtaatctccg cgcaccgttt cttacctgat tattccccca tgaatcgtga
      181 ttccttttat ccagccatcg cctgttttcc gctgttactg atgctggccg ggtgtgcgcc
      241 tatgcatgaa acccgccagg cgttaagcca gcaaacgccc gctgcacaag ttgacaccgc
      301 attacccacg gcgctgaaaa atggttggcc agacagccaa tggtggctgg agtatcacga
      361 taatcagctc acttcattaa ttaacaatgc actacagaac gcaccggata tgcaggtggc
      421 tgaacagcgc attcagctag ctgaagccca ggcgaaggca gttgccactc aggatggtcc
      481 gcagatcgac ttttcggcgg atatggagcg gcaaaaaatg tcggcagaag ggctaatggg
      541 gccgtttgct ctgaacgatc cggccgcagg tacgaccggt ccgtggtaca ccaacggaac
      601 ttttggctta acggcgggct ggcatctcga tatctgggga aagaatcggg cggaggttac
      661 tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc gagcaaaccc gccaattgct
      721 ggctggcagc gtagcccgcc tgtactggga gtggcaaacc caggcggcgt taaacacggt
      781 cttgcagcaa atagaaaaag agcagaacac cattatcgcg accgatcgcc agctatatca
      841 gaacgggatt acttcttcag ttgacggtat ggaaaccgat attaatgcca gcaaaacccg
      901 gcagcagctc aacgatgtcg tggggaaaat gaaaattatt gaggcacggt taaatgcact
      961 cacaaatcac cagactaagt cattgaagct taaaccggtc gcgttgccga aagtggcaag
     1021 ccagcttcct gatgaactgg ggtactcctt actggcccgg cgggcagatt tgcaggcggc
     1081 gcactggtac gttgagtcat cgttaagcac tattgatgcg gcaaaagcgg cattttatcc
     1141 tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg cacttaagcg atctgttccg
     1201 tcattccgcg cagcaaatgg gcgttacggc aggcctgacg ctacctattt tcgatagtgg
     1261 tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc aacttgtcta tcgccagcta
     1321 caacaaagcg gtggttgaag cagtgaatga cgtggcgcgg gcagccagtc aggttcagac
     1381 actggcggag aaaaaccagc atcaggcgca aattgagcgc gatgccttgc gtgtggtagg
     1441 tcttgcgcag gcccgcttta acgcgggcat cattgctggt tcccgcgtca gcgaagccag
     1501 aatcccggcg ctgcgtgagc gggccaatgg cctgttattg caagggcagt ggctggatgc
     1561 ctccattcga ctcaccggtg cgctgggcgg gggttacaaa cgctgatgat atgcgccttc
     1621 tatacttaac gtttattcag cgttaagtgg agaactcgat ggcataggtc gcgataatta
     1681 ccgcttccga ttcggggatc ggcaaagagt gcgcgttatt actggcgcat caggggtttg
     1741 atattggcat tacctggcac tcagatgaag aaggggcaaa agataccgcg cgtgaggtgg
     1801 ttacccacgg cgtacgtgcg gagatcgtgc aactggatct cggcaatctg ccagaaggtg
     1861 cgcaggcgct ggagacactc atccagcgat ttgggcgtat tgatgtgctg gtgaataatg
     1921 cgggtgcaat gaccaaagcg ccgtttcttg atatggcttt tgatgagtgg cgcaagattt
     1981 ttaccgttga tgttgatggt gcatttttat gctcgcaaat tgcggctcgt cagatggtga
     2041 aataagggca gggcggtcgc atcatcaaca ttacgtcggt acatgaacat acgccgctgc
     2101 cggatgccag cgcctacaca gccgctaaac atgcgctcgg cgggttaacc aaaacgatgg
     2161 cgctggagtt ggtcaggcat aagattttgg tgaacgcagt cgcgcctggg gcgatagcca
     2221 cgccaatgaa cagcatggat gatagcgacg tgaagcccga cgcggagcct tcgattcctc
     2281 tacggcgttt tggcgcaacg catgagattg ccagcctggt ggtgtggctt tgttcggagg
     2341 gcgcaaatta cacgacaggg cagtcgttga tagtggatgg cggctttatg ttggcgaatc
     2401 cgcagttcaa cccagaatag cgcagtgagg tgcgtggata tttcaatggg ttaggccaga
     2461 taagacacca gcgtcgcatc aggcatggaa cacccggtgc cggatgtgga gtgaatgctt
     2521 atctgtggca actgctttac gcctgattat ccggcttttt cttcccgcgc cgtttcagcc
     2581 accagcgcac gcccaccacc agaaccacga ccagaatcaa ccagacccag tgcttcaaat
     2641 gctggtcgag attgtgcaac cacggcgcaa tcacctgacc accagcgtaa ccaatagtgg
     2701 taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag attttcggcg
     2761 gcagctggct ggcaccaatc agcgtcgggc caatcacccg aaagccatac ataaagcgcg
     2821 taccaatgac aaacagatac ggatggcgtt ggataagttt ctgcgcccgc tcaattttat
     2881 cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa ccgccgcccg cacagataga
     2941 gtacctggtc gccaatcatg ccaccaagcg ccacagaaag taccaccagt gggaacttta
     3001 atagcccctg atgtgccgca acgcctccca gcaaagtcac ggtttcacct tccgctaggc
     3061 taccgataac cagcgcggca taaccatatt gtgagatgag tgtattgaga tccataggcc
     3121 agtctgtctc cctttggttg cctgtaagca tacaccctgt gaatcattca cgctgaaaaa
     3181 ggacatacat cagacggtgc aaattacatg gatgaacaaa aaataatcac aagctggctt
     3241 atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc catgtctggg
     3301 gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac gaaacgattt
     3361 ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt tgcgccttca
     3421 ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag ttatcctggt
     3481 ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg gttgcggtga
     3541 tggggcgggt catctggtgg atggcgcgca attacccgca gcgtccgtct cttgcgcact
     3601 gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg gtggcgcttt
     3661 atccactggt ctggttgtgt gcgctggtcg gcacggtggc gctgttttac accggctacc
     3721 tgctttacct tggtattccg agcttcctga atatcaacaa agaggaaggc cttagcttct
     3781 ccagttcgac gctggcgatt ggcgtgctgg tactggaagt gctgctcacc ctaaccgtta
     3841 tactatgggg ctacggttat cgtttgttct agaaagttct ttgacgttgc attgctggcg
     3901 taaatgtcag caatgcagca tttactcacg attctccttt gacgatcgca ctttttgctc
     3961 gttatgatgg cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca acccgtgcgc
     4021 gtgaaccact atctgaatgc tcatcatgcc gaaatttcga gtttctttat ttagcctggc
     4081 cctgatgctg gctgtgcctt ttgcaccgc
//
LOCUS       DADLTI010000252         4075 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLTI010000252 DADLTI010000000
VERSION     DADLTI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.256"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..113
                     /locus_tag="JN755_004048"
     CDS             <1..113
                     /locus_tag="JN755_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY7382631.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JN755_004049"
     CDS             complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JN755_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7382632.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JN755_004050"
     CDS             complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JN755_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7382633.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JN755_004051"
     CDS             complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JN755_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7382634.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JN755_004052"
     CDS             complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JN755_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7382635.1"
                     /translation="MLLGCLALTCSITFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1109 a          997 c          996 g          973 t
ORIGIN      
        1 tgcaggcgca ggtcgatcgc aacgatgaga tttatcgccg tcagcgggag ctgaaggaag
       61 agctgaatag tctgaaggat ttgaattcgg ttaggaattt tatcgtggaa taacagaagc
      121 tgcggcacgt cgtatgcagg aacgtgccgc ggttggctgg taaactttcg atagtgcgag
      181 tattgaatga tttctaggcg ttatcgattt tacgtatttt ttgcatgata gaattcgtac
      241 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
      301 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      361 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      421 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      481 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      541 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      601 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      661 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      721 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      781 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      841 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      901 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      961 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
     1021 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
     1081 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
     1141 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgcacg
     1201 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1261 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1321 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1381 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1441 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1501 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1561 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1621 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1681 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1741 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1801 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1861 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1921 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1981 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     2041 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     2101 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     2161 gtaaaagtgg tgtctgtcgg cccgcttgcc aggacggtgc ctttgaccat gaccgtataa
     2221 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2281 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2341 aaaggttcat ccagcaggat cacatcaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2401 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2461 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2521 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2581 tcaacctctt ctgactgagg gacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2641 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2701 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2761 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2821 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2881 gtaccagcgt actggtagta accttaagaa ggtcgatata cgtaggtacc gggccgtttt
     2941 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     3001 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     3061 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     3121 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     3181 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3241 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3301 ttgccggatc gtattttatc aacgcatcac gaatattatc gacgtaaatc agagcattat
     3361 ctggcgacat ccatgcatgg ggattaggtt tgccctcata gggtccttcg gtgatcccta
     3421 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3481 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3541 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3601 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3661 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgtgatcgag caggtgaggg
     3721 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3781 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3841 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3901 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3961 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa aggggcaaaa
     4021 aagagtgggt tatcgtagct tattgttgtc gctgataatg tttaacatat tgaaa
//
LOCUS       DADLTI010000253         3959 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLTI010000253 DADLTI010000000
VERSION     DADLTI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.257"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..123)
                     /locus_tag="JN755_004053"
     CDS             complement(<1..123)
                     /locus_tag="JN755_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7382636.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAP"
     gene            336..1799
                     /gene="bepA"
                     /locus_tag="JN755_004054"
     CDS             336..1799
                     /gene="bepA"
                     /locus_tag="JN755_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7382637.1"
                     /translation="MFRQLKKNLVAALIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            1820..2179
                     /gene="arsC"
                     /locus_tag="JN755_004055"
     CDS             1820..2179
                     /gene="arsC"
                     /locus_tag="JN755_004055"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7382638.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2312..3058)
                     /gene="hda"
                     /locus_tag="JN755_004056"
     CDS             complement(2312..3058)
                     /gene="hda"
                     /locus_tag="JN755_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7382639.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3108..>3959)
                     /gene="uraA"
                     /locus_tag="JN755_004057"
     CDS             complement(3108..>3959)
                     /gene="uraA"
                     /locus_tag="JN755_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7382640.1"
                     /translation="LPAEGQTPNSKTIIISITTLAVTVLGSVLFRGFLAIIPILIGVL
                     VGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIAEHVGHLVV
                     TANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVI
                     GGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQ
                     NLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIGLSLIFKLISVLRPEEVVLDAE
                     DADITDK"
BASE COUNT         1057 a         1049 c         1022 g          831 t
ORIGIN      
        1 cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag
       61 caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag
      121 cataaagacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt
      181 cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg
      241 ctaatcttcg ccgttacact caaaggcggc gaggtgggaa cgatatttca cagtatcggt
      301 caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc
      361 tggttgcagc cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca
      421 gcgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg
      481 aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg
      541 acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt
      601 cggttaagac accgtttcat ttttttctga tcaacaacga cgaaattaac gcctttgctt
      661 tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc
      721 aactggcttc agttatggcg cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag
      781 cgatggaaga tcaacaacgc agcgcgccgc tgacctgggt cggcgcgtta ggttctattt
      841 tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa
      901 cgcgccaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta
      961 ttcaggtgct gcaacgctcg ggattcgatc cacaggcgat gccgaccttc ctggaaaaat
     1021 tgctcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc
     1081 cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc
     1141 agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac
     1201 gtaaccagct caccagtaat ttgctggatg aatgggcgaa aggaaacgtt cttcagcaac
     1261 gagcggcgca atatggtcgt gctttacagg cgatgaaagc caataaatac gacgaggcgc
     1321 gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg
     1381 ctaccgatat cgatcttgag caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg
     1441 cccgcgattt acgcaccaat ccggtgttgc agctcaacct ggcgaacgct tatctgcaag
     1501 gcggtcaacc acaagaagcg gccaatattc tgaatcgcta cacctttaat aataaagatg
     1561 acagcaacgg ctgggatttg ctggcacagg cggaagccgc gctaaataac cgcgatcagg
     1621 agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc
     1681 tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg cgttacgatg
     1741 cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat
     1801 tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc
     1861 aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag aagtggtgct
     1921 ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat
     1981 gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac taaacctggc
     2041 tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat
     2101 ggagcgcccg attatggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt
     2161 actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg ccggatgcga
     2221 cgcttgccac gtcttatccg gcctacgaat actatggatt aacttcgcgt aggtcaggcg
     2281 ttcgcgccgc atccgacaat aaacacctta tctacaactt cagaatttct ttcacaaacg
     2341 gaatggtcag cttacgttgc gcggtaatcg acgcacgatc caactgatcc aacgtcataa
     2401 atagcgtgcg catttctctg tcgagccgct tcagcaagaa acgccccaca tcttccggca
     2461 gttcaaaacc acgcaaacgc gcgcgtaact gtagcgcctg caacttatct tcatcagaaa
     2521 gtggctgcaa tttgtagatc tgcccccagt cgagacgcga cgcgagatcc ggtaatccca
     2581 gattcaactg ccgcggtgga cgatcgccgg tgatcaacaa ccgtgttttg cccgattcca
     2641 gaattcgatt gtagagatcg aaaatcgcca tctcccacaa ctcatcgcct gcaatacact
     2701 caatgttgtc gatacagacc agcgacaaat gctccatacc gtcgagcact tccggaacaa
     2761 accaggtgcg tttatccagc gggacatagc ccaccgcatc gccacgctgc gacaattccg
     2821 cgcaagccgc gtgcagcaga tggctgcgcc ccgcgccttc gcgtgcccag agatagatgt
     2881 aaccgctatg ttcctgacgc agcacgtttt gcagcgcggc cagtaaagag gagttatccc
     2941 ccggccagaa acttgcaaag gtttcgtcgt caggaagata aagtggcaaa gagagctgtg
     3001 ccggtgtgtt cagagatacc tcaaccagga tttcacaaaa tcgcgagaag tttaccacag
     3061 aatcccatga tgtttgaacc gggcagcaac actgcccggt cggtacatta tttgtctgtt
     3121 atatccgcgt cttctgcgtc cagcaccact tcttcagggc gcagcacact gatcagtttg
     3181 aaaatcaaac ttaagccgat accgacgatg gtcgccagcg ccataccttt taactccgcc
     3241 gcgccgatgt ttaccttcgc gccactgacg ccgatgatca aaatcacgga agtcaggatc
     3301 aggttctgtg ctttgttgta atccactttc gattcgatca aaacacgaat accggaagca
     3361 ccaatgacac cataaagcag cagcgaaacg ccgcccatca ccggcaatgg gatcatctgg
     3421 atagcggcag ccagtttacc gacgcaggaa agcaggatag cgaaaatcgc cgccccgccg
     3481 ataacccagg tactgtaaac acgggtgatc gccatcacgc caatgttttc tccgtaagtg
     3541 gtatttggcg tagagccaaa gaagccggag atcacggtcg acaggccgtt agcaaacatc
     3601 gaacggtgca gacctggatc gcgcagcaga tcttttttga cgatattagc cgttactacc
     3661 aggtgcccta cgtgttcggc aataaccact aacgccgctg gcagaatagt cagaatggca
     3721 aaccactcga agcgcggcgt atagagggtt ggcagtgcaa accagtgagc attaataatc
     3781 ggcgtggtat cgacaattcc cattgcgaaa gagagcgcgt accccaccag cacgccaatt
     3841 aaaatcggga taattgccag gaaaccacga aacagcacgg aacctaaaac cgtgaccgcc
     3901 agggtggtaa tagagatgat gatggttttg gagtttggcg tttgcccttc agccgggag
//
LOCUS       DADLTI010000254         3951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLTI010000254 DADLTI010000000
VERSION     DADLTI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.258"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..2301)
                     /locus_tag="JN755_004058"
     CDS             complement(<1..2301)
                     /locus_tag="JN755_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY7382641.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRNPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHL"
     gene            complement(2304..2864)
                     /locus_tag="JN755_004059"
     CDS             complement(2304..2864)
                     /locus_tag="JN755_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7382642.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2990..3340)
                     /locus_tag="JN755_004060"
     CDS             complement(2990..3340)
                     /locus_tag="JN755_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7382643.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3543..>3951)
                     /gene="intI1"
                     /locus_tag="JN755_004061"
     CDS             complement(3543..>3951)
                     /gene="intI1"
                     /locus_tag="JN755_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7382644.1"
                     /translation="QAEGRSGVALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGV
                     VRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHS
                     DVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT          710 a         1156 c         1270 g          815 t
ORIGIN      
        1 gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc gtgtcggtgt agtgctcctc
       61 gatccgcagg tcggattcgt ggtacagcag gccgtcgagc acgtaggttg agtcgcgcag
      121 gccgacattg accaccttgg tgtggaatgg cgcgtattgg tcggagatgt gggtgtagaa
      181 agtccgtcct gggctgctgc catattttgg gttgatgtgc cccgtgctct ttgccttgct
      241 agcggttcgg aaattctgtc cgtccgatga tgatgtggtg ccatcgcccc agtgcccggc
      301 aaagggattc cgaaactgag cgttgaccag ttcagccaac gctgtcgagt acgtttcgtc
      361 gcgggtatgc caggcttgca gccaagcgag cttcgcgtag gtcgtgccgg ggcaggactc
      421 ggccatcttg gtcaggccca ggttgatcgc gtcggccagg atcgtggtca acaacaggtt
      481 cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag tgccgggtga agcccgtcca
      541 ctcatcgact tcgagcagca gttcggtgat cttgacgtgc ggcaggacca tggctgtctg
      601 gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc agcggcgtga tcttcaagcc
      661 cgactcggtg atgatggcat ccggcaggtc gttggctgcc gccatgcggt tgacggtggc
      721 aagttgtgct tccagcagcg tcagccgctc atgcagatat tgttcgcagt cggtggccac
      781 ggccagcggc aattcgctgg actgcttgag gctggtgaac ttctcgggcg gtaccaggta
      841 gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag atgtcgcccg agcgcaggga
      901 gttcttcaac tcggacagcg cgcacagttc gtagtagcgc cggtcgatgc cggcgtcggt
      961 catcaccagt ttctgccagc gcggcttgat gaagccggtc ggtgcatcgg ctggcagctt
     1021 gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg gcatcaagca cgtttttggc
     1081 ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt
     1141 ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg
     1201 cgcctcggtg acgctctcgg caaaggaatc ccaggacatg acggcctcga tggcggcaaa
     1261 cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg
     1321 taccttggcg ttgatggcct tgcctgacgc ctggaactgc tgctgatgct tattcttggc
     1381 agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt
     1441 ggccatgccc tcggtggcca gcgccacgag agtggcgtag cgccgttgcg gctcgaattt
     1501 ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca atcttgagca ggcggttctg
     1561 gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc
     1621 gatgtgttcc agcatatgcc gcgaatttgg cttggccgga gactggcgca accaagccaa
     1681 ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc
     1741 gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc
     1801 ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg accgactgcc gccgcaggtg
     1861 cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag
     1921 catctggacg gcctgccggt aatggctcat ggtgaagggc cggaaaccga acacggtttg
     1981 cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg ccgtactcgt tccagctttc
     2041 gacgccgacc ttgagctggt cggcgaccag cttcaacaag ggcgggaacg gtagttcatc
     2101 gacgcccagg atgacgccgg gaaagcgcag gtaacagagc tgcaccgcga agcccagccg
     2161 attggctggc ccgcgccgct gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg
     2221 tcggatcagg tcgtccttgg agtccggcaa cgccagcagg ctttcccgct cggcggcgga
     2281 caggatggaa cgacgtggca tatttactga tccgttctca agtattgata cagggtttcg
     2341 cgactgattc cgaattcacg agcaagcttg gtcttttgct cgccagcctc gacacgttgg
     2401 cgcagttcgg caatacgctc agacgacagg gatttcttcc tgccacggta agccccgcgt
     2461 tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg
     2521 gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa
     2581 ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc
     2641 cggcgcaaat catcgagatt gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg
     2701 ccggtgcggg cgaagcttat cagcgcttcc agttgcggac gcttgacatc cttgccggat
     2761 gccttgtcgc taaaagcgcg atcaaccttg acgccttcca gttgccgttc cgggttctgg
     2821 tcgaaggtgc tgaccctgat atacccaatg cgctgtccag tcatggaatt ccctgcaaaa
     2881 tgtcagggaa gactctatga ccttcaacga gatatgtcaa taaattcaaa attcaatcct
     2941 atcctgacgc aatttacaca tggcatctga catcaggtta gggtatgcct caacctgacg
     3001 gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct
     3061 ggaagcgctg atagcactcc agcccgcaga aatgctccac gtactcggcc ccttccggcg
     3121 tgaaggcggc atcgagcggg atttccttgc agcacacgca gcaactggtg ctcggttcat
     3181 tggcgttcat ggtggtgttc ctccattggt tgacgaagcc gacgaaggcc gccgccggca
     3241 tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa
     3301 ggcagtcggg tgtttccaca ccctcggcta ccacctccat tgtcgttttc agaagacggc
     3361 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat ccatcaggca
     3421 acgacgggct gctgccggcc atcagcggac gcagggagga ctttccgcaa ccggccgttc
     3481 gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc gccaggattg acttgcgctg
     3541 ccctacctct cactagtgag gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg
     3601 ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga
     3661 tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg
     3721 agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg
     3781 cgctgaaagg tctggtcata catgtgatgg cgacgcacga caccgctccg tggatcggtc
     3841 gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg aatgcccggc gcgcggatac
     3901 ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc cctcggcctg g
//
LOCUS       DADLTI010000255         3913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLTI010000255 DADLTI010000000
VERSION     DADLTI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.259"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1223
                     /locus_tag="JN755_004062"
     CDS             <1..1223
                     /locus_tag="JN755_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7382645.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1220..2185
                     /gene="yggF"
                     /locus_tag="JN755_004063"
     CDS             1220..2185
                     /gene="yggF"
                     /locus_tag="JN755_004063"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7382646.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2207..2716
                     /gene="fumE"
                     /locus_tag="JN755_004064"
     CDS             2207..2716
                     /gene="fumE"
                     /locus_tag="JN755_004064"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7382647.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2713..3426
                     /locus_tag="JN755_004065"
     CDS             2713..3426
                     /locus_tag="JN755_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7382648.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3398..>3913)
                     /locus_tag="JN755_004066"
     CDS             complement(3398..>3913)
                     /locus_tag="JN755_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7382649.1"
                     /translation="PTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADE
                     IAFGLKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPP
                     LLLLDEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVI
                     RNVNPPDDIHP"
BASE COUNT          912 a          921 c         1150 g          930 t
ORIGIN      
        1 ttgaactgcc agaaattacc gataatgaac tgttagtgag tgtaatttct gacagcgtct
       61 gtttatcgac ctggaaagcg gcgttactcg gtagtgaaca taaacgcgta cccgacgatt
      121 tagaaaatca tccggtcatt accgggcatg aatgtgccgg gattattgtc gaagtgggta
      181 aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt
      241 taccaagcgg atattcagcg ggctacagct acgaatattt tggcggcaat gccacttata
      301 tgattattcc cgaaatcgcc attaatttgg gctgcgtatt accgtatcac ggctcttatt
      361 ttgctgcggc gtcgctggca gagcctatgt gctgcattat tggtgcttat catgccaatt
      421 atcacaccac gcaatatgtt tatgagcatc gcatgggcgt caaacctggc ggcaatattg
      481 cactgctggc gtgtgcgggt ccgatgggca ttggcgctat cgattacgcc attaacggtg
      541 gcatacaacc gtcacgggtg gtggtggtcg atatcgacga caaacgcctg gcgcaggtgc
      601 agaagctgct gccggtggat ctggcggcca gtaaaggcat tgagctggtg tatgtgaata
      661 ccaaagggat gagcgatcct atccagatgc tgcgggcgct gacaggagat gtcgggttcg
      721 atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga gatggctgac gaattactgg
      781 cggaagatgg ctgtctgaac ttctttgccg ggctgacgga taaaaacttc aaagtgccgt
      841 ttaatttcta caacgtccat tacaacagca cgcacgtagt cggtacatcc ggcggttcaa
      901 cggacgacat gaaagaggcg attgccctta gcgccacagg acagttacag ccgtcgttta
      961 tggtgaccca tattggtggc ctggatgcgg tgccagaaac cgtgctcaat ctgccggata
     1021 tccctggcgg taaaaaactc atttataacg gcgtgaccat gccgctcact gccattgccg
     1081 attttgccga aaaaggcaaa accgatccgc tgtttaaaga gttggcgcgg ctggttgagg
     1141 aaacgcacgg catctggaat gaacaggccg agaaatatct gctggcacaa tttggcgttg
     1201 atatcgggga ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca
     1261 ggccgcgttg gctgcctggc cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct
     1321 ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct tttcgtggac gagtggttat
     1381 ctgcgaagga gaaattgacc atgcgccgat gctgtggatt ggcgaagagg tgggcaaagg
     1441 cgatgggcca gaagtcgata tcgcggttga tcccattgaa ggcacgcgga tggtggcgat
     1501 ggggcagagc aatgcgctgg cggtcatggc tttcgctcct cgagatagcc tgttacatgc
     1561 gcccgatatg tatatgaaaa agctggttgt taatcgactg gctgccgggg cgattgatct
     1621 gtctttgcct ctggcggaca acttgcgtaa cgtggcgcgg gcgttaggta aaccgctgga
     1681 caagctgcgc atggttacgc tggataaacc gcgtctgagt gccgcgattg aagaagcaac
     1741 acagctgggc gtgaaggttt ttgccctgcc ggatggcgat gtcgccgcca gcgtgctgac
     1801 ctgctggcag gataatcctt acgatgtgat gtacaccatc ggcggtgcgc cagaaggcgt
     1861 gatttctgcc tgtgccgtta aagcgttagg cggggatatg caggccgagc tgattgactt
     1921 ttgtcaggct aaaggggatt acacggaaaa tcggcagatt gcagagcagg agcgcaagcg
     1981 ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg ctcgatgaac tggtgagggg
     2041 gaacgatatc ctctttagcg ctacgggcgt gacgggcggc gagttggtga acggtatcca
     2101 acagacggcg aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg
     2161 taatataata gactccctgc attgatggtg atctatcgga agaccaatgg cgactctgac
     2221 agaagatgat gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac
     2281 tgcgcattct attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa
     2341 cgatgaagag atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga
     2401 cgatattgat gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta
     2461 tgccgatatc acgcactttt cccagtactg gcattacctg aacgaacagg atgaaacgcc
     2521 cggatttgcc gatgatataa cctgggattt tatcagcaat gtcaacagta taactcgcaa
     2581 tgcgacgctc tacgatgcgt taaaagcgat gaagttcgcc gatttcgccg tctggtcaga
     2641 ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa
     2701 ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc tgaacgtgca agcgcagtac
     2761 catgatgaag agatagaacg cgtgcataaa ccgttgctgc gtatgctggc ggccttacag
     2821 acggtaaatc cgcagcggcg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa
     2881 tctaccctaa ccaccttctg ggaatatctc gcgcaacaag acccggagct gcccgctatt
     2941 cagacgctcc cgatggatgg ttttcaccat tacaatagct ggctggacgc gcatcaattg
     3001 cgccccttca aaggcgcacc agagacattt gacgttgcga aactggcgga aaatctgcgc
     3061 caggtggtgg aaggggattg tacgtggccg cagtacgatc gacaaaagca tgatcctgtt
     3121 gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gttgttgctg
     3181 gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt tatcaacgcg
     3241 ccagctgctg cgttgcggga gcgtctggtg gggcgcaagc tggcgggagg ggtatcgctg
     3301 gcagatgcag aagcctttta tgaccgtacc gacgggccga atgttcgccg ggtgctggaa
     3361 gagagtctgc ctgcaaattt gaccttaatg atgaccgcta cgggtgaata tcgtctggtg
     3421 gattaacgtt cctgattaca ccatcttcca gtcgcaccac acgagagaaa tgacgccggg
     3481 taaatgcggc gtcatgactg attgctacca cgctcgtgcc tcgctggcgg cacttctcca
     3541 gccagctttc aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc tcgtccagca
     3601 gtaatagtgg cggagagagt gcttccaggc aggcgacggc gaccatccgt cgctgcgcac
     3661 tatgcagatc caacgggtgc gagttggcag tgtctgctaa ctgacaacat tgcagggcag
     3721 catgggtacg ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga
     3781 tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc ttcctgaaac agtacgccaa
     3841 cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc ctgttgttgc agcataaccg
     3901 taccggctgt ggg
//
LOCUS       DADLTI010000256         3877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLTI010000256 DADLTI010000000
VERSION     DADLTI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.260"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..284)
                     /locus_tag="JN755_004067"
     CDS             complement(<1..284)
                     /locus_tag="JN755_004067"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7382650.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHII"
     gene            393..1319
                     /locus_tag="JN755_004068"
     CDS             393..1319
                     /locus_tag="JN755_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7382651.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1316..2086
                     /locus_tag="JN755_004069"
     CDS             1316..2086
                     /locus_tag="JN755_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7382652.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            2191..2631
                     /locus_tag="JN755_004070"
     CDS             2191..2631
                     /locus_tag="JN755_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7382653.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            2695..>3877
                     /locus_tag="JN755_004071"
     CDS             2695..>3877
                     /locus_tag="JN755_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7382654.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TD"
BASE COUNT          950 a          878 c         1011 g         1038 t
ORIGIN      
        1 ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga aaggcagttc
       61 aggtcggtgt gaatgaccag gttagcaaca ttacggtgaa caaagagttc gcccggctca
      121 agaccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca tagaaagcgc
      181 ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac cagcattttt
      241 gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt taacgacctg
      301 taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta aaccacagat
      361 aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa cttcaacagc
      421 ttaaaaaaaa ctatccaggc ggcgttcagg cgcttcgtgg aatagatttg caggtcgaag
      481 cgggtgattt ttatgcgctt ctcgggccga acggggccgg gaaatcgacc actatcggta
      541 ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt tacgatctcg
      601 agaaagatgt cgtgaacgct aaacgtcagt tgggactggt gccgcaggaa tttaacttca
      661 acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac ggtgtggagc
      721 gcaaagaggc gtacatccgc agcgaaaaat atcttaagca gctcgattta tggggaaaac
      781 gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg attgcccgtg
      841 cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc gtggatattg
      901 aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc accaccatca
      961 ttcttaccac acactacctg gaagaagcag aaatgctgtg cctcaatatc ggcattattc
     1021 aacatggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag ctgaaatcgg
     1081 aaacctttat tctcgatctc gcaccgaaaa gtccgttacc gaagctcgat ggctatcagt
     1141 atcgactggt cgatactgcg acgctggaag ttgaagtgct acgtgagcag gggatcaaca
     1201 gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt aacaaagcta
     1261 accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat cgcgcatgat
     1321 gcatctttac tgggtggcgc taaaaagcat ctgggcgaaa gagatccatc gctttatgcg
     1381 tatctgggtg cagacgctgg tgccgccagt catcaccatg accctttact ttattatctt
     1441 cggtaacctg ataggttcgc gtattggcga tatgcatggc ttcagctata tgcagttcat
     1501 cgtaccgggg ctgatcatga tgtcggtgat caccaacgcc tacgctaacg tcgcgtcatc
     1561 attttttggt gccaagttcc agcgtaatat tgaagagctg ctggtagcgc cggttccgac
     1621 tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt ttgttggcat
     1681 tctggtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt gggtattcgt
     1741 tgccttaacc ctggtgctca cggcggtgct attctccctt gcgggtttgc tgaacggcgt
     1801 gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa cgccactcac
     1861 gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc aagggctgtc
     1921 gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc tcggtataaa
     1981 tgatgtgccg ctggtcacta cctttggcgt actggtggtc tttattgtgg cgttttattt
     2041 gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct atttcctctc
     2101 ctctggattc gggggagagg agttttgacg gctatcaccc tttatcaaca atggtcaggg
     2161 taggctgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac ctgtcacgat
     2221 gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact tcttcagtgc
     2281 cgggccgtga atttctggcg cggattaagc tctaatatgc tcagccgcat gatgtgcgat
     2341 gctctgcatg aagcggactc tggtgagggt gtcatcttct taaccgatat agccggagcg
     2401 ccaccgtatc gcgtggcttc attattaagc cacaaacact cccgttgcga agtgatttct
     2461 ggtgtcacgt taccgttaat tgaacagatg atggcttgcc gtgaaaccat gaccagttca
     2521 gagtttcgtg agtgtattgt cgaactgggt gggccggagg tgagtagtct ttggcaccaa
     2581 caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta acccgcgatt
     2641 ctgatggcgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt tttcatgtat
     2701 aaacaagctg ttattctcct gctgatgctg tttaccgcaa gtgtcagtgc cgcgttacct
     2761 gcccgttata tgcaaaccat cgaaaatgct gcggtctggg cgcaaattgg tgacaagatg
     2821 gtgaccgtgg ggaatattcg ggccggacaa atcattgccg tagagcccac tgccgcaagt
     2881 tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaagggca tctcgagcca
     2941 gttcaggggc gacaaaaagt tgaagacggt ttgggtgacc ttaacaagcc gctgagtaat
     3001 cagaacttaa ttacctggaa agatacgccg gtctacaacg cgccgagtgt gggcagtgcg
     3061 ccatttgggg tactggcgga caatttgcgc tacccgattt tgcataaact gaaagacagg
     3121 ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat cagcgcgctg
     3181 gatgcccaac ccgataatgg cctgccggtg ctaacttatc accatattct gcgcgacgaa
     3241 gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt caataaccaa
     3301 atggcctggc tgcgcgacag gggatacgcg acactgagca tggctcagct ggaaggttac
     3361 gtgaagaata cgatcaatct ccctgcgcga gcggtggtga ttacctttga tgatggcctc
     3421 aagtcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa ggcgacggtg
     3481 tttattgtta cctcacgcat caaacgtcac ccgcagaagt ggaacccaaa atcgctgcaa
     3541 tttatgagcg tttctgagct taacgaaatt cgcgatgttt ttgatttcca gtcacatacc
     3601 cattttttgc atcgggtaga tggttatcgc cgacccatat tactgagccg tagtgagcac
     3661 aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa tccacatgtc
     3721 tggtatcttt cgtatccgtt tggcggcttt aatgacaaag ccgtgaaggc agcaaacgat
     3781 gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg ggataatccg
     3841 ttgttactaa aacgacttta tatcttaaga acggatt
//
LOCUS       DADLTI010000257         3844 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLTI010000257 DADLTI010000000
VERSION     DADLTI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.261"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1058..1498
                     /locus_tag="JN755_004072"
     CDS             1058..1498
                     /locus_tag="JN755_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382655.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1577..3274)
                     /locus_tag="JN755_004073"
     CDS             complement(1577..3274)
                     /locus_tag="JN755_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7382656.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(3594..>3844)
                     /locus_tag="JN755_004074"
     CDS             complement(3594..>3844)
                     /locus_tag="JN755_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7382657.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1005 a          854 c          914 g         1071 t
ORIGIN      
        1 tcctgaaaag caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat
       61 caaagtttag ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct
      121 ctatcattaa aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat
      181 catttaaata tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt
      241 acttgtttta caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg
      301 tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa
      361 cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt
      421 accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga
      481 agatatttat tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa
      541 aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta
      601 tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg
      661 gtgtagcaat gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc
      721 gctacctgga cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga
      781 cgcgttgcgc tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc
      841 cgcagagaac caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca
      901 tattatactg gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc
      961 actgcggagc tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac
     1021 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     1081 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     1141 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     1201 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     1261 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     1321 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     1381 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     1441 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     1501 aaaaccatag atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     1561 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     1621 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     1681 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     1741 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     1801 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     1861 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     1921 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     1981 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     2041 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     2101 ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt
     2161 gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc
     2221 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     2281 tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat
     2341 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     2401 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     2461 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     2521 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     2581 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     2641 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     2701 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     2761 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     2821 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     2881 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     2941 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     3001 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     3061 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     3121 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     3181 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     3241 ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga
     3301 ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg
     3361 aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg ataaataacg
     3421 gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcgacat ttggttcgcg
     3481 attagcgaag aaaaatcatc ataaattggt aaaaaatata acaggtagtt aaaaaacatc
     3541 agtgctgggt aaattgccgg atgacatcag aacgatgcca tccgaacggt ggcttaaccc
     3601 tgacggttga aacgttgcgt tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca
     3661 tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg
     3721 ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt
     3781 tgcagtactg tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc
     3841 ggga
//
LOCUS       DADLTI010000258         3816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLTI010000258 DADLTI010000000
VERSION     DADLTI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.262"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..322
                     /locus_tag="JN755_004075"
     CDS             <1..322
                     /locus_tag="JN755_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382658.1"
                     /translation="TLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQQ
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN755_004076"
     CDS             complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN755_004076"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7382659.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN755_004077"
     CDS             complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN755_004077"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7382660.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(2680..3108)
                     /locus_tag="JN755_004078"
                     /pseudo
     CDS             complement(2680..3108)
                     /locus_tag="JN755_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN755_004079"
     CDS             complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN755_004079"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7382661.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          958 a         1019 c          900 g          939 t
ORIGIN      
        1 aacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc
       61 gatgcaccat gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat
      121 gtacagtcaa caggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca
      181 gcagatcacc aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg
      241 gatattcctc gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg
      301 cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca
      361 attaattgtg aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca
      421 gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc cagaatgcta cgcgcaatat
      481 cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg
      541 ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag
      601 caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca
      661 ttggcgagat atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag
      721 caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc
      781 agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca
      841 cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca
      901 tttagccacc tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc
      961 tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg
     1021 gccactcacg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa
     1081 cggttcggta tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc
     1141 tacaaaatcc tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtaggc
     1201 ttctgcaaac gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg
     1261 ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg
     1321 atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgtggaa
     1381 cagatcttta cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt
     1441 caggcccggt ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag
     1501 ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag
     1561 gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg
     1621 cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg
     1681 ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt tcaatctgca gcacgttgct
     1741 attgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg
     1801 cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc
     1861 aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata
     1921 cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg
     1981 caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaatga cccaaccgaa
     2041 acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc
     2101 gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg
     2161 cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag
     2221 cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc
     2281 tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt
     2341 ataacgatgc agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt
     2401 aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga
     2461 acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt
     2521 ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc
     2581 cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt
     2641 agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc
     2701 ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa
     2761 gattactggc ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac
     2821 cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc
     2881 tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt
     2941 taaccctcca aggctattac ccattgttgc tgttaaaact aaaacctagg gatgactgat
     3001 cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct
     3061 gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca
     3121 tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag
     3181 accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc
     3241 gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat
     3301 cccaggtgcg ccagcaatgc ctctgcgatg gcgctttcac tccccgttcc tacagccatt
     3361 gggcgacgac catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc
     3421 agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg
     3481 gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca
     3541 atcatcgcct gaatatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta
     3601 cggtgcgtag gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa
     3661 cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt
     3721 gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta
     3781 gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DADLTI010000259         3736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLTI010000259 DADLTI010000000
VERSION     DADLTI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.263"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            190..447
                     /locus_tag="JN755_004080"
                     /pseudo
     CDS             190..447
                     /locus_tag="JN755_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN755_004081"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN755_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7382662.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JN755_004082"
     CDS             complement(1599..2351)
                     /locus_tag="JN755_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7382663.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN755_004083"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN755_004083"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7382664.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JN755_004084"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JN755_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..>3736)
                     /locus_tag="JN755_004085"
     CDS             complement(3605..>3736)
                     /locus_tag="JN755_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7382665.1"
                     /translation="AHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          965 a          886 c          889 g          996 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggc
//
LOCUS       DADLTI010000260         3714 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLTI010000260 DADLTI010000000
VERSION     DADLTI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.264"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..375)
                     /gene="panD"
                     /locus_tag="JN755_004086"
     CDS             complement(<1..375)
                     /gene="panD"
                     /locus_tag="JN755_004086"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7382666.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQV"
     gene            649..1551
                     /locus_tag="JN755_004087"
     CDS             649..1551
                     /locus_tag="JN755_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7382667.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(1625..2476)
                     /gene="panC"
                     /locus_tag="JN755_004088"
     CDS             complement(1625..2476)
                     /gene="panC"
                     /locus_tag="JN755_004088"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7382668.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2488..3282)
                     /gene="panB"
                     /locus_tag="JN755_004089"
     CDS             complement(2488..3282)
                     /gene="panB"
                     /locus_tag="JN755_004089"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7382669.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3395..>3714)
                     /locus_tag="JN755_004090"
     CDS             complement(3395..>3714)
                     /locus_tag="JN755_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382670.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
BASE COUNT          893 a          912 c          961 g          948 t
ORIGIN      
        1 aacctgtacc ggaatcgctt tcgcggtacg tttcatttca ttgtcgcctt caaaataggc
       61 gacattgggt cgccaggtgc gagcttcttc atctggcatg gtaacgaagc tggcgatgat
      121 gacaatatcg ccgacgctgg cgcagtgggc cgccgcaccg ttaacagaaa taattctcga
      181 accgcgttct gccgcgatgg cataagtgga gaaacgcttg ccgttggtga cattccagat
      241 atcgatggct tcgttttcga gaataccggc tgcgtcaaga aaatcctggt caatggcgca
      301 agaaccttca tagtgcaggt ccgcatgagt cactttcacg cggtggagtt tgccctgcag
      361 catcgtgcga atcataactt ctacctttct accctgtcgt taacgaagca ggcggtgcct
      421 gctttgagga aattctcacg cagtattgcc cgatttttga tgagtgtcta ctcatctgat
      481 ggcatttgcg tcagcagttt gcgtaccgcg ccgaagcgtg ccatgtaacg cctgtaacga
      541 attgatattt ttatgaatgc tgctcggtaa acaataaaga ggtggctgac gatagcacgc
      601 cttgactgga tactgccttc acgcaaggcc acacggaaga cagggaagat ggatgcaccg
      661 agtaccacac cgcatgacgc ggtatttaaa caatttttaa tgcatgcgga gacggctcgc
      721 gactttctgg agatacattt gccagtggaa ttacgcgaac tttgtgatct caacacgctt
      781 catttagagt cggggagttt cattgaagag agcctgaaag gtcacagcac ggacgtgctc
      841 tattccgtgc aaatgcaggg caatcccggt tatctgcatg ttgtgattga acaccaaagc
      901 aagccggata agaaaatggc ctttcgcatg atgcgttatt ctattgctgc catgcaccgg
      961 catctggagg ctggccacga taagctgccg ctggtggtgc cgatactgtt ttatcagggc
     1021 gaggccacac cttatccgct ctcaatgtgc tggtttgata tgttttactc gccggagctg
     1081 gcgcgacgcg tctataacag tcctttcccg ctggtggata tcaccatcac accggatgac
     1141 gaaatcatgc aacatcggcg gattgcgatt ctcgaactac tgcaaaaaca tattcgccag
     1201 cgcgacttaa tgttattgct ggagcaactg gtcacgctga ttgacgaagg gtacactagc
     1261 ggaagtcagt tagttgccat gcaaaactat atgctgcaac gcggtcatac tgaacaagcg
     1321 gatttgtttt atggtgtgct gagagacagg gaaacgggag ggaagtctat gatgacgctg
     1381 gcgcagtggt ttgaagagaa agggattgag aaggggattc cgcagggaag acaggaggaa
     1441 aggcaagaat tcgccctgcg tcttctaagt aaaggaatgt ctcgggaaga cgttgcagag
     1501 atggcaaatt tacctcttgc tgagattgat aaggtaatta accttattta agttacctgt
     1561 gttatgacag atgacgtggg gtaaattaat aactggcgcc atcagccgta gcgccagtta
     1621 agtattacgc cagctcgacc attttgttgt cgatcaggcg agcatcgcca agccaggcgg
     1681 ctaccagaat tactgcccgt ttgctggttt cagaaacttc cagcaatgtg tcggcatcgc
     1741 gaatctgaat atcatcggcg cggaagcctt tttcattcag ttcttgcccc gcaatggtaa
     1801 taatttcatc gagatcccgt tccccagcct gcaatttgtc agcaatcgaa cttaaaactt
     1861 tgtacagacc aggcgcaatt ttgcgttgtt ccgccgtcag ataaccgtta cgggaactta
     1921 gcgccagacc gtctttggcg cgcataattg gcacaccgac aatctcaata tcgaagccca
     1981 tatcggcaac cattttgcgg atcaacgcca gttgctgaaa atctttttca ccgaagcagg
     2041 cgatgtccgg ctggaccagg ttgaacagcg tgctgacaat agtcgaaacg ccgcgaaaat
     2101 gtcccggacg gctggcacct tccagcatgg ttgaaaggcc aggaacgtca acgtaagtgt
     2161 gggtttcagt accgttcggg tagatctctt ttaccgaagg ggcgaaaact aaatccactt
     2221 tacgtttgtt tagcttctcg cagtcctcct gcaaggtccg tggataacgt gccagatctt
     2281 ccgggcggtc gaactgcatc gggttaacga aaatactgac gacgaccaca tcggcgcggg
     2341 ctttggcttc gtcgaccagc ttcatatggc cattgtgcag attacccata gtaggcacca
     2401 gcgccacgcg cttgccttcc atacgcaggc ggcgaatttg ctgacgcagc agcggcaggg
     2461 tttcgataat taacacaacg tgactcctta atggaaactg tgttcttcgc ccggataaac
     2521 gccggactcc acttcagcca tatactgccg cacagccgcg cggatgtcgc ccgtttcggc
     2581 gaggaaattt ttagcaaatt taggaatgtg accgccggta ataccaaagg cgtcgtgcat
     2641 cacgaggatt tgcccgtcag tgacgttgcc tgcaccaata ccaataaccg ggatcgccag
     2701 tgcttcggta atacgttttg ccagctcaac cggcacgcat tccagcacca gcagctgtgc
     2761 ccccgcggct tctaaggcta atgcatcgct gagtagtcga tcgctcgctt cattgccgcg
     2821 cccctgtact ttgtagccac cgaaaatatt cactgactgt ggggttaaac ctaagtgacc
     2881 acatacagga acggcacgtt cggtcagcat ttttacggtt tcgaccagcc actcaccgcc
     2941 ctcaattttg accatattgg caccggcacg cataaccgtt gcggcgtttt caaaggcttg
     3001 ttccggcgtg gcatacgcca taaacggcag gtcagccaat agcaggcagt ttggtgcgcc
     3061 gcgacgtacg gcggcagtgt ggtaggcgat atcagcaacg gttactggca gagtggaatc
     3121 gtggccctga acggtcatgc ccagcgagtc gcctaccagc atgacgttaa gcccttcatc
     3181 agcaaatagt ttcgcgaagc tgtagtcgta agcggtgatg gtcgcgaaac gctttttatc
     3241 ctgcttatat ttctgaagtg aagcaatggt ggtcggtttc ataacgtatc ctgataaatg
     3301 atgtcgtgtt gcctggcatt ttatcagtca cattggtggg ggcaatgatt tatcaattac
     3361 cgcaccgcca gatgatggcg gtgcggtgga cttattaagg gtaggttacc tggaaagtac
     3421 tggtggcttt aaactcacca gcttctattg gtatagttcc atcctgttgt agcgtggcct
     3481 ggaaatggag agggtatgtt atacctgtat cttgatccgg gtaaactcct cctgttgtat
     3541 cgtctttgcc tctcgggtcg taatctttat aaacagaatt tgaatcatta ggtttcaatg
     3601 tcattagagg atttttacta gttgctaaac cttctatgag tacaccgact ccttttgctg
     3661 cgtcattacc tgtgagagta ttaccaagga gttgcccgtt ttcagtacca acct
//
LOCUS       DADLTI010000261         3703 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLTI010000261 DADLTI010000000
VERSION     DADLTI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.265"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..975
                     /locus_tag="JN755_004091"
     CDS             <1..975
                     /locus_tag="JN755_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7382671.1"
                     /translation="YPNKYSYVWKPTSRESVTLTAIKDGLAALWKKYEETVNNRDRAM
                     TFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVPADSIKPEHIRRYMDKRG
                     EQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKNRERYVTDKEYQAVLSV
                     APLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKTGKKQIKAWSPRLQAAI
                     EKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKISGILTDFTFHDLKAKGIS
                     DYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENIPRKYSK"
     gene            complement(1028..1117)
                     /locus_tag="JN755_004092"
     tRNA            complement(1028..1117)
                     /locus_tag="JN755_004092"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Ser,seq:cga)
     gene            1211..2008
                     /gene="mtfA"
                     /locus_tag="JN755_004093"
     CDS             1211..2008
                     /gene="mtfA"
                     /locus_tag="JN755_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7382672.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2109..2184
                     /locus_tag="JN755_004094"
     tRNA            2109..2184
                     /locus_tag="JN755_004094"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2142..2144,aa:Asn,seq:gtt)
     gene            2346..3608
                     /locus_tag="JN755_004095"
     CDS             2346..3608
                     /locus_tag="JN755_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7382673.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSPNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1023 a          860 c          885 g          935 t
ORIGIN      
        1 tacccaaata aatatagtta tgtatggaaa ccaacatcca gagaatctgt aacactaacc
       61 gccatcaagg atggtttagc tgctttatgg aaaaagtatg aggaaactgt aaataatcgc
      121 gatcgtgcaa tgacattcgg tcgcttgtgg gaaaaattcc tcgccagcgc ctattacagt
      181 gacctcagtc caagaacaca aaaagattat ctgcaacatc aaaaaaagtt gcttgccgta
      241 ttcggtaagg tgccggcaga ttccataaaa ccagaacaca tccgtcgata catggacaag
      301 agaggggaac agagtaaaac gcaagccaac catgaaaaaa gcagtatgtc ccgcgtttac
      361 agttgggggt atgagcgagg gtacgtgaag gctaacccat gtgcaggtgt aagtaaattc
      421 aaggccaaaa accgcgaacg atatgtaacc gacaaagaat accaggcagt attaagcgtt
      481 gcacctcttc ctgtttttat cgcaatggaa attgcctatc tgtgtgcagc gagggtttcc
      541 gatgtgttat cgctgaaatg ggagcagatt ggaaacgacg ggatctttat ccagcaaggg
      601 aaaacaggaa aaaaacagat aaaagcatgg agtccacgat tacaggcggc gatcgaaaaa
      661 gcaaaacagt taccaacatc cgcctatgta atcagcaatc aatacggcaa ccgatatatg
      721 tacaaaggct ttaacgaaat gtgggtagaa gcaagaaatc gcgcaggcaa aatttcaggt
      781 attttaaccg acttcacctt tcatgatctg aaggcgaaag gaatttcaga ctatgaagga
      841 agcagtcggg ataagcaact tttctctggt cacaaaaccg aggggcaagt gctaatctat
      901 gacaggaagg ttaaagtttc accgacactt gatgtcccgt tacctgaaaa tattccaaga
      961 aaatattcca agtaattcca agtgtgattt ttgtcactga cttaatgatg tataagtgat
     1021 tgaattttgg cggagagagg gggatttgaa cccccggtgg agttgccccc actccggttt
     1081 tcgagaccgg tccgttcagc cgctccggca tctctccgct cagatggttg ccatgatgcc
     1141 aggaaatttg gcattttaac agtccctgtc cgtgcaattt tgttcaagtg acgagtttgc
     1201 gagcaaaacg atgattaagt ggccctggaa agtacaagaa tcagcacatc aaactgccct
     1261 tccctggcag gaagcactat cgatccccct tttaacgtgt ctgacagaac aggaacaaag
     1321 caaattggtc gctcttgccg aacgtttttt acagcaaaaa cggcttgttc ctttacaggg
     1381 ctttgagctg aattcattaa gaagctgccg gatagcactt ctattttgcc tgcccgttct
     1441 ggagttagga ctggaatggc tggatggttt tcatgaagtc ttaatttatc ctgcgccatt
     1501 tgtggtcgat gatgaatggg aagacgatat cggtctggtg cataaccaac gtattgttca
     1561 gtcaggtcag agctggcagc aagggcctat cgttttgaac tggttggata tacaagattc
     1621 ttttgatgct tctggtttta acctgattat tcatgaagtc gctcataagc tggacacccg
     1681 taacggcgat cgcgccagcg gagttccctt tatttcgttg cgtgaggttg ctggctggga
     1741 acacgatctt catgctgcaa tgaacaacat tcaggaagaa atcgaattag ttggtgagaa
     1801 tgcggcgagc attgatgctt atgctgccag tgatcctgct gaatgttttg ccgtactttc
     1861 tgaatatttc tttagcgccc cagaactttt tgctcctcgt ttcccttcat tgtggcaacg
     1921 tttctgtcaa ttttatcaac aagatccttt gcagagactg catcacgcta atgatacaga
     1981 ctcgttttcg gcgacgaatg ttcattaatt aacaactttg cagattaatt aaccaattga
     2041 aatggcttat gaaatttagt gttgacagac aaggtaccgc taagtaatat gcgccccgtt
     2101 ctcacgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg
     2161 gttcgagtcc agtcagagga gccaattttc tgttttcatg catccttgcg aatctttatc
     2221 tgatgttgat tcaacaggtt agtgtgaaaa ctcttctcgg tgcattttga ttttacccta
     2281 cgcatcggga aaaattggtg gtcaaatctg ggatcaggtt agttcgataa tggagtgacc
     2341 cccatatatc ccttaccgac gcaaaaatcc gcaccctcaa gccttctgat aaacccttta
     2401 aagtctccga ttctcacggt ctgtatctgc tggtcaagcc gggtggctcc cgccactggt
     2461 atctcaaata ccgtattagc ggtaaagaat cccgcattgc gctgggtgcc tatccagcca
     2521 tctccctgtc tgatgcgcga cagcaacgtg aaggtatccg taaaatgctg gcgctgaata
     2581 tcaacccggt acagcagcgg gctgctgaac gtggctcacg aacaccggag aaagttttta
     2641 aaaacgtggc gctggcgtgg cataaaagta acaggaaatg gtcgccgaac accgccgacc
     2701 gtctgcttgc cagcctgaac aatcacatct ttccggtcat cgggaaccta cctgtatcag
     2761 aacttaaacc ccgtcatttc attgacctgc tgaaagggat cgaggaaaaa ggtctgctgg
     2821 aggttgcgtc ccgcacacgg cagcacctga gtaacataat gcgccatgcg gtccatctgg
     2881 agttaatcga tacgaaccct gcagcaaacc ttggcggcgt gaccacacct cctgtcagac
     2941 ggcactatcc tgccctgccg ctggagcggc tgcctgaact gcttgaacgt attggggcat
     3001 atcatcaggg ccgtgaactg acccggcatg ccgttctgct gatgctgcat gtgttcattc
     3061 gctccagtga actgcgtttc gcccgctggt cagagattga tttcacaaac cgagtctgga
     3121 cgatacccgc gacgcgagaa cccattattg gcgtgcgtta ttccggccgc ggggcaaaaa
     3181 tgcgaatgcc gcatatcgtc cccctctcag aacagtccat cgccattctg aaacagatta
     3241 aggatatcac cggtaataat gaactgatct tccccggcga ccataacccg tataagccaa
     3301 tgtgtgaaaa cacggtcaat aaggcactgc gggtgatggg ttacgacacg aaaaaggata
     3361 tctgcggtca cggcttccgg gcaatggcat gcagtgcgct gatggaatcg ggtttatggg
     3421 caaaggacgc agtagaacgc cagatgagtc atcaggagcg caataccgtg cgcatggctt
     3481 atattcataa ggcagagcac ctggaagccc gcaaagcgat gatgcagtgg tggtcggatt
     3541 atctggaagc atgccgagaa tcttatgcac cgccttatac aattggtaaa aataagttta
     3601 tcccatagta tactaacgaa tcccacactc tggcaaatta ccaccacctc tacaggaggt
     3661 ggttctatat agacttttat caactttatg acgaaaaaca cct
//
LOCUS       DADLTI010000262         3687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLTI010000262 DADLTI010000000
VERSION     DADLTI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.266"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..222
                     /locus_tag="JN755_004096"
     CDS             <1..222
                     /locus_tag="JN755_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7382674.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..477
                     /gene="hha"
                     /locus_tag="JN755_004097"
     CDS             268..477
                     /gene="hha"
                     /locus_tag="JN755_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7382675.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            516..1106
                     /locus_tag="JN755_004098"
     CDS             516..1106
                     /locus_tag="JN755_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7382676.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1346..1606
                     /locus_tag="JN755_004099"
     CDS             1346..1606
                     /locus_tag="JN755_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7382677.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1830..1904
                     /locus_tag="JN755_004100"
     CDS             1830..1904
                     /locus_tag="JN755_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7382678.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1897..2754
                     /gene="repA"
                     /locus_tag="JN755_004101"
     CDS             1897..2754
                     /gene="repA"
                     /locus_tag="JN755_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7382679.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="JN755_004102"
     CDS             3454..3645
                     /locus_tag="JN755_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382680.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          992 a          901 c          932 g          862 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta
      421 atcaccggaa agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa
      481 ttcagcaata attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca
      541 ttgttctgat tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt
      601 ctcctttgct gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac
      661 tgtccagcgg tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt
      721 ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac
      781 gggtcgcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc
      841 agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt
      901 tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac
      961 gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc
     1021 acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc
     1081 agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     1141 acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt
     1201 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     1261 ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     1321 cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt
     1381 gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc
     1441 cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct
     1501 gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc agttcgttga
     1561 gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt
     1621 ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg
     1681 tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa
     1741 tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa
     1801 caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     1861 gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat
     1921 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt
     1981 ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc
     2041 attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt
     2101 cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac
     2161 cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     2221 gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga
     2281 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     2341 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
     2401 cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg
     2461 ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac
     2521 cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg
     2581 cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg
     2641 cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc
     2701 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
     2761 cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc
     2821 acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg
     2881 tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct tatccacatt
     2941 aaactgcaag ggacttcacc gtaaggttac aaccgttcac gtcataaagc gccagccgtc
     3001 aaccctacag ggtgcaatgt atcttttaaa cacctgtgta tatctccttt aaactactta
     3061 attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc
     3121 acctcccacc gcaagcggcg ggccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac
     3241 tgaaaagcgc cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgagta cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatgactg aaaacgggta tggcttagca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DADLTI010000263         3665 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLTI010000263 DADLTI010000000
VERSION     DADLTI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.267"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(2..298)
                     /locus_tag="JN755_004103"
     CDS             complement(2..298)
                     /locus_tag="JN755_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7382681.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="JN755_004104"
     CDS             1050..1598
                     /locus_tag="JN755_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7382682.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1138 a          692 c          820 g         1015 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataaccctt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattctatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 actatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg gatcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcacgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt aacgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgga
     2341 agagagggaa cggaagagag ggaacgataa gaccagaggt agtgacctgt taaacaggct
     2401 gtttcaaagt ctcttaaaag tctgttaaga gggctaggtg aacaatcctg ccattttgtc
     2461 aagtgttttg tctttagcaa aaagtgctaa acttaacaaa aagcactaaa tgctaaagag
     2521 acgattaaga aatatactaa gaggggcgct tcgcttgggg tcactatgtt ctttctctcc
     2581 cgttaagagg atttacaggg acatttcaaa gccctgataa ggtaagcccg atgataacag
     2641 aaacagaggg caaaaccact tatcaacact gtgaacgaat aacgtaaagg gctggttaag
     2701 aaagtcggtt tagggattgg taacaatacc aattagaagg gagcttccct ttctggggtg
     2761 tcgctctatg ggtgaagtta acctttgaag ttctctgtga agaaactgaa cagctattat
     2821 attggctaat tacaaccctt tgaaagataa gcatttttat aagttactgt taaggttgca
     2881 gagttgtctg tgaagagact tgtcagttct ctatattggg tggttacaac tcattgaaaa
     2941 ataagagctt ttagaggtta ctgtcagaga ggtgaatact tgatagatac agtcttccgg
     3001 ttctgtcagg ctatttttta acagatagca caaaatgcta aagatgcccc tatgccatct
     3061 cgtacagggg ggttttgaag ggagtttaac gggggtctgt gtggtcatta tccttaaggt
     3121 caagcgtcca tgtgcgaccg aacagtttaa agacaacaag cactctgacg aggacgtaac
     3181 gaagtagcag atctgcaata agtccaatac ctccaccagc ccatacatca ggtgacccca
     3241 gctcaggagg gagatttacc attagttttc cttgtaaggc gctctcaatc gcattctgac
     3301 acaaaaaagt acctgacctt atgagttgac ctgaagaggt catttaactc gttacagagc
     3361 gattgaagag gtctgtgtgc aatgcaataa ccatgtgacc agatacatag ccacgcctca
     3421 tattttactc tccccataaa cccattacac cgcagatgtt acgaaattgc taaaaatact
     3481 tggtattagt ctttgaaaat gctagcattg tatatataag ttaacagaat aataacatgt
     3541 tatgaggggc agaatatgaa tgagagacgc agaatttaga gcgatgcttc aggcatcaag
     3601 agaacgcaac aagcacaact cctacgctta cacaaacaac cccaccagct acgaagtccc
     3661 aacct
//
LOCUS       DADLTI010000264         3591 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLTI010000264 DADLTI010000000
VERSION     DADLTI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.268"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(617..2326)
                     /locus_tag="JN755_004105"
     CDS             complement(617..2326)
                     /locus_tag="JN755_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7382683.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2758..3477)
                     /locus_tag="JN755_004106"
     CDS             complement(2758..3477)
                     /locus_tag="JN755_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7382684.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1067 a          721 c          641 g         1162 t
ORIGIN      
        1 gatacaactg gtctggtata tacagtttct ttgagtgcgg ttacgtccac agaggtaacg
       61 gtaaaagtaa ccccttgtcg ggaccactcc gaccgcgcac agaggcattt tcatcgctga
      121 caggccgcat tgcctcgcag ggctggagga ggatcggtga tatccggacg accgtaccgg
      181 cttacgccgg gcttcggcgg ttttactcct ctgccatgtg caacaacgga gtgccgcctt
      241 acatgccgct ggcgcggcat atcctggtaa catttttgtt gataccacct tccggcctta
      301 tcctgaccag tgggctttcc tggcatcgat gataccgatg aacgcgcatg atattgagcc
      361 gacgatatta cgggcaactg gaaatactca ctctctggat gtgaccttta tccatgaaga
      421 agatttggcc acaccatgga aacctgaaca atcatcagtt tacgcccatg taaaccccta
      481 cgggatattt gaactcgata tggaaacaag gttgccaatc gaggtggttg cctgaaagtt
      541 ggcagttacc cggtcactac accttgttgg agtgaacata tcaaaaacag gtaattacac
      601 aatttaaatt acagtctcat aaaaaatgat taaatctatt tattatttca ataaacgttt
      661 ttttcatctt ctttgaaaat tgttctgcta tagtatccgg ttccagattg ttctctttta
      721 ttattctaca atactctaat attgtttctt tgtctctatt tactacagat acaaatacac
      781 caggaatacc atctttatta gttgcggaca gcatttcgac caatcgtctt gtggtaaaac
      841 tatagttttt tagaatatta caaaaggcac gtattgtttc ggtatggcca ttataaaatg
      901 ccataaaaag tcctgatgta ccgttgggat tttttatcca taataattca tctatatatt
      961 cctggtgagg ggggagtatt tttagtatat ctccatacgc cataatggcc tttgtgtgcc
     1021 cattttgaag tgccataaac aacccatgct ttactttcaa taattcttta atttgttcac
     1081 tactaagcat tttcttatta agcagacttc cgaacatacg aatactatct gcatgtccat
     1141 tctgcaatgc aaaaaataac ccaggtaaat catggctgtc tttcgcagct aatagatata
     1201 atctcttatc ggaagttaac ggggttttaa agactatttc cccataggct tttattgtgt
     1261 ctacatgtcc gttcataaaa gacatgcata aacccggcac attgtttgcc cctaccgcag
     1321 caagcaatgt aagtattgtt tcttgactaa gattgcacat atttaatatc ttaccatata
     1381 cttgaataat gtctgaatat ccgttttgaa gtgctatata aaatgcagga gtaccatcag
     1441 agcttctcgt ggccagaagg ttcaccagtt tagattgaga aatttcacca tttttgtatt
     1501 tttccatcag tataaataat acttctgtca gattctttgt tatcgcatga tatataactc
     1561 tggaattgat caattcatct ggtaaaagtt tcactgtgtt tgttagaggg gaattatgcc
     1621 tatcttcaaa tattacctgc ccgggttgct ccagaccata aattttccaa taaaaatcat
     1681 caaaaaaatc cttagcggat aattgactca gttcgaaatg gctatcacaa gtaaatacag
     1741 tacgtttatg agtaacagtt cgattaggat cgaaaaactg aacaacccat ttttcacttt
     1801 cttgtacgcg ctttattttc aaccctaacg ccatttggtg gtttaatgtg catgctgata
     1861 atgttctgac aaagtctcca ttttttttca tgttataaaa aaaattagta ataactgttc
     1921 cccacttttt attttccaca aaataaatat aagctggtga ttttgaaaaa tcttccgttt
     1981 tttccaaaca tacatttttt gcaatagcat caggggtgct aagaagctga tagtttacct
     2041 tcccatttgt ttccataaaa ttcatttccc aataggaagc taggtgacgg caaactattt
     2101 gtcttccata tatatctttt gccactccat ttatatttac cttcacattt ctcttttctg
     2161 aaaaataagg tgctgtttta ttcttaaact gaattagttc actggagttt gcgaccctca
     2221 taatagaact tgttattgtt ctattatctc tgttaacaga catatctgtt gataacgaat
     2281 tttttgttga aaatatacta gatgaaaatg ttttatttaa cggcatagaa gacaaccata
     2341 gagatgttat aatactatta gattgcagtt actaatagta aaagcctcct tacaaggagg
     2401 tagcttattc agaatgctcc tgtaagatag cgagctcgtc aacgatacag atgatctgat
     2461 tatattgtgc aaaaggagaa caggaaaata ggcactggaa aagatgaagg cctgatggga
     2521 gcattaacta atgcaaaatc cgacaggtta atatcccgaa aagcaatcaa acgactcaaa
     2581 caggagatgc acgctgaagt ggtggacaac tgttcggatt tcagatgagt agctcagcag
     2641 tttatgagcc gaatgctctg tcacgggcag gaatcaggct gcatacgcag tgccaaatct
     2701 aatcgtagtt attctaacca gttttcaagc tacaaggtgg tgtagctggc atcttctcta
     2761 ctctatcatc aaacgataaa atggttcctc tcgtaacatt tgctcttgcc tttcacttcc
     2821 atctcgatcg cttcgtagtt cattacgata gctgacatat ttccagtctt caggtctaac
     2881 atctgacgcc ggatactccc cgggagcgat tttgcttctt aatagattag actcgagaca
     2941 cattataaat tgtcgtattt tatgaagtaa taaagcacta tattgcgagc attcctgatc
     3001 tgattttaca tatagcgtaa actgagctcc tatccccaca cgagattgct gagaaactcg
     3061 attcatatca gttatcttcc atttatctat aggactgtcc tctgaaaata ataaaccaga
     3121 caaaatttgg aatgccagag gaacttgctc cctagctata ctaatatgaa acttgtcccc
     3181 aacaaaatcc cccttactct gaggattttc acgacatgca tgaataaaca catctccact
     3241 atggttcaca atctggaatc cttggtacat gacagaaaaa aattgacata tatcagagta
     3301 agttggcacg ttcgatgcta ctttgaaata gtacgctggg tattgccgta tttggttgta
     3361 caatatattg aagttatttt tcaatctttc attcgcagaa agcatctgcg gtatttcaga
     3421 cctcacaaca ttcaaagaat ctaaatttag ttttagacag ggctttttta tgggcataga
     3481 aaacgtcctc tataaaatag atatattatc ttatattgat tgtttatcat cttttataaa
     3541 acgatacatt atatgtaatg tatgtaagcg taagggtctt ctccgatcat g
//
LOCUS       DADLTI010000265         3566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLTI010000265 DADLTI010000000
VERSION     DADLTI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.269"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            289..807
                     /locus_tag="JN755_004107"
     CDS             289..807
                     /locus_tag="JN755_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7382685.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="JN755_004108"
     CDS             814..1047
                     /locus_tag="JN755_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeD"
                     /protein_id="HAY7382686.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="JN755_004109"
     CDS             complement(1349..1816)
                     /locus_tag="JN755_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382687.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2144..2485)
                     /locus_tag="JN755_004110"
     CDS             complement(2144..2485)
                     /locus_tag="JN755_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382688.1"
                     /translation="MRRWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPS
                     CALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAP
                     YPVTIVLSPTR"
     gene            complement(2886..3077)
                     /locus_tag="JN755_004111"
     CDS             complement(2886..3077)
                     /locus_tag="JN755_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7382689.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3464
                     /locus_tag="JN755_004112"
                     /pseudo
     CDS             3142..3464
                     /locus_tag="JN755_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          858 a          798 c         1061 g          849 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgcgca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgctgggaa ggggcaacgg tgcttcaggg
     3541 taatccggaa gaagtccggg aactca
//
LOCUS       DADLTI010000266         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLTI010000266 DADLTI010000000
VERSION     DADLTI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.270"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JN755_004113"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JN755_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7382690.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPD"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JN755_004114"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JN755_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7382691.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JN755_004115"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JN755_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7382692.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          862 a          912 c          921 g          822 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaatcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaatgga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADLTI010000267         3513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLTI010000267 DADLTI010000000
VERSION     DADLTI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.271"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..454
                     /locus_tag="JN755_004116"
                     /pseudo
     CDS             1..454
                     /locus_tag="JN755_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JN755_004117"
     CDS             514..621
                     /locus_tag="JN755_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7382693.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JN755_004118"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JN755_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7382694.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLSAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JN755_004119"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JN755_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JN755_004120"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JN755_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..>3513)
                     /locus_tag="JN755_004121"
     CDS             complement(3422..>3513)
                     /locus_tag="JN755_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7382695.1"
                     /translation="QQLNASIAVEGLDAKKVAADYLKQKGWAK"
BASE COUNT          880 a         1082 c          874 g          677 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcagacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gca
//
LOCUS       DADLTI010000268         3475 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLTI010000268 DADLTI010000000
VERSION     DADLTI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.272"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..1985
                     /locus_tag="JN755_004122"
                     /pseudo
     CDS             1..1985
                     /locus_tag="JN755_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1991..2704
                     /gene="ybiX"
                     /locus_tag="JN755_004123"
     CDS             1991..2704
                     /gene="ybiX"
                     /locus_tag="JN755_004123"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7382696.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="JN755_004124"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="JN755_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7382697.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..>3475
                     /locus_tag="JN755_004125"
     CDS             3309..>3475
                     /locus_tag="JN755_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382698.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLA"
BASE COUNT          896 a          923 c          873 g          783 t
ORIGIN      
        1 gcgtgggtgc gttttttgcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg tattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggcg cagcgcaccg acaggctcga tcaatatgat cagcaagcag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gatactaccg cggtgcgcct gaatgtgatg ggcgaaaaaa
      361 cccacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtct
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc actcagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gagcggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctagtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga gacgcagact aactatggcg ttaatccggt gacgttaccg gcggtaaata
      961 tttatcatcc tgacagcaga attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg ccttcgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cagtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggcaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagtgg taacagtgcc aaccgcaccg
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcgctgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg gacttactcg caatacggta agaaacgcgt cgaaggctat gagatatccg
     1561 tggcagggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag aaaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccaatatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atacataaag gttcagacgg cgcggtggga acgccagcat
     1801 ttacagaagg ttactgggtc gcagatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcgagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga acttgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc ccattttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggagc acaaggtgca caagttaaga acaatcagca
     2161 agtagacacc cgcagcgcgt tatacgccgc actgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgca ttgttctttg ccgctgcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt ccatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga cacctttgga caacatcggg taaaactccc
     2461 ggcaggtgat ctggtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactgg acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccgctt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atatcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgtaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctgtcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctggc
//
LOCUS       DADLTI010000269         3406 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLTI010000269 DADLTI010000000
VERSION     DADLTI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.273"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            358..579
                     /locus_tag="JN755_004126"
     CDS             358..579
                     /locus_tag="JN755_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY7382699.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            598..>3406
                     /locus_tag="JN755_004127"
     CDS             598..>3406
                     /locus_tag="JN755_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7382700.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFR"
BASE COUNT          773 a          847 c         1110 g          676 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggg gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggct gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggggcac ggccgtcgtg
      781 ctgaccggtt ttgtggcggg tgtggcgatg gtgtacaccg gcgtgacaga aaaggtcagc
      841 gaagcctgtt cagccctcgc aaatatgctt ttcccgcctc agattgaagg ttacatcctc
      901 accggctctg gtgacacatg gatcaacagc aaacctgccg cgcgggcagc ggcgactgcg
      961 gcatcgcggc aggacataga ggcgcaggaa gcgcaggcga aggcagaaca ggaagaagcg
     1021 cagcgtcagg aggatgccag aacgttccgg gatgtggccg gggaatatct ggaaatggcg
     1081 ggggccatag ccctggcggt taacccggtg acagggcctg tgctgatgtc ccgggctctg
     1141 cacgaccagc tcagtacgga agaagggcgt tctgaaatgg tggatggcgt gacacatttt
     1201 gtgtcggaac tatggcagcc cactgtggcc agtgcggccc cgggctccac cccgacccct
     1261 gacgataaaa tcgactgcca taaacatcct tcctcactga cgcagtttct cgcacaaaaa
     1321 aaagccgcct ttctggatga ccccctggga acggccctta acctgctgaa tccgggcggt
     1381 atgctggaaa ccgcctttca gggagtgaat gcggttattg gcagcatcag taatctgttc
     1441 aaaggcgatg acgagccgcc cgcagcggaa tatatcgcgg aaggcgcgcg ggatgtgcgt
     1501 atcaacagcc agccggcgac acgcagcggg gcacgctgta cctgcgaggc cagggtggtg
     1561 aatgagccgg ggaacggggc gtttgtgtca ccggatgtgc gcattggcgg tccgttactg
     1621 gtggtcaggg atatcagaag cggcaggtcg cagataaccc tggtggcaac cgtggcgctg
     1681 atgtttcttc gcccgggcaa aatgctttct aaaatcgcct gttttgctgc cagtgtcggg
     1741 atgagcatgc tgacgcagaa ggccaccagt gcccttccgc atccggtcaa tgccgccacc
     1801 ggggccaaat acctggccga tgacgatgac ttcgatttca gtctgccggg ccactttccg
     1861 ctggactggc agcgcgtgta cagcagtcgt gacgaacgta cggaaggaat gtttggtcag
     1921 ggctggagtg taatgtatga agtcagcctc gtgtgcacac cgggtagcgc agacgaaaac
     1981 tgcatgacct ttgtgtcggg catggggcga cgcctggata tggaagccgt actgccgggg
     2041 ggcgggtttt acagccccgg tgaagggctg gcggtaaggc gtggtgagca ggggcactgg
     2101 cttatcagca gcgatgacgg gcagtgtttt ctgtttgagg ctgacccgca ccacccgcag
     2161 cgccagcggc tgaaaatgct gggcgaccgt aacagcaact gtctgaatct gtactacgac
     2221 gaccggggcc gcattgtgga aatcagcggc gaacagcagc gtccgtgcat ccgtctgtat
     2281 tacgaactgg cggcgcatcc ccggcgggtg acacagattt atcagcattt ccctgagact
     2341 gcacctttac tgctgcgtcg ttacagctat gacgaggccg gccacctgaa cggcgtgtac
     2401 gacagcacgg ggcacctgct gcgtgagttt gcttacgacg aaaaccactg catgacgctg
     2461 caccgccagc cgggcggcga agggtattat taccagtggg gctggtatga aggtccggat
     2521 gacgcggcat ggcgggtgac ggggcaccac acggacagcg gtgaacagta ccggctggcg
     2581 tgggacctgg cgcagcgcag gctgtgcgtg acggacggac tggggcgcac acgttatcac
     2641 cagtgggatg cgcagaatca ggtgacggcg taccaggacg aagccggaca ggtgacgaca
     2701 ttccgctgga gcgacgaaga acgactgttg ctggggatga ccgaccctca gggcggcaaa
     2761 tggcgctatg tgtatgacct gcaggggcat atcacggaga cgcacgaccc gctggggcgg
     2821 gtggagcagg cgcagtggca cccggtgtgg caccagccgg aaacggaggt ggacgcggcg
     2881 ggaaacacct ggcgctgtgt gtacgatgaa cgcggcaacc ttcttgccgt gaccgacccg
     2941 ctgcagcaga acacccggta tcagtatgac caccacggcc aggtggtgca gataacagat
     3001 gcgcgcgggg ggaataaata cctccagtgg aatgaagacg ggcagctcat gcggcacacg
     3061 gactgttccg gctcgcagac ggcgtggttc tacgacgaac gcacgaggct tatcagaatg
     3121 acggatgcgc agagccacag cacgcgctac gggtatgacg acagcggtca tctgacggag
     3181 gtgatactgg cggacggccg cacggtgaat taccagtcag acgcggcggg caggctggta
     3241 aagtacacca gcccgatggg gaggataacg cgctggcagc gggacgggca gggccgggtg
     3301 cgcagccgga cggacgcgat ggggcgcagg acggcattcg ggtacgacgc ttacgggcgt
     3361 ctggtcacgc tcacgaacga gaacggagaa cgctactggt tccgcc
//
LOCUS       DADLTI010000270         3392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLTI010000270 DADLTI010000000
VERSION     DADLTI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.274"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..192)
                     /locus_tag="JN755_004128"
     CDS             complement(<1..192)
                     /locus_tag="JN755_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382701.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQP"
     gene            complement(328..2169)
                     /locus_tag="JN755_004129"
     CDS             complement(328..2169)
                     /locus_tag="JN755_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7382702.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2169..3389)
                     /locus_tag="JN755_004130"
     CDS             complement(2169..3389)
                     /locus_tag="JN755_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7382703.1"
                     /translation="MFQQENQAQRQKEFQQAYANAYASGDRGALRQLANQYPDQIESV
                     RKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNSVAQMYQ
                     QNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQ
                     NLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAV
                     NNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALV
                     NSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAE
                     WMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQV
                     GSYTSKSGIQFTVE"
BASE COUNT          777 a          851 c          745 g         1019 t
ORIGIN      
        1 tggctgagaa acagggacgt gagatccgtc ttcattctct acataaacct gaatctggtt
       61 ttcaggattt accgggtcag tgtcaatttt tccgatataa attttgccat tggctacggc
      121 tttaaaagaa cgagccatag tgaagagttg cgaaggcatc gatacgatca cattggctgt
      181 aatgtctgtc atttaatttg ctccagatac aatgaatcgc cgcagcgtgg ctacggtgaa
      241 ttttgggcat aaaaaaaacc agccgaagct ggatagttgc gttggttatc tgtcagtagt
      301 tatgtgctta tttaattatt tatctatcta ttgttctgct gtaatttggc ttgaaagtat
      361 agggcgtatc gcatttgcag cattatttaa cgctctttca taagctgccg ttcctgcttt
      421 ggtgtttgcc aaacgaagaa gcatattcct tgcggctttg gactcataca aacgcatcat
      481 tgcaccaaaa ccagcctcaa gccccattga tacaccaaga gtcgcagttg cgccaatcgt
      541 ccttatcctg ttggcttgcg attgccccgt ctgagttact acatttgcgg tgtctgacct
      601 tgctgtttgc tgtagaactt catgaagagc atcaagctct ttcatgtgct ttccagaaaa
      661 aatagtgttg taaatttcac cgcctgactg agatttcagc ttattaactt cagtgatgaa
      721 cttggctgga gagtcaccgg ccttttccgc tattttgctg acgtaagctg cacgcatagc
      781 atctttccct ttatcatcca gtgcgctcca gattcgtttc acgtcagatg gttttctgct
      841 taatacaacg gtatttataa gttcaggact ggcttcactg cttgccttgt tgagcttgtt
      901 agcaatgttt ttattaagca ccttattata aacgtttgca taatcggaat ttgctttaag
      961 gtattttgct gcgtctgatg caccgaggtt tttagcaact gcgttacgaa ggtcttttga
     1021 cattgcattc tctaccatat tggtagctgc ttttgcctgg ttggggaaga ccatagcatc
     1081 tccctgaaca ttagatctaa atgctgttct gtgctgacgc aagagatcaa acgtaacatc
     1141 caaatcagtt gcagggtttg ctaattcttc acgtaggtta cgcaaggatg taagcaggct
     1201 ttgattggca gacgtcccaa gccgttcctg tcttgcgatc gctgtattca gagcattcat
     1261 ggtatttgtg gtatcaactg cggcattacc cattttattg gtgacgtcat tgataacagc
     1321 gccagcggca tccttccgcc cccttaacgt ggtggtaaga gatttcacca catcatcagg
     1381 gttgtactca ccaaaacggt caaaataatt acttaccagc ttactacgcg ttgcatattg
     1441 ctccgctcgc tttgagcctg tcccgagcaa agccccctcg gcatcctgag ttaggccgcg
     1501 agtgaaagca tttttcggcg ggataacatc agatgtcatt ggtgtcacgc ccatcgattc
     1561 tgatgtggca attttcttcg ccacttctgg cgcaatatca ccttttatag ccgttattcc
     1621 acgccctatt ccctttgctg ctgcggaaag aacaccctga gcggcaaggt taactccggc
     1681 atttttagct gcattttgtg cgaaatcgcc tttctgattt gcggcctctg ccagtgatcc
     1741 aatagccatg cttcctgccg ttccaactcc tggaactaaa tacccaccaa ttgtttcacc
     1801 ggcttgcgcg taggggtctg tcggtctgtc tactggacga taaacatcat ccaaaacctt
     1861 gggtccacca agcccctgac tgattgcatt aatcagactt gcgccgccct gcaatacgtc
     1921 aaatggtatg tttaccagac cacgaccagc ctgttctgca atttgccctg cactttgacc
     1981 accagtgagc caatcgccag cttgttgcat caatgatggt tcttcccgtg ttggtgcatt
     2041 attggcctga ttaactgttt gttgctgaac agcctgacca gcaaaatact catcaatggc
     2101 ggtgccaata tcttcggtgc tcgtaccatc aggaaaggta aatgtcttac cgtttgcagt
     2161 tactttcatc attccaccgt aaattgaatg cctgattttg aggtatatga tccaacctga
     2221 ttccgtggtt ctcctgaagg tgtcgaatct tgtgctggcg ctgcgtcagt attcattgac
     2281 atataccgct taacggcact ccccaatgat tcaccttttt taacatccaa ccccaatatc
     2341 tgaccgccat tacgcgattg tccagggttg ccattcgcgc tcatccactc ggctttaaac
     2401 tcattaaact gcgcgtttcg tcgctcaagg tttgccattg catcaagcca tcttgcgacc
     2461 gtctcagggt tatccatgtc agttggcgca ccctgtcgaa cgatctcaac gtctttatcc
     2521 gttgctgggc cgggaggtag gaatttaaga acctgactgt taacaagggc attttggcga
     2581 atgcgcaaat cacgcaatgt tgtatcgctt ccggtaagtt ttgcgaacat gttctgtgcg
     2641 ttaccgaaca aacctgtcgt tggtttttct gctctgaact gttgagcaag cgcactcata
     2701 gaattggctg agtttgatga tgctgtagca ttgtttacag ccgtctcgat gcctttttcc
     2761 atgtttactg acagcttagg tgcttcgcta atcaactgct gagccttttc ctgcgcttgc
     2821 tgcatcttaa acccgaactc ttgctgatcc agagccaagc gttgtgctgc gatattgtgc
     2881 ccagtcattg ctgactgata ggaaaggttt tgccctctcg cctgaagtgc ttctccagcc
     2941 tgattgctgc ggattgtctc tgccagcctg cctcggtcaa tttcacgacc agccatcttg
     3001 tcctgaacat tgaagtaatc aatcggacca agagcagcca ttccaaggtg atcaacaaac
     3061 tcaccaaatc ctgaaggatt ctgctgatac atctgagcaa cgctgttagg gtcaacaccg
     3121 acgcgcgcca gctcattggc gttgttttgc agccatgatt gcattgcttc tggagacgag
     3181 gccgcaaggc gtgcgccagc cgctaaggtg ccgatagaat tacgctggtc ttcatcaatg
     3241 aatcccatgc ctttacgaac ggattcaatc tggtctggat attgattagc caactgacgc
     3301 aaagcaccgc gatcaccaga cgcataagca ttagcgtacg cctgctgaaa ttctttctgc
     3361 cgctgagcct gattttcctg ctgaaacacc cc
//
LOCUS       DADLTI010000271         3379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLTI010000271 DADLTI010000000
VERSION     DADLTI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.275"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..933)
                     /locus_tag="JN755_004131"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="JN755_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..1881)
                     /gene="rbsK"
                     /locus_tag="JN755_004132"
     CDS             complement(961..1881)
                     /gene="rbsK"
                     /locus_tag="JN755_004132"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7382704.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2187..2969
                     /locus_tag="JN755_004133"
     CDS             2187..2969
                     /locus_tag="JN755_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY7382705.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2971..3069)
                     /locus_tag="JN755_004134"
     CDS             complement(2971..3069)
                     /locus_tag="JN755_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY7382706.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT         1000 a          772 c          672 g          935 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgctcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tcaagattac gaatagtatt
     1681 atccgcaaaa atatcatcac ctactttagt cagcatcagg acttttgaat tcaatttagc
     1741 cgccgccacc gcttgattag cacctttccc accacatccg attttgaagg caggtgcttc
     1801 cagagtttct ccttctttag gcatctgatt agtgtaagta atgagatcca ccatattgga
     1861 accaataact gcaatgtcca tttcactacc tcttataaac tttcgcataa caatggtatt
     1921 taaataacat tagcatgtta cttttgcatc atttgtgact gagatcgcga ttagcacatc
     1981 aacccgatgt ttatttaata gacttccagt ctcatcactc aggccaacac tatctaatca
     2041 taagcaacct aacaagatta gtgcccaaaa ttcagcagcc tatacccttt tcatttcaaa
     2101 ggggcggtcg tatagtatgg taatgaaaac aatgtttact aacgccaaaa tgttattttt
     2161 ataacattct tacggagaga gagttgatgg aaacgaagca aaaagagcgt atccgacgtt
     2221 tgatggaact gcttaagaaa accgacagaa tccatttgaa agacgcagcg cgaatgctgg
     2281 aagtttctgt aatgactatt cgtcgcgatc tccatcagga agatgaacct ctgccactga
     2341 ccctactggg tggctatatt gtaatggtga ataaacccgc gctatccatg ccagtaatcc
     2401 atgacgttcc aaaaaatcat cgtgatgact tacctattgc aattctggct gccggaatgg
     2461 ttaatgaaaa tgatctgatc ttctttgata atggccagga gataccactc gttataagca
     2521 tgatcccgga tgcaatcacc ttcaccggca tctgttactc acatcgcgtc tttgttgcgt
     2581 tgaatgaaaa gcctaatgta acagcaatac tttgtggtgg tacgtatcgt gccagaagtg
     2641 atgcttttta cgatgccagt aactcttcgc cattagactc tctcaatccg cgaaaaatat
     2701 ttatttccgc cagcggtgtg cataatcact ttggcgtcag ctggtttaac cctgaagatc
     2761 ttgccactaa gcgtaaagcg atgaaccgtg gactacggaa aattttgctc gcccgccacg
     2821 cgttgttcga tgaagtggcc tctgccagcc tcgcaccgat ctctgcattt gacgttctga
     2881 ttagcgatcg tccgttaccg gcagattatg ttacgcactg ccagaatggt tctgtaaaga
     2941 tcattacacc tgattcagaa gacgaatgac ttactgaaaa aacaccacaa tcttgttaaa
     3001 catcgtcggg ttggactgat tacgttgcac tttcaccaca tattccagct tatctatttg
     3061 gcttatcacc tactccagac gctggtcatc cttgaccagt agccagatat ggcttttgtc
     3121 gcagtcctga atcggcaggc agagaatgtc ttcaacgtta agagcgcgac gggcaaaagc
     3181 ccacaaaggt gagtcattac acccggatgg taaacaggca cttccttgtt gtagaagccg
     3241 ttatattgtc cataatgtct gggtttttaa tgcatactgg gtaacaaccg gacgaatctt
     3301 cgaatatcac tggcgctgcc agccggtttg aattcatctc acaaccctgc atagagcgaa
     3361 tctcttgcct gcacgtcac
//
LOCUS       DADLTI010000272         3293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLTI010000272 DADLTI010000000
VERSION     DADLTI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.276"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(342..515)
                     /locus_tag="JN755_004135"
     CDS             complement(342..515)
                     /locus_tag="JN755_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7382707.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            583..1198
                     /gene="tnpA"
                     /locus_tag="JN755_004136"
                     /pseudo
     CDS             583..1198
                     /gene="tnpA"
                     /locus_tag="JN755_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1526..2608
                     /locus_tag="JN755_004137"
     CDS             1526..2608
                     /locus_tag="JN755_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7382708.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3097..>3293)
                     /locus_tag="JN755_004138"
     CDS             complement(3097..>3293)
                     /locus_tag="JN755_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7382709.1"
                     /translation="EGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFT
                     ERFHNRFKITPRELRKASRE"
BASE COUNT          952 a          729 c          664 g          948 t
ORIGIN      
        1 ggagttgata cttcactttc tgcctgaata ctgatgtacc acttaccgca ggactggctg
       61 acagtgacat ttttcacaac acccgtgacc tgacggctgt tccggtagcg catccagcca
      121 agttttggca gaaaaatacg gctgttttcc tgatcgagct taacgccctg cggatagcgg
      181 aatgcatcat tctgtcaccg ctttttgaat caggggaacg ccgaccgctt ctggaaaaag
      241 tttttgcagg cctgctcagg gtctttcagt gactgttgca atggctgtga cggagcatct
      301 ttaatccatt gcgtttcagt ggcatttttc cactcaacca atcaggaagc cattttcgcg
      361 taagggatgt ctttgttacc ggccttatga ttctcattct gacgtgccag agtacgactg
      421 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gcgcctgttg accaccgggt
      481 cttaaccgga atttgaatac ctgtagtcgt ttcacagggc aaattatact ttggtctgtg
      541 aaaaaaactg atatccgacg cggaaggcac tgtgttttcc tgatgcatgc tcaccgggtc
      601 tttgttacca agtaccgacg ccagattttt gaccatgacg cgacagaaaa actacgtact
      661 tacttttcaa atgtatgtgc tgattttgaa gctgaactgg ttgaaatgga tggcgaacca
      721 gagcacgtcc atctgttaat taactatccc cccaaactgg cgatatccag tctggtaaac
      781 aaacagcctc aaaggcgtat cgggtaggtt actgcgacgg gatcggccag atattgctgc
      841 caggtattac cgcaaagatg ttttgtggcg tcccggctat tttgccagta gctgcggagg
      901 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgaa
      961 aaaacgagcc ttatatcccc gccctgaagg acgggctttt acggtgcact ggataagcct
     1021 ggagattatc tacacctgca tggacatcat tgtactatgc cccgtacatg ccccatcaca
     1081 tcctcaacat caaagtatta gagccttgca aaccattatg cgtctcagtt tgtctcatct
     1141 atcttacgca aaaaaaatag aactaatatc aagcggcgct atattgcgta ttaaataaaa
     1201 ccacctcaat tacattaata aatctaaata aatatcatac aaataatttc catggttatt
     1261 tttaggctat ttaaattaaa aaaataaatt atgagtcaat cataaataac aacctttaat
     1321 ataatttgac taccaaattt atattcgttc acaaaatagt catgcattgt gtgcaataga
     1381 aacagttcag ataaaaatag ggatctactt cacaatttaa atgagaaact aaaacttaca
     1441 tcttgaaata atctcattga ttggatgaat atttgtagtg cagtgacatc atttttaaat
     1501 aatagttcaa ataaaagggc tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc
     1561 tgcatcagta ctgatggcga tgtctgctca ggcagcagag atttacaata aagacagtaa
     1621 caagctggat ctgtacggaa aagttaatgc taagcactac ttctcttcta acgatgcaga
     1681 tgatggcgac acaacttatg ttcgtctggg cttcaaaggc gaaactcaaa tcaacgatca
     1741 actgactggt ttcggtcagt gggaatatga attcaaaggc aaccgtgctg aatctcaagg
     1801 ttcttccaaa gataaaaccc gtcttgcctt cgctggcctg aaattcggtg actacggctc
     1861 catcgattat ggccgtaact acggtgtagc atacgatatc ggtgcatgga ccgacgttct
     1921 gccagaattc ggtggcgata cctggaccca aacagatgtg ttcatgactg gtcgcaccac
     1981 aggtgttgca acctatcgta acaacgactt ctttggtctg gttgatggtc tgaactttgc
     2041 tgctcagtat cagggtaaaa atgaccgcac tgacgtaact gaagctaacg gtgatggttt
     2101 cggtttctcc actacttatg agtatgaagg attcggtgta ggtgcaacct atgctaaatc
     2161 tgaccgcact aatgatcagg ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa
     2221 tgctgaagta tgggcagctg gtctgaaata tgatgcgaac aacatctatc tggctaccac
     2281 ctattctgaa acccagaaca tgactgtttt tggtaataac catattgcca acaaagcaca
     2341 aaacttcgaa gtagttgcac aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta
     2401 cctgcaatct aaaggaaaag acttgggtgc gtggggtgat caggacctga ttgaatatat
     2461 tgatgtaggt gcaacctatt acttcaacaa aaatatgtcc acttttgttg attacaaagt
     2521 caacctgatt gataagagcg atttcacgaa agcatctggc gttgctaccg atgatatcgt
     2581 tgctgtagga ttggtttacc agttctaatc tgattactca aagatatgtt gtgggaggct
     2641 ttgcctcccc aacatataag tggcgccctc aagccacttt ccttagaagc actatcttgc
     2701 ttctgactat ataaattacc ctttattttc tgggacgttt caacgcccca tttttaataa
     2761 tactcagtga acaattaacg tatcaattag atttaataac aacgatatcc gtacctaacc
     2821 tgatatctaa tgtcataagc atccctttaa ttatcccctc aacctcttgt aaatttccca
     2881 ctcttgagct ccacatagtc caaacgccgg atgcaaaaaa aatcacacgt gcatctctga
     2941 aattgtgatg gaggaagtga gtatcaatta tatgtgctta taaacgttcc cgattggcac
     3001 agaagtcacc ggattgttca ggctccaata ggttgagtat gtcgtattta ttttccaaaa
     3061 taaaaatcga tgctgttgct ccttaataaa atacttttac tctctggacg cttttcttag
     3121 ctctcttggt gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt
     3181 gtaaccattt tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa
     3241 cgcattattt aacctcacgt cgtccaatat actttgaaaa cttattccct cgc
//
LOCUS       DADLTI010000273         3222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLTI010000273 DADLTI010000000
VERSION     DADLTI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.277"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(41..550)
                     /locus_tag="JN755_004139"
     CDS             complement(41..550)
                     /locus_tag="JN755_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7382710.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            679..996
                     /locus_tag="JN755_004140"
     CDS             679..996
                     /locus_tag="JN755_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7382711.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(1051..1431)
                     /locus_tag="JN755_004141"
     CDS             complement(1051..1431)
                     /locus_tag="JN755_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382712.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(1434..>3222)
                     /locus_tag="JN755_004142"
     CDS             complement(1434..>3222)
                     /locus_tag="JN755_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7382713.1"
                     /translation="ALNAVTEVVFTGDRGGEIRHRLARDAAGRLTAKETADGRTEYVH
                     DAADQLLEIRRQRHETDAPEVIRFSYDRLGRMLSEETAQGILTHQYDELGNRTATTFP
                     DGRTQRHLYYGSGHLQQINLDREVISEFTRDHLHREVLRSQGRLSTRQLYDPGGRLKR
                     RETYSGMRGVVPETFTDRQYSYSGEDELLKTRHSRRGETDYFYDPTGSITACRSEDEG
                     YLASWQYDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEFGRAVEKEGRSG
                     TQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNRTKFLWDGMRLA
                     QESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGAPEEMTDSDGKI
                     VWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYDPDVGRFMVTDP
                     IGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVTVRSRSDAEQLF
                     MDRYLGHNYKNMTGESGPSTKNLMEYLTGNKTKAGSYHWDDIKDPSVTKPPYRVSGHG
                     PGNPDGDLPHLQVHQHGGSVRHIFFSRET"
BASE COUNT          744 a          863 c          763 g          852 t
ORIGIN      
        1 tgcagacgta aatcctggcg aaaccaggcc agatgggtag tcataaaact cctgaatggc
       61 gcaagttaat cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag
      121 gataaagcgc catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac
      181 ggcgataact gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgcgggc
      241 gaaaataacc ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag
      301 aaagcaaaac aattacgcgt tggcggtact ggttgtaaaa atcatccaga ttttgccact
      361 gatgaatatc agccacaaac gggccaagtt tgcggtactc gggctgagaa tgggccagaa
      421 agacgagttt atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt
      481 tcagtcgatg taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt
      541 gtagattcat tactgctcct gaacggtata caggagtaag tgtagacgtt ggtaaatgat
      601 gtttgtgaag ttaatccgct agcagggcaa gattacgaaa gcgcgctccc cgcaagcaac
      661 taatcaaggt agttaatcat ggctgaacac gatattacgc cagaagtcac catttccaaa
      721 acacagcgcc gctataaagt cggctatgtc agagcccgcc accaggaccg caaaaccggt
      781 atagcccggt attatagcct gcaccttaag ggcgactggc tgaaagaggc cggatttgat
      841 accggacggg gtgtcacggt gaagatttcg gaggggggcc tgaccatcat cgctgacagt
      901 gacgaggtgc aggaactgcg ggaggaactt tatcaggtta aacaggtagt taaggggatg
      961 cgggatggga tgtttagtgt gctgaacgaa agttaaatat taaaaaagct gggaagattt
     1021 aaaaccttcc tagcttttta ttctgtctta ttataatgta tactcatcat cttccgatga
     1081 atttataaat tgctccaagt ttacatgaag agtacttaac ctagttttta actcattaag
     1141 tgaagcgata atttcatatt ttccaacata atcatatgat ggatcgtatt cattatctat
     1201 gtcagaagct aaaaatgatg atattcgtag tttaaattta gcttcatctt tattacattc
     1261 attggcataa ggtgaatgcc ctatcatagc atattcctct aatctaaaag gataagatgg
     1321 ttttaattca tcgatcaatg aaatttcaat ccatgttcta tatccaggca ttaatggact
     1381 aatttctttg agcaaaacct ttccacttaa taatgtattg agattgttca ttattatgtc
     1441 tccctcgaga aaaatatatg tctaacactt ccaccatgtt gatgaacttg taaatgtggt
     1501 aaatcaccat caggatttcc tggtccatga cctgacactc tatatggagg ttttgttaca
     1561 gaaggatctt ttatatcatc ccaatgatat gaacctgctt ttgttttatt tccagtaaga
     1621 tattccatta agttcttagt gctggggcct gactcacctg tcatattttt ataattatga
     1681 cctaaatatc tatccataaa caattgttca gcgtcacttc tagacctaac agtaactttg
     1741 gtttcactac tatctaataa acgattcgct ttttggttac taacagcaca tggagttaat
     1801 ccaagtggat cagcatattt tagaggatta ggtgcataac tgtagagatt taaaccgcct
     1861 ctcaacccta tcgggtcagt taccatgaac cgcccgacat ccggatcata gtacctgtgc
     1921 agattgtagt gcagccccgt ttccctgtcg aggtactgtc cctggtagcg caggttctgc
     1981 tccaccccgc cgtggtcctt ctcctgtatc gtgttacccc agacctgata ccccgtctcc
     2041 cacacgattt tcccgtcgct gtccgtcatc tcttccggcg cgccgttcac atccgtgtga
     2101 aaatacagta tccggttcgg gccctctttg cccgccttat ccacccgcgc cagcggttca
     2161 taactcccct ggtccctgta gatgtacagg ctgcctgttc cctccggcct gctctcctgc
     2221 gccagccgca ttccgtccca taaaaatttt gtccggttgt acggcttacc gtcagggctg
     2281 atgtgctgtt tttccgtccg cctccccagt gcatcgtaca cataccggta tgtcccccgc
     2341 gctgtcgtca cttccaccat ccggtgttcc gcatcataac ggtaactctg cgttccgctc
     2401 cggccctcct tttccaccgc acggccaaat tcgtcataac ggtagtgcac accacggtaa
     2461 gacatcagcc ggttgaacgg caccacgctg ttttctgccg tggccctttc ccccgcgcgc
     2521 ctccccagca ggtttcccgc cgcgtcgtac tgccagctcg ccagataccc ctcatcctcc
     2581 gaacgacacg ccgtgatgct ccccgtgggg tcgtagaagt aatccgtttc cccccgccgg
     2641 ctgtgccgcg tcttcagcag ttcatcctcg ccactgtagc tgtactgccg gtccgtgaac
     2701 gtctccggta ccaccccgcg catcccgctg tatgtctccc tgcgcttcag ccgcccgccg
     2761 gggtcgtaaa gctgccgcgt gctcagtcgc ccctggcttc tgagcacttc acggtgcagg
     2821 tggtcgcgcg tgaactcgct gatgacctcc cggtccagat ttatctgctg gagatggccg
     2881 ctgccgtagt acagatggcg ctgcgttctg ccgtccggaa aggtggtggc agtccggttg
     2941 cccagttcat cgtactggtg ggtcaggatg ccctgcgccg tctcctcgct cagcatccgc
     3001 cccagtctgt cgtagctgaa gcggataact tccggcgcgt cagtttcgtg ccgctggcga
     3061 cggatttcca gtagctggtc tgccgcatca tggacgtatt ccgtacggcc atcggctgtc
     3121 tcttttgccg tgagccggcc cgccgcatca cgcgccagcc ggtggcggat ttcaccgccc
     3181 cggtccccgg tgaacaccac ttccgtgacc gcgttcagcg cg
//
LOCUS       DADLTI010000274         3204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLTI010000274 DADLTI010000000
VERSION     DADLTI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.278"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..153)
                     /gene="mokC"
                     /locus_tag="JN755_004143"
     CDS             complement(<1..153)
                     /gene="mokC"
                     /locus_tag="JN755_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7382714.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICIPAVVAALVT
                     RKDLCEV"
     gene            682..1848
                     /gene="nhaA"
                     /locus_tag="JN755_004144"
     CDS             682..1848
                     /gene="nhaA"
                     /locus_tag="JN755_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7382715.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1914..2813
                     /gene="nhaR"
                     /locus_tag="JN755_004145"
     CDS             1914..2813
                     /gene="nhaR"
                     /locus_tag="JN755_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7382716.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYAALFSPAVR"
     gene            complement(2851..>3204)
                     /locus_tag="JN755_004146"
     CDS             complement(2851..>3204)
                     /locus_tag="JN755_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382717.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          752 a          723 c          781 g          948 t
ORIGIN      
        1 aacctcacag aggtcttttc tcgtcaccag cgccgccact acggcgggga tacagatgac
       61 gatcagggcg acaatcatcg ccttatgctg cttcattgct ctcttctcct tgaccttacg
      121 gtcagtaaga ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa
      181 tatcactcgg ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa
      241 gcacccgccg ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg
      301 aagacgcctc gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt
      361 atctcttcat cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa
      421 gcatcgccga ctgacaacaa attaattatt acttttccta attaatccct caggaatcct
      481 caccttaagc tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa
      541 acgcatctca ccgctgatgg cgcaaattct tcaatagctc gtaaaaaacg aattattcct
      601 acactataat ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt
      661 cacctgaaag agaaataaaa agtgaaacat ctgcatcaat tctttagcag tgatgcctcg
      721 ggaggcatta ttcttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca
      781 accagtggat ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc
      841 gaaatcaaca aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg
      901 ttagtcggtc tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag
      961 gccgcatttc cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg
     1021 ccttttaact atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac
     1081 attgcttttg cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agcgctgaag
     1141 atctttttga tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg
     1201 ttctacacta atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta
     1261 ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg
     1321 gtgttgtgga ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc
     1381 ggcttcttta ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat
     1441 gtgttgcacc cgtgggtggc gtatctgatt ttgccgctgt ttgcattttc taatgctggc
     1501 gtttcactgc aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc
     1561 gctggcttgc tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt
     1621 ttgaaactgg cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc
     1681 ctgtgcggta tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta
     1741 gatccagaac tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg
     1801 gtaattggat acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta
     1861 ccggggagcc ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc
     1921 atatcaatta caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg
     1981 gcgcagcgga ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg
     2041 aagagcgcct gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc
     2101 tgggagaact ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg
     2161 atattgtgaa ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac
     2221 tttccaaacg cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc
     2281 atcttcgctg cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac
     2341 tggatatgat catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg
     2401 tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt
     2461 tcccggcttg tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc
     2521 gcaaattgct taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg
     2581 atgatgccgc tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa
     2641 cgctttatgc atatgatttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga
     2701 atgtgatgga agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac
     2761 agcgaatctg caatacggat tatgctgcgc tttttagtcc agcggtgcgt taatcggcag
     2821 ctcccccaaa gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg
     2881 gctcattatt tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg
     2941 aatctgatta cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg
     3001 gatttcattc gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact
     3061 gttttctaac tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa
     3121 gtaatattca acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc
     3181 aaatcttata tgatgaaact gata
//
LOCUS       DADLTI010000275         3171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLTI010000275 DADLTI010000000
VERSION     DADLTI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.279"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            324..1976
                     /locus_tag="JN755_004147"
     CDS             324..1976
                     /locus_tag="JN755_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7382718.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(1987..2157)
                     /locus_tag="JN755_004148"
                     /pseudo
     CDS             complement(1987..2157)
                     /locus_tag="JN755_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2290..2409)
                     /locus_tag="JN755_004149"
                     /pseudo
     CDS             complement(2290..2409)
                     /locus_tag="JN755_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2483..2809)
                     /locus_tag="JN755_004150"
     CDS             complement(2483..2809)
                     /locus_tag="JN755_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7382719.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
BASE COUNT          910 a          689 c          716 g          856 t
ORIGIN      
        1 tctccaggaa acttggggcg gttcacgtca gtatcagacc atattaattg ctgaaactga
       61 taaacatcaa accttataga agggtgaatg ctgaaagggg gctatggtga tcgacgtgat
      121 gacaacgaca ggtaagaccg tgacctgata aacctgaatt gtgtcctaga cataaagtcc
      181 gtgaagggaa taaggctcag gtcagcgaat cttatagtgg cccgcagctc tgactgcaat
      241 aaggccggat acagagcagc aactactcgt cgatgtcaga acaacggaag ccttgcaaac
      301 ggggatgcgt tcatacaggt acaatgatac cgttaaaaaa aaacattacg ctaataatgt
      361 tcaccctgtc attactgaca ggaaatcctg ctatagcgta tgaaacagat aaagtatata
      421 aaataaccgt tctgcataca aatgaccacc atggacactt ttggcgtaac aatcatggtg
      481 agtacgggtt atcctcccaa aaaactctgg tggacaatat ccggcagaaa gtaataaata
      541 atggcggaag tgttctatta ttatctggtg gtgatattaa caccggtgta cctgaatcag
      601 accttcagaa ggctgaaccc gatattcgtg gaatgaatct aattggttat gatgcaatgg
      661 ccgtcggtaa tcatgaattt gataatcctc taaatatatt acgtcagcag gaaaaatggg
      721 ccacatttcc tttcctttct gctaacatat atcaaaaaag tacagggagg cgtttattca
      781 gtccatggaa aattttcatc cgtcagaatt tgaaaattgc agtcattggc ttgacgacag
      841 atgatactgc gaaaacagga aattctgaat attttactga tattgagttc cgtcaacctg
      901 ctgctgaggc gaggagtgtc attgatgaac ttaatcagca ggaaaagcca gatattatta
      961 ttgcggcaac acatatgggt cactatgaca atggcgagtc aggttcgaat gctcctggtg
     1021 atgttgaaat ggctcgcagt ttgccgactg gctcattagc aatgattgtg ggaggacatt
     1081 cacaagctcc tgtctgcatg gccagcgata ataaaaaaca gtggaattat attccgggga
     1141 caacctgtgt accagataaa caaaatggca tctggattgt acaagcgcat gagtggggaa
     1201 aatatgtcgg tcaggctgat tttgaattct gtaacggtac aatgaaactg gttaattatc
     1261 agttacaccc agttaatctc aaaatgagaa ttaccagaga agatggaaaa acggaatttt
     1321 ctttttacac ccccgaaatt actgaagatc cacagatgtt atccttactg acaccattcc
     1381 agaataaagg caaagcacaa ctggatgtga aggttggggt tgtgaacgga cgtctggaag
     1441 gggacagaag taaagttcgt tttgttcaga ccagtatggg ccatctgatt ctgagcgctc
     1501 tgacagaacg tattgacgca gattttgctg ttgtcagtgg tggggaaatt cgagattcca
     1561 tagagagtgg taacatcaca tacaaagata tactgaaagt tcagccattt ggtaatacgg
     1621 tagtaagtat tgatttgacg gggaaagaag tagctgacta cctagctaca gtggcacaga
     1681 tgaaaccgga ttccggagcc tacccacaat ttttgaatac cagctttgta gtcaaaaaag
     1741 gaaaaatcga aatgctaaaa ataaaaggta aatcggttga ccttaacaaa aaatatcgta
     1801 tgacaacatt tagctttaat gctactggtg gggatggcta tccacgtatt gataatagac
     1861 ccggatacat aaataccgga tttattgatg ctgaagtact tatcgaatat atccggaaac
     1921 attcaccgct ggatgctgct tcatatgaac caaaaggtga agtcagttgg cagtgataac
     1981 gtaattgacg atcatcacgt gttttttgct gacagtcgct gtgactttgt gaaagtagtc
     2041 tctgcggatg tttgcgatat gggaatgcag ttgctgtatt ttgtgtttct gcttctgcca
     2101 gttgttgctg aatttaacct tgcggcttaa ctgatgttgg agcgctgcca gcttcttcgt
     2161 caatttcgct gattaaagtt tattcattat ggcatgatct gcgtaagcgg ctcgtcagaa
     2221 ccatattgat atttactaag agctcagatc aactttccgg ggcaacagat cgcgtacccg
     2281 gtttgccggt cagtcctgga tatgctcaat gacgtaacgc agccattttt ctggctccac
     2341 attgttcaga cggcatgtgc cgatcagcga gtacaacacc gccgcatgtt cgccaccgct
     2401 atcggaaccc cgccggagag agtttcactc tccggctgct cacggaaccg tacgtgacac
     2461 tctcgcgtca tacggctctt gatcactcat tcgatgcacg tagtaaatac cagtgggcaa
     2521 atagcttcgg atactgtttc cttaccagcg ccagctttcg ctgagccttc cggttcgaaa
     2581 gtctgtattt agactgcatc cacttaagca aacgtgactg cattgcactc cacagtcgat
     2641 agctgaagtt tctggaccag aactttccat agtactcaat ccagcctctc actatcgcat
     2701 tgtagcgcct cgcaaaatcc agcaaactct cagctgttga gcgatgtata cgccacttct
     2761 tgattgtttc tgttattttg cacattgctg catttgatgc acccggcata cattttctgt
     2821 acaattcgcc tttgaaattc ttcagagtac gcactttgaa gtcatatccg agaaaggaga
     2881 aactcgtagc gacgtttctc cttttaaacg tgtcaatgta ggcgatattc gtcttccctg
     2941 cgttcaggac caacccgact tccgagaagc gcaccgataa cctgttcttc agccttgttg
     3001 catcactcat tcgtgaacaa tgtactacaa gtcgagtcac ccgagggaac ctccccctct
     3061 ggtgctcacg gaaccggacg tgaacctctc agctcatccg gctcttctca ttgaggcctg
     3121 tccccatagt aacggccagt gtgcaaataa cctcgtttca ctccgcgcaa c
//
LOCUS       DADLTI010000276         3146 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLTI010000276 DADLTI010000000
VERSION     DADLTI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.280"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            21..215
                     /locus_tag="JN755_004151"
     CDS             21..215
                     /locus_tag="JN755_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7382720.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            402..836
                     /locus_tag="JN755_004152"
                     /pseudo
     CDS             402..836
                     /locus_tag="JN755_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            861..995
                     /locus_tag="JN755_004153"
     CDS             861..995
                     /locus_tag="JN755_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7382721.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1240..2349)
                     /gene="fepE"
                     /locus_tag="JN755_004154"
     CDS             complement(1240..2349)
                     /gene="fepE"
                     /locus_tag="JN755_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7382722.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          935 a          765 c          652 g          794 t
ORIGIN      
        1 ctgacggaga acaacggaag gtgaacaaac aacaacaaac tgcgctgaat atggcgcgat
       61 gtatcaaaag ccagagcctg acactgcttg aaaaactgga tgctctggat gccgacgaac
      121 aggcggccat gtgcaaacga ctgcacgaac tcgcagaaga actccagaac agcatccaga
      181 ttcgctttga ggccgaaagt gaaataggaa cataacgaag ctgacggagg cggtcacggc
      241 tttcccgtgg gcgttttcgt cagcagacaa gcccgtcagg gcgcgtcagg gggttttagc
      301 aggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtata tactggctta
      361 atcatgcggc ggcagagcaa atggtgagag aacagcgaca tatgaggtgt gaaatgctgc
      421 acagatacgt aaggagaaaa tgcagcttcc ggcgctcttc cgcttcctcg ctcactgact
      481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtgtctgc tcacgcaaag acggatatac
      541 ggttatccac agaatcaggg gataacacca gaaaaaacat gtgagcaaaa aagaggcccc
      601 gaaaaaaggc cgcgccggtg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      661 aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
      721 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
      781 tccgctgtta tggccgcgtt cgtctcattc cacgcctgac actcagttcc gggtaggcag
      841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttaacgcca ttggcagcgg
      961 ccactggtaa acaggatgtg cgagagatgt attgagagtt cttgaagtgg cagccttact
     1021 gaggctacac tagaaggaca gatttggtga catgtctcgc acaagactgt taccacggtt
     1081 cagaagttcc ccaacttact gaaccttcga aaaaccacct ccccaggtgg ttttttcgtt
     1141 ttctagagta gaagactacg gctgtaaggg aatgatctca agaagatcct ttgaactttt
     1201 tataaaagtt gcttataaaa tgatgcatca gcacaacttt tactcaatca cactcatctt
     1261 tctggcctcc atggcatgac gtaaaagtat atagccacat gcaagcatcc ctccaatgac
     1321 agaagaaaga attacaatta tggcattact tggcccatcc tttctgaccg gtaacgttgg
     1381 gctcaattga tactgaaatg gcataaaatc aacatccccc actttcgcca caaccaattg
     1441 attcacaaca tattgacgat ttcgcaactc cccactcaat tccgaaacat ccttgattga
     1501 ttttttgatg ttcaatttag ttgctatacc atcagaaccg agagccacag gaaaatctgg
     1561 gtcatccttc ataatctgac cattgctgta tacaggtttt tttattccag ccgcattagc
     1621 aacctccagt gaataattga ggcgtcgaat atctgcattc agctggtttc taatttttat
     1681 ctcatccagt gcaagaattt ctttttcaac attagtttta acttctagtt tatttcttat
     1741 atcttccatc aaatccctta caacaaggga agaaacataa ttaatatacc cagacaacac
     1801 atcgtgagct tctttacttg ttggagcagt aaaactcaga gtccatgata catacaatga
     1861 tgacttgtcg tttttcttac tggcattatc atcaatcgac ttcatatttt tactcaactc
     1921 aacaattgca cgatgcagtt cgagagcgct aatgttattc tttttaataa tctccataac
     1981 ataaggagat gatctcatat actcattcaa cgaattaacg gactgaaatt tcttaataaa
     2041 cagagcaaat gcgctatcac gattgatatc aaaatccact cccagaacat gaagcttgga
     2101 aagctcctta tttaattcac tccattgaac agactcagca ggagtaacca cagcagaact
     2161 agtccatttt tgtggcaaaa caaaagaaac aagaaagcca atacaagcaa aaaatagaat
     2221 caccccaaca atttttattt ttgctgccca taaaacgcta atcaagccaa aaaggtcaat
     2281 ttcttcatta ccattcggca acatgttgta attagaaaaa tgttcgtcag agacctttga
     2341 gattttcatt gaaaaaaaca caatatatta acatatggtt tttttattct atcatatgtt
     2401 aggaaaaaac ctaaaaacca ttcagcagac atttaacata atatcttatt gcgcgcaccg
     2461 ctaaaacgcg ctgaacgcga ttctgccgca aacaccgcaa aaagagatgt tttccccggg
     2521 gtaaaccgga aaaatgcgtc aggatcgctt tcagctcact gcatagctat gcatgaaagt
     2581 gaatgacgat cggtttgggc gcatacagtg cccatgagac acattttcgg acattttcag
     2641 ttcgtgaatc cctgactcat tgctacatat ctgaacagac acccctagca gcgcccccag
     2701 gggcgtcctg taaaaaaaca caccgcgccg ctagcagccc cccctaatat aagatgatgt
     2761 ttttttatga aaatagtcag cccccccccc taaaaagcgg tgttggcgca gctacaacac
     2821 cgcgccgaca ccgctttttt aaatatcaca aagagagcaa gaagaactaa tttgtcataa
     2881 ctctctattt ataaagaaaa atcagcaaaa acttgttttt gcgtggggtg tgggcatttc
     2941 aggcatgaga acagactcgc gtttttccgg agtatcagcg gaattattga ttaaggaaca
     3001 ctcgacgcag agcgaaattt tatgcaacag cggctgcact gcacagggat tatttatcag
     3061 ttgatatgct gtgccatgat acgtcctaaa gaaacaggac aggatgtgca attgtaatac
     3121 cgtcgcccgc aacgtcttac aattac
//
LOCUS       DADLTI010000277         3142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLTI010000277 DADLTI010000000
VERSION     DADLTI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.281"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JN755_004155"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JN755_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7382723.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JN755_004156"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JN755_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JN755_004157"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JN755_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7382724.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JN755_004158"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JN755_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7382725.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JN755_004159"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JN755_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          762 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADLTI010000278         3137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLTI010000278 DADLTI010000000
VERSION     DADLTI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.282"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..71)
                     /locus_tag="JN755_004160"
     CDS             complement(<1..71)
                     /locus_tag="JN755_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7382726.1"
                     /translation="MASISISCPSCSATEGVVRNGKST"
     gene            263..1240
                     /locus_tag="JN755_004161"
     CDS             263..1240
                     /locus_tag="JN755_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7382727.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1586..>3137)
                     /locus_tag="JN755_004162"
     CDS             complement(1586..>3137)
                     /locus_tag="JN755_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HAY7382728.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT          596 a          950 c          988 g          603 t
ORIGIN      
        1 gtacttttac cgttacgcac cacgccttca gtagctgagc aggagggaca actgatggag
       61 atggaagcca cgggagcacc tcaaaaccac catcatacac taaatcagta agttggcagc
      121 atcaccgtaa tcgtacagct aagaacaagt gcttcaagtc tacttattaa aatgtacctt
      181 tgaaaaagta gactttctgc ggtacgctct gacagtaagt ttgcccccga ttttttacgc
      241 agcgcgccgg acggagcccg acatgatcct gattcccgat gacgaaccgg agctgagtct
      301 gcccgcagcc agtgaggaat tcctgccggc gctgtcaggt gagaatgccc cggtcagccc
      361 ggcccgggcc tacctgcttt cactcaattc ccaccgcagc cggcagacca tggcctcgtt
      421 cctgaacatc gtcgccgtta tgctcggagc tgcctccctg gagtcctgca gctggggcag
      481 cctgcggcgc catcacgtga tggccgtgac cgaactgctg cgcgacaccg gccgggccac
      541 cgccaccgtc aacacctatc tttcggcact caagggcgtg gcgaaggaag cctggatgct
      601 ccggctcatg gatgtggaga gcttccagca tatccgggcg gtccgtaacc tgcgcggcag
      661 tcggctgccc agcggtcggg cgctgcccca gggggagatc cgcgccctgt ttgccgtctg
      721 tgaagccgat cgcagctgcc tcggggcgcg ggatgcagcg atgctggcgg tcattctcgg
      781 ctgcggcctg cgacgatccg aagtagtgag cctggatttg cgtgacgttg tcactcagga
      841 ccgtgcgctt agagtgctgg gtaagggaaa caaggagcga ctggcatatg tcccggccgg
      901 tgcctggcag cggctgcaga tctggatcga tgagatccgg ggcgagaccc cgggcccgct
      961 gttcacacgc atccgtcgtt tcggggatgt gactctaaac cggctaaccg accaggcggt
     1021 gtaccatatc ctgcaggtgc gccagggtca ggccggcatc acgaaatgtt ctccacacga
     1081 tctgaggcga accttcgcca ccgcgatgct ggataacggg gaggatttaa ttaccgtgaa
     1141 ggatgcgatg ggccacgcga gcgtcaccac cacccagcag tatgaccgtc gcggggagca
     1201 acgcctgcag gacgcgcggg atagactcaa ccttatttag ggctaagtaa acacaaacaa
     1261 gggggctgta ccccctctaa ttttacatta acacgatact cgacagactt tcccagtcgt
     1321 agtcagcaac catcgcccga tggaggctgg gataaatgct gacagcccga cgtgaagcaa
     1381 aaggctctcg ccgctggcat ggcttaatgt cgcggtcccc tcaatcacta acacaatttc
     1441 agcgctggat gtcattaccg tttgattgtt caccgcagtg aaaatactga aatggaaatc
     1501 ctcaacagga ataggatagc gcgcttctgc ggcgtgctgt tccggtaaca taggggagcc
     1561 cgcagaattc ggaaaaaatc gtacgctaag gttttccggg catccgtaag ggccgaaact
     1621 tcccgtcttc cagtctgcgg ctctgccgcc agacgtaatc gccggttagg ttgatgtgct
     1681 cccagcccag cggcgacagg aattgcagca gctcgccgtc caccggcttg ccggcctcga
     1741 ccaacccctg ggtggcgcgt tccaggtaca ccgtgttcca cagcacgata gccgccgtca
     1801 ccaggttgag gccgctggcc cggtagcgct gctgctcgaa gctccgatcc ctgatttccc
     1861 caaggcggtt gaagaacacc gccctggcca gcgagttgcg cgcctcacct ttgttcaggc
     1921 cggcatgcac gcggcggcgc agttcaacac tttgcagcca gtccaggatg aacagcgtgc
     1981 gctcgatccg gcccagctcg cgcagggcca cggccagtcc gttctggcgc gggtagctgc
     2041 cgagcttgcg cagcatcagc gaggcggtga cggtgccctg cttgatcgag ctggccaggc
     2101 gcaggatgtc gtcccagtgg gcacgcacgt gcttgatgtt cagggtgccg ccgatcagcg
     2161 ggcgcaacgt cgggtaggct tgcacgccct gcggcacgta cagcttggtt tcgccgaggt
     2221 cgcggatgcg cggcgcgaag cggaagccta gcaggtgcat cagggcaaag acgtgatcgg
     2281 tgaagccggc cgtgtcggtg tagtgctcct cgatccgcag gtcggactcg tggtacagca
     2341 ggccgtcgag cacataggtg gaatcgcgga cgccgacatt caccacgcgg gtgctgaacg
     2401 gcgcgtactg gtcggagata tgggtataga acagccgtcc cggctcgcta ccgtacttcg
     2461 ggttgacgtg cccggtgctc tcgccccggc cacccgcgcg gaagcgctgg ccatcggagg
     2521 atgaggtcgt gccgtcgccc cagtgggcgg caaaggcgtg gcgatactgg tggttgacca
     2581 gctcggccaa ggccgccgaa taggtttcgt cgcggatgtg ccaggcttgc agccaggaca
     2641 gcttggcgta ggtcaggccg gggctcgact cggccatctt ggtcagcccg aggttgatcg
     2701 catcaccgag gattgcggac agcagcaacg tcctgtcttt ggcctcggcc ccgtccttca
     2761 agtgggtgaa gtggcggctg aagcccgtcc agtcgtccac gtccatcagc agttcggtga
     2821 tcttgatgcg cggcagtaac tggctggttt ggtcgatcag cgcctgcgcc cgatccggca
     2881 ccgccgcatc cagcggggtg attttcagcc ctgactcggt gaggatggca tcgggcagct
     2941 cgttgtcctt ggccaggcgg gtgacggtgg ccaactgctc gtccagcagc tgcaaacgct
     3001 cttccaggta ctggtcgctg ttcgggttga tcgccagggg cagggcctgc tcgcgcttga
     3061 gtgcggcgaa cttctcggcc ggcagcaggt agtcgtcgaa gtcgcggaac tgccgcgagc
     3121 ccttgaccca gatgtcg
//
LOCUS       DADLTI010000279         3082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLTI010000279 DADLTI010000000
VERSION     DADLTI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.283"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(143..871)
                     /locus_tag="JN755_004163"
     CDS             complement(143..871)
                     /locus_tag="JN755_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY7382729.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(1834..2487)
                     /locus_tag="JN755_004164"
     CDS             complement(1834..2487)
                     /locus_tag="JN755_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAY7382730.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(2480..>3082)
                     /locus_tag="JN755_004165"
     CDS             complement(2480..>3082)
                     /locus_tag="JN755_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7382731.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT          746 a          786 c          755 g          795 t
ORIGIN      
        1 ggctattagc tcgtctcggg attggtgctg gcgggtctca tcttccgggt ctagaccaag
       61 ctctctgagg aggacttcag tccggtagcc acggtaacgg agagtcatgc gcccctcagg
      121 tgtgcgcggg tcaatcatca tcttagattc cctccacctt accggattgt gccagcttca
      181 ggaggtgctc ttgcttctgc tggtcggtgc cagtgaatgc aataaggatg cctcgcatct
      241 tgtcaaccat ctcaggctta ccgaagacct ccagcccctt agcgttgaac cggaagttat
      301 acccacctgc tgagaacttc tggtctgacg gcttaatgat acgaaagcag tcggaatact
      361 tcggatttac agaccactcg ttactgattg ggttcttacg aaagaggcca cgatgctgga
      421 gaagtgcggt cattagcttc tgagcgggca gaccataggt tttgcagtag tcgccccaag
      481 tcacgcctcg gcggttagcg gactgaatgg ctgctcgctt cttctggtct tccagttgaa
      541 ggactcgacg ctctaacgtc tgaatatggt cgtaacactt ggcgcggagg tgaacggaat
      601 agccagcgac cagaatgaaa gtatgcttac ggtcgagacg atacagcggc tgctgtttcc
      661 cctgcgcgtt tagatagaca tcttcagtca ccccgatatg actgctcagt tttgagcact
      721 gctcagtttt gagcgcgtta agctcctcag ccatattgcg aatatcacgc aggacatttc
      781 ggtgctcctt gcctgtcagc tcagcaattt caactgaggt catggagaca agctggtcga
      841 ggttaatgac gctgatttct tgttgggaca tttacgtgta ctccttttgt ttgcaccctg
      901 aagtcgcacc ctactgacct actcaagccc ccacgtaaag ggagaccagc aggcaacagg
      961 acgcaaacag aatgacttga ggtaattgtg atgtggtctg cgatgaccag tagtgataaa
     1021 ggtgcaagac agcatgtccg acgtccagat ggtgagcggc gatagaccca agtcaaaatg
     1081 aagacacgag aacgctaacg ggctgggagt ctgacagagg ctgtcttgct agccttggta
     1141 tggagaaaga cttaatgtcg gggggcttcc gcataacttg acgcatcaag accagaggcg
     1201 gaatgtatgg cccctatagt gatagctaag ggtccattcc ctatagtgag ggtataagat
     1261 ttgctacctg ataaggccat aatctgacct ccagttagac ccgaagtatg gagaccacat
     1321 catagcgacc atagtttact tcgggatgac aggaggctgg acttaaagtt cttcttattg
     1381 gaattgtgat aaacacaatc cataacaaga taaactcatg gtctcctcca gcctgctggt
     1441 caggactcaa ggttcaacct cagacaccac tggtctaacc tccttgagtt tccgcctgtc
     1501 cacaatggat ggcagacctc agcaatgatg tcttggttct tcacaatctc gcacaccgat
     1561 ggtctcatag acggaagttc cgctgaggtc tgatgttctc cgcctgtcaa aggagagaga
     1621 ttgctacgtt aaagggaatg gtctcaggcc aaggtgattt aacactaggc gtctgcggat
     1681 ggtttccaat cggtccgcta ccggaagcct gagacttaat gaatgtgtgt gggtcgtgaa
     1741 tgttattaag gtggggactt agggtctctc ccctatagtg atagttaagc ccccttccct
     1801 atagtgaggg tatattcaat ctgacctaaa gctttactcg ctagctgcct gctgctgttc
     1861 cctcgccttc ctgtcccggt cctcaatgcc cttgagcgcc ctttccggca tgctgttaaa
     1921 catggcttcg acttctttcg gtctggtaat ctgctcagtg acaataatgt tcagcagctt
     1981 aaaaagacca tgcgctaact gagggtcgtc atcaatatta atttcacccg gatggaccgc
     2041 ctgattgcca acgatacgac aaatatcagc agctcgctgg accaatggag acaatccttt
     2101 ggctacaagc gacttaatgt ccgtgtcgat atgcttcccc ggctcaccga gatggaccat
     2161 cagcttctga agacttaagc gcaggagtgc ggcagcgcct ttaggagaaa ggttaacaat
     2221 gcttctggcc tccatgtagt cggacttaca gtcgtctggc atgtctgggt taggcatctc
     2281 aacgtttgct gacgctggta taatcatggt ttcgtcatac caataagctt tctcattgca
     2341 tcgttcacat ttagaaaagg cgaaaggtgt ctcataaatc ccgttagcgc catattttaa
     2401 gcggacccag cgctgttgag aataagcgcc acaattcggg cagttaaaag cagtgccacc
     2461 aaactctgcc ggataatact cagccattta ctccagtctc cttataaaca cccgccatca
     2521 cattaaccaa tctatctacc tcgacctctt tcgcgtaacg gtcgtaagtt atagacccag
     2581 agctatgacc cataatcgcc tgagcatacg ccaattcgac cccatgttgt tttagctggg
     2641 tcgcaactgt atgtctaaac gagtgaaaag ctaagccgct acggtcagca aggactttag
     2701 gtagcaacgt tcggttaaac catttgctca cctgctcacc gtagccgttc ttcatcagct
     2761 tgaggccatc gaataccata gcattatcac cctcaatctt tctacggtct tctacgagtg
     2821 atataaagtc agccaataca aaaccataag caccatcgac cagcgggaca cagcggtcac
     2881 tatgggcgtt cttgatactc ttacccggca ggctgctatc gtcttcgttg atatggataa
     2941 acacggtccc ggcctcagtg gtcctgacat ctttgacctg aagttgggcc acctcgttaa
     3001 gtcttgctcc agtgattgca gccagagcgg tgacgtaata atggtatggc ttacctgcgt
     3061 tcttctgaga gtacgcttta gc
//
LOCUS       DADLTI010000280         3050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLTI010000280 DADLTI010000000
VERSION     DADLTI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.284"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..464)
                     /locus_tag="JN755_004166"
     CDS             complement(<1..464)
                     /locus_tag="JN755_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7382732.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVK"
     gene            complement(511..1230)
                     /gene="yehT"
                     /locus_tag="JN755_004167"
     CDS             complement(511..1230)
                     /gene="yehT"
                     /locus_tag="JN755_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7382733.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="JN755_004168"
     CDS             complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="JN755_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7382734.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          787 a          811 c          700 g          752 t
ORIGIN      
        1 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
       61 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      121 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      181 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      241 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      301 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      361 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      421 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
      481 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
      541 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
      601 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
      661 gcgcagtagt ggtgtacgac tttccagggt acgtaatgtc aattcggtaa agccctcttt
      721 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
      781 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
      841 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
      901 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
      961 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1021 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1081 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1141 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1201 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1261 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1321 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1381 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1441 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     1501 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     1561 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     1621 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     1681 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     1741 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     1801 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     1861 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa acggtttttg
     1921 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     1981 agagtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2041 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2101 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2161 gtaatcgcga ctgcaccaat atccagctcc tgatacagca cctgagccac tttcatgctg
     2221 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2281 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2341 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2401 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     2461 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     2521 cgacggatca ggatgctgtg tactaggccg ccgagcaatc cttcaacgat ggttgagatc
     2581 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     2641 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     2701 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     2761 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     2821 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     2881 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     2941 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3001 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DADLTI010000281         3037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLTI010000281 DADLTI010000000
VERSION     DADLTI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.285"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            52..1083
                     /gene="ynaI"
                     /locus_tag="JN755_004169"
     CDS             52..1083
                     /gene="ynaI"
                     /locus_tag="JN755_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY7382735.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1134..2747)
                     /locus_tag="JN755_004170"
     CDS             complement(1134..2747)
                     /locus_tag="JN755_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7382736.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          791 a          705 c          671 g          870 t
ORIGIN      
        1 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
       61 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
      121 attctgatga gcttttggtt tcgccgtgga aatcgtaaaa gaaaaggatt tttattccat
      181 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat cattaattat
      241 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
      301 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      361 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      421 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      481 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      541 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      601 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      661 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      721 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      781 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      841 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      901 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      961 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
     1021 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acagggtcgc
     1081 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
     1141 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
     1201 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1261 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1321 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1381 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1441 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1501 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1561 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1621 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1681 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1741 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1801 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1861 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1921 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1981 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     2041 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     2101 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     2161 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     2221 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2281 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2341 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2401 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2461 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2521 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2581 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2641 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2701 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2761 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2821 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2881 ttagtgtaaa taatagacgc caaaatgcgg caaagcacat agaacaccga acactacgtt
     2941 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     3001 gaagaataaa aaataaccaa aacgttgaaa ggaactg
//
LOCUS       DADLTI010000282         2989 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLTI010000282 DADLTI010000000
VERSION     DADLTI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.286"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(39..1718)
                     /gene="bcsG"
                     /locus_tag="JN755_004171"
     CDS             complement(39..1718)
                     /gene="bcsG"
                     /locus_tag="JN755_004171"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7382737.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1715..1906)
                     /gene="bcsF"
                     /locus_tag="JN755_004172"
     CDS             complement(1715..1906)
                     /gene="bcsF"
                     /locus_tag="JN755_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7382738.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1903..2989)
                     /gene="bcsE"
                     /locus_tag="JN755_004173"
                     /pseudo
     CDS             complement(1903..2989)
                     /gene="bcsE"
                     /locus_tag="JN755_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          750 a          772 c          784 g          683 t
ORIGIN      
        1 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
       61 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      121 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      181 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      241 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      301 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      361 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      421 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      481 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      541 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      601 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      661 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      721 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      781 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      841 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      901 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
      961 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1021 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1081 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1141 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1201 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1261 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1321 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1381 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1441 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1501 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1561 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1621 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1681 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1741 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1801 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1861 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1921 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     1981 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2041 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2101 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2161 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2221 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2281 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2341 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2401 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2461 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2521 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2581 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2641 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2701 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2761 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2821 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2881 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     2941 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DADLTI010000283         2962 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLTI010000283 DADLTI010000000
VERSION     DADLTI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.287"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JN755_004174"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JN755_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7382739.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JN755_004175"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JN755_004175"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7382740.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2962
                     /locus_tag="JN755_004176"
     CDS             2604..>2962
                     /locus_tag="JN755_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7382741.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          660 a          703 c          781 g          818 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattt tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg tgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gt
//
LOCUS       DADLTI010000284         2870 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLTI010000284 DADLTI010000000
VERSION     DADLTI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.288"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN755_004177"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN755_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7382742.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JN755_004178"
     tRNA            complement(621..695)
                     /locus_tag="JN755_004178"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="JN755_004179"
     CDS             complement(771..1703)
                     /locus_tag="JN755_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7382743.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="JN755_004180"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="JN755_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7382744.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          695 a          697 c          689 g          789 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatgggtg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattacc atcgctcggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggttgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggtgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga
//
LOCUS       DADLTI010000285         2831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLTI010000285 DADLTI010000000
VERSION     DADLTI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.289"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..420
                     /locus_tag="JN755_004181"
                     /pseudo
     CDS             1..420
                     /locus_tag="JN755_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1004..1720
                     /locus_tag="JN755_004182"
     CDS             1004..1720
                     /locus_tag="JN755_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382745.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2272..2676
                     /locus_tag="JN755_004183"
     CDS             2272..2676
                     /locus_tag="JN755_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382746.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          816 a          544 c          626 g          845 t
ORIGIN      
        1 catgatcggg gaagacccag atatgaacag gcacattcag agttacttta aagagcttac
       61 tgaacgtctg aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat
      121 cgagcagcct ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg
      181 actcgggaac tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc
      241 tctgtacatg gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg
      301 cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg
      361 ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga
      421 aaactggatt gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa
      481 cgggcgagag catgacattg tctgttatta taatgagact gctgattcta cttattttta
      541 atttcaagaa aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata
      601 ttatcattct gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acgtccctct
      661 catcgaaccg gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca
      721 cgttcgggcg gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc
      781 agtagattac cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt
      841 gaccaagttt tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat
      901 gataaatatg cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc
      961 aacttttatc tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt
     1021 gcttagaaat tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta
     1081 ttcttatggc tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg
     1141 tttggatgat gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac
     1201 cggttggggg attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga
     1261 cttgagtttt tttagactat tttttgctat taccctactg tttttccctg taatattcga
     1321 acttttaatt ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc
     1381 attggttatt tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc
     1441 agatatccct tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta
     1501 tttttggctt ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca
     1561 gatgctttgt tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt
     1621 tttgtctagt tcatttaaag atgacagtaa cagattctta atatttaata cagaacagtc
     1681 gggattttat gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg
     1741 aattaaatga atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca
     1801 aaccttaggg cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt
     1861 gctcggaaca gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg
     1921 ccaaacatca caaatcacca gccggactga gtgatgccga gcattgcacc aataccccgt
     1981 gatgaacgat gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg
     2041 cgattcaccg tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac
     2101 tgaacaactc tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag
     2161 cttccacttc cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag
     2221 gacatgaggt gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact
     2281 gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact
     2341 ttactggcta tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga
     2401 ccatatgggg tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa
     2461 tgtaacagtg actactatta ctctgataaa ccaccgggag tacagatact cggaacccgt
     2521 gcagggtgtc gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga
     2581 ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg
     2641 aaacgtcctg tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg
     2701 cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca
     2761 gcaaaaaaac acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg
     2821 aacgcatcgg t
//
LOCUS       DADLTI010000286         2812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLTI010000286 DADLTI010000000
VERSION     DADLTI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.290"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..405
                     /locus_tag="JN755_004184"
                     /pseudo
     CDS             1..405
                     /locus_tag="JN755_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
     gene            393..839
                     /gene="etp"
                     /locus_tag="JN755_004185"
                     /gene_synonym="yccY"
     CDS             393..839
                     /gene="etp"
                     /locus_tag="JN755_004185"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7382747.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            859..>2812
                     /gene="etk"
                     /locus_tag="JN755_004186"
     CDS             859..>2812
                     /gene="etk"
                     /locus_tag="JN755_004186"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY7382748.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPM"
BASE COUNT          744 a          665 c          749 g          654 t
ORIGIN      
        1 caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat
       61 ggaccgcagc ggtatgaccc tggcagaagc gctgggtaac gctgagggta tgaatcagga
      121 tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa
      181 aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga
      241 attccagctt gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt cactaccgcg
      301 ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc cggggtgcat
      361 gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt aactcaatcc
      421 tagtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg ctgcgtaaac
      481 gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctggtaaaa caccctgcag
      541 atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga catgctggac
      601 gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg gagtcggaac
      661 atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg ctgtttgggc
      721 aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac gcatttgaac
      781 atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc agccggtaat
      841 ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccactagg cagcactcag
      901 gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg taagtttatt
      961 atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt aagcacacca
     1021 atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc cattctcagc
     1081 ggcctgagcg atatgctccc taactcatcg cccgagtctg caccggagat ccaactgctg
     1141 caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga catagttgag
     1201 cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga aaaaccaggt
     1261 gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca gcaactgaca
     1321 ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac cgtcaatggt
     1381 atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga cattaaggcc
     1441 aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat taacacattg
     1501 caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga acttaccatg
     1561 actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa caactatttg
     1621 caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt cttacagcac
     1681 cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa cgtttatcgc
     1741 cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga gcagattgtg
     1801 aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc ccagctgtat
     1861 aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct ggagcaagaa
     1921 cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga agtgttgcgt
     1981 ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa ccgccagcag
     2041 gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga cccggcagtc
     2101 actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg ttttattctt
     2161 ggcctgttta tttcggtgag tgccgtgctg gcgcgtgcga tgttgcgtcg tggtgtagaa
     2221 gccccggaac aactggaaga gcacggcatc agcgtttatt ccactatccc aatgtccgag
     2281 tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca gcagcgccat
     2341 cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc tgtggaagcc
     2401 gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agactgagaa taacattctg
     2461 atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac tctggcagcg
     2521 gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg ccgtggttat
     2581 tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct ggcaggtaaa
     2641 gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt gattactcgc
     2701 ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg tcaattactg
     2761 gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat gc
//
LOCUS       DADLTI010000287         2746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLTI010000287 DADLTI010000000
VERSION     DADLTI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.291"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            100..165
                     /locus_tag="JN755_004187"
                     /pseudo
     CDS             100..165
                     /locus_tag="JN755_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            393..641
                     /locus_tag="JN755_004188"
     CDS             393..641
                     /locus_tag="JN755_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7382749.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(757..1026)
                     /locus_tag="JN755_004189"
                     /pseudo
     CDS             complement(757..1026)
                     /locus_tag="JN755_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1083..1751
                     /locus_tag="JN755_004190"
     CDS             1083..1751
                     /locus_tag="JN755_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7382750.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2067..>2746
                     /locus_tag="JN755_004191"
     CDS             2067..>2746
                     /locus_tag="JN755_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7382751.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          836 a          593 c          541 g          776 t
ORIGIN      
        1 tggcattacc ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt
       61 ctaaagttaa actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt
      121 gaagtggcgt tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt
      181 aaaaatcgac gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt
      241 taccatgtcg acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat
      301 tgatgcccca taaacaatat caggaattga cactggttat ccatacagat aaaaataaca
      361 cctgtacata actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag
      421 aaaaaatcac taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac
      481 gcatcagtaa acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc
      541 tttctgttac acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga
      601 aagatacctg ggagtctgct gacgagtggt ttgttcacta ataaacacgt caaatcggta
      661 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
      721 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
      781 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
      841 tcaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
      901 aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca
      961 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
     1021 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
     1081 atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
     1141 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
     1201 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
     1261 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
     1321 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
     1381 aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg
     1441 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
     1501 gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa
     1561 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
     1621 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     1681 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     1741 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta
     1801 cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat
     1861 gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat
     1921 ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac
     1981 aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta
     2041 tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg
     2101 gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg
     2161 aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc
     2221 ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca
     2281 gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat
     2341 caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat
     2401 ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt
     2461 tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt
     2521 ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg
     2581 aagtaatacc agagtttcca gaaagagatg atcatattat aagaatattc tggcttaatc
     2641 agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca
     2701 atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgtt
//
LOCUS       DADLTI010000288         2742 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLTI010000288 DADLTI010000000
VERSION     DADLTI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.292"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..437)
                     /locus_tag="JN755_004192"
     CDS             complement(<1..437)
                     /locus_tag="JN755_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7382752.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVE"
     gene            complement(539..1387)
                     /gene="yiaJ"
                     /locus_tag="JN755_004193"
     CDS             complement(539..1387)
                     /gene="yiaJ"
                     /locus_tag="JN755_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7382753.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            1588..2586
                     /gene="yiaK"
                     /locus_tag="JN755_004194"
     CDS             1588..2586
                     /gene="yiaK"
                     /locus_tag="JN755_004194"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7382754.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            2598..>2742
                     /locus_tag="JN755_004195"
     CDS             2598..>2742
                     /locus_tag="JN755_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY7382755.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT          673 a          733 c          675 g          661 t
ORIGIN      
        1 ttctcgacat ccatgcactg caacgccttt gtcgggcagg cttcaacaca ggccggcccc
       61 gtctcccgat gccagcacag atcgcactta atcgccctcg cctttttgcg cgaagaaacg
      121 acctccatcg caccaaacgg gcaagccagc atacagcttt tacagccaat gcaacgtgtt
      181 tgttcaacga aaatatgccc atgttcgcgg cttatcgcgt caacagggca gacattcgcg
      241 cacggtgcat cttcacactg atgacaggct actggcgtgg tccagctgtg gtctttaatg
      301 acacgaatac gggaaataaa ctcgtctggt gacaacgcag cgcaatcctg attctcctga
      361 tgcgacactg cgcaagccac ttcacaggta cggcaaccga tacatttcgt cgcatccgca
      421 ataataaacc ggttcatgat cattcctttg cactaaaaca gcagagtgcc agagagcgcg
      481 tccctactgc tttgatccaa cgaaggaaat gttaaagttt tggcacttgt ccaaagcgtt
      541 atgtgagcgc gcccaggtca tccctgacag taaatcccag ttcattagaa atagcctgcg
      601 cggtttcacg cagtggtttc aggagatttt tctctcccac ctgtttcaga cgtgatgtcg
      661 aaagcgaaat cgacacggcg tacggcaccc gcccatgaat atcaaacacc ggaacagcaa
      721 tacaggagac gccgagttcg ttttcttctc tgtccatcgc cgctccgctt tcacgaatgt
      781 gcgccagttc gtcgaacatc gcgggcagct cggtaatggt attgtgggtt aacggctgga
      841 tctcatgctg atggttttcc cagtatgact tcacgtagtc cgggtgacca aacgccatgt
      901 agatcttgcc cattgcggaa cagtagagcg gcatatgctg gccaatatag gcacgggttc
      961 gcagcatccc ggttgtgggt tccagcttat aaatcaaaat agcgtgatcg tcttcgcggc
     1021 tggagaagtt aatggtttca ccagtggcga tgttcagtgc ctcaagatgc ggagcggcga
     1081 tatgaatgat attcagcgaa gacagcgcct tctgcccgac ggcaataaat ttggtagtca
     1141 ggcggtaact ccctgcggcg ggcgcggtgg tcacatagcc gcaggactgt aatccctgca
     1201 ataagcgatg gacggtactc ttatttaaac cagccagctc cgaaagatgc gccaacggac
     1261 aaccgtttgg atagttgctc aaaatctcaa tcagcatcaa cccgcgaaac agactctggc
     1321 ttccggctgg acgctctttt tcctgcgcca tctcgttctc tttttttccc atcacttctt
     1381 tccccatttt gtcgcgtcct gatggtagcg caaagtgtgc cgtagttcac gatctcgaca
     1441 gataatttat aaccacttga tttttatgtc ttttgaaatt catcaatcag attgctttgt
     1501 taaaaagtga tcgatatatt tgaaatctag tttcgcatat tgaaatttta agacaaaaaa
     1561 gcgatcaaaa aaacaaggaa gcctaggatg aaagtgacat ttgagcagtt aaaagcagcc
     1621 tttaatcggg tcttaatttc acgcggcgtt gacagcgaaa cggctgacgc ctgtgcagag
     1681 atgttcgccc gcaccaccga atccggcgtt tattctcacg gcgttaatcg tttccctcgt
     1741 ttcattcaac aactggaaaa cggcgatatc attcctgatg cccaacccaa acgtataacc
     1801 agcctcggcg caattgaaca gtgggatgcc cagcgttcga tcggcaacct gacggcgaaa
     1861 aagatgatgg atcgcgccat tgaactggct gccgatcacg gtattggtct ggtggcactt
     1921 cgtaatgcca accactggat gcgcggcggc agctacggct ggcaggcggc ggaaaaaggc
     1981 tatattggca tttgctggac caactccatc gccgtaatgc cgccgtgggg cgcaaaagag
     2041 tgtcgcatcg gcactaaccc gctgatcgtc gccattcctt ccacgccgat caccatggtc
     2101 gatatgtcga tgtcgatgtt ctcttacggc atgttagaag ttaaccgcct ggcaggccgt
     2161 cagctcccgg tcgatggtgg ctttgatgat gagggcaatt tgaccaaaga acctggcgtt
     2221 atcgagaaga atcgccgcat tttgccgatg ggctactgga aaggttctgg catgtcgatt
     2281 gtgctggata tgatcgctac tctcctttcc gacggcgcat cggttgccga agtcacccag
     2341 gacaacagcg acgaatacgg catttcacaa atttttattg ccattgaagt ggacaagctt
     2401 atcgacggtc ccacccgcga tgccaagctg caacgtatca tggattacgt tactaccgct
     2461 gaacgcgctg acgaaaacca ggccatccgc ttacccggcc acgaatttac taccctgctg
     2521 gccgaaaacc gccgtaacgg catcactgtt gatgacagcg tgtgggccaa aatccaggcg
     2581 ttatgaggag ataagtcatg atttttggac atatcgcgca gcccaatccg tgccgtttgc
     2641 ccgcagccat tgaaaaggcg ctcgattttc tgcgcgctac caacttcaac gtcctggagc
     2701 cgggcgttgt cgaaatcgac ggcaagaata tttatgcgca aa
//
LOCUS       DADLTI010000289         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLTI010000289 DADLTI010000000
VERSION     DADLTI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.293"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN755_004196"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN755_004196"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7382756.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN755_004197"
     CDS             complement(1725..2096)
                     /locus_tag="JN755_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7382757.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN755_004198"
     CDS             complement(2119..2463)
                     /locus_tag="JN755_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7382758.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          667 a          649 c          673 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttaccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttggacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLTI010000290         2663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLTI010000290 DADLTI010000000
VERSION     DADLTI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.294"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..286)
                     /locus_tag="JN755_004199"
     CDS             complement(<1..286)
                     /locus_tag="JN755_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382759.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTR"
     gene            complement(948..1934)
                     /locus_tag="JN755_004200"
     CDS             complement(948..1934)
                     /locus_tag="JN755_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382760.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2016..2507)
                     /locus_tag="JN755_004201"
     CDS             complement(2016..2507)
                     /locus_tag="JN755_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382761.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT          703 a          615 c          668 g          677 t
ORIGIN      
        1 ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc gtctttggta tagtgactct
       61 ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg ataattagga tacttatctg
      121 gatttaacga taaataactt ttatctgtta ctgcccccct ggaattaaga atgttaaata
      181 cagattcgct tgtcacttct gcctctgtcg cctggcatgt gccgcacgaa aatataccag
      241 cgagcagaag agttaatgca gatttccctt tcataaaata atccatcaat cgtttgttaa
      301 aagcgtagat aaccacttat tcaacaactt gaaaataagg tattttacct gggttgttac
      361 aaaaggattg cattgcgtaa acgcttttta tttacaaaaa aatggggaag tgttacagcg
      421 attgcgaaaa gcctgaagtg attgaattaa gataatatag aaaatcagat acagcaaaaa
      481 tggcagattg atcaggtgaa gataaattgg agcgggcgaa gggagccgag gacaactagc
      541 taacttcttg tttcttaaag actgatgaat ccagtggtta cagtgatggt tacacatgga
      601 gaggctaaag gtcaagactt gaagtacgta acattcccac aaaaggaata gaacacagtc
      661 acggactacc cggccctttg gtctgactga gggtcgggct acggggtaaa tcgtcaggct
      721 tattgtatta gatggtctct cacatttttc agtcaaaaac tcatcgtact ttaggccaga
      781 ccatcagcat gactatcaga gccagcttta gcaggaagac cacgagatag actttgtgta
      841 ggtccattag gtcctcctct ttccgtaccc atgaactaga ttactagtac aaaataaagg
      901 ccagacatcc agttacggaa gtctgaccaa taagtcgtta ttgttagtta gcttgtctta
      961 gtaaagctca ggcgtttgac tacaggcagg acagcagcag cgctgccagt ccccatctta
     1021 acagtcgttg cagcctcagc gaccacgaag accttaccga gaatctcaag gtcgtgagac
     1081 tgggtgttgc catctcggga taagaccaca gtaatattct cgccatcgtg acggaagcaa
     1141 ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga cgccacggtc tgcaccgttg
     1201 tagtaggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1261 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1321 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1381 gccttagcgg tcaggctacc agtcagggcc ggaagctcta cggtcttcag cagcttagag
     1441 gactccagaa cgtcaaccat cttagagtta atggtacgga ccacgttatc gcagatgttc
     1501 agagcatcct gctggagcgt cccccgagtg aaaccagtag aggacttctg gtcccagagc
     1561 tttgtctgat gagacacgag gatagcctgt tcaatcggct caacggtgat ttcagtacca
     1621 gacggagcgg tcacgacttc aggtcgctta ccaccacgga caggaacgac cacagagcgg
     1681 tcagccagag tgtgagcaat gattggcgtc ggtaagctgt tacgggtcgg caggacttct
     1741 acagggaacg gaatgttttc ctgagcgtct ttaagtacac cgttcagcat agccttaacc
     1801 gtcttagcta actggagggc atagacctgt gggtcagcgt tagtaggcag accttcattg
     1861 acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa taccgaactg gaaagcgtct
     1921 ttaatcgtca taataatcaa tctcctaaat tagtttaaat actgtgagtc ctcaaaactg
     1981 aggagtccct agcatcgggt cagggactgt gtgttttaca ggctgtccat ccagttaccg
     2041 cctgactcaa ggtcagcgac gatagcggca cgggagcggc cagcggaatg ggtagcactg
     2101 cggaggtttt caccataggt ccggcgttca gctttgacct gttcgcggcg ctgtagctct
     2161 ttctgccatt cgtcttcggt catgaacatt tcgcgggtac gctctttctg agacgccagc
     2221 tctttagcga tacgctcagc ctcgacacgc ttattcatct ggcggcgttg gtaggcggtc
     2281 atcaggtcat tactgaagaa gttaccaatg gtcacgccag cttcagccag agcttccagt
     2341 aactccattg cctcgcggct ggtagacggc aggcctgaca ggtagcccgg aatgagggtc
     2401 ttgatagcga gcgacagcgg atagggctga ccgtccagac tcacagtatt ctcgtagaga
     2461 ttgatgtcgg tgactgacag tttccagtct tggttattga ataacatagt aattactcct
     2521 cttgagagtt gagactatcg gtcgaccaca ggcgtgttca gtcctgcggc gtctccccgc
     2581 atcggtcatc tcaaaaggtt taaaattagg tattgagtag acgcgctact aacggtgtgc
     2641 tgacgacagc tagcaagcta gcg
//
LOCUS       DADLTI010000291         2649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLTI010000291 DADLTI010000000
VERSION     DADLTI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.295"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..247
                     /locus_tag="JN755_004202"
     CDS             <1..247
                     /locus_tag="JN755_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382762.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JN755_004203"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JN755_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7382763.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JN755_004204"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JN755_004204"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7382764.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..>2649
                     /gene="mdfA"
                     /locus_tag="JN755_004205"
     CDS             1991..>2649
                     /gene="mdfA"
                     /locus_tag="JN755_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7382765.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GR"
BASE COUNT          674 a          647 c          687 g          641 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taagccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccg
//
LOCUS       DADLTI010000292         2591 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLTI010000292 DADLTI010000000
VERSION     DADLTI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.296"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(122..1135)
                     /locus_tag="JN755_004206"
     CDS             complement(122..1135)
                     /locus_tag="JN755_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7382766.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            1392..>2591
                     /gene="ascF"
                     /locus_tag="JN755_004207"
     CDS             1392..>2591
                     /gene="ascF"
                     /locus_tag="JN755_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7382767.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICG"
BASE COUNT          598 a          695 c          653 g          645 t
ORIGIN      
        1 cgccgataca ttcacaaatc atgccagttt ttaatttgct gttatttaag gaaattaatt
       61 tctgtaatgc aggaaaaaag atgtcatcga cactagtgac gatgacatgt gatgacaatg
      121 tttatcgcga aagggcaatg agtgagtcgc ggcggatcag ttttccgcta aaggttttag
      181 gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt tcctgaatca
      241 tctcagtcac cggaattttt acgctggaga gcgccggaac gatatagggg gcaattgcga
      301 tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg
      361 ctttgatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta aatttagccc
      421 cacgttcgag caacgtttct accccttcgg ccccgctggc gggcgtccat ttaccgttag
      481 cgataagttt ttcattgagc gcaataccat gctgcgacag cgcgtcttta tacccggcaa
      541 gacgttcaat gctggtgggg gaatccatcg agccggtaag gaaagcaatc tcctgatgcc
      601 cggcgtttat caactctgcc acggcgttaa agctggtctg tttatgatcg caccagacgc
      661 tatggctgct attattgcgc aggcggcgat taagcaccat tatcggctga ctgtgcgcgt
      721 caatgatgtc atcaatctca tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc
      781 agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat
      841 ctgccaatag caactgtcgc cctttctctt ccgccattcg cgcggcatga aacagtaatt
      901 cactaaaata aatgccgtga taaagcgtgt tggtcactac cagccccagc gtctgagtac
      961 tcttcgccga cagattgcgg gccagcaaat ttgggcggta actgctctct tctaccgcct
     1021 gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc acgcgggaaa
     1081 cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc atcattttca
     1141 tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc
     1201 ggcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca attaactctc
     1261 ttgcttattt agtgacaact attcatgatt ttgtgaaacc ggtttcttaa ttccgtttca
     1321 gcatcggtat ttttccgtca cgtcgactga taacaactac atctacccta ctgataacag
     1381 gataaaatcc gatggccaaa aattatgcgg cgctggcacg ctcggtgata gcggcactgg
     1441 gcggcgttga taacatctcg gcagtcacgc actgtatgac gcgtttgcgc tttgttatca
     1501 aagatgacca gcttatcgac agcccgacgt taaaaaccat ccccggcgtg ctcggcgtgg
     1561 tacgtagtga caaccagtgt caggtaatta tcggcaatac ggtttcgcag gcctttcagg
     1621 aagtcgtcag cctgctgccg ggagatatgc agcccgcaca gcccgtgggt aaacccaaac
     1681 tcacgctgcg tcgcattggt gcggggatcc tcgatgcgct gatcggcacc atgtcaccgc
     1741 tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga
     1801 gcggcgtgct gacaaaagga tcgccgacct taaccattct gaatgtgatt ggtgacggtg
     1861 ctttcttctt cctgccgcta atggtcgcgg catctgccgc catcaaattt aaaaccaata
     1921 tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc gagttttatt gaattaatgg
     1981 cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat cccggtcacc gcggtgaaat
     2041 acacctacac ggtgatcccg gcgctggtga tgacctggtg cctgtcatat atcgaacgct
     2101 gggtggatcg cattacaccg gcggtgacca aaaacttcct caagccaatg ctgattgtgt
     2161 tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg atcggtagcg
     2221 ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca gtcgccatta
     2281 tgggcgcgct gtggcctctg ctggtaatga ccgggatgca ccgcgtcttt acgccaacca
     2341 tcattcagac cattgccgaa accggcaaag aaggaatggt catgccatca gagatcggcg
     2401 ctaacctgtc gctgggcggt tcatcactgg cggtgccgtg gaaaacgaaa aacccggaac
     2461 tgcgccagac ggcgctggct gcggcggcat cagccattat ggcggggatt tccgaaccgg
     2521 cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt atcagcggtt
     2581 ttatttgcgg c
//
LOCUS       DADLTI010000293         2567 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLTI010000293 DADLTI010000000
VERSION     DADLTI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.297"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JN755_004208"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JN755_004208"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7382768.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>2567
                     /gene="cpdB"
                     /locus_tag="JN755_004209"
     CDS             943..>2567
                     /gene="cpdB"
                     /locus_tag="JN755_004209"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY7382769.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVAT"
BASE COUNT          660 a          639 c          652 g          616 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1261 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1321 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1381 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1441 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1501 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1561 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1621 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1681 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1741 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1801 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     1861 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     1921 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     1981 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2041 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2101 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2161 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2221 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2281 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2341 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2401 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2461 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2521 aatggcaaac cgattgatcc gaacgctatg ttcctggttg ccaccaa
//
LOCUS       DADLTI010000294         2548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLTI010000294 DADLTI010000000
VERSION     DADLTI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.298"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..444
                     /locus_tag="JN755_004210"
     CDS             <1..444
                     /locus_tag="JN755_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7382770.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="JN755_004211"
     CDS             445..810
                     /locus_tag="JN755_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7382771.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..1523
                     /locus_tag="JN755_004212"
                     /pseudo
     CDS             807..1523
                     /locus_tag="JN755_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
     gene            1717..1792
                     /locus_tag="JN755_004213"
     tRNA            1717..1792
                     /locus_tag="JN755_004213"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1750..1752,aa:Ile,seq:cat)
     gene            1794..1870
                     /locus_tag="JN755_004214"
     tRNA            1794..1870
                     /locus_tag="JN755_004214"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1828..1830,aa:Lys,seq:ttt)
     gene            1873..1948
                     /locus_tag="JN755_004215"
     tRNA            1873..1948
                     /locus_tag="JN755_004215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1906..1908,aa:Thr,seq:tgt)
     gene            1952..2026
                     /locus_tag="JN755_004216"
     tRNA            1952..2026
                     /locus_tag="JN755_004216"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Gly,seq:tcc)
     gene            2319..2534
                     /locus_tag="JN755_004217"
     CDS             2319..2534
                     /locus_tag="JN755_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7382772.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          675 a          552 c          680 g          641 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggttg
     1321 gtggggaagg ttatgaatac caggagctgg catttactgg cgggagtgac aactaaaaac
     1381 tggtccaaaa catttactcg tcactggatt gcaatgaaac acatttttca ccgactggat
     1441 agtgaggctt tattgtttgt aatgagaaca cgttcaaaaa aaagacggca ttttcaaagc
     1501 aaagtgttgc aaaagtagat tgaaaggcat atattcatgc aaatctgata ttttgccgat
     1561 tttttacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt tgtgccactt
     1621 atctcggata gacatggtga atgcgctggt ggaggagata agggtgattt ttgaatgctt
     1681 gcaacattga tttcgtaacg ttattatcct gcgcccggcc ctttagctca gtggtgagag
     1741 cgagcgactc ataatcgcca ggtcgctggt tcaaatccag caagggccac caaccgccac
     1801 tagctcatca ggaaagagcg tcaacccttt aagttgagtg tgcgaggttc gagtccccgg
     1861 tggcggtcca gtgccgactt ggctcagtag gtagagcaac tgacttgtaa tcagtaggtc
     1921 accagttcga ttccggtagt cggcaccata tgcgggcatc gtataatggc tattacctca
     1981 gccttccaag ctgatgatgc gggttcgatt cccgctgccc gctccagtta gagtctttca
     2041 gtctgcgatg atgggaaatc ccggagtgac tgaaagacgt ttaagttatg aatgatcgct
     2101 tttttttgca aaattgctgt gcagaaatac taaccttcgg gcaggcgatc attcataagc
     2161 actctgcttt tattccgatt aactgtgagt ggtttgttgg atagagtgct ttccttactg
     2221 tatatatcgt ttcgcccgct tttgcggttt tttcttttca aatccctttc atttctcagt
     2281 gtaaaactac gccatccgtt atttgcggag gtgaggctat gaaatccatg gacaaaattt
     2341 caacgggcat tgcctacggc acctccgcag gcagtgctgg ctactggttt ttacagtggc
     2401 ttgatcaggt cagtccgtca cagtgggctg cgattggtgt actggggagt ctggttctgg
     2461 gcttcctgac ttatctgaca aatctgtact tcaaaatcag agaagacaag cgtaaggctg
     2521 cgagaggtga ataatgtcgc catcatta
//
LOCUS       DADLTI010000295         2522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLTI010000295 DADLTI010000000
VERSION     DADLTI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.299"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JN755_004218"
     CDS             complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JN755_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY7382773.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(792..1193)
                     /locus_tag="JN755_004219"
                     /pseudo
     CDS             complement(792..1193)
                     /locus_tag="JN755_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1622..2410
                     /gene="virF"
                     /locus_tag="JN755_004220"
     CDS             1622..2410
                     /gene="virF"
                     /locus_tag="JN755_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7382774.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2352..>2522)
                     /locus_tag="JN755_004221"
     CDS             complement(2352..>2522)
                     /locus_tag="JN755_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7382775.1"
                     /translation="LQSLRSLSVIERNENIVLLGPSGVGKTHLAIAMGYEAFKIFYDI
                     SKISLELYHNIH"
BASE COUNT          767 a          460 c          471 g          824 t
ORIGIN      
        1 cccctaagcc gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat
       61 atatttctcc atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt
      121 ctactaaaag gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaacgcat
      181 gataaatata tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct
      241 gaaatagaat tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat
      301 atattatttt gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc
      361 gttggctttg tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg
      421 tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag
      481 ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacag
      541 gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt
      601 gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata
      661 gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg
      721 gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca
      781 gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa
      841 cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc
      901 aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac
      961 cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc
     1021 aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact
     1081 ggattggccg ttccggtcct ttatcgccat atcactcctg acatgacatc ctgcaccgtt
     1141 gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga
     1201 acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc
     1261 aacaccaacc ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga
     1321 tattatgatg ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg
     1381 cctaagaaaa gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg
     1441 ttgcacagag aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa
     1501 tataggaaaa attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt
     1561 acagaaattt cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt
     1621 tatgatggat atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat
     1681 tattttatat gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat
     1741 cgatgaaggg caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa
     1801 atctgatagc attaatccat ttgagattat aagccttgac agaaatttat tattaagcat
     1861 tattagaata atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg
     1921 gttaaacaaa aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc
     1981 tataaaagag atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc
     2041 tgtttctgat gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc
     2101 tgatcagata aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat
     2161 ttcaaataac ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt
     2221 aacatttcaa caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa
     2281 tagtcaaagc tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt
     2341 tataaggaaa tttaatgaat attatggtat aactccaaag aaattttact tatatcataa
     2401 aaaattttaa atgcttcata gcccatcgct attgccagat gggttttccc cacacctgat
     2461 ggccccagta atacgatatt ttcattacgt tctatgacgc tgagtgagcg taacgactgg
     2521 ag
//
LOCUS       DADLTI010000296         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLTI010000296 DADLTI010000000
VERSION     DADLTI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.300"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            155..1108
                     /locus_tag="JN755_004222"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JN755_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JN755_004223"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JN755_004223"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7382776.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          691 a          614 c          562 g          636 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gagtggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaagc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttac tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADLTI010000297         2489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLTI010000297 DADLTI010000000
VERSION     DADLTI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.301"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="JN755_004224"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="JN755_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7382777.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="JN755_004225"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="JN755_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7382778.1"
                     /translation="MSRFLICCFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..>2489)
                     /locus_tag="JN755_004226"
     CDS             complement(1928..>2489)
                     /locus_tag="JN755_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7382779.1"
                     /translation="VVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKS
                     FLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTF
                     LPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIP
                     AIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          713 a          663 c          636 g          477 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaacaacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca
     2161 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     2221 agttatcaat cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     2281 acgaactggc aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct
     2341 gtttggtgcc gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag gagattatcg
     2401 ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag agataaatca
     2461 ccagcgccag cagcgaacca aataccacg
//
LOCUS       DADLTI010000298         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLTI010000298 DADLTI010000000
VERSION     DADLTI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.302"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..564
                     /locus_tag="JN755_004227"
     CDS             <1..564
                     /locus_tag="JN755_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7382780.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            522..1199
                     /gene="bglJ"
                     /locus_tag="JN755_004228"
     CDS             522..1199
                     /gene="bglJ"
                     /locus_tag="JN755_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7382781.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JN755_004229"
     CDS             complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JN755_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7382782.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2166..2402
                     /locus_tag="JN755_004230"
     CDS             2166..2402
                     /locus_tag="JN755_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7382783.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          532 c          627 g          653 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc gcagggtgca
      481 agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac agccgaatta
      541 agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt attgagagtt
      601 tattcagaaa gtttgcgggc aacccttata agctccatac ctataccagt caggagtcat
      661 ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt tctgccatga
      721 gaagtgagcg gagagaggga ttatcttgcc tgactgagct ggcgattaag tttccgcgta
      781 cccggcgttt agttattgtg gatgatgata ttgaagctcg gctgattggt tcattgtcgc
      841 catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt catcaggaat
      901 tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat cagtggtaca
      961 ttaaccaaag ccggacgtta agcccgacgg agagagaaat attgcgcttt atgtcgcgtg
     1021 gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg atccgagcgc
     1081 ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg ttggaggccg
     1141 cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat ccctattaat
     1201 ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac aatagccaca
     1261 ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca gcaggccgtc
     1321 gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg ttttctcaaa
     1381 aaagagggca tggcgcagcg attcaacagt agcctcgccg agcagttgct tcatctcagt
     1441 gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt tgatctctcc
     1501 ggtcgcttct agtgcttgca caaccggaac cagcgcctgg ctgattaacg tttccattcg
     1561 ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc agaaacaggc
     1621 gacgcgtccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat ctaatgcttt
     1681 ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat accattgtgc
     1741 ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc gatagatatg
     1801 atcggaatag accgccagta gagaacttag cgcattcggt gatgaccatt gcgccagcgt
     1861 catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat gttcacgatg
     1921 tttggcgatc atcgcccgta cggcttgtgc aagcccagca tcctgcggct ggagatgcgt
     1981 tcgccagatg acatcttcat agagcggtgc ggaacgatag gccataatcg tgatagtaat
     2041 ctaaatgata atgattgcta atcctatcga taggtttact cgatagcaag ggatttctct
     2101 ggcttgcaaa tgataaaaat tatcatatga tattggtttt cattatcaat gaaagagatg
     2161 aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgccg ggattgctga
     2221 ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc attctgatgg
     2281 gattagtatt gacgccggtg atgctgtatc acaaacagtt gcggcattac attttgctac
     2341 catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg aatcagggat
     2401 gacaaaacag ggaagaaaca agaaaggaag taaagataa
//
LOCUS       DADLTI010000299         2436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLTI010000299 DADLTI010000000
VERSION     DADLTI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.303"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            30..1082
                     /locus_tag="JN755_004231"
     CDS             30..1082
                     /locus_tag="JN755_004231"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7382784.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1158..2436
                     /locus_tag="JN755_004232"
                     /pseudo
     CDS             1158..2436
                     /locus_tag="JN755_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          581 a          547 c          626 g          682 t
ORIGIN      
        1 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
       61 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
      121 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
      181 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
      241 ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc
      301 ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg
      361 cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg
      421 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
      481 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa
      541 ccggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
      601 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
      661 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
      721 ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
      781 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
      841 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
      901 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg
      961 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
     1021 tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct
     1081 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
     1141 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
     1201 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
     1261 tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg
     1321 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
     1381 cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct
     1441 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc
     1501 tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg
     1561 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
     1621 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
     1681 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
     1741 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
     1801 aaattagctg gggtggatgg gcgctggccg ccggattgcc ggattgccgg gcatcattat
     1861 gctgctggtc accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga
     1921 taacaaaacc atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa
     1981 aatgctgctc ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg
     2041 ggttgatgaa tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt
     2101 tacctgggaa gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg
     2161 tattatcggc ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt
     2221 ctttaaaaat aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt
     2281 cctcagcatc atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt
     2341 acctgtattt gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc
     2401 actgttgttc tccaactcct atggcggcat ggttac
//
LOCUS       DADLTI010000300         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLTI010000300 DADLTI010000000
VERSION     DADLTI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.304"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..823)
                     /locus_tag="JN755_004233"
     CDS             complement(<1..823)
                     /locus_tag="JN755_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7382785.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELCLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JN755_004234"
     CDS             complement(1424..1672)
                     /locus_tag="JN755_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7382786.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JN755_004235"
     CDS             complement(2027..>2373)
                     /locus_tag="JN755_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7382787.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          614 a          437 c          574 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcataat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tgattccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atacaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADLTI010000301         2331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLTI010000301 DADLTI010000000
VERSION     DADLTI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.305"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..156
                     /locus_tag="JN755_004236"
     CDS             <1..156
                     /locus_tag="JN755_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7382788.1"
                     /translation="TSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYI
                     RIQCLGG"
     gene            complement(245..757)
                     /locus_tag="JN755_004237"
     CDS             complement(245..757)
                     /locus_tag="JN755_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7382789.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            952..1716
                     /locus_tag="JN755_004238"
                     /pseudo
     CDS             952..1716
                     /locus_tag="JN755_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1667..>2331
                     /locus_tag="JN755_004239"
     CDS             1667..>2331
                     /locus_tag="JN755_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7382790.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          659 a          518 c          590 g          564 t
ORIGIN      
        1 acctccagcg cctccggcat gggcaatgat gccaccgtcg aactctctcc agttgctgga
       61 cgaaacgttc tcggtatccg ggacggaatc atcagcgacc agacagcatt gagaatgctt
      121 caggagtaca tcaggatcca atgccttggg gggtagcggt aattttactc attatccttc
      181 aaatcagatt ctgttgtcag aggaatgggg gaagctggat tcgcaagagc tttttatggg
      241 cagtttagct aaaaaaaggc aacacgaagc taaaactacc aacataaagt gctgaccagc
      301 tagcagctga aatcagactc actgtataca ctttcactgg atgaagttgc aacattcctg
      361 ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg cgaagtgaaa agtattgaca
      421 cagagttatt ttgcaactga aggcgaaccc gttctattgt attggaacgg ctcgatatta
      481 tacatgcaag ttgagggata tgcccaatta ttataccaag atggtaatta acgatagtac
      541 cgcaccatgc gccactcatg accgtgattc cagcttccca tggtgaaaga gtcgtcttgc
      601 tgactgtaat tacagtggcc atcataattg atgctggtgg caatatcgcc gaaatgagta
      661 aagtggattt tgtgaacgct attataaaaa gaagccccca taggcgatat ggatgaagta
      721 caaaatagtt catcaaagca tttatccact ccatcaatgt ctttccttct gtgaattttt
      781 ctttgctatt taaccgtgat acgacttaat ttaggcttaa acaccttaca tttacaaggt
      841 actcctgtgg ggggccctgc cacggagcgt cgcaggagtg agtgatgagg aagaattaaa
      901 gcaacctatt gtgacaggca acgataacag taaatcacag gaagcataat catggcaaaa
      961 ccggactggg aggccatcga atcggcatac cgggccggag tccttagcct ccgtgatata
     1021 ggcgagaaat acggcgttac agaaggggct atcaggaaga gggccaaaaa gcttggttgg
     1081 gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa agtgcgtacc
     1141 agcagaaagc ctgccattac tggccttaca caaaaaagta cgcaactaaa aacagaatct
     1201 acaccggata cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa cccattccaa
     1261 cccggtaacc agcaggcatt aaaacacggt ggttatgccc gtcgccttct gctcaaagat
     1321 gaggtgatag aggacgctaa agcgttgacg ctcgaggacg aattatttcg ccttcgtgct
     1381 aacaaccttg tcgctgcaga gaatattggt cggtggttgg tgtcgctgga agatgctaat
     1441 ggggactagg aaaggaagat gctgattgaa aatatcagcg ccgccgagaa agcaatgatg
     1501 cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg aaaaatattt
     1561 gctgatacag cctaccgcaa ggccgccact gataaggtgt ctctggaggc tgatcgtctt
     1621 cgccgtgatg caggtattga tgatggcaat ggagagcgtg acctcaatga cttctactct
     1681 gacatccaaa ccgacgctga atccggcttt acgtagtttc tggactatgc gggcacgtaa
     1741 caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg gcattgccat
     1801 atttctgtcg aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc agaataaaat
     1861 cgaagagtcg gtgtataccc tgctcaaaat tcagatagac aggtttggtc tgcggcaccg
     1921 tttccgtatt ctgaacaaca aaatcattaa ccgggttact ggctcggaat ttgtttttta
     1981 tggattatgg cgcaacatcg aagaaattaa gtcactggag gggatcgatg tgttgtggct
     2041 ggaagaagcc cacgcactga cggaatacca gtggaaaatt ctggagccaa cgatccgtaa
     2101 agagggttcg gaatgctggt tcatattcaa ccccggactt gttactgatt tcgtctggcg
     2161 caacttcgtt gttgatccgc ccgaaggcac tctcatccgc aaaattaact atgacgaaaa
     2221 tccgtttctg tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc gtgatccgga
     2281 tggttttaaa catgtgtatg agggcgttcc ggagtctgat gatgatgcgg c
//
LOCUS       DADLTI010000302         2301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLTI010000302 DADLTI010000000
VERSION     DADLTI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.306"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..785)
                     /locus_tag="JN755_004240"
     CDS             complement(<1..785)
                     /locus_tag="JN755_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7382791.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1133..2005
                     /gene="rluF"
                     /locus_tag="JN755_004241"
     CDS             1133..2005
                     /gene="rluF"
                     /locus_tag="JN755_004241"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7382792.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2138..>2301)
                     /locus_tag="JN755_004242"
     CDS             complement(2138..>2301)
                     /locus_tag="JN755_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7382793.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWS
                     ANTSTRGRR"
BASE COUNT          562 a          588 c          577 g          574 t
ORIGIN      
        1 tcgctcatat tcgtttcaac cattgcgccg tgaatatcaa aaaagcgcga gcaaatatcg
       61 ccagcaacct ggcgggcatt caggtcgtca ctctcttcac caccataaaa cgcatgccag
      121 ttagcgccgt cgcgaatggc cggtgagcca attcccacca gggcgacatc cagattatcc
      181 cagtaggcag agatggtttt aaagtgctga gactgcatga tcccattacg aattaatggg
      241 ttatccagca gagccggaaa atccgcgaga tgcgattccc ctttcagctt cgctgccgcg
      301 ctgtaggtta atgtgtttac atgatagcgg ctttcgagtt taccggacgg tccgccaata
      361 attggtacgc agattaactg ccgcgattgc cccgcctgcg gcaagttttc aaccagtgcg
      421 ctcaccgcgc gaccccagga aaaaccgaca atatcgccag gctccagcaa gcgatccagc
      481 agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct cttcatcctc atcatttccc
      541 gacaccacca caacgtcttt caggccaaac ttttgcttca gttgctgctc cagccagaga
      601 ttttcgttgt agtcatagtt gatggcgatg gtgaccattc cctgatcgcg gcctcgttta
      661 agcaagcggc tgatggtggt gcggtaaata cccagttcgc gcgcgatttg cgcctgcgtc
      721 atatcctgtt cgtaatagag ttgggcaatc ttcacaatca aacggatatc gtcactgttt
      781 tccattgccg cctcctggtt ctgcacattt gtgcaaatac gctttctcac cgcttgatct
      841 gatataaccc catcatgagg gcctttcaaa gcgatttttc actctttgca tggggagaaa
      901 tagcgctttc tcttgcacat ttgtttctct gcgtagtagt catcagcaca ggtgtgcagc
      961 gattcgtaat atgttgatta tccttatttt gccaggcaaa tttgcatcgt acttccggag
     1021 acacttttgt gataaaaggc gtggcagatc acatttaatt tcgacgttgc cgcataaccc
     1081 tgtttttccc cgctaagagg tacggtatat tccgcgtttt acaggagaat ttatgctgcc
     1141 cgactcatca gtccgtttaa ataaatacat cagcgaaagc ggaatttgct cacgccgcga
     1201 agcggatcgc tatatcgagc aaggcaatgt gttccttaat ggcaagcgag ccaccattgg
     1261 cgatcaggtg aaacccggcg acattgtgaa agtaaacggt cagttgattg agcctcggga
     1321 agccgaagat ttggtactta tcgccctgaa caagcccgtc ggtattgtca gcaccaccga
     1381 agatggcgag cgcgataaca ttgtcgattt cgttaaccac agcaaacgcg tgttcccgat
     1441 tggccgcctg gataaagact cccaggggct gattttcctc accaatcacg gcgatctggt
     1501 gaataagatc ctccgtgcgg gtaacgatca tgagaaagag tatctggtga cggtcgataa
     1561 accgattacc gatgaattta ttcgtggtat gggggcgggg gtgccgatcc tcgggacggt
     1621 gacgaaaaag tgcaaagtca aaaaagaagc gccgtttgtc ttccgcatta ccctagtgca
     1681 ggggctgaac cgtcagatcc ggcgcatgtg cgagcatttc ggctatgaag tgaaaaagct
     1741 ggaacgtacg cgcattatga acgtcagctt aagcggcatt ccgctggggg agtggcgcga
     1801 tttaaccgac gatgagttaa tcgacctctt taagctcatc gaaaattcct cttccgaggc
     1861 aaaacctaaa gcgaaggcca aaccgaaaac agcgggcatc aaacgtccag tcgttaagat
     1921 ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac ggtaagcgtt ttacctcgcc
     1981 ggggcgtaaa aagaagggac gctgattaac gcctcagact gatgaaaacg tccaaaaaga
     2041 gctcggacag tcccctcgcc ccttcgggga gagggttagg gtgaggggaa gattgtgtcc
     2101 acacagacga gtgaaaatct caggcgttga ttgttcatta gcgcctgccg cgcgtcgacg
     2161 tatttgctga ccagctaaat gacgcttcag gatccggttt ataagcctgc tttttcttca
     2221 attggcgggc ttttttcgct tcagcctcac gctcgaccat taatttatcg atgtactctt
     2281 tcttaatgct gttggtttcg g
//
LOCUS       DADLTI010000303         2258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLTI010000303 DADLTI010000000
VERSION     DADLTI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.307"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            61..>2258
                     /locus_tag="JN755_004243"
     rRNA            61..>2258
                     /locus_tag="JN755_004243"
                     /product="23S ribosomal RNA"
BASE COUNT          620 a          490 c          691 g          457 t
ORIGIN      
        1 agttgttcgt gagtctctca aattttcgca acacgatgat gagtcgaaag aaacatcttc
       61 gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg
      121 aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga
      181 tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt
      241 aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga
      301 gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg
      361 ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc
      421 acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg
      481 accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa
      541 aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc
      601 agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat
      661 attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg
      721 ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg
      781 ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg
      841 ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg
      901 tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg
      961 acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc
     1021 gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag
     1081 tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag
     1141 cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg
     1201 taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg
     1261 ggagcgttct gtaagcctgt gaaggtgtac tgtgaggtat gctggaggta tcagaagtgc
     1321 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
     1381 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
     1441 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
     1501 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
     1561 gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca
     1621 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
     1681 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
     1741 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg
     1801 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
     1861 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
     1921 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
     1981 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
     2041 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
     2101 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
     2161 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
     2221 gcatggagcc gaccttgaaa taccaccctt taatgttt
//
LOCUS       DADLTI010000304         2152 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLTI010000304 DADLTI010000000
VERSION     DADLTI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.308"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            83..463
                     /locus_tag="JN755_004244"
     CDS             83..463
                     /locus_tag="JN755_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7382794.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(504..1454)
                     /gene="corA"
                     /locus_tag="JN755_004245"
     CDS             complement(504..1454)
                     /gene="corA"
                     /locus_tag="JN755_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7382795.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1824..>2152
                     /locus_tag="JN755_004246"
     CDS             1824..>2152
                     /locus_tag="JN755_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382796.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQR"
BASE COUNT          580 a          506 c          511 g          555 t
ORIGIN      
        1 tttttgattt gttatttttc cactttcagt atgctaatgc ttgcacctac tgattcatca
       61 aaacaatagt tatggagtat ttatgagtaa ggaatatatg aacgatgatt cattaagtga
      121 aaaatggaaa taccgattta atttttatga tcaacatggc tttcctggat tctggggagc
      181 aacaccagaa tataaagcag catttaaagc attaaaagta agacaacgat taactattca
      241 aatgaatttc attgctttct tctgttcctg gatctattta tttgttctgg gcttatggaa
      301 aaaagcaatt atagttctat tacttggcat tctatctcta tttgttggtg cattaattgg
      361 cgttaacatt ctgggaattg ccgtagcagc ctatgttgct gttaacacga acaaatggtt
      421 ttatgagaaa gaggtaaaag ggttaaatac ctggagcctt tgaataacag gtggcttagc
      481 caggctaagc caccgccttc tttttacaac cagttcttcc gcttaaagta cagatacggt
      541 gccaggcccg cgaggatcat aaagataatc gcgccagggt agccgaaact ccacttcagt
      601 tctggcataa actcaaagtt catcccgtag ctggaagcaa ccagcgttgg cggcaggaac
      661 acgacggata ccaccgagaa aattttgatg atgcggttct gctcgatgtt gataaaaccc
      721 attgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg cggcagcagg
      781 gattcgatat cgcgcaggat ttcacgcgcc tgctccagtt gcccacccgg taaacgcgct
      841 ttacgcacca ggaagttgag agcgcgctgg gtatccatca gacacaggcg aactttccag
      901 ccgatatctt ccagttccgc cagcgtggag agcgcctcgt cgtactcatc gccctgatgc
      961 ccttccataa tcacccggct caactgctcc aggtcgctat agatattttc aatttcatct
     1021 gccaactgtt cgattttggt ttcgaacaga tccagcagca actcgtaggc gttaccgtct
     1081 accatcgact ggctacgggc acgcatacga tacagacgaa aagcgggcag ttcacgctca
     1141 cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt accggcgtga
     1201 tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcgtc gtcttcaaag
     1261 aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct ctggccaagt
     1321 tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat ccatactgca
     1381 tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt ttccagttga
     1441 aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt cggtgggcac
     1501 acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac gggaaaaatt
     1561 gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg agttgatcct
     1621 ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg tcctcttaga
     1681 gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa cagaacacca
     1741 cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa caggataaaa
     1801 aaatcccaca tccaggacct ataatggcgt atcggctact cattggtaaa ggaatgatca
     1861 ccttgaatct caaacggatt tttctcaccc tcaccttact tcccttgttt gctgttgccg
     1921 ctgatgattg cgcactctca gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc
     1981 aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac
     2041 atgctctgct ggcggatatt aatagtcagg gtcaggtgca gatggcgatg aacggcgaca
     2101 tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag cg
//
LOCUS       DADLTI010000305         2151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLTI010000305 DADLTI010000000
VERSION     DADLTI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.309"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JN755_004247"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JN755_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7382797.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JN755_004248"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JN755_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JN755_004249"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JN755_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JN755_004250"
     CDS             1905..>2151
                     /locus_tag="JN755_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382798.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          634 a          395 c          490 g          632 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaacacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt accgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagta ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADLTI010000306         2141 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLTI010000306 DADLTI010000000
VERSION     DADLTI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.310"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            324..577
                     /locus_tag="JN755_004251"
                     /pseudo
     CDS             324..577
                     /locus_tag="JN755_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            734..2137
                     /locus_tag="JN755_004252"
     CDS             734..2137
                     /locus_tag="JN755_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382799.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFA
                     IIFTLMSRERSLEFEKE"
BASE COUNT          556 a          422 c          490 g          673 t
ORIGIN      
        1 tatctgcgac ccagacctga ttaggggggt tggggagcaa tggaacagta aacgccgtta
       61 agaagtcaat ttaaatagcc aaaaattaac tagccaattt acattgcagg ttgtaatttt
      121 taaccacctt ttttacgata gccaaattac ctatacccat cccggccctg ccaagccata
      181 cccgctatcg gcgatgaaaa tgcttttgtg cgcggtcgga gtggtcccga cgagggtttt
      241 acccgaagtc ggggcgtatc tccgcgttag cgggccgtga gggccgctta cgagcgtgta
      301 tgagagacgt acagtggttt tgtccgctca caccagtaca tacaggtacg gaaataaaac
      361 cagtcgaaat gccggtaacg acgattactc ctgtctctga tgccggtgga ctacaagatt
      421 ttatttactg gcgtcctgat gcggacagga ctggtatgga acctgtttat gtgatactta
      481 atgatcctct ggattccggg cgatttacgc gtaaacaact aaataaaaat atcttaaaca
      541 tgcaagtaat tttggtattc tcgatacaag aataaatagt agtaactact atagttgcaa
      601 ataatgttaa aaacatttct atgatcatct ttacattttt catgattgac aattgttttt
      661 ggttaaaaaa agacaactaa tgagtgtgat aatgcaatac tttgagtaat tatataatga
      721 taagtaaaca ggtttgcgac aattggttat cagtattaca gaaggattaa atatgtcatt
      781 attcacagaa cctaaggaaa ttgaaagatt accctctgaa gagattgaac gattatatcc
      841 cgtcctgcga tacagggtct ttatcagcat tttcctcgga tatatggggt attattttgt
      901 ccgaaacacc accagtgtgt tatccggtgt gctgcacatg tctgcaaccg agatcggcat
      961 tatctcctgt gccggttttt tatcctatgg tatatcaaag tttgtttccg gacttatttc
     1021 tgacagaagt aattcaaaag tatttttgtc tcttggttta ttcctgtccg gactggtaaa
     1081 ttttctgata ggatatattc ccgggatcat cacatccgtt acgttatttt caacaatgta
     1141 tttacttaac ggctggatcc aaggtatggg atatcctcct ggtgccaaaa cactggtgtt
     1201 ctggtatgaa catcgggaaa ggatcacttg ggccacactg tggaatttat cgcacaatgt
     1261 cgggggagct ctggcccccg ttctgattgg tttcagtttt ggttttttcg gcgacagtgc
     1321 tctggagcat gcccgggccg catttatctt cccaggagta ctgtgtatgg caatgagtgt
     1381 gctgatttac tttattcagg tggacagacc tgtatcagtg gggcttcctc ccattgaaga
     1441 atggaaaggt aacgtcgttt cacacccggc taaaggccgt gaacaaggtc cgagactctc
     1501 cattcctgat atcatcagaa aacatatcat ccgaaataat aaactcattt actgctgtat
     1561 ctatggttcg tttgtttata ttctgagata cggtattgtt tcctgggctc ctaagttttt
     1621 aagtgacagt ctggatgtcg gtggtaagga tatgggaaaa ctggcatcaa tgggggggtt
     1681 ttctgttttt gaaatcggtg gtgtggctgg tatgcttctt gccggatacc tgtctgtcag
     1741 actgttcaga aactcaaaac cattaacaaa tactttattt ctggcactta ccattatttt
     1801 attaatcgcg tactggtacg tgccatcagg caatgaatat ctctggctaa actatacaat
     1861 ccttattctg ttggggctgg cagtgtatgg tccggtgatg tttattggtc tttattcaat
     1921 ggagcttgtt cctaaagaag ctgccggagc cgcatcaggg ctgtcaggca cattttccta
     1981 tatcttcggc tcaattgttg cgacactggg aatgggactt gttgtcgatt atctgggatg
     2041 gggggccaca tttatcgtac tgatactgtc tgctgttttt gccatcattt tcacactgat
     2101 gagtcgtgaa cgctcactgg agttcgaaaa agagtaattt t
//
LOCUS       DADLTI010000307         2092 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLTI010000307 DADLTI010000000
VERSION     DADLTI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.311"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..185)
                     /locus_tag="JN755_004253"
     CDS             complement(<1..185)
                     /locus_tag="JN755_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382800.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..1213
                     /locus_tag="JN755_004254"
     CDS             371..1213
                     /locus_tag="JN755_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7382801.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1216..1821
                     /gene="capU"
                     /locus_tag="JN755_004255"
                     /pseudo
     CDS             1216..1821
                     /gene="capU"
                     /locus_tag="JN755_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
     gene            1878..>2092
                     /locus_tag="JN755_004256"
     CDS             1878..>2092
                     /locus_tag="JN755_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7382802.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATAR"
BASE COUNT          586 a          412 c          465 g          629 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga aaagacgact
     1141 tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg ggccaagatt
     1201 accggatgac taataatgaa tatactattt acggaatcat caccgaatat aggtggtcag
     1261 gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc agttctgctt
     1321 gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat tgatatcaca
     1381 ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact cggaatagtt
     1441 catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa tattgttggt
     1501 ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa gacatatttg
     1561 acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat tgttcccgga
     1621 acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct
     1681 cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc tccgagtgtt
     1741 ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc
     1801 ccggaaaaag ggcacgaatt taggtgatgc tgccaactta ctgatttagt gtatgatggt
     1861 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     1921 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact
     1981 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
     2041 aaatcattga tatggccatg aatggcgttg gatgccgggc aaccgcccgc at
//
LOCUS       DADLTI010000308         2076 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLTI010000308 DADLTI010000000
VERSION     DADLTI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.312"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(194..427)
                     /locus_tag="JN755_004257"
                     /pseudo
     CDS             complement(194..427)
                     /locus_tag="JN755_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(523..2046)
                     /locus_tag="JN755_004258"
     CDS             complement(523..2046)
                     /locus_tag="JN755_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7382803.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          621 a          459 c          442 g          554 t
ORIGIN      
        1 acatcaaaat tattaatagt tgccatggta attatttcgg tgtatataaa aacaactgtg
       61 ataacaccaa ggttgacaac tatttgcgca ataattcctt tccttcaatg atccaggcgt
      121 tcactgccag caacccattc agccgcatac tgacagtgag aaactgaaag attattgctg
      181 atccctgtgg ctgacgagtc tttctcttaa atcgattaac cagattgatc gccagccaaa
      241 caagggcatt aagcaaattt atcgtcacaa gcacttctcc cagcccggta ctgggttttg
      301 caataaggat gaccaggagg gtgatcagca taaatgcaat ggaaaagatg atatagagag
      361 tgaaataatc cagattatta ctattgttca tgctactaat ggcaatcctg tcaaaacttt
      421 caaacaatta tagcaaacaa aataatgaac aatagtaaca agaggcaacg agctaattag
      481 ctgtctataa ttacaccgca ctatggcctt actgatcaat tttcactcaa ccacaacctt
      541 caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag gtttatagaa
      601 ataataaccc tgctgaaatg tgatattatt ttggttcaga tagccaagtt gttctttcgt
      661 ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca atacacaatc
      721 cagaattcgg gttgattctg ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt
      781 gatataatca atatgcaggt catgaagata agaaagccct gagtagccag taccgaaatc
      841 atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa ccagactttc
      901 atcaacattc aatggctcac gctcggtgac ttccagaaca aggtttaaat cgcggcgggt
      961 aaaactgtca cggaaattta aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc
     1021 gctaaaatta attccaatat gaaaaccttc cggcagttta ctcgcgatag cgttcatctg
     1081 tctggcaacc tgattcatca ggctttgtgt aagcggaacg attaatccgg atttttcagc
     1141 aagtggaata aatgccgcgg gtgaaatata tccaccgtga ggttgtttcc agcgggctaa
     1201 cacctcaact ccccgcaatg tcccttcccg accatttacc acaggttggt aaaaagggac
     1261 gatctcccca ttatttattg cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa
     1321 atatctatca agcaggaagg cagcggcgca ggcgattaac agaataaata ttaaaatccc
     1381 aaagccttta tcgataagtc tgtagaagct aaagagtggc ggcgggttaa agataatagt
     1441 gaaatgatat tttgtggagt gaaccttctc ggtataggga taatgtccat taagcgtcat
     1501 cacatcaccc gttggtccaa taatgtcatc cgccacgcgt aatacatacc ttaccccttt
     1561 caagggtaca ttaagcgccc cgcgaatatg ttgatcactt atcgtaacca aaatgcgcgt
     1621 atctgcaaat tggctctgat agagcaggat aggtaatcta ttaacggtgt cgattgccgg
     1681 agccaacagt aaattactgt ccgggacaac aggaatacga gacagcagga cccgattccc
     1741 aggcagggat gtacaccaga ctattccctg tttgagaatg atgattgtgc gcaggtgagg
     1801 tttcagagct gcttcagtgc caagctgata ttgctcctcg aggtcgcatt ccttcccggc
     1861 aatgttaacc gctgtttgag tcgcctgtga cgcttcttca agtatatgat tgatattgtt
     1921 ggcggcatat cttgctcccg ccaggtactc tgcttttgcg gaataccaga gctggatgtt
     1981 caaaatagcg acaccggtaa taaacaagca tatcgcaacg aggatgggta tgagtgtatt
     2041 gcgcatttgc ttgtcccggc caaagggttt aataca
//
LOCUS       DADLTI010000309         2066 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLTI010000309 DADLTI010000000
VERSION     DADLTI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.313"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..692
                     /locus_tag="JN755_004259"
     CDS             <1..692
                     /locus_tag="JN755_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382804.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            794..>2066
                     /gene="amn"
                     /locus_tag="JN755_004260"
     CDS             794..>2066
                     /gene="amn"
                     /locus_tag="JN755_004260"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7382805.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVSYGTLL"
BASE COUNT          484 a          510 c          495 g          577 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctgtta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcatcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgc cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaaatatat
      901 tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg tctatccatc
      961 actgaccgta acctgggacg gtagcacaac aaatcccccc aaaacgcgcg catttggtcg
     1021 ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
     1081 tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct ctgtgcaacc
     1141 ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
     1201 ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc aaatcggcga
     1261 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
     1321 gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc cagtagaaca
     1381 ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
     1441 gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg
     1501 gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     1561 aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta ttactctggt
     1621 gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg cagtgctacg
     1681 cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagcc aggccattgg
     1741 agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc
     1801 gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg acgctaccaa
     1861 gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt
     1921 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     1981 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     2041 ccgcgtgtca tacgggacac tactgt
//
LOCUS       DADLTI010000310         2058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLTI010000310 DADLTI010000000
VERSION     DADLTI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.314"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..606
                     /locus_tag="JN755_004261"
     CDS             <1..606
                     /locus_tag="JN755_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7382806.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            725..1058
                     /locus_tag="JN755_004262"
                     /pseudo
     CDS             725..1058
                     /locus_tag="JN755_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          576 a          453 c          505 g          524 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     1801 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     1861 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     1921 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     1981 gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca tctctatggt
     2041 catcgcagac cactaacc
//
LOCUS       DADLTI010000311         2050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLTI010000311 DADLTI010000000
VERSION     DADLTI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.315"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..168
                     /locus_tag="JN755_004263"
     CDS             <1..168
                     /locus_tag="JN755_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7382807.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="JN755_004264"
     CDS             153..791
                     /locus_tag="JN755_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7382808.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1444
                     /locus_tag="JN755_004265"
     CDS             1019..1444
                     /locus_tag="JN755_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382809.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFGRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGEMNPMQCPG"
     gene            1432..1906
                     /locus_tag="JN755_004266"
                     /pseudo
     CDS             1432..1906
                     /locus_tag="JN755_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          619 a          366 c          482 g          583 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttggc agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagtggggg gggggagatg aaccctatgc aatgtcctgg
     1441 ttaatatctc agtgtgctca tcagtgcact gataataaaa agacggagac agatgccatt
     1501 tacgataaag ttcggagttc ttatttgctt tcatgtattc tgaaaaaaaa caaaaatgtt
     1561 gggcttattc ttcatgcacc atcatttgtt tctgtgtctg aaaaaaatcg ccagaattgt
     1621 gatggcaaat tactcccgta actggagtaa ttccgagttg gccagtgcgg ttttaatgag
     1681 tgaatcttct ttaaaaagaa gaatgtacaa agaggtcggt tctataagta catttgtaca
     1741 taaaataaag cttacagaag ctatcaggaa attacgaagg acaaatactc ccatttctgt
     1801 catatcatct gagttaggtt acagttcacc atcgtatttt tctaaagttt tctttaaata
     1861 tttaaagaca taccctcaaa acataaggaa gaaaaatggt cggtaatgtc accaattagg
     1921 aggacttgca gagccgcttt tttacgatac tgagatcaaa cagacataat ctatgcggag
     1981 atttacagca gggcatggtt cacaaatctg acagcgatga attatcggca ctgcgggcag
     2041 aaaatgctcg
//
LOCUS       DADLTI010000312         2042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLTI010000312 DADLTI010000000
VERSION     DADLTI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.316"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(220..384)
                     /locus_tag="JN755_004267"
     CDS             complement(220..384)
                     /locus_tag="JN755_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382810.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="JN755_004268"
     CDS             1286..1570
                     /locus_tag="JN755_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7382811.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..>2042
                     /locus_tag="JN755_004269"
     CDS             1558..>2042
                     /locus_tag="JN755_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7382812.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          527 a          427 c          584 g          504 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggttccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 ggtgaagctg tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtatcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 ta
//
LOCUS       DADLTI010000313         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLTI010000313 DADLTI010000000
VERSION     DADLTI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.317"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..362)
                     /locus_tag="JN755_004270"
     CDS             complement(<1..362)
                     /locus_tag="JN755_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382813.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JN755_004271"
     CDS             complement(429..1643)
                     /locus_tag="JN755_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7382814.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          632 a          493 c          414 g          493 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcgattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADLTI010000314         2018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLTI010000314 DADLTI010000000
VERSION     DADLTI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.318"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..188
                     /locus_tag="JN755_004272"
     CDS             <1..188
                     /locus_tag="JN755_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382815.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JN755_004273"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JN755_004273"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7382816.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..>2018
                     /locus_tag="JN755_004274"
     CDS             1156..>2018
                     /locus_tag="JN755_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7382817.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGT"
BASE COUNT          481 a          539 c          517 g          481 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcac
//
LOCUS       DADLTI010000315         2000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLTI010000315 DADLTI010000000
VERSION     DADLTI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.319"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            223..882
                     /locus_tag="JN755_004275"
     CDS             223..882
                     /locus_tag="JN755_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7382818.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="JN755_004276"
     CDS             complement(1083..1460)
                     /locus_tag="JN755_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7382819.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..>2000)
                     /locus_tag="JN755_004277"
     CDS             complement(1527..>2000)
                     /locus_tag="JN755_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382820.1"
                     /translation="LMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRV
                     HAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERA
                     AHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          493 a          493 c          516 g          498 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag
//
LOCUS       DADLTI010000316         1993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLTI010000316 DADLTI010000000
VERSION     DADLTI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.320"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN755_004278"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN755_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7382821.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1993
                     /locus_tag="JN755_004279"
     CDS             1494..>1993
                     /locus_tag="JN755_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7382822.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLLRCTG"
BASE COUNT          565 a          342 c          484 g          602 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtcttgt
     1981 tgaggtgtac tgg
//
LOCUS       DADLTI010000317         1963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLTI010000317 DADLTI010000000
VERSION     DADLTI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.321"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..1178)
                     /locus_tag="JN755_004280"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="JN755_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1829)
                     /locus_tag="JN755_004281"
                     /pseudo
     CDS             complement(1388..1829)
                     /locus_tag="JN755_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069642.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          459 a          514 c          499 g          491 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgagttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgcttata acgatcaata
     1261 taatagaaat gatcgtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tccggtccac cgcaccggta aactgttcac gggtctgagt gcggacagac
     1561 tccgtgcgca gttcgtcctg caggcgtgtc atcagaggga tggcggcatg tccgggaagc
     1621 actgtcatga gtgcactgac cggggtgacc tccccctgaa acagtgcatt acagagtgag
     1681 gatttaccgg ccccgctttt acccataatg ccaggcacgg gttcgtgacg ggggagtttg
     1741 cgcagatgtt ccaggatgtg acgggaaatc gggtaaggca gggaggagag cggtttttca
     1801 attgcctcaa tggcatcaga cggattcata catgtattcc cagtgaaaaa agacaaaacc
     1861 ccgtgcaccg gaaaggtggc gggggattca cggagataat atctttctga aattattttg
     1921 aatgcaggta tatgtggctg atttgtatca gctaatgagt aca
//
LOCUS       DADLTI010000318         1946 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLTI010000318 DADLTI010000000
VERSION     DADLTI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.323"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            134..625
                     /locus_tag="JN755_004282"
     CDS             134..625
                     /locus_tag="JN755_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382823.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            709..1698
                     /locus_tag="JN755_004283"
     CDS             709..1698
                     /locus_tag="JN755_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382824.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          481 a          513 c          482 g          470 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcgctaaaga
      421 gctagcgtct cagaaagagc gtacccgcga aatgttcatg accgaagacg aatggcaaaa
      481 agagctacag cgccgcgaac aggtcaaagc tgaacgccgg acctatggtg aaaacctccg
      541 cagcgctact cattccgctg gtcgctcacg tgccgctatc gtcgctgacc ttgagtcagg
      601 cggtaactgg atggacagct tgtaagcaag cgtccctcat ctgagaacac acggtccctg
      661 actgcctgtt agggacttca agtatttgac tcaaggagat taattataat gaatatgact
      721 aaaaacgctt tccagtttgg cattgagcct gttcatatca ccgacaacga caaccttcag
      781 gttaatgagg gccttccgac taacgccgac cctcaggtct atgccctcca gttagctaag
      841 actgttaagg ctatgctgaa cggtgtactt aaagacgctc aagataacat cccgttccct
      901 gtagaagtcc tgccgacccg taacagctta ccgactccaa tcattgctca cactctggct
      961 gaccgctctg tgctggtccc tgtccgtggt ggtaagcgac ctgaagttgt gaccgctccg
     1021 tctggcgctg aaatcaccgt tgagccgatt gaacaggcga tcctcgtgtc tcaccaaaca
     1081 aagctttggg accagaagtc gaccactggt ttcactcagg ggacgctcca gcaggacgca
     1141 ctgaacatct gcgataacgt gattcgtacc attaactcta agatggttga cgtgctggag
     1201 tcctcaaagc tgctgaagac cgtagaactt ccggccctga ctggtagcct gaccgctaag
     1261 gctgacgcta tcatggatgc cctgtacgag aacacggagt cttctttcgg ttctgaggtt
     1321 tctgactacg gcattatcgc ccatgagtca cacctcaagg ctctctcccg tctggctgct
     1381 aagcagggct tcggtggtga agacgctatc gtcgatatgc tggggactga cgtcgcctat
     1441 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
     1501 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
     1561 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
     1621 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
     1681 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
     1741 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
     1801 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
     1861 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
     1921 caaaagcccc catagcctga cctgag
//
LOCUS       DADLTI010000319         1918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLTI010000319 DADLTI010000000
VERSION     DADLTI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.324"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JN755_004284"
     CDS             complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JN755_004284"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7382825.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          481 a          445 c          470 g          522 t
ORIGIN      
        1 attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat
       61 atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg
      121 ccgctcggta ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg
      181 ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta
      241 accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg
      301 gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg
      361 ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca
      421 cggcataccg cttcggtgat gcgcagttta cggttgtcat tgttgaactc gagacggctg
      481 ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg
      541 tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg
      601 tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc
      661 atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc
      721 tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg
      781 atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt
      841 ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata
      901 acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttt ttcgcaacca
      961 aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc
     1021 aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg
     1081 ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg
     1141 ccaacagaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg
     1201 ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacatcgtta
     1261 ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg
     1321 gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca
     1381 tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct
     1441 aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg
     1501 acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg
     1561 gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga
     1621 aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag
     1681 tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat
     1741 acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt
     1801 agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg
     1861 ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag gataagtgaa agttcatc
//
LOCUS       DADLTI010000320         1896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLTI010000320 DADLTI010000000
VERSION     DADLTI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.325"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(106..1560)
                     /gene="ospC2"
                     /locus_tag="JN755_004285"
     CDS             complement(106..1560)
                     /gene="ospC2"
                     /locus_tag="JN755_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7382826.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          605 a          321 c          293 g          677 t
ORIGIN      
        1 tgcaatgtgt tgttgcaggg gaacggtctg caccatcccc tatatcgatc aatataacaa
       61 aaatgacaac gtcagattca gggcacaacc taaatatatt ttaggtcacc tcccaaatcg
      121 tttgcctgat accgctccat tttttaataa aaaatcaatt gtttttgaat ctttgctttt
      181 cattgcattg tcaagcatag tgtccccact atttgctttt tgaaatcttt tgtttacatc
      241 aaccaatcca ttattaataa aatattctag cactttatag ttggcactat cttcactaag
      301 taaatactca acatcataca gctcacaata catattgttg ttcattttct ccatctctgc
      361 gacatcttgt tttgtaaaat taaatttaga tagtaaataa agtgccatat cagattttgc
      421 attgcctaga gaataagcaa gagcttgatg agccattttt ttatcagtca ccttattgtt
      481 aatttcttca taattggatg ctttaatggc atcctccaaa gatatatctc tgagtcttat
      541 ctttttgaag ttatctgtgc ttagcattct tggtatatga tactcaagct gaaacacaaa
      601 gtttataatt ctatccagac taacaggatc aatgttttta ttataacaaa actctcggaa
      661 tctttgatct ttactctttc ttataaaatc aataagatgc aacccaattc caagcctcat
      721 atcttttgta ttaaatattg gcacatcagt ttttccctga ttaccatgca catacctgct
      781 aacttcatcc acaacctctt taaagttatc taagaaaaat gattggtgct catggtaaaa
      841 aacaggtgga atagcattat caaagtggtc ggttaatgtc atatatccat atggagagtc
      901 atgatcaatg atatacgcat tagcaccaaa atccacagta tgatgtgttt tatttagtgg
      961 gagtgtttct tgatgattgg aaatttcaac cccaaaaaaa acaaaatcgt ggttagctat
     1021 ttcttcaata tcctcagaga atgtgttttt atatgtttgt atattctcag acttaatttt
     1081 atcaaaggac ttaatattaa gagtattatt tgacaggtta gcgttacttt gatgtctgaa
     1141 gttaaaatca gaattaataa taatagagag caagtgacgt tcatccgaag aaagaggatg
     1201 agttttttgt attatttcag aaagatagtg tctggacacc ttatcgaggt gctcaagtga
     1261 ccgaaatgat gaccgtgctc cagatggcgc ttcaatagaa agatttaatg atttgaaact
     1321 ggtcccttga gagaacattt tactataact ttgcgctgct atagacttcc ttaaaaagct
     1381 tttcacagta tcagcacagt gatctaaatg tttttcactt atgccagaag aggatgagtt
     1441 tgttacgcat gatatgtttt tttgtaatgc ttttgtatct ttgttcggat ttatttttcc
     1501 atcaacaaga tcaatattgt tctgaacatt aatatgattt actgcttcag gtattttcat
     1561 aaaacatccc ccattatgtg cgtttttatc tgcagttatt tagaataata ttatctaact
     1621 taatattttc ttgtatgtat tataacgaac atattttaat atgttgtacg attttcactc
     1681 tgaaatcccc tgagtttaat cacacttcct ttactgaaag gaattattgc aacagtatta
     1741 tttgtttttg gatatatatc ttgcgccttg ttatgtagtt aatgctcaga atatgataga
     1801 taaaatagag acgcaaaaca gggcatgctc ataacatgat gataacataa aaaattagta
     1861 acaataaatg aaagaatcat gggcgctcaa caaaaa
//
LOCUS       DADLTI010000321         1881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLTI010000321 DADLTI010000000
VERSION     DADLTI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.326"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN755_004286"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN755_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7382827.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="JN755_004287"
     CDS             complement(984..1814)
                     /locus_tag="JN755_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7382828.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          459 a          412 c          417 g          593 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc c
//
LOCUS       DADLTI010000322         1879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLTI010000322 DADLTI010000000
VERSION     DADLTI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.327"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1480
                     /locus_tag="JN755_004288"
     CDS             <1..1480
                     /locus_tag="JN755_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7382829.1"
                     /translation="FAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1488..>1879
                     /locus_tag="JN755_004289"
     CDS             1488..>1879
                     /locus_tag="JN755_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382830.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          521 a          368 c          458 g          532 t
ORIGIN      
        1 gtttgcgttc attattatcg tgttgctggt ggtatatttc atgaccactc gtgtgttccg
       61 tcggcaattt tctgaaatga ccgagctggt taatacgctg gcgtttttgc ccgactcaac
      121 ggatcagatc gaggctctga aaattcgcga aggcgatgcg aaagagatta tcagcatcaa
      181 aaattcgatc gcggaaatga aagatgccga aattgaacgg tcaaataaat tgctctcact
      241 gatctcttac gatcaggaaa gcggttttat taaaaatatg gcgattattg agtccaacaa
      301 taatcagtat ctggctgtgg ggatcatcaa actgtgtggt ctggaagccg tggaagcggt
      361 gtttggtgtt gatgaacgca ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa
      421 atatgcgcaa tgctgcgata tcgtgacatt caatgccgat ctctatttac ttctgtgtcg
      481 ggaaaatgta cagacattta cccgtaaaat agcgatggta aacgattttg acagcagctt
      541 tggctaccgc aatctgcgca tccataagtc tgccatttgt gaacctttgc agggggaaaa
      601 cgcctggagt tacgcagaaa aactgaaact ggcgatttcc agtatccgtg accatatgtt
      661 ctccgagttt attttctgtg atgacgcgaa actcaacgaa atagaagaga atatctggat
      721 tgcgcgtaat attcgccatg caatggaaat tggcgaacta ttcctcgtct atcaaccgat
      781 cgttgatatt aacacccgcg ccattctgag cgcggaggcg ttgtgccgtt gggtttctgc
      841 ggagcggggg atcatttcac cgctgaagtt cattaccatt gctgaagata tcgggtttat
      901 caatgagctg ggttatcaga ttattaaaac ggcaatgggt gaattcagac attttagtca
      961 acgtgcgtcg ctgaaggatg atttcttact gcatattaat gtttcgccct ggcagttaaa
     1021 cgaaccgcac tttcatgagc gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa
     1081 cagcctctgt gttgagatca ctgaaaccgt gatcgagcga attaatgaac atttttatct
     1141 caatattgaa caactgcgta aacaaggggt acggatatcg attgatgact ttggcaccgg
     1201 tttgtcaaac ctgaaacgtt tttatgaaat taatccagac agcataaagg tggactcgca
     1261 atttaccggc gatattttcg gtactgcggg aaaaattgtg cgcattattt tcgatctggc
     1321 acgctataac cggatcccgg tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga
     1381 attaattaaa ttaggatgcg ttcaggctca ggggtatctg taccagaaac ccatgccatt
     1441 ctccgcctgg gataaaagtg gaaaattagt aaaagagtag tttacgtatg tccagaatca
     1501 ataagttcgt acttacagtc agtctgctga tttttatcat gatttcagca gttgcctgcg
     1561 ggatctacac tcaaatggta aaggaacggg tgtatagcct gaaacagtcc gttattgata
     1621 ctgcttttgc ggtggcaaat attgctgaat atcggcgtag cgtggcaatt gatcttatca
     1681 acacgctaaa tcccacggag gaacagctgt tggttggttt gcgcacagct tacgccgact
     1741 cggtttcccc ctcttatttg tacgatgtcg gtccttatct gatttccagt gacgaatgta
     1801 ttcaggtaaa ggagttcgag aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc
     1861 gacatgtcaa aaatacagg
//
LOCUS       DADLTI010000323         1877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLTI010000323 DADLTI010000000
VERSION     DADLTI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.328"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..557)
                     /locus_tag="JN755_004290"
     CDS             complement(<1..557)
                     /locus_tag="JN755_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7382831.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JN755_004291"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JN755_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY7382832.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1544)
                     /locus_tag="JN755_004292"
                     /pseudo
     CDS             complement(1321..1544)
                     /locus_tag="JN755_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          536 a          319 c          396 g          626 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agcgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccaggctt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tggtgaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 cgaaatagaa ttaccgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagc
//
LOCUS       DADLTI010000324         1872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLTI010000324 DADLTI010000000
VERSION     DADLTI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.329"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..161
                     /locus_tag="JN755_004293"
     CDS             <1..161
                     /locus_tag="JN755_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7382833.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JN755_004294"
     CDS             655..1233
                     /locus_tag="JN755_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382834.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JN755_004295"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JN755_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1872
                     /locus_tag="JN755_004296"
                     /pseudo
     CDS             1584..1872
                     /locus_tag="JN755_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          607 a          335 c          444 g          486 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctacggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagctcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttccta ttgaaagtcg tcgtcagaca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag ca
//
LOCUS       DADLTI010000325         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLTI010000325 DADLTI010000000
VERSION     DADLTI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.330"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JN755_004297"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JN755_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          357 a          485 c          504 g          523 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttttggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADLTI010000326         1856 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLTI010000326 DADLTI010000000
VERSION     DADLTI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.331"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(172..450)
                     /locus_tag="JN755_004298"
     CDS             complement(172..450)
                     /locus_tag="JN755_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382835.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            391..576
                     /locus_tag="JN755_004299"
     CDS             391..576
                     /locus_tag="JN755_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382836.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(618..830)
                     /locus_tag="JN755_004300"
     CDS             complement(618..830)
                     /locus_tag="JN755_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7382837.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1033..1251)
                     /locus_tag="JN755_004301"
     CDS             complement(1033..1251)
                     /locus_tag="JN755_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382838.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(1425..1760)
                     /locus_tag="JN755_004302"
     CDS             complement(1425..1760)
                     /locus_tag="JN755_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382839.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          455 a          475 c          414 g          512 t
ORIGIN      
        1 agcggctggc gaacggcagg aacgaccccg gacggcagat tacgcatgct tttcggttcc
       61 ggctccacca gtacccgggt attgtggaat accggcatgg attcacggcc tggcttaagg
      121 accaccagcc cgagttccgg aaccagaaca ggtcgaagta atacccgcac attacctcca
      181 gatccgttgc tggaatgtgc aggacggacg cggtggccgt tcggaataag ggagcctgac
      241 gaagattatc cagtgacgat aatcgagact gagggctttc ttaatctcgt atccgtgtct
      301 gcggtagcgc tgaatcagcc attcagcctg ttcttcggtg caggagtcgt gctgatacca
      361 gtcagatttg aatgcatgag aacgccgccc gtgcctgctg gcaggggcgg cagagttatc
      421 cgaattgtaa aatttggtat cgtgcgccat ctgttttctc tgctggcgca gcaggtgcca
      481 gttgttcagg ctgacggatg gattgtaaac cagaacgacc agaaaaaaca aaacccgccg
      541 aagcgggtta agtgcgggtg cgttgaggat gcctgacaca tcagcggtgg cgagggattt
      601 ctcccccgcc gggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc
      661 tggccggttc ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg
      721 acggttaaac agatgacgat aagggcgatt aacatcgcct tttgctgctt catagcctgc
      781 ttctccttgc ctttcggcac gtaagaggct aacctacatg tgttcagcat ggattgagcc
      841 tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta actggggctt ttctctatct
      901 gccttttggt gttcatgcct gagacagata gcctcaagca cccgcagtca ttctacttaa
      961 ctaagatttc cctgcaaacc gtttttgtcc ggcacagtaa atatccaact aaaccaatag
     1021 cgttcgctgt atttaccgcc agtattcaat gcacatgacc gccatgaaca cccctaaaaa
     1081 aagggcattt atatgtccaa acattaatat caaaacatca attttttcca tataccttgc
     1141 tgtgaagatg atgggcatac atgatgcgaa caaccagaac gcaacaaaca aaaactgcaa
     1201 tgcgtttttc attattcccc ctacaatcaa tgtgcaataa catttaaaca cacctcaatt
     1261 tggccggaca tataaatatc taaaccagaa aaaatcactt acatagcgtt acaaactctt
     1321 tagtctaaat actcatcgta aaacattccc catacttatc agtccgttcc gcgccaggta
     1381 gcttattgcc ttatctggca acctataatc aggtttccgc tttttcagtt ggctggtcgt
     1441 ttaaccgaca tagttaaccc attaatctgg ttgccggatg ctggtggatt ttcgcgtttt
     1501 agttgttcat aaaagtgcac agctttaacc agttcttctg atgtaaccgg gactggtggg
     1561 gcagtgaata aggcctgaat ttcatagttc ggcctgtcgt tacaatcctc ttttttcggt
     1621 acatatttcc agtcaccaac ccacaactcc ccctgagagt ccataacacc ttttttcacg
     1681 tagcgatatc gccacgctat cggctctgct tccagcgatg cccgtgcaat tttgaataac
     1741 tcgccctcta ctcgcgccat ccctgaattg gggtggcatt tcgtaatcgc tatttttaat
     1801 ttggcttctt cgattaattg ttcttttgtt aattcagtca ttttcattac cgccct
//
LOCUS       DADLTI010000327         1852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLTI010000327 DADLTI010000000
VERSION     DADLTI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.332"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..559
                     /locus_tag="JN755_004303"
     CDS             <1..559
                     /locus_tag="JN755_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7382840.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            998..1835
                     /locus_tag="JN755_004304"
                     /pseudo
     CDS             998..1835
                     /locus_tag="JN755_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          454 a          454 c          565 g          379 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatcacggag ttccagctcc ctgcaggtaa
      121 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      181 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      241 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      301 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      361 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      421 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      481 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      541 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      601 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      661 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      721 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
      781 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
      841 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
      901 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
      961 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1021 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1081 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1141 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1201 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1261 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1321 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1381 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1441 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1501 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1561 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1621 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1681 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1741 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     1801 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DADLTI010000328         1846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLTI010000328 DADLTI010000000
VERSION     DADLTI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.333"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..356)
                     /locus_tag="JN755_004305"
     CDS             complement(<1..356)
                     /locus_tag="JN755_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7382841.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGCP"
     gene            complement(356..1276)
                     /locus_tag="JN755_004306"
     CDS             complement(356..1276)
                     /locus_tag="JN755_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7382842.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1414..>1846
                     /locus_tag="JN755_004307"
     CDS             1414..>1846
                     /locus_tag="JN755_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7382843.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAG"
BASE COUNT          460 a          454 c          427 g          505 t
ORIGIN      
        1 gggcaccccc cacacacacc cagcacgcca atgcgttcag catcaacata tggcagagtt
       61 actgcataat cgataacacg gctaatatcc tctacgcgct gtgtcggatc ttctatccaa
      121 cgaggctccc caccagattc tccctggaaa ctggcgtcat aagcgagaac gacatagcct
      181 ttttcagcca atgccttgcc atagatattg ccagacgtct gctccttaca actgccaaaa
      241 ggatggacgc tgataatcgc cggatactgt tttgactcat caaaatttgg tggaaactga
      301 atattggcag caatatgcca ggccatatca ctattgcgga tttgaatatg tttcattaca
      361 gagtttcctc aaagaacgga gctaatactg aaattgcttc agccacatag gctttgccgt
      421 cataaagatc catatggttt gcgccttcaa caatgtgata gcgtttatcc tggcttgatg
      481 cccgatcgta caggtcgtca ctcatccatt tgctccctgc ctggctgccc gccacaatct
      541 gcatcggctg agtcaggtac acttccgcca tatggtaagc atcataggta ataatctggt
      601 taaggctgcg caaagtagcg taacccggtg ctgttggata ctgcgcacga ggtgtgtggt
      661 aatactccca ggcctgacgc agttcttcgt tcggcgcatc ggcctccttc attggagcca
      721 gtggcataat ggcgtattct tcgctgctaa tatcgctggt tcgggcgttt gagccagctt
      781 caatgtgtgg cagagcatca atagatttca cattgttttc ccaaccattg cggaacatcg
      841 aaccaatatt gaccgcacta acggtaccga tggccttgat gcggcgatcc tgaattgcag
      901 cattggctgt atatcctgca ccggcacaaa ttcccatcgc accaattcgg gtattgtcga
      961 cataagaaag cgttgtcagg taatcaatca cggcactgac gtcttcagta cgaatgtatg
     1021 ggttttctaa ctgacgcggc tcgccgccac tttcaccttg ataagatgcg tcataagcaa
     1081 tagtgacaaa tcccttttcc gccagctttt cggcataggt tccggccgtt tgctctttaa
     1141 cgcccccacc tgggtgagat accacaattg ccggatactg acgggtttca tcaaattttg
     1201 gagggaaata gatcactgca gacaaagaga tggttggatt attgctgtta gtgaagctga
     1261 ctttattatt catcattcag ttcctcatgg agttgtcggt tcgttttgac ggttggtgat
     1321 atcactatag atattgatca ttaagttgat tagaccctaa atcatgatta gacctatcat
     1381 ttaaatgatt aatagattgt atggagatac tgaatgaagc gtgaagaaat cgctgatctg
     1441 atggcgtttg tcgtcgttgc agaggagcgt agcttcactc gcgcagcagc ccgcctgagc
     1501 atggtgcagt cagctttaag ccagatagtg cgccgtatag aagaacgatt gggattgcgg
     1561 cttctgacgc gaaccacgcg cagcgttgta cccactgaag cgggcgagca tcttttgtct
     1621 gttcttggcc ctatattgca tgacatagat tctgccctga catccctgag cgatctgcag
     1681 aaccgcccat ccgggacaat acgtattact actgtagaac atgcagcaaa aacgatattg
     1741 ttaccagcca tgcgcacatt cctgaaatcg catcctgaaa ttgatattca gcttaccatt
     1801 gattatggtt tgaccgatgt cgtttctgaa cgttttgatg caggcg
//
LOCUS       DADLTI010000329         1841 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLTI010000329 DADLTI010000000
VERSION     DADLTI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.334"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            16..>1841
                     /locus_tag="JN755_004308"
     CDS             16..>1841
                     /locus_tag="JN755_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7382844.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNIS"
BASE COUNT          533 a          436 c          474 g          398 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cgtctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta a
//
LOCUS       DADLTI010000330         1834 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLTI010000330 DADLTI010000000
VERSION     DADLTI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.335"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..222
                     /locus_tag="JN755_004309"
     CDS             <1..222
                     /locus_tag="JN755_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382845.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(864..1136)
                     /locus_tag="JN755_004310"
     CDS             complement(864..1136)
                     /locus_tag="JN755_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382846.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1225..1404
                     /locus_tag="JN755_004311"
     CDS             1225..1404
                     /locus_tag="JN755_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382847.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1456..1650
                     /locus_tag="JN755_004312"
     CDS             1456..1650
                     /locus_tag="JN755_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382848.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          582 a          422 c          360 g          470 t
ORIGIN      
        1 atagccaatt ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt
       61 acaggtttta ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc
      121 gacaacggtg ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg
      181 tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
      241 tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt
      301 tgtataaaat aaaaaattat cgtaagtaca tctatctgcg atgtcttgtg aaaattatcc
      361 tgtctggcaa caatcgcgtc catctgtatt aatggacgcg aacgatgaac tctcaaacaa
      421 cactagatat caccggcttt cgcacttatt tgccagaatc attacgtctc agattcgacg
      481 agcaattatc tgtccgggct atcgcacagc agttaagact cagttattcc cccattcaca
      541 atctctttca acgaattaac gcctcaggta ttgtctgacc atgacccgag tccctttcat
      601 tcgcccaacc gataccatac tttatgccaa cagaaagaat gaatgaaccg cgcctaaaat
      661 cagcaaggac acatggcaca aagtgcgaca tgtctcacat ttaaccccag tgggggcaac
      721 aagtataaaa ccgttttttg attaagtggt cgaatatgaa aaggggaagg tttatgtgaa
      781 aaatacctgt gttttctttg aaaacacaca acagtatcag gttatttatg tttatacagt
      841 gcgttccaaa ctgacctgaa taattacgca agttgcaaca ataaaacatt tttgccgtag
      901 tgtgaggtac tccggtaaat tgctttccag gtgcagacaa tgactaacga agaactacag
      961 gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag tacaccagca acgagagtct
     1021 ttgcttaacc catctccatt caccaggcca aaacgattgg agcctgaagc attcttggac
     1081 aaatacccac ggcggcttaa atcaggcaaa accagaccac caggctgata gctcacagtt
     1141 tgttctccga ttcaataaaa cggataagaa ccatcagcgc ggtagcgtaa ggcactacag
     1201 cgcattgctt tacgagtaat aacaatggat ttatcaccag aagacgcaaa gtttttacga
     1261 gaactgcaac accgtatgct cagagaagcc attatcgaac agcaaacggg cataagcgcc
     1321 atagacccat tgcgatacaa acgggcctca tctcctttag ttgacgacaa ttccaatgcg
     1381 gataaaacac gtccaccaca ctgattacat ttttcattag cacagtggtg tgatgcagca
     1441 tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc tggagcaact gttacaggca
     1501 cgccaacggg agtttgccac taaatgggga cgtgccgcgc tgccacatga acgcctaacc
     1561 tattttccac ttgaagacct cattaaaaat aatggcaaaa aaatcaggtc tgataagaaa
     1621 cacttcttag aatgcaatga agacaaataa caacaatcta catattgcca ccaaccacag
     1681 atgtggcatc gttatcagcg cacttgattt tctttatatc cactgacgag cttgccgggg
     1741 cacctttgtt tgccggtcat tctggtatca tgcagatatt tacggcgtac aagcccacta
     1801 gcatgacatt gtaataaggc ctgagcctaa cgct
//
LOCUS       DADLTI010000331         1782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLTI010000331 DADLTI010000000
VERSION     DADLTI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.337"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..452
                     /locus_tag="JN755_004313"
     CDS             <1..452
                     /locus_tag="JN755_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382849.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            455..988
                     /locus_tag="JN755_004314"
     CDS             455..988
                     /locus_tag="JN755_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7382850.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JN755_004315"
     CDS             complement(1017..1544)
                     /locus_tag="JN755_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7382851.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JN755_004316"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JN755_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          498 a          392 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcagag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADLTI010000332         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLTI010000332 DADLTI010000000
VERSION     DADLTI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.338"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..284
                     /locus_tag="JN755_004317"
     CDS             <1..284
                     /locus_tag="JN755_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382852.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JN755_004318"
                     /pseudo
     CDS             286..890
                     /locus_tag="JN755_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JN755_004319"
     CDS             877..>1773
                     /locus_tag="JN755_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382853.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          425 a          403 c          496 g          449 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggcaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 tcagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADLTI010000333         1758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLTI010000333 DADLTI010000000
VERSION     DADLTI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.339"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..262)
                     /locus_tag="JN755_004320"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="JN755_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(377..1015)
                     /locus_tag="JN755_004321"
     CDS             complement(377..1015)
                     /locus_tag="JN755_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382854.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1174..1644)
                     /locus_tag="JN755_004322"
                     /pseudo
     CDS             complement(1174..1644)
                     /locus_tag="JN755_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          499 a          434 c          318 g          507 t
ORIGIN      
        1 gcaagcgctg taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga
       61 catcctctcc ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa
      121 gcaacaggct gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct
      181 ccattgtctg ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca
      241 gccccagcaa agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc
      301 tatctcttat tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat
      361 gtacaggctc tcagcatcag caaagcctct tacttttcca actactactg ttagcatagt
      421 gatcaaattc ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg
      481 catctggcga tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca
      541 aaaacataat tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag
      601 aaatcattac tacacttcct tttgaagtat tttgtaaaat attttctgca atacgctcac
      661 gaaatttact ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta
      721 aatgcctatg aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc
      781 ttactccata ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat
      841 ccccaccaga cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca
      901 tatcaccaag acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct
      961 cctctgtatg tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt
     1021 atcctgacag tatatatact cactatattt tcttcataac aaatatccct ctgatatgac
     1081 aacaaaaaac ggagccggat tcctgttttt gtgaactgca tctattcgtg tctaaaaagt
     1141 gatccactta ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc
     1201 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     1261 gttgtacgca taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgct
     1321 catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat gttatcctcc
     1381 ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca gtgtcctggc
     1441 ggtatcacgg ccccctgcgc cgttatgtgc catttcaacg atctgctcct taacgccggg
     1501 ttttctggct tcataagagt aagtgagctg gaaaacacgt ttacatgagc gacaacggaa
     1561 acgctcatgc cgggaacaac tacggccatg tcgataaact tcatcggaat gacagcgcgg
     1621 acaatgaaca gtaacggtag ccatggaaaa ccttaaatac cagtagatta ccagaaactt
     1681 ctgtcgattg aagacatcac ccgaagtttg aattcattcg aatacttttt acgaggttct
     1741 tttcgccagt cctgtaat
//
LOCUS       DADLTI010000334         1712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLTI010000334 DADLTI010000000
VERSION     DADLTI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.340"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>1712
                     /locus_tag="JN755_004323"
     CDS             <1..>1712
                     /locus_tag="JN755_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7382855.1"
                     /translation="LEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVF
                     VSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHI
                     SPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFR
                     DPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLA
                     YRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQL
                     SLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGYVALNLPAADKPSRYLLTVSASD
                     GAAYRVTTTKELLIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRL
                     EDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTV
                     DIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDT
                     LYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGEL
                     VNVE"
BASE COUNT          399 a          446 c          469 g          398 t
ORIGIN      
        1 ctggaaggcg catccagcaa tttcatctcg ccgcagccgg gtaatattta tattcctctc
       61 ggccaacaag agccggggct gtacctcgtc gaggcgatgg taggtgggta tcgggcgacg
      121 acggtggtgt ttgtttccga taccgtggcg cttagcaaag tgtcaggtaa cgagcttctg
      181 gtatggaccg cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat cttgtggact
      241 gacggtcttg gcgtgatgac ccgcggggtg accgatgaca gcggtacttt gcagttacaa
      301 catatatcgc cagaacgttc atacattctg ggtaaggatg ctgaaggcgg cgtttttgtc
      361 tccgagaact tcttctacga aagcgaaatc tacaacaccc gcttgtatat ttttaccgat
      421 cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg cgagttccgc
      481 gatccgttgc attcatcccc catcgtcagc gccccggcga agctttcggt gctggacgct
      541 aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg cgggcaggga
      601 agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg agttacgtct tgcttaccgc
      661 aatcaggtct atagcagcag ttttcgcgtg gcaaactaca tcaagccaca tttcgagatt
      721 ggtttagctc tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg caaactgcaa
      781 ctgctctacc cggatggcga gccggtaaaa aatgcccgcg tgcagttaag tttgcgcgct
      841 cagcaattat caatggtcgg taacgatttg cgttatgccg gacgtttccc cgtgtcgctg
      901 gaaggcagcg aaacggtgtc cgacgccagc ggttatgtgg cgttaaatct ccccgccgcc
      961 gataaaccga gccgctattt gttaaccgtc tccgccagtg acggcgcggc gtatcgcgtc
     1021 accaccacca aagagctcct cattgaacgc ggcctggcgc attactcttt aagtaccgcc
     1081 gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct ggaatcttca
     1141 aaacaggttc ctgttacgta tgaatggttg cgtctcgaag accgcacgag ccatagcgga
     1201 gagctaccgt caggcggcaa atcctttacc gtcaatttcg ctaaacctgg caactacaat
     1261 ctgacattac gcgataaaga cggcttaatt ctcgctgggt taagtcatgc cgtcagcggt
     1321 aagggcagca cggcgcatac tggtacggta gatatcgtgg cggataaaac gctgtaccag
     1381 ccaggcgaaa ccgcgaagat gctgattact ttcccggagc caattgatga agcgttattg
     1441 acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt cgcatccggc aaactggcta
     1501 acgctacaac gtttaaacga taccctgtat gaagcccggg ttccagtgag caattccttt
     1561 gcgcctaaca tcactttttc ggtgctgtat acccgtaatg gtcagtacag ttttcagaac
     1621 gccgggatca aagttgccgt tcctcagctt gatatccggg tgaaaacgga caaaacccat
     1681 taccagcctg gtgaactggt caatgtcgaa tg
//
LOCUS       DADLTI010000335         1698 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLTI010000335 DADLTI010000000
VERSION     DADLTI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.341"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(603..939)
                     /locus_tag="JN755_004324"
                     /pseudo
     CDS             complement(603..939)
                     /locus_tag="JN755_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          551 a          338 c          329 g          480 t
ORIGIN      
        1 aatgtgtgtc atacactcag tggcgtaatc gttgacaata tggtgcatgt aatcgtaagc
       61 gtccagcgaa atccatctgg tcataatctg aagcatccga caaagtggtg tccaccaaat
      121 aagtgatctg cgcccaatat cctgagccgg aactttgaca aaggccaacg aaaaatggga
      181 gcggatgtta cagagttctc ggtacagggg aaaagtggta cctgtcgcca gttctccgat
      241 ctgtttaacc gggaaattat ctcctacagc ttgtcagaaa ggtcggtgat ggagcatcgt
      301 taatacgatg cattcacagt gcccggccag aggatgcccc gtcgctgcat atggatcagg
      361 gttggcaata tcgaatcgca ggctataggc aaagttaaag ccccatggag tagcacaaaa
      421 tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg aagaacttct tcagcacact
      481 gctaaaacgc atagtgatcg agcgctgatt ctggcgaaca actgaactaa tacatcagaa
      541 tctgcattat gttaaataaa tatgaaaaga tggtttaaat accccgttac ttttgactta
      601 cactatacgg tatcgcatcg tttaatattc gcaccggcca gatttttatt tctattagtt
      661 gtcacaatac tgaatgcgta cgaccacagt attctggctc ctgtgtggtt atgctttaat
      721 tctgcgttcc gggcagataa gcagttgctt gcaggaatcc ttcttgtgtt aatgtcagtt
      781 ccccttttta ccagtgctga tttccacatt ccgtccaaca gagcttatag cctttccctg
      841 gattatagca ttgtccggct gaagttcttt ttgaataata atagaagcac tgctggcaga
      901 tccagtccgt ttttcataac ccactgtact gataaccata atctaatcag tagaaattga
      961 gtcgaaaata agcactactc catacaggat aattagaggt cagtttgatt attcacaatt
     1021 catcatcagc attttctatt tctgacgaaa tcaacatgaa aataaccata tatgataatt
     1081 attataataa cggctttaat tggaatacat atattacaac gtattatata taattggtat
     1141 tctgggaact atattctcaa aatacagtag aaacgaggta tgtttctggt ggaaaggaca
     1201 gtgggattaa aaagtaagaa ttgataaaaa aaacgccagc aacacatagt gccggcggag
     1261 ggaatacccc atggagaaaa tgtgatgcct agagcatcat gaatatataa ttaaaaatag
     1321 ttagcgttgt caatactttc acaaaaataa ttttcgtagt atagaaagat atttttatgc
     1381 atgacctacc tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac
     1441 acaatattca tagtatggcg atgcctacgc cagcaagaat agcgccaata atattgggaa
     1501 tataatagac accagacgca caggcatctc actccttaac aaacaataat caggatattt
     1561 acttttaccc agctaactgt ttacacccaa agtgcacaca attaaccatt caataacaaa
     1621 tggcaatatc catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat
     1681 tctgggaaat ctatcatt
//
LOCUS       DADLTI010000336         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLTI010000336 DADLTI010000000
VERSION     DADLTI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.342"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(72..539)
                     /locus_tag="JN755_004325"
     CDS             complement(72..539)
                     /locus_tag="JN755_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7382856.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JN755_004326"
     CDS             complement(605..844)
                     /locus_tag="JN755_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY7382857.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JN755_004327"
     CDS             complement(979..1584)
                     /locus_tag="JN755_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7382858.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          542 a          370 c          293 g          474 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgct cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADLTI010000337         1654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLTI010000337 DADLTI010000000
VERSION     DADLTI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.343"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(155..1390)
                     /locus_tag="JN755_004328"
     CDS             complement(155..1390)
                     /locus_tag="JN755_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7382859.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="JN755_004329"
     tRNA            complement(1537..1613)
                     /locus_tag="JN755_004329"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
BASE COUNT          420 a          452 c          393 g          389 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtc
//
LOCUS       DADLTI010000338         1642 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLTI010000338 DADLTI010000000
VERSION     DADLTI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.344"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..132
                     /locus_tag="JN755_004330"
     CDS             <1..132
                     /locus_tag="JN755_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAY7382860.1"
                     /translation="ASANNIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            132..302
                     /locus_tag="JN755_004331"
     CDS             132..302
                     /locus_tag="JN755_004331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382861.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            377..652
                     /locus_tag="JN755_004332"
     CDS             377..652
                     /locus_tag="JN755_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382862.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            752..>1642
                     /locus_tag="JN755_004333"
     CDS             752..>1642
                     /locus_tag="JN755_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7382863.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLT
                     RDVATGVLARSMDVDIYNLHP"
BASE COUNT          519 a          368 c          369 g          386 t
ORIGIN      
        1 gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca
       61 tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg
      121 gagtcatggt aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg
      181 aagaaagcaa aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg
      241 aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt
      301 aattaactga ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt
      361 aattaaggag aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat
      421 tgttcacgag gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa
      481 agaaacccca ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa
      541 cgatggtgta aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa
      601 agccgaattt ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga
      661 aagcctgcac actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag
      721 taattttaat ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact
      781 gtattacgaa gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta
      841 ctgggctgaa gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga
      901 tgcaggctac gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga
      961 tgacctcccg ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg
     1021 ggaagatggc atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc
     1081 cagcactgac aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct
     1141 cctgccagtt tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa
     1201 accggtaacg cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga
     1261 actaccagct gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga
     1321 aattcgcgat ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa
     1381 ttcaagcctg gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga
     1441 tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac
     1501 ggcttccggc gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgtcca
     1561 cgatctgacg tcactgacgc gcgatgtagc cactggcgta ctggcccgtt caatggacgt
     1621 ggacatctat aaccttcatc cg
//
LOCUS       DADLTI010000339         1619 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLTI010000339 DADLTI010000000
VERSION     DADLTI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.345"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(821..1060)
                     /locus_tag="JN755_004334"
     CDS             complement(821..1060)
                     /locus_tag="JN755_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7382864.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1619)
                     /locus_tag="JN755_004335"
                     /pseudo
     CDS             complement(1255..1619)
                     /locus_tag="JN755_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          363 a          450 c          386 g          420 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttg
//
LOCUS       DADLTI010000340         1578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLTI010000340 DADLTI010000000
VERSION     DADLTI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.346"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..175)
                     /locus_tag="JN755_004336"
     CDS             complement(<1..175)
                     /locus_tag="JN755_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7382865.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            174..1313
                     /gene="queG"
                     /locus_tag="JN755_004337"
     CDS             174..1313
                     /gene="queG"
                     /locus_tag="JN755_004337"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7382866.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1296..1349)
                     /locus_tag="JN755_004338"
     CDS             complement(1296..1349)
                     /locus_tag="JN755_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382867.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          407 a          390 c          406 g          375 t
ORIGIN      
        1 caggatacgc cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat
       61 agagtgtgag ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt
      121 accagacggt gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag
      181 agcccctcga tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct
      241 ttcagcaggt aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat
      301 ggctggacaa acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg
      361 ctcgccccca tgagttatta cccggtacgc tgcgtgtaat cagcgtgcgg atgaattacc
      421 ttcctgctaa cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc
      481 gttatgcgct gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg
      541 agatgattca gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta
      601 ttctcgagcg cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta
      661 tcctcaatcg cgaggccggt tcgttcttct ttttaggcga attgctggtc gatattccgc
      721 tgcccgtgga tcaaccagtc gaggaaggat gcggcaaatg cgtggcctgt atgacgattt
      781 gcccgaccgg tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc
      841 tcaccatcga acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta
      901 tttacggttg cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta
      961 cagaagacga tttcagcccg cgtaagccgc tacacgcacc ggaactcatt gagttatttg
     1021 cctggagcga agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc
     1081 acctgcgctg gctgcgtaat atcgccgtgg cattaggcaa tgccccttgg gatgaaacga
     1141 ttttgacagc gctggaaagt cgtaaaggtg agcacccact tctcgatgag cacatagcgt
     1201 gggcgattgc gcagcaaata gagagacgaa atgcgtgcat agtcgaagtg caattgccga
     1261 aaaaacagcg tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc
     1321 acagcttgtg aataaaaata aaaacgcatt gcaattcaag acgaataaaa tcgtcaagtg
     1381 atcgactcga caaaacggaa tcttgatttt taacttattt atcaataggt taaaaaatac
     1441 cattcaccgt gcgaagtttc tttgaaatta agattacagc ctgaggctgt ggataactct
     1501 gtttacaaga gtttgtcaga gacaacaaaa accagcaata acgcatcgcg tcgctgtgga
     1561 tattttattg agagaaga
//
LOCUS       DADLTI010000341         1512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLTI010000341 DADLTI010000000
VERSION     DADLTI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.347"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            425..1498
                     /locus_tag="JN755_004339"
     CDS             425..1498
                     /locus_tag="JN755_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR"
                     /protein_id="HAY7382868.1"
                     /translation="MHTRTKLPAPLAADVAALIKNGMDFLDKAREEFEAKQYKHSVVS
                     FWIAVEILLKVPLASEHWTLVCSGKKVSRKSYLAGDFQSVSFDDVCTRLRDILEKPLP
                     KETEAVFNTIRNHRNRVVHFFHTAFSDSEVETILAEQARAWFALNRLMREDWQQHFAS
                     PHNWALALGETQLLRGNEFYAEARLKHIQPELEQLATEGAEFHPCTICHKPAAIMEIL
                     AVGKNGPTVYEQTCRVCFHSERHVKFTCPECDTDQVLPVEEEDDDTFICRTCNAELSR
                     YNLLDEENFRHVDEMMYPDGLANCAHCEGHETVCVFGENFLCTRCLEIHTGYDTCEIC
                     GTPCEAMGETMRYTGCPHCADED"
BASE COUNT          386 a          354 c          385 g          387 t
ORIGIN      
        1 ccgctttgtg ccaggagcgg acgttaaggg tttccaaggg agctatctaa tatggcatcc
       61 acggtgaacg cattgccagc cgggaaatga tgcgaataac tgtgtttaat tttatcgagt
      121 gagattacaa tcggtggcga aatcactgtg ctgtggcgga ctcagctcgg aatttattga
      181 aactataacc tcgctagggt cttgctcaca ttgggtagga tcagtcttgt aatgcacctg
      241 ttgtatggcc ttgttcatcc gaagatcctg caatggctga tggtgcatga gaagtttgag
      301 tgcaatgagg tcaacaccaa agtctttgcc ctcgatgtgg tggccagcca gcaactaaga
      361 tttatcctct atacccacta atctctgcag cagctgccgg ccccccgtga taacggagat
      421 caggatgcat acacgaacaa aattacctgc ccctctcgca gctgatgtgg cggctctcat
      481 taagaatggc atggattttc tggataaggc acgtgaagag ttcgaggcca aacagtacaa
      541 acactcggta gttagtttct ggatagctgt agaaattttg ctcaaggtac cccttgccag
      601 cgaacactgg acgctggtct gcagcggtaa aaaagtatcg cgtaaaagct accttgccgg
      661 tgattttcag tcggtgtcct ttgatgatgt ctgtacccgg ttgagggata tccttgaaaa
      721 gcccctgcct aaagaaaccg aagctgtgtt taataccatt cgcaatcacc gaaaccgtgt
      781 agttcatttc tttcatacag cttttagtga ttctgaggtg gagaccattc tggctgaaca
      841 ggctcgggca tggtttgcac tgaaccgact aatgcgtgaa gactggcaac agcattttgc
      901 ttcaccacac aactgggcac tggcattagg tgaaacacaa ctcctgcgag gcaacgagtt
      961 ttatgccgaa gcccgtctga aacatatcca gcccgagctg gaacaacttg cgactgaggg
     1021 cgctgaattt catccctgca ccatttgcca caagccagca gccatcatgg aaattcttgc
     1081 cgtcgggaag aacggtccta ccgtttatga gcaaacttgt cgcgtctgtt ttcactcaga
     1141 gcgccatgta aagtttacct gtcctgagtg tgatacagac caggttctgc cggtggaaga
     1201 agaagatgac gataccttca tctgccgcac ctgtaacgca gaactctcaa ggtataactt
     1261 gcttgatgag gagaattttc gtcatgtgga cgaaatgatg tatcccgatg gcctagcaaa
     1321 ttgcgctcac tgtgagggcc atgaaacggt ctgtgtgttt ggcgagaact tcctgtgcac
     1381 acgatgtctt gagatccata caggctatga tacttgtgaa atttgtggga ctccctgtga
     1441 agcaatgggc gagacaatga ggtatacggg ctgcccacat tgtgcggatg aagactgaat
     1501 cgccacaaat tc
//
LOCUS       DADLTI010000342         1495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLTI010000342 DADLTI010000000
VERSION     DADLTI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.348"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..1071)
                     /locus_tag="JN755_004340"
     CDS             complement(<1..1071)
                     /locus_tag="JN755_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7382869.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          447 a          239 c          321 g          488 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag cgtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatg
//
LOCUS       DADLTI010000343         1489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLTI010000343 DADLTI010000000
VERSION     DADLTI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.349"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(195..347)
                     /locus_tag="JN755_004341"
     CDS             complement(195..347)
                     /locus_tag="JN755_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382870.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            365..556
                     /locus_tag="JN755_004342"
     CDS             365..556
                     /locus_tag="JN755_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7382871.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            867..1385
                     /locus_tag="JN755_004343"
     CDS             867..1385
                     /locus_tag="JN755_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7382872.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT          432 a          326 c          281 g          450 t
ORIGIN      
        1 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
       61 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
      121 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
      181 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
      241 tccataacat taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt
      301 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
      361 ttttgtgagt caggcaacta gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
      421 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
      481 tgcctggctg caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
      541 accggtacaa cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg
      601 tcggatgctt ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt
      661 acgtctttgt cctgactgat gtttatcctg tttggctgcg aaataaatat aaaattaata
      721 tatatgttgt aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa
      781 tctatccctt tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt
      841 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
      901 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
      961 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
     1021 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
     1081 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
     1141 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
     1201 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca
     1261 aagtgtatac ctccacccag gagggtaaag agtgccagtt tacgacaggt ttagccgttg
     1321 ttattaccac aacgtataac gaaacgcgta ttcagccaaa taccaaatgt cctgaaaaga
     1381 gctaataatc aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa
     1441 gctatccggc tatagcctca tcattgcagg atcaactctc tgctgtcgc
//
LOCUS       DADLTI010000344         1478 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLTI010000344 DADLTI010000000
VERSION     DADLTI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.350"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            53..682
                     /locus_tag="JN755_004344"
     CDS             53..682
                     /locus_tag="JN755_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7382873.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(821..>1478)
                     /locus_tag="JN755_004345"
     CDS             complement(821..>1478)
                     /locus_tag="JN755_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7382874.1"
                     /translation="YYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILW
                     AGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGE
                     YKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMI
                     ALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
                     "
BASE COUNT          388 a          376 c          363 g          351 t
ORIGIN      
        1 tttcatgctt gctggtgaat agtcagtatt ttccctgact tgcgaactca ccatgacccg
       61 cacactcaag ccgttaattc ttaacaccgg cgcactggcg ctaacgttaa tcctgattta
      121 taccggcatt tcggcccatg acaaactcac ctggctgctg gaagtgacac cggtgattat
      181 cgtcgtgccg ctactacttg ccaccgccag acgttacccg ttaacgctgc tgctctatac
      241 gctcatcttc tttcacgcca tcatcctgat ggtaggcggg cagtacacct acgcgaaagt
      301 ccccgttggt tttgaggtgc aggaatggct ggggttgagt cgtaatccgt atgacaagct
      361 ggggcacttt ttccaggggc tggtgcctgc actggtggca cgagaaattc tcgttcgcgg
      421 gatgtacgtg cgcggacgta aaatggtggc gtttctggtc tgttgcgtgg cgctggcgat
      481 aagcgcgatg tatgaattaa tcgagtggtg ggcggcactg gcgatgggcc agggagcgga
      541 tgattttctc ggtacccagg gcgaccagtg ggacacgcaa tccgatatgt tctgcgcgct
      601 gcttggcgca ttaacgacag tgatattgct cgctcgcttt cattgccgcc agctacggcg
      661 ctatggcttg ataaccggat aacaacgtgc actttgttga tgccggatgc ggcgtgaatt
      721 ccttatccgg tctacaaaat ttgcaaatcc aaagcattac attaagacgt ggcctgataa
      781 acatatcgca tcaggccatt ttcgttagct attgtccacg tcaggctttc acatcactca
      841 ctgcaccgac cggaatatca gcatgaacag gctcgggcgg tactttgcga tatttatcca
      901 gcagatgaat ctggatcctg cgcagcgtgt cgccattgag gcgatagaaa cggaagtaga
      961 gaatcagcgt taccatgaaa aacagagtcg gtagagcaat cataataaac tgcataccta
     1021 atagggcttg cgtagactgt tcaacattcg gtacatagcc aatcatccct aacacaaccg
     1081 caatgaaaaa agccgcaaag gctgaaccgc ccttcaccac catagtctgc acggagtaag
     1141 cgatgctttc acagcgtacg tgcagtttat attcaccgta atcaacggta tctgccacca
     1201 tgatgacctg taatacccag aaaagcgccg ttcccacatt cagcaaaata cccgcaatca
     1261 caatgaggac gacgttgtga tagctcatta atgccattaa caggagaaca ccacagctta
     1321 acaccggaag aatagatgct ccggcccata aaatgcgtcg ggataatgat ttaactaagc
     1381 gtgggaagaa tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga
     1441 acaaatccgc atcaccgata acatatgaga aataatag
//
LOCUS       DADLTI010000345         1411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLTI010000345 DADLTI010000000
VERSION     DADLTI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.351"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..680)
                     /locus_tag="JN755_004346"
     CDS             complement(<1..680)
                     /locus_tag="JN755_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7382875.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLT"
     gene            777..>1411
                     /locus_tag="JN755_004347"
     CDS             777..>1411
                     /locus_tag="JN755_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7382876.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT          407 a          288 c          336 g          380 t
ORIGIN      
        1 ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
       61 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
      121 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
      181 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
      241 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
      301 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
      361 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
      421 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
      481 ggcggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
      541 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
      601 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
      661 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
      721 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
      781 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
      841 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
      901 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
      961 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     1021 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     1081 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     1141 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     1201 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagaagg
     1261 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa cggctagcag
     1321 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     1381 ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADLTI010000346         1397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLTI010000346 DADLTI010000000
VERSION     DADLTI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.352"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..240
                     /locus_tag="JN755_004348"
     CDS             <1..240
                     /locus_tag="JN755_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY7382877.1"
                     /translation="ALAGASAQGAAQALAASGVGSARQSASSAAGAVGDKASQNLNST
                     GSATASSASTASRATGDARSRSRAAVQRMQERYQR"
     gene            450..1136
                     /locus_tag="JN755_004349"
     CDS             450..1136
                     /locus_tag="JN755_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7382878.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1129..>1397
                     /locus_tag="JN755_004350"
     CDS             1129..>1397
                     /locus_tag="JN755_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7382879.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPV"
BASE COUNT          300 a          455 c          403 g          239 t
ORIGIN      
        1 gcgcttgccg gtgcatccgc acagggtgcc gctcaggcac ttgccgcgtc gggagtaggt
       61 tcagcccgtc aatccgcctc ctccgcagcc ggtgcggtgg gggataaagc ctcacagaac
      121 cttaacagca ccggcagcgc aacggcctcg tctgcctcca cggcctcccg cgccaccggc
      181 gatgcgcgca gccgcagccg cgccgccgtt cagcggatgc aggaacgcta ccagagataa
      241 cccagtctga ctgaactgcg ggacgccgca acggcgtcct gcggcttcgt cacgtcttta
      301 ccacaggaaa aacaccatga aatacggcat cacgctgctg atgctgtgcc tgcttgcagg
      361 ctgcgcacag cgaaacaccc aacttccgcc cgtgtcgggc aacccggagc cggtcaattc
      421 cccggccatt attcaggagt taacccccca tgtctgaact taccgccaaa gccctgagcg
      481 actcgcgctc gtttgagaac gccattcttg agcgggatat gcgcatgaaa aaggcgggct
      541 ggatcgtggc ggcgctcgcc ggtctgctgc tcgtcttcgc ccttgccgcc cttatcctgc
      601 tgctaccgct caaaaccacc accgtggagc tgtacacgct cgaccgtcag accgggcgca
      661 tcgagcgcgt caccaccgtc ggcgaggaca agctggacac ctccgaggtg ctcaatctct
      721 cgcagactgc gtcctacgtg aagcgccgcg aggggtacaa ctatttcgcc cttcagcagg
      781 actacagaga gacgcagatg ttcaacagcg acgaggtcaa caaggaatac ctcgactggt
      841 ttaacagccc ggacgcgccg gacgctgtgt ttcagcaggc ggcctatgta gtcacggtgg
      901 aagtcctctc caacgtaccg tcagagggca ccggctccag catggtggag atgctgcgca
      961 tcaagcgcac cattcgaaag gtcaaggaca acaccgaaac gtatgattac tggacggtgc
     1021 gcatgaccta ccgctatttc ccgcagaaga aaatgacggc ctccgagcgc gaggtgaacc
     1081 cgttcggctt catcgtcacc agttaccagc ggtttaagga gaaaagcgat gaataaggta
     1141 ctgatgtgtg ccctcctgct gaccgcagcg tcagcatggg gggcggcgac accgcgcggc
     1201 agcgcctatg acagccgcat gcagaacatc ccctacaaca gccagaacac gacagtgatt
     1261 aacacgcggg cgggttacat cacgacgctg gtttttgacg ccgacgagga ggtgatcacc
     1321 gccaccgctg gttttccgca gggctggacg atcacacccg accgtaaccg cgtggggatt
     1381 agccccgcgc cggtgaa
//
LOCUS       DADLTI010000347         1390 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLTI010000347 DADLTI010000000
VERSION     DADLTI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.353"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..573)
                     /locus_tag="JN755_004351"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="JN755_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(621..1115)
                     /locus_tag="JN755_004352"
     CDS             complement(621..1115)
                     /locus_tag="JN755_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382880.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          281 c          263 g          423 t
ORIGIN      
        1 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
       61 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
      121 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
      181 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
      241 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
      301 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
      361 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
      421 attacttaag taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg
      481 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt
      541 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaattga
      601 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
      661 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
      721 gccttgcctt cagcatcgat agcggtataa tcagagtcgt tataaagttt aacctgcgaa
      781 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
      841 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      901 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      961 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
     1021 ccgacgaagt taacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
     1081 agagtgaatg caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc
     1141 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
     1201 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tatagattta
     1261 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
     1321 tcagaatata tttatataga aagatagatg aatttattga tgtcagttat aaaaaagggc
     1381 caccggtcgg
//
LOCUS       DADLTI010000348         1370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLTI010000348 DADLTI010000000
VERSION     DADLTI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.354"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1059
                     /locus_tag="JN755_004353"
     rRNA            <1..1059
                     /locus_tag="JN755_004353"
                     /product="16S ribosomal RNA"
     gene            1116..1192
                     /locus_tag="JN755_004354"
     tRNA            1116..1192
                     /locus_tag="JN755_004354"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1150..1152,aa:Ile,seq:gat)
     gene            1235..1310
                     /locus_tag="JN755_004355"
     tRNA            1235..1310
                     /locus_tag="JN755_004355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Ala,seq:tgc)
BASE COUNT          345 a          316 c          412 g          297 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac ctccttacct taaagaagcg ttctttgtag tgctcacaca
     1081 gattgtctga tgaaaatgag cagtaaaacc tctacaggct tgtagctcag gtggttagag
     1141 cgcacccctg ataagggtga ggtcggtggt tcaagtccac tcaggcctac caaatttgca
     1201 cggcaaattt gaagaggttt taactacatg ttatggggct atagctcagc tgggagagcg
     1261 cctgctttgc acgcaggagg tctgcggttc gatcccgcat agctccacca tctctgtagt
     1321 gattaaataa aaaatacttc agagtgtacc tgcaaaggtt cactgcgaag
//
LOCUS       DADLTI010000349         1355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLTI010000349 DADLTI010000000
VERSION     DADLTI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.355"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            193..462
                     /locus_tag="JN755_004356"
     CDS             193..462
                     /locus_tag="JN755_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382881.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..571
                     /locus_tag="JN755_004357"
                     /pseudo
     CDS             459..571
                     /locus_tag="JN755_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="JN755_004358"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="JN755_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7382882.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          262 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat gggga
//
LOCUS       DADLTI010000350         1345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLTI010000350 DADLTI010000000
VERSION     DADLTI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.356"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(172..450)
                     /locus_tag="JN755_004359"
     CDS             complement(172..450)
                     /locus_tag="JN755_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382883.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(517..767)
                     /locus_tag="JN755_004360"
                     /pseudo
     CDS             complement(517..767)
                     /locus_tag="JN755_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(985..1140)
                     /gene="hokD"
                     /locus_tag="JN755_004361"
     CDS             complement(985..1140)
                     /gene="hokD"
                     /locus_tag="JN755_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7382884.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1212..>1345)
                     /gene="relE"
                     /locus_tag="JN755_004362"
     CDS             complement(1212..>1345)
                     /gene="relE"
                     /locus_tag="JN755_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7382885.1"
                     /translation="IKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          329 a          348 c          328 g          340 t
ORIGIN      
        1 agcggctggc gagcggcagg aacgaccccg gacggcagat tacgcatgct tttcggttcc
       61 ggctccacca gtaaccgggt attgtggaat accggcatgg attcacgacc gggcttaagg
      121 accaccagcc cgagttccgg aaccagaaca ggtcgaagta atacccgcac gttacctcca
      181 gatccgttgc tggaatgtgc gggacggacg cggtggctgt tcggagtaag ggagcctgac
      241 ggagattatc cagtgacggt agtcgaggct aagggctttt ttaacctcgc atccgcgcct
      301 gcggtaacac tgaatgagcc attcggcctg ttcttcagtg catgagggat gctggtacca
      361 gtctgactta aatgcgtgag aataccgctc gtgcgtgtgg gcaagaacgg tcgaattatc
      421 atgattgtaa tattttgcgt tgcgtgccat cggttttctc cggtggcacg gtgttactca
      481 gcgggagttc agccccgcgc aagattgtag atgagtttat tcttctgaaa aagcagaaaa
      541 gccagctttt attccgatct ctttcaatgc ctgtaatgaa gtgacaaact caccgtcgcg
      601 caagataaat ccgtccgtca ctcgggcatc cacaaaatta attaacgcag ccccattttt
      661 tgcaaacaca caatgcggta atgactaaca atatttccat tttcaacgca cacagcatag
      721 aggccatctt cacaaaaaat tttacgcagt tcttcgatgt tcatcatcag aatccttcca
      781 gataagtagc actcccctgt tcggggtcca tcccttttat ccctgcgcgc tacttaagta
      841 tttttgattc tattccggca ccatccagaa cttcaaatgc gttgaaaata aaaacaaaaa
      901 cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc tgccacatca gaggtggcga
      961 gggatttctc actcgccggg tctcttactc ctcaggttcg taagctgtga agacagcgac
     1021 ctccgtctgg ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt
     1081 cactatgacg gttaaacaga tgacgatcag ggcgattaac atcgcctttt gctgcttcat
     1141 agcctgcttc tcctgtcaac gcaaagcaga agtgtcacct tcggtgcgaa acagagatgt
     1201 catgctttgg ttcagagaat gcgtttgacc gcctcgctat atacttccga gcgttctctt
     1261 ttcccaacag aaatcacgaa aacgacaact ttctcgtcta taacctggta tacaaggcga
     1321 tagcctgaag accggagctt aatct
//
LOCUS       DADLTI010000351         1312 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLTI010000351 DADLTI010000000
VERSION     DADLTI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.357"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..848
                     /locus_tag="JN755_004363"
     CDS             <1..848
                     /locus_tag="JN755_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7382886.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
     gene            821..1312
                     /locus_tag="JN755_004364"
                     /pseudo
     CDS             821..1312
                     /locus_tag="JN755_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
BASE COUNT          262 a          374 c          372 g          304 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcggcga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gtctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg gtgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
     1261 ctgtggatct tagtggcagt attgagcgaa aaacggctgt tgcagcaggt ca
//
LOCUS       DADLTI010000352         1299 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLTI010000352 DADLTI010000000
VERSION     DADLTI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.358"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..123)
                     /locus_tag="JN755_004365"
     CDS             complement(<1..123)
                     /locus_tag="JN755_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7382887.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYA"
     gene            181..1206
                     /locus_tag="JN755_004366"
                     /pseudo
     CDS             181..1206
                     /locus_tag="JN755_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1179..>1299
                     /locus_tag="JN755_004367"
     CDS             1179..>1299
                     /locus_tag="JN755_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY7382888.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVS"
BASE COUNT          360 a          318 c          302 g          319 t
ORIGIN      
        1 ggcataaagg ctgtgggtgg tgatatcgag acgcgttgca acgctggcaa cagaataacc
       61 gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac gcttaccgct
      121 catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta aacccgtggc
      181 gattcacaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt gtacaccgat
      241 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa
      301 taccaacccg aatggcagtg ctgagaacct gatgtcgaat gggtgcgtca gcccccgcaa
      361 gatgcgataa ccgctcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa
      421 tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc
      481 atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
      541 tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac
      601 cccatttacg actggaaagt ggctgatatc tggacctgat atgcaaatca tcaaaatctc
      661 tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt
      721 tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg
      781 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
      841 ggacatgaca atcatttcta tgggcatcgg aaaatcctca aaccagaaca tttagactgg
      901 caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc
      961 aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt tccacaaacc
     1021 cagcaagggg acattggcgc gaaagatatt ccctcctggc ggcggatatg caaagtttta
     1081 ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat
     1141 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1201 gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1261 cgattaatga aatccgcatg gcgatccatc aggttagcc
//
LOCUS       DADLTI010000353         1286 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLTI010000353 DADLTI010000000
VERSION     DADLTI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.359"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..805)
                     /locus_tag="JN755_004368"
     CDS             complement(<1..805)
                     /locus_tag="JN755_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7382889.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="JN755_004369"
                     /pseudo
     CDS             897..983
                     /locus_tag="JN755_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(993..1073)
                     /locus_tag="JN755_004370"
                     /pseudo
     CDS             complement(993..1073)
                     /locus_tag="JN755_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          357 a          218 c          334 g          377 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gactaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tgtgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctt
//
LOCUS       DADLTI010000354         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLTI010000354 DADLTI010000000
VERSION     DADLTI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.360"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN755_004371"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN755_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7382890.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          361 a          228 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta aaaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADLTI010000355         1264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLTI010000355 DADLTI010000000
VERSION     DADLTI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.361"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            360..1109
                     /locus_tag="JN755_004372"
     CDS             360..1109
                     /locus_tag="JN755_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7382891.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          361 a          255 c          253 g          395 t
ORIGIN      
        1 atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcaa aaagttatcg
       61 gtgcgtttat cgaaaaacat atgttctact aattggagtc attgtctgta aaagcaactc
      121 aaaaatacaa gttgcttatt ccccttgttg gtaagaactt aattctaact gtttgttctg
      181 gatgttttaa acgtcctcct ttttatgctt gttcataacg gatatttaat aggtaacctt
      241 tgttttagca tcttctgatt tatttaaatt atgtgatagc gcacacaatt ttattatcca
      301 gtactttata ctcttaataa aataattata aatatttatc ctctataagg agatgttata
      361 tgaaaagaca gctttttact cttagtattg tcggggtatt ttctttaaat acctttgcat
      421 cattccctcc gggaaatgat gtgacaacaa aacctgacct ttactacctg acaaacgata
      481 atgctattga cagtctggca ttattaccgc ctccaccaca aatcggaagt attgccttcc
      541 tgaatgacca ggccatgtat gaaaaggggc gtttattgcg aaatactgaa agagggaagc
      601 tggcagctga ggatgccaat ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt
      661 ttggttctcc gataacggct aaagattcac cagaattgca taaattactg acaaatatga
      721 ttgaggatgc cggagacctg gcaacgcgtt cagcaaaaga atattatatg cgtattcgac
      781 cttttgcttt ttatggcgtt tctacgtgta atacaaaaga acaggataca ttatccagaa
      841 atggctctta tccatcaggt catacatcga ttggttgggc aacagcactt gtattgtccg
      901 aaataaatcc ggcacgccag gataccattc tgaaacgggg ttatgaactg ggggacagca
      961 gggttatttg cggttatcac tggcaaagtg atgttgacgc agcacgtatt gttggctctg
     1021 ctattgtagc aacactgcac tcaaatcctg tgttccaggc acaattacaa aaagcgaaag
     1081 atgaattcgc taacaatcag aaaaaataat gcttttagcc agtacgcctt ataaccccga
     1141 ccttcagggc taatgccagt cagttaagca actgactggc tctttttcag ggctgtgggg
     1201 tatttccagg gcctctcctt taccactctc gggaaggccc tttcccttct tgtcggtaat
     1261 ttca
//
LOCUS       DADLTI010000356         1263 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLTI010000356 DADLTI010000000
VERSION     DADLTI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.362"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            66..1259
                     /locus_tag="JN755_004373"
     CDS             66..1259
                     /locus_tag="JN755_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY7382892.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DADLTI010000357         1255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLTI010000357 DADLTI010000000
VERSION     DADLTI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.363"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..203)
                     /locus_tag="JN755_004374"
     CDS             complement(<1..203)
                     /locus_tag="JN755_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7382893.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYR"
     gene            complement(161..>1255)
                     /locus_tag="JN755_004375"
     CDS             complement(161..>1255)
                     /locus_tag="JN755_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7382894.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLSVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
BASE COUNT          246 a          359 c          343 g          307 t
ORIGIN      
        1 cgatagcgta cctcgacaaa aacggtggtc aggccttcac gcattatcag atcgatctcg
       61 ccgccacgct cgttcacgtt agcggcgata aaccgcagtc ctttgccttc cagccagcga
      121 cgcgcttgtg cttcccacgc atcgccggtc tgtttggtgg ttaactggcg gggactacct
      181 gaccttgttg gtactgtagc catgataact tcctgttaat cacgcaatcc gggttagccg
      241 tcagacttcc ggtattaccg ttgatttcaa aaccctgaac ctggcgcatt tgtgagaaat
      301 gatttgccaa cgaccaggca tcaacgccca tcgcatacat gcgagccagc gaataatcgt
      361 tgttcaccgc gctgagcgcc tgctgcatta acggcagatt accgcctgcc agcatcggga
      421 tttcgctgta ctgcaagcct tccatctcca gtcggaaatc cgggcctgcg gtgccttgtg
      481 cgctgcggga actggcgtac agcgttgcac cgctctggct accgttacgc atggcgatca
      541 tcggtttgat aaaagcgatt tcacccggcg ttgccacaat gtagaccgca tcgacacgac
      601 cgccattatt ggtgaactgg tcatcggttg gcgtggtttg cagcgttgga ttgttggtcg
      661 tcatgccgga gtcggttgtc gctctgggag taatcgggct acccgttaaa gcaataccag
      721 aaccgccgtt aacacccgcg cgtaattcgc tggtggaacc aaatttttgt tgcagaacgg
      781 tgccgccgcc cagtttctgc cactcttgcg caaacgcatt ggctacgcga tcgcccaatg
      841 cactgcgtgg gatcagcacc agcggcgctt gtttaccctg gtcacgaata tgacgcgctg
      901 catcgcgcgc ttcgtcttcc ggtgaaagcg cgaagtaaca aatattgacg cggttttcga
      961 tattctccgg ctggttcagt gccagtacgc tcagcggggt gttgctcttc agcaactctt
     1021 caacgttatt tttcagcaac ggaccaacca caatactcgc gccatcctgc tgaacctggc
     1081 ttaagatctg gctaagtggt tgtgatgagg tatcgtagat tttcagctct gcggatggat
     1141 ttgcgggtgc gcttaccgct gcggtgcttg tcgccggggc gcttacaggc accggctggg
     1201 ccgcaggctg atcaccggtc agatcgctaa ccgaggcttg tgccgggctg gcaac
//
LOCUS       DADLTI010000358         1212 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLTI010000358 DADLTI010000000
VERSION     DADLTI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.364"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            755..1212
                     /locus_tag="JN755_004376"
                     /pseudo
     CDS             755..1212
                     /locus_tag="JN755_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          299 a          285 c          370 g          258 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcaac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcaaaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg at
//
LOCUS       DADLTI010000359         1211 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLTI010000359 DADLTI010000000
VERSION     DADLTI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.365"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..333
                     /locus_tag="JN755_004377"
     CDS             <1..333
                     /locus_tag="JN755_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382895.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILI
                     REIKGSSH"
     gene            333..680
                     /gene="tnpB"
                     /locus_tag="JN755_004378"
     CDS             333..680
                     /gene="tnpB"
                     /locus_tag="JN755_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7382896.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            700..>1211
                     /locus_tag="JN755_004379"
     CDS             700..>1211
                     /locus_tag="JN755_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7382897.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPILPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCR"
BASE COUNT          289 a          338 c          329 g          255 t
ORIGIN      
        1 cggcctaatt ttccttatga atttaaaatc gctttagtgg agcagtcact gcagcccgga
       61 gcctgtgtgg cgcagatcgc ccgtgaaaac ggaatcaacg ataacctgct cttcaactgg
      121 cgccatcaat accggaaagg tggcctgctg ccttccggaa aaaatatgcc tgcactgctt
      181 cccgtgacgt taacgccgga gcagataaat acaccgtccg acagtctgtg ctgtgagctg
      241 gttctgccgg ccggaactct caggcttaaa ggtaaactga cgccggcgtt attacagata
      301 cttatccgcg aaataaaagg gagcagccac tgatgatatc tttccctgca ggttcgcgta
      361 tctggctggt tgcaggtatc accgatatgc gaaatggctt taacggcctg gcctcaaaag
      421 ttcagaacgt cctgaaggat gacccgttct ccgggcatct gttcatcttc cgcggacgcc
      481 ggggtgacca gataaaagtg ttgtgggctg acagtgacgg actgtgcctc tttaccagac
      541 gcctggagcg gggccgcttc gtctggccgg tcacccgtga cggcaaggtg caccttactc
      601 cggcccagtt atccatgctt cttgagggca tcgactggaa acacccgaaa cgaacggaac
      661 gcgctggcat ccgcatataa cccgttgtaa agtgaggata tggacacctc actttctcat
      721 gagaatgccc gcctgcgggc actgttgcag acgcaacagg acaccatccg ccagatggcg
      781 aaatacaacc gcctgctctc acagcgggtg gcggcttatg cttccgaaat caaccggctg
      841 aaggcgctgg ttgcgaaact gcagcgcatg cagttcggta aaagctcaga aaaacttcgc
      901 gcaaaaaccg aacggcagat actggaagca caggagcgaa tcagcgcact tcaggaagaa
      961 atggcggaaa cgctgggtga gcaatatgac ccgatactgc catctcccct gcgccagtct
     1021 tcagcccgta aaccgttacc tgcctcactt ccccgtgaaa cccgggtcat ccgtccggaa
     1081 gaggaatgct gcccggcctg tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag
     1141 cagctggagc ttatcagcag cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc
     1201 tgttgccggt g
//
LOCUS       DADLTI010000360         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLTI010000360 DADLTI010000000
VERSION     DADLTI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.366"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..338)
                     /locus_tag="JN755_004380"
     CDS             complement(<1..338)
                     /locus_tag="JN755_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7382898.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFA"
     gene            420..647
                     /gene="vapB"
                     /locus_tag="JN755_004381"
     CDS             420..647
                     /gene="vapB"
                     /locus_tag="JN755_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7382899.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            647..1045
                     /gene="vapC"
                     /locus_tag="JN755_004382"
     CDS             647..1045
                     /gene="vapC"
                     /locus_tag="JN755_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7382900.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1054..>1210)
                     /locus_tag="JN755_004383"
     CDS             complement(1054..>1210)
                     /locus_tag="JN755_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7382901.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
BASE COUNT          304 a          337 c          299 g          270 t
ORIGIN      
        1 gcaaaatcca cggcctggtt atgtgcttca ataagacgtt tgtccccccc gagcatggcc
       61 atcatggaga tacttttggg agcggagaag gtcaggtcgt agccgggacg atgcctgtta
      121 ctgccatcct gcatgcggct taaatccgcc ccgtccggca gcctgccctc cagaaggcgg
      181 gtaaagacat ccttatcgac actgccctgc agccccagtt gttcagcccc ctggccggcc
      241 cagcgttctc ccatgctgcc cagcacatag taattatcct tgtcggtata ataattaccg
      301 gcacttccgg ccgatctgac ctgcgcgata ctcatcataa acgtatatcc ctttgacata
      361 tcccggtatc aatcccacaa tagatataca caagacatat ccacataagg aggcaaataa
      421 tggaaaccac cgtatttctc agcaaccgca gccaggcggt cagactgcca aaagcggttg
      481 cattgccgga aaacgtaaaa cgcgttgaag tgattgctgt cggacgaacc agaatcatta
      541 cgccagccgg agagacatgg gacgaatggt tcgacggaca cagcgtcagc accgatttta
      601 tggataacag ggaacagccc ggcatgcagg aaagggagtc attctgatgc tgaagtttat
      661 gctcgatacc aacatctgca tttttacgat aaagaacaaa cccgccagcg tcagggagcg
      721 ttttaacctg aaccagggga aaatgtgtat cagttcggtc accctgatgg agctgatata
      781 tggtgcagaa aaaagccaga tgcctgaacg taatctcgct gtgatcgagg gatttgtttc
      841 ccgcattgat gttctggatt acgacgctgc tgcagccaca cacaccggcc agataagagc
      901 agaacttgcc cgtcagggac gccctgtcgg gccatttgat caaatgatcg caggtcatgc
      961 ccgcagtcgg gggctgatta ttgtgactaa taacacccgg gaatttgaac gtgtgggcgg
     1021 cctgagaact gaagactgga gctgacctgt tcatcagaaa tcatctcccg gctcaacatc
     1081 ctccccgcgt tcccggtgca cattgatgtt cacctcttca cgacgctgca tctgctgcca
     1141 ggtgtccgga tgattttcct gctgccatgc ctcataagcg gccatatcca ccacttcacc
     1201 ggattcactg
//
LOCUS       DADLTI010000361         1205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLTI010000361 DADLTI010000000
VERSION     DADLTI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.367"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            14..925
                     /locus_tag="JN755_004384"
     CDS             14..925
                     /locus_tag="JN755_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7382902.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JN755_004385"
     CDS             941..1168
                     /locus_tag="JN755_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382903.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          338 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggc
//
LOCUS       DADLTI010000362         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLTI010000362 DADLTI010000000
VERSION     DADLTI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.368"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            2..385
                     /locus_tag="JN755_004386"
     CDS             2..385
                     /locus_tag="JN755_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7382904.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JN755_004387"
     CDS             complement(386..1030)
                     /locus_tag="JN755_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7382905.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcacgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADLTI010000363         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLTI010000363 DADLTI010000000
VERSION     DADLTI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.369"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(569..748)
                     /locus_tag="JN755_004388"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="JN755_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..>1155
                     /locus_tag="JN755_004389"
     CDS             842..>1155
                     /locus_tag="JN755_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7382906.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
BASE COUNT          318 a          213 c          298 g          326 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
     1081 taaagaactc cagcgcctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     1141 cgttgaatat gggcg
//
LOCUS       DADLTI010000364         1145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLTI010000364 DADLTI010000000
VERSION     DADLTI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.370"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..171)
                     /locus_tag="JN755_004390"
     CDS             complement(<1..171)
                     /locus_tag="JN755_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382907.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1145
                     /locus_tag="JN755_004391"
     CDS             308..>1145
                     /locus_tag="JN755_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382908.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTN"
BASE COUNT          301 a          294 c          332 g          218 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatg
//
LOCUS       DADLTI010000365         1144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLTI010000365 DADLTI010000000
VERSION     DADLTI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.371"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            114..>1144
                     /gene="curA"
                     /locus_tag="JN755_004392"
     CDS             114..>1144
                     /gene="curA"
                     /locus_tag="JN755_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7382909.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
BASE COUNT          271 a          247 c          352 g          274 t
ORIGIN      
        1 ttcctcgatg aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat
       61 cccgtgagcc tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc
      121 aacaaaagca gcgtaatcga cgttgggttc tggcctcgcg tccacatggc gcaccggttc
      181 cggagaattt ccgtcttgaa gaagatgatg tcgccacacc gggtgaagga caggtgttac
      241 tgcgcacagt ttatttgtcc ctggacccgt atatgcgtgg acgtatgagc gatgagccat
      301 cttattcacc gccggttgat attggcggcg tgatggtcgg cggtacggtg agccgtgtcg
      361 tggagtcgaa tcatcctgat tatcagcctg gcgactgggt gctgggctac agtggatggc
      421 aggactatga catatccagt ggtgatgacc tggtgaaact tggcgatcat ccgcaaaatc
      481 catcgtggtc gctgggtgtg ctggggatgc caggctttac cgcttatatg gggctgctgg
      541 atatcggtca gcctaaagag ggcgaaacgt tggtggtagc tgcggcgaca ggaccagtgg
      601 gggcgacggt ggggcaaatc ggcaaactta aaggttgcag agtgatgggg gtagccggtg
      661 gcgcggaaaa atgccgccat gctaccgagg tgttaggctt cgatgtttgc cttgattacc
      721 tcgcggacga ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt
      781 atgaaaacgt gggcggtaag gtatttgatg cagtgctacc attacttaat acatctgcgc
      841 gcattcccgt ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg
      901 atcgtttacc cctgttgatg gcaacggtgc tgaaaaaacg tattcgcttg caagggttta
      961 ttatcgctca ggattatggt caccgcatcc atgagtttca gaaggagatg gggcaatggg
     1021 tgaaagagga taaaatccac taccgcgaag atattactga tggtttagag aatgcgccac
     1081 agacgtttat cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtcgcgg
     1141 gtga
//
LOCUS       DADLTI010000366         1122 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLTI010000366 DADLTI010000000
VERSION     DADLTI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.372"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>1122
                     /gene="pdeF"
                     /locus_tag="JN755_004393"
     CDS             <1..>1122
                     /gene="pdeF"
                     /locus_tag="JN755_004393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7382910.1"
                     /translation="ATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYL
                     PLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVF
                     TPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLG
                     TLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLA
                     LGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHS
                     YQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHL
                     PNVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          245 a          254 c          252 g          371 t
ORIGIN      
        1 gcaacttata taaaaatacg tgataaatgg tgggggcttc cgctgttcct gccttcttta
       61 atcttgccca ttttcgccca cattaatact ttcgcgcata tttcttccgg tgaggttttt
      121 ctcttttatc tgcctttggc actgatgatc agcatgatga tgtttttcag ctgggcggca
      181 ttgccaggga tcaccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa
      241 acgctctcat taacggctaa ttttattatc attatcattc tctgttgggg cggttacagg
      301 gtctttactc cccggcgtaa caacgtttca catggtgata cccgtttaat ttcccagcgt
      361 atattctggc agatagtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgca
      421 tttgtaggat tactggcgag cagagaaaat ctggtcggtg tcatgccttt taacctcggg
      481 actttaatca attatcaggc cttactggtg ggtaatctga tcggtgtccc gctgtgctac
      541 ttcatcattc gggtagtgcg aaatccattt tatttacgta gctattattc gcaattaaaa
      601 cagcaggttg atgccaaagt caccaaaaaa gagttcgcgc tctggctact ggcattaggt
      661 gctttattgt tgctgttatg catgccgtta aatgaaaaaa gcacaatttt tagcaccaat
      721 tataccttgt cattattgct gcccctgatg atgtggggag caatgcgcta tggttataag
      781 ctgatttcgc tgctctgggc ggtcgtgttg atgatcagca tccacagcta tcaaaattac
      841 attcccattt atcctggcta taccacgcag ctgaccataa cctcctccag ttatctggta
      901 ttctctttta ttgtcaatta tatggctgta ctggcaactc gtcagcgagc ggtagtcaga
      961 cgcattcagc ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg
     1021 aatcgcgcgt tgcgtgatgc cctctggtct gcgctttgtt atttacgcat ccctggcatg
     1081 gaaatgctgg ttaagaacta tggcatcatg ctgcggattc aa
//
LOCUS       DADLTI010000367         1114 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLTI010000367 DADLTI010000000
VERSION     DADLTI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.373"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(3..76)
                     /locus_tag="JN755_004394"
     tRNA            complement(3..76)
                     /locus_tag="JN755_004394"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:tct)
     gene            complement(78..153)
                     /locus_tag="JN755_004395"
     tRNA            complement(78..153)
                     /locus_tag="JN755_004395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Ile,seq:cat)
     gene            complement(330..1019)
                     /locus_tag="JN755_004396"
     CDS             complement(330..1019)
                     /locus_tag="JN755_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7382911.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          271 a          306 c          229 g          308 t
ORIGIN      
        1 ggcccaccac cgaggacttg aacctcggac cgtcaactta gaaggttgat gctctatccg
       61 gatgagctag tggtggttgg tggcccttgc tggacttgaa ccagcgacct ggcgattatg
      121 agtcgctcgc tctcaccact gagctaaagg gccggaggca gaataataac catatgtcat
      181 cacatctgca aactcatctg accaccagca cgtttaacgt cctgtgccat ttttcaggca
      241 taaaaaaacc gctcggcggc gggttttgct tactttacca tcgcgtacaa aatcggcaaa
      301 gtatcagatt tgcatgaaat atatgccttt taatctactt ttgcaatact ttgctttgaa
      361 aatgccgctt tttgttctga acgtgttctc atcacaaaca ataaagcctc actatccagt
      421 cggtgaaaaa tgtgtttcat tacaacccag tgagcagtaa atgttttgga ccagtttttg
      481 gttgttactc ccgccagtaa tgccagctcc tgatattcat aaccttcccc accaaaaagt
      541 tctgctttta ccgcctgcgc cgccagccag attaattttt tcaggcgttc ctgtgttttc
      601 tctgcaattt tcctagtgcc agaatgagcc ttaaactctt cccacgccca gtgggttatg
      661 gtgatctgat actcccagca aatacttccg ctgtaacacc acaacaacca ggctttatgg
      721 tgctcttcaa gagacagaac agcccgccgc cacgatgatg tcgaaaacgc aacctgactg
      781 accagaggaa ttgacgcccc cttcgccagt gattgttttc ccgggattgg tggattatcc
      841 cgcgttatca tttttccggt cacctcatcg cggtaccgga tttttttacg cctgtaacgc
      901 cctgtatcga acatggcatt ctcctgccag gcttcaagct gaccttttgt cgccccactc
      961 aaatcagcgg tggcgataat gagctgctca cgaacaaact gtaaatactg gttattcatg
     1021 cacactccag ctctgtgatt tttatcccca accgaccacc aggaacaggc agtccgcgca
     1081 caatattggt ttcatcaaac tgctcgtcgt cgat
//
LOCUS       DADLTI010000368         1112 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLTI010000368 DADLTI010000000
VERSION     DADLTI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.374"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..1034
                     /locus_tag="JN755_004397"
                     /pseudo
     CDS             1..1034
                     /locus_tag="JN755_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967842.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
BASE COUNT          344 a          209 c          166 g          393 t
ORIGIN      
        1 cttattgcct ccatatcatt aaattttaat ttaatgtatg cccctttaat ttttaacatc
       61 tccgcaatta ttgttagggc attgcttgta tctttcatac tctctggaag atttagttac
      121 gtaaacataa caccaagaat atttactgct ttgtttatta taataatgcc ggaagtatca
      181 gaagtgcacg ccaatgttac taatgattat tggtatttat cactatattt acttcttgtt
      241 atgttagctc caaagcctac aaacaattat caaaaagcac acgattatat agcgataatc
      301 atatgtggac taagtggtcc atttatagta ttcatggctc caatggtagg gataaatata
      361 cttatacaaa aacgcttatc tacaaaaaaa taaccactca cgaatacgtt ttcattgcca
      421 tttgtttgat tcaatttatt tctattatta tgacatcctc tgagaccaga gttgatatgg
      481 ttcttggagc taatttcgca acactttgca aaatattaac aacaaaggtt tttctgggtt
      541 tatgggctaa tggtgacttt ttatcacctt tatggaatca tgattcaatt tgcattgcaa
      601 ttacaatgat ttgtctttcc atcactattt ttacatttat tgtttccaat taccccatgc
      661 gctcggcaat agttttcgca atactaacat taacgttttc actagcaaaa ccaatgcttt
      721 catctacaag tgagcaatgg ccattattaa ttcatggcgg aggaagatat tctgtcatac
      781 caacaatcat atggtactct gtattggttt acttcttaaa ctatatttcc cacttgcact
      841 ttaaattcct accatggatg ttatatacaa catcgttaat ctcatgttta tattttttca
      901 atcttacccc actcccagat tacgggtgga aagagcaagt taaaaaattt gaaagcttag
      961 caccaggaga atcgtattct ttcaagttca accctgcagg atggacgatg acattaataa
     1021 aaaaacagcc ttaatgttga atggtagtta cttaatgtta ttgatctaag ttttgcatca
     1081 ataacccccc ccgaaactta ggggggggca tc
//
LOCUS       DADLTI010000369         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLTI010000369 DADLTI010000000
VERSION     DADLTI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.375"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..387)
                     /locus_tag="JN755_004398"
     CDS             complement(<1..387)
                     /locus_tag="JN755_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7382912.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JN755_004399"
     CDS             816..1097
                     /locus_tag="JN755_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382913.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          308 a          249 c          251 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctac ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADLTI010000370         1099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLTI010000370 DADLTI010000000
VERSION     DADLTI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.376"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..122)
                     /locus_tag="JN755_004400"
     CDS             complement(<1..122)
                     /locus_tag="JN755_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382914.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHL"
     gene            complement(194..1063)
                     /locus_tag="JN755_004401"
     CDS             complement(194..1063)
                     /locus_tag="JN755_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY7382915.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          292 a          317 c          230 g          260 t
ORIGIN      
        1 agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag
       61 agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc
      121 ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc
      181 cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg
      241 ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct
      301 catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc
      361 ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc
      421 gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact
      481 cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt
      541 cttcttccga gatcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga
      601 gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc
      661 cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca
      721 acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct
      781 ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt
      841 tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat
      901 cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc
      961 gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg
     1021 cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccgcat
     1081 cccctaaaac ccatacttt
//
LOCUS       DADLTI010000371         1097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLTI010000371 DADLTI010000000
VERSION     DADLTI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.377"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            101..1097
                     /locus_tag="JN755_004402"
                     /pseudo
     CDS             101..1097
                     /locus_tag="JN755_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          297 a          282 c          294 g          224 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg gttgccagtt
      481 aaatgccgga accgttcgcc gctggttgcc gtctgcgggg attgtgtggc gaagggctgc
      541 gccaactctg cgtatccgtg acccgcataa agatgaaaag atggcagcaa tccataaagc
      601 actggacgaa tgcagcgcag agcatccggt cttttatgaa gatgaagtgg atatccatct
      661 tcatcccaaa atcggtgcgg actggcaact gcgcggacag caaaaacggg tggtcacgcc
      721 gggacagaat gaaaaatatt atctggccgg agcgctgcac agcgggacag gtaaagtcag
      781 ctatgtgggc ggcaacagca aaagttcggc gctgttacat cagcctgctg aagcggctta
      841 aagcgacata ccgtcgggcg aaaaccatca cgctgatcgt ggacaactac attatccaca
      901 aaagccggga aacacagagc tggctgaagg agaacccgaa gttcaggggc atttatcagc
      961 cggtttactc gccatgggtg aatcatgttg aacggctatg gcaggcactt cacgacacaa
     1021 taacgcgtaa tcatcagtgc cgctcaatgt ggcaactgtt gaaaaaagtt cgccatttta
     1081 tggaaaccgt cagccca
//
LOCUS       DADLTI010000372         1091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLTI010000372 DADLTI010000000
VERSION     DADLTI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.378"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..949
                     /locus_tag="JN755_004403"
     CDS             <1..949
                     /locus_tag="JN755_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7382916.1"
                     /translation="LWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLA
                     VENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDA
                     MRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKKTAG
                     GRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLK
                     QHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDP
                     FECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          264 a          255 c          365 g          207 t
ORIGIN      
        1 cctgtggata gcgacacagc tgaatcgtct tcctgactgc gactgggtac acctggtctt
       61 caccctgccg gacacgctgt ggccggtgtt cgaaagcaac cggtggctgc tgaatgacgt
      121 gtgccgtctg gcggtggaga atctgctgta tgccgcccgg aaacgggggc aggaacccgg
      181 tatcttctgc gccatccaca cgtatggccg tcgtctcaac tggcatccgc atgtacatgt
      241 gtctgtaacc tgtggaggtc tgaataagca tggtcagtgg aaaaagctga gcttcctgaa
      301 agacgcgatg cgttcgcggt ggatgtggaa tatgcggcaa cggcttctga aagcgtggtc
      361 agaggggctg gcgatgccgg agtcgttgtc acatatcacg acggaatcac agaggagaag
      421 cctggtgctg aaagccggag gaaaatactg gcatgtgtac atgtcgaaaa aaacggccgg
      481 cgggaggaat acggcgcgct acctgggtcg ttatctgaag aagccgccga tagcggcctc
      541 ccggctggca cattacaacg gaggggcgag cctgagcttc cgttacctgg accacaaaac
      601 gggagaaacg gcgacggaaa cgctgacaca gcgtgagctg gtcgcgaggc tgaaacagca
      661 catcccggag aagtttttta agatggtgag gtacttcggg ttccttgcca gccgggtgtg
      721 tggagagaag ctgccgcagg tgtaccgtgc tctggggatg gataaaccgg aaccagtggc
      781 gaaagtgtgc tatgcacaaa tggtgaaaca gttcctgagt cgtgacccgt tcgaatgcgt
      841 gctgtgtggc ggccggatgg tataccgccg ggccatcgcg ggactgaatg tggaggggct
      901 gaagaaaaac gcgcgggata tcagtctgct gaggtatatg ccggcctgac acaggctaag
      961 tgtgcccggg aatcggtgca agtataaaaa acagcctgtt gcggtataag aatcgaccat
     1021 gcagagagag aaaacaccgg agtggaggga aaagcagaaa agctcacgtg gcataaggcg
     1081 tggacagcgg t
//
LOCUS       DADLTI010000373         1086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLTI010000373 DADLTI010000000
VERSION     DADLTI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.379"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..476
                     /gene="ybcL"
                     /locus_tag="JN755_004404"
     CDS             <1..476
                     /gene="ybcL"
                     /locus_tag="JN755_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7382917.1"
                     /translation="NEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVT
                     VYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGA
                     CPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
     gene            486..>1086
                     /locus_tag="JN755_004405"
     CDS             486..>1086
                     /locus_tag="JN755_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7382918.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          342 a          203 c          222 g          319 t
ORIGIN      
        1 gtaatgaaat aaaaacagga gagcaactta caacgtctca tgtattttct ggatttgggt
       61 gtgaaggtgg taatacatcg ccctcattaa cctggtctgg tgctcctgaa ggcaccaaaa
      121 gctttgccgt aactgtgtat gatccagatg ctcctacagg cagtggatgg tggcactgga
      181 ctgttgctaa tattccagca actgtaacat atttgcccac tgatgcagga agacgtgatg
      241 gtacaaaact tccgactggt gctgttcaag gccgaaatga ttttggttat gcaggatttg
      301 gtggagcatg tcctcccaaa ggagataaac cccatcatta ccagtttaaa gtatgggctc
      361 taaaaactga taagattcct gtggattcta attccagcgg agcgttagtt ggttatatgc
      421 ttaatgccaa taaaattgca accgctgaga taacaccagt ttatgagata aagtagggta
      481 agagtatgct ggcaaaagat aagtctaacc taaagattga agaaatacgg atgcataaac
      541 atcatgagat tcacagggtt aagcctctta tgccagcttt atgtcgtatc cgtcagggaa
      601 aaaaagttat caattgggag acgcatagtc taactgttga taataatcag ataatattat
      661 ttccctgtgg atatgaattt tatattgtga attatccaga agcaggtctt tatcttgcag
      721 aaatgcttta ttatcccatt gatctaattg aaaagtttca aaaattttat gcgataactg
      781 atcaaattcg taacacgaca ggtttctgct tacctcagaa ccccgagtta atatattgtt
      841 gggagcaact aaaaacatct atttcccgag gcttctcaac gcaaattcag gaacacttag
      901 caatgggcgt tctactttca ttaggagcac atcatgttaa ttgtttactt ttatcagata
      961 gtaaacaatc attaataagt cgttgttata acctaatgct atccgaacct agaacaaaat
     1021 ggacagcaaa caaggtagcg agatatctct acatttctgt ttccacattg catcgccgtc
     1081 tggcaa
//
LOCUS       DADLTI010000374         1076 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLTI010000374 DADLTI010000000
VERSION     DADLTI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.380"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            88..606
                     /locus_tag="JN755_004406"
                     /pseudo
     CDS             88..606
                     /locus_tag="JN755_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          327 a          244 c          226 g          279 t
ORIGIN      
        1 tgccggttac gccgaatctg ctgaatcaga actttacgcc cacagcacct aatcaggtct
       61 gggtcgcaga ttcagttgtc caggccttcc gaaatcaacc gactgaaggc gctggtcgcg
      121 aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg
      181 cggatacagg aagcacagga gcgaatcagc gcacttcagg aggaaatggc ggaaacactg
      241 ggtgagcaat atgacccggt actgccatct tccctgcgcc agtcttcagc ccgtaaaccg
      301 ttaccagcct cacttccccg tgccccccgg gtcatccggc cggaagagga atgctgtccg
      361 gcctgtggtg gtgaactcag tccactggga tgtgatgtgt cagagcagct ggagcttatc
      421 agcagcgcct ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg ccggtgcgac
      481 catatcgtgc aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg
      541 cttctggccc atgttgttac cggaaagtat gcagaccatc tgccattata ccgccaaagt
      601 gatctactat tccacacagc tatttagctc tctttttgtt gagctcccat gattctttca
      661 tttattgtta ctaattattt tatgttatca tgatgttaga acacgctctg ctctgtgtct
      721 ctattttatc tatcatattc tgaggattaa ctacataaca aggcgcaaga tatatatcca
      781 aaaacaaata ataccgttgc aataattcct ttcagtaaag gaggtgtgat taatttcagg
      841 ggatttcaga gtaaaaattg tacaacatat taaaatatgt tcatttcgtt ataatacaca
      901 caagaaaata ttaagttaga taatatattc taaataacca tagataaaaa cgcacataat
      961 ggggaatatt ttatgaaaat acctgaagca gtaaatcata ttaatgttca gaataatatt
     1021 gatcttgttg atggaaaaac gaatccgaat aaagcaacaa aagcattaca aaaaat
//
LOCUS       DADLTI010000375         1043 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLTI010000375 DADLTI010000000
VERSION     DADLTI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.381"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..1013
                     /locus_tag="JN755_004407"
     CDS             <1..1013
                     /locus_tag="JN755_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382919.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGSKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
BASE COUNT          243 a          230 c          271 g          299 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggcagtaaaa accgctttac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggg
//
LOCUS       DADLTI010000376         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLTI010000376 DADLTI010000000
VERSION     DADLTI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.382"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..578)
                     /locus_tag="JN755_004408"
     CDS             complement(<1..578)
                     /locus_tag="JN755_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7382920.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA"
     gene            complement(645..905)
                     /locus_tag="JN755_004409"
     CDS             complement(645..905)
                     /locus_tag="JN755_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382921.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          259 a          278 c          223 g          265 t
ORIGIN      
        1 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacggcta ccgtccggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc gtgtcgcaac ggtattcaga tattgctctg cggcttcgct cagagctggc
      301 gtatgttccc gacctactga ttcgcacagg tactcaacga agcctgatac cagttctcgt
      361 tctcgaggcg tgatcgcccc accgaccgga gtccggtaat cgaatcccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgaacgcgct taaaatcagc gtgtatccac
      481 tcgcctgttt tgatttgatg cagaaattcg caactctccg acgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      601 gttactcagc agggggtcaa ccctgcactg aattgtagat gaactcactc acattcaaaa
      661 gcagaaaagc cagccttaag tccagtctct ttcagcgccc gtaatgacgt gacaaattca
      721 tctccacaca aaagaaaacc atccgtcaca agcccatcca ccaaatgaat taacgcagct
      781 ccactcttcc tttgttgaga ctgtaaacat ttaatacggc agtggctgac aatagcgcca
      841 ttctcaacgc gcacagtata gaggccatct tcactaaaaa tttcacgtaa ttcttcgatt
      901 ttcatcaaca gaatccttcc aggtaaatag cactcccctg ttcgggggcc atccctctta
      961 tccctgcgcg ctacttaagt gtttttgatt ctattcctgc gccttctgga agttcaaacg
     1021 agttg
//
LOCUS       DADLTI010000377         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLTI010000377 DADLTI010000000
VERSION     DADLTI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.383"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..>1025)
                     /locus_tag="JN755_004410"
     CDS             complement(<1..>1025)
                     /locus_tag="JN755_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7382922.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          269 c          219 g          234 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat tttcagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADLTI010000378         1018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLTI010000378 DADLTI010000000
VERSION     DADLTI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.384"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..767
                     /locus_tag="JN755_004411"
     CDS             <1..767
                     /locus_tag="JN755_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7382923.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..>1018
                     /locus_tag="JN755_004412"
     CDS             788..>1018
                     /locus_tag="JN755_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7382924.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQET"
BASE COUNT          332 a          225 c          231 g          230 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga attcattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacaat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaac gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaaca
//
LOCUS       DADLTI010000379         1017 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLTI010000379 DADLTI010000000
VERSION     DADLTI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.385"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..273
                     /locus_tag="JN755_004413"
     CDS             <1..273
                     /locus_tag="JN755_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7382925.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1017
                     /locus_tag="JN755_004414"
     CDS             275..>1017
                     /locus_tag="JN755_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382926.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          201 g          307 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgttag
//
LOCUS       DADLTI010000380         1014 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLTI010000380 DADLTI010000000
VERSION     DADLTI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.386"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN755_004415"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN755_004415"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7382927.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          266 a          228 c          234 g          286 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcg
//
LOCUS       DADLTI010000381         1004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLTI010000381 DADLTI010000000
VERSION     DADLTI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.387"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..279
                     /locus_tag="JN755_004416"
     CDS             <1..279
                     /locus_tag="JN755_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7382928.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGERA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(524..>1004)
                     /locus_tag="JN755_004417"
     CDS             complement(524..>1004)
                     /locus_tag="JN755_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAY7382929.1"
                     /translation="DGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQ
                     RLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDG
                     RAQLDRDAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
BASE COUNT          222 a          256 c          257 g          269 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaacgcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc taggttgtgt cccttaatta cgtaagcgtt
      541 gataaaagag tcggatgcag cccagtttca ccatcgccag gtaatttctc gccgttttgt
      601 cgtaacgcgt ggcgatgcgg cgatattctt tcagccgccc aaagcaccgt tcaacgacat
      661 tacgattgcg gtacgcatca cgatcaagct gtgcacgtcc atccgatgcc attttctcat
      721 ttgactttcg ggggattact gcctttatac cgttattttt cagctcattg cgcaatgcgc
      781 gccctgaata cgctttatca gccagtaccg catgtccacg gcgtttcatg ctgccgttct
      841 gacgctgaac gccaatcccg tccagaagtc gttgcgcgaa ctggctttca tgagcttgtc
      901 cggggcttag cacgatattt aacgggaggc cacttccgtc tgttgccaga tggattttgg
      961 tgccaaaacc gccgcgagag cgacccggcc cattatctcc gtcg
//
LOCUS       DADLTI010000382         1001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLTI010000382 DADLTI010000000
VERSION     DADLTI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.388"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..1001
                     /locus_tag="JN755_004418"
                     /pseudo
     CDS             1..1001
                     /locus_tag="JN755_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          266 a          238 c          272 g          225 t
ORIGIN      
        1 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
       61 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      121 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      181 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      241 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      301 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      361 gtgcggagta aacagagtct ggagactgat gaaacgtgtc ggaataaagg ctcaggtcgg
      421 ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc gtgtcaccca acaggctcca
      481 gcgacagttc aatccggatg ctcctgatga gcgttgggta acggacataa cctacatcag
      541 gacccacgaa ggctggctgt atcttgccgt tgttgttgat ctgttctcac gcaaaattat
      601 cggctggtcc atgcaatccc ggatgacaaa ggacattgtc ctgaacgcac tgctgatggc
      661 tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat tcggatcagg gcagtcagta
      721 cacaagccat gagtggcagt cgttcctgaa atcacacggc ctggagggta gcatgagccg
      781 tcgcggtaac tgccatgata atgcggttgc agaaagtttt ttccagttgt tgaaacgtga
      841 acggataaag aaaaagatct acggaacgcg ggaagaagcc cgcagtgata tttttgatta
      901 catcgaaatg ttttataaca gtaagcgtcg gcatggttct agcgaacaga tgtcaccgac
      961 agaatatgaa aaccagtatt atcaacggct cggaagtgtc t
//
LOCUS       DADLTI010000383          977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLTI010000383 DADLTI010000000
VERSION     DADLTI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.389"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            87..>977
                     /locus_tag="JN755_004419"
     CDS             87..>977
                     /locus_tag="JN755_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7382930.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVLFPG"
BASE COUNT          258 a          230 c          243 g          246 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa ggggggcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcccag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctgga
//
LOCUS       DADLTI010000384          958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLTI010000384 DADLTI010000000
VERSION     DADLTI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.390"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..98
                     /locus_tag="JN755_004420"
     CDS             <1..98
                     /locus_tag="JN755_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7382931.1"
                     /translation="YARLGFVQLSATPNAFGAVPMFLAIQHILAA"
     gene            complement(154..951)
                     /locus_tag="JN755_004421"
     CDS             complement(154..951)
                     /locus_tag="JN755_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7382932.1"
                     /translation="MAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDR
                     EAPLALGSIYYDGEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKK
                     AKEYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCM
                     DSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTS
                     NSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT          236 a          251 c          197 g          274 t
ORIGIN      
        1 tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac
       61 ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg
      121 gcaagttttg ccttagccgg gggctgagca gcgttaccac ttttgttcaa ttgcccccgc
      181 tttcagcaac tccacatcct tgccgttaat agttgccgtt gcggcaacta ttgtggtcct
      241 tacagttgca caagattttc gctcaaattc cggtttataa agaataagag acgaattact
      301 ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc agccggttta aaccgaaagg
      361 cgcgaagcca accgtttggt taatctcctt accggatggg cgacgaatgc tgaatgtcac
      421 gcgaagattg gccacttcgc ctaatttatt ggtggtggcg aagaggctat ccatgcagga
      481 aaaaccttta tctaaaaaat tatcccgcat cccagaacgg gtaaccgtga tcacgggtaa
      541 cgtatcctca accgtttttc tgtcaatcat gtcttttcga caagtagcca gaaagtcttc
      601 cgggccacca taaatgtgga cacctttatc aaggtattcc ttcgctttct tacaatctac
      661 ctcaacatac tgaccattgg cgtacatcat gcccaacctg gaccatgcta aattcatacc
      721 ttgctgcgcg gcctgattaa atagtgcata cgctttggca taatccaccc gcacctgctc
      781 accgtcgtag tagatactcc ccagcgctaa cggagcttct ctgtcacctt gtttagcggc
      841 taactcgaac catttaatcg cttcctgaac atcaggtttg gtatcatagc gaggctgata
      901 atattcccag cctaacaggc tctgcgcgtc gatatctccc gattcagcca ttttatgc
//
LOCUS       DADLTI010000385          943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLTI010000385 DADLTI010000000
VERSION     DADLTI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.391"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(121..657)
                     /locus_tag="JN755_004422"
     CDS             complement(121..657)
                     /locus_tag="JN755_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7382933.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREMIEGKQKSWLTHCTDAEAL
                     KIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWL
                     RVAEFILYSPMREAFDYA"
     gene            complement(654..941)
                     /locus_tag="JN755_004423"
     CDS             complement(654..941)
                     /locus_tag="JN755_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7382934.1"
                     /translation="MDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATKP
                     HDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
BASE COUNT          210 a          262 c          194 g          277 t
ORIGIN      
        1 aaaaaaaccc gctcaatggc gggttctggt aaagttcatg cgcttggttc gcctcgcgat
       61 acagctttgc gaagctgtac ctgaattgaa gcagtttgtg gctcattttg caatgatttt
      121 ttaagcataa tcgaacgctt ctctcatcgg tgaatacaaa atgaactcag caacacgcaa
      181 ccactgatca acacgacgtc ggcatgtaat caacgcccac tctgggtact gttcgtttaa
      241 taactcggcc atccttctct tactcatccc tcgccccaca taacgctgac tcaggacatt
      301 tagtagtcct ggatgatccg ccagaacttc acctataaca ctatcaattt ttagtgcctc
      361 tgcgtcagtg cagtgagtta accagctttt ttgctttcct tcgatcatct cacgcaaaaa
      421 tgcttccaac tctggtttat caattcccgc ttttttcaat ctgcgcaggg cttcattgac
      481 ggctgttttt gtcatttttt gggatgccag caactggtta aacatattcc ctgacctgcc
      541 gccgccaata tacgaccagc gcccccacat gcgcagtttt ccctgaatcc agacactttc
      601 cagcgtggtg agacgaaggt gttccccgct tttgcctgta tttgttgggt aaatcacaaa
      661 tatccctcct ttctccagat ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt
      721 aattcttctt tggtgtaatc gctggttttt acccgcccgt cgattaaatc gtggcatgag
      781 ctgcaggcaa tcgctgcctg catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc
      841 agcctgtaat gcgccagcac agaagtttcg ggattgtgat tgcagtagcc aggaattctg
      901 acggtgcaca tctgcccccg cgccgcttta cgtaaatcca cca
//
LOCUS       DADLTI010000386          911 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLTI010000386 DADLTI010000000
VERSION     DADLTI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.392"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            157..822
                     /locus_tag="JN755_004424"
     CDS             157..822
                     /locus_tag="JN755_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7382935.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          288 a          191 c          200 g          232 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacactagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgcgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcggaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaaata ttgctatttt ataccagcaa tacaatcgtt aaattaaatg cccggcaaat
      841 accgggcatc aagtaattac tcaaatcgcc acgcaatatt catgccgaca ccataatttc
      901 gtccgggcgc a
//
LOCUS       DADLTI010000387          898 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLTI010000387 DADLTI010000000
VERSION     DADLTI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.393"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..129
                     /locus_tag="JN755_004425"
     CDS             <1..129
                     /locus_tag="JN755_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7382936.1"
                     /translation="SSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            126..680
                     /locus_tag="JN755_004426"
     CDS             126..680
                     /locus_tag="JN755_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7382937.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          235 a          194 c          256 g          213 t
ORIGIN      
        1 agctcatgcc acgatttaat cgacgggcgg gtaaaaacca gtgattacac caaagaagaa
       61 ttacgcctga tgcatgcaga aggtgttttt cgcacacaag aaatctggag aaaggaaggt
      121 tatttatgat ttacccaaca aatacaggca aaagcgggga acaccttcgt ctcaccacgc
      181 tggaaagtgt ctggattcag ggaaaactgc gcatgtgggg gcgctggtcg tatattggcg
      241 gcggtaagac gggaaatatg tttaaccagt tgctggcatc caaaaaattg acgaaaacag
      301 ccgtcaatga agccctgcgc aggatgaaaa aagcgggaat agagaaacct gagctggaag
      361 cgtttttgcg agagatgatc aatggcaagc aaaagacctg gctggcgcat tgtactgatg
      421 cagaggcgtt atgtattgat agagtcataa gtgaggtgct ggcagagcat cctggattga
      481 ttagtgtcct ccggcaacgg tatgaagggc gggggatgac taagcgtaaa atggctgaat
      541 tgctgaatga cgcacaccct gagtggtgtt tccggacgtg ctgcagtcgg gtagatgtat
      601 ggctaaatct tgctgaatat atgctctatc taccgatgcg tgatgcattc tcttccgggg
      661 atctaaaaac cgtttgttga ctcaatctgt tatccggggc tatattcctc acgcgccagc
      721 aaaatctggc gtcgggattg gcgtcccgga tgtttacgga gcgatatgag acgcgcccgc
      781 gtcttttttc atatcgtttg cacagtcaca ttcgcgattt atggcgggct gtgtggggga
      841 gccgaaaggc tcgccggttt ccgtacccgg ttacgccaac cctgcacagt tcgccacc
//
LOCUS       DADLTI010000388          895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLTI010000388 DADLTI010000000
VERSION     DADLTI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.394"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(110..439)
                     /locus_tag="JN755_004427"
     CDS             complement(110..439)
                     /locus_tag="JN755_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7382938.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..895)
                     /locus_tag="JN755_004428"
                     /pseudo
     CDS             complement(611..895)
                     /locus_tag="JN755_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          219 a          207 c          199 g          270 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 caatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttacgcgtt caataacacg aaatg
//
LOCUS       DADLTI010000389          885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLTI010000389 DADLTI010000000
VERSION     DADLTI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.395"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..614)
                     /locus_tag="JN755_004429"
     CDS             complement(<1..614)
                     /locus_tag="JN755_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382939.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSF"
     gene            622..846
                     /gene="tnpA"
                     /locus_tag="JN755_004430"
                     /pseudo
     CDS             622..846
                     /gene="tnpA"
                     /locus_tag="JN755_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          223 a          219 c          205 g          238 t
ORIGIN      
        1 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgtggcgag tttagccacg
       61 ccagcatcca gcccgaccat tgatgctgaa gggtgagccg gagtggatac ttcactttct
      121 gtctgaatac tgatgtacca cgtaccgcag gactggctga cagtgacatt tttcacaaca
      181 cccgtgacct gacggctatt ccggtagcgc atccagccca gtttcggcag aaaaatacgg
      241 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc
      301 tttttgaatc ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg
      361 tctttcagtg actgttgcaa tggctgtgag ggagaatctt taagccattg cgtttcagtg
      421 gcatttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      481 gcctcatgat tctcattctg acgtgccaga gcacgattga aaacgaaacg acatgcgcct
      541 gcgaagcgcc tcatttcaca ctcctgttga ccacctggtc ttaactggaa tttaaatgcc
      601 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      661 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga
      721 cgccagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt
      781 gcttattttg aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      841 attaactaac ctcccaaact ggcgatatcc agtctggtaa acagc
//
LOCUS       DADLTI010000390          867 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLTI010000390 DADLTI010000000
VERSION     DADLTI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.396"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..>867)
                     /locus_tag="JN755_004431"
     CDS             complement(<1..>867)
                     /locus_tag="JN755_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7382940.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVS"
BASE COUNT          227 a          223 c          197 g          220 t
ORIGIN      
        1 cgaaacagaa taggtggcgt aactattacc ccaccagccc gataaaggaa cagataaaga
       61 gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg tcccctgtct
      121 ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc tactgctgta
      181 gccaaattgc attaatcgtt cgacatcatc ggtatgccag tattgttgcc ggcttaatgt
      241 caggtataaa ttgccataat ctctccccaa cgactgcgtt agcgttccct ccacgcgact
      301 acgtcggtta cctgtttccc aaaagtcttc agggctgttg cgccgcgatg cccactcatt
      361 gagggtatag tagccctcgg tggagtaacg atagccaacc aggcgcaagc tagtgtctgt
      421 gtcgtcaaat agttttgaat agagaaagcg atatgactga cccgtttctg tatcatcatg
      481 gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc
      541 tttaccgaca cccagagctg cagaaccata attttcagca gcctgcatac ctccatataa
      601 agtgatcccg tgggaaaaac catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc
      661 gtcttcatcg cgcaattcac ccacgctgac atcaacatct gtcagacctt cacgtttaag
      721 aatcgccaat gaagcataag gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat
      781 ggttacgtcc aaatcgccac ctgtacttgc ggtgttgaga tcggtgatct caaaagcacc
      841 ttgtggaacg gctgactgat aaatcac
//
LOCUS       DADLTI010000391          862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLTI010000391 DADLTI010000000
VERSION     DADLTI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.397"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..390
                     /locus_tag="JN755_004432"
                     /pseudo
     CDS             1..390
                     /locus_tag="JN755_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
     gene            300..527
                     /locus_tag="JN755_004433"
     CDS             300..527
                     /locus_tag="JN755_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7382941.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            505..801
                     /locus_tag="JN755_004434"
     CDS             505..801
                     /locus_tag="JN755_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7382942.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          278 a          186 c          219 g          179 t
ORIGIN      
        1 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
       61 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa tgaagtgcga cagactgaag
      121 ataaacagca ccagaacagc gaaccggagg agggaacacc ctgcaatcag actggcgagg
      181 ataactgccc tgactgtggc gcggtgatgg gagacgcaac ttatcaggaa acttttgacg
      241 aaaaaaaacc agatgaagca caggaagaag agccgaagaa aacggaaaaa gctgatgatt
      301 tgttaccgga gaacgctggc agcgatcagc ataatgatag caataatgaa actggcgaag
      361 atgaactcaa ctggaaaaaa caggtgttga ttgcggcggt ttacggttta tgtgcgaatc
      421 ctgcgtgcat agcctatgcg cctgcaatcc ctgacatcgc aatgatgatt gccaacaagc
      481 ttgaaaattt tggagtgacc agtgatgagc gcttacccga tttttgatcg tattgaagaa
      541 atggcatggg ctcgccacta ccagcagatc gttcgtgaag aaaaagaaac ggaactggcg
      601 gacgacctgg aaaaaggtct gcctcagcac ctgtttgaat cgctctgcat cgaccatttg
      661 caacgccacg gtgccaataa acaggcaatc agccacgcat ttgatgatga tgttgagttt
      721 caggagcgca tggcagaaca catccggtac atggctgaaa ccattgccca ccaccaggtt
      781 gatattgatt tggaggacta aaatctatgg cgctttttca acgagcaata aacacacagg
      841 cctatctaaa agcaggaatt at
//
LOCUS       DADLTI010000392          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLTI010000392 DADLTI010000000
VERSION     DADLTI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.398"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..761
                     /locus_tag="JN755_004435"
                     /pseudo
     CDS             1..761
                     /locus_tag="JN755_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          185 c          202 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADLTI010000393          831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLTI010000393 DADLTI010000000
VERSION     DADLTI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.399"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..831
                     /locus_tag="JN755_004436"
                     /pseudo
     CDS             1..831
                     /locus_tag="JN755_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          252 a          169 c          198 g          212 t
ORIGIN      
        1 gcattggagc tgtcaaaaga ccgacgcatt gaggatcttg aaaggcaagt tcgatttctt
       61 gaaacgcggc ttatgtatct aaaaaagctg aaagccttag ctcatcccac gaaaaagtga
      121 aagtactcaa cgagctaagg cagttttatc ctcttgatga gcttctcaga gctgcggaga
      181 taccgcgcag tacgttttat taccatctaa aggctctcag caagcctgac aagtatgcgg
      241 acgttaaaaa gcgtattggt gagatttatc acgagaatag aggccgatac ggataccgta
      301 gggtaacgct gtctcttcat cgagaaggga aacagattaa ccataaagct gttcagcgcc
      361 tgatgggaac cctctcactt aaagcagcga ttaaggtcaa gcgataccgc tcttacagag
      421 gagaggtagg gcaaaccgcc cctaatgttc tccaaagaga tttcaaggct acgcggccaa
      481 acgagaagtg ggttaccgat gttactgaat ttgcagtcaa tgggcgcaag ctgtatttgt
      541 ctccagtaat agatctcttc aacaacgaag ttatttctta cagcctttcg gaaagaccag
      601 tgatgaacat ggttgagaat atgctcgatc aggcattcaa aaagcttaat cctcacgagc
      661 atcctgttct gcactctgac cagggatggc agtatcgtat gagaagatat caaaatatcc
      721 ttaaagaaca tggtattaaa caaagcatgt ccagaaaagg caattgtctg gataatgctg
      781 tggtggagtg tttctttgga accttaaagt cggagtgttt ttatcttgat g
//
LOCUS       DADLTI010000394          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLTI010000394 DADLTI010000000
VERSION     DADLTI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.400"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..326
                     /locus_tag="JN755_004437"
     CDS             <1..326
                     /locus_tag="JN755_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7382943.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JN755_004438"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JN755_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7382944.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          255 a          181 c          153 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca agcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADLTI010000395          801 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLTI010000395 DADLTI010000000
VERSION     DADLTI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.401"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..244)
                     /locus_tag="JN755_004439"
     CDS             complement(<1..244)
                     /locus_tag="JN755_004439"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7382945.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
     gene            complement(241..663)
                     /locus_tag="JN755_004440"
     CDS             complement(241..663)
                     /locus_tag="JN755_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7382946.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT          222 a          219 c          151 g          209 t
ORIGIN      
        1 attgagtacc aaaatcacta atggatttac tataacgaat acgatattta ttcccataat
       61 ataaattcgc aatcgaacgc gtaatatctg tcgataaact ccctaacagc cccagagaaa
      121 tgccaatacc cgtagatatt gcattaaaat gagtggcgct ttgaatccct ccatacaccg
      181 tgacgccata aggaagcccg tacatcgccg tgatttcgcc cacggagaca ggggagcttt
      241 ttataccttc ttctttatat tcccccccat cacactatat ttgaaatatc cttcatgcag
      301 agcgatggcc ggcgtttgaa atggcacgga aaaatgctgc tcctcgccat tactctctct
      361 cactgtaaca tcaagctccc cgccaacggc cagcgttggt aaattatcca gcgcaaaagg
      421 ccctggcggc acagaacggg taaaaataat ttgcccattt tgtttaataa taacctgcgc
      481 ctcactttgt gctattccat gcaccacagg tgtgtagtag ctgtcctccg gcggagtcat
      541 cgcatcgtcg gtcgctaaca tcccgccgcg aaaaggtatg ctgtcaaaca cctcagacga
      601 cgtataagat tctccaagcg ttaatcggct tttaatcgca tttatgcctc gctcaaacca
      661 catgtaaccc ctttgccatc tttctgactg gccaagttcc ttgctccatg tcactgaact
      721 acgaaacctc caggcaccaa gattaaaccc tggagtgaac ctgccataac gagaattacg
      781 cgtggtagat tgattatcat t
//
LOCUS       DADLTI010000396          798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLTI010000396 DADLTI010000000
VERSION     DADLTI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.402"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>798
                     /gene="narH"
                     /locus_tag="JN755_004441"
     CDS             <1..>798
                     /gene="narH"
                     /locus_tag="JN755_004441"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7382947.1"
                     /translation="KNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDV
                     NGKIRPRLGSKMGVITKIFANPVVPQQIDDYYEPFTFDYEHLHSTPEGKHIPTARPRS
                     LIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLPRLCEH
                     CLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKSEKC
                     IFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDL"
BASE COUNT          212 a          207 c          225 g          154 t
ORIGIN      
        1 aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc
       61 aaaccgggca ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg
      121 gtccgcgatg tgaatggcaa gatccgtccg cgtctgggca gcaagatggg cgtaataacc
      181 aaaatcttcg ccaacccggt ggtgccgcag cagattgatg attactacga acctttcacc
      241 ttcgactacg aacatttgca tagcacaccg gaaggcaaac atatccctac tgctcgcccg
      301 cgttcactga ttgacggcaa acggatggac aaagtgatct ggggcccaaa ctgggaagaa
      361 ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag
      421 gagatgtacg ggcagtttga aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac
      481 tgcctcaatc ccagctgcgt ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa
      541 gacggcattg tgctgattga tcaggataaa tgccgtggct ggcgtttgtg cattagcggt
      601 tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa atgtatcttc
      661 tgttatccgc gaattgagtc aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc
      721 atccggtatc tgggcgtgct gctttacgac gccgaccgca tcgaggaagc ggcgagcacc
      781 gagcgcgaag ttgacctc
//
LOCUS       DADLTI010000397          764 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLTI010000397 DADLTI010000000
VERSION     DADLTI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.403"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>764
                     /locus_tag="JN755_004442"
     CDS             <1..>764
                     /locus_tag="JN755_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382948.1"
                     /translation="RYLNDRLTLLETQLATVNRMATANELPDAIITESGLKITPLDAA
                     VPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAKDKNLLLTTIL
                     ADAINLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQFRHPFAEHWGD
                     GTTSSSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDS
                     TYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHL"
BASE COUNT          165 a          268 c          215 g          116 t
ORIGIN      
        1 accggtacct gaacgaccgg ctgacgctgc tggaaacaca gcttgccacc gtcaaccgta
       61 tggcgacggc caacgagctg ccggacgcca tcatcaccga gtcaggcttg aagatcacgc
      121 cgctcgacgc ggcggtaccc gacaccgccc aagcgctgat cgaccagacg gcaatgatcc
      181 tgccgcacgt caagatcacc gaactgctgc tggaggtgga cgaatggacg ggcttcactc
      241 ggcatttcgc gcatctgaaa tcgggcgacc cggccaaaga caagaacctg ttgctgacca
      301 cgatcctcgc cgacgcgatc aacctgggcc tgaccaagat ggcggagtct tgccccggca
      361 cgacctacgc caagctggct tggctgcaag cctggcacat ccgcgacgaa acctacgggg
      421 cggcgctggc cgatctggtc aacgcacagt tccgccatcc cttcgccgag cactggggcg
      481 acggcaccac ctcatcgtcg gacggccaga acttccgcac cggcagcaag gccgagagca
      541 ccggccacat caacccgaaa tacgggagca gcccagggcg gacgttctac acccacattt
      601 ctgaccagta cgcgccattt cacaccaagg tcgtgaacgt cggcgtgcgc gattcgacct
      661 acgtgctcga cggcctgctg taccacgagt ccgacttgcg gatcgaggag cattacaccg
      721 acacggcggg cttcaccgat cacgtcttcg ccctgatgca cctc
//
LOCUS       DADLTI010000398          760 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLTI010000398 DADLTI010000000
VERSION     DADLTI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.404"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            30..760
                     /locus_tag="JN755_004443"
                     /pseudo
     CDS             30..760
                     /locus_tag="JN755_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
BASE COUNT          186 a          187 c          229 g          158 t
ORIGIN      
        1 ctaaacttcc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt tcccccgaag
       61 tccgtcaacg ggcagtccgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg
      121 cgacaatttg ttccattgct ccaaagattg gctgtacgcc ggagactctg cgtgtctggg
      181 ttcgccagca tgagcgggat accgggggcg gtgatggagg gctcaccacc gctgaacgtc
      241 agcgtctgaa agagctggag cgtgaaaatc gtgaactgcg ccgcagtaac gatatccttc
      301 gccaggcttc cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaatgatgcc
      361 actgctggat aagctgcgtg agcagtacgg ggtcggaccg ctatgcagcg aactgcatat
      421 tgccccgtca acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc
      481 ccgtgcgcag cgcgatgact ggctgaagaa agagatacag cgcgtatacg atgaaaatca
      541 caaggtatac ggtgtgcgta aagtctggcg tcagttgtta cgggaaggta tcagagtggc
      601 cagatgcact gtggcacgtc tcatggcggt tatgggactt gccggtgttc tccggggtaa
      661 aaaggtccgt acgaccatca gccggaaagc cgttgccgca ggcgaccgcg taaaccgtca
      721 gttcgtggca gaacgacctg accagctgtg ggtggctgat
//
LOCUS       DADLTI010000399          745 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLTI010000399 DADLTI010000000
VERSION     DADLTI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.406"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..117
                     /locus_tag="JN755_004444"
     CDS             <1..117
                     /locus_tag="JN755_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382949.1"
                     /translation="GGQFLLTTSFLASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            117..572
                     /locus_tag="JN755_004445"
     CDS             117..572
                     /locus_tag="JN755_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY7382950.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            575..>745
                     /locus_tag="JN755_004446"
     CDS             575..>745
                     /locus_tag="JN755_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7382951.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNL"
BASE COUNT          212 a          178 c          189 g          166 t
ORIGIN      
        1 ggtggccagt ttttgttgac cacttcattt cttgcctcaa atggcagtac gttaactatt
       61 cagtcaaatg gtgcggcgtt tactgccaca accatcaaga taatcgcgga gcagtgatga
      121 ttcagttcaa accaacgcga aacatcgacc tgatcgaagc agtaggaaat cacactgaca
      181 ttatcgccgg gagcaacaac ggcgatggat acgactacaa gcctgaatgc cgttactttg
      241 aggttaacgt gcacgggcag ttcggcggaa ttgtttacta tcaggagatt cagccgctaa
      301 cctttgattg ccacgccatg tacctgccag agattcgtgg attcagcaag gaaatcgggc
      361 tggcgttctg gcgatacatt ctgactaaca ccaccgttca gtgcgtcaca tcgttcgctg
      421 cgcgcaaatt ccgccacggt cagatgtact gcgcaatgat tggccttaag cgtgtaggaa
      481 ccatcaagaa atacttcaaa ggcgtggatg acgtgacgtt ttacagcgcc acacgcgaag
      541 aactaatcga cttcctgaat cacgggagat agccatgtta tatgcattta agctgggcag
      601 aaaactgcgc ggcgaggaac cttattgccc tgaaaaaggc gggaaaggtg gcagctctga
      661 taaaagcgca aagtatgcag cagaagctca gaagtatgcc gcagacctgc aaaatcagca
      721 gtggcagacg atcatgaaaa acctt
//
LOCUS       DADLTI010000400          736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLTI010000400 DADLTI010000000
VERSION     DADLTI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.407"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(12..374)
                     /locus_tag="JN755_004447"
     CDS             complement(12..374)
                     /locus_tag="JN755_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7382952.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAIRYMLYVGFMGILSAIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          222 a          143 c          160 g          211 t
ORIGIN      
        1 aagagatatt ttcatttcgc atccctaaaa acaatgaact ttgaatagac gaaaccgcac
       61 accagactga tggcggagaa ggtgataaga gtaactattg gaggaagtga gcatttatca
      121 gcagcccaac caacaatcgc actcaatatt cccatgaatc ccacgtataa catgtagcgt
      181 atcgctgtag tcgatgcttt gaatgtgaat cttgcattcg cgaagaagct aaagctcaca
      241 gctactacga aacctgtgaa gtttgccagg gcctgactgg tatgcgcggc atagatacat
      301 acaccaaaaa ctacccagtg tataagtgta ttcagcacac ctatagatgt gtactttaca
      361 aataacttta acatttattt aatcaatgag ttctgaaagt catgaagtct atcatccaag
      421 tctcaattga tcgatatttg ctgtgtctga tgagacaaaa ctgagacaca taaggcctcg
      481 caatggcttg caaggcttta catgttttga tttagtggga cgtgtgagcg cagtgttgat
      541 ggggtaatgc tttgaattag aagcggattc ttataattcg taatgcgaag gtcgtaggtt
      601 cgactcctat tatcggcacc atttaaatca ataagttaca catcattagt accttcctta
      661 tttcttgact gggacaaatt tgggaccgat gggttcagaa tcgagtctat ttgccgtgcg
      721 tgttcggtaa ggtgat
//
LOCUS       DADLTI010000401          730 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLTI010000401 DADLTI010000000
VERSION     DADLTI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.408"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..200
                     /locus_tag="JN755_004448"
     CDS             <1..200
                     /locus_tag="JN755_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382953.1"
                     /translation="PTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JN755_004449"
     CDS             188..355
                     /locus_tag="JN755_004449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7382954.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..>730
                     /locus_tag="JN755_004450"
     CDS             368..>730
                     /locus_tag="JN755_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7382955.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKN"
BASE COUNT          211 a          161 c          176 g          182 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat
//
LOCUS       DADLTI010000402          728 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLTI010000402 DADLTI010000000
VERSION     DADLTI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.409"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(55..606)
                     /locus_tag="JN755_004451"
     CDS             complement(55..606)
                     /locus_tag="JN755_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY7382956.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQPDYKIAYE"
BASE COUNT          211 a          148 c          140 g          229 t
ORIGIN      
        1 ccacttatca ttaatccgtt aatgatgtgg ccgggcgacc ccggcctaat gtgattactc
       61 gtaagcaatc ttatagtcag gctgagcgta aacgttgccc ggggtagcat ctttgcccgg
      121 ggattccata cgagctttga agttcagttc ctgatagctg ttagtattgt tgaatgtaat
      181 tactaccgga gtacctaaag tcacttcgcc gctctggcta tccagaatac gtacgccaac
      241 gcctgtagca ccaccgattt cagtttcttt ggtgtttttc agcaggtcag tctgacccgt
      301 catagtattc gctgtggtga aagtaacctt cgcttttgaa taagtggttc caccctggct
      361 caacatgcag tcttgcaggt gaatagtgaa aggttcagcc aaagatttct gatcgctttt
      421 cagtacggta tctgctactt cacccagatt tacagttaaa tcttcgtcac ctggtttaat
      481 agaacatgga gcagagataa cttcaccttt aaatttaaca ataccgtgac cttcatcagc
      541 aaacacgtta ttggaaagaa tcattgctac accagcaaca agaagtgact ttttaaaagc
      601 agacataata ttcctttata taaagataag taaaaaagag aatgattagt aactgttatt
      661 taaggactac caatcattaa aatattatat tttgttttgt gatttttata aaattagttt
      721 atctaaat
//
LOCUS       DADLTI010000403          724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLTI010000403 DADLTI010000000
VERSION     DADLTI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.410"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>724
                     /locus_tag="JN755_004452"
     CDS             <1..>724
                     /locus_tag="JN755_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7382957.1"
                     /translation="PVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEA
                     ARTRPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPALERVLG
                     PRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVV
                     VPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAAR
                     LLTEVACRAFASAAHLAAYAGLA"
BASE COUNT          142 a          249 c          190 g          143 t
ORIGIN      
        1 tacctgtcgc cgttgcccgc tcagaaggag tccttgtcgg atacctccct ggactggcca
       61 tgcgccgcat agccgactta cacgccggtg aagctaaaac tgatgctcgt gacgctgcca
      121 tcattgccga agctgcccgt acccggcctc acgcgctacg cacgctgaaa ctggctgacg
      181 agcaaatcgc cgaactctcc atgctctgcg gcttcgatga tgatcttgcc gcacagacaa
      241 cgcaggccag caaccgtatc cgcggccttc tgacccagat acatccggca ctggagcgcg
      301 ttctcggtcc gagacttgag cacccggcgg tactcgatct tctccagcga tatccctcac
      361 cagaaaaact cgcttcgctg ggtgagaaga agctggcagc ccagctctgc aaacttgcgc
      421 ctcgtctggg taaacgcctt gcagcagaca tagctcaggc actggccgaa caaaccgtcg
      481 tcgttcccgg cacgaatgcc gctgccgtag tactgccacg tctggcactc cagctcatca
      541 cgctgcgtaa gcaaagagac gaggtggcgc ttgaggtaga acagcgagtt cttgctcacc
      601 ctctttaccc ggtcctgacc agtatgcccg gagtcggtgt caggaccgca gccagactcc
      661 tcaccgaggt cgcctgccgc gccttcgcct ctgccgcaca tctcgctgct tatgctggcc
      721 ttgc
//
LOCUS       DADLTI010000404          712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLTI010000404 DADLTI010000000
VERSION     DADLTI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.411"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            278..>712
                     /locus_tag="JN755_004453"
     CDS             278..>712
                     /locus_tag="JN755_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7382958.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          217 a          123 c          147 g          225 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cg
//
LOCUS       DADLTI010000405          708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLTI010000405 DADLTI010000000
VERSION     DADLTI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.412"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(5..256)
                     /locus_tag="JN755_004454"
     CDS             complement(5..256)
                     /locus_tag="JN755_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7382959.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          195 a          190 c          164 g          159 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gcccggaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggta
//
LOCUS       DADLTI010000406          708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLTI010000406 DADLTI010000000
VERSION     DADLTI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.413"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            508..>708
                     /locus_tag="JN755_004455"
     CDS             508..>708
                     /locus_tag="JN755_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382960.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSI"
BASE COUNT          255 a          124 c          143 g          186 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa atgtattacg cgtaacaaac tcatcttctt ctggcataag tgaaaaacat
      661 ttagatcact gtgctaatac tgtgaaaaac tttttaagga agtctata
//
LOCUS       DADLTI010000407          705 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLTI010000407 DADLTI010000000
VERSION     DADLTI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.414"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..434)
                     /locus_tag="JN755_004456"
     CDS             complement(<1..434)
                     /locus_tag="JN755_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7382961.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            442..>705
                     /gene="tnpA"
                     /locus_tag="JN755_004457"
     CDS             442..>705
                     /gene="tnpA"
                     /locus_tag="JN755_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7382962.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNS"
BASE COUNT          173 a          174 c          165 g          193 t
ORIGIN      
        1 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcagcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg
      181 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttatttccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      481 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga
      541 cgccagattt ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt
      601 gctgattttg cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      661 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagc
//
LOCUS       DADLTI010000408          695 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLTI010000408 DADLTI010000000
VERSION     DADLTI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.415"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            43..>695
                     /locus_tag="JN755_004458"
     CDS             43..>695
                     /locus_tag="JN755_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7382963.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
BASE COUNT          169 a          184 c          185 g          157 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg
      481 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
      541 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg
      601 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
      661 gcactacgca aaggccaggc ctcagcattt tatta
//
LOCUS       DADLTI010000409          685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLTI010000409 DADLTI010000000
VERSION     DADLTI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.416"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..604
                     /locus_tag="JN755_004459"
     CDS             <1..604
                     /locus_tag="JN755_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7382964.1"
                     /translation="GKTVQKIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNL
                     AFVVRFSYPDDTRRQNDAARLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAG
                     SESITPDFAYDDGRFTYLGFSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSL
                     SPRFVLRYGSQVVGIENGSYGKVTVANGSTVSPDVKLESK"
BASE COUNT          159 a          207 c          206 g          113 t
ORIGIN      
        1 cggcaaaacc gtccagaaga ttttcacccc cacgcagcag gactggcaga ccaacctgtt
       61 tgtgaccacc acaaagcgcg actacagcct gatgctgaac gtgatcgata acgatcgcga
      121 ggcgggaaat ctggcctttg tggtgcgctt cagctacccc gacgataccc gcaggcagaa
      181 cgacgcagcc cgcctcgccc gtcagcagga gcttcaggcc gcgcaggaga aagaacgcat
      241 tcagtttgcc ctgaagaaat ccactgcgcc gcgcaactgg aactacaccc gcagggtggc
      301 ggcaggttcg gagtcgatca ccccggattt tgcgtatgac gacggccggt ttacctatct
      361 cggcttttca ccgcagaagc tgatcccgtc gccgaccgtc atcgtcggcg gcagcgagca
      421 ggtgatcacc ccgaccttca gcaccgaggg taactaccgg gtgatggtac tgcgctctct
      481 ttctccacgc tttgtgctgc gctacggcag ccaggtcgtg gggattgaaa acggcagtta
      541 cgggaaagtc accgtggcga acggcagcac cgtctcaccg gatgtgaaac tggagagcaa
      601 atgatgaacg aacaggaaga cattaaaacc gccgccgaac tggaggcgga agcccgcgag
      661 cgggcgcggg cagagattga cggcg
//
LOCUS       DADLTI010000410          683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLTI010000410 DADLTI010000000
VERSION     DADLTI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.417"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            36..>683
                     /locus_tag="JN755_004460"
     CDS             36..>683
                     /locus_tag="JN755_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY7382965.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG"
BASE COUNT          160 a          172 c          182 g          169 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggc
//
LOCUS       DADLTI010000411          671 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLTI010000411 DADLTI010000000
VERSION     DADLTI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.418"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(182..655)
                     /locus_tag="JN755_004461"
     CDS             complement(182..655)
                     /locus_tag="JN755_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7382966.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          173 a          162 c          142 g          194 t
ORIGIN      
        1 cctgcgaata tgtgagtttt gacctaatga gccacaaaaa ctcattttcg cccacgataa
       61 tttttctact tcctttcttt ggaatgctca tttcaatatt cacataacgt cgctatcaat
      121 cgcggaaaag gcgtagtgcg cgaagcgaac gaagcttttt gccgtcgatt gcatagcttt
      181 gttaaccctt tttccaaatt tgatagcaat agttaatgtt tgaagtaaag tgttgctcaa
      241 aaacaacttc gaaggtattt ggaatactcg ggaagaaaac atccccctct ggctcgatgt
      301 cgatcgtcga taagtggagc gtagaggcca tgggtaatgt ttctcggtaa atttctccgc
      361 caccagacac tataacgtga ccggtgaatt cagctagcct gtccatggcc tcttcgattg
      421 actgaaatac aactacattg tcatcatttg atgtccaacc tgagcgggta acgaccgcgt
      481 atttcctatt ggggagtgcg cccatagatt caaacgtctt gcgacccacc agaagccact
      541 gattgtaggt caatgcttta aaaagtagct gctccccttt cgcggaccag ggtatgtctg
      601 gaccgcaacc aatcacgccg tttttcgctt tcgcagccat caatgatact ttcaaggttc
      661 tcatcctggg t
//
LOCUS       DADLTI010000412          670 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLTI010000412 DADLTI010000000
VERSION     DADLTI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.419"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
BASE COUNT          176 a          166 c          170 g          158 t
ORIGIN      
        1 ctgggaccac ggtgccacct gtatcgccgg tcaggttatc tgcctgggac cacggtccca
       61 cctgcatcgt cggcacatta ttagttaggg acaacggttc aatcaatgcc tgtcacgaac
      121 aggtattggc ttgtcgccag caacccgttg agcggagtaa tcacgcagca ggacggctgt
      181 tacaggcgat ggtgaaagct ctgttgggat ctgaacgaag catgttgagt gaaatttgca
      241 aaatttcgca gagtatgcgg cttcatcttt aaatttccgc cagcattttt aaagccagct
      301 accgacgctg cgcgttggcc ggcccttcgt ccggcgggta accagtaccg gccgatctta
      361 cgaccctgga tgaacatcat cccgtcagca gcgtctccac tgtgatgtcg tttttcacac
      421 tgtccgatca caaaatagaa cgggcaggga caacatatgg taggaaaagc agctgccaga
      481 gacacaatat attgattacc cacagtaacg ggggataacc atgtgggtaa catattgaaa
      541 gtgcaacacg ttatctgatt aattgacttt gtaaacgccg ctgaaccatg aaaaaaaacc
      601 gggggaatca tcacagcgca taaacatacc gttttgtttt tgggtgacgg caaggaggcc
      661 ccttttatca
//
LOCUS       DADLTI010000413          669 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLTI010000413 DADLTI010000000
VERSION     DADLTI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.420"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..255
                     /locus_tag="JN755_004462"
     CDS             <1..255
                     /locus_tag="JN755_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7382967.1"
                     /translation="NDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDK
                     EKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            279..>669
                     /locus_tag="JN755_004463"
     CDS             279..>669
                     /locus_tag="JN755_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7382968.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQD"
BASE COUNT          181 a          150 c          192 g          146 t
ORIGIN      
        1 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
       61 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      121 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      181 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      241 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      301 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      361 taaacacccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      421 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      481 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      541 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      601 aaatggtggc gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
      661 cgcaggatg
//
LOCUS       DADLTI010000414          663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLTI010000414 DADLTI010000000
VERSION     DADLTI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.421"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>663
                     /locus_tag="JN755_004464"
     rRNA            <1..>663
                     /locus_tag="JN755_004464"
                     /product="23S ribosomal RNA"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DADLTI010000415          662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLTI010000415 DADLTI010000000
VERSION     DADLTI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.422"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..391
                     /gene="ospC4"
                     /locus_tag="JN755_004465"
     CDS             <1..391
                     /gene="ospC4"
                     /locus_tag="JN755_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7382969.1"
                     /translation="NYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQD
                     VAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLD
                     NAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          226 a          110 c          132 g          194 t
ORIGIN      
        1 taattatgaa gagattaaca ataaagtgac tgataaaaaa atggctcatc aggcccttgc
       61 ttattcttta ggcaataaaa aagctgatat agcactttat ttactatcta aatttaattt
      121 tacaaaacaa gatgtcgcag agatggagaa aatgaacaac aatagatatt gtaatctgta
      181 tgatgttgaa tacttactta gtaaagatgg tgccaactat aaagtgctag aatattttat
      241 caataatgga ttggttgatg taaacaaaaa atttcaaaaa gcaaatagtg gggacactat
      301 gcttgataat gcaatgaaaa gcaaagattc aaaaatgatt gattttttat taaaaaatgg
      361 agcaatatta ggcaaacgat ttgagatatg acctaaaagc tcggccaaat aataatgtcg
      421 tcgattatcg aagtcatcat gagtgattct gtccccacag tgcagcatca gcatgacagt
      481 gaattgtctg gcgtggtcca tttaatgaga tggagatgct ccctctttcc taaatagctg
      541 tgcctaatac cgctacactt ttgccagccc atgtttgcct ccggggaatg ggctgacggt
      601 tccttcaaaa tccattgcac gcagttatgt cggagcggga acttgactcc tgtctttacg
      661 gg
//
LOCUS       DADLTI010000416          660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLTI010000416 DADLTI010000000
VERSION     DADLTI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.423"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            104..>660
                     /locus_tag="JN755_004466"
     CDS             104..>660
                     /locus_tag="JN755_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7382970.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDS"
BASE COUNT          236 a          121 c          111 g          192 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacaaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgccttg cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatggtttct ggaaacagat tagacagcat
//
LOCUS       DADLTI010000417          659 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLTI010000417 DADLTI010000000
VERSION     DADLTI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.424"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(152..658)
                     /locus_tag="JN755_004467"
     CDS             complement(152..658)
                     /locus_tag="JN755_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7382971.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          161 c          146 g          179 t
ORIGIN      
        1 cgtttaacgc cctgtgccat ttttcaggca caaaaaaacc cgcataaagc gggttctttc
       61 aagtgtccat gtctgctatt cgcctcgcga tacagctttg cgaagcttac cggaattgaa
      121 gcagtttatg tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt
      181 tatctctgcg aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg
      241 aaataacaat atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat
      301 aacagagaac tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcccttt
      361 gactgaaata gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca
      421 ttttggcgtg gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg
      481 aaactggatt ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa
      541 ctttgaaaaa gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa
      601 tacgcaccgg ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatc
//
LOCUS       DADLTI010000418          652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLTI010000418 DADLTI010000000
VERSION     DADLTI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.425"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            29..373
                     /locus_tag="JN755_004468"
     CDS             29..373
                     /locus_tag="JN755_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382972.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            424..612
                     /locus_tag="JN755_004469"
     CDS             424..612
                     /locus_tag="JN755_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7382973.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
BASE COUNT          236 a          129 c          144 g          143 t
ORIGIN      
        1 tcttaacctg ggaggtgaac accccggcat cgatacctca cgggataaca gtgaattatt
       61 tgccaacgac ggtacaccga catggctaaa agaaaagcgt gcaaaagaaa tcgcacttga
      121 tgaaattatc gagttgctca acaagcacca tgggggcacc agtaacgacg ccaaacgcgc
      181 aaaagctgat ttgctggaaa aaacattcac ctcccgctca tgggaaagaa tcaaaggtat
      241 ggactggccg acaatcaaag ctggtcgtaa tgcactatgg attgaactgg aaggcgttga
      301 atatgcattc ccggatccac aaacacaaaa cgaaacgcaa caagcaggat acgatgagaa
      361 tattccggtg taaaaccaag tgcattttga acacggtttt tgaaaatatt aatggaggtg
      421 ttcatgtata aaattacagc cactattgaa aaggaaggtg gttctcctac cagctggaca
      481 agatattcaa aatctaaact aacgaaatca gaatgcgaaa aaatgctctc agggaaaaaa
      541 gaagcaggcg tttccagaga gcagaaagta aaactgataa attttaattg cgagaaactt
      601 ctgtcctcgt gagttgcatt atatcaaaat tagaacttca tagctgattg ag
//
LOCUS       DADLTI010000419          647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLTI010000419 DADLTI010000000
VERSION     DADLTI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.426"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..161
                     /locus_tag="JN755_004470"
     CDS             <1..161
                     /locus_tag="JN755_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7382974.1"
                     /translation="FGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRP
                     KYIIKDKK"
BASE COUNT          211 a          106 c           99 g          231 t
ORIGIN      
        1 tctttggtaa cccagtacgc gggtatccct ccctgcttgt atcaatactt ttcttgggtg
       61 gcgtgcaact gatcgggatt ggtgttctcg gagaatatat aggcagaata tatattgaag
      121 taaaaaatag acctaaatat attattaagg ataagaaatg attaaatcaa attacatatg
      181 ctttcttttg ctttttttat tggtttaact attcttcatt taaatcaata tacactagta
      241 tcagatgaca tttggttttc cgagaaatca atatcagaac cggatcacat caagtggctc
      301 atagatagat atttcacatg gtcatcacga acatagattg aatatgcatt aataaatctt
      361 attaatcatt tttatgcttg gtctttaatt aatgcagcaa tgttttcatc attaattatt
      421 ggcatttgct ctatctttca gagaaaaaac aacctttact ataaaataaa cacttcaatc
      481 atgtcattat tactgattgt tatgatgcca agcgatgttt ttttcaatgc cgcgatttgg
      541 ataacaggat cattcaacta tctgtggcca agtgctattg catttattgg ttattctcta
      601 cttataaaaa aaatcaatat ggaaaaccac gagaaaactc aatctct
//
LOCUS       DADLTI010000420          640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLTI010000420 DADLTI010000000
VERSION     DADLTI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.427"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..568)
                     /locus_tag="JN755_004471"
     CDS             complement(<1..568)
                     /locus_tag="JN755_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7382975.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVE"
BASE COUNT          163 a          160 c          152 g          165 t
ORIGIN      
        1 tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac agcacattca
       61 gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat gcgatagatt
      121 ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat ccggcctgaa
      181 tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa tgccgtatcc cgggttctgc
      241 atgtcgcaac tgtattaagg tattgctctg cggcatcact cagggctgga gtgtgttccc
      301 gacctactga ttcgcacagg taatcaacga agcctgatac cagttttcgt tcgcgaggcg
      361 tgatcgcccc accgaccgga gtccagtaat caaatcccag ttgcaggagt ttgaaaaaac
      421 gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac tcacctattt
      481 tgatttgatg caaaaaatcg caactctccg gcgtcgccgg gagaagtaat ccggaagagg
      541 tttgtttgac cagttgtata tgcgccatcg tagttctccg ctggcgcagt agaatgggtg
      601 ttcagcccgt tatgtagtat accagaatta atgccaatac
//
LOCUS       DADLTI010000421          631 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLTI010000421 DADLTI010000000
VERSION     DADLTI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.428"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(46..249)
                     /locus_tag="JN755_004472"
     CDS             complement(46..249)
                     /locus_tag="JN755_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY7382976.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            complement(308..>631)
                     /locus_tag="JN755_004473"
     CDS             complement(308..>631)
                     /locus_tag="JN755_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY7382977.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
BASE COUNT          156 a          150 c          140 g          185 t
ORIGIN      
        1 ttggaggtaa tttttcgtgt tcaggatttt tccttcgtct tcccatcaga tcgcaccaaa
       61 attcggctct acctcgcgtg gtggtaaagt tttattgcag gtaaatagat cccggctgac
      121 aatcggtttg ccactacgat tggtatagaa cggaagcccg ttttccatta accattttcg
      181 ctggtggctt gcatatttgc agcccgttaa tattagcaat tcatcttcgg ttaaaaataa
      241 gctgctcata gctatatctc ataaccgccg ctaactatat acggttagcg gcaattaggg
      301 ttgaacatta aaaatcagcc tgactcggga tcagtttttg ccagatagct gaaacgtatt
      361 ttgcctggta acgagcgtca tcaagtgcat tatggcgctc accttcgaat ggaatagccg
      421 ttctggcatc gaagtctatg gctttcccca gctcaacgat tgtgcgtaca tcgcgatcgt
      481 tgtagtaacg ccacgggcag gggatcccct gccgttcgta tgaacggcgc aaaatcgtgt
      541 tgtcgaagtt ggctccattt ccccagacct gaacaaaaaa ttcaccggag ttttcgtcga
      601 taaattcccg caattgtaac agtgcatcat c
//
LOCUS       DADLTI010000422          613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLTI010000422 DADLTI010000000
VERSION     DADLTI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.429"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JN755_004474"
     CDS             complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JN755_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY7382978.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          185 a          122 c           91 g          215 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aactctggcg actcatctct taacacttgc
      181 ataatctctc tttttatatc atatagttct ataaaccgat ttgcccgtag ttctccttgg
      241 ggagaatcat ttctgaatac aacaattata tacaaaaaat cactattttc tttagagaag
      301 atcttacaac taatctctgg gagctcacat aatgcatgta agtcaggatg gtacaaacta
      361 tccacagaaa attgccccat aggacgtttt ccagatataa ctgtcaacca gcttccttcc
      421 ggtgtactgg cttcgtctct ggaaataggc tgaaaatctt ccggacatgg agaatacatt
      481 gtcttcatat aattaagtcg tctggatttc tcattaataa actcctctat ttttaattgt
      541 gccatacttc tggttatacc agagatatac ttcttgtcat atatgtttgg tgattcatta
      601 aagtaaccat gtt
//
LOCUS       DADLTI010000423          594 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLTI010000423 DADLTI010000000
VERSION     DADLTI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.430"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            57..554
                     /locus_tag="JN755_004475"
                     /pseudo
     CDS             57..554
                     /locus_tag="JN755_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          144 a          146 c          183 g          121 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catc
//
LOCUS       DADLTI010000424          568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLTI010000424 DADLTI010000000
VERSION     DADLTI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.431"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            31..318
                     /locus_tag="JN755_004476"
     CDS             31..318
                     /locus_tag="JN755_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY7382979.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            378..>568
                     /locus_tag="JN755_004477"
     CDS             378..>568
                     /locus_tag="JN755_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382980.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDIT"
BASE COUNT          175 a          117 c          146 g          130 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattac
//
LOCUS       DADLTI010000425          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLTI010000425 DADLTI010000000
VERSION     DADLTI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.432"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..297
                     /locus_tag="JN755_004478"
     CDS             <1..297
                     /locus_tag="JN755_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7382981.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JN755_004479"
     CDS             complement(405..>566)
                     /locus_tag="JN755_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382982.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLTI010000426          558 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLTI010000426 DADLTI010000000
VERSION     DADLTI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.433"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            14..>558
                     /locus_tag="JN755_004480"
     CDS             14..>558
                     /locus_tag="JN755_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382983.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYA"
BASE COUNT          120 a          151 c          159 g          128 t
ORIGIN      
        1 ccccgccctt ctgatgcttc cccgttttgc cgatattttt cagcagggaa accgctggct
       61 taactggctg gagaaacaac cggaaggggc agtccgtccg gtggtaattg aatcagtgac
      121 aaaaatcatg gcctgcggga ccacactgat gggctacacg cagtggtgtt gttcatcacc
      181 ggactgttgc catacaaaaa aggtctgctt ccggtgtaaa agccgctcct gcccgcactg
      241 cggggtgaag gctggcgcac agtggataca gtatctgctg agcctggtcc ccgactgccc
      301 gtggcagcat attgtgttca cacttccctg ccagtactgg tccctggtgt tccacaaccg
      361 gtggttactg gcagagatga gccgcattgc agcggatgtg atactggaaa tctgccatca
      421 ggcagatgtg gagccgggga tattcacggt gatccacaca tgggggcgtg accagcagtg
      481 gcatccgcat atccatttat cgacaactgc cggtggtgtg acgtcgggcc acacctggaa
      541 aaatcttcat ttttacgc
//
LOCUS       DADLTI010000427          555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLTI010000427 DADLTI010000000
VERSION     DADLTI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.434"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>555
                     /locus_tag="JN755_004481"
     CDS             <1..>555
                     /locus_tag="JN755_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY7382984.1"
                     /translation="VNMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNEAFNRELAKLN
                     QSLRETLSKFNKTTSKNNYSENASNSLDVLATSAIKAGTAIYGFQTALEAYRKVMEIG
                     LKKEASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETFSSFAKFSAGAGDMNADQ
                     KESLFSNVIGTSRLMGLSTDEING"
BASE COUNT          187 a           87 c          121 g          160 t
ORIGIN      
        1 gagtgaatat gagcaagctt cgtgacttta atcctatgct tgagaagtat tttaaaagcc
       61 agtattacag cttaagcagt aaatccaaga cacttggaaa cgaagctttt aatcgtgagt
      121 tagcaaaatt aaatcaatct cttcgtgaaa cactttctaa gtttaataag accaccagca
      181 aaaataatta cagtgaaaat gcttcaaata gtctggatgt attagctaca agtgcaatca
      241 aagcaggtac agccatttat ggttttcaga cagccttaga agcttatagg aaggttatgg
      301 agattggtct gaaaaaagaa gcatcacaac gagcggcaca attcgtttta ggtgatgaag
      361 gtgctaaacg agctacagaa tttgttaaga atcttgctga tagtagtggt gtagaccaga
      421 ttgaaacatt tagcagtttt gctaagtttt ccgctggtgc tggtgatatg aatgcagacc
      481 agaaagaatc cttattttct aatgtgattg gtacatcccg actaatgggt ttaagcactg
      541 atgagattaa cggga
//
LOCUS       DADLTI010000428          533 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLTI010000428 DADLTI010000000
VERSION     DADLTI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.435"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            5..>533
                     /gene="fimC"
                     /locus_tag="JN755_004482"
     CDS             5..>533
                     /gene="fimC"
                     /locus_tag="JN755_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7382985.1"
                     /translation="MTFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQVQLA
                     VTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDR
                     ESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSA
                     NSLTLINPTPYYLTVT"
BASE COUNT          162 a          113 c          126 g          132 t
ORIGIN      
        1 ggaaataaca ttctgcttgc tggcaggtat cctgatgttc atggcaatga tggttgccgg
       61 acgcgctgaa gcaggagtgg ccttaggtgc gactcgcgta atttatccgg cagggcaaaa
      121 acaagtgcaa cttgccgtga caaataatga tgaaaatagt acctatttaa ttcaatcatg
      181 ggaggaaaat gccgatggtg taaaggatgg tcgttttatc gtgacgcctc ctctgtttgc
      241 gatgaaggga aaaaaagaga ataccttacg tattcttgat gcaacaaata accaattgcc
      301 acaggaccgg gaaagtttat tctggatgaa cgttaaagcg attccgtcaa tggataaatc
      361 aaaattgact gagaatacgc tacagctcgc aattatcagc cgcattaaac tgtactatcg
      421 cccggctaaa ttagcgttgc cacccgatca ggccgcagaa aaattaagat ttcgtcgtag
      481 cgcgaattct ctgacgctga ttaacccgac accctattac ctgacggtaa cag
//
LOCUS       DADLTI010000429          532 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLTI010000429 DADLTI010000000
VERSION     DADLTI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.436"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>532
                     /locus_tag="JN755_004483"
     CDS             <1..>532
                     /locus_tag="JN755_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY7382986.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGL
                     PPNESENRYWKNSNSVA"
BASE COUNT          136 a          129 c          161 g          106 t
ORIGIN      
        1 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
       61 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      121 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      181 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      241 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      301 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
      361 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
      421 taacggacta tatcgttgga tattacagcg cactaagacc gcacgaatat aacggtgggt
      481 tacccccaaa cgaatcggaa aatcgatact ggaaaaactc taactcggtg gc
//
LOCUS       DADLTI010000430          530 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLTI010000430 DADLTI010000000
VERSION     DADLTI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.437"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>530
                     /locus_tag="JN755_004484"
     CDS             <1..>530
                     /locus_tag="JN755_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7382987.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KIHDEDVRRPTEMTQEA"
BASE COUNT          136 a          130 c          161 g          103 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaatccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc
//
LOCUS       DADLTI010000431          514 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLTI010000431 DADLTI010000000
VERSION     DADLTI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.438"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            28..489
                     /locus_tag="JN755_004485"
     CDS             28..489
                     /locus_tag="JN755_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY7382988.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          162 a          104 c          114 g          134 t
ORIGIN      
        1 tcttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt ttccctcgtt
       61 gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcgaagaaa
      121 ttcagcgcca atctgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag
      181 gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa
      241 gaagatgctg ccaagacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt
      301 gaagaaaaat caacctatac cgatacctac gcgcaggaaa acgtgactat cgatatggaa
      361 aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc
      421 aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa
      481 gtgaaataat ctgtcggcgg ccatgtttcg catg
//
LOCUS       DADLTI010000432          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLTI010000432 DADLTI010000000
VERSION     DADLTI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.439"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>512
                     /locus_tag="JN755_004486"
     CDS             <1..>512
                     /locus_tag="JN755_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7382989.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPE"
BASE COUNT          133 a          138 c          127 g          114 t
ORIGIN      
        1 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
       61 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
      121 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
      181 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
      241 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
      301 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
      361 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
      421 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
      481 tccgacgctc gtgatgcgtt cagtcctgag ca
//
LOCUS       DADLTI010000433          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLTI010000433 DADLTI010000000
VERSION     DADLTI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.440"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            118..>509
                     /locus_tag="JN755_004487"
     CDS             118..>509
                     /locus_tag="JN755_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7382990.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAG"
BASE COUNT          150 a          120 c          137 g          102 t
ORIGIN      
        1 aaatgaagct cgccttgagc gagatatatt aaaaaaagca acagcgtatt ttgcacagga
       61 gtcgctgaaa aatacgcgtt aatcgaacaa tggcgacaac aatttcccat tgaagcgatg
      121 tgtcaggtat ttggtgtatc caggagcggt tattacaact gggtacagca tgaaccctca
      181 gacagaaaac aaagtgatga gcggctaaaa ctggagatta aggtggcaca tatccgcact
      241 cgcgaaacat atggaacccg gcggctccag acggagctgg cagagaatgg catcatcgtt
      301 ggtcgtgacc gactggcacg tcttcgtaag gagctgaggc tacgctgtaa gcagaaacgc
      361 aagttcagag cgactacgaa cccgaaccac aatctgccag ttgcgccaaa tctgctgaac
      421 cagacgttcg ctcctacagc accaaatcag gtctgggtgg cggacctgac gtatgttgcc
      481 acacaggagg gatggttgta cctcgctgg
//
LOCUS       DADLTI010000434          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLTI010000434 DADLTI010000000
VERSION     DADLTI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.441"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            46..>509
                     /locus_tag="JN755_004488"
     CDS             46..>509
                     /locus_tag="JN755_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382991.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFL"
BASE COUNT          143 a          107 c          128 g          131 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttga
//
LOCUS       DADLTI010000435          508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLTI010000435 DADLTI010000000
VERSION     DADLTI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.442"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..208
                     /locus_tag="JN755_004489"
     CDS             <1..208
                     /locus_tag="JN755_004489"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382992.1"
                     /translation="VAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENARLKR
                     QLAEQAEELAILQKAATYFAKRLK"
     gene            205..>508
                     /locus_tag="JN755_004490"
     CDS             205..>508
                     /locus_tag="JN755_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7382993.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          117 a          143 c          152 g           96 t
ORIGIN      
        1 tgttgctgcc gcagcccgtg aactcagcct gtatgaatct cagctctatg cgtggcgcag
       61 caaactgcag caacagatga cctcgtctga gcgtgaaagt gaactggccg ctgaaaatgc
      121 ccgcctgaag cggcaactgg cggagcaggc agaggaactg gctatcctcc aaaaggccgc
      181 gacatacttt gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgag
      241 ttcagcatca aggcaatgtg ccgcgtgctc cgggtggccc gcagcggctg gtatacgtgg
      301 tgtcagcggc ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
      361 gtcctcgctg cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat
      421 gaactgcgtg ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt
      481 caggggctga gggcaaaggc ctcccgga
//
LOCUS       DADLTI010000436          493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLTI010000436 DADLTI010000000
VERSION     DADLTI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.443"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..394
                     /locus_tag="JN755_004491"
     CDS             <1..394
                     /locus_tag="JN755_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7382994.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
                     LLERLGHIPPAEAEKAYYASIGNDDLAA"
BASE COUNT          145 a          120 c          136 g           92 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgcagaag tggaactggc cacactaacg tgggtggact ggtataacaa
      301 tcgacgattg ctggaaaggc tgggccacat ccctccggca gaagcagaaa aagcttatta
      361 tgcttccatc ggaaacgatg atctggcagc ctgagttcac agataaaaca ctctccagga
      421 aacccggggc ggttcacaga agtatgccgc agacctgcaa aatcagcagt ggcagacgat
      481 catgaaaaac ctt
//
LOCUS       DADLTI010000437          491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLTI010000437 DADLTI010000000
VERSION     DADLTI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.444"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..>491)
                     /gene="intI1"
                     /locus_tag="JN755_004492"
     CDS             complement(<1..>491)
                     /gene="intI1"
                     /locus_tag="JN755_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7382995.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWL"
BASE COUNT          112 a          166 c          135 g           78 t
ORIGIN      
        1 ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca ggctgggtgc caagctctcg
       61 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
      121 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
      181 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
      241 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
      301 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
      361 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
      421 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
      481 acccagtgga c
//
LOCUS       DADLTI010000438          490 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLTI010000438 DADLTI010000000
VERSION     DADLTI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.445"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..137
                     /locus_tag="JN755_004493"
     CDS             <1..137
                     /locus_tag="JN755_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="NAD(P)-binding; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAY7382996.1"
                     /translation="QGEIIATADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW
                     "
     gene            246..478
                     /locus_tag="JN755_004494"
                     /pseudo
     CDS             246..478
                     /locus_tag="JN755_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT          135 a           97 c          153 g          105 t
ORIGIN      
        1 cgcaaggcga gattatcgct accgccgacg cgcataaggc aacgcgtatt gatgcggagc
       61 tgtcgatggt ggcattgcgg gaatatagag aaaagtttcc ggcatggcgg gatgcggatg
      121 agtttaggtt gtggtgagtg ggggttgtgt ttagggccgg ggctgggcta gattacgaaa
      181 gcccctaact taaacacaat attacgccag aagtcaccat ttccaaaaca cagcgtcgct
      241 gtaccgtggg ttatctgccc aaccgcagcg acacgccggt gatcaaaatc agcgccaaat
      301 ggctgagaaa agcgggattt gagatcggca cgggcgttac cgtgaagatc tcggagggat
      361 gtttgatcct gcttgggata acaacgaagt acaggaactg cgcagggaac tttatcaggt
      421 taaacagtca gttaagggga tgcgagacgg gatgtttagt gtgctgaacg aaagttaaat
      481 aataaaaagc
//
LOCUS       DADLTI010000439          485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLTI010000439 DADLTI010000000
VERSION     DADLTI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.446"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            114..485
                     /locus_tag="JN755_004495"
                     /pseudo
     CDS             114..485
                     /locus_tag="JN755_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          132 a          127 c          141 g           85 t
ORIGIN      
        1 atgcacaacg ttttgcggag aaagcccgac aggatgcaac cgtcacagct gaggacagaa
       61 aggccactgc ggaagatgtg acaagcacag gagcaaatgc agccgcagcc ggacagagcg
      121 cacaggatgc cgcaggttat gcccgcgcag cagaacaggc caaaaatgac attgatgctg
      181 cgctgaccgg caccctgaaa acggctaacc atctgtctga aatcgcagca gcaggcgaaa
      241 aggcacaaca gaagtcccgg gataatctgg ggctgaaaag tgcggccacg atggaagcac
      301 agagcgacat ttacgaccgg acaaaaggcc gtctggcgat acccggcgca ttcggctttg
      361 ggtgtgcttt tctgcctgaa gatgttatcc gttttgacac taagagtgat ttcctggcct
      421 gggtaaggaa tgcgctgcca ggtgaatatt ccgttgctgg cccttacggc atcatcatac
      481 ccgac
//
LOCUS       DADLTI010000440          481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLTI010000440 DADLTI010000000
VERSION     DADLTI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.447"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..252)
                     /locus_tag="JN755_004496"
     CDS             complement(1..252)
                     /locus_tag="JN755_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7382997.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          136 a           99 c           89 g          157 t
ORIGIN      
        1 ctaacaggat gctctgactc gcaattcatc cagcagttta tcatttccca taatgtcact
       61 taccctcatc ggtaaaaaaa ttgcctttcg accattacga tacattattg attttggggt
      121 ttcagggaag taatccattt cgactataac tgacaggtca tcacgacgta tgactgcgta
      181 tttgctacta aatagtttct ttattttctc cacgatgcct ccaggtttat aagtacaaac
      241 ggttatatcc acatagagac aaaaatatta atctgaaaaa tatttatttc acgccgtata
      301 tttgattgtt taatgtgcaa gtacaatgac ttttattttt tgttgtgtat ataatcaaat
      361 atatggttat ttttcaccct gcgtattcag cacgcaacaa aaaacccgcc gaagcgggtt
      421 tagtgcgggt gcgttgagga tgcctgacac atgagaggtg gcgagggatt tctcccccgc
      481 c
//
LOCUS       DADLTI010000441          474 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLTI010000441 DADLTI010000000
VERSION     DADLTI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.448"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..411)
                     /locus_tag="JN755_004497"
     CDS             complement(<1..411)
                     /locus_tag="JN755_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY7382998.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHD"
BASE COUNT          111 a          117 c           94 g          152 t
ORIGIN      
        1 atcatgttcg aacaccactc caacatctgt ttgaacacct gttaaatttc tctcttgttt
       61 tgtttgaaca tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc
      121 tgattttttg gttaattttt ctctgacaga tgccagatct tcctcaatcc gaagatgcat
      181 ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat ggttcaacat caacccatcg
      241 ctcgttagtc agacgggcaa tttttgccaa cctgtttttg ggtattggct ttcctgtttg
      301 ccagtaattg aacatcagca acaaatacgc gccgtgctcc tctgctgaca aatgcatggt
      361 gtcagccaga taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc
      421 gtatgatgct acccggttgc ttagaataag tgaggtagcc tgagtttaac ggac
//
LOCUS       DADLTI010000442          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLTI010000442 DADLTI010000000
VERSION     DADLTI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.449"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="JN755_004498"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="JN755_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7382999.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(337..>473)
                     /locus_tag="JN755_004499"
     CDS             complement(337..>473)
                     /locus_tag="JN755_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7383000.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT          120 a          110 c          115 g          128 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt
      301 ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca tataggtctg
      361 attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc tgctgttgta
      421 gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga act
//
LOCUS       DADLTI010000443          468 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLTI010000443 DADLTI010000000
VERSION     DADLTI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.450"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>468
                     /locus_tag="JN755_004500"
     CDS             <1..>468
                     /locus_tag="JN755_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7383001.1"
                     /translation="LTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIE
                     EIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLT
                     ETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAE"
BASE COUNT          130 a          133 c          112 g           93 t
ORIGIN      
        1 acctcaccga tcgcggcgaa ggtttcgtcc acgatctgac gtcactgacg cgcgatgtag
       61 ccactggcgt actggcccgt tcaatggacg tggacatcta taaccttcat ccggcacacg
      121 ctaaacgcat tgaggaaatt atcgctgaaa ataaaccacc cttttctgtt ttccgcgaca
      181 aattcatcac catgcctggc gggctggatt attcccgcgc catcgtggtt gcgtccgtga
      241 aagaagcacc aattgggatc gaggtcatcc ccgcacacgt cactgaatat ctgaacaaag
      301 tactgactga aaccgatcat gccaaccctg atccggaaat cgtggatatt gcctgcggtc
      361 gctcctctgc cccgatgccg cagcgagtaa cagaagaagg aaaacaggac gatgaagaaa
      421 aaccacaacc atctggcgca atgacagatg aacaggcaac ggctgaaa
//
LOCUS       DADLTI010000444          467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLTI010000444 DADLTI010000000
VERSION     DADLTI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.451"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..456
                     /locus_tag="JN755_004501"
     CDS             <1..456
                     /locus_tag="JN755_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7383002.1"
                     /translation="YDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKH
                     AEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGES
                     AAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          133 a           99 c          140 g           95 t
ORIGIN      
        1 tacgacatag aagcggagat acgtggcagt ccggcagagg aacggcttgc agtcagaaaa
       61 gccagaagcg tccagttgat gcagtcgttg tacgactgga tacagttgca gaggaaaacg
      121 ctgtcgaaac atgcggagat ggcgaaggcg ttcgactata tcctgaatca ctggaatgcg
      181 ctgaacgagt tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg
      241 ttacgatcgg tggcggttgg aagaaaaaat tatctctttt tcggctcaga caagggagga
      301 gaaagtgcgg cgatcatcta cagtctgctg gtcacctgca aacagaacga agtggagccg
      361 gaggactggt tgcgcgaagt gatcgagaag ctcaatgact ggccgtcgaa ccaagtgcat
      421 gaactgctgc cctggaactt ctcgtctgta aaataatcct tacgcta
//
LOCUS       DADLTI010000445          463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLTI010000445 DADLTI010000000
VERSION     DADLTI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.452"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..463
                     /locus_tag="JN755_004502"
                     /pseudo
     CDS             1..463
                     /locus_tag="JN755_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          126 c          121 g           92 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg tta
//
LOCUS       DADLTI010000446          463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLTI010000446 DADLTI010000000
VERSION     DADLTI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.453"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..184
                     /locus_tag="JN755_004503"
     CDS             <1..184
                     /locus_tag="JN755_004503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383003.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            279..>463
                     /locus_tag="JN755_004504"
     CDS             279..>463
                     /locus_tag="JN755_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7383004.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          138 a           91 c          136 g           98 t
ORIGIN      
        1 acaactcgaa aaaacgggca taacaaaaga tgctatccgt aaagatatgg tacccgcgct
       61 tcgggaactg atgcaggcga agcatcgcgc acagaaagca gtagatgagc tggtggactg
      121 tgtggcagag ctggaaacca gagtcggaaa gtttgaaaaa ctggtgcagg aggcgctgcg
      181 ctgatgcgcc atgagtttgt tttgccctgg cttgtgcgcg atcattatgt ccctggtgga
      241 aagcccggga taaaaatcac cgaaccggag agtatttgat gagcgtcaaa attcaaacga
      301 taccggaact gcttatccag acacgaggta atatgaccga agtttcacga atgctgaact
      361 gtaatcgcgc cacggtaaga aaatatgctg aagataaaga gggcaaaggg cacgccattg
      421 ttgatggtgt tctgattgtt catcgtggat gggacagggg taa
//
LOCUS       DADLTI010000447          449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLTI010000447 DADLTI010000000
VERSION     DADLTI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.454"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(48..266)
                     /locus_tag="JN755_004505"
     CDS             complement(48..266)
                     /locus_tag="JN755_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383005.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           96 a          108 c          126 g          119 t
ORIGIN      
        1 agcactggtt ggctgtgaac aggcgtttca gcgggcgggg ctgaaaatca ccaccacttc
       61 gggtcatccg ccttcatctc cgcttctgtt tcgtataaat caaaacgacg gcaggtatgg
      121 cagaacgtga cgtattcgta cggtgaatcc gggtacattt tgtggaaggt ttcccagaaa
      181 cagaccgtgc aggagggatg accggtgata aagtctgcaa atacggactt ataagggtga
      241 ttattggctc tggcgacggc tttcagaacc ctctttcacc attctggtgt ggactttctg
      301 gtgctccagg ttgtgtgaca tgggaactca ttctggatgg ttactctgaa agctcatatt
      361 ctgccacacc ccgatttgca gccgccaggc tgccgtggtt cttacgccag aagggatttg
      421 taactggtct ggtcggctcc ggcgtatca
//
LOCUS       DADLTI010000448          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLTI010000448 DADLTI010000000
VERSION     DADLTI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.455"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            211..>442
                     /locus_tag="JN755_004506"
     CDS             211..>442
                     /locus_tag="JN755_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7383006.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          106 c           63 g          134 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccctggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg ag
//
LOCUS       DADLTI010000449          436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLTI010000449 DADLTI010000000
VERSION     DADLTI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.456"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>436
                     /locus_tag="JN755_004507"
     CDS             <1..>436
                     /locus_tag="JN755_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7383007.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY"
BASE COUNT           99 a          139 c          116 g           82 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttatt
//
LOCUS       DADLTI010000450          429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLTI010000450 DADLTI010000000
VERSION     DADLTI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.457"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            108..>429
                     /locus_tag="JN755_004508"
     CDS             108..>429
                     /locus_tag="JN755_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383008.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPCGVNIRGVGKASQLRAKSGLTGS
                     VLRLS"
BASE COUNT          121 a           87 c          105 g          116 t
ORIGIN      
        1 attttccgaa tttgctgaag tatgaccctg atcagttcgg tccggattta attgagcaac
       61 tagctcaatc cggtaagtat tcgcaggata acaccaaagg cgatgccatg attggcgtca
      121 agcagccttt accaaaagca gttttaagaa ctcagcatga caaaaataaa gaagcaataa
      181 gtatcctgga ttttggtgtt attgatgatg gtgtgacaga taattaccag gcaatacaaa
      241 atgcaataga tgccgttgct tcactaccct ccggcgggga gctgtttatc cctgcgagca
      301 accaagcggt ggggtatatt gttggatcca ctttgcttat tccttgcggt gttaacatca
      361 gaggggttgg taaggcatcg caactccgag caaaaagcgg acttacagga tctgtgttaa
      421 ggctgtctt
//
LOCUS       DADLTI010000451          426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLTI010000451 DADLTI010000000
VERSION     DADLTI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.458"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..426
                     /locus_tag="JN755_004509"
                     /pseudo
     CDS             1..426
                     /locus_tag="JN755_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DADLTI010000452          419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLTI010000452 DADLTI010000000
VERSION     DADLTI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.459"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>419
                     /locus_tag="JN755_004510"
     CDS             <1..>419
                     /locus_tag="JN755_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7383009.1"
                     /translation="GPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTI
                     GVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWD
                     FRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQEL"
BASE COUNT           82 a          123 c          123 g           91 t
ORIGIN      
        1 ggtccggtgc aaatctgctg gcataaattc gcccgctact gggatgtgga gctgcgtgag
       61 atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga agcctgcgac
      121 gaaaatacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa ctatgagttc
      181 ccgcaaccgc tgcacgatgc actggataaa ttccaggccg acaccggtat cgacatcgac
      241 atgcacatcg acgccgccag cggtggcttt ctggcaccgt tcgtcgcccc ggatatcgtc
      301 tgggacttcc gcctgccgcg tgtgaaatcg atcagtgctt caggccataa attcggtctg
      361 gctccgctgg gctgcggctg ggttatctgg cgtgacgaag aagcgctgcc gcaggaact
//
LOCUS       DADLTI010000453          396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLTI010000453 DADLTI010000000
VERSION     DADLTI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.460"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>396
                     /locus_tag="JN755_004511"
     CDS             <1..>396
                     /locus_tag="JN755_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7383010.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDP"
BASE COUNT          114 a           98 c          108 g           76 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctc
//
LOCUS       DADLTI010000454          382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.461, whole genome shotgun sequence.
ACCESSION   DADLTI010000454 DADLTI010000000
VERSION     DADLTI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.461"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            1..361
                     /locus_tag="JN755_004512"
                     /pseudo
     CDS             1..361
                     /locus_tag="JN755_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
BASE COUNT          108 a           93 c           94 g           87 t
ORIGIN      
        1 attacgcaag gctgttgcag ttgctattgg tggtggggct attgccatag cgtctgtgct
       61 catcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
      121 tatcgttggc gtatggactg tatgccacgg acacaccgga aaagacatca tgctcggtaa
      181 aacgtatacc gaagcagaat gcaaagccct cctgaataaa gaccttgcca ctgtcgccag
      241 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      301 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg atgcttcttc gcaaaataaa
      361 ctagggcgat atcaaaggcg ca
//
LOCUS       DADLTI010000455          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.462, whole genome shotgun sequence.
ACCESSION   DADLTI010000455 DADLTI010000000
VERSION     DADLTI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.462"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>375
                     /locus_tag="JN755_004513"
     CDS             <1..>375
                     /locus_tag="JN755_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7383011.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLTI010000456          363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.463, whole genome shotgun sequence.
ACCESSION   DADLTI010000456 DADLTI010000000
VERSION     DADLTI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.463"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..167
                     /locus_tag="JN755_004514"
     CDS             <1..167
                     /locus_tag="JN755_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HAY7383012.1"
                     /translation="FGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIESKHRP
                     KYIIKNEKQK"
BASE COUNT          113 a           67 c           69 g          114 t
ORIGIN      
        1 tctttggcaa cccagttcgc ggatatccct ccctgcttgt atcaatactt ttcttgggtg
       61 gagtgcagct gatcgggatt ggtgttctcg gtgaatatat aggcagaatc tatattgaaa
      121 gcaaacatcg tcccaaatac atcatcaaaa atgagaaaca aaaatgaaaa gccttaaaac
      181 atcatatgta aaaaaactat ttttcttaat tgctgtattt ttcattgcgg ttattttgat
      241 gtatttgcgc cgccctgata taatcagaac accgcagttt tgggcagaag atggtcatgt
      301 atggtatgct atggcataca ataatggcat attcacatca atgatattcc ctcaaaatgg
      361 ata
//
LOCUS       DADLTI010000457          352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.464, whole genome shotgun sequence.
ACCESSION   DADLTI010000457 DADLTI010000000
VERSION     DADLTI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.464"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
BASE COUNT          107 a           92 c           69 g           84 t
ORIGIN      
        1 ttccctcgcg atattaacgt aatatcttaa ttgtatgcac cgccagaatg cgttcagtgc
       61 gatcacggca gcagacaggt aaaaatagca acaaaccacc cgaaaaaccg ccgcgatcgc
      121 gtatgataaa ttttaaccac atgcatacct gtgcagcaag gcgaatcacg cacggatttc
      181 cagccagaga cacctgaaac gtaaccggct catttaaacc gtctggtctg ttacctccgg
      241 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
      301 gaatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gt
//
LOCUS       DADLTI010000458          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.465, whole genome shotgun sequence.
ACCESSION   DADLTI010000458 DADLTI010000000
VERSION     DADLTI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.465"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..208)
                     /locus_tag="JN755_004515"
     CDS             complement(<1..208)
                     /locus_tag="JN755_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7383013.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JN755_004516"
     CDS             complement(205..>351)
                     /locus_tag="JN755_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7383014.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADLTI010000459          342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.466, whole genome shotgun sequence.
ACCESSION   DADLTI010000459 DADLTI010000000
VERSION     DADLTI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.466"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>342
                     /locus_tag="JN755_004517"
     CDS             <1..>342
                     /locus_tag="JN755_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7383015.1"
                     /translation="WEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
                     YQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKL
                     PTRRGRAFPRV"
BASE COUNT          100 a           60 c          110 g           72 t
ORIGIN      
        1 gctgggaaat cgaactggga tacagggaga taaaacagac gatgcaactg agcaggctga
       61 cgctgagaag taaaaagccg gagcttgtgg agcaagagct gtggggtgtc ttactggctt
      121 ataatctggt gagatatcag atgattaaaa tggcggaaca tctgaaaggt tactggccga
      181 atcaactgag tttctcagaa tcatgcggaa tggtgatgag aatgctgatg acattgcagg
      241 gcgcttcacc gggacgtata ccggagctga tgcgcgatct tgcaagtatg ggacaacttg
      301 tgaaattacc gacgagaagg ggaagggcct tcccgagagt gg
//
LOCUS       DADLTI010000460          336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.467, whole genome shotgun sequence.
ACCESSION   DADLTI010000460 DADLTI010000000
VERSION     DADLTI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.467"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..>336
                     /locus_tag="JN755_004518"
     CDS             <1..>336
                     /locus_tag="JN755_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7383016.1"
                     /translation="IGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSA
                     DYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYI
                     ECDYNRWRRH"
BASE COUNT           83 a           76 c          101 g           76 t
ORIGIN      
        1 attggctggt caatgtcgcc acgcatgacg gcgcaactgg cctgcgatgc cctgcagatg
       61 gcgctgtggc ggcgtaagag gccccggaac gttatcgttc acacggaccg tggaggccag
      121 tactgttcag cagattatca ggcgcaactg aaacggcata atctgcgtgg aagtatgagc
      181 gcaaaaggtt gctgctacga taatgcctgc gtggaaagct tctttcattc gctgaaagtg
      241 gaatgtatcc atggagaaca ctttatcagc cgggaaataa tgcgggcaac ggtgtttaat
      301 tatatcgaat gtgattacaa tcggtggcgg cggcac
//
LOCUS       DADLTI010000461          329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.468, whole genome shotgun sequence.
ACCESSION   DADLTI010000461 DADLTI010000000
VERSION     DADLTI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.468"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..290
                     /locus_tag="JN755_004519"
     CDS             <1..290
                     /locus_tag="JN755_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7383017.1"
                     /translation="EDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTLVTRLSEM
                     NVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD"
BASE COUNT          102 a           74 c           80 g           73 t
ORIGIN      
        1 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
       61 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
      121 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
      181 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
      241 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
      301 aagaatccac ccttcaacag cttcacgaa
//
LOCUS       DADLTI010000462          326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.469, whole genome shotgun sequence.
ACCESSION   DADLTI010000462 DADLTI010000000
VERSION     DADLTI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.469"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(1..326)
                     /locus_tag="JN755_004520"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="JN755_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           94 a           80 c           53 g           99 t
ORIGIN      
        1 ggctgtacgc ttccccatgt gatttgcctc gccacttcac cgggctcagg accccgtagg
       61 atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg tagacctgtt
      121 actaatttat acctactctg accaagtctg gataagaaag gcacacctgc acatttctac
      181 ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata ttcctcgtac
      241 agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt catatattac
      301 tcgaccttgt tgagatacaa caacac
//
LOCUS       DADLTI010000463          308 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.470, whole genome shotgun sequence.
ACCESSION   DADLTI010000463 DADLTI010000000
VERSION     DADLTI010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.470"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
BASE COUNT           92 a           71 c           68 g           77 t
ORIGIN      
        1 cgcccgaagg ttagtatttc tgcacagcaa ttttgcaaaa aaaggcgatc attcataact
       61 taaacgtctt tcagtcactc cgggatttcc catcatcgca gactgaaaga ctctgactgg
      121 agcgggcagc gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa
      181 gacaggtgaa tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac
      241 ttgaagcacg taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt
      301 ttggtctg
//
LOCUS       DADLTI010000464          295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.472, whole genome shotgun sequence.
ACCESSION   DADLTI010000464 DADLTI010000000
VERSION     DADLTI010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.472"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..>295)
                     /locus_tag="JN755_004521"
     CDS             complement(<1..>295)
                     /locus_tag="JN755_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7383018.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVR"
BASE COUNT           70 a           93 c           55 g           77 t
ORIGIN      
        1 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
       61 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
      121 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
      181 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
      241 gtggcttttt caggtaagag ccaaagtaca ccgccacatg ggtggcatta tccat
//
LOCUS       DADLTI010000465          292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.473, whole genome shotgun sequence.
ACCESSION   DADLTI010000465 DADLTI010000000
VERSION     DADLTI010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.473"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            43..>292
                     /locus_tag="JN755_004522"
     CDS             43..>292
                     /locus_tag="JN755_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383019.1"
                     /translation="MRQRGIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSF
                     VDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARRRNR"
BASE COUNT           80 a           69 c           74 g           69 t
ORIGIN      
        1 acctacacag caagcgaact ggctattggt ctggtaatgc ttatgcggca acggggaatt
       61 gaagctggta accgcccggt aatgtttctg gacacagaaa caggctctga ctgggttaaa
      121 ccccgctttg atgcggaaaa tatcgaatta tttacggcaa aaacccgctc gtttgttgat
      181 ctgcttgaag ccattaatga agcggaaagc agcggttccg taatgatcat cgactccatc
      241 agtcacttct ggaccggatt atgcgacgag tatgccagac gccgaaaccg ta
//
LOCUS       DADLTI010000466          290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.474, whole genome shotgun sequence.
ACCESSION   DADLTI010000466 DADLTI010000000
VERSION     DADLTI010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.474"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            50..>290
                     /locus_tag="JN755_004523"
     CDS             50..>290
                     /locus_tag="JN755_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004968843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383020.1"
                     /translation="MCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQM
                     EKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFK"
BASE COUNT           95 a           61 c           70 g           64 t
ORIGIN      
        1 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
       61 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac
      121 cggtataaaa atgaaagcag aaacagaaac cggttatgaa ccctccatcc tgatccagat
      181 ggagaagcaa atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga
      241 tcgctccacc cgtattgatg gacaggtatt ttcaaaccca acatttaaaa
//
LOCUS       DADLTI010000467          290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.475, whole genome shotgun sequence.
ACCESSION   DADLTI010000467 DADLTI010000000
VERSION     DADLTI010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.475"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..268
                     /locus_tag="JN755_004524"
     CDS             <1..268
                     /locus_tag="JN755_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7383021.1"
                     /translation="EIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFA
                     GDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT           88 a           69 c           72 g           61 t
ORIGIN      
        1 tgaaataggc tatctgccgt tcagtcagga agaagcaaaa ctgttcttcc aggtcatcgc
       61 taaacgttac gaaaagagcg caatgatcct gacatccaat ctaccgttcg ggcagtggga
      121 tcaaacgttc gccggtgatg cagcactgac ctcagcgatg ctgggccgta tcttacacca
      181 ctcacatgtc gttcaaatca aaggagaaag ctatcgactc agacagaaac gaaaggccgg
      241 ggttatagca gaagctaatc ctgagtaaaa cggtggatca atattgggcc
//
LOCUS       DADLTI010000468          278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.476, whole genome shotgun sequence.
ACCESSION   DADLTI010000468 DADLTI010000000
VERSION     DADLTI010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.476"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            2..>278
                     /gene="tnpB"
                     /locus_tag="JN755_004525"
     CDS             2..>278
                     /gene="tnpB"
                     /locus_tag="JN755_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7383022.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT           60 a           74 c           86 g           58 t
ORIGIN      
        1 gatgataacg ctgccgaccg gtaccaaaat ctggatcatc gctggcatca cagatatgcg
       61 ttgtggcttc aatggcctgg cttcgaaggt gcagaacacg ctgaaagatg acccgttctc
      121 cgggcatatc ttcgtcttcc ggggccgcag tggcaaaatg gtgaaaatac tgtgggccga
      181 tcgtgacggg ttatgcctgt tcgccaaacg cctggaacgg ggccgcttcg tctggccggt
      241 gacccgggaa gggaaagtgc acctgacgcc agctcagt
//
LOCUS       DADLTI010000469          277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.477, whole genome shotgun sequence.
ACCESSION   DADLTI010000469 DADLTI010000000
VERSION     DADLTI010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.477"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            89..>277
                     /locus_tag="JN755_004526"
     CDS             89..>277
                     /locus_tag="JN755_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7383023.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKK"
BASE COUNT           77 a           71 c           70 g           59 t
ORIGIN      
        1 tgtttacgtc acccataatc cgaaaaaaac agaacaaaaa gccggagtca gtcagtatcc
       61 attaacaccc ggttcgcttt atgttggcgt gtacggcggc agcccggcag atatcgcggc
      121 ggccatctgg cgtaaggctc cgccgggtat tgatatgaat ggcaacacaa cgttcactgt
      181 tgcagataag gagtacgatc cgccgtatcc tgaatacgtg atcagctggc agacactcaa
      241 acccgtcagt ctgcatgtca gtgtgacgct gaaaaaa
//
LOCUS       DADLTI010000470          271 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.478, whole genome shotgun sequence.
ACCESSION   DADLTI010000470 DADLTI010000000
VERSION     DADLTI010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.478"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
BASE COUNT           76 a           54 c           60 g           81 t
ORIGIN      
        1 gcatcgggtt ggacaattta caaaagaaag ataacccata ttgtataatg cgtttaaata
       61 tgccgtctta tataaaatga tggtcagatt aatatgcgga ttatctttct acgcaaggag
      121 tatttatctc tactcccgtc aatgattgca tctcttttct ctgctaacgg tgtcgcggcg
      181 gccattgatt tatgccaggg atatgatatc aaagcgagtt gtcacgccag caggcaaagc
      241 ctttcaggca ttacgcagga ctggagtatc g
//
LOCUS       DADLTI010000471          270 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.denovo.479, whole genome shotgun sequence.
ACCESSION   DADLTI010000471 DADLTI010000000
VERSION     DADLTI010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.denovo.479"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            complement(<1..>270)
                     /locus_tag="JN755_004527"
     CDS             complement(<1..>270)
                     /locus_tag="JN755_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7383024.1"
                     /translation="EGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNA
                     FVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT           76 a           50 c           62 g           82 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattt gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc
//
LOCUS       DADLTI010000472         9029 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.158, whole genome shotgun sequence.
ACCESSION   DADLTI010000472 DADLTI010000000
VERSION     DADLTI010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.158"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..260
                     /locus_tag="JN755_004528"
     CDS             <1..260
                     /locus_tag="JN755_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7383025.1"
                     /translation="PRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGK
                     LADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
     gene            complement(442..618)
                     /locus_tag="JN755_004529"
     CDS             complement(442..618)
                     /locus_tag="JN755_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7383026.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(847..924)
                     /locus_tag="JN755_004530"
                     /pseudo
     CDS             complement(847..924)
                     /locus_tag="JN755_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            948..1763
                     /gene="sul2"
                     /locus_tag="JN755_004531"
     CDS             948..1763
                     /gene="sul2"
                     /locus_tag="JN755_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7383027.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1824..2627
                     /locus_tag="JN755_004532"
                     /note="aph(3'')-Ib"
     CDS             1824..2627
                     /locus_tag="JN755_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7383028.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2627..3463
                     /locus_tag="JN755_004533"
     CDS             2627..3463
                     /locus_tag="JN755_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7383029.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3435..3974)
                     /locus_tag="JN755_004534"
     CDS             complement(3435..3974)
                     /locus_tag="JN755_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7383030.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(4184..5044)
                     /locus_tag="JN755_004535"
     CDS             complement(4184..5044)
                     /locus_tag="JN755_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7383031.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(5227..5784)
                     /locus_tag="JN755_004536"
     CDS             complement(5227..5784)
                     /locus_tag="JN755_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7383032.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            5948..8956
                     /locus_tag="JN755_004537"
     CDS             5948..8956
                     /locus_tag="JN755_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7383033.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
BASE COUNT         2103 a         2365 c         2403 g         2158 t
ORIGIN      
        1 taccccgaca gtacagcacg ggaggtcgga cgacattgtt agggattagc aagcggggca
       61 acaaaaagat ccgaactttg ttggttcagt gtgccagggt attcatacaa aaactggaac
      121 accagtctgg caagttggcc gactgggtca gggagttgtt gtgtcggaaa agcaactttg
      181 tcgtcacctg tgctctggca aacaagctgg ccagaatagc ctgggcactg acggcgcgac
      241 agcaaactta cgaagcataa aggcagaaat acaccagttt aaacaatcat tcatctggtt
      301 ttgcgaatac tgatattgat gatactaacg gcccaccggc ctgttgagga acctgtaaaa
      361 cggaaaggct cattgaagcc gtatattttc tggaggttca tcaggcgcgg aactcatcga
      421 ggcgcgggaa taaaatccca ttcagacgcc ggatagattc aagcaagcca acttgtcgtc
      481 aaaatcggtg ttgcaaaaac gggagtgacc atagattccg ttttctgagg cgacccccac
      541 atagagacct tcccggtcat tcactttgta gagtttgtcc ctgggcttga gattgcgcag
      601 cttggtatcg gtcagcatgt accttgtcct tcttcgtctc cgatacccac tctattgtaa
      661 acaagacatt tttatctttt atattcaatg gcttattttc ctgctaattg gtaataccat
      721 gaaaaatacc atgctcagaa aaggcttaac aatattttga aaaattgcct actgagcgct
      781 gccgcacagc tccataggcc gctttcctgg ctttgcttcc agatgtatgc tattctgctc
      841 ctgcagctaa tggagcaccg caaacaggtt actcgcctgg ggattccctt tcgacccgag
      901 catccgtatg agactcatgc tcgattatta ttattataga agcccccatg aataaatcgc
      961 tcatcatttt cggcatcgtc aacataacct cggacagttt ctccgatgga ggccggtatc
     1021 tggcgccaga cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga
     1081 tcgacctcgg tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa
     1141 tcgcgcgtat cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg
     1201 acagttatca acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg
     1261 atattcgcgg ttttccagac gctgcgttct atccgcaatt ggcgaaatca tctgccaaac
     1321 tcgtcgttat gcattcggtg caagacgggc aggcagatcg gcgcgaggca cccgctggcg
     1381 acatcatgga tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg
     1441 gtatcaaacg caaccgcctt gtccttgatc ccggcatggg gttttttctg ggggctgctc
     1501 ccgaaacctc gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc
     1561 cggtgcttct gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg
     1621 gggatgtcgg ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt ggagctgact
     1681 tcatccgcac acacgagccg cgccccttgc gcgacgggct ggcggtattg gcggcgctga
     1741 aagaaaccgc aagaattcgt taactgcaca ttcgggatat ttctctatat tcgcgcttca
     1801 tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt gaatcgcatt
     1861 ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga cgtggtgacg
     1921 ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg
     1981 accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg
     2041 aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc
     2101 tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc
     2161 acagcctatc ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg
     2221 ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga
     2281 gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc
     2341 aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg
     2401 accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc
     2461 gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg
     2521 aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg
     2581 aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc
     2641 tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag
     2701 cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc
     2761 tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag
     2821 gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga
     2881 gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc
     2941 ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc
     3001 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
     3061 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
     3121 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
     3181 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
     3241 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
     3301 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
     3361 ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc
     3421 tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc
     3481 ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta
     3541 ggattccgcc ccttcccgca aacgacccca aacaggaaac gcagctgaaa cgggaagctc
     3601 aacacccact gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt
     3661 tcggccatcc gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg
     3721 accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc
     3781 ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc
     3841 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt
     3901 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc
     3961 gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta
     4021 gcggaaagtt cttttagggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4081 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4141 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4201 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4321 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     4501 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4561 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4621 ctccgatcgt tgtcagaagt aagttggcag cagtgttatc actcatggtt atggcagcac
     4681 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4801 cacgggataa taccgcacca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacgaaaa tgttgaatac
     5041 tcatactctt cctttttcaa tattattgaa gcatttacca gggttattgt ctcatgagcg
     5101 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5161 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5221 ggcgtatcac gaggcccttt cgtcttcaag aattttataa accgtggagc gggcaatact
     5281 gagctgatga gcaatttccg ttgcaccagt gcccttctga tgaagcgtca gcacgacgtt
     5341 cctgtccacg gtacgcctgc ggccaaattt gattcctttc agctttgctt cctgtcggcc
     5401 ctcattcgtg cgttctagga tcctccggcg ttcagcctgt gccacagccg acaggatggt
     5461 gaccaccatt tgccccatat caccgtcggt actgatcccg tcatcaatga accggactgc
     5521 cacgccctga gcgtcaaatt cctttatcag ttggatcata tcggcagtgt cgcggccaag
     5581 acggtcgagc ttcttaacca gaatgacatc accttcctcc accttcatcc tcagcaaatc
     5641 cagcccttcc cggtctgttg aactgccgga tgccttatcg gtaaatatac ggtttgcttt
     5701 cacacctgcg tctttgagtg ctctgacctg aagatcaaga gactgctgac tggttgagac
     5761 ccgagcgtaa ccaaaaagtc gcataaaaat gtaccttaaa tcgaatatcg gacaactcat
     5821 gtctattatt acaaatttac gatttaatag acatattaat gtaacagttt tacgatgtcc
     5881 gataatttat aatatttcgg acggttgcga aattgttaat tcatgaccat caggcaggga
     5941 ggccggtatg cccgtcgatt ttctgaccac tgagcaggaa ctgaattatg gtcgctatgt
     6001 tgcagaaccc aatgacgtgc agctggcgcg ctattttcat cttgatgagc gggatcttgc
     6061 cttcattaac cagcggcggg gaaggcataa ccggctggga attgcgcttc agctcaccac
     6121 cgcccgtttt ctgggcacct ttctgacaga cttaacccgg gttctgccag gtgttcagca
     6181 atttgtcgcg gtgcagctta atattcgccg tccggaagtc cttacccgct acgcagaacg
     6241 ggttaccacc ctcagagagc atactgcgct gattaaggaa tattacggtt accatgaatt
     6301 tggtgatttc ccctggtctt tccgcctgaa gcgcctgctg tacacccgcg catggctcag
     6361 taatgagcga cccggcctta tgtttgattt tgccaccgcg tggctgcttc agaataaggt
     6421 cctgctgccc ggagcaacca cacttgtacg tctcgtcagt gaaatacgcg aacgggcaaa
     6481 tcagcggctg tggaagaaac tggccgcgtt gccggacagc tggcagaccg cccgcgtgac
     6541 ggagcttctg gacattcctg aggggcagcg tacttcaccg ctggagcaac tgaaaaaggg
     6601 accggtcacc gtcagcggcc cggcatttac cgaagcgctg gagcggtata tccggctgcg
     6661 aaacctggaa ttttcccgac tgaattttac cggtctgcct gctatacaac tgcgtaattt
     6721 ggcccgttac gcaggcatgg cgtcggtaaa atatatcgcg cgaatgccac agcagagaaa
     6781 actggccgta ctcacagcat tcgttaaagc gcaggagact gcggcgctgg atgaggccgt
     6841 tgatgtgctc gatatgctga tactggatat cactcgtgcg gcgaagaaaa cggggcagaa
     6901 aaaacggctc aggacgctta aagatcttga tcgtgctgcg ctaatattgg cgcgggcatg
     6961 ttcattgttg ctggatgaac aggctgacga tgctgaactg agggagacca tattcaacag
     7021 cataccgaaa agcaggctgg cagaatccgt cagcaaggta aatgagctgg cccggcctca
     7081 gaacaacaat ttccatgacg aaatggtgga gcagtacggg cgggtaaagc gctttcttcc
     7141 ggcggtattg cgcgacctgc atttccaggc agcgccagcc ggagaacata cgttgtccgc
     7201 cattcattat ctgaccgaac tgaacggctc gaaaaagcgc atcctggaca atgcgcctga
     7261 acatattatt accggcccct ggaaacgcct ggtatacgat gcggagggcc ggatacagcg
     7321 tgccggttac tcgctttgtc tgctggagcg ccttcaggat gcactacgtc gaagggacat
     7381 ctggctcgaa aacagcgatc gctggggaaa tccccgcgag aagctgttgc agggggaaga
     7441 atggcaggct cagcgggtcc ccgtttgccg ggcgctggga catcccactg atggacataa
     7501 aggcgtgcaa cagttggcgg tccagctaga tgaaacctgg aaaaccgtcg catcccgctt
     7561 tgaaggaaat gcggaggttc atatttgcca tgacggtaaa catccttccc tgactatcag
     7621 cagcctggag aaactggagg agccaacatc gttgcatcgt ctcaacagtc gggtaaggct
     7681 gctactcccg ccagtagatt tgacggaact gttgcttgaa atagatgcca gaacgggatt
     7741 tacacgtgag tttacgcatg tcagtgaatc cggggcccgg gcacaggatc tgcacatcag
     7801 cctgtgcgcg gtcctgatgg cagaggcctg caatatcggg ctggaaccgc tgataaagca
     7861 caatataccg gcgctgacgc gccaccggct tagctgggta aaacagaatt acctccgggc
     7921 agaaacgctg gtcagcgcca atgcccgcct ggttgatttt cagtccacac tggagcttgc
     7981 tggccgctgg ggtggtggcg aagtggcttc agctgatggc atgcgctttg tcacgccggt
     8041 gaaaaccgtc aattcaggac ctaacagaaa atattttggt tccggacgtg gcatcacctg
     8101 gtacaacttc gtctctgatc agtactctgg attccacggc atcgttgtcc ccggcacatt
     8161 acgagattcc atttttgtgc tggaaggcct tctggagcag cagacagggc tgaatccggt
     8221 tgagatcatg acagacacag ccggtaccag cgacattatt tttggcctct tctggctgct
     8281 gggataccag ttttcccccc ggcttgccga tgccggtgaa gcggtattct ggcgagtgga
     8341 taaatcggca aattacggtg cactggacga actggcacgt ggttgtgccg atctgtcgaa
     8401 agccgaggat cagtgggatg agatgatgcg aaccgccggt tcgctgaaac tgggcaccat
     8461 tcatgcttca gaactcattc gctctttgct gaaaagctcg cgcccatcag ggctggcaca
     8521 ggcgatcatg gaagtggggc gcgtcaacaa gacgctgtac cttcttaatt atattgatga
     8581 tgaggattat cgtcggcgga tcctgacgca gctaaaccgg ggggaaggcc gccatgctgt
     8641 ggcgagggcg atctgctacg ggcagcgcgg tgagatcaga aagcgctatc gtgaaggtca
     8701 ggaagatcag ctgggggcac tgggcctggt cactaacgca gtggtactgt ggaacacact
     8761 ttatatgcag gaagccctga gctggatacg cagtaatgga gaagaaatcg gggttgaaga
     8821 tatcgcccgg ttgtccccac tgatgcacgg gcatatcaat atgctggggc attatacgtt
     8881 tacgctaccg gaggatattt tgaaggggga actgagagaa ctaaatttca atttaaacaa
     8941 tgaattaact tcttagcgta cgttttcgtt ccattggccc tcaaacccca tcttgtaaca
     9001 atcaggcata ccacggagct tgtttgctt
//
LOCUS       DADLTI010000473         8707 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.162, whole genome shotgun sequence.
ACCESSION   DADLTI010000473 DADLTI010000000
VERSION     DADLTI010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.162"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            50..502
                     /locus_tag="JN755_004538"
     CDS             50..502
                     /locus_tag="JN755_004538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7383034.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            748..1792
                     /locus_tag="JN755_004539"
                     /pseudo
     CDS             748..1792
                     /locus_tag="JN755_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1908..2468
                     /locus_tag="JN755_004540"
                     /pseudo
     CDS             1908..2468
                     /locus_tag="JN755_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2525..3982)
                     /gene="pepD"
                     /locus_tag="JN755_004541"
     CDS             complement(2525..3982)
                     /gene="pepD"
                     /locus_tag="JN755_004541"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7383035.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4244..4702
                     /gene="gpt"
                     /locus_tag="JN755_004542"
     CDS             4244..4702
                     /gene="gpt"
                     /locus_tag="JN755_004542"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7383036.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4794..6038
                     /gene="frsA"
                     /locus_tag="JN755_004543"
     CDS             4794..6038
                     /gene="frsA"
                     /locus_tag="JN755_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7383037.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6096..6497
                     /locus_tag="JN755_004544"
     CDS             6096..6497
                     /locus_tag="JN755_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7383038.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6536..7591)
                     /gene="phoE"
                     /locus_tag="JN755_004545"
     CDS             complement(6536..7591)
                     /gene="phoE"
                     /locus_tag="JN755_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7383039.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7879..>8707
                     /gene="proB"
                     /locus_tag="JN755_004546"
     CDS             7879..>8707
                     /gene="proB"
                     /locus_tag="JN755_004546"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7383040.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     "
BASE COUNT         2120 a         2132 c         2230 g         2225 t
ORIGIN      
        1 aaaacactat cagtaagttg gagtcattac ccgtttttga atttatatta tgaataacat
       61 acaaataaga gattatcagc ctggcgattt tcagcaacta tgcgctattt tcattagagc
      121 ggttacgatg accgtcagtc agcattattc accacaacaa attgccgcct gggcgcagat
      181 tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagtcattaa
      241 tgcaaaactg gtcggcttta ttacctgcgt tgaacactat atcgatatgt tatttgttga
      301 ccctgaatac acccgccgtg gggttgccag cgctttgtta aaacctttta ttaagtctga
      361 atccgaactt acggtggacg caagcataac cgcaaaaccc ttttttgaac gttatgtttt
      421 tcagacagtt aagcagcagc gcgttgaatg ccggggagcg tggtttatta atttttatat
      481 gcgttataaa ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg cctagtatgt
      541 gcgcgttttc ttatttcaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg
      601 actctttgca gacctttcca ggattaattc tttttttctt gccctggatt cgtctgccat
      661 ttcctgattt tttaattgtt actctcaata attcaagtcg cagcacatgc aacttgaagt
      721 atgacgagtc tatttacgga atggtatatg ggcaattata ttcgtccctt atccgatgcg
      781 gtatttacca tcgcatctga tgacctgtgg atcgagagtt tagcgatcca acaattacac
      841 accacggcaa atttacccaa catgcagcgc gtagtgggga tgccagattt acaccccgga
      901 cgcggctacc cgattggcgc agcgttcttc tccgcaggcc gtttttaccc agcactggtc
      961 ggcaatgata tcggctgtgg catggcgcta tggcaaacag atattctcgc tcgcaaatac
     1021 aacgccgata agtttgaaaa gcgattatca gctttggatg acgttgctga agaaagctgg
     1081 ctggaggaaa acctgccgtc agcatttgca cagcatccgt ggcgcagctc gcttggctct
     1141 atcggtggtg gcaatcattt cgctgaactg caacaagttg atcaaatcat caacgctgaa
     1201 ctgtttgcac tggcaggtct ggatgcgcag catctgcaac tgctggttca tagcggctcg
     1261 agggagctag ggcagtctat tttacagcgg catattgcct cgttttcgca tcatggtttg
     1321 cctgaaggca gtgacgacgc gctggcgttt gcgcgtttta atcgccacct gatcgctttg
     1381 cgcataatgc aacaggttaa ggccaccgga agtccggttc tggatgtggc gcataacttt
     1441 gttagcgcgt gtcgaatcgg tgatcaacaa ggggtcttac atcgtaaagg tgccacgcct
     1501 gatgactgtg gtctggtggt tattcccggt tcacgcggtg attactccag gctggttcag
     1561 gcccgtcagg agtgaggaaa cattgcattc gctggcgcat ggggctgggc gtaaatgggg
     1621 gcgcaccgag tgtaaagggc gtctggcagc gaaatacaca gcgacgcaac tctcgcgtac
     1681 tgaacttggc agtcgggtaa tttgtcgcga taaacaactc atcttcgaag aagcgccaca
     1741 agcctataaa tcggctgaaa gtgtggtgca atgtctgggc aggccggatt aattattcct
     1801 gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct
     1861 tgctataact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc
     1921 tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ctggaaacag
     1981 aaacaggccg ctactctgac acgctgcgtt cggcgctgat ttctctggat ggcgacaacg
     2041 catgggcgtt aagcgaaagc tggtgtggca ctattcagtg gatttgcccg agcccgtatc
     2101 gcccacatca cgggcgtaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc
     2161 aggagcaatc cgatgcgatc cggtatgaga cgctgcgttc gtcggggccg ggcggtcaac
     2221 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
     2281 aggttcagtc agagcgcagc cagcatgcta acaaacggct ggcgcgattg ctgattgcct
     2341 ggaagctgga tcagcagcaa taggaaaata gcgcggtgct gaaatcacag cggcgaatgt
     2401 tccatcacca gattgaacgt ggcaacccgc gacgaacgtt tacagggatg gcttttatcg
     2461 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
     2521 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta
     2581 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
     2641 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     2701 taatctggat gttcggcgtc ttgttgaaca ggcgctgata ggtttcacgt accagatgca
     2761 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
     2821 cgccagccag tttacccagc gaatccagca tgctcaccac gtagtcttta ccgctgtcga
     2881 tcagtgaacg gatcaggcag tgaatttcta cgttattgtc agtcatggtc accacaccga
     2941 cgttcaggga ggtttcgacc acaccttttg ccacgtcgga attgcggatc acaccgttcg
     3001 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
     3061 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
     3121 tcaggatctc ctgataggta ttcaccagag atttcagtgc gtcgacttta tcagctgtga
     3181 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     3241 cgataaggcg caggtccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
     3301 tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta
     3361 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     3421 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     3481 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     3541 cggcttcttc ggttatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     3601 ccagaaccgc cagtgcagag gccataccaa tgccgttatc cgcacccagc gtggtgccgc
     3661 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     3721 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     3781 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     3841 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     3901 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     3961 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     4021 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtatt
     4081 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     4141 tgaaatactg gtttttagtg cgccagatat ctataatctc gcgcaaccta ttttcccctc
     4201 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     4261 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     4321 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gggcgttact
     4381 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     4441 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     4501 tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa tgtatccaaa
     4561 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     4621 tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt
     4681 cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg ttgttctttt
     4741 taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     4801 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     4861 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa
     4921 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc
     4981 cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg
     5041 atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat gagtgggcca
     5101 cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgcaa ctcagtggtc
     5161 gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag
     5221 atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc
     5281 gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg
     5341 gctttttgca tatgccgcaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc
     5401 tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca
     5461 ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc
     5521 aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg
     5581 atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg
     5641 cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc
     5701 tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga
     5761 gtcgtttggg gatgcatgat gcttccgacg aagcgttacg cgtggagctg aatcgctatt
     5821 cattaaaagt gcaaggattg ctggggcgtc gctgcccaac gccaatgtta tcaggctact
     5881 ggaagaacga tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg
     5941 acggtaaatt attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc
     6001 aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa ttttgccaat
     6061 ttggtaaaac agttgcatca caacaggaga tagcaatgac gttaccgagt ggacacccga
     6121 agagcagatt gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg
     6181 aagataatcg attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg
     6241 aagtgcgtga attctggggc tggtggatgg agctggaagc gcaggaatcc cgttttacct
     6301 acagctacca gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag
     6361 atactgaagt ggttgaacga ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg
     6421 aactgctgac gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gatgaaccgg
     6481 tgaagttaac ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat attcattaaa
     6541 actgatacgt catcccaacc gcgacagtat catcattatt aatattcagc ttattatcgc
     6601 tatccagttt gttgatttta taatcaacaa acgctgacat atttttgttg aaataatacg
     6661 tagcaccgac gtcgatataa ttgaccagat cttcatcacc gatgccctca atatctttcc
     6721 ctttcgataa gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcga
     6781 ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgtg
     6841 tttcagaata gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc
     6901 atgcttctgc acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg
     6961 agttggtata ggccccacta atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg
     7021 tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt
     7081 gcagggttaa gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca
     7141 gaccgctggc gcgtttggtc ataaagttgt cggtctgcgc cgaggaatcg ccaccaaatt
     7201 ccgggaacat atcggtccag gcttccacgt catacaacgc gcccaggtta cgaccataat
     7261 cgaaagaacc caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg
     7321 cagtatcact ctccgcttta tttccggcaa actccgcttc ccaccgacca taaccagtca
     7381 gttgatcgtt aatttgagtt tcacctttaa aaccaaaacg gatataactc tggtcgccat
     7441 ctttactggc gttatcactc atataatgca tggctttaac tttgccatag acatccagtt
     7501 tattaccgtc tttattatat atttctgcag cctgtacaga tgcagatgcc acaatgccca
     7561 tcaccactaa tgccagagtg ctctttttca ttttcattcc tgattttaat taacgcgcga
     7621 atattcagcg ggagagcccc gttgaaaaca ggaaagtttt taacctgaga ttgttaaaga
     7681 tatattacag attaatgata ttctaaaaat gtggtaattt attaaatctg taataaaagc
     7741 gtaaacaact gccgctacgc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca
     7801 gatggttggc aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa
     7861 attgaatggc agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt
     7921 gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata tcgttgaact tgttcgccag
     7981 tgcgcgcagt tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc
     8041 gccggacgtg agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg
     8101 ctggcggcgg tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat
     8161 ggcattcacg tcgggcaaat gctgctgacc cgtgctgata tggaagaccg tgaacgcttc
     8221 ctgaacgccc gcgacaccct gcgtgcgttg ctcgatcaca atatcgttcc ggtaatcaat
     8281 gagaacgatg ctgtcgctac ggcagagatt aaggtcggcg ataacgataa cctttctgcg
     8341 ctggcggcga ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg
     8401 tataccgctg atccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt
     8461 gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg
     8521 agtactaaat tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc
     8581 gcgggcagca agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg
     8641 ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg
     8701 gcgggtg
//
LOCUS       DADLTI010000474         5715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.217, whole genome shotgun sequence.
ACCESSION   DADLTI010000474 DADLTI010000000
VERSION     DADLTI010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.217"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            <1..75
                     /locus_tag="JN755_004547"
     CDS             <1..75
                     /locus_tag="JN755_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7383041.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            72..335
                     /locus_tag="JN755_004548"
     CDS             72..335
                     /locus_tag="JN755_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7383042.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            328..714
                     /locus_tag="JN755_004549"
     CDS             328..714
                     /locus_tag="JN755_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7383043.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            722..1408
                     /locus_tag="JN755_004550"
     CDS             722..1408
                     /locus_tag="JN755_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7383044.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1386..2009)
                     /gene="tetR(B)"
                     /locus_tag="JN755_004551"
     CDS             complement(1386..2009)
                     /gene="tetR(B)"
                     /locus_tag="JN755_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7383045.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2091..3296
                     /gene="tet(B)"
                     /locus_tag="JN755_004552"
     CDS             2091..3296
                     /gene="tet(B)"
                     /locus_tag="JN755_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7383046.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3409..4002)
                     /gene="tetC"
                     /locus_tag="JN755_004553"
     CDS             complement(3409..4002)
                     /gene="tetC"
                     /locus_tag="JN755_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7383047.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4090..4500
                     /locus_tag="JN755_004554"
                     /pseudo
     CDS             4090..4500
                     /locus_tag="JN755_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4516..>5715)
                     /locus_tag="JN755_004555"
     CDS             complement(4516..>5715)
                     /locus_tag="JN755_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY7383048.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1581 a         1056 c         1243 g         1835 t
ORIGIN      
        1 ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata
       61 aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta gctggtttta
      121 tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg ctatcttggt
      181 gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg aaagctcaac
      241 aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat attactcgcg
      301 aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt agataatcaa
      361 ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg attggaaggg
      421 ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga acaaggaaag
      481 gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg gacaccatta
      541 attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg agtattggcg
      601 gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact gatattaata
      661 acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa gtgaacttgc
      721 gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg cgataggggc
      781 ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg ggcgagcttg
      841 gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga gcagtcattt
      901 atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc atcgttattt
      961 tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga tctccttaaa
     1021 ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac gtacttgcta
     1081 tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc aacagcaatg
     1141 gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc atgaaatggg
     1201 aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag attggagtga
     1261 acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt atgaatctaa
     1321 agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa aaggttatgc
     1381 tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat gatcaattca
     1441 aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtcgt
     1501 aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc gacttgatgc
     1561 tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag tgcatataat
     1621 gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc gagagtttca
     1681 tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg atgacttagt
     1741 aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct ttccccttct
     1801 aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa
     1861 gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag cttctgggcg
     1921 agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa tgcgctgtta
     1981 atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat
     2041 cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa atgaatagtt
     2101 cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc cttatcatgc
     2161 cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct aaccactttg
     2221 gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg cttggaaaaa
     2281 tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc gcatcgctgg
     2341 attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt
     2401 cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat accacctcag
     2461 cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt ggtttaatag
     2521 cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc ttttttatcg
     2581 ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt gaaaccaaaa
     2641 atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat tcggtataca
     2701 tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg caattgatag
     2761 gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga tggaatagca
     2821 tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc caagcctttg
     2881 tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc ggatttattg
     2941 cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta gttttccctg
     3001 ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga gtgatgtcta
     3061 tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc cttaccaatg
     3121 caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca ctaccaattt
     3181 gggatggctg gatttggatt attggtttag cgttttactg tattattatc ctgctatcga
     3241 tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt gcttagttat
     3301 ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa cccaagaggg
     3361 cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt atttatcttt
     3421 caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat cggcaagctc
     3481 ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta agtttttaag
     3541 cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga caatggcgtt
     3601 taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac caatgatagg
     3661 catcacttct tggataggga taaggttttt attattatca ataatataat caagataatg
     3721 ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg ttgtgatgta
     3781 ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt ttttattttt
     3841 aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct catctattga
     3901 aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca gtgattgata
     3961 ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt taaaataaca
     4021 taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta agaacattta
     4081 ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa taacatccaa
     4141 ttaagatatg atgataagca gtttcataca acggttatca aagatgttct attatggatt
     4201 gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc gggttatacc
     4261 aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc tagctatatt
     4321 cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa aactatcctt
     4381 gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg atttaagtac
     4441 atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt ggaggcaatg
     4501 cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc
     4561 tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa
     4621 aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg
     4681 gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg
     4741 aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg
     4801 gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat
     4861 tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt
     4921 tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca
     4981 atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat
     5041 ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact
     5101 agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata
     5161 ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata
     5221 ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat
     5281 gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg
     5341 gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac
     5401 cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt
     5461 gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg
     5521 acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg
     5581 caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg cgtggcaagc
     5641 caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc aacttactga
     5701 tagtgtttta tgttc
//
LOCUS       DADLTI010000475         4539 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.240, whole genome shotgun sequence.
ACCESSION   DADLTI010000475 DADLTI010000000
VERSION     DADLTI010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.240"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN755_004556"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN755_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7383049.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN755_004557"
     CDS             1227..1760
                     /locus_tag="JN755_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7383050.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..1986)
                     /locus_tag="JN755_004558"
                     /pseudo
     CDS             complement(1757..1986)
                     /locus_tag="JN755_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN755_004559"
     CDS             complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN755_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7383051.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN755_004560"
     CDS             complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN755_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7383052.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN755_004561"
     CDS             complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN755_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7383053.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3204..4232
                     /gene="epmB"
                     /locus_tag="JN755_004562"
     CDS             3204..4232
                     /gene="epmB"
                     /locus_tag="JN755_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7383054.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1166 a         1142 c         1126 g         1105 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacttg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagagagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcatc aaacggctaa agccgtgggt
     1921 atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca
     1981 ggacatatca ggctccggaa atttggggcc gtccccggtg aaaagaggcg tttgcaggtc
     2041 gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat
     2101 tacctttatg acgatcgcag aaaaaacaaa cggcacgaca ctgctgtcgt accgttattg
     2161 cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt
     2221 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
     2281 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
     2341 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat ctgatgattt
     2401 ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga gatgtttgat
     2461 gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
     2521 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
     2581 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
     2641 gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag tagacagggt
     2701 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
     2761 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
     2821 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcatcagc
     2881 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
     2941 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
     3001 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
     3061 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
     3121 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
     3181 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
     3241 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
     3301 tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc
     3361 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatcctcttt
     3421 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc tccaccgacc
     3481 cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
     3541 ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc
     3601 cctatgccga aaatcagggc aacaagcgta actggcagac tgcacttgag tatgttgctg
     3661 cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
     3721 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
     3781 ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct
     3841 ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc aatgaggtag
     3901 atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc
     3961 agagcgttct gttacgtggt gtgaacgata acgcacaaac gttggcaaac ctgagtaatg
     4021 cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctagataaa gtacagggcg
     4081 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacat
     4141 tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa
     4201 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt taaacattaa
     4261 aattgcctga tgcgctacgc ttatcaggcc tacatcgtgc attgaaattg aatggatttg
     4321 caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa
     4381 gtacagcaac ctgtcattaa actgagaaat ttaatctttg cacgttgcat aacctttaat
     4441 tattcatatg cattgctgtt gttacttttt atatagctaa caccaataat taacctttct
     4501 tttcggtaat gactccaact tactgatagt gttttatgt
//
LOCUS       DADLTI010000476         1962 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.322, whole genome shotgun sequence.
ACCESSION   DADLTI010000476 DADLTI010000000
VERSION     DADLTI010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.322"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            109..939
                     /locus_tag="JN755_004563"
     CDS             109..939
                     /locus_tag="JN755_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY7383055.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1052..1843
                     /gene="aadA1"
                     /locus_tag="JN755_004564"
     CDS             1052..1843
                     /gene="aadA1"
                     /locus_tag="JN755_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7383056.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          614 a          433 c          435 g          480 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1141 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1201 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1261 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1321 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1381 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1441 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1501 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1561 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1621 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1681 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1741 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1801 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     1861 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     1921 tgcggtgatg ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DADLTI010000477         1822 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.336, whole genome shotgun sequence.
ACCESSION   DADLTI010000477 DADLTI010000000
VERSION     DADLTI010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.336"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            264..923
                     /locus_tag="JN755_004565"
     CDS             264..923
                     /locus_tag="JN755_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7383057.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JN755_004566"
     CDS             complement(1124..1501)
                     /locus_tag="JN755_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7383058.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>1822)
                     /locus_tag="JN755_004567"
     CDS             complement(1568..>1822)
                     /locus_tag="JN755_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7383059.1"
                     /translation="FEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSL
                     LQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          470 a          434 c          448 g          470 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aa
//
LOCUS       DADLTI010000478          753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS031795
            SAMEA1030104-rid11416003.guided.405, whole genome shotgun sequence.
ACCESSION   DADLTI010000478 DADLTI010000000
VERSION     DADLTI010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030104
            Sequence Read Archive: ERR047385
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942075.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,568
            CDSs (total)                      :: 4,510
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 380
            CDSs (without protein)            :: 380
            Pseudo Genes (ambiguous residues) :: 0 of 380
            Pseudo Genes (frameshifted)       :: 196 of 380
            Pseudo Genes (incomplete)         :: 87 of 380
            Pseudo Genes (internal stop)      :: 129 of 380
            Pseudo Genes (multiple problems)  :: 30 of 380
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030104-rid11416003.guided.405"
                     /strain="ERS031795"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Viet Nam"
                     /collection_date="2002"
     gene            58..531
                     /locus_tag="JN755_004568"
     CDS             58..531
                     /locus_tag="JN755_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7383060.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          220 a          159 c          182 g          192 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccaggat gagaaccttg
       61 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
      121 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
      181 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
      241 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
      301 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
      361 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
      421 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
      481 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
      541 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
      601 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
      661 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggacggcact
      721 gttgcaaata gtcggtggtg ataaacttat cat
//
